LOCUS       DAMZPP010000001        64043 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMZPP010000001 DAMZPP010000000
VERSION     DAMZPP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 64043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64043
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.001"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            16..513
                     /locus_tag="POH73_000001"
     CDS             16..513
                     /locus_tag="POH73_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDI6885995.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISANIEILL
                     QESSS"
     gene            complement(621..713)
                     /locus_tag="POH73_000002"
     CDS             complement(621..713)
                     /locus_tag="POH73_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6885996.1"
                     /translation="MSVINAYSHASLLFFIYLLFIMYQISLSVK"
     gene            904..2784
                     /locus_tag="POH73_000003"
     CDS             904..2784
                     /locus_tag="POH73_000003"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HDI6885997.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQETSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEED
                     AEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGWDPQSPVPINDHGDT
                     VPIAEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAHEYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESERF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAETILRQMKQRQRYQRD
                     VY"
     gene            2850..3458
                     /locus_tag="POH73_000004"
     CDS             2850..3458
                     /locus_tag="POH73_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HDI6885998.1"
                     /translation="MSMPLMIDLTNKNVVIVGGGTVASRRAQTLSQYVEHMTVISPTI
                     TEKLQNMVDKGVVIWKGKEFEPSDIVDAYLVIAATNEPRVNEAVKQALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKLLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVRQ"
     gene            3531..3644
                     /locus_tag="POH73_000005"
     CDS             3531..3644
                     /locus_tag="POH73_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6885999.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKIRRTNLIK"
     gene            3947..5317
                     /locus_tag="POH73_000006"
     CDS             3947..5317
                     /locus_tag="POH73_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HDI6886000.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVI
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWFGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKPWIKWVNTALTLAVILAMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            5564..7414
                     /gene="nrdD"
                     /locus_tag="POH73_000007"
     CDS             5564..7414
                     /gene="nrdD"
                     /locus_tag="POH73_000007"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDI6886001.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPNWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYTSG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            7411..7947
                     /gene="nrdG"
                     /locus_tag="POH73_000008"
     CDS             7411..7947
                     /gene="nrdG"
                     /locus_tag="POH73_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDI6886002.1"
                     /translation="MTLLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDKILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            8047..8156
                     /locus_tag="POH73_000009"
                     /pseudo
     CDS             8047..8156
                     /locus_tag="POH73_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8172..8333)
                     /locus_tag="POH73_000010"
     CDS             complement(8172..8333)
                     /locus_tag="POH73_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886003.1"
                     /translation="MAIIEYLYNFSRNKQNFNSAKTTVLSYDLTNLTNFTRMAFKNIY
                     TLLTNIYLL"
     gene            8465..10087
                     /locus_tag="POH73_000011"
     CDS             8465..10087
                     /locus_tag="POH73_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDI6886004.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            complement(10257..10820)
                     /locus_tag="POH73_000012"
     CDS             complement(10257..10820)
                     /locus_tag="POH73_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HDI6886005.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINPLLESSDLTEAETQEAHKIKAQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQSSDSKN"
     gene            complement(10908..11003)
                     /locus_tag="POH73_000013"
     CDS             complement(10908..11003)
                     /locus_tag="POH73_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886006.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            11072..12562
                     /gene="betB"
                     /locus_tag="POH73_000014"
     CDS             11072..12562
                     /gene="betB"
                     /locus_tag="POH73_000014"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDI6886007.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEIDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            12823..14532
                     /gene="betA"
                     /locus_tag="POH73_000015"
     CDS             12823..14532
                     /gene="betA"
                     /locus_tag="POH73_000015"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDI6886008.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            14810..15022
                     /locus_tag="POH73_000016"
     CDS             14810..15022
                     /locus_tag="POH73_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDI6886009.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            15302..15745
                     /locus_tag="POH73_000017"
     CDS             15302..15745
                     /locus_tag="POH73_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDI6886010.1"
                     /translation="MNIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDTL
                     CVLNHLYINEGQEETFEQKFLQRNKHLQNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADML"
     gene            15939..17537
                     /locus_tag="POH73_000018"
     CDS             15939..17537
                     /locus_tag="POH73_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDI6886011.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPDSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(17597..17928)
                     /locus_tag="POH73_000019"
                     /pseudo
     CDS             complement(17597..17928)
                     /locus_tag="POH73_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18224..19720
                     /gene="mqo"
                     /locus_tag="POH73_000020"
     CDS             18224..19720
                     /gene="mqo"
                     /locus_tag="POH73_000020"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDI6886012.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            complement(19913..20803)
                     /locus_tag="POH73_000021"
     CDS             complement(19913..20803)
                     /locus_tag="POH73_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HDI6886013.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVEGKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDSLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     ANLYKDLAEHPNVVRIVVLSGGYSREKANELLKDNAELIASFSRALASDLRAGQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(20926..21342)
                     /locus_tag="POH73_000022"
     CDS             complement(20926..21342)
                     /locus_tag="POH73_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886014.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDIISNQSHLKALLLNLDF
                     LIPSDNTPYTLEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLVFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            21596..22933
                     /locus_tag="POH73_000023"
     CDS             21596..22933
                     /locus_tag="POH73_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDI6886015.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALENICRENGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKNLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            complement(23013..24461)
                     /locus_tag="POH73_000024"
     CDS             complement(23013..24461)
                     /locus_tag="POH73_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6886016.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSIAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAVTFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            25147..26106
                     /locus_tag="POH73_000025"
     CDS             25147..26106
                     /locus_tag="POH73_000025"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDI6886017.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            26384..27088
                     /gene="budA"
                     /locus_tag="POH73_000026"
     CDS             26384..27088
                     /gene="budA"
                     /locus_tag="POH73_000026"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDI6886018.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(27174..28034)
                     /locus_tag="POH73_000027"
     CDS             complement(27174..28034)
                     /locus_tag="POH73_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI6886019.1"
                     /translation="MLSVAIIGPGAVGTTIVYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HIPFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIVHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            28107..28925
                     /gene="panB"
                     /locus_tag="POH73_000028"
     CDS             28107..28925
                     /gene="panB"
                     /locus_tag="POH73_000028"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDI6886020.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEKAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGIEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            28918..29769
                     /locus_tag="POH73_000029"
     CDS             28918..29769
                     /locus_tag="POH73_000029"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDI6886021.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITVVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDKVTQYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            29771..30154
                     /locus_tag="POH73_000030"
     CDS             29771..30154
                     /locus_tag="POH73_000030"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDI6886022.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(30283..31965)
                     /locus_tag="POH73_000031"
     CDS             complement(30283..31965)
                     /locus_tag="POH73_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDI6886023.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGAIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGITDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNTEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKRQD
                     VSFKYTFDKDTELVGNMNLKLWVSTTDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSTIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGSKYDSQLIIPIVN"
     gene            32022..32130
                     /locus_tag="POH73_000032"
                     /pseudo
     CDS             32022..32130
                     /locus_tag="POH73_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32108..32695
                     /locus_tag="POH73_000033"
     CDS             32108..32695
                     /locus_tag="POH73_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6886024.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSLI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(32776..33225)
                     /locus_tag="POH73_000034"
     CDS             complement(32776..33225)
                     /locus_tag="POH73_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HDI6886025.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEDAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGIFTDKYGVRWMLHGQDYTAIQQ"
     gene            33581..34303
                     /locus_tag="POH73_000035"
     CDS             33581..34303
                     /locus_tag="POH73_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDI6886026.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            complement(34489..34764)
                     /locus_tag="POH73_000036"
     CDS             complement(34489..34764)
                     /locus_tag="POH73_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886027.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLIIVILAIIRFVHKMM"
     gene            complement(34919..35983)
                     /locus_tag="POH73_000037"
     CDS             complement(34919..35983)
                     /locus_tag="POH73_000037"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDI6886028.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTTFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     TRYLKDHHFDNVSDIIGLDA"
     gene            36201..37067
                     /locus_tag="POH73_000038"
     CDS             36201..37067
                     /locus_tag="POH73_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDI6886029.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRMDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVT
                     HSMDKILQDTTD"
     gene            37404..37682
                     /locus_tag="POH73_000039"
     CDS             37404..37682
                     /locus_tag="POH73_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HDI6886030.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLISKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            37697..38065
                     /locus_tag="POH73_000040"
     CDS             37697..38065
                     /locus_tag="POH73_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886031.1"
                     /translation="MSNKLDGINKMITAKHKQMDDLYDEKQEVKALIDESDALNHSIE
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHDIRKAEEEMIDL
                     EMRRNIEIEMVTKEENKWKQ"
     gene            38053..38349
                     /locus_tag="POH73_000041"
     CDS             38053..38349
                     /locus_tag="POH73_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HDI6886032.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFDNMKQNLDSEIQRGEVTPQ"
     gene            38682..39788
                     /locus_tag="POH73_000042"
     CDS             38682..39788
                     /locus_tag="POH73_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HDI6886033.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTETYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNDGQYHISFDSGQSVLTPHEPIVATGFDATKNPI
                     VQQLFATTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQEVIENYQKNQMYLDDYSCCEVSCTC"
     gene            39782..41122
                     /locus_tag="POH73_000043"
     CDS             39782..41122
                     /locus_tag="POH73_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HDI6886034.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAVIDQTGFKSWMIWAIEPSMLRIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAEKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            41222..42112
                     /locus_tag="POH73_000044"
     CDS             41222..42112
                     /locus_tag="POH73_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDI6886035.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVVDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVSQMTATSDNKHESLHKTINHQFVQ
                     SPAKCTKAQFIKRLACLPTHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(42259..43089)
                     /locus_tag="POH73_000045"
     CDS             complement(42259..43089)
                     /locus_tag="POH73_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6886036.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELAEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWSKFEIRQYTHSDITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(43109..44119)
                     /locus_tag="POH73_000046"
     CDS             complement(43109..44119)
                     /locus_tag="POH73_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDI6886037.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLGVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEAGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSS
                     NN"
     gene            complement(44217..44921)
                     /locus_tag="POH73_000047"
     CDS             complement(44217..44921)
                     /locus_tag="POH73_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI6886038.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSTPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIDA
                     DGPTGTFINGNGEMPW"
     gene            complement(44914..45471)
                     /locus_tag="POH73_000048"
     CDS             complement(44914..45471)
                     /locus_tag="POH73_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6886039.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDHKALL
                     QCIEAGNNKLRFYQSAFYQELFYFYYDLFKSDDDIYTKFKTDMLLQALSTRVFDFQIE
                     HRNISIEAYRNYLLNIYLDEVERND"
     gene            45602..45901
                     /locus_tag="POH73_000049"
     CDS             45602..45901
                     /locus_tag="POH73_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HDI6886040.1"
                     /translation="MSLNKEQRRITAEELQAHFEESTLSVQMIAGKLNVTTEDVEKAL
                     AMKAPLGIFSHQLQRFIHLVWDVRDVINDNIKENGQTPEPYTYLKGEKEDYWFLR"
     gene            45920..46741
                     /locus_tag="POH73_000050"
     CDS             45920..46741
                     /locus_tag="POH73_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HDI6886041.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDSVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYVFEHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQDYNK"
     gene            46758..47135
                     /locus_tag="POH73_000051"
     CDS             46758..47135
                     /locus_tag="POH73_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6886042.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(47298..47528)
                     /locus_tag="POH73_000052"
     CDS             complement(47298..47528)
                     /locus_tag="POH73_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDI6886043.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(47748..48248)
                     /locus_tag="POH73_000053"
     CDS             complement(47748..48248)
                     /locus_tag="POH73_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886044.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICPCLLANVRHSSIVCLNNTC
                     RIVDTRCLNTKIGGIGVSETDEPQGFEHTHNRLNINQNSQSLMTFITSKLKSKLKQHI
                     IIARGNKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMAIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            complement(48446..49015)
                     /locus_tag="POH73_000054"
     CDS             complement(48446..49015)
                     /locus_tag="POH73_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6886045.1"
                     /translation="MTESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSGSFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            49170..49299
                     /locus_tag="POH73_000055"
                     /pseudo
     CDS             49170..49299
                     /locus_tag="POH73_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            49426..50481
                     /locus_tag="POH73_000056"
     CDS             49426..50481
                     /locus_tag="POH73_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDI6886046.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGILILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            51089..51790
                     /gene="isaA"
                     /locus_tag="POH73_000057"
     CDS             51089..51790
                     /gene="isaA"
                     /locus_tag="POH73_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HDI6886047.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVPASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            52476..54287
                     /locus_tag="POH73_000058"
     CDS             52476..54287
                     /locus_tag="POH73_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI6886048.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKSQFARTVLVILLLIPSI
                     IVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            54648..55415
                     /locus_tag="POH73_000059"
     CDS             54648..55415
                     /locus_tag="POH73_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6886049.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWASAAASSGYTVNNTPKAGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            55596..56084
                     /gene="crtO"
                     /locus_tag="POH73_000060"
     CDS             55596..56084
                     /gene="crtO"
                     /locus_tag="POH73_000060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HDI6886050.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKHIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHKLNRNIYDQRHLMTINIDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRVVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            56077..57570
                     /locus_tag="POH73_000061"
     CDS             56077..57570
                     /locus_tag="POH73_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6886051.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDDFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGTRVDNIKTYERCVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLLHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            57576..58703
                     /gene="crtQ"
                     /locus_tag="POH73_000062"
     CDS             57576..58703
                     /gene="crtQ"
                     /locus_tag="POH73_000062"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HDI6886052.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETACVARSYGATVVDVVDDADGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVEALINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMAAIVLWLFGSIASILGLCLSLKYRQMSVGKMLTVYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            58740..59603
                     /locus_tag="POH73_000063"
     CDS             58740..59603
                     /locus_tag="POH73_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HDI6886053.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEYHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFENERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFRDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVEKRKKAKL
                     FHEINSKYHRI"
     gene            59615..61123
                     /gene="crtI"
                     /locus_tag="POH73_000064"
     CDS             59615..61123
                     /gene="crtI"
                     /locus_tag="POH73_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HDI6886054.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTMCGKNYEDYIELRQLRYIYDVYFDRDDCITVPTDL
                     AELQHMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFADD
                     FRGNIEEIFEGRLSHDPSIYVYVPAVADKSLAPQGQTGIYVLMPTPELKTGSGIDWSD
                     EALTDQIKDVIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIEQGV"
     gene            61550..62704
                     /locus_tag="POH73_000065"
     CDS             61550..62704
                     /locus_tag="POH73_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6886055.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSKIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKDTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKARRDRFEAMLTKADLPFFHAKGGIYVWLETPPGYDSEQFEQ
                     FLVKEKSILVAPGKPFGENGNRYVRISLALDDQQLDEAAIRLTELAYLYE"
     gene            62727..63725
                     /locus_tag="POH73_000066"
     CDS             62727..63725
                     /locus_tag="POH73_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDI6886056.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNLIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKDAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVENLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(63815..64021)
                     /gene="copZ"
                     /locus_tag="POH73_000067"
     CDS             complement(63815..64021)
                     /gene="copZ"
                     /locus_tag="POH73_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDI6886057.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSAEVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
BASE COUNT        21895 a         9746 c        12080 g        20322 t
ORIGIN      
        1 gtgtaggggt gtcttatgaa taatagaaat gtgtatgata tcgaagtaag tgattataaa
       61 ggcttaactt ataaattaga agcatttaga ggtaaagtga ttttagttgt taatactgca
      121 acagaatgta tatatagcga acaattgaaa aaactagaga ctttgtttca aaaatataag
      181 gatcgtgggt ttgtagtgtt gagttttccg aataataatt ttgacaatcg acaaccaggt
      241 tctaatgaag aaatcttgaa aattagtcgt gagaaatttg gtgttacatt tccagtgcta
      301 gctaaaatat ctgtgaacgg gaacgatgaa catccgttat ttacgcattt aaagaatgaa
      361 caaccaggaa tctttgggtc cccaataaaa tggaatttca caaaatttat aatcgatcga
      421 caaggcaatg ttgtaaatcg attcttgcca atggaagatc cattggatat atcggcaaat
      481 atagaaatat tattgcaaga atcttcatct taaatttaaa attgagcgct tagtatgcaa
      541 ataacacaag agcctcttaa tgatgtacta atagtatgtc attaggaggc attttatatt
      601 tagaaaatca gtaatttgac ctattttact gaaaggctga tttggtacat aataaatagt
      661 aaataaataa aaaataacaa cgatgcatga ctgtatgcat taataactga cacgacataa
      721 tcaaagtttt gtctgaatta ctgtgtcatt caaaccacat gaaggttgta aatatttgta
      781 agtataacta tcagacacaa ctcaagtttg ttttaattta taccatacat attaaaaaat
      841 tgactgaact atcgatgaaa aatgagtcaa tcatctattt caagtttatt gaggtgaaaa
      901 tatttgaaat taaatacatc taatagtcca tttacagaaa aacaagtcac agagattaac
      961 aatctgctgc aaacattaac agagagccaa caacagtggc ttagtggttt tttactagct
     1021 aatagtaacg atacgacaag tgatagtaat caacaacaat tagagacaga agtgtggcaa
     1081 caatcacaaa tatcagaaga acaagaaact tcaacaacgt atatgttaca aaataaagag
     1141 ccacatatcg aagctaatca gcggcatgtc acagtgctat atggttctga atcaggtaat
     1201 gccatgcgtt tagctgaaat tttttcagaa cgattaagtg atattggaca tcaagttgtt
     1261 ttgatgtcaa tggatgaata tgatacgaca aacatcgcac agttagaaga tttatttatt
     1321 attacgtcta ctcatggtga aggagaaccg cctgataatg catgggattt ctttgaattt
     1381 ttagaagacg ataacgcacc taatttaaat catgtgagat attcagtact agctttaggt
     1441 gatcaaacat atgaattttt ctgtcaagcc ggtaaagatg tagatgcttt actagaaaat
     1501 ctaggcgctg agcgtatatg taagcgtgta gattgtgata ttgattatga agaagacgca
     1561 gaaaagtgga tggcagacat cattaatatt attgatacca catcagaagg tattcaaagt
     1621 gaatcggtga taagtgaatc aattaagtct gccaaagaaa agaaatattc taaatcaaat
     1681 ccataccaag cagaagtatt agcgaatatc aatttaaatg gtaccgattc aaataaagaa
     1741 acacgacata tagaattttt acttgatgat tttagtgaat catatgaacc aggagattgt
     1801 atagtagcat taccgcaaaa cgaccctgaa ttggttgaaa aactaatatc catgttaggt
     1861 tgggatccgc aatctccggt gccaattaat gatcatggtg atacagttcc tattgctgaa
     1921 gcactaacat cacattttga atttactaaa ttaacattgc cattattgaa aaatgcagat
     1981 atctattttg acaatgaaga attatctgaa cgtattcaag atgagtcatg ggcgcatgaa
     2041 tatgttataa atcgggactt tatagattta ataacagatt ttccaactat agaattacaa
     2101 cctgagaata tgtatcaaat ccttagaaaa ttaccaccaa gagagtattc gatttctagt
     2161 agttttatgg caacgccaga tgaagtgcat attaccgttg gtacggttcg ttatcaagca
     2221 catggacgtg agagaaaagg tgtatgctcg gttcattttg ctgagcgtat taaaccaggc
     2281 gatatagtac caatttattt gaagaaaaat ccgaacttca aatttccgat gaagcaagat
     2341 ataccggtaa ttatgattgg tccaggtact ggtattgctc cttttagagc atatttacaa
     2401 gaacgtgaag aacttggtat gactggaaaa acatggttgt tctttggtga tcaacaccgt
     2461 agttctgact ttttatatga agaagaaata gaagaatggc ttgaaaatgg aaacttaaca
     2521 cgcgtagatt tagcattttc aagagaccaa gaacacaaag aatatgtaca gcatcgtata
     2581 atggaagaaa gtgaacgttt caatgaatgg attgagcagg gcgcagcaat ctatatttgt
     2641 ggcgatgaaa aatgtatggc gaaagatgtc catcaagcca ttaaagatgt attggtaaaa
     2701 gaacgtcata tttctcaaga agaagctgag acgatattgc gtcaaatgaa acagcgacaa
     2761 cgttatcaac gcgatgttta ttaatgcttc aatgtgttaa atgttttaag gtgaaatgac
     2821 gtaaaaagat ataaaggatg tagcgcaata tgagtatgcc attgatgata gatttaacaa
     2881 ataagaacgt ggttatagta ggtgggggta cagttgcaag tcgtcgggca caaacattaa
     2941 gtcaatacgt tgaacatatg acggtcatca gtccgacaat cactgaaaaa cttcaaaata
     3001 tggtagataa aggtgtcgtc atatggaagg gaaaagagtt tgaaccaagc gatattgtag
     3061 acgcgtatct agttattgca gcaaccaatg agccacgtgt caatgaagcg gtaaaacaag
     3121 ccttacctga gcatgccctt tttaataatg ttggagatgc atcaaatggc aatgttgtat
     3181 ttccaagtgc actacaccga gacaagctaa ctattagtgt atctacagat ggcgcaagtc
     3241 ctaagttgac aaaatcgatt atggcagagc ttgaggcgtt atatccacca tcatatagtt
     3301 cgtatatcga ctttttatat acttgccgac agaaaataaa gttactagat ataacatata
     3361 acgaaaagca acagttactg tcacaaattg tgtcacaaga atatttaaat catgacaaac
     3421 aagctcaatt tttagcgtgg ttggatgtaa gacaataata gcgggccgtc taaccgtcta
     3481 aggtaagtct tcttatttta actttaacgc ttaatcattg aaattaagac atgggtggct
     3541 ttgtgaatag tctaataatg aaggatttaa gcgataatga tatgcgtttt aaatatgaat
     3601 attacaatag agaaaaaata cgtagaacaa acttaataaa atagatggat aaattgaaat
     3661 ctggttgaag tcgtgactat catagcgacc tttagccaga ttttttgtgc aatagaaagc
     3721 aataataaaa atgataaatc aaaatgaaat acaggacagg atatacaagg attagtcatg
     3781 ccatgttatc aagtaggaaa atcaaacttc actattgata gttacacaaa aaagaaattt
     3841 ttgataaaat gagttaactt aaatataaaa aattatatta attattatat ttaagttaaa
     3901 gagggggatt atgtaaattg tattaaaagt ggagggagaa aataatatga atagtgataa
     3961 tatgtggtta acagtaatgg ggctcattat tattatttca attgtaggtt tactcattgc
     4021 caaaaagata aatccagttg taggtatgac aatcatacct tgcttagggg caatgatttt
     4081 aggatatagt gtgacagatt tggttggatt ttttgctaaa gggttagatc aagtcatcaa
     4141 cgttgttatt atgtttatct ttgccattat tttctttggc atcatgaacg atagtggttt
     4201 attcaagccg cttgtcaaac gcttaatatt aatgacacga ggcaatgtca ttattgtctg
     4261 tgcaatgaca gctttaattg gcacaatagc ccaattagat ggtgccggtg cggtaacatt
     4321 tttgctttct attcctgcct tattaccttt atataaagcg ttaaatatga ataaatattt
     4381 attgattcta ctattagcat taagcgcggc gattatgaac atggtacctt ggggaggtcc
     4441 gatggcgcgg gtagctgctg ttttaaaagc taaaagtgtc aatgaattat ggtttggatt
     4501 aatacctatt caaataatag gtttcattct tgttatgtta tttgcggttt atcttggatt
     4561 taaagaacaa aagcgtatta aaaaagcaat agagagaaat gaattaccgc aaacacaaga
     4621 tatagatgta cataaattag ttgaagtata tgaacgagat caagatgtaa ggtttcctgt
     4681 aaaaggacgt gcacgaacaa aaccatggat aaaatgggtg aatacagctt taactttagc
     4741 tgttattcta gcgatgttaa taaatattgc gccacctgaa tttgcattca tgataggtgt
     4801 ctcattggcg cttgttatta attttaaatc agtggatgaa caaatggagc gattaagagc
     4861 gcatgcgccg aatgcattaa tgatggctgc agtgattatt gcagcaggta tgtttttagg
     4921 tgtactaaat gaaaccggta tgcttaaagc gattgcgacg aatttaatca aagtgattcc
     4981 tgcagaagta ggaccatact tgcatattat tgtaggttta cttggcgtac cattagattt
     5041 attaactagc acagacgctt attattttgc tgtgttaccg atcgtcgaac aaacagcagg
     5101 gcaatttggt gtaccatctg tatcaactgc ttattcaatg gtcataggga atattatagg
     5161 tacatttgtc agtccgtttt caccagcctt atggttggca attggtttag cagaggcaaa
     5221 catgggcacg tatattaagt atgcattctt ttggatttgg ggattcgcca tcgttatgtt
     5281 agtaattgca atgttgatgg gaattgtgac gatttaagta tgaaaaaata gaaactatgc
     5341 tcccgttaca aaatgaaata atagttgcat aaagatgtcg aaatgacgga acgattcgag
     5401 aacaatttta aaaattaatg aaataattgt gtagaaatat gaatttcact aaatgttaat
     5461 aactttgtga cgttttagtt aacagactaa taaaaatttg aaaatactat atatagtggt
     5521 ataacgtaat gagtagacac aatatatagg aagaaggggt aaaatgaatc aaatcgaaga
     5581 agcattaacg ggtttgattt ctaaagatcc tgctattgtt aacgaaaatg ccaacaaaga
     5641 tagtgataca ttttcaacaa tgagagattt aacagcaggt atcgtttcta aatcttacgc
     5701 attaaatcat ttattaccaa agcatgttgc agatgcacat caaagagggg acatacattt
     5761 tcacgactta gattatcatc cattccaacc attaacaaac tgttgtttaa tagatgctaa
     5821 aaatatgcta cataatggat ttgaaatagg caacgcgaat gtaacttcac caaaatcaat
     5881 acaaactgca tcagcgcagc ttgtacaaat tatagccaat gtttctagca gtcaatatgg
     5941 tggctgtacg gttgaccgcg ttgacgaatt acttagtaca tatgcacgac ataatgaaga
     6001 acaacatagg aatatcgcaa agcaatttgt caaagaatct gaaattgatc gttatgttga
     6061 tcaacaagtc actaaagaca tcaatgatgc gattgaaagt ttagaatatg aaattaatac
     6121 cttatataca tctaatggac agacaccttt tgtaacatta ggattcggct taggtacaga
     6181 tcatttaagt cgcaaaattc aacaagctat cttaaatact cgtatcaaag gcttaggaaa
     6241 agaccgcacg acagcgattt tcccgaaact tgtattttca attaaaaaag gaaccaactt
     6301 tagtccgcaa gatccgaact atgacattaa acaattagca ttaaagtgtt caacgaaacg
     6361 tatgtatcca gatattttaa attatgacaa actcgtagaa atattaggtg atttcaaagc
     6421 gccaatgggt tgtcgttcat ttttaccaag ttggaaagat gcggaaggtc attttgaaaa
     6481 taatggtcgt tgtaatcttg gtgttgttac acttaattta cctagaatgg cattagaatc
     6541 tgccggtaat atgacgaaat tctgggaaat cttttatgaa cgtatcgatg tgttacatga
     6601 tgcattactt tatcgtataa atcgtttgaa agatgctgta ccgaataacg caccaatttt
     6661 atataaaagt ggcgctttta actataaatt aaaagaaaca gatgatgttg ctgagttatt
     6721 taaaaataaa cgtgcaacga tttcaatggg ctatataggg ttgtatgaaa cagctactgt
     6781 tttctatggt ccaaactggg aaacatctca agaagcaaaa gcatttacgc ttgaaattct
     6841 taaagaaatg aaacgttatc aaacgaaatg gacagaatta tatgacattt ggttcagtat
     6901 ttacagtacg ccgagtgaat cgctaacgga tcgtttttgt cgtttagacc aagagagatt
     6961 tggagatatt aaagacatta cagataaagg atattaccaa aactctttcc attatgatgt
     7021 acgtaaagat gttacacctt ttgaaaagtt agattttgaa aaagattatc cttattatac
     7081 gagtggtggt ttcattcact attgtgagta tccgaaactg caacacaatt tgaaagcact
     7141 agaagcagta tgggactact cttatgacaa agttggttac ttaggtacaa atattccgat
     7201 tgatcattgt tatgaatgtg attacgatgg agattttgaa gctactgaaa aaggatttaa
     7261 atgcccgaac tgtggcaatg ataatcctaa aacagttgat gtcgttaaac gaacatgtgg
     7321 ttacttaggc aatccagttc aacgtccagt aattaaaggc cgtcataaag aaatttgcgc
     7381 acgagtaaaa catatgaaag cgcctaaaga atgacacttt tagacattaa acaagggcaa
     7441 ggttatattg ctaaaataga atcaaatagc tttgttgacg gtgaaggagt aagatgcagt
     7501 gtttatgtat caggatgtcc atttaattgt gttggatgtt acaacaaagc ctcacaaaag
     7561 ttcagatatg gcgagaaata cactgataaa atattagcag aaatattaga tgattgcgat
     7621 catgattata tatctgggct aagtctatta ggtggcgaac cattttgtaa tttggatatt
     7681 acattaaatc ttgtcaaagc atttcgagca cgttttggaa atacaaagac aatttgggta
     7741 tggactggat ttttatatga atatttagca aatgattgta cagaacgtcg agagttatta
     7801 tcatacattg acgttttagt agatgggcta tttatacaac acttattcaa acctgattta
     7861 ccatataaag gttctttaaa tcaacgcatt atagatgtac aacaatcact ctcgcatgcg
     7921 cgtatgattg aatatattgt tagttgaata tgtattagaa gtcaaggtaa catccgttgc
     7981 cttggcttct ttttaggtta ggcacataat tgaaagttga taaaagcaat tctttagaaa
     8041 aacatattga tagaatatga cctaacaatc attttgatac taatactaaa agttgcatat
     8101 acattttaaa aaaagttgaa agagaaaagt ggtactttag tgggaaggaa gtctaacttt
     8161 ttggtagcgt tttacaataa ataaatattc gttaataacg tataaatatt cttaaatgcc
     8221 atcctagtaa aatttgttaa attcgttaaa tcataactta acacagttgt tttagcgcta
     8281 ttaaagtttt gtttattacg agaaaaatta tataaatatt caataattgc caactttcaa
     8341 attgtatgaa atttgcatta ttattaaata ttagttattg tcaattttgt gaatcaatat
     8401 aattattaca ttttgagata aatcgaaaca ggattcataa aattaataat tagggggagc
     8461 acaattgaaa aaagagaaag ttatggactg gacgaccttt atagggacag tagctgtact
     8521 tctttttgca gttataccta tgatggcttt tccaaaagca agtgaagata tcatcactgg
     8581 tattaatagt gccatttctg attcaattgg ttcgatatat ttatttatgg ggctggcgat
     8641 tttttgcttt gtaatgtata tcgcatttgg taaatatggt aatgtcacac ttggtaaagc
     8701 aagtgataaa ccagaattta atacatttac atgggcggca atgctgtttt gtgcaggcat
     8761 aggctctgat attttatact ggggcgttat tgaatgggct ttttactatc aagttccacc
     8821 aaatggcgcg aaaagtatga gtgatgaagc actccaatat gcgacgcaat atggtatgtt
     8881 ccactgggga ccaattgctt gggctattta cgttttacca gcattaccaa ttggttattt
     8941 agtgtttgtt aaaaaacaac cggtgtataa aattagtcaa gcttgtcgcc cgattttaaa
     9001 aggtcaaaca gataaatttg taggtaaagt tgtagatatc ttatttatct ttggattgct
     9061 aggtggtgcg gcaacatcac tagcgttagg tgtgccatta atttctgcag gcatagaaag
     9121 attaactggt ttagatggta aaaatatgat tttacgttcg gccattttat taacaatcac
     9181 ggttatattt gccattagtt catatacagg attgaaaaaa ggtattcaaa agttaagtga
     9241 tatcaacgtt tggctatcat ttgtactttt agcctttata tttattattg gaccgactgt
     9301 ttttattatg gaaacgacag tgacagggtt cggaaatatg ttgagagatt tcttccatat
     9361 ggcaacatgg ttagaaccat tcggtggtat taaaggtcga aaagaaacga atttcccaca
     9421 agactggaca atattctact ggtcatggtg gttagtatat gcgccattta tcggtttatt
     9481 tatcgctaga atttcaaaag gtcgacgcct taaagaagtc gtgctaggaa caattattta
     9541 tggaacgctt ggatgcgtat tattctttgg tatttttggt aactatgctg tgtatttaca
     9601 aatttctgga cagtttaatg taacacaata tttaaataca catggtacag aggcaaccat
     9661 tattgaagtg gtgcatcatt taccattccc atcattgatg attgtactat tcttagtatc
     9721 tgctttctta ttcttagcaa caacatttga ttcgggttca tatattttag cggcagcatc
     9781 tcagaaaaaa gtggtaggcg aaccattacg tgccaatcgt ttattctggg cgtttgcatt
     9841 gtgcttattg ccattttcat tgatgctagt tggtggtgaa cgtgcattag aagtattgaa
     9901 aactgcatca atactggcaa gtgtgccatt aattgttatt tttattttca tgatgatatc
     9961 atttttaatc attttaggac gcgatagaat taaacttgaa acgcgggctg aaaaattaaa
    10021 agaagttgaa cgtcgctcat tgcgaatcgt tcaagtatca gaagaagaac aagacgataa
    10081 tttataattc aattaatgat gcataagatt ttgaatttta taagtatatg attaatcgtg
    10141 atattaagca aaaacggtaa tttctattta ttgagataga aattaccgtt tatttcgtgg
    10201 acctattgca ttgttttcat catgcataat catcattgtc gttgtttgag tcaattttaa
    10261 tttttagaat cagaagactg ttctggaatt gggaaatatt taaaaatttc accgctttca
    10321 atcgcttcgg ttaactgttc taaccattcg taataaacat gtgtatgatc aagctgagct
    10381 ttaattttgt gtgcctcttg tgtttcagct tcagttaaat cactgctttc aagtaatgga
    10441 ttgataatag cttgagcatc ttttactgct tcgacattga tatcaatttc acgctgaaat
    10501 tttttagtga aaaagtttcg gaaaaagatg aaaaagtctt tctcggcgat aaaatgttgt
    10561 ttgcggcttc ctctcgtaaa ttgttgttta acaatatcaa attcctgcaa tttcttaacg
    10621 ccagcactca tacttggttt gctcatttgc aattgatgac gcatttcatc aagcgtcata
    10681 ctgccttcaa acaccatagt gccatataag tttcctacac ttctattagt gccatacaaa
    10741 tccattgtct gtccaattga attaattaca atatcttttg cttgttctaa ttgttgctgt
    10801 ttgttctgag aacgagtcat cattgcacct ccgtacatca ttttggtcac gttaaaataa
    10861 atactaatac attataaaac cttttctaaa aaagacatta aaaatattta aagcattaaa
    10921 gttaaatgtt tcgttaaata aaaatctaac gaacttacaa aacttaattc ttgagttgtt
    10981 ttgtaaattg acacattttt catttctatg ctaacataag tttgtaaaat tcgttaaata
    11041 aaaatttaac aaacttaacg gaggttgttg aatggaactt ttaaaacatt tatctcagcg
    11101 tcaatatatt gatggtgagt gggttgaaag cgcgaataaa aatacaagag atattatcaa
    11161 tccttacaat caagaagtga tatttacggt ttctgaaggg acaaaagagg atgcagaacg
    11221 tgcaatctta gctgcaagac gtgcgtttga gtctggtgaa tggtcgcaag aaactgctga
    11281 aacaagaggt aaaaaagtac gtgccatcgc ggacaagatc aaagaacatc gcgaagcgtt
    11341 agcacgatta gaaacattag atactggaaa aacgttagaa gaatcatatg cagatatgga
    11401 tgatattcat aatgtgttta tgtattttgc tggattagca gataaagatg gtggcgaaat
    11461 gattgattca ccaataccag atacagaaag caaaattgtt aaagaaccag taggtgtagt
    11521 tacacaaatt acaccttgga attatccgtt attacaagca tcatggaaaa ttgcgccagc
    11581 gcttgctacg ggttgttcac tagttatgaa accaagtgaa attacaccat taacaacaat
    11641 acgtgttttt gaattaatgg aagaagttgg tttccctaaa ggaacaatta atcttattct
    11701 tggtgcaggt tctgaagttg gtgacgtaat gtcaggtcat aaagagattg acctagtatc
    11761 atttacaggt ggcattgaga ctggtaagca tattatgaaa aatgctgcta ataatgttac
    11821 gaatattgcc ttggaacttg gcggtaaaaa tccaaacatt atctttgatg atgctgattt
    11881 tgaattggcg gtagaccaag cgttaaatgg tggatatttc catgcaggtc aagtttgttc
    11941 agcaggatca agaatattag tacaaaacag tattaaagac aaatttgagc aagcacttat
    12001 tgatcgcgtg aaaaaaatca aattaggtaa tggttttgat gctgatactg aaatgggacc
    12061 agtgatttca acagaacatc gtaataaaat cgaatcttat atggatgtag ctaaagcaga
    12121 aggcgcaaca attgctgttg gtggtaaacg cccagataga gacgatttaa aagatggtct
    12181 attcttcgag ccaacagtca ttacaaattg tgatacgtca atgcgtattg tacaagaaga
    12241 ggttttcgga cctgtcgtta ctgtagaagg ctttgaaact gaacaagaag cgattcaatt
    12301 agcgaatgat tctatatatg gtttagcagg tgctgtattt tctaaagata ttggaaaagc
    12361 acaacgcgtt gctaacaagt tgaaacttgg aacggtgtgg attaatgatt tccacccata
    12421 ttttgcacaa gcgccatggg gtggatacaa acaatcaggt atcggtagag aattaggcaa
    12481 agaaggctta gaagagtacc ttgtttcaaa acacatttta acaaatacaa atccacaatt
    12541 agtgaattgg tttagcaaat aaaaattaga taaggtgagt gccattgtaa gaacacaaga
    12601 cactcacttt gttttgtata agtggcgaaa tgttgattga taatttggac taaacgcaaa
    12661 atgaatcata gattatttca tgactgttag taacaatcat aaaaggaaaa tcgagtgttt
    12721 tggttagcta agtttagcaa ttcaacgata accaatcagc cactaacaaa tatttcatgc
    12781 aatactcact ttgaaataca acaaactttg gaggtcataa cgatgagtaa caaaaataaa
    12841 tcatatgatt atgtcatcat tggaggaggc agtgcaggtt ctgtactagg taatcgtctg
    12901 agtgaagata aagataaaga agtcttagta ttagaagcgg gtcgcagtga ttatttttgg
    12961 gatttattta tccaaatgcc tgctgcgtta atgttccctt caggcaataa attttacgat
    13021 tggatttatt caacagatga agaaccacat atgggcggtc gtaaagtagc acatgcccga
    13081 ggtaaagttt taggtggatc aagttcgatt aatggcatga tttatcaacg cggtaatcca
    13141 atggactatg aaggctgggc agaaccagaa ggtatggaaa cttgggattt tgcgcactgt
    13201 ttaccgtatt ttaaaaaatt agaaaaaaca tacggtgcag cgccttatga taaatttaga
    13261 ggccatgatg gaccaattaa gttaaaacga gggccagcaa cgaatccttt attccagtca
    13321 ttctttgatg caggtgttga agcaggctat cataaaacac ctgatgtgaa tggatttaga
    13381 caagaaggtt ttggaccgtt cgatagtcaa gtacatcgtg gtcgccgaat gtcagcttca
    13441 agagcatatt tacatccagc tatgaagcgt aaaaacttaa ccgttgaaac acgtgccttt
    13501 gttactgaaa ttcattatga aggtagaaga gcaactggtg ttacgtataa gaaaaatggc
    13561 aaactacata ccatcgatgc taatgaagtc atcttgtcag gtggggcatt caatacgcca
    13621 caattactac aactgtctgg tatcggcgat tcagaattcc taaaatcaaa aggcattgag
    13681 ccacgtgttc atttgcctgg tgtgggtgaa aactttgaag atcacttaga ggtatacatt
    13741 caacataagt gtaaggaacc tgtatcatta cagccaagct tagatatcaa gcgcatgcca
    13801 tttattggtt tacaatggat tttcacacgt actggtgcag cagcatctaa ccattttgaa
    13861 ggtggcggat ttgtacgttc aaataacgaa gttgattatc ctaacttaat gttccatttc
    13921 ttaccaatag ctgtaagata tgatggtcaa aaggcagcgg tcgcacatgg ctatcaagtt
    13981 cacgtaggac caatgtattc aaactctcgt ggcagcttga aaatcaaatc taaagatcca
    14041 ttcgaaaaac caagtatccg ctttaattat ttatcaacag aagaagataa aaaagaatgg
    14101 gtagaagcaa ttcgtgtagc aagaaatatc ttatctcaaa aagcaatgga tccattcaat
    14161 ggtggagaaa tttcaccagg accagaagtg caaacagacg aagaaattct tgattgggtt
    14221 cgtagagatg gtgaaacagc attacaccca tcttgtagtg ctaaaatggg tcctgcttca
    14281 gatccaatgg cagtagttga cccgttaaca atgaaagttc atggtatgga aaatttacgt
    14341 gtagttgatg catcagctat gccacgtacg acaaatggca atatacacgc acctgtttta
    14401 atgttagctg aaaaagcagc agacattata cgtggcagaa agcctttaga gccacaatat
    14461 attgattatt ataagcatgg tgttcatgat gaaaatgaag gtgcaatcga agtaaaacca
    14521 tacgctaaat aaattgatat agtagacttt tagtctggca tctattgtat tggtgccagg
    14581 cttttttgcg ttttatctaa atgagaagtt aatgtgttgt tcaaaaacgg tgtgtgagaa
    14641 tatgaactga aaatagagtt aattgtacat tgtgatgatt aattgaccta attgacagac
    14701 attaaaagag tgtacaacct taaaaagttc actaaataga catttaattg tgttttcttg
    14761 aaagctttcg tggtaggatt tatataagta aattgagaga aggaatacta tgagctttta
    14821 cgaatatata caaacattta aagatgataa aacaccatta ggcgaattag cgatttggat
    14881 taaagaagat gattcattcc caaaacaaga gaagctaaca gaaaacattt tgtcttattt
    14941 tcatcaaatg tccaatatag atcatgagtt tttagaaatt gtaaaaagat cactttctct
    15001 gtatgatcaa ttaaaatcgt aaaattagag ataaagttag ctaaaataca aggccggtaa
    15061 tttcatagat gaagttgtcg gctattttca tgactaatgt taaataatga tcatggaatt
    15121 gaagacacaa aaataatggg cgacgcctgt ccccatttgt atggaatatt aatacttttt
    15181 acaaagcctt atcttttaaa aagaagtcaa actatgattg taattaataa atacgggaat
    15241 gaattagtaa tagatataaa gtaatatgac caaaaatata aatgaaatga aggcatttat
    15301 tatgaacatt tatgatgaat atagaagtta tgtaatagaa gaactggatg attgtctcac
    15361 aatacaaaaa aataatgaca ctgcatatta cgatgtgcta gaagcgataa atgatttatc
    15421 aaatgataca ttatgtgtgt taaatcattt atatattaac gaaggacaag aagaaacatt
    15481 cgagcaaaaa tttttgcaac gtaataagca tttgcaaaac gttgatgggt ttaaagcatt
    15541 aaggttttta agacctagaa cagctggtag acactatatc attattacgc tgtgggaaaa
    15601 taggcaagca ttttatcatt ggcaaaattc agctgagtac aagcatacac acaagcatcg
    15661 tggcacttcg aaaggtgcag atgtaaaaat aattaatcgt gaattatcat acaatattcg
    15721 tattgaatta gctgatatgc tctagcccga gatatcaacc ctcaatcatt tgaaagtcct
    15781 tgaatgctat gtcatgtcaa agcgtagtgt cgaggacttt ggtatttaac cctatttaat
    15841 tgaaaattct gtaaagatag gcgtatgata tatataattc atttataaaa ttacaatgtt
    15901 catcacagga aaagtggatg tatgaaagga agttagttat gcaaaagtca gctctattcg
    15961 cgcctgaaaa atataacatt atttcggaaa tcgaaaaata tagccacata ccagataaaa
    16021 aggcaatttt atatcataat actgaaggcg aagatatatc agtcacttac caacagctca
    16081 ttgaacagtc taataaagta ggtaatgttt tagcatcgca cggtttatct aaaggcgata
    16141 aagtactcat tatgatgcca cgatctattg cgacatatga attatatatt gctgctttaa
    16201 aattaggtgt tgcaatcatt ccatgttctg aaatgctaag aactaaagat ttacaatacc
    16261 gtatcacaca cggtgaaata aatgctgtta ttgcactaga gccattcaca gttgaatttg
    16321 aaaaaattaa agaatatgat gcgttaacta aatttgttat tgccggtcaa aaagatggat
    16381 ggatttcact tgaagctgaa aaagaaaacg caagtaacaa actagaaatg gcagatacga
    16441 cgcgtgatga tatggcgatt ctgtcatata catctggtac aactggcaat cctaaagccg
    16501 ttacacattg tcatggatgg ggatttgcac atttacaaat ggcaccaaaa cattggttat
    16561 gtattaatga agatgactta gtttgggcaa cagcagcacc aggttggcaa aaatgggtat
    16621 ggagtccttt cttatctgta ttaggttcag gtgcaactgc ttttgtattt aatggaagat
    16681 tcagccctga gacatactta gaattgcttc aaaaatatca aattaatgtt ttatgttgta
    16741 caccgacaga atatcgtatg atggctaaat tacagaactt aaatgattat gacttaacgc
    16801 atttacatag tgccgtttct gcaggtgagc cactaaatcg agaagtagtt gaacaattca
    16861 agaaatactt taatttaact gtaagagatg gatatggcca gactgaaagt accttgctta
    16921 taggcttttt aaaagataca ccacaaagga ttggatcaat gggtaaaggc attccggata
    16981 gttcggtcac tgtagtagat gatgagggga atagcgtacc tgctaatacg aaagggaata
    17041 ttgcagtacc acttgatttg ccagcacttt tcaaaggata ttataaagag ccagaacgta
    17101 cggcgaaagc gcaaacaggt aaatactaca tcactggtga cttagctcat gtagatgatg
    17161 atggttattt ctggtttgaa ggacgtcgag atgacattat tattagttca ggctatacga
    17221 ttgggccttt tgaagttgaa gatgcgttaa cgaatcatcc agccgttaaa gaatgtgctg
    17281 tagtagcaaa acctcatgat atacgaggaa atattgttaa agcattcgtt attttacagg
    17341 accatactgc gggtgatgat acgctagtta aagaactaca acaatttgtt aaaaatgaag
    17401 ttgcaccata taaatatcca agagagattg aattcgttga cgacttacca aaaacaaact
    17461 caggtaaaat tcgtcgtgtt gaactacgag atgctgaaat agaaaaatgg caacaacaaa
    17521 aagatagcaa tcaataaaca tagcaaagca gtccaatgcg taaggtatga tgatacgcac
    17581 tggactgctt gtttatttat gatgttcttt gtcgtctagt tataaatatt aaaattagtt
    17641 tagaaacaag cgcaaccagt attaagaatg agaaaatata agatgttaaa cctcgaatat
    17701 cccagtttaa taaaaaaaca ttactatcac cggcattaag ttgaaactca gtatgtgtta
    17761 aaacgataat cacaatgcct ataagtgcaa gattcaaaat aatttacaat attaaaggta
    17821 attgaagtga ttttcttgga tgcataggca ttgaatctcc tttttatgat tgattttaca
    17881 cttatattgt atcaaaatag agtataggtt taaaatgcta taagccaaat gtagattaaa
    17941 acaatcgtta gctatttgtt gtcaaataat tatgattgat ttgatattta tcttctatga
    18001 ataaaagatt taatatattg caaagttaat gaataattat aatttacatt taccactgct
    18061 tgctttaaca tatacatgta agcactaact atagtatttt gttacagcgt atcaattaaa
    18121 acaggaagtt atctatggcg ttgatatttt aaagaaatat gtcagtttta tgtaactttc
    18181 atattgatga tacacattta caatttttgg agaggtgaaa ccaatggcta agtctaatag
    18241 taaagacatc gttttaattg gagccggtgt acttagcaca acatttggtt caatgttaaa
    18301 agaaattgag ccagactgga atatccacgt ttacgaacgc ttggatcgtc ctgcaatcga
    18361 aagttcaaac gaaagaaata atgctggtac gggtcatgca gcattatgtg agttgaacta
    18421 cacagtttta caacctgatg gttctatcga catcgaaaaa gcgaaagtga ttaacgaaga
    18481 gtttgagatt tcaaaacaat tctggggtca cttagtgaaa agcggtagca tcgagaaccc
    18541 aagagaattt atcaatccat taccacacat cagttatgtt agaggtaaaa acaatgttaa
    18601 attcttaaaa gatcgttacg aagcgatgaa agctttccct atgttcgata atatcgaata
    18661 tactgaagac atcgaagtaa tgaaaaaatg gattccattg atgatgaaag gccgtgaaga
    18721 taaccctggt atcatggcgg caagtaaaat tgacgaaggt acagatgtaa acttcggtga
    18781 attaacacgt aaaatggcta aaagcattga agcacatcca aatgctactg tgcaatttaa
    18841 ccatgaggtt gttgattttg aacaattatc aaatggtcaa tgggaagtta ctgttaaaaa
    18901 tcgcctaact ggtgagaaat tcaaacaagt aactgactat gtattcatcg gtgctggcgg
    18961 tggagcaatt ccattattac aaaaaacagg tatccctgaa agtaaacatt taggtggatt
    19021 ccctatcagt ggtcaattct tagcttgtac aaacccacaa gttattgaac aacacgatgc
    19081 caaagtttat ggtaaagagc cacctggtac accaccaatg actgtacctc atttagatac
    19141 gcgttacatt gatggtcaaa gaacattatt atttggacca tttgctaatg ttggacctaa
    19201 attcttgaaa aatggttcta acttagattt attcaaatca gttaaaacat acaacattac
    19261 aactttatta gcagcagctg ttaaaaactt acctttaatt aaatactcat ttgatcaagt
    19321 gattatgaca aaagaaggtt gtatgaacca cttacgtact ttctatccag aagcacgtaa
    19381 tgaagattgg caattataca ctgctggtaa acgtgtacaa gttatcaaag atacacctga
    19441 acacggtaaa ggattcatcc aattcggtac agaagtggtt aactcacaag accacactgt
    19501 aatcgcatta ttaggtgaat caccaggggc ttcaacttca gtttcagttg cgttagaagt
    19561 attagaacgt aacttccctg aatacaaaac tgaatgggca cctaaaatca agaaaatgat
    19621 tccatcatac ggtgaatcat taattgaaga cgaaaaatta atgagaaaaa tccgtaaaca
    19681 aacttcaaaa gacttagaat taggttacta cgaaaactaa attctaatag ttatatttaa
    19741 aatcggacaa ctgagaaatc ttaaagattt ttcagctgtc cgattttttt acgtaatcta
    19801 atacatacgg caaaatcaac tttaatcaaa aagactcata cacaatgcct ttaaagcaca
    19861 tgtatgagtc ctttttagta gtttatatca aaaaatacgt ttaatgtata aattagtttt
    19921 tgtttacaga tgcgtcgtag attgattcta cagcgtcacc taatgcttta tcgaattctt
    19981 ctttagattg tccagctctt aaatcactag ctaatgcgcg tgagaagctt gcgataagtt
    20041 cagcgttatc tttaagtaat tcattagctt tttctctgct gtaaccacct gataatacaa
    20101 cgatacgaac aacgtttgga tgctcagcta agtctttgta taagttagct tcagtaggaa
    20161 ttgttaattt taacataact aattgatcag catttaagct gtctaaacct tttttaagct
    20221 cagcttttaa tactttttca atttcagctt tatcttttgc gttaatatta acttctggtt
    20281 cgataattgg cactaaccct ttagcaataa tttgtttagc gacttcaaat tgttgttcaa
    20341 caacgtcttt gataccttgt tcatttaatt ctaaaatatt agaacgcatt tttgtaccaa
    20401 aaatgtgacg ctcgtttgca cgatctaata agctatctaa gttgtcgatt ggtttcatta
    20461 attgaacacc attttgctct tcagcaagac ctttgtctac ttttaagaac ggaacaacac
    20521 ctttgtcagc taagtaatct gcagtgtatt tgccttctac ttcgcgatcc attgtttgtt
    20581 cgaaaagaat agcacctaaa attttatctg gtgagaatga aggtgaagtt accacacgtg
    20641 tacgcatatc gtgaacaagt tggaacattt cgtcttcatt gctatattga tcttcgttta
    20701 caccatattc tttaagtgct tttggtgtac taccaccact ttggtctaat gcggcaataa
    20761 agccttttcc atttttcatt ttttctaatt gctctttatt catactttcc actccttaac
    20821 ttttcaatac acctccagta tgataaaaat gagaacattt ctcaagtcat aaaccttgaa
    20881 aagtgtataa aatgtgaaaa ataattgtca gtttaattaa aaatattatt ttattctagg
    20941 tatgactaac gccattaatg acatgaagaa aatatgtgta ataatccaac cgattaattc
    21001 tgtcacacta aattgaaaaa ttggacgttg cgcaataaat actaaaaagg gatacaatgc
    21061 tataaataag aaaactaaag ggatataaca tagatagtaa agccttttag aagtatgaaa
    21121 cagcaacaca aatacaaaat aaatgacgct accaattagt aagtgacata ttatttctag
    21181 agtatatggt gtattgtcag aaggaattag aaaatctaaa ttcaataaca aagcttttaa
    21241 atgtgattga ttgcttatta tgtcaaacaa catggtcatc catagcaaca tgaatgatat
    21301 gaaaatagca gcaatgaaat ggaacaatac ctttaaacgc attcatatca cctcagagtc
    21361 aaatggaata tagtaaacaa ttccctaatc gaacaaaatt atgcgcataa ataaagtaga
    21421 ttgatataaa attcttaatt atcagaatat atttacaaat ctgaatttta ttagtatatt
    21481 ggatagtatt cataatgaca tgacagtatt ttcacgtgat atcaaatcga atgtggttat
    21541 cgatgtaggg aaggtaatta tgtgaataag gtctgtcaat gggaggggaa tgtatatgag
    21601 taaagcacat caattaattc aagaggatga acattatttt gcgaaatcag gacgtattaa
    21661 atattatccg ttagttattg atcatggata tggagcaaca ttggttgata ttgagggaaa
    21721 gacatatatc gatttgttat cgagtgcgag ttctcaaaac gtaggtcatg cacctagaga
    21781 agtaacagaa gcgataaagg cacaagttga taaatttatt cattatacac cagcatatat
    21841 gtatcatgaa ccgttggtac gtttagctaa gaagctttgt gagattgcac ctggagattt
    21901 tgaaaaaaga gtgaccttcg gattaactgg atcagacgca aatgatggca tcattaaatt
    21961 tgccagagca tatacagggc gtccttatat cattagtttc actaatgcat atcatggttc
    22021 aacttttggc tcgttgtcta tgtcagctat tagtttaaat atgcgcaaac attatggtcc
    22081 gttattgaat ggcttttatc atattccgtt tccagataaa tatcgtggta tgtacgagca
    22141 gccacaagct aattcagtag aagaatattt agcaccctta aaagaaatgt ttgcgaaata
    22201 tgtacctgct gacgaagtag catgtattgt tattgaaacg atacaaggcg atggtggact
    22261 tttagaacca gttccagggt attttgaagc gttagaaaat atttgtcgtg aaaatggtat
    22321 tttaatcgct gtcgatgata ttcaacaagg ttttgggaga acaggtacat ggagttcagt
    22381 ctcgcatttt aattttacgc ctgatttaat cactttcgga aaatctttag caggtggtat
    22441 gcctatgtca gcaattgttg gacgcaaaga gattatgaat tgtttagaag caccagcaca
    22501 tttatttaca acaggtgcta atccagttag ttgtgaagct gcattagcca caattcaaat
    22561 gattgaagat cagtcgcttc ttcaggctag tgcggaaaaa ggggaatatg ttaggaaacg
    22621 aatggatcaa tgggtatcta aatacaatag tgtaggcgat gttagaggta aaggcctgag
    22681 cattggtatt gatattgttt ccgacaaaaa cctcaaaaca cgtgatgcca gtgcggcact
    22741 taaaatttgt aattactgct ttgagcatgg cgtagttatt atagctgtag caggaaatgt
    22801 gttgcgattc caaccgccat tggtaataac atatgagcaa ttagacacgg cgttaaacac
    22861 tatagaagat gcactgactg ctttggaagc aggaaactta gatcaatatg acatatctgg
    22921 acaaggttgg taatagcgat tatcttaata taaaataaaa aatcatttcc acatctggat
    22981 gttaatcaga tgggaaatga tttttttaat ttttattttg gtgggtggta ttcagctacg
    23041 tcatttttct tagaatgtct aagtccataa cttaaatata gaatgatacc aacaataaac
    23101 caaattaaag tgtataattt cgcttcgaat cctaatcccc agaatactag caatactaaa
    23161 acaaatgtaa ctgctggtaa cacaggatat aaaggtaatt taaacgcagg aattggtaaa
    23221 tctttacctt cacgctttct caaacgatac attgctaatg aaacgaacat aaatgcaaca
    23281 agtgtaccgg ctgaaattaa ttgtgctaaa aatgcgaatg ggaacataga accaattaaa
    23341 acaccaataa tagtaagtat aactagtgca cgattaggta aatgtttgtc gtttaagtgg
    23401 cttaaccatg aaggtaataa gccgtcacgt ccaaatgaat aaagtaaacg tgagcctgct
    23461 aacatcatac caattaatgc tgtaaacata ccgataacag agatagcttg aacaatagct
    23521 gctacaacac catgaccact ttgacgtaaa gcccaaccaa caggttcagc attgtttgcg
    23581 tattgtgagt aatggaacat accaactaac acaagtgcta cagcaataaa taatacgata
    23641 gcaatactta aagaaccaag aatacctcta ggcattgtct tttgagggtc aagtgcttct
    23701 gctgagtttg ctgcgataga atcgaaaccg atatacgcta agaaaatcat tgaaacacca
    23761 gcatatatgc cttgccatcc accaaagtca cctgtagcag ttactttgtg ttctggaata
    23821 aatggcacat agttactaac atttattgct gttaaaccta cgattacaaa taaaataata
    23881 gctaatactt ttaaaataac taaaatgttt tccatacgtg ctgcttccga cataccacgt
    23941 gatagtaata atgcagttaa taaaataacg atagcagcaa taatatcgat aaaaccgcca
    24001 tttgtaccaa atggatttga taatgctgca ggtaattcga tgccaattgg tttcacaagt
    24061 ccgcgtaaat tcgctgagaa tcctgatgca acaaaggcta cggcgataaa atattcagct
    24121 aataaagccc aaccggcaac ccatccaaag aattcaccaa ataatacatt gacccaagaa
    24181 taggctgaac ctgcaaatgg catagcggca gccatttctg cataagtaaa tgcaactaaa
    24241 ccagcaacaa tagcagcgag taagaatgat aacgcaacgg ccggtcctgc atgttctgca
    24301 gcaacaatgc caggtagcgt aaagatagat gtcgatacaa ttgttcctac acctaaagct
    24361 aagaaatcac gcacccgaag tgtacgcttt aaatgaccat ctttattttg atagatagcc
    24421 ggatcctctt ttcgtgctat tttattgaaa aaacttccca taaactttcc tcccaaacct
    24481 tcataaacaa ttctatacgg tgttttttaa tatgttatat catagcacaa ataatcaata
    24541 ttttgtctaa aaattctgaa aaatcacaac tttatgttac ttattaatga cttgtcttga
    24601 taacatccat agatttttta aatgataaaa ctgattataa cagatattaa atgaataagt
    24661 actatttttg ctaattttct aacaattttg cgcattatat ttttaaaatc aatttcatgt
    24721 ttatgtctga ttgcctagtt tgtatgaaat gtaattcttt gactataaaa aggtgcatca
    24781 aatatgatga gagtaaggcg aaaaatagga ggttatatag taagtcattt aagtcgaata
    24841 gccatccttt ttaaaatgaa aaaaatagaa agctcaatag tttgttaaag cctttaaata
    24901 gcgtcattac gcaattttag aatgctaaaa attgtcacaa atttcaaatt acgtgctaaa
    24961 gcttgagata tcaatattta ttggcgttag agtgtaattt gactccgctt acatctcagt
    25021 tttatgtttg taatttggta gcataatatt ataataaaat aaaattgtta atctttaatt
    25081 tcagtataga tatttttacg tgtagtcacg tgtaaaataa attcaattag gttaggagac
    25141 ataattatga aaacatttgg taaaaaggtt gtattaatcg gagatggatc tgtaggatca
    25201 agctatgcct ttgcaatggt tacgcaaggt gttgctgatg aatttgtaat tattgacatt
    25261 gcaaaagaca aagtaaaagc agatgttcaa gatttaaacc atggtacagt ccacagtcct
    25321 tcgccagttg atgtgaaagc aggtgaatac gaagactgta aagatgcaga tttagttgtt
    25381 attacagctg gtgcacctca aaagccaggt gaaactcgtt tacaattagt tgaaaaaaat
    25441 actaagatta tgaagagcat tgttaagagt gtaatggata gcggctttga tggatatttc
    25501 ttaatcgcgg caaaccctgt agacatttta acaagatttg taaaagaata tactggattg
    25561 ccagcagagc gtgttatcgg ttcaggtact gtattggaca gtgcacgttt acaatattta
    25621 attagccaag aacttggtgt tgcaccttca agtgttgacg ctagtattat tggtgagcat
    25681 ggtgatactg aacttgcagt ttggtcacaa gcaaatgtag caggtatttc agtatatgac
    25741 acattaaaag aacaaactgg tagcgaagct aaagcggaag aaatttatgt aaatacacgt
    25801 gacgctgctt atgaaattat ccaagctaaa gggtcaacat actatggtat tgcattagca
    25861 ttgatgcgca tttcaaaagc cattttaaat aatgaaaata atgtcttaaa tgtttctata
    25921 caattagatg gtcaatatgg cggtcacaaa ggcgtttact taggtgtacc gacattggtt
    25981 aaccaacatg gcgcagttaa aatttatgaa atgccattaa gtgcagaaga acaagcatta
    26041 ttcgacaaat cagttaaaac attagaagat actttcgatt caattaaata tttattagaa
    26101 gactaagcct attttaagta ttaattagaa atatattaat ggtaagagga tctattagtg
    26161 ttgcggtaac atgtggcact gatagatcta tttttttagc aagcattgat agagggaata
    26221 cttttgcgta aaataataat tgaataaagt attatgactc gtgaaaattt agtgacatga
    26281 atagaaatgt cgaggcgtca ttgagtataa aggaaagtag ttctgcatta atcacgaagt
    26341 agagcatgac aacgaggaat aactataggg agatggtttt ggaatgacga atgtcttgta
    26401 tcaacatggt acattaggta cgttaatggc tggcttacta gaaggaacag ctacaattaa
    26461 tgaattgtta gaacatggaa atttagggat tgcaacgtta acagggtctg atggcgaagt
    26521 aatattttta gatgggaagg catatcatgc taacgagcat aaagaattta tagaattaaa
    26581 aggcgatgag aaagtaccgt atgcatcgat tactaatttt aaagcgagta agacatttcc
    26641 attgcaacaa ttatcacaag atgatgtatt cgcacaaatt aaaaatgaaa tgttaagtga
    26701 gaatttattt tcggctgtta aaatttatgg cacatttaaa catatgcatg tacgaatgat
    26761 gcctgctcag caaccgccat atacacgttt gattgattca gcacgcagac aacctgagga
    26821 aaaaagacaa gatattcgtg gtgccattgt tggatttttt acaccagaat tatttcatgg
    26881 cgtagggtct gctggttttc atatacattt tgcggatgat gaaagagctt atggtggaca
    26941 tgttcttgac tttgaagtgg atgacgttgt cgttgagata caaaactttg aaacattcca
    27001 gcaacatttc ccggtaaata acgagacgtt tgttaaagcg aaaatagact ataaagatgt
    27061 ggcagaagaa attagagaag ctgaatagtc taatataatt aaaagacctt agcgatatta
    27121 ggaacaggtg gttctaaatg catcgttaag gtctttttat attatatatg tgcttacata
    27181 ttttgttgat acgcgcgtaa aaagctataa atagtatcaa gatatggggt gtcaagattg
    27241 tgttcacgtg cacgtcgata aataaagcct tgaatcgctt cgacttcaag aggttgctga
    27301 tgcacaatgt cgtaatacat gctcgttccc atttcatcgg gataaccttg atatatagtc
    27361 ataatagtat cgacagtttg ttctgaaaag tttaacccct ctgcctgtgc aacacgacaa
    27421 ccatctagta atagctgtct acacagtgta cgtatttcag gattatgcat gattgcaact
    27481 gtctgtctac cgagtgctgt gatagaatta atgcccaagt taactaacaa tttataccaa
    27541 atagcttgtt gaatatttgc ttctagtaca atgtcgattt gactgtcttg gaccaaatct
    27601 ctaaattgtc gagttaatgc attatcttgt atacgtaatt gataatctct gaagtgcgta
    27661 acaacatcgc ctttcttttg accacttata tagacaactg cttggcatac gtttttaaat
    27721 ggaatatgtt cgagttgacc ataaccattt tgggctaaaa tgatgagcgt gtcctcatgt
    27781 gcaagatgag ttaaatgagg aataactgca tcaagttgat gtgttttgac tgcaataata
    27841 ataacatcaa atgtgtttgt gacatcttca taacctttta caacaatatc ttgtgcaggt
    27901 gcatgtggta cagtataata tgttattgtt ttggcgtgtc tcccgataag cgttgtatga
    27961 ggcaatgatt gttgtaattc atagacaata gttgtaccga cagcgcctgg gccgataatc
    28021 gcaactgata acatcttctt ctttcctttc tataaaagat tcattatact atttctaata
    28081 atactgcaaa aagaggttga ataacattga aaacagtaag tcaattaata gatatgaaac
    28141 aaaagcaaac taaaatttct atggtaacag cttatgattt tccaagtgct aaacaagtgg
    28201 aagcagcggg tattgatatg attcttgttg gggattcact tggtatgact gtattaggtt
    28261 atgaaagtac cgtacaagtg acgttagcag atatgattca tcacggtcgt gcagtaagaa
    28321 gaggcgcacc aaatacattt gtagttgttg atatgccgat tggtgcagta ggcatctcta
    28381 tgacacaaga cttaaatcat gctctaaagc tttatcagga gacgaatgca aatgcaatta
    28441 aagcagaagg agcgcatatt acaccattta ttgaaaaagc aacggcaatt ggtattcctg
    28501 ttgttgcaca tttaggatta acacctcaaa gtgttggcgt tatggggtat aaattacaag
    28561 gtgcaacgaa agaagcagcg gagcaactta ttttagatgc gaaaaatgta gaaaaagctg
    28621 gtgcagttgc gttagtactt gaagcaattc caaatgattt agcagaggaa attagcaagc
    28681 acttaacaat tccagtcatt ggtattggtg caggaaaagg tacagatggt caagtattgg
    28741 tttatcacga tatgttaaat tatggtattg aacataaagc gaaatttgtg aagcaatttg
    28801 ctgattttag tgttggtgtg gatggtttaa agcaatacga tcaagaagtt aaatctggtg
    28861 catttccatc agaagaatat acgtataaaa agaaaattat gaatgaggtt aataacaatg
    28921 actaagctga ttactacggt aaaagagatg caacacattg ttaaagcagc caaacgctca
    28981 ggcacaacga ttggttttat cccaacaatg ggtgccttac atgatggaca tttaacgatg
    29041 gtacgcgaat cagttagtac aaatgatatt acagttgtga gtgtatttgt taatccatta
    29101 caatttggtc caaacgaaga tttcgatgct tatccgagac aaattgataa agatttagaa
    29161 ttagtatcag aagtaggggc ggacattgtt tttcatcctg cagtagaaga tatgtatcca
    29221 ggtgaattag gtattgatgt caaagtaggt ccattagctg atgtgttaga aggagcgaag
    29281 cgtccagggc attttgatgg ggtggtaaca gtagttaata agctatttaa tattgttatg
    29341 ccggattatg cttattttgg taaaaaagat gcccagcaat tggctattgt cgagcaaatg
    29401 gtaaaagact tcaatcatgc cgttgaaatt attggtattg atatcgttcg agaagcagat
    29461 ggtttggcga aaagttcaag aaatgtttat ttaacggagc aagaacgaca agaagcggta
    29521 catttaagta aaagtttgct attagcacaa gcgttgtatc aagacggtga acgtcaaagt
    29581 aaagtaatta tagacaaggt tactcaatat cttgaatcac atataagtgg acgcattgaa
    29641 gaagttgctg tttatagtta tcctcaatta gtagaacaac acgaaataac tggacgcata
    29701 tttatctcgt tagctgtcaa attttcaaaa gcgcgtttaa tagataatat aataattgga
    29761 gctgaataaa gtgataagaa caatgatgaa tgctaaaatt catagagcaa gagtgactga
    29821 gtcaaattta aattatgtag gtagcattac gattgattca gatatattgg aagcggtaga
    29881 catcttgcca aatgaaaaag tagccatcgt gaataataat aatggtgcac gttttgaaac
    29941 atacgtaata gctggtgaaa gaggtagtgg taaaatttgt ttaaacggtg cagcgtcaag
    30001 acttgttgaa gtaggcgatg tagtcataat catgacttat gcacagttga atgaagaaga
    30061 aattaaaaac catgcaccta aagtagcagt aatgaatgaa gacaatgtca ttattgaaat
    30121 gattcatgaa aaagaaaata cgatagtgtt ataaaattaa atagccatca ataataaaaa
    30181 gggccacacc ttggtttaag attccaagcg tgtggctttc tcttgttata cagtcctatc
    30241 actatgtcac ttttaatcaa aatcgaccgt aattgttgca tatcaattaa cgataggaat
    30301 gattaattgt gaatcatatt tactaccagt ataaatcatg tgcatgcctt tatttacggt
    30361 ttcttcatgt tcataacgtg ttttcatgcc aggggtacta ttaccaatta caatttcact
    30421 accttttaca acaacttcca atgtttcgcc ttgtttaaat agcgtgcctg aaggtaacaa
    30481 ttcgatttca acaggtacaa tctcatcttg tgacaacttt aattctgttt catgtttatg
    30541 ccaaggttgc gcgattgtgg atttttcttg atctaattca cgatgtgata cgcgtaacca
    30601 accagtagct acttgaccat tttcaatatg attaaaatca gggaagttaa cttcattacc
    30661 acgacgatct aactttttaa tacctgcaaa taaatccata tcgtctgagt ctgtagtgct
    30721 tacccatagt tttaagttca tgtttccaac taactcagta tctttgtcaa acgtatattt
    30781 aaaacttaca tcttgtcgtt tatcttcaga gtcatattgt gcgacatgcg cgctactgat
    30841 ctttgcatga ttcaatgtgt gattttcagc attcaaatac aatggtgtat attctgtgtt
    30901 aggtaaaggg aacgctgacg ctgatttgaa ttcaccttta taaaattgat ctctaacttc
    30961 ataaatgaca tgaggcgtat ctttccaatc gttattttct tctttaaggt aaaaatcaaa
    31021 gaatgatttt tggcgttcga gattttctct agcatagtaa ctttcccact ctttacgtcc
    31081 atgcacatat agccattttt cttctgatgc tgcttgttta aatccttcaa aagagccacg
    31141 gttgtgcaaa ccttgtgtag accaactagc acatgttagt agaggtgttt taatttgtga
    31201 taatggcact tgacgctgtt tccaaaaatc atcgaacaaa gggtgttctt gttgtgcttg
    31261 aatcaaatct tcgatatttg gattatctgt ccatctcgca aaaatacctt gagtccagaa
    31321 acgataaaag ccagtatccg ttatacctcc gtgaaaggct acttctctat acatatcatt
    31381 taagccttcc caaggaatca ttgcttttaa atgtggtgga tttaatgatg cgacccacca
    31441 ttgagtcacc gcaagataag aaacgccatt tgtcccgatg tttccattac tccatgactg
    31501 atttgctgcc cactcaatca cttcgtaata atcttccgct tctctttttg accatggaga
    31561 taagacgcct tttgatttgt cactaccgcg taatgcaact ttaactacaa cataatcatt
    31621 tggcacccaa aatcctgggt ctggtgattc ttcaggtgta aaactagatg tcggaatggc
    31681 gcctaatgtt ggccaaaggg cacccatatt tgtgatttta ggcttattat ctttaccgta
    31741 agtatctgca gacataacta cagggaattt gccatcttta tttggtctga aaatattaat
    31801 atataatttt tcgccatcgc gcatttccac agtgacatct ttttccataa tcatttcttg
    31861 atttccatat tgaacactgt cgacaacgat gtggttaata ccggctttca cttcattgac
    31921 atgagttaca gttaattttg gatttcctag taaatgttgg ttcattgtta tcaccttttt
    31981 ctgtttgttt gtatcaccta tattaaacag gtaaacattg tgtgccaatc atcaatgttg
    32041 gagatttgta ggataatgaa cataacattt ataattgttt ataatcattg gggaggactt
    32101 gatacaaatg actatagatc gacgtattag aaaatcacaa gttgcaatga gaaatgcatt
    32161 tattgaactt ttacatcaac accaattaga agagattact gtgcaacaaa ttgcagattt
    32221 agctgatgtt aaccgtagta cattttatac gcattattat gataagtatg atttgcttga
    32281 aaaattagag gaccagcagt tggaggaaat aagagacttc attcatgatg agaagttaaa
    32341 tggagcagtt aagttatcca cggataacat acatcaaatt atgaccgttt taatagaaat
    32401 gatagaaaaa gacatcgcat tttatcaatt gatgtttcgt atgggtaaag attcaaatat
    32461 ccacgaaaaa ctttatgatt taattatgtg tcatttgcaa cgttataaaa atgaaaatga
    32521 tcaaattaat ggtataccat tttcctattt catgagttat gtttcaggtg cgggattgtc
    32581 tttgataaga cattgggtag aagatccaaa ccgtatagac aaagttgact taataaggca
    32641 ttttaatacg attgtcaatc aagggccagc aacgataatt aaaaatatac aataaaaaaa
    32701 ggcaagccat gaattaagag tggcttgcct tgctaaataa cataagctta ttaaatgata
    32761 tctttatcaa taacgttatt gttggattgc agtatagtct tgaccatgta acatccaacg
    32821 aacaccgtat ttatcggtaa agatgcccat tttgccaccc cagaattggt cagcaaacgg
    32881 taattctatt tcaattgaag aatgatcttt aatttgctca tagaatgctt caactttatc
    32941 agcatcttcc ttattgttaa catcataatc aattaataat gatatgccat tattaatttt
    33001 gtcagcgcga ccaaaagaat cagaacatag cacttttaca ccaagcactt caaactcagc
    33061 atgcatagtt gcttcttgtg catcttcctt agtcatacca aaatgtgacg cttgttcttc
    33121 gccaacttct aaacgtttaa cgtcagttgc accaaatact tcttcgtaat atgcaagggc
    33181 ttcttttgaa ttttcaaaag cgatataagg gaataatgct gtcatgtaaa acacctcaat
    33241 gtattatttt tattactaac actatattac attaaaaaaa ggcaaaagta taacttttac
    33301 gatgaattga agtcaatctt ttgtaaaata cacgataaat tgtaagatga aattgattta
    33361 aatgaagtaa acatatatat atgacataca aaaaatgaaa atgacacaga gacgtatgtg
    33421 ttgtttagtg ttttaaaatt caagtttaaa aaatgctttt agatacataa gggattttca
    33481 aaatataaat gcattaaaat gaatttctaa cttttttgac tgacatttaa tatgaatcat
    33541 atataataga agttaatgat taaatgtggg aggttaaatc atgattaatg ctgaacctat
    33601 tatcagtaaa atgaaaaatc aaaaaattaa ttatgacaag gtgttgaaga aattaatcgg
    33661 gcagtgggaa agagaagcaa tcagacctaa aatcttattg catagttgtt gtgcaccttg
    33721 tagtacatat acattagagt ttttaacaca atatgcagac attgcaattt atttcgcgaa
    33781 ttcaaatatt catccgaaaa atgaatattt acgacgtgct aaagtgcaag aacaatttgt
    33841 ggaagatttt aatcgcaaga ctggtgcgaa tgtgaagtat attgaagcac cttatgaacc
    33901 gcataagttt gtgaaaatgg tgaaggataa agaattagct gatgaaaaag aaggcggctt
    33961 acgttgtacc gcttgttttg aaatgcgttt ggatattgta gcgaaagcag ctgtagaaca
    34021 tggctatgat tattttggca gtgcaatcac gttatcacct aaaaagaacg cacaattaat
    34081 caatgaactt ggtatggatg tccaaaaaat atacgatgtg aactatttgc caagtgattt
    34141 taagaaaagt aaaggttatg agcgttccat agaaatgtgt aatgactata atatctttag
    34201 gcagtgttat tgtggatgtg tctttgcagc gatgcaacaa ggtattgatt ttaaaacagt
    34261 taataaagaa gcgaaagcat ttttagaaca atatccagat taatcattgg aaataagata
    34321 aaaggcaggc gcatatacaa gccatgccct attaaaaatt atcaaataga aacccgagac
    34381 tttagtgaaa tgaatcatac taaaatctcg ggttatttat atcatcgtaa atattgtagc
    34441 tatcatgaat aactggattc tgagacataa aacaatgtct cactctaatt acatcatttt
    34501 atgaacaaat cttataattg ctaaaatgac aatgataagt gttgaaataa gtgaaaacac
    34561 tttaaacttt ttgtagtcat ttttcgtttc attttctcct gttttcatca tttcacgtag
    34621 ctcttgctcg atttctaaat caagatctgt tttacgataa ccattcaaac cttgacgttc
    34681 atcttcttca ggttgatttt gttgctgtgt gtctttgttg tcagaagtcg ctactgtttt
    34741 tttattatct gtttctttag tcataacaaa cgcctccgtt ataaaacgct atatttaatg
    34801 atatgtgata tttaagccat tattttgaaa aatggtgata aatatatctt cttgagttat
    34861 atcataattt taataagacg attcagcaag ttttaaagtg ttatttgact acgttggatt
    34921 aggcatctag tcctataata tcactgacat tgtcaaaatg atgatctttt aagtaacgtg
    34981 tgatgccttt gttcattttc ttagttaaac ctgggccttc aataacaagt gatgaataaa
    35041 tttgaataag tgacgcaccg tgacgcatca ttttgattgc atcttcagta ctgaatacgc
    35101 cgcctgtacc tataattaaa aattcaccat ttgtttgctg ataagcatac ttaatcaatt
    35161 ttaaattacg ttcaaataat ggacgaccac tcaaaccgcc ttcttcgact ttattagcag
    35221 aagttaaacc atctcgttgt cgcgttgtgt ttgctaagat gataccgtca aatgtctcag
    35281 taatcgctgg taatagtgct tttaagccat cgaaatccat atcagacgtt agttttaagt
    35341 aaattggcac tgttacatcg tgctgttttt taaatgttgt caaagcttga cataacattg
    35401 aaaattcatc tttatcatgg aaattttgaa ggttttcagt atttggagaa ctgatattga
    35461 ctgtgaaaaa tgaaacgtcg tgtttaaacg tatcaatgac ttttatataa tcttgataac
    35521 gcgcttcata aggtgtcatt ttattcacac caacattgat accaacaggt acttgataag
    35581 catttttacg caaatgactg agtgctttgt tcataccaat attattgaag cccatacgat
    35641 ttatcaaggc atcatcttct aataatctga acatgcgtgg ttgagggtta cccggttgag
    35701 gtttaggtgt gataccacct aattctaaag caccgaatcc aaggtgttcc aatgcttttg
    35761 gtacttcgca agatttgtcg aaaccagctg ctaagccaat tggattgtcg tacgtattac
    35821 cttgtatcgt ttgtgataac gttggattct tataagtaaa tagtttatcg acgactggga
    35881 ataaaactgg aaatttttgt aacgttttta atgcatcgat agttagtccg tgtgcttttt
    35941 cgggttcgat tttgaataag aaaggtttaa ttaatttgta catgagtatg ctcctatttc
    36001 attatatttg aggcttacta tcctcaactt aatatatgtg aaatatattc ttttaataga
    36061 ctagcatttc cctacataat ttcctagtta aaactaaaaa gttttgaaaa ttgacgcaag
    36121 taccacaaat aaaattgaga ttaaatcatc ctaattaggc aatataatag tataaagtaa
    36181 gcgtaatgga aggtgtttgt atgaatgaac aatggttaga gcatttacct ttaaaagata
    36241 ttaaagagat ttcaccagtg agtggtggcg atgtaaacga agcatatcga gtcgaaacgg
    36301 atacggatac atttttctta cttgtccaac gtggacgtaa agaatcattt tatgctgcag
    36361 aaattgcagg tttaaatgaa tttgaacgtg caggtatcac ggcacctaga gtaattgcaa
    36421 gtggcgaggt taacggtgat gcgtatttag tgatgacgta tttagaagaa ggggcttcag
    36481 ggagtcaacg ccaattaggg caactcgtag ctcaattaca cagtcagcaa caagaagaag
    36541 gcaaatttgg cttctcatta ccttatgaag gtggcgatat ttcttttgat aatcattggc
    36601 aagacgattg gtgtaccatt tttgtcgaca agcgcatgga tcatttgaaa gatgagttgc
    36661 taaatagagg gctatgggat gccaacgata tcaaagtata tgacaaagtg cgacgtcaaa
    36721 ttgtggcgga attagaaaag catcaaagta aaccatcttt attacatggt gacctatggg
    36781 gtggcaatta tatgttctta caagatggtc gtccggcgtt atttgatcca gcaccattat
    36841 atggtgacag agaatttgat atcggtatta caacggtatt tggtggtttt acgagcgaat
    36901 tttatgatgc gtataataaa cattatccac tcgcaaaagg tgcatcctat agacttgaat
    36961 tttatcgttt atatttattg atggtacatt tattgaaatt tggggagatg taccgtgata
    37021 gtgttacgca ttctatggat aagattttac aagatacaac agattagtta ttacgttaga
    37081 ttaagataat aatatgcaca gataatttta taatgagaag gccttcagct gcctcaataa
    37141 aaatatttgt gcttttttat tgttggaaaa taaaatttta atctctattg ttaatttcta
    37201 taatacaaaa caagattgag ttgcaataaa agtgattttc taactttttg ttcaataaaa
    37261 ttctaggaat gatacatatt tattgataca ataattttga atataatcac aaaacaatat
    37321 ttaagtataa ttgaatgttt gaatatcata tattgataca gtttctaata attttaaaat
    37381 aatttaaatg ggaagaggtg taaatgatga gtacagttca aagtgatatt tttaagacta
    37441 atagtgcatc atcatctatt aaaagcgctg ttgaaacatg taataatgtg tcgaaaccgg
    37501 ataaagatga aagtacaaca gtaagtggaa ataataatgc tcatagtgtg atagatgatt
    37561 tgattagtaa gaatcaatct gttgctgaag caatacgaac tgcgagcgat aatatacaaa
    37621 aagttggtga ggcttttgac caaactgacg taatgattgg taatgaaatt ggtaaaaatt
    37681 aaaacgtggt gaaatgatgt cgaataaact tgatggaatc aataagatga tcacagcgaa
    37741 acataagcaa atggatgact tatatgatga aaagcaagag gttaaagcat tgatagatga
    37801 aagcgatgcg cttaatcatt cgatagaaca attatatcaa catttaggtg agcgttatta
    37861 tagtagcaat atggctagtc gtatggaaca gttccgcgat gaatttcatt ttgcgaaacg
    37921 acgttcaacg gaagcgttat acgagcagca acagcaaatt caacatgaca ttcgtaaagc
    37981 ggaagaagag atgattgact tggaaatgcg aagaaatatc gaaatcgaga tggtgacaaa
    38041 ggaggaaaat aaatggaaac aataggaagc attatttatt taaaagaagg ttcgcaaaag
    38101 ttaatgatta ttaatagagg accaattgta gaaattgaaa atcaaaagta tatgtttgac
    38161 tattctgcat gtaaatatcc gattggtgtt gtggaagatg aaatttatta ttttaacgaa
    38221 gaaaatatag attcagttat ttttaaaggt tattctgatc aagatgaggt tagatttcaa
    38281 gagttgtttg ataatatgaa acaaaatttg gatagtgaaa tacaacgtgg agaagttacg
    38341 ccacaataaa gaaatacttt atatttattg gagggacgcc gaaataaatt ttgtaaaaat
    38401 atcatttctg tctcaccgcc aaaatgttat ctccaagtac tgaggctgga tgataccatg
    38461 aaatgtcatt cataatcatt tgaactaacc gttgcatcct taaatttaat aaaaaagaca
    38521 cataccaact tccgaaatgt agatgaattc tctacaatag cggaagtttt tcttttaata
    38581 ttgaaatttc tcaaggatag gtctatactt tataaatcgt aattattacg atttataatc
    38641 aaaaacaata acttgaaata gatcattgag ggagtgttaa tatgcaacat cataaagtgg
    38701 ctattatcgg tgccggtgct gcaggtatag gtatggccat taccttaaaa gatttcggta
    38761 taacagatgt cattatttta gaaaaaggaa cagtaggaca ttcatttaaa cattggccga
    38821 aatcgacccg tacgattacg ccatcattta cgtctaatgg atttggcatg cctgatatga
    38881 atgcaatttc catggatact tcaccagcat ttacttttaa tgaagaacat atttccggag
    38941 aaacatatgc tgaatattta caagtggttg ccaaccatta cgagctgaat atctttgaaa
    39001 atacagttgt cacaaatata tctgtagatg atgcatatta tacgattgca acgacaacag
    39061 agacatatca cgcggattat atctttgtcg caacaggtga ttataatttc cctaaaaagc
    39121 catttaaata tggtattcat tatagtgaaa ttgaagactt tgataacttt aataaggggc
    39181 aatatgtggt tatcggaggt aatgaaagtg gctttgatgc tgcatatcaa cttgcaaaaa
    39241 atggctctga catcgcactt tatactagca caaccggttt aaatgatccg gatgctgatc
    39301 ctagtgttag attgtcacct tatacacgtc agcgactagg taatgtcatt aagcaaggag
    39361 ctcgcattga aatgaatgtg cattatacag ttaaagatat tgattttaac gatggacagt
    39421 atcatatcag ttttgatagc ggacaaagtg tgcttacgcc tcatgaacca atagtagcaa
    39481 ctggctttga tgcaacaaaa aatccaatcg ttcaacaatt atttgcgaca acaaatcaag
    39541 atattaaatt aacaacacat gatgaatcga cacgttatcc gaatattttt atgattggtg
    39601 caacagttga aaatgataat gccaaattat gctatatcta taaattcaga gcgcgatttg
    39661 cagtacttgc acatctttta acacagcggg aaggcttacc agctaaacaa gaagtcattg
    39721 aaaattacca aaaaaatcaa atgtatttag atgattattc atgttgtgaa gtgtcatgca
    39781 catgttagaa gtgaaatatg atatgagaac ggggcattat acgcccatac ctaatgaacc
    39841 tcattatttg gttattagcc atgcggataa acttaccgca acagaaaaag cgaaattaag
    39901 attattaatc ataaaacaga aattagatat ttcattggca gaaagtgtag tttcttcgcc
    39961 tatagcgagc gaacatgtga tagaacaatt gacactattt caacatgaac gacgacattt
    40021 aagacctaaa ataagtgcga catttttagc ctggttgttg atatttttaa tgtttgcatt
    40081 gccaatcggt atcgcttatc aattttcaga ttggtttcaa aatcagtatg tatcagcatg
    40141 gatagaatat ttaactcaaa caacattgct caatcacgat atattacagc atatattatt
    40201 tggtgattat ggtgtgctat cacttggaac atattcgctc gtatgggcat tgccggtcgt
    40261 aatattgatt agcttatcaa ctgctgtaat tgatcaaaca ggatttaaat catggatgat
    40321 atgggcaatt gaaccttcaa tgttacggat aggattacaa ggtaatgata tcgtgccact
    40381 attagaagga tttggatgta atgcagcagc tatttcacaa gcagcacacc aatgccatac
    40441 ctgcacgaag acacagtgta tgagtttaat aagctttggt agttcttgta gttatcaaat
    40501 aggtgcgaca ttatctattt ttagtgtagc tgaaaagtca tggctattta tgccgtactt
    40561 aatattagta cttttaggtg gcatcttaca taataggata tggttgaaaa agaatgatca
    40621 acaacttagc gttccgctac cttatgatag gcaattacat atgccaaata tacgtcaaat
    40681 gttgctacaa atgtggcaaa atatacaaat gtttatcgtt caagcgctac ctatttttat
    40741 cacaatctgt cttattgtta gtattttatc actaacgcca attttgaatg ttttatcaca
    40801 aatatttaca cctatattat cgttattagg catctcgtca gaattgtcac cagggatttt
    40861 attttcaatg attcgaaaag acggcatgct cttgtttaat ttgcatcaag gcgccttatt
    40921 acaaggaatg acagcaacac agttactact acttgtgttt tttagttcaa catttacagc
    40981 gtgctcggtc acaatgacga tgcttttgaa acatttaggt ggtcagtcag cactaaaatt
    41041 aattggaaag caaatggtga catcattgtc tttagttatt ggtgtaggca tcattgttaa
    41101 aatagtaatg ctgattattt aaaaaagaaa tgaactataa ctgaatatag agtcatgtca
    41161 gtcaatagga aagctatctt ggaatatgct attcatatga agtataagag gagagtcgca
    41221 gatgaaaata gttattatag gtgggttttt aggtggcggt aaaacgactg tcttaaatca
    41281 tttgctcgct gaatcattaa aggagtcgct gaaaccggca gttatcatga atgaatttgg
    41341 caaaatgagt gttgatggtg ccttagtatc tgaagacata cctttaagtg aactgacaga
    41401 ggggtgtatc tgttgtgcaa tgaaagcaga tgtatcagaa cagttacatc aattatattt
    41461 aaaagagcaa ccagacattg tatttattga atgtagtggg attgcagaac cagtctctgt
    41521 cttagatgct tgtttaacgc ccattttagc tccgtttaca acaattacac atatgattgg
    41581 tgttgtagat gcaagcatgt ataaacacat aaaatcattt ccaaaagaca tccaaggctt
    41641 attttatgag caattagcat attgttctgt cttatttgtt aataaaatag attcagcaga
    41701 tgttgaaacg acgagcaaac tattgaaaga tttagaagtt attaacccag aggccgatat
    41761 acaagttggt atgcatggca gcgtcacttt gccaatatca gttagccaaa tgacagcaac
    41821 ttctgacaat aaacatgaat cattacacaa aacaataaat catcaatttg tgcaatcacc
    41881 agccaaatgt actaaagcac agtttataaa acgtttagca tgccttccga ctcatattta
    41941 taggttgaaa gggtttatga catttgaaga caccgcacat acgtatctca ttcaatttac
    42001 acaaggacaa tatgaattaa cacctgtagc attttcaaaa aaagtgccag aatatttggt
    42061 gcttatcgga aagggtattt caaaagaaga ctatcaatgt ttggaacagt agtgttttca
    42121 gtggaagaga atggttaaca tgccttcatg tataataacg agttgatttg aacgtttaag
    42181 cgtaaataaa aataagcttg gtcagccatc aaatataatt tgaaaactgt ccaagctgtt
    42241 ttattagaga acgatcaatt aaccccacat atttaataat acatcagcaa agccttcagg
    42301 tttttgaata taacctaagt gaccgcctgg aatatctaca ataggtatgc cagtttcttt
    42361 atttatataa aagttaacat cttgtgggaa ggagcctcta gaatctgtcc catttagtaa
    42421 ggtgatttta tcgctgtatt ttgtgaaatc atccaaagta atatctgaat gcgtatattg
    42481 tctaatttca aattttgacc agaacatcgt acgtttgtac tgttctatac gtccttcttc
    42541 agtatcagca ggttgagaca tcatttttgc atcaattggt gcgatattta atgtttcacc
    42601 aaatgttttc ataccttttt ctaagccttc tgttaaaatt tgatgcacaa tgtcatcatt
    42661 tttatctttc caataagtac tgtctggtaa aaatgtatta attggtggtt catgaaatgc
    42721 aatcttttta acgacttcag ggtaatcttt taacacatgc atcgcaacga ttgaacctga
    42781 acttgaacct aatatataga caggttcatc acttaatgac tttgcaagtt cggcaatgtc
    42841 ctgtgcgtcg cgtttgacac gataatcact gtcagggtta gaagcggaat cagggagtgg
    42901 ttcagctaac tcgctttctc cataatcacg acgatcaacg gctacaacag taaaatggtc
    42961 ttttaactgt tctgcaagag gtagaaaaat gtctccggta ccgtttgcac caggaataaa
    43021 gatgagcacg ggtccttgtc cgacttggtg gtagcgtaat ttagcgcctt gtaattctaa
    43081 agtttccata ttcaatgacc tccatttgtt aattgttaga tgataaacct aataatttag
    43141 caccatttgt ataacttatt ttctcttttt cttcatctgt taaacccagt tcatctaaaa
    43201 atacacctaa tttttcaggc tcaatatatg gataatcagc agcataaaga attctatcaa
    43261 tacctgcttc tttcttgact aaatcaaact gtggcttcgt taacatacca ctcggtgtga
    43321 tataaaaatt atttttaaag taatagctta cagggtggtt caaatgttca gcgaataaag
    43381 cttcatccat acgttctaag aagaatggga taaactcacc ccaatgtcca ataatcatat
    43441 ttaactttgg ataacgatca aaaatgccgg ataatactag atgtattgca tgaataccaa
    43501 catcaatgtg ccaaccataa ccaaaacaag caaatgttgc cgcggttact tcaggataat
    43561 ttcctttata gtatgattga taaatgtcac tgttaactgg cgcgggatgt agataaatcg
    43621 gtacgcctaa attttcagct gttttgaaaa taatgtcata tttgtcttga tcaagaaaac
    43681 catcttgtgc acgtcccata atgagcgcac ctttgaatcc taaatcattg atgcaacgtt
    43741 cgaattctcg cgctgcggct tcaggctcat tgataggtaa agttgcaaag cctacaaagc
    43801 gattgggata ttgtgcaata tagtttgcca attgatcatt tgctttttga cataattcaa
    43861 tggctttctg accaactaaa tttgaaggag acccatttcc ataagataag acttgaattt
    43921 gaacgtcttg attattcata aattggatac gttcatcatg atgtgataat tcgtcggcat
    43981 ttgtaaaacc tgtctttttt tcaaggcctt ctaacattac tttcatcggc acacctttag
    44041 gatctgccga tatcgcattc atcgtttctt tttgaatatc ttcaatgaca taatgttctt
    44101 caaacgtaat acttttcatt tgattcgcct ccatattgtt ttgcatgtat attgcatcta
    44161 ttgcagaagc attttttatt tacctctaaa ttcaatattt gtaacataaa tttggattac
    44221 caaggcattt caccattccc attaataaat gtacctgtcg ggccatctgc atcaatggtt
    44281 gctaattgaa tgattggctt gatcccttca gaaacatgtt ttgaattatt actaaaatca
    44341 ccaactaaat cagtgtttgt agcacctgga tcagcagcat tgatttgcat atttggtaat
    44401 cctttagcgt attgtaatgt tagcattgtt actgccgatt tagacgaaca ataagctaat
    44461 gaattcactt tagattcagc tgtttcgggg tttgtaacca ttccaaatga acctaaacca
    44521 cttgatacgt tgacgacaac aggttgttca gatttttcta agagagggac gaatgtgttc
    44581 atcattcgaa cgataccaaa tacattcgtt tgatatactt cttcaacgtc acgaggtgtc
    44641 aatttagaag gtgttgaaaa ttgaccagat atacctgcat tgttaatgag gatatcaaga
    44701 cgaccttctt tttcagcaat catgttataa gcatttttga cagagtagtc acttgtaaca
    44761 tctaactgta cataatgaac acctaatttt tgagatgctt gttgtcctct tacgtcatta
    44821 cgagaaccta tatacacttt gtagcccaat gctttaagtg cctccgcact tgcatagcct
    44881 aatcctttat taccacctgt gattaacaca attttagtca ttacgttcca cctcatctaa
    44941 ataaatgttt aataaataat ttctatacgc ttcaattgaa atattgcgat gttctatttg
    45001 gaaatcaaat acacgtgttg ataatgcttg caacagcata tctgttttga acttcgtgta
    45061 aatgtcgtca tcgcttttaa acaagtcata ataaaaataa aataattctt gataaaatgc
    45121 gctttggtaa aaacgtaatt tattgttgcc tgcttcaata cattgcagta gtgccttatg
    45181 gtcgatttta aattgtaaaa gataatctaa cgacacttgc ataacctcat aattagaatg
    45241 atagtcatct ttaatttgct taaaatgagt gataaaaata tcaaggtctc tttgtatgac
    45301 gtagtagcat aaatcgcttt tatctttaaa atgtcgatac aatgtcccca taccgatacc
    45361 tagttcttta gcaatacgat tcatactaat gttttcaacg ccttcttcat caaaaagttt
    45421 gtgcgctatt tcttcaattc gttgcctatt ctcttttgca tcttttcgca tgattccacc
    45481 tacttatagt tctctaaaat tgacaaacgg ataactctcc gtttattata aaacgtgtta
    45541 agaaagttag caatgaattt gcaataacta ttaaatatca taaaagaaaa gagtgttgcg
    45601 tatgtcttta aataaagagc aaagacgtat tacagctgaa gaattgcaag cacattttga
    45661 agaatctacc ttatcggttc aaatgattgc aggaaaactg aatgttacta cagaagatgt
    45721 ggaaaaagca ttagctatga aagcgccact aggcattttt agtcatcaat tacaacgatt
    45781 tattcattta gtatgggatg tcagagatgt aataaatgac aatattaagg aaaatggaca
    45841 aacaccagaa ccatatacgt atttaaaagg tgaaaaagag gactattggt ttttaagata
    45901 aaaggggagt ggatctaaaa tgaaagatat tttagtaata ggcgcaacag ggaagcaagg
    45961 taatgcagtt gtaaaacagt tgttagagga tggatggtat gtaagtgcat taactagaaa
    46021 taagaataat cgtaaacttt cagacatagg acatccgcat ttatcaatcg ttgaaggtga
    46081 tctgagtgat agtgttagtt tgcaatcagc gatgaaaggg aagtatggtt tatacagtat
    46141 tcagccaatc gttaaagatg atgttagcga agaattaaga caaggtatga agataattga
    46201 aatagctgag caagaaaata ttcaacatat tgtgtatagc actgcgggag gggttaatcg
    46261 aaatcgaaca ggcccacact ttgaagtact agcaaaaata gaaaatagat taatggaatc
    46321 taatataaat gcaacagtta tcaaaccatc attctttatg gataattttt tacgtattgc
    46381 taaagtcgaa gatgaacgta ttacattacc agaatttatt aatccgaata taaaattcac
    46441 aatgatttct tctattgata tagctaaaat tgcatcgtat gtttttgaac atccacaaag
    46501 ctttacgcat caatcaatag agattggttc cgatgaagtt acattaagtg aagcagcaac
    46561 aatttttagt gaagtgacag gtaaatcgac tgttatagaa ggagaatttg ttagtggtgt
    46621 tgcagaaaag caatggttgg aagaaaaagg ttatgaagta gactttgaat taatggctga
    46681 aataaatcca acaagattat cattaagtga ttggcttaaa gtccaagact ataataagta
    46741 atgaatgggg tgcaatcatg attcaatcaa tgtggtttaa tttgcatgtg caagatttag
    46801 aaaagagcgc acagttttat aaagcgttag gatttaaaat aaatagaaac ccacaaatgt
    46861 tagataaaat ggtaggcatt caaataggtc aaacaaccgt aattttaata gaaaacaagc
    46921 attttcaaaa tgtaagtcag caaagcctta acactgaacc aaatgaagtg atgatttctt
    46981 taggtgtgaa tacaaatgaa gaagttgacc agttggtgaa taaagtgaaa gaagcgggtg
    47041 gcacagtcgt tcaagaacca acagtaagcc aagggtttta tggtgcaatg ttcaaagatc
    47101 ttgatggaca ccattttaat tttttagtct gctaaaatga aattctcaaa agaataaata
    47161 aaaagcccat acagagatga agcgtaatgt gcgcatgtcg taagagacat ggtgcgacac
    47221 attagtgtct tcaatcagta tgggctttgt ttatgaggtc agttggcacc ttaagtgcaa
    47281 tacctcgtga gcaaatatta attttgaata taaccgtatt gttggcgaag atcataaact
    47341 ttatctggtg tgacatcctg accgattgga tcaaccactt ttattgtgga aaatgttgtt
    47401 aatacttgtc cccggatcat gcttaagtag tcttggcgca atgcatgttg ttcttgtttt
    47461 tcagctacag taagtggttg tactttttct ttatttgcaa gttcattaat tctatctaaa
    47521 attttcatat tgtcatatcc tccttagaat caagtgttga tatattttga gattatttct
    47581 atcaggtaat ttatcaacac attcagtaat gtttttagtt tctgggtctt ttgaccagca
    47641 gatttttgga attaaaataa ggggtttgag acacgatttt aatagtagta cgacggcaaa
    47701 gtgatgttat tgaagtgccg tttgatatac atgtctgcaa tgttgtttta taagacagga
    47761 tgacgtaact gagcaaagaa atgagcattg tcgaatgaca tcgtgtgtct tattaaaatt
    47821 gcaatggcca tcgcataggt atattcacag tcgattttct tgatttctac attatcataa
    47881 tacgtagtgt aaaagttata cgacagtcga tagtcgattc gcttattacc acgagcaatt
    47941 attatgtgct gcttcaactt cgacttcaat ttacttgtaa tgaatgtcat tagactctga
    48001 ctattctgat taatatttaa tctgttatgc gtgtgttcaa acccttgagg ttcatctgtc
    48061 tcgctcacgc caatccctcc tatcttagta tttaaacaac gcgtatcaac tatgcgacat
    48121 gtgttgttta gacatactat actactatgt cgtacgtttg ccaataaaca cggacatatc
    48181 atttgttgcc taaacaacat attgcctgaa gtgttgttta agtacgcaaa aacaaaaatg
    48241 cttgtcacta aaatctatat gttttaatga caagcttatt ttcattcagt taacgactgc
    48301 caatatggct ttgtagagta taggacgttg atttatgtcc tagacacggc taatcaatcg
    48361 taatacgtta aattttattg tgtgtacaag tatgaaccta agtcacatag tgatttcaag
    48421 atgcatgact aaataccaag ttttattaaa aatgtgctat atgatgaatt aaaaatggaa
    48481 ggttgcgatc gataaacaca tcggctgttt cttgactcgt gttaaaccaa tgtaatgcta
    48541 agcctgatat accagctgct aaaaaactcc cactatttac aatgatatct cgttgatgct
    48601 ctgggtagct gttttctaac ataatcgcaa aaatatcttc taattcatta ttaatacgtt
    48661 gatgtgcttt gttgcaaaat gcttcactat ttaattcgca agactttgca gcatctttga
    48721 tataactcgc aattgaaatg aaaatattac gcagtacctt ttcattaatg acattagaaa
    48781 tgctcaagtt atcattcaag tcttttaaaa tggttacaga caatgtgtag tctaggaggt
    48841 cttctttatc agtgaaatga gcgtaaaatg ttgcacgatt taacgtagca atatcagtga
    48901 tatctttaac tgtaatttga gacaatttct tttctctaga aattttaaga aaagcatcga
    48961 caagcaattg tttggttcta acgattcttg gatctatgga tttactttcc gtcatttgtt
    49021 cactacctaa catctgttgt tttaacactt attatcagta tcttctatta tgttgtcaat
    49081 tgtgttgatg atatgtaagt gctgatacgc tcctaacaaa taatgtatct ttttggtaag
    49141 catttgaatg tctttcttct attatattaa tggattttaa aaatgaagat aaaataaaaa
    49201 gtgtattgta acaatatgat tgaaaaattg ccaatttctt tatctaaaaa ttcagaattt
    49261 tgtgatatac tatttttcat agtcttgagt tatagatgat actgctagcc taaatataac
    49321 aacatcggca tgcacatctt agaatttaag ctagtggcag cttataactt aaggctcttt
    49381 attatttgca taaagatgta tatacataaa tggaaggatg atattatgac atcaattaaa
    49441 acaattacac ccaaagattt tatatttaga gttttatcag gtgtcgccat aggtattgtt
    49501 gcgggtttag ttcctaatgc gatacttgga gaaattttta aatactttat ggattatcat
    49561 ccgattttta aaacgttatt aggcgttgtc gttgcaattc aatttacagt acctgctttg
    49621 attggtgcat tggttgccat gaaatttgac ctatcgccat tagcaattgc tgttgtggca
    49681 agtgcagcat atgtaggaag cggtgcagcg cagtttaaaa atggtgcttg gatgattacg
    49741 ggtattggtg atttaattaa tacaatgatt acagcagcga ttgcagtgtt gttcatttta
    49801 cttattcaac atcgtgtagg tagtatggct ttgattgtat tcccaacagt tgttggcggt
    49861 atttcaggtg caattggtat tttaatattg ccgtatacga aaatgattac aacagccatc
    49921 ggtaatatgg ttaatggttt tacagaatta caaccaattg tcatgagtat tttgatttca
    49981 atggtattta gtcttattat tatttcgcca ctatcaactg tggccattgc ctttgcgata
    50041 ggtattacag gtttagcagc tggatcagcg tcaattggta tttccgcgac tgaagcagta
    50101 ttgataattg gtacaagtaa agtaaatcgt ttaggggtgc cgttatccgt cttctttgga
    50161 ggcgttaaaa tgatgatacc taatatggtg aaatatccaa ttcttatgtt accaatattg
    50221 acgacagcca ttgtatcagg attggtatca gcattagtag gtattcatgg aacgaaagag
    50281 tcagctggat ttggttttat aggaatggta ggaccgatta atgcatttaa atttatggag
    50341 gttgattctg cttggttgag tgtcttacta attgtagtag cgttctttgt tgtaccattt
    50401 gtaacagctt ggcttgcaga tatcatttat cgtaaagtgt ttcgtttata tactaatgat
    50461 atttttaaat tcatgggata aggacaatat gttttttgaa aatattagac caaaaattat
    50521 ttaatgttat tgtgaattgc gagaataaag tttagcaata taattgagct gcagtttatt
    50581 tgtgcttata aaagataagt caatacacaa aagatatgtg ttttaaaact ttgaatatat
    50641 aacaataata gcaatcaata catgtgaggg aatctatgta ttgattgctt tttaattgtg
    50701 tttaattaat caatttaagt tatatttgat gacggtaatg ttacaggcgt gtaacaaaaa
    50761 tacaatctgt aatattacga aagctgtaat agttgagcta aatatagtaa aaaacttatg
    50821 catttatgct tatttcaagg attgattaga ttctaaaaga aacatatttc acattacagt
    50881 ataaaacaac catatttcaa cattacaaat aacacctgat attgtaatgt tttgtaaaga
    50941 aagtgtaatt tacttgctac ttttttatga tatagtgagt accgttgaaa gaaataaaag
    51001 acaatttaaa cttgcactac gacattcaaa ttcaaaatat aattactaaa aacataaact
    51061 tacaattatt actggaggat tttttactat gaaaaagaca attatggcat catcattagc
    51121 agtggcatta ggtgtaacag gttacgcagc aggtacagga catcaagcac acgctgctga
    51181 agtaaacgtt gatcaagcac acttagttga cttagcgcat aatcatcaag atcaattaaa
    51241 tgcagctcca atcaaagatg gtgcatatga catccacttt gtaaaagatg gtttccaata
    51301 taactttact tcaaatggta ctacatggtc atggagctat gaagcagcta atggtcaaac
    51361 tgctggtttc tcaaacgttg caggtgcaga ctacactact tcatacaacc aaggttcaaa
    51421 tgtacaatca gtaagctaca atgcacaatc aagtaactca aacgttgaag ctgtttcagc
    51481 tccaacttac cataactaca gcacttcaac tacttcaagt tcagtgagat taagcaatgg
    51541 taatactgca ggtgctactg gttcatcagc agctcaaatc atggctcaac gtactggtgt
    51601 tccagcttct acatgggctg caatcatcgc tcgtgaatca aatggtcaag taaatgctta
    51661 caacccatca ggtgcttcag gtttattcca aactatgcca ggttggggcc caacaaacac
    51721 tgttgaccaa caaatcaacg cagctgttaa agcatacaaa gcacaaggtt taggtgcttg
    51781 gggattctaa ttccttcata ataaataaaa gtaatgttta aacattaaat atatagctct
    51841 tcccatagaa attggggaga gtttttttat tagaaattaa agtctgattc ataggttagg
    51901 atgttatttt attattcctg acgattttaa tgtcatgata gaagaattaa tgttactatt
    51961 aaattagaat aaggcgaaat atacaatata ccatgattct ttttacacaa atacttcaat
    52021 atggagtgag tatttgtcat ttcatatggt taaatttgaa agagaggtgg aaagatatca
    52081 ccgtgtatat attttaataa cagtgcgcgg taatttattc attatagaaa ttttgaaaaa
    52141 gtaaattccg aatagcatat cttaatgatt ctgttacagt aagatgcata aaatataaaa
    52201 cgaacctttt ttgtaaaaat ataatgaaaa taacagttat tttgctaaaa attcaagaat
    52261 ctatgataat ctatgttatg tagtaaaatc gacaaattta tatgtggttt gcagtaatga
    52321 aattaactgt aataattgtt ttctacttaa taaaaaattt aaaactatct taattaaggg
    52381 ttaaatcaat taatgaaaat cagtgcaata ttttattttc aaaaatattg tgatatgcta
    52441 caaaatagag aaaaataaat ggggcgttta ttaacatgga tacaaaagac tttaaacgtt
    52501 tagaaaaaat gtattccccg cgatacttac ccggattaga tggattgagg gcattcgcag
    52561 ttataggaat cattatttat cacttgaatg cacaatggtt aagtgggggc tttttaggag
    52621 tagatacatt cttcgttatt tcaggttatt taataacaag tttgttgata agtgagtatt
    52681 atcggacgca aaaaatcgat ttgctagagt tttggaagcg acgattgaaa cgactcattc
    52741 cggcagtgtt atttttaatc tgtgtcgtgc ttacgttcac cttgatattt aaaccggaat
    52801 tgattataca aatgaaacga gatgctattg cagctatatt ctatgtttca aactggtggt
    52861 acatttcaca gaatgtagat tattttaacc aatttgctat tgaaccactc aaacatttat
    52921 ggtctttagc cattgaagaa caattttact tgcttttccc attggttatc acgttcttat
    52981 tacatagatt taaaccgaga aatattattc aaacgctatt tattgtatcg ttgatttctt
    53041 taggacttat gatagtgatt catttcatca ctggagataa ttcacgtgtg tattttggga
    53101 cagatacacg actgcaaact ttattgcttg gttgtatatt agcatttatt tggcctccgt
    53161 ttgctttgaa aaaagatatt tctaaaaaga ttgtcgtatc attagatatt atagggatat
    53221 ctggttttgc ggttctaatg actttgttct ttatagttgg agaccaagat caatggatct
    53281 ataatggagg attttacatt atatcatttg caactttatt cattattgcg attgcggtac
    53341 atccttctag tttatttgct aaatttttaa gtatgaaacc tttactaatt ataggtaaac
    53401 gatcatatag cttatattta tggcattatc ctatcattgt ttttgtgaac agttattacg
    53461 tacaaggaca aataccggta tacgtttata ttatagaaat tttgttaaca gcgttaatgg
    53521 ctgaaatttc gtatcgcttt attgaaacac ctatacgtaa aaaaggattt aaagcttttg
    53581 catttttacc taaaaagaag agtcaatttg ctagaactgt gttagttatc ttattattga
    53641 ttccatctat catcgttctc agtgggcaat ttgatgcgct tggtaaacaa catgaagctg
    53701 agaagaaaga gaagaagacg gaatttaaaa caacgaagaa aaaggtcgtt aaaaaagata
    53761 agcaagagga taagcagacg gcgaatagta aagaggatat taaaaagtca tcaccactat
    53821 taattggtga ctcggttatg gtggatattg gtaatgtctt tactaagaaa ataccaaatg
    53881 cacaaattga tggtaaagtt ggacggcaac tcgttgatgc tacaccaatt gtgaaatcgc
    53941 aatataaaga ctatgctaaa aaaggtcaaa aagttgtagt agagcttggt acaaatgggg
    54001 catttacgaa agatcaatta aatgaactat tggatagttt tggaaaagca gacatatatt
    54061 tagtttctat tagagtacct agagattatg aaggtagaat aaataaatta atttatgagg
    54121 cagctgaaaa gcgctctaat gtacatctag tcgattggta taaagcttct gcaggtcatc
    54181 cggagtactt tgcatatgac ggtattcact tagaatatgc aggtagtaaa gcgctgactg
    54241 atttgattgt aaaaacgatg gaaacacatg ctacaaataa gaaataattt gatgcactaa
    54301 acttttgaaa tattacatta cttctgatat ttattatcaa aaaggatgta tttcattaaa
    54361 agtttagtgc ttttttattt tcaaatccca tgttaacggt gcagaaaaag tgttgtaaac
    54421 attctaattg gtatattaca ttcaatgatg ctttgttagg aacagattac attatgataa
    54481 caaagaccac aaggtaccta atctctgtta tagtttgttt tgtcgcaaaa ctataaaagt
    54541 tataattatt tgcatactaa aaaaataaaa aatacaaaat ttaaaataat tgagtcgcta
    54601 atgactatat gtatagagtg ttttgattat tgggaggata tttaattatg aaaaaaatcg
    54661 ttacagctac aatcgctaca gcaggacttg ccactatcgc atttgcagga catgatgcac
    54721 aagccgcaga acaaaataac aatggatata attctaatga cgctcaatca tacagctata
    54781 cgtatacaat tgatgcacaa ggtaattatc attacacttg gacaggaaat tggaatccaa
    54841 gtcaattaac gcaaaacaac acatactatt acaataacta caatacttat agttataaca
    54901 atgcatctta caataactac tataatcatt catatcaata caataactat acaaacaata
    54961 gccaaacagc aacaaataac tattacactg gtggttcagg tgcaagttat agcacaacaa
    55021 gcaataatgt tcatgtgact acaactgcag cgccatcttc aaatggtcgt tcaatttcta
    55081 atggttatgc atcaggaagt aacttatata cttcaggaca atgtacttat tatgtatttg
    55141 atcgtgttgg tgggaaaatt ggttcaacat ggggcaatgc tagtaattgg gctagcgcag
    55201 ctgcatcatc tggctataca gtgaacaata caccaaaagc tggtgctatc atgcaaacaa
    55261 cacaaggcta ttacggtcat gttgcttacg ttgaaggcgt taacagcaac ggttctgttc
    55321 gtgtttcaga aatgaactat ggacatggtg ctggtgtggt tacatctcgt acaatttcag
    55381 caaaccaagc aggttcatat aatttcattc attaatcaaa tgtaaatcaa atgacgtcaa
    55441 tattctctaa catgagagta ttggcgtttt tgttttatat aaatataaat ataaatgaga
    55501 gcggtttatt cactgatctt tagggaacta agtaataaaa tgataattta tactatgtca
    55561 gtatgattga gagtgattca atttagatga aaatcatgaa aaaatatatc aaaacagcat
    55621 ttttctgtag tatgtattgg ttaattgttc aactaaatat agcaaattta ggtacaagaa
    55681 ttcctgacaa gtattttcgt cagaagcata taatatttaa atcatttaac tttgagaagc
    55741 atggaaaatt ttggaacaaa tggttttacg taagaaaatg gaagcataag attttagatg
    55801 gtcataagct taatcgaaat atatatgatc agcgtcattt aatgacaatc aatattgatg
    55861 aaatcgaaaa aatgattata gagacaaaga gggcagagtt gattcattgg atatcgatac
    55921 ttccagtcat tatattcaat aaaggccctc gtgtagtaaa gtatataaat attttctatg
    55981 caatgatagc taatgttcca atcattattg tgcaacgcta taatcgaccg agattaacgc
    56041 agttactacg catattaaaa cgaagaggtg aacgtcatga ctaaacatat catcgttatt
    56101 ggtggtggct taggtgggat ttctgcagca attcgaatgg cacaaagtgg ctattcggtc
    56161 tcattatatg aacaaaataa tcatatagga ggcaaagtga atcgtcatga gtcagatgac
    56221 tttggctttg atttaggtcc atctatttta acgatgcctt atatttttga aaaattattc
    56281 gaatatagca agaagcaaat gtcagactac gttacaatca agcgattgcc acatcaatgg
    56341 cgtagctttt ttccagatgg aacgactatc gatttgtatg aaggtattaa agaaacaggt
    56401 cagcataatg cgatattgtc gaaacaggat atagaggaac tgcaaaatta tttgaattat
    56461 acaagacgaa tcgatcgtat tactgaaaaa gggtatttta actatggttt agatacacta
    56521 tctcaaatta ttaaatttca tgggccatta aatgcactta ttaattatga ttatgtacat
    56581 actatgcaac aggccataga caagcgtatc tcgaatccat acttgcgaca aatgttaggc
    56641 tattttatca aatatgtagg ttcttcatcg tacgatgcgc cagctgtatt atctatgtta
    56701 ttccatatgc aacaagagca aggcctttgg tatgtagaag gtggaatcca tcatttagcc
    56761 aatgccttgg aaaagctagc gcgtgaagaa ggtgtcacaa ttcatacagg tacacgtgtg
    56821 gacaatatta aaacatatga aagatgtgtg acgggtgtca gattagatac aggtgagttt
    56881 gtaaaggcag attatattat ttcaaatatg gaagtcatac ctacttataa atatttactt
    56941 caccttgata ctcaacgatt aaacaaatta gagcgggaat ttgagccagc aagctcagga
    57001 tatgtgatgc atttaggtgt tgcttgccaa tacccgcaat tagcacatca taatttcttt
    57061 tttacggaaa atgcttatct caattatcaa caagtttttc atgaaaaggt attgccagat
    57121 gatccgacca tttatttagt aaatacgaat aaaactgatc acacacaagc gccagtaggt
    57181 tatgaaaata tcaaagtctt accacatatt ccatatattc aagaccagcc ttttacaact
    57241 gaagattatg cgaagtttag ggataaaatt ttggataaat tagaaaaaat gggactcact
    57301 gatttaagaa aacacattat ttatgaagat gtttggacac cggaggatat tgaaaaaaat
    57361 tatcgttcta atcgtggtgc aatatatggt gttgtagcag ataaaaagaa aaacaaagga
    57421 tttaaatttc ctaaagaaag tcagtatttt gaaaacttgt actttgtagg gggatcagta
    57481 aatcctggtg gtggcatgcc aatggttaca ttaagtgggc aacaagtcgc agacaaaata
    57541 aacgcgcgag aagcgaagaa taggaagtga tatctatgaa atggttatca cgaatattaa
    57601 cagtaatagt gaccatgtct atggcgtgtg gtgcattgat atttaatcgt agacatcagc
    57661 taaaggcgaa aacgctgaac ttcaatcata aagcattaac aattattatt ccggctagaa
    57721 acgaagaaaa aagaataggt catttactac attcgataat acaacagcaa gttccagtag
    57781 atgtcattgt tatgaatgac ggatcgacag atgaaacagc atgtgtagcg cgttcatatg
    57841 gtgcgactgt agtagatgtt gttgatgatg ctgacggcaa atggtatggg aaatcacatg
    57901 cttgttatca aggtgtgacg catgcatgta cgaatcgtat tgcttttgta gatgctgatg
    57961 taactttctt aaggaaagat gctgttgaag cgttgattaa tcagtatcaa ttacaaggtg
    58021 aaaaaggatt gttaagcgta cagccttatc atataacaaa gcgtttctac gaagggtttt
    58081 cagcaatatt taatttaatg acagtcgttg gtatgaatgt attttctacc ttagacgacg
    58141 gtcggactaa ccagcatgca tttggaccgg tgacattaac aaataaagaa gattattatg
    58201 caactggagg tcataaaagt gcgaaccgtc atattattga aggatttgct ttaggaagtg
    58261 catatacttc acaatcattg cccgtaacag tttatgaagg atttccattt gttgcatttc
    58321 gcatgtatca agaaggattt cagtcattac aagaaggatg gacaaagcat ttgtcgactg
    58381 gggcaggtgg cacaaagcct aagatcatgg cagcaattgt gttgtggttg ttcggttcta
    58441 ttgcgagtat tttagggcta tgtcttagtt taaaatatcg ccaaatgtct gtaggaaaaa
    58501 tgttaacagt ttacttgagc tatactacac aatttattta tctgcatcga agggtcggcc
    58561 aattttctaa tttattaatg gtatgtcatc cattgttatt tatgtttttt actaaaattt
    58621 tcatccaatc ttggaaacaa acgcatcgtt atggtgtagt tgaatggaaa ggtcgtcaat
    58681 attctatatc taaagaacaa taaatcaagg taatggcatt tcaatatagg aggactagta
    58741 tgacaatgat ggatatgaat tttaaatatt gtcataaaat catgaagaaa cattcaaaaa
    58801 gcttttctta cgcttttgac ttgttaccag aagatcaaag aaaagcggtt tgggcaattt
    58861 atgctgtgtg tcgtaaaatt gatgacagta tagatgttta tggcgatatt caatttttaa
    58921 atcaaataaa agaagatata caatctattg aaaaataccc atatgaatat catcactttc
    58981 aaagtgatcg tagaatcatg atggcccttc agcatgttgc acaacataaa aatatcgcct
    59041 ttcaatcttt ctataatctc attgatactg tatataaaga tcaacatttt acaatgtttg
    59101 aaacggacgc tgaattattc ggatattgtt atggtgttgc tggtacagta ggtgaagtat
    59161 tgacgccgat tttaagtgat catgaaacgc atcagactta cgatgtcgca agaaggcttg
    59221 gtgaatcgtt gcaattgatt aatatattaa gagatgtcgg tgaagatttt gaaaatgaac
    59281 ggatatattt tagtaagcaa cgattaaagc aatatgaagt tgatattgct gaagtgtacc
    59341 aaaatggtgt taataatcat tatattgact tatgggaata ttatgcagct atcgcagaaa
    59401 aagattttcg agatgttatg gatcaaatca aagtatttag tattgaagca caaccaatca
    59461 tagaattagc agcacgtata tatattgaaa tactggacga agtgagacag gctaactata
    59521 cattacatga acgtgttttt gtggagaaga ggaaaaaagc aaagttgttt catgaaataa
    59581 atagtaaata tcatagaata taggtggttg aataatgaag attgcagtga ttggtgcagg
    59641 tgtcacagga ttagcagcgg cagcccgtat tgcttctcaa ggtcatgaag tgacgatatt
    59701 tgaaaaaaat aataatgtag gcgggcgtat gaatcaatta aagaaagatg gctttacatt
    59761 tgatatgggc cccacaattg tcatgatgcc agatgtttat aaagatgtat ttacaatgtg
    59821 tggtaaaaat tatgaagatt atattgaatt gagacaatta cgttatattt acgatgtgta
    59881 ttttgaccgc gatgattgta taacggtgcc tacagattta gctgaattac agcacatgct
    59941 agaaagtata gaacctggtt caacgcatgg ttttatgtcc tttttaacgg atgtttataa
    60001 aaaatatgaa attgcacgtc gctatttctt agaaagaacg tatcgcaaac caagtgactt
    60061 ttataatatg acgtcacttg tgcaaggtgc taagttaaaa acgttaaatc atgcagatca
    60121 gctaattgaa cattatattg ataacgaaaa gatacaaaag cttttagcgt ttcaaacgtt
    60181 atacatagga attgatccaa aacgaggccc gtcactatat tcaattattc ctatgattga
    60241 aatgatgttt ggtgtgcatt ttattaaagg cggtatgtat ggcatggctc aagggctagc
    60301 gcaattaaat aaagatttag gcgttaatat tgaacttaat gctgaaattg agcaaattat
    60361 tattgatcct aaattcaaac gagccgatgc gataaaagtg aatggtgaca taagaaaatt
    60421 tgataaaatt ttatgtacgg ctgatttccc tagtgttgcg gaatcattaa tgccagattt
    60481 tgcacctatt aaaaagtatc caccacataa aattgcagac ttagattact cttgttcagc
    60541 atttttaatg tatatcggta tagatattga tgtgacagat caagtgagac ttcataatgt
    60601 tatttttgca gatgatttta gaggaaacat tgaagaaata tttgaagggc gattatcgca
    60661 tgatccttct atttatgtgt atgttcctgc ggttgctgac aaatctcttg caccacaagg
    60721 acaaacaggt atttatgttt taatgccaac accagaactt aaaacaggta gcggaatcga
    60781 ttggtcagat gaagcattaa cagaccaaat aaaagatgtt atttatcgta aattagcaac
    60841 tattgaagta tttgaagata taaaatcgca tattgtttca gaaacaatct ttacgccaaa
    60901 tgattttgag caaacgtatc atgcgaaatt tggttcggca ttcggtttaa tgccaacttt
    60961 agcgcaaagt aattattatc gtccacaaaa tgtatcgcga gattataaag atttatattt
    61021 tgcaggtgca agtacgcatc caggtgcagg cgttcctatt gtcttaacga gtgcgaaaat
    61081 aactgtagat gaaatgatta aagatattga gcaaggcgtg taagggagta gtctaagaga
    61141 aagatgtgag aaaagtataa ggggaacgta aaaggcatag tgaaataagt atgtgcttaa
    61201 gaagttttat ttaacaatga atgacgcctg ttaaggttca cgtttgaata cgtaccttga
    61261 tgggcgttat actttatata agcatatcta actggttgcg catgcggata tgcatggcag
    61321 ttaaatccta acgattgagc gtatattaat ctgaaaattc ctaatattga caataatatt
    61381 gtacctacct aaaatgaaaa taaattcatt caatacaacg tgtgtcataa gatctgttga
    61441 agataacggt gaatggcgat gtcattgtaa ccatctaaat gagagtgata caagttgtta
    61501 ggattatgat agtcgcccag gctatgttta atgatagagg aggaatttga tgatctctaa
    61561 caaattagca aatatccctg atagttattt tgggaaaacg atgggacgta aaatcgaaca
    61621 tggaccattg ccattaatta atatggcagt tggaatccct gatgggccca cgccacaagg
    61681 tattattgat cattttcaaa aagcactaac tattcctgaa aatcaaaaat atggtgcttt
    61741 tcatggtaaa gaagcgttca agcaagctat tgtagatttt tatcaaagac aatataatgt
    61801 gacgttagat aaagaagatg aagtatgtat tttatatggt acaaaaaatg gattagtggc
    61861 agtaccaaca tgtgttatca atccaggaga ctatgtatta ctacctgacc caggctacac
    61921 tgattattta gcaggtgtac ttttagctga tggcaagcct gttccgctta atttagaacc
    61981 gccgcattat ttgccagatt ggtccaaagt tgattcaaaa ataatagata aaacaaaact
    62041 catttattta acgtatccaa ataatccaac tggatcgaca gctacgaaag aagtttttga
    62101 tgaagcgata gctaaattta aagatacaga cactaaaatc gtgcatgatt ttgcttatgg
    62161 tgccttcggt ttcgacgcta aaaatcctag tatacttgct tcggaaaatg ggaaagatgt
    62221 tgcgattgaa atttattcct tgtctaaagg ttataacatg tcaggtttta gagtcggttt
    62281 tgcagttggt aataaagata tgattcaagc tttgaaaaag taccaaacgc acacaaatgc
    62341 tgggatgttt ggtgcattac aagatgccgc aatatatgct ttgaatcatt atgatgattt
    62401 tttagaagaa caaagcaatg tatttaaagc acgtcgtgac cgattcgaag ccatgcttac
    62461 aaaagctgat ttgccatttt ttcatgctaa aggtggcata tatgtatggt tggaaacacc
    62521 gcctggttat gacagcgagc aatttgagca atttttagtg aaagagaagt caatacttgt
    62581 tgctccaggg aaaccatttg gagagaatgg taatcgttat gtgagaatct cattggcgtt
    62641 agatgaccag caattagacg aagctgcaat aagattaaca gaactagcat atttatatga
    62701 ataataaagt taagggtgat gttaaaatga caaaaattaa aatcatgagt gtacgtgatg
    62761 aagatatgcc ttatatcaag gcgtgggcag agaaacatca tgttgaggta gatattacta
    62821 aagaagcatt aactgatgac aatgttgaag gtgtcgctgg ttatgatggc ttatcgctat
    62881 cacaacaaat accattatca gagcatgttt ataaacgatt aaatgaacta ggtattaaac
    62941 aaatcgcaca acgtagtgct ggatttgaca catatgattt ggagttagca aacaagtata
    63001 acttgattgt gtcaaatgtg ccgtcatact ctccgaattc aattgcggag tttgctgtga
    63061 accaggctat taatgtggtt cgtcatttta atcaaattca aacgaaagtg cgagagcatg
    63121 attttagatg ggaacctact attttatcga aatccattaa agatttaaaa gtagctgtta
    63181 ttggtacagg tcgaattggc cgtgtagtag ccgatatatt tgccaatggt tatcaaagtg
    63241 atgtggtcgc atacgacccg tttcctaatg ctaaaattgc aacgtatgtc gattacaaag
    63301 atacgattga ggaagcggtt gaaggtgctg atatcgtgac attacatgta cctgcaacga
    63361 aatataatca ttatctattt aatgctgaat tatttaaaca ttttaaaaag gacgcagtat
    63421 ttgtcaattg tgcgagaggt tctttagtag atactaaggc tttattagac gcattagaca
    63481 atggtgtgat taaaggtgca gcacttgata cgtatgaatt tgaacgcaaa cttttcccaa
    63541 gtgatcaaag gggcaaaaca ctgaacgatc cattgttaga atcgttgatt gacagggaag
    63601 atgtcatatt aacaccacat attgcgtttt atactgaagc tgcagttgaa aatctaattg
    63661 ttgatgcatt agatgcaaca ttagatgtat tgcagactgg agatactagg ttacgagtaa
    63721 attaaaaatc gaactgatga gataatttgg attgttggga ttctcatcag ttcgattttt
    63781 taatttggtg ttgatgacgt tgaatattgc ctaattaaac gacatcgtaa ccttgatctt
    63841 caattgcgtc tttcatttga gatacagcaa ctttactgtc atcatattga acacttactt
    63901 gaccattttc aaggttaact tcagctgaag tgacaccgtc aatattattt aatgcagatt
    63961 caacagcact tttgcagtga ccacagctca taccttcaac atttaaaatt tcttgtgaca
    64021 tagcattcac ctccttttag agc
//
LOCUS       DAMZPP010000002        45120 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMZPP010000002 DAMZPP010000000
VERSION     DAMZPP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 45120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45120
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.002"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(21..248)
                     /locus_tag="POH73_000068"
     CDS             complement(21..248)
                     /locus_tag="POH73_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886058.1"
                     /translation="MLIEERGLKMTELNEIINAIQSLFESQSGYKISKNSGVPYQTVQ
                     DLRNGKTKIEDARFRTIIKLYSYYTSLKEQS"
     gene            complement(205..351)
                     /locus_tag="POH73_000069"
     CDS             complement(205..351)
                     /locus_tag="POH73_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886059.1"
                     /translation="MKLFYIAFLIIIWLNIFLGNEVIHTLTVLITTLYIVNRRKRVKN
                     DRVE"
     gene            1019..1978
                     /gene="hyl"
                     /locus_tag="POH73_000070"
                     /pseudo
     CDS             1019..1978
                     /gene="hyl"
                     /locus_tag="POH73_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
     gene            2432..2665
                     /locus_tag="POH73_000071"
     CDS             2432..2665
                     /locus_tag="POH73_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886060.1"
                     /translation="MVFKDWEINVVYSGKHEVVTNENSLFVIDEDYDVAIAINYLDNK
                     LKVSHVNYGSEFTIDASNKVLALMIHNPNIDEN"
     gene            3275..3460
                     /locus_tag="POH73_000072"
     CDS             3275..3460
                     /locus_tag="POH73_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886061.1"
                     /translation="MNKHINKKTTKHLEDYKKVQQRNSEETNRGCLKSAAMLIVLFLF
                     IIFGIVACSTHVNFFFQ"
     gene            complement(3709..4059)
                     /gene="scc"
                     /locus_tag="POH73_000073"
     CDS             complement(3709..4059)
                     /gene="scc"
                     /locus_tag="POH73_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-C"
                     /protein_id="HDI6886062.1"
                     /translation="MKFKKYIVAGTLAVLLSTTAVSTLDGNKADASSKKDYIIQSEFH
                     DKRIAEELKSLLDQSYVNDLAAGSLNPYYKRMIMMNQYRAKAALKSNNFAKMAEAKVG
                     LENIYKEIDEIINR"
     gene            complement(4211..4708)
                     /gene="efb"
                     /locus_tag="POH73_000074"
     CDS             complement(4211..4708)
                     /gene="efb"
                     /locus_tag="POH73_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HDI6886063.1"
                     /translation="MKNKLIAKSLLAIAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNTTPKYIKFRHDYNIVEYNDGTFEYGARPQFNKPAAKTE
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYNVKKMILQERIDQVLK
                     QGLVR"
     gene            complement(4965..5471)
                     /locus_tag="POH73_000075"
     CDS             complement(4965..5471)
                     /locus_tag="POH73_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886064.1"
                     /translation="MIIKILTILLLLCILSYLVTNKKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
     gene            6118..6528
                     /locus_tag="POH73_000076"
     CDS             6118..6528
                     /locus_tag="POH73_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HDI6886065.1"
                     /translation="MYFTGRTYYEKNITKVIIASTVIATGLLTHSNDAKAFFSYEWKG
                     LEIAKKLADQAKKDDERATKLIDEGFKGETVNDLYIIVKKLSKGDVKKAVVKMRNGGP
                     KDFYTFDLTRPLEENRKNIPLVKNGEIDSIYWDQ"
     gene            6494..6577
                     /locus_tag="POH73_000077"
     CDS             6494..6577
                     /locus_tag="POH73_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886066.1"
                     /translation="MVKSTRSIGINKHTELNSCTPNVGKQC"
     gene            complement(6876..7193)
                     /locus_tag="POH73_000078"
     CDS             complement(6876..7193)
                     /locus_tag="POH73_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding protein"
                     /protein_id="HDI6886067.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKAEKYNEYQTNFK
                     KQVNKKVVDAQKAVNLFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLVLKYN
                     TLK"
     gene            complement(7387..7554)
                     /locus_tag="POH73_000079"
     CDS             complement(7387..7554)
                     /locus_tag="POH73_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886068.1"
                     /translation="MKRIVLFGKVIVDCPIWLNPVDYLVKSVLLKSIKLRFQEVRKFE
                     ILVKIMNRIHI"
     gene            complement(7684..8187)
                     /locus_tag="POH73_000080"
     CDS             complement(7684..8187)
                     /locus_tag="POH73_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDI6886069.1"
                     /translation="MSKWIVVSDNHTESGILYQIYEAHPDADMYLHLGDSEFEYDDTE
                     LSLFRRVKGNCDFYPEFENEAVAKCNDVRAFYTHGHLYQVNRTRYLLAEQARELGCVF
                     AFYGHTHVAKYEYINGVHAINPGSISQSRSSMEESYAEVLIDDQTLQGTVNFKNRQHE
                     TISHTTF"
     gene            complement(8180..8767)
                     /locus_tag="POH73_000081"
     CDS             complement(8180..8767)
                     /locus_tag="POH73_000081"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDI6886070.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELILDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DRTMAQLSKEQKGQISHRRNAINLLEAYLAGDQNV"
     gene            complement(8779..9579)
                     /gene="racE"
                     /locus_tag="POH73_000082"
     CDS             complement(8779..9579)
                     /gene="racE"
                     /locus_tag="POH73_000082"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDI6886071.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDTTHITNIIKEWLNLSVNVERISVND"
     gene            complement(9818..10633)
                     /gene="sdhB"
                     /locus_tag="POH73_000083"
     CDS             complement(9818..10633)
                     /gene="sdhB"
                     /locus_tag="POH73_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HDI6886072.1"
                     /translation="MTEQSVKNTPQHEIQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(10633..12399)
                     /gene="sdhA"
                     /locus_tag="POH73_000084"
     CDS             complement(10633..12399)
                     /gene="sdhA"
                     /locus_tag="POH73_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDI6886073.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(12451..13065)
                     /locus_tag="POH73_000085"
     CDS             complement(12451..13065)
                     /locus_tag="POH73_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HDI6886074.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(13389..15170)
                     /gene="uvrC"
                     /locus_tag="POH73_000086"
     CDS             complement(13389..15170)
                     /gene="uvrC"
                     /locus_tag="POH73_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDI6886075.1"
                     /translation="MEDYKKRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGSKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            15336..15431
                     /locus_tag="POH73_000087"
     CDS             15336..15431
                     /locus_tag="POH73_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886076.1"
                     /translation="MFDYHLTFIDLLIPPFSSRKQYDDLSFNFIY"
     gene            complement(15494..15808)
                     /gene="trxA"
                     /locus_tag="POH73_000088"
     CDS             complement(15494..15808)
                     /gene="trxA"
                     /locus_tag="POH73_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDI6886077.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(15981..18329)
                     /locus_tag="POH73_000089"
     CDS             complement(15981..18329)
                     /locus_tag="POH73_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDI6886078.1"
                     /translation="MRQKTLDVLEFDKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARCSRSIKGTKPIFKEERTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKNADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEDAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(18339..20051)
                     /gene="polX"
                     /locus_tag="POH73_000090"
     CDS             complement(18339..20051)
                     /gene="polX"
                     /locus_tag="POH73_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HDI6886079.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPRAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITLDDING
                     DIHMHTTFSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNI
                     KLKK"
     gene            complement(20124..20645)
                     /locus_tag="POH73_000091"
     CDS             complement(20124..20645)
                     /locus_tag="POH73_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDI6886080.1"
                     /translation="MIIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMIALFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(20646..20912)
                     /gene="zapA"
                     /locus_tag="POH73_000092"
     CDS             complement(20646..20912)
                     /gene="zapA"
                     /locus_tag="POH73_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDI6886081.1"
                     /translation="MAQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
     gene            21288..22226
                     /locus_tag="POH73_000093"
     CDS             21288..22226
                     /locus_tag="POH73_000093"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDI6886082.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNTANSSLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAVLHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFITYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKNYGISRLDSISKKHFKNREKAQKILKPL"
     gene            complement(22528..24930)
                     /locus_tag="POH73_000094"
     CDS             complement(22528..24930)
                     /locus_tag="POH73_000094"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDI6886083.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIFVFSESQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELELSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQVRLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     HTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVQVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFEKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFAMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVIGFIGELHPTLAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(24930..25988)
                     /gene="pheS"
                     /locus_tag="POH73_000095"
     CDS             complement(24930..25988)
                     /gene="pheS"
                     /locus_tag="POH73_000095"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDI6886084.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEEKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(26369..27109)
                     /locus_tag="POH73_000096"
     CDS             complement(26369..27109)
                     /locus_tag="POH73_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDI6886085.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPQYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
     gene            complement(27349..27456)
                     /locus_tag="POH73_000097"
     CDS             complement(27349..27456)
                     /locus_tag="POH73_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886086.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKRSLI"
     gene            complement(27493..27816)
                     /gene="isdG"
                     /locus_tag="POH73_000098"
     CDS             complement(27493..27816)
                     /gene="isdG"
                     /locus_tag="POH73_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HDI6886087.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(27835..28569)
                     /gene="srtB"
                     /locus_tag="POH73_000099"
     CDS             complement(27835..28569)
                     /gene="srtB"
                     /locus_tag="POH73_000099"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDI6886088.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQAHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKILNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDKIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(28631..29596)
                     /gene="isdF"
                     /locus_tag="POH73_000100"
     CDS             complement(28631..29596)
                     /gene="isdF"
                     /locus_tag="POH73_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HDI6886089.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(29609..30487)
                     /gene="isdE"
                     /locus_tag="POH73_000101"
     CDS             complement(29609..30487)
                     /gene="isdE"
                     /locus_tag="POH73_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HDI6886090.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(30484..31560)
                     /gene="isdD"
                     /locus_tag="POH73_000102"
     CDS             complement(30484..31560)
                     /gene="isdD"
                     /locus_tag="POH73_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HDI6886091.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTSSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KDLDNKEMLYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDIKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTVKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     VGIVLATILLISAHLYSRKRGNQV"
     gene            complement(31560..32243)
                     /gene="isdC"
                     /locus_tag="POH73_000103"
     CDS             complement(31560..32243)
                     /gene="isdC"
                     /locus_tag="POH73_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HDI6886092.1"
                     /translation="MKNILKVFNTMILALIIIIATFSNTANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKERIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPSDVAGANAPGKDDKN
                     SASGSDKGSDGATTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            32452..33516
                     /gene="isdA"
                     /locus_tag="POH73_000104"
     CDS             32452..33516
                     /gene="isdA"
                     /locus_tag="POH73_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HDI6886093.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQAVLNNASFWKEYKFYNANNQELATTVVNDDKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKAEK
                     VKPAVTAPSKNENRQTTKVVSSEATKDQSQTQSARTVKTTQTAQDQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKQNEVHKQGPSKDSKAKELPKTGLTSVDNFISTVAF
                     ATLALLGSLSLLLFKRKESK"
     gene            33718..35676
                     /gene="isdB"
                     /locus_tag="POH73_000105"
     CDS             33718..35676
                     /gene="isdB"
                     /locus_tag="POH73_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HDI6886094.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAKAAEET
                     GVTNTEAQPKTEAVASPTTTTTEKAPEAKPVAKPVANAVSVSNKEVVAPTTETKEAKE
                     VKAVKEVKAPKEAKEEKPAAKADNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDF
                     EMKKKDGTQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKL
                     VSYDTVKDYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKT
                     EEDYKAEKLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEETKKALDEQVKS
                     AITEFQNVQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVME
                     TTNDDYWKDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQ
                     YHVRIVDKEAFTKANADKTNKKEQQDNSAKKETTPAMPSKPTTPPVEKESQKQDSQKD
                     DNKQSPSVEKENDASSESGKDKMPVTKPAKAEVESSSTTPTKVVSTTQNVAKPTTASS
                     ETTKDVVQTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEES
                     NKDMTLPLMALIALSSIVAFVLPRKRKN"
     gene            complement(35830..36003)
                     /gene="rpmF"
                     /locus_tag="POH73_000106"
     CDS             complement(35830..36003)
                     /gene="rpmF"
                     /locus_tag="POH73_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDI6886095.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(36083..36640)
                     /locus_tag="POH73_000107"
     CDS             complement(36083..36640)
                     /locus_tag="POH73_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDI6886096.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            36767..37906
                     /locus_tag="POH73_000108"
     CDS             36767..37906
                     /locus_tag="POH73_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDI6886097.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNNIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKHAKNINAISIKRENAQHHDSLIQHHKFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTESLKNIYTVTEGFENRLKSYINEATDFHHFV
                     KSLKTKRYTYTHIQRLLMNVLLNIKPTDVTRNIHAVKVLAMNDRGRQYLKHLKTVFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            complement(37968..38450)
                     /gene="coaD"
                     /locus_tag="POH73_000109"
     CDS             complement(37968..38450)
                     /gene="coaD"
                     /locus_tag="POH73_000109"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDI6886098.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(38452..38994)
                     /gene="rsmD"
                     /locus_tag="POH73_000110"
     CDS             complement(38452..38994)
                     /gene="rsmD"
                     /locus_tag="POH73_000110"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDI6886099.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGIDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            39064..39453
                     /locus_tag="POH73_000111"
     CDS             39064..39453
                     /locus_tag="POH73_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886100.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            complement(39456..39710)
                     /locus_tag="POH73_000112"
     CDS             complement(39456..39710)
                     /locus_tag="POH73_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDI6886101.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            39949..40878
                     /locus_tag="POH73_000113"
     CDS             39949..40878
                     /locus_tag="POH73_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI6886102.1"
                     /translation="MKKVNRNILNAVLVGAGFVSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQRSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGINLTSSRFIKWLNDMNVIPGYYGVNSI
                     DLMNDLYQKGAHTIVTDRPDLAQQFKQSINNKQ"
     gene            complement(40945..41379)
                     /locus_tag="POH73_000114"
     CDS             complement(40945..41379)
                     /locus_tag="POH73_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDI6886103.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(41395..42432)
                     /locus_tag="POH73_000115"
     CDS             complement(41395..42432)
                     /locus_tag="POH73_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDI6886104.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGENVNVSPLKIGQHSAEIFNHTSINPEPSFTVDGKKYEFELS
                     DEDLKTQTLIKYGNIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYRLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            complement(42759..43220)
                     /locus_tag="POH73_000116"
     CDS             complement(42759..43220)
                     /locus_tag="POH73_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HDI6886105.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREIDKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            complement(43245..44156)
                     /locus_tag="POH73_000117"
     CDS             complement(43245..44156)
                     /locus_tag="POH73_000117"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDI6886106.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLLGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            44607..>45120
                     /locus_tag="POH73_000118"
     CDS             44607..>45120
                     /locus_tag="POH73_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HDI6886107.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAII"
BASE COUNT        15052 a         8036 c         6167 g        15865 t
ORIGIN      
        1 ctctttattt aatgatttaa ttatgactgc tcttttaatg atgtataata actatatagt
       61 tttataattg ttctaaatct agcatcttct attttcgttt taccattcct caaatcttgt
      121 acggtctgat acggcacccc tgaatttttc gatattttgt agcctgattg agactcaaac
      181 aaagattgaa tagcgttgat aatttcattc aactctgtca tttttaaccc tctttcttct
      241 attaacaata tacaatgttg ttattaaaac agtcagtgta tgaatgactt catttcctaa
      301 aaatatattc agccatataa taattagaaa tgcgatataa aatagtttca ttataatccc
      361 ctatcatttt tatatttatc actttccact tagtttcaac attaaaagct aacctaagtt
      421 taagattctc ttataacgcc tataactaga agtctacatt ttcccttgta taacatattt
      481 tacaatgttt tataatatta cactacaccc ttttacattt ttatacgaaa ttacaatact
      541 ttgttaaaat taattcgcac aaatattcca agacaattaa tttttctcta ttcataattg
      601 attaattata ctagttaata tacagttaac ttttatttaa tagttaatta attgatttaa
      661 ttctaagata tttgtatttt tatattgtaa tcgattacat tttaaagtta aataaatacc
      721 cagttttaaa gaaattaatc aataaaatta gctttgtctt ttccttggtt cataaagagt
      781 tcattcaact ttgactaagc ctcgacattg aaatgcttcc tttcaaatat taattaaaag
      841 ttaaaaacat attcttaaaa tattcactca tcatcactca atcatttaac agttgctaca
      901 ttaaatatac taatataaac gatgataaat atttgatatg tctcaactgc attattctaa
      961 attgacatat tgattattgt ttcctcaaat cgttttaaaa aatagaagga tgatgaaaat
     1021 gaaaacacgt atagtcagct cagtaacaac aacactattg ctaggttcta tattaatgaa
     1081 tcctgtcgct aatgccgcag attctgatat taatattaaa accggtacta cagatatagg
     1141 aagcaatact acagtaaaaa caggtgattt agtcacttat gataaagaaa atggcatgca
     1201 caaaaaagta ttttatagtt ttatcgatga taaaaatcac aataaaaaaa tattagtcat
     1261 cagaacgaaa ggtaccattg ctggtcagta tagagtttat agcgaagaag gcgctaataa
     1321 aagcggttta gcctggcctt cagcatttaa ggtatagttg caactacctg ataatgaagt
     1381 agcacaaatt tctgattact accctagaaa ttcaattgat actaaagaat atatgagtac
     1441 attaacttat ggattcaacg gtaacgtgac tggtgatgat tctggaaaaa ttggcggttt
     1501 gattggtgca aatgtttcaa ttggtcatac actcaaatat gttcaaccag atttcaaaac
     1561 aattttagaa agtccaactg ataaaaaagt aggttggaaa gttatattta acaatatggt
     1621 gaatcaaaat tggggaccat atgacagaga ttcttggaac ccagtatatg gcaatcaact
     1681 tttcatgaaa acaagaaacg gttcaatgaa agcagcagag aacttccttg accctaacaa
     1741 agcaagttct ttattatctt caggattctc accagacttt gctacagtta ttactatgga
     1801 taggaaagca accaaacaac aaacaaatat agatgtaata tacgaacgag ttcgtgatga
     1861 ctatcaacta cattggactt caacaaattg gaaaggtacc aatactaaag ataaatggac
     1921 agatcgttct tcagaaagat ataaaatcga ttgggaaaaa gaagaaatga caaattaatg
     1981 gttatgtaca ctcaaatagt cacataataa tattagccga taacatcaga aatgatgtgc
     2041 gtcggctatt ttagttgcag tataaaatta aacatcatga atagtaatat acctattatg
     2101 cttttcagta tatattcacg gctaaaatca tgattacggt actgatttct taactacaaa
     2161 catttgaaat tcattgttgt caaacccttt aattaaaagt tttgaaattg ttcaaaactt
     2221 attgaacaca ttatgtgtta atatatttgc agaattattt taaaatcgtt ttaaaactat
     2281 actgaaataa gaagataaaa agtataataa gtacatataa ttgaaaataa tttctagaaa
     2341 tctatttatc tctaatctag atattgtaag aagttgtatg aataagttaa acttagattg
     2401 aatcagttta aatgttcaag gaggatgaca aatggttttt aaagattggg aaattaatgt
     2461 tgtttactca gggaaacatg aagtagttac aaacgaaaat agtttatttg ttattgatga
     2521 agattatgat gtcgcaatcg caatcaatta tttagataat aaattaaaag ttagtcatgt
     2581 taactatggc tctgaattta caattgacgc atctaacaaa gtacttgcgt tgatgattca
     2641 taatccaaat attgacgaga attaaatatt aaaaaacatt tagaaacata aaggcatact
     2701 atatatagtc ttttaaacgt ataacgaacg aaaattagaa attcggatat tcattacact
     2761 attaaaatgc aatttttcaa agcatttaaa aacaacagtt aaggtggtga ccaaaaatta
     2821 acattgtgta taacatcatt gtatatatag catgcatgat taacttcgga ctgattatcg
     2881 tcccattgct aactaccaat gacgcagatt ttgtttggac ttcttcgtca ttaatatata
     2941 tgatcctttt aacactactt atattaattg tttatattaa aactaaagat agtttacagc
     3001 taacaatatt tatacttagt gtggcattaa tccttctata ctgtgcacct ttactgttca
     3061 tttatgtatt ctaatgtttc attaatattt taatacgtca gtttaaaaac aaacaaattg
     3121 gaaaataaac gaaacaagtt atcatctaat ttaacatgat actcacttaa aatttgaaac
     3181 aacgagcagc ctaacttaag ctttatatct ttatttcaag ttatgttttc aactatttta
     3241 atgcaattta catttagatt aaggagtaaa catcatgaat aaacatataa ataaaaagac
     3301 cacaaagcat cttgaagatt ataaaaaggt tcaacaaaga aattcggagg aaacaaatag
     3361 aggttgttta aaaagcgccg ccatgcttat tgtcttattt ctattcatta tatttggcat
     3421 cgtggcatgt tcgacacacg ttaacttttt cttccaataa tatactcatt gagtaaaaat
     3481 cagaacgttt catactacgt ttaaatttat ctatgggagc actataaata ttttttagat
     3541 tgtacaaagc aattttgtgc ataatatttc taactattat atttatattt gtctccccta
     3601 ttttttgtat acattgctca tacataaatt ttaaattgca gaatttttaa ctgtacaaat
     3661 aaaaaaacag gcgaagacaa ttctattttt gtctctgcct gttttttatt atctatttat
     3721 aatttcatca atttctttgt aaatgttttc taatccaact ttagcttcag ccatttttgc
     3781 gaaattatta ctttttagtg ctgcttttgc tctatattgg ttcatcataa tcatacgttt
     3841 gtagtatggg tttaagcttc ctgcagctaa atcatttaca taagattgat caagtaatga
     3901 tttcaattct tcagcaattc gtttatcatg aaactcactt tgaattatat agtctttctt
     3961 actacttgca tctgctttat tcccatctaa cgttgatact gctgttgttg ataatagtac
     4021 tgctaatgtt cctgctacta tatatttttt aaatttcatt gtaaattctc cttttaaaat
     4081 tgatacttaa tgattatata gtcaatttta ggaaagttac attaacctgg cattaaataa
     4141 catagtcgtt ttattaataa aaaataatat aatattaaat tattttgaac ttataataat
     4201 gacagaagta ttatctcact aatccttgct ttaatacttg atcaattcgc tcttgcaata
     4261 tcattttctt aacattgtat tcaaatgata ctaagttgac tgccttttgt gcttttctat
     4321 gtgcgctcac agtatgtgtt ttttcaaatt ctctcacaag attttgggct tgaatcaatt
     4381 tttgttcttt tttaatagtt gcctcagttt tcgctgctgg tttattaaat tgtggacgtg
     4441 caccatattc gaatgtaccg tcattatatt ccacgatgtt ataatcatgt ctaaacttaa
     4501 tatatttagg tgttgtatta aattttggtc tagattgata tttaaaagta ccatcattgt
     4561 actctacgat attgtgatta atactcactg gtttcttttc tcttggaccg tatccttcgc
     4621 ttgcatctgc agttgacgca attgtagttg tagtaatacc aattgccgct atcgctagta
     4681 atgattttgc tatcaattta tttttcatgt taattatcct ccaaattatt atcttacatt
     4741 tattaagtta tcctatttat acaacttttg aaattaacta gttattaatt aactataaac
     4801 actttataat atagacaaaa tatagttatc tatcttgtgt ctcatcaata cttatacact
     4861 agtcaaaaat ttcatgcctc tacttttcga atttcaaaaa taaaactaca caacaaaaag
     4921 acaccatttg aatgttggtg tctgatggta taatttcata gattttattt gtaacttctc
     4981 attctatagt taaatattgt aacccagcta ataatcaaag ttggtagaat tactaaattc
     5041 aaattagaca cgtcaaaaaa gactgattgg aaattgtcga tattaatatc attaatgtcc
     5101 attttttcat ttactgcttg atagatcgca aaataaacta agaaccattg aattaaatgt
     5161 tctaaaacga caacctctgt taaatcgaca ttcttttttc taaaaagtaa tctaactata
     5221 aataattgga atatacttga aatcaagatc aaaataatac ccattaagaa aaaactacca
     5281 aaatcaattg tgataaattg aataatacca taaataatta cggcagatat tgccagtgaa
     5341 atatatccta taaagataaa caccacaccc ataaagagtg tctttaagaa cagaaaaggc
     5401 ttctttttat ttgtaaccaa atagctcaat atacaaagta gtaatagaat tgttaaaatt
     5461 ttaattatca tcgtttgacc tcaatatgtt tatttatatc tttattattg accacgaaaa
     5521 aataacaatc acttaagtga ttgccaataa tactcaatgc ctactctata gtaatcttgg
     5581 cacgtgattt tacactattt tatctattta aaaatgtaag tgttgaatta tgcttacttc
     5641 gaaaatgcat aatacaacta aatagtatat tttttaactt atatattaat cataacatat
     5701 caaccttaat tagaatatat ctcttatttc tttataagta aaattataca tattaattac
     5761 ttctctacta tgtgtcattt ctatgtttta taaacattaa ttatcaaaaa cgctatatgt
     5821 ataacaattt ggactacttg tctacccaac attgcaatta aatgtagaga aatataaata
     5881 tcgttttaac aataaaaatc attcaatcat agttgagtga cgtttactaa tattattata
     5941 ctatcaataa attcaataat acctcaaata aaaataataa catcaaattc atcaatgttc
     6001 tttttcaatt gcattaaacc ttattaattt gcacttaaat ttaattaatg aagtgttaac
     6061 tggtaaatgc ttgaaaccgt ttgtaaaata gcagtgtact cgcattagta agtacaaatc
     6121 tattttacag ggagaactta ttatgaaaaa aatattacaa aagtaattat tgcatctaca
     6181 gtcatcgcta caggtcttct aactcattca aatgatgcta aagctttctt tagttatgaa
     6241 tggaaaggat tagaaatcgc taaaaagtta gcagatcaag ctaaaaaaga tgatgaacgt
     6301 gctactaaat taattgacga aggttttaaa ggtgaaactg ttaatgacct atacatcatt
     6361 gtcaaaaaat taagcaaagg cgatgtgaaa aaagctgtcg ttaaaatgag aaatggagga
     6421 cctaaagatt tctatacttt tgacttaact cgtcctttag aagaaaacag gaaaaatatt
     6481 cccttagtta aaaatggtga aatcgactcg atctattggg atcaataaac acactgaatt
     6541 aaatagttgc acgccaaacg ttggaaaaca atgctaacgt tttggcgtgc ttttttatat
     6601 gctgcaactc ccaatttatt tatcatcttt acgactgttt aataaagcac gtatgattaa
     6661 cacaatacct ctaaatccgc gaaaattcta acgattgtta atacatatgc ttggcgttat
     6721 cccgtcattc aaaacaccta tcacatacat tttcaaagga ttttctaaga tttaccccaa
     6781 tatactcatc aagtaaaata catacaatgt ttaaataact ttcatcagat tacacatagt
     6841 aaaaaagcca cactctatag catggcttct atcaattatt ttaaagtatt atattttaaa
     6901 actagatcga tttgtctttg taattttttc ttttcatagc tgtgttggaa atgaattaag
     6961 ttaacagctc tttgtgcttt acggtgtgtt gcaacagttc ttgtacgttt gaacaagttt
     7021 acagcttttt gtgcatccac aactttttta tttacttgtt ttttaaagtt tgtttgatac
     7081 tcgttatatt tttcagcctt agtttgtgca tgagattcac cggcaaatgt tgataccgtt
     7141 aaactaatag cagctatgct taaaattgat ttcccaataa aattcttttt cataattaat
     7201 aacacctatc ctcaaatatt tattttgtat atatttataa taatagacag aagcaaaaaa
     7261 ctacattaac tatttattaa ctaaacatta atatttgtgt cactacattt ctgaatttca
     7321 agaattttgc tactccatat tttaaccatc aaattattga tataatttat gcctttagaa
     7381 atccttttaa atatgtatac gattcatgat tttaactaaa atttcaaatt tcctgacttc
     7441 ttggaatctt aattttatac tttttaataa tacactttta accaaataat caacaggatt
     7501 caaccaaata ggacaatcaa caatcacttt cccaaaaagt acaattcttt tcataacata
     7561 aatgtgttat tcataccagt taccttctaa aaattgtcag caacactttt aaacaaacat
     7621 tcatgtcacg acctcctagt taaatcagtc tataaactaa aaaaagccat agctactttt
     7681 aatttaaaaa gtagtatgac taattgtttc atgttgtcgg tttttaaaat tcactgtacc
     7741 ttgtaatgtt tgatcatcaa tcaaaacttc cgcatacgat tcttccattg aacttctaga
     7801 ttgagatata cttccaggat taatagcatg aacaccatta atatactcat attttgcaac
     7861 atgtgtatgt ccataaaaag caaatacaca acctaattca cgtgcttgct cagctaataa
     7921 atatcttgtt cgattgactt gatataaatg tccatgagtg taaaatgctc tcacgtcatt
     7981 acattttgcg accgcttcat tttcaaattc tgggtaaaaa tcacaattgc cttttaccct
     8041 acgaaataag ctaagttctg tatcatcata ctcaaattct gaatcgccta aatgcaaata
     8101 catatcagca tctgggtgtg cctcgtaaat ttggtataaa atgccagatt cagtatgatt
     8161 atcactcaca acaatccatt tagacatttt gatcacccgc aagataagct tctaataaat
     8221 taattgcatt tcgtctatgg ctaatttgac ctttttgttc tttagaaagt tgcgccatgg
     8281 ttctatctaa tttcggtaca taaaatatcg gatcatatcc gaaaccattt tcgccatatt
     8341 tcccatctgc aatttcacct gaaacagtac ctttaaatac ttttgtttcc atatcagggc
     8401 cactcatact tatgacacaa acaaattgtg cacgacgatc agttgtatta ccaagcttat
     8461 ttaataattt ttcaatattt gcttcatcgc ttttattttc accagcataa cgtgcagagt
     8521 atatacctgg ctcaccattt aatgcaaaaa cttctagtcc actgtcatca gctatgaccg
     8581 ttttattcaa tgcttttgca gcagcttctg attttaatat agcattttct tcaaatgttg
     8641 atcctgtttc ttccacatca aaatctagta ttagttctga aatacctatt acgtggtaat
     8701 ctggaaatat cactttaaag tcatttattt tcccttgatt attcgatgca ataacaatct
     8761 ctttcattaa aaatcctcct agtcattcac tgatatacgt tccacattga cagataaatt
     8821 tagccattct ttgataatgt tagtaatatg tgtggtatca cctgttgcaa aaaatcgatg
     8881 atctggatgt tcagtataac ttgcatgttc attactaaat gttagcaatg cactaacttc
     8941 acgagccgtt tctaatccag acgaaatcac tgtcttttta ccaccaaaat aatcatagat
     9001 aggtttatag agcaatggat agtgggtaca tcctaaaatg acagtatcag actcactatt
     9061 acgccaacgt ttcagtgttt gatgaataac aatgcttgta attgttggat cactatatct
     9121 catttgttct acaagtggca caaaacctgg acaggcaacg ccatgtacct ctacatgtgg
     9181 atttatacgt ttaatatgtg ttcgatatgc ttcagattta attgtgcctt ccgttcctag
     9241 tactaataca ttttgatttc tagtcgtcat tattgctgtt ctagcacctg gttcaattac
     9301 gccaatcact gggattgata aggtcttttg taaatattct aaagctacag cagttgcagt
     9361 attacaagca atcacgagca tttttatatc aaattccatt aatttacgag cgatttcaac
     9421 tgtatattgt tttacttgtt ctcctggtct tggcccatat ggacatcgcc caatatcacc
     9481 taagtaataa atcgtctcat ttggcaactg acgcataatt tctttagcta ctgtcaaacc
     9541 tccgacacca gagtctatta cacctattgg tttattcata ttcggtcatc cttactttta
     9601 atttaatttt tagtttatca taactaagca ttggatttta gtattatgca ctgtgtttac
     9661 catttttgtc attataatat aatacttatt ttaaattagc tcactatcat attgtcatgt
     9721 aatgtaacta taaaaaatcc cttctaagtt tttgtgtatc tccattcaat ttaattttga
     9781 aaggaacata acattttaac tcaaaaggga ttaattttta ttctacttca tggtctgaac
     9841 caaagaatga tttaaacatg tggaatgttg tttctctgtt cattgctgca atggatgttg
     9901 ttaatggaat acctttaggg caagcattaa cacagttttg tgaattacca cattgctgta
     9961 agccacctgt acccattaat gcatttaaac gttcatcttt agtcatagat cctgttgggt
    10021 gcaaattaaa caaacgaact tgcgagattg cttgcgcacc aacgaattta ttattttcag
    10081 taacattagg acaaacttct aaacatacac cacatgtcat acatttagat aattcataag
    10141 ctgtttgacg ttttttctct ggcatacgtg gccccggacc taaatcatac gttccatcaa
    10201 ttgggatcca tgctttcata cgttttaagt tatcgaacat tctagaacga tcaacttgta
    10261 agtcacggat aactgggaaa gtattcattg gctctaaacg aataggttgt tctaattgat
    10321 caacaatcgc agaacaagat tgtcttgcac gaccattgat aaccatagaa catgctccac
    10381 atacttcttc taagcagttc atatcccaga caacaggtgt tgttttttca cctttaatat
    10441 taactgggtt acgtctaatt tccattaaac atgcaatgac gtttaaattt tcacgatatg
    10501 gaatttcaaa tgtttcttca taaggcttag aatcacttgt atcttgtcgt ttaataatta
    10561 attttactgt tttttgtttc ggtttagatt gtatttcatg ttgtggagtg tttttcactg
    10621 attgttcagt cattattttt tacccccttt agacttactt gtgtaatcac gtttacgagg
    10681 tggtattaaa ctcacatcga cgtcatcata agtaaactgt ggtttttcaa atgcgccttg
    10741 gaatgaggcc attgtcgttt ttaaccactc ttcatcatta cgctctggga attctggttt
    10801 ataatgggca ccgcgtgatt cgttacggtt atatgcacca atcgtaatca cacgtgcaag
    10861 tactaacatg ttccataatt gacgagtaaa gaataccgct tggttactcc aagtttgagt
    10921 atcttccata tcaatatctt cataacgttt catcaattca acaatctttt tatctgtttc
    10981 taacagtttt tcattttcac gaacaacagt tacatttgct gtcataattt caccaagttc
    11041 acggtgtaat ttatatgcat tttctgtacc gcgcatagct aataatttat caaaacgttc
    11101 ttgctcttca gctttacgtt tttcaaaaat actttcgtcc atatcagtat atgatcgatc
    11161 aatatttgaa atataatcaa tcgcgtttgg acctgctact gtaccaccat aaatcgctga
    11221 taacaatgaa ttggcaccta agcggttacc accatgttga gagaagtcac attctccagc
    11281 tgcaaataac cctttaatat ttgtcatttg atcataatct acatatagac cacccattga
    11341 atagtggaca gctgggaaaa tcttcattgg tactttgcgt gggtcatcac cagtgaattt
    11401 ttcataaatt tcaatgatac cacctagttt tacatctaac tcatgtggat ctttatgtga
    11461 caaatcaaga tataccatgt tttcgccatt aatacctaat ttttggttaa tacatacatc
    11521 gaaaatttca cgcgttgcga tatcacgagg tactaagtta ccataatcag gatatttctc
    11581 ttctaagaag taccaaggct taccatcttt atatgtccaa attcgtccac cttcaccacg
    11641 tgctgattca ctcattagtc gtagtttatc atcaccaggt attgcagtag gatgaatttg
    11701 aatgaactca ccattagcat aaatagcgcc ttgttggtaa acaatggaag ccgctgatcc
    11761 tgtattaatc attgagtttg ttgttttacc gaaaataata ccagggccac ccgttgccat
    11821 aataactgca tctgaaccaa atgtttcaat ctcagcagtt gtcatatttt gtgcaacgat
    11881 acctcttgca ctatcatcgt cacctttaac tatgccaagg aattcccatc cttcatactt
    11941 cgtaactaat ccatctactt catatgcacg aacttgttca tccaatgcat ataataattg
    12001 ttgtccagtt gttgcccctg catatgctgt tctgtggtgt aatgtaccac cgaaacgtct
    12061 aaaatctaat agaccttcat ttgttctatt gaacattacg cccatacggt ctaataaatg
    12121 aataatttta ggtgctgcct ctgtcatcgc tttaacaggt ggttggtttg caaggaagtc
    12181 gccaccatac actgtatcat caaagtgaat ccaaggagaa tcgccttccc ctttagtatt
    12241 gaccgcacca ttaatgccac cttgggcaca aacagagtgc gaacgcttta ctggtacaac
    12301 tgagaacaaa tctacatgtg cacctttttc tgccgcttta attgttgaca ttaagcccgc
    12361 taggccacct ccgacaacaa taagatgttt ctctgccata aaaatttcac tcccctaaat
    12421 tttcaatcta tatttgttaa atgcgatgta ttacataaag gcaataattg cagtaacacc
    12481 aatatacgaa ataactaaga atacgattaa tgaaacccat gtaaatactc gttgtgattt
    12541 tggagattga agtccacccc aagtaactaa gaatgaccat aagccatttg caaagtggaa
    12601 cacaacagca ataatacaaa taatataaaa tattgcccat ccaggatgtt gcaatgtttc
    12661 atgcattaaa tcgtaattca cttctttgcc gtaaaatgct ttttgtaaac gtgtttgcca
    12721 taaatggata ccaataaaga taaatgttaa gataccactc actctttgga agaagaacat
    12781 ccagtttcta aaaatcgagt aatgtccaac attttctttt gctgtaaatg caatgtgtat
    12841 accaaacaaa ccgtgatata acaacggaat gtatataaat aaaaattcta caataattag
    12901 aaatggtaat gattccataa agttagatgc tttattaaac gcttcagcac cttgtgttgc
    12961 ttggtgattc actaataaat gaacgaccaa aaatgcacct attgggataa tacctaataa
    13021 cgagtgaata cgtcttagat aaaattcatt ttttgattga gccaaaagga gtcccccctg
    13081 tgaacgaata tttaatttat tgagctattt atattaaacg tacgcttaac cccctaaagt
    13141 gatatcgttt ctagcgttgt cattatcatt aagcgaaaca ttttaaagac aaatacacac
    13201 tgcacgatca ccaaactgca tgtcgaacaa tgtaaaattt ggatttaata tttaagattg
    13261 cttgtgatga taaactttct catttagaaa acgcttccac gtacattcaa aaaaataact
    13321 ttgttaacca tattgtaaca ttatttcata tattttgggg catgagaatg attctcacgc
    13381 cctgtaattt atttatgcaa ttgttcatgt aggttctttg caacgttttc aggaatacct
    13441 atatttttaa aatcttcaag tgtagcttcc ttcattttct tgattgaacc gaatgaacgc
    13501 aataataatg ttttacgttt gctaccgata ccatctatat catcaaggat tgatttcaag
    13561 cctgtctttt gacgtgtttg tctatgaaat gtgattgcga atctgtgaac ctcatcttgg
    13621 atacggtgca acaaataaaa tgcttggcta tttttcttca gtggtacaat ttctgcacta
    13681 gcgccatata ataattcaga tgtttggtgt ttatcatttt tctgcaaacc tgcaacaggg
    13741 atatcaagac ctaattcgtt ttgtagcaca tcaataaccc cgttcatatg tcctttacca
    13801 ccatctacta ttattaaatc aggtaatggt aatccttcgt ttaaaacgcg agaatatcgt
    13861 cgtcttacta cttctctcat tgatttgtaa tcatctggac ctttaaccgt tttgatttta
    13921 tactttctat aatttttctt gtctggttta ccgtcgacaa atgtaaccat tgctgacact
    13981 ggatccacac cttgaatatt agaattatcg aatgcttcaa ttctaattgg tgtttgaatt
    14041 cccatttgtg ttccaagttc ttcaatagct ttaattgttc tggactcatc acgtgatatt
    14101 aattcaaatt tattatttaa ggatacttta gcgttatgtg cagctaagtc aaccatatct
    14161 tttttgggac ctcgcgcggg ttgaacgatt ttagtgtcca caacagattg aatcatttct
    14221 ttatccaaat tacgtggtac atgaacttcc ttaggtaaaa tatgttggtt taagctataa
    14281 aattgtccaa taaatgtata aaattcttct tcttctgttt gctgtaatgg aatcatcgtt
    14341 gtatctcgct ttatcatatt accttgtctt ataaagaaaa cttggataca catccatcct
    14401 ttatcaacac tataaccaaa gacatcacga atcgttttat ctgatgacat aattttttgt
    14461 ttgttagtta gattttgaat atgttgaatt aaatctctgt attctttagc tcgttcaaaa
    14521 tcaagtgatt cacttgcagt taacattctc tcttctaaac tttttaaaat tgttttgtct
    14581 tccccattca gaaaatcagt aatttccttc gtcatttgtg cgtatttact caaatcaacg
    14641 tcatatacac atggtcctaa acattgtcca atatggtaat aaagacataa tttatctggc
    14701 atcttatcac atttgcgata tggatatatt ctgtctaata actttttagt ttcttgagca
    14761 gaatatgcat tcggatacgg tccgaaatat ttaccagtac cttgttttac agttctcgtc
    14821 actagtagtc taggatattt ttccttcgta attttaataa atggataact tttatcatcc
    14881 tttaataata tattatatct tggttgatat tgtttaatca gatttaattc aagtaaaagt
    14941 gactctgttt cactagacgt gacaataaac tcaaagcgac gtatttcacc aaccagtctc
    15001 gttgttttag catcatgagc gcccgtaaaa tatgatcgca atcgatttct tagcttttta
    15061 gctttgccaa catatatcac ttgatcgtta cgatctttca ttaaatagca gcctggttcc
    15121 ataggtacga catttaattt atttttaatt cgtttcttat agtcttccaa aaatatcccc
    15181 ccttctaatt ttaaaatgaa tgatgctacg aaagcgcttc aaatttatca ccgctaattc
    15241 tagttcataa tcaacatctt cgagtacgac ttttcatgca catcatgtaa tattgaacat
    15301 tgttttatca aaccaactat tacatatcta tcagcatgtt tgattatcat ttaacgttca
    15361 tagatttatt aatcccacca ttctcaagtc gtaaacaata tgatgactta tcattcaact
    15421 ttatatatta aaaacacaaa caaaaactgg agcaattcat taaagaaatg ccccagtcgt
    15481 cattggttgt aacttataaa tgtttatcta aaacttcagc taagttttct tttggttgga
    15541 aaccaacaac tttatcaact ggttgaccgt ctttaaagac gattaatgtt ggaatactca
    15601 tcacttcata tttagctgca gttgatggat tttcatcaac atctaatttt aaaatgtcag
    15661 ctttaccttc atagtcagct gctaattctt ctaataccgg agcgatcatt ttacatggac
    15721 cacaccatgt tgcccaaaaa tctactaatt gtacaccaga ttctactttt gaatcaaaat
    15781 ctgcatctgt tactttaacg attgccataa atgccaatcc tccttaatta ttattatgat
    15841 gttcaaatta tatcagataa catgcttcat gtatttctat ttgctcaatt tttatgctta
    15901 tttcaaataa catgtttgaa atgtattctt gttgctttac attattttaa ctgcagctaa
    15961 ttaatttatc aaattataat ttattttagt gttgcaacgg taacgccaaa tccaccttcg
    16021 cttggcatac cacctctaaa gtcactaaca cttttatgct ttttcaaatg ttgttgtaca
    16081 cctttttgaa gtgcacctgt acctttacca tgaatgatat aaacttgttc gtaattactt
    16141 aaaacggctt gatctaaata ttgatctagt tcaattaaag catcctcata acgatagcct
    16201 cgtaagtcaa gttcagtttt aattgtttga cgattttgac gtgtaaccat tttcgttggt
    16261 ttaacttttt ctttttgttt tttctctaaa tcttcaatag gtaacttcat tttaataatt
    16321 cccatttgaa caattgcttc ttcatcattg acaatttcta aaacttcacc cttttgaccg
    16381 taagataata cttttacttc atcaccagca acaattttat cgtatttttg cttttgtaca
    16441 ttttgcttta tagatttcgc ttcataatga tcatctaatc gtttcttctt atcaatcaat
    16501 tcatgttctt taacatctgc acctttttgt tcacgcaatt gtcttaggtc tttaataata
    16561 tcgtcagctt cttttgttgc tgctttaatt ttttgattcg ctttttcttt agcatcttca
    16621 attagagatt tttcataatt ttggaattgt tgatactgct tagataaatc atcatgcact
    16681 tgctccgctt cttttacaag acggtccagt tctaacctct gtgtctctac acgtttgtaa
    16741 ttacgctcta atgattcaat catttcattt atttcttttt catcagtacc aatcatcgtc
    16801 ttagccttat taataatatt caaactaaga cctaactttt tagaaatatc aaaagcattt
    16861 gaacgacccg gcacacccat taataactta tacgttggac tcaatgtatc tacatcaaat
    16921 tctacactcg cattcataac gccttctcga ttataactat atgctttaag ttcaggatag
    16981 tgcgtcgttg ccattactag agaaccaatt tttctaacat gatctaaaat gctcattgct
    17041 aatgcagcac cttcacttgg atctgtacct gcacctaatt catcaaataa aactaaacta
    17101 tgtttgtctg cattctttaa aatttcaact atattcgtca tatgagatga aaaagttgat
    17161 aatgattgtt ctattgattg ttcatctccg atatcgcaat atacgttttt aaatacactc
    17221 aactgactac catcaagtgt tggaatcaac aatcctgatt gagccataac aataattaaa
    17281 cctaatgttt ttaatgttac tgttttacca cctgtattcg gtcctgtaat aattaccgtt
    17341 tcaatatctt ccataaattc gatggtatta gctacaacag tctcacgatt taataatgga
    17401 tggtatgctt taggtaaata tacagtacgt tcctctttaa atatcggctt tgttccttta
    17461 atacttctac tacatctcgc ttttgcgatt aaaaaatcta actgacccat gacttgttct
    17521 gccacaagta gtgcatcttt gtccgcagca acataaccag ttagttgcgt taaaatgcgt
    17581 tctttttcaa ttgcttcatc atgacgtaat cgactaattt gattattcat ttcaacaact
    17641 gatgatggct caatatacaa tgtttgtcct gaagcagatt gatcatgtac aatcccatta
    17701 aaatcttgtc gatattcagc tttgacaggt ataacgtttc tttcattcct aactgttaca
    17761 atagcatctg ataatttttt ctgatttgct tggcttttaa caatacggtc caaattttgt
    17821 ctaatacgtt gattcgtgct agaaatttta cttctaatcc cttgcaattc ataactcgca
    17881 ttatcatata aatcatacgt atcgcatgtt tcatttattt gttgaaaaag atcagttaac
    17941 acaggtaatt gattcatctt gtcatctaat attgggtatt taacaccttc atcttcttca
    18001 accaattgat tataaaatgt cttaaattga ttttgtactt gaattaatct ttttatcaag
    18061 ttaagctctg atacatttaa aacgccgcca atatcagcgc gatgaatgaa tgctgatact
    18121 ttagataagc cactcaagct tggtaaacga tgcttattat agatttgagc aatctcatcc
    18181 gtttcttcca tttgaaaaac aaccgtttca aaattagtag ctggcatcat ttgattgacc
    18241 ttttccaagc ctaagtcact aattgtttca ttggcaacga gtgattttat tttatcaaat
    18301 tctaagacgt ctaatgtttt ttgtctcata aaatccctct atttcttaag ttttatatta
    18361 ttttcaataa agtctttaaa agcttcacgc gataatgcgt taatcactct atcttttgtt
    18421 acaaatcctt tttgcgcagt tgctacgcca taattcataa aatctaaatg atttgtatga
    18481 tgcgcatcag tgttaatagt taatttcaca tttggatatt tacgaacgat atcagcgctc
    18541 aaatccagtc gatgtggatt ggcattaatt tctaatactg tattcgtttc ttcagctaat
    18601 gccattaatt gttcaatatt cggtttataa ccatctcttc tacctataat acgccctgtt
    18661 ggatgcgcta tatgtcgcac gtatggattg cgacatgcat tagctaatcg ttccataatt
    18721 tgttcttctg attggttaaa gctttgatga atagctccaa ttacgtaatc aagttgtgct
    18781 aaaatttcat catcataatc cagcgagcca tcaggtaata tatccatttc tgtacccgaa
    18841 taaatatcaa tttcactata ttctttatct aaagctttaa tttcttcgtt ttgtcttaaa
    18901 agtctttcta cttgtaagcc attagcaaca cgtaaacttt gtgaatgatc agtaattacc
    18961 atgaatttat aaccttttgc gatatttgct tctaccatgt ctcgaatgga aaacgcacca
    19021 tcactaaacg ttgtatgcat atgaatatca ccattaatat catctaatgt aatgatatta
    19081 cttagatctt tatcaaattc gctaccatct tctctcatag caggtggtat aaaattcaca
    19141 ttaaaatgtt catatatttt ggcttcacta tcatattgaa ttaatgtacc atcagcttgt
    19201 tcaattccat attcacttac tttttcatcc cgtgctttag caagttgtcg aattcttata
    19261 ttatgttctt ttgacccagt aaaatgctgc aatgtatgat aaaaagccct tggttcaatt
    19321 aatcgaaaat cgacaccaat cgtttcatca tcatacgcta attctaatga aacttttgtg
    19381 ttccccactg caacttcttt tactttattg ggaatattta ataattgctg ctgcactgct
    19441 tttgggttat cggtacttat tatgaaatct aaatctttgc tcatttcttt aaaacgacgg
    19501 aagcttcctg cagatgaata ttgatcgata taatttaatg tatctatata atcaatgatt
    19561 tcttgattaa gtcttctcat ttgatcaatt ggatatctat ctttcttagc accaagttgt
    19621 ttcacagctt ctaatatgtt ttgttccgtt ttcttagcaa atccgcttaa ttcactaact
    19681 tttccatttt cacaagcaac ttgaagtgaa gctttatcaa caatattcaa ctctttatat
    19741 agcttagcaa ttttcttgct tccaagtcct tgaattttca aaagtggaat aagaccttcc
    19801 ggaacttctt cctgtaattg ctgtaaatac tgagattcac cggtctcacg gtaatcattg
    19861 attacttctg caacaccttt accaatgcct tttaactccg ttacatcaga tatttcatct
    19921 aatggtcgtt catctaattc aagactttga gctgcttttc gatacgctga tattttaaaa
    19981 gtattttccc cttttaattc catataagta gcaatttgtt ctaatagttt gataacatct
    20041 ttttttgtca taataacact ccataaaaag aagaccagga cgtatcatta atatatacct
    20101 ttgtcctgac ctcttatgat aatttataaa ttaatggtga aagccgataa atatgggatg
    20161 tgaaaaatca atgaatgact cacaatagat attttaagct gatgttgtaa tgcttcgtta
    20221 ggatataatg ccaatagata taacgtaaag tgtaagacaa ttatcgtcat aaacacacca
    20281 actatcattc ccattgcacg actgaaaata tgaatgtttt gatacgctat tattttatca
    20341 aaagttacga taattagata taaaatgaac ttacaaaaca atgcaatcat taaaaaagct
    20401 acaatcgctt caaatcgatt ttgtagatga ttaaaatgaa acgcaaaagt tgtattaaat
    20461 gctgttgttt taggatatgg aataaataca attaatcttt ctacaataga tttgtaaaat
    20521 tgactggcaa tccacaatga tacaatcgtt gcactcaaat gtatcataga taaccaaaaa
    20581 cctcgtctga atccaacgat gacaaaatac acaaagaaaa tgattatgat aaaatcaatg
    20641 atcatttatt gctcacgctg ctgcaatttg tgaatttgtt gtttcaaacg tcgattttct
    20701 tcttctagta gtactttttc atgcataata ttcacagcag ttagtattgc ttttcttgaa
    20761 gtatctaaac ctgctgcttt ataccctaat tcttttattt tatcatcaac taaatgtgct
    20821 acatatcgta tgtgctctgg gttatcttcc ccaacaattg taaaaagctg atcattaatt
    20881 gatacattta ccttgttttt aaactgtgcc atttataatt tctcctgatc ctttttttaa
    20941 aatctaaatt cacgttataa aatatgactg gatagtttgt ctgaatttga tactaatatt
    21001 gttatattgc aattatgata aaacaacaac acaatctcta tagatgactt aatgttcttt
    21061 ttataatgaa atgaaataat gtaaagaatt ttctattcaa tactttacca tgtttaaatt
    21121 gtgtcactat aacattttca taaacattat acatgaccac tatgtatttt gtaagtatcc
    21181 gcaattaatt ctttacaaca tatataaatg tttctgacgt tattatcatt tatgatataa
    21241 ttatttttgc taatgataat gaaattatta cgaaaggatt tacacaaatg gcgaatatcg
    21301 tttttaaatt gtcggataaa gacataacga cattaatgtc acgcatttct tttgatactg
    21361 agaatttacc tcaaggtatg aaagcacgtg caaagtatca aaatacaact gtaaatattt
    21421 accaatcagg taaagtcatg tttcaaggta atcatgcaga agctgtgtct gaagaacttc
    21481 taccgcagca ttctcaattg aatactaata aaactaaaaa gaaaaatacg gccaattctt
    21541 ctcttgaaca gactttaatg tatgatcaat ttaactgtat tggtagtgat gaggctggaa
    21601 gtggcgatta ctttggtccg ttgactgtgt gtgctgcttt tgttaccaaa gagcatgtcc
    21661 ctattttaaa aactttgggt gtagatgatt caaaaaaatt aaccgatact aaaattgtag
    21721 aacttgctga gcaattggtt actttcatcc cacattcact attaacacta cacaatgaaa
    21781 aatataatat tcagcaagca aaaggctgga ctcaagtaaa aatgaaagct gttcttcata
    21841 atgaagccat caaaaatgta cttgaaaaaa ttgattcgtc tcaattagat tatatcgtca
    21901 ttgatcagtt cgcaaaacgc gaagtgtata gccattacgc attatctgat ataccacttc
    21961 caaagaaaac aaaatttgaa acaaaaggag aatcgaagtc attagccatt gcagtggcaa
    22021 gcataatttc tagatacgca tttattacat atatggatca aatatcaaaa aatattaata
    22081 tgacgattcc taaaggtgct ggtgctaaag tcgatgtaat cgccgccaaa ataataaaaa
    22141 attacggtat ttcacgttta gattccattt caaaaaaaca tttcaaaaat cgtgaaaaag
    22201 cccaaaaaat tttaaagcct ctttgattta taatcattag aggcttttat gcagttgtgc
    22261 aaagcatcaa ctgtaatccg ctgagcctct atgatttata atcaagataa attggatcct
    22321 aataggaaat cctattaggt aagtcatact aatttgcttt gttcataagt atgactttat
    22381 atgcagttgt gcaaagcatc aactgaaaac cactcttcct ctttgattta taaatgaaat
    22441 ggaacctaat aggtgaatcc aaaaataaaa ccccatgtta gtgatatcaa tctacttcag
    22501 atatccttat acatggggtt taaatcatta tctaataaca gcaccttgtt caattaatgc
    22561 tgcttcaatt tccgcttgta cttttgaaac gcgttcatct gtcaatgttt cttctgtgtc
    22621 taaataattt aaacgtattg caattgattt tttacctttt tctaaatgtt cgccctgata
    22681 tacatcaaag acaagtgtat cttttaatat attgccaccg tgtgcatgaa tcgttgataa
    22741 taaatcagct gctggaatat tttgatctac ttctaatgca atgtcacgag acatgcctgg
    22801 gaatctcgga attggctggt aattaatgta acctaccgac acagccatta atgcatcaaa
    22861 attcaactca aaaacatacg tacgttttaa atcattatca gctgctaatg ttgggtgtaa
    22921 ttcaccaata aaaccaataa ctttattctc taataagatt tcagctgtac gacctggatg
    22981 taatccatca atatcagcac gacgataact aaattcaagg tttaactttt cagatactcg
    23041 atccacgaca ccttttgcta aataaaaatc aaccgtttct ttcttacctt gccattgatt
    23101 gactacataa tctccagtta aaataccact taaatattca acttgatctg gtagttcacc
    23161 ttcaccatta gcaaagaaga cattgccgat ttcaaataat tttacatctt tatttttacg
    23221 tgccacatta tatgatgccg cttcgattaa atgtggtaat aaactttgac gtaatgacgc
    23281 atgcgcttca ctcattggca tcaataaatc aattgtttga cgctgttgca tcgcaaatgc
    23341 agtagcatct tctttagaaa ctaacgaata cgtaatagct tggtctaatc cagcaccttc
    23401 taacacttct ttaaccattc tcgttttata ttggcgatca gttaactgac cactagtaac
    23461 tttttcgaag acaggtaatg ttgaaggaat atcatcgtaa ccataaatac gtgcaacttc
    23521 ttcaattaaa tcttctttaa ttgtaatatc tttgcgacgt gacggcactt gcactgtaat
    23581 aacatcatca tttatttctg tatcaaaccc taattgatta aaaatagtaa caatatcatt
    23641 ttgtgacaaa tcaaatccaa tagtgcgatt aattttatca gcagtgatgt cgattggtgt
    23701 aataaatgca ccaagttctc ctgaagacac tctatctttt agcacttttc cgtttgcata
    23761 agtttgtaat aaataacatg cacggtcgac tgcttcatct acaaattcag tagctattcc
    23821 tttttcaaaa cgactagatg attcactgcg taaatttaaa cgtcttgatg tatgacgaat
    23881 tgaaactgga tcaaaaatag caccttcaat aactatattt gatgtatgtt ctttaacttc
    23941 tgaaaaatcg ccacccataa caccagctaa tgcaattgga gtttgtccat tagtaataac
    24001 aatatcgctc gttaataatt cacgttctgt atcatctaat gttgtcattt tttcgccttc
    24061 attagcttga cgaacaacaa tctgttgtga accaatcgca tcttgatcaa acatgtgcaa
    24121 tggttgaccg tattctaata acacataatt tgagatgtcg acaacattat taataggacg
    24181 tatacctgct tttattaagc gtacttgcat ccaaattggc gaaggttcaa ttgtcacgtc
    24241 gtgaacaaca cgtgcactat aatatggtac tttatcttca ttttcaattg tcacagtcag
    24301 ttcatcattt gcagataact caagctcatt tgatgttgtc tctggcttag tcatttttgt
    24361 attatataat gctgcaactt cataagcagt acctatcata cttaaagcat cagcacgatt
    24421 cggcgttaaa tcaaattcca ttacttgatc atctaaatat aaagcttgta aggcatctgt
    24481 tcctggaact tgggattcac taaatacaaa aatgcctgat tcaaaacttt tcggtatata
    24541 gttacttgaa ataccaattt cttgtaacga acaaatcata ccttccgaac gttcaccgcg
    24601 taatttggca cgcttaattt taataccacc aggcaatcta ccacctactt tagcaacaat
    24661 gacatattgt cctgcatcaa cgttcggggc accacatacg atttgtacag gttcgtcttc
    24721 tccgatatca acttggcaaa catttaattt atcagcatca ggatgtttct cttttgactt
    24781 aacgaagcca acaactaaat ttttgatatc ttttgtgtag tcaattaaat catccacttc
    24841 aatacctgtg cgcgtaatac gttctgccaa attacttaca gaatcatcga ttgttacata
    24901 ttctttcaac cattcatttg atatcaacat tacatgtcac ctctatcttc taccgcttta
    24961 aattgatcta aaaatctcac atcattagta tagaaatgac gaatatcttc tataccatat
    25021 ttcaacattg caatacggtc tggtcccata ccaaatgcaa atccagagta ctctgaagaa
    25081 tcaaaaccag ccatttctaa tacattagga tgtaccattc cagcgcctaa aatttcaatc
    25141 catcctgtgt gtttacacac attacaacct tttcctttac atttaaaaca tgacacatca
    25201 acttctacag aaggttcagt gaatgggaag taacttggac gtaaacgaat ttcacgatca
    25261 gcaccaaata atttcttagc taacaattct aaagtacctt tcaaatcact cattttaacg
    25321 tttttatcaa caactaatcc ttcaatttgt gtaaattgat gactatgtgt cgcatcatca
    25381 gagtcacgac gatacacttt accagggcaa ataattttaa ctggaccttg accatgacgt
    25441 gattccatcg tacgtgcttg cactggtgat gtatgcgtac gtaataaaat ttcatccgta
    25501 atatagaaac tatcttgcat atcacgtgca gggtgtgatt taggtaaatt cagcatttcg
    25561 aagttataat gatcttgttc aacttcatat ccattgacaa tttcataacc taaacctaag
    25621 aataagtctt caatttcttc tattgtacgt gttaatggat gctttgaacc gatttcaata
    25681 tgacgacctg gtaatgatac atcaattgtt tcttcagcca attgcttatt taatttttct
    25741 ttaactaaca tctgttgtct ttcatctaat tcattttgaa ttgtttgacg caattcattc
    25801 actttttgac caaacgcagg cttctcttca ttcggcaaat ccttcatcaa tttcattagt
    25861 ccgctaactg accctttttt acctaagtat ttcactttaa cttcttgcag tgcacgttca
    25921 tcatttgctt cattaatatc tacaagcgct tgttgtttta actctgacat tgtttgttgt
    25981 tcagacatat gtcactgacc tccctattcg aactattcat tttctattta tcatatttgt
    26041 tgtttaacta aaaaaagact ttcatcctct taaaaaataa gggacgaaag tcatttccgt
    26101 ggtaccaccc aaatttatgc ctaagcatac acttgtttaa tattgataac ggattgtctt
    26161 tccgacttaa atctctttaa gtaaccaaaa aggtgaaaag actttaaata attggaataa
    26221 cttgcagtca cgattattct ccctaaaaca attatttata taaatgtcca tgccttttta
    26281 ttggtttata ttcaattgtg tatgcaatca cgtagaaatg ttctgttaaa caattaatta
    26341 taattatatc ggaaaacttt caacacggtc aacctttcaa atgataaagt aaaatactac
    26401 ctgcaatcgc tacatttaaa ctttcagctt taccataaat aggtatgatt aaattttgtg
    26461 tagtatgtgc taataattca ggattaacac cttctccctc attacctaat agtaatgcaa
    26521 aagaatcact tgaagtaact tctttatatg ccactgcgtt ttcaagtgct gtaccataaa
    26581 caggaccatt aaactgagta ataaacgtat cgagatcttg tgtcataact ggcaaatgaa
    26641 aaacactacc ttgactcgct cgcaacactt tatcttgata aggatctgtc gtaccctttt
    26701 ccattattac agcatccatt ccagcagcat ccgcagttct aattaatgtg cctaaatttc
    26761 caggatcttg aacacgatcg ataagcaata cttgttgtgc tttagaaata tcatattgtg
    26821 gcttctcaat gattgcgaaa aacccttgtg gtgtcactgt acctgataaa gattcagcaa
    26881 ctttcatgtt tatttcaaaa acttcttgcg cgtatgcgat aatttgctga tctaatcttg
    26941 ccggttcaat tgcaaataat tgtgtaatta caatcccact ttgataagct tcttcaatta
    27001 aatgcacacc ttcaattaaa gctaatccag ttttatccct ctcacgtttc ttttttagct
    27061 tgttcgcttg tttaattcta ttattttgtg cagaagtaat ttgttccata gatagctcct
    27121 cgctttattt ttaaaaataa aaatattaat cattaataag atgaaaacat ttgattgtat
    27181 agttaatatt aattaatcga ttttatcact catattattt caaattgtat aaatttcttt
    27241 tatcgatact actactataa atcatacgcc ccaaaatatc attattaatt cttttcttct
    27301 tcaaaataaa tcaaaatcat atatttgatg attattttca aagcacattc atatcaaact
    27361 acgttttagc aatttgttgt tagcatgttt gtgttcatta aaaaaacgac catcatcggt
    27421 atcatgtatg gtcgttacaa aagctaacaa taccaattgt cataacaagt actgcaacct
    27481 ttttaaattc aattatttca tgtaactata gcctatatca tatgtaatta ctttgttatt
    27541 aataatcggg ctactttcat cttcattttt acttctaaca tgtttatgtg ctgctttaaa
    27601 gacatcagat tttaaccaat ccgtaaaagc ttgctttgat ttccaaactg ttaaaatttt
    27661 cacttcatca aaatcttctt gttctaaagt ttgtgtaaca aacatgccat caaagccttc
    27721 taatgtttca atcccatgtc tcgtgtaaaa tcgttctata atatcttttg ctgttccttt
    27781 tgttaacgtc agcctatttt ctgccataaa tttcataatt atcctctttt ctgtttaact
    27841 taccttaatt atttttgcga caacaacaat tcttttcgtt gtttcactat atgcatcttc
    27901 gcacgttgat aaagtcatta ttttatcttt taccgttaca ttaacatctg agttaattac
    27961 agatttacgt tttgtctcat ctaaaaattg ttgataatct tgatcatttt caaaatctgt
    28021 acgtatgtaa ttatctttag tagtagtttt atatgcacta aatacttgca attgatattt
    28081 accatattta ttgtcaaatt caattatctt gtgtttttca taaaaagatt gctttaaata
    28141 atcttctaac acatcaaaca tcgtattatc accgacatgg tgcccgtata aaatagtatt
    28201 atgatttaaa atcttcaatt catttctaaa atccataaaa atactacctt tacgtcgatg
    28261 ttctcgctca aaatctaaat ttaaataatc gtgatttgtc ttaccttgta gtactggata
    28321 attcaatgat gttcctgata attttatcca tccaacaatg tctttattta ttttttcaag
    28381 tgattcaaat tgtggtctca catgtgcttg atgtttgctc atcaacattt gaaatttttg
    28441 ttgtaatttc tcataatttg cgcgttcttg cttgtcttca atatatgttt gaacaatttt
    28501 gtaaccaaaa atgataataa ttacaaccaa taaaatttgt acaatagtta aaaatcgctt
    28561 cattctcata aaaatcctct tttattaacg acgtttcttc agtcatcact aaaccagttg
    28621 ttgtaccgtt ttagattcga tttcgttgac tttgacaaat taagtaaatt agcattggac
    28681 caccgacaat cattaaaata gcattggctg gaatttctaa aggaggctgt atcactcgtc
    28741 ctaataaatc agccactaac aatagccatg caccaataac tgtagaaaac ggaataagta
    28801 ctctgtaatt gcccccaact agctttctaa ccacatgtgg cacaataata cctaaaaagg
    28861 ctagttgtcc aacaatcgca acagttgcac ttgctaaaaa tactgctaat aaacctgtta
    28921 accatctgta acgatcaata ttaaaaccga tacttcgcgc ttgtatgtca tctaaattta
    28981 gtaaattcaa tttaggggac aatagtaatg ttaatattaa tcccaataat gctgatactg
    29041 ctaatatgta tacgtcactc catattttca ttgttaagcc ttgaggaatt ttcattaaag
    29101 ggttttgagt taaaatttct aaaacaccat ttaataatac gaataacgca acacctacta
    29161 atatcatact aacagcattg aatctaaatt tagaatgcaa caatataatt attaaaaatg
    29221 gtattaaacc tccaataaaa cttaataatg gtaagtaaaa gtacaattgt ggaataaaca
    29281 acatacaaag tgctctcatt ataagtgcac ctgatgaaac gccaatgata ttcgcctctg
    29341 ccaaaggatt ttgtagtgct gcttgtaata atgctccaga aactgctaac attgcgccaa
    29401 ccatcaatgc aattaatata cgtggcaatc gcaaatcaat aattgaatcc actgcttcat
    29461 tgctaccagt tgtaaatttt gtaaataggt cattaaatga caatttaatt gtaccggtta
    29521 caaacgaaat ataagccgtt gcgattaaaa tgactaacaa acataaaaat agtagtttct
    29581 ttttattttt tatcatcata tcaactcact attttttatc cttataaaat aaatcatata
    29641 attgagtcat tgccttatca gcatcaacat ttgctgtaat accgaatggc acttcctcta
    29701 agtcataaac atgattattt ttcacagctt taaaatgttt ccaaatatca ttttgtttaa
    29761 attctttttg aaacattttc ttaacttctt caggcattcc gtgtggtaat cgtaaaataa
    29821 tatctggatt gatattcaac aaattttcag tattagacga aatatattga cgatctttca
    29881 ctttaataac attttctcca cctgctattt taactaaatc accaatatat gatttatcag
    29941 ttgctactaa atagctacca ggtacaccca ttaatattaa tactttggga tgtttctttt
    30001 gtttagctgc tttattttca attttttgct ttacagaatt taaatggtca tttaattctt
    30061 ttgcttgtgc tttacgatta aattgatcac ctaattgtgt aatcgacttt tgcatccctt
    30121 ttaaactatc aaaatcataa aagtagcctt tcatatttaa ttgtttgtaa aatggttgca
    30181 tttcatcttt aatcgttgac acactcaaaa catgtgttgg ttttaacttt ttaacagctt
    30241 caacattcgg ctccattggt tgaccaattt ccggtacatc tttataacga ttaggcaatg
    30301 tcttatatga cgtgggtttg ccgacaattg gtaaatccaa tttgtccaat gtcattgtca
    30361 atgcaacagt tgttggtacg attctgaatt cgccggattt agttgattct tgagaactgg
    30421 aagattgaca gctggttaag ataacgacgc ttatcactaa aatggttaaa tactttatga
    30481 ttctcaaact tggttacctc tctttctgct gtataaatgt gccgaaataa gtaaaatggt
    30541 tgctaacaca atgccaactg caaataacat atattgcttt ttatgatttg gttcatcaag
    30601 ttctttaata tcactagata attgactact attcttgcta tcatcatctc tattaaaagc
    30661 tggtaacgct ttttcttttt caactacttt tttcttttcc ttttttgatt tttcatcttt
    30721 atgtttcggt tgctctttgt gattattttc aacctttttt actgttggag ggctattttc
    30781 aacttttaat gtacctgatg ctttaggtcg ctcgttttca atctttttat tactgtcttg
    30841 ttgcacttta tctttttgta caggaatatc atttaatcct ttttcagtaa aaaggctagt
    30901 acgtctttcc ttaatgtctg atttttgact atcttttttg tcatcaatat cttttttaag
    30961 ctgatcttga ttattttctt gattttgatg ttttgaaggc acttcttttg catcgtcatg
    31021 ttgtactttt ttctctttaa aatgaataga atacagcatt tctttgttat ctagatcttt
    31081 cttttcagcc aacgttttaa catgcatatt gattttatcc atatgctgtt caattaaaat
    31141 tcttactatt gtgtcatcct tttcatcttt tataacaaag gggaattctt catttttgtc
    31201 attttgaaaa acaagttgtt ctttatcaat ttgacctttt agttttactt gcaagtaaat
    31261 tttaccagtc atatctgaaa tcaattgcgc cttttcgtca actagttgtt gttcctcagt
    31321 tacattttta ttttcttgat taaaaattag taattctaac gctttattct cttttatctt
    31381 ttcatcaaaa attgatgaag ttaattctgt tttatctttt gcttcaggtt gattaaattt
    31441 ggctgatgga tattttatct catcagccaa cacgctacca atcattgttg tatatgtaag
    31501 aatacctaag ctaataatag ctataataga ttttgtagca atttgtttaa catttctcat
    31561 tattccacat tgcctttaga tttttttcta acaaacaatg tgattgcgat taataatgct
    31621 aaggatgcaa ctggtattgc atatatatat gcaggtgtac cagcatttgt ttgtggattt
    31681 tctactttgt ctttattcga actgttagat tcactttgac cagtagtcgc tccatcagat
    31741 cctttgtcac tacctgaagc agaattttta tcatctttac ctggtgcatt agcacctgct
    31801 acatcagatg gtccattaaa tttatatgta atattgtaat gatggtcata tttgaaaggt
    31861 tttccgttca ctttttcatc gatataaaca tcaattttcc catctatttt accgtttagc
    31921 ttacttactt caaattcaga agttcgttca tctttggcag tatttttact aataattctt
    31981 tctttatgtc cttctatact cattcctgta atccaatgac tgtggtttac agttatttga
    32041 acatacaatt taccattttt cttaatgtac tttgccggtt tattaaaata gtcattagca
    32101 atcgacgtgt cattggtatt gtatttgtaa acctcataat tcaaagtacc gctatctgcg
    32161 gcatttgcag tattactgaa tgtcgcgatg atgataatta acgctaaaat cattgtatta
    32221 aaaactttta aaatattttt caaaacataa tcctcctttt tatgattgct tttaagtctt
    32281 tactaaaatc ataaataata atgattatca ttgtcaatat ttattttata atcaatttat
    32341 tattgttata cgaaaataga tgtgctagta taattgataa ccattatcaa ttgcaatggt
    32401 taatcatctc atataacaac acataatttg tatccttagg aggaaaacaa catgacaaaa
    32461 cattatttaa acagtaagta tcaatcagaa caacgttcat cagctatgaa aaagattaca
    32521 atgggtacag catctatcat tttaggttcc cttgtataca taggcgcaga cagccaacaa
    32581 gttaatgcgg caacagaagc tacgaacgca actaataatc aaagcacaca agtttctcaa
    32641 gcaacatcac aaccaattaa tttccaagtg caaaaagatg gctcttcaga gaagtcacac
    32701 atggatgact atatgcaaca ccctggtaaa gtgattaaac aaaataataa atattatttc
    32761 caagctgtat tgaacaacgc atcattctgg aaagaataca aattttacaa tgcaaacaat
    32821 caagaattag caacaactgt tgttaacgat gataaaaaag ctgacactag aacaatcaat
    32881 gttgctgttg aacctgggta taagagttta actacaaaag tacatattgt cgtgccacaa
    32941 attaattata atcatagata tactacgcat ttagaatttg aaaaagcaat tcctacatta
    33001 gctgacgcag caaaaccaaa caatgttaaa ccggttcaac caaaacctgc tcaacctaaa
    33061 acacctactg agcaaacgaa accagttcaa cctaaagctg aaaaagttaa acctgctgta
    33121 actgcaccaa gcaaaaatga aaacagacaa actacaaaag ttgtaagtag tgaagctaca
    33181 aaagatcaaa gtcaaacaca aagtgctcgt acagtgaaaa caacacaaac agctcaagat
    33241 caaaataaag ttcaaacacc tgttaaagat gttgcaacag cgaaatctga aagcaacaat
    33301 caagctgtaa gtgacaataa atcacaacaa actaacaaag ttacaaaaca aaacgaagtt
    33361 cataaacaag gaccttcaaa agattctaaa gctaaagaat taccaaaaac tggtttaact
    33421 tcagttgata actttattag cacagttgcc ttcgcaacac ttgccctttt aggttcatta
    33481 tctttgttac ttttcaaaag aaaagaatct aaataaatct tcgtcatact cataacttaa
    33541 tatatttttt attttaaatt ttatttaacc tatgtcatag atattacata atctataaca
    33601 taggttattt tttataaaat aacattgcaa ttaactacca tttcaatgta caatacaagt
    33661 aatcaattga taatgattat cagttgataa tatacaatta ggagttgttt ctacaacatg
    33721 aacaaacagc aaaaagaatt taaatcattt tattcaatta gaaagtcatc actaggcgtt
    33781 gcatctgtag cgattagtac acttttatta ttaatgtcca atggcgaagc aaaagcagct
    33841 gaagaaacag gtgttacaaa tacagaagca caaccaaaaa ctgaagcagt tgcaagtcca
    33901 acaacaacaa caactgaaaa agctccagaa gctaaaccag tagctaaacc agtagctaat
    33961 gctgtctcag tatctaataa agaagttgtg gcccctacta ctgaaacaaa agaagctaaa
    34021 gaagttaaag cagttaaaga agttaaagcc cctaaggaag caaaagagga aaaacctgca
    34081 gcaaaagctg acaacaatac atatcctatt ttgaatcagg aactaagaga agcgattaaa
    34141 aaccctgcaa taaaagacaa agatcatagc gcaccaaact ctcgtccaat tgattttgaa
    34201 atgaaaaaga aagatggcac tcaacagttt tatcattatg caagttctgt taaacctgct
    34261 agagttattt tcactgattc aaaaccagaa attgaattag gattacaatc agggcaattt
    34321 tggagaaaat ttgaagttta tgaaggtgac aaaaagttgc caattaaatt agtatcatac
    34381 gatactgtta aagattatgc ttacattcgc ttctctgttt caaatggaac aaaagcagtt
    34441 aaaatcgtaa gttcaactca cttcaataac aaagaagaaa aatacgatta cacattaatg
    34501 gaattcgcac aaccaattta taacagtgca gataaattca aaactgaaga agattataaa
    34561 gctgaaaaat tattagcgcc atataaaaaa gcgaaaacac tagaaagaca agtttatgaa
    34621 ttaaataaaa ttcaagataa acttcctgaa aaattgaaag ctgaatacaa gaagaaatta
    34681 gaagagacga aaaaagcttt agatgaacaa gtgaaatccg cgataactga attccaaaat
    34741 gtacaaccaa caaatgaaaa aatgaccgat ttacaagata caaaatatgt tgtttatgaa
    34801 agtgttgaga ataacgaatc tatgatggat acttttgtta aacaccctat taaaacaggt
    34861 atgcttaatg gcaaaaaata tatggtaatg gaaactacta atgacgatta ctggaaagat
    34921 ttcatggttg aaggtcaacg tgttagaaca attagcaaag atgctaaaaa taacactaga
    34981 acgattatct tcccatatgt tgaaggtaaa actctatatg atgctatcgt taaagttcac
    35041 gtaaaaacga ttgattatga tggacaatac catgtcagaa tcgttgataa agaagcattt
    35101 actaaagcca atgccgataa aactaataaa aaggaacaac aagacaactc agctaagaag
    35161 gaaactactc cagctatgcc tagcaaacca acaacaccac ctgttgaaaa agaatcacaa
    35221 aaacaagaca gccaaaaaga tgacaataaa caatcaccaa gtgttgaaaa agaaaatgac
    35281 gcatctagtg agtcaggtaa agacaaaatg cctgttacaa aaccagctaa agctgaagta
    35341 gaatcaagta gtacaactcc aactaaggta gtatctacga ctcaaaatgt tgcaaaacca
    35401 acaactgctt cttcagaaac aacaaaagat gttgttcaaa cttcagcagg ttctagcgaa
    35461 gcaaaagata gtgctccatt acaaaaagca aacattaaaa acacaaatga tggacacact
    35521 caaagccaaa ataataaaaa tacacaagaa aataaagcta aatcattacc acaaactggt
    35581 gaagaatcta ataaagatat gacattacca ttaatggcat taatagcttt aagtagtatc
    35641 gttgcattcg tattacctag aaaacgtaaa aactaattaa tcgtctttat atttaattat
    35701 taaattaaca aattttaatt ggcagatgag gtattccgat acctcgtccg ccaattattt
    35761 tttgcaatat aaaaagtccc acttaaaaca attattttaa gcgggacttt ttatattgag
    35821 taactaaaat tatttagctg ctacttcttc gccattgtaa gaaccacagt ttttacatac
    35881 acggtgtgat aatttgtatt cgccacagtt tgggcattca gtcatacctg gtactgaaat
    35941 tttgaaatgc gtacgacgtt tgttttttct agttttagaa gttcttcttt ttggtactgc
    36001 catgatatat cctccttaga ttataaacga aaaatactaa atgttagttt aattaacaac
    36061 attatatcat taattaaact acttattgct ctttatcata taattgttgt aatttttgaa
    36121 gccttggatc aacttgtcgt gattctgaat catcttgttc ttgctgttta gcaagctcat
    36181 ctaattgatc ctcatcgatt acttcccaac cattacctac tgtcaacatt tggtcacttt
    36241 gctctgaata agctctcatt ggtttctcaa taataactat atcctcgaca atatcctgaa
    36301 gattaaccat accatcttta ataatgtgat agtgttcatc tacatcatct tgatcatcgt
    36361 tatactgatt gtacccttct aaatcaaata cttctgtagt agttacatct agtgggactt
    36421 taactggtac aagagtacgt gcacaaggca ttgtatacgt tccagtaatg tgaatatccg
    36481 caacgacttc tgttgactta atggttaact gaccttggat tgtaattgga gataaatcaa
    36541 ttaaatctaa tgattctttt aaattgtcaa aactcaccgt ttgatcaaat tcaaatggct
    36601 taccttgata tttccttaat tgcgtaattg accatttcat atggcttcac ctctaacaag
    36661 cacaaaattt attttaactt tctaagaaca agttgtcaag attttttctt aacatatcat
    36721 gttctgttac aatattaata atgaataccg aaaggacgaa ttgcaaatga aaagcgttgg
    36781 cttaatcaca gaatataatc cctttcataa tgggcatcaa tatcatatta atcaatctaa
    36841 aaaacttaca aatgctgacg ttactattgc aataatgagt ggtaactttg tcatgcgtgg
    36901 cgaaccagca atctataata agtttactcg tgcaaaaatg gcattatcaa cagctgattt
    36961 agttatcgaa ctaccagcaa ctgccagttt atcatctggc gatcattttg ccgaactagc
    37021 agttaaagtc gcagattata tgagtgtcga tacaattgca tttggtagtg aaaataataa
    37081 tatcaaaaca ttaaagcaat tagctcacag cattaatgaa attgaacaat ccgaatcctt
    37141 ttcacaaaaa gtaaaagaag gtaaaagcta ccctcgtatc attagcgaat tattagagca
    37201 tcatgaagca ctagcaagtc ctaacaacat acttggtatt agttatctga aagcaattgc
    37261 taaacatgct aaaaacatca atgcaatttc tattaagcga gaaaatgctc aacatcatga
    37321 ttcattaatt caacatcata agtttgcaag tggtacatct attagaacat caatcattag
    37381 tcaagatgat cattggcatc atgtggtacc taaagatatt caacacctgt acgtaacacc
    37441 tcatataaca ttaaatcaaa tattcccata tttgaaatat caaatcatag ctatgactac
    37501 agagtcatta aagaatattt atactgttac tgaaggtttt gaaaatcgtt taaaatctta
    37561 cattaatgaa gcaactgatt ttcatcattt tgtaaaatca ctaaaaacaa agcgttatac
    37621 atatacgcat atccaaagac tgttaatgaa tgtattatta aacattaaac ctacagatgt
    37681 tacgagaaac attcacgcag ttaaagtatt agcaatgaac gaccgtggca gacaatattt
    37741 aaagcattta aaaacagtgt tcccagaaag acaatatata accaatatta acaaaagcaa
    37801 tgcacattac ttcacaaatg aaatcaaagc aacacacata tacaatgcaa taagcgggca
    37861 acaacaaaca gatttcaaca cgcctgtcat acagcaatat cgttaacaaa tgaaaacagt
    37921 attttaggat tgtaaaccat gataaaccta aaatactgtt atttttatta cttaaatttc
    37981 ttcttcaatg ccttttcaac ataaggtgga acgaattcag aaatatctgc tcgataagct
    38041 gcaacttctt taacaatact tgaacttata aatgaataat tagtactaga catcatatat
    38101 aacgtttcaa tttcattgtt caacttttta ttcattgaag ttaagcgtaa ttcatattca
    38161 aaatcactga ccgctcttaa accacgtatg attgttttag ctcctacttg ttcacaataa
    38221 tcgactagta aaccactaaa ttgatgaacc ttgacattag gtaaatgttt aacagattgt
    38281 tcaattaaat ccatacgctc ttctaaacta aacgtacctt cttttttact atttttaaga
    38341 acacagacat gaatttcatc aaatctatct gtacttctct caataatgtc taaatgacca
    38401 taagtaatgg ggtcaaaact acccggaatg accgctattg tatgttccat gctattctcc
    38461 cttttctaat aacaatgtgt ctgtcaaccc ataatggtaa cgtttaatca tattaaacgg
    38521 ttgatagtct atttcttcat gattgctaaa ttcacaaacg atgataccat tttctttcaa
    38581 taaattaaac tctgaaatta gttttaaagc tttatcaatg agacctttat tataaggtgg
    38641 gtctaagaaa atgacatcaa attgaatatc acgttttgac aatgctttta aagctctatc
    38701 tgcattattt ttataaactt cagattgtgc ctctaaatcc aaattcgcaa gatttgattt
    38761 aataactttt acagctttaa aattttgatc aacaaagata accttatcta tacctcgaga
    38821 gagtgcttct attccaagcg ccccgcttcc tgcaaataaa tctaaaccta tacctgacac
    38881 atcatataaa ctattaaaga taccttcttt aactttatcc atagttggtc tcgtattacg
    38941 gccttccata ctttctaaag ctttactttt atgtttacct gcaatgacgc gcatgttgtt
    39001 cacacttcca attcatttag ttatttaata taatttattg agaaaaagga gaatgataaa
    39061 ccaatgaaac aaacatttat tacacttggt gaaggtctaa cagatttgtt cgaattcatg
    39121 acgatgattg aatataacca tcaacgtatt gataaaatta tctattttca ttcaccacaa
    39181 gctgaaaata aaaagtcatc tgtagcaatc attatgaacc ctacaactgg caatcatttc
    39241 caagcatttt atatcatgat taacgctatt aaatatccat atccagattc aaataaaaag
    39301 tttcaaatga ttaatgattg cgctgaaaaa ttcgacatac caattttagg tatcgatgta
    39361 cagcctcctc aagcatttca tgatttatcg ttatattata attatttaat tagtgtgtta
    39421 aggctccaaa aatggatacc agaacttcaa taatattaat tatatatttc gtgtttctct
    39481 ttttcgtaag ttttctttaa gtatttatat ggtgagccat caatgtgtct aacgtattta
    39541 agttgcatta gtttatgtac aatttgatca acatcttgct cattcacata cataattaca
    39601 aatttacgat ctctatttga atgaacgata tgtccatatt ttcggatttg tcgttcatgt
    39661 ttcatatgtt ttaaataaac tacaatacta gttcttggga ttaaattcat ttaaacctca
    39721 ctccatttat tttcagcatt aatcatatca tgtctttatt aattttaaaa tattaagcta
    39781 ccttaattat gtcttatgta attgcacgat aaatgtattt agattatatt aagcatcatt
    39841 atattaattc aattctcaag agcaacacta tatgattaac cgcacatttt gctgtagtca
    39901 gaactatgaa tttgttaagc tataaaagta aacgaggagg aattttttat gaaaaaagta
    39961 aacagaaaca ttttaaatgc ggtactagtc ggtgctggat ttgtaagtag tttgttaatg
    40021 ataaataaaa ataaagtgat tactaaaaag caaaccatcc ctgctttttt caaaggcaat
    40081 gcaccatata tatttgcgca tcgcggtggt atggctttaa gaccagagca aacgcaactt
    40141 gcatttgact atgcaaaaca attaggcgtt gatggttttg aaacagatgt gagactcaca
    40201 aaagatcaac agcttatcgt ttttcatgac gcaactgttg atagaacgac gaacggctct
    40261 ggaaaagtta gtgcccacac attagcagaa ttaaagaaat tagacgcagc ttatcacttc
    40321 aaagatatca atggactaac accatatcgt ggtcatgcac acacagctat actaaccttc
    40381 gatgagttgc tgaagcaata tcctgatatg tatataaacg tagacttaaa ggatgctcct
    40441 gaatcctatg aaggctcaat cgcaccacaa attatgtttg atacaattgc agagaatcaa
    40501 gcatttgacc gtgttcttgt gacaagtttt tataaagaac aaatcgtacg ttttaataaa
    40561 attgcacaaa gatctgttgc aattggtgct agccaacagg aagttacaga ggcattttta
    40621 aaatatcatt tattaggtgg taggtactac caaccattag cacaaacgtt ccaaatgcct
    40681 actcatttta aaggtattaa tttgacttca tctcgtttta tcaaatggtt aaatgatatg
    40741 aatgtcattc caggctatta tggcgtaaat agtatagatc tgatgaatga tttataccaa
    40801 aaaggtgcac acacgattgt gacagatcga ccagatttag cacaacaatt caaacaatca
    40861 ataaacaaca aacaataaac aaaaacatcg agtcagatgc tagtaaatgg tttcaataac
    40921 catctttttc tgactcgatg ttttttataa tgaacaatta caggatccgc ccgtcgcaca
    40981 accactatgt gatgtttgaa agaatggatt gcctgtttct attttgacat gttctgaaac
    41041 agcgtacgca attttcgtaa caacttcatc aattaaatgt tgaagtgcca tttccctagc
    41101 tttgtaatgc atcacaacgt caagcatctc atatgctctt ttgcgttgtc ttgtctctaa
    41161 catgactttc ttataatcag gatgatattt cccgaaacgc attacttcat catatttctc
    41221 ttttgatttt aaaaatgatt gataaagtaa atgggcttcg tcattgtttt cgagcgcctg
    41281 tttagcttgc tcaaatgatg cataaatatc tgatgcaact atcatatcag ccagttcatc
    41341 aatatcatct aatatcgcta gtgaagcttc attaatcata aatacccctc attgttattt
    41401 ttctaagaaa attagtgaat aataatctct cattacatct ccacccatct catcgtattt
    41461 tgaatttaat agacgagatc gatgtatatc tgaattcatc caactatgga ttaatgttgg
    41521 tacatcatta aacgcataac caacattttg agcagttgtt ttataagtaa catgattttt
    41581 atctaattgc cctcttaatg cgtcctctgt aaattcaaca ctatcagaac cattagaggt
    41641 cgcttcatat aagttattag atgcaatatg tgctaaatcg ctattgattt tcaatggttt
    41701 taatcctttt aattttctca tttcattcgt tacttcataa agagaaatta attgatttgg
    41761 attttgctca actggacgct tattatgctc ttctgacgta gaattagaat ttaatcgata
    41821 aggttcaata tctgctaaca tttcttttgt taaaaatcgt acacttagca cctttttcga
    41881 ttgttgatca gaatacactt gtgcatatat gttgccatat ttaatcaatg tttgtgtttt
    41941 taaatcttca tctgaaagtt caaattcata ttttttacca tcaactgtaa aggacggttc
    42001 tggattaata cttgtatgat taaaaatttc tgcagaatgt tgtcctattt ttaacggact
    42061 aacattgaca ttctcacctg tagcatacac tgaaacgatt tcttcacgtt tagttgaaac
    42121 aatgtaataa ctgtttttgt ctttaaacac ataatttttg taaccatctc taaaagggta
    42181 gacacgatct gcttgtccaa atttagaagt aagcacttta atgtctttac ctacccatgt
    42241 accaacacct tctttaggct ttggattttc agcatggtta tttgatttat tcacctgttc
    42301 agatctattt actttattac ctttattcgg gttctctaat acatcaaatt ttaatctcgg
    42361 tgaataaaaa agatatatta aaaatccaac taaaaataat actccgacga ctcttataat
    42421 taactttttc atcaatcaac cacctaaaaa gtattaatac tattgtaaaa aacaacacat
    42481 taattagcaa attttcaaca ctgacataac tgtgtcattt cgatgaaaca aaaccatctt
    42541 caggcacaat gttaacatca taaagatttt ccctacagtc tatatcataa cctatcttta
    42601 tgttttcagg ttgaatttca ttattaggat taaaatatgt ataaaaaaac ggacagttaa
    42661 ggtatcaaat gaataccatc aactgtccga ctattcttca tcaaaaaacc tgataaaaca
    42721 aattgcctta tcagattagt atcatttgta aaagcgtatt aatgaccaaa tgttgcttta
    42781 atcagtgatg tcgtttctcc acctggatat aatacatata ataataagta aactgctaca
    42841 cctgtaattg cagtacagaa ccatataact gaagcgaatg gcccgaattt gcggtgcaca
    42901 ttatatttat ctttaaatgc agtaataatt tgaactagac ctagaatacc accaattgtt
    42961 gctaaattaa tgtggaaaaa taagaaaatt gtataatatt tcttaattga tgctggtccg
    43021 ccaaatgctg tattaccgat aaaaatcgtt ctacttgcat agattaagaa aaaagttaaa
    43081 gcaaaaacag ctgcagctaa cataacattt ttgtgtttat ctatttccct tttccaaata
    43141 agtctccaac caatggcaat taaaattgca ctaatgacaa tacatgtcgt acttatcgtt
    43201 ggtaaaattg gaacgcccat atttttcatc ctaacttaat taatctagat caaagtaagt
    43261 aatgaaacaa tcacagctaa cacgaaaaag atcaccaaat aatttagtga atatataaac
    43321 atttgtgttg cccattttgt ttgatctgaa tttttcttaa atgttgttaa acctaatgca
    43381 atccatccta aatttaataa tgtagctaac actacgaata cgacacctaa atttattagt
    43441 aataaaggca ctggcaataa aataatcaac cagataaaca tactgacacg tgtacgttta
    43501 aagcccttaa ctgatggtaa cattggaata tttgcaagtg catattcatc tttacgttta
    43561 atagctaagg cataaaaatg aattggttgc caacaaaata caactaaaaa cagcgcaatc
    43621 gctgttaaac taatttgtcc ttcaattgca acccatccaa ttagtggtgg tactgctcca
    43681 ggaaaactcc caatcactgt gttccatgtt gtatgtcttt tagaccatat tgagtaataa
    43741 gacacataac ctacaatccc cataagacca agtacgcctg atggtatatt caataaaaac
    43801 aaacaaattt ctccaactaa catcatacca aaacttaaaa gtaataaatt ttgatctgta
    43861 attctattat ttacagttgg tctattttgt ttactaggca taatacgatc aatatcttgg
    43921 tcgtaataat tatttaacgc acatgcgcca cccataatta aagtagatcc taatagcatt
    43981 aataaaattt gaggtattga tgataagaag gaatgatttg tcattacaac tgctagccat
    44041 gcgcccgcaa aagccggaat taagttacct tgaacaagtc ccattttaat tatctgttgc
    44101 aattctttga agttaactct gctaatattt tgtgacaaag tatgctcttt gctcataatc
    44161 cccctcctta aatttgttca tataagatta tgatatctta gattgcataa aaagactagg
    44221 tttaataaaa ttaaattgtg acaaattaac gacaagagaa aatgtcaatt ttgtgacaca
    44281 aataacattt aatttattgc tataatgtat atgttagaaa attttaataa gtagaatcat
    44341 gcatctaaaa gagattaata tttaagcttc aaatttgagt aactgtgact gaaattttat
    44401 agcagtgaac ggcacatcat tgtcgtgtta ttcatatcat tgatgatagt cgatcacttc
    44461 taatactaga aacaattaaa ttatataaac tgtgttggat cccatctctt caaatccaac
    44521 tgttaggttc attcatagta ttaactcaag ttcagctatt aaatcaaaat ctcagtgatg
    44581 cgttttttta tgatggggtg tataaattgt ttggcaaaaa gaatttaaaa tggttaggtg
    44641 tcgtagcaac gttaatgatg acatttgtac aacttggtgg agccttagtt accaaaaccg
    44701 gatcagctga tggttgtggt tcttcttggc cactatgtca tggtgcgttg attccagaat
    44761 tctttcctat tgatacgatt attgaattaa gtcatagagc cgtttcagct ttgtctttat
    44821 taatggtctt atggttagtt atcactgcat ggaaacatat aggctatatt aaagaaatta
    44881 aacctttatc aatcattagt gttggattct tattattgca agcattaatc ggagctgctg
    44941 ctgttatttg gcaacaaaac gattacgttt tagcattgca ctttggtata tcattaatca
    45001 gtttctcatc tgtattttta ataacattga ttattttctc tatagatcaa aaatatgaag
    45061 ctgacgaatt atatatcaaa aagccattaa gacgtttaac atggttaatg gcaatcatca
//
LOCUS       DAMZPP010000003        39108 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMZPP010000003 DAMZPP010000000
VERSION     DAMZPP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 39108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39108
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.003"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..1419
                     /locus_tag="POH73_000119"
     CDS             <1..1419
                     /locus_tag="POH73_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HDI6886108.1"
                     /translation="GRMLPGTKLQAGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLF
                     ESFSGMTATGKLGESEFFDLYSKIVVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDI
                     VELHETGRPVLLITRTAEAAEYFSKVFFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSM
                     TVATSMAGRGTDIKLGEGVEALGGLAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIY
                     ISLDDYLVKRWSDSNLAENNQLYSLDAQRLSQSSLFNRKVKQIVVKAQRISEEQGVKA
                     REMANEFEKSISIQRDLVYEERNRVLEIDDAENRDFKVLAKDVFEMFVNEEKVLTKSR
                     VVEYIYQNLSFQFNKDVACVNFKDKQAVVTFLLEQFEKQIALNRKNMQSAYYYNIFVQ
                     KVFLKAIDSCWLEQVDYLQQLKANVNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRM
                     VKNICQSMITFDKEGMPVIHFP"
     gene            1437..2945
                     /gene="gtfA"
                     /locus_tag="POH73_000120"
     CDS             1437..2945
                     /gene="gtfA"
                     /locus_tag="POH73_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HDI6886109.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFEDQEVIWLYQYFSDISIAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNENGSITYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYTD
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVVAKLAHAIVMYFNNGPQTPHYISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            2938..4296
                     /gene="gtfB"
                     /locus_tag="POH73_000121"
     CDS             2938..4296
                     /gene="gtfB"
                     /locus_tag="POH73_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HDI6886110.1"
                     /translation="MIKLFDYFNGHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFVLFFIKQLEITEHHFVINSLALPFSVLYNLPSNGSDVL
                     VWQEQCDGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVKSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAF
                     ES"
     gene            4538..6421
                     /gene="sasF"
                     /locus_tag="POH73_000122"
     CDS             4538..6421
                     /gene="sasF"
                     /locus_tag="POH73_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HDI6886111.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAVSEKDTE
                     ISKELLSKQDLLDKVDKANRQIEQLKQLSASSKAHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKGSQIEMNGRSDIDSKLDQLLKDLNEVSSKVDRGQQSDEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLSQQIHKSEDSAVSTTDNNHEVAKTPNNDGSG
                     HVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTYHKLNTLQS
                     LDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKHATQRILESIF
                     NKDEADKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQDKSLLISQ
                     ILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKAILNNAKDK
                     KQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLLNLQKRLNQ
                     TKKDIDYVLSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSNLLDSIPDIPTPKPEK
                     TLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIWLSRRNKLK
                     NKA"
     gene            6589..7149
                     /locus_tag="POH73_000123"
     CDS             6589..7149
                     /locus_tag="POH73_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDI6886112.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(7414..9273)
                     /locus_tag="POH73_000124"
     CDS             complement(7414..9273)
                     /locus_tag="POH73_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDI6886113.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSTDDETSKDTTSKDIDKADNNNTSNQDNNDKKVKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKVNQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDNMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            9484..12465
                     /locus_tag="POH73_000125"
     CDS             9484..12465
                     /locus_tag="POH73_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDI6886114.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQEAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVTSTPPSAPNGNTTPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFADITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKTANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     SILKNGKTRIDRLQTVLPSIEQQYISAIKNAQANFPKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFAQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLEPVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(12577..13515)
                     /gene="manA"
                     /locus_tag="POH73_000126"
     CDS             complement(12577..13515)
                     /gene="manA"
                     /locus_tag="POH73_000126"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDI6886115.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLTDMIDNHEFDSLFKRIPVKSGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLAV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(13529..15481)
                     /locus_tag="POH73_000127"
     CDS             complement(13529..15481)
                     /locus_tag="POH73_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDI6886116.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANMIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHILWFIFAVIVGSL
                     VTMITVLLLKRRTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSHLNKPSHVF
                     DQQTIIIADHNMSRNEAIDTLIHQLKVCRYVEHTSHLKNAILEREMESTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWESLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(15558..17432)
                     /locus_tag="POH73_000128"
     CDS             complement(15558..17432)
                     /locus_tag="POH73_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDI6886117.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAEHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDAKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLQMLPKHL
                     STIKVAPLFSEDDRHKIIHVVKQKQNPVQAHHHMDTVNFLVVNTEQKPRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISQKDTAFTKQMMQLIANLDDDSVNHLCSLDNHSLKQQLFEYLQE
                     "
     gene            complement(17672..18145)
                     /locus_tag="POH73_000129"
     CDS             complement(17672..18145)
                     /locus_tag="POH73_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886118.1"
                     /translation="MKKFLITVVIILLIGALGFAIYAAINHSTSSNHNNEQDNKATHQ
                     KKDTEVNHQKDENTNNTNENQNSNNGVTNGNQSSTPSTVQNNGSTAPANTQPSNPNQS
                     NSNQSNSNQGGSSQPSTNNQNHKQQNTPAPNKSNNANTNESSKQASPSQPQSSKN"
     gene            18401..18928
                     /gene="isaB"
                     /locus_tag="POH73_000130"
     CDS             18401..18928
                     /gene="isaB"
                     /locus_tag="POH73_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HDI6886119.1"
                     /translation="MNQTTKLCAATTLALGTLIGATVVGTASPIHQEAQAATTPYYTY
                     HGYIGHNANFIMDKHFINAIKYDNVTFNGIKLAKTNATKKVEKYDQTFKGVTAKGNEA
                     NQLQFIVKNNISLKDIQKAYGKDLKKENNKMKESDSGIFYYQHSKKSLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
     gene            19342..20871
                     /gene="aur"
                     /locus_tag="POH73_000131"
     CDS             19342..20871
                     /gene="aur"
                     /locus_tag="POH73_000131"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HDI6886120.1"
                     /translation="MRKFSRYAFTSMATVTLLSSLTPAALASDTNHKPATSDINFEIT
                     QKSDAVKALKELPKSENVKNHYQDYSVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDEAADKAFNAVKIDKNKAKNLQDDVIKE
                     NKVEIDGDSNKYIYNIELITVTPEISHWKVKIDADTGAVVEKTNLVKEAAATGTGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAYNFNDQTGQATLITNEDENFVKDDQRAGV
                     DANYYAKQTYDYYKNTFGRESYDNHGSPIVSLTHVNHYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTNLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPSHMKDYVYTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            21238..21687
                     /locus_tag="POH73_000132"
     CDS             21238..21687
                     /locus_tag="POH73_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDI6886121.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFKHYCEHEVLSSIINGAYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            21690..21857
                     /locus_tag="POH73_000133"
     CDS             21690..21857
                     /locus_tag="POH73_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886122.1"
                     /translation="MYQMNNKNLFCITCTQVSYYILTCVRVFLWKERIHSHSFDCIKN
                     FIQHVFVGVFE"
     gene            22034..23269
                     /gene="arcA"
                     /locus_tag="POH73_000134"
     CDS             22034..23269
                     /gene="arcA"
                     /locus_tag="POH73_000134"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDI6886123.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKEVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTIHSAILKAEGNMNIFIIEYDDVNKDISIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            23302..24312
                     /gene="argF"
                     /locus_tag="POH73_000135"
     CDS             23302..24312
                     /gene="argF"
                     /locus_tag="POH73_000135"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDI6886124.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKDAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQLFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            24403..25833
                     /gene="arcD"
                     /locus_tag="POH73_000136"
     CDS             24403..25833
                     /gene="arcD"
                     /locus_tag="POH73_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDI6886125.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSIIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFGMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLASIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYSIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            25850..26791
                     /gene="arcC"
                     /locus_tag="POH73_000137"
     CDS             25850..26791
                     /gene="arcC"
                     /locus_tag="POH73_000137"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDI6886126.1"
                     /translation="MKEKIVIALGGNAIQTKEATAEAQQTAIRRAMQNLKPLFDSPAR
                     IVISHGNGPQIGGLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPGSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFAEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            26890..27594
                     /locus_tag="POH73_000138"
     CDS             26890..27594
                     /locus_tag="POH73_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDI6886127.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLLCHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
     gene            27944..30565
                     /gene="clfB"
                     /locus_tag="POH73_000139"
     CDS             27944..30565
                     /gene="clfB"
                     /locus_tag="POH73_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HDI6886128.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNTIETPQLNTTANDTSDISANTNSANVDSTAKTMST
                     QTSNTTTTEPASTNETPQPTAIKDQATAAKMQDQTVPQEANSQVDNKTTNDANNIATN
                     SELKNPQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASDFKLEKTAFDPNQSGNTFMAANFKVTGQVKSGDYFTAKLPDSVTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNDKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFDNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADP
                     NDSNLKEVTGEFKDKISYKYDNVASINFGDINKTYVVLVEGHYDNTGKNLKTQVIQEN
                     IDPATGKDYSIFGWNNENVVRYGGGSADGDSAVNPVDPTPGPPVDPEPEPEPTPDPEP
                     SPEPEPEPTPDPEPSPEPDPDSDSDSDSGSDSDSGSDSYSDSDSDSDSDSDSNSDSES
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSNSDSDSDSDSDSDSDSDSDS
                     DSNSDSDSDSDSDSDSDSDSDSDSDSESDSYSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSESDSESDSDSDSESDSDSDPDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDS
                     DSRVTPPNNEQKAPSNPKGEVNHSNKASKQHQTDALPETGDKSENTNATLFDAMVALL
                     GSLLLFRKRKQDHKEKA"
     gene            30681..31442
                     /locus_tag="POH73_000140"
     CDS             30681..31442
                     /locus_tag="POH73_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDI6886129.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNNDTTAKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRKNVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            31687..32142
                     /locus_tag="POH73_000141"
     CDS             31687..32142
                     /locus_tag="POH73_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDI6886130.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(32245..32355)
                     /locus_tag="POH73_000142"
     CDS             complement(32245..32355)
                     /locus_tag="POH73_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886131.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(32425..33849)
                     /locus_tag="POH73_000143"
     CDS             complement(32425..33849)
                     /locus_tag="POH73_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDI6886132.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSADVEKQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            34047..34247
                     /locus_tag="POH73_000144"
     CDS             34047..34247
                     /locus_tag="POH73_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886133.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQAKEQKDNKG"
     gene            34273..34938
                     /gene="nsaR"
                     /locus_tag="POH73_000145"
     CDS             34273..34938
                     /gene="nsaR"
                     /locus_tag="POH73_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HDI6886134.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQHQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQSIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            34949..35836
                     /gene="nsaS"
                     /locus_tag="POH73_000146"
     CDS             34949..35836
                     /gene="nsaS"
                     /locus_tag="POH73_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HDI6886135.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQFNQLYVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            35944..36699
                     /locus_tag="POH73_000147"
     CDS             35944..36699
                     /locus_tag="POH73_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6886136.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLTVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMKLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLVFQQRITDSLALVNGGSVNI"
     gene            36696..38696
                     /locus_tag="POH73_000148"
     CDS             36696..38696
                     /locus_tag="POH73_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6886137.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFMIISVISIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDTVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTIMKLITNDYDLSFNDNSKSQ
                     IERQQTIEHIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGSIFSKQSVMITALTVQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKKSTISINFNVSGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRISKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMLG
                     INNHHIFLTCYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
BASE COUNT        13768 a         5812 c         7181 g        12347 t
ORIGIN      
        1 ggtcgtatgt taccgggaac taagttgcaa gctggactcc accaagctat tgaagcgaaa
       61 gaaggtatgg aggtttcaac agataaaagt gttatggcaa caattacctt tcagaattta
      121 tttaaacttt ttgaatcatt ttcaggtatg acagctacag gaaaattagg cgaatcagag
      181 ttctttgatt tgtattcaaa aatagtcgta caagtaccaa ctgataaagc gattcaacgt
      241 atcgatgaac cagataaagt gtttcgttca gttgatgaga aaaacatcgc aatgattcat
      301 gatatagttg aacttcatga aacggggcga ccggttttac tcataacgag aactgctgaa
      361 gcggctgaat acttttcaaa agtatttttc caaatggata ttcctaataa tttactcatt
      421 gcgcaaaatg ttgcaaaaga agcgcagatg atagctgaag cagggcaaat tggttccatg
      481 actgttgcga ctagtatggc aggtcgaggc acagatatta aacttggtga aggtgtcgaa
      541 gcattaggtg gattagctgt tattattcat gaacatatgg aaaatagccg tgtagacagg
      601 caattacgtg gtcgttctgg tagacaaggg gatccaggat catcttgtat atatatttca
      661 ctagatgatt atttagttaa gcgatggagc gatagtaatt tagcggaaaa taatcaatta
      721 tattcattag atgcacaacg attatcgcaa agtagtttgt ttaatcgcaa agttaagcaa
      781 attgtagtta aagcgcagcg tatctcggaa gaacaagggg ttaaagctcg tgaaatggct
      841 aatgaatttg aaaaaagcat tagtatacag cgagatcttg tatacgagga acgcaatcga
      901 gttttagaaa tagatgatgc tgagaatcga gattttaaag tgttagctaa agatgtgttt
      961 gaaatgtttg taaatgagga aaaggtgcta acaaaatcgc gtgttgtcga gtatatttat
     1021 caaaatttaa gtttccaatt taataaagat gtggcttgtg ttaattttaa agataagcaa
     1081 gctgtagtaa catttttatt agagcaattt gaaaagcaaa tagctttgaa tcgtaaaaac
     1141 atgcaaagtg catattatta taatattttc gttcaaaaag tctttttaaa agcaattgat
     1201 tcatgttggt tagaacaagt cgactattta caacaattaa aagcaaacgt taatcaacga
     1261 caaaatgggc aacgtaacgc tatttttgaa taccatagag ttgcattaga ttcatttgaa
     1321 gttatgacac gaaatatcaa aaaaagaatg gtgaaaaata tttgtcaaag catgattaca
     1381 tttgataaag aaggtatgcc agttatacat tttccataaa cgaaaagggg tagcgcatga
     1441 caatatataa tataaacttt ggtataggct gggcgagtag cggtgttgaa tatgcacaag
     1501 cttaccgtgc taaattattg agacgcacaa atcaagacac taaatttgtg tttttagatt
     1561 ttatacagtc agagaacata cagacattaa cgcaaaacat tgggtttgaa gatcaagaag
     1621 ttatttggtt gtatcagtat tttagtgata tttctatagc accaacaacg tacacgttag
     1681 atgacattga acgtgagctt ggtcatgata ttagttttag agaaagaaat ggtaagatag
     1741 tccgcctata ttttaatggg aaatcatcat ttgtaacatg ttatctgcaa aatgaacaaa
     1801 aagacattgt ggatcgtgca gaatttgtaa ttaatagtat gttagtacgt aaagattttt
     1861 atagttatac acgcatattt tctgagtatt atgcaccagc tgataataaa gcgaaattat
     1921 atatgcgaca attctataat gaaaatggct ccatcactta taacgaatat attgatggag
     1981 acagctctat atatgtgttt gaagatgcgg tgttgtataa caaaacggaa tttattgcat
     2041 actttttaca acgattgaat ttaacccgag acgatattgt catattagac cgcgcttctg
     2101 atattggtca ggctgttttg cagcataaag gtgatagtaa agttggtgtt gtgattcatg
     2161 ctgatcatta tagtaataat atgatgagcg agcagcatat actatggaac aattattatg
     2221 aatatcaatt ttcaaaagct aaatacattg atttctttat tacagcgaca gatattcaaa
     2281 atcatatggt ttgtcgacaa tttgagcaat atcaaggtta tcgcccacgt gtttatacta
     2341 ttccggtggg aagcattgat gcattatcat atccaacatt atcaagaaag ccatacgcga
     2401 tgattagtgc atctcgttta gcaaatgaga aacatattga ttggcttgtc aaagcagtta
     2461 tcgtggctaa acgacaagtg ccagaattaa catttgatat ttatggagaa ggtagtgaga
     2521 agacgcgact gcgtaaaatt attgatacac accgcgcgca agattatatt cgtttactag
     2581 gtcatgttaa acttgatgaa atttatactg attatgagtt atttctttct gcatcaacta
     2641 gcgaaggttt tggattaaca ctaatggaag cggtaggctc aggactaggt atgattggat
     2701 taaatgtgaa ttatggtaac ccaaccttta ttcgagatgg ggaaaatggc tacttggtac
     2761 cttttgatac cgatgaagat agtgtggatg atgttgtcgc aaaactagct catgccattg
     2821 taatgtattt taacaatgga ccccagacac cacattacat ctcttatgaa gtggcacagc
     2881 aatttatgac tcaagatatt attttaaaat gggaaacatt agttcaagag gtgctgcatg
     2941 attaaattat ttgattactt taacggtcat tcacgaaagt tatatgagtc ttttaaagcc
     3001 agtaagttgg aggaagattt aacaattgta ctcaatgaca atggtttttt accggacgat
     3061 gttatatcac cgtatcaatt ttttgcagat aatcataata cagaaaatat gaaaccacga
     3121 ttttttaatc aggttactgt accagctttt tgggaaatta aaggtaacaa caattcggct
     3181 acaattaatg atatgggacg tctacgaggc aaaatatttt atcaaagtgg agagcgccct
     3241 agaattgtta gtcgtgtcga atggtttgat gaccaacaac gtgtccgatt tgtcgattat
     3301 tattctaaga atggtattaa atttgcacaa actgtttacg atttaaatcg taaagcgatc
     3361 ttgaagaaat atatgacagc agaaggtaaa gaagtgattt atgaaaattt tgtaacatct
     3421 gatgtcatat tggattggca aggaaagtct tactttttcc cttcaaagct cgcattcgta
     3481 ttatttttta tcaagcaact tgaaattacg gaacatcatt tcgttattaa ctcactagcc
     3541 ctaccatttt cagtgttata taatttgcca tcaaatggta gtgatgtttt ggtatggcaa
     3601 gaacagtgcg atggtaatgt cccgggaaat atgcaattga tgtgtaaagg tgatatgaaa
     3661 cgacattgta atattattat tccagataaa aacgaatatg aaacgatgct caatatagca
     3721 gacgcaaagg ttaaatctcg tattttacaa ggcggatatc tatataatta tcgtagtcgt
     3781 aatagatata ctaaagaaat tgtaatatta actaactcgg atcaacttcg taacatcaag
     3841 gtattggttg aaactttacc agattttaag tttcacatcg ctgctattac agagatgtct
     3901 gataagctaa tgcaacttga tcaatatgct aatgtgcact tatacccatc tattaatata
     3961 gacagggtaa atgaactata ccagttatgt gatatttatc ttgatattaa tgagggtaat
     4021 gaaattttaa atgcggtgga acaagcgttt gactatgagt tgttgatttt aggttatcgt
     4081 caaacagcac accatgctaa agtaacacta tcagagcact tatttgagca taatgatgag
     4141 ataacaatgg aaagtaaaga ccaattaata caaatgttag aaagtttgaa agatcaacaa
     4201 cagtttcgag atgctttatt agcacaaaaa gcacatgcac acgaaatctc gcgtgaacaa
     4261 ttcgagcaag tttttaaaca ggcgttcgaa agctaaaaag aaaaagcagt ctaaaataaa
     4321 acatcattta atataaaaga tttgacgtaa tatgatgtta gcgacatgga caatatgata
     4381 ttacgtcatg cttgttttgt ttacttaaat ataagctaag taataagtag ataattacta
     4441 acaacaataa ctagatagat aagtgtaaat ttcttgtaaa caggtatata atagtatgta
     4501 attcttttaa ggaagtaagt gagaaaggag tatgttgatg gctaaatatc gagggaaacc
     4561 gtttcaatta tatgtaaagt tatcgtgttc gacaatgatg gcgactagta tcattttaac
     4621 gaatatcttg ccgtacgatg cccaagctgt atctgaaaag gatactgaaa tttcaaaaga
     4681 gctattatct aagcaagatt tattagacaa agttgacaaa gcaaatcgtc aaattgagca
     4741 attaaaacag ttatcggctt catctaaagc acattataaa gcacaactaa atgaagcgaa
     4801 aacagcatcg caaatagatg aaatcataaa acgagctaat gagttggata gcaaagacaa
     4861 taaaggttct caaattgaaa tgaacggtcg aagtgatata gacagtaaat tagatcaatt
     4921 gcttaaagat ttaaatgagg tttcttcaaa ggttgatagg ggtcaacaaa gtgacgagga
     4981 cgatcttaat gcaatgaaaa atgatatgtc acaaacggct acaacaaaac atggagaaaa
     5041 agatgataaa aatgatgaag caatggtaaa taaggcgtta gaagacttag accatttgag
     5101 tcagcaaata cacaaatcgg aagattcagc agtgtctaca acagataata atcatgaagt
     5161 agctaaaacg ccaaataatg atggttctgg acatgttgtg ttaaataaat ttctttcaaa
     5221 tgaagagaat caaagccata gtaatcgact cactgataaa ttacaaggaa gcgataaaat
     5281 taatcatgct atgattgaaa aattggctaa aagtaatgcc tcaacgcaac attacacata
     5341 tcataaactg aatacattac aatctttaga ccaacgtatt gcaaatacgc aacttcctaa
     5401 aaatcaaaaa tcagacttaa tgagcgaagt aaataagacg aaagagcgta taaaaagtca
     5461 acgaaatatt attttggaag aacttgcacg tactgatgat aaaaagcatg ctacacaacg
     5521 cattttagaa agtatattta ataaagacga ggcagataaa attctaaaag atatacgtgt
     5581 tgatggtaaa acagatcaac aaattgcaga tcaaattact cgtcatattg atcaactatc
     5641 tctgacaacg agtgatgatt tattaacgtc attgattgat caatcacaag ataagtcgct
     5701 attgatttct caaattttac aaacgaaatt aggaaaagct gaagcagata aattggctaa
     5761 agattggacg aataaaggat tatcaaatcg ccaaatcgtt gaccaattga agaaacattt
     5821 tgcatcaact ggcgacacgt cttcagatga tatattaaaa gcaattttga ataatgccaa
     5881 agataaaaaa caagcaattg aaacgatttt agcaacacgt atagaaagac aaaaggcaaa
     5941 attactggca gatttaatta ctaaaataga aacagatcaa aataaaattt ttaatttagt
     6001 taaatcggca ttgaatggta aagcggatga tttattgaat ttacaaaaga gactcaatca
     6061 aacgaaaaaa gatatagact atgttttatc accaatagta aatcgtccaa gtttactaga
     6121 tcgattgaat aaaaatggga aaacgacaga tttaaataag ttagcaaatt taatgaatca
     6181 aggatcaaat ttattagaca gtattccaga tatacccaca ccaaagccag aaaagacgtt
     6241 aacacttggt aaagggaatg gattgttaag tggattatta aatgctgatg gtaatgtatc
     6301 tttgcctaaa gcgggggaaa cgataaaaga acattggttg ccgatatctg taattgttgg
     6361 tgcaatgggt gtactaatga tttggttatc acgacgcaat aagttgaaaa ataaagcata
     6421 attatattgg gggaagtgca tctatatatt ttttaagtat ataagacgtc ttatttcccc
     6481 cttaaattat tgtgaagtat atgcaaaatg caatgaatag attgtccatc attttaacgt
     6541 tataatgaat ttaacgactt agaactacac aagtaaagga gaatgaagat gtctcgaaaa
     6601 acggcgctat tagttttgga tatgcaagaa ggtatagcga gtagtgtacc tagaataaag
     6661 aatattatta aagcgaatca gagagcaatt gaagcagcaa gacaacatcg aataccagtc
     6721 attttcatac gtttagtgtt agataagcat tttaatgatg tctcctcgag taataaagtg
     6781 ttttcaacaa ttaaagctca aggatatgcg attactgaag cagatgcatc tacacgaata
     6841 cttgaagact tagcaccact agaagatgag ccgattattt ctaagcgacg ctttagcgca
     6901 tttacaggta gttacttgga agtttattta cgtgcaaatg atattaatca tttagtatta
     6961 acgggtgtct ctacaagtgg agctgtattg agcacggcat tagaaagtgt agataaagac
     7021 tattatatta ctgttttaga agatgctgtt ggtgatagat cagatgataa acatgacttt
     7081 attattgaac aaattttatc acgctcatgt gacattgaat ccgtagagtc atggaaaagt
     7141 agtttatagt aaatataacg tcaattaaag ctcggcagta atgtttgaga ataagtacat
     7201 ttgctcatat ttataaaatg tgtgagatgg caattgaaac ggatatgttg aggaacatta
     7261 gaacataaaa taatatattt atataaaatg acccgaggcg ttcgaactga atgcctcggg
     7321 tttaattgaa taagaaatcg gacttatgaa cagaaatatg tttaagtccg aactccttgt
     7381 ttatacttat aaattttacg ggtttaataa tatttattta cctgtaatat atgataattc
     7441 ttcagcggca gctgcattga tagttctatg agaaatgata cctaatcctt taacattgga
     7501 ttctgaaata acgatagaac catcactgtt aactttttca acaaatgcta catgaccgta
     7561 atgttgatct gcaccaaatt gtccagcctc aaatacaaca gcagcatgac gttttggtgt
     7621 atgacttact tgataatcac ggtattgagc tcgattattc caattatgtg catcacctaa
     7681 atcacctgag atagatgtac caaattgttt catacggtta tatacgtacc aagtacattg
     7741 gccatgtgga tatggcatat tatcagatac ctcgcggaaa ggtttgaatt catctgatga
     7801 atcatcataa tccttgatag aacgttcata tttatctaaa tctggcatac gttcatcgtc
     7861 aaactgagtt aattgatagt gtttaataat actgtttaat ttcttagcat agtttggatc
     7921 tgtagcatat gttttagata agtgtgatgt tgcatcttta taagaatcgg cttccgattt
     7981 ccatgttggt ttataaattg ttcgattgcc atcaatacca tttttaataa ggtcagagta
     8041 atcttttagt gattctttcg tgcttggata ttttcggaat ccagcattaa tactatacaa
     8101 ttgattacca tcagcttcta atgtgttaaa aggaacagaa ttcccttcaa aagcaccttt
     8161 gataccgaat aaattatggt ttggtgactt agctaaagca ctacgacctg agtcagattc
     8221 taagattgct tgggcaatca tgacagacgc ataaatatcg ttatcttgac caatgcggtg
     8281 tgcatcttta gcaattgatt tgacaaattg acgtgtatct tttgagtcaa caacgttaaa
     8341 ttgtccacta tcatcattgt tagatatact aggatctgtt tcgaatagtg atgttgcacg
     8401 tgtatccttt tgattaacat cgttattgaa tgattgagca ggtttagatt tatgtttcaa
     8461 ttcatcttgt gttggtaact gtggattctt tgtattagat ttttcatttt tgtctttttt
     8521 agattgagat gcgtaatctt tttgtgtttt ctttgcatct tcactgtatt gatccaaaat
     8581 agaatctaaa gccgaatctg acattgattg attatctttc gatgaagatt tttgatttac
     8641 tttatcgtca cttgctggtt gactatttga ttgatttggt tgtgttggct ttggcgaatt
     8701 tggttgctta ttagatgtac ttggttttgt attgtttgat ttaggtgctt tttgattgtc
     8761 tgctttatct tgtttagatg attgcgtatc cgtatcattt ttgatgctat tatcactgtt
     8821 tttattcgaa tcatttgttg acttttcacc attacgaggt tgttcgtaat cagaaatatc
     8881 cgaatttaaa ttgaataagt tttggattaa agttgttaat gagtaattat catcgtattt
     8941 atttttggtt agcaattggt ttatattggt ttgtggtaaa ttcttataaa taaaatcaat
     9001 gatattgtta gagtctgaag tgctgtcgtc tatagtttta acttttttgt cgttattgtc
     9061 ttggttacta gtattattgt tgtctgcttt atcaatatct ttacttgtag tatccttaga
     9121 agtttcatca tcagtagatt ttttggaatc atgagatgtt gtcttagctg tagtatcttt
     9181 ttgaggtgtg tcagcataag cggtaggtga aactaaagta ggtaatacga gcgtagttga
     9241 tagcaaataa attaaaattt tatttttagg catatttcgt attctccctt gaaaaatata
     9301 ataattaagt gtgataataa actatgattt gttataattt atcgtatgct gaaaatagtt
     9361 gataggtatc aatcgactaa atatcttcca gtaaattgat tatactaatt cacaacgcaa
     9421 aaataaatta atttacaaaa aatatataaa aaatatgaat aattcctaca taggagtgtg
     9481 acaatgaaga acgcatttaa attatttaaa atggatctga agaaagtagc taagacgcca
     9541 gctgtgtgga ttatcttagc aggcttagct attttgccat cgttctacgc ttggtttaac
     9601 ttatgggcaa tgtgggatcc atatggcaac acgggacaca tcaaggtcgc agtcgttaat
     9661 gaagataaag gcgacacaat cagagggaaa aaagttaatg tcggtaatac gatggttaac
     9721 acactcaaga aaaataaaag ttttgattgg cagtttgtaa gtagagagaa agctgatcat
     9781 gagataaaaa tgggtaaata ttttgcaggt atttacatcc catctaagtt tacacatgaa
     9841 attaccggga cactacgtaa gcagcctcaa aaagcagatg tagaatttaa ggtgaatcag
     9901 aagattaacg ctgttgcgtc taagctaaca gatactggtt cgtcagttgt cgttgaaaaa
     9961 gcgaatgaac aatttaataa aacagtaact cgagcattat tagaagaagc taacaaagca
    10021 ggtttaacta ttgaagaaaa tgtgccgaca attaataaga taaaaaatgc ggtatattcg
    10081 gcagataaag ctttacctaa gattaatgac tttgcgaata aaattgtata tttgaataac
    10141 caccaagcgg atttagataa atatgcgaat gattttagaa aactaggaaa ttataaaggt
    10201 gatattttag atgctcagaa aaaattaaac gaagtcaatg gtgctattcc gcaacttaat
    10261 gaaaaggcta agttgatatt agctttaaat aattatatgc cgaaaattga aaaagcgtta
    10321 aattttgcag ctgatgacgt gccagcgcag ttccctaaaa ttaatcaagg acttaacatt
    10381 gcgagtcaag gtattgatca agctaatgga cagttaaatg atgccaaagg cttcgtcaca
    10441 caagttagaa gtagagtcgg tgattatcaa gaagcaattc gacgcgcgca agatttgaat
    10501 cgaagaaacc agcaacagat tcctcaaaat agcgcggcga acaacgaaac atcaaatagt
    10561 gcacctgcag ctggtaatgg tgtaacatca acgccaccaa gtgcaccaaa tggcaatact
    10621 acaccaaata ataatgttac gcaaaatacc gcaccaaata gtaataatgc acctgtatcg
    10681 actacaccac aaagtacaag cgggaaaaaa gatggtcaaa gttttgcaga tataacaaca
    10741 acacaagtca gcacagctaa cgagaacaca caaaatatta cagataaaga tgttaaatca
    10801 atggaagcgg cattaacggg ctctttatta tcattatcaa ataatttaga tacccaagcg
    10861 aaagccgcac aaaaagatag tcaggcatta cgtaatattt cgtatggcat tttagcatcg
    10921 gacaagccat ctgattttag agagtcttta gataatgtta agtccggttt agaatacaca
    10981 actcaatata atcaacaatt tatcgataca ttaaaagaga ttgagaagaa tgaaaatgtt
    11041 gatttatcaa aagaaattga taaggtaaag acagctaata atcgaattaa tgaatcatta
    11101 aggttagtta atcaattaag taatgcatta aagaatggta gttcaggaac tgctgaagct
    11161 actaaattac tagatcaact gtcaaaacta gattcatcat tatcatcatt tagagattat
    11221 gttaaaaaag atcttaacag ctctttagta tcaatatcac aacgtattat ggatgaattg
    11281 aacaaagggc aaacggcatt gtctaatgtt cagtctaagc taaatacaat tgatcaagtc
    11341 atcaacagtg gtcaatctat tttaaaaaat ggtaaaacac gtatcgatcg tttacaaaca
    11401 gtattaccaa gtattgaaca acaatacatt agtgctatta aaaatgctca agcaaacttc
    11461 ccgaaagtga aaagtgatgt agcgaaagca gctaactttg tacgtaacga tttaccacag
    11521 ttggagcaac gtttaaccaa tgcgactgca agtgtgaata aaaatttacc aacgttatta
    11581 aatggttatg atcaagcggt aggattacta aataaaaatc agccacaagc gaaaaaggct
    11641 ttatcagatt tagctgattt tgcacaaaat aaattgccag atgttgaaaa agatctgaaa
    11701 aaagcgaata agattttcaa gaagttagac aaagacgatg cagtcgataa attaatcgac
    11761 acacttaaga atgatttgaa aaagcaagcg ggtattattg caaatcctat taataagaag
    11821 actgttgatg ttttcccagt taaggattat ggatcaggga tgacaccatt ctatactgca
    11881 ttatcggtat gggtaggcgc actcttaatg gtaagcctat taactgttga taataaacat
    11941 aagagcttag agccagtgtt aacgacacga caagtattct taggtaaagc aggattcttt
    12001 ataatgcttg gtatgttgca agcactcatt gtatcggttg gagatttgtt aatcctaaaa
    12061 gcaggagttg agtcacctgt attattcgta cttataacga ttttctgttc gattattttc
    12121 aactcaatcg tatatacgtg cgtatcatta cttggtaacc caggtaaagc cattgcaatc
    12181 gtattgcttg tattacaaat tgcaggtggt ggaggtacat tcccaattca aacgacacca
    12241 caatttttcc aaaacatttc gccatactta ccatttacgt atgcaattga ttcattacgt
    12301 gaaacagtag gcggtattgt tccggaaatc ttaattacaa aattaattat attaacgtta
    12361 tttggcatag gattcttcgt tgtaggttta attttaaaac ctgtaacaga tccattgatg
    12421 aagcgcgtat ctgaaaaagt tgaccaaagt aacgttacag aataaaaatt aaatccacac
    12481 attagggtta tagctcctta atgtgtggat ttttatgttt ttagacagaa gagatagtaa
    12541 tttctgtctt ttatgggacg ggtgttatca ttgctattat ccaggatgac ttactatagc
    12601 actaatatta ccgacaaagt gaatatcctc atcttccgta gttaaaataa agctagaacc
    12661 tttttggatg tcatagtgct tatcgtttac tgctaaagtg ccagtaccat cgataattgt
    12721 aactaagcaa taagcatgtg gtttattgaa ttttaaatct ccatgaatat cccatttata
    12781 tactgcaaaa tattgattat ctacaaattg agttacagtg tgtgtgtcga tgtgagttgt
    12841 tataggagta gtatttggtt catgattgcc taattcaatc acatctttac tttgctctaa
    12901 gtgcaaatca cgcaattgac cattttgatc tcgtctatca tagtcataaa tacggtatgt
    12961 cgtatcggat gattgttgtg tctctaaaat taaaataccc gaaccaatgg catggacagt
    13021 gccagcagga acataataaa agtcaccgga cttaacaggt atacgtttga aaagactgtc
    13081 aaattcatga ttatcaatca tgtcggttaa cgtttgttta ttatgtgcat gtacgccata
    13141 tataatttca gcacctgggg ctgcatctaa aatataccaa cattctgttt tacctagttc
    13201 gccttcgtgt tttaaagcgt aatcatcatc cggatgaact tgaacagata atttatcatt
    13261 ggcatctaat actttagtta gcagagggaa actatctcgt gaatcattat cgaataattc
    13321 acgatgttgt gaccaaagtt gatctagggt catatccttg tatggaccat tgataattgt
    13381 attaggacca tttggatgtg cagaaattgc ccagcattca ccagttgttt cattagggat
    13441 atcatagtta aatgctttta atgcatgacc gccccaaatt ctgtctttaa aaacgggttg
    13501 taaaaataat gccatagtta aaactcctct atattttcat taataagtta taaatttctg
    13561 tagtactgtt tgcattaatt agtgattggc gcgtctcatc attcattaac gctttagata
    13621 agcgctgaag tatttttaaa tgtgtatcct gactgttgtt tggtacggca attaagaaaa
    13681 tcaattgagg tagactacca tctagacttt cccatttaac accatgatta tttttcataa
    13741 cagctacaat cggttgtttt acaacatcag actttgcatg tggaatggcc acgttcatgc
    13801 caatagctgt cgtagactcc atttcacgtt ctaggattgc attttttaaa tgcgatgtgt
    13861 gctctacata acggcacact ttaagttgat gaatcaacgt atcgattgct tcgtttcgag
    13921 acatgttatg atcagcaata atgatagttt gttgatcaaa cacatgagaa ggtttattga
    13981 gatgtgaatg tttcgcggtg ttatctacat tgtcaacctc tgtatcatgt tgtgttatat
    14041 ctgtatcatg aagttgcgta tgttgcgctg gtgcatctac tgctataact ggtgtgcgac
    14101 gttttaataa tagtacagta atcattgtga caagactacc tactatcact gcaaagataa
    14161 accataatat atgatcaata ccacctaata cagccactat tggacctcca tgtgccactc
    14221 tatctccaac accaccgatg gctgcaatga ctgatgcaat catcgctcca atcatgttag
    14281 caggtataat gcgtagtggg tcttgagctg cgaaaggaat agcaccttca gtaataccaa
    14341 atagtcccat agtgaaggaa gccttaccca tttctctttc ggaatgattg aatttatact
    14401 tttgaacaaa cgttgctaaa ccaagaccga ttggtggtgt acatacagca actgcgacca
    14461 tacccataac ggcgtaatta ccttcagcaa taagtgctga gccaaataaa aatgctactt
    14521 tgtttactgg accgcccata tcgaaggcga tcatcgcacc tattatcatc gcaagtataa
    14581 taatattagc accttgcata ctttttaacc aggttgttaa tgcctcaaaa atattagaaa
    14641 ttggtgcacc gattaaaaat ataaatatca atcctacaac gaccgatgaa ataatgggaa
    14701 taataatgat aggcataatt ggtgccatcg cttttggaac tttaatatct ttaatccact
    14761 ttgcgatata acctgctaag aaaccagcaa caataccacc taaaaatcct gcgcctgcat
    14821 cactgccata aaaactaccg tcagcagcga tagcgccgcc aatcatacca ggaacaagac
    14881 cgggcttgtc agcgatacta acagcgatat atccagctag tattgggacc ataaatttaa
    14941 aggctaaact accaatgttt tcaatggatt tccaaaatga atcatccggt atgactaatc
    15001 cttttgatgt cgtttcaccg cctagagtca gcgcgatggc gataaggagt ccaccaacta
    15061 cgataaaagg aaccataaac gatacaccgt tcattaaatg ttgatacacc atttgaatac
    15121 cacttttaga cttaccgcga tctttcgaat gataatttgt ttcagattga taaataggcg
    15181 catcttgatt aatgatacgt tgaattagac ctctcggatt atgaatccct tcgcgaacat
    15241 tttcattaat caaccgttta ccaacaaatc gggacagatc aacttgttta tcagctgcaa
    15301 ttatgacacc gtcagcttct tcgatgtctt gcgtagttaa aacattttca gcaccaacac
    15361 cgccctgtgt ctctacttta atatccacac ccatttcttt tgctacctgc tcaagttttt
    15421 cttgagccat atatgtatgt gcaatgccat ttgggcatga ggtaatagct acaattttca
    15481 taaaatcatc tccttttcca tattgtaagc gtattcttga tgctaaaaaa aagaataatt
    15541 accgttacta gtggcaatta ttcttgtaag tattcaaata actgttgctt taaactatga
    15601 ttatctaaac tacataaatg gttcactgaa tcatcatcca agttagcaat taattgcatc
    15661 atttgttttg taaaagctgt gtctttttgt gaaattgcta agaaaaagac gagtttgaca
    15721 tcgtgttgtc gccatggaaa aacatctttt gtgcgaaaaa taagcacatg tgattgtaaa
    15781 actttttcag gatctccatg aggaatcgcc ataaaattac ctatgtatgt agaagatgat
    15841 ttctcacgct ctaaagctga ttcgatatat ccttctacaa tcgcatgatg tgcttgtaat
    15901 attttttgag cttcttcaaa aatttgcaca gtatgtcgtg gtttttgttc agtatttacg
    15961 acaaggaaat taacagtgtc catatgatga tgtgcttgaa ccggattttg cttttgcttc
    16021 acaacgtgta tgattttgtg acggtcatcc tcagaaaata atggtgcaac cttgatagtt
    16081 gaaaggtgct tagggagcat ttgtaaagtt tgctttggga tatcgtgagt tgtaattagt
    16141 aaatcaacat tgtcaaagtg atagtgtgtt atattttcta gtttaatcgt atttatcact
    16201 gacaactctt cggataagtt atttatttta gtttctaaaa aattcgacac acctagacca
    16261 taataacaag caatgactac atttaattgt gttttggtac gacgctcgat ggcagcttga
    16321 aaatgaattg ttaaaaaggc aatttcatct tcgctcatct ctatatcagt atcaattgct
    16381 aatttatcaa tcgcttcaaa aagtgtgttg aacacaaagg gataaagttt tttaatctct
    16441 ataactaaag gattgtttaa ataaatgttt tgagtgatac gtaaatatgc tttactaaaa
    16501 tgattatata aattttgttg taaaatcgaa tcttcattga aaggtacatg aatacgttgc
    16561 tgcatcaatt cgattaagcg atcaatataa ctttgtataa atatacgttc tatgccaata
    16621 tcgagtttat taaaatgata agcaataaag aatgaaaaca tattgattac tttttcattc
    16681 aagtcataac ctaatctttc gttgatttgc ttaatgcaag attgagatat caattttgca
    16741 tcgtggttta acgtgtctag ctcacttaaa gattgcttac gtttaatgat taaaatgaga
    16801 tgaattaaaa gctgttgtat ttgaatatca gttgtttcaa tactatgttg ttgaagtgtc
    16861 tcttgtataa tatgcgaaat catcctttgg tgtgaatcag gtaattcatt taaaattagg
    16921 tcttcaacat gtacatgccc tgatgataat tgatttaaat ggatgatggc attagtgata
    16981 tcattatctg ttccatcgac cacaatacct tgttttttct taattgttaa agcgatatta
    17041 aatttttgac accattgttg aattcgaacg aaatagtcgt tgagcacagt tttagataat
    17101 agataatctt gctctagttg ttgcaatgtc tgtgatttat tgtgcattag cagttgatac
    17161 gccatagata ataatagttt ttctttatat gattgaatat gcgtgtaacg ttcagttatc
    17221 gtttctaagg tatactgcga tgtatttaat tgatatcctt tagatttaat acttacgatg
    17281 atatcatcca taaaattgct attgataaca tggatatcat tgcgaactgt gcggtttgaa
    17341 acattgacat gttcagcaat ttcattagaa ctaatgtgct ttgatgggtt tttaataaaa
    17401 aactggagta gttttaagtg tctatcaagc atttaacatg tacctccttt ctaaattttt
    17461 cgtgtaagcg tttttaaatg tgtattaata ttattaaaca tgagagctta tacatacgtc
    17521 aatgacatta aagcgaactt ttatatgaat ttaacaaagt gagaattatg caaataaaga
    17581 acagcagtaa gatatttcaa atagaaaaat atctcattgc tgtttttctg aatttatgta
    17641 cctgatactt gtggacgtat caggtaaatg attaattttt agatgattgc ggttgtgatg
    17701 gtgatgcctg cttagaagac tcgtttgtat tagcattatt agatttgttt ggggccggtg
    17761 tattctgttg tttatgattt tggttattcg ttgaaggttg actgctaccg ccctgatttg
    17821 aatttgattg atttgaattt gattgattcg gattagatgg ttgtgtattc gctggtgctg
    17881 ttgaaccgtt attttggaca gttgatggcg tagatgattg attgccatta gtaacaccgt
    17941 tgttgctgtt ttgattttca tttgtgttgt ttgtattctc gtctttttga tgatttactt
    18001 cagtatcttt cttttgatgg gtagctttat tatcttgctc attattatga tttgatgatg
    18061 tagaatgatt tattgcagca taaatagcga atccaagtgc accaattaac aagatgataa
    18121 caacagtaat aagaaatttt ttcaaatgta acgcctccgt ttataataaa aatagtcata
    18181 cattgcatgg catcatgctt tcaatgtcga taaaatgatg ttaactataa aacttgaaat
    18241 gcggacgata ttaatatttg tgtcagtttt ttatgtactt caaataagta taacaaaact
    18301 gttatatatt tataaatata atcaatagta ttttattgta caagcaagtt ataataatag
    18361 taataagatt gaaaaatttg tatggaaaag agagataaat atgaatcaaa caactaaact
    18421 ttgtgcagca acgacattag cattaggtac tttaattggc gcaacagttg tagggactgc
    18481 atcaccaata catcaagaag cacaggcggc aacaacacct tactatactt atcatggata
    18541 tatcggtcat aacgctaatt ttataatgga taagcatttt attaacgcta ttaaatatga
    18601 caacgtgaca tttaatggta ttaaattagc gaagacaaat gcgacaaaaa aagtagagaa
    18661 atacgatcaa acttttaaag gtgtaactgc aaaaggaaat gaagcgaatc aattacaatt
    18721 tatcgttaaa aataatatat cactaaaaga tattcaaaaa gcttatggta aagatttgaa
    18781 aaaagagaat aataaaatga aagaatctga tagcggtatt ttttattatc aacattctaa
    18841 aaagtcatta ggcatttggt ttgttgttga tcataataga gtggttgaag ttacagtggg
    18901 acatacgcca tacaaaacga gtaaataata aagtaaatat aggggctagt atgtgaggaa
    18961 tgatgtcaca tactagtctt ttttgattat atgatggtag ttttcgtgtc tccatgatat
    19021 tgaaatatat agatttaatc atgcgcagtt aagtagtcag catatatatt tcacatgttg
    19081 attaagtatg tgttgtacta attttttgat tgttaataat ttcacaaaaa taaagccttg
    19141 ataaatttta aaagataatt aagtgcaatt ttcaaaattt tatttagtca cagataacat
    19201 ttttaaaaaa gaaaagaatc aataaataaa atcaacgaac aaaaagtata gaaataaata
    19261 gaaataatcg tttacttttc tgaaaaatta aattaatatt ttatttataa gctgttttta
    19321 aaatttcagg aggaatgaaa tgtgaggaaa ttttcaagat atgcatttac aagtatggca
    19381 acagtaacgt tgctgagctc tttgacacct gcagcactag cgagtgatac gaatcacaaa
    19441 ccagcaactt cagatattaa ttttgaaatc acgcaaaaga gtgatgcagt taaagcatta
    19501 aaagagttac ctaaatctga aaatgtgaaa aatcattatc aagattactc tgttacagat
    19561 gtaaaaacag ataagaaagg attcacgcat tacacgttac aaccgagtgt ggatggtgtg
    19621 catgcgcctg acaaagaagt gaaagtgcat gcggacaaat cgggtaaagt cgttttaatc
    19681 aacggtgata ctgatgcgaa gaaagtaaag ccgacaaata aagtgacatt aagcaaggat
    19741 gaagcggctg acaaagcatt taacgcagtt aagattgata aaaataaagc taaaaacctc
    19801 caagatgacg ttatcaaaga aaataaagtc gaaatcgatg gtgacagtaa taaatacatt
    19861 tacaatattg aattaattac agtaacacca gaaatttcac attggaaagt taaaattgat
    19921 gcagacacag gagcagttgt tgaaaaaacg aacttagtta aagaagcagc agcaactggc
    19981 acaggtaaag gtgtgcttgg agatacaaaa gatatcaata tcaatagtat tgatggtgga
    20041 tttagtttag aggatttgac gcatcaaggt aaattatcag catacaattt taacgatcaa
    20101 acaggtcaag cgacattaat tactaatgaa gatgaaaact tcgtcaaaga tgatcaacgt
    20161 gctggtgtag atgcgaatta ttatgctaaa caaacatatg attactacaa aaatacattt
    20221 ggtcgtgagt cttacgataa ccatggtagt ccaatagtct cattaacaca tgtaaatcat
    20281 tatggtggac aagataacag aaataacgct gcatggattg gagacaaaat gatttatggt
    20341 gatggcgatg gccgcacgtt tacaaattta tcaggtgcaa atgacgtagt agcacatgag
    20401 ttaacacatg gcgtgacaca agaaacggcg aatttagagt ataaagatca atctggtgcg
    20461 ttaaatgaaa gcttttcaga tgtttttgga tactttgtag atgatgagga tttcttgatg
    20521 ggtgaagatg tttacacacc aggaaaagag ggagatgctt tacgaagcat gtcaaaccca
    20581 gaacaatttg gtcaaccatc tcatatgaaa gactatgtat acactgaaaa agataacggt
    20641 ggtgtgcata cgaattctgg cattccaaat aaagcagctt ataacgtaat tcaagcaata
    20701 gggaaatcta aatcagaaca aatttactac cgagcattaa cggaatactt aacaagtaat
    20761 tcaaacttca aagattgtaa agatgcatta taccaagcgg ctaaagattt atatgacgag
    20821 caaacagctg aacaagtata tgaagcatgg aacgaagttg gcgtcgagta aaaatatata
    20881 aacaagaaga agtaatgtta aacacttata aataattaaa ttttaaatac agcttatata
    20941 agggttatac ctacattgat aattgacttt gtaggtgtac cctctttttg taattagatg
    21001 agaggagtat gagggttgtc tgccgaaaga ctactcgcca gtctaaaatc attactagta
    21061 gtaaatatgt gataattaaa tgctgactta gaatacaaaa ttcattttaa aagttgtcac
    21121 aaaaaattta catgtatttt tattatcttt tgcaaaacaa agtgttaaat tataaatgaa
    21181 acatgcatga atttattttt taatacaaga aacgtaacta ccaaaggagt ttacaatatg
    21241 aagaaaagta aacgattaga aattgtttct acaatagtta aaaagcataa gatttataaa
    21301 aaagaacaaa tcatttcata tattgaagaa tattttggtg taagatatag tgcaacaaca
    21361 attgctaaag acttgaaaga acttaatata tatcgtgtcc ctatcgattg tgagacatgg
    21421 atttataaag ctattaataa tcaaacagaa caagagatga gagaaaagtt taaacattat
    21481 tgtgaacatg aagtacttag ctcgattatc aatggcgcat atattatagt caaaacctca
    21541 ccaggtttcg ctcaaggcat taactacttt atcgatcaat taaatattga agaaatatta
    21601 ggtacggtga gtggaaatga cactacatta atcttaactg catcaaatga tatggcagaa
    21661 tacgtatatg caaaattatt taaatagaca tgtatcaaat gaataataaa aatttgtttt
    21721 gtatcacgtg tacacaagtt agttactaca tattaacttg tgtacgcgtt tttttatgga
    21781 aagaaagaat tcatagtcat tcatttgact gtataaaaaa ctttatacaa catgtttttg
    21841 tgggtgtttt tgaataaaaa atgtatattt taacccaaaa tacctttatt tatgtataaa
    21901 aatccattat tatgtattgt ataacaaaaa gatatgaaat tttcgacttt ctttatgtga
    21961 atataatcac atgtaagcgt ttgaagattg tctatactct aaatgaattc aaagataaaa
    22021 ggaggaaata gacatgacag atggtccaat taaagtaaat agcgaaattg gagctttaaa
    22081 aactgtgtta cttaagcgtc ctggaaaaga attagaaaat ttagtgcctg attatttaga
    22141 tggattacta tttgatgata ttccatattt agaagtagct caaaaagagc atgaccattt
    22201 tgcacaggtg ctaagagaag agggtgttga agtactttac cttgagaagt tagcagctga
    22261 aagtattgaa aatcctcaag taagaagtga atttattgat gatgtattag cagagtctaa
    22321 aaaaacaata ttaggtcatg aagaagaaat taaggcatta tttgcgacac tttctaatca
    22381 agaacttgta gataaaataa tgtcaggagt acgtaaggaa gaaattaatc cgaaatgtac
    22441 acatctagta gagtatatgg atgataagta tccattctat ttagatccaa tgccaaacct
    22501 ttattttact agagatccac aagcctcaat aggacacggt ataacaatca atcggatgtt
    22561 ctggagagca cgacgacgag aatcaatatt tattcaatat attgtaaagc atcatcctag
    22621 atttaaagat gcgaatattc caatctggtt agatcgagat tgcccattca atattgaagg
    22681 cggcgatgaa cttgttttat ctaaagaagt cttggctata ggcgtttcag aacgtacatc
    22741 tgcacaagct attgaaaagt tagcgcgacg tatttttgaa aatccgcagg cgacgtttaa
    22801 aaaagtagta gcaattgaaa ttccaactag tagaacattt atgcacttag atacagtatt
    22861 tacaatgata gattatgaca aatttacaat acattcagcc attttaaagg cagaaggcaa
    22921 tatgaatata tttattattg aatatgatga cgtaaataaa gatatttcca tcaaacaatc
    22981 tagtcattta aaagatactt tagaagacgt tctaggtata gatgatatcc aattcattcc
    23041 aacaggaaat ggtgatgtca ttgatggtgc tagagagcaa tggaatgatg gctcaaatac
    23101 attatgtata agaccaggtg ttgtagtgac ttacgataga aactatgtat cgaatgattt
    23161 attgagacaa aaaggtataa aagtcattga aatatctggt agcgagttgg tacgtggacg
    23221 tgggggtcct agatgtatga gtcaaccact attcagagaa gacatttaac gtaataaatt
    23281 atagaagcga gggtgaaaat aatgacagaa attcaaaaac cgtatgattt aaaaggcaga
    23341 tcattattaa aagaaagtga ttttaccaaa gcagaattcg aaggacttat tgattttgca
    23401 attacattaa aagagtataa gaaaaatggt atcaagcatc actacttatc tggaaaaaat
    23461 attgcactac tattcgaaaa gaattcgacg agaacgcgtg cagcgtttac agttgcgtct
    23521 attgatttag gtgcgcatcc agaattttta gggaaaaatg atattcaatt aggcaaaaaa
    23581 gaatctgtag aggatactgc gaaagtatta ggtagaatgt tcgatggtat tgaattccgt
    23641 ggtttttcac aacaagctgt tgaagattta gcgaagttct ctggtgtacc ggtgtggaat
    23701 ggattaacag acgattggca tcctacacaa atgttagctg attttatgac aataaaagag
    23761 aattttggat atctggaagg aataaactta acttacgttg gagatggacg taataatatt
    23821 gcgcattcat taatggtagc aggtgctatg ttaggtgtta atgtaagaat ttgtacacct
    23881 aaatcattaa atccaaaaga tgcatatgtt gatattgcaa aagaaaaagc gagtcaatat
    23941 ggtggttcag tcatgattac ggataatatt gcagaagcag ttgaaaatac agatgctata
    24001 tatacagatg tttgggtatc gatgggtgaa gaaagtgaat ttgaacaacg tattaattta
    24061 ttaaaagact atcaagtgaa tcaacagttg tttgatttaa caggtaaaga ttcaacgata
    24121 ttcttacatt gtttaccagc attccatgat acaaatacac tttatggaca agaaatttat
    24181 gaaaaatatg gattagctga aatggaagtt acagaccaaa tctttagaag tgaacattca
    24241 aaagtgtttg atcaagctga aaatagaatg catacaatta aggcagtaat ggcagcaaca
    24301 ttggggagtt aatcactaaa tggaacgata tgaatatgat gtgtctgatg atataagtat
    24361 caagtacaga cacctcatat tggtattaaa ggagaaatga atatgaacga atcaggagat
    24421 aacaaactca gtaaatcttc tttaattgga ctagttatag gatccatgat tggtggcggt
    24481 gcgttcaata taatgtctga tatgggtggt aaagccggtg gattagccat tattattggt
    24541 tggattatta cagctatagg aatgatttca ttagcgttcg tatttcaaaa tttaaccaat
    24601 gaacggccgg agctagacgg tggtatttat agttatgctc aagcaggatt tggcgatttt
    24661 gtaggcttta tcagtgcttg gggatattgg ttctcagcat ttttaggcaa tgttgcctat
    24721 gcaacactat tgatgtcagc agttggtaac tttttcccga tttttaaagg aggcaacaca
    24781 ttaccaagta ttattgtcgc ctcgttacta ctctggggtg tgcatttcct aattttaaaa
    24841 ggtgttgaaa cagcagcctt tatcaatagt attgttactg ttgcaaagtt aataccgatt
    24901 ttacttgtaa tcatatgcat gataattgca ttcaattttg acacttttaa aacaggcttt
    24961 ttcggtatga cgtcagaggg tgtattgcca tttagttggg cgagcacaat gagccaagtt
    25021 aaaagtacga tgctagtgac agtttgggtg tttatcggta tcgaaggtgc agtaattttt
    25081 tctagcagag ctaaaaataa aaaagatgta ggtagtgcca cggttatagg acttatatca
    25141 gttttaatta tctatttctt attaactgta ttagctcaag gcgtgatttt gcaaaatcat
    25201 atttctcaat tagattcgcc aagtatggca caggtgcttg catctattgt aggtggttgg
    25261 ggatctacac ttgtaaatat tggtttaatt atttcggtac taggtgcatg gttaggatgg
    25321 acattgcttg ctggtgaatt acctttcatt gttgcaaaag atggattatt tccaaaatgg
    25381 tttgctaaag aaaataaaaa tggagcacct gtaaatgcac tgcttattac caatatatta
    25441 gtacaattat ttttaataag tatgctattt acacagagtg cgtatcaatt tgcattttca
    25501 ctagcatcaa gtgctatttt atacccttac atgttcagtg cattttacca agttaaatac
    25561 actttagagc atcgacagca agcaactact aaacaatgga cgattggtat catagcctca
    25621 atttatgcta tatggcttat atatgcagca ggtatcaatt acttattact gacgatgtta
    25681 ctttatattc cagctcttct tgtttattca atcgttcaaa agaataatca gacacgtttg
    25741 attaaatcag actatattct ttttatgatt attatcgtac ttgcagttat cgggttaatt
    25801 aagttattga tgggaacgat aaatgttttt taaaaggagc gacaaaaata tgaaagagaa
    25861 aattgtcatt gcattaggcg gtaatgcgat acagacaaaa gaagcaacag ctgaagcaca
    25921 acaaacagct attagacgtg cgatgcaaaa ccttaaacct ttatttgatt caccagcgcg
    25981 tattgtcatt tcacatggta atggtccaca aattggaggt ttattaatcc aacaagctaa
    26041 atcgaacagt gacacaacgc cggcaatgcc attggatact tgtggtgcaa tgtcacaagg
    26101 tatgataggc tattggttgg aaactgaaat caatcgcatt ttaactgaaa tgaatagtga
    26161 tagaactgta ggcacaatcg taacacgtgt ggaagtagat aaagatgatc cacgatttga
    26221 taacccaact aaaccaattg gtccttttta tacgaaagaa gaagttgaag aattacaaaa
    26281 agaacagcca ggctcagtct ttaaagaaga tgcaggacgt ggttatagaa aagtagttgc
    26341 gtcaccacta cctcaatcta tactagaaca ccagttaatt cgaactttag cagacggtaa
    26401 aaatattgtc attgcatgcg gtggtggcgg tattccagtt ataaaaaaag aaaataccta
    26461 tgaaggtgtt gaagcggtta tagataaaga ttttgctagt gagaaattag caacgctgat
    26521 tgaagcagat accttaatga ttcttacgaa tgtagaaaat gtatttatta actttaatga
    26581 acctaatcaa caacaaatcg atgatattga tgtagcaaca ctgaaaaaat acgcggcaca
    26641 aggtaagttt gcggaaggat cgatgttgcc aaaaatagaa gctgcgatac gatttgttga
    26701 aagtggggaa aacaaaaaag ttatcattac caatttagag caggcatacg aagctttgat
    26761 tggtaataaa ggtacacaca ttcacatgta gcactttatc acgcgacaaa acattaaata
    26821 tgtttctccg ttgattcaaa tgaaaaagtt gtctgctgac actttgcaag gtttgaagga
    26881 gtttaactta tgacagaaaa ctttattttg ggtagaaata ataaattaga acatgaacta
    26941 aaggcattag cagattacat taatatccca tatagtatat tacaaccata tcaaagtgaa
    27001 tgttttgtca gacattatac gaaaggccaa gttatttatt tttcgccaca agaaagtagc
    27061 aatatttact ttttaattga aggtaacatt attagagaac attacaatca aaatggagat
    27121 gtatatcgtt attttaataa agagcaagta ttatttccaa tcagtaactt atttcatccg
    27181 aaagaggtta acgaattgtg cacagcatta accgattgta cagttcttgg attgcctaga
    27241 gaattgatgg cctttttgtg caaagctaat gatgatatat ttttgacact ttttgcatta
    27301 ataaatgata atgagcagca acacatgaac tataacatgg cattaacaag taaatttgct
    27361 aaagatcgaa ttatcaaatt gttatgccat ctatgtcaga cagtaggata cgatcaagat
    27421 gaattttatg aaatcaaaca gtttttaact attcaactca tgagtgatat ggctgggatt
    27481 tcccgggaaa cagctggtca tattattcat gaacttaaag atgaaaaact tgttgttaaa
    27541 gatcataaaa attggttagt aagcaaacat ttattcaatg atgtatgtgt ttaatataca
    27601 atgtaaaatg aataagttga acatgaggtc taacgtacat ttatacgtta ggcctttttt
    27661 gttagcatga tgattaattt aatatgttag ttaaatttga ttgttgaaat tacagtaaaa
    27721 tttaaggtga tgaaaaattt agaacttcta agtttttgaa aagtaaaaca tttgtaatag
    27781 tgtaaaaata gtatattgat ttttgctagt taacagaaaa ttttaagtta tataaatagg
    27841 aagaaaacaa attttacgta atttttttcg aaaagcaatt gatataattc ttatttcatt
    27901 atacaattta gactaatcta gaaattgaaa tggagtaata tttttgaaaa aaagaattga
    27961 ttatttgtcg aataagcaga ataagtattc gattagacgt tttacagtag gtaccacatc
    28021 agtaatagta ggggcaacta tactatttgg gataggcaat catcaagcac aagcttcaga
    28081 acaatcgaac gatacaacgc aatcttcgaa aaataatgca agtgcagatt ccgaaaaaaa
    28141 caatacgata gaaacacctc aattaaatac aacggctaat gatacatctg atattagtgc
    28201 aaacacaaac agtgcgaatg tagatagcac agcaaaaaca atgtctacac aaacgagcaa
    28261 taccactaca acagagccag cttcaacaaa tgaaacacct caaccgacgg caattaaaga
    28321 tcaagcaact gctgcaaaaa tgcaagatca aactgttcct caagaagcaa attctcaagt
    28381 agataataaa acaacgaatg atgctaataa catagcaaca aacagtgagc ttaaaaatcc
    28441 tcaaacatta gatttaccac aatcatcacc acaaacgatt tccaatgcgc aaggaactag
    28501 taaaccaagt gttagaacga gagctgtacg tagtcttgca gttgctgaac ctgtagtaaa
    28561 tgctgctgat gctaaaggta caaatgtaaa tgataaagtt acggcaagtg atttcaagtt
    28621 agaaaagact gcatttgacc ctaaccaaag tggtaacaca tttatggcgg caaattttaa
    28681 agtgactgga caagtgaaat caggggatta ttttacagcg aagttaccag atagtgtaac
    28741 tggtaatgga gacgtggatt actctaattc aaataatacg atgccaattg cagacattaa
    28801 aagtacgaat ggcgatgttg tagctaaagc aacatatgat atcttgacta agacgtatac
    28861 atttgtcttt acagattatg taaatgataa agaaaatatt aacggacaat tttcattacc
    28921 tttatttaca gaccgagcaa aggcacctaa atcaggaaca tatgatgcaa atattaatat
    28981 tgcggatgaa atgtttgata ataaaattac ttataactat agttcgccaa ttgcaggaat
    29041 tgataagcca aatggcgcga acatttcttc tcaaattatt ggtgtagata cagcttcagg
    29101 tcaaaacaca tacaagcaaa cggtatttgt taaccctaag caacgagttt taggtaatac
    29161 gtgggtgtat attaaaggtt accaagataa aatcgaagaa agtagcggta aagtaagtgc
    29221 tacagataca aaactgagaa tttttgaagt gaatgataca tctaaattat cagatagcta
    29281 ctatgcagac ccaaatgact ctaaccttaa agaagtgact ggtgagttta aagataaaat
    29341 ttcatacaaa tacgataacg tagcaagtat taattttggt gatataaata aaacgtatgt
    29401 tgtattagtg gaaggtcact atgataatac tggtaaaaac ttgaaaacac aggttattca
    29461 agaaaatatt gacccagcga caggtaaaga ctacagtatt ttcggttgga ataatgagaa
    29521 tgttgtacgt tatggaggcg gaagtgctga tggtgattca gcagtaaatc cagttgatcc
    29581 aacaccggga ccaccagttg acccagagcc agaaccggag ccaacgccgg atccagagcc
    29641 aagtccagag ccagaaccgg aaccaacgcc agatccagag ccaagtccag aaccagatcc
    29701 ggattcggat tcagatagtg actcaggctc agacagcgac tcaggttcag atagctattc
    29761 ggattcagat agtgactcag actcagatag tgactcaaac tcagacagtg agtcagattc
    29821 agacagtgac tcggactcag atagtgactc agattcagat agcgattcag attcagacag
    29881 cgattcggac tcagacagtg agtcagattc agatagcgac tcggattcaa acagcgattc
    29941 ggactcagac agtgactcgg actcagatag tgactcggac tcagatagtg actcaaactc
    30001 agatagtgac tcagattcag acagcgattc ggattcagat agcgattcag attcagacag
    30061 cgattcagaa tcagacagct attcggattc agacagtgac tcggattcag atagcgactc
    30121 ggattcagac agcgattcgg actcagacag tgactccgat tcagatagcg attcagaatc
    30181 agacagtgag tcagattcag atagcgattc agaatcagac agtgactcag acccagacag
    30241 tgactccgat tcagacagcg attcagactc agacagcgat tcagactcag acagtgagtc
    30301 agattcagac agcgattcgg actcagatag cgactccgat tcaagagtta caccaccaaa
    30361 taatgaacaa aaagcaccat caaatcctaa aggtgaagta aaccattcta ataaggcatc
    30421 aaaacaacac caaactgatg ctttaccaga aacaggagat aagagcgaaa acacaaatgc
    30481 aactttattt gatgcaatgg tggcattatt aggatcatta ctattgttta gaaaacgcaa
    30541 gcaagatcat aaagaaaaag cgtaaatact tttttaggcc gaatacattt gtattcggtt
    30601 tttttgttga aaatgatttt aaagtgaatt gtttaagcgt aaaatgttga taaagtagaa
    30661 ttagaaaggg gtcatgacgt atggcttata tttcattaaa ctatcattca ccaacaattg
    30721 gtatgcatca aaatttgaca gtcattttac cggaagatca aagcttcttt aataacgata
    30781 caactgctaa accattaaaa actttaatgt tgttacatgg attatcaagt gatgaaacga
    30841 catatatgag atatacaagc atagaaaggt atgcgaatga acacaaatta gctgtgatta
    30901 tgcccaatgt ggatcatagc gcatatgcta acatggcata tggtcatagc tattatgatt
    30961 atattttgga agtatatgat tatgttcatc aaatatttcc actttccaaa aaacgtgatg
    31021 acaattttat agcaggtcac tctatgggag gatatggcac aattaaattt gcattaacac
    31081 aaggggataa atttgccaaa gctgtaccat tatctgctgt gtttgaagcg caaaatttaa
    31141 tggatctaga gtggaatgat ttttcaaaag aggccataat tggcaatctt tcaagtgtta
    31201 aaggaactga acatgatccg tattacttgc tagacaaagc tgtagctgaa gataaacaaa
    31261 ttccaaaatt gctcattatg tgtggtaaac aagacttttt atatcaagac aatttagatt
    31321 ttatcgatta tttatcacgc aaaaatgttc cttatcaatt tgaagatgga ccaggagatc
    31381 atgattatgc atattgggac caagcgatta agcgtgctat aacatggatg gtgaatgatt
    31441 aattatttcc tggaaaatat gtgcctacat taaatacaca gagtgagtga tacaaactat
    31501 ttacgcacga ctaacatttc tgagtgttta aattaatttt gtattaatat gattggtgca
    31561 atttgctgat acacaaaaat gtttctcgtg aaacttagat ttagcttata gttttatcat
    31621 catttgtatg acttacatta taaattttat tataatgagg ttaacgcttt gaaaggagtc
    31681 atcatcatgt cgaccaataa aaacgattat gagcatatgt tgttttattt tgcatataaa
    31741 acgtttatta ctaccgctga tgaaattata gagaagtatg gtatgagccg tcagcatcat
    31801 cgttttttgt tttttatcaa taaattacct ggtattacta ttaaatcatt actagaaata
    31861 ttagaaattt ctaaacaagg atcacatgca acacttcaaa aattaaaaga gcaaggtctc
    31921 attattgaaa aagttttaga gactgatcga cgtgtcaaaa aattatattc gacggataaa
    31981 ggcgatcaac tcattgctga attgaacaag gcgcaagatg aattattgca aaatatatat
    32041 caacaagtcg gttcggattg gtatgatgtg atggaagcat tggctaaagg gcgacctggc
    32101 tttgatttta ttaagcattt gaaagatgaa aaagaaagct agcatcagaa atgttgaaaa
    32161 tcttcgcatt cttaaattta aaaaatatgt caaaaagtgt ataataaaaa catataattt
    32221 aattgaactc agtttcaaca catcttagaa aggagtttga atgatgaaaa aattagcagt
    32281 tattttaaca ttagttggcg gtttatactt cgcatttaaa aaataccaag aacgtgttaa
    32341 ccaagcacct aacattgagt actaaattaa accataaaaa attcccgaac atcttgttat
    32401 agtgctcggg aattttttta tgctttactt gaatatatca aatattattt ttgcgctttc
    32461 tgtattttcg atattaccac taaatgattc tgatctaggt ccataagcgt aagtattaac
    32521 atcttcgcct gtatgtccat cggaagtcca tcctgtataa gatttatcat ttactggctt
    32581 ctgaattgcg tgttgtaagg cttttgtttg tttttctaca tctgcagatt tttcatcttt
    32641 ttctttttta agtagtcttt ttagcttttt attctctttt ttaacttttt tcatatcatc
    32701 ttgtgaaaat tcaaagccat aaccttcatt aataactttt tcagggtctt cacctttagc
    32761 cattttttct gtcatatatg atccagagtg tttcatcgat ttaatcggtt gaggattcca
    32821 ttcgtatcct ttatctttac caattgttag accaccagta gagtgatctg ctgttgcaac
    32881 gacaagtgta tctttatgtt tcttagcata ttgaatggcg tcatcgaatg ctttttcaaa
    32941 accttccatt tcagacatga ctcctgtaat atcgttagaa tgcgctgatt tatctataga
    33001 agcaccttcg accattaaaa agaaaccttt tttattacgc tcaagcttac taagtgcact
    33061 ttgttgcata tcagctaatg atggttcgtc tttagaagca tctattgcaa gtggcatatt
    33121 tttatctgca aacaaaccaa gcactttatc tttatcagat tttgataact ccttactgtt
    33181 cgtggcaagg tcgtaaccat cttttttgaa ttttttatct aaattgccat tacttttacc
    33241 gaaatattta gcgccaccgc ctaataaaac atcaacttta tgctttccgt tgattttatc
    33301 tttataaaat tgtttagcga tttcgttttt atcatctcta gaagtcacgt gtgcagcata
    33361 tgctgctggt gttgcatctg ttaattcagc tgttgaaaca agaccagtcg acttaccttt
    33421 ttcttttgca cgttcaagca ccgtctttac tttctgcttg ttactgtcaa caccgatggc
    33481 accattatat gtcttatgac cagaactaaa ggctgttccg ccagctgcag aatcagtaat
    33541 attctgtttt gggtcatttg aatatgtacg atttgtgcct tttaaatatg aatcaaaagc
    33601 agtaggagtc atttctttag catgaggatc atttttataa taacgataag ctgtgttaaa
    33661 tgatggaccc atgccatcgc caactaaaaa gataacattt tttggatttt tagtattacc
    33721 aaccgcgaaa ctttcatctt tagaactttt atcggattgc gcaattgcag gtgtgacaga
    33781 gctaaaaact gttgacacga taataaggtt agcaactgca aattttgtgg cttttttaac
    33841 tgataacata agacatcctc ctgagtatat gactatgtct tcagtgtaaa agaggaattt
    33901 tgagcaatta tgtagtttta gttaaaaata tgtaaacaga gtgatttaga ataacaaaaa
    33961 atgaatatat atgacaattt gttatagaaa gcgttagaat agaagcgtgt gaaaatatag
    34021 aattaaatat aatttgaggt ggaaaaatga tactagtaat gttatctcca ttattaatca
    34081 tattctttat agtgttgtct attttagaag agcgtaaacg tacgaagaaa aagcaactcg
    34141 agaaagaaaa agcaaataca ctaaatcaaa atacaaatga cacggaaagt tcaaatcaag
    34201 agccgtcatt gcagcaggct aaagaacaaa aagataacaa aggataattc aattgaagga
    34261 agaagattat agatgaaaat attaattgtt gaagatgatt ttgttatagc agaaagttta
    34321 gcatctgaac ttaaaaaatg gaattacggt gttattgtcg ttgaacaatt tgatgatata
    34381 ctgtctatct ttaaccaaca tcaacctcag cttgtattgc tagatattaa tttgccaacg
    34441 ttaaatggtt ttcattggtg tcaagaaatc cgaaaaacat ctaatgtgcc aattatattt
    34501 attagttccc gtattgataa tatggaccaa attatggcaa tacaaatggg gggagatgat
    34561 tttatcgaaa agccatttaa cttgtcatta acgattgcca aaattcaagc attattgaga
    34621 cgaacttatg acttgtcagt agctaatgat tcattgacgg ttaaaggttg tacattaata
    34681 ttagatgaag caaaagtcgt gtatcaagaa caaagcatac agctatcttt gactgaatta
    34741 caaatattaa agttattatt tcaaaatgaa gataaatatg taagtagaac tgctttaatt
    34801 gaaaaatgtt gggaatcaga aaacttcata gatgataaca cattagctgt taacatgacg
    34861 cgcctgcgta aaaaattaaa tactattggc gttaatgatt ttatcattac aaagaaaaat
    34921 gtcggatata aagtataggg tgaatgcaat gacctttctt aaaagtatta ctcaggaaat
    34981 agcaatagtc atagttattt ttgctttatt tggcttaatg ttttacctgt atcatttgcc
    35041 attagaagca tatttactag cacttggcgt tattttatta ttattactca tattcatagg
    35101 tattaaatat ttaagttttg taaaaactat aagccaacaa caacaaattg aaaatttaga
    35161 aaatgcgttg tatcagctta aaaatgaaca aattgaatat aaaaatgatg tagagagcta
    35221 ctttttaaca tgggtacatc aaatgaaaac acccattact gcagcacaac tgttacttga
    35281 aagagatgag cctaatgttg tgaatcgtgt tcgtcaagag gttattcaaa ttgataacta
    35341 tacaagttta gcacttagtt atttaaagtt attaaatgaa acttctgata tttctgtcac
    35401 taaaatttcg attaacaata tcattcgccc aattattatg aaatattcaa tacagtttat
    35461 tgatcaaaaa acaaaaatcc attatgaacc ttgtcatcac gaagtattaa ctgacgttag
    35521 atggacctct ttaatgatag aacaattaat aaataatgca cttaagtatg cgagaggtaa
    35581 agatatatgg attgaatttg atgagcaatt caatcaatta tacgtaaaag ataatggtat
    35641 cggtattagt gaagcggact tgcctaaaat atttgataag ggctattcag gttataatgg
    35701 ccagcggcaa agtaactcaa gtgggattgg tttatttatc gtaaaacaaa tttcaactca
    35761 cacaaaccat cctgtttcag tcgtatctaa acaaaatgag ggtacaacat ttacgattca
    35821 atttccagat gaataaaaac tttcaatatt gtaagcatac tagtaacatt ttttcactaa
    35881 tttaaatgtt attagtattt ttttgtttta atatagaact aacaaagaaa tgaggtgcat
    35941 gccatgttgc tagaagtgaa acatgtaaaa aaggtttatg gtaaaggttt gaatgctacg
    36001 acagcactta atcaaatgaa tttatcagtt ggagctggtg aatttgttgc aattatgggt
    36061 gagtctgggt cagggaagtc tacactacta aatttaattg cttcttttga tggattaact
    36121 gaaggtgaca ttattgtgga tggcgcacat ttaaataata tgaaaaataa aagtaaagca
    36181 ttgtatcgtc aacaaatggt aggttttgtt tttcaagatt ttaatctttt accaacaatg
    36241 acgaataaag aaaatataat gatgccatta attttagctg gtgctaaacg aaaagatata
    36301 gaacaaaggg tacatcagtt gacagtacaa ttacatttag agggattctt aaacaaatat
    36361 ccttctgaaa tctcaggggg tcagaagcaa cgtattgcca ttgcacgtgc attagttact
    36421 aaaccgacga ttttactagc cgatgaacct acaggtgcac tggattcaaa aacatcaaaa
    36481 gcattgatga agttatttca agagattcat caattggaac agacaatttt aatggtaact
    36541 cattcaaata tcgatgcgtc ttatgcagag cgagtcattt ttattaaaga tgggcgtcta
    36601 tatcatgaaa tatatcgtgg tgaagaaagt caattagttt ttcaacagcg aataacagat
    36661 agcttagcac ttgtgaatgg aggaagtgtc aatatatgaa gttaagattg ttatggcaca
    36721 tagtacgacg tcaatttatt acgcagcgac ttgtaatcat tccattcatt ttagcggtaa
    36781 gtgtactatt catgattgaa tatacgcttg tatcaattgg gttaaatagc tacataaaac
    36841 agaagaatga cttcctagta ccatttatta tcatagctaa tttttttatg gcgcttttaa
    36901 cttttatttt tattttctat gcaaatcact ttatgatgtc acaaagacga aaagagttta
    36961 gcatttttat gacattgggc atgaccaaga aaagtatgcg tttaattgta gtgatggaaa
    37021 ctatcttaca atttatgata atttcagtca ttagtattgc cggcggatac ttacttggtg
    37081 cgatattttt cttgtttata cagaaaataa tgggcagtga agttgctacg ttaaggtatt
    37141 atccatttga tactgtagcg atgtttatta ctttgattat cattgctgta ttaatgggca
    37201 tgctacttat attcaacttg ttcagtatta attttcaacg accgataact tatcaacatc
    37261 gttccgattc cagtgtcata tcacgatggt tgcgttacgt tttaattgtt ataggaagcg
    37321 cagcactata tttaggctat tttattgcat tacaacaaga tacgacgttt ggtgcttttt
    37381 ttaaaatatg gattgtcata ggattagtta ttatcggtac ttatgcattt tttgtaggta
    37441 taagtgaaat aattattagt atattgcagc aggtatcaaa agtttactat catccacggt
    37501 atttttttgt ggtagttggg atgcgtgtac gtcttaaaat gaatgcagtc agtcttgcaa
    37561 caatcacttt gctgtgtaca tttttgattg taacgctcac aatgacacta acaacctatc
    37621 gcgatatgaa tcataccatt atgaagttga ttaccaatga ttatgatttg tcatttaacg
    37681 acaattctaa gtcacaaata gaacgtcaac aaacgattga gcatattaaa cgtgatatac
    37741 agggttcggt taatgcaaaa gacttcaaag tatatgaaac gacattattt agagcttctt
    37801 tagaaaagaa tcgtgcttat tacaagttaa aacaggctag tgatgatatt ccaatagact
    37861 tcattggtaa tgaaggctcg atattttcta agcaatcagt aatgatcaca gcacttactg
    37921 tgcaagatta taatcgatat caccaaaaca aattacactt agacaatcaa agtataggta
    37981 tgattacgaa tgtacctatt tttaaaaagc agtctaaggt cggtcttaat aatgaagtct
    38041 ttaaagtgaa acaacttgat gcacatgcgt taaatttgat gatgattcaa gatagtatgg
    38101 ttttaattgt taaagataac aatcaattac aaaaagtaaa aggattttat gcacatgaac
    38161 aaaagaagtc gacaatatcg ataaacttta atgtatctgg aaagacgaaa ttaacaacat
    38221 cacaaattca aaagctttcg aaaaagtacg atgcatcaat aaattctaaa tcagaaatgc
    38281 taatggtttg ggatcgtctg acaggtggac taatatttgt aggtggcgtg gtatcaattg
    38341 tacttgtcat tggaatattt ctaatgatgt actataagca agtttcagaa gcctatgcaa
    38401 atcagcacaa ttatgacatt atgaaaaaac ttggtttaga taatgggaga atatcaaaga
    38461 taacacgaaa tcaaatgact ttcttgttcg caataccaat aacagtagca ctgattcata
    38521 cattaatttc tagcaacatt gtttatacgt tactaaatat gctagggatt aataaccatc
    38581 atatattttt aacatgttac gtactagctg tcattataat ttcgtttcta tatatggcga
    38641 tgtacaaaat aacttcctac atttatgcta aagttattca tcaacaacgc aattaaccat
    38701 gtttcagtga aatcacaatc aatcatgtgt tttcactgaa atatattata tgaaaaagaa
    38761 aacctaaaaa tactacaaat aacaataaca attttcaacc gataagataa aatgtcttat
    38821 cggtttttta cttgtatgac aagttatatt tttgaaatat caaaaatata tagcttgttt
    38881 atataaaatg attcaaaatg ttttataaca acatatttta tgcaacacat aaaatgggta
    38941 actaacatca cgaaatcatt gtatttgtag ttacactgat gaagcgatgt cacaaagaaa
    39001 tgtcgttatc aaacgaagcc cgaaatacat actgatactc gaagtaatgt aacgataaat
    39061 aacaatgata acgattgatt actacgcaaa aatatatgta aattgacg
//
LOCUS       DAMZPP010000004        38971 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMZPP010000004 DAMZPP010000000
VERSION     DAMZPP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 38971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38971
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.004"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(140..709)
                     /locus_tag="POH73_000149"
     CDS             complement(140..709)
                     /locus_tag="POH73_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDI6886138.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
     gene            919..1137
                     /locus_tag="POH73_000150"
     CDS             919..1137
                     /locus_tag="POH73_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HDI6886139.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYMAEYKNLEAFLGE"
     gene            complement(1218..2204)
                     /locus_tag="POH73_000151"
     CDS             complement(1218..2204)
                     /locus_tag="POH73_000151"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDI6886140.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            2403..2579
                     /locus_tag="POH73_000152"
     CDS             2403..2579
                     /locus_tag="POH73_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HDI6886141.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            2594..3196
                     /locus_tag="POH73_000153"
     CDS             2594..3196
                     /locus_tag="POH73_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDI6886142.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            4113..4214
                     /locus_tag="POH73_000154"
     CDS             4113..4214
                     /locus_tag="POH73_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886143.1"
                     /translation="MKFAANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     gene            4224..4496
                     /locus_tag="POH73_000155"
     CDS             4224..4496
                     /locus_tag="POH73_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDI6886144.1"
                     /translation="MFGTILYLSLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HAHKGHGATAIGKYRSFSGYTRASVKAKASATKHNLRVNRAYYNIY"
     gene            4540..6504
                     /locus_tag="POH73_000156"
     CDS             4540..6504
                     /locus_tag="POH73_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDI6886145.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRINQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKATTITPFNAKY
                     NIHYLSDDELLKKDIKGSYFVKDKNFDVTKFINFLKEYGVTAESYKIDYMMIAVGVIK
                     QMNIVVPLSSLLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKIIFENFSKKCMYWVTL
                     IITQILLTTSVLWILNYTGNLDLFILRLVLLSCLFILTISVINLWTFLMLLNLNIANM
                     IKGKQHFKTIRFINTVCKSVLLVLIASVMIENTSVIKDLNKIKETEKYWNVLDDYYTI
                     EFAPYHETKQSLIDNMVRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVN
                     NQFWSIYHKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKES
                     KLSIQFINKNDYRIFTFDARDSRHLSFIEAPIIVNVQASDLSKDFYYAMISQGGYLFK
                     NYDALVKNIEKYHLDGEISGITNYKDSVMEMYHENNLKLTVLNFSQIIIAIILIIIIL
                     FDVKYYFEQHRKLLVIKKLYGYSTLRANYQYLLINNIVVVFIGILTNVILHSHYIMMI
                     FATIIVVQILLQICSLYYHGRRFNEVIKEF"
     gene            6507..6827
                     /locus_tag="POH73_000157"
     CDS             6507..6827
                     /locus_tag="POH73_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDI6886146.1"
                     /translation="MKYIIAILLGLIVSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKDDTPKE
                     VRQE"
     gene            6824..7465
                     /locus_tag="POH73_000158"
     CDS             6824..7465
                     /locus_tag="POH73_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6886147.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIVNLTVNENLDIGLAYK
                     KISKKEKEQLKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFDLIDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
     gene            complement(7553..7843)
                     /locus_tag="POH73_000159"
     CDS             complement(7553..7843)
                     /locus_tag="POH73_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886148.1"
                     /translation="MFFACHFKMKIGGVILTRTYNIIGILSCLISFIIMALPMIWYVA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLLVLNIIILFFFSLPLLLS"
     gene            8186..8473
                     /locus_tag="POH73_000160"
     CDS             8186..8473
                     /locus_tag="POH73_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886149.1"
                     /translation="MEKGIFNYDNANVLKLDTNQLNENIKVIDDIFKNYEQIEPTIEI
                     ENGNTKLKLNGYFIASIISPLNLNKLNNLYVEEEFYHTYNELIVKYTEVKE"
     gene            8479..9960
                     /locus_tag="POH73_000161"
     CDS             8479..9960
                     /locus_tag="POH73_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI6886150.1"
                     /translation="MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYK
                     GNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEI
                     EESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVD
                     NKVCYQVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDS
                     KDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKI
                     NGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRID
                     LLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLED
                     FKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMA
                     DKILKLVNNDVLAEEFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(10049..10405)
                     /locus_tag="POH73_000162"
     CDS             complement(10049..10405)
                     /locus_tag="POH73_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDI6886151.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
     gene            10896..11855
                     /locus_tag="POH73_000163"
     CDS             10896..11855
                     /locus_tag="POH73_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6886152.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRIVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHNKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTAHVGERKGGPYIYLNNEELANINPKVMILATNGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLQSRGIMASESMAEDLEKIAEKAK"
     gene            complement(11902..12033)
                     /locus_tag="POH73_000164"
     CDS             complement(11902..12033)
                     /locus_tag="POH73_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886153.1"
                     /translation="MRYVTMRQFIKRIVKTILVGYVIKFIRNKLSGKSSHPTDNKHK"
     gene            complement(12097..12312)
                     /locus_tag="POH73_000165"
     CDS             complement(12097..12312)
                     /locus_tag="POH73_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HDI6886154.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIIVIFTKKADKDGYIIFPKN"
     gene            12477..13028
                     /locus_tag="POH73_000166"
     CDS             12477..13028
                     /locus_tag="POH73_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6886155.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDIVLDAIEKS
                     CVDATYRTFYQHVLVYEIENKVAGCIISYSGENELKYEKAWEQLDLPEEIKQYGTPLP
                     VKEAKDDEYYIETIATFEAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(13080..14018)
                     /locus_tag="POH73_000167"
     CDS             complement(13080..14018)
                     /locus_tag="POH73_000167"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDI6886156.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISTLFAGI"
     gene            14200..15561
                     /locus_tag="POH73_000168"
     CDS             14200..15561
                     /locus_tag="POH73_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDI6886157.1"
                     /translation="MATGVLEDDIVKEIYDSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRECFTVEALNDL
                     TDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            15548..17221
                     /gene="menD"
                     /locus_tag="POH73_000169"
     CDS             15548..17221
                     /gene="menD"
                     /locus_tag="POH73_000169"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDI6886158.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLKVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQRIEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRVTLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNNVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            17208..18011
                     /gene="menH"
                     /locus_tag="POH73_000170"
     CDS             17208..18011
                     /gene="menH"
                     /locus_tag="POH73_000170"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDI6886159.1"
                     /translation="MTHYNYYEANVETNQVLVFLHGFLSDSRTYHNHIDKFTDNYHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDEARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPQKMAKALRDYGTGQMPNLWPRLIEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            18004..18825
                     /gene="menB"
                     /locus_tag="POH73_000171"
     CDS             18004..18825
                     /gene="menB"
                     /locus_tag="POH73_000171"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDI6886160.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(19061..19390)
                     /gene="sspC"
                     /locus_tag="POH73_000172"
     CDS             complement(19061..19390)
                     /gene="sspC"
                     /locus_tag="POH73_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HDI6886161.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSFDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(19428..20609)
                     /gene="sspB"
                     /locus_tag="POH73_000173"
     CDS             complement(19428..20609)
                     /gene="sspB"
                     /locus_tag="POH73_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HDI6886162.1"
                     /translation="MNSSCKTRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLANNIKEKESAKTVSPQLKQELKTTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYNQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVANAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(20691..21764)
                     /gene="sspA"
                     /locus_tag="POH73_000174"
     CDS             complement(20691..21764)
                     /gene="sspA"
                     /locus_tag="POH73_000174"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HDI6886163.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQT
                     DKQQTPKIQKGGNLKPLEQRERANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNQFNGAVFINENVRNFLKQ
                     NIEDINFANDDHPNNPDNPDNPNNPDNPNNPDNPNNPDNPDNPNNPDNPNNPDNPNNP
                     DQPNNPNNPDNGDNNNSDNPDAA"
     gene            complement(22292..23446)
                     /locus_tag="POH73_000175"
     CDS             complement(22292..23446)
                     /locus_tag="POH73_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6886164.1"
                     /translation="MKLSLNSNSKFLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPIFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPNIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
     gene            complement(23641..24651)
                     /locus_tag="POH73_000176"
     CDS             complement(23641..24651)
                     /locus_tag="POH73_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDI6886165.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIIYVIHPINADWLLGSSP
                     YTSLENEGQSVFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
     gene            24804..25223
                     /locus_tag="POH73_000177"
     CDS             24804..25223
                     /locus_tag="POH73_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6886166.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDITSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            25250..25360
                     /locus_tag="POH73_000178"
     CDS             25250..25360
                     /locus_tag="POH73_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886167.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(25431..29204)
                     /locus_tag="POH73_000179"
     CDS             complement(25431..29204)
                     /locus_tag="POH73_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDI6886168.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKAANKTGNAQVNQKVDTTQVNG
                     DTRATQSTTSNNAKPVTKSTNTTAPKTNNNVTSAGYSLVDDEDDNSENQINPELIKSA
                     AKPAALETQYKAAAPKATPVAPKAKTEATPKVTTFSASAQPRSAAAAPKTSLPKYKPQ
                     VNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTINGEI
                     SYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASFAR
                     SMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSHNY
                     SYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPSTPSKPSTPSTGKLTVAANNG
                     VAQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWV
                     KEGDVVYNTAKSPVNVNQAYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQ
                     IYKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQIN
                     AKNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDV
                     IYNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSI
                     YLYGTVNGKSGWISKAYLAVPAAPKKAVAQPKTAVKAYAVTKPQTTQTVSKIAQVKPN
                     NTGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLN
                     VQNLGKEVKTTQKYTVNRSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDV
                     YLYGTINNRTGWVNSKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKY
                     SLKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQIGMTLNQV
                     AQIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISI
                     DKINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNN
                     KVVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAG
                     QNTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(29432..29866)
                     /locus_tag="POH73_000180"
     CDS             complement(29432..29866)
                     /locus_tag="POH73_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6886169.1"
                     /translation="MFSKVIDPEMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVIKSHRGQGMGKMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(30022..30492)
                     /locus_tag="POH73_000181"
     CDS             complement(30022..30492)
                     /locus_tag="POH73_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HDI6886170.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIENPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(30540..31766)
                     /locus_tag="POH73_000182"
     CDS             complement(30540..31766)
                     /locus_tag="POH73_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HDI6886171.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDSSLLRKEQNDTTDKDKSSNNTDDSNNSNNNNSVNNQQQPATDQNLNQNQGG
                     TQQTPQASNNQNGVVN"
     gene            32207..33400
                     /gene="fmtA"
                     /locus_tag="POH73_000183"
     CDS             32207..33400
                     /gene="fmtA"
                     /locus_tag="POH73_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HDI6886172.1"
                     /translation="MKFNKEKLVIHACVLLFIIISIGLVFHRLQTKTNSIEPIHKETK
                     LSDNAKYLVDRNKGKGEPSKLKEVYNSKDPKYKKIDRYLQNSLFNGSVAVYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYIPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVRAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHSAFYDEKSFRKYFAKGYSYNSTGLSFLKP
                     NVLEQYYGAGNIYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKQYPDEYRYGFY
                     VKPTLNRLNGGLFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
     gene            complement(33743..34021)
                     /gene="qoxD"
                     /locus_tag="POH73_000184"
     CDS             complement(33743..34021)
                     /gene="qoxD"
                     /locus_tag="POH73_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HDI6886173.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFIQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(34030..34635)
                     /gene="qoxC"
                     /locus_tag="POH73_000185"
     CDS             complement(34030..34635)
                     /gene="qoxC"
                     /locus_tag="POH73_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HDI6886174.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(34625..36613)
                     /gene="qoxB"
                     /locus_tag="POH73_000186"
     CDS             complement(34625..36613)
                     /gene="qoxB"
                     /locus_tag="POH73_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HDI6886175.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNGYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(36613..37713)
                     /gene="qoxA"
                     /locus_tag="POH73_000187"
     CDS             complement(36613..37713)
                     /gene="qoxA"
                     /locus_tag="POH73_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HDI6886176.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            38018..38137
                     /locus_tag="POH73_000188"
     CDS             38018..38137
                     /locus_tag="POH73_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886177.1"
                     /translation="MERKRKHFNEEFAKSNLSLNNEKRKFVCSKSKHSFKKSS"
     gene            complement(38285..38602)
                     /locus_tag="POH73_000189"
     CDS             complement(38285..38602)
                     /locus_tag="POH73_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HDI6886178.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
BASE COUNT        13596 a         5561 c         6541 g        13273 t
ORIGIN      
        1 aatttgtatg tgtttgaata tcaagcacaa atattattat aacaagatta agttaatttg
       61 caatagatac cttaaaaaaa tatgcagaac atttacacta tatttataaa acattccgca
      121 tattttaaaa ataaacaatt tatttatctt caaataaaga atagcgtgtc aatgcttcaa
      181 acacattcaa tgtggcttta ttataatcta ttggttggtc gggatttttt gatatcatgc
      241 gagattgttt atctaccatg taatagtaaa aaatggaatt gttatattgg tgccataaac
      301 tgtggtatga aacatgaaga atgattgatt cattattaat aaatgtcact ttacatttac
      361 gattttttaa ttctttaata ctttcgatat aatgcatatt taaccaaata ttttcatttt
      421 gtctgtcaga atgtgttggg aaaaaataag ttggaaataa tggtgttagt aaaataggtg
      481 gtttactaga aatgccagta atgcggtttg tctctgcttt cttgccaaga taagtattac
      541 cataaaattt gcaagagcgt tcaatgattt tctggacttt aaaaggattt tgaatacgcg
      601 ttttgtcaaa ccgaattatt tcactaccat ttctttgatc gtcatcatca aatgctggtc
      661 gaataaccat gtctcctttg cgtatcacat aaatattttg agaatacata agcaaaacct
      721 cgctttatta agttttaaac cattaatata aaaatgcaat taatgatata aagatatttt
      781 ataataaaat tctgagttgt caagttatta ttttggtgaa aatgtacaat aatttttaac
      841 taatagtcag aatattcatt gatttcaaaa ttagaaaaat gtatacttat tataaattaa
      901 agaaaggtag tggtttctat gaaatacaat actaatgtta aacatacaac tttagaagcg
      961 tttgtcacaa ccgtcaatga tttgggtatt gaattaatta tcaatgaagc acttcgagag
     1021 gtaagaaaac gacagctcat agaacttata gatgacgcac tcgtcaataa agatgaagca
     1081 gcatttaatc aatatatggc agaatacaaa aatttggagg catttctcgg tgaataacat
     1141 tgattcgaat tccataacgc aatcataaaa ggactattct taacatctca tacatgtaag
     1201 aatagtcctt taatcgttta tgacattaat ctaattaatt catgtctatc tatgtcaccg
     1261 aaatagtgat ataaatcata ttctgatagt gcttcttcta tgtgctcaaa gtcgtgtaga
     1321 cagccaacta atgcattttc aagatcagtg acatctccga caccaaagaa atcaccgaaa
     1381 atttttgcat gttcgatttt acctcgttta acatcaaact tgatttgtac aaatcctttt
     1441 tcaaattttt cctcgcgttc aaagttatat ttagggtttc ttccataatt ccattcccaa
     1501 gttctatatt tgtcgttact taacttttca atattttccc aatcttcatc cgttaattta
     1561 tattcttcta cttcagtttc tccaaagata gttttcaaga tgattttttt aaattcttca
     1621 atttctaaag ggtcatttaa aaattcttga atgtttgcta ctcgtttacg aacagattta
     1681 ataccttttg atttaatctt agctggatta acttttagtg cattctgaac ttcgtctaaa
     1741 tcactattta acatcaacgt accatgacta aacattctat ttttaacttt aaccatggca
     1801 tttcctgaga ttttcgcttg tccaacttga atatcgttac gaccagttaa ttcagcattt
     1861 acgcctaaag attgtaatgc ttgaacaatt ggttcagtga atttttggaa attgtggaaa
     1921 ctgttaccat catcatctgt tataaaacta aagtttaaat tgccagtatc atgataaaca
     1981 gcgccaccac cagaaattct tcttactaca tcgatgttat gagcatctat atatgtctga
     2041 tttacttcct ctatcgtatt ttgattcttt ccaacaatga tagatggtct atttatgtaa
     2101 aataaaaagt aactttcttc tgctggtaaa ttttttaaaa catattcttc cattgctaag
     2161 tttaaagttg gatctgtaat attattatta ctaatgaatt tcattacaat ctctccctta
     2221 tatctatata tattctctac ttatttatgc cttaactttg ctgaaaatgc caattatata
     2281 ctcacaaatc gttctaaata tcggcttaaa gttacatttt attatttttg attgttaata
     2341 gtttatttgt taagtgaatt ttagtacaat ataatttagt tatgatttaa ggaggactta
     2401 aaatgactgt tgcagaagtg ggtaacattg ttgagtttat ggatggatta agaggtcgtg
     2461 ttgaaaaaat caacgataac tctgttattg ttgacttaac aattatggaa aattttaatg
     2521 accttgattt accggaaaaa actgttatca atcataaacg atataagatt gttgaataag
     2581 aaggtaagtt ataatgaata aaatctcgaa ggctttaact tggtttatta taagtttcat
     2641 tatatttcat ctcatattat ttattatgtg gggcgaacac caagaatact ggtatttata
     2701 tacaggtata atgctaattg ctggtatcag ttatgtgttt tatcaaagag atattgaatc
     2761 taagcggttg cttacatcaa ttggtgttgg tattattacg gcaattattt taattatgct
     2821 tcaactttta ttctcactta taacttctaa tttaagttat agttcattaa ttaaagaatt
     2881 agcaagaaca ggtgtcaatt ggaagtggca aatgttagta actttacttt ttgtcattcc
     2941 atgtcacgag ttatatatga gaactgtttt acaaaaagaa ttaacacatt tttcaatacc
     3001 gaaatgggta gctatattaa taactgcaat ttgttctagt tcattattta tttatttaga
     3061 taattggtgg atagtaacat ttatatttgt tgcacaagtg atattatcat taagttatga
     3121 gtatactcga cgtatagcaa caacatccgt tgcacaaatt gtagctatta tattgttatt
     3181 aatttttaat gcttaaataa gcttttctta gacgtctgtg ttctaagaaa agcttttttg
     3241 ctttagaata gcaatacgaa gattcgaggc atgcttcatt atagtgtaca cgtttttaac
     3301 ggcaagctgt tattattgag attaggtttt agtcagtttt tattactaaa tattttataa
     3361 aaacagtata atattttgcg agcaagttta attgatggtg gctatctgag taaagggggg
     3421 tggtgcctat ggcattactt aacacttttg aaaggaaaag cctattgtga tatctattgc
     3481 aaacgcatta catttaatgt taagtttcgg tatgtttatc gtcactttca ttggtgtaga
     3541 agtcgcaata attaatttaa acaataaaaa ataaccatca tttcaaaact ttgacaacga
     3601 atgatggtta aagaaaatct catttagtca ccgtcttttt aacgggctca ttaggcgaca
     3661 tgttcctgca tgtcgtcttt ttacattttt atagtaacat atcttttatt taattgtcta
     3721 tttttattag atgtgtaaca aaattttgaa gtattttccg tatattgcct gctaaacatg
     3781 tcagatttga aaggaaaatc atactgtggt agaaatcaat tatgattata tggtagtact
     3841 tataaataag tgatttctat tgaatatgta atgaagttca tatattaata taactttcat
     3901 tgatatagaa gtcgcaatga ttaatttaaa caataaaaaa caaccatcat ttccaaactt
     3961 tgacaaagaa tgatggttaa agcaaatctc atttagtcac cgtcttttta acgggttcaa
     4021 taggggacat gttcctgcat gtcgtctatt tatgcactgc gcaatttaat aataatacaa
     4081 ttatactttg atgtccattt aatataaagt ggttgaaatt tgcagcaaat aaattaacct
     4141 tgactatttt cagaaaattc tgtataaata acgaaaaaga gatatggatt aaatatgaag
     4201 aggtgttttt atagaagaat aaaatttttg gcactatatt atatttatct ttagcacttg
     4261 gattatcaac agcagcttat gcatctacag aatacgctga aggaggcact tggagtcacg
     4321 gtgtcggcag taagtatgtt tggtcgtatt attatcatgc tcataaaggt catggtgcaa
     4381 cagctatcgg aaaatataga tcatttagtg gttatacaag agccagtgta aaagcaaaag
     4441 catcagctac taaacataat ttgcgggtca atagagcgta ttataacatt tattaaatac
     4501 taaaaaatca tagaaagtaa acaacgcaag aggtacaaca tgaaatggtt caaattaata
     4561 cttgatgtta caatcttcat tctgattgct atattgttat ttgtttatac atataaagaa
     4621 aacgaagaaa tattgcctga tactaaatac cctatagcgg taactgactg gaataaaaaa
     4681 tatagtaaaa atgagattta taaacgtata aatcaattcg ctaaaaatga gaacgtagca
     4741 atctataaat caacttcaaa ttatacaaac aaaaacgtag ataaagatat atatgtattt
     4801 aataaatcaa aagcaacaac tatcactcct tttaatgcta aatataacat tcattattta
     4861 agtgacgacg aattattaaa aaaagatatc aaagggagtt attttgtaaa agacaaaaat
     4921 tttgacgtga ctaaattcat aaatttttta aaagaatatg gtgttactgc tgaatcatac
     4981 aaaatagatt atatgatgat tgccgttggt gtcattaaac aaatgaatat agtagtgcca
     5041 ttatcctcac ttttaatcgt ttattttatt tattatattt tcgaaaagaa cattaatttc
     5101 aaagcgtatg cgattaagta tttaaatggt tttacattaa gaaaaataat tttcgaaaat
     5161 ttttcaaaga aatgcatgta ttgggtaacg ttaatcataa ctcaaattct tttaactaca
     5221 agtgtacttt ggatattaaa ttacacgggt aatttagatt tattcatatt aagattagtt
     5281 ctgctttcat gtcttttcat tttaacgatt agtgttatta atttatggac tttcttaatg
     5341 ttactaaatt taaatattgc caatatgatt aaaggtaagc agcactttaa aacaattcgt
     5401 tttattaata cagtttgtaa aagtgttctc ttagtactaa tagctagtgt aatgatagaa
     5461 aatactagtg ttattaaaga tttgaataaa ataaaagaaa ctgagaaata ttggaatgta
     5521 ttagatgatt attacacgat tgaatttgca ccttatcacg aaacaaaaca aagtttgatt
     5581 gataatatgg tgcgatcaga acaattagta aaggctagtg aagcagaaaa taatgcgatt
     5641 ttattcaaac caaagggtga ctccgttgac aatgacaact tttcacctga tgaggggaat
     5701 gtaatattag taaataatca gttttggtcg atttatcaca agcagtttca acctgatatt
     5761 ccaataaaaa atcaaaaaaa taatgtcgaa gtaattattc cacaaaagtt tcatgcaatg
     5821 cgtaatgaaa tcaatcaagc atatcattca tggtttgaat ttgtacaaaa taaaaataat
     5881 aaagagagta agttatctat acagtttatc aacaaaaatg attatcgaat ttttacattt
     5941 gatgcacgag atagtcgcca tttgtcattt atagaggcgc caatcattgt gaatgttcag
     6001 gcatcagatt tatcgaagga tttttattat gccatgatca gtcaaggcgg gtatttattc
     6061 aaaaattatg acgcgctagt aaaaaatatt gaaaagtatc atcttgatgg ggaaatcagt
     6121 ggaataacca attataaaga tagcgtgatg gaaatgtatc atgaaaacaa tttgaaatta
     6181 acagtactca acttttcaca aatcattatc gcaatcattt taataattat tattttattt
     6241 gatgtgaaat attattttga acaacatcga aaattactcg taatcaaaaa gctatatggt
     6301 tattcaacat taagagccaa ttaccaatac ttattaataa ataatatagt tgttgttttt
     6361 attggaatat tgacgaatgt aattttacat tctcactata taatgatgat atttgcaacg
     6421 attattgtcg ttcaaatatt attgcagatt tgcagcctat actatcatgg ccgacgtttt
     6481 aatgaagtta tcaaggagtt ttaactatga aatatatcat tgcaatatta ttaggattaa
     6541 tcgtatctat tactattgct tttacaatca tacatcatcc tatacttgat cgttttaatc
     6601 ctttcttaaa aacggaatat agttatgcca aagtgccaaa aggtacgcaa caatatgtta
     6661 atattacggc ttatagtgaa agaggggaaa agcttgatta taaattaaca tttaatggat
     6721 tttctcctag tagaacgtat gtcgaaataa agcataaagg gcaatatgtt atatcaatca
     6781 catatgttga aaaagatgat acaccaaaag aggtaagaca agaatgatag aattaaaaga
     6841 tttaaccata caaaaaggta atacacatat tttgaagaaa cttaatttga aatttcaatg
     6901 tggaaaatca tatgcactta ttggtaaaag tggatgcggt aaatctacat tattaaatac
     6961 tattgctgga cttgaaaaaa caggaaaaca atatgtctat tttaatggtc aattagaaca
     7021 atttaaatct aatttttaca gagataaatt agggtattta tttcaaaatt atggattaat
     7081 cgttaatctg acagtaaatg aaaatttaga tattggatta gcatataaaa aaataagtaa
     7141 gaaagaaaaa gaacaattaa agatacgtta tatagaacag tttggtctgt caaacagttt
     7201 aaaaagaaaa gttcatacgc taagtggagg tgaacaacaa cgtgtcgctt taattagaat
     7261 gatgttaaaa gatccgattg ttatgttagc tgatgaacca acgggtgcgt tagatcctaa
     7321 aacaggacag atgattattc aatcattatt tgatttgatc gatgaaaata aagtgctgat
     7381 tttagcaaca catgatatgg ctattgcaaa tcaatgtgac gaaataatag atttagaaca
     7441 gtatagaaaa gtagcatcta tgtgatatcc aaaagataaa attaaaagcc aatcaattgg
     7501 gaatgttaat ttcaacactc catttgattg gcttttataa tgcgttgcta tgttatgaaa
     7561 gtaacaacgg caaactgaaa aaaaacaata taataatatt taatacgagt agtaataaat
     7621 gtaattgatt ttttgtttta atgtagcaaa aaacaatgac taaagatagc agcaatatca
     7681 tgattgcacc tgggaagaac cataatgctg aagctacata ccaaatcatt ggtaacgcca
     7741 taataataaa agatataaga caagaaagga taccaataat attataagtt ctagtcaaaa
     7801 ttacacctcc aattttcatt ttaaaatgac atgcaaaaaa caatcatttt aaatacatca
     7861 tacaataaaa ataaattata tgatagatga atttgaaatt aaattgaatt ggcttatcat
     7921 tcgtaaacat tattatgacg taacaattct tataagtgga acgagtgtaa ttataccatc
     7981 tgtatagaga gtcgtatcaa tcgtaaaatt taatctatgt tgttattgat ttttatacta
     8041 caaataccgt tctaacattg tgtgaaatat tatttttcac aaataaacga aattaaatat
     8101 tttaattatt gtatatatat agttgctcta aatggttgcg gagtataaaa tatattttgt
     8161 taaatatttt attaagaggt gcgctatgga aaaggggatt ttcaattacg ataatgcgaa
     8221 tgtattaaaa ttagatacta atcagttaaa tgaaaacata aaagtcattg acgatatatt
     8281 taaaaattat gagcagatag agccaactat agaaatcgaa aatggaaata caaaattaaa
     8341 attaaatgga tattttattg cgtcaattat aagcccatta aatttaaata aacttaataa
     8401 tttatatgtt gaagaagaat tttatcatac atataacgaa ttaattgtta aatatactga
     8461 ggtaaaggaa taattataat gaaaaaaata tttatgatgg tacatgagtt agatgtcaat
     8521 aaaggtggta tgacctcttc gatgttcaat agaagtaaag agttttatga tgcggacata
     8581 cctgctgata ttgttacttt cgattacaaa ggaaactatg atgaaattat taaagctttg
     8641 aaaaaacaag gtaaaatgga tcgaagaacg aaaatgtata atgtatttga gtattttaaa
     8701 caaatttcaa ataataaaca ttttaagtct aataaattgt tatataaaca tatttcagaa
     8761 agactaaaaa atacgattga aattgaagag agtaaaggta tttcaagata ttttgatata
     8821 acgactggta catatattgc ctacattaga aaaagtaaat ctgaaaaagt gattgatttc
     8881 tttaaagata ataaacgaat tgaacggttt agttttatag ataataaagt gcatatgaag
     8941 gaaacattta atgtagataa taaagtttgt tatcaagtat tttatgatga aaagggatac
     9001 ccatatattt caaggaatat taatgctaat aatggtgctg taggtaaaac ttatgtgtta
     9061 gttaataaaa aagaatttaa aaacaattta gcactgtgtg tttactattt agaaaaacta
     9121 ataaaagatt ctaaagatag tattatgatt tgtgatggac cagggagttt tccaaaaatg
     9181 tttaatacaa atcataaaaa tgctcaaaag tatggcgtta tccatgttaa tcatcatgaa
     9241 aatttcgatg atacgggtgc atttaaaaaa agtgagaaat atattattga gaatgcgaat
     9301 aaaattaacg gtgtaattgt attaacagag gcacaaagat tagatattct taatcaattt
     9361 gatgtagaaa atattttcac tattagcaat tttgttaaga tacataatgc tccaaaacat
     9421 tttcaaactg aaaaaatcgt aggtcatatt tctagaatgg taccaacgaa gcgaattgat
     9481 ttgcttattg aagtggctga gttagtcgta aaaaaagata atgctgttaa atttcatata
     9541 tatggagaag gatctgtcaa agataaaata gctaaaatga ttgaagataa aaatttagaa
     9601 agaaatgttt ttcttaaagg atatacaaca actccacaaa aatgcttgga agattttaaa
     9661 ttagttgttt ctacatctca atatgaaggt caagggttaa gtatgataga agcaatgatt
     9721 tctaaaaggc ctgttgttgc ctttgacatc aaatacggac caagtgattt tatagaagac
     9781 aataaaaatg gttatttaat agaaaaccat aatattaacg acatggctga taaaatacta
     9841 aagcttgtta ataatgatgt tttagcagag gagtttggtt cgaaagcgag agaaaacatt
     9901 atagaaaaat attcaacgga atcaatatta gaaaaatggt taaatctttt caatagctaa
     9961 atgaaaaaat cctaggtatt catgagtacc taggattttt acgcatgata tatacaaaaa
    10021 aagagaggat agtcctctca aataatagtt aataaaattg tagccatttc ttccagctgt
    10081 aactactaat acaagctaag ttcattacga tgtgtatgat ttcaaattca taaccactta
    10141 catatcctct tgtaagttta agtgtgaaga ttgagcctaa cataacacaa atataaaaaa
    10201 cattaacata aggcgttaaa aagccgataa taagtgctaa acctccaaca acctcaccaa
    10261 ttgacatcaa tactgcgatg gtaggtggta aacctaaaga ccctaagaag tgaattgttc
    10321 catctaaatc tacaaatttc aatgtgccgt gtactaagaa tgatgttgct agcatccatc
    10381 taattaatag catacctaat ttcatcatgt cacctccaac atacattttt ttcttaaaac
    10441 atagtccaaa ttatatttgt aaagaagtgg aaagtaaaga ttatttataa aaataattat
    10501 taaatattta ttaaatgttc tattgtattg aacaattgtt gtagaaagat gtttaagtca
    10561 tcagttactt tttgtattta taaattaaga ttagtacagc atagtgcaaa gtttataggt
    10621 aaatgaatta attatggcat caaaatatac gtatcttgaa cactgttgtg gaggcttagt
    10681 agtagtgaga acaaacagaa tactacttta agcgatatga ttaatattaa ccagtgcgtc
    10741 atcatcaaaa ttagaggcca tttgctgttt ataatacgaa gatttgataa tcaactatga
    10801 taagaaaaaa taatgaccgt ttaaatatat tataagttta ttaaatgata aaataataat
    10861 atagttctct ataaaaattg tttggaggta aatgtgtgaa taggaatatc gttaaattag
    10921 ttgtgtttat gctaatctta gttgtagcag tagcgggttg tggtcaaaaa gatactgaag
    10981 agaaaactga aatgacgacg ataaaagatg aattaggaac tgaaaaaatt aagaaaaatc
    11041 cgaaacgtat tgttgtatta gaatatagtt ttgctgatta tttagcagca ttagatatga
    11101 aacctgttgg tattgcagat gatggtagta ctaaaaatat aacaaagtca gtaagagata
    11161 agattggggc atatgaatcg gttggatcta gaccgcaacc gaatatggaa gtgataagta
    11221 aattaaaacc ggatttgatt attgcagatg tcagcagaca taagaaaatc aaatcagaat
    11281 tgagcaaaat tgcgccgaca atcatgttag ttagcggtac gggagattac aatgcaaata
    11341 ttgaagcatt taaaacagtc gctaaagcag ttggcaaaga gaaagaaggc gaaaagcgtc
    11401 tggaaaagca taataaaata ttagcggaga ttagaaagaa aattgaacag agtacgttaa
    11461 aatctgcatt tgcatttggt atctcaagag caggtatgtt tattaataat gaagatacat
    11521 ttatgggaca attcttaatt aaaatgggta ttcaacctga agtcacaaaa gacaaaactg
    11581 cgcatgttgg tgaacgcaag ggtggccctt atatttattt aaataatgaa gagcttgcca
    11641 atatcaatcc aaaagttatg attttagcta cgaatggaaa aacagataaa aatagaacga
    11701 aattcattga tcctgcagtt tggaaatcat taaaagctgt gaaagataat aaagtatatg
    11761 atgttgatcg aaataagtgg ttgcaatcaa gaggtattat ggcaagtgaa agtatggcag
    11821 aagatttaga aaaaattgca gaaaaagcaa aataaaaata cagcgccact cgtaaatcat
    11881 ataagagtgg cgctgtattt tttatttatg tttattatct gtcggatgtg atgatttacc
    11941 tgaaagttta tttcgaataa atttaattac ataaccgaca aggattgttt taacaattct
    12001 tttaatgaat tgacgcatcg ttacatacct catttctctt tatcttatgt actatatacc
    12061 cattcatata tgctttttaa acgtcattgt cacaatttaa tttttaggga atataatata
    12121 accgtcttta tctgcttttt tagtaaaaat gacaataatg gcatgtatta ttgaaatgat
    12181 ggtagggata cctgtccaga aaaataataa gtgaaaaaga ccttgtccaa atttatcagc
    12241 ataaaattta tgaataccta aacctccaag aaataatgca acaataacat aaatggcttt
    12301 attgactttc atttgtaaat cctccttaat tataattcta cttaaattcg ttgtgaaaac
    12361 caatctttct aactttagaa ttttcaaact ttctaaaatt ataagtatat ctttttaaaa
    12421 taagctagaa tttctatata ataaatgtta ataacgtaaa agggaatgat gacatagtga
    12481 tacgtcaagc acgtccagag gaccgatttg atattgcgaa gttagtttat atggtttggg
    12541 atgatatgga attagaattg gtaaagcatc tacctaaaga catagtatta gatgcaattg
    12601 aaaaaagctg tgttgatgca acatatagaa ctttttatca gcatgtttta gtttatgaaa
    12661 tagaaaataa agtagcaggt tgtattatta gctatagcgg tgaaaatgaa ttgaaatatg
    12721 aaaaagcatg ggaacaactt gacttgccag aagaaataaa acaatatggc acgccattac
    12781 ccgtaaaaga agctaaagac gatgagtatt atatagaaac aattgcgaca tttgaagcat
    12841 atagaggtag aggtatcgcg acaaagttat taacgtcatt acttgaatca aatacacatg
    12901 ttaaatggag tttgaattgc gatattaata acgaagcagc attaaagtta tataaaaaag
    12961 taggctttat atctgatgga cagattgaat tatataagca catgtatcat catttaattg
    13021 ttaaataaaa aaactcgaca gttcgatgta agtcgattgc cgagtagaat catcttatat
    13081 taaatgcctg caaataatgt actaatataa atacctagtg catataataa accgaaaaat
    13141 gtatttgttt taccagcagc agccattgct ggcatcattg taggcggtgt atcattcttc
    13201 ttgaaacgtc tgataacttt aacaggcatt gggaatgata acaacgcaag taagtaaaat
    13261 aatgagccac caggtttaat aatgatcgta agtacaataa atgcataagc gataaagtac
    13321 atgattgcca taaatgttaa agaagcattt ttacctaata gaatgggtaa agttttacga
    13381 ccacttgctt tatctttgac acggtcgcga atattgttgg ccatattaat taaaccgata
    13441 gtgattacta taggtacact taaccaaatt acatagcttt gaatattgcc agtttgaata
    13501 aagaatgcaa taacgataat aaacataccc ataaatacgc ctgagaataa ttcaccgaaa
    13561 ggcgtccatg aaatagggaa agggccacct gtatataggt aaccaacagc catacatact
    13621 aatccaactg gtaataacca aaatgaagag ttagcagcta aaaacaaacc taatatcgct
    13681 gctaagatgt aaaatgcaat ggctaatcgt agcacaagct ctgggctcat accgttgcgt
    13741 acaattgcac caccaatgcc tacagattca tgatcatcaa ggcctttttt ataatcatag
    13801 tattcgttaa acatattagt tgctgcttga ataagtaagc atgctagtaa catggcaatg
    13861 aataggctga ttttaatatg atcttcgcta ccaagaaaat atattttaga tgctgctgta
    13921 ccaactaaaa cgggtactac ggaagcagtt aatgtatgag gacgcattaa atgccaatat
    13981 ttcttaactg tagaatattg ctgatattga ttactcattg aatacaaaac ctctttaaat
    14041 catatttttt ataacaaaga tattttaata gaaagtagca taaaggtcaa ttgacgtgaa
    14101 aaattgcaag ataagtttaa gcaattcttg taacacaatt atccaaaaat gatacaatat
    14161 aataatgagt gaaaaaagac gaagaaagaa gtgaaatgga tggctacggg cgtattagag
    14221 gacgatattg tcaaagagat atatgacagc tcaaaggaat gggtttcagt tgaagtgaaa
    14281 ttatcacagt cactagaccc gagcacatta tttcatctca ctgacaatga ggcaggagat
    14341 cgcttttata tgcgtttgaa tgataatcga acgtcatatt ttggctacaa agcaattcaa
    14401 ttattcaaaa ataattctaa aaataaacaa tctattttta aagactggga aaaattaaaa
    14461 cataacatca catttataca tccgcaatct gagaaacatc atcttcgagt tgttggaggg
    14521 ttccaatttt caagtcataa atcagatgat gaatggcgag agtttggact aaatcatttt
    14581 gtattacctg aagttttaat ttcaacagat aataatggga catttttaac ttatacagtt
    14641 aaaagggaat gttttactgt tgaggcattg aacgatttaa cggatttgtt caacaatata
    14701 tcggatatag atgtggacga gcaaattggg gaaattacta gaaatgaaga tatttataaa
    14761 gatgactggc gtcaacttgt agtagaagca atagaatcta ttaataatga agaaaaaatt
    14821 gtactagcac gtagacggtt aataaagttc gataaagata tcagtattcc atatattcta
    14881 aagcaagcat attctaaaga aaaaaacagt tatatattct tgttagaatc acaagattct
    14941 atattctttt cacagacacc tgaacaatta ataaaggtca ataataaaat actatcgact
    15001 aaagctgtag caggtacaat taaacgttca caagatgagg acgaagatac aaaaaatgtt
    15061 gaagcatttt taaaagataa taaaaactta atcgaacatc gatttgttgt tgacagtatt
    15121 ttacatgata ttaaacctta tatcactgaa ttacattatg ataagacgcc taaaattcta
    15181 aaaaatgatc atttatatca cttgtacact gaaataaagg cgccactgaa ggatgattcg
    15241 tatattagtt taattgataa tttacatcca acacctgctt taggtggcta tccaaaagaa
    15301 tttgcgatgg attttattga acagaaagaa tttggtacac gaggattata tggtgcgccg
    15361 gttggctata tagatatata tgatgattgt gaatttattg ttgcaattcg ttcaatgctt
    15421 attaagaaag cacaagcaac tttatttgct gggtgtggca ttgttaaaga ttctgatcca
    15481 gatagtgaat tggcagaaac gaaccttaag ttcacaccta tgatgaatgc attaggagtc
    15541 gatatgaatg ggaaatcata aagcagcttt aacgaagcaa gtttttacat ttgcatctga
    15601 gttatatgcg tacggcgtaa gggaagtagt tatcagtccg ggatcacgct caacgccact
    15661 tgcacttgca tttgaagcac atccaaatat taaaacatgg atacaccccg atgagcgaag
    15721 tgcagcgttt tttgcagtgg ggttaattaa aggcagtgaa agacctgtcg ctatattatg
    15781 tacgtcaggt acagcagcag cgaattatac gccagcaatt gctgaaagcc aaattagtag
    15841 aattccatta atcgttttaa caagtgaccg tccgcatgaa ttaagaagtg taggcgcacc
    15901 acaagcgatt aatcaagtaa atatgtttaa taattatgta agttatgagt tcgatatgcc
    15961 gattgcggat gatagtaaag agaccattga tgcaatttat tatcaaatgc aaattgctag
    16021 tcaatattta tatggaccac ataaagggcc gattcatttt aacttgccat ttagagatcc
    16081 gttaacacct gatttgaatg caacagaatt gttaacttct gagatgaaga ttttaccgca
    16141 ctatcaaaaa agtatagatg catcggcatt aagacacatt ttaaataaga aaaaaggttt
    16201 aattattgta ggagatatgc agcatcaaga agttgatcaa atactgacgt attcaacgat
    16261 atatgatttg cctattttag ctgatccttt aagtcattta agaaaatttg atcatccgaa
    16321 tgttatctgt acatatgatt tgctgtttag aagcggctta gacttaaaag tggatttcgt
    16381 aattcgtgtt gggaaaccag tgatttctaa aaaattgaat caatggttaa agaaaactga
    16441 tgcatttcaa atattagtgc aaaacaatga taagattgat gtctttccaa tagcaccaga
    16501 tatttcatat gagatttctg cgaatgattt ctttaggtca ttaatggaag acacgacagt
    16561 taatcgagta agttggttag aaaaatggca acgcatagag aaaaaagggc gtaaagaaat
    16621 taaatgttat ttggaacaag ctacagatga gagtgcattc gttggtgaat tgattaagaa
    16681 aacatctgaa aaagatgcat tatttattag taatagtatg ccaatcagag atgtagataa
    16741 cttgttattg aataaaaata tagatgtcta tgcgaatcgt ggtgcaaatg gtattgatgg
    16801 tatcgtatca actgcacttg gtatggctgt ccataaacga gttacattat tgataggtga
    16861 tttgtcattt tatcatgata tgaatggact attaatgtca aaattaaata atattcagat
    16921 gaatattgta ttattgaaca acgatggtgg cggtattttt tcatatttac cacaaaaaga
    16981 aagtgcaacc gactattttg aacggttgtt tggcacaccg acgggattgg atttcgagta
    17041 tacagctaag ttatatcaat tcgattttaa acgttttaac aatgtttcag aatttaaaaa
    17101 tgccacattg ttatctgaaa cttcgacgat ttatgaattg ataacgaatc gcgaagataa
    17161 ctttaaacag catcaaattt tatatcagaa attgagtgaa atgattcatg acacattata
    17221 attactatga agcaaacgtt gagaccaatc aagttttagt atttctgcat ggatttctta
    17281 gcgacagtcg tacttatcat aatcacatcg ataaatttac tgataactat catgtcatca
    17341 ctatagactt accaggacat ggcgaagatc agtcttcaat ggatgaaacg tggaattttg
    17401 attatattac gacgttgtta gaccgaattt tagataaata taaagataaa tcaataacat
    17461 tgtttggata ttcaatgggt gggcgtgttg cattatatta tgcaattaat ggtcatatcc
    17521 ctatatctaa tttgatatta gaaagtacgt caccaggtat taaagaagaa gcaaatcaat
    17581 tggaacgtcg ccttgttgat gaagcgcgtg ctaaagtatt agacatagca ggtattgaat
    17641 tatttgttaa tgattgggaa aagttgccat tatttcaatc gcaactagaa ttaccagttg
    17701 aaatacaaca tcaaataaga caacaacgat tgtctcaatc gccacagaaa atggccaaag
    17761 cattaagaga ttatggtaca ggtcaaatgc caaacttatg gccgcgcctg atagaaatta
    17821 aagtaccaac attaatatta gctggagaat atgatgaaaa atttgtacag attgcgaaaa
    17881 aaatggcaaa tttaattcct aatagtaaat gtaaattaat ttctgctaca ggtcatacaa
    17941 ttcatgtgga agatagtgat gaatttgata caatgatatt aggattttta aaggaggagc
    18001 aaaatgacta acagacaatg ggaaacactt agagaatatg atgaaatcaa atatgaattt
    18061 tacgaaggga ttgctaaggt aacaataaat cgccctgaag tacgcaatgc gtttacacct
    18121 aaaacagttg ctgaaatgat tgacgcattt tcacgtgcac gtgatgatca aaacgtttca
    18181 gttatcgtat taactgggga aggtgattta gcattctgtt ctggtggtga ccagaagaaa
    18241 cgtggacatg gtggttatgt aggtgaagac caaatccctc gcttaaatgt attagattta
    18301 cagcgtttaa ttcgtattat tccaaaacca gttatcgcga tggtaaaagg ttatgctgta
    18361 ggtggcggta atgtactaaa tgttgtttgt gacttaacga ttgctgctga taatgctatt
    18421 tttggacaaa ctggtcctaa agtaggttca tttgatgcgg gttatggttc aggatattta
    18481 gcacgtatcg ttggacataa gaaagcacgt gaaatctggt acttatgtcg tcaatacaat
    18541 gcacaagaag ctttagatat gggcctagta aatacagtgg tacctttgga aaaagttgaa
    18601 gatgaaactg tgcaatggtg taaagagatt atgaaacact caccaactgc tttacgattc
    18661 cttaaagcag ctatgaatgc tgacacagat ggtttagctg gtttacaaca aatggctggg
    18721 gatgcaacat tgctttatta cacaacagat gaagcgaaag aaggccgtga tgcgtttaaa
    18781 gaaaaacgtg atcctgactt cgatcaattc cctaaattcc cataagttat tttgaatatg
    18841 atattaagtc acttgcgtcg tttattagcg acgatgggtg gcttttttat tttataagta
    18901 tttaaaaggg catttttatt aattatcata aagataagtt agtttgagaa aaattaatag
    18961 agaaagaata aatgttaaag attattacaa tgaaaaaacg agctggtgag aaattgaatt
    19021 ctcgccaacc cgctttatga agtgaaatca tttaaattag ttatactaag cgctcataaa
    19081 caattggtcg cgaagtgcca ataccttgtt tggatgacgt ttgcatgagg atatgctgtg
    19141 tatcatcata atcaagaata taaatgattt cttcattatc aaaagatgaa aatttaatgc
    19201 gttgatgtgc cgtatctata aaaagaatat gatattgatt gtgacttgta tcatcgccat
    19261 gacgtataca aattgatttt tgtaattgat gattactcca attaccaatg aataaattga
    19321 tattggtttg ttctagatgt gtgagtttgg ttgtgtcgta aactaaattt ataaattgta
    19381 gttgatacat agttttacct gcctttaatc aatatctata ttacttttta gtaacctatc
    19441 attgaaccat accagttata atcacgattg aatgataaat gtaataggct tgaatctgca
    19501 tcttggattg ataattctgt atcccaagga ttccagtaaa taagtttttc ttggtcatta
    19561 attttagcat tagcaacaac tgctagggca tgtcctaaat gtgggtcatt aggattttga
    19621 gatacacttt gtgcaagaat cataataccc acattatctt ttgtaagttg atcaacttga
    19681 ttatatgatg gtacgccttc ttgataatga atatctctac cttgtgattt accgtattca
    19741 atcatttgat tagggaatgt tgcacaatta ggaaggtctt gctcacttac ttcagggtat
    19801 aatgtacgca taatatcatg tgcattataa gtgtctgtat ttttagttgc atttaataac
    19861 gctgccatac tgaatcctgc acaccatgag ttatcgaatt gttgttctct aattttgaag
    19921 ttttttagtg tgttttcata ttgaacttga tcttcttgta tcgcttcgtt ttcttcaact
    19981 tttgtaggtg ttactgtagt ttttagctct tgctttaatt gtggtgaaac tgttttagca
    20041 ctttcttttt ctttgatgtt attagctagt ggcgtagctt ttactaatct aactttgcca
    20101 tcttcttcaa aataaaatcc tttttcatca gtaagaacag tgatatttga atttttatct
    20161 ttaatttggt ctaaatcttt agcgatgaag tttgaaattt taacactgta attcatgtct
    20221 tcttttgatt tgtttaaatc atctttattt ttagggctaa gtgttaaagt ataaacaatt
    20281 ttaccgtctt ttataactgg ataatagtaa ctattatctt cttcgccatt aaatttataa
    20341 attttaaatg cttcaccaag ttcgtattta ccagtttttg cattactttg tttatctaat
    20401 gctttagcat aaccagcaaa ttgttgttgt gctagatctt ttactttttg aggtactttg
    20461 tcactcttaa cattaatttc tagctgttta gagtgtgaat cggctttggc tttattgtta
    20521 ttagcaaatg cgcctagtga taaaataagc attgaaacca ttatgatgct tataatattg
    20581 aatactctag ttttacatga actattcata taataccctc catttaagtc aaaatatttt
    20641 gcgttgttaa gtttttgctt ctatatacta aatccaaatt aagatgaagt ttatgcagcg
    20701 tcagggttgt ctgaattatt gttatcgcca ttgtccgggt tatttgggtt gttaggttgg
    20761 tcagggttat ttggattgtc cgggttgtta gggttatcag gattattagg attgtctgga
    20821 ttatcagggt tgttagggtt atcagggttg ttaggattgt ccggattatt tggattgtct
    20881 ggattatcag ggttgttagg gtggtcatca tttgcgaaat tgatatcttc aatattttgt
    20941 tttaagaagt tgcgtacatt ttcattaata aatactgcac cgttaaattg atttggaacg
    21001 ccaccccaat gaatgccaat gacttcattt ttttcattaa atacaggtga acctgagtta
    21061 ccaccagttg tacttaaatc atattgcatt gcttcacctt ttaagtacgt tatttttcct
    21121 ttactttccc acattgttgc gacaggttta tcaccaggat atcctgttac agtaatattt
    21181 tggttaactt gtgtttcagc attattactc attgttgctg gtttaactac ttcgccaata
    21241 tgtttgtttt gctcattagg ggagaattta acgattgcta aatcaccttc gcctgaatat
    21301 ttagtgattt gttcagcagt gaaaccacca ttaggataat tgtcttggtt aattgcagaa
    21361 gggaatgctt ttaaagcatg aggatcaccg tgcgtagcat ctacgacgtg tttatttgtt
    21421 aaaagtgtat ctttacctac aactacacca gaagcaataa atgtaccagt aggtgcttca
    21481 acttgaatat aagtaacagg tgcataatga ccattcgttg tatctgtgat ttggtgacga
    21541 tcgttatttg gtaatataac attagcgcgt tcacgttgtt ctaatggttt aaggttaccg
    21601 cctttttgaa tcttaggtgt ttgctgtttg tctgtctgcg tttgttgtgg atgattgtcc
    21661 atagcttttg aagataacgc atttgctgct ggagaactca caagtgtcgc tgttgtcaaa
    21721 gttgcaacga ataaagaact aacttttaaa aatttacctt tcatctaaaa acctccaaaa
    21781 aatttattta caagttaaat ataacactaa aaatttttaa gtcaataaga atatataaat
    21841 aaattaaaaa gtttctgtaa agattagagt ttacaaaaac attaagagaa ttaaaattga
    21901 attgtattat actttattcg attgtattaa attggtaagg tcgtcaattg tttgcattta
    21961 tctaaaagaa taaataagaa aggaattgag agcgaagaga aagtatttgt ttataataat
    22021 aaatagtaac gtttgaataa taattgtttg gtcaaaggta gattaatatg aatgctgata
    22081 tattatttga gtgcttaagt ttcgaagaac aattatcagt tagcatatta aaagcttttg
    22141 atagaatcat tttgagtgag gtgtttaaag gagaggaata taactcggta gtatttacag
    22201 tctctttaga gtattattta aaacatatag aatataaaaa actgaacgta ctatgaattt
    22261 cgttcacgac gtccagttca acatatcatt tttatttagt atttaaatat ttagcaagtc
    22321 tcttcatacc ttcttttaaa acatccattt cataagcata ggaaatcctc acaaatcctt
    22381 taccgaattc tgtaaaggat gagcctggaa cgattgctaa atgtgttgat tcaagtaaat
    22441 cgacacaaaa ttcgaaatcg tcatcggtga tatgcttaat atttgggaaa atataaaacg
    22501 cgccttcagg ttgagcggta atctcaaaac ctaatttagt taattcagat actaaataat
    22561 ttcttcgttc tacataagct tcattcatat atttaggagc ttctaatcct tcgttaagtg
    22621 ctgttataca tgctatttga gctggaacat tggcacaaat acaattatag gcgtgcataa
    22681 atgttaattt atcaatcaaa tattgaggtc ctaatagaaa accaattctt attcctgttg
    22741 ctgagtgtga tttacttaaa ccaccaatta atattaattg atcacgaatg tcttcaaatt
    22801 cagcgaagga tacatgttta ccactaaatg tattttcagc ataaatctca tcgctaataa
    22861 taaatatcgg atattttttt aatacattta cgatatttaa aacttcattt ctttttaaaa
    22921 ctacgccagt tggattagtc ggataattta acaagacagc ttttgtcttt ggagaaatat
    22981 gactttctaa cgcatcaggt gtaattttga attgtgttgc tgttgtatca atataaattg
    23041 gtttaccacc tagtacttcg atgagtggta tgtagcctgc ataaattggt cccggtatta
    23101 taatttcatc tccaggctct atgatagaac gtaacgttgt gtctattgct tcacttgctc
    23161 catttgtcac aataatttct tcaggatcat aggaaaaatg ataacgattt ttgaagtatt
    23221 gactaatagc ttcgcgagtt tctaataagc ctttattgtg agagtaagat gtcttgtcat
    23281 tgttaatagc atctatatat gcctttttca caacatcagg catagggaag tctggttggc
    23341 caatagttaa attaacacaa tcatccaaat tattcatacg atttgaaaat tggcgaatac
    23401 ttggtgctct taaaaattta gaattagagt ttaaagaaag tttcatcgtg acacctcaaa
    23461 tataaatcaa atattgtcta aaaatttaga aattaataat atcataacat acttttaaag
    23521 caaaaaaggg taaattactt aagctttatt tgaaaaatga aaaatgtcta aatacactgt
    23581 agtaactact tttcaaaatg aatgaagtgg ttacagttaa tgtacttaga cattatataa
    23641 ttatgagtct ttaaactgtg atggtctttg taaattaatt attggatttg tccatttaca
    23701 aacaaagttt gtagataata catatacgat gattacagat atacttatta aataaagata
    23761 cgtcattaat gatataggat tatcgaatgg gtaccattca aatcctctaa caatgccaat
    23821 aattaaacca tgtaataaat atacgtatag cgtacgactg ccaatataag tatataattt
    23881 tttctttgtt gacattaaat ttagaaacgc agtcattgcg attaatataa ttccatataa
    23941 tataagtcgt ttaaaaggac tgaatacact ctgtccttca ttttcaagtg aagtatatgg
    24001 tgaacttcct aataaccaat ctgcattgat aggatgaatc acgtaaatga taaaaaataa
    24061 aataaacgta atgatagata ctggtattag ttttttattt ttaaaaatag ccgtatgttt
    24121 tttggtgaaa atgtagccta ggtaaaatat tgggaaaaat acgattgtcc ttgaaatgct
    24181 taagtagcta tcgatgttat ctgaaaaacc tgctccaata gatataataa ttgaaactga
    24241 tagcacttta tatggattaa atcttctaac tattactaaa atgacatgaa agaaaaatag
    24301 cgtgatcaaa aaccataacg caaatactgg gttaaaagga tcaagttgta attcgtcact
    24361 tttacctgtt aagaaataat atattgaaaa gaatgcaaaa aatatcatat aaggtactat
    24421 caagcgtttt gaaatttttt ctaaatagta tggtttatca atatttttcg cgaaataacc
    24481 agatataaac aaaaatgttg gcatatggaa actataaata actaggtata atgctgataa
    24541 atatttatcg ccactagtgt agggttgtaa catatgtcca aatacgacta ataatattaa
    24601 aattgctctt gcgttgtcaa aaaaataatc tctctctttt aatgaagtca tattttatct
    24661 cctttatgtc cataagactc ttactaattt attatatttt gtattagtta tgcaacaaat
    24721 ttgtctattg tgtcgaatag tattgtaaaa attataaaaa aagcatataa tattataaat
    24781 aatatgtagt ggagtagaac aacatgtata aacaacttga aaaacttatt acactgacta
    24841 acaatgactt aaacttagtg aatagaagat ttggacaacg cacggatatc acatctgaac
    24901 agctagaact tctccgtatt ttatttaatt acgatcgctt atcacagtat gatttgacga
    24961 tgaagattag tagggaacaa tctatagttt caaggtggat taagaaatta gttttgaaag
    25021 ggtacatcac aagtcaacaa tctagcgaag atttaagatg taaagaatta attttgactg
    25081 atcaagcacg tacattaatt tcacaaataa ataatgcacg ttgcgaattg attgaagcaa
    25141 gatgtcaatg tttatcggaa gtcgaattag acaatttaaa tcaattactt gataagttaa
    25201 atcaacgacg catatcgttg taacacggca atagcaaaca cataatttag tgtcattgtg
    25261 cacacaacaa aaattgaatc atgaattaca agcaaaagta gcggtgattg ttaaaattga
    25321 tggtaaacaa tcaccgctat ttttgcttgt gtatgtataa aaaagggatc aaaggtcatc
    25381 ccccatgatt gatagtgggg ggatgacttt tgatcctatg ttcatgttgc ttatttatat
    25441 tgtgggatgt cgaagtattt gccgacttcg ccaattttat catagtagcc tttgatgatt
    25501 ttagcattga tgttagccca atctacatct gtagcatatt ggtgtgttcc tggatgtgca
    25561 ggattccatc tcattttgta aagcgtattt tgaccagctt taacatatga gttgccgatg
    25621 aatttagcac caccaacgat tgcttttgat actgtgtccc aaccagcttg tttagcatat
    25681 ttaatacctt cacgtaaagg atcgttatca tatgcagcaa taccaaatac gttatggtat
    25741 ttcgtgttag agttagttac aactttgttg ttcactacat ctgcaccttt tgctaattga
    25801 gaagtaccgt tacctgtttc taatagggca tgtgagataa gataaacttc attaatgcca
    25861 tacatttgag cagctttgtt aaatgcagca ccttggtttt ctaatacacc tttacctttt
    25921 aagaattgat taattttatc aatagaaata ttttgtggtt ggtctaagcg taagaattga
    25981 tattttaatg ctggatcttg agctaaacgc ttcgtatcca ttgcatgctt aacatcatta
    26041 aagttagcat ctgtccactt acctggtaca cgttgtactt gtggtttata ttgtaaacca
    26101 gcttgtattt gagcaacttg gtttaatgtc atacctattt gattatactt aattaattct
    26161 ttagctaaat cagttgattt aatccatgct aatttaccgt tagataattt accatagtac
    26221 caagtttgtc cattaatgac ttgttcttta acaactgcga atggttgttc attaaatgct
    26281 tttaatgagt atttagctgt atcagaattt ggtgttacat agtaataacc attaccattt
    26341 ttaattacat aagtgtagtt ataatctttg gcagctgatg tagttggttt aacagcagtt
    26401 ggtgcagtta aatcttttga atttacccaa ccagtgcggt tattaatagt accgtataaa
    26461 taaacatctt tgcctacaga tacttgtttc gttgcattaa atgtaccttg agcaatgtta
    26521 ttgcctgtta aaatgacttg gtttttagta ccccaaggaa ccattgataa gccgttattt
    26581 gatctgttaa cagtatattt ttgagtcgtt ttaacttctt tgcctaagtt ttgaacattt
    26641 aagtctttta cattgaacca acctaatgga atattatgac ttgtattatt taataatacg
    26701 tatgtttcat taccatgtgc acgttctttt gttacataga atgtacgatc cgcatatttt
    26761 gcaccgtttt tcgctgtttt ttcataaaca gaagcacgaa taccagtgtt gtttggttta
    26821 acttgagcaa ttttgctaac tgtttgagtc gtttgaggtt tagtaacagc ataagctttt
    26881 acagcagttt ttggttgtgc tacagctttt ttaggtgcag caggtacagc taaatatgct
    26941 ttacttatcc aaccagattt accatttaca gttccatata aatagataga tttatcaatt
    27001 tgttgttgct tagtcgcttt aaaagtttgg ttacctgtac cagataccgc accagcttct
    27061 tgtttataag taccccaagg tactgaatat aatttagtgc ctggttttac tgtataagtt
    27121 tgcattacat ttacaggtga ttttgcattg ttataaataa cgtcaccttg tttaacccaa
    27181 ccaattaaag ttggactatt gtaatcttta actaagtaga atttgtttcc acctagactt
    27241 gcttcttttg ttacagcaaa tgttttttga acttctttcg ttggcttacc agttttgtca
    27301 taaactgtag tgaataagcc attgttttta gcattaattt gagcaacacc gtttaatgat
    27361 gaaactgtta atttattatt tgttgtaggt gttgatggct taggtgttgg tgtaggcgta
    27421 ggtttagcag tatcaactaa atatgcttta cttacccaac cagatttacc attcacagag
    27481 ccatataaat aaattgattt ataaatttgt tgttgctttg aagccttaaa tgtttggttt
    27541 ccagaaccag acacgctacc agcaacttgt ttagatgtac cccaaggtac tgtataaagt
    27601 ttcgtaccag gtttgattga atatgcttga tttacattta caggtgattt agctgtgttg
    27661 taaaccacat cgccttcttt aacccaacca aatttattac cagaattgta atcttgaaca
    27721 agatagaatt tttgattacc taatgtagct gttttagata cagcaaatgt tttttgaact
    27781 tcattagttg ctttaccagt tttgtcgtaa acagtagtat ataaaccact atttgtaggt
    27841 ttgatttgtg cgacaccatt attagcagca actgttaatt taccagttga tggtgttgat
    27901 ggtttcgacg gtgttgatgg ttttgatggt gtagtagggg tagttgtaga ttgcgtaccc
    27961 catggcgcca ctttacccat ttttattaaa tatttttcat taattaagtc atatagttga
    28021 tcataactat aattatgact tcttaaatat ccatgtggat cggcatggtc cgtaccacct
    28081 aaatatttac ttacagcgta gtgagtccat actgtaccat ttccatcata ttcagcacta
    28141 tcaggtttta aaccataata ttgtaattgt gtagctgcat agtcagcata gttattcatt
    28201 gaacgtgcaa atgaagcata atcgtgtgtg tgcacgattt caacattgat gaatctaggg
    28261 ttaccgactg caccgacacc ccaagataag taatccgttg gtgctgtttc gattatacga
    28321 tccccatcaa caaatgcatg tacgaatgcg ttttgatagt tgtttttcat ataactaatt
    28381 tcgccattta tcgtcgaacg atcattagct gtatcatgaa caacgatacc ttcaggacga
    28441 cctacaccgt tacggtatgc gtatttaggg aagtaagatg tataatcttc ctctatctta
    28501 ggtgctttta aattattttt acgaatgtaa tcattaattg atgagtttac ttgcggttta
    28561 tattttggca aactcgtttt aggtgctgca gcggctgatc ttggttgtgc tgaagcacta
    28621 aaagtagtta cttttggtgt agcttcagtt ttagctttag gtgcaacagg tgttgctttt
    28681 ggtgctgcgg ctttatattg cgtttcaaga gcagcaggtt tagcagctga tttaattaat
    28741 tctggattaa tttgattttc tgaattatca tcttcatcat caactaaact atatccagca
    28801 cttgtaacat tattgttcgt tttaggtgct gttgtgtttg ttgactttgt aacaggtttc
    28861 gcattatttg atgtagttga ttgagtcgca cgagtgtcac catttacttg tgtagtatca
    28921 accttttgat ttacttgagc attgcctgtt ttattcgctg cttttggttg gacaatagca
    28981 gggtcttgat atacttgagt gccagaaatg ttttgcgttg gattttttac ctcagctttt
    29041 gcttgttcag tagttgcttt aactttatta ctatctaaaa catttttatt agtagtttga
    29101 tcttgtgtcg tctcagctgc ttgaacttga tgtgcagtga ctgctgaacc tacaagcgtt
    29161 aatgcaacca ttgatggtag tttgtaatta aattttttcg ccattctatt tattactcct
    29221 aacatttatt aattattaat ctcattatag tacctgtttt atacacctgt gcgtatttaa
    29281 ccaatttgta atatactctt aatttatatt ttcactgtaa aaaaatgaaa aaaattttga
    29341 atctcaatgg tgacgtgaca aatcaaatag aattgatgtc aatttaagaa ttggtttatt
    29401 acaaatgttt ctataataga tacaactttt tttaattaag cgaggtcaac ttttttgtca
    29461 tttcaatatg ctcgatgcct tcctcaagaa atatattacc tctcatttta aaatttaaac
    29521 tttcatagaa tgggattgca tgacattggg cattcatggt agctacgtaa aaaccttcat
    29581 ctttagctaa tgattctaca gcttgcataa gcattttacc catgccttgt ccgcgatgtg
    29641 attttatcac agccactcgt tctattttga cagttgtttc attgatagga cgtatacgag
    29701 ctgtggcaac tggctgtcca ttatcatatc caatgaggtg aatagattca gattcatatt
    29761 catcaatttc actttcctct gggacgcctt gttcttctac aaacactttc tttcttatat
    29821 aaaagcaatc ttctaacatc tctggatcaa tcacttttga aaacataaaa tccctccata
    29881 tcctaacaaa atcatttaca tttaatttac taataataac atagaataac gattgttatt
    29941 ttccacaaaa ataattagac ataaaaaagc tataaagatg attcatagga accatcttta
    30001 tagctagata gagtgtattt gttataatgc ttcttttcta ataattgctg tatattgcca
    30061 aaagattcgc atttgagcaa tattccaatt attcattccc attgtaaacc cagaaggttt
    30121 aaagatattt acatctgtca cttctaaagc tgctgcgact aaatattgta atggaatact
    30181 aagtgatttc attgtttcag taatgccatt ttcatcatat acccatgaaa atggtaactc
    30241 tgattcgaaa acatcaacac tattgaaaat ttcaggtagg gctaaaatat aacatgcacc
    30301 atctacaata ggattctcga tactattttt gtaataaatt aggagtgctt catagttttc
    30361 tcgatgctca tgattgacgt aaaaaaattc acttctaaaa tgagccatat cttggctatg
    30421 aataatttgt tgttctaaca tattaaacat cccatttata tttgcaatct tttcgtatgt
    30481 ttttctactc atgtatgatg ctcctttcat aaatagtaaa tgttaatcgt catataatat
    30541 tagtttacaa caccattttg gttatttgaa gcttgtggcg tttgttgagt gccaccttga
    30601 ttttgattta agttttgatc tgtagcaggt tgttgttgat tattgacgct attgttgtta
    30661 ttactattat tactgtcatc agtattattc gaagatttat ctttatcagt ggtatcgttt
    30721 tgttcttttc ttaataaact actatctaaa ggtgttaaag gtatgagaga accataatga
    30781 ttaatgacac gttgatctaa gaaatcattt ttatcattaa taggtgataa ttctaagtct
    30841 ttacgaagta agtttgcata tttttgaatg ctttctacac ttggatgata atagtaaata
    30901 ccatttaaca tatcatcttt accttttaat tgcgcagttt taatttcaac atcatttgtt
    30961 aagtacattt ttgctaaagc tttaatttca gagttagtta aattatgctt tgcattttta
    31021 cctacaattt gaatcacgtt atcaagttta tcaatagaat caacttcctg tgctttttgg
    31081 aataaaatct taattaattc catttgacgt tgtccacgtt tcaagtctga atcatgatgt
    31141 ctagttctag caactgctaa agcctcatca ccatttaatt tttggtaccc ttttttaatt
    31201 ttaatcttac cagtatcatc tgtgtttggc tcatttaaat catatggtac atcatagtat
    31261 atgccaccaa gttcatttac agcttctaca aaggccttca tattaacgcg tacatagtaa
    31321 tcaacaggaa cattcatcgt tgcttcaaca gagtccattg ctgcaattgg accaccatat
    31381 gcatgtgcat gggtaatctt atcgtaatag ccgactttag gaatgtagct gattgtatca
    31441 cgaggaatac taagcattcg aatttggtgt ttagattgat taaaagtagt taaaatcata
    31501 gcgtctgatc tagagtgttc agcatcttgg cctttttttc ttcttccgtc attatcatcg
    31561 atacctaaga agagaataga gataggttgt tcttcaggat tgactttatt gtctcgtaaa
    31621 tttgattgac gattagcatt tttactatct tgagaagatt caaatgcatc ttgagacgtt
    31681 ttaaaaagta acgtagcgaa gacaattgga acaacaataa gaaccaatgc tagaaggatc
    31741 aaaaagtatt ttaaaaattt attcatgatt gatgctccta tattaaattt ttgttaaatc
    31801 ataatcataa ttttaaaata taattagttg aacgatattt atacaacttc attactaata
    31861 cattatagcc ttaaattgta gtactttata gtaaaaaata caaatgttat gtcaaagttc
    31921 cttgtaattt gaatgttttg ttaaaatcaa aaagcatttt aaaaaatata atgcgacttt
    31981 agtatgatgt cttaaaaaaa ttaaaggggt aatataaatt tgagtgattt atacaatgtg
    32041 ataaggtata attaaatttg ttgtaataag agcttatatg aagaaaatat tactaagtag
    32101 tataacgtat aagtgggtga cagatgaaat tgtaagttat attataacga agttaagttt
    32161 attgttttaa ttatttaatt tgaatacaat gagaggctat tagcgaatga aatttaataa
    32221 agagaaattg gttatacatg cgtgcgtgct tttgttcatc attatctcca taggattagt
    32281 ttttcatcga ttacaaacga aaacgaactc tatagaacct atacataaag aaacaaaatt
    32341 atcggataat gcgaaatatt tggttgatcg taataaaggc aaaggtgagc catcgaaatt
    32401 aaaggaagta tacaatagta aagatccaaa gtataagaag attgaccggt atttacaaaa
    32461 ttcattattt aatggttctg tagcggtata tgaaaatggc aaattgaaaa tgagtaaggg
    32521 ctatggatat caagattttg aaaaaggaat taaaaataca ccaaatacga tgtttctaat
    32581 tggttcagct caaaaatttt caacaggatt actgttaaaa cagttagaag aagaacataa
    32641 aattaatatt aatgatccag taagcaaata tattccatgg tttaaaacat ctaagcctat
    32701 tcctttaaaa gacttgatgc tgcatcaaag tggattatat aaatataaat cttcaaaaga
    32761 ctataagaac ttagatcaag cagtgagagc aattcaaaaa cgtggcattg atcctaagaa
    32821 atacaaaaag catatgtata atgatggaaa ttatttagtg ctagctaaag ttatcgaaga
    32881 agttaccggc aaatcatatg ccgagaatta ctatactaaa ataggtgatc cattaaaact
    32941 tcagcattcg gcattttatg atgaaaaatc atttagaaaa tattttgcaa aaggttattc
    33001 ttataatagt acaggacttt ctttcttgaa gccaaatgtt ttagaacaat attatggtgc
    33061 aggtaatata tacatgacac caacagatat gggtaaatta attactcaga tacaacaata
    33121 taaattattt agtcctaaaa taaccaatcc attattacat gagtttggta cgaaacagta
    33181 tccagatgaa tatcgatatg gtttctatgt taagccaaca ttgaatagac ttaacggggg
    33241 actcttcgga caagtcttta ctgtttacta taatgataag tatgtagttg tgcttgcatt
    33301 aaatgtaaaa ggaaacaatg aagttcgaat caaacatatt tataatgata ttttaaaaca
    33361 aaataaacct tataatacga agggtgttat tgttcaataa ttaatataga agatataact
    33421 tgtatatggc attaaggcat tgaccttatt tgactagtat acgagttata tcttcttttt
    33481 tatagaggtg aaaagtttaa aggataaggt tgaagtagta gggtgataaa tttgcgttaa
    33541 gttaaaaaga tataaacaat tgatgtgact gtcaatataa aaatgtacat aaatgattgt
    33601 ataagaatgt attaattgaa tggataataa tgattgtctt attagatata gaaggggact
    33661 taaccattta aaaattgaaa gataataaaa accagctaac aaaagtgtta gctggttaaa
    33721 ctttacatta ttaaaatgtg gtttataagt gtgaagagtg accgccttgc ataacccaat
    33781 atgttccgac aacgaaacaa agtgtgatta caagagcaaa gataactttg aatgtttgta
    33841 aacgtccatc tttaccttca gttaaatgca tgaacattaa taattgaagt ccagcttgga
    33901 tgaatgcaaa gccaaagata attgtcaact tcgcgtggaa tgttaatgac gtgtatagtg
    33961 ttacgtaaac tgctaaaagc gttaatacga tagatgcgat aaatcctaca gtatgtttca
    34021 ttattgtact catccgctat acaccatccc tatcatatat acggcagtaa agatgaaaac
    34081 ccaaacaaca tctaagaagt gccagtataa acttactata aataattttg gcgcattata
    34141 tttgtctaat ccgcgtcgtt ggatttggat taataaacaa atggcccaaa cgatacctag
    34201 cgatacgtga caaccatgcg ttcctaatag gataaagaaa ctagaccagt aagaaccaat
    34261 tgttgggtta acgccttctg atgcatagtg tgcgaattca taaatttcga atccaacaaa
    34321 gactaaacct aaaagtaacg taatgatcat ccaaaacatc attaactttt gtttttcttg
    34381 gcgcatgtag taaatagcaa taccacatgt gtaagaactg aataataatg caaacgtcat
    34441 tattaaaaca agaggcaatt caaataactc agtagtcatt ttacctgcat aatcgccacc
    34501 atgttgcaaa gttaatagtg tcgcaaatag ggtaccgaat aacgcaaatt cggctgtaat
    34561 gaaaatccaa aagccaagct tatttaattc gccttcatgt gtgcgtgaat caatagtgtt
    34621 tgtatcatga ctcatgactt acagcctccc tttctttaat acgcgcttct cttaatctag
    34681 cttcagtttc agcaacttca gcagcaggga tatgatatcc gtgatcgatt tggaaactgc
    34741 gataaatcat agtaccaaaa ataccgaata aacaaattaa tgctggaatt acagtttcga
    34801 aaattaagaa gaaaccgccg atagtcataa agataccaat ccagaatcca acaggagtat
    34861 tgtttggcat atgaatgtct ttgtaattat ggttgtctaa ataatgacga ccatgttctt
    34921 tcatatcaac aaatgtgtcg tagccattcc aatctggtgt aatggcaaag ttgtatttag
    34981 gtggaattgc tgatgcagta gtccactcta gagtacgacc aaggccatcc cagttatctc
    35041 cagttgcttc acgtggagat ttgaagtgac tgtatacgat actaacaaca aggaataaga
    35101 atccgattgc cattaataat gcaccgatag ttgaaattaa gtttaataag aaccaaccat
    35161 ctgatggcat ataagtgtat aaacgacgtg gcataccatc taatccaaga atgaattgtg
    35221 gtaagaaaca aacgttaaat ccgatcatga agaaccagaa gcaccatttg tttaatgttt
    35281 cgtttaattt gtaacccatc atctttggat accagaagat taaaccagct aagcaggcaa
    35341 atacaacacc agtaaccaat gtatagtgga agtgagctac taagaagtac gtattgtgat
    35401 attgatagtc agcagatgcc attgctaaca ttacacccgt aacaccacct aaaaggaagt
    35461 tagggatgaa tgctaatgag aataacattg gtgactcaaa tgtaatacgt cctttatata
    35521 atgttaataa ccagttaaac aatttcacac cagttggaat accaatcagc attgttgaaa
    35581 ttgagaagaa tgagttgatt aacgcaccat tacccattgt gaagaaatgg tgaacccaaa
    35641 ctaagaaact aaggaaagcg ataccggcag ttgcccatac catactttga tgtccgaata
    35701 aacgcttacg agcgaatgtc ggaataattt ctgagtaaat accaaatgct ggaaggataa
    35761 cgatataaac ttcagggtgc ccccataccc agaagaagtt agcccaaagc attggcatac
    35821 caccatgtgc aactgtgaag aatgctgtgt caaatattct atcagttgtc attaatgcta
    35881 acgctactgt taaaggaggg aaagcaagaa taacaattaa tgtagtaata aatgttgtta
    35941 ctgtaaacat tggcatttgc ataaacttca tagttggtgt tttacatctt aaaattgtta
    36001 caaagaagtt gatacctgta gctaaggtac caagccctga aatttgtata gctattaagt
    36061 aatagttaac acccggacca ggactgaatt cacctgctag tggcgcatag tttgtccaac
    36121 cagctgctgg tgaaccacca ataataaatg acaggttgaa taaaatcata cctgcaaaga
    36181 atagccagaa acttacgttg tttaatactg ggaatgcaac atcacgtgct ccaatttgta
    36241 atggaacaac gatattccat aaaccaaaga taaatggcat tgccatgaag ataatcatga
    36301 ttacaccatg tgtactaaaa atttcgttat agtggttaga ttctaaaaat ttgttatcag
    36361 gtactgttag ttgcgcacga ataagtaacg catcaatacc accacggacg aacattaata
    36421 cggcacagat taaatacata ataccgattt tcttatggtc tacagatgtg aaccattctt
    36481 tgtaaagata tttccataat ttaaagtaag taattactgc gattaaacca ataactaaga
    36541 atggggcacc aatttgtgcc attgtaatca tccagttacc tttaactagt aattgatccc
    36601 atggaaaatt cattaatgtc cacctccatg atcatcattg tcttgatctt gcgcatcttt
    36661 tgaaattttc ttcatttctt tcgcattttt cgattcatct ttcttgaact cattgttata
    36721 tggttcgtca tttccaagaa tcatcaattt cataccatgt cgtttatagt tcgcatttgt
    36781 aatttgagct ttacgagcag gtattaatgg tttgtctgat acatctttaa acatattttc
    36841 ttcactagtg aagtttggat ctttcaattc gaaattgaaa cgtttatatg catagaagat
    36901 gtattctgga tcggctgctg gatcaacaaa cgccatatgt gtaccattaa attctaaagc
    36961 tttattaggt gtgcttggta ataattgttt atcaaatgta tcttgatcta acgttttctt
    37021 acctttaact tctttcaccc atttgtcgta gtctttttga ctaacggcat ttactttaaa
    37081 tgtttgacgc gagaatcctt caccattgaa gttagaatta cgacctctga acgtaccagt
    37141 ttgagatgct tctaacgtcc aattcattgt catgccagtc atggcatatt tttgaccacc
    37201 taattgtgga atccagaaac ttgtcattgt atccatagct tgaagcttaa atacaacagg
    37261 acgatcttta gggattgtta atgtattaac agtctctata tgttcatctg gataagcaaa
    37321 gaaccatttg tatcctgcac ttactgcata tacaaccatt ggatctttct cactcttcgg
    37381 tggtttttcg taatcgtata aagttttaac tgtaggaata gctaaagcag caacgattat
    37441 gataggtatt acaaaccata ttgtttcaat gatggcatta tggtgcatct taccagattc
    37501 ggcattctta ttataactat acttgtaaat aaaaatggcg aacatgccaa gtacaacgaa
    37561 acaaataaca agcatgaaga cgattgaata aagaatcaag aacttctgac tacttgctac
    37621 tggccctttt gcgttgaaaa tttctatatt tgaacaacca ctaagtaaaa ttagtgtgcc
    37681 aaataataga agcaaagact taaattttga cacttttttg acctcctaat actacaaatg
    37741 tagggcttaa cattaatttt aagttattac acaatattta caagggctta tgggaaaaaa
    37801 attaataaaa ttgtatcaaa aatgttgata aatcaaggtg tgacgtgggt tcacacattt
    37861 gttaaaatta tgtgtacatt ttgtgactaa tagcgttttt gaaaaaattg cttcatattt
    37921 aaaagggaag ttgagaatag ctttcaatta aaggttaatt gaatgaaaat aaaaaaagtt
    37981 tgaaattgtc taataaattg aaaatgatgg ctagttcatt gaaaggaagc gcaaacattt
    38041 taatgaggaa tttgccaaaa gtaatttatc gttaaacaat gaaaagagga aatttgtttg
    38101 ttcaaaatca aaacattctt ttaaaaagag cagttaagtt ttaataattt gataggtagt
    38161 cctttggtat acagaaaaaa tacaatattg gggttaaagt tgcagatgaa gttggaataa
    38221 caaataaaat ggtagcaaaa catgtacgtt tgctaccatt ttatttgcag tatagtgatg
    38281 agggctattt aacctcaata tacctagtgg aaattttaat tgcaccatct gaatcagtga
    38341 catgatattt aatttgatat ttaccagatt tagatgtatc aatttggcca tcgactttaa
    38401 ttttatcggt taaatctcca tcttctttat caaatgcact tatgccgttt agaaggttat
    38461 aatcttgacc tttcttaata acgatatcat tagcgccttt aatttgtggt gtagtattcg
    38521 tcgttgcatc tgcatttaaa tttggtgtta ctagtgtagc agaaacaccg agggctgata
    38581 atgactgtaa tagtttattc ataatgtaac ctccgaatga taagtcattt ctgagttagc
    38641 tttactatac cttaatttat attatttaca catcatacta tagtcctaat cttaccctat
    38701 aaagaatgaa atttaaatat aaatttataa ataatgagga aataattatt attttgaaaa
    38761 tctgccacaa ttgtaaaatt aataattcag ggataatttg aaatcaaata taactttatt
    38821 cgacagtttg aacttaaatt gttgtgcatc ataacaataa gaaaagggag gcaaaaataa
    38881 taaagaacca ctaatgattt attatgtagt agtattttat cattagtcac agcttctgtg
    38941 ttggggactc gctgacttgc attgtctgta g
//
LOCUS       DAMZPP010000005        38348 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMZPP010000005 DAMZPP010000000
VERSION     DAMZPP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 38348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38348
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.005"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..270
                     /locus_tag="POH73_000190"
     CDS             <1..270
                     /locus_tag="POH73_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase"
                     /protein_id="HDI6886179.1"
                     /translation="DGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKGQLFKLLE
                     FSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            277..552
                     /locus_tag="POH73_000191"
     CDS             277..552
                     /locus_tag="POH73_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDI6886180.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            830..2047
                     /locus_tag="POH73_000192"
     CDS             830..2047
                     /locus_tag="POH73_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDI6886181.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIVFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDTNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(2160..2789)
                     /gene="vraR"
                     /locus_tag="POH73_000193"
     CDS             complement(2160..2789)
                     /gene="vraR"
                     /locus_tag="POH73_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HDI6886182.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(2779..3822)
                     /locus_tag="POH73_000194"
     CDS             complement(2779..3822)
                     /locus_tag="POH73_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI6886183.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            complement(3819..4520)
                     /locus_tag="POH73_000195"
     CDS             complement(3819..4520)
                     /locus_tag="POH73_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDI6886184.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            complement(4535..4921)
                     /locus_tag="POH73_000196"
     CDS             complement(4535..4921)
                     /locus_tag="POH73_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886185.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMHYLITK
                     KEQLSKLIDSLMLTLENKYIDIVEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            complement(5138..5896)
                     /gene="map"
                     /locus_tag="POH73_000197"
     CDS             complement(5138..5896)
                     /gene="map"
                     /locus_tag="POH73_000197"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDI6886186.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            complement(6101..6274)
                     /locus_tag="POH73_000198"
     CDS             complement(6101..6274)
                     /locus_tag="POH73_000198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886187.1"
                     /translation="MCRLAGPQHREIGSTISTGNASWGVGPNTENFEKRNSTGNAGWG
                     VGPNTEKLEHQFL"
     gene            6596..7582
                     /locus_tag="POH73_000199"
     CDS             6596..7582
                     /locus_tag="POH73_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDI6886188.1"
                     /translation="MNGQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQEIILSRLQSDKSEKAYKNIFNLNNQVEMLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            complement(7771..8184)
                     /locus_tag="POH73_000200"
     CDS             complement(7771..8184)
                     /locus_tag="POH73_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886189.1"
                     /translation="MKPTKVILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEIKFEFALFDSDMDNLKYIEYDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMVTESGVSLRINVTKSGFTKFINELVSLH"
     gene            complement(8213..8324)
                     /locus_tag="POH73_000201"
                     /pseudo
     CDS             complement(8213..8324)
                     /locus_tag="POH73_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8410..9141)
                     /locus_tag="POH73_000202"
     CDS             complement(8410..9141)
                     /locus_tag="POH73_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDI6886190.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHNFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEKQAKQVLIDRANRQKKSR"
     gene            complement(9143..10456)
                     /gene="murT"
                     /locus_tag="POH73_000203"
     CDS             complement(9143..10456)
                     /gene="murT"
                     /locus_tag="POH73_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HDI6886191.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNGDDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISRFDVAPFLHLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQAIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            10748..11248
                     /gene="ftnA"
                     /locus_tag="POH73_000204"
     CDS             10748..11248
                     /gene="ftnA"
                     /locus_tag="POH73_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HDI6886192.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEEAMFETHINYLTRIGDDSNALYLYEKELGT
                     RTFDEE"
     gene            complement(11330..11422)
                     /locus_tag="POH73_000205"
     CDS             complement(11330..11422)
                     /locus_tag="POH73_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886193.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            11640..12194
                     /locus_tag="POH73_000206"
     CDS             11640..12194
                     /locus_tag="POH73_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDI6886194.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKVHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            complement(12261..13331)
                     /gene="dinB"
                     /locus_tag="POH73_000207"
     CDS             complement(12261..13331)
                     /gene="dinB"
                     /locus_tag="POH73_000207"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDI6886195.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYRNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSTRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(13578..14108)
                     /locus_tag="POH73_000208"
     CDS             complement(13578..14108)
                     /locus_tag="POH73_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDI6886196.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSAGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            complement(14278..15639)
                     /gene="rlmD"
                     /locus_tag="POH73_000209"
     CDS             complement(14278..15639)
                     /gene="rlmD"
                     /locus_tag="POH73_000209"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDI6886197.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDMG
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMVIFVTN
                     GKNWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPHAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPSTQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(15720..16667)
                     /locus_tag="POH73_000210"
     CDS             complement(15720..16667)
                     /locus_tag="POH73_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDI6886198.1"
                     /translation="MRKRARIIYNPTSGKEQFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
     gene            complement(17305..17451)
                     /locus_tag="POH73_000211"
     CDS             complement(17305..17451)
                     /locus_tag="POH73_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886199.1"
                     /translation="MVADLDAIEGLFISIEVILLLTLFIVHVNGKNINFINALALIIL
                     RQTV"
     gene            complement(17490..18917)
                     /gene="gatB"
                     /locus_tag="POH73_000212"
     CDS             complement(17490..18917)
                     /gene="gatB"
                     /locus_tag="POH73_000212"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDI6886200.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLSGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            complement(18930..20387)
                     /gene="gatA"
                     /locus_tag="POH73_000213"
     CDS             complement(18930..20387)
                     /gene="gatA"
                     /locus_tag="POH73_000213"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDI6886201.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADANDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            complement(20389..20691)
                     /gene="gatC"
                     /locus_tag="POH73_000214"
     CDS             complement(20389..20691)
                     /gene="gatC"
                     /locus_tag="POH73_000214"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDI6886202.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            21058..22596
                     /gene="putP"
                     /locus_tag="POH73_000215"
     CDS             21058..22596
                     /gene="putP"
                     /locus_tag="POH73_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDI6886203.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLVRGEEKAKTHQKEFVMVGRLSVLAV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            complement(22685..23884)
                     /locus_tag="POH73_000216"
     CDS             complement(22685..23884)
                     /locus_tag="POH73_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HDI6886204.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGPTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(23897..25900)
                     /gene="ligA"
                     /locus_tag="POH73_000217"
     CDS             complement(23897..25900)
                     /gene="ligA"
                     /locus_tag="POH73_000217"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDI6886205.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEFKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVSDIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            complement(25904..28096)
                     /gene="pcrA"
                     /locus_tag="POH73_000218"
     CDS             complement(25904..28096)
                     /gene="pcrA"
                     /locus_tag="POH73_000218"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDI6886206.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVSSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            complement(28093..28785)
                     /locus_tag="POH73_000219"
     CDS             complement(28093..28785)
                     /locus_tag="POH73_000219"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HDI6886207.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVAFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKVAKHTKANTDLTTEDLEAYAQMVNHLYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            complement(28968..29270)
                     /locus_tag="POH73_000220"
     CDS             complement(28968..29270)
                     /locus_tag="POH73_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886208.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            complement(29379..30674)
                     /gene="purB"
                     /locus_tag="POH73_000221"
     CDS             complement(29379..30674)
                     /gene="purB"
                     /locus_tag="POH73_000221"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDI6886209.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            31524..32690
                     /gene="scpA"
                     /locus_tag="POH73_000222"
     CDS             31524..32690
                     /gene="scpA"
                     /locus_tag="POH73_000222"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HDI6886210.1"
                     /translation="MKRNFPKLIALSLIFSLSITPIANAESNSNIKAKDKRHVQVNVE
                     DKSVPTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVL
                     NTEGNIDYIVTISPKVTKDSSSSSKYTINVSSFLSKALNEYKDQQITILTNSKGYYVV
                     TQNHKAKLVLKTPRLEDKKAKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQ
                     YVNKLENFKIRETQGNNGWCAGYTMSALLNATYNTNKYHAEAVMRFLHPNLQGQQFQF
                     TGLTPREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAILGSRVESRNGMHAGH
                     AMAVVGNAKLNNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYQWYSSIYGY"
     gene            32721..33044
                     /locus_tag="POH73_000223"
     CDS             32721..33044
                     /locus_tag="POH73_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HDI6886211.1"
                     /translation="MEQFELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKHK"
     gene            complement(33338..33511)
                     /locus_tag="POH73_000224"
     CDS             complement(33338..33511)
                     /locus_tag="POH73_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HDI6886212.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIIFVGKKIQ"
     gene            complement(33492..34094)
                     /locus_tag="POH73_000225"
     CDS             complement(33492..34094)
                     /locus_tag="POH73_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDI6886213.1"
                     /translation="MSFVTENPWLMVLTIFIINVCYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            complement(34364..35185)
                     /gene="nadE"
                     /locus_tag="POH73_000226"
     CDS             complement(34364..35185)
                     /gene="nadE"
                     /locus_tag="POH73_000226"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDI6886214.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIAELKQFIKSYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALQFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKQLYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            complement(35178..36647)
                     /locus_tag="POH73_000227"
     CDS             complement(35178..36647)
                     /locus_tag="POH73_000227"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDI6886215.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVYTYKMKFIKSFEAIDLHHNIYENGKLVYQIPTEDESRDYLAQGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            36831..37907
                     /locus_tag="POH73_000228"
     CDS             36831..37907
                     /locus_tag="POH73_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HDI6886216.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSINYHIAQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKYYEYPTSLIKEVPIEHDHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKKSNANQCPFHH"
     gene            37927..>38348
                     /locus_tag="POH73_000229"
     CDS             37927..>38348
                     /locus_tag="POH73_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDI6886217.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNLFEVIKSVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGSNTISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAA"
BASE COUNT        12045 a         6894 c         5560 g        13849 t
ORIGIN      
        1 gatggcatga ttagtgaatt attcgaagcg acagatgatt ttgattacat tgtggctatg
       61 gatcaaagta acgttgataa tattaaatct atcaatccta atcttaaggg acaattgttc
      121 aaactgttag aatttagtaa tatggaagag agtgatgtac cagatccata ctacacgaat
      181 aattttgaag gtgtatacga catggtatta tcatcttgtg ataatttaat agactacatc
      241 gtaaaagatg caaatttgaa agaggggtag ttttttatgg aaaataaatt tgttcctggt
      301 attttaattg gtgccgtaat tggtggtgca attagtttag ctgataaatc tacacgtcaa
      361 gctttagttc aatcagttaa agatgcaaaa aatggtaacc gcactcgtaa gccttctaaa
      421 gtcagcaaga ttaaagacga agttttatat tggaaagatg ttgttgaaga aattcgtcgt
      481 aataatcctg aattagaacg ttcattaaag gatgcgaaag aaacatttgt taatagaaaa
      541 aatcaacgct aagatgattt tgtttttata aggtaatact taacaatttg gtgtacgcct
      601 ttcgtagtta tcattattaa atgataatac aatgattggc caaactgcat tgtttcagta
      661 ttgcctttgt tttaatttta aatcaaaata gcctatgaaa gatttaaatc aattaaattc
      721 tataatatta tcatttttaa agcatatcat tgtttagttt ttttataatt ggataaatac
      781 taatagttac tttataaaac attacataga gaaaggttaa ggagtgcaca tgtcgaaaaa
      841 ggaccactct tcttcaaaat accttaattc tgttaaggaa gcgcaagagg agtcaaaaaa
      901 gaaaaataaa agtaatccca aaattgatgt tgatcgtaca tatattgaac ctcaacaatt
      961 ccaatctaag aaacctaaaa aagatgatca ggttttcttc ttatcaagat taaataaacc
     1021 tgcaaaatat aagaaagact ctaatttctt atcatatctc atctatcgca taggaaaaga
     1081 tgatgcctca ggactagcag cgcaaatgac ttaccatttc gtacttgcta tgttccctat
     1141 gttgcttttc ctattaacat tattaccatt tttcaatatt aagcagagtc aaattactaa
     1201 tatgttaagt aatgcacccg ctgaaacatc tactctaatt aagagtgtaa ttggtgatat
     1261 aactcaaaac tccagtggtg gcttattatc tatcggtttg attttagcaa tttggtcagc
     1321 ttcaaatgga atgactgcaa ttatgaattc tttcaatgtt gcttacgatg tagaagatag
     1381 ccgtaatgga atcgtattaa aactactaag tgttgtcttc actgtagtta tgggcgttgt
     1441 gtttgtagtt gctctagcat taccaacgct tggttctgta attagtcatt tcctattcgg
     1501 tccacttgga tttgacgaac aagtgaaatg gatttttaat cttattagaa ttgtgttacc
     1561 aatcattatt atatttatcg tatttatcgt gttatattcg gttgcaccta acgttaaaac
     1621 gaaacttaag tcagtattac caggtgcagt atttacttca attatttggt tggctgggtc
     1681 atttggtttt ggttggtata tttcaaattt tggtaactat tctaaaacat atggcagtat
     1741 cgcaggtatc atcattttgt tactatggtt atatatcaca agttttatta taattgtcgg
     1801 tgctgaaatc aatgcaatca ttcatcagcg tagtgtaatt aaaggtaaaa cacctgaaga
     1861 agcagcatta gaacatgatg acaataataa aaatcattat aatgagaaca ctaggtacga
     1921 atatgatgaa gatactaatg caacacatca acgaacgtat catgttgacg aacatcctag
     1981 tgatacctat ccagaagatg ataaaaatat aaaagacaaa atcgttgata agcttaaaaa
     2041 agactaaata acaacaaaac agaggttttc gctaatgatt gcgaagacct ctgtttttta
     2101 gtgtatattt gatgttatat tgtgattggc gtaagtaact tttcttaatt cgataagaac
     2161 tattgaatta aattatgttg gaatgcatag ataacagctt gtgttctatc ttgcacttct
     2221 aacttactta aaatgttact cacatgtgtc ttaaccgttt taatagtaat atgcgatgca
     2281 ctagcaattt cttgatttga gtaacctttc gcaatcaata ataatatttc catttctcgt
     2341 tctgtaagca tttcatataa ctctgcgcgc tttttcatac ggttacgcat tttcactaaa
     2401 acttccggtt caaaaacaga ttctcctcta gaagttttac gaactgcatc ggcgatatct
     2461 tttgcacttg ttgtttttaa aatgtaacta tcgacacctg catctaatgc acgatatacc
     2521 tctttatctt caataaaact agttaacatt aatactttaa tttgcggtaa atctttttta
     2581 atctgagtcg tcgcttctac accatccatg tcttccataa gtaaatccat taatattaaa
     2641 tctggcttca actcatgggc tttggcaatt gcttctttac cagaagcgcc ttcaccaact
     2701 acttcaatat cactttgcgt tgatagataa cttgaaattc ctatacgtac catttcatga
     2761 tcatccacaa acaatacttt aatcgtcata cgaatcctcc ttatttaaag gtgctttcac
     2821 ctcgatacgt gtacctgaat ctggcaatga tacaatatgg aacgttgcac caatttccaa
     2881 agctctttca cgcatatttt taagtccata actttgttct aatttttcat caacattaaa
     2941 acctttacca ttgtcttgaa ttctcaacaa taaataatcg tctttattaa acaattctac
     3001 tgtcactttt gtaccgtttg aatgacgcaa tgtattcgaa attgcttcct gtgtaattct
     3061 gaacaaatga tcttcaatac ctttaggcac tttaaaatct tgtatttcat gcacaacttt
     3121 cattggcact tttttttgta aatcaataac taaatcttta ataccctcac ctaaagattt
     3181 gtcttttaaa ccaagcggtc ttaaatgtaa cagcaaagca cgcatttcta gctgcgaatc
     3241 ttgaaccatt ttctctaaaa tcggaatttg ttggtctaat ggtggttcta acttcgtttc
     3301 tttgatagca gaaagcatca tacttgccgc aaaaagttgc tgactaacag aatcgtgaag
     3361 ttctcgtgct agtctttgac gttcatcttc aataatcttt ttaactttca catcattaat
     3421 attataattt tcattggtta agttttgagt tttaagtcgc aacttatgca attcttgatt
     3481 taaaggtaca agtgtatggt ataagtctaa cgtttcacta tatatttcta tattttgatc
     3541 attaatgcca actgtttcgc cttccattga acgctcaatt tgcgtcttaa tccaatcatt
     3601 ttgctgattg attttgtaag cgagtaccga accaacaata atacacaata atatgatgat
     3661 gagatttaaa aataaaaaga ctggtattcc gaatatttgt gtataaaaca taccttgaaa
     3721 atagatgata tttacaaaaa ctttatcgat gaacagaaat gcagctagca tgctatatac
     3781 taagatgagc attgaaccaa ttgttctaat gtagtggttc atcgataaat cacctctacg
     3841 tctccgataa acgttgatac gtagatatta actgtatagt tatccggttt catcatttct
     3901 tcaatatgaa tattgttatt ttcaacttta tatgattttt cattcacgta agtacttcca
     3961 taaaaagcag ctacatgtaa attaatattg taattaaccg gcaatataac ctgcacttta
     4021 cctaaaatgt gtctaacaac aatggtatta ttttccttaa tatttgcagc ttttgttaag
     4081 tcaatatgta ggtcgccaat tccatgttga atttggacat cttcccactt atatacataa
     4141 actggtgtac gttgctcacc aaaccacttt tgtttaataa aagatggtga agtcataacc
     4201 tcatccgtcg caactacttt tttgggttta aacttgtgta ttaaataacg cacaataagt
     4261 agtaataaaa agacgaacaa gatgatgatt gtatacttat tagacaataa tgtgaatgca
     4321 attaatagcg cgcctatcca aaacgccaaa aggccacgta ttttatgaaa ataaagatat
     4381 cctacataaa ctaatacaca tcccaataat agaacgagta aaaagccaat tttttcaaaa
     4441 aatatgtagt aaaaattggc aataatcatt aatgcagtaa aaatgatcaa catttgcgtt
     4501 gatatatatt tgtgtgtcat gtttcgccgc ctttctatgc cacagcgttc atataattta
     4561 ttagatacga tgttttttct gttgctattt tttcagttga agtttgttga atttgagttt
     4621 caatttgtgc ataatatgct tcaactttat tcaactctga tttaatattt tcaatttctt
     4681 gattattgat ttctcttgca cattgaattt gaaatgcttc aacaatatca atatatttat
     4741 tttctaatgt tagcattaga ctgtcaatta acttgctaag ttgttctttt ttagtaatta
     4801 aataatgcat ataatcatct aaatttttca ttttttcatc taacattaaa aggtagtgct
     4861 tgatattatt tcttactgtt gctctcaaaa actgttcaat ataacgttca acatagttca
     4921 taactatcac cttttataat aagttttaaa gtaccaaatg cgctatgtat cttcgttgat
     4981 gtcgatgata tgtttgctta taaactactt tattacaaca tatttttgaa atgacgcatt
     5041 gattgtgttc ttaattatta taaaacagtt ccgaaaccaa tatgaatagg ctccggaact
     5101 aaatgatgtt catactttag tcttagtata tgttgaacta ttcttcttca atctttgtcg
     5161 ttaaaatcgg accatcctta gtcacgataa ctgtatgctc aatttgagca acaaaacttt
     5221 tatcgctcgt ttcaaaagcc cattcatttt taccttctgt aacgaatgat gcatttgatg
     5281 agataaacgg ttcaatagct aataccatac cttcagttaa taatgttttg tcttttggat
     5341 caaagtaatt aagtacatgt gctggcgctt catgtaatga taaaccaaca ccatgacctg
     5401 ttaagttttt aatgactttc aaatcatttt gtctagctgt attatgcacc gctttaccaa
     5461 tgttactcaa cttagtaccc ggttttactt ttgcaattgc attctcaaat gccatcgttg
     5521 ctacgtcaca tactttttgt ttcattggat cgtctgattc tccaacgaca aatgaaatgc
     5581 ctgtatctgc ataatagcca ttcttcaatg ccgatacatc aatatttact aaatctcctt
     5641 cacgaatgac acgcttactt ggaatcccat gtgccacctc ttcattaaca ctaatacacg
     5701 tttgaccagg aaaattttca tcatgaattg gcgctgaaat agcgccgtat tcttcaaata
     5761 actctttcgc aatattatca agctctttcg tagtgatacc tggtttggtt gcagcttgca
     5821 ttgtattgcg cactttagcg catatgtatc caatttcttt taacgcttgt aattcttctt
     5881 ctgtttttac aatcatttta tcccgttcct tttttatata attataactt attatagcaa
     5941 aaattctttg atatactaaa gatgttactt tagtattttg gaaagtgagt gggacatagt
     6001 gtgataaaga aaattatttc ttaatcgtcc ccaacttgca ttgcctgtag aatttctttt
     6061 ttgaaattct ctgtgttggg gccccgccaa cttgcattgc ctatagaaat tggtgttcca
     6121 atttctctgt gttggggcct acaccccaac ctgcattgcc tgtagaattt cttttttcga
     6181 aattctctgt gttggggccc acaccccaac ttgcattgcc tgtagaaatt gtagatccaa
     6241 tttctctatg ttggggcccc gccaacctgc acattatcgt aagctgactt ttcgtcagct
     6301 tctgtgttgg ggcccctccc caacttgcat tgtctgcaga aattggtgtt ccaatttctc
     6361 tatgttgggg ccccggacta gaattgaaaa atgcttgtta caagtgcatt ttatttcagt
     6421 caactactaa caatataaca ttgtggagcc cagagctttg attaatgtat atgaaagtca
     6481 aagtaatgca taagcatgat tatttcaaat gttttacata catgaacttt tccaatgtac
     6541 gatactatta ttataaagcg ctcgctaaga ttttacgatg attagaggtt aaaaaatgaa
     6601 cggtcaatgg tataaacatt taattggtgc cagaacaatt aaaactggta ttgccatttt
     6661 tttaacggct gtcttttgta tggcactaga tttaacaccc atctatgcca ttttaacagc
     6721 tgtagtcaca attgaaccaa ctgccaaggc atcacttata aaaggttatc gtagattacc
     6781 tgctacggta attggtgcag gctttgcagt attatttaca tatttattcg gcgatcaatc
     6841 accttttaca tatgcattga gtgcgacgtt cacgatttta ttctgtacaa aactcaaatt
     6901 gcaagttggt acgaatgttg ctgtactaac atcattagct atgattccag gtattcatga
     6961 tgcctacata ttcaactttt tgtctcgaac attgacagcg attattggac tcgtgacatc
     7021 aggtttaatt aacttcatgg tgtttccacc taagtattat ggtcaggtcg aagaaaaatt
     7081 aagtaagaca gatgccttga tgtataaatt attttacaat cgctgccaag aaattatctt
     7141 atcaagactg caatctgata aaagcgaaaa agcatataaa aatattttca atttaaacaa
     7201 tcaagttgaa atgttaataa gttatcaacg tgatgaatta agctatcata agaaaaaaga
     7261 atgtgattgg aaattgctca atcaattaac taaacgtgca tatacgaatc gtttgtttat
     7321 cactcattta tcaaatatca tttacttgcc taaaaatact cgtgttaact tttcaggtga
     7381 cgaaaaaatg gcattgctaa aaattagtag tagcatcaaa gatatttttt atgatggttc
     7441 atttaagaga gaagatgatt ctgtagaaac tttacgttcg acaataaaag cacttgaaat
     7501 tagtggtgaa aatcaaatta aaagtcatat actgtatgaa gttttaatga tttatcgact
     7561 gctagacagc agatatgcat aatttaagtg ccttatgcta attggaatca ttagatatat
     7621 tcttacttta tcagtaattt tatcgtatat agcatgtccc tttttaaaat tacaagcata
     7681 tatattcaga ctcaaatata gccacacgcc taaactacgg agggatgtgg ctgtcttttt
     7741 tgattgtaat tgtgtatcag atttaaatca ttaatgtaac gagactaatt cattaataaa
     7801 tttagtaaaa cctgatttag taacgtttat ccttaacgaa acaccagatt ctgttaccat
     7861 gtttgaatgc cttaaaccag aatcaaaatc aatataaata caaagcagtt cttgattaaa
     7921 atcaataatc gtaaaatgta aatcaggttc tataaaatat atttcggtaa ctggattgtc
     7981 gtattcaata tattttaaat tgtccatgtc actatcaaaa agtgcaaatt caaatttaat
     8041 ttcttgatct tcgaaagttg cactagtgtc gaaataataa attttattat cttcttcgat
     8101 aactacatct tttaaaataa atgttatcga tgttttgcta tgtaaataag atgcatcttt
     8161 taatatcact ttagtaggct tcatcgtcaa attcctttca tcattcattt tttcatgttg
     8221 cataatcacc attactattg ataattctag gatagatact cattgtataa taattattca
     8281 ctagaacaaa aaaaacacac atcaatttgg ttgtttccat ccaaaataat gtgtgtgttt
     8341 atacatatat gattaattac taaacttcat cgtttatcaa ttttaaaccc attgatgcga
     8401 tgttcagagt taacgagact tcttctgtct atttgctctg tctattaata cttgtttcgc
     8461 ttgcttttcc gcttcattat ctatttcttt aggctcaaac ggaataccct tacgttcaca
     8521 agctttttct aacagataat cagttatttc gtaattttta ggtaaaattg gtccgtgtaa
     8581 ataagtacct aataaatttt tataatgaat gccttctttt ttatcttcat cattattacc
     8641 ataaccaaaa gtaacatgac caagtgtacc gaaattatga tatgttctac caccgtgatt
     8701 ttcaaaacct acaatagttc caaaagtatc actttcgata acaatatctc ctgttaatcg
     8761 gtttgtcttt gattcagtat aaaaatctaa aatacctaac ccttctaatt ctgtaccatc
     8821 aggcgtgata tatttttttc ctaaaaattg atagcctcca caaatcgtta atcccggcat
     8881 accatcttca atcgcttctt taagtggtgt cttaatttta cttagttctt ttgttgctaa
     8941 tgcttgttct ctatcacttc caccaccgat aaagaaaata tcacattcat caaaggtaat
     9001 accttctgtt tcattgattt ctacgacatt aactttaata tttcgttttt ttgcacgttg
     9061 tcttaaagca ataatatttc ctatatcact gtataaattc aatttatctg acataaaatg
     9121 ataaatagtc aattcatgca tattatgatt gacctccttc aaacgaacgg tttaattgtt
     9181 caagcatagg cgctaatgat gtatagtttg gtattgcaac tgtgaaacct ttataatcca
     9241 tagtctttgc cgttgcttta taaatatcac gctcaactat aattggtact tcaacctctg
     9301 ctaacttcaa tcgcaattga agttcttctg ctcgtgtacc tgtcacgatg atagcttcaa
     9361 tttgttgctt agataatttt tcgaaatctg catcataaat ccatgaagta tctcgaccat
     9421 ctgcagcgtt atcatttagc gaaataacat acactttttc gccttctaat tgttcaccaa
     9481 ctgataaact tgcattcatt cctgcagggt ttttagctaa attgatcatc gcttcttttc
     9541 gttctttttt aaagtactgc atacgaccat tgtctgatgt atacgtttca aagccatttt
     9601 taattgcttg ttcatttaac cctagctctc ttaaaactgt atatgctgct aacgcgttat
     9661 aagcgttaaa gtcacctgct attttcatat catatttttc atcattgata tttaaatgta
     9721 aaaacggtgc cacatcaaat cttgatattt catattttgc ttgctctcgt ttgaaaccac
     9781 actgacagtg ataatgacca atttgattat aatgaatata atcatattgc aataagcgac
     9841 cacagtttgg acaatatcta ctttcattca tcgtactttg ttcaaattca tgagcatgtg
     9901 ctttcatacc atagtacaca atcgtatcac ttgcgatttt caaacgactc acaaatggat
     9961 catcaccatt tagcaataat ttgatgcctt tattactaat tgtctctgca atgttattaa
    10021 ccataatatc aatttcaccg aaacgatcca tttgatctct aaagaaatta gtaaatacca
    10081 tcattgaagg tgtaacttct tttaacacac gtggaatcga accttcatca atttcgatta
    10141 ccgcaatttt agtcttaggt gttgattgca tgatgaatgc agaagttata cctgcagcca
    10201 tattagcacc ttcattattg tgtataattt gaatattatt cgcttttaaa gtatgtccaa
    10261 ttaagtttga agtcgttgtt ttaccatttg ttccactgat aaatacaata tcatcaactt
    10321 gctctgctaa ttttcttaat atatctgtat ccacttttct agcgatttgt ccaggtaaat
    10381 ctgttcctct tttacctact gctctacttg ctttacgcgc caatttcgct agatggattg
    10441 ccgtccactg tctcatgtgt ttcctcctca aatttccact cgcatcatta taacatgaca
    10501 aggcaacttc aaaaaagttt ctcaatcaca aattgataca gtgtttctaa attataatca
    10561 ttctcattta gataattaaa aatgatattt attctcaatt aaatctcggt aaatgataca
    10621 ttttctcaat tagagtgcta aaatcagtca ttaaattata attattatta tttacaattg
    10681 agaatgagtt tgttatacta agtttaattc atagtaattt taatttacaa ataagaggtg
    10741 tatcaaaatg ttaagtaaaa atttattaga agcattaaat gatcaaatga accatgagta
    10801 ctttgcagcg cacgcatata tggcaatggc agcatactgt gataaagaat cgtatgaagg
    10861 atttgcaaac ttcttcattc aacaagctaa agaagaacgt ttccatggac aaaagattta
    10921 taactatatt aacgacagag gtgcacatgc agaattcaga gcagtttcag caccaaaaat
    10981 tgacttttca agcatactag aaactttcaa agacagctta tctcaagaac aagaagtaac
    11041 aagacgtttc tataacttat ctgaaatcgc tcgtcaagat aaagattatg caactatctc
    11101 attcttaaac tggttcttag atgaacaagt cgaagaagaa gcaatgtttg aaactcacat
    11161 caattattta actcgtatcg gcgatgacag caatgcatta tatctttacg aaaaagaact
    11221 tggcactcgt acattcgacg aagaataatt aaacatcact acaatagaca gataaatatc
    11281 atacgacatg atagacattt gggtcactta caataaccca atgtctatat tattttgctt
    11341 tacggagatc actagattca ttttctgaat cattgatctg cgttttttca ttttcaaggc
    11401 taattattgt atttttagtc atttgttttt ttaaactact aatgttaata actctaaatt
    11461 taatgttgta ttaatttgac cgattttaaa gcatatcatc atttactttt taatcagagt
    11521 tacatccaaa tgatagattt cacgttatac cttcacgtat aatattatgt atcgtttgta
    11581 agcaaatgac taaaaagttt attaatatat acatttaatt aattgaaagg attgactaca
    11641 tgatacaaga tgcgtttgtt gcacttgatt ttgaaacagc aaatggtaaa cgtacaagta
    11701 tttgttctgt cggaatggtt aaagtcattg atagtcaaat aacagaaaca tttcatactc
    11761 ttgtgaatcc gcaagactat ttttcacaac aaaatattaa agttcatggc atacaaccag
    11821 aagatgttga aaatgcacct acgtttgact acgtatttcc atatatgatg caatttattg
    11881 cagatttacc tgttgtcgca cataacgcgg catttgatat gaacgtctta catcaaagca
    11941 ttcaaaatat tggtttacca actccaaatt taacttactt ttgtagttat caacttgcta
    12001 aaagaaccgt tgattcgtat cgatacggtt taaaacatat gatggagttt tatcaattag
    12061 attttcatgg tcatcatgat gcattgaatg atgccaaagc atgcgcaatg attactttta
    12121 ggctactgaa aaattatgaa aacttaacat atgtaactaa tatttatggt aaaaatctaa
    12181 aagataaagg ctaggactaa ataaaatact cccttcaaag taagcattgt aaaaatgtaa
    12241 actttgaagg gagctttatt ttatataaag tcatatatcg tcatattttt ataagttgat
    12301 tgttctaaat tacctacagt gacaccaata agtcgaattg gtacatcagg gtcttttaaa
    12361 tcgttataaa gtaaatatgc aatattataa atatcttctt cagaactaac agaatctctt
    12421 aaactcatct gtttagataa cgtttcaaat tgataagttt taattttaac cgttacagtt
    12481 ttagccgact tctgtaattt atttagacgt tcagctgttt tacctgacaa ctcccatact
    12541 tttcttaaaa tctcttcatc atcattcacg tctgttgcaa atgtgcgttc agtccctact
    12601 gattttctta ctcttgttga tttcacttca ctatggtcaa taccgcgtgc cttgttatat
    12661 aaaccccgac ctctttttcc aaacaaacgt attaattcaa attccgtttt ctcatataaa
    12721 tctctaccgt taaaaatacc attatcatgc attacttttt tggaagcttt acctacgcct
    12781 ggaaaatctc caatatccaa tgtcatcaaa atatcatgga catttcgata atcaatcaca
    12841 gtcataccat caggtttatt cataccactc gctaatttag ctaaaaattt gttataagaa
    12901 acacctgcag atgctgttaa atgtgtctgc tctagaatat cttttctaat atactgagca
    12961 attttcgaag caggaaggtc tggtctcact aattctgtaa tatctaaata cgcttcatct
    13021 aatgacatcg gttctacctt atctgtataa ctacggaaaa tagacataat ctgcgcagat
    13081 gtttctcggt aagcaccaaa attacttgtg acaaagtatc catttggaca taatttatgc
    13141 gcttgtgaca taggcattgc tgaatggacg ccgtattttc gtgcttcata agatgccgta
    13201 gagacaacgc ccctactact tgctttacca ccaacaatga ctggtttccc tttcaatttg
    13261 gggttatctc tcatttcgac ttgtgcaaaa aaatagtcca tatcgatatg aataattcgt
    13321 ctctcagtca agtgctcacc tccctactaa tttttacttt tataacgcac aaaaatatct
    13381 caacataatt atacgctgtg tacgattttt ttacataaat cttgcactta gcgataacta
    13441 tattgagata actacaagtt gttataaaat caattgctat ttaagcatga tgatgaagac
    13501 gattgagtaa gaaaacatag gtaatctgaa ataattcaag caaattcatt ttgctggtat
    13561 catcatatta aaatttatta ttgagtcggc ttttgatgat acaaataaat actatcttca
    13621 tcttgttgaa catatttaaa actactagat attgttaatg caacaagcag aatatctata
    13681 aagcaataag cggtatgcat actaaacata aaaataagtg atgaataacc aaatacctta
    13741 attaaaataa gcaagccagc acttaatagg attagtggtg acagcataat aattgagaat
    13801 tgccatttgt tgaagcaagc atctgctgtt tggaataaga ttctgtcttt ttttatatta
    13861 aacataggtt tgctatcttt tttaaataac agaaataatg ctctatggat aagttcatgt
    13921 aaaatcaata aaataatgaa tccagcaaac ccatatacaa gattgatgat gatattttga
    13981 tcgacaaccg ctgtgacacc taacgcccac ttatacgtaa ataaaatcac gaataacgca
    14041 ataacaagtt gcaagataat aaaccttcgc atttgaaaat tatttgtcgt taaatcaatt
    14101 ttatgcatta ccaaccctcc cgatcatgac attcttattc ttctttaaat atagtataca
    14161 atgtcacatt taatttaaaa agttcatatc aagaaagtaa attggctgta ataaaatttt
    14221 aatatacgac ttctttcttc acttattaag gcgaaatttt atctcaaatc atgtgcgcta
    14281 tttcaaattg aataatgcca ctgtctcaac atgtgttgtt tgtggaaaca tatctaccgg
    14341 tgttacctct tcaagttgat atttttcagc taataataat gcatcacgtt gctgtgttga
    14401 gggattacat gaaatataga caatacgctt aggttctaat gtaagcaaag tctgaataaa
    14461 cgtttcgtca cagccctttc ttggcggatc aaccattaca acatctggtt taatcccttg
    14521 tgctttccat tgtaaaataa cttcttcagc tttcccacag acaaaagttg tattattgca
    14581 ttggtttata gtcgcatttt gttgtgcgtc ttcaattgca gaaggtacaa cttcaacacc
    14641 gtatacatgt tttgcatgtg gtgccatata tagtcctatt gttccaatac cacaataggt
    14701 atctaataca acttcattac ctgtcaattg cgcatactca attgctttat tatataattt
    14761 ctctgtttgt tcagaattaa tttggtagaa tgactgatca cttattttaa atgtactatc
    14821 tgttaattga tcaataattg tatctttacc atatagcgtt atagactgac gtcccataat
    14881 aacattagag tggctatcat taatgttttg tttaatgctt gtcacattag gaaatgcatc
    14941 taatatcttc tcaacaacag catttttttg tggccaattt ttaccattag ttacaaaaat
    15001 aaccatcatt tcgtctgtat gatatcctgt tcttacaacc aaatgtctca ttaaaccttt
    15061 tttcaattgt tcttgataaa tacttacatt taaatctttt aaaatagatt taacttcatt
    15121 catcacttct tgatgttgtg aatcttgtat taaacaactt cccatgtcaa taatgtcatg
    15181 gcttctttga cgataaaagc ccataataac ttcattctgt tcattcttac caactggaat
    15241 ctgggacttg tttcgatatc tccaaggatc tgtcatgcca actgtatcgt taatcttaga
    15301 attatcaaaa tgcgcttttc gctgaaacaa attaatcact tgttcctttt tcatttcaag
    15361 ttgtgcttcg tatgataagt gttgaagttg gcacccacca caacgttcat aatatataca
    15421 tggcggtgca acacgattct cacttctcgt atttatatta agcaacttac ctatcgcaaa
    15481 gttctttttt actttgataa ttttatattc aatttgttca ttaattaaag cttgtggtat
    15541 gaaaatagga aagcgatcta tttttacgac accatgacct tcatgcgtta aatcaacaac
    15601 tgttcccgtt ttaatgtcat ttttagctat tgcttgcaaa attttacctc caaaatgaac
    15661 aggttaggaa caaaattatg cgcttcctaa cctgccatta tatatttcac tacttctgtt
    15721 tattcttcga ttaaattgtc atcaacatga tcattattta ttaactcttc atttacaata
    15781 tcatttggtg caaagacatc tatatgacgt tctagattca agaaattcgc tggtaattta
    15841 ccaccatatt ctccatctac atttagttgt aagtctgtga atgatgaaat attaattgcc
    15901 tttgcttttt cataaataac tttaggatgc ttagtatgtt ctcctcttga agctaaagtc
    15961 ataatatgac caagttctgc aaggtttgat ttttcaacta taattaacgt aaaatagccg
    16021 tcatctaact tagcgtccgg cactaatttt tcaaatcctg ccattgaatt tgttaaacct
    16081 aaaaagaata ataatgcttc tccttggaaa acattaccat catattcaat tcttaaatct
    16141 acagctttca tttgaggtaa catttcgaaa cctttgatgt aataagcaaa tggaccaaca
    16201 atagatttca atttactcgg tgtttcataa gagacttgcg tcaattgtcc gcctgcagct
    16261 aaattaataa agtatcgatt attcatttta ccaatatcta ctttagtaga atgaccttca
    16321 atgatgacat caagtgcccc catgatgtca ttaggtatat gcaatgcacg tccaaagtca
    16381 ttaacagtac ccataggaat gacacctagc ttaggacgat taggcttttc tgcgatacca
    16441 ttaactactt catttaatgt tccgtcacca cctgcagcga ttaatacatc ataattttca
    16501 tgcatagctc tttctgcttc aagtgtggca tcacctattt tctcggttgc atatgcactc
    16561 gtttcatatc ccgctttttc taattttatt aaggcatcag gtaattctct tttaaattgc
    16621 tctttacctg atgtcgggtt ataaatgatt ctagcacgtt tcctcatatc ttatccctct
    16681 acttaaaatt catatatttt aacttcatct tggtttcgtc taataggagt gggacagaaa
    16741 taatatttaa caaaatttat ttcgttctac cccaacttac attgtctgta gaatttcttt
    16801 tcaaaattct ctatgtttgg ggccccaccc caacttgcac attattgtaa gctgacagaa
    16861 agtcagcttc tatgttgggg ccccgccaac ttgcattgtc tgtagaattt cctttcgaaa
    16921 ttctctttgt tggggcccca ccctaacttg cacattattg taagctgaca gaaagtcagc
    16981 ttctttgttg gggccccact agaattgaaa aaagcttatt acaagcgtat tttctttcag
    17041 tcaactactg ccaatataac attgtagtgc ctaggacatt gaatttatgg cccaggatca
    17101 gtcttatctc atcattctta atatcgttaa agaccaactt gtatcttaaa caaatactat
    17161 ctcaatatgt acaaaggttg ttatttattc agcatttttt gccgttcttc gttatataga
    17221 cttcgtcagt tatgctattt tacctttaaa atgatgttgt aaatataatg ttgtctttat
    17281 acaaaaatgt tttatacctt tttattatac agtttgcctt aatataatta aagctaacgc
    17341 attaataaaa ttaatatttt taccattaac atgtacaatg aataaagtta aaagtaatat
    17401 gacttctata gatataaata aaccctcgat tgcatctaag tcagcaacca agggttcatt
    17461 gttatatcat catagtctga tgaatctaat tatcttttat ctaattcttg ttttaatagt
    17521 tgatttacta attgtggatt agcttgacct ttagacgctt tcataatttg accaactaag
    17581 aagcccatag ctttgccttt accatttttg taatcttcaa ctgattgttc gttattgtct
    17641 aatgcttcat ttacaaattt tagaagtgtt gcttcatcag aaatttgaac taagccatta
    17701 tcttccataa tttgtttagc attaccacct ttagctgcta actctgggaa gactttcttc
    17761 gcaattttac tgctcattgt tccgtcttcg ataagtttaa tcatacctgc taaattttct
    17821 ggtgttaatt ttgtatctaa taattctact tgatttttat ttaaatattc gtttacgcca
    17881 cccattaacc agttagatgt taatttaaca tctgcgccgt gttcaattgt tgattcaaag
    17941 aaatctgaca tttctttagt caatgttaat acgtgtgcat cgtatgcagg taagcctaat
    18001 tcatttacat acttagcttt acgctcatct ggtaattcag gaattgtctg acgaacacgc
    18061 tctttccaag catcatcaat atataaaggt acaatgtcag gctctgggaa gtaacggtaa
    18121 tcatcagaac cttctttaac acgcattaaa attgttttac ctgtagattc atcaaatcga
    18181 cgtgtttctt gtccgatttc tccaccactt aacaattctt cttcttggcg tttttcttca
    18241 tattctaaac ctttacgtac atagttaaat gagtttaagt ttttcaattc agctttagta
    18301 ccaaattttt cttgaccata tggacgcaaa gagatgttag catcacagcg taaagatccc
    18361 tcttccattt taacgtctga tacaccagtg tattgaataa ttgaacgtaa tttttctaaa
    18421 tatgcatatg cttctttagg tgaacgaata tctggttcag atacgatttc aattagcggt
    18481 gtaccttgac ggttcaagtc aactaatgaa tactcacctt tatgcgtcga cttaccagca
    18541 tcttcttcca tgtgaagacg agtaataccg attcgttttg tttcaccgtc gacttcgata
    18601 tcgatatacc cattttcacc aattggttga tcaaattgag aaatttgata tgcctttgga
    18661 ttatctggat agaaatagtt cttacggtca aacttagatt ctgttgcgat ttccatattt
    18721 agtgccattg cagcacgcat tgcccagtct actgcacgct tattaacaac tggtaagaca
    18781 cctggatatg ctaagtcgat aacatttgta tttgagttag gttctgctcc aaaatgcgct
    18841 ggtgatggag aaaacatttt tgagtccgtt tttaactcta cgtgaacttc aagtcctata
    18901 actgtttcaa aatgcatgat ttccactcct tataattttt cataaacgtc atgtaaattg
    18961 tattgtgttt catattgata agcgacacga tataacgttt tttcatcgaa tggtttacca
    19021 atgaactgta aaccgattgg tcggccattt gattgtccac aaggaacaga aataccaggt
    19081 aatccagcta agtttactgg tgttgttaat aaatcattgg catacattgt taatggatca
    19141 tcaatttctt cacctaaatt aaacgcagtt gtaggcgctg ttggaccaac tactacatca
    19201 taattttcga atactttatc aaagtcattt ttaatcaatg ttctaacttt ttgagatttt
    19261 ttatagtaag catcgtagta acctgaactt aatgcaaatg tacctaagaa aatacgacgt
    19321 tttacttctt taccgaaacc ttcagatctt gacattttat ataattcttc taatgaatga
    19381 gcttctttag aatgataacc ataacgaatt ccgtcaaaac gagaaaggtt tgacgaagct
    19441 tctgatgatg caatcacgta atatgatgga ataccaaatt tagtatttgg caatgatact
    19501 tcctcaacga cagcacctaa agattttaaa gtttctacag cgttttgaac tgcttctttt
    19561 acgtcatcag ctacaccttc acctaagtat tctttaggta atgcaacttt taatccttta
    19621 atatctttac caatttcaga tgtaaagtct acatcatcaa caggtgcact tgtagagtca
    19681 ttagcatctg caccagaaat agcttctaat acgattgcat tatcttttac atttcgagtc
    19741 aatggaccaa tttggtctaa tgaagatgca aatgcaacta atccaaatcg agatacacga
    19801 ccgtatgttg gtttcatacc gacaacacca caatatgcag ccggttgtct aattgaacca
    19861 ccagtgtctg aacctaagct aaatggtact aagccagctg caactgctgc tgcagatcca
    19921 cctgatgaac cacctggtac tgctttatgg tcaaatgggt taactgtttt tttgaaataa
    19981 gatgtttctg ttgaaccacc cattgcaaac tcatccatat ttaatttacc gattaaaacg
    20041 gcattctcat tatgtagttt ttccattaca gtagattcgt aaattggcac aaaaccttct
    20101 aacattttac ttgcacatgt tgtttctaat ccgtttgtaa taatgttatc ttttataccc
    20161 attggaatac caaataattt gccatccatt tggtcttttg cttgtaattc atccaattct
    20221 tgcgcttttt taattgcatt ttctttatcc agcgctagaa aagacttaat tgttggatca
    20281 gtctcttcaa ttgcatcata tatatcttta acaacatcag atggtttgat ttttttgtct
    20341 tttattaaag ttaataaatt ctcaaccgat tcgtagcgaa tgctcatctt acgcgtcctc
    20401 ctcattcatg attgtaggca ctttaaattg tccatcttct gtttctttgg catttttcaa
    20461 agctaattct tgcggaatac ctttaattgc tttatcttca cgtaaaacgt tttgtaaatc
    20521 taaaacgtga tatgtaggtt caacgccttc tgtatcagcg ctatcatttt gttttgcaaa
    20581 atctaaaatg ctttctaatg tgttggccat ttcttccgtt tcttcaggag aaatttgaag
    20641 tcttgcaaga ttcgcgatat gctcaacttc ttcacgtgtt acttttgtca ttaataaaag
    20701 cctcctttaa gtcattcatc actaaattgt atcaaatttc caattaaaaa tctaagtatt
    20761 tatgaggtac tactttaatt tcatataaac tgtataaaca ttatcattcg tttaccaaat
    20821 cattttttat gaaaacaaca cccttttatt attagacaac ccaattcaat attatgatta
    20881 tgcaatttta attataaaaa tttgctcata tctaaaaaat acgaaagcgt tttctatatt
    20941 ggtatacaag tatttcaaaa agaataaatt taattttcct acttttctaa acatttatct
    21001 ttatgtataa tgttttcaag taactaaatt ataaattaaa taaagggagt gtttatcatg
    21061 cttacaatgg ggacagcatt aagtcaacaa gtagatgcca attggcaaac ttatattatg
    21121 attgccgtct acttcttgat actaatcgtt attggctttt acggttacaa gcaagcaact
    21181 ggtaacctaa gcgagtacat gttaggcgga cgtagtattg gaccgtatat tactgcatta
    21241 tcagctggag cttcagatat gagtggatgg atgattatgg gtctacctgg ttctgtctat
    21301 agtactggtc tttcggcaat gtggattaca atcggcttaa cattaggtgc ttatataaat
    21361 tactttgttg ttgctcctag acttcgcgtt tataccgaat tagctggaga tgcaattaca
    21421 ttaccagatt tctttaaaaa tcgtttaaac gataaaaata atgtgttaaa gattatttct
    21481 ggattgatta tcgtagtatt ctttacatta tatacacatt ctggtttcgt atctggtggt
    21541 aaattatttg aaagtgcttt tggattagat tatcatttcg gtttaatatt agttgctttc
    21601 attgtcattt tctatacttt ctttggtgga tatttagctg tatcaattac agatttcttc
    21661 caaggtgtca ttatgttaat tgcgatggtt atggtcccta ttgttgctat gatgaattta
    21721 aacggctggg gaacgtttca tgatgtagca gctatgaaac ctacaaattt aaatttattt
    21781 aaagggttat catttatagg aattatctct ctattttcat ggggattagg ttatttcggt
    21841 caacctcata tcattgtaag gtttatgtct attaaatcac acaagatgct acctaaagct
    21901 agacgtttag gtattagctg gatggctgtt ggtttattag gcgctgtggc tgttggttta
    21961 acaggtattg cattcgtacc tgcttatcat attaaactag aagatcctga gacattattc
    22021 atcgtgatga gtcaagtact cttccatcct cttgtaggtg gtttcttact tgctgcgatt
    22081 ctagcagcaa ttatgagcac catttcttca caattacttg taacgtctag ttctttaact
    22141 gaagactttt ataaattagt tcgtggtgaa gaaaaagcta aaacacatca aaaagaattc
    22201 gttatggttg gacgtttatc tgtattagca gtagcaattg ttgccatcgc gattgcatgg
    22261 aatccaaacg acacaattct aaacttagta ggtaatgctt gggccggatt tggtgcatcg
    22321 ttcagtccac ttgtgctatt tgcactttac tggaaaggtt tgacacgtgc cggtgctgta
    22381 agtggaatgg tttcaggtgc cttagtcgtt atcgtttgga ttgcgtggat taaaccattg
    22441 gcacatatca acgaaatatt cggcttatat gaaattattc ctggatttat tgtaagtgta
    22501 atcgttacat atgttgtaag taaacttact aaaaaacctg gtgcatttgt tgaaactgac
    22561 ttaaacaaag ttcgtgacat cgttagagaa aaataattca taagtcttaa caaattaaaa
    22621 aggtactaat gttaatcaaa attatgacta acattggtac ctttttatta tctttaattt
    22681 acaattaatt actgtaaata tgaacttgcg gttctttgtc atcttttgtc ttactaatta
    22741 aagcacgtgg ttggttacca tctttaatcc gaatttcata gttatcgatt ttatcgaaat
    22801 atttattcgc ttgttcagta acgtactgtg taataccaat tgtttcagct tgtccatagt
    22861 aatcgattgg taaatctact actaatcgtt gtggcttttt atcaacaaat ttaacttttc
    22921 ctactgcttg tgtgaaatta gaaaaatatg attgcaaatt atcattaaat tgcttgaaat
    22981 tattatttaa attttcatca taatctgctg ctgttgaaga aggtaataaa gctgattttt
    23041 cattgatatt atgccattca ttaagctttg tttgactctt ttctgcagtc gcttgagtga
    23101 taaattcacc tggtgtgatt gaatcttcac ttgattgctt ataaattgca aaatgaattg
    23161 gtatatcttt taaatcatca ttttcacgta accttgataa tatctcacta gccatttgtt
    23221 taccttgctt tttaacttcg ctatcatcta gttttttact aaaagtcggt ccatcttttt
    23281 cttttttata gtaataaaca ctattcatag ctaaaccaat cgtcatacct ttaatattct
    23341 taccttttgt atctccacca ccataaaaat cttgctctaa aatgttagat aaataggctg
    23401 gtgatttttc tgcaatcttt tcaggatctg tttcaccttc gtgtgatgga ttaagtccta
    23461 aattttcatt cgctttcttg tcttttttat ctttttcaga cattttatcg atttcacgtt
    23521 ttgtatactt aggatttaaa taggcattaa ttgttttctt gtccaaaaat tgaccatctt
    23581 gatacaaata tttgtctgtt ggaaacactt ctttacttaa gttcaataaa ccatcttcaa
    23641 agtcgccgcc attataacta tttgccatgt tatcttgtaa aagtcctctt gcctggcttt
    23701 ctttaaatgg taacaatgta cgatagttat caccttgtac atttttatcc gtagcaattt
    23761 cttttacttg atttgaacta ttgttatgtt tttgattatc ttttccagcc tggtcatcct
    23821 tatggttacc acaagcagcg agtataaaga tagctgtaat caataatact aatgtacgct
    23881 tcatcgacat acccctctaa ctatttaatt cattttgctt atctacaaat tgttgctctg
    23941 tccaaatttc aatacctaaa ctttgtgctt ttgttaattt tgaacctgca tcttcaccag
    24001 caataacgac atctgtattt ttagtaacgc tacttgtaac tttagcacct tgtgatgcaa
    24061 gccatttaga tgcttcattg cgtgtcattt gatgtagctt accagtcagt actatcgttt
    24121 taccactaaa ttcaggatgt ccttcaatat ctgatgtttt gataccttta taaatcatat
    24181 taacatgttt atcttttaat ttttgaatta aagcacgaat atcttcattt tctaaataag
    24241 taactacaga ttgtgctact ttatcaccta tatcatgaat ttctactaat tccgcttcag
    24301 ttactgttag taatcgatct atcgtttcat atttttctgc taacacttgg ctcgctttaa
    24361 cacctaaatg cctaatacct agaccgaata ataaattttc taaagagttg tcctttgctt
    24421 gttgaatggc agctaataaa ttatcaactt ttttctgccc cattctgtct aaaggtaata
    24481 aatcttcttc tgttaaatag aaaatatcag caacatcttt aattaattcg ctttgataaa
    24541 gctgttgaat aattttagtg cctaaaccat caatattcat ggcttgtctt gatacaaagt
    24601 gaatcaatcc ttcaacaagt tgtgcttggc attttggatt aatgcaacga agtgctactt
    24661 cgccttcaat acgtactaat tcatgtccac aacttggaca atgggttggc atatgatatg
    24721 tgacagcatc ctcaggtcta cgttctggaa tactacgtac aacttcaggt atgatgtcac
    24781 ctgctttttt cactacaaca ctatcaccaa ttcgaatatc tctgtcatga attaaatctt
    24841 cattgtgcaa ggatgctctt gatacagttg taccagctac ttttactggt tctaaaatag
    24901 cagtaggtgt gactacacct gtacgtccaa tacttaattc aatatctaat aatttagtta
    24961 ccacttcctc agctgggaat ttataagcaa tggcccatct tggtgatttt tgtgtgaatc
    25021 ccatttcgtc ctgctgatct aaatcattaa cctttatcac aataccatcg atatcgtatg
    25081 gcaatgattc cctttggctt gtccattttt caatatattc taaaacgcca tcgatatcgc
    25141 tgacgcgcgc tctattttta ttcgttgtaa aacctaattt atctaactca tctaatgctt
    25201 cactttgcga acgcgcattg aaatcggtga aatcattgac actatatata aatacgctta
    25261 gctttcgttt tgccgttaat ttagaatcta actgtcttaa tgaccccgca gctgcgtttc
    25321 ttggatttgc aaataactgc tcatcatttt tttctttttc ttcatttaat cgtaaaaatg
    25381 aacgtctcgg catatatgct tcaccacgaa cttctacatt taatggttct ttcattttca
    25441 aaggtatcgc atgaattgtt tttaaatttt cggtaatatc ttcacctgtt gttccatcac
    25501 cacgtgttaa accttgaacg aagtatccat caacatattt caatgatact gctaagccat
    25561 caatttttaa ttcgcacata tattcaacgt tgccaatttg ttcacgtatg cgttggtcga
    25621 attttctcaa atcatcctca ttaaatgcat tccctaaact taacattggc gtgtcatggt
    25681 tgactttatt gaaagaggct tgggcttcac caccaactct aaccgttgga gaatctacag
    25741 tcttaaactc aggatgctcc tcttctattt taatcagttc atgaagtaat ttgtcatatt
    25801 cactatctgg tacagatgga ttatcctcta catagtattc ataactgtat tgatttaata
    25861 aatcatgtaa ctcgttcaca cgagacgata aatcagccat cccttaatcc tccttttttt
    25921 caattggtgc aaattgcgct aacaaacgtt ttggcccttg tgatttaaag ataatatcta
    25981 gttcgattga gccatttttc tcgtttacat tactcaccat gccttctccc caggctttat
    26041 gcatcacttt gtcacctaca ttccaatcag atgacgatac ttgttttttc gttgacgttg
    26101 ttcgttgact aaatccacgt ttagcaaaag gttttgcctt aggttgtatc gtttgtcgtt
    26161 tgccacttga atgattttct aatagtgatt ctggaatttc ctttaaaaat ctagatggca
    26221 tatttgactg agggcgacca aataacattc ttgatgtcgc atgagtgatg tataacacct
    26281 cttcagccct tgtgattgct acataacaaa tacgacgttc ttcttgcatt tcatgatcat
    26341 cttcactctt aatcgctcta atatgtggga ataaagattc ttccattccc attataaaga
    26401 cgattggaaa ttcaaggccc ttagccgagt gcatcgtcat tagtgttacg ccattttcag
    26461 tatctgcctc atcaatatca gctactaacg ataaatccgt taaaaagtta attaatgact
    26521 gttcttctaa tggggtattt tcctcatagt cttttggtac tgacataaat tcatcgatgt
    26581 tttctaatct acttcgagat tctagtgtat tttcacgttc aagcatttct cggtagccag
    26641 acttttgtaa tacttcatca actatttcat gaatttctaa aaattcttgt tcttttatca
    26701 agctttggat taactcgtaa aaattaagac actcttgtgt cacctttttt gacaatccga
    26761 taaaatcagc ttctccaagt gcatcaaaca tactgatatt gttttgaagt gcataatttt
    26821 gaactttttc aacagatgaa ggacctacac ctctttttgg aacattaata atacgttgca
    26881 aactaatgtc atcattacta ttggcaatta tacgcaaata acttaataaa tctttaattt
    26941 ctttacggtc atagaacttt tggccaccaa ccattgtgta tggcatatta gatttcatga
    27001 atgtttcctc aagtacacgt gattgtgcat ttgttctata taaaattgcc atatcttgat
    27061 atttcttacc attacgttga tgcttcataa tttctcgtat tacaaattcc gcttcatcac
    27121 gttctgtcat tgcttcatag taatgaattt tctcaccatt cgtatttgca gtccacagtc
    27181 cttttggctt acgttcagaa ttatttttaa tcacttcgtt agccgcattt aaaatcgttt
    27241 tcgtcgaacg ataattttgc tctaaaaaga ttgtattcgc ttctgggtag tctttttcaa
    27301 atgataagat attttgaata tcagcaccac gccaaccata aattgactga tctgagtcac
    27361 ctacaacaca taagttttta aacttacttg ctaataattt aactaatgtg tattgtgctt
    27421 tattggtatc ttgatactca tctacatgaa tatattggaa tttgttctga taatattcta
    27481 gaacttctgg tacacgctca aataagttaa tcgttgtcat aataaggtca tcaaaatcta
    27541 acgcttcatt acgtgacaat tggcgttgat atccactata aaccgttgct accatttgcg
    27601 agtgataatc tgtggcttct ttttgagcat ctgcaggtgt tttaagttca tttttcaaat
    27661 tactgatcgc accgataaac atacgtggtt caaacttttt actatcaata ttttcatttt
    27721 ttaagacgtc tttaataaca gatttttggt ctgtaggatc aattatcgta aaattgcgtt
    27781 ctataccaat tcgatctgca tcacgacgta aaatacgcac acacattgag tggaatgttg
    27841 acatccaaat cacttctgct tgatcaccta ctaatttttg aacacgttct ttcatttctc
    27901 ttgctgcttt atttgtaaaa gtaatagcca aaacattgta tggtgagaca tctttttcgt
    27961 ctaataaata agcaattcta tgtgttaaaa cacgtgtctt ccctgaacca gcacctgcca
    28021 taattaacaa tggtccttct gttgtcttta cagcttcact ttgctctgta ttcatatgat
    28081 ttaataacgc attcatttac tagactcctt tatttttact gtttttaaag cttttttaat
    28141 atctttataa ataatatcac cgacgataat tgtatctgca atagctgcca tctctgtcgc
    28201 ttgttgttct gaggaaatac cgccaccata aaaaagttgc gtttctgtta gatgttctga
    28261 gacagcttga acctttgata cgtcgccata aatgccacta tactctatat acataaccgg
    28321 taatcgatac agatgattga ccatttgggc atatgcttct aaatcttctg ttgttaaatc
    28381 tgtatttgct ttggtatgtt ttgccacttt gctatcagca ttgcacacga catacccttc
    28441 aaatattact tcttcaaaat ctatactatg tccatatgtt ttaagcgctt ctaataatgt
    28501 accattgtga aatgcaacat ctgtactgtt cagtactgta ggtacaaaat aaaaatcaaa
    28561 accaggcatt acactttcga tgtttgatat ttcaagtact aaaggcaatg ggtatcttct
    28621 taccctgctc attaaatgaa tgacattatc ttcagtaacg tcatcagttc caccaatcat
    28681 aattgcatct gtttgagaca tacaaatcgc atctaaatca tcatctgaaa tatgcttagc
    28741 tgggtctaat ttaaaaatat ggcgccattt tttaatgtca tacattttcc gaaactcctt
    28801 ttttaacata catatgatta tagcattttt aagtgtcgag ttctaaagac ttaatatgaa
    28861 atttaagttc ttttactctg taaatttttt cgtgaaaaaa caatgattat ctaccttatt
    28921 agtacagata gataaccatt gtttatcgtt cgtttatcgt tcatttatta ttcatttgtt
    28981 tcaatattca tacgatctaa aatcattgta taagcatcat taccccattg taatgaacgc
    29041 ttcactctag aaatcgttgc agtcgatgct ccagattctt gttcaatcgt tgcataggta
    29101 taaccttgct taatcatttt agcaacttgt aatcgttgag acagtgattg aatttcattt
    29161 acagtacaca aatcatcgaa aaattggtaa cattcttctc tattttctaa cgttagtatt
    29221 gcatcaaaca attcatctaa cgctgcacct cgtaattttt caatttgcat atcgaaacaa
    29281 cccctataat tttattcttt atgtcatttt aacgcattaa cctattaaag tgtagcgatt
    29341 tatattttat taaatctgaa tcgttacttt atttaatttt atgctaatcc agcgcgttcg
    29401 aaaatagtgt caacttgatt caaatgatgt ttaggatcga aacattcatc caattcttct
    29461 tttgttaaaa cacttgtaat agactcatct tgttcgatta attcacggaa aggtgttttc
    29521 gtttcccaag atatcatcgc ttttggttgt actttgtcgt atgcttcttc acggaccata
    29581 cctttattaa ttaatgctaa taagacacgt tgtgagaaaa tcagaccaaa tgttttatct
    29641 atgttattac gcatattatc ttcaaataca gttaaacggt ccacaatatt tgtgaaacga
    29701 ttcaatgcat aatctagtgc tattgtaaca tctggtaaca taatacgctc agctgaagaa
    29761 tgagaaatat ctctttcatg ccataatggc acattctcat aagctgtagt aatataacca
    29821 cgaattactc ttgaaatacc tgtgatattt tcagaaccaa ttggatttcg tttatgaggc
    29881 attgcagatg aacctttttg accttttgca aatgcttctt caacttctct tgtttcggtt
    29941 ttttgaaggt tacgaatttc aacggcaaat ttttctagtg aagttgcgat taatgctaat
    30001 gtcgcaatat agtatgcatg tcgatcgcgt tgcaatgttt gcgttgatac aggcgctgtg
    30061 ccaataccta aatgtttaca cacataactt tctatttcag gaggaatgtt agcaaaagta
    30121 cctactgcac cactcatttt ccctacttca atttcttctc ttacttgttt gaaacgttgt
    30181 aagttacgtt gcatttccgt gtaccacaat gccattttga caccaaatgt agttggttct
    30241 gcatgcactc catgtgtacg tcccatcatc aatgtatatt tataattttt tgctttttca
    30301 gctaaaacgt cgataaatct ttctaaatct ttttcaataa tgtcatttgc ttgtttaata
    30361 acgaaactta aagctgtatc tacaacatca gtagaagtta aaccataatg tacccactta
    30421 cgttcttcac ctagcgtttc agaaacttgt ctagtaaagg ctacaacatc atggcgcgtt
    30481 tcttgttcaa tttcttgtgc acgttcgaca tttacctttg cgttttgacg aattttttgt
    30541 acgtcagctt tcggtatatg tcctaattca ctccatgcct cacatgctaa aatttccact
    30601 tctaaccatg cttcatagcg attttgatca gtccaaatat tagacatttc ttctctagaa
    30661 taacgttcaa tcattttttt aactcctatc gtatgtatac tcgaattttc aaaaacagaa
    30721 catttttaat aaaaataaat aaaaagttcg tttttaactc tactcttcac agtttaacag
    30781 aattggttat aaatgtataa aaaatttccg tatattctca ttctatttta ttttttacat
    30841 taatacaatt atctatggca gattaatact gatttctaat tgtaaatgtt cgcaacactt
    30901 taatctaatt ggtatttata atgatccatt aagaatatat tattgcattt tagtttttct
    30961 cccctcacct agactatcca cctttttatt acaattagga caatcaatag ctttagtaat
    31021 accagatatt attcacttta cttctcatat tgtatagaat tttcaacaat ctacttttcc
    31081 tagtttcata atattccttc ttacattcca taatgtgttt taaaattatt cgcttctaat
    31141 ataacaaatt ttctactcac aaaaagaatt gcataggtat ggcattttaa atataagcaa
    31201 ttaatattga actgctcgag tcgtataaat acgaaagtga ttatagaaaa gtactcagag
    31261 attttcttaa tagtaaattt ttatagacag acatcaatta actacgtttc tttcaaaatc
    31321 gactaacata ttttcatgaa acgttcgatc atattaatta ttgaataaga tgtttgcaat
    31381 ttagtaaata agctactaaa ttattgcata aactgataat gtctaataat taaatatttt
    31441 ttaaacatag attaaaaatt tatattgaaa ttcttaacta aatttaatat aattatttta
    31501 taataaaaga aaggagtttt tatatgaaaa gaaactttcc aaaattaata gctttaagtt
    31561 taatattttc tttaagtatt acccctattg caaacgctga gagcaattca aatatcaaag
    31621 caaaagataa gaggcatgtg caagttaatg tcgaggacaa gagtgttcca actgacgtac
    31681 gtaatttagc gcaaaaagac tatctttctt atgttacttc acttgataaa atctataata
    31741 aagagaaagc ctcgtataca cttggtgaac cttttaaaat ttataaattc aacaaaaaga
    31801 gtgatggtaa ttattatttt cctgttttga acactgaagg taatatcgat tatatagtta
    31861 caatttcacc aaaagtcact aaggattcta gttcatcttc taaatacaca atcaatgttt
    31921 cttcattttt atctaaagcc ttaaatgaat ataaagatca acaaattact attttgacaa
    31981 actcaaaagg ttactatgtg gttactcaaa atcataaagc taaactagta ctaaaaacac
    32041 cacgtctaga agataaaaag gcaaaaaaga ccgagagtat acctactggc aataatgtaa
    32101 cgcaactaaa gcaaaaagct tctgtcacta tgccaactag tcaatttaaa agtaataatt
    32161 atacttacaa cgagcaatat gtaaataaac ttgagaattt caagataaga gaaactcaag
    32221 gtaacaatgg ttggtgtgca ggctatacga tgtctgcatt actaaatgcg acttataata
    32281 ctaacaaata tcatgcagaa gctgtgatga gatttttaca cccaaactta caaggacaac
    32341 aattccaatt tactggttta acgccaagag agatgattta ttttggacaa actcagggaa
    32401 gaagtccaca acttcttaat agaatgacta catataatga agttgacaat cttactaaaa
    32461 ataataaagg aattgctatt ttaggtagta gagtagaatc aagaaatggt atgcacgcag
    32521 ggcatgctat ggcagttgtt gggaacgcta aattaaacaa tggacaagag gttataatta
    32581 tatggaatcc atgggataat ggttttatga ctcaagatgc taaaaacaat gttattccag
    32641 tatccaatgg cgaccattat caatggtatt catctattta tggttattaa aatttgtatt
    32701 ttagaaaggt aaataatatt atggaacaat ttgaattatt tagtattgat aaattcaaat
    32761 gtaattcaga ggctaagtat tatcttaata ttattgaggg agaatggcat cctcaagatt
    32821 taaatgatag ccctttaaaa tttattctca gtacctcaga cgattctgat tacatttgca
    32881 aatatataaa tacagaacac aaacaactca cattatataa taaaaataat agctcaattg
    32941 ttattgaaat atttatacca aatgataata aaatactact aacaattatg aacacagaag
    33001 ctttaggaac ttctcctaga atgactttca ttaagcataa ataataagac tatctagata
    33061 tatttaagta aactattact cttattaaag gactctagga agaaaaaatt tcatgcaatg
    33121 agattttttt agaaaaagcg acgatatata taatttaaca atgattttaa aatattaaga
    33181 acccccagca tatactttat atagcttgct gggggtttct catacaatat ggatcgagca
    33241 tcttgcatgt ataaacacat aggctattca aagtaaaatt actcaaactt tgaatagcct
    33301 ttcctcctca aatagtcttt atttaaattg gattatgtta ttggattttc ttacctacaa
    33361 aaatgatttc ttgatgtgat acttcaaccg tgccatcttt ttgctcctga aacacgggtt
    33421 tttcaatacg ttttaccggc atataccctt gcattttcat tcgtgacaaa caatctgcaa
    33481 ttgtttcgtt ctcatttact ttgaatttca tgttctacta cactttccag ttcttctggt
    33541 tcatagtctt taagctttct acgacgaatg cctttagtcc agaatccacc atgtatgttt
    33601 cgaggttcat acgcaatgat aaatgctttc ggatctaaat tttttatcgt atccatcaat
    33661 ttacgttcat attttcttgg tgttaaaatt tgcatcacca tacgactacc atctctacca
    33721 aatgcagcat agtgcgtaac gccatatcct aaatttcgaa gttcattcgg taaatctaac
    33781 tcatattctg ctgaagttac atttacaact gtataaccta atgccagttt ttcttctatt
    33841 ttcataccaa caatgatacc tattgaaaaa ccaaatgcgt aggcaataat attttgaata
    33901 tggtctaaat tagacataac caaacctaaa ccaacgatat aaactaatac ttctaaaaaa
    33961 ctaactgatg cagcaatata acgataacct ttcaacgtta aaattgttcg catcgttaaa
    34021 aacgttacat aacaaacgtt aatgataaat atagttagta ccattaacca tggattttct
    34081 gttacgaatg acataaggcg ccccactctc tttcaattac actttaaata tcaaactgct
    34141 tcaaactaac cttaataaat gcatattaat ataatatagt acgaaaactc tcataaataa
    34201 atagcgaatt tgtcataaat aaaatttatt ctcaaatgaa catacttact ttatttcata
    34261 cattatcaat ttatatactg aagtagccta gttgaatgtg gtttaacgtt attaattcta
    34321 actcatattt aatttaaggc acacgttaga agaaaaaatt aaattaggat tttggccacg
    34381 tgtatcttgt atatgcaagt tcacgtttgt gtgcattccg tatataatga ttttcaatta
    34441 ctttttgttc ttctggcgta actggcttac cttctaaata attatcaatc gcctcataag
    34501 ttacacctaa tgcatcttca tctggaagct gtggtttgtc atcttctaaa tcagcagttg
    34561 gtgttttttc atacaattgt tttggtgcac caagataagc taataactga cgaccttggc
    34621 gcttattcaa accgaatata ggtgcaatat ctgcagcacc atcaccatat ttcgtataaa
    34681 aaccagttat attttcagct gaatgatctg ttcctactac aataccttgt cggtttgaag
    34741 caattgaaaa ttgtactttc atacgttcgc gagccttttc attacctttt tggaagtctg
    34801 ttaggacaat cccagcttct ttcaatgatt gcacactttg atcaactgca ggcttaatat
    34861 tgactgttac tatttcatct ggctcaatga attgtaaagc ttgctcaact tcatcagcat
    34921 ctttttgaac tccataaggt aatttaactg caataaacgt acaatcaatg ccttcttcac
    34981 gtaattcgtt aacagacatt tgcactaatt ttccaactaa tgtagaatct tgtcctcctg
    35041 aaatacctaa cactaaagat tttataaatg aatgtgattg gacataactt tttataaatt
    35101 gttttaactc agcaatttct tccgcactgt cgatacgctt tttcactttc atttcttgta
    35161 caataacgtc ttgtaattta ctcattatct tcttccatct ccttaacatg ttccgccact
    35221 tcgaaaatac gtttatgttt attatcccaa catgctttac ttaaatcgac tgggtactct
    35281 tgtggattta agaaacgctt gttttcatcc caaatggatt gtaatccttg tgctaaataa
    35341 tcacgtgatt catcttctgt tggaatttga tacactaatt taccattttc ataaatatta
    35401 tgatgcaaat caatggcttc gaaagatttt ataaatttca ttttataagt atacactgga
    35461 tggaataatt ttaaaggttg ttcatcgtat ggattttcat tttccaaagt aatataatcg
    35521 ccttctgcct tacctgtttt cttgtttata atgcgatata catttttctt acctggcgta
    35581 gtaacctttt cagcgttatt tgataattta atacgatcac tatatgaacc atcttcattt
    35641 tcaatagcta caagtttata tactgcacct aatgctggtt gatcgtatcc tgtaatcagc
    35701 tttgtaccaa cgccccaaga atctactttt gcaccttgtg ctttcaaact cgtaatcgtt
    35761 tcttcatcca aatcattaga cgcgataatt ttagtttcag taaatcctgc ttcatcaagc
    35821 atacgtctgg cctctttaga taaataagcg atatctccag aatccaatcg aatacctaca
    35881 aagttaattt tgtcacctaa ttcttttgca acttttattg catttggcac gccagatttt
    35941 aaagtatgga atgtatctac taggaacaca caatttttat gtctttcagc atattttttg
    36001 aaggcaacat attcatctcc ataagtttgg acaaatgcgt gtgcatgtgt accagacaca
    36061 ggtataccaa ataatttacc cgccctaaca ttacttgtag aatcaaagcc cccgatgtaa
    36121 gcagctctag cgccccacaa tgctgcatca atttcttgcg cacgacgtgt accaaactcc
    36181 attaatttat catttgatgc aatttgacga attctactag cctttgttgt aattagtgta
    36241 tggaaattta caatgtttaa taaaattgtt tctattaatt gcgcttgaat caatggtgct
    36301 tctacgcgta acaatggttc gttaccaaag cataattcgc cttcttgcat cgaacggatg
    36361 ctgccagtga attttaaatc ttttaaatat gataagaaat catccttgta gccaatagac
    36421 tttaaatatt ccaaatcaga ttctgaaaat ccaaaatgtt ctataaaatc aatgacgcgt
    36481 tttaaaccat taaaaacagc atagccacta ttaaatggca tttttctaaa atataaatca
    36541 aatacagcca ttttttcatg aatattatca ttccaataac tttcagccat atttatttga
    36601 tataagtcat tatgtaacat taaactgtcg tcttctaatt ggtacactcg tatctctcca
    36661 atcgacctaa atattttctt acattttatc ataattcatt tttttatata cataagagcc
    36721 ccttaatttc catactttta attaaaatca accaacaatt taatgacata tacataattt
    36781 ttaagagtat tttaataatg tagactataa tataaagcga ggtgttgtta atgttattta
    36841 aagaggctca agctttcata gaaaacatgt ataaagagtg tcattatgaa acgcaaatta
    36901 tcaataaacg tttacatgac attgaactag aaataaaaga aactgggaca tatacacata
    36961 cagaagaaga acttatttat ggtgctaaaa tggcttggcg taattcaaat cgttgcattg
    37021 gtcgtttatt ttgggattcg ttaaatgtca ttgatgcaag agatgttact gacgaagcat
    37081 cgttcttatc atcaattaat tatcatattg cacaggctac taatgaaggt aaattaaagc
    37141 cgtatattac tatatatgct ccaaaggatg gacctaaaat tttcaacaat caattaattc
    37201 gctatgctgg ctatgacaat tgtggtgatc ctgctgaaaa agaagttaca cgcttagcaa
    37261 atcacttagg ttggaaagga aaaggtacta attttgacgt gttaccactg atttaccaat
    37321 tacctaatga gtcagttaaa tattacgaat atcctacgtc attaattaaa gaagtaccta
    37381 ttgaacatga tcattatcca aaattaagaa aattgaactt aaaatggtat gcagtcccta
    37441 tcatttccaa tatggactta aaaatcggtg gcattgtgta tccaactgcc ccctttaacg
    37501 gttggtatat ggtaactgaa attggcgtac gtaactttat tgatgattac cgttacaatt
    37561 tactagaaaa agttgcagat gcgtttgaat ttgatacact taaaaataat tcatttaata
    37621 aagatcgagc acttgttgaa ttgaactatg ctgtgtatca ttcctttaaa aaagaaggcg
    37681 tatcaattgt cgatcatttg accgctgcaa agcaattcga actattcgaa cgtaatgaag
    37741 cacaacaagg tcgtcaagtt accggaaaat ggtcttggct agcaccgcca ttatctccaa
    37801 cattgacgtc aaattatcat cacggatatg acaatacagt aaaagatcca aactttttct
    37861 ataaaaagaa aaaatcaaat gctaaccagt gccctttcca tcattaagtt agtagaggta
    37921 tagcatatgc aattatatta tttaggccct aaaggtacat tttcatattt ggcttgtcgt
    37981 cagtattttt ctgaaaatga agcaactttt caaccaaagt caaatttatt tgaagtcatc
    38041 aagtcagttg cagatgatga cacatcgatt ggtgttgtcc ctattgaaaa ttctatagaa
    38101 ggaacaatca acattgtggc agatgcactt gcacagcaag atgtctttgc acacggtgaa
    38161 atacgtttag atattaactt tgcattatat ggtaatggtt cgaacaccat ttctgatatt
    38221 aaaaaagtgt attctattgc accagcaatc agtcaaacaa caaattacat acatcaacat
    38281 caatttgact atgattatgt cgacagtaca attcaaagtt tgactaaaat tgaaaatggc
    38341 gttgcagc
//
LOCUS       DAMZPP010000006        36802 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMZPP010000006 DAMZPP010000000
VERSION     DAMZPP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 36802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36802
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.006"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(95..203)
                     /locus_tag="POH73_000230"
                     /pseudo
     CDS             complement(95..203)
                     /locus_tag="POH73_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(278..1288)
                     /gene="adhP"
                     /locus_tag="POH73_000231"
     CDS             complement(278..1288)
                     /gene="adhP"
                     /locus_tag="POH73_000231"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDI6886218.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
     gene            complement(1787..2308)
                     /locus_tag="POH73_000232"
     CDS             complement(1787..2308)
                     /locus_tag="POH73_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HDI6886219.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(2336..3631)
                     /locus_tag="POH73_000233"
     CDS             complement(2336..3631)
                     /locus_tag="POH73_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDI6886220.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVSPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGKIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(3671..4147)
                     /locus_tag="POH73_000234"
     CDS             complement(3671..4147)
                     /locus_tag="POH73_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6886221.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(4316..4852)
                     /locus_tag="POH73_000235"
     CDS             complement(4316..4852)
                     /locus_tag="POH73_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDI6886222.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(5062..6000)
                     /locus_tag="POH73_000236"
     CDS             complement(5062..6000)
                     /locus_tag="POH73_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6886223.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDDNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVIENIKAADIVLSDDEIQYIDELFPIED"
     gene            complement(6453..7082)
                     /locus_tag="POH73_000237"
     CDS             complement(6453..7082)
                     /locus_tag="POH73_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6886224.1"
                     /translation="MLCEVIAIYKNPKYRIIKYNDEYLMVNIINNWLVLFIPLLNWLT
                     PKRYIKISQEELENLNTFKPAKNNAFWPALGSSVLFSVTFRKYMPLFNVRLEKTIVIA
                     IFFVVFLGILFFYLNLNRRLALSVFTMNKEKSQKMILLPNLKHFFFIIFAYLYFGGFS
                     FFTLYALITIDVQNIIIFICWGAVTMFFFFINIVPIGIKKAHVILLKKS"
     gene            complement(7222..7843)
                     /locus_tag="POH73_000238"
                     /pseudo
     CDS             complement(7222..7843)
                     /locus_tag="POH73_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
     gene            complement(7844..8005)
                     /locus_tag="POH73_000239"
                     /pseudo
     CDS             complement(7844..8005)
                     /locus_tag="POH73_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8079..8714)
                     /locus_tag="POH73_000240"
     CDS             complement(8079..8714)
                     /locus_tag="POH73_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDI6886225.1"
                     /translation="MLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWLI
                     PKKYVKISKKEFDDLNIVKPVKNKAFWPVAGSTILFGVTFRKYIPSLNIQLEKNMVIV
                     ICCAIFLGVLILFLFLNRKLRLEIYNNNSSKGKIILFPSLKNFCFTIFYYFLFGGLSI
                     MALSMLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVLSKTNTVEK"
     gene            complement(8718..10112)
                     /locus_tag="POH73_000241"
     CDS             complement(8718..10112)
                     /locus_tag="POH73_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6886226.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEQKAEKETLNKADFAVIKAYAKEHPEDDIPKSIMKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDMANDDKTVGEALSHNALTTGIGIGAGAGMTATIALFASNPVGWAV
                     IGGFVFGSVSTMLVDWAYQNNKAGIKDKADWVGHKLDDGIDVVKKSTEKAVDSVGEAT
                     KSISNHLNPMKWSW"
     gene            complement(10577..10678)
                     /locus_tag="POH73_000242"
     CDS             complement(10577..10678)
                     /locus_tag="POH73_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886227.1"
                     /translation="MCQYLCLKSVELGVIIMMIYYLKVVALTVRLRN"
     gene            10736..11176
                     /locus_tag="POH73_000243"
     CDS             10736..11176
                     /locus_tag="POH73_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDI6886228.1"
                     /translation="MNLEFNIAVHVLTFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMINTIRGKDGGYLANDRSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNNIINFYKEKTIKDVIEDIQKEDLCHENI"
     gene            11163..12485
                     /locus_tag="POH73_000244"
     CDS             11163..12485
                     /locus_tag="POH73_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDI6886229.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSSKMYGGTCI
                     NVGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDSNIDVLDFKAQFK
                     SNTEVNLLDQHGDIVDSITAPHIIINTGATSIIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAHAITDLENKGIAL
                     HTNVETTELSSDNYHTTVHTNVGNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSSLYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTILRDNIYTHPTMAESFNDLFNF"
     gene            complement(12537..12878)
                     /locus_tag="POH73_000245"
     CDS             complement(12537..12878)
                     /locus_tag="POH73_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDI6886230.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFAFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKEEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(13055..14131)
                     /locus_tag="POH73_000246"
     CDS             complement(13055..14131)
                     /locus_tag="POH73_000246"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDI6886231.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            complement(14144..15127)
                     /gene="mvaD"
                     /locus_tag="POH73_000247"
     CDS             complement(14144..15127)
                     /gene="mvaD"
                     /locus_tag="POH73_000247"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDI6886232.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDLQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYNDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            complement(15132..16052)
                     /gene="mvk"
                     /locus_tag="POH73_000248"
     CDS             complement(15132..16052)
                     /gene="mvk"
                     /locus_tag="POH73_000248"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDI6886233.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHKFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            complement(16639..17475)
                     /locus_tag="POH73_000249"
     CDS             complement(16639..17475)
                     /locus_tag="POH73_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDI6886234.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFEDENFDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            complement(17478..18464)
                     /gene="pta"
                     /locus_tag="POH73_000250"
     CDS             complement(17478..18464)
                     /gene="pta"
                     /locus_tag="POH73_000250"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDI6886235.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLNLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSFITAAQALQ"
     gene            18632..19384
                     /locus_tag="POH73_000251"
     CDS             18632..19384
                     /locus_tag="POH73_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HDI6886236.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRLVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFNQFFAIS"
     gene            19932..20696
                     /locus_tag="POH73_000252"
     CDS             19932..20696
                     /locus_tag="POH73_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDI6886237.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLASIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            20709..21161
                     /locus_tag="POH73_000253"
     CDS             20709..21161
                     /locus_tag="POH73_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDI6886238.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(21303..22787)
                     /locus_tag="POH73_000254"
     CDS             complement(21303..22787)
                     /locus_tag="POH73_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDI6886239.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLLTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVSVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(22908..23276)
                     /locus_tag="POH73_000255"
     CDS             complement(22908..23276)
                     /locus_tag="POH73_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDI6886240.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            complement(23409..23789)
                     /locus_tag="POH73_000256"
     CDS             complement(23409..23789)
                     /locus_tag="POH73_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HDI6886241.1"
                     /translation="MVNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAPT
                     SNTPHIGEQQMNHRIANHNQMPHSQITQSTHQVTAAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            complement(23790..24446)
                     /locus_tag="POH73_000257"
     CDS             complement(23790..24446)
                     /locus_tag="POH73_000257"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDI6886242.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANTYLESVGKSPINWCESEA
                     "
     gene            24630..25460
                     /gene="thiD"
                     /locus_tag="POH73_000258"
     CDS             24630..25460
                     /gene="thiD"
                     /locus_tag="POH73_000258"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDI6886243.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            25993..26160
                     /locus_tag="POH73_000259"
     CDS             25993..26160
                     /locus_tag="POH73_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HDI6886244.1"
                     /translation="MIIYRQYQHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(26361..26630)
                     /locus_tag="POH73_000260"
     CDS             complement(26361..26630)
                     /locus_tag="POH73_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886245.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLEAN
                     QCQINYVKPILINENIKFIEEHEQEIIAINDDGEIKIKISLSTKK"
     gene            complement(26633..26998)
                     /locus_tag="POH73_000261"
     CDS             complement(26633..26998)
                     /locus_tag="POH73_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HDI6886246.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYHCFNQPYSKEVPPLMCASL
                     WSKFDLFKKYANSELILTKSVINQTQKIKVDTIYVGHLEDIECRQIRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            complement(26973..28112)
                     /locus_tag="POH73_000262"
     CDS             complement(26973..28112)
                     /locus_tag="POH73_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDI6886247.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYGVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGELFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFDHGLLFKDGVTVGVDSN
                     LPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKESMIASMGIGGGLGNAALFTRF"
     gene            complement(28114..29490)
                     /locus_tag="POH73_000263"
     CDS             complement(28114..29490)
                     /locus_tag="POH73_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDI6886248.1"
                     /translation="MDVILEQLETHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTCLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPERLLNQCRKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICVKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQHDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLNKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            complement(30035..31435)
                     /locus_tag="POH73_000264"
     CDS             complement(30035..31435)
                     /locus_tag="POH73_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6886249.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVESADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIALLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFGLGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVAKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     VEHPKEALWWVKERKD"
     gene            complement(31938..32585)
                     /locus_tag="POH73_000265"
     CDS             complement(31938..32585)
                     /locus_tag="POH73_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6886250.1"
                     /translation="MKFENYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSAQDISH
                     YMGIPIEESFLKLADRPLDEATLAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQHAGVPSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            complement(32698..33246)
                     /gene="hxlB"
                     /locus_tag="POH73_000266"
     CDS             complement(32698..33246)
                     /gene="hxlB"
                     /locus_tag="POH73_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HDI6886251.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQVHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEHGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVSEQTMQQNHANLE"
     gene            complement(33248..33880)
                     /gene="hxlA"
                     /locus_tag="POH73_000267"
     CDS             complement(33248..33880)
                     /gene="hxlA"
                     /locus_tag="POH73_000267"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HDI6886252.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIMDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKEIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            complement(33957..34715)
                     /gene="nagB"
                     /locus_tag="POH73_000268"
     CDS             complement(33957..34715)
                     /gene="nagB"
                     /locus_tag="POH73_000268"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDI6886253.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDAYDMNAEASTYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFENEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            34992..35354
                     /locus_tag="POH73_000269"
     CDS             34992..35354
                     /locus_tag="POH73_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HDI6886254.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQVTYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEINEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            35368..36033
                     /gene="bshB2"
                     /locus_tag="POH73_000270"
     CDS             35368..36033
                     /gene="bshB2"
                     /locus_tag="POH73_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HDI6886255.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLASYIQKGTPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERIPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            36046..>36802
                     /locus_tag="POH73_000271"
     CDS             36046..>36802
                     /locus_tag="POH73_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HDI6886256.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLCTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDL"
BASE COUNT        11927 a         6779 c         5304 g        12792 t
ORIGIN      
        1 aatttactac tcaccttaat cttcgcataa tagaaatatt atagcataaa ataaggtcat
       61 cttgttatgc cttcttaaag ctaaataggt tcaattaatt aacaattcca tacttaatca
      121 acgcttgata tatgtaagcc attttataaa atacaaacaa aacctcaaaa ccaaaaaaac
      181 acgagatttt cactatcttt catgaaaacc tcgtgtttga ttataatgat agtgttcaga
      241 gcactacctt ctttatctat aaagtcattt gatattttta aaatttaata accattctac
      301 cagttatagt accattttcc atttcttcaa aaatatcatt gatttcttct aattttctta
      361 attgaacttt aggtgttact ttattttcag cagcaaattc aaacgcttca cgtaagtctt
      421 gtcttgtacc aacaagtgaa cctactactt caataccatc tagcactaat cttgggatat
      481 ctaagttcat tttatcaaca ggtaaaccaa cggcaacaac tcttgcacca gcttttacaa
      541 catcaaccgc ttggttaaat ggcgtcttag caactgaagt taccactgtt gcatctaatc
      601 ctttgttatc agttaatttc ataacttctg caactggatc aacatcttta gaattaataa
      661 tagcatctgc acctaattct tttgcgaatg ctaatttatc atcattaatg tcgaaggcaa
      721 caattttagc ccccataacg tttttagcat attgtaaagc taggttacct aaaccaccta
      781 taccaaaaac acctaaccat tgtccaggtt taacattact tacttttaca gctttataag
      841 ttgtcacacc tgcgcatgta atagaagacg ctgctgctgg atctaatttt tcaggtactt
      901 tcacagcata gtctgcagta acaataactt gttcagccat tgcaccatct actgtataac
      961 cagcattttt cacactacgg caaagtgttt cacgacctgt tgtacaatat tcacatcttc
     1021 cacagctttc gaacatccaa gcgatagaca cacggtctcc aatttttaat gattctacat
     1081 cttctgcaac ttcgatgact ttaccaatac cttcatgacc taaagtaacg cctgtaacat
     1141 caccaaaatc agcattctta acatgtaaat cggtatgaca aacgccacaa tattccgttt
     1201 gtaccaatgc ttcaccaggt tttaaagctc ttaacttttt gtcctcaata cttactttgt
     1261 gatctttcgt tacaactgct gctctcataa taatgtcctc cttatagtca ctccagtatg
     1321 tatgatatta attggcgatt actttcaaaa atgaaaacga ataattatag atgtaatttt
     1381 tatttgtaat agacgccaga ttaaaacata ctactgtgac tttgttttta gttagatatc
     1441 aaccgttaac ttttaacgtt tccctagtta atatgttaaa agaaatgaca taatcgatat
     1501 gctaacgtgt aatttttact tcacatatat ttatttaaag ataatttata atactcttat
     1561 cttcactctt attataatgt gaattaattc acaaagtata tagctatgat caaaaaaatg
     1621 tgtcagttat tcaaattttt tattaactac agattaattt aatttcaaaa aaattaaaaa
     1681 aactcccaat caatctaaga ctttattttc acctcaagat ttacgtcgtc attgagaaat
     1741 gaatcatatc tttatagatt aattgggaaa ttttagtttc taatatttaa ttaaatgctt
     1801 gatgtaatgc atcagaagaa tgcttccaca taccttcatc atggttcatt aaaaagtctt
     1861 ttaaaatagc tttattctcg tcaccaattt gatcaatgat aatatgatgt ttcattgatt
     1921 tatccatcat attaacatgt tcaggcatac ttttataacc acgacgaatt gctttattca
     1981 ctaataaata gcacgctgtt aaaccagcat aatatggtcc ttgttcatta cgttctgttg
     2041 ttacccaaca caaccaatat tctttagctt catcgccttc aacagcagct ttatcagtaa
     2101 tccatttcac gcctttttca actttggcgc gagcatgcat gcctccaata tcaataaatg
     2161 cttctttgtt agcaacatcg ataaaaactg gtgcaatatt gtctaagcta attgaaccaa
     2221 tatttgtacc tttatgacca tctagagggt cattttttat gatattaaaa ttaaagcctt
     2281 ttttctcaga caatgatata acctccatat ttaacgataa caattttagc cctgtttaat
     2341 taagctcatt aatcaaattt tcaatagctt ctctaattgt cttatcttta atctttgcaa
     2401 ccatttctct aggataatat aatttatagt cttggcgatt aatgcctgta atactatgaa
     2461 tgactaaaga ttgattgctt atttctctaa tcttaccatc ttgtcttaat aaatgaatcg
     2521 gtttgcgatt tgacccaggt cgatcatagt catatggcaa atccgaaaat gcttcactca
     2581 caaaataata atctggatta ataccacctg cttcaaacag ttcttgcagt tctgatattg
     2641 taataattga gccatcaaat ggaatatatt taaataagtc tcgattaata aatcgacttg
     2701 ctaaatcact taaaatggca tcatcttctt ttgtccattt ttccaaataa tacgtaacta
     2761 ccgcttcatc gagttcaaca tattgttcaa tcgtaactgt ctcttcaaaa aatggaataa
     2821 aatcatgtgg atgcaactta aattcatagc cttcattata aagctgtttt gcgcgtttca
     2881 aacaattatt aagcagcact tctccaccac gactaactgg gtggaaataa atctgccaat
     2941 acatttgata acgactcata ataaagtttt caactgcatg cataccactt tctttaatta
     3001 gtacttcatc tttagaaggt cgcattaatc ttaaaatacg ttccatatca aaagcaccat
     3061 atgacacacc tgtgaaatac gcatcacgtt gtaaataatc cattctatcc gcatcgattt
     3121 gtgaagaaat catcgaaatg accaatttat tatgatgcgt tttattaatt acttccgcaa
     3181 cttctcttgg aaactcaggc gatactttac gtaatacagc attcacctca gtatctccag
     3241 taataatcgc ttgagtgtat gcttcatggt ctgtattaaa tattttttca aaactatgtg
     3301 aaaatggacc atgtcctaaa tcatgtaata atgcagcaca taatgccaac ggtctatctt
     3361 tattgtccca tgcatcatga ccaataaatg actcatcaat taatcgtcta actatttcat
     3421 acacacctaa agaatgtcca aagcgactat gttctgctgt gtgaaaagat aggtacagtg
     3481 ttcctaattg tctaattcga cgtaaccttt gaaattcctt agttttaatt aaatcccata
     3541 tcaattgatc ttcaacatga atatatcgat gtatcgggtc tttaaaaact ttttcttcta
     3601 ctaattttaa atctacatat gcgttagtca ttattcccct ccttttcgtt taatataata
     3661 tttaatttac ttaaaatgct ttgtacataa gtgctaagtc taacttttcg ccatacattt
     3721 ctggctcata agagcgtaag attgtaaaac cttgctcttt atagtaagct actgcttctt
     3781 catttttatt atctacttct aagtaaacac cttcaaattt atcttcaaaa cgtgataatc
     3841 cttcatttaa caatgctgta ccataacctg tatgttgcga ttctggttta acataatgag
     3901 ctgataaata taattcttca ccgtaaataa agttagcaaa gccaacgatg tcattacctt
     3961 cttcaacgac taagaataat tgttcttgaa gtcttttctt taaatgatgt tcattatatg
     4021 aagcttctaa caagtgatta actgttgtcg cagcgtatat atttaagtat gtattaaacc
     4081 aagctttagt tgcgacatct ctaatttgaa caacatcttt ttcagttgct tgtcttacct
     4141 tgaacatgac tttctcccct tattaacaag ttttaataac ggcattatac cacaacttgc
     4201 tcaatactta ataaacaatg attgtctatt caatttatat atttatattt tccgttaaaa
     4261 ttaaaaataa aaaataacga agcaaaaaat cacttcgttt agtatgaggt atgtcttatt
     4321 gcaatatact attccactca gttgcacgtg ctaaggcata gttgtctttc atgatgtcac
     4381 caggcttttc agcagttcca ataatataac catttaaagt ggcacctaaa aagtctaaac
     4441 tatatttcat ttgcgtaatt gctggcttcg cttttatttt tggacaatct ccaccaacta
     4501 aaataactct aaaatccttt tcggccattt gtgccttaaa attaggatat cgtttatctt
     4561 gtaatgtttc tgaccaatgt tcgataaatg ctttcaatgg tgctgaaatg ctataccaat
     4621 acactggtga tgcaaaaata atcgtatcac tagccaatat tttatctaga atcggcaaat
     4681 agtcatcgtc atatgaagta atagtctctg ctgtatgtct cacgtcacgt atcggtttaa
     4741 actgatgttg tgtcacgtca atccattgat actctaaatc ttgcaaagcg aattttgtta
     4801 attgtgcagt attaccgttt ggtctactcc caccaaacaa aacagtaatc attttagcct
     4861 aacctcactt ttgattaata aatatctgtg tttttcgtta cctaattata ctatcataag
     4921 ctttacctac cgaatagtaa aacgcttaca acttttatat aaatttgacg aaatttcgtc
     4981 atgccttata taaagtcgtt tgtgatacgg ggctaattca tgatgaatta gatacatatg
     5041 tcaccattaa atacaattca tttagtcttc aattggaaac agttcatcga tatattgaat
     5101 ctcatcatct gataaaacga tatctgcagc tttaatattt tcaatgactt gttctgcacg
     5161 ttttgcacca ggaataatca catcgatagc tggtctcgtt aaataaaatg ctaatacaat
     5221 gttcgcaatt gaagtttgat gtgctgcagc tatgctttcc aaagctttta cgcgacgcac
     5281 attttcttca aatacacctg gtttaaaatc acgacgtgta gtacgatggt cactaaattt
     5341 agtgttctca tcatattttc cagctaaaat accggatgct aaagggaaat atggaataaa
     5401 tgtgatttgg tgatcaacac aatattgtaa tactgcctca ttttcacgat gcaataaatt
     5461 atattctaac tgtacaacat caacgtaacc atctttattt gcttctttaa gttgatctaa
     5521 tgtgaaattt gatacaccaa tggctttaat cttcccttgt tccttaagct cttgtaatgc
     5581 tgcaactgct tgatctttcg gagtgttgtc atctggaaaa tgaatataat ataaatcgat
     5641 ataatcagtt tgtagacgtt tcaaactatt ctcaacttgt tgttttaaat attccggttg
     5701 attgttctga tgtacttctt gattttcatc aaattcatga gaccctttcg tagcaatttt
     5761 aatttgctct cgcggatatt ccttaacaac ttctccaacc aattcttctg atcgttctgg
     5821 cccataaata tatgccgtat ctaataaatt aataccatga ttaatggctt gacgaacaac
     5881 atcttttcct tgttcttcat ctaagttcgg atataaatta tgcccaccta ctgcgttcgt
     5941 cccaagtgcg attggaaaca cttcaacatc agatttacct aagtttacaa attgcttcat
     6001 tagacccagc tccttatctt tataactttt ccactatcac actatcatga tttaatacaa
     6061 cttccaaatg ttagaattag ggtttgaatt taggcggtgt attagaaaaa tgttatgtag
     6121 aactcagtta aaataagtat tgcttaagtt gatattcaca tagtgcgatt gcttgttagc
     6181 ctttaaatta catgtacttt tgaatcagaa attgaaaaat catttaagaa cgacaacatt
     6241 aaagccattg caaattcaat tagaaatact ataatgtttt gatttgcata acaaggaaat
     6301 taaaactgtt aatattaaca tgggatatcc attataaaac attcatcttt gtagttgata
     6361 attctatatt tcggattttt attcattcta tctatgagtc gaatttttat attaaattta
     6421 ataattttag atttaaaaaa ctcctttatt attcagcttt ttttaagaag tattacatga
     6481 gcttttttaa tgccaatggg aacaatgttt ataaaaaaga aaaacatagt tactgcaccc
     6541 caacagataa atataattat attttgaaca tctatagtta tcagggcata taaagtgaaa
     6601 aatgaaaaac caccgaaata taaatatgca aatattataa agaaaaaatg ttttaaatta
     6661 ggtagcaata tcattttctg acttttttct ttattcatag taaaaacact taatgctaac
     6721 ctacgattta aatttaggta gaaaaataaa ataccaagaa atacaacaaa aaatatggct
     6781 attactattg ttttttccaa tctaacatta aataaaggca tatactttct aaaagtcaca
     6841 ctaaataaaa ctgaactacc taatgctggc caaaatgcat tgtttttagc aggtttgaaa
     6901 gtatttaaat tttctaattc ttcttgactg atcttgatgt atctttttgg tgtcaaccaa
     6961 ttaagcaatg gaataaataa aactaaccaa ttatttatta tattcaccat caaatattca
     7021 tcattatatt taataattct atatttggga tttttgtata tagctattac ctcgcaaagc
     7081 aatgtctcca catcccttta attttatgtg taatacattt ttcgatactt caaaagacat
     7141 taaaatatta tcaacttact atcattaaaa attttattca ttgttatttg catatcttta
     7201 atctaaattt attactacca tttaattttt aagtatgaca tgaatgtttt tatctataat
     7261 taaagctata ttcactagaa aaaaacccat cataactgca atccaagcaa aatatactat
     7321 tatgttttgg taactcattg ataatagtgc atctaggaat agcatagtca atccgccaaa
     7381 taaaatatat ccaaataatg tgaaacaaac attttttagt gtaggtatta aaaaaatttt
     7441 tgacctttta tttttagtat tataaatatt taaagatgat tttttaatta ggcgtacata
     7501 aaatattaaa atccctataa atgtgatgca acatatggca ataactaatt ttttatcaag
     7561 ttgaacgtca agtaaatgtg tatattttct taatgcaact cccagcaaag cagagctacc
     7621 tgcaactggc caaaaaacat tttttagcag gtttaacaat atttaaatcg tcaaattctt
     7681 ttttactgat tttgacatat ctttttggga tgaaccaatt gataaatgaa aaaaataaac
     7741 taatccaagt gcttattata tcgaccatta agtattcatc attatattta ataattctat
     7801 atttggggtt tttattgatg attttagatt cgcatagcaa catttaccag ctccatttct
     7861 tagggcttat atgattagaa atactttttg aaccttcacc aacaacattc ccaacactat
     7921 atacggcttt ttctgtagat tttttaacac tatcgatacc atcgtctagt ttatgtccta
     7981 tccaatcgtt tttacctttt attccaaatt catttctttg attggtccaa tatacaagta
     8041 ttgacgaaac agaactcgca gtaaatccag ctaaaccatc atttttccac cgtgttagtt
     8101 ttagataata cataaatttt tttgtcaata atcgatgaca tgtttaacag aaagaaacct
     8161 gcagtcatta ccaaccaacc aataaagcct attatatttt gaggatttaa agttaataac
     8221 atacttagag ccattattga aagaccgcca aataaaaaat aataaaatat tgtaaagcaa
     8281 aagtttttta atgaaggaaa taaaattatt ttccctttac tagagttatt attataaatt
     8341 tccaatctta gcttacgatt cagaaataaa aaaagtatta aaacacccag aaatattgca
     8401 caacatatta caatcaccat gtttttctct aattgaatat taagtgaagg aatatatttt
     8461 ctaaacgtaa ctccgaacaa aatagtacta cctgcaactg gccaaaaagc tttattttta
     8521 acaggtttga caatgtttaa atcgtcaaat tcttttttac tgattttgac atattttttt
     8581 ggaattaacc aattaatcat aggaagaaat aaagttaacc aggtacttac taaatcaatc
     8641 attaaatatt catcaccata tttaataact ctatacttag gatttttgtt gataacttta
     8701 gattcacata gcaacattta ccagctccat ttcatagggt ttaaatgatt agaaatactt
     8761 tttgtagctt caccaacact atctacggct ttttctgtag attttttaac aacatcgata
     8821 ccatcgtcta gtttatgtcc tacccagtct gctttatctt ttataccagc cttattattt
     8881 tgataagccc aatctacaag cattgtcgaa accgaaccaa acacaaagcc acctataaca
     8941 gcccatccta ctggattact tgcaaataac gcgatagtag cagtcatacc cgcacctgcg
     9001 ccaataccta ttcctgtagt taaagcatta tgcgacaatg cctctccaac tgttttatca
     9061 tcgtttgcca tatcatcata catacctata ccaaatccta cacctgcaac acctttgcct
     9121 aggtattttc cgccttttgc aaatttacta ccattttcta taaacacatt acctgatgta
     9181 cgtttgactt ccacaaatga atttggaccg gctgggcctt tcactccacc tgctgtatta
     9241 ataaatacac cgaatttact tgcatttata ccgccttgct ctaaaagtgt tgacgtaata
     9301 tctaatccta tatctcgttt aatactatct ttattgtcat ttatatattt cattatactt
     9361 ttcgggatat cgtcttctgg atgttcttta gcatatgcct ttataacagc aaagtctgct
     9421 ttatttaaag tttctttctc tgctttttgt tcaattttcc ccatagcaac tttcaaatat
     9481 ttttcatgac ttgctttagc ccaatcaagt tctttacctg aaggtatatt aaattgattt
     9541 gttgaaaagt tccaaaaatt ttgcgcttgg gtaagtcctt gttgtacaat tttttgaaat
     9601 tcttcaactt ctttaaatat ttctggtgat ttttgattaa actcacgcaa tttgcgtagc
     9661 ttctcttcta attcatgttt ttgttgacct aatgttcgta ttatttgttg gttcgatgaa
     9721 atggcttgct gattatcgga agcatgcttt ttcaaattgt tattcaaatt ttcatatcgc
     9781 gtaatttgtt gacttaatga tctgatatct tcttcaagct ctgattcttt taaagatatg
     9841 ctatcaacct cactcgtata acgtgacaca aaattatcgc aagcttgctt cgttaaatca
     9901 ctcaatgttt tcatacttgt tgataatgga attaacaccg tactaaaaaa ttgcttagct
     9961 gacgtatacg ctttaccttt aagcgcatca tcattgataa attgagtaat tgctttttcc
    10021 aacgcatcat aatttgaatt cattgtttga ctcaaattac ccacacttga agcttggttt
    10081 cgagatctgt ctaaatacat gtcaatactc atcggcatgc tcctttttca aaaatatatg
    10141 attttcaaac tatttaaaat caaatgcttt ttacatctac aaaattgtaa aattttaaaa
    10201 ctcggcgatg attatttctt atgtaaagga gtctagatgc aggtaaattg agataacatg
    10261 tcgccttttt tcttatttta gcatatggat ataatggtgt ctttgtatat tcgcaattaa
    10321 taaataaaaa ttatctttca atattttaat tttattgcga caacatcctt aacattaaat
    10381 atattaatat ctcaaaatat attcactatt aaaatatgtc atcagttgtt aaaagtattt
    10441 cctcattatg cgaaatatca aaacgtctct aaaatacgaa caagtttata caatcacaca
    10501 acatcatcat tcaaaatttt attgccctta ttttcaatac tcatttcata tctattagtt
    10561 catacataac taaaattcaa ttacgcaatc tcaccgttaa tgcaacaact ttcaaataat
    10621 aaatcatcat aataatcaca cctaattcaa cacttttcaa acataagtat tgacacattg
    10681 agcaaaatga tttttaattg taactaatac agttacaatt acgaggtgag aaccattgaa
    10741 tttagaattt aacattgccg tacatgtatt aactttttta actaagcatc attcagaaaa
    10801 attcaatagt agttcattag ctgaattaac ttgtttaaat cctgttcaat tacgccgcgt
    10861 gacgactcaa cttgtcgatt taaaaatgat taacacaata cggggtaaag atggtggtta
    10921 tttagcaaat gatcgtagcg ctgatgtctc attagcaaca ttatataaac attttgtctt
    10981 agagaaagaa catcacacac gactatttac tggcgacgaa ggtagtcact gtcaaattgc
    11041 tcgtaatatt gcaactacca tgtcacatta tcaacaagac gaacagaata acattattaa
    11101 tttttataaa gaaaaaacaa tcaaagatgt cattgaagac attcaaaagg aggatttatg
    11161 tcatgaaaac atatgattta attgtaatag gatttgggaa agctggtaaa actttagcga
    11221 aatacgctgc atcaacaggt caacacgtcg cagttatcga acaatcttcg aaaatgtatg
    11281 gcggcacttg tatcaacgta ggatgtatac cttcgaagac acttgtacat gatggattag
    11341 aaggcaaatc ctttgaagca agttataacc gtaaaaacga tgttgtcaat gcgctaaaca
    11401 ataaaaatta ccacttatta gcagacgaca gtaacattga tgtattggat tttaaagcgc
    11461 agtttaaatc taatactgaa gtgaatttat tagatcagca tggtgatatc gtcgatagta
    11521 ttactgcacc tcatatcatt attaataccg gtgctacctc tatcattcct aacataaaag
    11581 gccttgatca agctaaacac gtcttcgatt caacaggttt attaaacatt agctatcaac
    11641 ctaagcacct cgttattgta ggtggcggtt atatcgcctt agaatttgct tcaatgtttg
    11701 cgaatttagg tagtaaggtt acagtattag aacgtggtga atcatttatg ccacgcgaag
    11761 atcaagatgt cgttgcacat gctattactg acttagaaaa taaaggcatt gcattgcata
    11821 caaatgttga aacgactgaa ttgtcatctg acaattatca tacaactgtc catactaacg
    11881 ttggtaactt tgaggctgat gcagtacttt tggctatcgg acgcaaaccg aatacggatt
    11941 tagcattaga aaatactgat atcgaattag gcgacagagg cgaaattaaa gtcaatgctc
    12001 atcttcaaac aactgtgccg catatttatg ctgcaggtga tgttaaaggc ggacttcaat
    12061 ttacgtatat atctttagat gattatcgaa ttatcaaatc atctttatat ggtaatcagt
    12121 cacgtacgac tgacaataga ggcagcgtgc cttatacagt atttatagat ccaccattat
    12181 cacgtgttgg attaactagc aaagaagctg ccgctcaaca ttatgattac actgagcatc
    12241 aacttttagt aagtgctata cctcgtcaca aaattaacaa tgatccaaga ggtttattta
    12301 aagtagtcat taataatgaa aataatatga ttttaggtgc tacattatat ggtaagcaat
    12361 ctgaagaatt aattaatata attaaacttg cgattgatca aaacattcca tataccatat
    12421 tacgagataa tatttatacg catcctacga tggccgaatc atttaatgat ttatttaatt
    12481 tctagacaaa acataaaaac ctggtggcac gcattgaatg atgctgccat caggctttat
    12541 tgttgtgctt ttcgcttttc taatttttct ttaagctttc tatcttgttc ttctttacga
    12601 cgtttacgtt cttcatgtcg ttttcttaaa cgctcttctt cttcaggatc acgtggtttc
    12661 tttaattgtt gagaaacttt ttcgattaat tcttcttcag taagcgcagc cagtgggcgg
    12721 ttattaacaa aagcgaatgt ttttcggcgt ccaggtccac aataagattg acaacctatc
    12781 acgatttcag catcgggatc taatttttcc aacttctttt gtaacgttct tatattgact
    12841 gcctgacatt catcacaaat aaggaatgta tttttcatat tgctacccac ctttctttat
    12901 catatctata tcgtcgattt cattaatttt ttcgttaact ctatctattt tactcttttt
    12961 aatatttttt tcaagatacg taacacagct ggcaataaaa aatggagcat ttatcttcta
    13021 attaaattag atgattgctc ccctatcaaa tcatttattg cccatgataa atattaaatt
    13081 ttaatggttt aataccatgt tttgtccatt catcataaat tttttcttta tctacatctt
    13141 tattgataat tgtaatacca cagtctccac caccagcgcc tgatgtttta gatgcgccgt
    13201 gatacttttc agcaatatca cacaaatatt ttagcttttc agtttctata tcaactgtag
    13261 cttctttatc catacgttga ataattgtac gattctgacg caccatcttt tgcacacctt
    13321 taatgttatt tgttttaaaa gcatgaataa gtttttcaac acaacgatgt gaatcttcta
    13381 agaagtcacc gtaaaatgaa ggatctgatt tcaaacgttt cacttcgcta acaaagtgtg
    13441 gtgatgacgc cggtgagcca gtccaaccga taagtacttc catattttca ggtgcttgta
    13501 atggttcgat gtgcaatcca ggccagtttt tgattaaaac ttcttcaacc gtagtatctt
    13561 caatttgatg cttaacccat tcatgatcaa aagtactata cgctagccat ccactatata
    13621 cactcacagc aatatctccg catgaactta aactttgtaa cttcatattt gcaatcactg
    13681 ctagtttata aatgtataaa ttagataact tcatatcata aaattcattt aatactttta
    13741 taactgatac aagtactgct gcacttgaac ctaatccata tttatgacca tttgaatcat
    13801 ctaaattact atcaatagtc agatgaaaat gcttcatcgc tatatcgcag cttttcgcat
    13861 actgttcaaa tatttcaata gctgtgacca cataatttaa ttgttttgct gcatgtggat
    13921 ctgaaatgac aatactatct tcatctctac taaatgtaac tgggttatga tgtaatgctt
    13981 ttgaatgaat ggtaccttta tattggtctg cttcttcaat agtagcagtt acaaaacgat
    14041 ctaacgcaat aagtacagat ttatatcctg gttctgttac agcatattct ccagcaatat
    14101 aaagttttcc gggtgctttg acctgaatca ttttatctct tccttactca attatttcaa
    14161 ttcctgtggc aataatgtca ctatcaataa tttggttatt atcaaactgt gttaataatt
    14221 tatctataat ctgttgcttg tttttctttt ctacaagtat tttcacatta ggtcccgcat
    14281 ccattgtaaa ataacacgga taccccgctt ctcggcattc gtgaacgagc gccattacat
    14341 cataactttc ttgcacaaga tatgtgaacg gcggtgttga tcctagattc gtggcatgca
    14401 tacgcaaacc attttcttca attacttcac caaggcgttt aaaatctttg tcttgaatcg
    14461 ctgcttttgc ttcagctaaa tcttcatcaa tatgatctaa ccaatattga taaaatcttg
    14521 atgtgtttcg tgtcaacgac ataccatatc gactaggtac ctttttagaa tgttgattaa
    14581 tcacaacaaa tatcatggca aggtcatctt caaaatgatt cgattcaagt ggaacggcat
    14641 atgacgtctc atcattatac cctttttccc attctgcaaa tccaccataa atactacgcg
    14701 acgcagaacc cgaaccgatt cgcgccaatc tcgataaatc cttatctgac agctgcaagt
    14761 ctagcgcttg attacaagct gctgctaaag ctgcatatgc gcttgctgat gaagccaatc
    14821 ctgctgctgt tggtacaaaa ttgtcgcttt caatttctgc ataccaatcg atgccagctc
    14881 tatttctgac aatatccata tattttgaaa ttttctctaa ttctttgcca ctaacctttt
    14941 caccattcaa ccaaaattga tcctgtgtta actggtcgtt aaaagtgact ttcgtttcag
    15001 tgtaaaattt ttctaatgta acagatatgc tattattcat tggaatgatt agtgcttcat
    15061 cttttttacc ccaatattta ataagtgcaa tattcgtatg tgcacgtgct ttgccacttt
    15121 taatcaacgc attaacctcc taaattctca atccatgtat gtgctgcacc agctttttct
    15181 acagctttta caatattttt cgctgttggt aaatctttgg caagcaataa catacttcca
    15241 ccacgaccag caccagtaag ttttccagca atcgcaccat tttctttacc aattttcatt
    15301 aattgttcta ttttatcatg actaactgtc aacgccttta aatccgcatg acattcatta
    15361 aaaatatccg ctaaggcttc aaatttatga tgttcaatca catcactcgc acgtaaaact
    15421 aacttaccga tatgttttac atgtgacatg tactgagggt cctcacaaag tttatgaaca
    15481 tcttctactg cttgtcttgt tgaacctttc acaccagtat caataacaac catatagccg
    15541 tctaaactta acgttttcaa cgtttcagca tgaccttttt ggaaccaaac tggtttgcct
    15601 gatacaatcg tttgcgtatc aataccactt ggtttaccat gtgcaatttg ctctgcccaa
    15661 ttagcctttt caatgagttc ttctttcgtt aatgatttcc ctaaaaaatc ataactcgca
    15721 cgaacaaaag caaccgcgac agctgcactc gatcctaacc cacgtgatgg tggtaaattc
    15781 gtttggatcg ttactgctag cggctctgta atattattta attctacaaa acggttcacc
    15841 aaagacttaa gatggtcagg cgcatcatat aacataccat cgtaaacatc gcttttaata
    15901 gacgaatagt tcccgctctc taaggcttct attaaaactt tgattttacc tgcgttaaac
    15961 ggtactgcaa tagcaggctc tccaaatgta acagcatgtt ctcctattaa aataatctta
    16021 cctgtcgatt ccccatatcc ttttcttgtc atgtcaatat caccttttat atttatccta
    16081 tacttgatac attgttttta tttattagta aaagacatca tattctaagt tgcatacgca
    16141 ttcgcgttaa atttcattgc agtctttatc tcacattatt catattatgt ataaacttta
    16201 ttttaaattt atatatgact taacttgatt agtataaaac taactttcgt ttacttcaaa
    16261 gtttaaatct tatcgagtga tatttcagat tctttatctt tttataaaat agccctacaa
    16321 tttataattt tccaccctaa ctataatact acaaataata attggaatat atagatttac
    16381 tactaaagta ttagaacatt tcaatagaag gtcgtttctt tcatagtcat acgcattata
    16441 tataccctat tctcaatcta tttaatacgt aaaacatgaa atttccttat taaatttatt
    16501 atttcaatca tatcattact tttaatttaa tgatgttcaa tttaaatatt aggtcaataa
    16561 catatttatg ctatttatgg atactttcaa aaataacagc cccaaacgat aacttgaaag
    16621 gggctgttaa atatttaact attgcatttg gtctatcatt ttcttgtttc tttcaatcat
    16681 tttatcaaaa tacgtatcgt atctttgcca ttcttcttga gtaattggcg tcatatttaa
    16741 tacaccgcca agatctttga ttgcatataa tagtaaaaac attacatctt gaacagtaat
    16801 cgttttgtta agtaatgtct ctagtgaggc catacaagat ggttcaattt cagggtattt
    16861 aaatttagtc acttcacctt ttaaagcatg ttcataaaat gtttgcatca tcaatgcacg
    16921 ttctgaacca gagccttcaa cacaaagata aatttgtaca gcaataccgc ctctaactct
    16981 tcgttgcgat atgcctgcaa atttcttacc atcgatactt aagtcaaatt ttcctgggca
    17041 ataagaatgt tcaatttcca tcgtatcaat atcaaaattc tcatcttcga acattttgct
    17101 aattaagaga tacatcacag taaacgcttc atcaatcgtt gtttctgttt gtcctttgaa
    17161 catcagcgat atatttaata caccttgatc tagaacgaca cctaggccgc cagaatttct
    17221 aacaatggca ttataaccaa tctcattcgt taaataatca atgccatctt ttaaaaacgg
    17281 caatcttgaa tcatgaatac caagaataac agtatgttga tgaatccaag tacgcacaac
    17341 attatctgat atatctttgc ccacactttc acaaaatgta tcatcgaatg cgaaagattg
    17401 cataggctct aatccagaag aatgatcgat atatcgccag ttgacgccat taaaatattt
    17461 actcgctaaa tccatcgtta ttgtaaggct tgcgctgctg tgatgaatga aagattgtat
    17521 acatcttcaa ttgagcagcc acgtgataag tcatttactg gagaatttaa accttgtaat
    17581 actggaccaa ctgcatcata tccacctaaa cgttgtgcaa ttttgtaacc aatattacca
    17641 gcttctaaac ttgggaatac aaagacattt gcatcacctt gtaatttagc acctggcgct
    17701 tttttctcag caacacctgg tacaatcgca gcatcaaatt ggaattcgcc atcaatgatt
    17761 gcttctaatt tttcttcttc agctttttgt tgtgctaatt tgacagcttc ttgaactttt
    17821 gtcacatcgt ctgatttagc agaccctttt gttgaaaagc ttaacattgc aacttttgga
    17881 tccatgccaa agcttaatgc tgattttgca ctttctactg caatttctgc aagtccttgt
    17941 gaatcaagtt ctggattgat tgcacaatca ccaaagatgt actgttcatc acctttaatc
    18001 ataaagaaga tacctgatgt tcttgataca cctggtttcg ttttgatgat ttgtaaagct
    18061 ggacgcacag tgtcgcctgt tgaatgtgct gcaccactaa ctaaaccatc tgctttacca
    18121 gcataaacaa gcattgtacc gaagtagttc acattgttta ataattcttg tgcttgttct
    18181 tcagtcgctt tacctttacg tcgttcaaca aatgattgaa ctaattcagc tttcaattca
    18241 cttgtcgcag gattaattaa ttcaatatta gaaatatcaa gattaagttt ttgcgctaaa
    18301 gattgaacct tagtctcatc acctaacacg attggtgtaa cataatctgt tgcttgtaat
    18361 tgtgttgcag ctgttagaac acgctcgtcc tctccttcag gtaatacgat tttaacgttt
    18421 ttaccagaaa gtttgtcttt taatacattt aataaatcag ccataatgtc ctcctgtaat
    18481 ataaatctta ttaatcattc acggtataat tatacgccat tttatttcat aatgccacgt
    18541 caccaatttg taatcataat tttatcgtta cccctttgct ttatgataaa atttataaag
    18601 aactgatgat ttttgaaaag gagcgataaa catgagtcaa gcagccgaaa cattagatgg
    18661 ttggtatagt ctacatttat tttatgcagt tgattgggca tcattacgtc tagttccaaa
    18721 ggacgaacgc gatgcacttg tcactgaatt tcaatcattt ttagaaaata cagcaactgt
    18781 aagatcatca aaatctggtg atcaagctat ttataatata actggtcaaa aagcagattt
    18841 gttattatgg ttcttacgtc ctgaaatgaa gtctttaaat catattgaaa atgaatttaa
    18901 caaattgcgc attgctgact tcttaatccc tacatattca tatgtatcag tcattgaatt
    18961 gagcaattat ttagctggta aatctgatga agatccttat gagaaccctc atatcaaagc
    19021 aagattatac ccagaattac cacattctga ttatatttgt ttctatccaa tgaacaaacg
    19081 tcgtaatgaa acttataact ggtacatgtt aactatggaa gaacgccaaa aattaatgta
    19141 tgaccatggt atgattggta gaaaatatgc tggcaaaatc aaacaattta ttactggttc
    19201 tgtaggtttt gatgatttcg aatggggcgt aacattgttc tcagacgacg tattacaatt
    19261 caaaaaaatt gtatacgaaa tgcgctttga tgaaacaaca gcacgatacg gtgaattcgg
    19321 tagtttcttt gtaggacata ttattaacac aaacgaattc aatcaattct ttgcgatttc
    19381 ttaatacatt ggtacgttta taaattaata aaaaattcca agcttatcgg tttaagcttg
    19441 gaatttttcg tttatcttca gtatattccc gtatacataa gacgtgattt ggtaaatagt
    19501 tgaaatctgt atgtttaaac ttatatatat gtgctaatgt aatatcaata acaaagtaca
    19561 ctttgctcat agcaaagtgt acccgagtag tcttccttgg gagaacttta actactatca
    19621 ctacatataa acgttaacct caatagaaat tatacagtcg ctactctata caatttttgt
    19681 aatggttaac taatattatt ttaacctatt tgaaatattt gaaacatatt tttgtcgaat
    19741 ttttttcaat attttttcct ttttatactt caagagaatt ttaactacta aaaattccga
    19801 tgattattat tacaatagta tcaaatatta gttttttaaa atcaataaca acttatcaaa
    19861 aagctcatgt ggttatttta tagtgtataa actataatga gtattaaatt cttataaaca
    19921 atggtgatga aatggacata aattcagaag aatacaaaca agaggtactt atcaaagatg
    19981 ttgtcatgct tgctgctcgc atactattag aatctggtgc agaaggtacg cgtgtagaag
    20041 ataccatgac acgtattgca aaaaaacttg gttacagtga aagtaacagc tttgttacaa
    20101 acactgtcat ccagtttacg ttacattcgg aatcgtttcc tagaatattt agaattacct
    20161 ctcgagatac aaacttaata aaaatttctc aagctaataa aatttcgcgt caaattacaa
    20221 ataatgaaat ttctttagcc gaggcaaaaa cacaacttga aaaaatatat gttgccaagc
    20281 gtgacagcag tcttcccttt aaaggttttg ctgcagcaat gattgcaatg agtttcttat
    20341 atttacaagg tggtagattg attgatgttt taactgcgat attagcaggt agtctaggat
    20401 acctagtcac tgagatttta gatcgtaagt tacacgcaca gtttatccca gaattcattg
    20461 gttcattagt tattgggatt atcgccgtta ttggacatac acttattcca acaggtgact
    20521 tggcaagtat tatcattgcg gcagtcatgc ctattgttcc tggtgtatta ataacaaacg
    20581 caatacaaga tttatttggt gggcacatgt tgatgttcac aacgaaatca ttagaagcat
    20641 tggttactgc gtttggcatc ggtgctggcg ttggtagcgt attaatttta gtataggagt
    20701 atcagactat gttttggata ttaaacttta tctttagctt tttagcttca atgttcttct
    20761 gcgtcatttt tgatgcacct agaaaattat atttatcatg tggtttcgtt ggtacgtgtg
    20821 gatggatggt ttacacctta ttcttcaacg gctttaatgt gcacactata tactctagtt
    20881 tcttcggcag tcttgcccta ggtttattaa gtcactatat ggctcgtaaa caaaaagaac
    20941 ctgcaatcat ttttatggtg acaggtatca tcccgttagt gccaggtgga ttagcttacg
    21001 atgcaacgaa aaatttagta ttattaaatt tcagtacagc aatcaatacc atgctagagg
    21061 ttacacttat tgcaggagct atcgcattag gtttattatt cgccgaccaa atttccaaat
    21121 taattgtttc tggattcgtg aaatctttta aacgattata aatatttgtt agaaatgcct
    21181 cagccttaac tcttggcaat tttatataag gttcaactgc ctcgtcttaa atgtcaattt
    21241 atgatacaaa aaatcgcacg atacgccttc aaattaagaa ggtatattgt gcgattcttt
    21301 tattatttag ttttttcttt ttcaggttct tcaatcatat catcaacttc attttcactt
    21361 ctatttaaaa tcaagcgact taattcatca ttatccatat ttttcaattt aggatatcga
    21421 atcacaaaga atattaaacc gatgattaac catccaagta atgcaatata agatggtgca
    21481 gtcagtgctg caggagaacc tggcactaat aacaacgcta agaaaatgaa tgatacaaat
    21541 gagccaataa tagcaaacgt tttgtaaacc ggtgcatacg tattactttg tttgttataa
    21601 ctgaataatt tcgcagcaga caaacatgta ataaagtagg caatggatac acctgtagat
    21661 gacatatcta cgatccaagt caatgccgtt cttcctagcc aaggtgcaat taaagacaca
    21721 cttactaaga atatgattgc gacatatggt gttttgtatt tactatgtaa tttactaaac
    21781 attgttggca ttatacctga acgtcccata gaaaataaca agcgacttga actcatcaag
    21841 aatccattta aaccagtaaa tatacccatc ataattgcaa ttgctaatac acctaatcca
    21901 atataaccaa atgctgtttg tgtaacagca cctgttaacc acaattgtcc atttaaactt
    21961 tgatgacttg ttaataacca accagtgtat aaaatcatga caacataagt taatgatgct
    22021 gctaataaac tgtacacgat aagcttaaat gtcttgtttg gtgcaaagtt aaactcttct
    22081 gctgtttgtg gaatattatc aaatccaaca tatgcccatg gtgccacgga tacaataacc
    22141 acaatagaca ctaaccatcc tttgctaggt tcagctaacg gttgtaaatt ttcaagtgca
    22201 aaattattac caaagaatga accaaagaac atcaataata cgacgattac catcgccaca
    22261 cagaaataat attgtaatga tccagataca cttgcgccac gaatcgttac tagcatgaat
    22321 acaagtagta atacggtcgc aataatgatt tccgtaatat aaacgtccca gcccgcaatg
    22381 gtgtatagtt tcccattatt taagacatct ggcaataaga atttaactag taaactgaat
    22441 gctgtcgcat ttaaagcaac aacacagaca taaccaaaag ttaaaaacca tgatgagaag
    22501 aaactcacat atctgccgaa acttaagaaa ctaaaggcaa acgcgccccc tgatactgga
    22561 aatctctcta ctaatgcgcc ataactaacc gcaattaata tcattaataa tgcaccaata
    22621 acaataccaa ttgatgctgc aatcggacct gactgcttaa tccagtctcc tggtaagatg
    22681 aatgcgcccc atccgataca tgaaccatat gcaatcgccc atacaaactt ttcagatagg
    22741 ttttgtttta aatcgcctct atctatttgc ttattctttt tttccataaa taaaaactca
    22801 cctcgaaggt attctatacc caaaagatga gtttgtaact ctttttcttg attattttta
    22861 ttaaaaattt aatgtttagt tacataggta atctaaagtt ttaaaattta accagcaaat
    22921 ttgaatgtcg caatgattaa cattatccat ccaatgatga acaatacgcc accaattggc
    22981 gtaatcgcac ctaaaacttt aatttgagtt aatactaaaa tatataatga tccactaaag
    23041 aaaataatac cagcaaatat taaccagcca gcccagttaa cattgattga agttgtacca
    23101 ctaattacac ctataattaa taatgctaag ccatggtaca tttgatacgt cgttgctttt
    23161 tcccatactg ataaatagtg atcacttatt tttccttgta aaccatgtgc accaaatgca
    23221 cctgttccga cagccatcat cgcgtttaat gcacctaaaa taataaataa tttcattgct
    23281 acctctcctt atataattca gtattaataa atgtttaaat ccgaacactc gaataaaagt
    23341 atatgcatct tgattattaa cctacatact taacaagcta ttaagtaatt atgtttcatt
    23401 gttgcttttt aaaaatcaaa tatggattca ccattgccaa tgtcatcatc tgtcgctaat
    23461 ctttgttgtt ggtttgaagg ttgtgaatat gacttattat gatgatgtgc tgaatgcgta
    23521 tttactttac cacccatcgc ttcaatttca gcagctgtca cttgatgtgt tgactgcgta
    23581 atttgtgaat gtggcatttg attatggtta gcaatacgat gattcatttg ttgttcacca
    23641 atatgtggtg tatttgatgt tggagcatat aaagatgtta atatatgtat ggcatacata
    23701 tgtttttcaa agtcatggtc atgctgcgcc tgctctgctt gtactaattc ttgctcaatt
    23761 cgtgctatta aagtttctct attcaccatc tacgcctcac tttcacacca attaattggt
    23821 gattttccta ctgactctaa ataggtattc gctttggaat acggttttga tccaaagaat
    23881 cctctatatg cagacagtgg actaggatgc actgatttta taatacaatg tttagatgta
    23941 tcgataagct ttattttttg ctgtgcaggt ttcccccaca aaataaagac aacatgttct
    24001 ttataatcag acactgcttt aataatttca tcagtaaatg tttcccaacc aatatcacga
    24061 tgagaatttg cttcaccctg tcttacggtt aaaactgtat tcaataacaa gacaccttct
    24121 cttgcccaat cttgtaaatg tggtgtttgt ctaacacatc caatatcatc tgctaattct
    24181 ttatacatat tacgtaaaga tggagggaat tttgcgttag gttgcactga aaatgctaat
    24241 ccatgtgctt ggttcggacc atgatacggg tcttgtccta atataacaac tttgatattt
    24301 tcaaacggtg ttaaatcaaa cgcttgataa atattttccc tatcagggta tacgattgca
    24361 gtcgaatatt ctttttctaa aaaatcatgc atagctttaa agtcatgttt cgttgttatg
    24421 tcatgaaaaa tttgcgacca ttccattcaa tctcacctca ggacatattt taacatatca
    24481 cacttatcta aagtgaatga cattttcaaa agttacaata gtatgaatga ttcatttcaa
    24541 caatcatcaa atattgaatg cttaatattt ttcaattagg ttaaaaatga tatgatgaat
    24601 acaatattat tatgtaggag tgaagctcaa tggctttaaa gaaagtttta acaattgccg
    24661 gttctgacac aagtgctggc gcaggtatgc aagcagattt gaaaacgttc caagaattag
    24721 atacgtatgg catggtcgct ttaactgcca tcgttactat ggataaagat acatggtcac
    24781 acgatgtcac accattacct atggacgtat ttgaaaagca acttgaaacg gctttatcaa
    24841 tcggacctga tgctattaaa acaggtatgt taggtactga ggagataatc aaacgtgctg
    24901 gagaagtata tgaggcatct aatgcacaat attttgtagt agatcctgtc atggtttgta
    24961 aaggcgaaga tgaagtgctt aaccctggaa atactgaggc gatgattaaa tatttactac
    25021 ctaaagcaac ggtagtgacg ccaaacttat ttgaagcggg tcaattatca ggattaggta
    25081 aattaaattc aattgaagat atgaaaaagg ctgcaacaat tatctttgat aaaggcgcac
    25141 agcatgtcat cattaaaggt ggcaaagctt tagatcaaga taaatcgtat gacttatact
    25201 atgatggtca aacattttat caactaacta cagacatgtt ccaacaaagt tataaccatg
    25261 gtgctggttg tacatttgct gcggcaacaa ctgcatattt agctaacggt aagtcaccga
    25321 aagaagctgt gattagcgcg aaagctttcg tcgcttctgc tattaaaaac ggttggaaaa
    25381 tgaatgattt tgttggtcct gtggatcacg gtgcatacaa ccgtattgaa catatcgatg
    25441 ttgaagtaac agaggtttaa tgcttcgttc ataaaaaata gattaaccac accttatgat
    25501 ttgattgtca ttgctgtgcg cctgtaaagt tagctttttg agtctaactt tggggctcag
    25561 tacacaataa agtcactttc ggtgtggtta ttttagttat taaatacttg tttgattttc
    25621 acagttaaat tgtgcaaata agttttgtaa gaaatcgtta tagatagttt tatttcaaca
    25681 tatgtatata atattcattt acagtgtcac gaatcagtta tcctaaaaaa acaaatcctt
    25741 tcggaacttt attatgccct tgtttaaaca taatgtttta tcccgctcta ctaattctgc
    25801 cattttttga aattaaaaat aacttctcaa cgactttata aaaatatatt ccttttaaaa
    25861 catgatacat aaatagtctt agtaaattat acgttctatg tctgataaaa agttgatact
    25921 taaggttgat gtcacacata aaaatgttat ttataatata accatagagt agcaaaggag
    25981 gtaatatagg ttatgattat ttatcgacag tatcaacatg aaggcgcacc agtttatgaa
    26041 attataacca aaacgtttca gcatgtttca attaaatgtg acgattcatt tagtgatact
    26101 gaaatattca aattgctctc tttattacaa gacgatatag atcatatgaa agttagttaa
    26161 attgactaat cgttccaata atccagaatt tattaaacat tgatatagtt gttattatta
    26221 ttttagatta atgcatcccc gtgcaaagaa agaaacgtaa cctatattta tctctgttcc
    26281 caataataat aacaatacat aactgatatt agctgtcctt tcatctccta attaatatgc
    26341 gttcatgcag ctaatatcgg ttattttttt gtgctcaaag aaatctttat tttaatctct
    26401 ccatcgtcat taattgctat aatttcttgc tcgtgttctt caatgaattt gatattttca
    26461 tttattaaaa taggtttaac atagttaatt tgacactggt tcgcctctaa tgacatagcc
    26521 atcaacatca tttgactcac taattgtcct ggcacaatct cattatgtat tggattatca
    26581 tcatttacga gcgccaaata ttcatttatc catatctcat taaacttcat ctctacttca
    26641 tcgccttaat aaaagtttgt gtaaccgtta tgacatgttg atcattttta gttaatgtta
    26701 aagccattgt ataacgtgtg atattgcgaa tctgtcggca ttcaatatct tctaaatgtc
    26761 ctacatatat tgtgtctact tttatctttt gagtttgatt aattactgat tttgttaaaa
    26821 tcagttcgct atttgcatat tttttaaata aatcaaattt tgaccataat gacgcacaca
    26881 ttaatggtgg tacttcttta ctataaggtt ggttaaaaca atgataatat gcagccacac
    26941 tatccttaga aaatgacaca tttaatcgct ggttagaatc gagtaaataa tgctgcattt
    27001 cctagacctc ccccaatccc catagatgca atcatactct ctttgtcaaa catataaaat
    27061 aatcgcgtca ctaattgggc accgcttgca ccgtatggat gacctgatgc taatgcacca
    27121 ccccatatat ttaattgcgt atttgaaata tttaaagctt gttggcaggc aaccacctgt
    27181 gcactgaacg cttcgttaat ttcaatgact tcaatatttt ctatcgttaa ttgatttctt
    27241 tttagtaagt tggaaatggc tggtactgga ccaatgccag gtaaattaga atcaacacct
    27301 accgtaacac catctttaaa taataaacca tgatcgaaac ctaattcgta tgccatatct
    27361 ttttccataa taagcaatag aactgcacca tcatttttca tacaactatt cgcagcggta
    27421 acggtcccac ctttgattac gggcttaaat cggccaaagt tatctttcgg aatatgtgat
    27481 tttagacttt catcagtgtt gaatagttct cctttaacgg ttataggtaa tatttcctga
    27541 gaaatatttc cattctttac attttccgct gtcagttgat gacttcggta agcaaattca
    27601 tcttgtaatt ctcttgaaac accatacatc ttggctacat tttcagcacc ttgaatcatt
    27661 gatgggtcgc tcatttcagg tgcaaatgat gcacgctcat aaaactcagg taatgcggtt
    27721 tcgtacacag aatgcggtcg tttgattttc caaggtgctc gacttgtact ttcaacaccg
    27781 cctgcaatat ataccttgcc agctccggct tgaatcatgc gacatgcata ttgaacactt
    27841 tcaagtccag acccacattg ccgatcgatt gtgacgccag gtattgaatc tttgagcccc
    27901 gcttcaagca atgcttttct tgcaatattg ccaccattcc caacaacatt acctaaaact
    27961 acatcatcta ttttagatat tacctctgga tacttctctt taaaatgttg gaataaaggt
    28021 ttgagcaatt gttctggctc taaatgtttt aaagtgccac catatttccc aaatgcagtt
    28081 cgtttagctg caactatgac tgcttgattc atattataat tcacctctca aatacatcga
    28141 catcattttt tctctagcaa ttttaccact tgcagtgtaa tacatctttt ctacatgatg
    28201 aatcatcgat ggaatttcat agcgtttcac tttcttattt aaaaattgtt ttacattttt
    28261 atgtgtaagt gtcacatcac cagaataaag caatacacct atttgaccaa aacgctcatt
    28321 tggaataccg atgataattg cttcatcaat gctcgacgat tgcgttaaaa ggcgttcaac
    28381 atgtgctgga tatatatttt gaccaccaat aattaacata tcatgttgtc gtcccgttaa
    28441 atataaatac tgctctttta catagccatt atcattagta acaaaccatt catcattatt
    28501 tatacattgt tcgcttacat agccactaaa catcatatta ctttttacac aaatagttcc
    28561 tataccattg tgatcgtgat tcgttgtttt caattccaca tttggaaata gcacacctac
    28621 agattcaact ggtgcttgct gattcaagtt atagctgata aaactggttt ccgatgtacc
    28681 aaaaaattca atcaaattta tgtcatttgc ttgatttttt atctttttaa aaatagaaga
    28741 atgcagctta tccccactgc taaaaaatga ttggattgta tgttcattgt tataaactaa
    28801 caataatgat ttaatcatcg ttggaacaag aaacatagca actttgtatg atgatatttt
    28861 acgacattga ttaagtaacc tttcaggatg aaaagtggtc tgtcctataa aagtacgacc
    28921 ggaacttaaa gcaaacaata acgcatataa tgttaacgag tgcgatagtg gtccgggggc
    28981 tgctattgca ttttcatttt ttaacatcaa catttcatta acttcaaaag aagccaacca
    29041 tgaatcttca tcacgataat atgcttttgg cagtccagtt gtccctgaag taaaaccaat
    29101 atgtaataaa ttggggtagt gctgtaattg cgttgaatca ataaacatag gtgagtctat
    29161 attctgcatg agacatgtat ctccaattac atctttaata ccatattttt caatcaattg
    29221 acgatgtata gtactttgcc atttaccttc catcatcata ggcactttat gtaaacgatg
    29281 caaagctaaa taacaaataa tactttgaac cggtgatttc atattaatag caacaacagg
    29341 gttaagtgaa tatttctgca aagattcaac tgcgctagtg atgcgggcat ttagttgact
    29401 atatgtaatt gtttcatcat ctatatgtaa tgctatgtca ttaggcttat tttgagtatg
    29461 tgtttccaac tgttctaaaa ttacgtccat caatatccct cactcaatgt aaactttata
    29521 atacatcttg ttaacattat ataatgaata agtattttta tcaggtaaac ctgcctatta
    29581 taaaaatcaa acttaagccc tagataaatt tgatactgta gtaccatgtt ttttattaga
    29641 taagacgata ctatatgtat atagagaaaa gaagttatcg caatcgcaat aactgacaca
    29701 aataagtcac ccaatctatt taagtttgtg ttctctataa taatattgaa ggttagagtc
    29761 tcaatattaa ttgcgttgga caaaatactc taagacatat ttacatccta acatttcaat
    29821 agcaagagaa ttttaagtat tcaaagctaa acatatgctt taagtcccct ctttccccca
    29881 ttgcgataat cattgcagtg ggggtttatg tatatacaaa aactttattt cggctaatta
    29941 cattccctaa tttaaaaagc actttaaagt tagtagcgtt tactagcttt aaagtgcttt
    30001 tatacgattg attattactt atttactgaa atctctaatc tttacgttct ttcacccacc
    30061 ataatgcttc ttttgggtgt tctacataat aagctctatc ttgctcgtta tctacatttg
    30121 gatatgaacc tttgagagat tttcctgctg tgctcaaatg taagaatgtg atcactaaaa
    30181 acccaataac actgattgct gttaaataat acgcaggtgc taatggatca ccagttttcg
    30241 cgactaacca tgttgcaact aatggcgtcg taccaccaaa tattgaaaca gagatattaa
    30301 aggttaccga taaagttcga tatcttatat gactgtaaaa cattgttggt aaagaccctg
    30361 gcattgtcgc ttcgtatgtt gataagaaga atcctaatcc aaatataccg atgactatca
    30421 caacaaatga ttgtgaatgt aataacataa atgcgatgat actaaataat gttaacccgc
    30481 cagtaccgat tagaaatact ttcttttcac ctattttatc cgctaactta ccaaacatta
    30541 atgctaatgg aatcattatt gccatgacac aagtaattaa tacactagtt gtcgttgcat
    30601 ctagtttaat aacttgttct aagtaagttg gtaagtatgc ggttaccata tagttcgtaa
    30661 cattgaagaa tacaacagct acaaaacaca caaatatatc tttgtaataa aatctgatga
    30721 tttgtaaaaa gttaatgttg tctcgttctg gttgtgttgc aacatcattt tcgaaaactg
    30781 gtgattcttc aagcttacga cgtaaatata agccgaataa tcctaggaat aaaccaagta
    30841 agaatgggat tctccaacca aatgatgcca tttgttcatc agttaagaag aaagtgagta
    30901 aggcaatcat aattgacgca gctatataac ctgataatgt ccctatttct aatccactac
    30961 ctaatgagtt acgacgctta tctggagatg attcggctac atatgtcatt gcccccgcat
    31021 actctccacc tgttgaaaat ccttgtagta ctcgtgcaag taataatagt attggtgccc
    31081 aaagtccaat ttgatcataa cttggtaata atccaatggt taatgttgaa aatgccatta
    31141 aaataattgt tgatgttaat acaactttac gtccatattt gtcaccaata ataccaaata
    31201 caacaccacc aattggtctt aataaaaacg caatggctaa tgctgcgaaa gtcaacattt
    31261 gtcgaatgtc tgcactctct actggagaga agaagttcgc tccaatgtac gcagttgtat
    31321 atgcatagac accaaaatcg aaccattcca ttgcattacc gataccggtt gcaacaacag
    31381 tctttttagc tttctttgca tccaccatgt taatattctc tttattaaaa tccatgaatt
    31441 catacactcc ctcttgtatg tttcataatt atacggagtt aattagtttt gtcaaattta
    31501 taagaattta aacttttata taaatacaaa agtatgtttg ttcaaaaatt tcactttatc
    31561 gaattaaatt caatgatttt ataccctcgt acatttgaat taaacaataa taattcttaa
    31621 atcatcgaac gattaacttt ttggaattat aactaaaaat agcgatagca tacaacttca
    31681 tataagtcat atgctatcgc cattttcgac atcgtattta aagtaagata ttcaattgac
    31741 tgcaagtaat atgtcatcag tcggagttat ttgtattaat atcaaataat taattacgca
    31801 gacttttcta tttaaacata cacgattatt aataaatatt tattttaaat tcctaatcta
    31861 aagcgaaata tttctatgta taatctaata tttataaata tggattttaa ccatggtatt
    31921 ctctgtttta acaaatttta taatacggta ttaatttctg atggatcatt gattataaaa
    31981 tctggatttg aatgaagtaa tgaccttgca tcatgtgctc cccatgttac tgctgcagat
    32041 ggtacaccag catgttgcgc catctcaaca tcaaatgttg aatcaccaat ataaaccgtt
    32101 tgttggctat ttaaattgta gcgttgcaca attgtgtgta tgccttcagg atttggttta
    32161 tatgcactta cttgatcgga tccaacagct tcagtgatta aatgatttaa ccctatagcc
    32221 gataaatttc tttctaatac atcactcttc ttactagaca ccacgaaaag ttttttccct
    32281 tggttataca aacttgtaat ggcttcagtt atacccgcaa attcataaat atagtccttt
    32341 tcaatagatt gatatgtatg tctaaatgta tcgattaact ttgctaatgt tgcttcatct
    32401 aatggtcggt ctgctaattt taaaaatgat tcttcaatag gtattcccat ataatgcgat
    32461 atgtcttgag ctgatggttc tgttaaacca catgctttaa atgcactttg tgttgctact
    32521 tcaccacatt ttttcgtgtc tgccaacgta ccatcaaaat caaaaatata attttcaaac
    32581 ttcaatatta acacccctat tcgtttatta ttgatattat actataattt attatttaaa
    32641 taaaaaaata atcgataata tttctgccta ggcatcatat tatcgactat ctttgtttta
    32701 ttctaaatta gcatgatttt gttgcatcgt ttgttctgaa acattcattt cagtcatcaa
    32761 tcccattaca acgctatcta aaaatagttg agatgcttgt tcaaacaaac ttcctaacgg
    32821 ttgtgcagaa ccatgttcat catattttgt acctgcaggc aaaacgatgt tcgtcccagc
    32881 tagattgcct attgcagaat ctttatttgt agtaattaat acgatgtcag cacctactga
    32941 ttttgcttta tctgctaata atcttaaatg ttccgtggaa cctgaaccag agataattac
    33001 aaatacatca ttcgacttaa tcgcaggtgt cgttgattct ccaacaacat gtacctgttt
    33061 gccgagctga tttaagcgca ttgcaaaact attcgccacg aatcctgaac gtcctttgcc
    33121 agctacaaat atatgttcag catgtagtat tttggacgca aacgttgaaa actcatccgc
    33181 ttcaacatgt gacaaagtca tctttaattc atctagaatt aattgatagt cactaaattt
    33241 agccatatta cttaccttcg attgcagcgc gacattgttt tgcagcttct actggatcat
    33301 ctgcatttgc gattccgcca ccaacaataa caagatcagg actttcagcg acaatttctt
    33361 taattgtatc tggtttaatt ccacctgcta ctgcaacttt agaattttta ataacagatt
    33421 taacggttct taaactttct aatggtgatt gcccttctgc ttgtaaatca taaccagtgt
    33481 gtactgcaat ataatcagca cccatttcat ctagttcttt tgcacgtttt tctaaatctt
    33541 gaacagcaat catatcaact agtaattgtt tattattttt atgagcttct tcaatagctg
    33601 ctttaattga tgcatcttct gcaacaccta gtattgtaat tacatccgcg ccaaatttaa
    33661 ttgcttggct aacttcataa tcagctgcat ccataatttt catgtctgct aatactttta
    33721 cattactaat gttgtctgcc atatgtttaa ctgctggtaa accttcgttg taaatgattg
    33781 gcgtaccgat ttctacgata tctacataat cttttacttt atttgctaac tcagccgcgt
    33841 cttctttgtt taataaatca attgctaatt gtaattccac taaaaatcat ccttccaatg
    33901 ctttttatct ttctattgga acctttcccg aggcatttct taattaaaca ttgcaatcaa
    33961 tccatttcat cgacatgaat tttcgcaacg tttttcgggc aagcttcgtc gtctaaatat
    34021 atctcaacat tcgggtgttt gtgaagtaat gtggctggaa catcaacaga aatttcctga
    34081 tttaataaat gtgtaatagc agcacgtttc ttttcaccaa atgcgagtaa aatgatacgt
    34141 ttagcttgaa gaatattagc aagtcccatc gaaatggctt gctttggaac atcatcttcg
    34201 ttttcaaaat atcgactatt agccttaata gtactttcag tcaaatcaac gatatgagta
    34261 acgctatcaa acggcgtacc aggttcatta aatccaatat gaccattttc accaatacct
    34321 aaaatttgaa tatcacgttg accttgttgt tctaaaacgt cattatatgt tgacgcttcc
    34381 gcattcatat cataggcatc tccattcgga atatgaatgt tctttctatt aaaataaggg
    34441 tattgtttga aaagcatgtc atccatataa tagtgataac tttgcggatg tgatgcggtt
    34501 aaacctacat attcgtctaa attaaacgtg gatacattgt ctacgtttaa ctgattttta
    34561 tttaataact taacaagttg ctcatacaaa tctgtcattg tgccgccagt tgctaaacca
    34621 agtttacagt gctgattaaa tgccatctct ttatataact cacatgcaac atagaatgat
    34681 gcttgttttt ttgaacctaa gtttaatact ttcattgttt acatgcctcc acaaatttaa
    34741 tacattttaa aaacttatga attcattcat acaccattaa tatagcatca tttttaatgt
    34801 ttaacacttt tcaaattcca acaatttttt tgatttttaa aagtaaaaaa ctaatgctgt
    34861 atgattgcgt aatcctgagt cattcagctt catgaaaatt aatttataaa taattgagca
    34921 atcgtataca attattgaat agtttttagt aaaatagaaa gataaacaca ttgatagtgt
    34981 aaggagtgaa cgtgttggaa ccaattaaag aacaagaagt tatagattta cttgcttctt
    35041 ttgaacataa acctgtgtat ctgcatgttg aaacgacaaa tggggcctat gcgaaccatt
    35101 tcgatcaacg tgtttttaat gctggtactt ttttaagaaa catacaagta acatatacac
    35161 atgcacaact aaaaggtggt aataaagaac cttatcgcat tggtcttaaa ctatcaaatg
    35221 gtggttgggt ttatgtacaa ggtctaacgc attttgaaat caatgagcat gatgaatttt
    35281 taattgcagg ttttaattac gaaggtcaac tcgctgcagc acttcaaatt agtgagcgac
    35341 catttaattt atagaggagg catcaacatg actgacgaaa gacatgtatt agtaatattt
    35401 ccacatcctg atgatgaaac cttctcatct gcaggcacat tagcaagtta tatccaaaaa
    35461 ggcacacctg taacatatgc atgtttaacc ctaggacaaa tgggcagaaa tttaggtaat
    35521 cccccatttg caacacgcga atcattacca agtattcgtg agcgtgaact agaagaagct
    35581 tgtaaagtta ttggtattac tgatttaaga aaaatgggtt tacgtgacaa aactgtcgaa
    35641 tttgaaccct atgaacatat agatggtatg attaaatctt taatcgatga tacaaatcca
    35701 tcattgatta tttcatttta tcctggatat gcagtacatc ctgaccacga agcaacagca
    35761 gacgctgtaa ttcgaacggt tgaacgcata cctaaagaag aacgccctcg tttaacttta
    35821 gttgcgttca gtaatgatgc aaccgaagca cttggagaac ctgatatcca aaatgatatt
    35881 acagatttta aagaacttaa aattaaggca tttgaagcac atgcttctca aacaggtcca
    35941 tttttaaaac aattagcaag tcctgaaatc gatggtaaac aacacagctt tttaacagtc
    36001 gaaccatatt ggacatatca ctttaaatct taacggagga aaaaaatgac tgaatttgat
    36061 ttatcgacac gagaaggtcg ttggaaacac tttggttccg tagatcctat tgaaggcacg
    36121 aaacctacta ctaagaacga aatgacagat ttacaaagta cacataaaga tttcttattt
    36181 gaaatagaag aagttggtat taaaaactta gtgtaccctg tacttgttga ccagtatcaa
    36241 acagctggca catttagttt ttcaacaagt ttaacaaaag acgaaaaagg tattaatatg
    36301 agccgtatta ttgaaagtgt tgaaaagcac tatgataatg gtattgaact tgaatttaat
    36361 acactttatc aggtgttatg cactttacaa acgaatatga aacaaaatgc ggcaggcgtt
    36421 gatgtttctg ggaaatggtt ctttgatcgc tatagtccaa ccacaaatat taaagcagtt
    36481 ggtaatgccg atgtcactta tggattagca attgacggtg ataaagttac acgtaaagaa
    36541 cttactattg aagcaactgt cacaacgcta tgcccttgtt caaaagaaat aagtgaatat
    36601 tcagcgcaca accaacgtgg tgttgtcact gttaaaacat atattaataa agatcaagac
    36661 atagttgatg actataaaaa caaaatttta gatgcaatgg aagctaatgc aagttcaatt
    36721 ctatacccta ttttaaaacg tcctgatgaa aaacgtgtga cagagcgtgc atatgaaaat
    36781 ccacgctttg tcgaagactt aa
//
LOCUS       DAMZPP010000007        36110 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMZPP010000007 DAMZPP010000000
VERSION     DAMZPP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 36110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36110
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.007"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(99..1091)
                     /locus_tag="POH73_000272"
     CDS             complement(99..1091)
                     /locus_tag="POH73_000272"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDI6886257.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     TMFDHVKKGAILVNAARGAVINTPDLIDAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            1361..2032
                     /locus_tag="POH73_000273"
     CDS             1361..2032
                     /locus_tag="POH73_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDI6886258.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            complement(2153..2959)
                     /locus_tag="POH73_000274"
     CDS             complement(2153..2959)
                     /locus_tag="POH73_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6886259.1"
                     /translation="MAFHDKTATQVTNIELNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNAQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(3134..3418)
                     /locus_tag="POH73_000275"
     CDS             complement(3134..3418)
                     /locus_tag="POH73_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6886260.1"
                     /translation="MSNLEIKQGENKFYIGDDENHALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLVKAVVEHARENHLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            3644..4078
                     /locus_tag="POH73_000276"
     CDS             3644..4078
                     /locus_tag="POH73_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6886261.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            4156..5124
                     /locus_tag="POH73_000277"
     CDS             4156..5124
                     /locus_tag="POH73_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDI6886262.1"
                     /translation="MTNNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALMYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNIALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEVRPFNTKRQHD"
     gene            5146..5739
                     /locus_tag="POH73_000278"
     CDS             5146..5739
                     /locus_tag="POH73_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6886263.1"
                     /translation="MEHIFREGQNGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPIEVRSTKKLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFKTRGAQVEEVWVNGHEITETGLTAGQQILEK"
     gene            complement(5844..6923)
                     /locus_tag="POH73_000279"
     CDS             complement(5844..6923)
                     /locus_tag="POH73_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886264.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMIVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVVFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHFDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(7266..9230)
                     /locus_tag="POH73_000280"
     CDS             complement(7266..9230)
                     /locus_tag="POH73_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDI6886265.1"
                     /translation="MTQITERELKKKYLDLLSQHFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKDKLSTKELNDLTALVYYPEDKLQL
                     IKCDFQNYGQLNVWYITTIEHLIQLIKYCSSKYTRSKLRRALPEQYVFIVEELLYKNN
                     EFKNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIVEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPVIKRRP
                     NFEMDERLVLEKVNYDTNEITVYGKTYPLKDTCFQTVNRDNPAKLLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPCNGNLLIHGCIPVDENGEMESFEIDGQTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRIVDKELQ
                     RKKIRNTNKGKELQAQIDILKMLMHDRYLD"
     gene            complement(9735..10505)
                     /locus_tag="POH73_000281"
     CDS             complement(9735..10505)
                     /locus_tag="POH73_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6886266.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            complement(10507..11202)
                     /locus_tag="POH73_000282"
     CDS             complement(10507..11202)
                     /locus_tag="POH73_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6886267.1"
                     /translation="MDVLTIEHLTKKIGNKTILDDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNEPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLDIIEREDQA"
     gene            complement(11888..13300)
                     /locus_tag="POH73_000283"
     CDS             complement(11888..13300)
                     /locus_tag="POH73_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDI6886268.1"
                     /translation="MTKFQYKKIIDDIINKINNGILSPGDKLYSQRKLAKYYNVNKST
                     VIQALDILKSYGILDTIEKKGIYVSQYKWNSYITNNIHWQDYIGNSFSKNNQYYIQKI
                     NEIEFNTDIVRLGTGELSPKLIPNHIFKKILTNDIEESLQTNYEEPKGNLKLRIEIVK
                     YMKRRGVDCNINNICITSGAVQGLKLIADGLLIPQSKIIIETPSYINSIRTWHNIRAK
                     IIPLSINYIKQNINNIFKLNSDYRHSIFYCIPTLHNPTQNTYSKEEKQKIIDQCHKEG
                     IPIVEDSIYSDLWFSPNQQISMKALKNSDNVLYLGSLSKTVSPGLRIGWIIADEKVIH
                     HLADLKMQNDYGASSISQYIATTWLSNYHEDHLYKLKIELKLRKSIFIKSLKKHLSKF
                     GYWNEPQGSFYIWFKLSVPVNIKLLFNEAIKENILIHPGEIYDINSKSYIRFSYSYID
                     KEEIDQSLKKLSEIINRIRT"
     gene            13399..14379
                     /locus_tag="POH73_000284"
     CDS             13399..14379
                     /locus_tag="POH73_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HDI6886269.1"
                     /translation="MTLKLEKAVFSMEIKKDLKLLMKKDNYHNIFALIFDWLVILGSA
                     CISIYMSNLIIYIISIILIGSRMRAFDNLMHEACHRSLFTNKFYNKWIACLLVAFPIF
                     TSFTTYCNSHFKHHRNLWDEEKDPDTKRYRIVGLDKPQKDVKKFIINHIVKVLFLFHV
                     PKYVLGTVSANLYSRDTPKSEIWTRNIFWVTIITLSIMFNFWLYVILYWFIPLLTTFQ
                     IIRYWAEMAEHSGLNNDSELTASRNTFGMPWTIFLFHPHHDNFHLVHHLFPAIPHYNL
                     KKAHIILMKDQKYREAHHCTGFFKTTLPGFYSVVKDICTPNIVGKGEDAN"
     gene            14376..14993
                     /locus_tag="POH73_000285"
     CDS             14376..14993
                     /locus_tag="POH73_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDI6886270.1"
                     /translation="MIQPVLHGILLALGLILPLGAQNVFVFNQGANQKKMINALPVVI
                     TAGLCDTFLIIIAVLGVSLILMSLPVLQLFIYIVGLFFLLYMAWSLWKEKPNTLENYE
                     PMSTKKQIGFAISVSLLNPHAIMDTVGVIGSSASVYSGLEKILFCIATIMVSWLWFFL
                     LAILGKVLGSIDRTGKYILFLNKISSIIIIAVSIVIVKNILKLIL"
     gene            complement(15224..15835)
                     /locus_tag="POH73_000286"
     CDS             complement(15224..15835)
                     /locus_tag="POH73_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI6886271.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            16386..17663
                     /locus_tag="POH73_000287"
     CDS             16386..17663
                     /locus_tag="POH73_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6886272.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            18140..19963
                     /locus_tag="POH73_000288"
     CDS             18140..19963
                     /locus_tag="POH73_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI6886273.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMILGTLTATTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGVLATMMVLITILLSKNKAEEQAIQNDFKLISKLLHAYN
                     DKSAFSQVAKTAVDSFKASDKLLITSNSSSDKLSGRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIITQRDDRNKNVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEAEMRKPLYSKRIYQNLTLDSIVFRNTLRYAAIMMIAIFIALMFDFEKAYW
                     IPLSAHTVLLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYTIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPYVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLATFENIAK
                     HLELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            20102..20518
                     /locus_tag="POH73_000289"
     CDS             20102..20518
                     /locus_tag="POH73_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDI6886274.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKPEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            20749..21441
                     /locus_tag="POH73_000290"
     CDS             20749..21441
                     /locus_tag="POH73_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDI6886275.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNKAETEKYRDLLKECATEITEVE
                     NKLQQIHSEITE"
     gene            21606..22337
                     /locus_tag="POH73_000291"
     CDS             21606..22337
                     /locus_tag="POH73_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI6886276.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLD
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSILSS
                     ILMKNKWPFLIALPFMIGYGIGITIYYQRKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKTHMGTE"
     gene            22524..23204
                     /locus_tag="POH73_000292"
     CDS             22524..23204
                     /locus_tag="POH73_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6886277.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            23229..24782
                     /gene="gntK"
                     /locus_tag="POH73_000293"
     CDS             23229..24782
                     /gene="gntK"
                     /locus_tag="POH73_000293"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDI6886278.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYIMRESKVNQDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDRYIIDYSMASATGMFNLETLDWDVGALELLGISEEMLPELVPTTYVMKGM
                     KERYATLMGLNEDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIEENVAVYQELVSIFINLSRSLTENYEQIADFQR
                     KHMAENKTQ"
     gene            24899..26257
                     /locus_tag="POH73_000294"
     CDS             24899..26257
                     /locus_tag="POH73_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HDI6886279.1"
                     /translation="MFNEIWPLISVVLGIIILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            26639..26741
                     /locus_tag="POH73_000295"
                     /pseudo
     CDS             26639..26741
                     /locus_tag="POH73_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26725..29622
                     /gene="fnbA"
                     /locus_tag="POH73_000296"
     CDS             26725..29622
                     /gene="fnbA"
                     /locus_tag="POH73_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HDI6886280.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKVSETQTTTTNVNTIDETQSYSATATEQPSNATQVTTEEAPKAVQAP
                     QTAQPANVETVKEEVVKEEANPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTALESKPRVTRSTDVAEAKEASDAKVETGTDVTSKVTVEDESKIEAPKGNNVQ
                     PHEGQRVVLKYKLKFQDGLKTGDYFDFTLSNNVNTHGVATTRKVPDIKNGSLVMAKGQ
                     VLDNGRIRYTFTDYIKDKVNVTANLEINLFIDPKTVQSNGQQTITSKLNGKETSGTMQ
                     ITYKDGVKNQYTNVNGSIETFDKEKNKFTHVAYIKPINGNNSDSVTVTGMLTQGSNEN
                     GTQPNVKIYEYVGVENGLPQSVYANTVDSTQLKDVTNQMGDKLKVQNNGSYSLNFDKL
                     DKTYVIHYTGDYLNGTSEVNFRTQLTGYPENRYKTYYYYNNGYTLTWDNGLVLYSNKA
                     NGDGKYGPIVDSNNFEFSEDSGNGSISGQYDAKQIIETEENQDNTPLDIDYHTAIDGE
                     GGYVDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSK
                     HHADVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESN
                     LIELVDELPEEHGQAQGPVEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIK
                     SELGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIIDIDFDSVPQIHGFNKHNEIIEEDT
                     NKDKPNYQFGGHNSVDFEEDTLPKVSGQNEGQQTIEEDTTPPTPPTPEVPSEPGTPTP
                     PTPEVPSEPGKPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVK
                     AVAPTKQKQAKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNHKA"
     gene            29802..30668
                     /gene="galU"
                     /locus_tag="POH73_000297"
     CDS             29802..30668
                     /gene="galU"
                     /locus_tag="POH73_000297"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDI6886281.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIIEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKELGL"
     gene            complement(30795..31103)
                     /locus_tag="POH73_000298"
     CDS             complement(30795..31103)
                     /locus_tag="POH73_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886282.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTTIADKHK
                     TQIAYIFALCISLSAVFLMSAILCFEYDFIALSFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(31247..31513)
                     /locus_tag="POH73_000299"
     CDS             complement(31247..31513)
                     /locus_tag="POH73_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886283.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            complement(31797..33605)
                     /locus_tag="POH73_000300"
     CDS             complement(31797..33605)
                     /locus_tag="POH73_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDI6886284.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIKRANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNNPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     IIVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYSSDGAQLSTDASE
                     LVSRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLKSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGLEGKKKI
                     ESIMTHFRSNPPQEIQGLKVKAIEDYLTSEVYQLDKDTTSQIDSPKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPDFDDVAQKINAMIFS"
     gene            33722..34114
                     /locus_tag="POH73_000301"
     CDS             33722..34114
                     /locus_tag="POH73_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HDI6886285.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEKMSLPLMWEFPGGKVEKNE
                     TEKDALIREIREEMKCDLIVGDKVITTEHEYDFGIVRLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            34116..>36110
                     /locus_tag="POH73_000302"
     CDS             34116..>36110
                     /locus_tag="POH73_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDI6886286.1"
                     /translation="MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKNEFDLLWQNSTQLTQQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPTKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEK
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFEMFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLREGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLIL
                     KAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFL"
BASE COUNT        12704 a         5329 c         6532 g        11545 t
ORIGIN      
        1 attgtataaa tatagaacag cagtaagata tcttctaatt gaaaattatc ttactgctgt
       61 tttttaggga tttatgtccc agcctgttta aatatatatt aatttaaacg tgtttcacat
      121 gtaccagtgt taatgacaga taatgctgcg tttaaaccac cttcaacaag gttttgtact
      181 gcttcatcag agaagaaagc aatatgtggt gttactaaaa ttctttcatg ttcgattaac
      241 tctaataatg ttttatcgtc aatgtctttg ttagtccagt catttgtgaa gtatgctgct
      301 tcattttcat aagtatcaat cgcagcacct aacaaagtac catcgttcac tgcatcgatt
      361 aaatcaggtg tattgatgac tgcaccacgt gctgcgttaa ctaagattgc accttttttc
      421 acatgatcaa acattgtttt atcgaataaa tgatagcttt ctttgttcgc tggaacatgt
      481 aaagaaataa tatcggcatc tttaatagct tctttaacac tatctttata agttaaaaag
      541 tctaaatctt tattaggata agcgtcataa gctgtaattg tagcaccaaa tcctgcatat
      601 attttagctg tagcagcacc gatacgaccc gtaccgataa ttgcaacagt catattttta
      661 actggtttag acatgatttc tgcttgccaa gtaaaatcat gtgcttgtac acggcgttca
      721 atatctggga agcgacgcac taattgtagg gcgatagaaa cagaatactc tgcaattgtt
      781 tcaggtgaat aactaggaac gttagatatc acaatgttgt gttttttagc taaatctaaa
      841 tcatacatat caaatccagc agtacgttgt gcaatttgtt taataccgta agattctaat
      901 ttaggataaa cgtcattttc taacttacca aattgcattg tagttacgcc atcgtaatct
      961 tttaattgat cgactgtagc acttgataat agctctttag aagtagttac ttcgacatta
     1021 ttctttttcc cccaatttaa tgccatctct ttctcataat cacgcgtacc aaagaacata
     1081 atttttgtca ttattaaaaa cctcgctttt aaaagattga aaagtaaatg agtgaaatta
     1141 aagattatgc acattaaaat cacgccacaa tttaattgtg aaaaatatca caaatatatt
     1201 ataacactaa attttccaaa attcaaaagt gtgttttatt gcagaaaact tataacatgt
     1261 gcacaagtta tagtgaattg caaacggatt actttagtct ttttaaaaca tgaagtataa
     1321 tttgtatagc aataaatata aaaattggag gctatgttca atgagcaata tgaatcaaac
     1381 aattatggat gcatttcatt tcagacatgc tactaagcaa ttcgatccac aaaagaaagt
     1441 ttcgaaagaa gattttgaaa caatattaga gtcaggtaga ttgtctccaa gttctcttgg
     1501 gttagaacct tggaagtttg tcgtgattca agatcaagcg ttacgtgatg aattaaaagc
     1561 gcacagttgg ggcgcagcga aacaattaga tacagcgagc cattttgtgc taatttttgc
     1621 gcgtaaaaat gtaacgtcaa gatcaccgta tgtacaacat atgttaagag atattaaaaa
     1681 atatgaggca caaacgattc cagctgttga acaaaaattc gatgcattcc aagcagattt
     1741 ccatatttct gataatgatc aagccttgta tgactggtca agtaaacaaa cgtatatcgc
     1801 attaggcaat atgatgacga cagccgcatt gttaggtatt gattcatgtc cgatggaagg
     1861 ttttagtctg gatacagtga cagacatttt agcaaataaa ggtatcttag atactgagca
     1921 atttggttta tcagtgatgg ttgcatttgg ctacagacaa caagatccac cgaaaaataa
     1981 aacacgccaa gcatatgaag atgttattga atgggttgga ccaaaagaat aaatagaata
     2041 cagtatgtct aaatatataa aattaaaaag ttagcaataa aaaaagcctg cgattacata
     2101 aatgaatcgc aggcttttgc gtgaaaaaat tgtattaata aagtatggat gattattttt
     2161 ctggaacaag gtcagtattt gaatgaactg tgatgtcaaa cccttctggt gccgtaaatg
     2221 tatgtgttga ggcgtcgggt tgataaatat caacatgtgt taatccataa ctttgtgcat
     2281 tgttttgtct tgcttgattg gattgccaag tattagcagc aatatgatgg tgataatgat
     2341 tcgttgacat aaatagcgca cgtggaaaat cagacacatg ttggaatcct aattgttcaa
     2401 tgtaacattg atatgctgcg tctaagtcat gtgtttttaa atgtaagtgt ccaatcatgc
     2461 cttttgctgg cattccttgc caaccttcat cagtacgatg tgttaataag gtttggctat
     2521 caacttctaa agtatccatt ttaactttgc cattttgcca ttcccatgaa gatgaaggtc
     2581 tatcgcgata gacttcaata ccattacctt cggggtcgtt gaaatataaa gcttcactta
     2641 ctaaatgatc accagcgccg atgcccatat ttttttgtgc cacgaaatat aagaagttag
     2701 ctagatcctc agtagttggt aataaaaatg ctatatgaaa aagtcctgct tctcgtgggg
     2761 aagtctgacg gccgtcttct aataaatgta acgttagagt atggccacca gtcccaacag
     2821 ataatacggt tgtgttatcg tcagaacttt taacggatag tcctaaaatg tttttgtaaa
     2881 atgttgtcat taagtctaag tctcttacgt tcagttcaat gtttgtcact tgtgttgctg
     2941 ttttatcgtg aaatgccatt atgaatcgcc tcgttttcta tttttctata agttagtata
     3001 aaaagtatac cagaaaagaa aatgaattga cagcaaaaag tttgaaatgc aaaataacat
     3061 gatgtttcgc aattttatat tgatacgaac aaataaaaaa gcgatggcac tggcgcacca
     3121 tcgcgaggtt tatttaacca agatacacat cttgatatga atcttctttt tctaacatat
     3181 gtttggcaaa tgaacatgag gcaatgattt ttaaatgatt ttcgcgagca tgttcaacaa
     3241 ctgctttaac tagttttttg ccaacacctt gaccaccgag ttcatcagat acgcctgtat
     3301 gatcaatgtt aatttcatta ttatccacaa aacggtatgt gatttcagct aaagcatgat
     3361 tttcatcatc accaatatag aatttgttct cgccttgttt aatttcaagg ttactcatac
     3421 atatcaactc ctatcatgat tgattatagt atttccctat tctattttaa cttaaacgaa
     3481 gtcaaaggtg catgacagtc atgtgacgac attgccacat ctatgtagtc gtttttatta
     3541 agtgcaattt gaaataaaga tgaaaacacg tatcttgaca ttaaattcat tcagctatat
     3601 aatttatctc gaaatcgaaa taaattaaaa agttggtgat gatatggatc gaacgaaaca
     3661 atcgctcaat gtctttgtcg gaatgaatag ggcgttagac acattagagc aaattacaaa
     3721 agaagacgta aagcgatacg gcttaaatat tactgaattt gcagtgctcg agttgcttta
     3781 taataaaggt ccgcaaccaa ttcaacgtat tagagaccgc gtattaatcg caagtagcag
     3841 catttcatat gttgtaagtc aattagagga caaaggttgg attacacgtg aaaaggataa
     3901 agatgataaa cgtgtatata tggcttgttt aactgaaaaa ggtcaaagcc aaatggcaga
     3961 tattttccct aagcatgctg agacattaac aaaagcgttt gatgtgttaa caaaggatga
     4021 attaacaatc ttacaacaag cgtttaagaa actaagtgca caatctacag aagtgtaagg
     4081 cgtgcactaa aattttacat taaaatatct cggtttcgag ataaatacac taaaaatata
     4141 aagagggtat ataaaatgac aaataatcat gaattactag gtattcacca tgtcacagca
     4201 atgacagatg atgcagaacg taattataaa ttttttacag aagtattagg aatgcgttta
     4261 gttaaaaaga cagtcaatca agatgatatt tatacgtatc atacattttt tgcagatgac
     4321 gttggttcgg caggtacaga catgacgttc tttgattttc caaatattac aaaagggcag
     4381 gcaggaacaa attccattac aagaccgtct tttagagtgc ctaacgatga cgcgttaatg
     4441 tattatgaac agcgctttga tgaatttggt gttaaacatg aaggtattca agaattattt
     4501 ggtaaaaaag tgttgccatt tgaagaagtc gatggccaag tgtatcaatt aatttcagat
     4561 gagttaaatg aaggggtggc acctggtgta ccttggaaga atggaccggt tccagtagac
     4621 aaagcgattt atggattagg ccccattgaa attaaagtaa gttattttga cgactttaaa
     4681 aatattttag agactgttta cggtatgaca actattgcgc atgaagataa tatcgcatta
     4741 cttgaagttg gcgaaggagg caatggtggc caggtaatct taataaaaga tgataaaggg
     4801 ccagcagcac gtcaaggtta tggtgaggta catcatgtgt catttcgtgt gaaagatcat
     4861 gatgcaatag aagcgtgggc aacgaaatat aaagaggtag gtattaacaa ctcaggcatc
     4921 gttaatcgtt tttattttga agcattatat gcacgtgtgg ggcatatttt aatagaaatt
     4981 tcaacagatg gaccagggtt tatggaagat gaaccttatg aaacattagg agaagggtta
     5041 tccttaccac catttttaga aaataaaaga gaatatatag aatcggaagt tagacctttt
     5101 aatacgaagc gtcaacatga ttaattggaa tgagagggat ttgtgatgga acatattttt
     5161 agagaaggac aaaatggtgc gccgacgcta atattattgc atggtacagg tggtgatgag
     5221 ttcgatttat taccattagg cgaagcattg aatgaaaatt atcacttgtt aagtattaga
     5281 ggacaagttt cagaaaatgg gatgaaccgt tatttcaaac gtcttggtga aggtgtttat
     5341 gatgaagaag atttggcatt tcggggacaa gaattgttaa cgtttattaa agaagctgct
     5401 gaacgttatg attttgatat tgaaaaggct gtacttgttg gattttcaaa tggatcaaat
     5461 atagcgatta acttaatgtt gcgttcagaa gcaccattta aaaaagcatt gttatatgca
     5521 ccgttatatc caattgaagt aaggtcaaca aagaagttat cagatgtcag tgtgttgctt
     5581 tctatgggga aacatgatcc aatagtgcca ttagctgcaa gtgaacaagt cattaacttg
     5641 tttaagacac gtggggcaca agtcgaagaa gtttgggtga atggtcatga aattacagaa
     5701 actggattaa cggctggtca acaaatactt gagaaataac agttctatta agaagcggac
     5761 agatggaaaa gatttttact tttcatctgt ccgctttttt gattttgaag tgttgtacta
     5821 aattttacaa tagtatagat attttaatcg atatgagatt tgccggtaat acgcttaatt
     5881 aaacctttat agagtacagg tatgagtaag atgaaaccga acaatcccat aatagggaat
     5941 acttttccaa ttaatgaaat gaaaccgata aatgtactaa tataagtgat gacagccatt
     6001 gtaataataa tgatgaagta acgtctgcta aatggaacgc tgaaacgtga cgcaaatgca
     6061 tacattaatc caacaacagt attgtagatg acaagtatca taatgacaga cataataata
     6121 ccgattgacg gagacatttg tgtcgctaat tttaatgtag gtaaatcgac gtgtttaatt
     6181 ttatcgaatt gagaaattaa acctaggtta atcatcatga gtaaaaatgt aataattaaa
     6241 ccgccaatca agcccccgta taacgttgag tcacgatact taactttact acccatcact
     6301 gataagaagc tgaaggcagc agcaatttgc aagcttgcat agttaatcgc atcaaaccac
     6361 catccaggtg ataatgattt ctgcttatga atctgagcat cattattagc ggcagtaaaa
     6421 tcaaaatgac tcgttgtgaa atagtagacc gcaatcataa tgacaatcgc aattaaaaat
     6481 ggtgtaacac cgccaagcac agcaattaaa cgatcgaatt ttagaaacag tgttgctaaa
     6541 ataaacacga ctaatatgag tgcgctcagc caatacggta agttgaaact ttgatgaatg
     6601 gttgacgcgc cacctgcagt cataataata gctaaagaca acataaacat tgtcaaaata
     6661 atatcaaaac ctcttgcaat agagggatat aagaaatagt taattgaatc agaatgattt
     6721 ctagacttta gatgatgacc tgtatgcatg acaatcattc cacctaaagt aatcaatagt
     6781 cctgtcacaa taatgcctga aatgctatac gcgccatgac ttgtgaaaaa ctggaaaatt
     6841 tcttgaccag tagcaaagcc ggcaccaacg acaacaccaa caaaggcaaa tgccacaata
     6901 atggactctt ttaagatgcg catgatttaa aaatgtccct tcgtaatttt aagtaatata
     6961 gaaaatgtaa catacatgtg aatgaaaaat atagtactaa tatagtattt tgttaaattg
     7021 gagtagaagc aagggcgtcg atcattttat taatttatta gttaattttg cattttttac
     7081 tgtaatgttg taataataca gttaacaagg aactagcata gatacaccaa tcccctcact
     7141 actcgtaata gtgaggggat tttttcggtg tagctaggtc gcctatttac catcgcgttt
     7201 gcgtagccaa tgcgtaaaca cagcacctct aaataagtgt acgacacgtg catcaaaagt
     7261 cgtctttagt ctaagtaacg atcatgcatt aacattttca aaatatctat ttgagcctga
     7321 agctctttac ctttatttgt attgcggatt tttttacgtt gtaattcttt atcaacaatc
     7381 cgtttaattg acagttcgtc gataccttcg gaaagaattt tttcttttgc gttaaattgt
     7441 tgatgtgcaa cgagttgcat tccgaatgaa ttatacaaca atgtatagcc tgcaatgcca
     7501 gttgttgact gataagcttt tgaaaagccg ccatcaatga caagcatctt tccatcagct
     7561 ttgataggat cttcgccatt aatttctttc actggtgtgt gaccattaat aatgcgtcct
     7621 tcatctggat ttaatccgaa atcactgagc attttacgaa ccatattcac atcttcacga
     7681 agatgatagt acggattctt ttcttcttta tgagaagctt tatctgcaat aaagtatcgt
     7741 tcaaacgtag tcatcgcacg tttaccaaat agtgaagagt atttcccagt ccataaatac
     7801 caaactaaat cagtggataa gtcatcagta ttttcttttt catcaaatga tttacggaca
     7861 tgatattcaa acacatctaa taactcttgg ccactgtatg tctgaccatc aatttcgaat
     7921 gattccatct caccattttc atcaactgga atacaaccat gaatgagtaa attgccatta
     7981 caaggtaagt aaagtgagcc tttacgcatc aagaaagaca tatgacgacg taatttttca
     8041 gattgctgga atgacaataa tagtttgttc atgacttctt cttcttcagg tagtaacttt
     8101 gctgggttat cacggttaac agtttggaag catgtatctt ttaatggata tgtttttccg
     8161 tatactgtta tttcattagt atcataatta actttttcaa gtacaagtcg ttcgtccatt
     8221 tcaaagtttg gacgtcgctt aataaccggt atttccagtt tgaattgaat catcgcaatt
     8281 gcttgatgaa ttttagtaat ctgactttct tcgcgttgag ttaaacgttc atgtttgtca
     8341 ggcctttttt taggtttaaa agcaggatta tctgcatcat agtatttttc ggctaatgtt
     8401 agcaaaggtc ttaaattaat accgtatgca tcttcaacga tatctagatt gtcataacgt
     8461 gcacaaatac gtagtaaatt agctaaacat actttagacc ctgcatacgc accaacccac
     8521 agcacatcat gattacccca ttgaatatcc agagaatgat aattaatcag cgtgtccatg
     8581 attttatctg gttgcgggcc acgatcataa atatcgccga caacgtgtaa atgatcgacg
     8641 actaagcgtt gtacagaata cgcaagccct ataattaaat catccgcttg cttaagctca
     8701 atcacttggt ttacaagtgt ttcgtagtaa gattttttat ttttaaattc gttgttctta
     8761 tataataact cttctacgat gaaaacgtat tgctccggca aggcacgtcg aagttttgaa
     8821 cgcgtatatt ttgaagaaca atatttaatt agctgaatga ggtgttcaat cgtcgtaata
     8881 taccaaacat ttaattgtcc ataattttga aaatcacatt taataagttg caatttgtct
     8941 tctggatagt aaacaagggc agtcaaatcg ttaagttctt tcgttgataa cttgtctttg
     9001 aaaatatcat taattttcgc tcgcacgttc ccagaaccgt tgcgtaatac gtgttggaaa
     9061 gcttcatatt caccatgtaa atcgctgacg aaatgctccg tacctttagg caattctaaa
     9121 atagattcta aattaataat ttcagtcgca agcttttctg gagtatcgaa atgttgagat
     9181 aataaatcca aatatttctt ttttaattct ctttcagtaa tttgagtcat tgtgtgtcac
     9241 tcctgtagta tatttcaatc attggtttgt aaaattaata cgcttacaat gattatcaag
     9301 aaaacgttaa catttttcaa taagtatgct tgaattgata aaaatagagt aaacatattg
     9361 tgaattttag tgttgagaat ctgtggttgt atcattcata ttagtagcgt tgaagtgtac
     9421 ccgcataaat atgaagtgat aaagttgttg aagcggcgtt gaaatatcat gatagagata
     9481 taagcatccc atccgggaga ataattttta tattaaagca gagttgaaat agcttggcag
     9541 agatacgatt atcgtttaag tgagcatatt ttgtacatta caagtggggg gattttgaaa
     9601 tatattaaag atagaatgag tggcatgctg acatatcaaa aatccctgca ccattatttg
     9661 ggtgaaggga tttgaaaata attttgaatt acgatgaaat atgtgtgtat ttattcatct
     9721 aatactttaa gttactaaat atttttcttc ttgaaaataa atactacaag tattaagaaa
     9781 atagaaatat aagcaatatt accaataaac aattcatgaa gttctaattt cgtcgactta
     9841 ctaaagcctt tttcaactgt ttgaagcata atattcatca tattaatagg attccacttt
     9901 agccagtcta ttttttctaa aagtgccgtt tgaataactg ctaaaataga acttgcaaaa
     9961 taaaaaacaa tacctacagc aatggctaca cccgttgaat tagttgcaga tgataatagc
    10021 aacgttaagc ttaaaactaa ccaaacacca acaaatgtac ctaaactaac taataataat
    10081 tgattcaata aagataattg attaccgcta ctttcaaata tatttaaatc attaaagaat
    10141 aaagacccaa taacaattga agcaataatt gtaataacaa aataaattaa agaaataata
    10201 aataatgtga tgattttgct aacaatcata gttgttcttg aatattcacg atagagtaaa
    10261 tttttaatcg taccgtaatg aaattccatt gaaatgattg tacttgcttg aataattaat
    10321 aaaaatgcaa accatgaaaa accattataa gcagatgtaa aagattcctg tggtgtaaaa
    10381 atttcattgt attttgtagc aatataacct tgagcaacca ttagtagaat aaagacaata
    10441 ggtgcgataa aagttgattt ctttttaaat aatttgaaac attcttgttt aattaaagtt
    10501 cccattttat gcttgatcct ccctttctat gatgtctagt aatacatttt ctaaatcatt
    10561 atctttaaat tgtttaagtt cttcttccga tgtttctgta acaattttac ctttgttaat
    10621 aataaggata gagtttgtga ttttaactaa ttcacttaaa atatgactcg aaattaagaa
    10681 agtgacacct tcttgcgctt tttggacaat caattcacgt acatctcgca cagcttttgg
    10741 atctaagcca ttcattggtt catctaagat aataaattga ggttcattta aaaatgctat
    10801 agcaattcct aatttttgtt tcataccaag agaatacgtt ttagcttttt tatgaatgta
    10861 ttcatccata tgaagttgtg agacaatttt atcgatatct tgagtgtttt ttgattcatt
    10921 caataacttc aagttttcat atccagacat aaaaggatat attcctggat tttcaatcaa
    10981 tgcaccgata ttgcttttgc tatccttgct gttaataaca ttaaaattcc cgctttggaa
    11041 actagagtaa cctaatataa ctttcattaa agttgtttta cctgcaccat tcgctccaac
    11101 aagaccaact atttgtccgc gtttcagctt aaatgataca tcgtcgagaa tcgttttatt
    11161 acctatcttc tttgttaaat gttctattgt taaaacatcc atatcactat ctcctttgct
    11221 attaaataat ctgaatctta agattatata tataaaatta attttatcac atagcaagtg
    11281 ttataaaaat atggatttaa aaaacttaaa taatataaaa tataaatatt aatatgagta
    11341 tttttgcagc atgcaaatgg cattgctaaa tttattaaaa tgcgcaattt gttatgagtg
    11401 acttttttga atagaagaca tattaaagtg acgaaaacag tttgtgtgga agacagggta
    11461 aattctgctt atataaaatg atagtttgta ttatagatgg atttgtgaga tgggtatacg
    11521 gaattaaata agtaataagt aagataggtt gtgtgactga atattttctt aagttttagg
    11581 ttgtcaaatt aagtgcaaca attcgccaca taaaaattaa aaaagggaat ttccaatcaa
    11641 aacatttttt ggggaattcc ctttttaata gtactcatta ttatctttta ataaaacttc
    11701 tatattcata caatgaaata atatttgatg atatagatca tattgtcaat atttgtaaat
    11761 gtgtataata tcccaatatc ttataacaaa atgcatttta ggttttttat gaaatgtaat
    11821 gcgtattgtg cttaatatta taacacgcat gtataaaatg aattatataa agggtgataa
    11881 atgttaacta agttcgtatt ctatttataa tttcacttaa tttttttaag ctttggtcaa
    11941 tttcttcctt gtcgatatat gagtatgaaa agcggatata gcttttagag ttaatgtcat
    12001 atatttctcc tgggtgtatt agtatatttt cttttattgc ttcgttaaat aagagcttta
    12061 tatttactgg aacagatagc ttaaaccaga tataaaatga gccttggggt tcattccaat
    12121 atccaaactt agatagatgt ttctttaatg actttataaa aatagatttt cgtaatttta
    12181 gttcgatttt taatttatat aaatgatctt catgataatt acttaaccac gttgtggcaa
    12241 tatactgtga tatagaactt gcaccataat cattttgcat ttttaaatcg gctaaatgat
    12301 gaattacttt ttcatctgct ataatccaac caatacgtaa accgggactt acagttttag
    12361 ataaactacc taaatataaa acattatcac tattttttaa agctttcata gaaatttgtt
    12421 ggttagggct gaaccataaa tcagagtata tactatcttc cacaattgga ataccttctt
    12481 tatgacattg atctataatt ttttgcttct cttctttact ataagtattt tgagtagggt
    12541 tatgcagcgt aggtatacag taaaaaatac tgtgcctata atcactattt aatttaaaga
    12601 tattgttaat attctgtttg atgtaattga tggacaaagg aataattttc gctctgatat
    12661 tatgccaagt acgtatggaa ttaatgtatg atggtgtttc aattataatt ttagattgtg
    12721 gaattaacag tccgtctgca attagtttta aaccttgtac agcaccggat gtaatacata
    12781 tattattgat attacaatca actcctcttc gcttcatata ctttactatt tctatcctta
    12841 actttagatt tcctttaggt tcttcatagt tagtttgtaa agattcttca atatcattag
    12901 ttaatatttt tttaaatata tggtttggaa ttagttttgg tgatagttct ccagtaccta
    12961 atcggacgat atcagtatta aattctattt cattaatttt ttgaatataa tactggttat
    13021 ttttagaaaa gctgttgcct atataatctt gccaatgaat attattggta atataagaat
    13081 tccatttata ctgggataca taaatacctt ttttttcaat tgtatctaag atgccatagc
    13141 tttttagaat atctaaagct tgtataactg tggatttatt tacattatag tacttagcta
    13201 attttctttg cgagtacaat ttatctccag gagataaaat accgttgtta attttattaa
    13261 taatatcatc aataattttt ttatattgaa atttagtcat aaatgtcatc ctcaatctgg
    13321 ttggaatttt tattagaatc tggtaggttt tttaattttc agattattat aacatattaa
    13381 ataagggagg cggttaaatt gacactaaaa ttagaaaaag ctgtattttc aatggagata
    13441 aaaaaagatt tgaagttatt aatgaaaaag gataattacc acaatatttt cgctttgatt
    13501 tttgactggt tggtaattct cgggagtgca tgtataagta tctatatgag taatctcatt
    13561 atttatatta taagtattat tttaattgga agtcgaatga gggcgtttga taacttaatg
    13621 cacgaagcat gtcatcgttc attatttaca aataagtttt ataataaatg gattgcttgt
    13681 cttttagtag cattccctat atttacaagc tttactacat attgtaattc tcattttaaa
    13741 catcatagaa atttatggga tgaggaaaaa gacccagata ctaaacgata tcgcattgta
    13801 ggtttagata agccgcaaaa agatgtgaaa aaatttataa ttaatcacat tgtaaaggta
    13861 ctatttttat tccatgtgcc taaatatgtt ttaggtacag tttcagctaa cttatacagt
    13921 agggatacac ctaaatcaga aatatggact agaaatattt tctgggtaac aattattacg
    13981 ttatcaatta tgtttaattt ttggttatat gttattcttt attggtttat accactttta
    14041 acaacttttc agattattcg ttattgggca gaaatggctg aacactctgg attgaataat
    14101 gatagtgagt taacagcaag tcggaatact tttggtatgc cttggacaat atttttattt
    14161 catcctcacc atgataattt tcacttagtt caccatttat tcccagcaat tccacattat
    14221 aatttgaaaa aagcacatat tatattaatg aaagatcaaa aatatagaga agcacaccat
    14281 tgtactgggt tcttcaagac gacactaccg ggtttttata gtgtagtaaa agatatttgt
    14341 acaccgaaca ttgttgggaa gggggaggat gcgaattgat acaaccagtt ttacacggta
    14401 ttttattagc attggggctt attttgcccc taggagcgca aaacgtgttt gtatttaatc
    14461 aaggtgctaa tcaaaaaaaa atgataaacg cattgccggt tgttataacg gctggattat
    14521 gtgacacttt tttaataata attgctgtac tgggtgtttc actaatatta atgtcattac
    14581 cagttctaca attatttatt tatatagttg gtttgttttt tctattgtac atggcttggt
    14641 ctttatggaa agaaaagcct aacactctag agaactatga accgatgagt acaaaaaagc
    14701 aaataggttt tgctatatct gtttcgttgt taaatccaca tgcaattatg gatacagttg
    14761 gtgtaattgg atcaagcgca tctgtctatt ctggtttaga aaagatactt ttttgtattg
    14821 ccacaattat ggtttcttgg ctatggtttt ttctgttagc aatattaggt aaggttttag
    14881 gttctataga tagaacaggt aaatatatat tatttttaaa taaaatctct agtataataa
    14941 taattgctgt tagtattgtt attgtaaaaa atatattaaa gttaatttta taatataaaa
    15001 gtgcatatta gttataattg gaaaaacagt aagtgatatt taaaaatgaa aagattttaa
    15061 tattataatt aatcttgagg tgttgagctc cttagaatta gttgacaaaa aagattggaa
    15121 atcttaactg aaaaaatgta acattattat gaaattatat aaaaatataa agaagaacct
    15181 cggaggaata aattcgaggt tcttctttat gctaacaaca atattatttt actctcttac
    15241 gtttgccaat gtaacgtata gcaaagtaaa tgactgcgat aatgatgaca acatacataa
    15301 tacgtgaata tgtatgaaga ccagtcatca acataccaaa gctatcgctc aacgtgcgtc
    15361 ctagtaaaat taaaccgaag ttccaaattg ttgtacctat taatgaaata acggtaaatg
    15421 tcacaacatt catgcggttt acaccggctg gaatggtaat caatacacgt aatacaggta
    15481 taaaacgaca gataaataca gcccatacgc catacttttt aaaccaatca tttgctcgct
    15541 ttaaatcctt acttttcaat ttaatccact taccgtgtcg atcaataaag cgatatagac
    15601 gttcttctga aatcaaacga cagatataat ataaaattaa cagtcctata aacgatgcga
    15661 tggttgcaat aataaataag gttaaaattg ataaatgtga tttaacagac ataagaccag
    15721 caaatgttag aataatttcc gatggtacga taggtaaaac attttctaat aaaattaata
    15781 taaaaatggc tgcataccca aaacgactaa taaattcagt gataatttgt tccattatta
    15841 aaaataagct cctttaattt tcagcaattg cagacaaata gaaactggat aaattcaaat
    15901 gaataatgaa tgatgtcgta tgcgatgttg aaacaagcat ttgtcttaaa atgtattata
    15961 agcaataaat caaaaaattg cttgctataa gtacgaaaag tcaacaaatg atcatctaaa
    16021 taacaggcgc tgcacaatga atgagtaatg tagttttatg caatttaaaa tgaatgtcaa
    16081 atatgtaaga aatcctgcaa tatacgtttt aaaacactag tattgcagta tttttaatta
    16141 acaaatgata ggctactatg taactaaaag ttcctttata tcgttttaat agcaaaagtt
    16201 aagcggaatt taataaaatt tttatagatt ctattgtaaa cgctttccat gtctgataat
    16261 attataaaaa ataactttgg tacaattttg tgacaaagta ggggtggaca tgccagatgt
    16321 ccgaaaattt taacgtttca gggtaagttt taaaaaatta attaattagg gaagtaggaa
    16381 tagttatgaa ggaaaaacga actaacgtaa ggtggatgtt cgcgcttgcg ttcttcttta
    16441 ttggggttat tgcgtatatg gatagagcaa acatttcata tatcgctaag caaatgatgg
    16501 atgatttagg gatgacaaaa ccacaatttg gtttattggc atcattcttc tctctaggtt
    16561 atgcattaat gcaagtacca tcggggatgt tggctgaaaa attcggtcca cgtaagatga
    16621 ttacaattgc attagtttgg tggagtgcat ttacaatctt aacgggtatg attaagaacc
    16681 acggtttaat ttatttagtg agattcttat ttggtgtagg tgaggcgcca atgtaccctt
    16741 ctaatgctgt gtttaactca ttttggttct ctaaaaatga aaaaggtaga gcatcaagtg
    16801 cattattagc aggatcatat ttcggacctg tattagcacc aatagttaca attgctattg
    16861 ttaacgcatt taactggcaa gcagtatttt atatttttgg tgcagtaggg attttaatgg
    16921 ctgtattatg ggcgattatt gctaaagact tacctgagca acatagaatg gttaatgaag
    16981 cggagaaacg tttcattatg gaaaaccgtg atatcgtagc tactgaaaag tcatcaccac
    17041 catggaatga tttctttaaa cgttttagct tctatgcaat tgcaattcaa tactttgttg
    17101 tacaatttat cattacatta ttcttaattt ggttaccgac gtatttaaca gaagtattcc
    17161 acgttaactt taaagaaatg agcattagtt cattaccttg gttattaatg ttcttcttaa
    17221 tcttatcagc gggtgcaatt tctgaccgtg tattaggatt aggtcgttca aaattcgtag
    17281 ctagaggtgt aattgcaatc gcaggattta ttgtatttgc agtttcaatt atctttgctg
    17341 tacgtacagg aaatttatat gtaagtattt tctggttatc actaggtctt ggtggtatcg
    17401 gtatttcaat gggtatgagt tgggcagcag caactgattt aggacgtaac ttctctggta
    17461 cagtatcagg atggatgaac ttatggggta atataggtgc attaatcagt ccgctattag
    17521 caggtctatt cgtagaacat ttaggttgga caatgacatt ccaattgtta atcgttccag
    17581 cagtaatcgc tgtgattatg tggttctatg tgaaaccaga tcaaccttta attgttagtg
    17641 atgataaagc aatagaaaaa taatttaatc atgcaataag ctttcacata gttggggctt
    17701 attgcttttt tcgttgaaat tgaaaaattt taaaacagat atggtttaag atgaacggga
    17761 agttattaaa ataatatatg tagaagaaat aaagttttaa aaccttagtc aggtaactat
    17821 tgtaaaagta caaataaatt ttaactagcg caaaggtggg cgaccaaagg ttcaacgatg
    17881 ttaaataaca ttagaaaata attttaattg gactttaaaa gtttttaaat ttagataatt
    17941 gagcataagg tgttataatg acatatgttg cgtaattgaa atttatagca acatactcaa
    18001 attaactatg ctaataaaat gattatggaa atattttgac gaggaaggga gaagtcgaaa
    18061 tgacatcttt ttgacatcac ttataaaaat taatcgactt aacttagact tttataaagg
    18121 gataagacaa ggactgttaa tgattattcc tgcaatcatc ggttacttat gtggtaattt
    18181 ccaatttgga ttattagttg caaccggaac tctagcacat atctacgttt ttaaaggtcc
    18241 gtcgcgatct aagctgcgaa ctgtaataat ttgtaattta gcgtttgcaa tatgcatgat
    18301 acttggtacg ctaacagcca caacgccgct cgtttttgga atgacattat taattgttac
    18361 ggttatacca ttttatatat ttaccgcctt gaaaattgct ggaccgtcat cgacattctt
    18421 cattgtgaca ttcagtttac ccattaactt acctatagca cctgaagaag cattatatag
    18481 aggctttgcg attttagtag gtggtgtact tgccactatg atggtactca tcacaatctt
    18541 attgtctaaa aacaaagctg aagaacaagc cattcaaaac gattttaaac tcatatctaa
    18601 gttattgcat gcttacaatg ataaatctgc attttcacaa gtagcaaaaa cagcggtgga
    18661 tagttttaaa gcgtcggata aactactcat cacttctaat tcaagtagtg ataaattaag
    18721 cggacgtttc caaaaattat tgttattaca cacatcagca caagggatat attctgaatt
    18781 attagagctg aatgctaaac aaatcagacc attgccagat gagttaattg aaatgatgga
    18841 tcatatcatt acacagcgag atgatcgcaa taaaaatgtg agatattggc gcaaagaagt
    18901 aacagtaaca gaggaatttc aaaacttatt caaccatatc ttgaaaattg acgaaatggt
    18961 gcatgcaaat gaagcacgta tcgcatatga agctgaaatg cgaaaacctt tatatagtaa
    19021 gcgtatttat caaaatttaa cattagattc aatcgtgttt agaaatacat tgaggtatgc
    19081 agcgattatg atgatagcga tatttatcgc gttaatgttc gattttgaaa aagcatattg
    19141 gataccttta tccgcacata cagtattatt aggaacatca actatacatg caattgagag
    19201 aggtatggca cgtggattag gcaccatttt aggtgtgcta gtactttcag ttatattgtt
    19261 gttttcaata ccaacacctg ttgcagtcat tttaatgggc attgcagcat tatttactga
    19321 agcgttggtg ggagcaaatt atacaattgc agtagttttt attacaatac aagttatttt
    19381 aatgaacgga ttagcatcac agaacttaac aattaacatt gcgtttccaa gagttattga
    19441 cgttgcaatg ggtattgtga ttgcaatcat aggtttattt gtccttgggc aacgtaccgc
    19501 atccgcattg cttccttatg taatggctga agttgttcgt aaagaagcaa cgctcttcca
    19561 ttatttattt tctgaaaacc aatataaaga tgatgtgtat cagaaaaaca cggctatgaa
    19621 cctatctgtg aaattaaata atatgacgca agtttataat gcagctaatg gtgaattatt
    19681 tagtgataaa acattgattc aaaattatta tcctagctta ttcgcattag aagaaattag
    19741 ctttatgtta aatagagcca tggctaatga agatagactt acaataaatg aacaattaat
    19801 gggcgagtat ttagcaacat ttgaaaacat cgctaagcat ttagaattaa atacagaatt
    19861 agaaataaag atattaccag atttaccaca gtataattat attcaatcag caatgatgaa
    19921 tattcaacac aatggttttc gtgagagaga caagaacgtt taaaataaat tatttatcag
    19981 gtcagactaa cgtttgtctg aagttattat gacgggagtc taaaaaattg tgtttaatat
    20041 gtttcatttt ataattatgg tggcatttaa catgaattat aacatattgg aggttaagga
    20101 aatgcccttt acaatgaaag gttaccccga aaaatggaaa gaatttgaag taattaaacg
    20161 gcaaaaggcg atagaaattg cgaatgcaat gttaaaagaa ggatatcttg aaaaagatgt
    20221 tgttcctatt gcggcgaaaa aagcgaaaga ttggtatcga gcgttatcga aagaagaaat
    20281 caaagcatta gaagaagaga acttcgtaca attccataca gcaacaatgg aaaaaccaga
    20341 ggtggatagt gaatatgcaa caactgataa tgaagaacat attcaagaac aatcaaatca
    20401 agataaatcg tactttgata gcgaatcaat tggcaaccaa agtggtggcg ttcgacaaag
    20461 agaaccgctt atggatggtc cgcatccaaa agttgtggaa tcatttaaag acgtataaca
    20521 tctctatcaa ttaagcactt gtacctatat aacggtaaca ggtgtttttt tgcgttaaat
    20581 cattttaaaa ttacttccta taattgaagg ttttgataca taattcgaat tgtttgagtt
    20641 tctttgtgct gtttcagtat cagcccaaag ttcgatagtg tattagtatt atcttaataa
    20701 aatgttaggt acaataaaga tgattatata tcggaggtaa gtataaaaat gtatgtagat
    20761 cgaaaaccat cactatattt agaggatttg cgacatgatt ttaaaaatag tttaagtaaa
    20821 tttgaaaatg gtgatgaagc atttgatacg ttattaggtt tcgtagagtt agatcatatt
    20881 tattcatcag cactaaagga aataagcaca aaactgagta ttttagatga caatttcaat
    20941 cacatttata aacacaatcc tatacatcat atggagcgac gtgtgaaaga aatgcgtagt
    21001 ttaatagaaa agcttaatcg taaaggatta cagattagcg cagaaactgc caaagaacat
    21061 atactggata ttgcgggaat tcgcgtagta tgtaattact tagatgatat ttatttgatt
    21121 gaagagatgt tgcttaaaca agaagacgta caattgataa aacgtaaaga ttatattcag
    21181 caccctaaag aaaatggtta ccgcagttta catatcgttg tatccattcc agtcttttta
    21241 gcagaacgcg ttgaggtatt gcctgttgaa attcaaatta gaacaatagg tatggatatg
    21301 tgggcaagtt tagaacataa aatacgttat aaaaacaagg cagagacgga aaagtatcga
    21361 gatttattga aagaatgtgc gacagagatt actgaagttg aaaataaatt acaacaaatt
    21421 cattctgaaa taacagagta gacgtttagt aggtgtgttg cttaatttga gtgattttgt
    21481 aaatcaacat ggtatgaaat cacaaaaata agacgcctag tataataaat ttcttaaaaa
    21541 tgtggaaatt atacttgctc gtgacgtcgg gtcatcaact atggtgattg taggaggtgg
    21601 aatctatgtc taactattcg actggagaac tcgcaaaatt atgcaatgtg acaacacgaa
    21661 cgattcaata ttatgatcgc aaaggtattt tgaaaccaca aggatttaca gaaggaaagc
    21721 gtcgtgtgta tacagaacaa cagcgacaaa cattagagtt aatcttatta cttaaagatt
    21781 taggttgtgc gttaagcgat atagatatgt tgctaaaagg tgaaggtact ttgaagacac
    21841 tcaatacctt actaactatg aaacaacaag aaattaatca acaagtcaaa cagcaacaag
    21901 cggtattaga caaaattaaa aatgttcaat attacgtaaa tgaagcgtcg acgtctccaa
    21961 tcacacattt aaaagacata gagcatgtca tgagtaaatc agctgaaatg aaaagtattc
    22021 gtcgtaacat ttggattagt gctggtatta taggaataat tcaatattct agcattttga
    22081 gttcaatctt gatgaaaaac aaatggccgt tcttaattgc tttaccattt atgatcggtt
    22141 atggcattgg tattactatc tactatcaac gcaaagttgc ctatttatgc cctaactgcc
    22201 agcatatatt ctcaccgtct ttgtgggcag ttatcaaagc gaaacatact gcgacaacac
    22261 gtcgattcga atgtccaaac tgtcatgaaa cgcattattg cattgaagta cctaaaacac
    22321 atatgggtac agaataatta gacacatccc acatacaaca taacaattaa atcattcgtg
    22381 tgatagtgac cattcccttt aaatttacat tgaagagggg tggtcactat tttattacgc
    22441 gtaaagaaag cgcttgcaaa cacttaagaa ctatattaac atacttgtat acaagtatgt
    22501 cgtgtaatga aggagtggct ttgatgatgt atggatatcc agagaaatgg ttggaaggta
    22561 tgacaactgg agaaggtatc gcagcagaat tacgcttagg cattgtgaat ggtcacatag
    22621 ctgaaggtac gttactcact gaaaatcaaa tggcaaagca atttaatgtg agtcgttcgc
    22681 caattcgaga tgcatttaaa ttattgcaac aaaatcaact catccaatta gaaagaatgg
    22741 gtgcacatgt gttgccgttt ggggaacaag aaaagaaaga aatgtacgat ttgcgactga
    22801 tgttagagtc atttgcattt tcaagagtta aaaatcaaga gcgactacct atcgtaaaag
    22861 aaatgaagaa acaacttgaa atgatgaaag tggcagtaaa atttgaggat gcagaatcat
    22921 ttacgaagca tgactttgaa tttcatgaaa cattaatcaa agcatctaat catcaatatt
    22981 taaactcatt ttggagtcat ttaaaaccag taatgatggc gctcgtttta acatccatgc
    23041 gacaacgtat gcaacaaaac ccgcaagatt ttgaacgcat acatcataat catcaagtgt
    23101 ttattgatgc agtggaacaa tacgacagtc aaattttgaa agaagcgttt catttaaatt
    23161 tcgacgatgt tggtaaagat attgaaggat tttggttgaa ttaaaatgaa aagaaagaag
    23221 gatttgtcat gaaatatatg attggtgtcg acattggaac gacgagtacc aagtcagtct
    23281 tatatgatga aaatggaact ttcatcatga aacatcaaat cggctatgat ttacacacac
    23341 caaacgttga tgtctcagaa gaaaacccag atgaattatt tgatgcggta ttaatgacaa
    23401 ttaaatacat aatgagggaa tcgaaagtta atcaagatga tattaaattt gtgtcattta
    23461 gtgcgcaaat gcatagcttg attgcgatgg atcagcaaca tcaaagatta acaaataata
    23521 ttacttgggc agataaccgc gctgcaaaat atgcaacagt aataaatgaa gtgcatgatg
    23581 gcaatgcgat ttatcagcga acaggtacgc ctattcatcc tatgtcgcca ttagcgaaga
    23641 ttttttggat gaaacatgaa tggcaagatg tatttcaacg aactgctaag tttgcagata
    23701 ttaaaacata cattttctat catttatttg atagatatat cattgattat tcaatggctt
    23761 ccgcaacagg gatgtttaat ttagaaacat tagattggga tgttggggca ttagaattgc
    23821 ttggtatttc cgaggaaatg ttgccggaat tagtgccaac aacgtatgtg atgaaaggta
    23881 tgaaagaacg ttatgcaaca ttaatggggc ttaatgaaga tacaccgttt gttattggtg
    23941 cgagtgatgg ggtcctttct aatttgggtg tcaatagtgt tggtaaagga gaagttgctg
    24001 tcacaatcgg tacatctggg gcgattcgta ctgtgataga taaaccacgt actgattaca
    24061 aaggtagaat attttgttat gtcttaacag aggaccacta cgtcattgga ggccctgtaa
    24121 ataatggtgg tgtcgtattg agatggttgc gcgatgagtt gttagcaagc gaagtcgaaa
    24181 ctgcgaaacg tctcggtgtt gatccttatg atgttttaac tcaaattgca aagcgtgtta
    24241 aaccaggtgc agatggttta atattccatc cctatttagc tggagaacgt gcaccgcttt
    24301 ggaacgcaaa tgcaagaggt tcattcttcg gtttaacttt atctcataaa aaagaacata
    24361 tgattcgcgc tgcattagaa ggtgttcttt acaatttgta taccgtctat cttgcgctta
    24421 ttgaagtaat gaatgaaaca cctaagatga taaaagcaac aggtggtttt gcgaaaagtg
    24481 aagtatggcg tcaaatgatg tcagatatat ttgacacaga gttagtggtt cctgaaagtt
    24541 atgaaagttc atgcttaggt gcctgcgtac tcgggcttaa agctgtaggt gacattgaag
    24601 atttttcaat cgtttcatcg atggtcggtg ccacaaacaa tcatacgccg attgaagaaa
    24661 atgtcgctgt ttatcaagag ctcgtatcca tttttatcaa tttaagtcgt tctttaacag
    24721 agaattatga acaaattgca gattttcaac gcaaacatat ggctgaaaat aaaacacaat
    24781 aaatacgtca ctcaagcatc ttagataaag ttgttgtgcc atgactacta tatattatgt
    24841 ggaatacgtg cgcgacgacg gtgttacatt ctattgaaaa gagaaagagg ggaatgtcat
    24901 gtttaacgaa atatggccgt taatcagtgt tgttttaggt ataattattt tattagtatt
    24961 aatcattggg tttaaattaa atacatttat ttcattaatt atcacatcga tgattacagc
    25021 gttaatgcta ggtataccat tgactaaaat tatggagacg attgagaaag ggatgggcag
    25081 tacgctcggc cacattgcac tgatattcgg tttaggtgcc atacttggga aattacttgc
    25141 tgatggtggt ggtgcaactc gaattgcaga cacgttgatt caaaaatttg gtcaaaaaca
    25201 tgtacaatgg gcaatgcttg ttgcagcatt tatcgtcggg attgcattgt tctttgaagt
    25261 aggtttagtc ttactgattc cattagtatt tacagtagca aaacgcgcaa atgtttcagt
    25321 gttaaaacta ggattaccta tggtaacagc tttatcagtg acacatggct ttttaccacc
    25381 acatccagga ccggtagtca tcgcaaaaga attaaaggcg aatgtaggag atgtattact
    25441 atacggtatg attattgcca ttccagttac actcattgca ggacctatat ttaacaaagt
    25501 tgcacaaaaa atgattccgt ctgcgtatac acgagaaggc gatatttcag cgttaggtgc
    25561 acaaaaagaa tttacggatc aagagatgcc aggatttggt atgagtttat taacagcaac
    25621 attaccagtc atattaatgt tagtgtctac gataacgcaa cttgtaaccg ggcacgacaa
    25681 acctacaaat ctatttgaat ctatcattta tatgatcgga acagcaggga cagccatgct
    25741 aatagcagta ttatttgcaa tcgttacgat gggattaatg agaaaacgta agatgaatca
    25801 tattatggaa tcagtgacga atgcgattta tccaatcgga atgatgttat tgattattgg
    25861 cggtggcggt acatttaaac aagtattaat tgacggtggt gtcggaaata caattgctaa
    25921 aatgtttgaa ggtacagaga tgtctccgat tttactagct tggattgttg cagctgtgct
    25981 acgtatcgca ttaggttcgg ctacagtagc tgcgatttca actacaggta ttgtcttacc
    26041 attattacaa tcatcagatg taaatgttgc attagttgta ctagcgattg gtgcaggtag
    26101 tgtgattttg tctcatgtta atgacgcagg attctggatg tttaaagaat attttggttt
    26161 aactgtaaaa gaaacattcc taacatggtc attattagaa acgattattt ccgtttcagg
    26221 tatcatcttc attttattca tcagcttatt tgtttaacta aatattaagt aaacgtgttt
    26281 ttaggtaatt acaatatatt gcctcgaaac acgtttattt ttatgcagaa aatcgtctga
    26341 aatactcaga atccgaacga tataaaaata tattggttgt tttagtgtaa tgaaaggcgt
    26401 ttcaagacat gtttttgtgc gtattgtaca ggcgataatt atgaaacgtt tagtatgttg
    26461 ttttaaatta gataatgatg aatttaattt aaaaaataag tataaaaaat acaagccttg
    26521 tgtgacaagg gtttctgacg acttggatac aatttataag tatatttcaa ataataaaat
    26581 tatcaattaa cataaaatta atgacaatct taacttttca ttaactcgct tttttgtatt
    26641 gcttttaaaa accgaacaat atagacttgc atttattaag tttaaaaaaa ttaatgaatt
    26701 ttgcatttaa agggagatat tatagtgaaa aacaatctta ggtacggcat tagaaaacat
    26761 aaattgggag cagcatcagt attcttagga acaatgatcg ttgttgggat gggacaagat
    26821 aaagaagctg cagcatcaga acaaaagaca actacagtag aagaaaatgg gaattcagct
    26881 actgataata aagtaagcga aacacaaaca actacaacta acgttaatac tatagatgaa
    26941 acacaatcat acagcgcaac agcaacagaa caaccgtcaa acgcaacaca agtaacaact
    27001 gaagaagcac caaaagcagt acaagcgcca caaactgcac aaccagcaaa tgtagaaaca
    27061 gttaaagaag aggtagttaa ggaagaagcg aaccctcaag ttaaggaaac gacacaatct
    27121 caagacaata gcggagatca aagacaagta gatttaacac ctaaaaaggc tacacaaaat
    27181 caagtagcag aaacacaagt tgaagtggca cagccaagaa cggcattaga aagtaagcca
    27241 cgtgtgacaa gatcaacaga tgtagcggaa gctaaggaag ctagtgacgc gaaagtggaa
    27301 acgggtacag atgtgacaag taaagttaca gtggaagatg aaagtaaaat tgaagcgcca
    27361 aaaggaaata atgttcagcc tcatgaaggg caacgtgtag tattaaagta taaactgaaa
    27421 tttcaagatg ggttgaaaac aggcgattat tttgatttta cattatcaaa taatgtaaat
    27481 acacatggtg tagcaactac aagaaaagtt ccagatatta aaaatggatc attagttatg
    27541 gctaaaggtc aggtattaga taatggccga attagatata catttactga ttacatcaaa
    27601 gataaagtaa atgttacagc taatttagaa ataaacttat tcatagatcc taaaactgta
    27661 caaagcaatg gacaacaaac aataacttca aaattaaatg gcaaagaaac atcaggaact
    27721 atgcaaataa catataaaga tggtgttaaa aatcaatata caaatgtaaa tggttcgatt
    27781 gaaacgtttg acaaagaaaa aaataaattt acacacgtag catatattaa gcctataaat
    27841 ggaaacaatt cagatagtgt tactgtaacg ggtatgttga cacaaggaag taatgagaat
    27901 ggaacacaac caaatgttaa aatatatgag tatgttggag ttgaaaatgg gctaccacaa
    27961 agtgtttatg caaatacagt ggacagcaca caattgaaag atgtaacgaa tcaaatgggt
    28021 gataaattga aagtacaaaa caatggtagc tactcattaa attttgacaa attagataaa
    28081 acatatgtaa ttcattacac cggagattac ttaaatggta cgagtgaagt gaactttaga
    28141 actcaattaa ctggataccc tgaaaatcgc tataaaacat attattatta taacaatggc
    28201 tatacattaa catgggataa tggattagtt ttatatagta ataaagctaa tggtgatgga
    28261 aaatatggtc caatcgtaga ctccaataac tttgagttct ctgaagatag tggtaatggt
    28321 tcaatcagcg gacaatacga tgcgaagcaa attattgaaa cagaagaaaa tcaagacaat
    28381 acaccgcttg acattgatta ccacacagct atagatggtg aaggtggtta tgttgatgga
    28441 tacattgaaa caatagaaga aacggattca tcagctattg atatcgatta ccatactgct
    28501 gtggatagcg aagcgggtca cgttggagga tacactgagt cctctgagga atcaaatcca
    28561 attgactttg aagaatctac acatgaaaat tcaaaacatc acgctgatgt tgttgaatat
    28621 gaagaagata caaacccagg tggcggtcag gttactactg aatctaactt agttgaattt
    28681 gacgaagagt ctacaaaagg tattgtaact ggcgcagtga gcgaccatac aacagttgaa
    28741 gatacgaaag aatatacaac tgaaagtaat ctgattgaac tagtagatga actacctgaa
    28801 gaacatggtc aagcacaagg gccagtcgag gaaatcactg aaaacaatca tcatatttct
    28861 cattctggtt taggaactga aaatggtcac ggtaattatg gcgtgattga agaaatcgaa
    28921 gaaaatagcc atgttgatat taagagtgaa ttaggttacg aaggtggcca aaatagcggt
    28981 aatcagtcat tcgaggaaga cacagaagaa gacaaaccta agtatgaaca aggtggtaac
    29041 atcattgata tcgacttcga cagtgtgcca caaattcatg gattcaataa gcataatgaa
    29101 attattgaag aagatacaaa caaagataaa cctaattatc aattcggtgg acacaacagt
    29161 gttgattttg aagaagatac acttccaaaa gtaagcggtc aaaacgaagg tcaacaaacg
    29221 attgaagaag atacaacgcc gccaacacca ccgacaccag aggtaccaag tgagccaggc
    29281 acaccaacac cgccaacgcc agaagtgcca agtgagccag gtaagccaac accaccaaca
    29341 ccagaggtac cagctgaacc tggtaaacca gtaccacctg ctaaagaaga acctaaaaag
    29401 ccttctaaac cagtggaaca aggtaaagta gtaacacctg ttattgaaat taatgaaaag
    29461 gttaaagcag tggcaccaac taaacaaaaa caagctaaga aatctgaact acctgaaaca
    29521 ggtggagaag aatcaacaaa caaaggtatg ttgttcggcg gattattcag cattctaggt
    29581 ttagcgttat tacgcagaaa taaaaagaat cacaaagcat aatcaatcta aaattgacag
    29641 gtttatttca taaattatat gaagtaagcc tgttttttaa aattaaatca aatttcccaa
    29701 gaaataatta catactcaat gacactatga aggcgttcta attagtgtta aaatgacgtt
    29761 gatacataga tttaatactt aggaaaagga gcacattaac tttgaaaaaa ataaaaaagg
    29821 caatcattcc cgctgctggt ttagggacta gatttttacc agcaactaaa gcgatgccaa
    29881 aggaaatgct tcctatctta gataaaccga caatacaata tatcattgaa gaagctgcaa
    29941 gagctggaat tgaagatatt attatagtga caggtcgcca caaacgcgcg attgaagatc
    30001 attttgatag tcaaaaagaa ttagaaatgg tgttaaaaga aaaaggtaaa tctgaattac
    30061 tagaaaaagt tcagtattca acggaacttg cgaatatttt ttatgtaagg cagaaagaac
    30121 aaaaaggttt agggcatgcg attagttctg cgcgtcaatt tatcggcaat gaaccatttg
    30181 cggtattatt aggtgacgat attgttgaat ctgaagtgcc agcagtgaaa cagttgattg
    30241 acgtttatga agaaacgggg cactcagtta taggtgttca agaagttccg gaagcagata
    30301 ctcatcgtta tggaattatt gatccattaa ctaaaaatgg acgtcagtat gaagtgaaaa
    30361 agtttgttga aaagccagca caaggtacgg caccatcaaa cctagctatt atggggcgat
    30421 atgtattaac gcctgaaatt ttcgattatt taaaaacgca aaaagaaggc gcaggtaacg
    30481 aaattcagtt aacagatgcg attgagcgta tgaataatga taaccaagtg tatgcatatg
    30541 acttcgaagg cgaacgttat gacgttggag aaaaattagg cttcgttaaa actaccatag
    30601 aatatgcatt gaaagatgac agtatgcgag aagaattaac acgatttatt aaagaattag
    30661 gtttataaca ttaaaaagca taaatgattc tatattaatg caactttgta ttcgtacaag
    30721 gttgtatttt tttcgcaaac aattgcttgc tgaaagcgca ccattaaatc taaccaacac
    30781 actatcacaa acatttatgg atattttgta attttataaa agcaaacgta gaataatgcg
    30841 ataagtaata atgctatcgt taaaaataag aatgacaatg caataaaatc atattcgaag
    30901 catagaattg cagacattaa aaatactgca ctcaaactga tacatagcgc aaatatatat
    30961 gcaatttggg tcttgtgttt atctgcaatg gtcgtttgat ttataaaaga gattagtgtg
    31021 aaatctttag ttattgcgac ataaatgcct aatataacca tactgataca gaacacgata
    31081 gaaaatacga tgttgacggt cattgttgca ccttcccgat agaatataat gtactgtctt
    31141 ttagtgattg atgcgtttct tatatatgta tgtttctaaa aaacagtacg tttttatcaa
    31201 tttttaattt attagttgaa tgaatggggt tatgaaaaag gaagttttaa tcttttgtca
    31261 tgaaataaat gggatgaata tcacgactag aagtaatgtt acgaacagga gcgtataaat
    31321 ctagagacgc taaattcgac atagtatgtt gctcaattat ggctgatgat gaatttaaag
    31381 tatgtgcgtt ggaactgtcg gatttttgtt cataatgttt tgcatattgt gccatgatga
    31441 atagtgtaaa aataaataaa acaataagag atataatgcc cataatcaaa agtatttgtt
    31501 tagagccttt cattatttca catcctttct aaaatatatt tgtaactaaa tttaaaatag
    31561 ttatttttgt aattctaaac ctttttcatc gcgaaaacaa ttaaataggt cgcggtatta
    31621 attattatat tattaccgct taatatgaaa aatacatgaa aattaatttt ctaatatact
    31681 tttgaaaaat tattacaaat tagccccttc aaaacgcgaa aacataagga ttctagtttc
    31741 aaaagggctg ataagtataa aatgaaatgt aatatttcga tgtttaaaat ttttaattag
    31801 ctaaaaatca tcgcattaat tttttgagct acatcatcaa aatccggaca ttttaacgac
    31861 acatataatt taattttagg ttcagtacca gaaggacgta aagcgataaa tccttcgtca
    31921 aataagacac gaataacatt tgacttagga gaatcaatct gcgacgttgt atctttatcc
    31981 aactgataaa cctcgctagt taaataatct tcaattgctt tcactttcag tccttgaatc
    32041 tcttgcggtg gatttgaacg gaaatgtgtc ataattgatt caattttttt cttaccttct
    32101 aaaccttcta gcgtatgcga gaataatgta tcctcatgtc taccaacagt ttgataaatc
    32161 tgttctaatt catctttcaa tgttttgcca tataatttta actcagaagc gtattttata
    32221 atgagtggca caatttgtac ggcatcttta tcacgtacaa aaggctctga tagaaaaccg
    32281 taactctctt caaatgcgaa aatcatattt tgatgatcgt ccagttgtct tatttcctga
    32341 gcaataaatt taaagcccgt cagcacctct ttgtattcaa cattattata gcgtgctaat
    32401 gactttgtta actcactact tacaatagat tgaatcatta agcggtgacg taattgagac
    32461 gtttgttgaa tacgataatt aagtaacagc gcaccaattt ggttgccatt aaaatatgtg
    32521 atatgaccgt gtgcatcacg ttcagcaata ccaagacgat ccgcatcagg gtcagttgat
    32581 attaataagt ctgcatgact tttatttgca agttcgactg cttgatcaaa agcgcgatga
    32641 tcttcaggat tagcactttg aactgagcta aaattcggat caggtttaca ttgtgcttct
    32701 acaagattga attgattgaa attcaaagat tttaataact caggtacaat cggaacactt
    32761 gtaccatgca aactagtgaa cacaacttgt aaatcagatt taggaatata gccaatcata
    32821 ttttgaatgt gtttcatata gtcatcagtt acagatttcg ggaaaggctt gatataagat
    32881 gtattttgtt ttgaaatagg tatatcaatc tgtaatggat cgccaacttc ttcgatataa
    32941 cgacttacaa gctcagatgc atcggtcgat agttgcgcgc catcagaact atatacttta
    33001 atgccgttat agtctttcgg attatgactt gctgtaatca taatgccagc agtagtatta
    33061 agattgcgca ccgcgaacga taattccggt gtcgttttat atgtatcagg taaataaact
    33121 attatttgat ggtttgctaa tacattagca atgatttggg cgaattcagt tgaaagatgt
    33181 ctaatatcat aatgaatgac tattgttgga ttgtttgttt gggcatttaa ataacgcgct
    33241 aaacctaatg ccaatttttc aatagtaaac ttattaagtc gaccttcacc aagaccgaat
    33301 tttccgcgta tacccgcagt accaaacgtt aatttacttt caaaaccttc tcgctgttca
    33361 atgtcagatt gttgctcata aaaatgttta actaaactat cattagctct ttttatccat
    33421 aactctttat ccattgttgc taaacatcct ttcaaaatct cagttagact taataaaaca
    33481 tgaaaactaa agcccttaca tttatgtaat gaattataaa gaaatacgcc ccaaaagtaa
    33541 aaaaacacag ccccaagaca atacttttca caagtattat ataatagatg tgtatgaaaa
    33601 tgcatggagt agagcgttca tgtgaatgta aaatattaat ttttaatatt gatttatata
    33661 tagttttgta atttttgata gaataacaat taagatgatt ttaaattgaa gggtgagtct
    33721 tatgaaaaaa gtaatcaatg tagtaggagc tattattttt tctgataaca aaattctttg
    33781 tgcacagaga agtgaaaaaa tgagtctgcc tttaatgtgg gaatttcctg gcggtaaggt
    33841 tgaaaagaat gaaactgaaa aagacgcttt gattagagaa attagagaag aaatgaaatg
    33901 tgatctaatt gttggagaca aagttataac tacagaacat gaatatgatt ttggaattgt
    33961 taggttaaca acatacaaat gtactttaaa caaagagtta ccaactctaa ctgaacataa
    34021 gagtattgaa tggttgtcaa taaacgaact tgataaatta aattgggccc cagcggatat
    34081 accagccgtt aataaaatta tgactgaggg ataatatgag tagattacta aatgatttca
    34141 atcaatcttt acataaaggg tttatagaca aacatatttc tcataaaggg aattatacac
    34201 cgaagctttt agtaaataat aaaaatgaaa aagttctttc aacaattata gatgaacttc
    34261 aaaaatgtga aacgttctat ttttctgtgg cttttataac agaaagcggt ttagccagct
    34321 taaaggctca actattagat ttaagcaata agggtgttaa agggaaaata ttaacatcta
    34381 attacttagg gtttaatagt cctaaaatgt atggagaatt acttaaatta aaaaatgtag
    34441 aggtaagatt aaccgatatt gctggattcc atgccaaagg atatattttt gaacataagg
    34501 attatagttc tatggtaata ggaagctcta atttaacatc gaatgcgtta aaggttaatt
    34561 atgagcataa tgttttactg tctactatga aaaatgggga tttggttgat agtgtaaaaa
    34621 atgaatttga cttgttatgg caaaacagta cccaactgac tcagcaatgg attaattcat
    34681 ataaagaatc atttgagtac cgctccttag aaaaattggc ggaggttgaa caaactcaaa
    34741 tgctgctagc tgataaggtg aaaaaatcag tggaaattgt accgaattta atgcaagcag
    34801 aagcattaag gtcattaaaa gctattaggg ataaagccaa agataaggcg ttaataatat
    34861 ctgcaactgg tacaggtaaa acgattttat gtgcattaga tgttagagaa gtaaacccta
    34921 ataaattttt atttattgtt cataatgagg gaattttaaa tagagctaag gaagaattta
    34981 aaaaagtatt acctacaaaa aatgatagtg attttggatt gttgacaggc aaacatagag
    35041 atgtcgatgc caaatattta tttgcaacaa ttcaaacatt atctagagat gataatttta
    35101 agcagtttga tgaaaaagaa tttgattata ttgtttttga tgaagcacat cgttctgctg
    35161 catctactta tcaacgagta tttaattact tcaaacctaa gtttatgctg ggtatgactg
    35221 caacaccaga aagatcagat gaattaagta tatttgaaat gtttgattat aatattgctt
    35281 atgaaataag gttacaagca gcattagaaa gtgatatttt atgtccattt cattattttg
    35341 gtgtgactga ttatgtacat caaggtatta aagaagatga tgtaactaaa ctgagatatt
    35401 taacttctga tgaaagagtt aattatatta ttcaaaagac agattactat ggatattcag
    35461 gtgaaatttt acaaggatta atttttgtaa gtagcaaaaa agaagcttat gatttagcgg
    35521 ataaactaag ttcaaaaggg attaaaagtg ttgctttaac aggagacgat agtgttaatt
    35581 atcgccaaat tgtaatagaa aaattaagag aaggtaagat taactatatt ataactgtag
    35641 atctatttaa cgaaggtatt gatattccag aagtcaatca agttgtaatg ctgagaccaa
    35701 ctgagtctag tattatattc attcagcaac ttggtagagg gttaagaaaa agttcaaata
    35761 aagagtatgt tacagtaatt gattttattg gtaattataa gacaaattat ttaattccga
    35821 ttgcgctttc tggggatcaa tcgcaaaata aagataatta taagaagttt ttaacgaata
    35881 acgattcgat taatggagta tctacaatta attttgaaga agttgctaaa aaacagattt
    35941 acaattcatt ggatgcagta tcattgaatc aaaataaatt aatattaaaa gcttatgaag
    36001 aagttgaaaa tagattggga cacatgccgt tactaatgga ttttatacaa caacattcaa
    36061 tagatccaag cgttatattt tctaaattta gtaattatta cgaattttta
//
LOCUS       DAMZPP010000008        34898 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMZPP010000008 DAMZPP010000000
VERSION     DAMZPP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 34898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34898
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.008"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            127..1014
                     /gene="pdxS"
                     /locus_tag="POH73_000303"
     CDS             127..1014
                     /gene="pdxS"
                     /locus_tag="POH73_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDI6886287.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            1018..1578
                     /gene="pdxT"
                     /locus_tag="POH73_000304"
     CDS             1018..1578
                     /gene="pdxT"
                     /locus_tag="POH73_000304"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDI6886288.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(1785..2999)
                     /locus_tag="POH73_000305"
     CDS             complement(1785..2999)
                     /locus_tag="POH73_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDI6886289.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            3157..3618
                     /locus_tag="POH73_000306"
     CDS             3157..3618
                     /locus_tag="POH73_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDI6886290.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            3637..4203
                     /locus_tag="POH73_000307"
     CDS             3637..4203
                     /locus_tag="POH73_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDI6886291.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLVE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            4193..5200
                     /locus_tag="POH73_000308"
     CDS             4193..5200
                     /locus_tag="POH73_000308"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDI6886292.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            5214..7670
                     /locus_tag="POH73_000309"
     CDS             5214..7670
                     /locus_tag="POH73_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDI6886293.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            8154..9518
                     /gene="radA"
                     /locus_tag="POH73_000310"
     CDS             8154..9518
                     /gene="radA"
                     /locus_tag="POH73_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDI6886294.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELVGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSF
                     ALHS"
     gene            9543..10616
                     /locus_tag="POH73_000311"
     CDS             9543..10616
                     /locus_tag="POH73_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDI6886295.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIIGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            11173..12627
                     /locus_tag="POH73_000312"
     CDS             11173..12627
                     /locus_tag="POH73_000312"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDI6886296.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMEISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            13051..13698
                     /gene="cysE"
                     /locus_tag="POH73_000313"
     CDS             13051..13698
                     /gene="cysE"
                     /locus_tag="POH73_000313"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDI6886297.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            13682..15082
                     /locus_tag="POH73_000314"
     CDS             13682..15082
                     /locus_tag="POH73_000314"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDI6886298.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQTVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGA
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKKADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            15075..15479
                     /locus_tag="POH73_000315"
     CDS             15075..15479
                     /locus_tag="POH73_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDI6886299.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNEIITIVNER"
     gene            15487..16233
                     /gene="rlmB"
                     /locus_tag="POH73_000316"
     CDS             15487..16233
                     /gene="rlmB"
                     /locus_tag="POH73_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDI6886300.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLSTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            16233..16757
                     /locus_tag="POH73_000317"
     CDS             16233..16757
                     /locus_tag="POH73_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDI6886301.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDTIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            16838..17407
                     /locus_tag="POH73_000318"
     CDS             16838..17407
                     /locus_tag="POH73_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDI6886302.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSAEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRYDVENNYLNKLAIKELIRQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            17522..17665
                     /gene="rpmG"
                     /locus_tag="POH73_000319"
     CDS             17522..17665
                     /gene="rpmG"
                     /locus_tag="POH73_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDI6886303.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGTATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            17721..17903
                     /gene="secE"
                     /locus_tag="POH73_000320"
     CDS             17721..17903
                     /gene="secE"
                     /locus_tag="POH73_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDI6886304.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFG"
     gene            17916..18464
                     /gene="nusG"
                     /locus_tag="POH73_000321"
     CDS             17916..18464
                     /gene="nusG"
                     /locus_tag="POH73_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDI6886305.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            18645..19067
                     /gene="rplK"
                     /locus_tag="POH73_000322"
     CDS             18645..19067
                     /gene="rplK"
                     /locus_tag="POH73_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDI6886306.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            19275..19964
                     /gene="rplA"
                     /locus_tag="POH73_000323"
     CDS             19275..19964
                     /gene="rplA"
                     /locus_tag="POH73_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002020168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDI6886307.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASL"
     misc_feature    20055..20201
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            20239..20739
                     /locus_tag="POH73_000324"
     CDS             20239..20739
                     /locus_tag="POH73_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDI6886308.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     gene            20782..21150
                     /gene="rplL"
                     /locus_tag="POH73_000325"
     CDS             20782..21150
                     /gene="rplL"
                     /locus_tag="POH73_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDI6886309.1"
                     /translation="MANHEQIIETIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            21325..21933
                     /locus_tag="POH73_000326"
     CDS             21325..21933
                     /locus_tag="POH73_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6886310.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            22148..25699
                     /gene="rpoB"
                     /locus_tag="POH73_000327"
     CDS             22148..25699
                     /gene="rpoB"
                     /locus_tag="POH73_000327"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDI6886311.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEFNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            25863..29459
                     /gene="rpoC"
                     /locus_tag="POH73_000328"
     CDS             25863..29459
                     /gene="rpoC"
                     /locus_tag="POH73_000328"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDI6886312.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEVI
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            29596..29850
                     /locus_tag="POH73_000329"
     CDS             29596..29850
                     /locus_tag="POH73_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDI6886313.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            29948..30361
                     /gene="rpsL"
                     /locus_tag="POH73_000330"
     CDS             29948..30361
                     /gene="rpsL"
                     /locus_tag="POH73_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDI6886314.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            30427..30897
                     /gene="rpsG"
                     /locus_tag="POH73_000331"
     CDS             30427..30897
                     /gene="rpsG"
                     /locus_tag="POH73_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDI6886315.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            31020..33101
                     /gene="fusA"
                     /locus_tag="POH73_000332"
     CDS             31020..33101
                     /gene="fusA"
                     /locus_tag="POH73_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDI6886316.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKVAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            33318..34502
                     /gene="tuf"
                     /locus_tag="POH73_000333"
     CDS             33318..34502
                     /gene="tuf"
                     /locus_tag="POH73_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDI6886317.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
BASE COUNT        12563 a         5158 c         6886 g        10291 t
ORIGIN      
        1 tgatggtata aagtgttttt caattttata actgacctcc taaatttgtc ttattttgta
       61 cctttttaaa tatcagttta tacattacaa tgtatttaat caacttgaaa aggggtttta
      121 tgtataatga gtaaaattat tggatcagac agagtcaaaa gaggtatggc tgaaatgcaa
      181 aaaggcggcg ttattatgga tgtcgttaat gctgagcaag caagaattgc agaagaagct
      241 ggcgcggtag cagttatggc attagaacga gtaccttctg atattagagc tgctggtggc
      301 gttgcacgta tggcaaaccc taaaattgta gaagaagtaa tgaatgctgt ttctattcca
      361 gtcatggcta aagcacgtat tggtcatatc actgaagcaa gagtattaga ggcgatgggt
      421 gttgactata ttgatgaatc agaagtgtta acaccagcag atgaggaata tcacttaaga
      481 aaagatcaat ttacagtacc atttgtatgt ggatgtcgta atttaggtga agctgcgcgt
      541 agaattggtg aaggtgctgc tatgttacgt actaaaggtg aaccaggtac aggtaatatt
      601 gttgaagctg taagacatat gagacaagtt aattcagaag ttagtcgatt gactgtaatg
      661 aatgatgatg agattatgac tttcgcgaaa gatatcggtg cgccttatga aattttaaaa
      721 caaattaaag acaatggtcg tttaccggta gttaactttg cagctggtgg cgttgcgact
      781 cctcaagatg ctgctttaat gatggaatta ggtgccgatg gtgtattcgt tggatcaggt
      841 atttttaaat cagaagatcc agaaaaattt gctaaagcaa ttgttcaagc aacaacacat
      901 taccaagact atgaactaat tggaagatta gcaagtgaac ttggcactgc tatgaaaggt
      961 ttagatatca atcaattatc attagaagaa cgtatgcaag agcgtggttg gtaagatatg
     1021 aaaataggtg tattagcatt acaaggtgca gtacgtgaac atattagaca tattgaatta
     1081 agtggtcatg aaggtattgc agttaaaaaa gttgaacaat tagaagaaat cgagggctta
     1141 atattacctg gtggcgagtc tacaacgtta cgtcgattaa tgaatttata tggatttaaa
     1201 gaggctttac aaaattcaac tttacctatg tttggtacat gcgcaggatt aatagttcta
     1261 gcgcaagata tagttggtga agaaggatac cttaacaagt tgaatattac tgtacaacga
     1321 aactcattcg gtagacaagt tgacagcttt gaaacagaat tagatattaa aggtatcgct
     1381 acagatattg aaggtgtctt tataagagcg ccacatattg aaaaagtagg tcaaggcgta
     1441 gatatcctat gtaaggttaa tgagaaaatt gtagctgtcc agcaaggtaa atatttaggc
     1501 gtatcattcc atcctgaatt aacagatgac tatagagtaa ctgattactt tattaatcat
     1561 attgtaaaaa aagcatagct taatgtatgc taaatcaacg aattattgat atttatagat
     1621 ttgttgagaa gaaaattact ccttcaaact tagctttgga ggagttattt tttatgtcaa
     1681 aattaaaaat gataaaagat aaagatatac ataagaaaaa acccttcaaa gagactgaga
     1741 atagtcaaaa ttttgaaggg gttaattcga tgttgatgta tttgttaaat aaagaatcca
     1801 gcgattgcag ctgaaatgaa agatactagt gttgcaccga ataataattt caaaccaaag
     1861 cgggcaactg tatctccttt tttgtcatta agtgatttaa tcgcacctga aataatacca
     1921 atagagctaa agttagcaaa tgatactaag aatacagatg taacaccttt tgcgtgttca
     1981 gataaatcac taagtttacc aagtgcttgc attgctacaa attcgttaga taatagtttt
     2041 gttgccataa ctgaaccggc ttgaactgca tcttgccatg gcacacctac taagaatgca
     2101 aatggtgcaa agacaaaacc aattaatgtt tggaaatccc aagaaatagc gccacctgaa
     2161 actgtactaa agatattgct tacaattcca tttaatagag cgataatggc aatgtaaccg
     2221 attagcattg cgcctacaat gacagctact ttaaatccat ctaaaatata ttctcctagc
     2281 atttcaaaga atgattgttg tctttcttca gtttcttcaa ctaataattt gtcatcttct
     2341 tcattaactt tataagggtt aataattgaa gcgatgataa aaccaccaaa taagtttaag
     2401 acaacagccg ttacaacata tttaggttca attaaggtaa agtatgcacc gataattgaa
     2461 gcagaaacag tcgacattgc tgaagctgtt aatgtgtata aacgttgctt aggtatgtat
     2521 ggtaattgtt ttttaattga aataaatact tcagattgtc ccaaaattgc tgcagcaact
     2581 gcattgtatg attctaaacg tcccatacca ttaattttag aaattaagaa tcctaaaaca
     2641 ttaatgatta aaggtaaaat ctttgtgtat tgaaggatac cgataatcgc tgaaataaat
     2701 acgataggta ataatacact gaagaagaat ggtggttgct taggatcgat atattgaata
     2761 ccaccgaata caaagttaac accatctgca gcttttaata ataagtagtt aaaaccgttc
     2821 gaaataccac caataacctt gattcccatt gtagttttaa gcaagataaa tgcaaagata
     2881 agctgaattg caagtaaaat tcctacatat ttccagcgaa tatttttcct gtctgagcta
     2941 aatagaaacg caagtgctaa aaagaagata attccgataa tcccaattag aatatgcata
     3001 tatttctcat tcctttagtt ttttctacaa tctatcatac aataaaatgg aagggctaac
     3061 atcataaatt tttgaaaata taaaaacaaa ttaattgaaa aaggtcaaaa taggtcatat
     3121 aatatagtca aagaaggtca aaaaggggtg atatacatgc acaatatgtc tgacatcata
     3181 gaacaataca tcaaacgttt atttgaagag tcgaatgagg atgtcgttga aattcagaga
     3241 gcgaatatcg cacagcgttt tgattgcgta ccatcacagt taaactatgt aatcaaaaca
     3301 cgattcacta atgaacatgg ttatgaaatc gaaagtaaac gtggtggtgg tggttacatc
     3361 cgaatcacta aaattgaaaa taaagatgca acaggttata ttaatcattt gcttcagctg
     3421 attggacctt ctatttctca acaacaagct tattatatta ttgatgggct tttagataaa
     3481 atgttaataa atgaacgtga agctaaaatg attcaagcag ttattgatag agaaacgcta
     3541 tcaatggata tggtttctag agatattatt agagcaaata ttttaaaacg tttgttacca
     3601 gttataaatt attactaaat gaaatgaggt gttgaagtgc tttgtgaaaa ttgtcaactt
     3661 aatgaagcgg aattaaaagt taaagttaca agtaaaaata aaacagaaga aaaaatggtg
     3721 tgtcaaactt gtgctgaggg gcaccatccg tggaatcaag ctaatgaaca acctgaatat
     3781 caagaacatc aagataattt cgaagaagca tttgttgtta agcaaatttt acaacattta
     3841 gctacgaaac atggcatcaa ttttcaagaa gtagcgttta aagaagaaaa acgttgccca
     3901 tcatgtcaca tgactttgaa agatattgca catgttggta aatttgggtg tgctaattgt
     3961 tatgcaacat ttaaagatga catcattgat atcgtccgca gagttcaagg tggacaattt
     4021 gagcacgttg gaaagacacc acattcttca cataaaaaga tagctttaaa gcgaaaaatc
     4081 gaagaaaaga atgaatattt gaaaaaactt gttgaaatcc aagattttga ggaagcagcc
     4141 attgttagag atgaaattaa agcactaaaa gctgagagtg aggtgcaaca tgatgacgca
     4201 taatattcat gataatatca gccaatggat gaaaagtaat gaagaaacac caattgttat
     4261 gtcttctaga attcggttag cgcgtaattt agaaaatcat gtgcatccac taatgtatgc
     4321 tactgaaaat gatggattta gagttataaa tgaggtacaa gatgccttgc caaattttga
     4381 attaatgcgt cttgatcaaa tggatcaaca aagtaaaatg aaaatggttg caaagcattt
     4441 gattagccct gaactaataa aacaaccagc agccgcagta ttagtgaatg atgatgaatc
     4501 tttaagtgtc atgataaatg aagaggacca tattcgtatt caagctatgg gaactgacac
     4561 gacattacag gctttatata atcaagcttc atcaattgat gatgaattag atcgaagcct
     4621 tgatataagt tatgatgaac aacttggtta tttaactaca tgtcctacca atataggtac
     4681 tggtatgaga gcaagcgtga tgctacattt accaggtcta tctattatga aaagaatgac
     4741 acggattgct caaaccatta atcgttttgg atatacaatc agaggtattt acggtgaagg
     4801 ttcgcaagtt tatggacata cttatcaagt atccaaccaa cttacacttg gtaaatctga
     4861 gttagaaatc atagaaacat taacagaagt tgttaatcaa atcattcatg aagaaaaaca
     4921 aatacgacaa aagttagaca cttataatca actagaaaca caagaccgtg tttttcgctc
     4981 gctaggtatt ttacaaaact gtagaatgat aactatggaa gaggcttctt atagattaag
     5041 cgaagttaaa cttggtatag atttaaatta cattgaatta caaaacttta aatttaatga
     5101 attgatggta gctatacagt caccattttt attagatgaa gaagatgaca aatctgtaaa
     5161 agaaaaacga gcagatatac taagagaaca tataaagtag gaggtcatta tttatgttat
     5221 ttggtagatt aactgaacgt gcacagcgcg tattagcaca tgcacaagaa gaagcaattc
     5281 gtttaaatca ttcaaatata ggaacagaac acctattatt ggggttaatg aaagaacctg
     5341 aaggaattgc tgcaaaagta ttagaaagtt ttaatatcac tgaagataaa gtaattgaag
     5401 aagttgaaaa attaatcgga catggtcaag atcatgttgg tacattgcat tatacaccta
     5461 gagctaaaaa agtcattgaa ttatcgatgg atgaagctag aaaattacat cacaattttg
     5521 ttggaacgga acatatttta ttaggcttga ttcgtgagaa tgaaggtgtt gcagcaagag
     5581 tttttgcaaa tctagattta aatattacta aagcacgtgc acaagttgtg aaagctttag
     5641 gaaaccctga aatgagtaat aaaaatgcac aagctagtaa gtcaaataat actccaactt
     5701 tagatagttt agctcgtgac ttaacagtca ttgccaaaga cggtacatta gatcctgtta
     5761 taggacgtga taaagaaatt acacgtgtaa ttgaagtatt aagtagacgt acgaaaaaca
     5821 atcctgtgct tattggagag ccaggtgttg gtaaaactgc tattgctgaa ggtttagcgc
     5881 aagccatagt gaataatgag gtaccagaga cattaaaaga taagcgtgtt atgtctttag
     5941 atatgggaac agtagttgca ggtactaaat atcgtggtga atttgaagag cgtctgaaaa
     6001 aggttatgga agaaatccaa caagcaggta atgtcatcct atttattgat gaattgcata
     6061 ctttagttgg tgctggtggt gctgaaggtg ctatcgatgc ttcgaatatt ttgaaaccgg
     6121 cattagcacg tggtgaatta caatgtattg gtgctactac attagatgaa tatcgcaaaa
     6181 atattgaaaa agacgcggct ttagaacgtc gtttccaacc tgtacaagtt gatgaacctt
     6241 cagtagtaga tacagttgct attttaaaag gattaagaga tcgttacgaa gcacaccatc
     6301 gtattaatat ttcagacgaa gctattgaag cagctgttaa attaagtaac agatatgttt
     6361 cagatcgttt cttaccagat aaagcaattg atttaattga tgaagcaagt tctaaagtaa
     6421 gacttaagag tcatacgaca cctaataatt taaaagaaat tgaacaagaa attgaaaaag
     6481 ttaaaaatga aaaagatgcc gcagtacatg ctcaagagtt tgaaaatgct gctaatctgc
     6541 gtgataaaca aacaaaactt gaaaagcaat atgaagaagc taaaaatgaa tggaagaacg
     6601 cacaaaatgg catgtcaact tcattgtcag aagaagatat tgctgaagtc attgcaggat
     6661 ggacaggtat cccattaact aaaatcaacg aaacagaatc tgaaaaactt cttagtctag
     6721 aagatacatt acatgagaga gttattgggc aaaaagatgc tgttaattca atcagtaaag
     6781 cggttagacg tgcccgtgca gggttaaaag atcctaaacg accaattggt agctttatct
     6841 tccttggacc aactggtgtt ggtaaaactg aattagctag agctttagct gaatcaatgt
     6901 ttggcgatga tgatgcgatg atccgtgtag acatgagtga atttatggaa aaacacgcag
     6961 tgagccgatt agttggtgct cctccaggat atgttggtca tgatgatggt ggacaattaa
     7021 ctgaaaaagt tagacgtaaa ccatattctg taattttatt tgatgaaatt gaaaaagcac
     7081 atccagatgt atttaatatt ctattacaag ttttagatga cggacatttg acagatacaa
     7141 aaggacgtac agttgatttc agaaatacaa ttatcataat gacatcaaac gttggagcac
     7201 aagaattaca agatcaacga tttgctggat tcggtggttc aagtgatgga caagattatg
     7261 aaacaattcg aaaaacgatg ttaaaagaat taaaaaattc attccgtcca gaatttttaa
     7321 accgtgtaga tgatatcatt gtattccata aactaacaaa agaagaatta aaagaaattg
     7381 taactatgat ggttaataaa ttaacaaatc gattatccga acaaaacata aatattattg
     7441 ttactgataa agcgaaagat aaaatcgcag aagaaggata tgatccagaa tatggtgcaa
     7501 gaccattaat tagagcgata caaaaaacta tcgaagataa tttaagtgaa ttaatattag
     7561 atggtaatca aattgaaggt aagaaagtta cagtagatca tgatggtaaa gagtttaaat
     7621 atgacattgc tgaacaaact tcagaaacta aaacaccatc gcaagcataa ttataaaaca
     7681 gtccaaaaca aattaaagtt ttgggctgtt ttttagtagc attgaactat agaaattcgt
     7741 gaaagtatcc accaacgaaa caacctaata aaataatcat caaaggatag ttaagaatta
     7801 tatgaaacaa gttaatgagc atacagcgat agtaaaaagg tatgaatttt tataaaggtt
     7861 ttttgtttga agatactagc aacttacgtc aaaataaaat aggtctatat aatattgtga
     7921 taaatctaga gaaaagagtt tactaagaaa ttattaagat tttatctttg aaagatacct
     7981 actatcaaca tgatgtaagt ttattttata atattcataa aaaataaata tgataaaaca
     8041 gtttgcgtta gtagtcatgt taaaatacaa acaaatgtaa ctcacattta atttgtcata
     8101 atgggaatgt gcgtttaaaa tagatttgtt caaaggaaag tggaggtgca attttggcca
     8161 agaaaaaagt gatttttgaa tgtatggctt gtggttatca atctcctaaa tggatgggga
     8221 aatgtcctaa ttgtggcgct tggaatcaaa tggaggaaat tgttgaaaaa gcagccaatc
     8281 ctaaacatgg agtgaaaacc aaggaattag taggtaaagt acaaaaatta aatagtatta
     8341 aacatgaaac aacgccgaga gtgttaacag attcagcaga atttaaccgt gtattaggtg
     8401 gaggtattgt gagcggatcg ttagtactta ttggtgggga tccaggtatt ggtaagtcaa
     8461 cgttactttt acaaatttgt gcatcgttat ctcaaaagaa aaaagtacta tatattactg
     8521 gagaagaatc gcttagtcag actaaattac gtgcagagcg attagatgaa gattcaagtg
     8581 aattgcaagt attagctgaa acagatcttg aagttattta tcaaacagta aaagaggaac
     8641 aacctgattt actagtagtg gattcgattc aaacaatata tcatcctgaa atcagctctg
     8701 cgccaggttc tgtttcacaa gttcgtgaaa gtacacaaag tttaatgaat attgctaaac
     8761 aaatgaacat tgcaactttt atagtgggtc atgtaacgaa agaaggtcaa attgctggcc
     8821 caagattgct agaacacatg gttgatactg tactttattt tgaaggcgat gaacaccacg
     8881 catatcgaat tttgcgagct gttaaaaacc gttttggttc aacgaatgaa atgggaatct
     8941 tcgaaatgaa gcaaagtgga ttaaaaggtg taaataatcc atctgaaatg tttttagaag
     9001 aacgttcaac aaatgttcca ggttcaacaa ttgttgcaac catggaggga actagaccac
     9061 ttttaataga agttcaagcg ctggtaactc caacgacttt taacaatccg agacgaatgg
     9121 caacaggaat tgatcacaat cgattaagtt tgttgatggc tgttttggaa aagaaagaaa
     9181 attatctatt acaacaacaa gatgcttata tcaaagtagc tggcggtgta aagttaacgg
     9241 agccagcagt tgatttaagt gtaattgtag caactgcgtc tagctttaaa gataaagctg
     9301 tcgacggatt agattgctat attggagaag ttggtttaac aggtgaggta cgtcgtgtat
     9361 ctcggataga acaacgcgtg caagaggctg caaaactagg tttcaaacgt gtaattattc
     9421 ctaaaaataa tataggcgga tggacatatc ctgaaggtat acaagtaata ggtgtaacta
     9481 ctgtacatga agcattgtcg tttgctcttc attcataaaa catcaagaaa ggaggacatt
     9541 gtgtgaatat cgttaaacta atggttatta ttatttactt aattattggg agcgcattag
     9601 gaataattat tattcctgaa attgcaaatg atcttggatt acaaaactcc agctttttaa
     9661 aaaatcacta tgtagatggc attatcggta gtatttttat gttcttaatt tttggtgtat
     9721 ttattagacg agttactaac gctataatag gtttagaaca ttttattatg cgtagaagtg
     9781 ctgttgaaat actattcgca acaataggtt taataatcgg attacttatt tctgttatgg
     9841 tgtcgtttat attggaatca attggcaact ctatttttaa tcatttcatt cctgtcataa
     9901 ttacgatatt actatgttat ttcggtttcc aatttggcct taaaaaacga gatgaaatgt
     9961 taatgttttt acctgagaat atagcgcgtt ccatgtcaca acatactaaa agtgctacac
    10021 caaaaattat cgacacaagc gcaattattg atggtcgtat tttagaagtc attcgttgcg
    10081 gttttatcga tggcaatatt ttaattccac aaggtgttat taatgaatta caaattgttg
    10141 cagattcaaa tgacagtgtt aaacgtgaaa agggtaaaag aggcttagat atcttaaatg
    10201 aattgtatga tttagactat cctacaaagg ttatacatcc aactaaaaca catagtgata
    10261 ttgatacaat gctattaaaa ctagcaaaac aatatcatgc aagtattatt acaacagatt
    10321 tcaacctaaa taaagtatgt catgtacatg gtatcaaagc attaaatgtt aatgatttat
    10381 cagaagcaat caaacctaat gtacatcaag gtgatcaact tcatatttta ctgacaaaaa
    10441 tgggtaaaga gcctggtcaa gcagtaggat atctagatga tggtacgatg gtagttgttg
    10501 ataatgctaa aaatcttatt ggcagtcatg tcaatttaga agtagtgagc ttattgcaaa
    10561 catcttcagg aagaattgtt tttgctaaaa aaatcgaaga tacagtatca ttataaaaac
    10621 tgtagttgac ggaaacaaaa tagatattaa aaggtatatg aaatggcgaa gtttgtaatt
    10681 tgaagtataa ctataaataa atacaaaaca tatcagttat caaaaaatac aaatagtgct
    10741 aacatatcat tgaaacaagt tcattaagtg ttaaatttta gatattaata aagttattct
    10801 tatagcaatc aagacataca aaattagaat caattttaac gtatttgaat agttaatttg
    10861 aagtttaatt ttacagtaca taatattcat ttttaagtcg ttgaaatgcg tatgtagtgc
    10921 agtcttgatt cttagggata gcttttttaa agtgttgaaa aaacaactgc tttctattta
    10981 aaatttaagc atattaccca gggaattatg ttatgattaa acaatgaaat tattttaagg
    11041 tcatgttaaa aagcattgtt tcgttttaac aataagacga gttacacatt tgttatgacg
    11101 gtcgaagata gcaaatgaac aatttatatg aactaacttg aaaatctgat atttaattta
    11161 ggagtgaaaa aaatgagcga tcgtataaga gtaagatatg caccaagtcc aactggttat
    11221 cttcatattg gtaatgcaag aacagcatta ttcaattact tgtatgctaa acattacaac
    11281 ggagattttg tgattcgaat tgaagatact gataaaaaac gtaatttaga agatggagaa
    11341 acatcacaat ttgataatct taaatggtta ggattagatt gggatgagtc tgtagataaa
    11401 gacaatggct acggaccata tcgtcaatct gaacgtcaac atatctacca accattaata
    11461 gatcagttac tagcagaaga taaagcatat aaatgctata tgacagaaga agaattagaa
    11521 gctgaacgtg aagcgcaaat cgctcgtggt gaaatgcctc gctatggtgg tcaacatgcg
    11581 catttgactg aagaacaacg tcaacaattt gaagcagaag gacgccaacc atcaattcgt
    11641 ttccgagtac ctcaaaatca aacgtattca tttgatgata tggttaaagg gaatatttca
    11701 tttgattcaa atggtattgg tgactgggtt atcgttaaaa aagatggcat tccaacgtac
    11761 aattttgcag tagctataga tgatcattac atggaaattt cagatgtcat tcgtggtgat
    11821 gatcatattt caaacacgcc taaacaaatt atgatttatg aagcatttgg ctgggagcca
    11881 cctcgttttg gtcatatgtc attaattgtt aatgaagaac gtaaaaagtt aagtaaacgt
    11941 gatgggcaaa ttttacaatt tattgagcaa tatcgtgact taggttattt acctgaagcg
    12001 ttatttaatt ttattgcgtt attaggttgg tctcctgaag gtgaagaaga aatcttttct
    12061 aaagaagaat ttatcaaaat ctttgatgaa aagcgtttat caaaatcacc agcatttttc
    12121 gataagcaaa aattagcatg ggttaataac caatatatga aacaaaaaga tactgaaaca
    12181 gtattccaat tagcattacc tcatttaatt aaagcgaatt tgattcctga ggtgccgtca
    12241 gaagaggatt tatcttgggg acgcaaatta attgcgcttt atcaaaaaga aatgagttat
    12301 gccggtgaaa ttgtaccttt atcagaaatg ttctttaaag aaatgccagc tcttggtgag
    12361 gaagaacaac aagtgatcaa tggagagcaa gtaccagagt taatgacgca cttattcagt
    12421 aaattagaag cacttgaacc atttgaagcg gctgaaatta aaaagacaat taaagaagtt
    12481 caaaaagaaa caggaataaa aggcaagcaa ttatttatgc ctattcgtgt tgctgtaaca
    12541 ggccaaatgc atggtcctga attaccaaat acaattgaag tacttggtaa agaaaaagtg
    12601 ctaaaccgtt taaaacaata taagtaatga aatagacaag atcaaggtag tatatacttg
    12661 aattttataa cgtaaccaca tatgataaag ttgtattaac aaattacaac aatatgaata
    12721 gagtacttta tataaaacga aggattagta attaaattta tacgatgcag agagtgtacg
    12781 gttgctgtga gtacaacgta gaaattaatg aatgcacctt cgtaaaatga attaaatata
    12841 taatgagagt gatgagcatt aagttgactt agtttccttg ataatttgga agcgcccgca
    12901 attttattaa tgtaattcgc taaattcaga gtggaaccgt gcggaagcgc ctctaacaat
    12961 acaatttgta tgttagtggt gctttttttg atatttaatt tcgcaggtac ttcaattatg
    13021 tatttacgta gttcaatcat tgaggaggaa atgatcttgt taaaaagaat gagagacgat
    13081 ataaaaatgg tatttgagca ggatccagcg gcacgttcaa cattagaagt cattacaacg
    13141 tatgcaggtt tacatgcagt ttggagtcat ttgattgcac ataagttata caaccaaaaa
    13201 aaatatgttg cagcacgcgc gatatctcaa atttcaagat ttttcacagg tatagaaatc
    13261 catccaggtg ctaaaattgg aaagcgtcta tttatagatc atggtatggg cgttgtaata
    13321 ggagaaacat gtacaattgg tgataatgtg acaatctatc aaggcgtgac acttggtggg
    13381 acagggaaag aaagagggaa aagacaccca gatataggag acaatgtttt aatagcagcg
    13441 ggtgcgaaag ttttaggaaa tattaaaata aattcaaatg taaatattgg tgcaaattca
    13501 gttgttttac aatcagttcc aagttattca acggttgttg gtataccagg acatattgtt
    13561 aagcaagatg gtgttcgagt tggaaaaaca tttgatcatc gccatctacc tgatccaatt
    13621 tatgaacaaa ttaagcattt agaacgacaa cttgaaaaga ctaggaatgg agagattcaa
    13681 gatgattaca ttatataata cgcttacacg tcaaaaagaa gtgttcaagc caatagaacc
    13741 aggaaaggta aaaatgtatg tatgtggtcc tacggtatat aactacattc atattggtaa
    13801 tgcaagacca gcaattaatt atgacgtagt gagacgttac tttgaatacc aaggatataa
    13861 tgtagaatat gtatcaaatt ttacagatgt agatgataaa ttaattaaac gttctcaaga
    13921 attaaatcag actgttcccg aaattgcaga aaaatatatc gctgcttttc atgaagatgt
    13981 tggtgcttta aatgttagaa aagcgacttc aaatccaagg gtaatggacc atatggatga
    14041 cattattcaa tttattaaag atttggttga tcaaggttat gcatatgaaa gtggtggcga
    14101 tgtttacttt agaacacgta aatttgaagg ttatggtaaa ttaagtcatc aatccataga
    14161 tgacttaaaa gtgggtgctc gtatagatgc aggagagcat aaggaagacg cacttgattt
    14221 tacattgtgg aaaaaagcga agcctggcga gattagttgg gatagcccat ttggtgaagg
    14281 gagaccagga tggcatattg aatgttctgt aatggcattt catgagctag gagctacaat
    14341 tgatatacat gcgggtggtt cagatttaca atttccacat catgaaaatg aaatagcaca
    14401 atcggaagca cataatcatg cgccattcgc taattattgg atgcataatg gttttattaa
    14461 tattgataat gaaaaaatga gtaaatcact aggcaacttt attttagttc acgatattat
    14521 taaagaagtt gatccagatg tattaagatt ctttatgatt agcgtacatt atagaagtcc
    14581 aattaactat aatctagaat tggttgaatc agcacgtagt ggactagagc gtattcgcaa
    14641 tagttatcaa ttaattgaag agcgcgcaca aattgctact aatattgaaa atcaacagac
    14701 atatattgat caaattgatg cgattttaaa tcgttttgaa acagttatga atgatgattt
    14761 taatacagct aatgcaatta cagcttggta tgatttagca aaacttgcaa ataaatatgt
    14821 actagagaac acaacatcaa cagaagtaat tgataaattt aaagcagttt atcaaatttt
    14881 cagcgatgtt ttaggtgtac cgttaaaatc taaaaaagca gatgaattat tagatgaaga
    14941 tgttgaaaaa ttaatcgaag agcgtaatga agcaaggaaa aacaaagatt ttgcacgagc
    15001 agatgagatt cgagacatgc tgaaatcaca aaacattata ttagaagaca cacctcaagg
    15061 ggttagattt aaacgtggat aatcaacaag ataatcacat taaattattg aatccattga
    15121 ccttagcata tatgggagac gcagtcttag atcaatatgt acgtacgtat atcgttttaa
    15181 agcttaaaag taagcctaat aaactacatc aaatgtctaa aaaatatgta tctgccaaaa
    15241 gtcaggcgca aacgttagaa tatttaatgg agcaagaatg gtttacagac gaagaaatgg
    15301 atattttgaa acgagggcgt aacgcgaaaa gtcatactaa agctaaaaat actgatgttc
    15361 aaacatatcg taaaagttca gcgatagaag cagtgatagg ttttctttat ttagaaaaaa
    15421 gagaagaacg attagaggca ttattaaatg aaataataac aatagtaaac gaaaggtagt
    15481 gacaatgtgg aagatacggt tattgttggt aggcatgctg ttagagaagc gattattact
    15541 gggcatccga taaataagat attgattcaa gaaggaatta aaaagcaaca aattaatgaa
    15601 attttaaaaa atgcaaaaga tcaaaaaatc attgttcaaa ctgtaccaaa atctaaatta
    15661 gattttttag caaatgcacc acatcagggg gttgcagcgc ttattgcacc atatgaatat
    15721 gctgacttcg atcaattttt aaaacagcaa aaagaaaaag aaggtttatc gacagtgctt
    15781 atattagacg gcttagaaga cccgcataac ttgggatcaa ttttaagaac agcagatgca
    15841 acgggagttg atggtgttat tattcctaaa cgtcgttcag ttacactaac gcaaacagtt
    15901 gcaaaagcct caacaggtgc aattgaacat gtaccagtta ttcgagtgac aaatttagct
    15961 aaaactatcg atgaactaaa agataatggt ttttgggtag ctggcactga agctaataat
    16021 gcaacagatt atagaaatct tgaagcggac atgtcattgg ctattgtaat tggcagcgaa
    16081 ggacagggta tgagtcgtct agtaagtgat aaatgcgatt tttatattaa gattccaatg
    16141 gttggccatg taaacagttt gaatgcttcg gttgcagcaa gtttaatgat gtacgaagta
    16201 tttcgaaaaa gacatgatgt tggagaaata taatgaaaga acgttactta atcattgatg
    16261 gatacaatat gataggacaa tcaccaacgc taagcgccat tgcaaaagag aatttagaag
    16321 aagctagaat gcaattaata gatacaattg caaattataa tgcagttatt tcagatgaaa
    16381 ttatttgtgt tttcgatgct tatgaccaat cgggtgttga aagagaatac atgtatcatg
    16441 gcgttaaaac aatttttacc aaggaaaaag aaacagctga tagtttcata gaacgttatg
    16501 tctatgaact ttatgacaag cacactaagc atattacagt tgtaacaagt gatatgagtg
    16561 agcaacatgc tatctttgga tcaggtgcat atagaatatc atctcgcgaa atgtggagag
    16621 atttaaaaga aaatgaaatt gatgtgagta aatcattaga tgatataagt gaaaacaagc
    16681 caagaactcg aattccgtta tcttctgaaa tccttgcaga atttgaaaaa atacgaagag
    16741 gacatcataa gaaatgacat ttccatcatc ttgaaatttt gaatgtaaca atattaaact
    16801 aaccttaaat tttagataga aggggttagt ttaatacttg aaatatgatt tgacaactca
    16861 agacagtaca atcaaacgta acaatgcaat aaatgataaa gacttcgaaa agttagtaat
    16921 ggatctacaa ccattaatta tacggcgcat caaaacattt ggatttaatc attatgattt
    16981 agaagattta tatcaagaaa tacttatacg gatgtatagg tcggtccaaa catttgattt
    17041 tagtgcagag cagcctttca caaattatgt tcaatgttta attacgtctg taaagtatga
    17101 ttatttgaga aaatatttag ctacaaataa aagaatggat aatttgatta atgaatatag
    17161 agttacgtat ccatgtgcaa taaagcgtta tgatgttgaa aacaattatt tgaataaatt
    17221 agcaattaaa gagttgattc gtcagtttaa gtatttgagt gcatttgaaa aagatgtcat
    17281 gtatttaatg tgtgaacaat ataagccgag agaaattgct caactgatgc atgtaaaaga
    17341 gaaagtgatt tataatgcca tacaacgatg taaaaataaa ataaaacgtt atttcaaaat
    17401 gatttgaaaa gcgtcttagg acgtgaattg aattagaacg tgttacttcc tgatggtttg
    17461 acatttgtta taaattttat gtatagtata ctggtattat aatgaataaa ggtgaattat
    17521 tgtgaggaaa atacctttaa attgtgaagc ttgtggcaat agaaattata atgttcctaa
    17581 gcaagaaggc acggcaacaa gattaacctt aaagaaatat tgtccaaaat gtaacgcgca
    17641 cacaattcat aaagaatcga aataaataca ttcgaaataa tactttgata atatgttcaa
    17701 aggatttgga ggttgagcag atggctaaaa aagaaagttt ctttaaaggc gttaagtctg
    17761 aaatggaaaa aacaagttgg ccgacgaaag aagagctatt taaatatact gtaattgtag
    17821 tttctactgt tatattcttc ttagtctttt tctatgcctt agatttagga attacagcat
    17881 tgaaaaattt attatttggt tagaggagtg aagacatgtc tgaagaagtt ggcgcaaagc
    17941 gttggtatgc agtgcataca tattctggat atgaaaataa agttaaaaag aatttagaaa
    18001 aaagagtaga atctatgaat atgactgaac aaatctttag agtagtcata ccggaagaag
    18061 aagaaactca agtaaaagat ggcaaagcta aaacgactgt taaaaaaaca ttccctggat
    18121 atgttttagt ggaattaatc atgacagatg aatcatggta tgtggtaaga aatacaccag
    18181 gcgttactgg ttttgtaggt tctgcaggtg cagggtctaa gccaaatcca ttgttaccag
    18241 aagaagttcg cttcatctta aaacaaatgg gtcttaaaga aaagactatc gatgttgaac
    18301 tcgaagttgg cgagcaagtt cgtattaaat caggtccatt tgcgaatcaa gttggtgaag
    18361 ttcaagaaat tgaaacagat aagtttaaac taacagtatt agtagatatg tttggccgag
    18421 aaacaccagt agaagttgaa ttcgatcaaa tagaaaagct ttaattaaca attaaagtta
    18481 ttaaactaac caaaagataa aaaagagtat tgatttttta attagaaaag tgttaaaatt
    18541 atgtggtcgc gcttttagag cgcccatttc gtcacgaaat gttaagagtg ggagggcaaa
    18601 actgagccct gtgaccacat cacgatatca aggaggtgca catcgtggct aaaaaagtag
    18661 ataaagttgt taaattacaa attcctgcag gtaaagcgaa tccagcacca ccagttggtc
    18721 cagcattagg tcaagcaggt gtgaacatca tgggattctg taaagagttc aatgcacgta
    18781 ctcaagatca agcaggttta attattccgg tagaaatcag tgtttatgaa gatcgttcat
    18841 ttacatttat tacaaaaact ccaccggctc cagtattact taaaaaagca gctggtattg
    18901 aaaaaggttc aggtgaacca aacaaaacta aagttgctac agtaactaaa gatcaagtac
    18961 gcgaaattgc taacagcaaa atgcaagact taaacgctgc tgacgaagaa gcagctatgc
    19021 gtattatcga aggtactgca cgtagtatgg gtatcgttgt agaataattt tacgaatatt
    19081 aaatttgatt acatgattta aacgatgaag cagataacag agataataat gatgaattat
    19141 aaatataatc tgaatgacta gattaatgat tgatttattc atatgattaa ttcttctgtt
    19201 gtctgctctt aacttgcatc tagcaagtaa tgtgggagga aattccgcta aaaccactaa
    19261 aggaggaact ataaatggct aaaaaaggta aaaagtatca agaagcagct agtaaagttg
    19321 accgtactca gcactacagt gttgaagaag caattaaatt agctaaagaa acaagcattg
    19381 ctaactttga cgcttctgtt gaagttgcat tccgtttagg aattgataca cgtaaaaatg
    19441 accaacaaat ccgtggtgca gttgtattac caaacggaac tggtaaatca caaagtgtat
    19501 tagtattcgc taaaggtgac aaaattgctg aagctgaagc agcaggtgct gactatgtag
    19561 gtgaagcaga atacgttcaa aaaatccaac aaggttggtt cgacttcgat gtagtagttg
    19621 ctacaccaga catgatgggt gaagttggta aattaggtcg tgtattagga ccaaaaggtt
    19681 taatgccaaa ccctaaaact ggaactgtaa caatggatgt taaaaaagct gttgaagaaa
    19741 tcaaagctgg taaagtagaa taccgtgctg aaaaagctgg tatcgtacat gcatcaattg
    19801 gtaaagtttc atttactgat gaacaattaa ttgaaaactt caatacttta caagatgtat
    19861 tagctaaagc taaaccatca tctgctaaag gtacatactt caaatctgtt gctgtaacta
    19921 caacaatggg tcctggagtt aaaattgata ctgcaagttt ataataataa atgatataaa
    19981 caattacagg ctgaaagaaa tatctttcag tctgtaaaaa tatattgaca ataagtaatt
    20041 tccaagttat attacttatt gtgattattt tacctaagac agtaggagtt atttataact
    20101 taaaatttat cctgccgagg ctaaaattga cttgaacgtg atgatctatg atctttcaag
    20161 cactttttgc cgcgggtaga aagtgctttt tttattaatt ttaaaaaaag caccaaaaat
    20221 ttaaatggag gtgtctgaat gtctgctatc attgaagcta aaaaacaact agttgatgaa
    20281 attgctgagg tactatcaaa ttcagtttca acagtaatcg ttgactaccg tggattaaca
    20341 gtagctgaag ttactgactt acgttcacaa ttacgtgaag ctggtgttga gtataaagta
    20401 tacaaaaaca ctatggtacg tcgtgcagct gaaaaagctg gtatcgaagg cttagatgaa
    20461 ttcttaacag gtcctactgc tattgcaact tcaagtgaag atgctgtagc tgcagcgaaa
    20521 gtaatttctg gatttgctaa agatcatgaa gcattagaaa ttaaatcagg cgttatggaa
    20581 ggcaatgtta ttacagcaga agaagttaaa actgttggtt cattaccttc acacgatggt
    20641 cttgtatcta tgcttttatc agtattacaa gctcctgtac gcaacttcgc ttatgcggtt
    20701 aaagctattg gagaacaaaa agaagaaaac gctgaataat ttttagcgta aaaaaattaa
    20761 aaataatgga ggaattataa aatggctaat catgaacaaa tcattgaaac gattaaagaa
    20821 atgtcagtat tagaattaaa cgacttagta aaagcaattg aagaagaatt tggtgtaact
    20881 gcagctgctc cagtagcagt agcaggtgca gctggtggcg ctgacgctgc agcagaaaaa
    20941 actgaatttg acgttgagtt aacttcagct ggttcatcta aaatcaaagt tgttaaagct
    21001 gttaaagaag caactggttt aggattaaaa gatgctaaag aattagtaga cggagctcct
    21061 aaagtaatca aagaagcttt acctaaagaa gaagctgaaa aacttaaaga acaattagaa
    21121 gaagttggag ctactgtaga attaaaataa ttcaagtatc ttaaacttaa taatcaaagt
    21181 tttatagcaa gtattgctat aatataatga ttctttgaga agttaaaacc ccgttatttt
    21241 gataacgggg ttttattcat ttaaagacta agtgaaatgt tataattata atgacgagtt
    21301 acaaagtgaa gatgaggtgg aataatgagt cattattacg atgaagatcc aagtgtaatt
    21361 agcaatgaac aacgtattca atatcaatta aaccatcata aaattgattt aataactgat
    21421 aacggagtgt tttcgaaaga taaagtagat tatggttcag atgttcttgt tcaaactttt
    21481 ttaaaagcgc atccacctgg tccaagtaag cgaattgccg atgttggttg tggttacgga
    21541 ccaattggtt tgatgatcgc taaagtatca ccacatcatt caattacaat gctagatgtt
    21601 aatcacagag cgctagcctt agttgaaaaa aacaaaaaat taaatggtat tgataatgtg
    21661 atcgtaaagg aaagtgatgc tttgtctgct gtggaagaca aaagttttga ttttatttta
    21721 accaatccac caataagagc agggaaagaa accgtgcatc gtatattcga gcaagcatta
    21781 catagattag actcgaacgg tgaactattt gttgtaattc agaagaagca aggtatgcca
    21841 tctgcaaaga aaagaatgaa tgaacttttt ggaaatgtag aagtggtaaa taaagataaa
    21901 ggatattaca ttctgagaag tataaaagct tgaaatgaaa tggatattct gttatagtta
    21961 tttaatgtaa aaatttatgt tcaataagtg tgtactttta cgttaaatag ataagttaat
    22021 taagaataaa tatagaatcg aaaatggtgt catcattagt gttgccgttt tctttttgtc
    22081 tttttattaa tatgcttatg gtatttagct aaaagcggat cacataattt ttgaggggtg
    22141 aatctgtttg gcaggtcaag ttgtccaata tggaagacat cgtaaacgta gaaactacgc
    22201 gagaatttca gaagtattag aattaccaaa cttaatagaa attcaaacta aatcttacga
    22261 gtggttccta agagaaggtt taatcgaaat gtttagagac atttctccaa ttgaagattt
    22321 tactggtaat ttgtcattag agtttgtgga ttaccgttta ggagaaccaa aatatgattt
    22381 agaagaatct aaaaaccgtg acgctactta tgctgcacct cttcgtgtaa aagtgcgtct
    22441 aatcattaaa gaaacaggag aagttaaaga acaagaagtc tttatgggtg atttcccatt
    22501 aatgactgat acaggtacgt tcgttatcaa tggtgcagaa cgtgtaatcg tatctcaatt
    22561 agttcgttca ccatccgttt atttcaatga aaaaatcgac aaaaatggtc gtgaaaacta
    22621 tgatgcaaca attattccaa accgaggtgc atggttagaa tatgaaacag atgctaaaga
    22681 tgttgtatac gtgcgtattg atagaacacg taaactacca ttaacagtat tgttacgtgc
    22741 attaggtttc tcaagtgacc aagaaattgt tgacctttta ggtgacaatg aatatttacg
    22801 taatacttta gagaaagacg gcactgaaaa cactgaacaa gcgttattag aaatctatga
    22861 acgtttacgt ccaggtgaac caccaactgt tgaaaatgct aaaagtctat tgtattcacg
    22921 tttctttgat ccaaaacgct atgacttagc aagcgtgggt cgttataaaa caaacaaaaa
    22981 attacattta aaacatcgtt tattcaatca aaaattagct gagccaattg tgaatactga
    23041 aactggtgaa attgtagttg aagaaggtac agtgcttgat cgtcgtaaaa tcgacgaaat
    23101 catggatgta cttgaatcaa acgcaaacag cgaagtgttt gaattgcatg gtagcgttat
    23161 agacgagcca gtagaaattc aatcaattaa agtatatgtt cctaacgatg atgaaggtcg
    23221 tacgacaact gtaattggta atgctttccc tgactcagaa gttaaatgta ttacaccggc
    23281 agatatcatc gcttcaatga gttacttctt taacttatta agtggtattg gatatacaga
    23341 tgatattgac catttaggta accgtcgttt acgttctgta ggtgaattac tacaaaacca
    23401 attccgtatc ggtttatcaa gaatggaaag agttgtacgt gaaagaatgt caattcaaga
    23461 tactgagtct atcacacctc aacaattaat taatattcga cctgttattg catctattaa
    23521 agaattcttt ggtagctctc aattatcaca attcatggac caagcaaatc cattagctga
    23581 gttaacgcat aaacgtcgtc tatcagcatt aggacctggt ggtttaacac gtgaacgtgc
    23641 tcaaatggaa gtgcgtgacg ttcactactc tcactatggc cgtatgtgtc caattgaaac
    23701 gcctgaggga ccaaacattg gattgattaa ctcattatca agttatgcac gtgtaaatga
    23761 attcggcttt attgaaacac catatcgtaa agttgattta gatacacatg ctatcactga
    23821 tcaaattgac tatttaacag ctgacgaaga agatagctat gttgtagcac aagcaaactc
    23881 taaattagat gaaaatggtc gtttcatgga tgatgaagtt gtatgtcgtt tccgtggtaa
    23941 caatacagtt atggctaaag aaaaaatgga ttatatggat gtatcgccga agcaagttgt
    24001 ttcagcagcg acagcatgta ttccattctt agaaaatgat gactcaaacc gtgcattgat
    24061 gggtgcgaac atgcaacgtc aagcagtgcc tttgatgaat ccagaagcac catttgttgg
    24121 tacaggtatg gaacacgttg cagcacgtga ttctggtgca gctattacag ctaagcacag
    24181 aggtcgtgtt gaacatgttg aatctaatga aattcttgta cgtcgtctag ttgaagagaa
    24241 cggcgttgag catgaaggtg aattagatcg ctatccatta gctaaattta aacgttcaaa
    24301 ctcaggtaca tgttacaacc aacgtccaat cgttgcagtt ggagatgttg ttgagtttaa
    24361 cgagatttta gcagatggac catctatgga attaggagaa atggcattag gtagaaacgt
    24421 agtagttggt ttcatgactt gggacggtta caactatgag gatgccgtta tcatgagtga
    24481 aagacttgtg aaagatgacg tgtatacttc tattcatatt gaagagtatg aatcagaagc
    24541 acgtgatact aagttaggac ctgaagaaat cacaagagat attcctaatg tttctgaaag
    24601 tgcacttaag aacttagacg atcgtggtat cgtttatatt ggtgcagaag taaaagatgg
    24661 agatatttta gttggtaaag taacgcctaa aggtgtaact gagttaactg ccgaagaaag
    24721 attgttacat gcaatctttg gtgaaaaagc acgtgaagtt agagatactt cattacgtgt
    24781 acctcacggc gctggcggta tcgttcttga tgtaaaagta ttcaatcgtg aagaaggcga
    24841 cgacacatta tcacctggtg taaaccaatt agtacgtgta tatatcgttc aaaaacgtaa
    24901 aattcatgtt ggtgataaga tgtgtggtcg acatggtaac aaaggtgtca tttctaagat
    24961 tgttcctgaa gaagatatgc cttacttacc agatggacgt ccgattgata tcatgttaaa
    25021 tcctcttggt gtaccatctc gtatgaacat cggacaagta ttagagctac acttaggtat
    25081 ggctgctaaa aatcttggta ttcacgttgc atcaccagta tttgacggtg caaacgatga
    25141 cgatgtatgg tcaacaattg aagaagctgg tatggctcgt gatggtaaaa ctgtacttta
    25201 tgatggacgt acaggtgaac cattcgataa ccgtatttca gtaggtgtaa tgtacatgtt
    25261 gaaacttgcg cacatggttg atgataaatt acatgcgcgt tcaacaggac catattcact
    25321 tgttacacaa caaccacttg gcggtaaagc gcaattcggt ggacaacgtt tcggtgagat
    25381 ggaggtatgg gcacttgaag catatggtgc tgcatacaca ttacaagaaa tcttaactta
    25441 caaatccgat gatacagtag gacgtgtgaa aacatacgag gctattgtta aaggtgaaaa
    25501 catctctaga ccaagtgttc cagaatcatt ccgagtattg atgaaagaat tacaaagttt
    25561 aggtttagat gtaaaagtta tggatgagca agataatgaa atcgaaatga cagacgttga
    25621 tgacgatgat gttgtagaac gcaaagtaga tttacaacaa aatgatgctc ctgaaacaca
    25681 aaaagaagtt actgattaat acgcaattta caaaacaggc aaaaagatac taagctgaat
    25741 tttattgatg attcggttta gtactttaag ccattttaaa taaatgcaaa tcaatcaaat
    25801 agcacagcta atctaaattg aaggaggtag gctccttgat tgatgtaaat aatttccatt
    25861 atatgaaaat aggattggct tcacctgaaa aaatccgttc ttggtctttt ggtgaagtta
    25921 aaaaacctga aacaatcaac taccgtacat taaaacctga aaaagatggt ctattctgtg
    25981 aaagaatttt cggacctaca aaagactggg aatgtagttg tggtaaatac aaacgtgttc
    26041 gctacaaagg catggtctgt gacagatgtg gagttgaagt aactaaatct aaagtacgtc
    26101 gtgaaagaat gggtcacatt gaacttgctg ctccagtttc tcacatttgg tatttcaaag
    26161 gtataccaag tcgtatggga ttattacttg acatgtcacc aagagcatta gaagaagtta
    26221 tttactttgc ttcttatgtt gttgtagatc caggtccaac tggtttagaa aagaaaactt
    26281 tattatctga agctgaattc agagattatt atgataaata cccaggtcaa ttcgttgcaa
    26341 aaatgggtgc agaaggtatt aaagatttac ttgaagagat tgatcttgac gaagaactta
    26401 aattgttacg cgatgagttg gaatcagcta ctggtcaaag acttactcgt gcaattaaac
    26461 gtttagaagt tgttgaatca ttccgtaatt caggtaacaa accttcatgg atgattttag
    26521 atgtacttcc aatcatccca ccagaaattc gtccaatggt tcaattagat ggtggacgat
    26581 ttgcaacaag tgacttaaac gacttatacc gtcgtgtaat taatcgaaat aatcgtttga
    26641 aacgtttatt agatttaggt gcacctggta tcatcgttca aaacgaaaaa cgtatgttac
    26701 aagaagccgt tgacgcttta attgataatg gtcgtcgtgg tcgtccagtt actggcccag
    26761 gtaaccgtcc attaaaatct ttatctcata tgttaaaagg taaacaaggt cgtttccgtc
    26821 aaaacctact tggtaaacgt gttgactatt caggacgttc agttatcgcg gtaggtccaa
    26881 gcttgaaaat gtaccaatgt ggtttaccga aagaaatggc acttgaacta tttaaaccat
    26941 ttgtaatgaa agaattagtt caacgtgaaa ttgcaactaa cattaaaaat gcgaagagta
    27001 aaatcgaacg catggatgat gaagtttggg acgtattgga agaagtaatt agagaacatc
    27061 ctgtattact taaccgtgca ccaacacttc atagacttgg tattcaagca tttgaaccaa
    27121 ctttagttga aggtcgtgcg attcgtctac atccacttgt aacaacagct tataacgctg
    27181 actttgatgg tgaccaaatg gcggttcacg ttcctttatc aaaagaggca caagctgaag
    27241 caagaatgtt gatgttagca gcacaaaaca tcttgaaccc taaagatggt aaaccagtag
    27301 ttacaccatc acaagatatg gtacttggta actattacct tactttagaa agaaaagatg
    27361 cagtaaatac aggcgcaatc tttaataata caaatgaagt gttaaaagca tatgcaaatg
    27421 gctttgtaca tttacacaca agaattggtg tacatgcaag ttcattcaac aacccaacat
    27481 ttactgaaga acaaaacaaa aagattcttg ctacgtcagt aggtaaaatt atattcaatg
    27541 aaatcattcc ggattcattt gcttatatta atgaacctac gcaagaaaac ttagaaagaa
    27601 agacaccaaa cagatacttc atcgatccta caactttagg tgaaggtgga ttaaaagaat
    27661 actttgaaaa tgaagaatta attgaacctt tcaacaaaaa attcttaggt aatattattg
    27721 cagaagtatt caacagattt agcatcactg atacatcaat gatgttagac cgtatgaaag
    27781 acttaggatt caaattctca tctaaagctg gtattacagt aggtgttgct gatatcgtag
    27841 tattacctga taagcaacaa atacttgatg agcatgaaaa attagtcgac agaattacaa
    27901 aacaattcaa tcgtggttta atcactgaag aagaaagata taatgcagtt gttgaaattt
    27961 ggacagatgc aaaagatcaa attcaaggtg aattgatgca atcacttgat aaaactaacc
    28021 caatcttcat gatgagtgat tcaggtgccc gtggtaacgc atctaacttt acacagttag
    28081 caggtatgcg tggattgatg gccgcaccat ctggtaagat tatcgaatta ccaatcacat
    28141 cttcattccg tgaaggttta acagtacttg aatacttcat ctcaactcac ggtgcgcgta
    28201 aaggtcttgc cgatacagca cttaagacag ctgactcagg atatcttact cgtcgtcttg
    28261 ttgacgtggc acaagatgtt attgttcgtg aagaagactg tggtacagat agaggtttat
    28321 tagtttctga tattaaagaa ggtacagaaa tgattgaacc atttatcgaa cgtattgaag
    28381 gtcgttattc taaagaaaca attcgtcatc ctgaaactga tgaagttatc attcgtcctg
    28441 atgaattaat tacacctgaa attgctaaga aaattacaga tgctggtatt gaacaaatgt
    28501 atattcgctc agcatttact tgtaacgcaa gacacggtgt ttgtgaaaaa tgttacggta
    28561 aaaaccttgc tactggtgaa aaagttgaag ttggtgaagc agttggtaca attgcagccc
    28621 aatctatcgg tgaaccaggt acacagctta caatgcgtac attccataca ggtggggtag
    28681 caggtagcga tatcacacaa ggtcttcctc gtattcaaga gattttcgaa gcacgtaacc
    28741 ctaaaggtca agcggtaatt acggaaatcg aaggtgtcgt agaagatatt aaattagcaa
    28801 aagatagaca acaagaaatt gttgttaaag gtgctaatga aacaagatca tatcttgctt
    28861 caggtacttc aagaattatt gtagaaatcg gtcaaccagt acaacgtggt gaagtattaa
    28921 ctgaaggttc tattgaacct aagaattact tatctgttgc tggattaaac gcgactgaaa
    28981 gctacttatt aaaagaagta caaaaagttt accgtatgca aggggtagaa atcgacgata
    29041 aacacgttga ggttatggtt cgacaaatgt tacgtaaagt tagaattatc gaagcaggtg
    29101 atacgaagtt attaccaggt tcattagttg atattcacaa ctttacagat gcaaatagag
    29161 aagcatttaa acaccgcaag cgccctgcaa cagctaaacc agtattactt ggtattacta
    29221 aagcatcact tgaaacagaa agtttcttat ctgcagcatc attccaagaa acaacaagag
    29281 ttctaacaga tgcagcaatt aaaggtaagc gtgatgactt attaggtctt aaagaaaacg
    29341 taattatcgg taagctaatt ccagctggta ctggtatgag acgttatagc gacgtaaaat
    29401 acgaaaaaac agctaaacca gttgcagaag ttgaatctca aactgaagta acggaataac
    29461 aagtatataa cagaggctaa ggctttagcc tcttgttatt tttatgtaaa ttatttgatt
    29521 taatgttgac gaattctctt gttcaatgtt aatatattaa aggttgatgc aagcagaact
    29581 ttggaggata aattattgtc taaggaaaaa gttgcacgct ttaacaaaca acattttgta
    29641 gttggtctta aagaaacgct taaagcgtta aagaaagatc aagttacatc tttgattatt
    29701 gctgaagacg ttgaagtata tttaatgact cgcgtgttaa gccaaatcaa tcagaaaaat
    29761 atacctgtat cttttttcaa aagcaaacat gctttgggta aacatgtagg tattaacgtc
    29821 aatgcgacaa tagtagcatt gattaaatga gaattagtaa gtgtttaact tactaaattt
    29881 tatttaacct aaaaatgaac cacctggatg tgtgggatta aaaagtgaag agaggaggac
    29941 atatcacatg ccaactatta accaattagt acgtaaacca agacaaagca aaatcaaaaa
    30001 atcagattct ccagctttaa ataaaggttt caacagtaaa aagaaaaaat ttactgactt
    30061 aaactcacca caaaaacgtg gtgtatgtac tcgtgtaggt acaatgacac ctaaaaaacc
    30121 taactcagcg ttacgtaaat atgcacgtgt gcgtttatca aacaacatcg aaattaacgc
    30181 atacatccct ggtatcggac ataacttaca agaacacagt gttgtacttg tacgtggtgg
    30241 acgtgtaaaa gacttaccag gtgtgcgtta ccatattgta cgtggagcac ttgatacttc
    30301 aggtgttgac ggacgtagac aaggtcgttc attatacgga actaagaaac ctaaaaacta
    30361 agaatttagt ttttaattaa atcttaaact taaaatattt aatataagga agggaggatt
    30421 tacattatgc ctcgtaaagg atcagtacct aaaagagacg tattaccaga tccaattcat
    30481 aactctaagt tagtaactaa attaattaac aaaattatgt tagatggtaa acgtggaaca
    30541 gcacaaagaa ttctttattc agcattcgac ctagttgaac aacgcagtgg tcgtgatgca
    30601 ttagaagtat tcgaagaagc aatcaacaac attatgccag tattagaagt taaagctcgt
    30661 cgtgtaggtg gttctaacta tcaagtacca gtagaagttc gtccagagcg tcgtactact
    30721 ttaggtttac gttggttagt taactatgca cgtcttcgtg gtgaaaaaac gatggaagat
    30781 cgtttagcta acgaaatttt agatgcagca aataatacag gtggtgccgt taagaaacgt
    30841 gaggacactc acaaaatggc tgaagcaaac aaagcatttg ctcactaccg ttggtaagat
    30901 aaaagctttt accctgagtg tgttctatat taatgaattt tcattaagcg ttcatgctta
    30961 gggcatcgcc atatctatcg tatttattca gtaatataaa ctggaaggag aaaaaataca
    31021 tggctagaga attttcatta gaaaaaactc gtaatatcgg tatcatggct cacattgatg
    31081 ctggtaaaac gactacgact gaacgtattc tttattacac tggccgtatc cacaaaattg
    31141 gtgaaacaca tgaaggtgct tcacaaatgg actggatgga gcaagaacaa gaccgtggta
    31201 ttactatcac atctgctgca acaacagcag cttgggaagg tcaccgtgta aacattatcg
    31261 atacacctgg acacgtagac ttcactgtag aagttgaacg ttcattacgt gtacttgacg
    31321 gagcagttac agtacttgat gcacagtcag gtgttgaacc tcaaactgaa acagtttggc
    31381 gtcaggctac aacttatggt gttccacgta tcgtatttgt aaacaaaatg gacaaattag
    31441 gtgctaactt cgaatactct gtaagtacat tacatgatcg tttacaagct aacgctgctc
    31501 caatccaatt accaattggt gcggaagacg aattcgaagc aatcattgac ttagttgaaa
    31561 tgaaatgttt caaatataca aatgatttag gtactgaaat tgaagaaatt gaaattcctg
    31621 aagaccactt agatagagct gaagaagctc gtgctagctt aatcgaagca gttgcagaaa
    31681 ctagcgacga attaatggaa aaatatcttg gtgacgaaga aatttcagtt tctgaattaa
    31741 aagaagctat ccgccaagct actactaacg tagaattcta cccagtactt tgtggtacag
    31801 ctttcaaaaa caaaggtgtt caattaatgc ttgacgctgt aattgattac ttaccttcac
    31861 cactagacgt taaaccaatt attggtcacc gtgctagcaa ccctgaagaa gaagtaatcg
    31921 cgaaagcaga cgattcagct gaattcgctg cattagcgtt caaagttatg actgaccctt
    31981 atgttggtaa attaacattc ttccgtgtgt actcaggtac aatgacatct ggttcatacg
    32041 ttaagaactc tactaaaggt aaacgtgaac gtgtaggtcg tttattacaa atgcacgcta
    32101 actcacgtca agaaatcgat actgtatact ctggagatat cgctgctgcg gtaggtctta
    32161 aagatacagg tactggtgat actttatgtg gtgagaaaaa tgacattatc ttggaatcaa
    32221 tggaattccc agagccagtt attcacttat cagtagagcc aaaatctaaa gctgaccaag
    32281 ataaaatgac tcaagcttta gttaaattac aagaagaaga cccaacattc catgcacaca
    32341 ctgacgaaga aactggacaa gttatcatcg gtggtatggg tgagcttcac ttagacatct
    32401 tagtagaccg tatgaagaaa gaattcaacg ttgaatgtaa cgtaggtgct ccaatggttt
    32461 catatcgtga aacattcaaa tcatctgcac aagttcaagg taaattctct cgtcaatctg
    32521 gtggtcgtgg tcaatacggt gatgttcaca ttgaattcac accaaacgaa acaggcgcag
    32581 gtttcgaatt cgaaaacgct atcgttggtg gtgtagttcc tcgtgaatac attccatcag
    32641 tagaagctgg tcttaaagat gctatggaaa atggtgtctt agcaggttat ccattaattg
    32701 atgttaaagc taaattatat gatggttcat accatgatgt cgattcatct gaaatggcct
    32761 tcaaagttgc tgcatcatta gcacttaaag aagctgctaa aaaatgtgat cctgtaatct
    32821 tagaaccaat gatgaaagta actattgaaa tgcctgaaga gtacatgggt gatatcatgg
    32881 gtgacgtaac atctcgtcgt ggacgtgttg atggtatgga acctcgtggt aatgcacaag
    32941 ttgttaatgc ttatgtacca ctttcagaaa tgttcggtta tgcaacatca ttacgttcaa
    33001 acactcaagg tcgcggtact tacactatgt acttcgatca ctatgctgaa gttccaaaat
    33061 caatcgctga agatattatc aagaaaaata aaggtgaata atataacttg ttttgactag
    33121 ctagcctagg ttaaaataca aggtgagctt aaatgtaagc tatcatcttt atagtttgat
    33181 tttttggggt gaatgcatta taaaagaatt gtaaaattct ttttgcatcg ctataaataa
    33241 tttctcatga tggtgagaaa ctatcatgag agataaattt aaatattatt tttaattaga
    33301 ataggagaga ttttataatg gcaaaagaaa aatttgatcg ttctaaagaa catgccaata
    33361 tcggtactat cggtcacgtt gaccatggta aaacaacatt aacagcagca atcgctactg
    33421 tattagcaaa aaatggtgac tcagttgcac aatcatacga catgattgac aacgctccag
    33481 aagaaaaaga acgtggtatc acaatcaata cttcacacat tgagtaccaa actgacaaac
    33541 gtcactacgc tcacgttgac tgcccaggac acgctgacta tgttaaaaac atgatcactg
    33601 gtgctgctca aatggacggt ggtatcttag tagtatctgc tgctgacggt ccaatgccac
    33661 aaactcgtga acacattctt ttatcacgta acgttggtgt accagcatta gtagtattct
    33721 taaacaaagt tgacatggtt gacgatgaag aattattaga attagtagaa atggaagttc
    33781 gtgacttatt aagcgaatat gacttcccag gtgacgatgt acctgtaatc gctggttcag
    33841 cattaaaagc tttagaaggc gatgctcaat acgaagaaaa aatcttagaa ttaatggaag
    33901 ctgtagatac ttacattcca actccagaac gtgattctga caaaccattc atgatgccag
    33961 ttgaggacgt attctcaatc actggtcgtg gtactgttgc tacaggccgt gttgaacgtg
    34021 gtcaaatcaa agttggtgaa gaagttgaaa tcatcggttt acatgacaca tctaaaacaa
    34081 ctgttacagg tgttgaaatg ttccgtaaat tattagacta cgctgaagct ggtgacaaca
    34141 ttggtgcatt attacgtggt gttgctcgtg aagacgtaca acgtggtcaa gtattagctg
    34201 ctcctggttc aattacacca catactgaat tcaaagcaga agtatacgta ttatcaaaag
    34261 acgaaggtgg acgtcacact ccattcttct caaactatcg tccacaattc tatttccgta
    34321 ctactgacgt aactggtgtt gttcacttac cagaaggtac tgaaatggta atgcctggtg
    34381 ataacgttga aatgacagta gaattaatcg ctccaatcgc gattgaagac ggtactcgtt
    34441 tctcaatccg tgaaggtgga cgtactgtag gatcaggcgt tgttactgaa atcattaaat
    34501 aatttctaat ttcttagatt ttatataaaa agaagatccc tcaatcgagg ggtctttttt
    34561 ttaatgtgta aattttgtaa tggctgttca atttagaaga acaataattg atgagaggtt
    34621 gactaataaa acttataact gataatacag tttaaataaa attgttgagc cttggacatt
    34681 gtaaaatgct cctctcaaag ttttcatttt tcaatgtcta ctttgaagga gcattacatt
    34741 agtttatgtc tcagattcat atctttcaat taatttaaat gcttaatttg ttttaaatac
    34801 ttgctctaat tcaatgattt tcaaaaatac agctacagcg tattttaatg atttttcatc
    34861 aatatcaaat ttgggattat ggtgtggcgc tgtaatac
//
LOCUS       DAMZPP010000009        32554 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMZPP010000009 DAMZPP010000000
VERSION     DAMZPP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.009"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(484..1038)
                     /locus_tag="POH73_000334"
     CDS             complement(484..1038)
                     /locus_tag="POH73_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDI6886318.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAN
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            complement(1358..2758)
                     /locus_tag="POH73_000335"
     CDS             complement(1358..2758)
                     /locus_tag="POH73_000335"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HDI6886319.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPFGKLRAGFDLFKKPTQMQNGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYSNQQEDVF
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTYDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDDELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            complement(2782..3705)
                     /gene="hemH"
                     /locus_tag="POH73_000336"
     CDS             complement(2782..3705)
                     /gene="hemH"
                     /locus_tag="POH73_000336"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDI6886320.1"
                     /translation="MTKKMGLLVVAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHKD
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            complement(3763..4800)
                     /gene="hemE"
                     /locus_tag="POH73_000337"
     CDS             complement(3763..4800)
                     /gene="hemE"
                     /locus_tag="POH73_000337"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDI6886321.1"
                     /translation="MVHNKNNTILKMIKGEETTHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQRL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSGFVHTYTQR"
     gene            complement(4790..4897)
                     /locus_tag="POH73_000338"
                     /pseudo
     CDS             complement(4790..4897)
                     /locus_tag="POH73_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5062..5565
                     /gene="traP"
                     /locus_tag="POH73_000339"
     CDS             5062..5565
                     /gene="traP"
                     /locus_tag="POH73_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HDI6886322.1"
                     /translation="MKKLYTSYGTYGFLNQIKINNPSHHLFQFSTADSSVIFEETEEN
                     TVLKSPSIYEVIKEIGAFNEDHFYCAIFIPSTEDHVYQLEKKLISVDDNFKNFGGFKS
                     YRLLRPVKGTTYKIYFGFADRQTYEDFKNSDAFKDHFSKEALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            complement(5689..6912)
                     /locus_tag="POH73_000340"
     CDS             complement(5689..6912)
                     /locus_tag="POH73_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6886323.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFIIFALTYYLLLAPKWYITFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGNKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLIVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEDKVIKGYEQFLYRLMFVICTVFALTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(6905..7645)
                     /locus_tag="POH73_000341"
     CDS             complement(6905..7645)
                     /locus_tag="POH73_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6886324.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDEAMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            7779..8201
                     /locus_tag="POH73_000342"
     CDS             7779..8201
                     /locus_tag="POH73_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDI6886325.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            8343..8708
                     /locus_tag="POH73_000343"
     CDS             8343..8708
                     /locus_tag="POH73_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDI6886326.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            9441..9998
                     /locus_tag="POH73_000344"
     CDS             9441..9998
                     /locus_tag="POH73_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDI6886327.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNFGISITDLLYLKIIIFS
                     NYGQYIEEHSTGINILKKIKNPYHL"
     gene            10203..11165
                     /locus_tag="POH73_000345"
     CDS             10203..11165
                     /locus_tag="POH73_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HDI6886328.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFKKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSDVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
     gene            complement(11286..12227)
                     /gene="yhaM"
                     /locus_tag="POH73_000346"
     CDS             complement(11286..12227)
                     /gene="yhaM"
                     /locus_tag="POH73_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HDI6886329.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(12224..15160)
                     /locus_tag="POH73_000347"
     CDS             complement(12224..15160)
                     /locus_tag="POH73_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6886330.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQANIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTVFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVYYSEAITDEIEVIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            complement(15150..16346)
                     /locus_tag="POH73_000348"
     CDS             complement(15150..16346)
                     /locus_tag="POH73_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDI6886331.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            complement(17234..17578)
                     /locus_tag="POH73_000349"
     CDS             complement(17234..17578)
                     /locus_tag="POH73_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HDI6886332.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            complement(17647..18771)
                     /locus_tag="POH73_000350"
     CDS             complement(17647..18771)
                     /locus_tag="POH73_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDI6886333.1"
                     /translation="MNALFIIIFMIVVGAIIGGVTNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIASQLNNYYTKHQNQTIASLLPNQLVTFLDQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARAIVTSLITNEYQTFKDKSLNKLLDTSQFNEIAENLSMYVTTYASKQ
                     ANKPVVTLMPQFVDYLESQLSSKLANLIIEQLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            complement(18950..19414)
                     /locus_tag="POH73_000351"
     CDS             complement(18950..19414)
                     /locus_tag="POH73_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6886334.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            complement(19772..20395)
                     /locus_tag="POH73_000352"
     CDS             complement(19772..20395)
                     /locus_tag="POH73_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6886335.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            complement(20417..21529)
                     /locus_tag="POH73_000353"
     CDS             complement(20417..21529)
                     /locus_tag="POH73_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HDI6886336.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            21692..22513
                     /locus_tag="POH73_000354"
     CDS             21692..22513
                     /locus_tag="POH73_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDI6886337.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTVDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKETVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            complement(22969..24354)
                     /gene="fumC"
                     /locus_tag="POH73_000355"
     CDS             complement(22969..24354)
                     /gene="fumC"
                     /locus_tag="POH73_000355"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDI6886338.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAALANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHHSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAQYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(24550..24945)
                     /locus_tag="POH73_000356"
     CDS             complement(24550..24945)
                     /locus_tag="POH73_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886339.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPIIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITGILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            complement(25546..25698)
                     /locus_tag="POH73_000357"
     CDS             complement(25546..25698)
                     /locus_tag="POH73_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886340.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
     gene            complement(25723..26322)
                     /locus_tag="POH73_000358"
     CDS             complement(25723..26322)
                     /locus_tag="POH73_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HDI6886341.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNIEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            complement(26481..26951)
                     /gene="trmL"
                     /locus_tag="POH73_000359"
     CDS             complement(26481..26951)
                     /gene="trmL"
                     /locus_tag="POH73_000359"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDI6886342.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEATNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            complement(26956..28083)
                     /gene="queG"
                     /locus_tag="POH73_000360"
     CDS             complement(26956..28083)
                     /gene="queG"
                     /locus_tag="POH73_000360"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDI6886343.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPMIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            complement(28234..28956)
                     /locus_tag="POH73_000361"
     CDS             complement(28234..28956)
                     /locus_tag="POH73_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDI6886344.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            complement(28949..30406)
                     /locus_tag="POH73_000362"
     CDS             complement(28949..30406)
                     /locus_tag="POH73_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDI6886345.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPAAYAEQDQKWEEIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNDYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGVVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLLVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            complement(30665..31726)
                     /locus_tag="POH73_000363"
     CDS             complement(30665..31726)
                     /locus_tag="POH73_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDI6886346.1"
                     /translation="MSNAKNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
     gene            complement(32273..32356)
                     /locus_tag="POH73_000364"
     tRNA            complement(32273..32356)
                     /locus_tag="POH73_000364"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32320..32322),aa:Leu,seq:caa)
     gene            complement(32395..32469)
                     /locus_tag="POH73_000365"
     tRNA            complement(32395..32469)
                     /locus_tag="POH73_000365"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32434..32436),aa:Gly,seq:tcc)
     gene            complement(32477..32550)
                     /locus_tag="POH73_000366"
     tRNA            complement(32477..32550)
                     /locus_tag="POH73_000366"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32516..32518),aa:Cys,seq:gca)
BASE COUNT        10513 a         5773 c         4544 g        11724 t
ORIGIN      
        1 ctccatcccg cgctaatatt attttgaatt acctaattaa tataccataa tcaaaaacct
       61 aaagtcaaga actttttgaa tttaatttaa atgttatctc ttaaataatt acttaaatat
      121 cgtagcaaca tgttctctgt tgaacacaaa tattagtata ttcatttttg tagtatacgt
      181 caacgacatt tttaaagttt tttgtgtaaa aaaacgcttc ttattccctt ttatcatata
      241 agtgtctaat agttgttata aatagtgtta cagcatttta aaaggtatag gaattataaa
      301 gtttacaacg cctatacctt ctgaaaaaga aatttaaaag cttgctacaa ccgcatgctc
      361 attcagtcaa cgactaccaa tataacattg tagccctaag acattccttg gcgcttcaat
      421 taccacattt ttttcaaaat cagcagctac ctacagacac aacataacac aacaatacat
      481 tacctatcgt gtcatgtaat cttgcatccg atcttgcaac gctgtaaatg tttcgaagcc
      541 atcctcttct aagaagtgcc ctccatcttc cacgattcgc aagttcccct ctaatgcatt
      601 cattaaacgc tgagtctctt tatatgaaac gtatttgtca tttttagaac tcaatccgta
      661 aaaattgtca actttctttt taatattatc gtaatcaata gttacattac ttaaatcgat
      721 atctaaatct atattttctg catcttcttt aaagcccgct atactgaaaa agccttcaat
      781 cggctgatca atcatttcaa tatattttaa agctgtgatt gaacctaaac catgtgttac
      841 aaaatatgta tcctttttgc gtacattaat ttgtttcgtc atagcttcaa tccactgatc
      901 cactgtattc gcttcagggg attcaaaatt aaataatgtt acgtcatatc cttctaaagt
      961 taagttatgc tccaaccact gataccaatg atttctacta tttccatgca tagaatgtac
     1021 aatgattaca tctgtcatct cattctctcc tttcaactta ctacttcttt tctatttaaa
     1081 aaaatgaatg attacctata attgtaaaat aaaaacacct taattagaaa tgttatatcg
     1141 caaagtgaca tttctaatta aggtgcattg tcatcatttc aatatcattc aataatagct
     1201 aaaccttctt cttcgattca gtttcatgaa ataattcccg ctgaaagtat catgatttac
     1261 acattacttc caccattata taacttaaaa atgactatat ttcatcaaac attatctaaa
     1321 ggcgtcgcac ctacaccaac tccatccaac aattaactta caactctgcg attacttctt
     1381 cagcagcaac tttaccttgt gtaatacaat caggtagtcc aaccgcttca aaagatgcac
     1441 cagttactct aagtcgtgga tatgtttgtt taatatgtgc ttgaatctgc ctaatttgtt
     1501 gaatgtgacc aacatggtac tgtggcatac ttttcggcaa acgattgaca attgtaaatt
     1561 caggatcgcc tttaaatgtc atcatttgac ttaaatctct acgtacaatc gatactaatt
     1621 catcatctgt atgatcatca accacagtat cacctggttt acctacatac gcacgaatca
     1681 aaaccttacc ttctggtgta gtaaatggcc attttttcga tgtccaagta catgcggtaa
     1741 tgtcagtatc actcgttctc gcaatcacga agccagtacc atcataagta ttttcaatgt
     1801 ctttttcatc aaatgccaat acaacagttg caacagtcgt actatccatc gttttaaaat
     1861 aatcaaatgc tggatcttgt ccgaaccaat tcaaaaaaac ttgatgcggt gttgtcacta
     1921 ataccccatc gaatacatct tcttgttgat tactgtaaac aattttatat tgcttttgag
     1981 atgtaataat atcatccact gacgtattgt aacgtattgt cacaccttta tttttcacat
     2041 cttgttctaa tgcttcaata aatgaactta aaccatgctt aaattgtttg aattgtcctt
     2101 ttggtgcgcc aggatataat tgtctttgtt tcagacgctt atttttctca tccttcatac
     2161 cttttatcag acttccgaat gcctcttctt tttctttaaa attaggaaac gtactcatca
     2221 aacttaattt atcaatatcg gtaccataaa taccacccat taaaggctca attaaattct
     2281 caagtacctc attacctaat cttgctctga aaaatgcacc aacagaaatg tcaccattct
     2341 gcatttgagt aggtttttta aataaatcaa accctgctct taactttcca aatggtgaaa
     2401 ttaatttagt tgtcacaaac ggtttgatat ctgtcggaat tcccataatt gatccacctg
     2461 gaataggata caatttgttt ttcgcaaaaa tataagattg tccagtcgta tttgtaacaa
     2521 tatcttgttc taatccaata tctttcgcta attctgtcat aatcgttttt ctacctaaat
     2581 aagattcagg ccctagttca atcatataac catctttacg atacgattga atcttccccc
     2641 ccggacgatt cgatgcttca aagatggtta catcaatatt aggatcttgc tgttttaaaa
     2701 aatatgcact tgataaacct gttatccccg ctcctataat agccactgat ttagtcacaa
     2761 cgtttcacgc ttctttcgtc attaaaatat agacttgatt tcatcaacaa ttgcaccgat
     2821 aaataatgga tgtgtattcg gcatttttgg acgataataa ttcgcgccaa tgtcatcgca
     2881 aacaacttta cattcataat cattgtcata aagcacctct aaatgctcac atacaaaacc
     2941 tactggcgta tatataaagt ttttatactg atgtttttca tataaatcac gtgttaaatc
     3001 ttgtacatct ggccctaacc aaggtgtacc tgtattacct tcagattgcc aaccaatcgc
     3061 gatatgttca atattagatt gttctttaat taaaagcgca gtatgttcta gttcttgtgg
     3121 atatggatca ttattctttt cgattaaacc ttttggcaaa ctatgtgccg aaacaactaa
     3181 taccgtgtct ttgtgttcct cttccggtat ttgagctaat gtttcgttga ctttattcgt
     3241 ccaatattca ataaatttag gttgttcata ataatgtttc acatgtgtaa gttgaatacc
     3301 atattttgca gcttcttcat cagcacgttt gtcatatgat cctactgaaa atgaagaata
     3361 atgtggtgct agtactacag tgattgcttc agtaatacca tctttgtgca tttgttcaac
     3421 cgcatcttcg ataaatggtg aaatgtgttt taatcctaag tatagtttaa attcaacatc
     3481 tgcatatgct ttatttaatg ctgaaactag cgcatcagcc tggtcatctg ttgtacctgc
     3541 taatggtgat aaaccaccta taaattcata tctatctttc aaatcttgaa gttcttcttc
     3601 agatggacgt ttaccatgtc taatgtctgt ataatatggc tctatgtcac tttctttata
     3661 aggtgtgcca taagccacaa ctaataatcc cattttttta gtcattgata ataccttcct
     3721 ttaaatgaat tatctttcat gtgcttcaat gtaatactat gattatcttt gtgtatatgt
     3781 gtgtacgaat ccgcttactt tacgtaacgt ctctggttgc acttctggga aaacaccgtg
     3841 tcctaaatta aagatgtgtt taccgttctc cataccttga tctaatattg gtttcaatct
     3901 ctcttcaatg acattccatg gtgctaataa aattgatgga tctaaattac cttgtaatgt
     3961 tttagtaacg cctaatcgtt gagcctgatt aatagacgtt ctccaatcta ggcctaatac
     4021 atcaatcggc aaatcattcc attcattgat taaatgactg gcacctacac cgaatagaat
     4081 taccggcaca tcatgttttt ctttaacctc actgattaat cgaatcatat gtggtttaat
     4141 gtaacgtctg taatcctcga catttaatgc acctacccat gaatcgaaaa tttgaatcaa
     4201 ttcggcacct gcttcgactt gagctgttac atatttaaca gatacatcga ctaaatgatt
     4261 cattaaagca aaccatgttg cttcatctct atacatcatc gcttttgtaa aattgtaatt
     4321 tttcgatggt ccgccttcaa tcatatatga cgctaatgta aatggtgccc cagtaaatcc
     4381 tattagcggc acatttaact tttcttctgt taaaagttta attgtatcta atacatatgg
     4441 tacatctcgt tcggggtcta tttgagaaag tttctcaaca tcttgaattg ttttgatagg
     4501 attatgaatc actggaccaa tacccgattt aatttctaca tcgacaccaa ttggctttaa
     4561 tggtgtcata atatctttgt ataaaattgc tgcatctgta tgataattat caactggtaa
     4621 atgtgtaaca taagcgcaca actccggctg atgtgtaata tcgaatagtg aatatttttc
     4681 tttcaatttt cgatattctg gttgcgaacg gccagcttgt cgcataaacc aaacaggtgt
     4741 atgtgtcgtt tcttcacctt taatcatttt caaaattgta ttgtttttat tatgcaccat
     4801 aaaggcctcc taaattaaaa tcattcttat ctatattatc atatcgctca ttcgttcgta
     4861 ttttcaataa ataaatgtca taaaactgac attaaattac acatcatttg tttgtgtatt
     4921 taaattttaa aatcttgatg gattatctcc acattttttt gtcttgcatg atttattata
     4981 gtaactttaa ccgtatatta atagtggtaa tgactagttt atcgtcgtat aataaataaa
     5041 aacataaggg ggacctttca tatgaagaaa ttatatacat cttatggcac atatggattt
     5101 ttaaatcaga taaaaatcaa taatccatct catcatttat tccaattttc aactgctgat
     5161 tcatcggtga tttttgaaga gactgaagaa aatactgtac tgaaatcacc ttcaatatat
     5221 gaagttatta aagaaatagg cgcattcaat gaagatcatt tttattgcgc aatttttatt
     5281 ccgtcaactg aagaccatgt ttatcaactt gaaaagaaat taatcagtgt tgatgacaat
     5341 ttcaaaaact ttggtggctt taaaagttat cgattgctaa gacctgttaa aggtacaaca
     5401 tacaaaattt actttggctt tgctgatcga caaacttatg aagactttaa aaattctgat
     5461 gcttttaaag atcatttttc aaaagaagca ttaagtcatt actttggttc aagtggacaa
     5521 cattcaagtt attttgaaag atatttatac ccaataaaag aatagattcg aagaaaactt
     5581 ccattggtta tgctgttaac gtatagatgc taatgtaatc tgaacgccgt atcatttggc
     5641 tttcaacatt acatgcacct ttacaacata catgccaatg gaagtttttt agtctcgtaa
     5701 taatgtttcc tgatatttta actttttaat aatacttcta atcgttaata aacctacaat
     5761 atagaaaatg agtacaacat agaataacgt catatgtttt ataataaatg tcaatgcaaa
     5821 cacagtacaa ataacaaaca ttaatctata taaaaactgt tcataccctt tgattacctt
     5881 atcttcaggt acaggccaca cttgaggcca taagccatat gcttgttgtg aataaaattg
     5941 tgccatttgt aacaatataa tatatacaaa taaacaccca ataattgctg tcactaatgg
     6001 atatgataac caaactataa gtaaaactgc aataattact aacctaaaga taatattaaa
     6061 tgcgtctctc cctcttataa agcttctaat aaataagaat aaatacatcg cattagagtt
     6121 aaatttatta ccctttggaa ctggtaaaag tatatctaga taacttcttc tgactgcaga
     6181 ttctttcaaa tgttttacat cggtaaacat attaacaaat ttataataat tcatatgatg
     6241 tcgatgttcg atagcaatca ttttctccca tggatacaaa aagccaggtt tatatttttt
     6301 aactaaaaat tctattaaca caggcaaagc aaccatcaca aatgtgatgt accattttgg
     6361 agctaatagt aagtaatatg ttagagcaaa gatgatgaat gatattaaat taacttgcca
     6421 tgttttaagt cccgattgat accattgcca tcttaagcgt aaaccaacat atggaaaaat
     6481 taatgcactg actccaaaac aaatataaaa tgccacatta tgttgattaa tattgtaaaa
     6541 caacgggaac attacaataa caataatgag ttggattaaa atgcgcgcaa agtagctata
     6601 taaaatcgca tgacgcataa attgagacat gtgtttttca aatggtaata aaaagatttt
     6661 atccgcttct tttaggagtg gtcgcattgg aaaaatggat gtcaacgcaa caatcactgc
     6721 tgctattaat gaaaaattga tattcgttgg aatatgtttt aaccattcac catatccaaa
     6781 aataaatgca cccagcaaaa taagtaaaaa gaccatgaaa tgaccattaa atataaactt
     6841 attataataa tttttctctt tacgaagggc atgtaatctt ttattaaata atgtggtcgc
     6901 ttggttacgc atgtacatct ccaccttgcg tcacatgaat atatatatcg tctaacgttt
     6961 gattatgtaa gccagtttgt tgtctcaatg cttctaaatc tccaaatgca acgacttcac
     7021 cttcgtctag tatgataaaa cgatcacaat aacgttcagc tgttgctaaa atatgtgtac
     7081 tcattagaac ggttctacct tcgtttttct tttcaaccat taaatctaac atggattgaa
     7141 ttcctaatgg atctaggcca aggaatggtt cgtctataat atacaattcg ggattaacga
     7201 taaacgcaca aataatcatg actttttgtt tcatcccctt agaaaaatga ctcggaaaaa
     7261 ctttcaattc attttctaaa cggaatgtct ttaataatgg cattgctcga ttcatcgctt
     7321 catcacgatc aatatcatat gccattgctg tcatctcaat gtgttcctct aatgtgagtt
     7381 cttcgtatat aaccggtgat tccggaatat aagataactt tcttctataa gcctctatgt
     7441 catcattaat gttgatatct gaaattgata gagatccttc cataggtgta agcaatccta
     7501 gcatatgttt aatcgttgta ctcttaccag cgccattaag gccaataagt ccaacgattt
     7561 cgcctttgtt taattcaaaa tttatatctt taattacagg gcgttttcca tatccacctg
     7621 taagctgttc tactttaact gtcataaggc acctccatga cttatattgt accaaaaatt
     7681 ataaaatgct catattaaat acacatatcc taatatcgaa tttttagcga caatgttata
     7741 atgaatggta atacaagttg aaaaggagtg tagtcatcat gtcagaaaca attttcggca
     7801 aaattttaac tggagaaatt cctagcttta aagtatatga agacgattat gtctatgcct
     7861 ttttagatat atcacaagtt actaaaggac atacgttatt aattcctaaa aaagcttctg
     7921 ctaatatctt tgaaactgat gaagaaacaa tgaaacatat cggcgcagca ttacctaaag
     7981 tagcaaatgc tattaagcgt gcatttaatc ctgatggttt aaacattatt caaaataatg
     8041 gtgagtttgc agatcaatcg gtatttcata ttcatttcca cttaattcct cgatatgaaa
     8101 atgatattga tggatttggt tataagtggg aaacacatga agatatttta gataacgatg
     8161 caaaacaaca aattgctgaa caaattcaag cacaatttta aatgtatgct taatctaagc
     8221 tcgaacgggt ataatatgat taatattata acaattgcgt ttgaagtgat aacatcaagg
     8281 ttagcaattt taaacaaaat gagttattaa gataacagat gttaaaagtg aggagaatat
     8341 aaatgaaagc atcacgcatt ctattcggta tcggtgttgg cgtagcagct ggttttgtag
     8401 ttgcacttca aggacgtgac gacaaaagtg tcaagaacaa cacgattgat cgtactgccc
     8461 ctactggttc aaaatcagaa ctacaacgtg aatttgaaac gattaaacaa agttttaatg
     8521 acattttaaa ctatggtgtt caaattaaaa acgaaagtgc ggaatttggt agttcaatcg
     8581 gtggtgaaat taagtcatta cttggaaact tcaaatctga catcaatcct aatattgaac
     8641 gtttacagtc acacatcgaa aatttacaaa atcgtggcga ggatattgga aacgaaattt
     8701 ctaagtagca cgtttacgtt ctcgatcaca actattttta ttagtaacag catatttatt
     8761 ttttaaaatt aaatgccaaa taaacgagat gacattagaa attagatatt tcttgtcatc
     8821 tcttttttat ggctcgaatg aatgtttgat taccaattac agtaagacgt tgtttgtagt
     8881 gattttccct taaatcatat ttatgaattg attgaaaata ttgcttagga ttcattgtgt
     8941 tatccttgca ctttgattac gctttactta aatcattatc gacaaacaac atacttatat
     9001 tttcactgag ccgaacctta tatgcacatt acatatacct tacttccaca aattattaat
     9061 ctggtattta ttataattac atatcactat atttttagca tttgtatgac ttagttagtt
     9121 aaaagatgct tttgcatatg ccctgccata tccatttttt atattattca agttttcaaa
     9181 tactcatgtc acacgcgcct ttttcataag tgatgcttta ttagcaaaaa tatgtgttcg
     9241 cagaaatttg ttctgcattc tacttctacg ctagtcaatc agccaatttt accaatccca
     9301 ctttcgcgtt tcaaatcaaa caatacgtcg cccctttctt catatataac aaatcttcta
     9361 acatgatatg ttactattga attactgaac ctgagttagt tataatctaa cttatattga
     9421 aaagagatga ggcgtaagat atgtttttat gtaaaagaca aattgatatc aatgcacgat
     9481 ttggtttgcc tagaattgca tttatgagtg cagttgcaac catcattatg tttttagtta
     9541 gttatgaagt aatgtatttt ttatctaata cgccgttatc agatagacat tttctcatct
     9601 ttttattact tgtatttatg acgtatccat tacataaaag tatacattta ttatttttct
     9661 taccatatag aaaatcgttt aaagttcata agttgactaa aagaaaatgg cttatattct
     9721 ataataccta cgtcaatcaa cctgtacaca aattttattt ttgcattaac ttaatattgc
     9781 cgttaattat cttatctgca atgttcgttt atctaacaat ttcattcccg caatatggac
     9841 attattttat gttcttattg gcattgaatt tcggtatttc cattacagat ttattatatt
     9901 taaaaataat tatattttct aattatggac aatatataga agaacatagt acaggtatta
     9961 atattttgaa aaaaattaaa aatccatatc atttataaca aaataattat agcaaggtgt
    10021 tattatttgt tttcaggcta tgtaatagct tacaatcaaa tgtatataga ccttgttttt
    10081 ttattttcat caatttctac ccctaaacct aatgctctag tctgatgtca tgggttattg
    10141 attggtgata atataaaact atgttatatt cacgatgatt aacttacaaa ggagtttcaa
    10201 ctatgaagat gataaacaaa ttaatcgttc cggtaacagc tagtgcttta ttattaggcg
    10261 cttgtggcgc tagtgccaca gattctaaag aaaatacatt aatttcttct aaagctggag
    10321 atgtaacagt tgcagataca atgaaaaaaa tcggtaaaga tcaaattgca aatgcatcgt
    10381 ttactgaaat gttaaataaa attttagctg ataaatataa aaataaagtt aatgataaga
    10441 agattgacga acaaattgaa aaaatgcaaa agcaatacgg cggtaaagat aaatttaaaa
    10501 aggcccttca acagcaaggt ttaacagccg ataaatataa agaaaattta cgtactgctg
    10561 cttatcataa agaattacta tcagataaaa ttaaaatctc tgattctgaa attaaagaag
    10621 acagcaagaa agcttcacac attttaatta aagttaaatc taagaaaagc gacaaagaag
    10681 gcttagatga taaagaagcg aaacaaaaag ctgaagaaat tcaaaaagaa gtttcaaaag
    10741 atccaagtaa atttggtgaa atcgctaaaa aagaatcaat ggatactggt tcagctaaaa
    10801 aagatggcga attaggttat gttcttaaag gacaaactga taaagatttt gaaaaagcac
    10861 tatttaaact taaagatggt gaagtatcag acgttgttaa atcaagcttt ggatatcata
    10921 ttattaaagc tgataaacca acagacttta acagtgaaaa acaaagcctg aaagaaaaat
    10981 tagtcgatca gaaagtacaa aaaaatccaa aattattaac tgatgcatac aaagatctat
    11041 taaaagaata cgatgttgac tttaaagatc gtgatattaa atcagttgtc gaagataaaa
    11101 tcttaaaccc tgaaaaactt aaacaaggtg gcgcacaagg cggacaatcc ggcatgagcc
    11161 aataacacaa aaccgagcga ccgtggttta aaaatcatac cacggccgct cggttttttc
    11221 gcattaaaaa tcggacagat gagctcatgt ttcagtatac tcatctgtcc gatatctttt
    11281 aattcttaat cgagtgattc aggattgtag aatctacgat tttcaagacc aaatatttta
    11341 tctgtaaact gacccttgtc agttttttta taagcctttt caaacatatt cattctagca
    11401 tcgatattat cgatatagca taaaatttct gcttctttta agtatggcag ctttggcgaa
    11461 ccatactcta acttaccatg atgagataaa atcatatgtc ttaacaacat gatttcttct
    11521 ccttcaatgt tcaattcacg agctgcttca actacttcat cactcgcaat cgagatgtgt
    11581 cctaataaat taccttcgac tgtatacgac gtcgcaacag gaccactcaa ttctctaact
    11641 ttaccaatat catgcaaaat aataccacta tataacaaac ttttgtttaa caatggataa
    11701 atgtcacaaa ttgattttgc aatacgtaac atcgttaata catgatagct taagccactc
    11761 gcaaagttat gatgatgaga actagcagct ggatatgtgt aaaatcgttc ttgatatttt
    11821 ttcaataaat gacgtgtgat acgttgtaaa ttagcatttt caatatctag caaataatga
    11881 gaaatctctt cttgtatttc tgccggtgat aaaggtgcac catctacaaa ttgttctgtt
    11941 tttaattgat cttcagttgt cgctagtcta atttggttga ctttcatctg tttatttccg
    12001 cgatagttta tgatgtcacc tttaacatgt acaatttctt caggcttgat tgttgccata
    12061 tcattttttg tagccgtcca aaatttcgct tcaatttcac cacttttatc ttgcaaatgt
    12121 aatgtcatat aatctttacc ttgtgctgtt acaccctgtg tagctttatg cactaagaaa
    12181 aagtgatcaa ctgaatctcc gggatttaga ttctctatat ttctcatcgt ttcccgcctt
    12241 cctctatttt gtttaatgta atcacttctt ttgatggaac aatattatct tttacacatg
    12301 taaagtatag tacttgatag tgttctgata atgatcgtaa ataattcaac attttttcag
    12361 tacgtttttt atcaaaatga acaaatgcat catcaacaat taatgggaac ggataatatg
    12421 gtcgtagtac cttaattaaa ctgatacgta aagctacata aagtaattct tttgtagatt
    12481 gacttagttc aacaggatca tataattgac cattaacatg tttaaccgta attgaatctt
    12541 cattatagtt aatcatcgta tatctgccat ctgttaaatg cttcaatatt tctaccgctt
    12601 cattaataac ttgaggcaaa cgtttatctt taatttgttt aatgtgttca tcaactaaac
    12661 tttgtaaata acttaaactt gcccaatctt ttgcgatatc attaagttga tttttaagac
    12721 tgtgatattc atgtcttaaa ttagcaagcg ttgtatcagt ttccatgtga ttgatttgtg
    12781 cacttaaatc actgacttgt gcttgcattt caagatattg ctcattatat tcgtcaactt
    12841 gagtagccaa taaatgatct tcttcttcaa gttgtgctgt tgttttttca cttaaactag
    12901 aacttaattc ataagaatag ttttggttct caagatattt tgttaaatca ttaaaacgac
    12961 tcaaattact agtgtatgtt tggtaatctt catgatgttg gtaaaaatct tcttcagtac
    13021 caacattgat aaaatcgaat agtgctgtaa tttctttatt attttcttct aattgagcat
    13081 ttaaatgatt taattcattt gtaacaagtt tggtattttc agcattaata cgccattttt
    13141 cattcgtgtc ttcagctgat ttcaaccatt gttgcacatc gtggaataaa gataatttgt
    13201 tgaaataaac aaattgtgat tttgttacag cttcagcatg attgtagaat gtatctaatt
    13261 cttgaaccaa ttgctggcgt tgttgattta aatcactgat atgttgatct aatgctttaa
    13321 tattcgccat tgtagaaata ctatcaacaa ttaaatcatt tgaaatttta gatgataagt
    13381 ataattcatc cttaacgttt tcaactgtcg attgtaattc atcatgacgc cctttcgcat
    13441 catttaaacg accttcaata tactgacgtt tctcttctaa aatatcttta tttttcaaag
    13501 cttgttgcca gtgatcacga atgcgatatt gctcatcaag atcaaaatct aagtcatagt
    13561 tttcatctaa aatggctagt tgtgctttaa ttacttcgat ttcatctgtg atggcctcgc
    13621 tataatatac ttcttttgat ttagacatga tgataccgat aacaaatact aaagttaata
    13681 ctgcgaaaat aataccaaac aacatgttgt ttgaaataaa tgagaagaca gttaaaccaa
    13741 tacctactaa tgttaaaaga ataaacgttg ttcgtaacaa tttttgacgt ttttgttttt
    13801 cttgttgctc aatttcgaaa cgttctttca acttgctata caagttctct ttttcgttta
    13861 attcaatgac ttgttgtgag tattcttttt tcttttcaaa agtctcttca ggaactattt
    13921 tttcttcaac agaatctagt tcgctatgaa ccgcattatc ttcgatttta ttttcttcta
    13981 aactacgttc taattgctta atgtatgccg cttgttcttg tttattcttg atttgctcac
    14041 tgacataact tttcattgcc tcagagctat ctacgtcatg atgcgtttca gaccaaccaa
    14101 tatttgcttg caattcatct ttatcacgtt gtttattcgc aatatccttt tcgattgcag
    14161 taagttcaaa ttctttattt ttaatttcat tttcttgttg attcaaacta atgaaggcgt
    14221 caatatcaga ttgtttaact ggctctaatt gagtcgcttc ttctttaagt tgagctaaac
    14281 gctcatttct taaaccaata tctctttcta acgattgctt atgcgctcgt gctttttcgt
    14341 aacgatctac acctttttct gggaatgtga ttggctcaat atttaactgt tgttctagag
    14401 acttccattc ttgtgaatga tcatgtaaag caacctcttt ttgtttttct tcatgcattt
    14461 ttgataattg atttaaatta tgctttaaat tctctaatcg acgtgatgat ttatctcgat
    14521 catctactaa acgatgatat gtttctagtt tagcttcttc ttcacgaatt tgactttcta
    14581 gttgttttaa ttgctcaatt tgttgattaa taatcggatt tttacctgat tttttatata
    14641 attcatcttt tttacgatta atcacttcgc gcattgacgt gaattcagtt gatcctaaag
    14701 cccctgcttg taataaataa tcttgcaatt gtttttcatt tagatttcta tgaatgtctt
    14761 gaagccctag tacatcaaat gaaaagatac cttgatatgt ctttttagaa atataattaa
    14821 gtttcttttg taaccaagca tcatcacgca cagcaccatt aggtaaatat actttcacat
    14881 caccttgagc actgccttta attcgttcaa cttcaatctc taagccatca tcaagaataa
    14941 gtactaattt accaccgtat tggttaccta gacgtggttc tagtcttggc tctttagact
    15001 ttttagttgg aaaaccaaat aatatcgaat ggatgaatgc ttgaatcgtc gatttacccg
    15061 cttcattttc accaaaaatt tcagtgaagt ttttattaaa ttcaatttta cgttgaacaa
    15121 attgaccgta accataaatt tcaagtgatt taattatcat tttgttcacc tctcatttca
    15181 gcttttaata tttcttcagc acgattaact aatgctgtat ggtcgaatgt tccataatcg
    15241 tctaggaact tagatgccct tggatttaaa tataagtctg acatcgcttt atcaaaaaca
    15301 gtttgatcga ctaataattc cgctgaaaat tcattaacta aaggtgactc atcattttgt
    15361 gcatattgta tttttaactc atcaatatat acaaattgat tttcgttttc ttcataatct
    15421 gtaatcattt cttcaacttg taataaatct tgaggtgaaa ttaatgtctc actattaata
    15481 acaagcgtta aacgataaaa ggctttccct tcttctctca cttgttcttt aaagttttga
    15541 atgacctcgt ataaaccttg cttagatgtc ttatccgttt caatagttgc ttcttcaaat
    15601 ctaatatact gtgtaggata aaatttagtt tttaatttta agtggtcacc ctcgattaat
    15661 aagcaacctt tttcaccttg ctcattaaaa tgtctacctt gaatattacc tgaatagtta
    15721 attacaggca tatcacttaa ttgttgacgt tcatgtatat gacctaaagc ccaataatga
    15781 tacaatttac tgtttaaatc ttctaaaatg aactcggtat atctttcgtt aactgaagat
    15841 ttactgtacg taccatgcaa gacaccaata tgtatgcctt tttggccttg acttgatgga
    15901 tattcatcaa tcttgttctc ataacttgct ctattttcat aactaaatcc gtgaatataa
    15961 attgtttcac cagatttagt aattgcttca tacgtctcaa ctttatttga aaatacagaa
    16021 acattatctg gccagtttga tgaaatcttt gatgataatg ggtcgtgatt accatgacaa
    16081 acataaacaa agatttgttc attttgtaaa cgttcaaatt gctgttttaa gaaaatttca
    16141 gcacgtaatg ttctgttttc actatcaaac aaatcacccg cgataattac aaaatcaaca
    16201 tcttgttgta atgcaatatc tacaatgttt ttaaagcttt cataagcact tttttgaaca
    16261 tcctcaaaaa ttttagggct tatatgactc ttagatttga aaggactatc taaatgtaaa
    16321 tccgaacaat gaataaattt aaccatacat ccatatctcc ttaattaaag gttctaacat
    16381 caattttgca aaatccaact atacaaagca ttctaatcac tatattcata cttataggtg
    16441 tagtgattca ttagctgatt tattaaaacc agtcgccatt tttattttta tttcgttaaa
    16501 aaattattat ctacacaata ataaagtgct ttagtcaaat cgtgatgttt tatttattaa
    16561 caagtttatt atttcttcat tttaccataa tacgcttcat atcgtcgatg aacatatgaa
    16621 attgagacac atttataaca gtttaaaatt ttatttgtag cgtgcgttga tttttttcaa
    16681 aaatttcagt cgaaaatgta atttccgtaa ttgttaacac cgctagtgaa ccaactgata
    16741 cattaaagat ttacatgatt actctactag atttgcttgg cagtaatgac caattagaag
    16801 ctaaatgtca aaactatcat tgcacttaat acgccaaaca ttttcaagcc gttcttaatg
    16861 agtcattaaa agtgaaacac ttttccatca gtccgataca tgaccgaact aatagtatta
    16921 tgctcaaaat tttacactcc aaaatgcaaa aaggaagtta ggacacgttt actagcctaa
    16981 cttccgttat caagctcatc cgcccgtttt ggcatagagc caatattgtt tgattgccca
    17041 gcttcagtcg tccgttctga gctaaagtta agacatgttt aataacctaa cttccgatat
    17101 tgaacgcatc tgcccaattt gacagggggc ctccaacttc cgttatcaaa actaaaaaaa
    17161 ctacccacaa agtctaggac tctgtcggca gtttattcct ctatttaaag gattgaaaaa
    17221 tattatttta atattagtca gcgtaaattt cgtctaatgg tttaacgata atttggttga
    17281 tttcttggaa tacttgactc attttttgtt cagcattcat taatgcagag atgttttcat
    17341 ctttttcaat tgcttgcgct tgttcttgcg ctttttgtaa atcttcttca gcaatttctt
    17401 caccttgcat ttgtttttgt tggaagttaa tttgagtttc acggaactcg tcgaataact
    17461 ttttagattc ttcgttagct tttacattag cgaatgcttc tttgattgct ttgtattctt
    17521 cgctttctct taaagcttgt tctaattgat ttgcataatc atataaattt actgccatgg
    17581 ttatagcact cctttgttgt gatgtttatt aaaacgttcg atattacttt accacatctc
    17641 gcttttttag acaaagattg caaccaaacc ttggaaaaat ccaataatac cacctaaaat
    17701 gaaacctaat gacataatca actttagttc tttgttagca atttctataa ttaatttttc
    17761 aatataatct aagtcaaacg tattaatttg ttcttcaatt aatcctcgta aatccacttt
    17821 tttcataatt gtagataagt gaatcgataa ttgttcaatg attaaatttg ctaatttgct
    17881 agagagttga ctttctaaat aatcaacaaa ttgaggcatt aacgtaacca ctggcttatt
    17941 cgcttgtttg cttgcatatg ttgttacata cattgataga ttttcagcga tttcattaaa
    18001 ctgcgacgta tctaacagtt tattcaatga tttatcttta aaagtttgat attcattggt
    18061 aattaacgat gtcacaattg ctcttgcttt aggatgagat gttagacgta taagttcttg
    18121 ttgaattcga tctgcaatgc tctcttttgt cataaacatt tgcaacatac caattaactt
    18181 acctttctca ttgaaaaaag tatccaacat atcattaata tcttgtgtgc cttttgcaga
    18241 tgataaataa tttcttgcac gatcacataa taagtctgtt gcgttatcta catgctgatc
    18301 taaaaatgtt acaagttgat ttggcaataa agatgctatt gtttggtttt gatgctttgt
    18361 ataataatta ttcaattgtg atgcaatata ttgatttcca ttagtttgta atacttgttc
    18421 taaatcaata tcgatttgag aagtaatttg tttaattgac aattgatctt tcgtcaactt
    18481 ttgtaactgc tgttgaatca tagattctat tgcttgttgt gattgctcgc tttttaattt
    18541 ttcattaatt aaagtttctg taagcaaatg ctcttcaatc acttggccaa ttttagttgc
    18601 aatttcttcg cgtcttttcg gtattaaacc tggtgtaaat gggactctaa atttaaatat
    18661 atagtatggt ttaaaggggt gaaacagcat tctaattgca attacattag taacaccacc
    18721 aattatcgcc cctactacaa tcataaatat gataataaat agtgcattca taattttgac
    18781 tcctttatga caacatagtt attcattata ccactatttt tattttttaa ttcattaatc
    18841 atttcacttt ctgctcaatt tattattagt ttacatacaa aaagcgatgc ctcacctctc
    18901 gaaacaggtg tgttacatcg cttttattat ttattaaaag tatataggcc tagatatgta
    18961 ctaattcttc tttagcattt ctgttaaagt atgtttcagc ttctctcatt ttagaagtac
    19021 cgaaaattgg ttggttatct ggatttaata cacgataaat attgcttact ttgttacttt
    19081 gtaaaatgta cccattacga gtctcaatgt tattccaata aatatcactt gttggtgcat
    19141 ggtttggata tgcacagtga tttcttgaaa ttgtttgaac gatttctaat ggatcacagc
    19201 caaatgtact gtttaatact tcagagtctc tgaataatgc acaaattgaa atacaagttg
    19261 tccagtttgg taatactctc tctttttcga tttgtactaa agtcttttta gaaagtccaa
    19321 ttgtttgcgc catagtatct tgcgtataac cagcctctat acgaaccatt ttaaattttg
    19381 tttgaattaa atctgtaaaa ctctgtctat ccattctgtt atctaccttt ctgtttgggg
    19441 aattttatcc ggacacaaga aattgcaata atacacattt cttgaaacac agattacatc
    19501 ttaatatatt tttaataaaa tgaaaagagt caatttcaca tttgtattaa attttgatca
    19561 agtcaacaaa agttaagttt acttcataat gaaacaaaag taaacgaagt taaattttat
    19621 gcattatgtc tacatttgat aaaacttaca cttttattat gatatcaaat ttacaaagaa
    19681 aactgtataa taaatgctat cgctgtaaaa aaaatctaaa taaatcgatt aaaaattgtc
    19741 gattttttga cgaagctaca tcaacgcaac tttaaatcaa cttattttcc attgcataaa
    19801 ttgctgcttg tgtacgatcg ctaacttgta atttactaaa tatatgactg acatgtgttt
    19861 taattgtttt ttcagataca aataaagttt ctgctatctc tttatttgtt ttacctttaa
    19921 ccatttcacg taacacttca atttctctct ttgacaactt attcgtgtag tgtggttttt
    19981 ggctaactgt ttcgaataca tcttgtgcct taggatgtat cattttttca ccgttcataa
    20041 ctcgtctaat agtttcaatt aattgctgag gctcaacgtc tttcatttca taaccatcag
    20101 cacctttatt gattgctgaa attacatgtt catcatcaac ataacttgtt aataccaaaa
    20161 ctttaatatc cggataatgt gccttaatat attccgtaat ttcaatacca ttcatgccag
    20221 gcatcactaa atctaatagc acaatatcag gatgctcatg ctcttttaaa tattctaaaa
    20281 atgtttctcc atctgcaaag tcttgtaaaa cttctatgtt ttcaatcgtg gataataaaa
    20341 atcgcaatcc ttgtcgcaca atataatggt catctactaa tattactttg ttcatgggtt
    20401 atctccttaa atcaagctat tttataggaa ttgtaaattg tatttgtgta ccctttgttg
    20461 gctgagaatg aaaggtcact ttacctctta ataatttaac tctttgtttt atgttattta
    20521 taccgtgtga tgaagctatc tgaacattat cgatctcaaa tccttgacca taatcaatca
    20581 cgtcaatata tagtatatcg ttcatttgtt ttaatgtaag atccatttta ttcgtatcag
    20641 catgtttctt aacattatta atacactctt gtaatgctct atatatgttt tcttcgattt
    20701 cattagataa atcgattaaa ccttctacat ttacatttaa ttgtatatgc attaatttac
    20761 tatatgctgt caaagcatga attaagcctt gctcaagtcc aactggctta agttgccaaa
    20821 tcaatgcacg catttcatta acggcatttt gactcgtttc ctcaatcgtc ttgaaagctt
    20881 gtttagcaat ggattcgttt gacatgccat acgcagcatg tgctgttagt tttacagaaa
    20941 ataacatttg atttactgaa tcatgtaaat ctctagctaa acgattacgt tcattaattt
    21001 ttgctgcttc tttttcacgg tctgttaaat aaatacgttt gatggctgac cctaattgaa
    21061 atgcgacaga ctctagcaac tctaaatctt catcactata aatttcagta tttggtgacg
    21121 ctacatttaa aatgccgaat tgttcttgac ccgatttaag tggtaccgtt gcatgatgtg
    21181 taatattgtc attttggcta ggaaatgctt tagaggctaa gttaatacga gaacaattga
    21241 cgatattcga cgctttcatt agcctacgtt gattaaatgc tttcacacac caacaagacc
    21301 catctttaat atagtgacag tggtctgctg tcaaagattg tggtaaagcc acatgtgata
    21361 caagttcatg ttcacctacg ctattgatga aaaatatcca gcctgtcgtg aaattactgc
    21421 cctcaattaa atattttaac gcaccttggg tcatgctata catttcagtt tcttcattta
    21481 aaaattcggc aatctctttt aataaagcta gtcgcgtcct ttgttccatc aaatcgctcc
    21541 aattcatttt tacgtgtatt aactattata cattgagtta ttatattttt aaatcttaga
    21601 cgtgaacatg ataaaatggc cttgattact taatagttat atttcggaga actgatttgt
    21661 gatatgatat taaagactat aggaggattt tatgaaattt aaaataccag aaaactttaa
    21721 tgacttaagt ttacgagata ttttccaaca acttaaggta cctaaaaaag atttacatca
    21781 tttaaatatg tctaaagata ttactattaa tgataaacct gcgcgattaa tggataaagt
    21841 gcatactggc gacgatgtat ttgttccaac catcgatgaa aaaagtaatt atgttccaag
    21901 ttatcgttat gcacaaatta aatacgaaga tgatgatatg gcaatcgtaa tgaaacctaa
    21961 aggtgttaag acccacccta atgatttaaa agaaagcaat actttaatga atcatgtgat
    22021 ttacacggtt gatagcgatt atgtcgaacc aattcatcga ctggaccagg aaacagtagg
    22081 attattaatt gttgctaaaa atcctttaat gaaaaaaatt cttgatcgca tgttagaaga
    22141 caatgatatt acgcggatat acaaagcaaa tgttaaggca cttttacctt taaaaccaca
    22201 aacgattgat atgccaattg gtaaagataa attccattcg aataaacgac gtgtgtctcc
    22261 tactggacag cgtgcaatta cacacatttt aacttcaaaa atgataaaag aaactgtgtg
    22321 ccaacttgaa atcaagttgg atactggacg tactcatcaa attcgtgtgc atttagctga
    22381 aattggtcac ccagttattg gtgatccttt atacggtgat tcaacgttaa gacaattaga
    22441 acttgaaagt tacaaaatag agtttgtgca tcccttgact aaggaagtca tttccgtttc
    22501 tttggatgac taatttgttt agtttggcat gatatttaaa tatgcaatac tgcattgtaa
    22561 ctaaatcaag tatcaactta aatcggatag atggaaaatt attaattttt tcagatgttc
    22621 ggttttttta tgttttttac gaccttagga ttttatattt tgaatgtatt tggatttata
    22681 gcatccttgg ataatgactt gtagtctttt tcaactgcgt tacgtgtatc taaggacaat
    22741 acatgacatc ataagatttt tatcacatat tgtttggtca atacatgtac aacaattcat
    22801 caaataaaaa ataggttcta taataaaatg gaatccatga aaagtttttc ctttcatggc
    22861 tctatatcaa atcggactta taagttcaat ttgacataga caaaaaaata aagcttgaac
    22921 actacatcta cctgtaatgc ccaaacttta ttttatatta atatttaatt aatgaggatc
    22981 taccatatct tctggtttaa tccatgcttc aaattgttct tctgtaacat atccagtttg
    23041 aattgcagat tcttttaaag ttaaaccttc tttatgggct ttcttagcaa tttgagcagc
    23101 tttttcataa ccaatatgtg gatttaatgc agtaactaac attaatgatt gatttaaata
    23161 attatcaata ttctcttcga ttggttcaat gcccactgca caattgttat taaatgtttc
    23221 cattccgtca gctaaaaggt aaattgattg tagtgtatta tgcataataa ctggtttata
    23281 aacattcaat tcaaagttac cttgtgaact tgcgaagcca acaactgtat cattacccat
    23341 tacttggact gcaaccattg ttaacatttc acattgtgta ggattaactt taccaggcat
    23401 aattgatgaa cctggttcat tttcagggat agaaatttct gccaaaccag ctcgtggccc
    23461 tgaagccaac catctcacat cattagcaat tttcattaag tctcctgcta gcgccttcaa
    23521 tgttccgtgt agttgtacta cttcatcatg tgctgttaat gcatggaatt tattttcaga
    23581 agatacaaat ggataacccg tattttcaga aatatactgt gccactttat caccaaactc
    23641 aggatgtgca ttaatacctg taccaacagc tgtaccaccg atggcaagat ttaaaatgtg
    23701 cttcttagat tcagataaca ttgtttcgca acggtcaagc atataacgcc agccactaat
    23761 ctcttgtcct agtttgatcg gcgttgcatc ttgtaaatgt gtacgaccaa tttttataat
    23821 tgaatcaaat ttatcttctt tttctttcaa agtatttctt aaaagtttta atgcaggttc
    23881 taattttgtt tcaacctctt gatatagtgc aacatgcata gcagttggga atgtatcatt
    23941 cgaactttgt gatttattta catcatcatt tgggtggata ctttcatcac tatgatgatc
    24001 ttttaaatac atattagcaa cataacttac tacttcgttc acattcatat tactttgtgt
    24061 accgcttcct gtttgccata caactagtgg gaagtgttca tctaattcac ctgataaaat
    24121 ttgatcacat gcgtatacaa tggcatcttt ctttgcctcg cttaattttc ctaaatcaaa
    24181 attagctaat gctgctgcac gctttagttg tgcaaaacca taaactactt cgattggcat
    24241 acgctcttta ccaactggga aattacgttt acttctttct gtttgagcac cccaatattt
    24301 atctgcaggt acttctattt ctccaaaagt atcatgttca attcttactg acattcaatt
    24361 tctcccctta tcactgttta tttaactgta gtatatcatt aaataattta attgagcaat
    24421 ttatgattaa aacgttttca taatttgaaa taaaaataca ctaatcgcac gtgcccaccc
    24481 tttattacag tgatacggtc atacgattag tgtgttatct atcattattt agttaatgtt
    24541 gaactaagtt taattacgat actttgtttt agtagcttca accgtagcaa tagctgtaag
    24601 tatatataat acagcactaa caattgtcgt atatggattt agagcaacaa gcgtacctaa
    24661 tactcctgtt aaactcgcat aaaacccatc aagtgtataa tacaatattc ctgttattaa
    24721 taaacaacaa attacactta ttacaatacc tgattgatta cttttaatga atgtttgcgc
    24781 attaacatca tcgattaatc cttttgataa attgagttgt aattttatta ctttgaaaat
    24841 aataggtaaa tataatgccc caattgccaa tggaaatgct ttaattgata ttaaacttat
    24901 aataactgtt gctatcaata attgaatcca gtattttcct aacataaata tataaatctc
    24961 ctctaatttc attcttcaat agcatatcat aatcttggca tattaagaaa cgcggtttaa
    25021 tgatttcatt aaaaatatta ctgatagatg acttctttca attatgtctg gagtaattaa
    25081 ttatcaattc tgtttaaatg gtgttttaaa atttaaaatt gaatttttga tatattacta
    25141 tgtctagtac acaaatcaat attctatgct ctacaaagtt atattggcag tagttgactg
    25201 tagtccccaa cattgaagct tcggcatgtc agcttactat ttcatgcaag ctggcggggc
    25261 cacaacacag tggaattgga tttccaattt ctactgacat tgcaagttgg ggaacgaccc
    25321 caacatagtg aaatcggatt tccaatttct acagacattg caagttgggg aacgggtcca
    25381 acatagaagc tggcggaaag tcagcttctg tgaatataat aaaaaagcta ggtaacaaaa
    25441 tgctacctaa cttcatattc aagattatca atcctatttg atataggttc tatactattc
    25501 attgtttaca tgataaatag ctgtatatta cacaattata atactttact gactgtcttc
    25561 ttcagaattc ttttcttgat cattttgatc agaaatttgt tccatttctt tacctaattc
    25621 ttttaaatct tcaaaatcag ttaccatact gttttcttct tcatgataat ttaattttgg
    25681 atctttgtct ttagacataa tcaatacctc acagattttt aattaagcaa agcgtgacgt
    25741 aaagtaagct ttaacatctt cgggtaaacc tgcagccgct tctttatcaa gaataacatt
    25801 taccattcta tgtgctttta aatcggctgg ttcaaagctt gttttaccat tttcttgata
    25861 taatttttca actacatctc gtttattagc accagtcact actaagaaaa tttctcttgc
    25921 ttccattagt ccttgacgaa tactaacatt taacttacct tgctcatcga tagaaacaac
    25981 ttgtaatgtt aatttccctt tattttcttt agttttaatc ttatcagcga ttaattcgat
    26041 tgcatctttt tcataagcaa ttggataaac ttgacctgct ggtacaccta acgcttcgaa
    26101 atatgatttt ttatcgtcat aatctaaaat atttatttgg ctaaaatcaa cagcatgttt
    26161 ttcaatattt ttctttaatt catctagtac tggcgcttga tcagtatcta aatgaaaacc
    26221 tgcaattgta gtaggattat tgttaaattg ctttctaata atatcagcag catattctgc
    26281 tacaagttga ctattgtcaa agactttaaa gttcattgcc atgatttcta cactcctctt
    26341 agtttgcatt ttacataaaa acggcgatat taataattat tataccctaa ctttcaatat
    26401 atcaaaccat ttaactttaa catgcttata ctctaaatat agcacttaag catcattttt
    26461 ataatgaaaa tgagtaaatt ttaattcaat cctggaaaat cttgttgacg taacgcttca
    26521 taaattaata acgcagcagt atttgataaa tttaatgaac gaatatgttc actcatagga
    26581 attcttaacg ctgtgtcttg atatttctct ttcacccagt ctggtaatcc tgtcgtttct
    26641 tttccaaaaa tgaagtaaaa atctttgtca tgatttgaaa aatcaaaatc actataagtc
    26701 tttttaccaa attttgttaa taagtaatac tcgccatttg tggcttcaaa aaatgcttca
    26761 atactatcat gatacgtaat attcacaaat tcccaataat ctaaaccggc tctttttaac
    26821 attttatcat cagttctaaa tccaagaggt ttaattaaat gtaaatgtgt gtttgtacct
    26881 gcacacgtac gtgcaatgtt accagtatta gctgggattt ctggttgata taaaacgata
    26941 tgatttgtca tattactatt ctctccttgt gtccaatcct tttatcattt cattctgaac
    27001 ttctgcatcc tcttgatcgt aatttgcatt gataaaatct cttgcttctt ccccaagaat
    27061 ttgaccaata gcccaataag cagttgctcg aatcatcggt ctttcatctg ttgttgcaac
    27121 ttttttcaat tctggaattg catccacttc attaaaatgc gccaatgcta aaatagcatt
    27181 tcgttgtatc ggttttttac cacgccaagc acctgcaagg tgaccatatg tttgtttgaa
    27241 ttctttatta gacatacgta gtaaaggtac taatcttggc tttaaaattt ctggttccaa
    27301 aatgatgtca tcttgttcgg tattaatacc tctatttttc gggcaaactt gttgacacgt
    27361 atcgcaacca tataatctat tcccaatttt ataacgatat tggtcaggca tatagccttt
    27421 tgtttgcgtt aaaaaactaa tgcatttctg actatttaat tggccatttc caactaatgc
    27481 acttgttgga caacgatcaa cacaaattgt acaatcacca cagctatcta ataatggatc
    27541 atcaggttca aaaggtatac tgactaacat ttcaccgagg tatgtccatg ttcctagttt
    27601 aggattgatg acaaagccat tacgaccaac aaatcctaaa cctgcacgtt ctgctactgc
    27661 cctatctgat aatacacccg tatctaccat agatttgatt tcaacatctg gaactttaga
    27721 ttcaataaat gcagctaaca tatctaaccg tttacgcata attgtatgat aatcttgacc
    27781 ccacgatgct ctagcaaata agcctctgcg atcacctcta acactcttag gtgcaccttt
    27841 cagtttgtta ggataaccaa ctgcaattgc tatgattgac cttgctgttg gtaaggataa
    27901 tttaggctcc gttcgtaaag caatatcaga ttcttcaaat cctgaggcat aaccatttgc
    27961 atgatatgct tctagctttt gcttcaattc atcaaaggga tcggcagtag taaatccaat
    28021 actgtcgata ccaattgtat atgcatagtc aatgatatct tgctttaact gctttgtatc
    28081 caatcgtatg ccccccataa aataatacta tgttatttta acacaaccat tattcatatt
    28141 gaggtttcat ttcacataat tttaataata aaaagcttga atatatgata taactttgtg
    28201 gtgcgcatca tatattcaag ctacgttagg ttgttataat actcttgcta agaaattttg
    28261 tgttctttca tgttgcggtt gttcaaatat ttcgactggt gtgcctgact cgacgacaac
    28321 gccatctgcc ataaatatga ctttgtcact tacatctttg gcaaatccca tttcatgtgt
    28381 cacaaccacc atggtcatac cttctttagc taggtctttc attactttta atacatcacc
    28441 aactacctca ggatctaatg ctgaagttgg ttcatcgaat aaaataacat ctggatgcat
    28501 tgctaaagct cttgcaattg ctaccctttg tttttgacca cctgataatt gattcggata
    28561 tacatctgct ttttctttta atcccacttt atctaataac gacaatgctt ccttatgtaa
    28621 ttcatcgtta ttatctttct ttaataattt aggagctaaa ataatattat cgacaacttt
    28681 tttatgtgga aataggttga agttttgaaa taccataccc attttttggc gtagtttatc
    28741 tacttgtgtc cctttttccg ttaaatcatt gccttcaaaa atcacttgac ctttagtggg
    28801 tacttctaat aaattcatac atcttaacaa tgtactttta ccactaccag atggacctat
    28861 tattgctact acttcccctt gattgatttc aagattgata tcttttaaaa cttcattatc
    28921 tccaaaaact ttattaagat tgtttatttt aatcactggc attcaatctc ccttcaatca
    28981 tgttcataat gcgtgtaagt acaaatgtta agacaaagta taatgctgct gccactagta
    29041 atggtgtgaa tgggtcaaat gaaatacctt gaaccacttg tgcattaaac ataatttcgc
    29101 caactccaat tgttgacaca atagatgatt ctttaattaa agtgacaaat tcattaccta
    29161 aagctggtaa aatgttttta attgcttgtg gcataattac acttttcatc gtttgtctat
    29221 aattcaaacc tagactacgt gcggcttcca tttggccttt atcaacagca tttatacctg
    29281 cacgaataat ttcagcaatg taagctgagg aattaataac taatgcaatt gttccacaaa
    29341 caagagctga aatgtctagt cctaatgcag ccgttatacc aaagaatacg ataaatactt
    29401 gaactaacat tggtgttcct cttaatattt cgatatagat agaggcaatc catgaaataa
    29461 tttttatttt acttaatttc attaacgcaa cgaatgcacc taaaatagaa cctaatgcta
    29521 caccgataag tgaaattaat attgtaatct taattccttt caagaaaaaa ctaccatact
    29581 tagaaataaa accactgtca tcattcatcg catttgcagc attagtcata tatttatcga
    29641 ttaatccttt atctttaacc tctttaatcg ttttattaat ttgtgacaat aattttggtg
    29701 aatcttttgg tactgctatc actgtatctt tttcttcttc attaaatttc acattggaaa
    29761 ttcctaattt aggattttgt tttaaatatg cttctgctac aggtttttca actacaacac
    29821 cttcaacctt tccacttttc agagctaata taacgtctgg taaacggctt aatgaagtaa
    29881 tagatgcatt ttcaatttcg gtttgagcga ttttttcttg ttcagttccc ttttgtgccc
    29941 ctactttttt attattaaag tctttgatat ctttataatc attaacttta tctttcttta
    30001 caagcatgat atttttagtc atcatatatg aatctgaaaa atcaacttgc ttcttacgtt
    30061 caggcgttga agtcattccg gaaataataa tatcaatttt tccagtttta agagctccta
    30121 acaaactatc aaatgacata ttgacgattt ttaattttaa attattatct ttcgcaattt
    30181 ttttagctaa atcaatatct acacctgcat actcagtctt accattaact gtatgctcaa
    30241 attccattgg tgcataatct gcagaaagac ccactctaag ttcaccgcgt tctttaatct
    30301 cctcccattt ttgatcttgt tcagcatatg ctgcgggatt gatatacacc atagcactgc
    30361 taattaacaa ccctaatact aatataaacc taattaaaca cttcatttat acagccctcc
    30421 ctaattaata ttcataatta tacatattaa tacattttga tgcaatagtg ttttataaat
    30481 gtatttttat caatataaag aaaagcttct ttttaaaaga ctttacttaa aaaatcgtct
    30541 attatacgcg aacaattctt aaattttaaa atcaatcaaa aaaagcgcta ctatcaatat
    30601 cttattgaca gtaacgccta tatttatcat atattaaaaa ttattcttgt catcaagcta
    30661 taaattattg ttttggaact tcaaatttat aaatatcatt agaatatata ggaatgaatt
    30721 ttttacacaa ttcatgaaca ataaacgccg atacaaatgg tatgacaaaa taaccaagag
    30781 ctaataaaat aatattcatt gttggatcac ctgacattcg gttaaatgca tttattgggc
    30841 caactaatcc tgtatatcca aatccagcag acaatggtgt tcctttaacc tgaagaacgt
    30901 aagcaatgat tccagtaatt attccattta tagtcaatgg tatcgaaata attaaatttt
    30961 tcaagtaaac tgggatcatc atttttgcag ctcctatgag taaaactgca tttacgccaa
    31021 tagaattgac acgcaatgag ccgaataaga aagtaacaca agcagccact atacctaggt
    31081 ttgctgctcc acttcctagc ccgtttaaac taatcgcagt tgcaatcgct actaacgata
    31141 ttggtgttac cattaataat gaaaatgcca cactaataag tatagacatt aacaacggat
    31201 ttaagtctgt aaaagaatga attacatttc caattgcttg agtaattttt cgaatgtaag
    31261 gtaatgtgat tagaccgata cccccactaa cgataggtac taaaactggt aatataatta
    31321 attcaaaaga tccaagcttg ttttgtaata ccatatatat aagacatgca ataataacaa
    31381 ccaaacttgt atttataatg tcacctatac cttttaacat aaaactatta ttgctatata
    31441 caacagcacc tgaaccaatc atagctgatg tacctactat agcagcacct gcgccattaa
    31501 atttaaattg atgagcagct aaaaccccaa taataaatgc cataaatgat tgaattagta
    31561 tcactaactg atacgttaat tctaaaattt cattaccact tttaaatatt tttaatactt
    31621 cacctaataa agcattcgga acaagtgcaa taacaacacc agcaccaata gaatttaaaa
    31681 tcttactgaa aaactgttta ttatcagcat tctttgcgtt actcataaac gacctccaat
    31741 ttgaacatta aactcatcat attatagaaa attaacttta acaatgatta gttggctaat
    31801 ttaatctaaa tataacaatt cacaaaagga ataaagtaaa ttggaacaat ctaatcagta
    31861 aacaatgtta tgttaatttt aaaatactac taattacatc ttgtgactaa taatgtaata
    31921 aattgtaaat attaaaatag ccatattact attaagatag ataaattgaa tcgacttggt
    31981 ataaagcaca ataaaatagc acttttacaa atattttatt agaaaagtta aaacaaatac
    32041 ataccccaga taatatagtc attatcatta atgtaatacc aaaaaaagaa gttaaacaat
    32101 gtatagttgc ttaacttcct atattaaact catcattaca atttgacata gagcctatta
    32161 aagcgtgtgc catttgagtg ccactttatt tgtattgtat agagagaaat aaaaagaaac
    32221 cttgttttac aaggtttcta gtacgttatg ttatgtaaat aacagttaat tataccggtg
    32281 gtcggggtcg aaccgacact ccacaagtgg aacgggattt tgagtcccgc gcgtctgcca
    32341 attccgccac accggcttaa tggtaaacaa aaaacttccc tttggaagca attatggagc
    32401 ggaagatagg atttgcacct atacctcgtt ccgggaagga acgtgttcta aaagttgaac
    32461 tactcccgca taaacctgga ggcggcaacc ggatttgaac cggtgataaa ggttttgcag
    32521 acctctgcct taccacttgg ctatgccgcc aata
//
LOCUS       DAMZPP010000010        29855 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMZPP010000010 DAMZPP010000000
VERSION     DAMZPP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 29855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29855
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.010"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..456
                     /locus_tag="POH73_000367"
     CDS             <1..456
                     /locus_tag="POH73_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6886347.1"
                     /translation="RNSLREWLPWVDATEQPSDTRAFIKSGLLQFADGNGFQCGIWYE
                     GTLVGVIGLHEINHMHRKTSLGYYLDKQFEGHGIMTQAVEALIKYCFDEIELNRIEIS
                     AAVNNEKSQAIPERLGFTREGMLRDNELLNGIYSSSYIYSLLKSEYNEG"
     gene            721..1575
                     /locus_tag="POH73_000368"
     CDS             721..1575
                     /locus_tag="POH73_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886348.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDITPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNKLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            1572..2801
                     /gene="efeB"
                     /locus_tag="POH73_000369"
     CDS             1572..2801
                     /gene="efeB"
                     /locus_tag="POH73_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDI6886349.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQSGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKSNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDTHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATQQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            2782..4494
                     /locus_tag="POH73_000370"
     CDS             2782..4494
                     /locus_tag="POH73_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HDI6886350.1"
                     /translation="MKHYLTKIIATIITLVMVFSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISKDNKLKAIEQVVSAVKKLSLEDNSEGNAVKSNVRKLEDAKANDDQKDT
                     LLQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSGTSANDQYHVSQLNDELEKAIKAIDNNQLSDADAALTHFIEIWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDINNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIVLAITFVETLGNSGMLRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYTNAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFVIGIALAIVILIVFALLFRFIVRLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQAAYIMVVAILIF
                     KFKK"
     gene            complement(4796..5497)
                     /gene="tatC"
                     /locus_tag="POH73_000371"
     CDS             complement(4796..5497)
                     /gene="tatC"
                     /locus_tag="POH73_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDI6886351.1"
                     /translation="MVHFSELRQRLVKILLSFVIAVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFS
                     VLLFCAGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGILFQL
                     PILFMGLAKFDLIDTYSLKHYRKYIYFACFVLASIIAPPDITLNILLTLPLILLFEFS
                     MFIVKFTSRNDLSSQ"
     gene            complement(5514..5738)
                     /locus_tag="POH73_000372"
     CDS             complement(5514..5738)
                     /locus_tag="POH73_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HDI6886352.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            complement(5837..6226)
                     /locus_tag="POH73_000373"
     CDS             complement(5837..6226)
                     /locus_tag="POH73_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDI6886353.1"
                     /translation="MAFKLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAPSSNQSIVQDVLTRHQQGQTDFETFCDEMAQAGIYK
                     WHIDIQAGTCTYIDLQEQAIISELIPQ"
     gene            6466..6669
                     /locus_tag="POH73_000374"
     CDS             6466..6669
                     /locus_tag="POH73_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6886354.1"
                     /translation="MRNRLKELRARDGLNQTQLAKQAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNEKVETIFIIEEDEV"
     gene            6666..7379
                     /locus_tag="POH73_000375"
     CDS             6666..7379
                     /locus_tag="POH73_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HDI6886355.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGLGIENLTFATHNNVV
                     VISIIATIIIILIEIIVLMNQRRALKYKRLVDEEVDNEETDQYELLANRHVLNGSILS
                     ILQTVIALLVLLIFVVGQAEVNGILLFLIPFFASAIFNTQFTLFNRRFDDRMPKIADK
                     NYTEKRLEILDEGEHHIELIALFKTYAINLSILILAIIFIGSYSIATGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            7404..8246
                     /locus_tag="POH73_000376"
     CDS             7404..8246
                     /locus_tag="POH73_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6886356.1"
                     /translation="MTTLLNLDRVNKQYKSSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFGEEHAIDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYMTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMELLEDFVAQGGGILISSHISEDIEQLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            8246..8875
                     /locus_tag="POH73_000377"
     CDS             8246..8875
                     /locus_tag="POH73_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDI6886357.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTGGWLVVIVYAVIGIVITSVSYISSIKIFNKQEL"
     gene            complement(9244..10377)
                     /locus_tag="POH73_000378"
     CDS             complement(9244..10377)
                     /locus_tag="POH73_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HDI6886358.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFATVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIASTGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLSSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISYSLGIL
                     VMSYIVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            10917..12098
                     /locus_tag="POH73_000379"
     CDS             10917..12098
                     /locus_tag="POH73_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDI6886359.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEINEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPAIMGIAPVDAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            complement(12181..12933)
                     /locus_tag="POH73_000380"
     CDS             complement(12181..12933)
                     /locus_tag="POH73_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDI6886360.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIETFENKDANGQQHAPGWG
                     LDALKCLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(12976..15204)
                     /gene="metE"
                     /locus_tag="POH73_000381"
     CDS             complement(12976..15204)
                     /gene="metE"
                     /locus_tag="POH73_000381"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDI6886361.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            complement(15201..17042)
                     /locus_tag="POH73_000382"
     CDS             complement(15201..17042)
                     /locus_tag="POH73_000382"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HDI6886362.1"
                     /translation="MSQFLTHLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAYLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLEKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKHTHFNIAGAFNPNVRKLDGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            complement(17011..18171)
                     /locus_tag="POH73_000383"
     CDS             complement(17011..18171)
                     /locus_tag="POH73_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HDI6886363.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAMSE
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTATHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTLER"
     gene            complement(18168..19271)
                     /locus_tag="POH73_000384"
                     /pseudo
     CDS             complement(18168..19271)
                     /locus_tag="POH73_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     misc_binding    complement(19337..19706)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            19935..20780
                     /locus_tag="POH73_000385"
     CDS             19935..20780
                     /locus_tag="POH73_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDI6886364.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGAKVDISIKKSVGKISFEFESQDDFVRIIKQLN
                     RKYGK"
     gene            20934..21815
                     /locus_tag="POH73_000386"
     CDS             20934..21815
                     /locus_tag="POH73_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDI6886365.1"
                     /translation="MDQVMSIISSLFEPLTKIETYESVATKLAMIIIYIIVALIVIKI
                     LDKMIAQGFKIQNKSKKGNKKRSKTLISLVQNVVKYIVWFIVVTTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFENQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVDENIDNVEKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAFEDTRVILRVSAETIPGEGFSGARIIRKEVQKMFLQEGI
                     KTPQPIMTPFNHSENGV"
     gene            21845..22048
                     /locus_tag="POH73_000387"
     CDS             21845..22048
                     /locus_tag="POH73_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDI6886366.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIVSQDDTQR"
     gene            22060..23157
                     /gene="ychF"
                     /locus_tag="POH73_000388"
     CDS             22060..23157
                     /gene="ychF"
                     /locus_tag="POH73_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDI6886367.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(23243..23434)
                     /locus_tag="POH73_000389"
     CDS             complement(23243..23434)
                     /locus_tag="POH73_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886368.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            23678..23974
                     /locus_tag="POH73_000390"
     CDS             23678..23974
                     /locus_tag="POH73_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDI6886369.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            23995..24498
                     /gene="ssb"
                     /locus_tag="POH73_000391"
     CDS             23995..24498
                     /gene="ssb"
                     /locus_tag="POH73_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6886370.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            24550..24792
                     /gene="rpsR"
                     /locus_tag="POH73_000392"
     CDS             24550..24792
                     /gene="rpsR"
                     /locus_tag="POH73_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDI6886371.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(25024..25818)
                     /locus_tag="POH73_000393"
     CDS             complement(25024..25818)
                     /locus_tag="POH73_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001800370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDI6886372.1"
                     /translation="MLYNTDNKKFKFLPNWLETTSYWFNMEHNNNINKKYKKFSRGSI
                     IYLDFGINIGHEFSGRHFGIVLNKNDSPYNSLVTVVPLSSKNKKHYLPLQNIVFKTSL
                     SILNKTVDNIEFDLDVINHVIFTVIEIINKNNLQNTLEQNTIDYLDDFAIKTRFPTER
                     EVINKSKNIVESFNLYNDPKNFSALFNYLLIDVNDRKSKVNNLREVINKYKKFDKNTF
                     ACTQNVVTISKLRILKINAEDPSGRMKLDFDSMKLVDNEIKNKLIN"
     gene            complement(25856..27070)
                     /locus_tag="POH73_000394"
     CDS             complement(25856..27070)
                     /locus_tag="POH73_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI6886373.1"
                     /translation="MWVREITKNNSTAYRYLERYTDPLTGKYKTVSVTRNKNNVRSQK
                     DAQLELNKIIEQRLKHYSTKQLENLTFHDACDEWLEHYKTHSGSKPTTIKEKKSNTNT
                     VKNAIDSKVLISKITHTYLQNIINEWAKSHSIGHVQSLVIVIRSVFKYAFKYYDLHDI
                     SVLDKIDIPKKAQTRNEFQAKRNNYLEDSEVKELLECFDYLIKHKRHATRKRNYEMVK
                     ALVEFQINNGMRIGELLAIKTDNVDVENKTLEIDGTINWVTDVVTGAFGVKETTKTSK
                     SYRTIGLTAQSINLLKKLMLENKKENQWNDKFIDRGYIFTNTAGSPIDLNKVNNIIKE
                     ATDISSINKRVTTHTLRHTHISTLAQLGINLKAIQERVGHSDYKTTLEIYTHVTDKMA
                     KDMMNKLEGIGS"
     gene            complement(27231..27854)
                     /locus_tag="POH73_000395"
     CDS             complement(27231..27854)
                     /locus_tag="POH73_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6886374.1"
                     /translation="MVESNDELKKELGRFLKSIRKQKGKTASEISKQMQYSQGHISGI
                     ENGVKSFPSNKLIESYLMNIKDTNEEYNFYVDEIAKITKNKVKLNKVSNVTNKMEIID
                     RMMDIPYSREFISFDDNNEKSFTIFNISINDLHFHLQDINNYKFYKGIRLTDNDKNNI
                     DKILNNYFENKSVIIKENTKTLRDKNENWEQLVKLSDYIDDKLDKKN"
     gene            28003..28167
                     /locus_tag="POH73_000396"
     CDS             28003..28167
                     /locus_tag="POH73_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6886375.1"
                     /translation="MFMTVKEVAQLLRISERHTYKLLQKNVIPHTKIGGKILVNKERL
                     LETLEKKEVK"
     gene            28168..28440
                     /locus_tag="POH73_000397"
     CDS             28168..28440
                     /locus_tag="POH73_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDI6886376.1"
                     /translation="MPRTKLQDFPSKENTVTEPEQVVVNPLFAKPNTLAGIFGISYSS
                     VNRILKEWEKDHKGINDLYYSLSSTMIVISIPRFEEYMKARHKKWM"
     gene            28452..28625
                     /locus_tag="POH73_000398"
     CDS             28452..28625
                     /locus_tag="POH73_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDI6886377.1"
                     /translation="MKMYLTYICLVSLLTILLLAISNMYVAFSVYGMMVTYGFNLTGE
                     ITTCENKLLLQKQ"
     gene            28592..28795
                     /locus_tag="POH73_000399"
     CDS             28592..28795
                     /locus_tag="POH73_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDI6886378.1"
                     /translation="MRKQVIITKTVVGWYNIKDTQHNLMLNIPPKVFEQYFPDVSKDV
                     QVACLEMDLSKITEIKNKKKVGS"
     gene            28797..29180
                     /locus_tag="POH73_000400"
     CDS             28797..29180
                     /locus_tag="POH73_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDI6886379.1"
                     /translation="MEIKQKYQLSKVVKILEVVLYEEDKFQSDKDYHYQDKAFYEYAL
                     KLVHNGLFNILAELDFEDEVFLILDEVTMALSDVMKETQHVYRYSVIDEKGEHKHTTD
                     RKGHVIGMLEWALDYIVGNIEVEVL"
     gene            29181..29507
                     /locus_tag="POH73_000401"
     CDS             29181..29507
                     /locus_tag="POH73_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HDI6886380.1"
                     /translation="MNWEINDLFSDLKLLKDRFEDLKDNHGWHFEELYPHEPNHNLNK
                     DELIREGASYHERRIHNNQMFDLFHLYIEQFDNIIEKFYEIEKASSENFGEESDDAKN
                     SIKVAE"
     gene            29572..>29855
                     /locus_tag="POH73_000402"
     CDS             29572..>29855
                     /locus_tag="POH73_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001667297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile element-associated protein"
                     /protein_id="HDI6886381.1"
                     /translation="MNEIKLEYDTHVSVVHYESLDSRSFKSFSKPKWSKLVNKLSVPI
                     EANYKYARGVAVYGDIKNGANDHGEIIKKHRNDKNVIYRDVIVLDYDEIN"
BASE COUNT        10031 a         4386 c         5609 g         9829 t
ORIGIN      
        1 agaaattcac ttagggaatg gttaccttgg gtagatgcaa ccgagcaacc atcagatacg
       61 cgtgcattta ttaaaagtgg acttttgcaa tttgctgatg gtaatggatt tcagtgtggc
      121 atttggtatg aaggaacgct agttggtgtc atcggtttac atgaaattaa tcacatgcac
      181 agaaaaactt cattagggta ttatttagat aaacaatttg agggtcatgg gattatgaca
      241 caagcagttg aggcattgat aaagtattgc ttcgatgaga ttgaattaaa tcgaattgag
      301 atcagtgccg cagttaataa tgaaaaaagc caagctattc ctgaaaggct gggatttact
      361 agggaaggta tgttacgtga caatgaatta ctaaatggta tttattcatc gagttacatc
      421 tatagtttat taaaatcaga atataatgaa ggatgataaa tcagactaca aaagagtgat
      481 gacttacata taagtgttac tcttttattt attttcaaac taaaaaggtt gttgacagta
      541 ttattttata acaatataat gattttgata attattatca actagataat gttcatggga
      601 ggatgcctta aaatcatcat tttaagagcc acgtattatc ttggcattta gggagtgtga
      661 gatagtacga atgagagcat gtctatgggg ataatagaat ttctataatg aggtgtcaaa
      721 atgaaaaagt taacaacgct attattagca tcaacgttat taattgctgc atgtgggaac
      781 gacgatagta agaaggatga ttcaaagaca tcgaaaaaag atgatggtgt taaagcagaa
      841 ttaaaacaag caacaaaagc atatgataaa tatactgatg aacagttaaa tgaattttta
      901 aaaggtacag aaaaatttgt taaagcgatt gaaaataatg atatggccca agcaaaagcg
      961 ttatatccaa aagttcgtat gtattatgaa cgctctgaac cagttgcaga agcatttgga
     1021 gatttagatc ctaaaattga tgcacgtctt gcagatatga aagaagagaa aaaggaaaaa
     1081 gaatggtcag gatatcataa gattgaaaaa gcattatacg aagataagaa aattgatgat
     1141 gtgactaaaa aagacgcaca acaattattg aaagatgcaa aagaattgca tgccaaagct
     1201 gatacattag atatcacacc aaaattaatg ttacaaggtt ctgttgacct attaaatgaa
     1261 gttgcaactt ctaaaatcac aggtgaagaa gaaatttatt cacatacaga tttatatgat
     1321 tttaaagcga acgttgaagg cgcacaaaaa atttatgact tatttaaacc tattttagag
     1381 aaaaaagata aaaaattaag tgatgatatt caaatgaact tcgataaagt gaataaatta
     1441 ttggataagt ataaagataa caacggcggt tatgagtcat ttgaaaaagt atcgaagaaa
     1501 gaccgtaaag catttgcgga tgctgttaat gcattaggag agccactaag taaaatggct
     1561 gtgattactg aatgacaaat tatgaacaag ttaacgatag tacgcaattt tcaagacgta
     1621 catttttgaa aatgttaggt attggcggtg ccggtgttgc aattggcgca agtggtgttg
     1681 gtagcatgtg gtctttcaaa tcaatgttca atacaccaga agatccggaa aaagatgcgt
     1741 atgaatttta tggtaaagtg caatcaggca ttactacacc cacgcaaaaa acatgcaatt
     1801 tcgttgcatt agatttgaag tcaaaagata gagatgcaat taaggcaatg tttaaaaagt
     1861 ggacggttat ggctgatcgt atgatggatg gtgatacagt tggcaagccg agtaacaatc
     1921 ctttaatgcc gccagtagat actggtgaat cgattggatt aggtgcaagc aagttaacga
     1981 ttacctttgg aattagtaag tctttgatga agaaaattgg gttatctagt aaaattcccg
     2041 atgcctttaa agatttaccg cattttccga atgatcagtt aatagacgat tacagcgatg
     2101 gtgatattat gattcaagca tgctcaaatg attcgcaagt atcctttcat gcggttcata
     2161 atttagttcg tccatttcga gatattgtta aggtacgttg ggcgcaatct ggttttatct
     2221 ctgctaaagg taaggaaaca cctagaaatt taatggcatt taaagatgga acaattaatc
     2281 ctagaaagag taatcaactt aaagattatg tgtttattga tgacggatgg gcgaaacatg
     2341 gaacttattg tgttgtcaga cgtattcaaa tacacattga aacgtgggat cgtactgcac
     2401 tggaagaaca agaggctaca tttggtcgca agcgacatag tggtgcaccg ttaacaggtg
     2461 ggaaagagtt tgatgaaatt gacttaaaag cgaaagatag tcatggcgag tatattattg
     2521 ataaagatac ccatacgaga ctagcgaaag aagcaaatac gtcaatttta cgtagagcct
     2581 ttaattatgt tgatggtacg gatgaccgca caggtaactt cgaaacaggc ttgttgttca
     2641 ttgcttttca aaaagcgacc caacaattta tcgatattca aaacaattta ggtagtaatg
     2701 ataagttaaa tgaatacatt acacatagag gttctgcttc atttttagta ttaccaggtg
     2761 ttagtaaggg aggatacctt ggtgaaacat tatttgacta aaattatagc gacgataatt
     2821 actttagtta tggtatttag ctttgggtta ttgaaaagtc aggcagcaga acaacaaagt
     2881 attagtgatg tatatagtgt gataacggat gcaaaatctg cactttctaa taattcgata
     2941 tcgaaagaca ataagctgaa agcaattgag caagtggtaa gtgcagttaa gaaattatcg
     3001 cttgaagata atagcgaagg taatgctgtc aaatcaaatg tgagaaagct tgaagatgca
     3061 aaagcgaatg atgatcaaaa agatacactt ttgcaattaa cgaagtcatt aattgcttat
     3121 gaagagaaat tggctagtaa agatgcgggt tctaaaatta aactgttgca acagcaagtc
     3181 gatgccaaag atgcagcgat gacaaaagcg attaaagaca aaaataaagc ggaattagaa
     3241 tctttgaaca atagtttaaa tcagatttgg actagtaatg aaacagtgat tcgcaattat
     3301 gacgcaaatc aatatggaca aattgaagtc gcattattac aacttagaat tgcaattcat
     3361 aagtcaccat tagatacggc aaaagtgtca catgcttgga caacttttaa atcaaatatt
     3421 gatcatgtcg ataagaaaag tggtacgtct gcaaatgatc aataccatgt atcacaatta
     3481 aatgatgagt tagagaaggc gattaaagct atcgacaaca atcaattgtc ggatgctgat
     3541 gctgcgctta cacattttat agaaatttgg ccatatgttg aaggtcaaat tcaaactaaa
     3601 gacggtgctt tgtatacgaa aattgaagat aaaataccat attatcaaag tgtattagac
     3661 gaacataata aagcacatgt caaagatggt ttagtagata taaacaatca aattaaagag
     3721 gttgttggcc atagttatag cttcgtcgat gtgatgatta tctttttacg tgaaggacta
     3781 gaagtgttat taattgtaat gacattgact actatgacgc gtaatgtcaa agataagaag
     3841 ggtactgcaa gtgtgattgg tggtgcaatt gccggacttg tattgagtat cgttttagca
     3901 attacattcg tagaaacact agggaatagc ggtatgcttc gtgagagtat ggaagcggga
     3961 ttaggtatcg ttgcggtcat attaatgttt atcgttggtg tttggatgca taaacgttcg
     4021 aatgcgaaac gctggaatga tatgattaaa aatatgtata ccaatgcgat tagtaatggt
     4081 aatttggtat tgttagcgac gattggttta atatctgtgt tgcgtgaagg tgttgaggtt
     4141 atcattttct atatggggat gataggcgaa ttagcgacca aagattttgt cataggtatt
     4201 gccttggcca tcgtgatatt aattgtcttt gcgctattat ttagatttat cgtcagattg
     4261 atacctatat tctatatatt tagagtattg tcgatcttta tttttattat gggattcaaa
     4321 atgcttggcg taagtattca aaagttacaa ttattaggtg cgatgccaag acatgttatt
     4381 gaaggattcc caacgattaa ctggttagga ttctatccaa gttacgaacc attgatagca
     4441 caagctgcct atattatggt agttgctatc ttaatcttta aatttaaaaa ataaaaaaca
     4501 ggccgagtgc ctgttttttt gttgctgtat tgtggatatc gaagcattgc agatatcgcc
     4561 gcattgttga ggtgcggata tcactgcatt gttggttctc gttgtattgc agatatcgaa
     4621 gcattgttgg ttctcgttgc attgcggata tcacggtgta atccgatgct gtattctgat
     4681 taacgcaatg ttagttagct gcagtattga tataatctga ggtgctacat tgttctttta
     4741 ttggctattg tgatgttgtt gcattgtgag tattgcagta ttgattcgta taaggtcatt
     4801 gcgacgataa gtcatttcga ctggtgaatt taacgatgaa catactgaac tcaaagagca
     4861 atatgagtgg caatgtgagt aatatattta atgttatgtc tggtggtgca atgatactcg
     4921 ccagcacaaa gcaagcaaaa taaatatatt tacgataatg tttcaaagaa tatgtatcta
     4981 taagatcgaa ttttgcaaga cccataaata atattggtaa ttgaaataga atgccaaatg
     5041 taaataacca acgtatgagt tcaatcaaat atgctttaaa gccaataaca ggcgaaatgt
     5101 ttaaagttaa tgataatttt aacgcgaatt gaatgatcat tggaaagcca acataaaatg
     5161 caaaagcgac gccagcacag aataataaca cgctgaaaaa actatattta taaataaatt
     5221 gacgttcatt attatgtaat ccaggtgcaa tgaatgccca taattgataa aacatgactg
     5281 gtgaaatgaa acaaaatgcg atgaaaaata taatcatcac gtatatttgg atcatttctg
     5341 tgaatgaaaa tgcatgtaag gacacatgtg cacgggtaat atacgttatg aatggtgtca
     5401 tccaccaaaa agatgaaaca tatacgacga tgaccgcaat gacgaacgac aataaaattt
     5461 ttactaaccg ttggcgtagt tcgctaaagt gaaccattaa tgtatcatca gtgctattgc
     5521 tctcgctgtt gcttcgattc cttactgggt gtatcgtgag actctttatc taaatcttct
     5581 gtagcagatt taaattcttt taaagtagaa ccgatagcac gaccaaattg tggtaatttt
     5641 ttcggaccaa aaataattaa agcgataatg ctaatgacga caagacttgt tggacctgtg
     5701 atgcctaaaa taaaagtgtt agttatcatg ataatcaacc tcactcataa gtagtatata
     5761 gtatttactt tataccgaac gataatgatt atcaacaaat tttatataat aattaaaata
     5821 ttgctataaa cacaacttac tgaggtatta attctgaaat aatagcttgt tcttgcaagt
     5881 cgatataagt acaagtacca gcttgaatat cgatatgcca tttataaatg ccagcttgag
     5941 ccatttcatc acaaaatgtt tcgaaatctg tttgtccttg ttgatgtcta gttaatacat
     6001 cttgaacgat agattgattt gaacttggag cgacaggatg attacttttt acagatgacg
     6061 taacgatatc atcttctgat tgatgtacgt atgttgctgt gccatcttga atattgacga
     6121 tattgtaagt catccccata tctttaaaag ccttgaatag ttttggaaaa tcaacaccag
     6181 taaattgttg atgtgcttgt tgaattgcag ataatttaaa tgccataata atctcctttg
     6241 aatggtttga tttagtgaaa atgcgtgcat catactaaaa ttagaatgct gttaattatg
     6301 caggaaggtg taacgaaagt aaacaattat gacttgtagg tgttgaggtg tgtgtttcaa
     6361 aatgtcatac ataggtggac acttatattt tttgtgtaga tttgacaaat atagttgtca
     6421 aaaagacaat tatcatttac agtgtagttg aggagttgat gcgatgtgcg taatcgattg
     6481 aaagaattac gagcacgaga tggcttaaac caaacgcagc ttgctaaaca agcgggtgtt
     6541 tcaagacaaa ctatttcgct aattgagcga aacaacttta tgccatcggt attaactgca
     6601 ataaagattg ctcgtatctt caatgaaaag gtggaaacta tttttattat tgaggaggat
     6661 gaggtttgaa aatactaaga tatatcggat atcttttact aggtggactt gtaggtggta
     6721 tcataggtgg aattttaggt aactttgatg gattgggtat tgagaatttg acgttcgcaa
     6781 cgcataacaa tgtcgttgta atatcaatta ttgcgactat aatcatcata ttaatagaaa
     6841 tcattgtttt gatgaatcaa agacgtgcat tgaagtataa gcgacttgta gatgaagagg
     6901 tagataacga agaaacagat caatatgaat tgcttgcgaa tcgtcatgtt ttaaatggaa
     6961 gtatattaag tattctacag acagttattg ccttgttagt gttgcttatt tttgtggtag
     7021 gacaagctga agtaaatgga atactattat ttttaatacc attttttgct agtgctattt
     7081 tcaatacaca atttacgctg tttaatagaa gatttgatga tagaatgcca aaaattgcag
     7141 ataagaatta cactgaaaag cgattggaaa tattggatga aggtgaacac catatagaat
     7201 taattgcatt atttaaaaca tatgcaatca acttatccat attgatacta gccattattt
     7261 ttatagggtc ttattcaatt gctacaggaa ttaatcaaag ctttagtttg ctacttatca
     7321 ttgctatttt catatataac gcctttagtt atttattgaa gagaagacgt ttttattaaa
     7381 attaaacaaa gaggagcatg gatatgacaa cattgttaaa cttagatcgt gtgaacaaac
     7441 aatacaagtc ttcggatttt aaattacaag atgcatcttt aacgatttct accaatgaga
     7501 cagttggatt aattgggaaa aatggctcag gtaaatcgac attaattaat attctagtag
     7561 gcaatcgaca taaagataac ggtagtatca cattttttgg ggaagaacat gctattgatg
     7621 atgtcgaata taaagaacac ataggtgtag tgtttgatga tttgagagta cctaataaat
     7681 tgactattaa agatattgat aaagtatttc aatctattta tatgacttgg aatagtcaaa
     7741 aattctttga tttaatcaaa tatttcgagt tgccactaca aactaaaatt aaaacttttt
     7801 caagagggat gcgaatgaag atagctttga cgattgcgct ttctcatgat gtgaagctat
     7861 tgatcttaga tgaagcaact gcgggtatgg atgtttctgg acgagaagaa gtaatggaac
     7921 tattagaaga ttttgtcgcg caaggtggag gcatcttaat atcatctcat atttctgaag
     7981 atatagaaca attagcggat aaattagtgt ttatgaaaga tggacgaatg attttaactg
     8041 aacagaaaga tatactttta gcacaatatg gaattgttac gacagaagat aaagatgttg
     8101 aaattcctaa gcatttaatc attgcttcta gattatcgaa ggggaaatat caaattttag
     8161 ttaaagatta tgcagaaatt gaaaatgcag aacctttaaa acacattgat gacgctacga
     8221 aaatcataat gcgaggtgaa gtataatgaa aggtatgttc ctaagtagtt tttatgcaac
     8281 gagaaagcaa acatatattt attttatagt cgctatcata gctgcgggat actttgcagt
     8341 atttaatccg ttgatgagtt cggcaatggc tggggttatg ttaattacac ctattactga
     8401 taatattaaa catgaaaaag actcaagatg gatgtattat gtatctactt taccggttaa
     8461 acgtagtgat tatattaagt catactttgc cttttattta atcttattcg gtgcaagttt
     8521 aatgattgga ttagttgtga ctacgattgt gacccaaagt gtgatgattg gtattatgtc
     8581 aggtttaatg agttttggta tcataggggc atactctatc attttcccat tgacatttaa
     8641 atttggcgct gaaaactcca atgtcattat gatatgtgca tctatactac tacttatttc
     8701 tttcgtggtc tttttcttta tatatggtat ggttagtggt gcatctgcat tagaatttga
     8761 aaaaattagc actggaggat ggctagttgt catagtatat gcggttatcg gtatagttat
     8821 aacaagtgtt tcttatatat cgtctattaa aatttttaac aaacaagaac tataatcaat
     8881 attcttggtg tgaagctagt cattgaaata atatttagag caggtgataa atctttacga
     8941 ttgtcatctg ttctttttgg tgtggaatga aatgcggggt gataagtata ggtgacatat
     9001 ctagattgat ttatttgttt tgaggtggtt atgttgtgag ggaattattt ccttttagat
     9061 agtgggggtt agagaatata tgttatttat gagtatcatt tgatgattgt ataggctaac
     9121 gatttcctcg gaaatattta aaaacctcga tcatgtatca taactgaagt ttgtcacaaa
     9181 gtataatgtg aagttcgaca cttttggatt cagttcaaat actttgaccg aggtaaatac
     9241 tatttattca tgtttattac gtagacgttg atttttaaaa taaatcagca tgatacagaa
     9301 ggttgcaact gatactgaga taatcattac atggtcgtga cctttaaata aaaggctgac
     9361 aatataagac ataacgagta tccctagtga atatgaaata tacttcgcat ttgtcaattc
     9421 gttatggaaa taaggcgtga ttaaccataa tccaatatag aatattaaaa cactgatata
     9481 catcatatta atttcaaaca agtcatttaa tttattgtta ttactaaaaa caattgcggc
     9541 attaatcaca cctaaagcga tattgattaa taaatgcgta tacgataaac ggaaaccgat
     9601 agatgacaat ttatgattaa tgtaattttc agtaatgatc caatatacac cgaaaagact
     9661 aattaaaatc ataaattgga atatataaat gtaactaaaa tgatcaatgc taaatgatga
     9721 cgaagctaaa ccaaccagta cctcgccaaa gatgataatt gtcagtaatg aaaaacgttc
     9781 tactaaatgc atcatattaa caggtgataa tacgagatat ttttgaaatg gaataagtcc
     9841 tgtcgatgca atgaatacgc ctaaaaatcc agggatgtaa tggatacttt gtggtagtac
     9901 taatgataga aatgataaaa atgaaatcac aaaggctacg gtcgcaaaag cttgacatgt
     9961 acgcttatcg ccataatcta atcctgtacg tatatgtaat aaatactgta atccgatact
    10021 taaatacata attgccacgc ataagaagaa tgggaagaat gtcttttcaa agtccggata
    10081 taggctgtta gataggaaga ccatgataaa catattaaac atcataaacg agacgtcttt
    10141 gaatgtaact tgaccaaatc gatttgtaaa aaatgtttga tgagaccaca ttaaccataa
    10201 gaacaaactc atgacgatgt atttgaaaaa taaatcagct gaaatggaac cattttgtgt
    10261 tgttaaaatc acatgtgcaa ttttttgaat ggcatagacg aaaattaaat caaagaacaa
    10321 ctcatggaat cctgcacgct tttcagctaa atgttttggt gttaatgcat taaccataaa
    10381 attttaactc ctttaaggtg tgtaattaat ttactaagta tactatttat tttttctagt
    10441 gaataggggc agatttgacg atgaaatgga aggaggggtg acagggagct ggttgcggca
    10501 ttaacagtta tcagcgattt aatatttgaa tagagacgtc atggtttgaa aaagtagatg
    10561 gtgaaattga tggtgaaaca agctgtgaat agcgatgcaa tgatagatag catttaatta
    10621 gagtcattac gcgaaatgat taatgataat ttgtggtaaa tcaaagcata attttgtact
    10681 atagatgagg atgatagagc atatttaaga gtgtgaaatg ttaaagtgaa accgtttacg
    10741 tttccgattg cccaaacaaa ttacatcatt gtataatatg atttgttaaa tgcataacaa
    10801 gaatgaaaat gtaacatacg tagcaattgg tttcataaat tggatgttag tggcgtattg
    10861 gttcattaga cgtattagta ataaaattgt atatatcata aggagatgaa tatgacatga
    10921 cgagagtcgt attagcagca gcatacagga cacctattgg cgtttttgga ggtgcgttta
    10981 aagacgtgcc agcctatgat ttaggtgcga ctttaataga acatattatt aaagagacgg
    11041 gtttgaatcc aagtgagatt aatgaagtca tcatcggtaa cgtactacaa gcaggacaag
    11101 gacaaaatcc agcacgaatt gctgctatga aaggtggctt gccagaaaca gtacctgcat
    11161 ttacagtgaa taaagtatgt ggttctgggt taaagtcgat tcaattagca tatcaatcta
    11221 ttgtgactgg tgaaaatgac atcgtgctag ctggcggtat ggagaatatg tctcaatcac
    11281 caatgcttgt caacaacagt cgctttggtt ttaaaatggg acatcaatca atggttgata
    11341 gcatggtata tgatggttta acagatgtat ttaatcaata tcatatgggt attactgctg
    11401 aaaatttagt agagcaatat ggtatttcaa gagaagaaca agatacattt gctgtaaact
    11461 cacaacaaaa agcagtacgt gcacagcaaa atggtgaatt tgatagtgaa atagttccag
    11521 tatcgattcc tcaacgtaaa ggtgaaccaa tcgttgtcac taaggatgaa ggtgtacgtg
    11581 aaaatgtatc agttgaaaaa ttaagtcgct taagaccagc tttcaaaaaa gacggtacag
    11641 ttacagcagg taatgcatca ggaatcaatg atggtgctgc gatgatgtta gtcatgtcag
    11701 aagacaaagc taaagaatta aatatcgaac cattggcagt gcttgatggc tttggaagtc
    11761 atggtgtaga tcctgctatt atgggtattg ccccagttga cgctgttgaa aaggctttga
    11821 aacgtagtaa aaaagaatta agtgatattg atgtatttga attaaatgaa gcatttgcag
    11881 cacaatcatt agctgttgat cgtgaattaa aattacctcc tgaaaaggtg aatgttaaag
    11941 gtggcgctat tgcattagga catcctattg gtgcatctgg tgctagagtt ttagtgacat
    12001 tattgcatca actgaatgat gaagttgaaa ctggtttaac atcattgtgt attggtggcg
    12061 gtcaagctat cgctgcagtt gtatcaaagt ataaataata agaaaacagg ttatcacaac
    12121 agtattaatt acatgttggc ataacctgtt tttatttgtt tatggattta ttgggtaata
    12181 ttagtcattt gatggtttaa ttgcaaatgc tctaacaggg aacccaggtg catcttttgg
    12241 tttagggctg atagcgtaaa tgatggcgcc acgagttggt aattgatcta aattagttaa
    12301 taactcgact tggtatttat cctgaccaag aatataacgt tcgccaacta aatcaccatt
    12361 ttttacaacg tccacagatg catcggtatc gaatgtttca tgaccaacag cttcaacacg
    12421 gcgttcttca attaagcact tcaaagcatc taatccccaa cccggtgcat gttgttgtcc
    12481 gttcgcatct ttgttttcaa acgtttcaat attaggccaa cgttttgacc aatcggtacg
    12541 aagtgcaaca aaagtgccag gttcaatagt accatgctct ttttcccatg cttctatatg
    12601 cgcacgtgtt acgatgaaat cattgttgtt cgctacttct gttgaaaagt ctaatacaat
    12661 taacggcaat accaattctt ttaaatcaat gtcttctaaa taacgtttat tctcgacaaa
    12721 gtgaattggt gcatcaatgt gagtaccata ttgcgttaca atattccaac gttgcacata
    12781 gaaaccatga tctttaaccg tgaataaagt tgaaacttcg cctttttcaa actcactaaa
    12841 acgtggtatt tccggatcaa acgtatgcgt taaatcaacc caagttgctt gttttaaagt
    12901 atttaattgt tgccataaag gatattgtgt cataaaatca cccgttttta gtttattata
    12961 tgataaatgc tgcgattatt cttggcgttt agctttaaca gcattcacaa gcacagtcaa
    13021 tgcatcttta acttcttctt cttttcgcgt tttcaaacca cagtcagggt ttacccagaa
    13081 taatgagcgg tcgatttgtt gtagtgaacg attgattgct gtagtaattt cttcttttgt
    13141 tggaatacgt ggactatgaa tatcatatac acctagacca atacctaaat cataattaat
    13201 atcttcaaag tctttaatta aatcaccatg gctacgagat gtttcaattg aaataacatc
    13261 agcatctaag tcatgaatag catgaatgat ttgaccgaat tgagaataac acatatgtgt
    13321 atggatttga gtttcatcac gaactgaaga cgttgcaagt ttaaatgata aaacagcatc
    13381 tttaagatat tgttcgtgat attcagagcg taatggtaag ccttcacgta atgcaggttc
    13441 gtcaacttgg ataactttga ttcctgcagc ttcaagtgct aatacttctt cgttgattgc
    13501 taaagcaatt tgatcttgaa caactttacg tggtaaatca acacgttcaa atgaccagtt
    13561 tagaattgtt acaggtccag ttaacatacc tttaactggt ttatctgtta agctttgtgc
    13621 ataaactgtt tcatcaacag ttaaaggcgc tgtccatttt acatcaccat aaatgattgg
    13681 tggttttacg gcacgtgaac catatgattg cacccaaccg aatttagtta ctaggaaacc
    13741 ttgtaatttt tctccgaaga attcaaccat gtcattacgt tcaaattcac cgtgaactaa
    13801 tacatctaag ccgatgtctt cttgaatttt aatccatcga gcaatttcat tttttaagaa
    13861 tgtttcatat gcttcgtctg taatgcgttt gtttttccaa tctgcacggt attttcgaac
    13921 ttctcggctt tgtgggaatg atccaatagt tgttgttggt aaatccggta agttcaaacg
    13981 tttttgttgt tgttcaatac gttgcgcgaa tggtgattgt cttgaagtac gcacgctttc
    14041 gaaatcataa tctaagtttt tgaatgattg attttggaaa cgctcataac gtgcttttaa
    14101 tttatcatat ttaacactat cgttttgatt aaataggcga cgcaatgcat ctaattcgtc
    14161 taatttttca gttgcaaagc ttaagccttc gccaacactt gtatctaatg tttcatcatc
    14221 taaagatact ggaacatgta ataatgaaga tgatggttga atgacaagtt cattagtgtg
    14281 tgctaacaat ttatcgatta agactttttt agcttcaatg tcacttgccc atacattacg
    14341 accatcaata attccagcat ataatgtttt tgatttatca aaatctccag cttcaatttg
    14401 tttaaggtta tagccattat catggacaaa gtctaatcct aaaccaccaa caggtaaaga
    14461 acttaagaat ttaagatgtg cacgttcgaa gtatgtttga atgactaatt ttttagcaac
    14521 accagctttt tcgaaatagt cataagcttc acgtgtaata ttttcatagc tttcactgtc
    14581 gtctgtaact aagattggct catcaacttg aatgtactca gcacctgcat caattaatga
    14641 ttcaaacact tctttataaa gtggtaataa cgttttaact ttttcttcaa aagtttggtg
    14701 accgcctttt gataatttaa caaaagtaat cggaccaaca ataacagggt gagcattaac
    14761 atttaaagat tgcgcgtatt taaagcgatc taataataca ttgcgactca ctttaggctc
    14821 aacattgtcc cattcaggta cgatgtaatg atagttagtg ttaaaccatt ttataagtgc
    14881 acttgcaaca tggtctttat taccgcgagc tatatcaaat aataagtcat catcgatagt
    14941 tcttccttgg aaacgttctg ggatgatgtt gaataataat gacgtatcta aaatgtggtc
    15001 atataaagag aaatcaccaa ctgggatgct atctaagtga tagtactttt gtaataataa
    15061 attttcttta tgtagatcag ttaatgtttg atctaattct tctttagaaa tcttctttgc
    15121 ccaataactt tcgatggctt ttttccattc tctttttcta cctaatcttg ggaatcctaa
    15181 gtttgatgtt ttaattgttg tcataatatt gcctccttgt gagcagtaat agattttgag
    15241 tatgctgcaa gttctaatga atcttcgaca ttttgaaacg gtgtgataat gtataaacca
    15301 ttaaaatatt catgaacagt atcgattaaa tcctttgaaa gcttaaggct tagttcacga
    15361 gttttggctt tatcatcttt aactgcttca aattgttgta aaatttcatc tgacatcttg
    15421 attcctggca cttcattatg caaaaagagt gcgtttttgt aacttgcgat aggcataatg
    15481 cctatgaaaa atggtttgtt caagtgctta gtggcatggt aaatttcaat gattttctct
    15541 ttgctgtaca cgggttgtgt tataaaataa gacattccgc tttctatctt tttctctagt
    15601 cgttttacgg caccatctaa tttacgaaca ttagggttaa aggcgccagc gatgttgaag
    15661 tgtgtatgtt tcttcagcgc atcaccgtca gtgttaatac cttgattaaa tcttagagcg
    15721 agttcggtta atcctttaga attaacatca tagacattgg ttgcaccagg taggtgacca
    15781 acttttgaag gatcaccagt tatggctaat atttcgttaa cgccaatgag tgataatcca
    15841 agtaaatgag actgcaagcc gattaagttt cggtctcgac atgtaatatg tacgagtggt
    15901 tcaatattgt aatattgttt aattaagctt gcagcagcaa tgttgctaat tctgacagtt
    15961 gccaatgaat tatctgcgag tgttaccgca tctacattag ctttttcaag tttagcgata
    16021 ttttcaaaaa atctatccgt gtctaaatgt ttcggtgtat ccaattcgat aataacggtt
    16081 ggacgttctt gaaccttaga tgttaatgat tgtctaactt tattttgaga tggattgaaa
    16141 agtgctttcg ttggtatcgg aatcactttt ttgtcattaa caggtttaag tgtctgaata
    16201 gattctttaa taaatttgat gtgctcaggc gttgtaccac agcaaccacc aattaaacga
    16261 acaccttcgc gaattagatt ttgagcaact tgaccgaaat attgtgcatt gtcactatac
    16321 ttaaattcac tattttcaat atctaataag ctggcatttg gataacaaga taagtatgcg
    16381 tgctctggta attcaatatg tgtgaaagac tcttgcatat ggtgtgggcc atgatgacaa
    16441 ttgagtccca cgatgtttgc accacattga acgagttgtt ttaatccttc attgattgcc
    16501 tgaccattaa ctaagtaatt tgtgtttgaa gcggttaatt gagcaatgat tggaatgtcg
    16561 tatttctttc tcgttcgtga aatgacattt gttagctctt ctaggtcgta atacgtttcg
    16621 aaaagtagcg cgtcaacacc ttcttcaatc aaggtgtcta tttgaatttc agtatgataa
    16681 agaatagttt gtaagctgat atcctcttgt ttgatacctc taaacccacc aactgtgcct
    16741 aatatatacg tatctttatt tgctgctttt tttgcgatgc gaacggctgc ttgatgtata
    16801 gccttaactt tatcttcaag accgaatcgt tttaactttt caaaatttgc accataagta
    16861 ttggtttgaa tgacatcagc accggcttca atatatgaac gatggatacg ttcaacttta
    16921 tctggatggc taaggttata tgcttctggg caggtgtcta aaccttcaga gtataaaata
    16981 gttccaatag cgccatcagc tactaaaaca ttatctttca agtgtgtgag gaattgactc
    17041 attgaatgcc tcctttaatg cgtatttgat gtctgcaatg agttcatcag gatcttcgag
    17101 accaacactt aatcggaaca gaccgaaagt gataccacgt tcttgtctca cttcttcagg
    17161 tagtgcagcg tgagacattg ttgctggatg tgaaagtatc gtttcaacac cgcccagact
    17221 cactgaaacg agtggtaatg tcagtgcatc gacaaattgt tgtgctttag actcatcagc
    17281 taaacgaaag ccaataacgg caccgccatt tttagcttgt tctaaatgag tagcagtaag
    17341 tcccggataa taaacttctg aaatttcatc ttgctttatt aaaaatgaca cgattttttg
    17401 cgcgttttcg acagattgtt taaatctgat tggaaatgtt ttcaaatgtt tagcaagcgt
    17461 ccagctatct tgagcagata acatattgcc cgtaccattt tgtattaaat aaagtgcttc
    17521 actcattgct tcattattag taatgacagc accagcaatt aaatcgctat gtccacttaa
    17581 aaattttgta gcactatgaa tgacgatatc agcgccaagt aataaaggtg attgacctaa
    17641 cggtgtcata aatgtattgt ccacagctac cagtagttca tgcttttcgg ctattttaga
    17701 aacagctttg atatcagtaa ttttaaaaca gggattcgat ggtgtttcga tataaattaa
    17761 ttttgtgttt gattgaatgg caccctcgat ttgttcgagc ttggtagtat ctacggttgt
    17821 aaattcaata ttaaatcgat tcaaaatttg ctcagtgagg cgaaaagtac cgccatatac
    17881 atcatcgggt aagatgacat gatcaccaga tttgaaagtc aaaagtactg ctgaaatagc
    17941 agcaatacct gatgcaaaag caaaagcgaa tttcccctgt tctaatcgtg ctaacttctc
    18001 ttctaaaagt tcacggttag ggttgccact tcgtgcataa tcatatttaa catcgccacc
    18061 aagacttgtt tgatggaatg ttgaagaatc atagagtggt gggttagctg aatgatattc
    18121 cacacctcta cgccaatcga atatcacttc tgtctctttt gaaagtgtca tacaatctct
    18181 ccaatctgag ctttatctaa tgcttggatg atatcgcgtt cgatgtcttc ataattttca
    18241 acacctagtg ataagcggat taaatactca tcaatgccac gtttatcttt ttcagcatct
    18301 ggcatatcaa catgtgtttg ggtgtaaggg aaggtcacta atgtttcagt acctcctaaa
    18361 ctttctgcaa aaattcaaat gtctaaattt tctaataatt tagcgacgct ataggccttg
    18421 ttaagtctta aactaagcat gccagtttgc ccgctatata gtacttcgtc aattgcttga
    18481 agtgactgac atttttcagc aagttttctt gcgtttgatt gcgcacgctc aatgcgtaaa
    18541 tgcaaagttt taagtccacg taacaacaaa taactatcta ttggtgatag tgttgcgcca
    18601 gtcatgttgt gaaaatcaaa caacttttgc gcgagtgatt catctttgac ggttacgaca
    18661 cctgctagta catcgttatg tccaccaata tatttcgtag ctgaatgtaa gactatatca
    18721 gcaccttctg ctaatggtgt tgaaagataa ggtgttaaaa aagtattgtc gataattgac
    18781 aataagcctt tagctttaca aagttgatag tacggcttta catcaatagc aatcatttgt
    18841 ggattggata ttggttcaat gaataatgct actgttttat cagtgatttc tttttcaact
    18901 tgttcataat ctgtaaaatc aacgtactta aatttgatat cgtattgttg ctcgtaaaat
    18961 tcaaataatc taaatgtgcc gccgtataaa tcgaatgaaa ctaaaatttc atcgtgtggt
    19021 ttaaataaat tacatattaa ttgaatggct gacattccac ttgatgtagc gaatgatgca
    19081 ataccatgct caagtttggc aaaacaggtt tcaaatgttg agcgtgtagg atttttagta
    19141 cgtgtataat caaatcctgt cgattgtcct agttttggat gcttgtaggc agtagataaa
    19201 tggactggat tcgctatagc accggttgaa tcatcagtta atgtgatttg ggctaactgt
    19261 gtatccttca tattaagacc ctcctagtaa gaaaaaataa aaaagcttcc gtccttcgta
    19321 cccgaatgaa tcggataaaa aggacgaaag cttatgtttc gcggtaccac ctttatttgt
    19381 tattccatcg ctgaaataac cttattcagt acgcattaaa agtaaatatg cttactgaac
    19441 aattatcaca atttaagtcg ttaagtaagg atacagtaat gtgctatccc atacttatta
    19501 acaaaaaatc gtgcgtaaag aatccagtac gccatttaac atcaatgtta atactgtatc
    19561 gctataacgg gcgaacccgt agacacctca tattggcatc aacactccaa ggccattttc
    19621 aaacacgctt tcaaaatctt ctctcagcta ctaaagactc tctgtataag cagggtgtgt
    19681 tttactttcc tctttattgt gtttacgttt cattaaactg ttataagata ttaattagct
    19741 tacagagtaa aaaaagattt gtcaacaatt attcagaaaa ttttgattta aaagttaatt
    19801 tgtttgtgaa attgtaattg gtatcttgaa gttgaaaaat gaattatttt ttaaataaag
    19861 tgtggttaat ggttgtctga ctcatttaga atacataaaa tatatttaac tgttgttatc
    19921 aaataaaaag tgatgtgagt gaattgtcaa aaagtgaaga tcaacgtatt actaaaacaa
    19981 aagatgaaca aattaagcaa atagatatat cggatatcaa accgaatccg tatcagcccc
    20041 gaaaaacttt cgatgaaaat catttaaatg atttggcaga ttcaattaag caatatggta
    20101 ttttgcaacc aattgtgctt agaaaaacag ttcaaggtta ttacattgta gttggtgaaa
    20161 gaaggtttag agcttcgaaa attgctggtc taaaatacgt atcagcgatt atcaaagatt
    20221 taacagatga agatatgatg gaactggcgg tcatcgaaaa tttacaacga gaagacttaa
    20281 atgcgattga agaagctgaa agttatcaac gtttgatgac agatttgaaa attacgcaac
    20341 aagaagtagc gaagcgattg agtaagtcgc gcccgtatat agcgaatatg ttgaggttat
    20401 tacatttacc gaaaaagatt gctgacatgg taaaagatgg gcgactgaca agtgcacatg
    20461 gacgaacgtt attggcaatt aaagatgaac aacaaatgct taggttagcg aaacgggttg
    20521 ttaaagaaaa gtggagtgtt agatatttag aaaaccatgt taatgaatta aaaaatgttt
    20581 cgtcaaagtc ggaaacagac aaagtagata taactaagcc taaatttata aagcaacaag
    20641 aacgacagtt gcgagaacag tatggcgcaa aagtagatat atcgataaaa aaatcagtcg
    20701 gcaaaatctc atttgagttt gaatcgcaag atgactttgt gagaatcatt aaacaattaa
    20761 atcgtaagta tggtaaatag ttacacaatt ttatatagta tcactttgtg aatctgtaga
    20821 aatatcgata gttatctata ttctttgaag atgatgcgac ttcagtaggt tggaagcata
    20881 caataatatt aataaaatta tttgagacta tatagaaggg agtaacgcta attatggatc
    20941 aagttatgag tattatttca tcgctattcg aaccattaac taaaattgaa acatatgaaa
    21001 gtgtcgctac taaattagcg atgataatta tatatattat cgttgcatta atcgtaatta
    21061 aaatacttga taaaatgatt gcccaaggat ttaaaataca aaacaagagt aaaaagggta
    21121 ataaaaaacg ttcgaaaaca ttaatatcat tggtgcaaaa tgttgttaaa tatattgtgt
    21181 ggtttatagt tgtaactacc attttaagta aatttggcat tagtgttgaa ggagtaattg
    21241 caagtgctgg tgtcgtaggc ctagcagtag gttttggtgc tcaaactata gttaaagacg
    21301 taattacagg attctttatt atatttgaaa accagtttga tgtaggtgat tatgttaaga
    21361 taaacaacgg tggtacaact gtagcagagg gaacagttaa atcaattgga ctccgttcaa
    21421 cacgcattaa tacaatatct ggggaactta caatcttgcc aaatagtagt atgggtgaaa
    21481 taacgaatta ctcaattaca aatggaacag caattgttaa aattccagta tctgtagatg
    21541 aaaatattga taatgttgag aagaaattaa acaaattatt tacatctctg cgtagtaaat
    21601 attatttatt tgtaagtgat cctgttgtga ttggtattga tgcctttgaa gatactagag
    21661 taatattaag ggtttcagca gaaactatac caggtgaagg tttctctggt gcgcgtatta
    21721 ttcgtaaaga agtacaaaaa atgtttttac aagaaggtat taagacacca caaccaataa
    21781 tgacaccttt taatcatagt gaaaacggtg tttagtagtt tataatacat ggaggtcata
    21841 tttaatggcg tcaaaatatg gaataaatga tatagtagaa atgaaaaaac aacatgcgtg
    21901 tggaaccaat cgttttaaaa ttattagaat gggtgctgac attagaatta agtgtgaaaa
    21961 ttgtcaacga agcattatga ttccacgtca aacgtttgat aaaaaactaa aaaaaatcat
    22021 cgtatctcag gatgatacac aaagatagga gaatgattca tggctttaac agcaggtatc
    22081 gttggcttgc caaacgttgg taaatcaaca ttatttaatg caattacaaa ggcgggtgcc
    22141 ttggcagcaa actatccgtt cgcaactata gacccaaacg tgggtattgt agaagtgcca
    22201 gatgcaagac tacttaaatt agaagaaatg gttcaaccta aaaaaacatt acctactaca
    22261 tttgaattta cagatattgc cggaattgtt aaaggtgctt ctaaaggtga aggtttaggt
    22321 aataaattct tatcacatat tagagaagta gatgcaattt gtcaggttgt tcgtgctttt
    22381 gacgatgata atgtaacaca tgtagctggt cgagttgatc caattgatga catcgaagtt
    22441 atcaatatgg aattagtact tgcagactta gaatcagttg aaaaacgctt acctagaatt
    22501 gaaaaattgg cacgtcaaaa agataagact gcggaaatgg aagtgcgtat tttaacaact
    22561 attaaagaag ctttagaaaa tggtaaaccc gctcgtagta ttgactttaa tgaagaagat
    22621 caaaaatggg tgaatcaagc gcaattactg acttctaaaa aaatgcttta tatcgctaat
    22681 gttggtgaag atgaaattgg tgatgatgat aatgataaag taaaagcgat tcgtgaatat
    22741 gcagcgcaag aagactctga agtgattgtt attagtgcaa aaattgaaga agaaattgct
    22801 acattagatg atgaagataa agaaatgttc ttagaagatt taggtatcga agaaccagga
    22861 ttagatcgat taattagaac aacttatgaa ttattaggat tatcaacata ttttactgct
    22921 ggtgtgcaag aagtacgtgc ttggacattt aaacaaggta tgactgcacc tcaatgtgct
    22981 ggtatcattc atactgattt tgaacgtgga tttatccgtg ccgaagtaac aagttatgac
    23041 gactatgtac aatatggtgg cgaaagtggc gctaaagaag cgggcagaca acgattagaa
    23101 ggtaaagaat atattatgca agatggcgat atcgttcatt tcagatttaa tgtataaacg
    23161 atagagtgaa gttaattaaa tagtatatat gtagaagagg cggaatcaat tgttcgcctc
    23221 ttttaattat gcgtataatt tattaaaaga atggaataat tttactcgcg ttaataatat
    23281 cttgagtgct gaaaaattgt ttgccttcgc cagtataagc aggctctaaa acaagattag
    23341 cctttgcaca ataaagccat tcaggatgaa tgccactatt aagtatctct tggaattctt
    23401 gaaaatcttt agaccaatca atatttaaat tcattcctta ccacctcaca atcattatag
    23461 aacatgtgtt cgcttttgtg aagtgtattt ttaaaaatat catagaaaat ttcaaatgaa
    23521 ttaatgtcaa aaatgatatg aagagtagtt tgaattatta ttgtaaaaag taatggcgca
    23581 tgatataatt ctttattgtg agtaatgaaa attattcctt gcttatctgt tttaagattg
    23641 ataagccgta tagaccacaa ggaggtgcaa atataaaatg agaacatatg aagttatgta
    23701 catcgtacgc ccaaacattg aggaagatgc taaaaaagcg ttagttgaac gtttcaacgg
    23761 tatcttagct actgaaggtg cagaagtttt agaagcaaaa gactggggta aacgtcgcct
    23821 agcttatgaa atcaatgatt tcaaagatgg cttctacaac atcgtacgtg ttaaatctga
    23881 taacaacaaa gctactgacg aattccaacg tctagctaaa atcagtgacg atatcattcg
    23941 ttacatggtt attcgtgaag acgaagacaa gtaataatta gagggggcgt ttaaatgcta
    24001 aatagagttg tattagtagg tcgtttaacg aaagatccgg aatacagaac cactccctca
    24061 ggtgtgagtg tagcgacatt cactcttgca gtaaatcgta cgttcacgaa tgctcaaggg
    24121 gagcgcgaag cagattttat taactgtgtt gtttttagaa gacaagcaga taatgtaaat
    24181 aactatttat ctaaaggtag tttagctggt gtagatggtc gcttacaatc ccgtaattat
    24241 gaaaatcaag aaggtcgtcg tgtgtttgtt actgaagttg tgtgtgatag cgttcaattc
    24301 cttgaaccta aaaatgcaca acaaaatggt ggccaacgtc aacaaaatga attccaagat
    24361 tacggtcaag gattcggtgg tcaacaatca ggacaaaaca attcgtacaa taattcatca
    24421 aacacgaaac aatctgataa tccatttgca aatgcaaacg gaccgattga tataagtgat
    24481 gatgacttac cattctaata aaaattaacg aaattaaagc gaaaaaatta tcaaaggagg
    24541 cacacaatca tggcaggtgg accaagaaga ggcggacgtc gtcgtaaaaa agtatgctat
    24601 ttcacagcaa atggtattac acatatcgac tacaaagaca ctgaattatt aaaacgtttt
    24661 atctcagaac gcggtaaaat tttaccacgt cgtgtaactg gtacttcagc taaatatcaa
    24721 cgtatgttga ctacagctat caaacgttct cgtcatatgg cattattacc atatgttaaa
    24781 gaagaacaat aatatataac gtataacaaa accctttagc aatcaggcta gagggttgtt
    24841 tttgtgttta tttttataaa aatttcgcaa ttttttcgca gagtgtgaaa agtttgatga
    24901 ttaaaattaa aaaaacggac aaataattgt ccgtctttgc gagatattaa tctcatataa
    24961 tagtttggtg aggactcgcc tctatatatg taaatgataa ctgattatta tttgtacgtc
    25021 aatctaattg attagcttat ttttaatttc attatctact aatttcatac tatcaaaatc
    25081 gagtttcatt ctaccagaag gatcttcagc gttaattttt aaaattctta atttgctaat
    25141 agttactaca ttctgtgtgc aggcaaatgt atttttatca aattttttat atttattaat
    25201 aacttctcta aggttattaa cttttgattt tctatcgttt acatcaatta gcaaataatt
    25261 aaacaaagca gaaaaatttt tcgggtcatt gtataaatta aatgattcta ctatgttttt
    25321 acttttatta attacttctc tttctgtagg gaagcgtgtt ttaattgcaa aatcatctaa
    25381 ataatctata gtgttttgtt ccaaagtgtt ttgtaaatta tttttattaa taatttctat
    25441 aaccgtaaaa attacgtgat ttataacgtc taaatcaaat tcgatattat caactgtctt
    25501 gttcaaaatt gataatgatg ttttaaatac aatattttgt aatggtaaat agtgtttttt
    25561 attttttgaa cttaatggta caactgttac taaagaatta tatggactat catttttgtt
    25621 taatactatt ccaaaatgtc tgccagaaaa ttcatgccca atattgatgc caaaatctaa
    25681 atatataata gatccccttg agaatttttt gtattttttg tttatattgt tattatgctc
    25741 catattaaac caatatgatg ttgtctctaa ccaatttggt aaaaatttaa actttttatt
    25801 atctgtgtta tataacagaa taaatactca cagctttatt tattctgtta tccatctaac
    25861 tccctatccc ctctaattta ttcatcatat cttttgccat cttatcagta acatgtgtgt
    25921 atatctctaa ggtggtttta tagtccgagt gacctacacg ctcttgtatc gcttttaggt
    25981 taattcctaa ttgcgcaagt gtagatatgt gtgtgtgacg taatgtgtgc gttgtcacac
    26041 gcttgttaat tgaacttata tcagtagctt ctttaataat attattcacc ttatttaagt
    26101 caatagggct accagcagtg ttagtaaata tataacctct atctatgaat ttatcattcc
    26161 actgattctc ttttttattt tctagcatga gttttttaag taaattaata ctttgagctg
    26221 tgaggcctat tgttcgataa ctcttactcg tcttagtcgt ttctttcact ccaaatgctc
    26281 cagttactac atctgtaacc cagttaattg tgccatcaat ctctagtgtt ttattctcca
    26341 cgtctacatt gtctgtcttg attgctagga gttcgccaat gcgcattcca ttgttaattt
    26401 gaaattctac taatgctttt accatttcat agttacgttt acgcgtagca tgacgcttat
    26461 gtttaattag atagtcgaag cactctagta actcctttac ttcgctatct tctaaatagt
    26521 tattacgttt agcttgaaac tcgtttctgg tttgggcttt cttaggtata tctattttat
    26581 ctaacacact aatatcgtgc agatcataat atttaaacgc atatttgaaa acggaacgaa
    26641 taacaataac aagagattga acatggccaa tactatgtga tttagcccat tcattaatga
    26701 tgttttgtaa gtaggtgtgc gtaatcttgc tgatgagtac tttgctatca atagcatttt
    26761 taactgtatt agtattactt ttcttttctt taatagtggt tggtttcgag cctgaatgtg
    26821 tcttgtaatg ctctaaccat tcatcgcacg catcatggaa cgttaagttt tcaagttgtt
    26881 tcgtactgta atgtttcaaa cgttgctcaa ttattttatt taattctaat tgagcgtcct
    26941 tttggctacg tacattattc ttgttacgtg taactgatac tgttttatac ttgccagtta
    27001 aagggtctgt atagcgctct aaatagcgat aggccgtact attgtttttg gtgatttcac
    27061 gaacccacat ttgtcatccc tcctttagat attctgtttt taaaatgtct gaaataaaca
    27121 agtaactatc aagtgtagta caaaataggc aaagttacgt atgtttatag gattgtacgg
    27181 tagtaatatt tgtagtatta gctttaagta tttattttag cgtgctttgt tcagttcttt
    27241 ttatctaact tatcatctat ataatcgctt aattttacta attgttccca gttttcattt
    27301 ttatctctta aagtttttgt gttctctttt ataataactg atttattttc gaaataatta
    27361 tttaaaattt tatcgatgtt atttttatcg ttatccgtta atctaatgcc tttgtaaaat
    27421 ttataattat taatatcttg cagatgaaag tgcaaatcat taatgctaat gttaaaaatt
    27481 gtgaaactct tttcgttatt gtcatcaaaa gatatgaatt ctctagaata tggtatatcc
    27541 atcattctat ctataatttc cattttattt gttacattag aaactttgtt taacttaact
    27601 ttatttttcg ttatcttagc tatttcatca acataaaaat tatactcctc attagtgtct
    27661 tttatgttca ttaagtaact ttcgataagt ttattgcttg gaaaagattt aacaccattt
    27721 tcaatgccac ttatatgccc ttgagaatac tgcatttgtt tacttatttc gcttgctgtc
    27781 ttcccttttt gttttcggat agactttaaa aatctgccta attctttttt taattcatca
    27841 tttgattcaa ccattggcga cacctccgta tttttaatga taaaccatgc ataaaaataa
    27901 tacaatatta ttcttgacat tcctaaaaga aggtgcgata ctctatttat gcataaagga
    27961 ataaaaataa atgtatatat tcttaaggtg aggggtgatt ttatgtttat gactgtaaaa
    28021 gaagttgctc aattgttacg tataagtgaa cgccatactt ataaacttct tcaaaaaaac
    28081 gttataccac atactaaaat tggcggaaag atattagtta acaaagaaag gttattagaa
    28141 actttagaaa aaaaggaggt taaataaatg cctagaacaa agttacaaga ttttccatca
    28201 aaagaaaata cagttacaga accggaacaa gttgtagtaa atccgttgtt tgcgaaacct
    28261 aatacactag ctggtatttt tggaatttca tacagttcgg tcaatcgtat tttaaaagag
    28321 tgggaaaaag atcataaagg tattaatgat ttatattatt cactatcatc aacaatgatt
    28381 gttatcagta ttccgcgatt cgaggagtac atgaaggcgc gtcataaaaa atggatgtag
    28441 gaggcaaggc aatgaaaatg tacttaactt atatctgctt agtttcattg ttaacaattt
    28501 tattactagc aatatctaac atgtatgtcg cttttagtgt gtacggcatg atggtaactt
    28561 atggatttaa tttaacagga gagattacaa cgtgcgaaaa caagttatta ttacaaaaac
    28621 agtagttggc tggtacaaca ttaaagatac tcaacataat ttaatgttaa atataccgcc
    28681 aaaagtattt gaacagtact ttcctgatgt tagtaaagat gttcaagttg cgtgtttaga
    28741 aatggattta tcaaaaatta cagaaattaa aaataagaaa aaggtaggta gttaagatgg
    28801 aaatcaaaca aaaatatcaa ttatcaaaag tggttaaaat attagaagta gtattatacg
    28861 aggaagataa gtttcaatcc gataaggact atcattatca ggataaagca ttttatgaat
    28921 atgctttaaa gttagttcat aatggattgt tcaatattct tgctgaatta gattttgaag
    28981 atgaagtatt tttaattctt gatgaagtaa cgatggcgct aagtgatgtc atgaaagaaa
    29041 cacaacacgt ttatcgttat agtgtcatag acgaaaaagg tgaacataaa catacaacag
    29101 atcgcaaagg acacgtgatt ggaatgttag agtgggcatt agattacatt gtgggaaata
    29161 ttgaagtgga ggtattataa atgaattggg aaattaatga tttgtttagc gatttgaaat
    29221 tgttgaaaga tagattcgaa gatttaaagg ataatcatgg ttggcatttt gaggagttat
    29281 atccacatga accaaatcat aacttaaata aagatgaatt aattagagag ggtgcttctt
    29341 atcatgagag acgtattcac aataatcaaa tgtttgattt attccatctc tatatagagc
    29401 agttcgataa tattatcgaa aagttttatg aaatagaaaa agcatcatct gagaactttg
    29461 gcgaggaatc agatgacgca aagaattcaa taaaagtagc agagtaatat agaaattaca
    29521 cattcttatt ataacatctt tgctctgttg tttcattaag aggtgcaaaa aatgaatgaa
    29581 attaaattag aatatgacac acatgtttca gtggtacatt atgaaagttt agactcacgt
    29641 tcatttaaga gcttttcaaa acctaaatgg agtaagttgg ttaataaact gtctgtacct
    29701 atagaagcga attataagta tgcacgtggt gttgctgttt acggtgatat taaaaacggt
    29761 gcaaatgatc atggtgaaat tatcaaaaag catcgaaacg ataaaaatgt catatacaga
    29821 gatgtgattg tacttgatta tgatgaaata aatga
//
LOCUS       DAMZPP010000011        29698 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMZPP010000011 DAMZPP010000000
VERSION     DAMZPP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 29698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29698
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.011"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(167..910)
                     /locus_tag="POH73_000403"
     CDS             complement(167..910)
                     /locus_tag="POH73_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI6886382.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKFDKKLLIELKSPNLYPGIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRKYWYKKPNFSRIAQIASYVNPNYALVTRKFVDEAHHHQLQVMPY
                     TVNKLKTGEKLRQIGVDGLITDDPKLFIK"
     gene            990..1436
                     /locus_tag="POH73_000404"
     CDS             990..1436
                     /locus_tag="POH73_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDI6886383.1"
                     /translation="MHQHDFKVKTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPHI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            1551..2711
                     /locus_tag="POH73_000405"
     CDS             1551..2711
                     /locus_tag="POH73_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDI6886384.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINYLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKERFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            2698..4302
                     /locus_tag="POH73_000406"
     CDS             2698..4302
                     /locus_tag="POH73_000406"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDI6886385.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHSLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAD
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            4396..4491
                     /locus_tag="POH73_000407"
     CDS             4396..4491
                     /locus_tag="POH73_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886386.1"
                     /translation="MKPLVQSFTSSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(4452..5582)
                     /locus_tag="POH73_000408"
     CDS             complement(4452..5582)
                     /locus_tag="POH73_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6886387.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYRDEPVELKLQNISTNIADRFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDANLFSLKGALWTLAQEVYQEWYLGSKLYEEVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALNGNKRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLSGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(5688..7154)
                     /locus_tag="POH73_000409"
     CDS             complement(5688..7154)
                     /locus_tag="POH73_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDI6886388.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHAAPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAAALGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFVYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIVATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHNHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDQ
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            complement(7282..7899)
                     /locus_tag="POH73_000410"
     CDS             complement(7282..7899)
                     /locus_tag="POH73_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDI6886389.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLITGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            8071..9345
                     /locus_tag="POH73_000411"
     CDS             8071..9345
                     /locus_tag="POH73_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDI6886390.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDSEDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            complement(9439..10701)
                     /locus_tag="POH73_000412"
     CDS             complement(9439..10701)
                     /locus_tag="POH73_000412"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDI6886391.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            11082..11987
                     /locus_tag="POH73_000413"
     CDS             11082..11987
                     /locus_tag="POH73_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDI6886392.1"
                     /translation="MTNQDNNHQLNHRIYQFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTNQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GVPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITKKEYNDALKYDIKSHLLNRKKR"
     gene            complement(12193..14865)
                     /gene="harA"
                     /locus_tag="POH73_000414"
                     /pseudo
     CDS             complement(12193..14865)
                     /gene="harA"
                     /locus_tag="POH73_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
     gene            14864..14953
                     /locus_tag="POH73_000415"
     CDS             14864..14953
                     /locus_tag="POH73_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886393.1"
                     /translation="MLLTTPIRWYLIDNNYQYQLLVLIVAHPN"
     gene            complement(15199..16866)
                     /locus_tag="POH73_000416"
     CDS             complement(15199..16866)
                     /locus_tag="POH73_000416"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDI6886394.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVQGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWEKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            complement(17306..19012)
                     /gene="acsA"
                     /locus_tag="POH73_000417"
     CDS             complement(17306..19012)
                     /gene="acsA"
                     /locus_tag="POH73_000417"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDI6886395.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRLFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            19181..19813
                     /locus_tag="POH73_000418"
     CDS             19181..19813
                     /locus_tag="POH73_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6886396.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTHEDLKALTFDAYLTAFRD
                     AEDQFEALLEITTLPEGRIYVARQDKLIVGYVTFHYPDEIERWSTGNLPYLIQLGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFEDYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSNPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            19838..21007
                     /locus_tag="POH73_000419"
     CDS             19838..21007
                     /locus_tag="POH73_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HDI6886397.1"
                     /translation="MQQHLSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAMKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKALNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDSSFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADTYTNGKIIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(21105..22094)
                     /gene="ccpA"
                     /locus_tag="POH73_000420"
     CDS             complement(21105..22094)
                     /gene="ccpA"
                     /locus_tag="POH73_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDI6886398.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGKLIEKGAKSFALVGGEHSKKAQEDVLAGLTEVLNKNSLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(22167..22280)
                     /locus_tag="POH73_000421"
     CDS             complement(22167..22280)
                     /locus_tag="POH73_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886399.1"
                     /translation="MKKVYIKLKYIVRYHLAFVFISGFSLNFSKQKYVTVN"
     gene            complement(22634..23725)
                     /locus_tag="POH73_000422"
     CDS             complement(22634..23725)
                     /locus_tag="POH73_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HDI6886400.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLSDLYNAKKLK"
     gene            complement(24180..24263)
                     /locus_tag="POH73_000423"
     CDS             complement(24180..24263)
                     /locus_tag="POH73_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886401.1"
                     /translation="MLFETSLDNNSDIAHFKLMMEKYLIIK"
     gene            complement(24419..25609)
                     /locus_tag="POH73_000424"
     CDS             complement(24419..25609)
                     /locus_tag="POH73_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886402.1"
                     /translation="MTQQQNNKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAFTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQTETDKSAAVSNEESKASALKAQQAAI
                     KEEASANNLSDTSQEAQAIQEAKKEAQAETDKSADVSNEESKASTLNVSKEESQAERL
                     ANAAKQKQAKLTPGSKESQLTEALFAEKPVAKNDLKEIPLLVTKKNDVSETVNTDNKD
                     TVKQKEAKFENGVITRKADEKTPNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQ
                     KKQHNKKASQGAKKQSSSSNSTTKTNQKNSKATNAKSSNASKKSNAKVEKAKSKIEKR
                     TFND"
     gene            complement(25683..26174)
                     /locus_tag="POH73_000425"
     CDS             complement(25683..26174)
                     /locus_tag="POH73_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDI6886403.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            complement(26248..27561)
                     /locus_tag="POH73_000426"
     CDS             complement(26248..27561)
                     /locus_tag="POH73_000426"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDI6886404.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKESDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVILFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            complement(27585..>29698)
                     /locus_tag="POH73_000427"
     CDS             complement(27585..>29698)
                     /locus_tag="POH73_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDI6886405.1"
                     /translation="TENVQNEAAESKQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVM
                     TPSDKKRMMDRKKHSKVNVPELKPVQSKQAASESKTATQNTPSSSTDSQESNTNAYKT
                     NNMTSNNVENNQLIGHAATENDYQNAQQYSEQKPSADSTQTEIFEESQDDNQLENEQV
                     DQSTSSSVSEVSDITEESEETTHQNNTSGQQDNDDQQKDLQLSFSNQNEDTANENRPR
                     TNQPDVATNQAVQTSKPMIRKGPNIKLPSVSLLEEPQVIEPDEDWITDKKKELNDALF
                     YFNVPAEVQDVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGT
                     SRVGIEVPNQNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALI
                     AGATGSGKSVCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVK
                     AATQSLKWAVEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMA
                     PQEVEQSIARIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRT
                     ILDSGGAERLLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEE
                     KELLKKTQTQSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYV
                     SSANGSKPRDVYVTEADLNKE"
BASE COUNT         9410 a         5395 c         4347 g        10546 t
ORIGIN      
        1 taaaaatgat agattcaaga ttatatcgag ctaccctatt ttacaattaa cttagaattt
       61 acaagtctta tttgttgaat tatctacaaa tttcatcatt tctttttaaa aatatcaatt
      121 gcgatatacg aatccttatc cctctatcaa ataaccattc atcgtcttac ttaataaata
      181 atttcggatc atctgtaatt aatccatcta cacctatttg tcttaatttt tctccagttt
      241 tcaatttatt aactgtgtat ggcataacct gtaattgatg atgatgtgct tcatcaacaa
      301 attttctggt cactaatgca taattaggat taacataact agcaatttga gcaattcttg
      361 aaaagtttgg ctttttatac caatacttac gtttactaca aagcacacct aattcatata
      421 tactgcctaa tgtgtttaac ttttcgatgc attcgatatc aaaagattgt ataactactt
      481 gtgtcgcatc gactttcttt tcttccaaaa atgccagcaa tttacattct attcctggat
      541 ataaattcgg acttttcaat tcaatcaata gctttttatc aaactttaag cataatgaaa
      601 gtacttcatc taacgtgggt attcgttctc ccttaaaagc aacatcttta taactaccaa
      661 aatcaaatga ttttaattgc gataatgtgt aatcagcaat acgcccctta ccatccgatg
      721 ttctatcaat tgtttcatca tgtatcacaa caaaatgttg gtctttggtc aaatgaacat
      781 ctatttctaa catagcaaca ttgagcccca ttacctctcg ataaccgacc attgtatttt
      841 caggaaaatc actcggcaat ccacgatgcg aaacaatttg taattcatct ttcagtttat
      901 tcagagtcat taataagtca cctttctgtt aaactttgat aaactattct aaaattaaac
      961 tatcacaatt atattggagg ttaaagcaat tgcatcaaca tgactttaaa gtcaaaactt
     1021 cttggcaagg tggtcgtaac aatgtcggaa acgttcaagg cgacatactt tcagagaata
     1081 tttcaatacc tgcttcttta ggtggtgttg gtataggaac aaatcccgat gaaatgttag
     1141 tatcagccgc gtcatcatgt tatatcatct cattagcagc tactcttgaa cgtgcaaagt
     1201 tcacagatat ttcaattgaa caacaatcga ttggaacagc ttgtttaaat aatggaaaat
     1261 tcagtatgtc aaaaattgtg caccatcctc acattcaaat tccaagtgat caaatagcac
     1321 aattagaaaa gcgattacca aaattgataa caattgcaga taataattgc atgatttcaa
     1381 atgctgtaag aaataatgtg gacataaaaa tctatcccat cattcaggcc aaataaaatc
     1441 gttaagtgat aacatttgtt cgacaatata gcgttatcac tttttttagg cttttaaatt
     1501 ttcagaaaat ttgctaaaat agtgaaatct tatttatagg aggcatataa atgtattatc
     1561 atcaaccgtt gttattaaca cctggcccaa cccctgttcc tgatgccatt atgagagaaa
     1621 ttcaagcacc tatggttggt catcgttcta aagattttga agacatcgct caacaagcat
     1681 ttcaaggtct aaagccaata tttgggagtc aaaatgatgt acttatatta acatctagcg
     1741 gtacaagcgt cttagaagct agtatgttga acattgtaaa ccctgaagat cacttcgttg
     1801 tcattgtttc aggtgccttt ggtaaccgat ttaaacaaat tgcacaaact tattacaaaa
     1861 atgtacatat ttatgacgta acatggggag aagctgtaga tgtcaaagat ttcatcaatt
     1921 acctttcaac tttaaatgtt gaagttaaag cagtatttag tcaatattgc gaaacatcta
     1981 cgacagtgct acaccctatt cacgagttag gaaatgccat caatcaattt aatagtaata
     2041 tttattttgt agttgacggc gtaagttgca ttggtgctgt tgatgttgac attaacaaag
     2101 ataaaattga tgtacttgtt tctggtagtc aaaaagcaat tatgttacct ccaggattag
     2161 cttttgtagc ttatagccac cgtgcaaaag aacgtttcaa agaagtaact acaccaaaat
     2221 tttatctaga cttaaataaa tacatttcgt cacaagctga caattctaca ccgttcacac
     2281 caaatgtgtc tttatttaga ggtgtaaatg catacgttga aaccgtaaaa gcagaaggtt
     2341 tcaatcacgt aatagcacga cactatgcaa ttagaaatgc attaagaagc gccttaaagg
     2401 cattagattt aactttatta gtcaatgata aagatgcatc tccaacggtt acggcattca
     2461 aacctaacac aaatgatgaa gtgaaaataa tcaaagatga acttaaaaat cgctttaaaa
     2521 taacaattgc tggtggtcaa ggccatctta aaggtcaaat tttaagaatt ggtcatatgg
     2581 ggaaaattag tcctttcgat attttatcgg tagtatctgc tttagaaatt attttaactg
     2641 aacaccgtaa agttaactat atcggtaaag gcatatcaaa atatatggag gttattcatg
     2701 aagcaattta atgtactcgt tgcagatccc atatcaaaag atggtatcaa agcattatta
     2761 gatcacgaac aattcaatgt agatattcaa actggtttgt ccgaagaagc attaatcaaa
     2821 attatacctt cataccatgc tttaatcgtt cgtagtcaaa ctacagttac tgaaaatatc
     2881 ataaatgctg ctgattcttt aaaagtaatc gcacgcgccg gtgttggtgt agataatatt
     2941 aatattgatg ctgcaacatt aaaaggtatt ttagttatta atgccccaga tggtaatacg
     3001 atttcagcta ctgaacattc actagcaatg ttattatcaa tggctcgaaa tattccgcaa
     3061 gcacaccaat cacttacaaa taaagaatgg aatcgaaatg catttaaagg tactgagctt
     3121 tatcataaaa cattaggtgt cattggtgct ggtagaattg gtttaggtgt tgctaaacgt
     3181 gcacaaagct tcggtatgaa aatactagct tttgaccctt acttaacgga tgaaaaagca
     3241 aaatctttaa gcattacgaa ggcaacagtt gatgagattg cccaacattc tgatttcgtt
     3301 acattgcata caccactaac acctaaaaca aaaggcttaa ttaatgctga cttttttgcc
     3361 aaagcaaaac ctagtttgca aataatcaat gtggcacgtg gtggtattat tgatgaaaag
     3421 gcactaataa aagcattaga cgaaggacaa attagtcgag cagctatcga tgtgtttgaa
     3481 catgaacctg caactgattc gcctcttgtt gcacatgata aaattattgt tacacctcat
     3541 ttgggtgctt caacagtcga agctcaagaa aaagtggcaa tttctgtttc aaatgaaatc
     3601 atcgaaattt taattgatgg tactgtaacg catgcagtga atgcacctaa aatggactta
     3661 agcaatattg atgatactgt aaaatcattc atcaatttaa gccaaacagt tggtgaatta
     3721 gctattcaat taatgtacaa tgcaccaagc tctattaaaa ttacctacgg tggcgactta
     3781 gcctctattg atagtagttt attaacacgt acaattatta ctcatatttt aaaagatgat
     3841 cttggtcctg aagtcaatat tatcaatgct ctaatgttgt taaatcaaca acaagtgaca
     3901 ttaaatattg aaaataataa agcagagaca ggttttagta actacttaga ggtagaatta
     3961 tcaaacgata gcgattccgt taaagttggt gcttctgtct ttacaggttt cggtccaaga
     4021 attgttagaa ttaataattt ttctgtagac ttaaagccaa atcaatatca aattgtgtca
     4081 tatcataatg atacaccagg catggtaggt aaaactggcg cattgttagg taaatacaat
     4141 atcaacattg catctatgac tttaggtaga actgaagcgg gcggagatgc gctaatgatt
     4201 ttatccgttg atcaacctgt ttcaaacaat ataattgatg aacttaaaca agttggtgaa
     4261 tacaatcaaa ttttcacaac tgaattgacg gtacagtcat aaacaattta tataaattca
     4321 aaatgccaag aagacattgg attacttcca ttgcttcttg gcattttttt aatattaatt
     4381 tcattgtaac tcaatatgaa acctttggtt caatcattca cttcaagtta tgctgtcaaa
     4441 caacaatcaa attacaaatt atctagtaca cctctaagtt cacctaaatg attaataaca
     4501 tagtcggcat gatgcgcttc taactcacct gcagcatcct ttccttttaa acctgataat
     4561 gttccaataa acgttgcacc tattttttga gcacttaata agtcagctaa cgaatcgcct
     4621 actataaata cgtcatcttt atttacaatg ttatcttgct tattgatata agattcatat
     4681 ttatcgcgtt tattaccatt taaagctgcg atataactaa aaggattcgg ctttcctaat
     4741 ggtcgtgctt gcggatacat attctctgct tctaaaacat cacttgctgt tgcaataaaa
     4801 tcagcttcaa aatacggtaa caatcctaaa ttttcaaatg gcacaacagt ctcagtataa
     4861 ggacgacctg ttgctatacc taattcgaaa ccagcacctt ttaaatcatt cagaagtacc
     4921 ttaacttcat ctaccggtct caaaataatt tcttgataaa tataacctgt cttaaaagta
     4981 gttcgtgcta ttttcttttc aacctcttca tacaacttcg atcctaaata ccattcttga
     5041 taaacttctt gcgctaacgt ccataatgca cccttcaaac taaataaatt agcatccgaa
     5101 acatgtaact ctgttgttgc aaactcttcc aaagcagcat aaatattatt tttaccaact
     5161 tttacattat ccaaaaattg taaaggtagt tgttcattta aattaaaacg gtctgctatg
     5221 tttgtgctta tattttgcaa cttcaattcg acaggctcat ctcgatacat gaatgcctca
     5281 atttcatcat gtgataactt tttcaaaata tcaattaaat gaatactaaa aacgataaat
     5341 aacatatccc aatttgaatt cagccctagc gattttaatt tgtttaaaat cttatctttt
     5401 tgaaaaattc gattcctaat gtcttgtata tcgttatcag tcaaagtttc ccaatctata
     5461 tgtgaatgaa gacctaaata acacttatcc attaataatt catataccgt taatgcagag
     5521 acatcgaaac aacgttcttc acttaaaaaa acgccatcaa catcaaataa aattttcttc
     5581 acaatcccca ctccatttct gaaaattcag atataaatca ttctactatt tgactaaaaa
     5641 aagcgcaaac cctattgagt tggatttgcg ctttagctgt ctaaatttta taaatgtttt
     5701 tcaatttcat cagcaacctg ctgaacgtgt gtaccaacaa taacttgagt tgaatgtttg
     5761 ccattaacag taacaccaac tgcaccggcg tttttaatct tctgttgatc aataatagat
     5821 gtgtctttta actctagacg taaccttgtt gcacaattgg ttaaattaac aatattctct
     5881 tgaccaccta aaccttctaa tatttgtata gcatgattat gatatttact ttgtttaata
     5941 tcattttcac caggagcaat attatctttt acaactgttg gatcaactaa ttcattttca
     6001 cctctaccaa tcgtattcaa gttaaatact tggattacta cacggaaaat cacatagtat
     6061 aagatgaaaa atacaacacc ttgaacaagc aacatcaatg gatgatttga taccggatta
     6121 attagtgata acacataatc tatcaaacct gcactaaatg aaaatccagc tgtccaatgg
     6181 aatgtagcta cgataaataa agataatcct gttaataacg catgaacaac atataagatt
     6241 ggcgcaacaa acataaatgc aaactcaatc ggctctgtaa caccaacgaa aaatgctgca
     6301 actgaactcg ctaggaacca accgtaaact tgttttttct gagtagtttt agctgtatga
     6361 tacattgcta acgcagccgc tggaataccg aacatcatga ttgggaagaa tcctgcttga
     6421 tagcgtcctg taataccttt tatagcatct ttgccacttt ggaatttacc aatatcatta
     6481 ataccaatcg tatcaaacca gaacacacta ttcagtgcat gatgtaatcc tgtaggaatt
     6541 aataatctgt tggcaacacc atatacgaaa gccccaaatg atcctaaacc aactatagat
     6601 tcaccaaatt ttacaatcca tgaataaagt agtggccata agaataacaa tatgacaact
     6661 aaaaatgtac agtaaaatgc agtcataatt ggaactaaac gtttaccact aaaaaatgat
     6721 aatgctaatg gtaattctgt ttcactaaac ttattgtatg cataagctgc tattaaacct
     6781 attacaatac caacaaagac attgccatta ttcatctttt caaaagctga atttatttca
     6841 gaagctttca ttcctaataa aggcgctaat ttcattggtg ataatacaac tgtaactaaa
     6901 aaatatccta acgcagctgc aagcgcaact gcaccatcat ttttctttgc cattcctata
     6961 gctacaccaa ttgcaaataa aatacctaat tgctctaaaa tcgtagtacc taccgtagta
     7021 aagaacattg cgattttcgg cgccgcatga agtgcattta acgtattacc aattccggca
     7081 ataattgctg cagccggtaa aatggcaact ggtaacatta acgaacgccc taaattttgg
     7141 aaaaatttat acattgaatg tcatccttct taaaataagg tagaaatata aagattacta
     7201 atgtaactag aataactact tcgatactcc gttatagtca cctaggctta ctaaccagct
     7261 atatttctac ctcaagttat tttataaact ttttacaatt tcatgcaatt cttgttgtaa
     7321 ctttgctgtt cgtgtttcaa tctcttttgt aatgtaatcg atacgctcgt ttcgttttaa
     7381 atctttaggt aaatcgttaa tatcgattgg tttaccaata tttatgtatg cttgtccagt
     7441 tataagacca tgaatcttag taggaccaac ataagcaaca ggtaatattg gtgacttact
     7501 taacattgca attgttgaag caccacgttt caaaggtgca ccttcttgcg atgtgcgaga
     7561 acctgttggg aagataccaa ctgtcttatt atctttcaac aaattgattg ggcgttttaa
     7621 agtactaggt cctggatttt cacgatctac aggaaatgca tttaaagacg ttaaaaattt
     7681 accaatccat ttatttttga ataattcttt tttagccata taatgaattt gattaggata
     7741 taatgccata cctagcataa tgacttcgtt ataactttca tgcgtacaag ttacgacata
     7801 tttactatcc ttaggaatat tatctttacc gattacgtat aatgattttg acattttaac
     7861 taaaatgaaa ttcaaaatct tactaatcac tgaatacatt gtgccaccta cttaacttat
     7921 ttgtttataa caactagatt ttatcactat atttttacta gaacaagata gtctgaaaat
     7981 acatctaaag ttgtatagtg tattactagc aataattata aaaaaaagac ataattaaag
     8041 catatataaa tcatataatg gaggttaagt atgtcagatt ttaatcatac agatcattca
     8101 acaacaaacc atagccaaac acctagatac agaagaccta aatttccatg gtttaaaaca
     8161 gtcatcgttg cattgattgc tggaatcatt ggtgcacttc tagtacttgg tataggcaaa
     8221 gtattaaata gtacaatttt aaataaagat ggttcaactg tccagacaac aaataataaa
     8281 ggtggcaatc aattagacgg tcaaagcaag aaattcggta ccgttcatga aatgataaaa
     8341 tctgtctccc ctacaattgt tggagttatt aatatgcaaa aagcatcaag tgtagacgac
     8401 ttattaaaag gcaaatcatc taaaccatct gaagccggtg taggctcagg tgtcatctat
     8461 caaataaaca acaattcagc ttatatcgtt acaaacaatc atgttattga tggcgcaaat
     8521 gaaattagag tccaattaca taataaaaaa caagttaaag cgaaattagt tggtaaagat
     8581 gcagtaactg atattgctgt acttaaaatt gaaaatacaa aaggtattaa agcgattcaa
     8641 ttcgccaact cttcaaaagt acaaactggc gatagcgtat tcgcaatggg taacccatta
     8701 ggattacaat ttgctaactc tgtaacatct ggtatcattt cagcaagtga acgtactatt
     8761 gacgcagaga caactggtgg taatacaaaa gtcagcgttc ttcaaacaga tgctgctatt
     8821 aacccaggta actcaggtgg cgcattagta gatattaatg gtaatttagt tggtattaac
     8881 tcaatgaaaa ttgctgcgac acaagttgaa ggtattgggt ttgctattcc aagtaatgaa
     8941 gttaaagtaa caattgaaca acttgtaaaa catggtaaaa tagaccgccc ttcgattggt
     9001 attggtttaa ttaatttgaa agatatacct gaagaagagc gcgagcaact tcatactgat
     9061 agcgaagacg gtatttatgt cgcaaaagct gatagtgata ttgatcttaa aaaaggtgat
     9121 attattacag aaattgatgg caagaaaatt aaagatgatg ttgatttaag aagctattta
     9181 tatgaaaata aaaagcctgg tgaatcagtc actgttaccg ttatccgtga tggtaaaact
     9241 aaagaagtta aagtgaaatt aaaacaacaa aaagaacaac caaaacgtca aagccgatca
     9301 gaacgtcaat cacctggcca aggcgataga gatttcttta gataatttat agacagaaaa
     9361 gtacattaag agcctactca tattcattcc taagaatgta atgagtaggc tctaatttat
     9421 ttagctatgc aattatattt attgatagtt aaccatgaag tattttttct taccgcgacg
     9481 aataatcgta aattcgccat caattttatc ttctggtgct aaagcataat taacatcttg
     9541 ttgtctctca ccattaatat aaatcgcacc attgttaaca tcttcacgcg cttgtcgttt
     9601 agaaggagaa atgcctgttt caataaggac ttcaacgata tttgttgtgt catttgataa
     9661 tgtcacttga ggcacatctt taaatccatc ttttaattct ttcgctgata atgattttaa
     9721 atcaccacta aataatgctt gtgaaatacg gattgcatca tttaatgcat cttcaccatg
     9781 aataaattta gttacttctt cagctaatgt tttttgagct tcacgtaaat gtggtgcttc
     9841 atttttagat tgttctaagc gatcaatttc ttcttttcct aagaaagtaa agtattttaa
     9901 gaatttaatt acatcttcgt ctgattgatt aatccagaat tgataaaatt cataaggact
     9961 cgtcttttca gcatctaacc aaacagcacc tgactcagat ttaccaaatt tcttaccatc
    10021 tgatttagtt acaagcggaa tagttaaacc gtatgcgtct gtttgaccat acatacgacg
    10081 cattaattca ataccacttg tgatattacc ccattgatct gatccaccta cttgaatctt
    10141 acaattcaat tctctattca aatgaccgaa atcaatagct tgtaaaatcg tgtatgtgaa
    10201 ttctgtatat gaaataccat gttctaaacg actttggatt gaatctttac ctaacatgta
    10261 attaacgccg acgtgtttac catagtcacg taaaaagcta attaatgaaa tttgtcctaa
    10321 ccagtctcta ttattaacaa gcactgcacc atggtctgtt ccaaattcaa aaatattatg
    10381 catttgctta ctaatacctt cgatattttt atctacttgt tcttctgttt gtagcacacg
    10441 ttcttctgat ttacctgatg gatcaccaat catacctgta ccaccgccaa ttaaaacgat
    10501 aggacgatgt ccatgttctt gaaatcttct taatgttaaa aatggtagta agtgaccaat
    10561 gtgtaagcta tctgccgttg gatcagcacc gcagtataac gtcacttgtt ctttatttaa
    10621 taaatcttca ataccttgtt catcagtttg ttgataaata agacctctcc attttaaatc
    10681 ttcaattaat acattcgtca ttattatttc ctcctattta taattattta gtgaatactt
    10741 gttaaaatac tttaaagctt tttgaacgta aaaaaaccct tacaacaaat atgtaagggc
    10801 gcgattgcac gttaccacca aacttaaaca taatcataag ataatgttca ctctattaat
    10861 gatacgttca ttaataaacg taggacatgt tagttataaa ggtgtattca tattattaaa
    10921 aacactagtt cacagcgacc actagctctc tgatgatttc aaataatatt acttgtcctt
    10981 ttatcctatt ctttatgcat tttccctaat tataattaac gttaaaataa aagtcaaatt
    11041 gcttaaatat ggtatactat acgtaattta ggaggttaag gatgacgaat caagacaaca
    11101 atcatcaatt gaatcatcgt atatatcaat ttgaaaagat atataaagct atcaaacatg
    11161 tcattgtttt tatatttatg attttcattg ccatcgttgc tatcgctgtg attgcgatgt
    11221 ctttatattt tcatcattta actaaaacgt ccgactcatt atcagatgat gctttaataa
    11281 aaaaagttcg acaaatacct ggcgatgaat tattagatca taataacaaa aatttgttat
    11341 atgagtataa ccattctcaa aactcactca ttataggacc taaaacatca agtccaaatg
    11401 tcattaaagc attaacgtca tctgaagaca ctttatttta taaacatgat ggcatcttac
    11461 caaaggcgat tttaagagca atgatacaag atatttttaa tactaatcaa agttcaggtg
    11521 gtagcacaat tacacaacaa cttgttaaaa atcaagttct taccaacgaa aaaacatata
    11581 gtagaaaagc aaatgaactt cgcctagcaa ttagattaga acacctactc tcaaaagatg
    11641 aaattatata tacgtattta aatatagttc ccttcggtag agattataat ggcgctaata
    11701 tttctggaat tgcatccgct tcatatagtt tatttggtgt tccaccaaaa gatttatcaa
    11761 ttgcacaatc tgcatacctt atcggtttgt tgcaaagccc atatggctat acaccctacg
    11821 aaaaagatgg aacattaaaa tcggataaag atttgaaata tagtattcaa agacaacatt
    11881 atgtattaaa gcgtatgtta atcgaagatc aaatcactaa aaaagaatac aacgacgcat
    11941 taaaatatga tattaaatca catttgttaa atcgaaaaaa gcgttaattg atgctcactc
    12001 ttttaagtaa ccacaataat ccatccaaat attaaaaaca gcagtaagat tattttcaat
    12061 tagaaaattt ctcactgctg ttctttattt taaatactct aaaggaaagc tattaaacaa
    12121 atcaatagtt tataatttaa ttgctttcac attgtctaca aagctaatca tacatatgct
    12181 atatccagtt aattacttag attcttttct gaatttagga atgaataaag ctaacatacc
    12241 taataacgca tataaacccc accatgattg gcttgaagtt gtttctccag tttttggtag
    12301 cattttagat tgactaagtt tgttttcttt cgatggtgtg ccggcctttt cttttgtttt
    12361 cactgttttg tctacagttt tatgaatatc actcggcaga tgttcagttg tttttttatc
    12421 agtaactttg tctgtattat tataattttg tctcacgcca tcaagctttg ctgcttttcc
    12481 agtatgattt ttatcggtat tatgtgctag ttgtataact ttgccttcat ttttattaga
    12541 gtctttgtca gtatcgacat tagatgtcat tccaaggcta ttatcaacac ccttatcagt
    12601 gtctttagca atttgagtat ccatttcttt gaaatcatac ttatcgaggt gagtgttatc
    12661 cgacatatcg gataaatgat caacatcatt ttgcacatct ttgtcaacac tactatcagc
    12721 atctttaacc acgtcagact ctggttctat cacatctgct ttatcagacg catcttttgg
    12781 atttgttgca gtgctgctat tttcagctgt atccgttgca gtaaccttat cttcttgtcc
    12841 tgtttgtaca tttagaggtt caatcgtgtt attttgtgat gtgtcatcat cttttgtctt
    12901 gatatcctga tttacaattc tgacatgata ttgaccttca taaccaatgt ttgccacaac
    12961 gactttaaca atcgcattat aaattgcttt gtcaggtaca tatgggaaaa tcagcgttct
    13021 agaattattt ttaggatctt tagaaacagt agtgacacgt ttaccttcta caattaaatc
    13081 tttccagtaa ctgtcatcct ttgttttcat cactacatat ttttgaccat ttaaagttgc
    13141 tgtatagaat ggatgttcaa caaatccatc catgactgac tcactatttt cttcactttc
    13201 aaaaacaaca aaatgagcct cttgtacatc tgttaattga tcatttgtag gtgtaacatt
    13261 ttcaaattct gtcactgcag acttaacttg atcggctaac tctactctag tttgatctaa
    13321 tttcttttta tattctgctt tatatttttc cggcaattta tcttgtaatt tttctaattc
    13381 ataaacttgt ctttctaatg ttttagcttt gtgatacgga gctaataatt tttgtaaatt
    13441 gtatgtttcc tcatccacat agtcgtctgg gttattagta ataggctgtg caaagaccat
    13501 taatgtataa tcataatctt catggaagtt ctcaccatat tcaatagatg acacaatttt
    13561 aacttctctc gccccattag aaactggaaa acgaatatag gcataatctt tatcagaatc
    13621 atatgatact aattcgactg gtaacttttt gtcaccttca taaacttcaa atttcttcca
    13681 tgttgaagct gtctttaaac ctaattcaat tattggtcct gtttttgtaa aaataacagt
    13741 tgctggttca acagtactag cataatgata aaactgtctc tcacctttat catttttcat
    13801 ttgaaaatca attggtcgcc aatgatcagc tgtatgttct ttatcgatga tagccgggtt
    13861 tttaatggca tcttgtagtg attcatctgc tggtggatac tgttggtacc catttgactg
    13921 attacttgat gcattcgttg catcgttagt attgtcatta gaatttgttt catgagctgg
    13981 ttggcttgat gcttgatctg aattcgctga cgcttttggt tgtgcaggtt aactcatatt
    14041 gttcgttgcc tgcggttgat cattagtatt gttggtcgtt gatgtatttt gattagatgt
    14101 attcgtgttt gtttgattac ttgcgtctga acttgattga tcattcgttg ctactgcatc
    14161 atttgtatct gattttacaa gtgaaggatc gttataaata ggtttagcaa atactaattt
    14221 agtataatca taatttgttt cagctccatc atcaatttga gttgaagaga caattttcag
    14281 ctcttgtgta ccatctgaaa cagggaatcg aatataggca tggtcttctg gaacaggact
    14341 atacgataca agtctcactg gcaatttttg attgttttca taaacttcaa acttcttcca
    14401 tgttgaagca gtattgatgt ctaattcaac ctcagctttc tttttagtgt aatatacatc
    14461 tgctggatct ttgatgctga aaaagtgata gtactgcgtt tcattgtttt tatctaataa
    14521 ttgaaaatta acttgttctc ttggacctat atcatgttct ttattttcta ctgcaggatt
    14581 tttaattgca tctttaagtg attcatccgc tgcaggatag gttttcgcag tgtttgttgg
    14641 ttgcgtagca ggttgtgttt gatttgtatc ctttggtggc tgagttgtag ttgcattatt
    14701 attttgattt tccgagattt tatctgaagt atttgtattt tctgctgctt gcgcttgatg
    14761 ttgagaagta attaaaaata gtgtactgac aatgaccgat gcaacgccta gagttgattt
    14821 tctaatagaa tagaaagacc ttaattttgg gtgatgtttg ttcatgttgt taacaactcc
    14881 tataagatgg tatttaattg ataataatta tcaatatcaa ttattagtgt taattgtagc
    14941 gcatcccaat taaatattgc aacctatttt attaaaaatt tttaatatga atgttcattg
    15001 catcgctttc ctattcttca agcccacttt ttatcttgga atacattaat taaccatctt
    15061 caaacattgc cgttagattc gccaaattga atctattttt agcacagcaa aaaaaccgaa
    15121 ctgctgaaat aatgcatctc caacagtccg gtctattaaa ctatttactt atctttatta
    15181 aacaattgac atgatgtatt agaataaccc aattgcatga ccatcatcag taacatccat
    15241 gtttaatgct gctggttttt taggtaaacc aggcatagtc atgattgcac ctgtcaacgc
    15301 tacgataaat cctgcacctg ttttcgcttc taattcacga attgtaattt caaatcctga
    15361 tggtgcacct aacaacgttt gatcatctga gaatgaatat tgtgttttcg ccatacatac
    15421 tgggtaatta tcccaaccat tttctttaaa ttgttttaat tgtttttgcg ctttactact
    15481 aaacgttact tttgaaccgc catagatttc agtcacaatc ttttcaatct tttgctctaa
    15541 tggtaattct aattcatata aaggtttaaa tgaattaggt tgatcaatga cttctaatac
    15601 ttcatttgct aagtcaacgc cacctttacc acctttttcc caaacttcag ttaaggcaat
    15661 tcgtacgtta ttttctttag cccaagattt tacatattct acttctgcat cggtatcatg
    15721 tataaatgca ttaattgcaa caaccggttc tacaccgaat tttttaatat tattaacatg
    15781 acgctctaaa ttaacaattc ctgcttttac tgcttctaca ttttcttctt ttaaattatc
    15841 ttttgctaca ccaccatgca tttttaatgc acgaatcgtt gctacaacta caactgctgc
    15901 tgggtcgaat ccagcttcac gcgccttaat gtccatgaat ttttcagcgc ctaagtctga
    15961 accaaatcca gcttccgtta caacgatatc agctaaatca cgtgctgttt cagttgctaa
    16021 aattgagtta caaccgtgtg cgatattcgc aaatggtcca ccatgaacta atgctggtgt
    16081 cccttcaatt gattgtacta agtttggttt aattgcatct tttaaaatca ttgcaagtgc
    16141 accttgcact tttaaatctg caactgtaac tggcttgcga tctctagtgt aaccaatagt
    16201 aatacgacta attttatctt ttaagtcttt aatacttcta cttaagcata aaatcgccat
    16261 aatttcagac gctactgtaa tattaaagcc atcttcacgt ggtacaccat ttgtaggtcc
    16321 acctaaccca acgtttacat gtctaagtgc acgatcattc atatctaata cacgtttcca
    16381 ttcaatacgt ctttgatcga ttcctaattc attaccttgg tgaatatgat tatcgataaa
    16441 cgcagacaat gcattattag cagttgtaat cgcatggaaa tctccgttga aatgtaagtt
    16501 gatatcttcc ataggtaaga cttgcgcata accaccacca gttgcaccac ctttaatacc
    16561 aaatgttggt cctaaagcag gctctcttaa tgcaaccata acgtttttat ttaactcatg
    16621 gaatgcatca gctaaaccaa ctgtaaccgt tgatttacct tcaccagctg gtgttgggct
    16681 catcgcagtt actaaaacaa ctttcccttt gttttctctt ggcgtaattt tattaatgtc
    16741 gattttagct ttgtaatgac cataaggttc taatgcatcc tctgaaatac ctactgatgc
    16801 agcaatatcc ttaattggtt gtagtgttga ttgattcgca atatctaaat ctgataaatg
    16861 agtcaacttt tttcgcccct ttttgtttaa tttatgcatt tataatatgt aagttgtagc
    16921 taagaaattt ttacttacat tcatataata acgaactgtt aaaaacttgt cgactaaatc
    16981 gggttagcgc tttcattttt gcaataaatt cacttattaa agttttgtat tatacaactt
    17041 ttagcgcttg atactattga taactatatt tttctattat ttaaaatagt catcttccaa
    17101 aggtcatatt aaaaataaaa agcaatataa agatatgcca ttattgttat gtcatcattt
    17161 tcaaatgttt tctacgaaat gatatgacac aagttaacag ctctttatat tgcttatcgt
    17221 aaagtaaacg attatttaaa cattaacatg aaatatgttg tcacccttta ttcttcgaaa
    17281 gaaatcttct ataacattca tgtcattatt ccattgtact taaatcccct gcatctaaat
    17341 ttaattccca agcttttaat acacgtctca taattttacc tgagcgtgtt ttaggtaatt
    17401 tatctttaaa ttcgatttca cgtggtgctg catgcgccga caaaccttct ttaacaaata
    17461 aacgaatttc ttcttttaat tcgtctgttg gttcatatcc ttttctcagt gcaacaaacg
    17521 ccttaattat ttcaccgcga accggatcag gtttaccaat aattcctgct tcggcaactg
    17581 cttcgtgttc aaccaattta gactcaacct caaatggccc aactcgttcg ccagctgtca
    17641 taattacatc atcaacacgt ccttggaacc aaaagtaacc atcttcatct ttatatgccg
    17701 aatcaccaga tacataccag tctccaataa aatatgactt atatttttct ggattcttcc
    17761 agatacgata catcattgat ggccagcctt tttttatagc aaggttgccc attcgatttg
    17821 gtggtaattc attacctgca tcatcgataa ttgcagcttg aataccaggt aatggtttgc
    17881 ccattgagcc aagcttgacg tccatcgttg gatagttaac aatcatatgt ccacctgttt
    17941 ctgtcatcca ccaagtatct aacaccgtta aaccgtatac ttttttcgcc cattttataa
    18001 cttcaggatt taaaggctca cctactgata gaatcgaacg taacgatgac aagtcatatt
    18061 tctcaacaat atcgtcacca gcactcatta acattcttaa agctgttggt gccgtatacc
    18121 aaatcgtcac tttaaaatct tcaatcatac tataccattg ttctggtgaa aagcgaccac
    18181 cagctataca atttgtagcg ccatttaacc atggtgcaaa aataccataa gatgttcctg
    18241 taacccaacc tggatctgct gtacaccaat aaacatcatc ttcttgtaaa tctaatacat
    18301 attttccaga aatatagtgc actaacatcg cttgttgaac atgcaataca cctttaggtt
    18361 gcccagtaga acctgatgta taatgtaaaa tcaaaccatc atccgacttt aaccattcaa
    18421 tgtcaaattc atcgctagca gtttccatca aactaatgaa gtctatgtaa ttgtcttcta
    18481 catcctcatc tacgacaaca atttttttca agttcggtaa tttatctaca ggtactcgag
    18541 gtaacaatgc cttattagta attaacactt tagcttcact gttctctaat ctatccgcaa
    18601 ccgccttttc cataaatgct tcaaataacg gcccaacaat tgcaccaatt tttaaaacac
    18661 ctaacaacgc aaaatatagt tcaggtgtac gcgacataaa tataaatact ctgtcacctt
    18721 tgtcaacttc tgcatgttca gacaaaacat tcgccgcttt attagataac cgttgcatat
    18781 ctttataagt atacgattct tttctgtgtt catctttgta atttaatgct attttatccc
    18841 ctaatccttg atctacatgg cgatctatac attcatatgc catgttcatt tttccagttt
    18901 cactccaaga aaatgcttgt tctacgtctt tccaatcaaa agtattatat gtttcttcat
    18961 aatctttaag gttatgtttg ccttgcgctc ctttataaac ttcgactttc atttgaaact
    19021 ccccctttgt tatgtgaaaa cgctttcttt ctcaatatta tacataacat ttctaattat
    19081 tcaaaatttt tacttttatc ctttttctag tattaattat tgtaaatttt ctatataatg
    19141 aatttaatac aatcacgacg tcgattgagg agttgaaaat atgaatcatt taaagacgta
    19201 tcaatccgaa gattattaca tacatgacaa gcaatttgtt attgaaggtc ctttaacaca
    19261 cgaagatttg aaagcgctta ctttcgatgc gtatttaacc gcatttagag atgctgaaga
    19321 tcagtttgaa gctttgttag aaattacaac attaccagaa ggtagaattt atgttgctcg
    19381 ccaagataaa ctcattgtgg gttatgtcac tttccactat cctgatgaaa ttgagcgctg
    19441 gtctacaggt aaccttccat atttaatcca attgggggca attgaagtca gcatcaattt
    19501 taggcaatta caacttgcag aaaaattaat acaacttagc ctttctacac cagaattcga
    19561 ggattatatc gttataacta cagaatatta ctggcattgg gatttaaaaa attcaaagtt
    19621 agatgtattt gactataaaa aattaatgca gcggttaatg gcaactggtg gacttgaaat
    19681 attcgctaca gatgatccag aaataacaag taatccagct aattgtttaa tggcaagaat
    19741 tggcaaaaat attacattag aacagcaaca agcgtttgat gatattcgtt atatgaatcg
    19801 gtttttcttt taatttttgg aatatcggag ggattatatg caacaacatt tatcaaaaac
    19861 tgcatatgta tattcagata agttattaca atatcgattt catgaccaac atcccttcaa
    19921 tcaaatgcgt ttaaaattaa caacagagct acttttgaat gcaaatttat tgtctccaga
    19981 acaaatagta caacctagaa ttgcaacaga tgacgaatta atgttaattc ataaatatga
    20041 ttacgtcgaa gctattaagc atgcttcaca tggcattatc agtgaagatg aggctatgaa
    20101 atatggatta aatgatgaag agaatggtca atttaagcat atgcaccgcc atagtgccac
    20161 aattgttgga ggcgctttaa ctttagcaga tcttattatg tcaggcaaag cattaaatgg
    20221 ttgtcactta ggtggtggtt tgcatcacgc tcaacctggt cgagcaagtg gtttttgtat
    20281 atacaatgat attgcaatta ctgcacaata cttagctaaa gaatacaatc aacgcgtttt
    20341 aatcatagat accgatgcac atcatgggga tggtacacaa tggagtttct atgctgataa
    20401 ccatgttact acttattcta tccatgaaac cggaaagttt cttttcccag gctctggtca
    20461 ctatactgag cgcggtgaag atatcggcta tggacacact gtaaatgtcc cacttgaacc
    20521 gtataccgaa gattcatcat ttttggagtg ttttaaatta acagttgagc cagtcgtaaa
    20581 gagttttaaa cctgatatta ttctaagcgt aaatggtgtc gatatacatt atcgtgatcc
    20641 actaactcat ctaaattgta cgttacattc attatatgaa attccatatt ttgtaaaata
    20701 tttagctgat acttatacga atggaaagat aattatgttt ggtggcggag gctacaatat
    20761 ttggagagtt gtaccacgtg catggagtca tgtattctta agtttaattg atcaaccaat
    20821 tcaaagtggt tatttaccgt tagaatggat taataaatgg aaacattatt catctgaatt
    20881 attacctaaa agatgggaag atcgtttaaa tgattatacc tatgtccccc gcacaaaaga
    20941 aattagtgag aaaaataaaa aattagcttt gcatatagcg agttggtacg aatctactcg
    21001 tcaataagtc acctaatctg caacgatttt aaatgatgaa taaaaggcat tcttccaaca
    21061 cccgtataag tcgagtcgtt agatgaatgc ctaattttgt gaatttattt tgtagttcct
    21121 cggtattcaa ttctgtgtgg taaaactaca ttgggttctt ctatcttttc atcgttcata
    21181 tattttgtta ataagcgcat ccctactgca ccgatatcat ataatggctg aataacacta
    21241 gaaagttgtg gtctaaccat ctcaactaag cgtgtattat tgaaactaat aatttgtaat
    21301 tcctctggaa ctttaatacc agcatccatt gcactatgca tgataccaat agcttcttcg
    21361 tcactgatac ataatatagc atctggaagg ttacctttca ttttagcaaa agcttttacg
    21421 ccttctttat aactttcagc accagaacaa ttcaatgtat cacctaattg aagactattt
    21481 ttatttaaca cttcagttaa acctgctaat acatcttctt gagctttttt agaatgttct
    21541 ccacctacta aagcaaatga tttagcgcct ttttcaatta attttcccgt aatttctttc
    21601 gcagcttcag taaaatcaat attaacagat gctatatgtg catccttacc atttgttcct
    21661 gatactacta caggtacaga tgattgattt atcaattctt tcatttcttc agtaattgta
    21721 ccaccaagga aaataatacc atcaacttgt ttacttaata agttattaaa aatttctttt
    21781 tctttttcag gatcgttatc tgaatttgaa ataattgagt gatatttata cattgttgca
    21841 atatcttcaa gtccacgagc aagttgtgaa taatagatat tagatatatc tggaatgatc
    21901 acacctactg ttgttgtctt tttactagct aaacctctag caacagcatt tggacgataa
    21961 ttcaaacgct taatgacttc gttaacttta tttttagttt ctgctttaac attttggttc
    22021 ccattaacaa cacgcgacac tgtggccata gagacacgcg cttctcttgc tacatcatat
    22081 atagtaactg tcataatttc ctccttgtaa acgttttatt cattataata acatgttttc
    22141 atttcatttt catagtttac catattttaa tttacagtaa catatttttg tttggaaaaa
    22201 tttagcgaaa agcctgaaat aaaaacaaac gctaaatgat aacgaacgat atattttaat
    22261 tttatataaa cttttttcat ttttaattgt aatattttga gaaagccttt tcaaatatca
    22321 attgcatttg ttgaaaaatg ttcacatttc agtaacaaat atgtgaatct attatatttt
    22381 acagataatt catagttata attttaatta ctactatcag tcataatctt taataattgt
    22441 gaaattgaac aagtgtcgaa gtattataac aatcgttaag tgcagtatac ctttatttct
    22501 atacaaaaaa taatagccac tctaaaagta atatactctt agaatggcta caagtttaaa
    22561 attgtcgatt caaagataat attctacttc aacatgactt attagttaag tagtcaatag
    22621 tttccttgga atattatttt aactttttag cgttatataa atcagacaaa ggctttaatt
    22681 catcataaaa tgcttggaat tcatctaaat ccatttgttg acccgcatca ctaagtgcaa
    22741 cagatggatc tggatgcacc tcagccataa cgccatcagc acctactgct aatgctgctt
    22801 tcgcagttgg taacatgata tctttacgac ctgtactatg cgtaacatct accatgactg
    22861 gtaagtgtgt accttgtttt aaaattggta ctgctgaaat atctaaagtg ttacgtgtcg
    22921 ccttttcata agttcggatt ccacgttcac ataaaataat gttttgatta ccttgtgaag
    22981 caatgtattc agctgcataa acaaactctt cgattgtagc agataaacca cgttttaata
    23041 gaataggctt tttcgtacgg ccagcttctt ttaataactc gaagttttgc atattacgtg
    23101 caccaatttg gaatacgtct aaatactcat cagccacttc aaaatcattt ggatttacga
    23161 tttcgctgac aacatttaaa tcatatttat ctttaatttg tttaagtatt ttaagacctt
    23221 cgacacctaa accttggaag tcatatggtg atgtacgtgg cttaaatgca ccgccacgaa
    23281 taaatttttc acctttagca tgtaagtttt tagcaacagc ttcaacttgt tcaaatgatt
    23341 caactgaaca tggcccaaat acaaatgatt tattgccgtc tccaataatg cccccattat
    23401 caaatgttac aatcgtatct tcaggtttca acttacgtga tacatataaa tgtttttcat
    23461 tttcagattt ttgtaaatct gtagaggctt tgaaaatttc tttaaataat tgcttaatag
    23521 tattatcgtt gaatggtcct ttgttactat cgattaagtc gttaagcatt tctttttcgc
    23581 gttgtggatc atagatacgt gtaccttgtt ttaatttttc ttccccaatt ttttgtgcta
    23641 gttcaccacg tttagataat aagtctaaaa tttgatgatt cagtgagaca atctcacttc
    23701 tgtatgattc taatttatta ctcatcctta ctcacctcta cgaattattt caagtatatt
    23761 ccactttaaa gcaataaaat ataatgttaa aaaatattct aacaatatca tatgtatagt
    23821 tcaaccctaa attgacagaa aataattaat tttctaacta tatgatgctt gactgacatt
    23881 tcgattttct cagtcattgt tggaaagtta tattctatat tgatatttat tagcacatgt
    23941 taatcaatta aataagtgtc atgattatag cttattttga gtcaacatta cagcttttaa
    24001 gaaaaatttt ggtttctatc ttctattata aatctaaaat tctcatacat tcaacctaac
    24061 gcttaataaa aattaacaac ctttgtatat taatataaat ctatagtgaa catcactcac
    24121 caacatattc agccatattt gaattagtga acgtcattgt acctacttag attcattatt
    24181 tatttgatta tcaaatattt ttccatcatc agcttaaaat gtgcaatatc actattgtta
    24241 tctaaagatg tttcaaacaa cataaataca tgaaatgatc gcgttgcctt tcaaaataaa
    24301 ctgatgatat caaaaaaggt aagcacctga atgcctacct tttattttga aattcaattt
    24361 ttaaaaatat aatacataga tgactgctat gattgattat taaacgattt tagtatgctt
    24421 agtcattaaa tgtacgtttc tctattttac ttttagcttt ttcaacttta gcatttgatt
    24481 tttttgatgc attggatgat ttagcatttg ttgcttttga atttttttga ttagtctttg
    24541 tcgttgagtt acttgaacta ctttgtttct ttgcaccttg tgatgctttc ttattatgtt
    24601 gctttttctg acctgaagtt ttatttgtcg atgcttttga tgcatttcgt ttatttgaag
    24661 gtgttgtttt tttagattgt ttacctgatt tcttgtcaac agctgtatta ttaggtgttt
    24721 tttcatcagc tttacgtgta ataacaccat tttcaaattt agcttctttt tgtttaacag
    24781 tgtctttatt atctgtatta actgtttctg atacatcatt ctttttagta actaatagag
    24841 gaatttcttt caagtcattt ttagcaactg gtttttctgc aaataacgct tcagttaatt
    24901 gactctcttt tgagcctggt gttaatttag cttgcttctg ttttgcagcg tttgctaatc
    24961 tttcagcttg tgactcttct ttcgatacgt ttaatgtcga tgcttttgat tcttcatttg
    25021 atacatctgc acttttatct gtttctgctt gcgcttcttt cttcgcttct tgaatcgctt
    25081 gtgcttcttg tgatgtatca cttaaattat ttgcacttgc ttcttctttt atcgctgctt
    25141 gttgtgcctt caatgccgat gcctttgact cttcatttga tacagccgca cttttatccg
    25201 tttctgtttg tgcttctctt ttagcttctt gaatctcttg tgcctcttgt gatgtatcgc
    25261 ttaaattatt tgcacttgct tcttctttta ttgctgcttg ttgtgccttc aatgccgctt
    25321 gttcattttt agatttgttt aaaaatcctt caacacgttc ttttgtaaag gcaaccgttt
    25381 cttcaagttg cgtttttctt tcttcaaact tttgcgacag ttcttgttct ttgactttta
    25441 aatcatctgc tttttgataa actttatttt taaaatataa gcctaaagct gaaccaacaa
    25501 gcgcgccagt tataaaacta acaacaaaat ctttacggtt tggtaatggt tcattttgat
    25561 aagtgtgttt attttttaat gttcttttat tattttgttg ttgcgtcatt tgtatatcct
    25621 cctataacaa atgcacttaa ataagtaaca acctcattta agtgcatttg tttaattatt
    25681 aattatttat ctactcgaga agtatagcta tgatttgcat cagttgctac attattagct
    25741 ttgtaatttg cacttccacg acggtagtgt ctattttgcc atttgtctgc aatttccatt
    25801 gcaacatttg accattgaac aacttgtgag attttatctt cattttgaga aatattatgt
    25861 gtaattgaat ttgttacacg atctacagag ctgtttaacg tttgtactga gtcaccgata
    25921 cctttaacag catctactac tgagtttaaa cgatctactt taccttggat atcctcagtt
    25981 aaacggttta ctttatgaag taaatctgtt gtttcacgag taataccttg aacttgacct
    26041 tctacaccgt caagtgtttt tgcaacataa tctaagtttt tcttaacaga atttaataca
    26101 gctacgatac cgatacataa aattaagaat gcaatcgcag cgataattcc agcaattggt
    26161 aaaatccaat ccattaaaaa cgcctcctaa ttaacatgta ataatgtcat taataataaa
    26221 tacccatact actctattat aaacatatta aaacgcattt ttcatgccta atttatctaa
    26281 atatgcattt tgtaattttt gaatatcacc tgcacccata aataaaataa cagcattatc
    26341 aaattgttct aatacattaa tagaatcttc attaattaac gatgcacctt caattttatc
    26401 aattaaatct tgtatcgtta atgcgccagt attttctcta attgatccaa aaatttcaca
    26461 taagaataca cgatctgctt tacttaaact ttctgcaaat tcatttaaaa atgcttgtgt
    26521 tctagagaaa gtgtgtggtt gaaatactgc aacaacttct ttatgtggat atttctttcg
    26581 tgctgtttca attgtagcac taatctctct tggatggtgt gcataatcat ctacaataac
    26641 ttgatttgca attgtagttt cattgaaacg acgtttaaca ccaccaaacg tttctaatgc
    26701 ttctttaata tttgtaacat ctagcttctc taaataacta atcgcaatta cagctaatgc
    26761 atttaaaact gtatggtcac catattgtgg agataggaag tgatcataaa actcaccatc
    26821 cacatacaca tcaaaagcag tacctttatc cgtaatttga atattttgag cataaatgtc
    26881 atccgactct ttaaatccat agtaataaat tggaacatct gcttcaattt tacgtagatg
    26941 ttcatcatca ccccaagcaa taataccttt tttaacatta tgtgccattt cttggaatgc
    27001 atcaaaaaca tcattaatat ctttaaaata atcaggatga tcgaaatcaa tatttgtcat
    27061 aattgcgtaa tcaggtttat aacttaaaaa gtgacgtcta tattcacatg cctcaaaagc
    27121 gaaataatca ctttcaggca atcccatacc tgtgccatca ccaattaaaa atgaagtctt
    27181 tttatcacca ttcataacat gtgataataa acctgttgta gaagttttac catgtgcacc
    27241 agttacagct actgaagtat attgatcaat aatctgtcct aaaaaatcat tataacttac
    27301 aacatctaat ttcaattgat gtgcacgtac tatttcttca tggctactcg cgaatgcatt
    27361 accttgtata actaccatat cttcttttat gttattagca tcaaatggta atatttttat
    27421 ccccttattt ctaagagcaa cttctgtaaa tacgtagttc tcaatatccg atccttgaac
    27481 ttcatgtcct aaatcatgca tgatttgtgc taatgaactc atgccagaac ctttaattcc
    27541 gacaaaatga tagtgtgtca ttatataaaa ctccttactc ataattattc tttatttaaa
    27601 tctgcttccg taacataaac atcccttggt tttgaaccat tagcactcga aacataaccg
    27661 agttgctcta attgatcgat aattcttgct gctctattat agccaatttg gaaatgtctt
    27721 tggattaatg atgttgaaat atgtccttca ttaaccataa atgcacaaac atcatcaaat
    27781 aattcatctt gtgattgtgt ttgtgttttt ttcaacaatt ctttttcttc aaataggtag
    27841 tccggttctc tttgttgttt gataaaatca acaacatcat caatttcgtc atcagaaaca
    27901 aatgtacctt gaactctaat cggtttattc ataccgctac caagatataa catatcgcca
    27961 tatcctaaca agcgttctgc tccaccactg tctaatatcg ttcttgaatc tacacttgat
    28021 gataccataa atgcaattct tgttggtatg ttggctttaa ttaaacctgt aattacattg
    28081 acagatggtc tttgcgtagc taccaacata tgaataccac atgctctcgc tttttgagca
    28141 attctagcaa tagactgctc aacttcttgc ggagccatca tcattaaatc agccaactca
    28201 tcaattacta tgacaatttt tggcattctt tcatcatatg gtgctttttt gttaaatgct
    28261 gttatattac gtacatggta atgtgcaaat aacttataac gtctttccat ttcttctacg
    28321 gcccatttta aactctgtgt agctgctttg acatctgtaa ttaccggtgc aactaaatgt
    28381 ggcaaaccat tataaggagc taattcaacc atttttggat caataagtaa taatcttaat
    28441 tcctcaggat gatttttata tagtaaagac atcaaaatac tattgataca aactgatttc
    28501 cctgatccag ttgcacctgc aattagtgcg tgtggcgttt tagcaatatc cataagtaat
    28561 ggttcattat taattctata ccccatcgca actgttaatt tagattcagc atttttaaaa
    28621 cttggagatt caataataga acgtaagttg accgtcgttg gattttggtt cggaacttca
    28681 ataccaacac gactagttcc tggaattggc gcttctatac gaatatcttt cgctgccaat
    28741 gccattttaa tgtcatcttg taatgccgta attcttgaaa ctttaacacc tttttcaact
    28801 gataattcaa atcttgtaac acttggacct tcagttacat cttgtacttc tgcaggtaca
    28861 ttaaagtaaa ataatgcgtc attaagttct ttctttttat ctgtaatcca gtcctcgtcc
    28921 ggctcaataa cttgtggttc ttctagtaat gaaacacttg gcaatttaat atttgggcct
    28981 ttacgaatca tcggcttaga agtttgtaca gcttgatttg ttgcaacatc tggttgattc
    29041 gtccgaggtc tattttcatt agctgtatct tcattttgat ttgaaaatga aagctgtaaa
    29101 tctttttgtt gatcatcatt atcttgttgt ccactagtat tgttttgatg tgttgtttct
    29161 tcgctttctt cagttatgtc gcttacttct gaaactgaag acgaagttga ttgatcaact
    29221 tgctcatttt ccaattgatt atcatcttgg ctttcttcaa atatttccgt ttgagttgaa
    29281 tcagcagaag gtttctgctc tgaatattgt tgtgcatttt gataatcatt ttctgttgct
    29341 gcatgaccaa taagttgatt gttctcaaca ttgtttgatg tcatattatt tgttttatat
    29401 gcattcgtgt ttgactcttg tgaatcagta cttgatgatg gtgtgttttg agtcgcagtc
    29461 ttgctttcac tcgcagcttg tttactttgt acaggcttta attcaggcac attgactttt
    29521 gaatgctttt tacgatccat catacgcttt ttatcagatg gcgtcatgac aacattaaat
    29581 ggtcttttac ttaaagttga aaccttttca gtctgtttct ttggatttac gttttcaata
    29641 gttttctctt cgacattttg tttactttca gctgcttcgt tttgcacatt ttctgtaa
//
LOCUS       DAMZPP010000012        29472 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMZPP010000012 DAMZPP010000000
VERSION     DAMZPP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 29472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29472
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.012"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..3426
                     /locus_tag="POH73_000428"
     CDS             <1..3426
                     /locus_tag="POH73_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDI6886406.1"
                     /translation="MSARMHKGTEQMLGMFANTLVYRGQPSLDKMWTQFLQEVKEMSL
                     EAYEHQEYPFECLVNDLDQSHDASRNPLFDVMLVLQNNETNHAHFGHSKLTHIQPKSV
                     TAKFDLSFIIEEDCDDYTINIEYNTDLYHSETVRHMGNQCMIMIDYILKHQDTLQIRD
                     IPNGTEELLNWVNTHVNDRMLNVPGNKSIISYFDEVVSRQGNHVALVMNDLTMTYETL
                     HNYVDAIAHMLLSNGVGKGQPVALITERSFEMIAAMLATVKVGASYIPIDIDFPKKRQ
                     GAILEDAKVTAVMSYGVEIETTLPVIQLENAKGFVESKENEQYDDLHGDQLENTAMLD
                     NEMYAIYTSGTTGMPKGVAIRQRNLLNLVHAWSTELQLGDNEVFLQHANIVFDASVME
                     IYCCLLNGHTLVIPDREERVNPEQLQQLINKHRVTVASIPLQMCSVMEDFYIEKLITG
                     GATSTASFVKYIEKHCGTYFNAYGPSESTVITSYWSHQCGDLIPETIPIGKPLSNIQV
                     YIMSDGLLCGIGMPGELCIAGDSLAIGYINRPELMADKWQNNPFGKGKLYHSGDLARY
                     TSDGQIEFLGRIDKQVKVNGYRIELDEIENAILAIRGISDCVVTVSHFDTHDILNAYY
                     VGEQQMEQDLKQYLNDHLPKYMIPKTITYIDCIPLTTNDKVDTTRLPNPSPIQQSNKV
                     YSEPSNEIEQTFVDVFGEVLKQNDVGVDDDFFELGGNSLEAMLVVSHLKRFGHHISMQ
                     TLYQYKTVRQIVNYMHQNQQSLVALPDNLSELQKIVMSRYNLGILEDSLSHRPLGKTL
                     LTGATGFLGAYLIEALQGYSHRIYCFIRADNEEIAWYKLMTNLNDYFSEETVEMMLSN
                     IEVIVGDFECMDDVVLPENMDTIIHAGARTDHFGDDDEFEKVNVQGTVDVIRLAQQHH
                     ARLIYVSTISVGTYFDIDTEDVTFSEADVYKGQLLTSPYTRSKFYSELKVLEAVKNGL
                     DGRIVRVGNLTSPYNGRWHMRNIKTNRFSMVMNDLLQLDCIGVSLAEMPVDFSFVDTT
                     ARQIVALAQVNTPQIIYHVLSPNKMPVKSLLECVKCKEIELVSDESFNEILQKQDMYE
                     TIGLTSVDREQQLAMIDTKLTLKIMDHISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            3439..4083
                     /locus_tag="POH73_000429"
     CDS             3439..4083
                     /locus_tag="POH73_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDI6886407.1"
                     /translation="MKVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(4412..4891)
                     /locus_tag="POH73_000430"
     CDS             complement(4412..4891)
                     /locus_tag="POH73_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDI6886408.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMQLPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(5176..5946)
                     /gene="argB"
                     /locus_tag="POH73_000431"
     CDS             complement(5176..5946)
                     /gene="argB"
                     /locus_tag="POH73_000431"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDI6886409.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQQQCSAIGLCGLD
                     AQLFEIKRFDQQYGYVGVPTTLNIDSLSYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIAASLEAPIYVLSNIAGVLINDVVIPQLPLADINQYIEHGDIYGGMIPKVLDAKN
                     AIKNGCPKVIIASGNKPNIIEAIYNNDFVGTTILKSSV"
     gene            complement(5962..7203)
                     /gene="argJ"
                     /locus_tag="POH73_000432"
     CDS             complement(5962..7203)
                     /gene="argJ"
                     /locus_tag="POH73_000432"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDI6886410.1"
                     /translation="MKHQETTSQQHNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASSAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DAQQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHINDANFATPGAFNEA
                     ILTTDTCTKHIAVSLNIDGKTITIGGSAKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANQQVEHQILNQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNILTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            complement(7215..8246)
                     /locus_tag="POH73_000433"
     CDS             complement(7215..8246)
                     /locus_tag="POH73_000433"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDI6886411.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSLDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMARGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLKQSPVYP"
     gene            complement(8285..9469)
                     /gene="rocD"
                     /locus_tag="POH73_000434"
     CDS             complement(8285..9469)
                     /gene="rocD"
                     /locus_tag="POH73_000434"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDI6886412.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVITEGKGGKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSDVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKSGFHP
                     LLQGTKTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVEQLIKRGILCKDTHRTIIRLSPPLVIDKEEINQIVAAFQDVF
                     KN"
     gene            complement(9722..11077)
                     /gene="brnQ"
                     /locus_tag="POH73_000435"
     CDS             complement(9722..11077)
                     /gene="brnQ"
                     /locus_tag="POH73_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDI6886413.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSNALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKIIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            complement(11355..11912)
                     /locus_tag="POH73_000436"
     CDS             complement(11355..11912)
                     /locus_tag="POH73_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HDI6886414.1"
                     /translation="MINFDKTALVLIDLQQGILKMDYAPHTAENVVQNANKLIEAFRK
                     NNGFIAFVRVNFYDGKDALQPNAMISLPPKEGDDYSHFHYLLDKRDDDFVIDKRQFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHVGVDTTARDAYQLNYDQYFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDLLNILN"
     gene            complement(11979..13619)
                     /locus_tag="POH73_000437"
     CDS             complement(11979..13619)
                     /locus_tag="POH73_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HDI6886415.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQAGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENAATEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTAAKDTDASTYIELLVSKLHQSKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLSSLLQQLTDISYTNN
                     ASFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     SNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQEVF
                     NTINGHPDVMHFVEVKMSVEDAPKKLIDIAKAFSQQNK"
     gene            13811..13912
                     /locus_tag="POH73_000438"
     CDS             13811..13912
                     /locus_tag="POH73_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886416.1"
                     /translation="MMLQNTIAMKKQARHLMKVYAPVYSKHYLASNN"
     gene            complement(13891..15936)
                     /locus_tag="POH73_000439"
     CDS             complement(13891..15936)
                     /locus_tag="POH73_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HDI6886417.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGEALQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQTGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            16521..17576
                     /locus_tag="POH73_000440"
     CDS             16521..17576
                     /locus_tag="POH73_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDI6886418.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYEHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSGLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     IRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDSVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            17576..18472
                     /gene="murQ"
                     /locus_tag="POH73_000441"
     CDS             17576..18472
                     /gene="murQ"
                     /locus_tag="POH73_000441"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDI6886419.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            18484..19938
                     /locus_tag="POH73_000442"
     CDS             18484..19938
                     /locus_tag="POH73_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI6886420.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHNHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            19941..20816
                     /locus_tag="POH73_000443"
     CDS             19941..20816
                     /locus_tag="POH73_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI6886421.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(21011..21292)
                     /locus_tag="POH73_000444"
     CDS             complement(21011..21292)
                     /locus_tag="POH73_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886422.1"
                     /translation="MVLITYQIILFLIISLSYYLTLNHFMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIITFSTFVLVHLALKLFFNI"
     gene            21505..24294
                     /locus_tag="POH73_000445"
     CDS             21505..24294
                     /locus_tag="POH73_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDI6886423.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAHLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTVAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFI
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            24494..25357
                     /locus_tag="POH73_000446"
     CDS             24494..25357
                     /locus_tag="POH73_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HDI6886424.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTMLNKLLLGLHSVNTTTALSEMPIGNSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVL
                     TSRNTLSEVQKPKENNSLLNSKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMIFELKKRGNMKEYKDEFLAAPTEIKFLLYFAKMTKKDKNKLYHF
                     LKDTNNPPHFT"
     gene            complement(25562..27154)
                     /locus_tag="POH73_000447"
     CDS             complement(25562..27154)
                     /locus_tag="POH73_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6886425.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLTNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVLFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTIVSLLEKVGLDQSFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            27111..28589
                     /locus_tag="POH73_000448"
     CDS             27111..28589
                     /locus_tag="POH73_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6886426.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VISIITYLESFKSLYHKAFYKFVLSVLSLPLFLYAVIKFFFSAKRKNFYANNSEISEI
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKTQSSNTNSKNIEQAHFSTYFENVLFHKFI
                     IIKVILALPMFILLTIYLQPLVRYIFERIVMAVIVIIGVIVSVFSILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNDVVTKAVGERIP
                     ITIIVAVLALIVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            28595..>29472
                     /locus_tag="POH73_000449"
     CDS             28595..>29472
                     /locus_tag="POH73_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6886427.1"
                     /translation="MVKLTTKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVVFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTW"
BASE COUNT         9801 a         4673 c         5544 g         9454 t
ORIGIN      
        1 atgagtgcgc gtatgcataa aggcacggag caaatgctag gcatgtttgc taatacgttg
       61 gtatatagag ggcaaccatc acttgataaa atgtggacac agtttttaca agaggttaag
      121 gaaatgagtt tggaggcata cgagcatcaa gaatacccat tcgaatgttt agtaaatgac
      181 ttagatcaat cacatgatgc ctcacggaat ccattatttg atgtcatgtt agtactacaa
      241 aacaatgaaa cgaatcatgc tcattttggg catagtaaat taacacacat tcaacccaaa
      301 tcggtgacgg cgaaatttga tttatctttc atcattgaag aagattgcga tgactataca
      361 atcaatatcg agtataatac cgatttatat cactcagaaa cagttcgtca catgggtaat
      421 caatgtatga ttatgattga ttatattttg aagcatcaag atacactaca aattcgtgat
      481 ataccaaacg gcacggagga acttttaaat tgggtcaata cgcatgttaa cgatcgaatg
      541 cttaatgtcc cgggaaataa atctatcata agttactttg atgaagttgt ctcacgacaa
      601 ggtaatcatg ttgcgctagt catgaatgat ttgacaatga cgtatgaaac attacacaac
      661 tatgtagatg ctattgcgca catgctccta tcaaatggtg tgggcaaggg tcaaccagtt
      721 gctttaataa cagaacgtag ttttgaaatg attgcggcga tgttggcgac agttaaagta
      781 ggtgcatctt atatacctat cgatattgat tttccgaaaa agcgacaagg tgcaattttg
      841 gaggatgcta aagtaactgc agtcatgtct tacggcgttg aaattgaaac gacattacca
      901 gtcattcaat tggaaaatgc taaaggtttt gttgaatcaa aggaaaatga acaatatgat
      961 gatttacatg gcgatcaact cgaaaacaca gcgatgttag ataatgagat gtatgctatt
     1021 tacacatctg gtacgaccgg gatgcctaaa ggggttgcca tacgacaacg aaatttgttg
     1081 aatttagtgc atgcatggtc aactgaattg caattaggcg acaatgaagt atttttgcaa
     1141 catgcaaata ttgtttttga tgcatcagtt atggagattt attgttgttt gttaaatggt
     1201 catacgcttg tgattccaga tagagaggaa cgtgttaatc cagaacagtt acaacaactc
     1261 attaataagc atcgtgtgac ggtggcgtcg attccattac agatgtgtag tgttatggaa
     1321 gacttttata ttgaaaaatt gattacaggc ggggcaacta gtacggcatc ctttgttaaa
     1381 tatattgaga agcattgtgg cacgtatttc aatgcctatg gaccatctga gtcaacagtc
     1441 atcacatcgt attggtcaca tcaatgtggt gatttgatac ctgagacgat tccaattggc
     1501 aaacccttat ctaacatcca agtgtatatt atgtcagatg gtttgttatg cggtattggt
     1561 atgccaggcg agttgtgtat tgcaggtgat agtttagcga taggatatat taatcgtccg
     1621 gaattaatgg ctgataaatg gcaaaataat ccatttggta aaggaaagtt gtatcatagt
     1681 ggtgatttag cacgttatac atctgatggt caaattgaat ttttgggaag aatagataaa
     1741 caagtgaaag ttaacgggta ccgtattgaa cttgatgaaa ttgaaaatgc aatattagct
     1801 attcgtggta tatctgattg tgttgtcaca gttagtcact ttgatacgca tgatatattg
     1861 aatgcttatt atgtcggaga acaacaaatg gagcaggatt tgaagcaata tttaaatgat
     1921 catctgccta agtatatgat tcctaagact ataacgtata tcgattgtat accattaacc
     1981 acgaatgaca aggtggatac tacgcgtttg ccaaatccat cacctataca acagtctaat
     2041 aaagtatata gcgaaccatc taatgaaatt gagcagacat ttgttgatgt attcggagag
     2101 gtattgaaac aaaatgatgt cggtgttgac gatgatttct ttgaacttgg tggtaactca
     2161 ttagaggcga tgttagttgt ctcgcattta aaacgatttg gccatcatat ttcaatgcag
     2221 acattatatc aatataaaac cgtgcgacag attgttaatt atatgcacca aaatcaacaa
     2281 tcattagttg cattaccgga taatctttcg gaattacaaa agattgttat gtctcgttat
     2341 aatttgggta ttttagagga tagtctaagt catcgacctc taggaaaaac actattgact
     2401 ggcgctacag ggtttttagg tgcttatctg attgaagcac tacaaggata cagtcatcgc
     2461 atttattgtt tcatacgtgc tgataatgag gaaatagcat ggtataagtt gatgacgaat
     2521 ttaaatgatt atttttcaga agagacggtt gaaatgatgt tatcaaacat tgaagtcatt
     2581 gttggtgatt tcgagtgtat ggatgatgtt gttttaccag aaaacatgga tacaattatt
     2641 catgcaggtg ctcgtacaga tcactttggt gatgatgatg aatttgaaaa agtaaatgtt
     2701 caaggtactg ttgatgtcat acgtttggca caacaacatc atgcaaggtt aatatatgtg
     2761 tctacgataa gtgtgggaac ttattttgat atagatacag aagatgtgac attttcagaa
     2821 gcggatgtct ataaaggaca actactaaca tcaccatata cacggagtaa attttatagt
     2881 gaattaaaag tattagaagc tgtaaaaaat ggcttagatg gtcgaattgt acgtgttggt
     2941 aatttgacga gtccatacaa tggaagatgg catatgagaa atataaagac taaccgtttt
     3001 tcaatggtaa tgaatgattt gttacaactg gattgtatcg gggttagcct cgctgaaatg
     3061 cctgtagatt tttcttttgt ggatacgact gcaagacaaa ttgtcgcatt agcacaggtc
     3121 aacacgccac aaatcattta ccatgtgtta tcacctaata aaatgccggt gaaatcttta
     3181 ttagaatgtg ttaagtgcaa agaaattgaa ctcgtcagcg atgaatcatt taatgaaatt
     3241 ttacagaaac aagacatgta cgaaacgatt ggattaacta gtgttgaccg tgaacaacaa
     3301 ctagcaatga tagatacaaa attaacatta aaaataatgg atcacatcag tgaaaaatgg
     3361 ccaacgataa ctaacaattg gctgtatcat tgggcacaat atatcaaaac aatattcaat
     3421 aagtaagtag ggaaagttat gaaagtattt gtaatgcaat tacagagtaa cttgaaaagt
     3481 attgaagaat taatatcaca aagtcgttgg tcatataaaa aaccgcgtac agtcaactat
     3541 agatacaatc aagataaact catgcacaga ttgggagata ttttagtgca atatggaatt
     3601 caacatgaca cagggttatt accacatgaa tggcattatc acatttcgcc acgaggtaag
     3661 gcagatatag ttcaacacaa tcgtgatgga cagcccatct atgtgagctt atcatatagt
     3721 tatccttata tcgtgtgtgt tgtcgataaa gaaccagttg gtattgatat cgaaaagata
     3781 tcacaacgtt tagactggcg tacattagtg acgtgtttct ctacaaacga agcacatcaa
     3841 atatgtagtt taaatgattt ttatcaaata tggacacaaa aagaaagttt tacaaaattg
     3901 attggtgaag gtttaatcaa aggattggac atttatgata tgacgcaatc acacttttat
     3961 caatcacgtg aagtgaagtt caaacaattt atttttgatc agtttatggt acaggtatgt
     4021 ttcttaggac aggcaccctg gggttataaa aaagtgtctg tatttcagtt attgagtagt
     4081 taaacatggc gtctaacagt tttatttggc agtgatttta aactatactg cctaaatgtt
     4141 tactgttaga tgttttttta ttggaaaaat gaaaggtgaa tacacgatgg catcattcaa
     4201 aatcatgacg agattaaagt aagtgataag gtgaaaatat actgagcaat attgaaattg
     4261 ccaaaaataa tggcttgtta cgacgcattt gagactttaa tgctcatgta agttggctag
     4321 aattatatca tgtcagttaa taaaataacg tgagcatcaa tcatcatact caaacgatga
     4381 tttaaaactc acgttaatgt tagtcagtgt attaatttaa tttatagcga tagacaaaat
     4441 gttgtcgcac taattcaatt gtcatcatcc agacgatatg gccaaagaat tctgacagat
     4501 gctcttggaa tggttgatcc cacacagcag gtacagtatg catgattggc ataatgataa
     4561 ggtggaataa tacccaaata gcaataccaa aaacagcacc ttgtcccatt gctaagtaag
     4621 cgtatttttt aactaatatg cagtaaataa ttgcaatgac gatagaaaaa ctaaagtgga
     4681 caataaagct tacccaaggc aattgcatat ttgaaaatgt atatgtttga tgcgtaaact
     4741 cactactaaa tcctaattgt tgcaataact cttgaggtgg gttcgttgca ttacgttctg
     4801 gtgtgcgagg tggaaacatg acctcccaac ctaattttac aattccagat aacaagccac
     4861 cgataattcc agcataaatg aatataccca tttgtcgttt tgtcaactta aggactcctc
     4921 tctatttact atcctcatta tagtattttg tgaaaataat cacaatgaaa aacttttaaa
     4981 atataaaata tttcgagtta caaattttaa aatcacttat agtgtttttc atctcatatt
     5041 gtatcatact gtattctaag aattcttttg tgaaatgaga caggtgaaaa ggtgacgctg
     5101 cataatcatt aaacaaatta ggtacaacta tgtttcgaag ccgaaaaata caagattgag
     5161 tggtcatatt caaagtcata ctgatgattt tagtatcgtt gtcccaacaa aatcattatt
     5221 gtaaatagct tcgataatat ttggcttgtt tcctgatgca atgataactt taggacagcc
     5281 atttttaata gcatttttag catcaagcac tttgggaatc atacctccat aaatatcacc
     5341 atgctcaata tactgattaa tatcggctaa gggtagttga ggtataacaa catcgttgat
     5401 tagcacgcca gcaatattac ttagtacata aatgggtgct tctaatgatg ctgcaataaa
     5461 ataggcgagt gtgtctgcat ttatgttgta aaattctcca tcatggttat tgaaaccaat
     5521 cgaattgatg ataggtacaa atttagtaca taaataagac aatgagtcta tatttaaagt
     5581 ggttggaacc ccgacatatc catattgttg atcaaaacgt ttaatttcaa ataattgagc
     5641 atccaaacca cataagccta ttgcagaaca ttgctgctgg ttaaattgag ctactaatgc
     5701 agtgttaacg tctgcaatga gcgtgtgttt agtaatagtc atggttgctt tatcagtcac
     5761 tcttaggcca ttaacaaagt gtggctcgat ttgctggttt gataatgctt cattaataaa
     5821 tgggccaccg ccgtgaacga taatggggta gatgttgttt gatcgtaaat gcttaatgtt
     5881 gttaataatt gatggatgca tgtcacttag tgtactgcca ccaattttaa tgacaataaa
     5941 tttcatctaa ccaacaccac cttatgttcg atatgatgcg ttaatacgca cataatcata
     6001 ggataaatca caaccgtatg cagtcgctgc agcgttaccc aaaccaagct gaacgtcaat
     6061 tgtgacattt tcatgagtta atatattcga catagcttgc tcatcaaata gtacagccat
     6121 acctttatcc acgacaggta tttggttcag ttgaacatat gtgcagttag gatcaatttc
     6181 acatccgcta tagccaatag ctgtaatgat tcgaccaaaa ttggcatctt cgccaaaaat
     6241 agctgatttt actagatttg aacttacgat agttttaccg atttttcttg cgtctgatat
     6301 tgatttagcg cctgaaacat tgacactgat taactttgtt gcgccttcgc catctctggc
     6361 tatagcttta gctaaaaatg tacagacaaa attgaaggca tcaacaaatg tttcccattg
     6421 tggatggtct tgattaagta tttggtgttc aacttgttga tttgccatga ctaataccat
     6481 gtcatttgta cttgtatcgc catcaacagt aatcatatta aatgtatggt cagtcgaaga
     6541 ttttaataat tgatgaagtg tattcgattc aattgatgca tcggttgtta taaaagcaag
     6601 catggtagcc atatttgggt gaatcatacc tgatccttta gcactgccac caattgtaat
     6661 ggttttacca tcaatattta gtgatacagc gatatgcttt gtacatgtat cagttgttaa
     6721 aattgcctcg ttaaacgcac ctggcgttgc aaaattagca tcattaatat gttctgtacc
     6781 agttttaatt ttatccattg gcaaatattc tccaataaca ccagttgaag caacagcaac
     6841 atgctcagat ggtatttgaa gttgttgagc aacccatgtt tgagtttgtt gtgcatcatc
     6901 gataccttgc tgacctgtgc aagaatttgc attagctgaa ttaacaacaa gtgcttgtaa
     6961 tttcccttta gacttttgta aagtgtcttc agtgacaata agtggtgcag ctttaaattg
     7021 gtttaaagta tatacggcag ctgaacttgc taaagacgat gagtaaatcc agccaaagtc
     7081 ttttttgtta gcgcgtaaac cgatgtgcat accaccggcc gtgaagcctt gaggtgtact
     7141 gatatcgcca tgtttaataa ttgaaaagtt atgttgttgt gatgtcgttt cttgatgttt
     7201 cattctaaca ccccttatgg ataaactggt gattgtttta ggccagtcgt aacttcaaaa
     7261 tcatataata tatttaagtt ttgaatggct tgaccacttg cccctttgac aaggttatca
     7321 atcactgata ctaaaattgc tgtttgcgtt gtttcatcta catagatacc gatatcgcag
     7381 tagttactac cgagtacttc ttttgtggtt ggaaaagtcc caatatctct aattctgaca
     7441 aatggctgat tagcataata agaggtcatt aatttatgta atgattcagt cgtatattca
     7501 gatgataatt tgacatatat tgttgataaa atacctcgtg ccattggtac gagatgtggt
     7561 gtaaatatga ctgatacatc ttgacccgca atgatagata aatattgctc gatttccggt
     7621 ttgtgtttat ggtttccgat tgcataagcg cttagatttt cattcatttc tgaaaaatga
     7681 acacgttgtg ataatgaacg accagcacct gacacgccgg tcttagcatc aataataata
     7741 gatgacaaat ctacaatttt ttcgctaata agtggatgta atgctaataa cgttgctgta
     7801 gggaaacagc cagggttaga aatgagtttc gttccattgt tatctaaaga ttgccattct
     7861 gaaatgctgt aaatagcatg attcaaatca tcttgtgctg cagcagtttc tttgtaatat
     7921 gcttcatata tttcacggtt tttaattctg aatgcaccag ataaatcaat aacatgaatt
     7981 cctttttcta ctaaaggagg gatacatgtt ttactcacgg gtgctggtgt agcaaagaaa
     8041 attacatcac agtcattatt gtccactgta agtgcttcga aatgttgcat aatatgttgt
     8101 aaatgtggaa atgttaattt caacggctca tctacttttg aatgtgagta gatgtgtgca
     8161 atcgttacat gaggatgtgt ttgtaataat cgaattaatt caattgcgcc ataaccgcta
     8221 ccgccaacga tacctacttt aatcatcatg aatccctcct atatcatata taaatgatta
     8281 attgttaatt tttaaaaacg tcttgaaaag ctgcaacaat ttgattgatt tcctctttat
     8341 caatgactag aggtggtgac aatcgaatga tagtacgatg cgtgtctttg cataaaatgc
     8401 cacgtttaat cagttgttca acgaaaggtg cggcatctgt gttaagttcg atgccaataa
     8461 ataaaccacg acctctaatt tctttaatac taggatgttt aagttgtagc aacgctttta
     8521 ataaaaatga acctaagcgt tctgatcgtt caaccagttg ttcatcttta agtacatcaa
     8581 gcgctgccgt cgatattgca atggctaaag ggttaccacc aaatgttgag ccatgtgtac
     8641 ctggtgttag aactcgcatg acatcattat ttgcaagcac agcagataca gggtataagc
     8701 cgccacccaa tgccttacct aaaatataaa tgtctggaac gacttgctcc cattccatcg
     8761 caaaccattt cccagttcta ccaagaccaa cttgaatttc atctgcaatc aataatattt
     8821 gatgtttatc acatagttga cgcacagctt ggatatatcc tttcggtggt atattaacgc
     8881 caccttcacc ttgaattggt tctaaaataa ttgctgctgt atttggtgaa atagcttgtg
     8941 ttaattgctc aatatctcca aaatctactg ttttagtacc ttgaagtagg gggtgaaatc
     9001 ctgatttata tgcgtcgtgg ttagatagtg ataatgagcc aagtgtacga ccgtgaaaat
     9061 tgttattcat agcgatgatt tcaacttgtc cgtcagtgat gcctttaacg tcagagcccc
     9121 attttctagc aattttaatg gctgcttcaa cagcttcagt accagagtta agggggagta
     9181 ctttgtcttt cttagcaaga tgacaaattt tttcttccca tttcccgaga ttgtcactat
     9241 aaaggacacg tgaaatgata gacaactttg aagcttgttc tgtcatcgct ttaacaattg
     9301 ttggatgaca atggccttgg tttgcaactg aaaaacccga aatgcaatct atatattgtt
     9361 tgccatcagt atcccaaact ttgccccctt tcccttcagt aattacaagc ttcagtggtg
     9421 cataattatt agagctataa taatcagtta attcaatgat tgaattcatc ggtcgccctc
     9481 ctttatattt atacaattta aagaattaat attaatatat tacagttgtt caatataagt
     9541 gtcaaatgta aatttaataa tataaagata aagttgtgta tgcattaatg tgattcgtaa
     9601 aatgaaagta ctaatatttt tcgtggataa tcattaatat gtatgtatac gagtgcagtt
     9661 taatgtagta tgaataaaaa atgccacaag catatgttta acatatgact gtggcatagt
     9721 attatgcttg tggaatttta cgatgcttaa ttttataaat aatgaagccg ataatgaaac
     9781 caatcaaact gagaacaacc cagcccatac caatgtctga taatggtaaa tatttttggc
     9841 tgaaattaat caaagtttgt gagaatgatg tgcttgaaat gaactctgga ctagctttta
     9901 atccatctac taatgcagca atcattgtaa agaaaatggt acattgataa ataatttttg
     9961 aatgatggaa tttgctgcta aataatgtta gtacaatcag ggcaattgct aatggatata
    10021 agaacattaa cactgggact gagtacataa taattttagt taagccaaca ttcgcgaata
    10081 agaatgagat aaagctcaca actgttgcaa tcgctaggta attcatttta gggaaaaggt
    10141 gttcaaatgt ttctgaaaat gccgtaatta aaccgatagc tgtttttaaa caagcaacca
    10201 taacgatgag tgataacaag acgataccgt agttacctaa gtagtattgc gtaatttgcg
    10261 ctaaggcaat accaccattt tcactaagtt tgaaatgacc aatacttaat gtacccatga
    10321 ttgctagtag ggtataaatg atccccatca taatgatact gatagtacca gacttaattg
    10381 tttctttagc gatatcagtt ggattttcga tacctaactt tttaatcgtt gcaacaatga
    10441 tgataccaaa tgccaatgac gctagcgcat ctaaggtatt gtatccatct aaaaagccgt
    10501 taaataaggc gtttgattga tattgtttac taataggtgc atcagatatg ccacctaatg
    10561 gatggataaa agcaaataat aaaataattg ctaataatac taagaatacc ggatttaaaa
    10621 atttaccgat atattctaaa attcttgatg gctttctcgc aaaaaaccat gcaatcacaa
    10681 agaagacgaa gctgaaaata aataaatata aagtgatttg ctttggtgat aaaaatggcg
    10741 aaaatgcaat ttcaaatgat gtagttgcca gtctaggtaa ggcgaaaaat ggtccgataa
    10801 ctagatataa ggcaatcgtg aaaatataag catatgtttt attaacacgc gatgcaattt
    10861 caaataaacc agatgtcttt gaaataccaa tagcaatgat acctagaaat ggtaagccaa
    10921 ttgctgtaat taaaaatcct aagttagcga taaaaacgtt agaaccagca gcttgaccca
    10981 agtgtattgg gaagataagg ttgccggcac caaagaataa accaaataac atagaaccaa
    11041 taaacatgtt ttctttaaat gttagtttct tcttcatata tgtatttgtt ctccttttta
    11101 gaacttataa aaatctactt aattaagata atatcaaaaa ttaaacctaa agtgatacat
    11161 aattttgtaa taaatactaa attttttgaa aattccggaa taaatactgt tttttatgta
    11221 gtaagggcat aggttaagaa cggtttaaca gatatagaat atgtttatat agtggaggtt
    11281 agacaacccg acgtattaat acattgtgaa ataaagatac ccccttcaat ataatcgaag
    11341 ggggaagtga atgtttagtt caatatattt agcaagtcgt ttgtggttat ggtttgtccc
    11401 atcaatggga atacattatc tattgggaat tgatgtagcg tttcgttttg tgcactcatc
    11461 atatctgtaa caaaatactg atcgtagttt aattgatagg cgtctcgtgc tgtcgtatct
    11521 acaccaacat gtgttgctac accgccaaga acaattgtat caattcctcg acgtctcaac
    11581 tgtaagtcta aatccgttcc tacaaatgca ctaaattgtc gtttatctat gacaaaatcg
    11641 tcatctcttt tgtctagtaa ataatggaaa tggctgtagt catcgccttc ttttggtggt
    11701 aatgagatca ttgcatttgg ttgcaatgca tctttaccat catagaaatt cacgcgaaca
    11761 aaggcgataa agccattgtt ttttctaaaa gcttcaatta atttgttagc attttgaaca
    11821 acattttcag ctgtatgtgg cgcataatcc attttaagaa taccttgttg caggtcgatt
    11881 aacactaaag cggttttatc aaaattaatc atcttatatc agagtccttt tcatttaata
    11941 atatattcgc ttaaacttat accctgaata cgatgaaatt atttattttg ttgtgaaaaa
    12001 gctttagcaa tatcgatcag tttcttcggt gcatcttcga cagacatttt gacttcgaca
    12061 aaatgcatca catcgggatg gccattaatt gtattaaata cctcttgtaa atcttttgat
    12121 gattcaacgt catgaatttc aacattttta ccaccaaata cagctggtaa agctttataa
    12181 tcccacatgt gaatttcatt ataaggttca tacatgccgt gaataagtcg ttctaccgta
    12241 tagccgtcat tattaatcac aaataatacc ggtttaatat gctgtctaat catagttgaa
    12301 atagcttgaa cagttagttg caatgagcca tcgccaatta ataataagtt acgacgatct
    12361 ttgtctgcta attgtgaacc taatgttgca ggtaatgtat agccgataga accccataac
    12421 ggttgcccta taaaagtatt gcttttgtat aatgctaaat cataagcacc aaaaaatgat
    12481 gtaccttgat cagcaataat gacatcattt ggttttaaga aattttgcat cattttaaaa
    12541 taagtttgtt gtgttaatgg ttctgtgcca acagtataat cgggtgatgt tggacgatga
    12601 tatgcaggaa acgatgcgtt attcgtatat gatatatcgg tcaattgttg tagtaatgat
    12661 gatagagata tttcatcatt tgtaacatca tcaattttga tattgtgatg atttaacata
    12721 acgacatcat cgatattgaa ttggtatgaa aatcctgctg ttgctgaatc tgttaatttg
    12781 gctccaatat ttaaaattaa atcgctgttg tccacataat ctcgtatttt atcttcggca
    12841 attttcccat cgtaaatacc catataatat ggattttcct cattaaaagc accttttcct
    12901 aatgaaagtt gtgctactgg tatctgtgtt tgatttacaa aatcttctaa ttcttgatgg
    12961 aggtgaaaac tgttaatttc atgtccagta atgatgatag gctgcttcga ttgatgcaat
    13021 ttagatacta ataactctat atatgttgat gcatcagtat ctttggctgc cgttacttcg
    13081 aatggtgtcg gtatctcaat ttcagagatt gcgacatcga ttggtaaatg taaatgaact
    13141 gggcgtcttt cggcgattgc tgtattaatt aagcgtggta tttcagttgc tgcattttca
    13201 ggtgtgatat aaccttgtgc aacggttata tgtgcaaaca tttttcggta gtcgtcaaat
    13261 gtaccttcac caagtgagtg atgtacatat ttaccggctt gttcaacagc acgtgtcggc
    13321 gcacctgtaa tcgcaatgac aggtatgcgt tcagcatacg aacctgcgat accgttgacg
    13381 gcacttaatt cgccaacacc aaatgtagta actaatgcag cgagtccatt aagacgggca
    13441 taaccgtccg ctgcgtaact tgcgtttaat tcatttgtat ttcctaccca atctacattg
    13501 ggattactga taatatcgtc tagaaaagcg agattaaaat caccaggaac accaaaaatt
    13561 ttatcgacgc ctgctcgatg aatagcgtca attaagtaag ctccaatgcg ttgtttcata
    13621 aaaaaaccac cttttctcta tatttacact taaaatgata gcctcgaata tagtgtaagg
    13681 tcaaggaagt tgactttgtt gtgaaaacgc taacaatagt gagcaattaa tattttttag
    13741 gaaatgagta tgaaggggga tttaattgat gattttaaaa ttcaaaattt aaggggagag
    13801 cgaactatca atgatgctgc aaaacacaat cgcaatgaaa aaacaggcgc gtcaccttat
    13861 gaaggtatac gcacctgttt atagtaagca ttatttagct tcaaataatt gatcgccaaa
    13921 tgaaatgttg ccatgttcac cttgtttaaa atcaaggttt gtaatgtttc cttgtgtaac
    13981 gataataggc gtaatatcac tctttgcatg attgcggatg tagtctaaat caaagttgat
    14041 taataaatca ccttgtttaa cttcttgacc ttcctcaaca tgtaaagtaa agccttctcc
    14101 gtttaattta acagtgtcta aaccgatgtg gattaatagt tctaaaccac tatctgatac
    14161 aagaccaatt gcatgttttg ttgggaaaat catttgtact ttaccgttga atggtgcacg
    14221 aacttcacct tgtgaaggtt taatagcgat accgtcaccc atcatttttt cgctgaacac
    14281 ttgatcaggc acttctgata atggtgttac ttcaccagtt aatggtgcat gcacgatatg
    14341 gctcaattcg cttgttgcag atttatcttc tgcaacaaca acggtttcgt ctttatcgtc
    14401 ttccatagta gtagggtttt ctactacttg accattcata atctgttgca tttcatgttt
    14461 gatttggtca gattttggac caaaaattgc ttgcatatta ttgccgactt ctaatacacc
    14521 agatgcgcct aaatctttca aaccaggaac atcaacttta gatttgtcgt taacttcaac
    14581 acgtagacgt gtgatacaag cgtctaaatg tttaatgttt gctttgccac ccatagcttc
    14641 taatactgca tatggtaatt cagttgctga agcagtagcc gcttgtgatt gtttatcttc
    14701 acgacctggt gttttgtatt ttaattttac aattaggaat cggaatacga agtagtaaat
    14761 gactgcgtat acaagaccta caggaatgac taaccaccat tgtgtcttat taggtagtat
    14821 accgagtaag aagtagtcga tgaaaccacc tgagaatgta taacctagat gaagatctaa
    14881 taagtacaat gttaagaatg ataaaccatc aagtactgcg tgaataaaga ataataatgg
    14941 tgctacgaat aagaatgaga attctaaagg ttctgtaata ccagttaaga atgatgttaa
    15001 agcagcagaa cccattaaac cggctactac tttcttgttt tcaggtttag ctgtgtgata
    15061 aattgctaaa gctgctgcag gtaaaccgaa catcataaca gggaattcac cttgcatgaa
    15121 tttaccagct gttaaatgtg caccttcacg aatttgttcg ataaagatac gttggtcacc
    15181 gtgaataatt tcacctgctg catttttcca tgaaccaaac tcgaaccaga acggtgcgtg
    15241 gaaaatgtga tgtagaccga atggaattaa taaacgcttg atgaaaccaa ataagaatac
    15301 ggcaacacca gtatttgagt ctaataatcc tgtactgaat gcatttaatc ctgtttggat
    15361 tgttggccag attaatgcca ttgggaatgc taaaataaat gatgttgtag ccatcataat
    15421 aggtacgaaa cgcttaccag cgaagaaacc taaataagat ggtaagttaa tgttatagaa
    15481 cttgttataa caccaagctg ccagggcccc gattataata ccgccgaaca cacctgtttg
    15541 taatgttggg atacctaaaa tgctagcgta accactcgct ggatcaccaa tgttcttagg
    15601 tgttacttgt aaaaagtcac ccattgtttt gttcatgatt atgtaaccga cgaatgctgc
    15661 gatagctgct acaccatcac cgccagctaa tccgattgcg acacctaatg cgaaaatcat
    15721 aggcaagtta tcaaaaatga taccaccagc acctgtcatt aatttagcga cagtttgtac
    15781 gccaccattt tgtataaaag gcaagtagtg ttgtaatgct tcgccttgca tagctgtacc
    15841 gatagctaat aacagacccg ctgctggtaa aatcgcaaca ggtaacatta gtgccttacc
    15901 aatacgttgc aattgaccga aaagtttctt cctcacttgt ccaacctcca aagttgtatt
    15961 tattatttaa gattcaataa aaaagacacg agcaaataga ctgtgttggt aaccatcaca
    16021 gcttaattta actcatgcct aatcttactt agtaacacgt tggtgtatgt aattaatgta
    16081 aaagaagcaa ttgactaagt agtatataaa acggatacgt tagtttatct agcttaccat
    16141 cacatcttat tgaatcttat ttgtttgcga ctcctatttt agcatggtga agtatgcgtt
    16201 ttcaatacaa ttattaaatt caatttcaaa tctcttaatt gttcacagat tattattaaa
    16261 acataatata agataatcct gaataaaatg tataaaagtt gttgtaacaa ttcagcaata
    16321 gcaactgttt tcgagcgtta aatgataaga aaaatatgtc gtttaaaaaa attaacttgt
    16381 gacagaaata tgtaagatta ttacaatgaa caggctttat ctgattaata ttgtaatgtg
    16441 ttttctctta tagtaaaatg taagcgatta cacaactcaa ctatattttc ctaataatta
    16501 tattgttaag gagggctact ttgacaggct tttcagtgta tttaggacaa cctttagatg
    16561 aagcgtatat taagcgaatg attaaacaag gttaccaaat gatttttaca tctgtacaaa
    16621 taccagaaga agatgacgag acaaaatatc attatttcac aaaactactc aatttattaa
    16681 aacatgaaca agtgacttac ctcatagatg ctaatccatc tatattaaca ccatcttttt
    16741 atgagcatct tcgacaatat gatgcacaat ttatgattcg tatcgatcat agtacatcaa
    16801 ttgaggcaat cgaagcgata atggcacagg gtttaaagtg ctgtttgaat gcaagtatta
    16861 tttcccggga attgttaaca agcttacatc aacaattgaa tgattttaca ttactttcat
    16921 tttgtcataa ctattatcca agaccagata cgggattatc tgttgacttg gtcaataaga
    16981 aaaatgaact catttatcaa tttaatccaa aggcacaaat atatggtttt attgtaggga
    17041 gtggtttgcg aggtcctttg cataaaggct tgccaacaat tgaagcaacg agacatagtc
    17101 atcctgtcgt tgcagctaaa ttattacaag aaactggtgt atctgaagtg ttagttggag
    17161 actcattgat tgaaataagg caggcaaaac aacttataga tttttgtaag cataggcatt
    17221 tcacgttatg tattgaagaa gtgtttgata cgacagtgac ttaccttttc gatatgtgtc
    17281 ataaagtacg tccggataat ccggagaatg tcattcgttc ggaaacgtca agacaaatat
    17341 gtccgcattc gattcaacca cagtttacga cgcaacgacg cattggttca gtaaccgttg
    17401 ataatttgaa taacggacgt tatcaaggtg aaatgcaaat tgtgagacaa acgcttagtg
    17461 cacatgacag tgtgaatgtt gttgcacaaa ttattaaaga agacttacca ctgttaagtt
    17521 gtatcgagcc gaatgataca tttgattttc aaaaaactag ggagtgtaag aagtgatgga
    17581 aaatagtacg accgaagcgc gtaatgaagc gacgatgcat cttgatgaaa tgactgtgga
    17641 agaggcatta attacgatga ataaagaaga tcagcaagtc ccgttagcag ttcgaaaggc
    17701 aataccacaa ttgacaaaag taattaaaaa aacaattgca cagtataaaa agggtggacg
    17761 attgatctat atcggtgcag gtacaagtgg acgattgggt gtcttagatg cagcggagtg
    17821 tgtacctaca ttcaatactg atcctcatga aattataggt attattgctg gtggacaaca
    17881 tgctatgacg atggctgtag aaggtgcgga agatcacaaa aaattagcgg aagaagattt
    17941 gaaaaatata gatttaacat caaaagatgt cgttatagga attgccgcga gtggcaaaac
    18001 gccatatgtt ataggcggtt taacatttgc taatacaatc ggtgctacaa cagtatctat
    18061 ttcatgcaat gaacatgcag ttataagtga aattgcgcag tatccagtag aagttaaagt
    18121 tggtccagaa gtattaactg gttcaacgcg tttaaagtct ggtacagcac aaaaattaat
    18181 tttaaatatg atttcaacca tcacaatggt tggtgtcgga aaagtttacg ataacctcat
    18241 gattgatgtt aaagcaacca atcaaaaact gatcgaccgt tcagtgcgta ttattcaaga
    18301 aatatgtgct atcacatatg atgaagcaat ggcgttatat caggtatctg agcatgatgt
    18361 gaaagttgcg acagttatgg gtatgtgtgg catttctaag gaagaagcaa caagacggtt
    18421 attaaacaat ggtgacattg ttaaacgagc aatcagagat agacaacctt aggagggatt
    18481 taaatgacca aagaacaaca acttgcagaa cgaattattg ctgcagtagg tggtatggat
    18541 aatatagata gtgtcatgaa ctgtatgaca cgtgtgcgta ttaaagtatt agatgaaaat
    18601 aaagtagatg accaagaact aaggcacatt gatggtgtca tgggtgttat acacgatgaa
    18661 cgcattcaag ttgtagttgg acctggtaca gtcaataaag tggctaatca tatggctgag
    18721 ttaagtggtg ttaaactagg tgacccaata ccacacaatc acaatgatag tgaaaaaatg
    18781 gactataaat catatgcagc tgataaagca aaggcaaata aagaagcaca taaagcaaaa
    18841 caaaagaatg gtaagttgaa taaagtattg aaatcaattg ccaatatctt tataccgttg
    18901 attcctgcat ttattggagc tggattaatt ggtggtattg cagcagtact gagtaactta
    18961 atggtggcag gctatatttc aggtgcttgg attacgcaac ttataacagt atttaatgtc
    19021 attaaagacg gtatgttagc atacttagct attttcactg gtattaatgc ggctaaagaa
    19081 tttggtgcga caccaggact tggtggcgtg attggtggta caacgttatt aacgggtatt
    19141 gctggtaaaa atattttaat gaatgttttc actggagaac cattgcaacc tggacaaggt
    19201 ggaattattg gtgttatttt tgccgtttgg attttaagta ttgtcgaaaa gagattgcat
    19261 aaaattgtgc caaatgcgat tgatattatt gtaacgccga ctattgcatt gttgattgta
    19321 ggactattaa ctatctttat ctttatgcca ttagcaggtt ttgtttcaga cagtttagtt
    19381 tcagtagtta acggaattat tagtattggt ggcgtattta gtggatttat cattggtgca
    19441 agcttcctac cgttagttat gttagggctt catcatattt ttacgccaat tcatatagaa
    19501 atgattaacc aatctggtgc tacttactta ttgccaattg cagcgatggc tggtgctgga
    19561 caagtaggtg ccgcattagc actttgggta agatgtaaac gcaacacaac attacgtaat
    19621 actttaaaag gtgcattgcc agttggtttc ctaggtattg gagaaccatt aatctatggt
    19681 gtgactttgc cattaggtcg acctttctta actgcttgta ttggcggtgg tattggtggc
    19741 gctgtaatag gtggaattgg acatattggt gccaaagcaa taggcccaag tggtgtgtca
    19801 ctattaccat taatctcaga taatatgtat ttaggttata ttgcaggatt acttgctgcg
    19861 tatgctggtg gattcgtttg tacatattta tttggaacga caaaggcgat gcgacagaca
    19921 gatttgttgg gtgattaatg atgacaaata ttttatatcg cattgataag cagttgagtg
    19981 attttacgaa gacagaaaag ataatcgctg attacatttt aaagaatcca cataaaatca
    20041 ttgatatgac tgtgaatgat ttagcagatg ttacgaatgt tagtacagca tcaattgtta
    20101 gatttagtcg gaaaatgaca catcaaggtt ttcaagagct aaagattgcg atatctcgat
    20161 acttacccga agatattgca accaatccac atttagaatt gattgaaaat gaatctgtag
    20221 aaactttgaa aaataaaatg attgctagag caacgaatac gatgcgattt gtagctacta
    20281 atattatgga tgcgcaaatt gatgcaattt gtgatgtgtt gaaaaatgcc aggacaatat
    20341 ttttatttgg atttggcgca tcgagtttga ctattggtga tctttttcaa aagttatctc
    20401 gtattggctt aaatgtcagg ttattacatg aaacgcattt acttgtgtca acatttgcga
    20461 cgcatgatga tagagattgc atgatttttg tgacgaatca aggtagtcat agtgaattgc
    20521 agtcaattgc acaggtggcc acacattaca gtattcccat cataactata tctagtacag
    20581 ctaataatcc agtggctcaa attgcagact atgcattgat ttatggcaga actgatgaaa
    20641 atgaaatgcg tatggcggct acaacgtcac tatttgcaca gttattcacg gtagatatat
    20701 tgtactatcg atttgtagca ttaaattatc atgcgattct agattgtata acccaatcga
    20761 aaatggcact tgataattac aggaagcatc ttgcgacgat agattttaaa cattagatat
    20821 tgtgtgcgtt ctaatataaa gttagttgca acgtaaacaa ttgaagtttt tgatgtgatt
    20881 tagttgcttg atgaaaaatt gttgatacaa gtttgtaaaa ctggcataga agttattttg
    20941 aaaatgataa aagtgtgaca aattaaagca aagttcaggt ttgagctttg cttttttcat
    21001 agtatagaaa ttaaatgttg aagaataatt ttaatgctaa atgaactaat acgaaggtgc
    21061 taaaagtaat tattatcaaa ttagtgatat gaatgaaatg tttaaatcgt ttgcgttgat
    21121 tgtattcggg acttttatag agtaggtagt agtacataaa gagtatggct gcgaacatgc
    21181 cgaatattga agtgaagtta cctacagtga ctgccatgaa atgatttaaa gttaaataat
    21241 aacttagact gataattaaa aataaaatga tttgataggt aattaacacc attcaagtcc
    21301 ctccattaat cgtaggcaaa aaatttatca atgatttgat tatttgtatt caaattttag
    21361 tatacgactt acctcaaaca caaatattaa aatataagac aataaaatta gaataaattt
    21421 acgtttgaag ataaaaaaca aacatttttc attaaagttt atgatatatt tagagcaata
    21481 gaaagtgtat ggaaggggat atgaatggca taccaaagtg aatacgcatt agaaaatgaa
    21541 atgatgaatc aacttgaaca attgggttac gaaagagtaa cgatacgtga taataagcaa
    21601 ttgcttgata attttagaac gattttaaat gagcgtcatg cggacaaatt agaaggcaat
    21661 cccttaacag ataaagaatt tcaacgtctg ttaacgatga ttgatgggaa aagtattttc
    21721 gaaagtgccc gtattttacg tgataaatta ccacttagac gtgatgatga gtctgagatt
    21781 tatttgtcgt ttttagatac gaaaagttgg tgtaaaaata agtttcaagt gacgaatcaa
    21841 gtatcggtcg aggatacata taaagcacgt tatgatgtaa cgatattaat caacggacta
    21901 ccccttgtcc aagttgaatt gaaacgtcga ggcattgata ttaatgaggc gtttaaccaa
    21961 gtaaaacgtt accgtaaaca aaattacaca ggcttattcc gctacataca aatgtttatc
    22021 attagtaatg gtgttgaaac gcgatacttt tctaataatg atagcgaact attgaagagt
    22081 cacatgtttt attggagtga taaacagaat aaccgaatca atacattgca atcgtttgct
    22141 gagtcattta tgagaccttg tcaattagct aagatgatat cacgctatat gattattaat
    22201 gaaacagata gaatactgat ggcaatgcgt ccgtatcaag tgtatgcggt agaagcactt
    22261 attcaacaag cgactgagac agggaataat ggatatgtgt ggcatacaac gggaagtggt
    22321 aagacgttga cttcttttaa agcgagtcag attttatcac aacaagatga cattaagaaa
    22381 gttatctttt tggttgaccg taaagacttg gatagtcaaa cagaagagga atttaataaa
    22441 tttgctaagg gtgctgtcga caaaacattt aatacctcgc aactggtacg ccaactaaat
    22501 gataaaagtt tgccacttat tgtaacgacg attcaaaaaa tggctaaagc gattcaaggt
    22561 aatgcccatt tattagaaca gtataaaaca aataaagttg tatttattat tgatgagtgt
    22621 catcgcagtc aatttggtga catgcatcgt ctagttaaac aacatttcaa aaatgcccaa
    22681 tattttgggt tcactggtac gccacgtttc ccagaaaata gtagtcaaga tggtagaaca
    22741 actgcagata tttttggtag atgtttacat acgtatttaa ttagagatgc cattcatgat
    22801 ggtaatgtac ttggtttctc agttgactat attaatactt ttaaaaataa agctttaaaa
    22861 gcagaagata acagcatggt tgaagcaatt gatacggaag aagtatggtt agcggataaa
    22921 cgtgtggaat tagtaacacg acatatcatc aataatcatg ataaatatac acgtaatcgt
    22981 caatattcaa gtatatttac agttcaaagt attcacgcgc ttattaaata ttatgagaca
    23041 tttaagcgac ttaacaaaaa gttggaacaa ccattaacgg tagctggtat atttacgttt
    23101 aaacctaatg aagatgatcg tgatggtgaa gtgccatatc attcacgtga aaaattagag
    23161 ataatgatta gtgattataa taaaaagttc gagacgaatt tttcaacaga cacaactaat
    23221 gagtatttta accatatttc aaaaaacgtt aaaaagggcg ttaaagatag taaaattgat
    23281 atcttaatcg ttgttaatat gttcttaact ggttttgata gtaaagtact gaacacttta
    23341 tatgttgata agaatttaat gtatcatgat ttaattcaag cgtattcacg tacaaatagg
    23401 gttgaaaaag aatcaaagcc atttggtaaa attgtaaact atcgtgactt gaaaaaagag
    23461 acagacgatg cactgagagt attctcacaa acaaatgata cggatacaat tttaatgcgt
    23521 agttatgaag agtataaaaa agaatttatt gacgcttatc gtgagcttaa aatgattgtg
    23581 ccgacaccac acatggttga tgacattcaa gatgaagaag agctaaagcg ctttgttgaa
    23641 gcttatcgtt tattagctaa aataatatta cgtttaaaag catttgacga gtttgagttt
    23701 acaattgatg aaattggaat ggatgaacaa gagaatgaag actataaaag taaatattta
    23761 gccgtgtacg atcaagtaaa aagagcgacg gctgagaaaa ataaagtatc cattttaaat
    23821 gatattgatt tcgaaataga aatgatgcgt aatgatacga ttaatgtgaa ttatattatg
    23881 aatatattga gacaaattga tcttgaagac aaagcggaac aacgtcgtaa ccaagaacaa
    23941 attagacgta ttttagatca tgcagatgat ccgacattga ggttaaaacg agatctaatt
    24001 agagaattca tcgacaatgt tgtaccttct ttaaataagg atgatgatat cgatcaagaa
    24061 tatgttaatt tcgaaagtat taaaaaagaa gcggagttca aaggatttgc tggagagaga
    24121 tctatcgatg aacaagccct aaaaacaatt tcaaatgact accagtatag tggtgttgta
    24181 aacccacatc accttaaaaa aatgattggt gatttgccat tgaaagaaaa acgtaaagca
    24241 agaaaagcca ttgaatcttt cgtggcagaa acaactgaaa aatacggtgt gtaatgattc
    24301 agccccctcg ctagattagt gtagggggca ttattatttc attagaaaag gctcatgtgt
    24361 ttggcaatca tcatttggtt tcaatacaat tttatcatcg tatttgtata gaaataatgt
    24421 ctataggcat ttaaaatagt ttataatatt aacaggataa gtattaaatg tatgttgaag
    24481 gaggtgcgta gctatggata atagaaatat gattaatcgt gtttttagtc aaaagatatt
    24541 acatcaaatt gcaatcaaaa ataaaagtga tgttgttgat gaggcatatg atttttatat
    24601 acaagggcct aaaaatatca atgtaataca gaaaatgaag tctttatata actatcttaa
    24661 aaagtcttat cgtaacgaat atttttataa aaacacaatg cttaataaac tccttttagg
    24721 actacattct gttaatacaa ctactgcact ttctgagatg cctataggaa atagtattgc
    24781 tgattttata ttgttaaatg gtaaaggcgt tgtctacgag attaaaacag aattagataa
    24841 gttggataga ttggataatc aaattaatga ttattatgaa gtatttaatt atgtagtagt
    24901 cattacaaat gacaaacact tgaataaagt tatggctaga tacaaagata caacagtcgg
    24961 aattttagta ttaacatcta gaaatacact gagtgaagtt caaaaaccaa aagaaaacaa
    25021 tagtctctta aactcaaaag cgatgtacaa ctttttacga aaagaagaaa gaaaaagagt
    25081 tattgcacaa aatcatatgg atgtgccaac ttataatgat ttcacagagt atgatgtgtt
    25141 atttgacgtg tttaaagaaa taccaatgac gaaactgcat aacaatatga tttttgagtt
    25201 gaaaaaaaga ggcaacatga aagaatacaa agatgaattt ttagcagctc cgactgaaat
    25261 taagttcttg ttatatttcg caaaaatgac aaagaaagat aaaaataaac tatatcattt
    25321 tcttaaggat acaaataatc ccccgcactt cacatagtgg cgggggattt atcatgcgtt
    25381 aaaatgccca aagtatgggt gtgaccctta aatcatggca aagcatctaa cacttgatat
    25441 ggtgccgagc cctaacatag cacaatatag ttagtatgac aaagtcatct aacataacac
    25501 atcagatgtt gcccgattta ttccagacca tacagtgata aagttgcaca acgcatgact
    25561 tttatttagc aataactgct acttctgaaa taagttgctt tgcatagtct gattgcggat
    25621 gtttgataat atcttctgtg ttattaagtt caacgatttc gccatttttc ataactgcaa
    25681 ctcgatcaca tatttcattg ataacaccca tgtcatgtgt gatgaataaa taagtgatgc
    25741 cgaagtctaa ctgtaattgt tttaataact cgatgatatc tttttgaatt gaaacgtcta
    25801 aagctgatac tgcctcgtcg caaacaatca ctttaggttc tacagcaagt gctcttgcga
    25861 tacttacacg ctggcgttgc ccaccagata attcgtgtgg atagcgatat aagaaacttt
    25921 gatctaggcc aactttttct aacaacgata cgatagtttt aataatgtcg tcattatctt
    25981 tgactttccc atgaatgatt agtggtcgtt taatcacatc aatgacttta aatcttggat
    26041 taatagatgc gaatggatct tggaaaatca tttgtatctc ttgtcgtaaa gatttcaatt
    26101 catcatcttt aaataaactt aatggtaatt cgttatacca aataaagcct tctgacactt
    26161 cctttagacc gacgaccgtc ttagctaatg tcgatttccc tgaccctgat tcaccgacaa
    26221 tgcctaacgt ttcgcctttt ctaatagcca agttaatatc attaactgct cggtataggc
    26281 tgccactcgg tgatgtgtaa tccacgctca cgcgatcgaa ttttaataaa atatcattgt
    26341 ttaacggtct tggcggacgc gtttgatgaa tatcaggaat cgcatctatt aagcgtttcg
    26401 tataggtatg ttgtggcgat ttaaaaatac tttcaaccgt gccactttca acgacacttc
    26461 catctttcat tacaatcaca tcgtcgcaaa attgatacac agcacctaaa tcgtgagtga
    26521 taaaaataat agacgtttct gtgtactcat aaagggactt cattaactgc agtaattgat
    26581 tttgtgtact ggcatctaat gccgttgttg gttcatctgc gattaaaatt tgtggcttta
    26641 aaatcaatgc cattgctatc atgacacgtt gacgcatacc accagaaagt tcatgcggat
    26701 aagcatcaaa ttgtcgagtt gcatgtttta tacctacttt ttctaaaatg tctattgtca
    26761 tcgactttgc ttcagattta gatacacgtt tatgttgaaa tagtacttct gtaatttgtt
    26821 tgccaatcgt taatcttgga ttcaacgaag agagtggatc ttgaaaaatc attgaaatat
    26881 ccttgcctcg aatttgttgt aacgctgaag ttgataaatt agttaacgat tgcccattaa
    26941 aaataatttc tcctgttaac gtgtgatccg gataatctgg tagtagccct aaaatagatt
    27001 tagcggtaat actttttcct gatcctgatt caccaacaat acctaggata tgtttttttc
    27061 gtaattcgaa agagacgttt tttaccgctt gaactgtagt ttcatcataa ttgaattgta
    27121 cattcagact gttgacttct aataaatttg acatgatgtt gtgcctccct attcacaaat
    27181 gtgtaaattt attctatact tttgtatatc atagtaatct actcaagatt attttacaag
    27241 gagtgattag cataattact tatttagaat cttttaagtc gttgtatcac aaggcatttt
    27301 acaagtttgt attatcggta ttaagcttgc cattattttt atatgcagtg ataaagtttt
    27361 tcttttctgc taagagaaaa aatttttacg ctaataattc tgaaatttca gaaatcgaac
    27421 aggcgttaca tcaaaaatat aaatatttat cgcagcaaaa gtcatccaca caaatacata
    27481 aagaagcatt aaaaatattc aagacacaaa gttctaatac gaattcaaag aatattgaac
    27541 aagcacattt ttcaacatac tttgaaaatg tattatttca taagttcatc attatcaaag
    27601 tgatattggc gttaccgatg ttcatcttat tgactattta tttacagcca ttagttagat
    27661 atatttttga gcgaattgtc atggctgtga ttgtcatcat tggtgttatt gtcagtgtgt
    27721 ttagcattct gtatttttca ccgcttgatg cggcttatag catactggga caaaatgcaa
    27781 caaaggcaca gatacatcaa ttcaatgtat tacatcatct taacgaacct tattttattc
    27841 aattgtggga taccatcaag ggtgttttta cctttgactt aggtacgact tacaaaggga
    27901 atgatgttgt gactaaagca gttggcgaaa gaattccaat tacaataatt gtcgcagtat
    27961 tagcgctaat tgtggcatta attattgcaa taccaattgg tattatcagt gcgatgaagc
    28021 gaaatagttg gcttgatatc acgttaatga taattgcatt aattggtttg tctattccaa
    28081 gtttctggca agggttatta ttcattttag cgttctcatt gaaattggat attttgccac
    28141 catcttatat gccagaacat ccaatatcgt tgattttacc tgtacttgtc attggaacaa
    28201 gtattgctgc ttctatcacg cgtatgacaa ggtcttctgt acttgaagta atgcgcagcg
    28261 attatgtttt aactgcttat gcaaaaggat tatcgacgac acaagtggtt attaaacata
    28321 ttttgaaaaa tgccattatt ccaattgtaa cgctagttgg tcttctagtg gcagagttac
    28381 taggcggttc agcagtgacg gagcaagtat ttaacattaa tggtatcggt cgttatatcg
    28441 tccaaaaaca actaatacct gatatcccag cagtcatggg tggggtcgta tatatatcaa
    28501 ttgtaatatc tttagcaaac ttaattattg atatatttta tgctttaatc gatccaaaat
    28561 tacgtagtga aattaacgaa aggaagtgag gcatatggta aaacttacaa caaagatagc
    28621 ttccttaaaa ctattcgcaa gttatgctat tgcaacttat attttagtta tattaacgag
    28681 tgcattaaat ctttttaaag gttatgtggc cgatacgttc tatattgctg aaacattgct
    28741 aatcgtttta accatcattt taattatcat cttaacaacg gaacaaacat ggaagcatca
    28801 tgacctatgg cgacgtatcg tcgaagtgtt gttattgttg atgacattaa cagggaacgt
    28861 atttacatta ttaatgtttg taagtattag acgttaccaa cgtacatcgc aaatacatag
    28921 ttataatggc tgggaatcgt ttatacgaaa aactactaga catcgtattg cgattatcgg
    28981 gttacttatt ttagtctata tgctgacatt atcaattgtg tcacaattta catttgatac
    29041 gacattggct actaaaaatc agttcaacgc actgttacat ggaccgagtc tagcctatcc
    29101 gtttggtact gatgatttcg gtagagactt atttacacgc gtagttgtcg gaacgaagct
    29161 gacattttca atttcaatta tttcagtagt tattgcagtt gtttttggtg tgttactagg
    29221 cactatcgca ggttatttta atcatattga taatttaata atgcgaattt tagatgtagt
    29281 gtttgcaatt ccatcattat tgttagcggt ggcaattatt gcatcgtttg gagcaagtat
    29341 tccaaattta attattgctt taagtatcgg taatatacca tcatttgcac ggacaatgcg
    29401 tgccagtgtt ttagaaatta aacgcatgga atatgtagat gcagcacgta tcactggtga
    29461 aaacacttgg aa
//
LOCUS       DAMZPP010000013        29034 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMZPP010000013 DAMZPP010000000
VERSION     DAMZPP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 29034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29034
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.013"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..497
                     /gene="fmhC"
                     /locus_tag="POH73_000450"
     CDS             <1..497
                     /gene="fmhC"
                     /locus_tag="POH73_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HDI6886428.1"
                     /translation="LKIQLLKIENEMMTVNRALNENPNSKKNKSKLNQLNMQLSSINN
                     RISKTEELILEDGPVLDLAAALFICTDDEVYYLSSGSNPKYNQYMGAYHLQWHMIKYA
                     KSHNINRYNFYGITGVFSDEADDFGVQQFKKGFNAHVEELIGDFIKPVRPILYKFAKL
                     IYKV"
     gene            669..1541
                     /gene="dprA"
                     /locus_tag="POH73_000451"
     CDS             669..1541
                     /gene="dprA"
                     /locus_tag="POH73_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDI6886429.1"
                     /translation="MIRLFLLKLYWAHFSTKQIHQFLMAYPNVIKEGGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDTEYPVLLKEIYQFPLLLF
                     YRGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEIGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
     gene            1715..3790
                     /gene="topA"
                     /locus_tag="POH73_000452"
     CDS             1715..3790
                     /gene="topA"
                     /locus_tag="POH73_000452"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDI6886430.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            3946..5253
                     /gene="trmFO"
                     /locus_tag="POH73_000453"
     CDS             3946..5253
                     /gene="trmFO"
                     /locus_tag="POH73_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HDI6886431.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLSERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEAAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
     gene            5671..6567
                     /gene="xerC"
                     /locus_tag="POH73_000454"
     CDS             5671..6567
                     /gene="xerC"
                     /locus_tag="POH73_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDI6886432.1"
                     /translation="MNHIQEAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLQL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKSLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            6564..7109
                     /gene="hslV"
                     /locus_tag="POH73_000455"
     CDS             6564..7109
                     /gene="hslV"
                     /locus_tag="POH73_000455"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDI6886433.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            7175..8578
                     /gene="hslU"
                     /locus_tag="POH73_000456"
     CDS             7175..8578
                     /gene="hslU"
                     /locus_tag="POH73_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDI6886434.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLAVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            8603..9376
                     /gene="codY"
                     /locus_tag="POH73_000457"
     CDS             8603..9376
                     /gene="codY"
                     /locus_tag="POH73_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDI6886435.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            9427..9519
                     /locus_tag="POH73_000458"
     CDS             9427..9519
                     /locus_tag="POH73_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886436.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDIPH"
     gene            9718..10494
                     /gene="rpsB"
                     /locus_tag="POH73_000459"
     CDS             9718..10494
                     /gene="rpsB"
                     /locus_tag="POH73_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002448842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDI6886437.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEATEE"
     gene            10528..10641
                     /locus_tag="POH73_000460"
     CDS             10528..10641
                     /locus_tag="POH73_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886438.1"
                     /translation="MGDKILMLITFFKKKIKANYKYSIRVLAILPIIMLKL"
     gene            10676..11557
                     /locus_tag="POH73_000461"
     CDS             10676..11557
                     /locus_tag="POH73_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDI6886439.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            11694..12416
                     /locus_tag="POH73_000462"
     CDS             11694..12416
                     /locus_tag="POH73_000462"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDI6886440.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            12435..12989
                     /gene="frr"
                     /locus_tag="POH73_000463"
     CDS             12435..12989
                     /gene="frr"
                     /locus_tag="POH73_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDI6886441.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            13362..14132
                     /locus_tag="POH73_000464"
     CDS             13362..14132
                     /locus_tag="POH73_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDI6886442.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            14139..14921
                     /locus_tag="POH73_000465"
     CDS             14139..14921
                     /locus_tag="POH73_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDI6886443.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
     gene            15133..16419
                     /gene="rseP"
                     /locus_tag="POH73_000466"
     CDS             15133..16419
                     /gene="rseP"
                     /locus_tag="POH73_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDI6886444.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGI
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTERKLTK
                     VSSETKYVLGFQPASEHTLFKPIVYGFKSFLIGSTYIFSAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            16439..18142
                     /locus_tag="POH73_000467"
     CDS             16439..18142
                     /locus_tag="POH73_000467"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDI6886445.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKAGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPIEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            18406..22716
                     /locus_tag="POH73_000468"
     CDS             18406..22716
                     /locus_tag="POH73_000468"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDI6886446.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKSKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEYKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGETLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            23006..23473
                     /gene="rimP"
                     /locus_tag="POH73_000469"
     CDS             23006..23473
                     /gene="rimP"
                     /locus_tag="POH73_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDI6886447.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            23494..24669
                     /gene="nusA"
                     /locus_tag="POH73_000470"
     CDS             23494..24669
                     /gene="nusA"
                     /locus_tag="POH73_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDI6886448.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVTDVSVETNVEKESE"
     gene            24690..24974
                     /locus_tag="POH73_000471"
     CDS             24690..24974
                     /locus_tag="POH73_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDI6886449.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            24971..25288
                     /locus_tag="POH73_000472"
     CDS             24971..25288
                     /locus_tag="POH73_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HDI6886450.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SNNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            25293..27410
                     /gene="infB"
                     /locus_tag="POH73_000473"
     CDS             25293..27410
                     /gene="infB"
                     /locus_tag="POH73_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDI6886451.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQSQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIVQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            27797..28147
                     /gene="rbfA"
                     /locus_tag="POH73_000474"
     CDS             27797..28147
                     /gene="rbfA"
                     /locus_tag="POH73_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDI6886452.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            28317..>29034
                     /gene="truB"
                     /locus_tag="POH73_000475"
     CDS             28317..>29034
                     /gene="truB"
                     /locus_tag="POH73_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDI6886453.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLEMKGVHSANFNNDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLK"
BASE COUNT        10768 a         3972 c         5659 g         8635 t
ORIGIN      
        1 aattaaaaat acaattattg aaaatcgaaa atgaaatgat gactgtgaac agagcattaa
       61 atgaaaatcc aaattctaaa aagaataaat ctaaattaaa tcagttaaat atgcaattat
      121 ctagtattaa taatagaatt agtaaaaccg aagaactaat attagaagat ggacctgttt
      181 tggatttagc tgctgcttta tttatatgta ctgatgatga agtttattat ctatcaagtg
      241 gatcaaatcc taaatataat cagtatatgg gcgcatatca tctacaatgg catatgataa
      301 aatatgcaaa atcacataat attaataggt ataattttta tggaataaca ggcgtcttta
      361 gtgatgaggc ggatgatttt ggtgtacaac aatttaaaaa aggttttaat gcacatgttg
      421 aagaattaat tggtgatttc atcaaaccag taagaccaat tctatataaa tttgcaaaac
      481 ttatttataa ggtttgatta taaagtatgt tggaaattga aatttaaaat tctttccaac
      541 atacttttca ctttttaagt cggtatttta ctccttaaaa taacattaca tcgacaatgt
      601 ttttgtatta gaaatgtaaa acaaaaatag aatatgattt tgtatgatta aaaaaaggag
      661 ttgatttatt gattagacta tttttgctta agttatactg ggcacacttt tcgactaaac
      721 aaattcatca atttttaatg gcatacccta atgtaattaa agaggggggc agaaaaaaag
      781 atagttattt atgtgaatgg gtgaataggg aagaaaatgt tcatttatta cgtaaatact
      841 atgcttttat aaaacttgat cataacgata ttattaaaga actgcagaaa ttaaaagtaa
      901 gttacattac atatatggat actgaatacc cagtgctatt aaaagaaata tatcaatttc
      961 cattacttct tttctataga gggaacatca aattaataaa taatatgcat catttggcag
     1021 tagtaggtgc aagagattct acaagttata cccaacagtc tttagaattt ttattatcaa
     1081 atgataaaag caaatattta acaattgttt ccggccttgc tcaaggagct gatgcaatgg
     1141 cacatcaaat agctttaaaa tacaatctcc ctacaatagc agttttagcc tttggccatc
     1201 aaacacatta tcccaaaagc acattagcat taagaaataa aatagaagaa ataggtttag
     1261 ttatatccga atatccacca catacaccaa ttgctaaata tagatttcct gagcgcaata
     1321 gaattatcag cggtttgtca aaaggggttt taattactga ggctaaggaa caaagtggta
     1381 gtcacatcac gatagatttt gcattagagc aaaatagaaa tgtttatgtt ttacctggat
     1441 ctatgtttaa tcctatgaca aaaggtaatt tattacgtat ccaagaaggt gctaaggtag
     1501 tattaaacgc taatgatata tttgaagact actatattta aaactaaaaa taacgagttg
     1561 cttcatattt cttttgttac cctttgtaat ttatttttta ataaaatcta taaaaaatag
     1621 acagggaaaa tgatttgttt agatataaaa cgttgacaaa agcaaaatta agcgtttatc
     1681 atttatcttt agtaattaga ttagcgaggg ggaaatgaca ttggcagata atttagtcat
     1741 tgttgaatcg cctgcaaaag caaaaaccat tgaaaagtat ttaggtaaga aatataaagt
     1801 tatagcttca atgggacacg tcagagactt accaagaagt caaatgggtg tcgacactga
     1861 agataattac gaaccaaaat atataacaat acgcggaaaa ggtcctgttg taaaagaatt
     1921 gaaaaaacat gcaaaaaaag cgaaaaacgt ctttctcgca agtgaccccg accgtgaagg
     1981 tgaagcaatt gcttggcatt tatcaaaaat tttagagctt gaagattcta aagaaaatcg
     2041 cgttgttttc aacgaaataa ctaaagacgc tgttaaagaa agttttaaaa atcctagaga
     2101 aattgaaatg aacttagtcg atgcacaaca agcgcgtcga atattagata gattagttgg
     2161 ctataacatc tcgccagttc tatggaaaaa agtaaaaaaa gggttgtcag cgggtcgagt
     2221 tcaatctgtt gcacttcgtt tagtcattga ccgtgaaaat gaaatacgaa actttaaacc
     2281 agaagaatat tggactattg aaggagaatt tagatacaaa aaatcaaaat tcaatgctaa
     2341 attccttcat tataaaaata aaccttttaa attaaaaacg aaaaaagatg ttgagaaaat
     2401 tacagctgca ttagatggag atcaattcga aattacaaac gtgactaaaa aagaaaaaac
     2461 gcgtaatcca gcaaacccat ttacaacttc tacattacaa caagaggcgg cacgtaaatt
     2521 aaactttaaa gctagaaaaa caatgatggt cgcacaacaa ttatatgaag gtatagattt
     2581 gaaaaaacaa ggtacgattg gtttaataac atatatgaga accgattcta cacgtatttc
     2641 agatactgcc aaagctgaag caaaacagta tataactgat aaatacggtg aatcttacac
     2701 ttctaaacgt aaagcatcag ggaaacaagg tgaccaagat gcccatgagg ctattagacc
     2761 ttcaagtact atgcgtacgc cagatgatat gaagtcattt ttaacgaaag accaatatcg
     2821 attatacaaa ttaatttggg aacgatttgt tgctagtcaa atggctccag caatacttga
     2881 tacagtctca ttagacataa cacaaggtga cattaaattt agagcgaatg gtcaaacaat
     2941 caagtttaaa ggatttatga cactttatgt agaaactaaa gatgatagtg atagcgaaaa
     3001 ggaaaataaa ctgcctaaat tagagcaagg tgataaagtc acagcaactc aaattgaacc
     3061 agctcaacac tatacacaac cacctccaag atatactgag gcgagattag taaaaacact
     3121 agaagaattg aaaattgggc gaccatcaac ttatgccccg acaatagata cgattcaaaa
     3181 gcgtaactat gtcaaattag aaagtaagcg ttttgttcct actgagttgg gagaaatagt
     3241 tcatgaacaa gtgaaagaat acttcccaga gattattgat gtggaattca cagtgaatat
     3301 ggaaacgtta cttgataaga ttgcagaagg cgacattaca tggagaaaag taatcgacgg
     3361 tttctttagt agctttaaac aagatgttga acgtgctgaa gaagagatgg aaaagattga
     3421 aatcaaagat gagccagccg gtgaagactg tgaagtttgt ggttctccta tggttataaa
     3481 aatggggcgt tatggtaagt ttatggcttg ctcaaacttc ccggattgtc gtaatacaaa
     3541 agcgatagtt aagtctattg gtgttaaatg tccaaaatgt aatgatggtg acgtcgtaga
     3601 aagaaaatct aaaaagaatc gtgtctttta tggatgttcg aaatatcctg aatgcgactt
     3661 tatctcttgg gataagccga ttggaagaga ttgtccaaaa tgtaaccaat atcttgttga
     3721 aaataaaaaa ggcaagacaa cacaagtaat atgttcaaat tgcgattata aagaggcagc
     3781 gcagaaataa tatttttatt tcctagagac attttaagat tgttaaatag aatcattagt
     3841 gaatcttatt ttaaagatag taatggatta atctaaataa gtgcggataa tattaacata
     3901 acaacataat taaaagacat aaatgacaat aaaaggagta tagaaatgac tcaaactgta
     3961 aatgtaatag gtgctggtct tgccggttca gaagcggcat atcaattatc tgaaagagga
     4021 attaaagtta atctaataga gatgagacct gttaaacaaa caccagcgca ccatactgat
     4081 aaatttgcgg aacttgtatg ttccaattca ttacgcggaa atgctttaac taatggtgtg
     4141 ggtgttttaa aagaagaaat gagaagattg aattctataa ttattgaagc ggctgataag
     4201 gcacgagttc cagctggtgg tgcattagca gttgatagac acgatttttc aggttatatt
     4261 actgaaacac ttaaaaatca tgaaaatatc acagttatta atgaagaaat taatgccatt
     4321 ccagatggat acacaattat cgcaacagga ccacttacta cagaaaccct tgcacaagaa
     4381 atagtggaca ttactggtaa agatcaactt tatttctatg atgcggctgc tccaattatt
     4441 gaaaaagaat ctattgatat ggataaagtt tacttaaagt cccgttatga taaaggtgaa
     4501 gctgcatatt taaactgtcc tatgactgag gatgaattta atcgctttta tgatgcagta
     4561 ttagaagctg aagctgcgcc tgtaaattca tttgaaaaag aaaaatattt cgagggttgt
     4621 atgccttttg aagtaatggc agaacgcggg cgcaagacat tactatttgg accaatgaaa
     4681 ccagtaggat tagaagatcc aaagactggg aaacgtcctt atgcggtggt tcaattaaga
     4741 caagatgacg ctgctggcac actctacaat attgtcggct tccaaacaca tttaaaatgg
     4801 ggagctcaaa aagaagtcat taaattaatt ccaggtttag agaatgttga tattgtcaga
     4861 tatggtgtga tgcatagaaa taccttcatt aactcaccgg acgtattaaa cgagaaatat
     4921 gaattgattt cacaaccaaa catacagttt gcgggccaaa tgactggtgt tgaaggttat
     4981 gtagaaagcg cagctagcgg cttagttgca ggcatcaatc ttgcgcataa aatattaggc
     5041 aaaggtgagg tagtatttcc gagagaaact atgattggaa gtatggctta ctatatttct
     5101 catgctaaaa acaataagaa tttccaacct atgaatgcta acttcgggtt attaccatct
     5161 ttagaaacta gaattaaaga taaaaaagaa cgctatgaag cacaagctaa tagagctttg
     5221 gattacttag aaaatttcaa aaaaacttta taaaatagtt agaaagacta gatatgctat
     5281 tcattcttaa gtcatcaacg agtaagtaat gactttctaa atggaaaata cttatcctag
     5341 tctttttaat tttggaattg ttacgtattt ctgacaattt agaattcgta ttcaaataaa
     5401 tatctaaata aataacacgc aataagttga ttgatgtaac atgtaagtga atgttttaaa
     5461 taaagtttat ttaaaagaca atgaaagaat aaatagtaag gctattaata aagactttta
     5521 gtaattaatt tacaaaagag gtattctaat taacaggttt tccgattagt tacaattatt
     5581 taattctcaa aagatttaga attgattatc aaattactgt aagccctttg ctgtatatgc
     5641 tacaattctt attgatggag ggtaaatgta ttgaatcata ttcaagaagc atttttaaat
     5701 acattgaaag ttgaacgaaa tttttcagaa cacacattga aatcatatca agatgactta
     5761 attcagttta atcaattttt agaacaggaa catttacagt tgaatacttt tgaatacaga
     5821 gatgctagaa attatttgag ctatttatat tcaaatcatt tgaaaagaac atctgtttct
     5881 cgtaaaatct caacgttaag aactttctat gaatattgga tgacgcttga tgagaacatt
     5941 attaatccat ttgttcaatt agtacatccg aaaaaagaaa aatatcttcc gcaattcttt
     6001 tacgaagaag aaatggaagc gttatttaaa actgtagaag aggacacttc aaaaagttta
     6061 cgggatcgag ttattcttga attgttatat gctacaggta tccgtgtttc ggaattagtt
     6121 aatattaaaa aacaagatat agatttttac gcgaatggtg ttaccgtatt aggaaaaggg
     6181 agcaaagagc gctttgtacc gtttggtgct tattgtagac aaagcatcga aaattattta
     6241 gaacatttca aaccaattca gtcatgcaat catgattttc ttattgtaaa tatgaagggt
     6301 gaagcaatca ctgaacgcgg tgtacgatat gttttaaatg atattgttaa acgaacagca
     6361 ggcgtaagtg agattcatcc ccacaagctc agacatacat ttgcaacgca tttattgaat
     6421 caaggtgcag atctaagaac agtacaatcg ttattaggtc atgttaattt gtcaacaact
     6481 ggtaaatata cacacgtatc taaccaacaa ttaagaaaag tgtatctaaa tgcacatcct
     6541 cgagcgaaaa aggagaatga aacatgagta atacaacatt acatgcaaca acaatttatg
     6601 ctgtaagaca taatgggaaa gcagctatgg ctggagatgg gcaagtaacg cttggtcaac
     6661 aagtcatcat gaaacaaacg gcaagaaaag tgcgtcgttt atatgaaggt aaagtgttag
     6721 ctggtttcgc aggtagtgta gcagatgcgt ttacgttatt tgaaaaattc gaaacaaaat
     6781 tacaacagtt tagtggtaac ttagaaagag ctgctgttga attggcacaa gaatggcgag
     6841 gcgataaaca attacgtcaa ttagaagcta tgctaattgt aatggataaa gatgctattt
     6901 tagttgtcag tggaactggc gaagttattg ccccagatga tgaccttatc gctattggat
     6961 caggaggcaa ctatgcatta agcgcaggac gtgcattgaa acgccatgca tcgcatttgt
     7021 ctgctgaaga aatggcatat gagagcttga aagtagcggc tgatatttgt gtctttacca
     7081 acgataatat tgttgtcgaa acactataat aatcagagca tgataaataa ttatgagcaa
     7141 ttaattttag ttaaaagacg gaggaatgaa attaatggat acagctggaa taagattaac
     7201 tccaaaagaa atcgtatcta aattaaatga atacatcgtt ggacaaaatg atgctaaacg
     7261 taaagtggca attgccctac gtaatcgata cagaagaagt ttattggatg aggaatcaaa
     7321 gcaagaaatt tcacctaaaa atattttgat gattggacca accggtgttg gtaaaactga
     7381 aattgcaaga agaatggcca aagttgtcgg cgcaccattt ataaaagtag aagctactaa
     7441 atttactgaa gtaggttatg taggacgaga tgttgaaagt atggttagag atcttgttga
     7501 tgtttcagta agattagtca aggcgcagaa aaaatcattg gtacaagatg aagcaacagc
     7561 taaggccaat gaaaaacttg ttaagttatt agttccaagt atgaaaaaga aagcgtctca
     7621 aacgaataat cctttagagt cacttttcgg aggtgcaatt ccaaatttcg gacaaaataa
     7681 cgaagatgaa gaagaaccac ctactgagga aattaaaaca aaacgttctg aaattaagag
     7741 acaattagaa gaaggaaaac ttgaaaaaga aaaggtaaga attaaagtcg aacaagatcc
     7801 tggtgcttta ggtatgctag gtacaaatca aaatcagcaa atgcaagaga tgatgaatca
     7861 attaatgcct aaaaagaaag ttgagcgaga agttgctgtt gagacggcaa ggaaaatctt
     7921 agctgatagt tatgcggatg aactaattga tcaagaaagc gctaaccaag aagcacttga
     7981 attagcagaa caaatgggta tcatctttat agatgaaatc gacaaagttg cgacgaataa
     8041 tcataatagt ggtcaagatg tctcaagaca aggtgtgcaa agagatattt tacctatact
     8101 tgaaggtagc gttattcaaa ccaaatatgg tactgtgaat actgaacata tgctgtttat
     8161 aggtgctgga gctttccatg tatctaagcc gagtgacttg ataccagaat tgcaaggtcg
     8221 ttttccgatt agagttgaac ttgatagttt agcggtagaa gattttgtaa gaattttgac
     8281 agaaccaaaa ttgtcattaa ttaaacaata tgaagcattg cttcaaacag aagaagttac
     8341 tgtaaacttt accgatgaag caattactcg tttagctgag attgcttatc aagtaaatca
     8401 agatacagac aacattggtg cacgtcgact tcatacaatt ttagaaaaga tgttagaaga
     8461 tttatcattc gaagcaccaa gtatgccgaa tgcagttgta gatattaccc cacaatatgt
     8521 tgatgataaa ttaaaatcaa tttcaacaaa taaagattta agtgcattta ttctataaaa
     8581 atatacaaaa ggagaaaaat tcatgagctt attatctaaa acgagagagt taaacacgtt
     8641 acttcaaaaa cacaaaggta ttgcggttga ttttaaagat gtagcacaaa cgattagtag
     8701 cgtaactgta acaaatgtat ttattgtatc gcgtcgaggt aaaattttag gatcgagtct
     8761 aaatgaatta ttaaaaagtc aaagaattat tcaaatgttg gaagaaagac atattccaag
     8821 tgaatataca gaacgattaa tggaagttaa acaaacagaa tcaaatattg atatcgacaa
     8881 tgtattaaca gttttcccac ctgaaaacag agaattattc atagatagtc gtacaactat
     8941 cttcccaatt ttaggcggag gagaaagatt aggtacatta gtacttggtc gagtacacga
     9001 tgactttaat gaaaatgatt tggtactagg tgaatatgct gctacagtta ttggtatgga
     9061 aatcttacgt gagaagcata gtgaagtaga aaaagaagcg cgcgataaag ctgctattac
     9121 aatggcaatt aattcattat cttattctga aaaagaagca attgaacata tctttgaaga
     9181 acttggcggt acagaaggcc tattaatcgc atcaaaagtt gcagatagag ttggtattac
     9241 tagatctgta attgtaaatg cactacgtaa attagaaagt gctggtgtaa ttgaatcacg
     9301 ttctttagga atgaaaggta ctttcattaa agttaaaaaa gaaaaattct tagatgaatt
     9361 agaaaaaagt aagtaaaacg atgagtctgg gacataattc aatgtcctag tcgtaacgat
     9421 gttatcttgg cagtagtcaa cgaaatgact ttgatgatta tgttgaagtc atttctttat
     9481 ttttgtctaa ttttcaatca aattgatatt ccgcattaac atgatagaat tacctttaaa
     9541 tttatgaaaa aatgcttgaa aaacatttgc tttagacacg gcattatgtt atatttattt
     9601 tcggctataa agccaataca cacatatcaa acgtgattgt gtaaagggtg caatatttgt
     9661 atattgtctt tacaatagtt tgctatggag gtaattaacc aataggagga atttataatg
     9721 gcagtaattt caatgaaaca attactagaa gcgggtgttc acttcggtca ccaaacacgt
     9781 cgttggaacc caaaaatgaa aaaatatatc ttcactgaga gaaatggtat ttatatcatc
     9841 gacttacaaa aaacagtgaa aaaagtagac gaggcataca acttcttgaa acaagtttca
     9901 gaagatggtg gacaagtctt attcgtagga actaaaaaac aagcacaaga atcagttaaa
     9961 tctgaagcag aacgtgctgg tcaattctac attaaccaaa gatggttagg tggattatta
    10021 actaactata aaacgatctc aaaacgaatc aaacgtattt ctgaaattga aaaaatggaa
    10081 gaagatggtt tattcgaagt attacctaaa aaagaagtag tagaacttaa aaaagaatac
    10141 gaccgtttaa tcaaattctt aggcggaatt cgtgatatga aatcaatgcc tcaagcatta
    10201 ttcgtagttg acccacgtaa agagcgtaat gcaattgctg aagctcgtaa attaaatatt
    10261 cctatcgtag gtatcgttga cactaactgt gatcctgacg aaattgacta cgttatccca
    10321 gcaaacgacg atgctatccg tgcggttaaa ttattaactg ctaaaatggc agatgcaatc
    10381 ttagaaggtc aacaaggcgt ttctaatgaa gaagtagctg cagaacaaaa catcgattta
    10441 gatgaaaaag aaaaatcaga agaaacagaa gcaactgaag caactgaaga ataatcaact
    10501 gttgaatctg acttagatat agtttaaatg ggtgataaga tattaatgct tatcaccttt
    10561 tttaaaaaga aaatcaaggc aaattacaaa tattcaatta gagtattggc aatcttgcct
    10621 ataataatgc taaaattata atatataaaa tgataactta ttggaggaat aatgaatggc
    10681 aactatttca gcaaaacttg ttaaagaatt acgtgaaaaa actggcgcgg gtatgatgga
    10741 ttgtaaaaaa gcgctgactg aaactgatgg tgacatcgat aaagcgattg attacctacg
    10801 tgaaaaaggt attgctaaag cagctaaaaa agcagaccgt attgcggctg aaggtttagt
    10861 acatgtagaa actaaaggta acgacgcagt tatcgttgaa atcaactctg aaacagactt
    10921 tgttgctcgt aacgaaggtt tccaagagtt agttaaagaa atcgctaatc aagtattaga
    10981 tacaaaagct gaaactgttg aagctttaat ggaaacaact ttaccaaatg gtaaatcagt
    11041 tgatgaaaga attaaagaag caatttcaac aatcggtgaa aaattaagtg ttcgtcgttt
    11101 tgctatcaga actaaaactg ataacgatgc tttcggcgct tacttacaca tgggtggacg
    11161 cattggtgta ttaacagttg ttgaaggttc aactgacgaa gaagcagcaa gagacgttgc
    11221 tatgcatatc gctgcaatca accctaaata tgtttcttct gaacaagtta gcgaagaaga
    11281 aatcaaccac gaaagagaag ttttaaaaca acaagcatta aatgaaggta aaccagaaaa
    11341 catcgttgaa aaaatggtgg aaggacgttt acgtaaatac ttacaagaaa tttgtgctgt
    11401 agatcaagac ttcgttaaaa accctgatgt aacagttgaa gctttcttaa aaacaaaagg
    11461 tggaaaactt gttgacttcg tacgctatga agtaggcgaa ggtatggaaa aacgcgaaga
    11521 aaactttgcg gatgaagtta aaggacaaat gaaataatct gtcataaagt aaaacaagga
    11581 agaagacacc tttaatgttg ctttattaaa atgtaaatca ttctaataaa acgacaactg
    11641 tgtcttcttt acttgtatat gttacatata ttcacgatag agaggataag aaaatggctc
    11701 aaatttctaa atataaacgt gtagttttga aactaagtgg tgaagcgtta gctggagaaa
    11761 aaggatttgg cataaatcca gtaattatta aaagtgttgc tgagcaagtg gctgaagttg
    11821 ctaaaatgga ctgtgaaatc gcagtaatcg ttggtggcgg aaacatttgg agaggtaaaa
    11881 caggtagtga cttaggtatg gaccgtggaa ctgctgatta catgggtatg cttgcaactg
    11941 taatgaatgc cttagcatta caagatagtt tagaacaatt ggattgtgat acacgagtat
    12001 taacatctat tgaaatgaag caagtggctg aaccttatat tcgtcgtcgt gcaattagac
    12061 acttagaaaa gaaacgcgta gttatttttg ctgcaggtat tggaaaccca tacttctcta
    12121 cagatactac agcggcatta cgtgctgcag aagttgaagc agatgttatt ttaatgggca
    12181 aaaataatgt agatggtgta tattctgcag atcctaaagt aaacaaagat gcggttaaat
    12241 atgaacattt aacgcatatt caaatgcttc aagaaggttt acaagtaatg gattcaacag
    12301 catcctcatt ctgtatggat aataacattc cgttaactgt tttctctatt atggaagaag
    12361 gaaatattaa acgtgctgtt atgggtgaaa agataggtac gttaattaca aaataaattt
    12421 agaggtgtaa aataatgagt gacattatta atgaaactaa atcaagaatg caaaaatcaa
    12481 tcgaaagctt atcacgtgaa ttagcgaaca tcagtgcagg aagagctaat tcaaatttat
    12541 taaatggcgt aacagttgat tactatggtg caccaacacc tgtacaacaa ttagcaagca
    12601 tcaatgttcc agaagcacgt ttacttgtta tttctccata cgacaaaact tctgtagctg
    12661 acatcgaaaa agcgataata gcagctaact taggtgtcaa cccaacaagt gatggtgaag
    12721 tgatacgtat tgctgtacct gccttaacag aagaacgtag aaaagagcgc gttaaagatg
    12781 ttaagaaaat tggtgaagaa gctaaagtat ctgttcgaaa tattcgtcgt gatatgaatg
    12841 atcagttgaa aaaagatgaa aaaaatggcg acattactga agatgagttg agaagtggta
    12901 ctgaagatgt tcagaaagca acagacaatt caataaaaga aattgatcaa atgattgctg
    12961 ataaagaaaa agatattatg tcagtataaa actaatatac aatgacatat taaaatgcca
    13021 gtattaaacg ataatgtaac atttaaaatg ggcatgttta attaaatcaa agatgcatgt
    13081 gataatttaa attcacaatg agcataaaaa tggtgtttaa acaagttaat taaacatata
    13141 ctttataaat aataggcatt aggtatattg atataataaa gttatgtaat ttttaacctc
    13201 agtatgtatg tcacatttct ggtgtaaact gtaccgagtc agactttggt acagtttttt
    13261 tatttgctta ttcaatgcat taaatgagta tgataaaatg ataatgattg tttagtaact
    13321 tatactatat gacagagatg atcaggctcg gaggaaagac catgtttaaa aagctaataa
    13381 ataaaaagaa cactataaat aattataatg aagaattaga ctcgtctaat atacctgaac
    13441 atatcgctat tattatggat ggtaatgggc gatgggctaa gaagcgaaaa atgcctagaa
    13501 ttaaaggtca ttacgaaggt atgcaaacaa taaaaaaaat tactagggta gctagtgata
    13561 ttggtgttaa gtacttaact ttatatgcct tttccactga aaattggtca agacctgaaa
    13621 gtgaagtaaa ttatattatg aatttgcctg tcaatttctt aaagacattt ttaccggaac
    13681 taattgaaaa aaatgtcaaa gttgaaacaa ttggatttac tgataagttg ccaaaatcaa
    13741 cgatagaagc aataaataat gctaaagaaa agacagctaa taataccggc ttaaaattaa
    13801 tatttgcaat taattatggt ggcagagcag aacttgttca tagtattaaa aatatgtttg
    13861 acgagcttca tcaacaaggt ttgaatagtg atatcataga tgaaacatat ataaacaatc
    13921 atttaatgac aaaagactat cctgatccag agttgttaat tcgtacttca ggagaacaaa
    13981 gaataagtaa tttcttgatt tggcaagttt cgtatagtga atttatcttt aatcaaaaat
    14041 tatggcctga ctttgacgaa gatgaattaa ttaaatgtat taaaatttat cagtcacgtc
    14101 aaagacgctt tggcggattg agtgaggagt agtatagtat gaaagttaga acgctgacag
    14161 ctattattgc cttaatcgta ttcttgccta tcttgttaaa aggcggcctt gtgttaatga
    14221 tatttgctaa tatattagca ttgattgcat taaaagaatt gttgaatatg aatatgatta
    14281 aatttgtttc agttcctggt ttaattagtg cagttggtct tatcatcatt atgttgccac
    14341 aacatgcagg gccatgggta caagtaattc aattaaaaag tttaattgca atgagcttta
    14401 ttgtattaag ttatactgtc ttatctaaaa acagatttag ttttatggat gctgcatttt
    14461 gcttaatgtc tgtggcttat gtaggcattg gttttatgtt cttttatgaa acgagatcag
    14521 aaggattaca ttacatatta tatgcctttt taattgtttg gcttacagat acaggggctt
    14581 acttatttgg taaaatgatg ggtaaacata agctttggcc agtaataagt ccgaataaaa
    14641 caatcgaagg attcataggt ggcttgttct gtagtttgat agtgcctctt gcaatgttat
    14701 attttgtaga tttcaatatg aatgtatgga tattacttgg agtgacattg attttaagtt
    14761 tatttggtca attaggtgat ttagtggaat caggatttaa gcgtcatttc ggcgttaaag
    14821 actcaggtcg aatactacct ggacacggtg gtattttaga ccgatttgac agttttatgt
    14881 ttgtgttacc attattaaat attttattaa tacaatctta atgctgagaa caaatcaata
    14941 aacgtaaaga ggagttgctg agataattta atgaatctca gaactccttt tgaaaattat
    15001 acgcaatatt aactttgaaa attagacgtt atattttgtg atttgtcagt atcatattat
    15061 aatgacttat gttacgtata cagcaatcat ttttaaaata aaagaaattt ataaacaatc
    15121 gaggtgtagc gagtgagcta tttagttaca ataattgcat ttattattgt ttttggtgta
    15181 ctagtaactg ttcatgaata tggccatatg ttttttgcga aaagagcagg cattatgtgt
    15241 ccagaatttg cgatcggtat ggggccgaaa atttttagtt ttagaaaaaa tgaaacactt
    15301 tacactatta ggttattgcc tgttggtgga tatgttcgta tggcaggaga tggcttagaa
    15361 gagccaccag tcgagcctgg tatgaacgtt aaaattaaac tgaatgaaga aaatgaaata
    15421 acacatatca tattagatga tcatcataag tttcaacaaa ttgaagcgat cgaagttaaa
    15481 aaatgtgatt ttaaagatga cttattcata gaaggtatca ctgcttatga taatgaaaga
    15541 catcatttta aaattgctag aaagtctttc tttgttgaaa atggtagctt agttcaaatt
    15601 gctccgagag acagacaatt tgcacataaa aagccatggc cgaaattttt aacattattt
    15661 gcgggaccgt tatttaactt tatattagct ttagttctat ttattggtct tgcatattat
    15721 caaggtacac ctacttctac tgtagaacaa gtcgcagata agtatccagc tcaacaagca
    15781 ggcatacaaa aaggcgataa aattgtccaa attggcaaat ataaaatatc tgaatttgat
    15841 gatgttgata aggcgttaga taaagttaaa gataataaga cgactgttaa atttgaacgt
    15901 gatggtaaaa caaagtcagt tgaattaaca cctaaaaaga ccgaaagaaa actgactaaa
    15961 gtaagttcag agacgaagta tgttctcgga ttccaaccag cgagtgaaca tacactcttc
    16021 aaaccaattg tatatggatt taaaagcttt ttaatcggta gtacttatat tttttcagct
    16081 gtagtaggta tgttggctag tatatttacg ggcggattct cgtttgatat gttaaatggt
    16141 ccggttggta tttatcataa cgtcgactca gttgttaaag cgggtatcat tagcttaatt
    16201 ggatacactg cgttattaag tgtaaactta ggtattatga atttaattcc tattcctgca
    16261 ctagacggtg gtcgtatttt atttgttata tatgaagcga ttttcagaaa accagttaat
    16321 aaaaaagcgg aaacaacgat tatcgctatt ggtgccattt tcatggtcgt tattatgata
    16381 ttagtaacgt ggaatgatat tcgacgatat ttcttataat ttaggaggat aaataattat
    16441 gaagcaatcc aaagttttta taccaacgat gcgtgacgtg ccatcagaag cagaagcaca
    16501 aagtcatcgt ttattattga aagcgggttt gataaaacaa agtacaagtg ggatttatag
    16561 ttatttaccg ctagcaacac gtgtgttaaa taatattact gcaattgtgc gacaagaaat
    16621 ggaacgtatc gattctgttg aaattttaat gccagcgtta caacaagctg aattatggga
    16681 agaatcagga cgttggggtg catatggccc agaattaatg cgtttacaag atagacatgg
    16741 aagacaattt gcattaggtc caacacatga agaattagtt acatcaatag taagaaatga
    16801 attgaaatca tacaaacaat taccgatgac attattccaa attcaatcta aattccgtga
    16861 tgaaaagaga ccacgttttg gtttacttcg tggtcgtgaa tttattatga aagatgcgta
    16921 ttcattccat gctgacgagg catcattaga tcaaacgtat caagatatgt atcaagcgta
    16981 tagccgtatt tttgagagag ttggcattaa cgcaagacca gtagttgcag attcaggtgc
    17041 tattggcggt agccatacac atgaatttat ggcattaagt gctatcggtg aggatacaat
    17101 cgtttacagt aaagaaagtg actatgctgc taacatcgaa aaagcagaag tcgtttacga
    17161 accaaatcat aagcatacta ctgtgcaacc tatagaaaaa attgaaacac caaatgttaa
    17221 gactgcgcaa gaattggcag acttcttagg tagaccagta gatgaaatcg ttaaaacgat
    17281 gattttcaaa gttgatggcg aatatattat ggttttagtg cgtggacatc atgaaattaa
    17341 tgacattaaa ttaaaatctt atttcggcac agataatatt gaattagcaa cacaagacga
    17401 aattgttaat ttagttggtg caaatcctgg ttcactaggt cctgtaattg ataaagagat
    17461 caaaatttat gcagataatt ttgtgcaaga tttaaataat ttagttgtcg gggctaacga
    17521 agatggttat cacttaatta atgtaaatgt aggtagagac ttcaacgttg atgaatatgg
    17581 cgatttccgt tttattttag aaggcgaaaa gttaagtgat ggttcaggcg ttgcacattt
    17641 tgctgaagga attgaagttg gtcaagtatt caaattgggt actaagtatt cagaatcaat
    17701 gaatgctaca ttcttagata atcaaggaaa agcccaaccg ttaattatgg gttgttacgg
    17761 tattggaatt tctagaacgc taagtgcgat tgttgaacaa aatcacgatg ataatggtat
    17821 tgtttggcct aaatcagtta ctccatttga tttacattta atttctatta atcctaagaa
    17881 agatgatcaa cgagaactag cggatgcact atatgctgaa tttaatacta aatttgatgt
    17941 gttgtacgat gatcgtcagg aacgtgctgg tgtcaaattt aatgatgccg atttaattgg
    18001 tttaccactg cgaattgttg ttggtaaacg tgcatcggaa ggtattgtag aagttaaaga
    18061 acgtttaaca ggtgatagcg aagaagtcca cattgatgac ttaatgactg tcattacaaa
    18121 taaatatgat aatttaaaat aattaagatc gaatgaatta taagagtagg aaaaagctga
    18181 aagaaatcag atgcttatgt cctgctctta ttatttttga tataatgatt attcgatgaa
    18241 aaatgactga agacatagta taattaaaga taaatttgtt ttaacaatat tatgattagc
    18301 caaatataaa gcatttaatt ttctatcatt actatgctca cataatctaa atattgttcg
    18361 aacacgtaaa agtaatttct atttaaggtg gtaattgtct tggcaatgac agagcaacaa
    18421 aaatttaaag tgcttgctga tcaaattaaa atttcaaatc aattagatgc tgaaatttta
    18481 aattcaggtg aactgacacg tatagatgtt tctaacaaaa acagaacatg ggaatttcat
    18541 attacattac cacaattctt agctcatgaa gattatttat tatttataaa tgcaatagag
    18601 caagagttta aagatatcgc caacgttaca tgtcgtttta cggtaacgaa tggcacgaat
    18661 caagatgaac atgcaattaa atactttggg cactgtattg accaaacagc tttatctcca
    18721 aaagttaaag gtcaattgaa acagaaaaag cttattatgt ctggaaaagt attaaaagta
    18781 atggtatcaa atgacattga acgtaatcat tttgataagg catgcaatgg aagtcttatc
    18841 aaagcgttta gaaattgtgg ttttgatatc gataaaatca tattcgaaac aaatgataat
    18901 gatcaagaac aaaacttagc ttctttagaa gcacatattc aagaagaaga cgaacaaagt
    18961 gcacgattgg caacagagaa acttgaaaaa atgaaagctg aaaaatcgaa acaacaagat
    19021 aacaacgaaa gtgctgtcga taagtgtcaa attggtaagc cgattcaaat tgaaaatatt
    19081 aaaccaattg aatctattat tgaggaagag tataaagttg caatagaggg tgtcattttt
    19141 gatattaact taaaagaact taaaagtggt cgtcatatcg tagaaattaa agtgactgac
    19201 tatacggact ctttagtttt aaaaatgttt actcgtaaaa acaaagatga tttagaacat
    19261 tttaaagcgc taagtgttgg taaatgggtt agggctcaag gtcgtattga agaagataca
    19321 tttattagag atttagttat gatgatgtct gatattgaag agattaaaaa agcgacaaaa
    19381 aaagataagg ctgaagaaaa acgtgtagaa ttccacttac atactgcaat gagtcaaatg
    19441 gatggtatac caaatattgg tgcgtatgtt aaacaggcag cagactgggg acatccagcc
    19501 attgcggtta cagaccataa tgttgtgcaa gcatttccag atgctcacgc agcagcggaa
    19561 aaacatggta ttaaaatgat atatggtatg gaaggtatgt tagttgatga tggtgttccg
    19621 attgcataca aaccacaaga tgtcgtatta aaagatgcta cttatgttgt attcgacgtc
    19681 gagacaactg gtttatcaaa tcagtatgat aaaatcatcg agcttgcagc tgtgaaagtt
    19741 cataacggcg aaatcatcga taagtttgaa aggtttagta atccgcatga acgattatcg
    19801 gaaacgatta tcaatttgac gcatattact gatgatatgt tagtagatgc ccctgagatt
    19861 gaagaagtac ttacagaatt taaagaatgg gttggcgatg cgatattcgt agcacataat
    19921 gcttcgtttg atatgggctt catcgacacg ggatatgaac gtcttggatt tggaccatct
    19981 acgaatggtg ttatcgatac attagaatta tctcgtacga ttaatactga atatggtaaa
    20041 catggtttaa atttcttagc taaaaaatac ggtgtagaat taacgcaaca tcaccgtgcc
    20101 atttatgata cagaggcaac agcttacatt ttcataaaaa tggttcaaca aatgaaagaa
    20161 ttaggcgtat taaatcataa cgaaattaac aaaaaactca gtaatgaaga tgcatataaa
    20221 cgtgcaagac ctagtcatgt cacattaatt gtacaaaatc aacaaggtct taaaaatcta
    20281 tttaaaattg taagtgcatc attggtgaag tatttctacc gtacgcctcg aattccacgt
    20341 tcattgttag atgaatatcg tgagggatta ttggtaggta cagcgtgtga tgaaggtgaa
    20401 ttatttacgg cagttatgca gaaggaccag agccaagttg aaaaaattgc caaatattat
    20461 gattttattg aaattcaacc accggcactt tatcaagatt taattgatag agagcttatt
    20521 agagatactg aaacattaca tgaaatttat caacgtttaa ttcatgcagg tgacacagcg
    20581 ggtattcctg ttattgcgac aggaaatgca cactatttgt ttgaacatga tggcatcgca
    20641 cgtaaaattt taatagcatc acaacccggc aatccactta atcgctcaac tttaccggaa
    20701 gcacatttta gaactacaga tgaaatgtta aacgagtttc attttttagg tgaagaaaaa
    20761 gcgcatgaaa ttgttgtgaa aaatacaaac gaattagcag atcgaattga acgtgttgtt
    20821 cctattaaag atgaattata cacaccgcgt atggaaggtg ctaacgaaga aattagagaa
    20881 ctaagttatg caaatgcgcg taaactgtat ggtgaagatc tgcctcaaat cgtaattgat
    20941 cgattagaaa aagaattaaa aagtattatc ggtaatggat ttgcggtaat ttacttaatt
    21001 tcgcaacgtt tagttaaaaa atcattagat gatggatact tagttggttc ccgtggttca
    21061 gtaggttcta gttttgtggc gacaatgact gagattactg aagtaaaccc gttaccgcca
    21121 cactatatat gtccgaactg taaaacgagt gaatttttca atgatggttc agtaggatca
    21181 ggattcgatt tacctgataa gacgtgtgaa acttgtggag cgccacttat taaagaagga
    21241 caagatattc cgtttgaaac atttttagga tttaaaggag ataaagttcc tgatatcgac
    21301 ttaaacttta gtggtgaata tcaaccaaat gcccataact acacaaaagt attatttggt
    21361 gaggataaag tattccgtgc aggtacaatt ggtactgttg ctgaaaagac tgcttttggt
    21421 tatgttaaag gctatttgaa tgatcaaggc atccacaaaa gaggtgctga aatagatcga
    21481 ctcgttaaag gatgtacagg tgttaaacgt acaactggac agcatccagg gggtattatt
    21541 gtagtacctg attacatgga tatttatgat tttacgccga tacaatatcc tgccgatgat
    21601 caaaattcag catggatgac gacacatttt gatttccatt ctattcatga taatgtatta
    21661 aaacttgata tacttggaca cgatgatcca acaatgattc gtatgcttca agatttatca
    21721 gggattgatc caaaaacaat acctgtagat gataaagaag ttatgcagat ttttagtaca
    21781 cctgaaagtt tgggtgttac tgaagatgaa attttatgta aaacaggtac atttggggta
    21841 ccagaattcg gtacaggatt cgtgcgtcaa atgttagaag atacaaagcc aacaacattt
    21901 tcagaattag ttcaaatctc aggattatct catgggacag atgtgtggtt aggtaatgct
    21961 caagaattaa ttaaaacagg tatatgtgat ttatcaagtg taattggttg tcgtgacgat
    22021 atcatggttt atttaatgta tgctggtcta gaaccatcaa tggcttttaa aataatggag
    22081 tcagtacgta aaggtaaagg tttaactgaa gaaatgattg aaacgatgaa agaaaatgaa
    22141 gtgccggatt ggtatttaga ttcatgtctt aaaattaagt acatgttccc taaagcccat
    22201 gcagcagcat acgttttaat ggcagtacgt atcgcatatt tcaaagtaca tcatccactt
    22261 tattactatg catcttactt tacaattcgt gcgtcagatt ttgatttaat cacgatgatt
    22321 aaagataaaa caagcatacg aaatactgta aaagacatgt attctcgcta tatggatcta
    22381 ggtaaaaaag aaaaagacgt attaactgtc ttggaaatta tgaatgaaat ggcgcatcgg
    22441 ggttatcgaa tgcaaccgat tagtttagaa aagagtcaag cgttcgaatt tatcattgaa
    22501 ggcgaaacac ttattccgcc gttcatatca gtgcctgggc ttggcgaaaa cgttgcgaaa
    22561 cgaattgttg aagctcgtga cgatggacca tttttatcaa aagaagattt aaacaaaaaa
    22621 gctggattat ctcagaaaat tattgagtat ttagatgagt taggctcatt accgaattta
    22681 ccagataaag ctcaactttc gatattcgat atgtaaaatg aaataatcaa ggtatttatt
    22741 taattcgtat ggcgtagtta aagaaataca aaattgttgc tggacacaaa gttatacctg
    22801 tatttctttt caacgtctta cgagtctatt cgaatgtaat gatgaaataa atgaacaaac
    22861 ttttacaaga atctctgatt aatagtgaag tcatttgttt caagcataaa cttatgctat
    22921 aattaagttg cttaaaaatt agtgaactca ggcagaagag tgggagattc ccgctctttt
    22981 ctatttgcca aaaagggagg cctgtatgag taaaattaca gaacaagtag aagtgattgt
    23041 taaaccaatt atggaagact tgaattttga acttgtagac gttgaatatg tcaaagaggg
    23101 tagagatcat tttcttagaa tctctattga taaagaaggt ggcgtagatt taaatgattg
    23161 tacgctagct tctgaaaaaa taagtgaagc tatggatgca aatgatccta ttcctgaaat
    23221 gtattattta gacgtagcgt cacctggtgc agaacgtcca attaaaaaag aacaagattt
    23281 ccaaaatgca ataactaaac ctgtatttgt ttctttatat gtaccaattg aaggtgaaaa
    23341 ggaatggtta ggcattttac aagaagtcaa taatgaaaca attgtagtac aagttaaaat
    23401 caaagcaaga acgaaagata tagagatacc gagagacaaa atagcaaaag cacgtcacgc
    23461 agttatgatt taacgtgatg aggaggaaaa aacgtgtcaa gtaatgaatt attattagct
    23521 actgagtatt tagaaaaaga aaagaagatt cctagagcag tattaattga tgctattgaa
    23581 gcagctttaa ttactgcata caaaaagaat tatgatagtg caagaaatgt ccgtgtggaa
    23641 ttaaatatgg atcaaggtac tttcaaagtt atcgctcgta aagatgttgt tgaagaagta
    23701 tttgacgaca gagatgaagt ggatttaagt acagcgcttg ttaaaaaccc tgcatatgaa
    23761 attggtgata tatacgaaga agatgtaaca cctaaagatt ttggtcgtgt aggtgctcaa
    23821 gcagcgaaac aagcagtaat gcaacgtctt cgtgatgctg aacgtgaaat tttatttgaa
    23881 gaatttatag acaaagaaga agacatactt actggaatta ttgaccgtgt tgaccatcgt
    23941 tatgtatatg tgaatttagg tcgtattgaa gctgttttat ctgaagcaga aagaagtcct
    24001 aacgaaaaat atattcctaa cgaacgtatc aaagtatatg ttaacaaagt ggaacaaacg
    24061 acaaaaggtc ctcaaatcta tgtttctcgt agccatccag gtttattaaa acgtttattt
    24121 gaacaagaag ttccagaaat ttacgatggt actgtaattg ttaaatcagt agcacgtgaa
    24181 gctggcgatc gctctaaaat tagtgtcttc tctgaaaaca atgatataga tgctgttggt
    24241 gcatgtgttg gtgccaaagg cgcgcgtgtt gaagctgttg ttgaagagct aggtggcgaa
    24301 aaaatcgata tcgttcaatg gaatgaagat ccaaaagtat ttgtaaaaaa tgctttaagc
    24361 ccttctcaag tgttagaagt tattgttgat gaaacaaatc aatctacagt agttgttgtt
    24421 cctgattatc aattgtcatt agcgattggt aaaagaggac aaaacgcacg tctagctgct
    24481 aaattaaccg gctggaaaat tgatattaaa tcagaaacag atgcgcgtga agcgggtatc
    24541 tatccagtag ttgaagctga aaaagtaact gaagaagatg ttgctttaga agatgctgac
    24601 acaacagaat caaccgaaga ggtaactgat gtttcagttg aaacaaatgt agagaaagaa
    24661 tctgaataat aggttggagt gaagtatcta tgaaaaagaa aaaaattccg atgcgaaaat
    24721 gtattctttc aaatgaaatg catcccaaaa aagatatgat tcgtgttgtt gttaataaag
    24781 aaggcgaaat ctttgcggat gttactggaa agaaacaagg ccgtggcgcg tatgtttcta
    24841 aagatgttgc tatggttgaa aaagcacaac aaaaagaaat tttagaaaaa tattttaaag
    24901 catctaaaga gcaattggat ccggtttaca aagaaattat tagattaatt tatagagaag
    24961 agatcccaaa atgagtatag atcaaatatt aaacttttta ggattagcaa tgagagccgg
    25021 taaagtaaaa acaggtgaat cagtcattgt taatgaaatt aaaaaaggaa atttgaagct
    25081 cgttattgtt gcaaatgatg cgtctaataa tacagctaaa ttaattacag ataaatgtaa
    25141 gagttacaaa gttccattca gaaagtttgg aaatcgaaat gaattgggaa tagcacttgg
    25201 aaaaggtgag cgtgttaatg tagggattac tgacccaggc tttgctaaaa agttgctatc
    25261 aatgatagat gaatatcata aggagtgatt atatgagtaa acaaagaatt tacgaatatg
    25321 cgaaagaatt aaatctaaag agtaaagaga ttatagatga gttaaaaagc atgaatattg
    25381 aggtttcaaa tcatatgcaa gctttggaag atgaccaaat taaagcatta gataaaaagt
    25441 tcaaaaaaga acaaaagaac gacaataaac aaagcactca aaataatcac caaaaatcaa
    25501 acaatcaaaa tcaaaataaa gggcaacaaa aagataacaa aaagaatcaa caacaaaata
    25561 ataaaggcaa caaaggcaat aaaaagaata atagaaataa taagaaaaat aacaagaata
    25621 ataaaccaca aagtcaacca gctgctccaa aagaaatacc atcaaaagtg acatatcaag
    25681 aaggtattac agtaggcgaa tttgcggata aattaaatgt tgaatcatca gaaattatca
    25741 aaaaattatt cttacttggt attgttgcta atatcaatca atcattaaat caagaaacaa
    25801 tcgaattaat tgccgatgat tatggcgttg aggttgaaga agaagttgtg attaatgaag
    25861 aagatttatc aatctacttc gaagacgaaa aagatgatcc agaagcaatt gagagaccag
    25921 ctgttgtaac aattatggga catgttgacc atggtaaaac tactttatta gattcaattc
    25981 gtcatacaaa agttacagca ggtgaagcag gcggaatcac tcaacatatt ggtgcatatc
    26041 aaattgaaaa cgatggcaaa aaaatcactt tcttagatac accgggacat gctgcattta
    26101 caacgatgcg tgcgcgtggt gcacaagtaa cagatattac aattttagta gtagcagctg
    26161 acgatggtgt tatgccacaa acaattgaag caattaacca tgctaaagaa gcagaagtac
    26221 caattattgt tgcagtaaat aaaattgata aaccaacttc aaatcctgat cgagttatgc
    26281 aagaattaac tgaatatggt ttaattcctg aagattgggg cggcgaaaca attttcgttc
    26341 cactttctgc attaagtggt gatggtatcg acgatttatt agaaatgata ggattagttg
    26401 cagaagttca agaacttaaa gcaaatccta aaaaccgtgc tgttggtaca gttatcgaag
    26461 ctgaattaga taaatcacga ggtccttctg catcattatt agtacaaaac ggtacattaa
    26521 atgttggtga tgcgattgta gtgggtaata cttacggccg tatccgtgca atggttaatg
    26581 acttaggtca aagaatcaaa acggctggtc catcaacgcc tgttgaaatt acaggtatta
    26641 atgatgtgcc acaagctggg gatcgctttg ttgtatttag tgatgaaaaa caagctcgtc
    26701 gtattggtga atcaagacac gaagctagca ttgtacaaca acgtcaagaa agtaaaaatg
    26761 tttcattaga taacctgttt gaacaaatga aacaaggtga aatgaaagac ttaaacgtta
    26821 ttattaaagg tgatgttcaa ggttctgttg aagctttagc tgcatcatta atgaaaattg
    26881 atgttgaagg cgtaaatgtt cgtatcattc atacagcggt tggtgcgatt aacgagtcag
    26941 acgtgacact tgctaatgcc tcaaatggta ttatcattgg tttcaatgtt cgtccagaca
    27001 gtggtgcaaa acgtgctgca gaagctgaaa atgttgatat gcgtttacac agagttattt
    27061 ataatgttat cgaagaaatt gaatcagcga tgaaaggttt acttgatcca gaatttgaag
    27121 aacaagttat cggacaagct gaagttcgtc aaacattcaa agtttctaaa gttggtacta
    27181 ttgctggatg ttatgttact gaaggtaaaa ttacgcgaaa tgctggtgta cgtattattc
    27241 gtgatggtat tgttcaatat gaaggcgaat tagatacact taaacgtttc aaagatgatg
    27301 ctaaggaagt tgcaaaaggt tatgaatgtg gtattacaat tgaaaactac aatgacctta
    27361 aagaaggcga tgttatcgaa gcatttgaaa tggttgaaat taagcgttaa ttaaataaat
    27421 tacaagctaa aagtttagtt aagattgata tgctccctat aaatattaca ctttttaagt
    27481 gtttacttta tagggagcat atttgataca agcttttggt tttttattag aatagattac
    27541 ctattaaaag ttacgttata tagacatgat ttttgtataa aattttgtgg tgacctagaa
    27601 tgctttttaa tgacaaaata taatgtcgac tattattgga aaattttctg ttgaaatgcc
    27661 tatcttacgg caaactttat ttgattttat aggcttaatt tattaaaata acgtgtgagc
    27721 taaaataatt gtttaagcat tgttacacta aaaaatgcaa ataacaattg aacttaaaga
    27781 taaagaggtg acaagaatga gcagtatgag agcagagcgt gttggtgaac aaatgaagaa
    27841 ggaattaatg gatatcatca acaataaagt caaagatcct cgagttggtt ttattacaat
    27901 tacagatgtt gttttaacaa atgatttatc gcaggctaaa gtatttttaa ctgtattagg
    27961 taacgataaa gaagtagaaa atacatttaa agcacttgat aaagcaaaag gcttcattaa
    28021 gtctgaatta ggttctagaa tgcgattacg tattatgccg gaattaatgt atgaatatga
    28081 tcaatcaatc gaatatggta ataaaattga acgaatgatt caagatttac acaaacaaga
    28141 tagataattt agtgttaggt atctggaaaa tgtttgataa tttcttaata tcggtatatt
    28201 aacattaaac agttaataca tagatgtgta gaaatagtta acattttcca gtttttttat
    28261 gaataaattc agttaatacg ctattaaaat atatttttaa aaaagaaggt gacaacatgt
    28321 ataacggtat attaccagta tataaagaac gtggtttaac aagtcatgac gtcgtattca
    28381 aattgcgtaa aattttaaag actaaaaaaa tagggcacac tggtacgctt gatcctgaag
    28441 ttgcaggcgt gttaccggta tgtataggta atgcaacgag agttagtgat tatgttatgg
    28501 atatgggtaa agcttatgag gcaacggtat cgataggcag aagtacaaca actgaagatc
    28561 aaacgggtga tacattggaa atgaagggtg tacattcagc taatttcaac aatgacgata
    28621 ttgaccgatt gttagaaagt tttaaaggta tcattgaaca aattccgcca atgtattcat
    28681 cagtcaaggt gaatggtaaa aaattatatg aatatgcgcg taataatgaa acagttgaaa
    28741 gaccaaagcg taaagttaat attaaagata ttgggcgtat atctgaatta gattttaaag
    28801 aaaatgagtg tcattttaaa atacgtgtca tctgtggcaa aggtacatat attagaacgt
    28861 tagcgactga tatcggtgtg aaattaggct ttccggcaca tatgtcgaaa ttaacgcgaa
    28921 tcgagtctgg tggatttgtg ttgaaagata gccttacatt agaacaaata aaagaacttc
    28981 atgagcagga ttcattgcaa aataaattgt ttcctttaga atatggatta aagg
//
LOCUS       DAMZPP010000014        28561 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMZPP010000014 DAMZPP010000000
VERSION     DAMZPP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 28561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28561
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.014"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..807)
                     /locus_tag="POH73_000476"
     CDS             complement(<1..807)
                     /locus_tag="POH73_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDI6886454.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGL"
     gene            complement(826..1194)
                     /locus_tag="POH73_000477"
     CDS             complement(826..1194)
                     /locus_tag="POH73_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI6886455.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            complement(1438..2676)
                     /locus_tag="POH73_000478"
     CDS             complement(1438..2676)
                     /locus_tag="POH73_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6886456.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGISERTKV
                     IAIQRSKGYDQRPSIPLNEIEQVITRLKEVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFKTKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVMQLKEQGLI"
     gene            complement(2695..3933)
                     /gene="hflX"
                     /locus_tag="POH73_000479"
     CDS             complement(2695..3933)
                     /gene="hflX"
                     /locus_tag="POH73_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDI6886457.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKLQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDEAIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            4045..4521
                     /locus_tag="POH73_000480"
     CDS             4045..4521
                     /locus_tag="POH73_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDI6886458.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            complement(4743..4976)
                     /gene="hfq"
                     /locus_tag="POH73_000481"
     CDS             complement(4743..4976)
                     /gene="hfq"
                     /locus_tag="POH73_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDI6886459.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEAQESTESEE"
     gene            complement(4991..5926)
                     /gene="miaA"
                     /locus_tag="POH73_000482"
     CDS             complement(4991..5926)
                     /gene="miaA"
                     /locus_tag="POH73_000482"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDI6886460.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIGILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSVVKQKLSALEHLDNQQLHEYLAQFDEASAKNI
                     HPNNRQRVLRAIEYYFKTKKLLSNRKKVQQFTENYDTLLIGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            complement(5944..6858)
                     /locus_tag="POH73_000483"
     CDS             complement(5944..6858)
                     /locus_tag="POH73_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6886461.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYPLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPTEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNIKKLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            complement(7008..8681)
                     /locus_tag="POH73_000484"
     CDS             complement(7008..8681)
                     /locus_tag="POH73_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDI6886462.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVNVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDSIIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            complement(8839..10335)
                     /gene="glpK"
                     /locus_tag="POH73_000485"
     CDS             complement(8839..10335)
                     /gene="glpK"
                     /locus_tag="POH73_000485"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDI6886463.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKP
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAYLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            complement(10465..11283)
                     /locus_tag="POH73_000486"
     CDS             complement(10465..11283)
                     /locus_tag="POH73_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDI6886464.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVYAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
     gene            complement(11758..12300)
                     /locus_tag="POH73_000487"
     CDS             complement(11758..12300)
                     /locus_tag="POH73_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDI6886465.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            complement(12306..14315)
                     /gene="mutL"
                     /locus_tag="POH73_000488"
     CDS             complement(12306..14315)
                     /gene="mutL"
                     /locus_tag="POH73_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDI6886466.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENKQEKTFSSEESNSKPFMAENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDDTSVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEIQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            complement(14328..16946)
                     /gene="mutS"
                     /locus_tag="POH73_000489"
     CDS             complement(14328..16946)
                     /gene="mutS"
                     /locus_tag="POH73_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDI6886467.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDHLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDAVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQDTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNMQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQHQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENSNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            complement(17249..17740)
                     /gene="thiW"
                     /locus_tag="POH73_000490"
     CDS             complement(17249..17740)
                     /gene="thiW"
                     /locus_tag="POH73_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HDI6886468.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLLTLKKRGILQR
                     FIK"
     gene            complement(17767..18132)
                     /locus_tag="POH73_000491"
     CDS             complement(17767..18132)
                     /locus_tag="POH73_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HDI6886469.1"
                     /translation="MYNKDDVLKQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNHGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            complement(18133..19677)
                     /gene="miaB"
                     /locus_tag="POH73_000492"
     CDS             complement(18133..19677)
                     /gene="miaB"
                     /locus_tag="POH73_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDI6886470.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLDVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFVKEVE
                     PEMVIQ"
     gene            complement(19811..20104)
                     /locus_tag="POH73_000493"
     CDS             complement(19811..20104)
                     /locus_tag="POH73_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HDI6886471.1"
                     /translation="MKDTLMSVQIIPKTPDNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            complement(20199..21065)
                     /locus_tag="POH73_000494"
     CDS             complement(20199..21065)
                     /locus_tag="POH73_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HDI6886472.1"
                     /translation="MPTFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEQVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            complement(21066..22826)
                     /locus_tag="POH73_000495"
     CDS             complement(21066..22826)
                     /locus_tag="POH73_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HDI6886473.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLSEIDGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSNGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            complement(22966..23763)
                     /locus_tag="POH73_000496"
     CDS             complement(22966..23763)
                     /locus_tag="POH73_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDI6886474.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IKINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            23937..24152
                     /locus_tag="POH73_000497"
     CDS             23937..24152
                     /locus_tag="POH73_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886475.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
     gene            complement(24448..26007)
                     /gene="rny"
                     /locus_tag="POH73_000498"
     CDS             complement(24448..26007)
                     /gene="rny"
                     /locus_tag="POH73_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDI6886476.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(26361..27404)
                     /gene="recA"
                     /locus_tag="POH73_000499"
     CDS             complement(26361..27404)
                     /gene="recA"
                     /locus_tag="POH73_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDI6886477.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            complement(27569..>28561)
                     /locus_tag="POH73_000500"
     CDS             complement(27569..>28561)
                     /locus_tag="POH73_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDI6886478.1"
                     /translation="RHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLK
                     YIESYFEEQGQEMTPNNKQQALVIEGSTVLANHHGMAPGMMVNFENKQIILLPGPPKE
                     MQPMVKNELLSHFINHNRIIHSELLRFAGIGESKVETVLIDLIDKQTNPTIAPLAGSH
                     EVYIRLTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKINEPFVIY
                     DGITNGALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSA
                     IILLEYDGVVHIGYDNNLEFKTEQFKMSESRNLLKNRSQNYALIRLLNWLRTTN"
BASE COUNT         8647 a         5442 c         3890 g        10582 t
ORIGIN      
        1 taagcccatt tcagtatttg gatcgaagaa tgcattttct ttacctttga ataatgaaac
       61 gttaaagtgc ataccgctac cattcacacc aaataatggt ttaggcataa atgttgcgtg
      121 taaattatgt ttacgtgcga ttgttttaac aaccaattta aatgtttgga tattatcaca
      181 tgctgtaaca gcatctgcat atttaaagtc aatttcatgt tgaccagggg caacttcatg
      241 gtgactagct tcaatatcga agcccatatc ctctaattct aaaacaatat cacgacgaca
      301 gttttcacct aaatctgtag gtgctaaatc gaaatatcca ccatcatcat taagttctaa
      361 agttggttcc cctttttcat ccaacttaaa caagaagaat tctggttcag gccctaggtt
      421 aaagtctgtg aagcctaaat cttccatttc ttttaataca cgttttaagt ttgcacgagg
      481 atccccttca aatggtgttc catctgtttt atatacatca caaattaaac gtgcaacttt
      541 accttgtcca gcagtccatg ggaagattac ccaagtatct aaatctggat gtaagtacat
      601 atctgattct tcgatacgta cgaaaccttc gatagaagaa ccgtcaaaca tcatttcgtt
      661 atcaagtact ttttctaatt ggcttacagg cacttcaaca tttttaattg ttcctaaaat
      721 atcagtgaat tgtaatctta aatatcttac attttcctct tctgcaaatt tacgaatgtc
      781 gtctttagtg aaagtacgtt ttggcatttt aaaatcctcc aaattttatt taataaatcg
      841 ggataaatca ccacgattta ttggcaaagt ttctccaata ggcttttgcg tggcatctac
      901 aatcatcttt tttcttatct cttgttcatc tgttgttaaa tgctcttgtg agtcataaat
      961 gatttgttta atccctttga tattaaatcc tttttctaat aatgatttaa tttctagtaa
     1021 tctttccaaa tcattgagtg agaacagacg tttttgacct tctgttcttt caggtttgat
     1081 gagttcatgt gtttcatagt aacgtatttg ccttggcgtt aaatccgtta acttacttac
     1141 tacactcata gagaagacag ccatatttcg tctgattgca tcattcgata tcattgttcc
     1201 tctcctctac ttttgaactt gttaataact tatcataatt tttttaggat ttcaaaaata
     1261 tgtcaggttt tctaacataa agctcaaaac attgatataa ctgcgatttc gacctctaaa
     1321 acgccataaa acgacctaca gctaaaatat tttttcaatt tttaaaaata atctgataaa
     1381 tattcgcaaa aaaatttcac ccaaaacata ttatcattta gggtgaaatt taatctatta
     1441 tataagtcct tgttctttca actgcataac agctcttgtc acagcaattt taacgtgttc
     1501 atatgttaat cctccttgaa catatgcttc ataaggagga cgaataggtc catctgcaga
     1561 taattcaatc gatgaacctt gaataaacgt accagctgcc ataataacat catcttcgta
     1621 accaggcata taactaggtt ctggactaaa atgtgcatta attggggatg cgtgttgaat
     1681 actttgacaa aatgaaatca tttgttcttt cgttttaaat ttaactgttt gaattaaatc
     1741 agttcgtttt tcgtagtact tcggcgttgt gttcatattc attttttcta aaaataaact
     1801 agtaaacaat gcacctttaa gactctgact gacaacgtgt ggtgctaaaa agaaaccttg
     1861 atacatttca agcaatgcat ttaatgatgc acccgcttct ttaccaatac caggtgctgt
     1921 caatctataa ccacatcgtt caattaaatc ttttctacca gcaatgtatc caccaatctt
     1981 agctaaacca ccgccagggt ttttaattaa tgatcctgct attaaatcgg caccacattc
     2041 tataggttca cgtctttcaa caaattcccc ataacagtta tccacaaata ttaaaatatt
     2101 aggatgcact tctttcaacc tagtaatcac ctgttcaatt tcatttagag gaattgaagg
     2161 tctttgatca tagcctttcg aacgttgaat cgctattact ttggtgcgct cagaaatccc
     2221 atctaacaca ctttcaatat cgatcttacc ttctttaagt gcaatatctt tatatgatac
     2281 gccgtgctcc attaaacttt caataccatt tccgtttaca ccaatgactt caagtaaagt
     2341 gtcatatgga cttcccgtta tataaattaa ttcatcacca tgttttaata aactttgtaa
     2401 tgcaatagta atcgcatgcg tacctgaaat aatttgtggg cgaacaattg catcttctgc
     2461 tttaaatgcc tgcgcatata tttcttctaa atgatcacgt ccaaaatcat catacccgta
     2521 tcctgttgat ccttgtagat cactttcggt tgctttgaca tgatgaaatg catttaatac
     2581 tttttcttgg ttaatatatg cagtttcttc aatttcttta aaatatggtg ctaacgttga
     2641 ttcgacgtca gctactatct tacttatatc tttcatttga atttcgtcct tttattattt
     2701 tttaaatcct tttatacgat aaacattttc aatttcatca tatttaagtt cagtcactaa
     2761 tgtatgttgt tttagaaaat ataatcgatc tgcattattc gtcgcaattg cctcatcata
     2821 ataagttaat tgccttttga tttcatcaat aaataaagtc ttaacaagta atttatcacc
     2881 atcattttta gatgaaacaa aaacattagg caaatcactt gctggacgat ttgatgcatg
     2941 atcacataag tcctttttat taaaaataac tatttgagaa atatgactca tatctaattg
     3001 tttgattaaa tcattaactg tgtcatactg cgtacggtat tcaggatggc tactatctac
     3061 gacatgtact aataaatctg cacctttagc ctcttctaaa gttgatttaa aagctgcaat
     3121 taacgtcgta ggtagtttct gtataaaacc aacagtatct gaaataatta aattaaatcc
     3181 atcatttatt tgaatttgtc gtgttttagg atctaacgtt gcaaataatt gatctttttc
     3241 atacgtctct tcatttgcta aaacattaaa ccatgatgat ttaccagcat ttgtataacc
     3301 aactaaagct acttgaaaca cctgattttg atttctttta tttcgatatc tttcgcgatg
     3361 ttcttctacc gttcgcaatt gaagttttat ttcattcata cgagttcgaa tatgtctacg
     3421 atccatctct aacttcgttt caccagggcc tctagttcca ataccgccac ctaaacgaga
     3481 aagactttta ccatggcctt gcaatctagg taataaataa tcaagttgtg ctagctctac
     3541 ttgcaattta ccttctttac ttcttgctct taatgcaaat atttcaagaa tcaactgagt
     3601 tctatcaata atttttacac ctaaagcttc atttagtgat ttggattgtg cagtcgttaa
     3661 ttcatcattt gtgatgacta catcaatatc tttgaactca ataaatgctt gaatttcttc
     3721 aattttacct ttaccaacat aatatttgcg atctacacga tctctgtttt gagtaatttg
     3781 acccaacact tcaagttggc aagtctctga taaagatgat aattcctcca ttgtagactc
     3841 aaaattaaat tgcttatcat cttgagcatg tacaccgact aatacagctt tttctagttt
     3901 atttttagta tcatgaattt gttgctgagc catgttatac atctccttaa taaaatccta
     3961 ctcaaagtat tctatcatag agttttagta aagactatta cacgatttta tggtacattt
     4021 taagtaaatc gaaaggatga ttcaatggag acaatttatg attttgtagt agaaacaaat
     4081 aaaggtgtta cttacaaatt agatgcatat aaaggtgacg ttatgctaat tgtcaataca
     4141 gctagcgaat gtggctttac ttcacaattc gaaggtttgc aatcattata cgagaaatac
     4201 aaagatcaag ggtttgtaat tctaggtttc ccttgtaatc aatttggtgg ccaagaacct
     4261 ggttcaggcg aggaagcagc tcaaaactgt aaattaaact atggtgttac cttccctatg
     4321 catcaaaaaa tagacgtaaa aggggaacat caattacctt tatttagata tttgactgcg
     4381 gcacaacatg gattctttaa tgagaaaatc aaatggaatt tcactaagtt tttagtggat
     4441 cgtgaaggta acgttgttaa acgatttgca cctcaaaaaa aacctgttca aatcgaaaga
     4501 gaaatagaaa agttattata aaatatacta gctcatagca aataaaacgc ttcatataaa
     4561 gtcgtatcat atattgactc gacctttata tgaagcgttt ttattacaaa cattcctcta
     4621 ttgtatcttg tcatttgagc atcatttaat tgatgtcaca tatgacttta tttctatatc
     4681 tatttatcat acttcaaacg ttatgtacac gagtcattaa atcgtgccca tatgattaca
     4741 acttattctt cactttcagt agattcttga gcttcagttt ctactgtata agtgctgatc
     4801 gcatgtttgt aaatcaagtg ttgtttgcct tgagaattta agcttacgac atacttgtcg
     4861 tattcttcaa taacaccttt catttggaaa ccgtttagaa agaatacagt tacttcagtt
     4921 tggtttgctt taaaattctc tagtgctttg tcttggatgt tttcgtttgc aatcatctgt
     4981 cggactcctt ttacttaatc tgggttgtaa tctcatctaa catcatttga agtgacatat
     5041 tttctttatc taaccaatga acactcattt tattcttgaa ccatgtcatt tgtcgttttg
     5101 catattggcg tgaatgttgc tttaaatcat tgacagcatc ttcataaatc atttgtccgt
     5161 taatcacagg tattaattct ttatatccaa tagcttgcat actttggcaa gattcatagc
     5221 cttgttcaac aagttgttgc acttctctaa ataatccgtg atccaacata atatcaacac
     5281 gtttatttat tcttgaatat aatgttttac gcgacatttc aatccctatt aataatgtat
     5341 cataattttc agtaaattgt tgcactttct tacgattact taaaagtttt tttgttttaa
     5401 aatagtattc gatagcgcgc aatactctct gtcgattgtt tggatgaata tttttggcag
     5461 aagcctcgtc aaattgagct aaatattcgt gtagttgctg attatctaaa tgttctaatg
     5521 cagataactt ttgtttaact accgataatt gtgcaggtgt aactgtttca tcttctaatt
     5581 cataattata tattaatgat tgaatatata agcctgttcc acctgctatg attggaactt
     5641 tacctctatt cgttatatca gtaattaaat cttctgctaa tcgcttgaat tcatatgctg
     5701 aaaatgtatc atcaggattc aagataccaa ttaaatgatg tggaatacca tccatttctt
     5761 caggtgttac ttttgcagtt ccaatattca tatgtttgta gacttgcata gagtcaccgc
     5821 ttatgatttc accattgata cgcttcgcga gttcgatgct aagctctgtt ttacctgaag
     5881 cagttggccc cacaattact acaataaaag gcttattttt attcatattt aattccactt
     5941 tctttatttt ttataaaatt gattcaacca tttaaacaag tcttcccaaa ttttgtcatg
     6001 atctttttca aataacactt catgacgttt atgatgataa agattaactt taacattttt
     6061 aatgccggct tttctatata tattcgccaa ttttaaaatc cctttaccat aatcacctaa
     6121 aggatcgtca tatcctgaaa ctaataatat aggcatggca tgatttaact tttttatatt
     6181 ttttaattgt gatgtatgta gcatataata tgctgtttga tataataatt gatttgacac
     6241 attaaatcca ctatatggat ctttaataaa gttgtctact tcagttggat tactagaaat
     6301 ccaatcactt tgtgtacgta atggacgtat ttttttatta aaactatttg atactaactg
     6361 gttaacccat tcaacccgtt tctcggcacc ataaattttt gtaatcagtt gtaacacctt
     6421 aacggttggt aatcctttcc ataatggata cataccagta ccacttaaaa ttagaccatc
     6481 aacatattgc ggatatgttt ctacaaacaa tctagctata acggatccca ttgaatgtcc
     6541 gattataatg tatggtttat caacattgcc tctcactgtt tgcgctactt caaaggcatc
     6601 accgataact cgtttcatat catcgtaatg ccctcttgtt gattcatcaa tattaatacc
     6661 atgtcctcga tgattatgac gtatcacatc gaagccatgc ttagaaagtg catgtgctaa
     6721 tttatcgtaa cgttccatat gttcagccat accatgaaat atatgaataa ttccttttga
     6781 atctacatct gtaaaattca ctttaacttc aatattcgta ttatcttgta cagttaattt
     6841 aaattgtttt tcagtcatca tctcacacct ttaataacaa cgtttttata ttcgctttta
     6901 ttattatcat acatttagtg atgcatagtt tcaattgact aatgtatctc atataaaaaa
     6961 actgatgccc ttaaaacagg acaccagttt atgattgtac aaattaatta ttctttaact
     7021 gctggttgat tatttccatg ttgcgcttct ttaattgctt gctcaacttc ttcagtataa
     7081 gcctcaattt gagctggaga atagtctaac atatcagcca taatatcgat gatactatct
     7141 ttataatcta atacatcttt aatattgaag tacattttac cagaacgacg aactaagaaa
     7201 tcgttaggtt tgtataccat ttcttgttga atactataaa caagttctac ataaatttct
     7261 aatggtaact tgctgtcatg gtattgagat gtttgcgcaa tgttgaacaa ttcatcaaca
     7321 tttgaaccat atttagatgc taaacgtctc gcaacatctt catcaatgcc gaatccttta
     7381 gctacatcta ctttttgctc tacaaacgca tcaaagttct tgctaccacc tacatcgcca
     7441 ccagaaattg ccagaccttt tgtattacat ggactaaatg ttaaaccata gtcttttttc
     7501 aagcgtttag atactaaatc aacaatgtct tgagccatgt gacgatagcc tgttaattta
     7561 ccacctgcaa tagttaataa acctgattta ccttcccaaa tttcatcctt acgagagatt
     7621 tcagaagggt ctttgccctc ttcgtaaatt aatggtctaa ttcctgccca tgttgattca
     7681 atatcttcat ctgtaacatt aacactaggg aacatgtaat taatcgcatc gattaaataa
     7741 tctctgtctt cttgtgtagt taatggtgaa gatttgatat tgtcatagaa tgtatctgta
     7801 gtacctacat acgcttttcc ttcacgcggg attgcaaaaa tcattcttcc atctttttca
     7861 gtatcaaagt atactgcttg acctaatggg aattttgatt gatcaataac aacatgtaca
     7921 cctttagtta aacgtaattt tttattattg cgtgcataat caccacttct aacatcatca
     7981 acccatggac ctgctgcatt aaccactttt ttagccttaa ttgtataatt ttcattagtt
     8041 aatttatcta taacattaac accatttact tgttgatttt tatcataagt gaagtgttct
     8101 gacttagtat agttgataat ttctgcgcct ttttcggcag cacgcttcat aacttcaata
     8161 gttaaacgcg catcgtcagt acgatattca acatagtaac cgccgccttt tagaccttct
     8221 tttttaacta atggttcttt agctaaagtt tcttttttag ataacatttt tttacgttca
     8281 gacttcttaa cacctgctaa acgatcatac attcctaaac caattgatgt tgagaattta
     8341 ccaaatgttc cacctttatg cattggtaaa agcatccact ctggagtcgt aacatgaggc
     8401 ccattttcat aaacaatcgc acgttcttta ccagtttcgg caactactcc tatttggaat
     8461 tgttttaagt aacgtaaacc accatggact aattttgtag atcttgagct tgttccttgt
     8521 gcaaagtctt gcatttcaac taatgcaact ttcattcctc tttcactcgc gtctagtgca
     8581 atacctgcac ctgtaatacc gccaccaata attactaaat catattcatc atttcttaaa
     8641 ttctttttaa tatgttctct cttaaaagta gacaatgcca ttccaaaacg cctcctaaaa
     8701 aatgtaaaaa cgagagactt gtcccacata aacaatcctt caattgtaca tgttcgaaca
     8761 aatctctcgt ttctcgattc aaatattaac ttaaattgat tgtatcacaa tgtttataca
     8821 aaagtctaat ctacaagttt attctgtttt aaaaacttgt gttgcttcaa cagctttttt
     8881 ccaacctcta tataattttt ctctttcgcc ttcatccatt ttcggatcga atttttcttc
     8941 taatttccag tttttagcga tatcatcttt actctcccag aatccaactg ctaaacctgc
     9001 taaatatgca gcacctaaag ctgtagtttc ttgaatttca ggtctttcaa cagaagtatt
     9061 aacaatgtct gcttggaact gcataataaa gttattctta actgcaccac catcgacacg
     9121 taaactttgg acatcgatac cagagtcttt tgacattgct tccataacgt cacgagtttg
     9181 gtaacataat gattctaatg ttgcacggat aaagtgctct ttttcagttc cacgtgttaa
     9241 accgaaaatc gcaccacgtg cttcagaatc ccaatatggt gttcctaaac ctacaaaagc
     9301 tggaacaaca taaacaccct cagtagagtc aactcgtgtc gcataacttt ctgattgtgg
     9361 tgctgaatta atcattctta atccatcacg taaccattgg attgctgaac ccgaaacaaa
     9421 gatggaacct tcaagcgcat aatttacttt tccatcaata ccataagcaa ttgttgttaa
     9481 taaaccactt tcagatttaa ccgctttgtc acctgtattc attaacatga agccaccagt
     9541 tccatatgtg tttttcacgt caccacgttc gaagcaagct tgtccaaata atgctgcttg
     9601 ttgatcacca gctacaccag caattggtac ttcttgacca tagaagtggt aatcaattgt
     9661 cttaccatat acttcacttg aaggtttaac ttctggcaac atatttttag gtactgtaag
     9721 tagttctaat aactcatcgt cccattctaa atcatggata ttaaacatta atgtacgact
     9781 cgcatttgaa taatcagtaa tatgcgcggc ttttcctgat aatttccata ctaaccaagt
     9841 atcaatcgtt ccaaataata gatcgccatt ttctgctttt tctcgtgcac cttcaacatt
     9901 gtctagaatc catttaactt ttgtacctgc aaaatacgga tctaaaagta atcctgtctt
     9961 atctctaaat gtttgttcat atccttgttg ttttaattct gaacaaattg attgtgtttg
    10021 acgtgattgc caaacaattg cgtgataaat tgggcggcca gtatgtttgt cccaaacaac
    10081 cgttgtttca cgttggtttg taataccgat acctgcaatt tgatcagctc taacatcatt
    10141 ttcattaatt acttccgtca ttacagctaa cacagatgtc caaatttcat ttgcatcatg
    10201 ttcaacccaa cctgattgtg gaaaatattg cttaaactca cgttgtgcta cccctgcaat
    10261 ttcccctttt tgattgaata aaatcgctct tgagcttgtt gttccttggt ctatagataa
    10321 aatatatttt tccattagta gtatctcctt tattaaaaaa ataatcgcta acccattgta
    10381 caaaatccgt taaattttgg cgaaaaaata gcataatcca tgaatcaaac atgttaaatt
    10441 atgctattta catttctttt tattttagta aattgattcg atatcacctt tttttgatga
    10501 tttatttaaa atgtaaccta aaatcaaagt aataataact acaacaattg caattgcaca
    10561 accaatatta aatgtatgtt tataaaatac agcgtatact accgcaccta ataaaccacc
    10621 ggcaattggt cctaagatag gaacgattgc atatgaccaa tttgaaccac ctttgccagc
    10681 tattggtaaa atcgcatgtg caattctcgg acctaaatca cgtgctgggt tgattgcata
    10741 accagtagca ccgcctaaac ttaatccgat tgcaacaatt aatgctccga caattaaagg
    10801 atttaaacca tcagcaattt tgtttacacc gataaataaa atacctaaag ttaatgccat
    10861 tgttccgata atttcactta aaaagttggc aaagtaattc ttaatagccg gtgctgtaga
    10921 gaaaacacct aatttcgcgc cagcttcttc tgtcgctttc caatgtggca agtacattaa
    10981 ccatacaatt gttgctccga caattgcacc taacatttga gcaacaatat aaccaggaac
    11041 taatgaccaa tcaaaacttc cgtctaatgc aagagctaaa gacaccgctg ggtttaaatg
    11101 tgcacctgag aattgaccaa cagcatacac acccattgta accgctaatc cccatccagc
    11161 tgtgatgaca atccaatcag caccattcgc agcacttctc tttaaattga cattggcaca
    11221 aacgccacca ccaaaaagga ttaagattgc agttcctagg aattctgcta aatatacatt
    11281 catattaaat gtcctcccaa aaagacaaag agattcctac actagcagtt tggctcgtag
    11341 aaatctcccg tctctattct atgtattaac ttttattata tttgttataa tactattgag
    11401 atgaaagcgt tgtcaatggt ttttgtaaaa attttgtcaa atttaatttt tcaagtcttt
    11461 ataaaagtga tttaatttga aaactagaaa tatcaatcct aactaaatta tttatgacgc
    11521 cattttgttt aaatgacatt cttcagccat attttttatg actagttgca ttttttacaa
    11581 ataaacacct ctataacttt aatgatttta atcgttttag aggtgctaat tattttattt
    11641 aattattctt tgtatgatac cttaaattcc ttattacata tcccatatga aaacggcttt
    11701 tttaatatgt tatatgacta aatctagtga aaaatctaat ttttgcagac ttatgattca
    11761 ccagagttta tcataactag ttgttacata tttcgctcca tttttaacag cttcattaac
    11821 ttcatctatt gtattaatta ggccacctgc aatgacttgt gtgtttgttt ctttctgaat
    11881 atgatgaatc gctttactcg caacacccgg aagtacttca acaaaatcag gttcaacttt
    11941 ttttatcaaa tctatactgc gtttcaatgc ttgactatca ataataaaca ctctaaaaat
    12001 cgttaaagta tttaatgatt tcgctttttt tattacttta gatttagtcg atacaatacc
    12061 ttttggcttg tattgctgaa taataaattc acttgcaaat tcatcgtgac ttaaaccttt
    12121 tatcaaatct atatgaataa aacactctat atgattttgc ttcagcaatt ccataatact
    12181 ttttatatgt cctatatgca tatctagaag cacacacatt ttataatctg ttttaatcag
    12241 tttctctaaa tctttaatgt ttcttatggc aggcaatatg ttgttattca cttgattcat
    12301 cctctctaca tcacacgctt aaataatttt tctaattcgt attttgaaaa attaatgata
    12361 attggacgac catgtggaca tgtaaatgga tcttccgctt ctcttaattg atcaattaaa
    12421 tcagacattt catgtttttg taaataatga ttcgctttaa tagatttttt acatgacatc
    12481 atgattgcta catcttcacg taattttttg atatctactt ttttctcttc caaaattagc
    12541 tcaatcatat ctttaataat ttcttctact tcatctttag ggaaccaaac tggatagcta
    12601 cttacaatat aatcatgacc accaaaatgt tctaacatga tacctacttg ttgaagctca
    12661 tttttatatt gatcaatgac taattgttca tcttttgaaa aatgaaatgt taacgggatt
    12721 aataaatctt gtatttcatt ggtaacttca cctattttat ctcgaaaata ttcatatttt
    12781 attctttctt gagctgcatg ctggtcaatc atgtacatgc caaattcatt ttgagcaata
    12841 atatacgttc catgtacttg gccaacaatt tccatatatg gaattcttcg tttagggtct
    12901 ttactttttg ttcctttaat atcgtttaca tcataataat catcagacgc tttattctca
    12961 taattttgca cagatgtatc gtcatttgaa tcaaatgttt gatccatttc ttgtaaaatt
    13021 tcttgttgtt ttttgaagta gtcttcatct ttttcacgtg tattattata accagaagat
    13081 tcatcatcag ctattaaact ttcagatgtt ttcgttacga atggattata tgaatcttct
    13141 tttataacta tctcatcgtt ttgattttct gccataaatg gcttactgtt actttcttca
    13201 gatgaaaacg tcttctcttg tttattctct gtgttttgtc tttgttcaaa ttcgattttt
    13261 tgttgttcga atgaatgtaa cactttattt tttttcggca cataatccaa gttattttta
    13321 ggaattaata tacggtcttt aaatgcttct tgtattttgc tcactatcaa ttgatatagt
    13381 tgctcttctt ttgataaacg cacttctagt tttgttggat gaacatttac gtctactaag
    13441 attggatcca tttcaatatt aatataacaa atcgggaacc tacctattgt taagagtgta
    13501 tgatagcctt ctaaaatcgc tttatttaac ataaagtttt taatgtatcg tccattaata
    13561 aaaatagaaa tatagtgctt attacttcta gaatgttcag gctttgcaac aaaaccttca
    13621 atgtgataat cacttgtatc tccagatata tgtactaaat ctcgtgcaac tttcatccca
    13681 taaatctctg ccatcacttc attagttctt cctgaaccat ttgtacttaa cattgttttg
    13741 ccatctgaaa tgagtgctat tcgaatgtcc ggatgactca ttgccattct gttgacaata
    13801 tctgttattt tacctagttc agtgtataaa ctttttatat attttaaacg tgctggtgta
    13861 ttataaaata atgattctac aagtatatct gttccttttt tcgcttttgc aggcttatga
    13921 tttaatattt caccattttc tacatatatt tcatttccat tagcattatc cgtgcaagtc
    13981 ttcaatgtta ctttagcaac tgatgaaata ctggctagtg cttcaccacg gaatcctaac
    14041 gtcctaatat gaaataaatc ttcatcttga tctaacttac tagtcgcatg tctatgaaat
    14101 actaatccta agtcttccgc ttcaattccg cttccattat cgactacgcg aatagattgg
    14161 acgccagatt cctctacttc aatgcttatt tctgtagcgc ctgcatctat agcattttcc
    14221 aacagttctt tcacaacaga actcggtctt tcaactactt cacctgctgc gattttattt
    14281 gctaatgagg tttggagttc tttaattttc cccattttgc aacacctcta ttttaattga
    14341 ttttgtaatt cacttaactt caccaatgcc tcaattggtg tcatattaga taaattcaaa
    14401 tttttaattt gtagttcaat ctcgctttct tgatcatttt caaacaaatc aaatgatgct
    14461 tgttcaaagt ctttttgaga taaagtatca gttgtttctt caacacttaa gtttgaattt
    14521 tcttgattaa tttcaggttc attttcgacc atttttaaat ttgatatcga tgatttttta
    14581 ccagcagacg cttcaaactc gcttaggatc acttgtgctc tgctaataac tttttcaggt
    14641 aaatcagcta atttcgcaac ttgaatacca taactatcgt caactgcacc atctttgact
    14701 ttatgcaaga atataagttc acctttatat tcattagcag cgacgtgaac attttttaga
    14761 cttggtaatg cttgatctaa tgttgtcaat tcatgataat gtgttgaaaa taacgtttta
    14821 gcatgtgatg tttcagctac atactctatc attgcctgcg ctaaagctaa accgtcatac
    14881 gttgaagtac cacgtccaat ttcatcgaaa ataatcaaac tatcctctgt tgcataagtt
    14941 aatgcctttt gtgcttctag catttctacc ataaacgtac tcttacctga aaccaaatca
    15001 tctgccgcac ctattctagt gaatatttga tcaaatatag gtaacacagc ctctttacaa
    15061 gggacataag ctcccatttg ggccattata ctaattatgg caacttgtct catatacgtc
    15121 gatttaccag acatattcgg acctgtaatt aaatatataa atgtttcgtt atctaatcga
    15181 caattattag gcacatagtc attataatcc attactcttt ccactactgg gtgcctagat
    15241 tccactaatt ctaatgtttt attttcacta aatgaaggcc tagtataatt atatttttga
    15301 gcaatttctg caaagctctg taaacaatct aactctgaaa taattttagc ttgatgttgt
    15361 aaacgttcag tatatttttt aacttcttca cgtagctgaa caaataattg atattctaat
    15421 tcaatggctt tgtcttccgc acctaaaatg atatcttctt tttctttaag ttcatcagtt
    15481 ataaatcgtt ccgcattcga taacgtttgc ttcctcatat aaccaaattc acttggttca
    15541 aaattttgca agttggcacg tgttatttct ataaaataac caaacacttt attaaagctt
    15601 attttcaatg attttattcc tgtacgttgt ctttcttttg cttgtagttc tgctaaccat
    15661 gtttttccgt tttttgaagc ttcaagatat tcatctaatt gcatattaaa accaactttg
    15721 aatagtccac catctttaac tgaaattggt ggttcttcta ctaaactctg ttctaatata
    15781 tcaagtaaat catcaagtgg ttctagttga ttaacttgta caagagtatc ctgattcata
    15841 gaatttagta atgctttaat attcggtatt tcagaaatgg aatgtttaag ttgaattaaa
    15901 tctctcgcat taacatttcc gtaactaaca cgcccaacaa gacgttcaat atcatacact
    15961 tgattaagat atgttcttaa ggtgtctctt tctatgaaat gagcactaaa ttcatcaacg
    16021 atatctaatc gtgcttcaat ttgttcttta cttattagtg gtctatctat ccattgtttt
    16081 aatcggcgtg ctcccattgg tgttttcgtt tcgtccatca gccaaagtag cgttcctttt
    16141 ttggatttta atcgaatgct ttccgttaac tcaaggtttc tcttagcata aaaatccatt
    16201 ttcatataat ctatagctgc atattgaaca gcatcctcaa tatgcgataa atcacgtttt
    16261 tgtgtatgat gaatataatc tagcaataat tgtgtcgctt gatacattaa tttatgttca
    16321 gtttgattca cactgtagat ttcagatgat aacgtttccc tgactgttat tgtttctgtc
    16381 accatattaa tttgtctttt taaatgatcg gaaatattgt cattgataac aacttcgtta
    16441 gggtttatcg tcgtaatttc atttaataaa gtcgcttcat cattaaaatg tgtaaccttt
    16501 aattcaccag tagaaacatc acagtaacta agcgcaattt caggttgatt cataacaaaa
    16561 cttaaaatat agttattttg tttatcatct acaccacctt gctccatcac agttcctgga
    16621 gtcacaattc ttactacctc acgtctaacc atacctttcg tttgtttcgg atcttccatc
    16681 tgttcacaaa tagctacttt atatccatta ttaacaagtg tatctatata actatctgca
    16741 gaatgatacg gaacaccaca catcggaatt ggattttctt ttttagcatc tcttttagtt
    16801 aaagtaattt caagtacacg tgatgcctcc ttggcatctt cataaaacat ttcatagaaa
    16861 tcacctagtc taaaaaataa taagcaatct tggtattctg attttatttt taaatattgc
    16921 tgcatcattg gtgtaacatt agacatatta tttcttcaca acccttgtct ctttttaaaa
    16981 tttgtcttta caatatattc gtttgtaagc tttctaatta ttaattattt aacttataca
    17041 ttttaacata cttactttta caaacctatt cataccatat aataacgaag catcttaaat
    17101 gtataagaaa acgcctcaaa cctaataaaa tgtgttaata gcatgtttag aattatatta
    17161 aaattctaac attcaagaca tttaattaag taagggcgtt caatattaaa atgaacaatg
    17221 actctgtttg aaatcatata tcataaaatt attttataaa cctttgaaga ataccacgtt
    17281 tttttagagt aagtaataag aaataactta taatagatcc gatagcactt gagactatga
    17341 acgtaatcat taacggttta atgaagaagt cttgaagccc aaggaaatat gctaatggta
    17401 tacaaattaa acttccgatg acaccagttc caagtacttc accgaccgcg gccatgaata
    17461 tatgtttacg atatatgtaa aacatactag ccaataaaac tccaatcata ctacccggaa
    17521 atgcaaaagc tgtaccagtt ccaaaaagaa ctcttaaaat tgatgatata agcgcttgag
    17581 ctaatccata ccaaggacct actatgaccg cacttaatac atttacaaaa tgctgtactg
    17641 gtgctgcctt aactggtcct agaggaatga tgataatact gcttaataca acatttattg
    17701 caattaaaag tgcagttata gccagttttc ttgatttcat atgattgttc tcctttttgt
    17761 ttgtaattaa tcactatgtt tggctttatt atggtcattt aaacgtgttt ccattgttga
    17821 tacaaacatt ttcaataatt gattcgcttc atattgtgaa gtttgaaact gttcaactat
    17881 gggcaatgta tttatttctg cttctatact ttgaatggta tgttccgact gttctagcgc
    17941 attttgtttc ccgtgatttt gaaagtttac tgcttgtttt tgatgctttt ttaacatatc
    18001 cattttagtc tttatcgttt ggttctgatg aatctgtgct tcaatttgtt gatatgtttt
    18061 gatagtatcc aaatttttaa ttttatttgc aatattatcc gcttgtttca acacgtcatc
    18121 tttattatac atttattgaa tcaccatttc cggctctact tcctttacaa agctgccatt
    18181 taatgaatac tgtttagctt catctattcg tacttccact agtttaccaa tcatttcttt
    18241 aggcgctttg aaattaacta acttattttt atcagtgtag ccagcaagaa cctgatcatc
    18301 ttttttacta ctaccttcac aaagtactgt tacagtttgt ccttcgtact tactcatagc
    18361 tatttgtgaa taatgaccaa cttttttatt caaacgttgc aatcgttcct ttttgacatc
    18421 taaaggtaca ttatctttca ttttagcagc aggcgtacca tcacgttgtg aatacaaata
    18481 cgtatatgca tgttcaaaac caacttcatc atacaaagtt aaagtttctt caaattgttc
    18541 ctctgattca tttggatacc ctacaataat atctgtagtt aatgctacat ttggaattct
    18601 atctttgatt cgttttacta aatccaaata actttctcgt gtatattttc tacccattat
    18661 ttttaatact gcattatttc cagattgaac tggcaagtgg atatgaggaa cgatattacc
    18721 accctctgaa ataacatcaa tcatgtgatc tgtgaagtcc caaggatgac ttgttgtgaa
    18781 acgaactctt ggaatcgcta ttttagaaat tgcttgtaaa aggtctccta agtcatattc
    18841 tatatcctgt aaatctttac cataagaatt tacattttga cctaaaagcg ttatttcttt
    18901 gtaaccttca cgagcaagtt cacgtacttc atctataatg tcttcaggtc tacggcttcg
    18961 ttctttgcct cttgtaaatg gaacaataca atatgtacaa aacttatcac aaccatacat
    19021 aatattgacc catgctttaa tgttgccttc acggactttt ggaagatttt caataacgtc
    19081 tccttcttta gaccatactt caacaaccat tgctttagat aagtatgctt cttctaaaat
    19141 ttctggtaaa tgatgaatat tgtgtgtacc aaatatcata tctacatttt gatacgattt
    19201 taaaatttta ttcactactg actcttcttg tgacatacaa ccacaaactc cgattaaaat
    19261 atcaggtcgt tctttcttca aatgcttcaa attacctatt tcactaaaaa ctttgttctc
    19321 ggcattttct ctaatcgcac atgtattaat taaaataaca tctgcagtgt taatatcagt
    19381 cgttgcttga tagcctaatg cttcaagtat accagcaatg acctcagtgt catgtgcatt
    19441 catttgacat ccatatgttt taattaaaaa tgtacgctcg ttccccatac cgcgatattt
    19501 ttcatcaatt tggaaatctc tattataacg aacttcttgt ttacctcttt ttttcgcttc
    19561 ttttaaatta ggcggttgat aaacatgttc aaaatattta ctataatctt tttctgcttt
    19621 cttatctctc tcagctaaaa catctacaga acttgctttt ctttgttctt cgttcacttt
    19681 acaaaatcct ttctatttgc ccgtatcatt taataattat attaaattta actataaagt
    19741 gcaaaaacag ttaaagttat acgtcatctc taacaagcca attcaaatcc agtttacttc
    19801 acttttaatg ttattcatca tatttttcag ttaaagtttc aatagtaatg ccgtctggca
    19861 catgataaaa cttaacttgg ctaataatac ttggacattc aagttccacc attcgttcat
    19921 ttaacgattg tattaaaatt aaacattcat tcatatttcc ttgtactgtc gtttctaacg
    19981 gacctactct aaaatgcaaa ccagattcgt caattatttt aatcgcctcg tctacgtaag
    20041 gtataacatt gtcattgtct ggtgttttag gaattatttg tacactcatt aatgtgtctt
    20101 tcatttttaa aaactccttt atattttgta atagtaaaaa aactgagtag cataaatgga
    20161 tctgttataa atacatttat acgactcagt tttaattatt aaataaattg ttcagttaat
    20221 gcattgaacg tgtcttcagt aattttgata tctcttttag ctagtggtgt atcatctaac
    20281 tctttgattt gagattcata tgatggggtt tctttatctt gataaacaat accagttact
    20341 aaagattcat gttcaataac agttttagtc gctaattgtt tatctgtaga atcatagttt
    20401 tcaatgtcat caacacttgt taaatgttct ttaaaccaat cgtatgtgtt aattttatta
    20461 taagtcacac atggtgaaaa gacattaaca aatgaaaacc catcgtgatt aattgcatct
    20521 tcaattagtt ttgttaatcc tttaatatca cttgaaaaac cttgggctac aaatgtggca
    20581 ccagatgata atgctaattc taaaggcgca acattttttt ctatattacc ttttggcgtt
    20641 gttttagtaa caaatcctac tgctgatgac ggcgatgttt gtccctttgt caaaccataa
    20701 atttgattat ccatgactat atacgtcatg ttcatatttc ttcttaaagc atggattgta
    20761 tgccccatac caatagcata accatcacca tctcctccag atgcaataac agttaaatct
    20821 ttattcgcca tttttacacc ttgagctaaa ggtaatgcac gtccgtgaat agaatgaacg
    20881 ccataagaat taatatatcc tgaaagacgg ccagaacatc ctataccggt gatgatagct
    20941 acttgttcag gttctaaccc tatatttgcg gctgcttttt gaattgcagc ttgtactgag
    21001 aaatcgccac atccagggca ccagttaggc ttaacattat ttctaaaatc tttaaatgtc
    21061 ggcatctata ccatctcctt tatttcagta gcaattattt tacctttttc ttcgatttca
    21121 tgtggtagga aaggtgtccc atcatacttt gtataatttt caatcttatc atgaatattg
    21181 acattcattt ttataatact agccaattgt ccttgataat tgtgctccac tacaacgact
    21241 ttcttcgctt tattaactgc atcttgaata acgcttgttg ggaatggatg caattgtcta
    21301 atttgtatag tgttaacttt tatgccttgt tgattcaaac gattactacc ttcttgaatt
    21361 gcaccttttg tagaaataaa accgatataa agaatatctg catcttcatg ttgtaagtta
    21421 gcttctactg gcgattcaat tagtaactgc tcaattttac gcattcgttt ttccatttgt
    21481 tgttgtctat ttgacgcaga ttcgctaggt ttaccttctt cattgtgttc cacaccagtt
    21541 atatgatgaa tacctccttt aacacccggg ataggtctag gagaaacacc attggatgtt
    21601 aacgcataac gcttgaaata acctttatca tcttcttcac gttcaatatc agattgaatg
    21661 atttcaccac gtttaatttc aatgcgatta taatctaatt tttcaacagt ttgttttcct
    21721 aaagataatt gtaaatcact tagaactata acagggcatt gatattgttc tgctaaatta
    21781 aaggcttcca tagttaaata aaatgcatct tctgcatcag taggtgctac aactattttt
    21841 ggaatatcac catgtgtacc ataaatcatt tgcattaaat ctgactgttc ttgtttcgta
    21901 ggtaatccag tagaaggtcc acctcgttgg gtattaatta tgactaatgg cgtttcagtc
    21961 ataccagata atccaattgc ttccatcatt aaagataaac ctggaccagc actagccgta
    22021 aacgctctga ctccaccata atttgcacca atagccatag ttacagcagc aatttcatct
    22081 tctgtttgaa taaccgctcc gtttacttta gatatattgg caatcatata ttccataact
    22141 tcagacgcag gtgtaatagg atatgccgcc ataaattgtg aacctgcagc aattgcacct
    22201 aatccaatgg catcgttacc aatcatatat agatgtggta gtgcatctgt tgactctaat
    22261 tcaaagtccc cgtcgatttc agataagcga gattgcatta attgataacc ttcgtttaat
    22321 gcttggatat tgacttcaac taccttgtca ccttttttag aaaacatatt agtaataagt
    22381 tcttcaaatg tatttgtatt caaattcatc aatgcgctag tagcacctat tgcaaccatg
    22441 tttttcatta atgctgtacc taattcttta gcggttgctg taaaaggtaa ttcaataagc
    22501 tgtgcatgac atccttctgg tttcacaggt ttagccttgg catcagctaa aatgatactg
    22561 tcttctctca tttcatgatg gttaacatca attgtttctt ggtcaaatgc aatcaaaata
    22621 tctaaatcat cactaattgc atgaacaggc gtcgtagaaa ctctaatttt attattcgta
    22681 tgtccacctt tgatacgact tgaaaaatgt ctatatccat ataaataata tccttttcta
    22741 ttcatagccg ttgcgaagat ttccccagtt gattcaatac cttcgccttg ttgaccgcca
    22801 actttccacg ataattgtgg tttcatagtt atatgcctcc tatggatata tttcatatta
    22861 aatgataaca aaaagaacca ccgatttact atgtaaacag tggtactata ttaaattcat
    22921 actaatggac gatagggtcc aattagaatg tttacttacg taattttaaa atgttgaaaa
    22981 tggatggtca tcatttatca atatacgttc gatgtgcttt gttttacctt ctttgtccaa
    23041 atcaataaca acaccagata atacacttct accttcattt ggaacaacat gtctttgtgg
    23101 caaactagtg ataaaacgct caattacctc tgttttattt attcctaaaa tgccgtcata
    23161 aaagcctgtc ataccaacat ccgttatata ccctgtaccc tttggtaaaa tacgttcatc
    23221 tgctgtttga atgtgtgtat gcgttccaac aacagcgcta gctctaccat ctaaatgcca
    23281 tcccattgca tacttttcag aagttgtttc tgcatgaaaa tcaacaaata taaacggagt
    23341 ttgttcttgt gcttccttga ctaattgatc tgccttttta aaaggatcat caatatctgg
    23401 cataaacgct cttccttgca gattaataac tgcaagttta atatcattaa tttttataaa
    23461 tctcatacca attcccggcg cttcatccgg aaaattagct ggtcttacta gtcgttttgc
    23521 ttcatctata aaatcataaa tttcacgttg accatatgtg tgattaccca tagtcatgaa
    23581 atctacacca tttcttagta attgtttata tattttttca gtcaaacctt taccatgcgc
    23641 tgcattttca gcatttacaa ttgtaactgt tggtttatac ttttgcttca gttgaggtat
    23701 gtacgtttca attgcgtctc gtccaatttt acctacgata tcccctataa acattattct
    23761 catgttttgt tcatcctctc ataaatacta gtttaattca tacgttctaa tatttaaagt
    23821 aaatattaaa ttctttgaat tatttttctt caattataga gagacattac tttgtctgaa
    23881 aacttcaata tgttagtatt aagacatcaa aataaatagg agttgtaaat aaatacatgt
    23941 ctattaatat tgatccagaa aaatttgcag aattagtggt tatgtctaac ccatcaaaat
    24001 tcgaagatgc agaggatata gctaaagaaa gtttaaagct ttacattaat gcatatcgtt
    24061 tagcagagag atattcaaca attgcaacaa attgttatga tactgcagaa gttataaaag
    24121 aattaaagaa aacagattta caattaaaat aactcaactt agaaataatt tctaattaat
    24181 attacagaag agaagatgtg ttacatcttc tcttcttttt tttagtttga aaaagcaatc
    24241 acctttatag gtttacataa tataaatata gtaattatga acctaaaaca tttttattaa
    24301 aagcaataac ttcttattta atataaggtc catcttactt tcaaataact actgtctaat
    24361 atagttatta ccaataaagt actgtctaac tagattgaaa ctacaactta aaaaagctcc
    24421 ctcactaatt tgtgagggag acaaaaatta tttcgcatat tctactgctc tagtctctcg
    24481 aacaactgtc accttgatat gaccaggata ttgtaattca tcttcaatct gatttttaat
    24541 atctctagcc aatcgataag attttaaatc atcaatttct tcaggagata caatcactcg
    24601 gatttctcta cctgcctgaa tcgcaaatgc tttttctaca ccatcataac tttctgataa
    24661 cgtttctaaa cgttctaatc gacgaatata attctctaat gtttcttttc ttgcacctgg
    24721 acgagccgca gacaatgcat ctgcagcagc aacaaggata gatataatag atgtaggttc
    24781 aacatcacca tggtgagaat ggattgcatt aataactgtt tcattttcac catatttttt
    24841 cgctaattct acaccgattt caacatgact accttctact tcatgatcaa ttgctttacc
    24901 aacatcatgt aaaagtccag ctcgtttcgc taatgtctca tcttcgccta gctcagcagc
    24961 taacatacta gcaagatgcg caacttcaat tgaatgttta agtacatttt gaccgtaact
    25021 tgtacgatag tttaaacgcc ctacaatttt tactaagtca ggatgcatat tatgtgcgtt
    25081 cacttcaaat gtagcttgtt cacctgcttc tcttataata tcgtctactt cttttctagc
    25141 tttttcgacc atatcttcaa ttctacctgg atgaatacgt ccatcagata ctaagttaac
    25201 aagtgctgtt ctagcaattt ctcttcttat tggatcaaaa ccagataata taactgcttc
    25261 tggtgtgtca tcaataatta aatcaatgcc agttaaagtt tcaagtgtac ggatgtttcg
    25321 tccttcacgt ccaatgattc gacctttcat ctcatcatta ggtaagttaa ctactgatac
    25381 cgttgattca cttgtgtgat ctgctgctaa tctttgtact gctgtagcta ataattcttt
    25441 tgctgtttta tcaacttttt ctttagcttc tttttctttt tctttaacaa gtactgcaat
    25501 atcttgtgac agttcttcct ctactctttg aagttgctca ttaatagctt cttcttgagt
    25561 gagaccggag atgcgttcta attcttgttc atgcttcatt attaacgttt gaacactact
    25621 ctcttttgca tctacttgtt gttgtttttc ttcaattttt gattcttttt gctctaaaat
    25681 ctcatctttt ttatctaata gatcagattt acgctctaag ttttcttctt tttgaagaag
    25741 tcgggtttct tgtctttgaa gttcgctacg tctttctcgt agttctgctt cagtttgttc
    25801 tcttaggatt tggttttctt cttttgcctc aagtaatttc tcttttttga tattgtcagc
    25861 ttctttatgt gcttgattta caatatcttc ggcagtttgt ctagcttgtg attgcttttg
    25921 aagcaacaaa tttcgggcaa caacataccc tccaacaact cctagaatga tccccagcaa
    25981 aatgagtagg aggcttaata aattcacaca aacacctcct tttctagggt ttgctcttta
    26041 tatgaaattc aatatgactt tatgaaatta tatataaatc atacaaatta attgtacggt
    26101 ttataggaga taagtgtcaa gttatcaatt tgcacttttg cactatattg aatgctattt
    26161 ctactatttt ctaaagtttt gaagctcgat tataaaacta ttgataatgt aaaaataaat
    26221 aaaataaaaa accttatagt ctcgatttga agtgcatccc ataaagttag atattattcc
    26281 agctttatga gatgaatatc actttcaatc aatcctataa ggatatttgc taagtttaac
    26341 tatagatata aatttgtgta ctattcttcg tcaaataatg actttggtgc atcttccgtt
    26401 tcttcaacat caccgtcaga tatacctaat ttttctctca atttacgatc aatttcttct
    26461 ttaatttgtg gattttcttt caagtacatt ttaacatttt ccttaccttg acccattcgt
    26521 tcgccattgt atgaatacca tgctcctgat ttatcaacga tgtcgttttc aacacctaaa
    26581 tcaataagtt caccctcttt agaaatacct tgtccataca taatatcaac ttcagctact
    26641 ctaaatggtg gtgccacttt atttttaacg actttaattt tagttctatt acctacaatt
    26701 tcttgtcctt gtttaagctg ttctgcacga cgtacttcta gtcttactga actatagaat
    26761 tttaatgcac gtccacctgg tgtagtctct ggattaccga acataacacc aactttttca
    26821 cgaatttggt tgatgaaaat agcagttgta tttgatttag aaatagcacc tgaaagttta
    26881 cgtaacgctt gtgacattaa acgagcttgt aaaccaacgt gagtgtctcc catttctcct
    26941 tcaatttcag ctttaggtgt taaagcagca actgagtcta caactacaat atcaactgca
    27001 ccacttctaa caaatgcttc agcgatttca agaccttgtt caccatgatc cggttgcgat
    27061 aaatataaat tatcgatatc tacgcctaat gcttgagcat attctggatc taaagcatgt
    27121 tcagcatcga taaatgctgc caccccgcca ttactttgta cttcagcaat agcgtgaagc
    27181 gctactgttg tcttaccaga actttcagga ccataaattt caataattcg tcctttagga
    27241 tagccaccta cacctagcgc attatctaat gtaactgaac cagttgatgt agttgaaact
    27301 cggcgaccta tattgtcacc caacttcatt acggcacctt taccgaaaga tttctccata
    27361 tttttaatta ctgtatctaa agctttttga cgatcgttat ccaaagcgag acctcctaat
    27421 tgaaattgct atcttttaat tatcttacca atactataca aggattcaaa ataaaataca
    27481 agtgttttgc gaatatttgt tcgtgtaaaa tcttgaaatc aaaacacaaa aacgaacaaa
    27541 cgtgcttata tttttatcga taatacaatt aatttgttgt tctaagccaa tttaataacc
    27601 ttataagcgc ataattttga cttctgttct ttaataaatt tctagattca gacattttaa
    27661 attgctcagt tttaaattct aagttattat catagcctat atggactaca ccatcatact
    27721 ctaatagaat aattgctgat gacacattaa ataatttatt aacaaattgc actgcatctt
    27781 ttaattgctg ctcaataggt ttattaatat caacaaagtt ttcattgtga ttaatcatac
    27841 cctttagaac atcgtttaaa tccacttctt tcaatcgatg atataaagca ccattagtaa
    27901 taccatcata tattacaaaa ggttcattaa ttttctttat tacagcttgc tcaattaatg
    27961 tgtcatctga accataataa tattctccaa tacgatcaag aatttcttgc ttaacaggtt
    28021 gaatcaatga ttgtgcttgt tcttttgagt cggcatttgc agtcaatcta atatatactt
    28081 catgacttcc tgccaaaggc gcaatcgtag gattagtctg tttatcgata agatctatta
    28141 atactgtttc tactttagat tcacctattc cagcaaatct taatagttca gagtgtataa
    28201 ttcgattatg gtttataaaa tgtgacaaca attcattttt taccattggt tgcatttctt
    28261 tcggtggacc tggtaataaa ataatttgtt tgttttcaaa attcaccatc attcctggag
    28321 ccatgccatg atgatttgct aatacagttg aaccttcaat tactaaagcc tgttgtttat
    28381 tattaggtgt catttcttgt ccttgttcct caaaatagct ttcaatatat tttaaagaag
    28441 gctcatcaat aactaaatct ttaccaacaa tctgggccac tgtatgcttc gttaagtcat
    28501 ctttcgtagg acctaagcca cctgttaaaa taacagtatc atatttttct aacgcatgac
    28561 g
//
LOCUS       DAMZPP010000015        27592 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMZPP010000015 DAMZPP010000000
VERSION     DAMZPP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 27592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27592
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.015"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..638)
                     /locus_tag="POH73_000501"
     CDS             complement(<1..638)
                     /locus_tag="POH73_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDI6886479.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAIT"
     gene            complement(638..898)
                     /locus_tag="POH73_000502"
     CDS             complement(638..898)
                     /locus_tag="POH73_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HDI6886480.1"
                     /translation="MALLVNKRLLDEGKTIDVYLFEALNDQIIIAIPDWFWSYQMAMT
                     LNEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            complement(879..2363)
                     /locus_tag="POH73_000503"
     CDS             complement(879..2363)
                     /locus_tag="POH73_000503"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDI6886481.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQSVADQGCKLVVVNKEQSLSANVTQVVVPDTLRVASILAHTLFDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAENIQESL
                     QGVSFDFVTPFGTYQVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVGQ"
     gene            2795..3970
                     /locus_tag="POH73_000504"
     CDS             2795..3970
                     /locus_tag="POH73_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HDI6886482.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICQDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            3948..5138
                     /locus_tag="POH73_000505"
     CDS             3948..5138
                     /locus_tag="POH73_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6886483.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHIVWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIANR
                     EKAK"
     gene            complement(5251..5760)
                     /locus_tag="POH73_000506"
     CDS             complement(5251..5760)
                     /locus_tag="POH73_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HDI6886484.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIE
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(6502..7353)
                     /locus_tag="POH73_000507"
     CDS             complement(6502..7353)
                     /locus_tag="POH73_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="base excision DNA repair protein"
                     /protein_id="HDI6886485.1"
                     /translation="MDISLNFNHYIDLKKSIKNLCLMKKFEPYVTNENYDFIEMPLVV
                     DGNYVLLKIYQEKDALIKLHYQEVLNSNRKSVIQESDIVRISRSILNEALDINSNIKY
                     KSDTFGLTIISDADNYTTLIRTILAQQVSIEQANKLFLLFINTYGEIFELNNKEYTFL
                     NWDKIFSRIYKEQKVLVGTTNNRKQAIITLAELHIKNGLRENVLQVISSIKGIGSWTV
                     QMFMLFQFPHVAFENIPVADIGLHRAIEKNHDLPHKSIDKSKGESYFQNMKSIDVFKY
                     WYIYMVE"
     gene            complement(7355..8080)
                     /locus_tag="POH73_000508"
     CDS             complement(7355..8080)
                     /locus_tag="POH73_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886486.1"
                     /translation="MNNKLIYTSYDDDNILLIDSFIKLVIDFKYIPINPTKSLGYYIS
                     TSIHDNDKGECLRDCLSLEMICDELWVFIDNNKYIPEGVRLEIASWLKYKSSPVKYIS
                     IPSLLENSSINDDLFLDFDDSNILKEKEISEPVPKKSELRPVNCINILPEHHKYIDWI
                     KYHLFYNKFVPLDYLSIKPYIYFDNIEHYKSELSLLNERCNNISVMPYYVSEDNFNLS
                     FSECKIPKYIKKDWAITTMENKN"
     gene            complement(8246..9004)
                     /locus_tag="POH73_000509"
     CDS             complement(8246..9004)
                     /locus_tag="POH73_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDI6886487.1"
                     /translation="MLEFKAGKINKHVLYSDILNRDVTLSIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFEGGHQWKDWKPLLSDILLYFFSNQSPDQLYE"
     gene            complement(9147..10715)
                     /locus_tag="POH73_000510"
     CDS             complement(9147..10715)
                     /locus_tag="POH73_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDI6886488.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSVFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGSGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            11057..12142
                     /locus_tag="POH73_000511"
     CDS             11057..12142
                     /locus_tag="POH73_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI6886489.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            complement(12338..13108)
                     /gene="fabI"
                     /locus_tag="POH73_000512"
     CDS             complement(12338..13108)
                     /gene="fabI"
                     /locus_tag="POH73_000512"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDI6886490.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(13386..15230)
                     /locus_tag="POH73_000513"
     CDS             complement(13386..15230)
                     /locus_tag="POH73_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HDI6886491.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKTHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            complement(15240..16625)
                     /gene="mgtE"
                     /locus_tag="POH73_000514"
     CDS             complement(15240..16625)
                     /gene="mgtE"
                     /locus_tag="POH73_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDI6886492.1"
                     /translation="MSMNTDEKERVQEELYDETLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQDHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            complement(16646..17500)
                     /locus_tag="POH73_000515"
     CDS             complement(16646..17500)
                     /locus_tag="POH73_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDI6886493.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMPNDILEIHLPREIPSVNLIPYARKLEVLYEDAFVIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTFGIIEANIRRSKDSIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            complement(17497..18306)
                     /locus_tag="POH73_000516"
     CDS             complement(17497..18306)
                     /locus_tag="POH73_000516"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDI6886494.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            complement(18323..18958)
                     /locus_tag="POH73_000517"
     CDS             complement(18323..18958)
                     /locus_tag="POH73_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDI6886495.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            complement(18975..19322)
                     /locus_tag="POH73_000518"
     CDS             complement(18975..19322)
                     /locus_tag="POH73_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886496.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            19508..20101
                     /locus_tag="POH73_000519"
     CDS             19508..20101
                     /locus_tag="POH73_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDI6886497.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LEKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            20205..20570
                     /locus_tag="POH73_000520"
     CDS             20205..20570
                     /locus_tag="POH73_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HDI6886498.1"
                     /translation="MTTTPYDIIGKEALYDMIDYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHMAFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            20593..21399
                     /gene="yjbH"
                     /locus_tag="POH73_000521"
     CDS             20593..21399
                     /gene="yjbH"
                     /locus_tag="POH73_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HDI6886499.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRLRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(21859..23667)
                     /gene="pepF"
                     /locus_tag="POH73_000522"
     CDS             complement(21859..23667)
                     /gene="pepF"
                     /locus_tag="POH73_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDI6886500.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDENAHPLTQGTFIKYLESDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(23715..24701)
                     /locus_tag="POH73_000523"
     CDS             complement(23715..24701)
                     /locus_tag="POH73_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDI6886501.1"
                     /translation="MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHF
                     AHKHLAEQKCFNNESIKHYKSKLILAQMIQQQGCKVEIEPFLKEIKQIPDILINNKYV
                     IELQYSPIPYKQILQRTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFQSMFINPITRKL
                     HTFNLEKKQIIMFQQIQYLGGHKYVAEKKNAKISELFNEAPCDYHAVYKLSKFAINQY
                     IKYCRWQNSVLEPTLSAMYQLQLTDHEVVHNYGYIFPEQIYIENHPIEWQLQVDLWLK
                     NGKSKLVSDNLNYFKLKKFIVALESKTAIIEKLINNYLNISSDRGNDVQILF"
     gene            complement(24822..25541)
                     /gene="mecA"
                     /locus_tag="POH73_000524"
     CDS             complement(24822..25541)
                     /gene="mecA"
                     /locus_tag="POH73_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDI6886502.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVHFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
     gene            complement(25912..26307)
                     /gene="spxA"
                     /locus_tag="POH73_000525"
     CDS             complement(25912..26307)
                     /gene="spxA"
                     /locus_tag="POH73_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDI6886503.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
     gene            26602..27591
                     /gene="trpS"
                     /locus_tag="POH73_000526"
     CDS             26602..27591
                     /gene="trpS"
                     /locus_tag="POH73_000526"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDI6886504.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKVFLVEFQEKYESFYNSDKLDDILDQGRDKAHKASFKTVKKMEKAMGLGRKR"
BASE COUNT         9253 a         4835 c         3829 g         9675 t
ORIGIN      
        1 tttgtaattg catgatcttc ttccaactca aaatcatcat tcaaatgtaa tatattttct
       61 tcatctctaa atggttcaat tgttttagac tttctatcaa tgatgccaaa gaaactttgt
      121 cccataccaa ttggccaatt catagggtat gtttcaatat ttaatgtctc ttcgatttca
      181 tctaataatt caaatggttc tttacctact cggtctaatt tattaatgaa tgtaaaaatt
      241 ggaataccac gcattttaca aactttaaat aacttcaagg tttgtggttc aatacctttt
      301 gcacagtcta tgaccatgac agcactgtca actgccatta atgttctata cgtatcttct
      361 gaaaagtctt catgtcctgg tgtatctaag atattgattt tataatcatc gtaatcaaat
      421 tgcattactg aacttgttac agaaatacca cgctcttgtt caactttcat ccagtcactt
      481 gtcgcaaatt taccagtctt cttcccttta actgtacccg cttcacgaat ggcaccactg
      541 aagtacaata gtttttcagt taacgttgtt ttccctgcat cgggatgtga aataatcgca
      601 aaagtctttc tagactcaac ttcttgcttt aagttcatta gtctttctcc tttttatatg
      661 tttctatcca atctgttagt tgtgatgcaa tcgattctgc ctcttcctct tctttaaaaa
      721 caaacaattg catgagtatt gcttcaaaac aagtttcttc atttaatgtc attgccatct
      781 gatatgacca aaaccaatct ggtatagcaa tgattatctg gtcatttaat gcttcgaata
      841 aataaacatc aatagtttta ccttcatcaa gaagtctttt attgaccaac agggccacca
      901 ccgaactttt tgtaagctgc ttcaaggcca attaaatcat ctcgatgtgg caccttaata
      961 tgtcctggca tgatttgata tggttctctt ccttttgatg ctaaaacgac agtatcccca
     1021 ggctcagcta tgtcaattgc atgttttatc ccttctgcac gatcatcaaa ttctatatag
     1081 ttttgatgtg ttgcaccttt ggctaattct gctgttaaca ttttcgggtc atcatttgcc
     1141 ggattatccg gtgtgaaaat gacataatct gcacgacagg caactcgccc catttcaggc
     1201 gttttagtta aatcacgttc gcctgccata ccaactaaaa atatcaactt ttgctttaca
     1261 aaaggctgta ctgcatcgat taatttgttc ataccatcag ctgtatgtgc ataatcgata
     1321 attaaatcaa taggtaacga aggatctaaa acttctaatc gtccttcaac aggttctaaa
     1381 ttttcaacag ctttaataat cgtttctaaa gatgtacctt tactccacac cgcaatcatt
     1441 gctgccataa tattagaaat attaaactta ccaacataag gcgattttac ttggtaagtt
     1501 ccaaaaggcg ttacaaaatc aaagctgaca ccttgtaaag attcttgaat attttcagcc
     1561 ataaattggg cttcctcatc aattccataa ctaaatactt cataaggcgt cactgttctt
     1621 aaatactcag aaaatgaatc gtcattgttt aacacgacat acttttcttt cgacaaatct
     1681 tcacctaatt gactaaacaa taaagacttc gcgtgtccgt acgcttccat tgtgccatga
     1741 aaatctaaat ggtcttgtgt taaatttgaa aatattgcaa cgtcaaattc aacgcctcgc
     1801 agtcgtccta atactaagcc atggctcgat acttctaatg tcatggattc agcgcctgca
     1861 tcaactgctt ctttaatttt cttagtcaaa gaaactgttt ctggtgtcgt atttgcacct
     1921 tttgtctttg tttcattaat ttggaaacca ttagttccta aatatgcact atttttttgt
     1981 aactttcttt gaattaaatg aatcatcgtc gcaatagagg ttttaccatt tgtacccgtt
     2041 acaccaaatg tcactaactg atgactcgga taatcaaata atgtgtgtgc tagaatacta
     2101 gctactctta atgtgtccgg cacaaccact tgtgttacgt tagctgataa tgattgttct
     2161 ttattgacca ctaccaactt acacccttga tcagctacac tttgacagaa cttatgactg
     2221 tctacagtat atccaactga agcgacaaaa atgctacctt ctctcgctgt acgtgaatca
     2281 gtagtgatat catctatttg ttgttctaaa gaacccaata cacgctttac ttttactttc
     2341 ttaaacaacg tacttgcatc caaactgaac cgctcctttt tctacaatat gtttattata
     2401 cacgttttgt tgatgttaat aaatttataa tttcactgtt atatcctact aatcagcatt
     2461 ttctaatttc tactattaaa aataaaacat aaggtattat gaattagttt cacaattatg
     2521 gttcatctta aattattaaa accatcttgt aacatggtat aatatcataa ttgtttccaa
     2581 aaatatgcta taattgtata ctggataatt ttaaatatac aaataaaaca tagaacaata
     2641 aattgcttaa taaaataacg ttttattttg tatcaattca tctattgatt aaaatgctat
     2701 aaatgtaacg actttgcgtc tttatttctt ttataatgcc gtaaaataag ttacacttaa
     2761 ataaaatttt caaactaacg gagggtggct attaatggtt actcaaaata aaaagatatt
     2821 gattattact ggctctttcg gtaacggtca tatgcaagtt acacagagta tcgttaatca
     2881 acttaatgat atgaatctag accatttaag cgtcattgag cacgatttat ttatggaagc
     2941 tcacccaatt ttgacttcta tttgtaaaaa atggtatatc aatagcttta aatattttag
     3001 aaatatgtac aaagggtttt attacagccg cccagataaa ctagacaaat gtttctacaa
     3061 atactatgga cttaataaat taataaattt attgataaaa gaaaagccgg atttaatatt
     3121 attaacgttt cctacaccag ttatgtcggt actaactgag caatttaaca ttaatattcc
     3181 agttgctaca gtgatgacag actatcgctt acataaaaac tggattacgc cgtattcaac
     3241 aagatattat gtggcaacaa aagaaacgaa acaagacttt atagacgtag gtattgatcc
     3301 ttcaacagtt aaagtgacag gtattcctat tgataacaaa tttgaaacac ctattaatca
     3361 aaagcagtgg ttaatagaca acaacttaga tccagataag caaactattt taatgtcagc
     3421 tggtgcattt ggtgtatcta aaggctttga cacgatgatc actgatatat tagcgaaaag
     3481 tgcaaatgca caagtagtta tgatttgtgg taagagcaaa gagctaaagc gttctttaac
     3541 agctaagttt aaatctaacg agaatgtatt gattctaggt tataccaaac acatgaatga
     3601 atggatggca tcaagtcaac ttatgattac gaaacctggt ggtatcacaa taactgaagg
     3661 tttcgcccgt tgtattccaa tgattttcct aaatcctgca cctggtcaag agcttgaaaa
     3721 tgccctttac tttgaagaaa aaggttttgg taaaatcgct gatactccag aagaagcaat
     3781 aaaaattgta gctagtttaa cgaatggtaa tgagcaatta actaacatga ttagtacaat
     3841 ggaacaagat aaaattaagt atgctacaca gacaatatgt caagacttac ttgatttaat
     3901 cggtcattca tcacaaccac aagagattta tggaaaggtt cctttatatg caagattctt
     3961 cgttaaataa ttacgctaat cataaaaatt tcattttaat gcttattatc ttatttttaa
     4021 tggaatttgc gagaggcatg tatatcctaa gctatattaa tttcttgcca acagtaacat
     4081 ccattgctgt agcaataacg tctctagcat tttcaataca ttttattgct gatgcatcta
     4141 caaactttgt tataggattt ttactgaaaa aattcggcac aaaaatcgtt ttaacgactg
     4201 gatttatttt agcatttaca agtttatttt tagttatttg gtttccagca tcaccttttg
     4261 taatcatatt tagtgctatg atgctcggta ttgcagttag tcctatttgg gtcattatgt
     4321 tatccagtgt tgaagaagat aaacgtggca aacaaatggg ctatgtttat ttttcatggt
     4381 tgttaggcct attggtaggt atggttttta tgaatctact tatcaaagtg cacccaacac
     4441 gctttgcctt catgatgtct ttagtcgttt taattgcttg gatattatat tattttgtcg
     4501 atgtaaagtt aacaaattat aatactcgtc cagttaaagc tcaattgaga caaattgttg
     4561 atgtgacaaa gcgccattta ttgttgttcc ctggtattct tttacaaggt gccgcaattg
     4621 ctgcactagt tcctatatta ccaacatatg ctactaaggt tattaacgta agcacaattg
     4681 aatatactgt tgcaatcatt attggtggta tcggctgtgc agtttcgatg ctatttttat
     4741 cgaaactaat agataaccgc agcagaaact ttatgtatgg tgttattcta agcggattta
     4801 ttttatacat gatattaatt tttactctat ctatgattgt taatattcac atcgtgtgga
     4861 ttatcgcttt agctatcggt ctaatgtatg gcatcttatt accagcatgg aataccttta
     4921 tggcacgatt tatcaaatca gatgaacaag aagaaacttg gggtgttttt aatagtatcc
     4981 aaggattcgg ctcaatgatc gggccattat ttggcggatt aattacacaa tttaccaaca
     5041 atttaaacaa tacattttat ttctcggcat taatcttttt agtcttagct gtattttatg
     5101 gaagctactt tatagcaaat agagaaaaag ctaagtaagg ttaaaacgta ttttaaaata
     5161 aagaaaatgg tcatttttat tacattcaat aaaaatgacc attttcttat taattcaaac
     5221 atactacgat taacaatact attatgttac ttaagctaat ttaaacgttt caataacttt
     5281 ccatttatct tcgtcatcgt caaaacgtaa caaagttaat tcatcgataa tttctttatg
     5341 gtctacccca gctaatgcta cttgaccaaa aatatcttcg aattcttggc tagatagtcc
     5401 ttgtgctatt gtaaagtgtg gcacaaaaac atgttcagct tctccataga aatcttctcc
     5461 attaaagcga ttaaacaatt cttctaagtc gtccgtcttc gcaactttaa agtaaatcac
     5521 attgttcgtt ggtttgaagc tagaagcttt tgtagcatga acttctactg ctggtatacc
     5581 attaatacga gctctaacct gttcaatgac agaatctaaa tcaccatctt caatttcaaa
     5641 tggtgcttta attgtcacat gtggtttaat tcgtgaatac tgtttatcat atcttttacg
     5701 gtaagaatcc accgcttctt gaaatgactt tgatggaatt aatgctaatc ctaaaatcat
     5761 ctgaattcct cctttgttta acctattgct attatatcaa atttctttga aaaccgttac
     5821 attaaaaata attcattata catcttttgc aatttcgaca aatttaaatt aaattaaaca
     5881 gtaaaatctt ttataaatat atttaactat agcattgtca aatttatcta gacagcctag
     5941 ataaccctat aaataatttt aattctgttt tataattcag tgcgtttcta ttacaatgtt
     6001 catttaagct tgttgtgata ggattctata ataaacaaaa aatattgatt tctagtaaca
     6061 gttgtatatt atttttagtt aaagaactcc atacaacatt cgtatgtaat tggtttatag
     6121 atataaaaat gtatcgctga aaaaatatat tgagaatttt ctacaacaat taccttaaac
     6181 ctttccggta gtaatgtgat aatcatctta ataattcctt taaaaaaatg atagaactgg
     6241 catgataaat agtcactata ctgcctattt aagataatcc aacatttata taaataaagt
     6301 agtatcttct attatttcag aaataggtct ttaaaaagtt tatctactct tacctttcat
     6361 ttaaaaagta cgtaaagtaa acatattctg taaagtaatt tacagaatat tacatcggga
     6421 gttgaataat tagaaaagag attaagcaat ccttatattt aataggtttt atttttactg
     6481 tacaacaaac tgcttaaaca tctattcaac catgtatata taccaatatt taaacacatc
     6541 tatagatttc atgttttgga agtaagattc tcccttactt ttatcaatcg atttatgtgg
     6601 aagatcatga tttttttcta tggccctgtg caatcctatg tctgctacag gaatattttc
     6661 aaaagctacg tgaggaaatt gaaatagcat aaacatttgc actgtccaag aacctatacc
     6721 ttttatactt gaaataactt gtaaaacatt ttctcttaag ccgtttttta tatgtaattc
     6781 agcaagagtt atgatagcct gctttctatt attagtcgta cctactaaaa ctttttgttc
     6841 tttatatatt cgactgaata ttttatccca atttaaaaaa gtgtattctt tattatttag
     6901 ttcaaaaatt tcaccataag tattaataaa taataaaaat agtttattgg cttgttctat
     6961 gctcacttgc tgagcaagta tagtccgtat taatgttgtg taattatctg catcggatat
     7021 aatagttaat ccaaaagtat cagatttata ttttatatta ctattgatat caagagcttc
     7081 attaagtatg cttcgtgaaa ttctaactat atcactttct tgtattacac tttttcgatt
     7141 agagtttaat acttcctgat aatgaagttt aattaaagcg tctttttctt gataaatttt
     7201 taagagcaca taatttccat ctactaccaa aggcatttca ataaaatcat aattttcatt
     7261 ggttacatag ggctcaaact ttttcattaa gcataaattt ttaatactct tttttaaatc
     7321 aatataatga ttgaaattta atgatatatc catgttaatt tttattctcc attgttgtta
     7381 tagcccaatc tttcttaata tatttaggta ttttgcattc tgaaaaagat aaattaaaat
     7441 tatcttcact cacataatat ggcataacag aaatgttatt acatcgttca ttcaatagcg
     7501 ataactcact tttataatgt tctatattat caaaataaat atatggcttt atacttaaat
     7561 aatccaatgg tacgaatttg ttataaaata aatgatactt tatccagtct atatatttat
     7621 ggtgttccgg tagtatatta atacaattta caggtcttaa ctcacttttt ttcggtactg
     7681 gttcggaaat ttccttttct ttaagtatat ttgaatcatc aaagtctaaa aataaatcgt
     7741 catttatact cgaattctct aataaagaag gtatgcttat atacttaact ggagaggatt
     7801 tatattttaa ccatgaggct atttctaacc gaactccttc tggaatatat ttattattgt
     7861 ctatgaatac ccaaagttca tcacaaatca tttccaaaga caagcagtct cttaaacact
     7921 cacctttgtc attgtcatga atcgatgtag atatataata tcctaatgat ttagttggat
     7981 ttattggaat atatttaaaa tcaataacaa gtttaataaa actatctatc aatagtatgt
     8041 tatcatcatc ataagaggta tatataagct tattattcac tgtaaattcc ccccttcact
     8101 attctataca tttcaccttc ttgaagctct ttatccgttg ttattgcagg aatatgcttt
     8161 tctcttaata taatacctaa gtgccctaaa attgcccctc ttttgaagat aaaaccacta
     8221 caataatcag ttatataaga acatattact catataattg atccggtgac tgattactaa
     8281 aaaaatacaa taatatatct gacagcaatg gtttccaatc tttccattgg tgaccacctt
     8341 caaattcatc gtaataataa gttatattat atttcttaat ttgttcagct aattctctat
     8401 tcggtgttaa gaaatcggca cgcttaccat ttgttggtaa agtgaaatct ttttcatcta
     8461 gaccaatgac atgccaaatt gtcaattgtt ctttattagc acattgattt agcttatcta
     8521 atactttttc atctgaatgt ggacttaaca ttgctacacg actaaaaatc gttggatagg
     8581 tcaacgccgt taataaggca atactacccg ctaaactatc tcctactaat aaccttgcat
     8641 tacctacttt cagtgtagaa aacgtcgagt caataaatgg caatatttct ttaccgactg
     8701 attgaatagt taaatgagaa cgacttcctt gtggatgaaa ttcttctcta cgcttatcaa
     8761 cgtcttcata atggaatccg acaatgatcg catcatcaat acgcgcttct ttgattaacg
     8821 attcatatgt acgttgtatt ctcccgaaac gtaaaaaatc taatccgtca aagcaaagaa
     8881 tgacattata tttaacaagt tgattataag attctggcaa ataaatactt agtgtgacat
     8941 ctctatttaa aatatcacta tataaaacat gtttattaat ctttccagct ttaaattcca
     9001 acataaaaaa gtgcccccta gtaattgtat tattattgta ccaagagaca ctatatttta
     9061 aaattcaaat ttctgagcac ctaaaattct caatactttt tcttttttat caaatgatca
     9121 ctacatatac cataactaaa agttgactac tttttggcac gtgcttgttt taaacgttct
     9181 ggatatgtct tcaaccaaaa gacagcatta ggtaatttat tcggatcagg ttgataaact
     9241 gcattataac cgctgcctaa acgtttcttt tgaatttcat aatcttttaa agcatttacg
     9301 gcaggcttat gtaaaatcca aatcgcaatg atatttaacc aagccattag acctacacct
     9361 aaatcaccga atgcccatgc tacatcagct gttttaactg caccgtaaaa tgtagcgaac
     9421 aaaataatca cacgagcaat attaataaat accgatgaaa cttgattatt ttgattacgc
     9481 gttaaataag caacatttgt ttctgtaatg tagtagtacg acaaaattgt agtaaatgca
     9541 aagaagaata aagcaaatgc aataaagtac gaacctacgc cagagaaagt aggatcaaat
     9601 tgataaccac tgccatggaa cgctttatca atgccggctt gtgcatacat cgcagtacct
     9661 gaataatctt tacctgttgc attttcaaca taaatcccac cgtctttaat taaatgcggt
     9721 gtgccattcg cattaaccgt accatcagtc acattatatg taccagaaat aagtataatc
     9781 agagcagttg cagtacatac aaataatgta tcaatataaa ctgaaaatgc ttgtactaga
     9841 ccttgtttac ttggatgtga tacttctgct gccgctgctg cgtgtggacc tgtaccttga
     9901 ccagcctcat ttgagtataa tccacgttta acaccaatct ctatcattgc gccaacgata
     9961 ccaccaaaag cagattgtaa tccaaatgct gatttgaaaa ttaatgcaaa taacgctggc
    10021 acttcttgta tattgataca aatgataatg acagccatta gtatgtaaat aattgccata
    10081 aatggtacaa cggctgttgc aacattggca atactacgta caccaccaaa aataattaat
    10141 cccaaaataa ctacaacaat accacccatt aaccattgtg gaacatgaat cgcattgtgc
    10201 atagaacttg ctatagcgtt agattgcaca ccaggaagca atagacctac tgagataatc
    10261 gtaacaatag caaagataat tccgtaaatt ttaccaaatt taccaccaat accatattct
    10321 atataatacg ctggtccacc acggtattca ttattttcaa ctctcttgaa tatttgacca
    10381 agtgtagatt caataaaagc actacttgca cctaaaaatg cagtaatcca catccaaaat
    10441 actgcaccag gtcctcctat aaatattgca gtagatacac cgacaatatt acctgtacca
    10501 accctgcctg ctaaagacat ggctatcgct tgaaaacttg aaataccatt tggagacttc
    10561 tctccttgaa acattaatcg aatcatttct ttaaaatgtc taacttgaaa aaatcgcata
    10621 cgcaatgtaa atagcacacc agtaattagc aaaccataaa ctaatggctt actccaaaca
    10681 acctcattta aaaaggttac taatttttca atcataagat tccccctatt gtttaaacat
    10741 aaaaatatta tacgattaag cacgagacac ttcaatatat ttttcagaat attcttataa
    10801 atattaatat gatcatttca ttatttaaac acgtttaata ttaaaataag ttattcatat
    10861 aaattggtac tgtttgatcc atttgcagac ttacgagtca ttgaaaagtc tcacaaacta
    10921 ttgaaagtaa atatcttaaa atagaaagta aatggttaat ttaagtatat ttaaaaaata
    10981 ttaacctttt taagcactgc tatttaggat atactaaata ataactaagt ttagaaaaat
    11041 aggaggaaca tcgtttatgt taaacaaggt ttggttccga actggcatcg ctctgattat
    11101 gctgttcatt ctcatcaaac tatttatgga agtgcacgaa gtatttactc caatagctac
    11161 aatcatcggt tctgtatttc ttccattttt aattagtggt tttttatttt atatctgtct
    11221 accttttcaa aacttacttg aaaaagtcgg ttttccaaga tgggctagta taacaacaat
    11281 catgttagct ttgtttgcta ttatcggctt aattgttgcg tttgttgcac caatcatcat
    11341 ttcaaatatc aacaatttaa tcagtcaaac acctgcctta caaaaagaag cagagcaaat
    11401 tattaaattc gcactagctc aaatggataa attacctgaa gatgtaacaa gtagaattac
    11461 aaatatggtt aaatcaatgg gtgatggcgt tactaatatt ttatctaatt cattacagta
    11521 cattacatca ttaatttcaa ccatcttctt attgattatg gttccattct tcttaattta
    11581 tatgttaaaa gaccatgaaa aatttatccc agctgttgct aaatttttca aaggtgaacg
    11641 taaagtattt ttcgtagatt tattaactga tttaaacttt acattaaaat cttacattca
    11701 aggtcaagta actgtcagtg ttattttagg tatattctta tatatcggtt attcaattat
    11761 tgacttacca tatattcctt tattagtact atttgctggt gtcgcaaact taattccttt
    11821 cttaggttca tggttatcat ttgcacctgc agcaatactc ggtattatag atagtcctac
    11881 tacatttata tgggtttgta tcattacatt aattgcacac caacttgaag gtaatatcat
    11941 tacaccaaat gtaatgggta aatctttaag tatccatcct ttaacaatta tcgttgttat
    12001 tttagcagca ggagatttag gtggctttac attagttctg attgcagtgc cattatatgc
    12061 tgtacttaaa acggttgtta gtaatatttt taaataccgc caacgcatta ttgataaagc
    12121 aaacagtaat gttaaggact aattctgtgg atgtctttta ataatataag atactatcgc
    12181 ctcaaaagtt gaaactacag cttttgaggc gatttttttg tgcataaaaa aatcagtcag
    12241 atgaaatatc aaataatttt ccatcagtcc gattattata aaagcaaaaa agctttgctc
    12301 acatatataa taacgtgaac aaagctgttg aatgatatta tttaattgcg tggaatccgc
    12361 tatctacatg aatattttca cctgtaacgc cacttgataa atcacttaat aagtaagccg
    12421 ctgttttacc tacttctact tgatcaacat tacgttttaa aggtgcacgc tcttcgattt
    12481 ctttaagaat tgtattgaaa ccacccacac cttttgcact taatgtacgg attggaccag
    12541 ctgaaattgc attaacgcga atattatctg gacctaagtc taatgctaaa tatttaacat
    12601 ttgcttctaa actcgcttta gcaacaccca tcacattata gttttgaact gcgaattcgc
    12661 cacctaaata tgttgttgca acaatgctac caccttctgg cattaatttt ttagcttcat
    12721 gagcaacgat agttagtgag taagaactaa tgtcttgagc taacaagaaa ccttcacgtg
    12781 aagtttcaga aaaacgtccg cgtaagtctt ccatattagc aaatgcgatt gaatgatata
    12841 caccatcgat attgccaaca tctttaccaa tttgctcaaa accattaata acttcttcat
    12901 cgctttgaac atcaatttga tataagtgcg cttctggttg atttaattgt tctaataatt
    12961 tttcaagctc tttacggcta cgttctttac ggtaagtaaa tactaattta gcacctaatt
    13021 gatctaaaac tttagcgaca ccaaaagcaa tactacgctt attagcgatt cccatgatga
    13081 catatgtttt gttttcaaga tttaacatgt aagataactc cttataataa ttaatttaac
    13141 ggatagaata tctaatccca gttttaacta actttttatt cgtcaataca ttttaacact
    13201 attatgactt tttaataaag ttatttaaac gtattacaac gataaatgtt tgatttagtc
    13261 ctttctgtat caaacttgat ttacgtaaaa agcatgtatt tatcaaaatc atatacatac
    13321 aagcatttta aaatcattaa ttttacgtcg tactaaaata cgtctgcatt ttaatatcat
    13381 tattgttaaa aataaaattc taactcttgc ttcaattcat caacgtattc tttagcaccg
    13441 gtaacaatta agcgatcacc atatcgcaat tgtgtatctc catgcggaac aatcgactca
    13501 ttattacgga taatacgcac gaagatgatg tctcctccga atgggaaatt acgtaattga
    13561 atattttcat atttataatt taacatttga atttcatata gtgacgtttc aacattactt
    13621 aataaattca acatgttagg tgtttcaatt aaacctttta ataagatttt attacttaag
    13681 tagctactga aaatttcaat acctgaatca actaactctg tatcgtccgt tgtgctttca
    13741 agtctgcaaa tgacacgctc aacttgatgt gttttggcta atttagcaac ttttcggtta
    13801 atatcatcgt cattcgtggc acaaacaact atgtctcggt caaacagacc tagtcgttct
    13861 aaaacatctt gttcataatc agcaatttct atcatcgtga tatcatccga caattgacga
    13921 cgatcactca agtctttgcg ataatataat gtcacgtcat ataactgaga tgttaaattt
    13981 tgcgctatag gaatcgttaa ttgattttta ccaattaaac taacttcaat tttacggtta
    14041 aactcatctg gaactggaaa cagttttttg aaaataatcg gaacgaatac acatgtaatg
    14101 actgcgctta aaattaatat acctgacgtt tcagctgaaa tagcatttaa tctttctgaa
    14161 attttggctg cagctatcac gagcgataat gttgatgtta ataaaaatgc tgatgcaatc
    14221 gttgttttca tatcaaacca acgtcgaata aacatgactg gaattaattt tgaaatgata
    14281 aatgcaacga ttaaaatcgg tatgataatt agtaatttcg gttctttaat taatgaaggt
    14341 atgtttaaat ctacaccaac cattatgaag aaaataggaa taaaaaaccc gtaaccaaat
    14401 gagtctaact tttcaaccat ttcttcatct ggatttaata atgaaacaac gacacctgct
    14461 aagaatgcac ctaatatatt ttctgcgcca actccctctg ctagggctac taataatatt
    14521 attaatgcaa acaccgcacg aataccgatt tgcgtcgtac catccatcaa tttttgtaaa
    14581 aatgacattc ttttaaattg aacacctaaa atatatgaaa ttgctgtgaa aacaactaat
    14641 atacctatta accatattgt actgccgcct tgaccattga ttgcaccata gaccgttaat
    14701 aaaatcatag ttactaaatc cgcaagcact gctactaata ggataaattg ccctatagtt
    14761 gttctcataa tattcatttc ttttaaagtt ggaacaacta cgcctaagga aatagttgaa
    14821 atgataatga ccattaataa cacatcatcc actaatccaa gccatttaaa tacatacgct
    14881 aaaagaatcg aaataatcat aataaatgca aatacagtta acgctagatt aagatgccct
    14941 ggaattgagg attcatcatc attttgtcct tgacgtgcgc gtttatcttt tttaaaagct
    15001 ttaaaatcaa tttctaaacc acttaaaaac attaaaaaga taaagcctaa cgttgataaa
    15061 atatttagaa ttgaatccct ttctactata tttagaaatg aatttccaat cacaattccc
    15121 atcaaaattt ctgcaacaac aactggcaag aaattaatat ttaatcgatt gacaattatt
    15181 ggagttaaaa acgctgctaa aacaacaata actaaagata aaaactccat aactcctcct
    15241 taaattaagt aagccataaa tgatgtagct aaaccaaaat aaatcaacat actaataata
    15301 tcattaattg ttgtaataaa tggtccacta gccactgctg gatcgatatt caatttattc
    15361 atcaataatg gaatcatcga acctacaaac gtccccaccg tcatcgcaca agttaaactt
    15421 cctgcaacaa ttaatgctaa aagtggctga tgatatattg caacaataat tgtaaataat
    15481 attgttgaac atacaacacc cgataataca ccacttcccg cttctcttaa tgcaattcta
    15541 aatttacttt gctcattaat ttcccctgtc gtaatgttac gaactgaaac ggctaaagat
    15601 tgtgtacctg aatttcctga cataccacta ataataggaa taaacgctgc gagtagcgct
    15661 acattttcta atgttttttc gaatctccct aaaattgtcg cagtaatcat tcctaaaaat
    15721 gttaaaataa tcaaccatgg taaacgcttt aatgctgttt taatgattga atcattagtc
    15781 gaatcgatat ctgaaacccc ggctaaacga gagtagtctt cactagcctc ttcatccata
    15841 acgtctaaaa tatcatcaat cgtgatgatc cccagcaaat ggtcttggta atctataaca
    15901 ggtacagcca tgaaatcata gtctctcata acttgagcaa catcttcttg gtcgtctgct
    15961 acattcacac taatgacacg ttcgttcata atatcttcaa tatacgcatc attttcagct
    16021 acaattaaat ctcttagcga taaaacacct actaatttac catcatcatc aacaacaaag
    16081 ataacatata ttgtttctgc gtctggcgct tgtgctttga ccaataataa cgcttcttta
    16141 acaggcgtat gcgctttaag tgataaatac tccgtcgtca taataccgcc ggccgtatcc
    16201 tcatcatagt gaagtaatgc tttgatttca ttcgcgtcat ctttattcat caatgttaaa
    16261 agactagcaa cttttggttt agtcaactca tttaaaatat ctactgcatt atcgtatgac
    16321 atttcttcta atatgtgact agcgtatgtc gcattcatct tatcaaatag caactcatat
    16381 tcatcgtcat caatatctaa ttgatcaaag aaatttgcaa cctcttcagg tgataaatat
    16441 tgaaaaatct tttgtctatt ttcatcggta gtatcttcaa aatactcact ttgttcatat
    16501 gtgtgtaatg ctagaaattc atctctaaat tgatcaatat catcattttc taagtattga
    16561 tctaataatg tctcatcata taattcctct tgaacacgct ctttttcatc tgtgttcatt
    16621 gacatactta caccccctgt gtgaattaga gttgattgaa taatttaagt aattgcttat
    16681 aatcaattgt aatataaata ttgttcttat tgattggatg aacaaaatat atttgcgtac
    16741 attgcagtac ttgaccatga attttgtcat gaaaaccatc atacaaagaa tctcccacaa
    16801 ttggatgccc aatatgttga aaatgtacac gaatttgatg tgtacgtccc gtatgcaaat
    16861 gaactttaca caaactatat ttatcatttt gatttattac ttcataagat gttttagcgt
    16921 atttaccatc cgaggcaact tctctagtta taatactatc ctttgaccgt ctaatattag
    16981 cttcaataat accaaatgta tgggttttac catatacaag gcaagtatat atttttttca
    17041 agtttacttt agaaaataaa tgatggatgt gtccatattt agcgaatatc acaataccag
    17101 ttgtattacg atctagacgc gtaacaatat gtgggttaat attttcacca tgttcttgac
    17161 aatgatatag tacttgttcg attaaacttt catgaggatg ttctctcgaa ggcgtacaat
    17221 tttgattgtt tggtttagta actatgatga caaaagcatc ttcatacaat acttctagct
    17281 tacgagcata aggtattaaa ttaacactcg gtatttctcg cggcaaatga atttctaata
    17341 tatcatttgg cattaattgc ttacgcactg tgactggttc atcattaaca attaaagcgc
    17401 cattattttt aatggcgctc actgtcttct tagaaaaatc atgtcgtgct aaaaaagttt
    17461 taacagtttc ttgttgtgat atatgatact taaatttcat cgttcttcat cacttgatat
    17521 gaaagaatcg tgtacacgtt tccagaatgg gaatggtcta aaacgtgcaa acctcacttt
    17581 ttcatttgct acacggtatt gtattgaatt aacattttta tgtttgatac taacatgatc
    17641 tatcgtcatt ctaatggtat catgattaac tggtgatatt aaacatgtat gatgctttgg
    17701 taatacaagt ggtgatccta ccgttctaaa cacacgatta tttattgagg caatttctgt
    17761 aatttgcatt gcttcaagtg aaggatgtat aagtgcgcca cctagcgctt tgttataagc
    17821 cgttgaaccc gaaggtgttg atacacataa tccatcgcct ctaaatcgct caaagtgttt
    17881 ccctcttaag ttaacatcca caacaagtgt tgagccattt tcagttttca tcgttgcttc
    17941 atttaatgct aaataccttg tttcatagcc gttgtcgttg tatctcataa taatttcaag
    18001 caatggatat tcaatgacct gaaactctga attattaatt tcgatgatta atttttcaac
    18061 ttcatgaggt aaccaatccg cataaaatcc taaatgacct gtatgaacac caacaaatgc
    18121 cacttttgat aacatgtggc tatactgatg gaatgcttgt agtaatgtac catcaccacc
    18181 aactgaaata acaatttcag gattttcact atcctcaatc atgcgaaaat ctttcatata
    18241 gttcatcatt ttatgcttta aggcattaga cttggagtca cctttagtta aaattgtata
    18301 gcgcatgatt tataacacct cgttaatcat tttcatgttt tttagaacgt ttttgcgtgt
    18361 aatatttttg agcttcctga atttcatctt taatttcaga catctcttca tcaagtaaat
    18421 acgctgcttc tgccgctctt tccaaacgat gttgaatttc atccggataa gcaccatcat
    18481 atttatatcg taaagtatgt tcaatcgttg cccagaaatt cattgctaat gtacgaatct
    18541 gaatctcagc caatataaac ttttggcctt gtaatgtttc gattggatat tcaataatga
    18601 catgatacga gcggtaacca ctttctttag tgttacgaat ataatctcgt tcttcaatta
    18661 ctttaaaatc ttttctttgt cttaaaatat tgacgacaac atcaatatct tcaacaaatt
    18721 ggcacatcat tcttaaacca gcgatatcgt acatttcttc tcttaaccta tcaaatggta
    18781 tttgtcgttt gtttgcctta tctataatac tagcgattgg tttaacacga ccagtaacaa
    18841 attctattgg cgacgcttgt tcaccaactt catattgttt gcgcatgcct ttaagtttca
    18901 ctttcaactc atcaaccgct tgcttataag gtgttaagaa ctgatcccat tgattcatag
    18961 tgcttcaccc cgctttattc aatgtcgaat gtttcttcaa cggttgcaac aaattcctta
    19021 ccataattat tatttccttt tatgttgtct aaaatataag taagttcttg gtggaattca
    19081 gtcaattcta gttcatcatt gataataatt tttttagtca tgttttcatg caacatcgtc
    19141 catgtttctt gtacgaaaat gagataagca tacgctttac catcatctaa tgtatacgca
    19201 aaggcataat tatcactatc aacgagcatt tgtcctactt cagataatcc tttaatagaa
    19261 tcttctgtat aacaataaag tatgtcatct ttaattttaa tttcattaat atataaacgc
    19321 atgcttaatc ctcctcttat tccataacat tttaacacaa ttcaataata caaaacactt
    19381 acgaacacta tttttaatgg tcaaattaac attgataata gataaaggaa attactttta
    19441 aaattcaact ttgtcattca taatatagtt aagataaatt gaatttgata cgaggtgtat
    19501 acatatcatg gcaacaaatc atgaaataga atttaaacaa atgataactg catcaatcta
    19561 taataaattg caagagaaat attttaaaga cagtgtgctt tttaagcaag tcaattacta
    19621 tattgataca cccgatttca aattaaagga acatcgctct gcactacgca taagagttaa
    19681 agacaatcaa tatgaaatga ctttaaagac accagcaaag gttggtctat tagaatacaa
    19741 ctatatcgtc gacattaaac cagagatgaa cttgattatt tcaaacgaca atcttccaga
    19801 tgacattcgt caaattatag ttgaacagtt tggtgtaaag gatacaactt tatcaatact
    19861 tggcgcatta acgacatatc gccaagaaac taaatataaa ggtgatttac ttgttttaga
    19921 taaaagtgag tatttcgaca ctacggatta tgagttagag ttcgaagtta aagactataa
    19981 tcaaggttta gaaaagttcc aatctttatt aaatgaacta aacctcgaac atcatcaacc
    20041 tttaaataaa gttcaacgtt tttttaagaa aaaagaaaca ctttcaaaca atataaatta
    20101 aattggcaaa tatgcatatt tctttcaaat gtttaaaatc atgttatatt agttatatat
    20161 taacttaagt tcgtaacagt aaatttttga aaatgggtga ttaaatgaca acaacaccat
    20221 atgacatcat tggtaaagaa gcgttatacg atatgattga ttatttttac acccttgtag
    20281 aaaaggatga acgacttaat cacctgtttc caggagattt tgcagaaaca agtcgtaaac
    20341 aaaaacaatt tttaacacag tttttgggcg gtccaaacat ttataccgaa gaacacggac
    20401 atcctatgct aagaaaaaga catatggcgt ttacaatcac tgaatttgaa agagatgcat
    20461 ggttagaaaa tatgcagaca gcaattaatc gcgctgcgtt tccacaaggt gttggagatt
    20521 atttatttga gcgcttaaga ttaactgcta atcatatggt gaattcctaa aattaattgt
    20581 aggtgaataa acatggctgg agaattacga ataatggaaa ataagagtcg tgaagatatt
    20641 aatctatcac ctgtaagtaa aatcgaaatt tattcatttt tcgatccatt tagctccgat
    20701 tgcttcaaat tatcagcaat cttatccaaa ttaagaattg aatataatca atatatacgt
    20761 atcagacata tattaaatcc ttcgttaaaa gtattaacga aatgccaagc tcaaagtaca
    20821 tccaactttg ataacatcgc cctagcttat aaagcagctg agttacaagg tcgtttacgt
    20881 gccgaacgat ttatacattt aatgcaaaat gaaatcatac ctaaaagaga tattattaca
    20941 gaatcaatga tttgtgactg tattcaaaat gcaggtattg atttagaagt atttaaagac
    21001 gacttacaaa aaagtaaatt aaccgaaagc ttgaaaattg atttgcatat tgcaagagaa
    21061 atggaaatcg aacaagcccc ttctctcgtt ttctttagtg aagatgttca tgaagaaggt
    21121 ttaaaagtcg aaggattata cccatatcac atctatactt atataattaa tgaattgatg
    21181 ggtaaaccta tcgaaaagaa tcttcctcct aaattagaaa cttatataca gcaacaacaa
    21241 cttgtaacga tggaagaatt acttactatt tatgaatggc cagaaaaact tttaaacaaa
    21301 gagttaaaga agttagctat tcaacaaaaa attgaaaaac ttaaatatcc agatggcgat
    21361 ttctggaaat ctaaaatgcc taaaatcaaa tcaaaataaa taaattaaag acctcgtaca
    21421 ccataaatgt aacgaggtct ttttattgac tcttaatttt gcaacattaa attattagaa
    21481 cgtaatgtta cgcatcgatc taaatatatt agtgatgttt ttcgaattta accagttcga
    21541 aaattataca aattatgaat ttggtacata atagacatat attaatcgtt aatgtataaa
    21601 aagtgcatat ttaatacatt tttatacaac ttcacttccc tcttattaaa aaagaacata
    21661 aaaaaacgtc gtgtctgaat acacgacgct aaacaaaggg gatgggagaa atttttcact
    21721 tcaaacaaag gggtatgttt gttatgtgat taatttcatg ttcataatat aacacgctat
    21781 ctctcccatt tcaacctttt aatagtactt aatttaaatt gtcacaatct gttcataatg
    21841 taagcgaata caacagtgct aagctttcat taatttttca aaagcgttca atttttgttc
    21901 aaaaacttca caagcttgtt caattggttc aggtgttgtc atatctacac cagcattttt
    21961 taatatctca attggataat ttgagctacc ttttttcaag aattcattaa tatatctatc
    22021 tactgctggc ttaccttctg ttaaaatttg atgacttaag ctttgagctg cactgtaacc
    22081 agttgcgtat tgatatacat aataattcat atagaagtgt ggaatacgtg accattcctt
    22141 actaatatct tcatcagttt ctacagaatc accgaagtat aatttattta atttggcata
    22201 ttcttcattc attctagttg gcgttaatgg ttcacctgct tcttcaatgg catgaatttt
    22261 atgctcaaat tctgcgaaca ttgtttgtcg gaataatgta gctctgaaac gttctaattc
    22321 ttggtttaat aataataagc gtttttcatc atcaagatgt ttatccatat aatcacttaa
    22381 aagtgcttcg ttacaagttg atgctacttc agcgacaaaa atagtgtagt cactagaatt
    22441 tgacggttgg aattttctac tgaagtaact atgtgctgaa tgtccaaatt catgaactaa
    22501 tgtgtataag tctgaaatag tattagacca gttaagtaga ataaatggat tagttaaatg
    22561 tgcacctgat gaatagccac ctgaacgttt acctttattc tcatagacat cgacccaacg
    22621 attgtttaag ccttctttaa ctacatttaa atattcttca cccattggtt ctaaagcttt
    22681 tagcatccac tctttagctt cttcataagg catttcaaac ttaatatctt taattaatgg
    22741 tgtatataaa tcatacattt tcaagtcatc taaacctagc aattctttgc gcaattcagt
    22801 atatctatgt agcaatggta aatatttatg tacagttttt actagattgt catatacatt
    22861 ttctggaata tgattattac tcaatgcttt ttctcttgca gttttgtaat tgtgtgtacg
    22921 agcattaaat acatttttct tcacttcacc tgctagcgta gcgccaagcg tattattatg
    22981 agcaccatat gctttatata catttctaaa agcactttct cttagtttgc gatcatctga
    23041 ttctaaatac ttaataaatg taccttgtgt taacgggtgt gcattttcat ctttatcaat
    23101 cgcatcttca aatactaaat cagcgttgct aaacataccg tatacatttg atggcgttga
    23161 taacgcgtcc tgcgcttctg ttaataactt ttccgtttca gcatctaaaa tatgaggacg
    23221 tttttcatta atcaacttca aatcaaatgc gaatttttgt aatttatcat atgaatttac
    23281 aaatgattga attttatctt catcaatttg taaaatctct ggcactaaga aactccatgc
    23341 cgagctaaat ttaataatta attgatgtgc tcttgactcc ataccagtat acttgtcgtt
    23401 cgttgtatct tggtcttgtt ttagatgcgc gtatacatat actttttcta atttagtacc
    23461 taatgtatct tctaattcta acgcattgta taatgtctca gcactatcac caatgtgtcc
    23521 tttaaattgt tcttctttgc ctaactcatt ttcaacttct ttaaatgcag cctcaaaagc
    23581 ttcatcatct ttgaaaattg ttgttaagtc ccatgtatac tcaggatatt tacgttcctg
    23641 ttcttctctc gataattgtt gactcatatg tagtaaacct cctaataaaa tataattgat
    23701 ataattttct cattttagaa caaaatttgc acgtcattac ctctatctga actaatattt
    23761 aaataattgt taataagttt ttctataatt gctgttttac tttctagagc aacaataaac
    23821 tttttcagtt taaaataatt aagattgtca cttactaatt tgctttttcc attctttaac
    23881 cataaatcaa cttgtaattg ccactcaatt ggatgatttt caatataaat ttgctctgga
    23941 aaaatataac cataattgtg cactacttcg tgatcagtta actgtaactg atacattgca
    24001 cttaaagtgg gttctaaaac agaattttgc cagcgacaat atttgatata ttgattaatt
    24061 gcgaactttg ataatttata aacagcatga taatcacaag gcgcctcatt aaacaactca
    24121 ctaattttgg cattcttttt ttcagcgaca tatttgtgcc cgcctaaata ttgtatttgt
    24181 tgaaacataa ttatttgttt tttctctaaa ttgaacgtat gaagttttcg agtgattgga
    24241 ttaataaaca tactttgaaa atgattgaac ttcactttat tatgacaata atcaacatca
    24301 tttaataacc aacttacttt atatcccatt ttctttaaac cttccgttcg ttgaagaatc
    24361 tgtttataag gaattggcga atactgtagc tcaataacat atttattatt aatcaaaata
    24421 tccggaattt gttttatttc ttttaaaaat ggctctatct ctactttaca tccttgttgc
    24481 tgtatcatct gtgctaaaat caatttactt ttataatgtt taatcgattc attattaaaa
    24541 catttttgtt ccgctaaatg tttatgtgca aaatgactaa ttactttgag cccacgcttt
    24601 aaaataactt gcttgccaca caccggacaa aaatattgtg tctttctcaa tgcagtagtt
    24661 gctaacacgc gttccttttc ttcatttaaa gctactaaca tatcttcacc tcaaaatatt
    24721 acacgtaagt ttaactacta ttactttttc agcttttgaa acaataaaaa aaagagactc
    24781 caaagagtct cctttcatag tgcgttgctt aattaatatc gttattcagt tgtctccgga
    24841 aaataacgtc gaacttgagc tgttacgtta tgactcataa ttattttagc atagtcattt
    24901 aaataaactt ctgttctgtc tgttggataa gcaaattcaa gcaattgact gtaactatca
    24961 ttaatgacct cttgatcaac atgactatca aaatgtacag cataataata agtaccatca
    25021 accatatata acaaatcttc aaactctgta gttattggat tgctatgata tgcataatta
    25081 ataacatctt ctaaatcgtt aaatttcaca ataattgttc ttgtattttt acgtgctgaa
    25141 gacttttgac gtttagaacc ttgagcctct ttttcttttg ttcgttgctc gaataattct
    25201 tctaattgat cttcaccttc taatgtttga gctaacaatt cttgaacttg ttcatcaaat
    25261 tgatcagttg catcatcatc agacatattc atcatatctt catttttaga tttagaaatt
    25321 gtgacttcga cacctttttc aaaggcatgt acttgaatcc ataatggacc ttctacaaca
    25381 aaatcttctt cttcgttaat ttcatccatc attgaccaaa agaattcttc gccacgtttg
    25441 cgatttgtcc ataaatcttc acgactaaat ccacgggcct cgatatcgct atatgttata
    25501 aacaatttta cagttgtatc atctactcgt tctattctca tatcatctca ctccttacag
    25561 tcgatgaata gtatccacat tgtattaaag catcaaacaa aatacaatat atttgtttga
    25621 tttctttctc taaaatattt tggtaactta taataacata ttcgttgtag caaaactatt
    25681 taaatgattt atacagttaa agcattaaag cactttctta atttaatttt accttatcat
    25741 agcaaatgtt tcatccaaaa cactctaata tagagctata ttaacaattg cacatttgat
    25801 ttaaaagata ctaagcaatt attgtaaatt taaaaccaaa aaagcaacac tatagaaata
    25861 gcgttgcttg acatatcttt tagtaaagta tgacacatag ttaaatggtt attagtcaac
    25921 catacgttgt gcttcttgta attggaacgt acgaactttt ctaggtaaga aacgtcgaat
    25981 ctcgtcctca ttataaccaa cttgtagtcg tttattatct aaaataattg gacgacgtaa
    26041 taagccagga ttatcttgaa tgattgaata taagtcttgt aatggtagtg aatcaatatc
    26101 aacatttaat ttttggtatg ttttagaacg tgtagaaatg atttcatcag taccgtcttc
    26161 agtcattttt aatatttgct taatttcatc aattgttaaa tgttcagaaa aaatattacg
    26221 ctccgtatac ggaatgtcat gttcttgtaa ccatgctttc gctttacggc aagatgtgca
    26281 acttggtgaa gtaaataatg ttaccataca tctcactctc ctatttgaat gaataaaatt
    26341 cattgcttaa aatttagtta tagatcaaga aaaaacattt ttttctaaaa ttcttaatcg
    26401 ttactattta ttataactat ctaacattaa aattaaatga gaaaaaccta atttttcaga
    26461 taagtttcta cacttataaa aaagattatt aatccctttg ttagtagcaa gttatacata
    26521 tattctaaca catcttacat ttttaagaaa tactgttata atgataattg ttaaattatt
    26581 actaagaaag taggcattta aatggaaaca ttattttcag gcattcaacc tagtggaatt
    26641 cctactattg gaaattatat tggcgcatta aagcaatttg ttgatgtgca aaacgactat
    26701 gattgttatt tctgtatcgt agatcaacat gcaattacaa tgccacaaga tcgcttaaaa
    26761 ttacgtaaac aaactagaca attagcagcg atttatttag cttctggtat agatccagac
    26821 aaagcaacat tgttcataca atctgaagtc cctgcacacg tacaagcagg atggatgtta
    26881 actacaattg cttctgttgg cgaattagag cgtatgacgc aatacaaaga taaagctcag
    26941 aaagcagttg aaggtatacc agctggtcta ttaacatatc cacctttaat ggcagctgat
    27001 attgttcttt acaatactaa tatcgttcca gttggcgatg accaaaagca gcacattgaa
    27061 ttaacacgta atcttgtaga tagatttaat agtcgttata atgatgtcct tgtgaaacct
    27121 gaaattcgta tgcctaaagt cggtggccgt gttatgagtt tacaagatcc aacaagaaaa
    27181 atgagtaaaa gtgatgacaa cgctaaaaac tttatttcat tattagacga accaaatgtt
    27241 gctgctaaaa aaattaaaag tgctgtgact gattcagacg gtattattaa attcgaccgc
    27301 gacaataaac caggtattac aaatttaatt tcaatatacg ctggattaac agacatgcct
    27361 attaaagata ttgaggcaaa atatgaaggc gaaggttacg gtaaattcaa aggcgacctt
    27421 gctgaaatag ttaaagtatt tttagtagaa ttccaagaaa aatacgaaag tttctataac
    27481 tcagataaac ttgatgatat tttagatcaa ggtagagata aagcacataa agcttcattt
    27541 aaaactgtca aaaaaatgga aaaagcgatg ggtttaggac gtaagagata aa
//
LOCUS       DAMZPP010000016        26846 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMZPP010000016 DAMZPP010000000
VERSION     DAMZPP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26846
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.016"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..127
                     /locus_tag="POH73_000527"
     CDS             <1..127
                     /locus_tag="POH73_000527"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and acetaldehyde from
                     2-deoxy-D-ribose-5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HDI6886505.1"
                     /translation="KASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADSDY"
     gene            155..1333
                     /gene="deoB"
                     /locus_tag="POH73_000528"
     CDS             155..1333
                     /gene="deoB"
                     /locus_tag="POH73_000528"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDI6886506.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(1464..2279)
                     /gene="phnE"
                     /locus_tag="POH73_000529"
     CDS             complement(1464..2279)
                     /gene="phnE"
                     /locus_tag="POH73_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDI6886507.1"
                     /translation="MTQEIEKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIESLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIL"
     gene            complement(2276..3076)
                     /gene="phnE"
                     /locus_tag="POH73_000530"
     CDS             complement(2276..3076)
                     /gene="phnE"
                     /locus_tag="POH73_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDI6886508.1"
                     /translation="MPLETPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            complement(3078..3851)
                     /gene="phnC"
                     /locus_tag="POH73_000531"
     CDS             complement(3078..3851)
                     /gene="phnC"
                     /locus_tag="POH73_000531"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDI6886509.1"
                     /translation="MSQIKFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSKIYGRTIKEDEKLGVN"
     gene            complement(4065..5021)
                     /locus_tag="POH73_000532"
     CDS             complement(4065..5021)
                     /locus_tag="POH73_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6886510.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            5250..6794
                     /locus_tag="POH73_000533"
     CDS             5250..6794
                     /locus_tag="POH73_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6886511.1"
                     /translation="MKNIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQQTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVNYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASGLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQTEKVSFDIPQNVSVNEPFEVTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNATIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            6845..8380
                     /locus_tag="POH73_000534"
     CDS             6845..8380
                     /locus_tag="POH73_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDI6886512.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVQYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFATLQQHLPEMKRYADIIVVCYHGGFEKDIESGTPTEVLTGENEGYGMLE
                     VFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGKVVLSTDEYENVSVESCELL
                     PVIDDPTFTIDEDDQHIRKQLEDWLDYEITTLPYDMMINHAFEARVAPHPFTNFMNYA
                     LLEKSGADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGFKLKQAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNKLMRIPQVVDFK
                     VEK"
     gene            8537..9304
                     /locus_tag="POH73_000535"
     CDS             8537..9304
                     /locus_tag="POH73_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886513.1"
                     /translation="METIKQEVRESIVKGIFAGYKDYVNERNEVKKRMVISDAYVYTK
                     SNHIENQVAKHLENFVKYIKENAGPSWKYLKFIFNKDEEKNNIMFILKNEDYFDEKNV
                     SVGKSLVADKNQKSKNYLEGLIAKNSGINFDTVGEDFKIRHQMTADSILFNIKEGSNR
                     DINSYFLIITYRIDKESKQLAAIKQWLPNPETNSAIMVDDLTPLLDKVIVEREDYHID
                     EEELEVLKNDGELEFIDVEHAFGISIDEDNDMIESER"
     gene            9310..10485
                     /locus_tag="POH73_000536"
     CDS             9310..10485
                     /locus_tag="POH73_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDI6886514.1"
                     /translation="MFIGKNLEYVRKLNALSRKELSEKINVSEQAIWQYETKNMMPEI
                     NKIYDMTSIFNVKSSYFISEQPEELLINSVDKHSIAFRAKNYKVSTKLLNKQYYQAMY
                     LSNLTSYLFSFVKIPDNIILSLINNLDDLLNGNLESLNKKESIKETAKVVRAKILQDE
                     SNEALLFMLEKAGIVIYEKRINDSIDAYSFWSKDQTPFIILGTNKGVAVRRNFDLAHE
                     LGHLVLHRHIQFDLLSPEEYKTIEHEADIFASEFLLPEEAFKKDFDQMTKKSNPDYLA
                     VLKEKWYVSIQAIAMRAYYLGLMSSTQYRYFWASLNKKGYKSKEPLDDVIEMSRPVKM
                     NSLLKLYFDRNILTPQKLLNYLKVDETFLNHLAGINLKLFKDYVNENREYNITNLYK"
     gene            10871..13480
                     /gene="adhE"
                     /locus_tag="POH73_000537"
                     /gene_synonym="adhC"
     CDS             10871..13480
                     /gene="adhE"
                     /locus_tag="POH73_000537"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDI6886515.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEIN
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTSIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQRTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
     gene            13825..14493
                     /gene="capA"
                     /locus_tag="POH73_000538"
     CDS             13825..14493
                     /gene="capA"
                     /locus_tag="POH73_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HDI6886516.1"
                     /translation="MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKY
                     QANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSK
                     LSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKA
                     DGTAVKVAPKTVVNLIGALFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSI
                     QKFN"
     gene            14509..15195
                     /gene="cap8B"
                     /locus_tag="POH73_000539"
     CDS             14509..15195
                     /gene="cap8B"
                     /locus_tag="POH73_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HDI6886517.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKSLIIDGDMRKPTQNYIFNEQNNNGLSNLII
                     GRTTMSEAITTTEIDNLDLLTAGPVPPNPTELIGSERFKELVNLFNERYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKTLMEKAGSNILGVILNKAKVDKS
                     SSYYHYYGDE"
     gene            15198..15962
                     /gene="cap8C"
                     /locus_tag="POH73_000540"
     CDS             15198..15962
                     /gene="cap8C"
                     /locus_tag="POH73_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HDI6886518.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMLDLLRQATTQGVTEVIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVITGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            15982..17805
                     /gene="cap8D"
                     /locus_tag="POH73_000541"
     CDS             15982..17805
                     /gene="cap8D"
                     /locus_tag="POH73_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
                     /protein_id="HDI6886519.1"
                     /translation="MAHLSVKLRLLILALIDSLIVTFSVFVSYYILEPYFKTYSVKLL
                     ILAAISLFISHHISAFIFNMYHRAWEYASVSELILIVKAVTTSIVITMVFVTIVTGNR
                     PFFRLYLITWMMHLILIGGSRLFWRIYRKYLGGKSFNKKPTLVVGAGQAGSMLIRQML
                     KSDEMKLEPVLAVDDDEHKRNITITEGVKVQGKIADIPELVRKYKIKKIIIAIPTIGQ
                     ERLKEINNICHMDGVELLKMPNIEDVMSGELEVNQLKKVEVEDLLGRDPVELDMDMIS
                     NELTNKTILVTGAGGSIGSEICRQVCNFYPERIILLGHGENSIYLINRELRNRFGKNV
                     DIVPIIADVQNRARMFEIMEMYKPYAVYHAAAHKHVPLMEDNPEEAVRNNILGTKNTA
                     EAAKNAEVKKFVMISTDKAVNPPNVMGASKRIAEMIIQSLNDETHRTNFVAVRFGNVL
                     GSRGSVIPLFKSQIEEGGPVTVTHPEMTRYFMTIPEASRLVLQAGALAEGGEVFVLDM
                     GEPVKIVDLARNLIKLSGKKEDDIRITFTGIRPGEKMYEELMNKDEIHPEQVFEKIYR
                     GKVQHMKCNEVEAIIQDIVNDFSKEKIINYANGKKGDNYVR"
     gene            17795..18823
                     /gene="cap8E"
                     /locus_tag="POH73_000542"
     CDS             17795..18823
                     /gene="cap8E"
                     /locus_tag="POH73_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HDI6886520.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKRFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            18836..19945
                     /gene="cap8F"
                     /locus_tag="POH73_000543"
     CDS             18836..19945
                     /gene="cap8F"
                     /locus_tag="POH73_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HDI6886521.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNNRNVELTLNYVDDIVAEIKRAIEGNPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDNEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            19949..21073
                     /gene="cap8G"
                     /locus_tag="POH73_000544"
     CDS             19949..21073
                     /gene="cap8G"
                     /locus_tag="POH73_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HDI6886522.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGF
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKIPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIIGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            21076..22155
                     /gene="cap8H"
                     /locus_tag="POH73_000545"
     CDS             21076..22155
                     /gene="cap8H"
                     /locus_tag="POH73_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8H"
                     /protein_id="HDI6886523.1"
                     /translation="MRIAILGATNIKHMSLLSHYLNHIDLNINEVDIIYTDKYDIEEN
                     IQGINNYYKYKVDIKEDWTFIKKAIAYYRFRPYAMKILKENHYDFVIVWGSYTGHLFK
                     SFLEKHYKNKFILNIRDYFFENNKLIKYRMKKIVDASRLTTLSSEGFLKFLPKSEKYR
                     IIYSYNMSIIRESNVTDEFKKRWPINIGFIGNVRFNEINQKLIKELANDSRFHMQYFG
                     TGSEKLEVFARENFINNITFSGGFDLEETPKYLNEIDILNNLFGNKNIALDTALSIRM
                     YYALFLNKPIITTDDTFTATEANKFGLGFSVNPENLKGIGDELMDWYNNLNVMDINHK
                     REAYRNDVIENNKQFYQEIGRIFNE"
     gene            22148..23542
                     /gene="cap8I"
                     /locus_tag="POH73_000546"
     CDS             22148..23542
                     /gene="cap8I"
                     /locus_tag="POH73_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8I"
                     /protein_id="HDI6886524.1"
                     /translation="MNKIYNVTSYVIAILMFPCLMLGDKPLLFLAPISYGVGKLFISF
                     SNNPNFKFSKIVYDVLGFLRLVFIPAMIVFFQDSTIDNLPLGQDYFNQAVIYMSVEFI
                     IGSLFILILSKLFKHEVVSRNSFTLSGSSIYYIVFGLVICGIFVAFPEVRKNISFLII
                     KTDAMGRGTEATSGLNVLFVMLFQLALALLFLIIAYASYKKYKENPKIIYVILPLAIG
                     ILNISLIVGERRSYQLYTMVAVLTVVSLLFSKHKRRINIIIISVGIFVLALMTLYKEL
                     YVFNYSSYSEALNSTSVSNLKIVDTLQSYFYGPSNIAASIDYLNYYNGSFKQYLFDNT
                     RAVFGFNFFVDKKQLITSQLFNQLIYGSKQLTGHLISSAGYGIIYFGPLFFYLNLIAN
                     IFFAFLSEYIIRKSHSLEVIFIGTYIYMRLITSIFSHPTPLITLISMILVVYVIAIIP
                     GIIIKKFTKKVGIE"
     gene            23539..24096
                     /gene="cap8J"
                     /locus_tag="POH73_000547"
     CDS             23539..24096
                     /gene="cap8J"
                     /locus_tag="POH73_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8J"
                     /protein_id="HDI6886525.1"
                     /translation="MIVKTFMKSKIFRLMNTPLLLFYKKEYLTGYYFENKVAGWLWAW
                     KAVPFKLLGINTSLPFPADITVRMHNPNNIVFDKNDIHIFQSPGTYFNNFSAVIYIGR
                     GVYIAPNVGIITANHNIKNLKSHAPGEDVKIGNYSWIGMNSVILPGVELGEHTIVGAG
                     SVVTKSFPEGNVVIGGNPAKVIKKI"
     gene            24105..25343
                     /gene="cap8K"
                     /locus_tag="POH73_000548"
     CDS             24105..25343
                     /gene="cap8K"
                     /locus_tag="POH73_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8K"
                     /protein_id="HDI6886526.1"
                     /translation="MRLNKFIGDSFLMILSSGIAQVILIITTPIITRLYSPTEFGEFT
                     IFSNIAMILIPIINARYDLLIVNTKNDRSANILSQISFLISLLILLILIPIFAISAWL
                     YPNFILDFIFIIIMLFLVSLTNIFTNYLNKERKYKVLSLINVFRAGSMALLQIIFGLL
                     ALGSLGLIIGFSLSYIAGITLGYKTFKKHFNIVRDKEETKALFLENKNQLVYSTPSIL
                     LNSLSFSVVVFFIGILYTNTEVGIYGMAIRVLGIPVTIISLGLSKIFMQQANDYYIEY
                     GNFRNLLLKFSSILVIVSIILYVPLYLFSEELVNILLGHSWVDAITVIKIVIPLFVIR
                     LIVSTVSLSVIVLQKQQLELILQALFLIGTTATFVISKMLNLTFLNFVSINTVVLIVS
                     YMIFFIALYYFAKNKQFKNS"
     gene            25377..26582
                     /gene="cap8L"
                     /locus_tag="POH73_000549"
     CDS             25377..26582
                     /gene="cap8L"
                     /locus_tag="POH73_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HDI6886527.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NKHVYKNAENVIVLGTEMKNYLLNHQISKNPDNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQPQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKPLVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            26593..>26846
                     /gene="cap8M"
                     /locus_tag="POH73_000550"
     CDS             26593..>26846
                     /gene="cap8M"
                     /locus_tag="POH73_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HDI6886528.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTAYITKTGKVIR"
BASE COUNT         9566 a         3884 c         4929 g         8467 t
ORIGIN      
        1 aaaagcatca ggtggcgtac gtaatttaga agatttcaat aaaatggttg aagcgggtgc
       61 gacacgtatt ggtgcgagcg caggcgttca aattatgcaa ggtttagaag cagattcaga
      121 ttactaatat atataaaatt tgggagtgat agctatgaca agaccattta atcgtgtaca
      181 tttaatcgta atggattcag taggtattgg tgaagcgcca gacgcagctg attttaaaga
      241 tgaaggttca catactttaa gacatacctt agaaggcttc gatcaaactt taccaaacct
      301 tgaaaagtta ggtctaggga acatcgataa attaccagta gtaaatgcag ttgaacaacc
      361 agaagcatac tatactaaat tgagtgaagc ttcagttggt aaagatacaa tgactggtca
      421 ctgggaaatt atgggattaa atattatgca gccttttaaa gtatacccta atggattccc
      481 tgaagagtta attcaacaaa ttgaagaaat gacaggtcgt aaagttgttg ctaacaaacc
      541 ggcatcgggt acgcaaatta tcgatgagtg gggcgagcac caaatgaaaa ctggtgactt
      601 aattgtttat acaagtgcag acccagtatt gcaaattgct gcacatgaag acattatccc
      661 attagaagag ttatatgata tttgtgaaaa ggttcgtgag ttgacaaaag accctaaata
      721 tttaattggt cgtattatcg cacgtccata tgttggtgaa ccaggaaact ttacacgtac
      781 atctaatcga catgactatg cgttaaaacc atttggtaaa actgtcttag atcatttgaa
      841 agacggtggt tatgatgtca ttgccatcgg taaaattaat gacatttatg atggtgaagg
      901 tgtaacagaa gcggttcgta caaagagtaa catggacggt atggatcaat tgatgaaaat
      961 tgttaagaaa gatttcacag gtattagctt cttaaactta gtagactttg atgcattata
     1021 cggtcatcgt cgtgataaac cgggttatgc acaagcaatt aaagatttcg atgatcgctt
     1081 gccagaactg tttagcaact taaaagaaga cgatttagta attattacag cagaccatgg
     1141 taatgacccg acagcgccag gtacggacca tacgagagaa tacatcccag taattatgta
     1201 cagtccgaaa tttaaaggtg gtcatgcact agaaagtgat actacattca gctctatcgg
     1261 tgcaactata gcagataatt tcaacgtaac attaccagag ttcggtaaaa gttatttaaa
     1321 ggaattaaaa tagaataaat ttagatataa taaaaacagc agtgaagtta actataataa
     1381 tagttttctt cactgctgtt tttattataa tagagaaacg taagacggta ggacctctta
     1441 ttcaggagta tcctgattta atgttaaaga atacgttttc ggattgaacc ggaaattaaa
     1501 tcgacaattg cgaccattag tactaaaccg attaatataa tacctacacg gtcccaagaa
     1561 cgtgtttgaa tggcaaatat gagtggtgtc ccgataccac cagccccaat tagccccagt
     1621 atagaagctg aacgtaagtt tagttcaaag cgataaagta tgagtgatag aaaggcaggc
     1681 ataatttgtg gtatgactgc aaatacgagt gttttaatct tattcgcacc actggccttt
     1741 aatgattcta cagcactgaa atctagactt tcaatatctt cagctaaaag tttcccaagc
     1801 atacctacgg aatggatacc taaagctaat acacctgaaa atgaacctgg gccaacagct
     1861 ttgataaata taagtgccat tacaatttct gggaagacac gtataacact taaaataaat
     1921 ttgctaacac ctgaaactgg gcgtagcttt accatattat ttgcacctag aaatgctaat
     1981 ggaatacaga taattgcggc gatgaaagta cctacaacgg ctatcgcaaa ggtttcaagt
     2041 aaaccacgta ataagtcttc gccatctggt atatagatat agctgatatc aggatggaat
     2101 aatccgctga atatggattt taagatttct aatgatttac ttttaagttc taaacttggt
     2161 acacctgcaa atgcccagat gataatagct aagacgacaa ttgcaataag ccatctttta
     2221 atcaattttc gtttgtgtgc ttttgtgtga acattatatt tttctatttc ctgtgtcatg
     2281 cgagatgtgc cctcactttc gtactgatgt aatcaatgac gacgacgata actaaagtaa
     2341 ataaaataat cgttgctgtt tttggatatt gaaataaacc aagtgtttga tcataaaaca
     2401 atccaatacc gccagcgccg actaatccaa gcacagctga agcacgtata tttacttcaa
     2461 atgcatataa tacgtatgac ataaatgacg atatggcttg tggtacaaca ccgaaaacaa
     2521 tccattttat tttattagcg ccaacagccg tcattgcttc cattggacct ggatctatcg
     2581 tttccaatga ttcatataat aattttccaa taatacagat agttaaaata aacaatgcta
     2641 atatccctgg aatttgaccg attccaaata cagccacaaa gattgctgct aataacaaat
     2701 ctggaatagt acgaactata tttaaaataa agcgcgaggg tattgaaatc cacttttgat
     2761 gaacgatatt gctagcacat aataacgcaa ttggtattga aacgatgcta cctaatactg
     2821 tacttacgat agccattcga atggtatcta acattggcgt tgtaatttgt tgtaaatact
     2881 cgaaatcagg tggaatcatt tgtttgaata gatcacctat ttgaggtatt cctatcatta
     2941 aatctccaaa attaaacccc gtataaatga agctccaaat gataagcaca atgattaaca
     3001 tgaaggtaaa actcgttttt aaagaaacct ttttctttaa aagggagtca tactttgtag
     3061 gtgtttctaa aggcatgtta gttcactcct agcttttcat cttctttaat tgtacgtcca
     3121 tatattttac taaatacgtc atctgttgct tcagatgcag gaccatcata gacaacttca
     3181 ccatcacgta aaccaatgat gcgtgtgcca tattcttttg ctaagtcaac aaaatgtaaa
     3241 ttaattaaaa ttgtgatgcc taattcttgg ttgatttttc ttaaatcatc cataacctgt
     3301 ttcgttgtta atgggtctaa tgaagcaact ggttcatctg caagaataat ttcggattct
     3361 tggcatagcg cacgtgcaat agatatacgt tgttgttggc cacctgataa ttcatcagag
     3421 cgttgattat atttatctaa gatgttgacc cgttctagtg catccattgc tttaattttg
     3481 tcttcttttg gaaataaacc taataccatt ttccaagtag ggtgataacc tacacgtcca
     3541 cttagtacat ttcgtaatac acttgaccgt ttaactaaat taaaatgttg gaaaatcatg
     3601 cctatatttc ggcgcatttc taataatgct ttaccatggg ctttagtgat tgatttacct
     3661 tgaatgaaaa tttcacctga cgtgatatca tgcaaacgat ttacagatct taataacgtg
     3721 gatttcccag caccagatag tccgacaatg actgcaaatt cacctttttc aatatttaag
     3781 ttaatatttt tcaagcctac atgaccgtta ggatagactt tactgacgtt tttaaatttg
     3841 atttgactca tgtttgacac ctttctttaa taaagaaaag ccaaggtaaa ctgaaatatc
     3901 atagacattg ggtttctgtg tacaaatagt ttcaaatgta cagcgacacc tttctatatc
     3961 tgtttcagtt tagcgcctag cttttcaata ttaatctaga atatatctat tgaacgaaag
     4021 cttttaatac caaattcgat aatgattcat tggttaaata atgattattt catatcttta
     4081 actaattttt cgtactctct tacaatgtcg aaatttgaat ctttcgtttc tgtgtatcct
     4141 tcatgtgaat aaacttcgct aataattttg tgaccttctt ttgatttagc aatgtctata
     4201 aaagcttttt tcaatttctc ttggaagtct ttatccatat ctggtcttac agaaattgtg
     4261 tcattcggaa tagcttgtgt taattttaaa attcgtgtgt ctttaaatac atttggttgg
     4321 tcttttttca cagtattacg tgcatcgtta aatacagctg cagcatctac atctccattt
     4381 aataatgaga taactgcttg gtcatgacct ttaacattca caattttcat atctttagtt
     4441 gcattaatac ctgcttcgtt ttttaacatc gcaagtggga atgtatatcc agcagttgat
     4501 gttacatctt gtaaggcaat tttcttacct tttaaatctt tcaagctttt aatttttgag
     4561 tcttttttaa caagaatttc tgatttataa ctatctacaa gttctttact tgctgaacca
     4621 tcttctttta caccaaaacg ttgtgcttgt aataataaat cagctgcttt ttgatcatgt
     4681 gctaatgtgt atgccgttgg tggtaagaaa ccaacatcaa cttttttaga cttcatagct
     4741 tcaacaattg tattgtagtt agttgataca gacactttaa ctggaatccc taattcttta
     4801 gatagtaatt tttctaatgg ttttgcttta gcctctaatg ttccagcatt ttgcgaaggt
     4861 acaaattgaa ctgttaattc tttaggtttg tatcctcctg atttagaatc cgaatcatta
     4921 ctagcgttct tttgattatc taaagaactt gaatttccac atgctgctgc aaaaacaatg
     4981 actgctaaca ttaatacaaa taaacactta aaatttttca tttgataact gtccccttca
     5041 cattcttaat cgtgtaatat gtatgcaatg aaattacact tatatcgtac cacatttaaa
     5101 tgctaaaaag tattttataa aaatgaattt taataacttt ctttaagagt gtgataagat
     5161 tttgtaaaat tttcgttaaa ttgcccattt caaacatata tttatcctat aattcaagaa
     5221 caaatttatt ttaaaaggag cctcaaaaaa tgaaaaatat atataagtca ttaactgtct
     5281 ctgcaattgt tgcaacggta tcattaagtg ctttaccgca atctttagct ataacgcatg
     5341 aatcgcaacc tacaaagcaa cagcaaacag tattattcga tcgttctcat ggtcaaacag
     5401 ctggtgctgc agattgggtt agtgatggtg cattttcaga ttatgcggat tcaatacaaa
     5461 aacaaggtta tgacgttaaa gctattgatg gtcattcgaa cataacagaa gcaagtttga
     5521 aaagttccaa aatatttgta attcctgagg ctaacattcc tttcaaagaa tcagaacagg
     5581 cagcaattgt taactatgtg aaacaagggg gaaatgttgt ctttatttca gaccattaca
     5641 atgctgaccg aaatttaaat cgtattgatt catcagaggc aatgaatggt tatcgacgtg
     5701 gagcgtatga agatatgtcg aaaggtatga atgcagaaga aaaaagttct actgcaatgc
     5761 aaggtgtgaa aagttcagat tggttatcta caaactttgg cgtacgtttt cgatataatg
     5821 cactaggtga tttaaatacg agcaatattg tttcttcaaa agaaagtttt ggtattactg
     5881 aaggtgtgaa atctgtatct atgcatgccg gttcgacatt agcaattact aatccagaga
     5941 aagcaaaagg tattgtgtat acaccagaac aattgccagc gaaaagtaaa tggtcacatg
     6001 ctgtagatca aggtatttat aatgggggcg gtaaagcaga aggtccctat gtagcaattt
     6061 ctaaagttgg aaaaggtaag gcagcattta tcggtgattc atcacttgtg gaagatagtt
     6121 cgcccaaata tgtgagagaa gataatggag aaaagaagaa aacatatgat ggttttaaag
     6181 aacaagacaa cggtaagcta ttaaataata taacagcttg gatgtctaaa gataatgatg
     6241 ggaaatcact taaggcgagt ggcctaacat tagatacaaa gactaagttg cttgattttg
     6301 aacgaccaga gcgttcaact gagcctgaaa aagagccatg gtcacaaccg ccgagtggtt
     6361 ataaatggta tgacccaaca acatttaaag caggtagtta tggcagtgaa aaaggcgcgg
     6421 atcctcagcc aaacacacca gatgatcata cgccaccaaa tcagaccgaa aaagtatcat
     6481 ttgatatccc gcaaaatgtt tctgtaaatg agccatttga agtgacaata catttaaaag
     6541 gatttgaagc aaatcaaaca cttgaaaatc ttagagttgg tatttacaaa gaaggaggac
     6601 gtcaaatcgg acaattttca agtaaagata acgattataa cccgccaggt tacagtactt
     6661 tgccaacagt taaagcagat gaaaacggaa atgccacaat taaggtcaat gccaaagtac
     6721 tcgaaagtat ggaaggttca aagattcgtt taaaactcgg tgacaaaacc ttgattacaa
     6781 cagacttcaa ataaatatat aatagaaata agaaagatgt ttgtgattga aggagtgagt
     6841 gaggatgtca aacatagcat tttatgtcgt gagtgacgta catggttata ttttcccaac
     6901 agattttacg agtagaaatc aatatcagcc tatgggattg ttactagcga atcatgttat
     6961 agaacaagac agaaggcagt atgaccaaag ttttaaaata gataatggtg attttttgca
     7021 agggtcacca ttttgtaatt acttaatcgc gcatagcggc agtagccagc ctttagttga
     7081 tttttataat cgaatggcat tcgactttgg tacgcttggt aatcatgaat ttaattatgg
     7141 attaccatac ttaaaagaca ctttacgcag actcaattat ccagttttgt gcgctaatat
     7201 ttatgaaaat gatagtacat tgactgataa cggtgtgcag tattttcagg ttggagatca
     7261 aacggttggt gtgataggtt taacgacaca atttattccc cattgggaac aaccagagca
     7321 tattcagtca cttacgtttc atagtgcttt tgcaacactt caacaacact tacctgaaat
     7381 gaagcgatat gcagatatca ttgtggtttg ttaccatggt ggatttgaaa aggatataga
     7441 aagtggtacg ccgaccgaag tgttaacggg tgaaaatgaa ggatatggca tgttagaagt
     7501 gttttctaaa gatatagata tctttattac gggtcaccaa catcgacaaa ttgctgaaag
     7561 gtttaagcaa acggctgtga ttcaacctgg tacgagaggt acaactgtag gcaaagttgt
     7621 cttgagtact gatgaatatg aaaatgtatc cgttgaatca tgtgaattac ttcctgttat
     7681 agatgatccc acatttacta ttgatgaaga tgaccaacat atacgaaagc agctagagga
     7741 ctggttagat tacgaaatta caacattgcc atatgatatg atgattaatc atgcatttga
     7801 ggcacgtgta gctccgcatc cttttactaa ttttatgaat tacgctttat tagaaaaaag
     7861 tggagcagat gttgcctgta cagctttgtt tgattctgct agtggtttca agcaagtcgt
     7921 gacgatgcga gatgttatta acaattaccc atttccaaat acatttaaag ttttagctgt
     7981 aagtggtttc aaacttaaac aagccattga acgatcagca gaatattttg acgtgaaaaa
     8041 tgatgaagta agtgtgagcg cagacttcct tgaacccaaa ccccaacact ttaattatga
     8101 tatatatggt ggcgtaagtt ataccattca tgttggaaga ccaaagggac aacgtgtgag
     8161 caatatgatg attcaaggtc acgcagttga tttaaagcag acatatacaa tttgtgtaaa
     8221 taattatcgt gcagttggcg gtggtcagta tgatatgtat atcgacgcgc cagttgtaaa
     8281 agatattcaa gttgaaggcg cacaattact tattgatttt ttatcaaata ataaattgat
     8341 gcgcatcccg caagttgttg attttaaagt tgaaaagtga cggatatatg taaaacttac
     8401 ttgattttaa tcgggtaagt ttttgttttt gtttaaacta atcactctta aacaccaata
     8461 tactttaaga aaaatgttga ttttacaaaa aacaaggctt atattttata taacttaata
     8521 ttatgaagtg attaatttgg aaacaattaa acaagaagta agagaatcta ttgttaaagg
     8581 aattttcgcg ggatataagg actatgttaa tgagagaaat gaagtcaaga aacgtatggt
     8641 gattagtgat gcctatgtgt acaccaaatc taatcatatt gaaaatcaag ttgctaagca
     8701 tttggaaaat tttgttaagt atattaaaga aaatgctgga ccatcatgga agtatttaaa
     8761 gtttatattt aataaggatg aagagaaaaa taatatcatg tttatattga aaaatgaaga
     8821 ttactttgat gaaaaaaatg taagcgtagg gaagagttta gttgctgata agaatcaaaa
     8881 aagtaaaaac tacctagagg gacttatagc taagaatagt ggcattaatt ttgatactgt
     8941 tggagaagat tttaaaatta ggcatcaaat gactgcagat agtatattat ttaatataaa
     9001 agaaggcagt aatagagata ttaattctta ttttttaatt attacatatc gtattgataa
     9061 agaatcaaag caattagctg caatcaaaca atggttgcca aatccagaaa caaattctgc
     9121 aattatggtt gatgatttga caccattgtt agacaaagta atagtagaaa gagaagatta
     9181 tcatattgac gaagaagagt tagaagtact taaaaacgat ggagagttag aattcataga
     9241 tgtagaacat gcatttggta tcagtatcga tgaagataac gatatgattg aaagcgaaag
     9301 gtgaagttaa tgtttattgg caagaattta gaatacgtga gaaagcttaa tgctctaagt
     9361 agaaaagaat tatcagaaaa aataaatgta agtgaacaag caatttggca atatgaaaca
     9421 aaaaatatga tgccagaaat taataaaatt tatgatatga catctatttt taatgttaaa
     9481 tctagctact ttattagcga gcagcccgaa gagctcctaa taaattctgt cgataaacat
     9541 agtatagctt ttagagcaaa aaattataaa gtatctacga aactattaaa taagcaatat
     9601 tatcaagcaa tgtatttatc taatttaact agctacttat tttcctttgt aaagataccg
     9661 gataatatta ttttatcttt aataaataat ttagatgact tgttgaatgg caatttagaa
     9721 tcactaaata aaaaagaaag cataaaagaa actgctaaag ttgtgagggc taaaattctt
     9781 caagacgaaa gcaatgaagc actattattt atgcttgaaa aagcgggtat agttatctac
     9841 gaaaagcgaa ttaatgatag tatagacgca tatagttttt ggagtaaaga tcagacacct
     9901 tttattatac tgggtacaaa taagggggta gcagttagaa ggaattttga tcttgcacat
     9961 gagctgggac atctcgttct acatcgtcat attcaattcg atttattatc tcctgaagaa
    10021 tataagacaa ttgaacatga ggcagatatt ttcgcctctg aatttttact acctgaagaa
    10081 gcgtttaaaa aagactttga tcaaatgaca aaaaaatcaa atcctgatta tttagctgta
    10141 ttgaaagaaa aatggtatgt ttctatacaa gcaattgcaa tgagagccta ttatctaggt
    10201 ctaatgtcta gtactcagta cagatatttt tgggcatcat taaataaaaa aggctataaa
    10261 agtaaagagc ctttagacga cgtgattgaa atgtcacgtc ctgtcaaaat gaatagttta
    10321 ttaaagttgt attttgatag aaatatactg acaccacaaa aattattgaa ttatttaaaa
    10381 gttgatgaaa catttttgaa tcatctagca ggtattaatt taaaactctt taaggattat
    10441 gttaatgaaa atagagaata taatataacg aatctatata aataagacac caatatttaa
    10501 acctcgagct gaaaagatta tcatttcagt tcgaggtttt tatttgcaac atctgttgat
    10561 aggtatcgac aaggaccttt taatgttgtg ttttgtgcga tataacaagc tttttaggct
    10621 attaaagaat attcagaatt aaatatataa ttatgaataa attatgtcat tgaaaaaatt
    10681 atgattataa aatcattcta aaaacaccga aataacaatg atttcatgaa aacatttatt
    10741 ttaaaatttg atatttgttc aaataatatt cgaaattaaa cttttttgta tagaattttc
    10801 tttatatcct gagagacatg tactataatg tttgtgaaat aattcacaaa gtataaagga
    10861 gtggttgtat atgttaacta tacctgaaaa agaaaatcgt ggatcgaaag aacaagaagt
    10921 ggcaattatg attgatgctc tagctgacaa agggaaaaag gcattagaag cattatctaa
    10981 aaagtcacaa gaagaaatta atcatattgt tcatcaaatg agcttagcag ctgttgatca
    11041 acatatggtg ctagcaaaat tagcacatga agaaactgga agaggtatat acgaagataa
    11101 agcgattaaa aatttatacg cttctgaata tatatggaat tcaataaaag acaataagac
    11161 agtagggatt attggtgaag ataaagaaaa aggattaacg tatgtagcgg aaccaattgg
    11221 tgttatttgt ggtgttacgc caacaacaaa tcctacgtcg acaactattt ttaaagcgat
    11281 gattgcaatt aagacaggaa atccaatcat ttttgcattc catccaagtg cgcaagaatc
    11341 gtcgaagcgt gcagcagaag ttgtattaga agcggcaatg aaggcaggtg cacctaaaga
    11401 tattattcaa tggattgaag tgccctctat cgaagcaaca aaacaattaa tgaatcacaa
    11461 aggtattgca ttagttctag caacaggtgg ttcgggcatg gttaagtctg catattcaac
    11521 tggcaaaccg gcattaggtg tgggaccagg taacgtgccg tcttacattg aaaaaacagc
    11581 acacattaag cgtgcagtaa atgatatcat tggttcaaaa acatttgata atggtatgat
    11641 ttgtgcttct gaacaagttg tagtcattga taaagaaatt tataaagatg ttactaatga
    11701 atttaaagca catcaagcat actttgttaa aaaagatgaa ttacaacgct tagaaaatgc
    11761 aattatgaat gaacaaaaaa caagtattaa acctgatatt gtcggtaaat ctgcagttga
    11821 aatagctgaa ttagcaggta tacctgtccc cgaaaataca aaacttatca tagccgaaat
    11881 tagcggtgta ggttcagact atccgttatc tcgtgaaaaa ttatctccag tattagcctt
    11941 agtaaaagcc caatctacaa aacaagcatt tcaaatttgt gaagacacac tacattttgg
    12001 tggattagga cacacagccg ttatccatac agaagatgaa acattacaaa aagattttgg
    12061 actaagaatg aaagcttgtc gtgtacttgt aaatacacca tcagcggttg gaggtattgg
    12121 tgatatgtat aacgaattga ttccgtcttt aacattaggt tgtggttcgt acggtagaaa
    12181 ctcaatttca cataatgtta gtgcgacaga tttattaaac attaaaacaa ttgctaaacg
    12241 acgtaataat actcaaattt tcaaggtgcc tgctcaaatt tattttgaag aaaatgcaat
    12301 catgagtcta acaacaatgg acaagattga aaaagtgatg attgtctgtg accctggtat
    12361 ggtcgaattc ggttatacaa aaacagttga gaatgtatta agacaaagaa cggaacagcc
    12421 tcaaattaaa atatttagcg aagtcgaacc gaacccatca acgaatacag tatataaagg
    12481 tctggaaatg atggttgatt tccaaccgga tacaatcatt gcacttggtg gtggttcagc
    12541 gatggatgct gcaaaagcaa tgtggatgtt ctttgaacac cctgagacat cattcttcgg
    12601 tgctaaacaa aagttcctag acatcggtaa acgtacttat aaaataggca tgcctgaaaa
    12661 tgcgacgttc atttgtatcc ctacgacatc aggtacaggt tcagaagtaa caccatttgc
    12721 agttatcaca gatagtgaaa caaatgtaaa atatccgttg gctgattttg ctttaacacc
    12781 tgatgttgca attattgacc ctcaatttgt gatgagtgtg ccaaaaagcg ttacagcaga
    12841 tacaggaatg gatgtactaa cgcatgcaat ggaatcatat gtatctgtaa tggcttcaga
    12901 ttacacaaga ggtttgagtc tacaagcgat taaattgacg ttcgaatatt taaaatcatc
    12961 tgttgaaaaa ggtgataaag tttcaagaga gaaaatgcat aacgcatcaa ctttggctgg
    13021 tatggcattt gcaaatgcat tcttaggcat tgcacactca attgcgcata aaattggtgg
    13081 ggaatatggt attccgcatg gtagagcgaa tgcgatatta ctaccgcata ttatccgtta
    13141 taatgccaaa gacccgcaaa aacatgcatt attccctaaa tatgagttct tcagagcaga
    13201 tacagattat gcagatattg ccaaattctt aggattaaaa ggtaatacga cagaagcact
    13261 cgtagaatca ttagctaaag ctgtctacga attaggtcaa tcagtcggaa ttgaaatgaa
    13321 tttgaaatca caaggtgtgt ctgaagaaga attaaatgag tcaattgata gaatggcaga
    13381 gctcgcattt gaagatcagt gtacaactgc taatcctaaa gaagcgctaa tcagtgaaat
    13441 caaagatatc attcaaacat catatgatta taagcaataa tctatctgat aataatcatc
    13501 taactcacct gaaattacaa aagtaaaaaa tgccacataa acttaaagtc gataatcatt
    13561 atacggttat cggcttttct ttattgccaa atcttcagag agatacaacc tagacaatca
    13621 atttttttaa taaagaaagt attaagattg gtacactttt cacaaactat tactacttta
    13681 gagtataatt atttttaatt tcatataaat aaaaaggcga aaataatgcg gtttaaaagt
    13741 aattaattgt ttaaacgata tgtaatatgt aaacactata tatataatac caattttaat
    13801 gaaaattttt aagggaggta aataatggaa agtacattag aattaacaaa aattaaagaa
    13861 gtattacaaa aaaacttgaa gattttaatt attttaccgc tattattttt aattattagc
    13921 gctattgtta catttttcgt cttatcacct aaatatcaag ctaatactca aattttagtg
    13981 aatcaaacta agggtgacaa tcctcagttt atggcgcaag aggttcaaag taatattcaa
    14041 cttgtaaata cgtataaaga aattgttaaa agtcctagaa ttttagatga ggtgtcaaag
    14101 gacttaaatg ataagtattc accatctaaa ttatcgagta tgttgacaat tacaaaccaa
    14161 gaaaatacac aacttatcaa catccaagtt aaaagtggtc ataaacaaga ttcggaaaaa
    14221 attgcgaata gcttcgctaa agttacaagt aaacaaattc cgaagattat gagtgtggat
    14281 aacgtatcaa ttttatctaa agcagacggt acagcagtta aagtcgcacc aaaaactgta
    14341 gtgaatctaa tcggtgcact ctttttagga ttagttgtcg cgcttatata tatcttcttc
    14401 aaagtaattt ttgataagcg aattaaagat gaagaagatg tagagaaaga attaggattg
    14461 cctgtattgg gttcaattca aaaatttaat taaggatggt tgctacttat gtcaaaaaag
    14521 gaaaatacga caacaacact atttgtgtat gaaaaaccaa aatcaacaat tagtgaaaag
    14581 tttcgaggta tacgttcaaa catcatgttt tcaaaagcaa atggtgaagt aaagcgctta
    14641 ttggttactt ctgaaaagcc tggtgcaggt aaaagtacag tcgtatccaa tgtagcgatt
    14701 acttatgcac aagcaggtta taaatcgtta attatcgatg gggatatgcg taagccaaca
    14761 caaaactata tttttaatga acaaaataat aatggattat cgaatttaat cattggtaga
    14821 acgactatgt cagaagctat tacgacgaca gaaattgata atttagattt actaacagct
    14881 ggtcctgtac ctcctaatcc aacagaatta attggttctg aaagatttaa agagttagtt
    14941 aatcttttca atgagcgtta tgacatcatt attgtcgata caccgccagt taataccgtg
    15001 actgatgcac aactatatgc acgtgctatt aaagatagtc tgttagtcat tgatagtgaa
    15061 aaaaatgata aaaatgaagt taaaaaagct aaaacactta tggaaaaagc aggcagtaat
    15121 attctaggcg tcattttaaa taaggcaaaa gtagataagt cttctagtta ttatcactat
    15181 tatggagatg aataggtatg attgatattc ataaccatat attgccgaat atcgatgacg
    15241 gtccgacaaa tgaaacagag atgctggatc ttttaagaca agcgacaaca caaggtgtta
    15301 cagaagtcat tgtaacatca catcacttac atcctcgata taccacacct atagaaaaag
    15361 tgaaatcatg tttaaaccat attgaaagct tagaggaagt acaagcacta aatctaaagt
    15421 tttattatgg tcaggaaata agaattaccg atcaaatcct taatgatatt gatcgaaaag
    15481 ttattaccgg tattaatgat tcacgctatt tactaataga atttccatca aatgaagttc
    15541 cacactatac tgatcaatta tttttcgaat tacagagtaa aggctttgta ccgattattg
    15601 cacatccaga gcggaataaa gcaataagtc aaaaccttga catactatac gatttaatta
    15661 acaaaggtgc tttaagtcaa gtgacaacgg cgtcattagc gggtatttcc ggtaaaaaaa
    15721 ttagaaaatt agcaattcaa atgattgaaa acaatctgac acatttcatc ggttcagatg
    15781 cgcataacac agaaatcaga ccgttcttaa tgaaagactt atttaatgat aagaaattac
    15841 gtgattatta tgaagatatg aacggattta ttagtaatgc gaagttagtt gttgatgata
    15901 aaaaaattcc taaacgaatg ccacaacaag attataaaca gaaaagatgg tttgggttat
    15961 aaacagcaaa tgaggggttt tatggcacat ttatctgtga aattgcggct tttaatacta
    16021 gcattaatcg attcactgat agtgacattt tcagtattcg taagttatta cattttagaa
    16081 ccgtatttca aaacatattc tgtcaaatta ttaatattgg cagctatatc actattcata
    16141 tcgcatcata tttcagcatt tatttttaat atgtatcatc gagcgtggga atatgccagt
    16201 gtgagtgaat tgattttaat tgttaaagct gtgacgacat ctatcgttat tacgatggtg
    16261 ttcgtgacaa ttgttacagg caatagaccg ttttttaggt tgtatttaat tacatggatg
    16321 atgcacttga ttttaatagg tggctcaagg ttattttggc gtatttatcg gaaatacctt
    16381 ggaggtaagt catttaataa gaagccaact ttagttgttg gtgctggcca agcaggttca
    16441 atgctgatta gacaaatgtt gaaaagtgac gaaatgaaac ttgaaccggt attagcagtc
    16501 gatgatgacg aacataaacg taatatcaca attactgagg gtgtaaaagt ccaaggtaaa
    16561 atcgcggata ttccagaact agtgaggaaa tataagatta aaaaaattat cattgcaatt
    16621 ccaactattg gtcaagagcg tttgaaagaa attaataata tttgccatat ggatggcgta
    16681 gagttattga aaatgccaaa tatagaagac gtcatgtctg gtgagttaga agtgaaccaa
    16741 ctgaaaaaag ttgaagtaga agatttactt ggcagagatc ctgttgaatt agatatggat
    16801 atgatatcaa atgaattgac gaataaaact attttagtta cgggcgctgg aggttcaata
    16861 ggatcagaaa tttgtagaca agtttgtaat ttctatccag aacgtattat tctacttggc
    16921 catggtgaaa acagtattta tttaatcaat cgtgaattgc gaaatcgctt cggaaaaaat
    16981 gttgatatcg ttcccattat agcggatgtg caaaatagag cgcgtatgtt tgaaattatg
    17041 gaaatgtata aaccatacgc agtttatcat gcagcagcac acaagcacgt gccgttaatg
    17101 gaagacaacc ctgaagaagc agtacgtaat aatattttag gtacgaaaaa tactgctgaa
    17161 gctgctaaaa atgcagaggt aaagaaattc gttatgattt ctacggataa agccgttaat
    17221 ccacctaatg tcatgggagc ttcaaagcga attgcagaaa tgattattca aagtttaaat
    17281 gatgaaacgc atcgaacaaa ttttgttgca gtgagatttg gtaatgtact tggatcgaga
    17341 ggatctgtga ttccactttt caaaagtcaa attgaagaag gtgggccagt tactgtgaca
    17401 catcctgaaa tgacacgtta ctttatgaca attcctgaag cttctagact agttttgcag
    17461 gcaggggcat tagcagaagg tggcgaagta tttgtgttag atatgggaga accagtgaaa
    17521 atagtagatt tagcacgaaa tttaattaag ttaagtggta agaaagaaga cgatatacgc
    17581 attactttta cagggattag acctggtgaa aaaatgtatg aagaattgat gaataaggat
    17641 gaaattcacc ctgaacaagt atttgaaaaa atttatcgtg gcaaagtaca acatatgaaa
    17701 tgtaatgaag ttgaagcgat tattcaagac atcgtcaatg actttagtaa agaaaaaatt
    17761 attaactatg ccaatggcaa aaagggagat aattatgttc gatgacaaaa ttttattaat
    17821 tactgggggc acaggatcat tcggtaatgc tgttatgaaa cggtttttag attctaatat
    17881 taaagaaatt cgtatttttt cacgcgatga gaaaaaacaa gatgacattc gaaaaaaata
    17941 taataattca aaattaaagt tctacattgg tgatgtgcgt gatagtcaaa gtgtagaaac
    18001 agcaatgcga gatgttgatt acgtattcca tgcagcagct ttaaaacaag tgccgtcatg
    18061 tgaattcttt ccagttgagg cagtgaagac aaatattatt ggtacagaaa atgtcttaca
    18121 aagtgctatt catcaaaatg ttaaaaaagt catatgttta tctacagata aggcagcgta
    18181 tcctattaat gctatgggta tttcgaaagc aatgatggaa aaagtattcg tagctaaatc
    18241 aagaaatatt cgtagtgaac aaacgcttat ttgcggtaca agatacggta atgtaatggc
    18301 ttcaagaggt tcagtaattc ctttgtttat cgataaaatc aaagctggag aacctttaac
    18361 gattacagat cctgatatga caagattttt aatgagctta gaagatgcgg tagaactagt
    18421 tgttcatgca tttaagcatg cagagacagg cgatattatg gttcaaaaag caccaagttc
    18481 aacggtaggg gatcttgcga ccgcattatt agaattgttt gaagctgata atgcaattga
    18541 aatcattggt acgcgacatg gagagaaaaa agcagaaaca ttgttgacga gagaagaata
    18601 cgcacaatgt gaagatatgg gtgattattt tagagtgccg gcagactcca gagatttaaa
    18661 ttatagtaat tatgttgaaa ccggtaacga aaagattacg caatcttatg aatataactc
    18721 cgataataca catattttaa cggtggaaga gataaaagaa aaacttttaa cactagaata
    18781 tgttagaaac gaattgaatg attataaagc ttcaatgaga taggagagat tgacgttgaa
    18841 tattgtaatt acaggagcaa agggttttgt aggaaaaaac ttgaaagcag atttaacatc
    18901 aacgacagat catcatattt tcgaagtaca tcgacaaact aaagaggaag aattagagtc
    18961 agcgttgttg aaagcagact ttgtcgtgca tttagcggga gttaatcgac ctgaacatga
    19021 caaagaattc agcttaggaa acgtgagtta tttagatcat gtacttgata tattaactag
    19081 aaatacgaaa aagccagcga tattattatc gtcttcaata caagcaacac aagataatcc
    19141 ttatggtgag agtaagttgc aaggtgaaca gctattaaga gagtatgccg aagagtatgg
    19201 caatacggtt tatatttatc gatggccaaa tttattcggt aagtggtgta agccgaatta
    19261 taactctgtg atagcaacat tttgttacaa aattgcacgt aacgaagaga ttcaagttaa
    19321 taatcggaat gttgaactaa cgctaaacta cgtggatgat atcgtcgctg aaataaaacg
    19381 tgctattgaa ggaaatccaa cgattgaaaa tggtgtacct acagtaccaa atgtatttaa
    19441 agtgacattg ggagaaattg tagatttatt atacaagttc aaacagtcac gtctcgatcg
    19501 aacattgccg aaattagata acttgtttga aaaagatttg tatagtacgt atttaagcta
    19561 tctacctagt acagacttta gttatccctt acttatgaat gtggatgata ggggatcttt
    19621 tacagaattt ataaaaacac cggatcgtgg tcaagtttct gtaaatattt ctaaaccagg
    19681 aattactaaa ggtaatcact ggcatcatac taaaaacgaa aaatttctag tcgtatcagg
    19741 taaaggggta attcgtttta gacatgttaa tgataatgaa atcattgaat attacgtttc
    19801 tggcgacaaa ttagaagttg tagacatacc agtaggatac acacataata ttgaaaattt
    19861 aggcgacaca gatatggtaa ctattatgtg ggtgaatgaa atgtttgatc caaatcagcc
    19921 agatacgtat ttcttggagg tatagcgcat ggaaaaacta aaattaatga caatagttgg
    19981 tacaaggcct gaaatcattc gtttatcatc aacgattaaa gcatgtgatc aatattttaa
    20041 tcagatatta gtacacactg gtcaaaatta tgattataca ttgaatcaaa ttttctttga
    20101 tgatttggaa ttaagacaac cggaccacta cttagaggca gttggaagta acctcggaga
    20161 aacgatgggg aatattattg cgaagacata tgacgtttta ttacgcgaac aaccagatgc
    20221 acttttaatt cttggtgata caaatagttg tttagcagca gtatctgcta aacgattaaa
    20281 aattcctgtg ttccatatgg aagcgggtaa tagatgcttt gatcagaatg tacctgaaga
    20341 aatcaatcgt aaaattgttg accatgtcag tgatgtgaat ctaccttata cagaacatag
    20401 cagacggtat ttattagatg aaggcttcaa taaagcgaat atctttgtga caggatcacc
    20461 gatgacagaa gtgatagaag cgcatcgaga taaaattaat cacagtgacg ttttaaataa
    20521 actaggatta gaaccgcaac aatacatttt agtatctgcg catagagaag agaatatcga
    20581 taatgaaaag aattttaaat cattaatgaa tgcgataaat gatattgcca aaaagtataa
    20641 aattcctgtg atttattcaa cgcatccaag aagttggaag aaaattgaag aaagtaaatt
    20701 tgaatttgat ccattagtta aacagttaaa gccatttggt ttctttgatt ataatgcatt
    20761 gcaaaaagat gcatttgttg tgctatcaga tagtggaaca ttgtcagaag agtcgtctat
    20821 tttgaagttc cctggtgtcc ttattcgaac ttccacagaa agaccggaag tactagataa
    20881 aggtacggtt attataggtg gtattaccta taacaatcta atccaatccg ttgaactagc
    20941 aagagagatg caaaacaata acgaaccgat gattgatgct attgattata aagacactaa
    21001 cgtttcgaca aaagtagtta aaattattca aagctataaa gatattatca atcgaaatac
    21061 ttggaggaaa tgacgatgag gatagcgatt cttggcgcta ctaacattaa gcatatgtca
    21121 ttattatcac attatttaaa ccacattgat ttgaatatca atgaggtgga cattatatac
    21181 actgacaaat atgatatcga agaaaatatc caaggcatca ataattacta taaatataaa
    21241 gtagatatta aagaagattg gacatttatc aaaaaagcta ttgcttacta tcgatttagg
    21301 ccatacgcta tgaaaattct caaagaaaat cattatgatt ttgtcatagt atggggaagt
    21361 tatacaggac acttatttaa aagtttttta gaaaaacact ataaaaataa attcatttta
    21421 aatataaggg actacttttt tgaaaataat aaacttatta agtatagaat gaaaaaaatc
    21481 gttgatgcta gcaggctgac aacattatct tcagaaggtt ttcttaaatt tttacctaaa
    21541 tctgaaaaat atagaattat ttatagttat aacatgagta ttattagaga aagtaatgtg
    21601 accgatgaat tcaaaaaaag atggccaatt aatataggtt ttattggtaa tgtcagattt
    21661 aatgagataa atcaaaaatt gataaaggaa ctggcaaatg attcaaggtt ccatatgcaa
    21721 tattttggaa cagggtcgga aaaattagaa gtctttgctc gagaaaattt tattaataat
    21781 attacatttt ctggcggttt tgacttggaa gaaacaccga aatatttaaa tgaaattgat
    21841 atacttaata atttgtttgg taataaaaat attgctttag atactgcttt atccataaga
    21901 atgtattatg cgttgttttt aaacaaacct attattacaa cagatgatac attcaccgct
    21961 acagaagcta ataaattcgg actgggtttt agtgttaatc cagaaaattt aaaaggtatt
    22021 ggtgatgaat tgatggattg gtataacaat ttgaatgtaa tggacattaa tcataaaaga
    22081 gaagcttata gaaacgatgt aattgaaaat aataaacagt tctatcaaga gataggcagg
    22141 atatttaatg aataagatat ataatgtcac ttcatatgtt attgccattt taatgtttcc
    22201 ttgccttatg ttaggtgata aacctttatt gtttttagca cctataagtt atggagtagg
    22261 aaagctcttt ataagcttct caaataatcc gaattttaaa ttttcgaaaa ttgtatacga
    22321 tgttttaggt tttcttagat tagtatttat acctgctatg atagtgtttt tccaggattc
    22381 aactatagat aatttaccat taggacaaga ttattttaat caagcggtta tttatatgag
    22441 tgtggagttt atcataggct cgctatttat attgatacta tctaaattat tcaaacatga
    22501 agtggtatca agaaatagct ttacactttc tggatcatca atttattaca ttgtgtttgg
    22561 tcttgttatt tgtgggattt ttgtagcttt tcccgaagtg cgcaaaaaca tatcattttt
    22621 aattattaaa acagatgcaa tgggaagagg aaccgaagca acaagtggtt taaatgtcct
    22681 ttttgtaatg ctatttcaac ttgccttagc gttattattc ttaataatcg catatgcttc
    22741 atataaaaag tataaagaga atcctaaaat tatttatgtt atattgccgc tagctatagg
    22801 gattttaaat attagtttaa ttgttggtga aagaagaagt tatcaacttt atacgatggt
    22861 tgctgtttta acagtggtgt cactcttgtt ttctaagcac aaaagacgta tcaatatcat
    22921 tattatatct gttggtattt tcgtattagc attgatgaca ctatataaag aattgtatgt
    22981 gtttaattat agttcatata gcgaggcgtt aaatagtacg agtgtaagta atcttaagat
    23041 agttgacacg ttgcagtcat atttttatgg acctagcaat attgcagctt ctatagacta
    23101 tttgaattac tataatggtt cgtttaaaca atatttattt gataatacta gagctgtttt
    23161 tgggtttaat ttctttgtag ataaaaagca gttgattact agccaacttt ttaaccaatt
    23221 gatatatggt agtaaacaat tgactggtca tctaatttcg agtgctggat atggaattat
    23281 ttattttgga cccttattct tctatttgaa tttaattgca aatatctttt ttgcatttct
    23341 gagtgagtat attattcgga agagtcattc gttagaagtg atatttattg gtacatatat
    23401 ttatatgaga cttattacga gtatttttag tcacccgaca ccattaatca cattgatttc
    23461 tatgatttta gttgtatatg ttatagctat tatcccaggc attataataa aaaaatttac
    23521 taaaaaagta gggatagaat gattgtaaaa acatttatga aatcgaaaat atttagatta
    23581 atgaatacac cgctattatt attttataag aaagaatatt taactggata ttattttgaa
    23641 aataaagtgg ctggatggtt atgggcatgg aaagctgttc cgttcaagtt gttaggaata
    23701 aatacgagct tgccatttcc tgcagatata actgttagaa tgcataaccc taataacatt
    23761 gtttttgata aaaatgatat tcatattttc caatcgcccg ggacgtattt taataatttt
    23821 tcagcagtta tatatatagg tagaggtgtt tatatagcgc caaacgtagg tattattaca
    23881 gctaatcata atattaaaaa tttgaagtca catgcaccag gtgaagatgt caaaataggg
    23941 aattatagtt ggattggaat gaactcagtt atattaccag gagtagaatt gggggaacat
    24001 acaattgtag gggctgggtc ggttgtaaca aaaagttttc cagaaggtaa cgttgttata
    24061 ggtggcaatc cagcgaaagt catcaagaaa atctgaggtt aattatgaga ttaaataaat
    24121 ttattggcga ttcgttctta atgattttaa gcagtggcat cgctcaagtc atattaatca
    24181 tcactacccc aattattaca agactatatt cacctacaga atttggtgag tttacaattt
    24241 tttcaaatat cgcaatgatt ttaataccaa taataaatgc aagatacgat ttgttgattg
    24301 tgaatactaa aaatgaccgt agtgctaata tactttcaca aatcagtttt ttgatatcat
    24361 tgcttatttt attaatactg ataccaatat ttgcgattag tgcatggtta tacccaaact
    24421 ttatattaga ttttattttc attattatta tgttgttttt ggtaagttta acaaacattt
    24481 ttacaaatta tctaaataag gaaagaaaat ataaagtgtt aagtttgatt aatgtgttta
    24541 gagctggatc aatggcttta cttcaaatta ttttcggact tttagcatta ggaagtctag
    24601 gattaattat tggtttttca ttatcctata tcgcaggcat tacactagga tataaaacgt
    24661 ttaaaaagca ctttaatatt gtgagagata aagaagaaac taaagcatta tttttagaaa
    24721 ataaaaatca gttagtttat tcaacaccat caatattatt aaatagtttg tctttctcgg
    24781 ttgttgtgtt ctttataggt attttgtata ccaatacaga agtaggtatt tatggtatgg
    24841 ccataagagt actaggcata ccagtgacaa ttatttcatt agggttatca aaaatattta
    24901 tgcaacaagc taatgactat tatattgaat atggtaactt ccgaaattta ttacttaaat
    24961 ttagttccat actggttata gtttctataa ttctttatgt gccactttat ttgttcagtg
    25021 aagaattagt caatatatta ttaggacata gctgggttga cgcaattaca gttataaaaa
    25081 ttgttattcc attatttgtt ataaggctga ttgtatcaac ggtatcactt tctgtgattg
    25141 tattacaaaa acaacagtta gaattaatac tgcaagcgtt atttttaata ggtactactg
    25201 caacatttgt tatatcaaaa atgcttaatt taactttttt aaactttgta tctattaata
    25261 cagttgtttt aatcgtatcg tacatgatat ttttcatagc actctattat tttgctaaaa
    25321 ataaacagtt caaaaattct tagtctaaat tcattaataa aaaggacgtc gattagatga
    25381 gtgaaaaaaa gattttgatt ttatgtcagt atttttatcc ggaatatgta tcttcagcga
    25441 cgttaccaac acaattggcg gaagatttaa ttgcgaatca cattaatgtc gatgtcatgt
    25501 gtggatggcc atatgaatat agtaatcata aacaggtttc taaaaccgag atgcatcgtg
    25561 gtattcgcat tcgacgtctc aagtattcga ggtttaataa caaaagtaag gttggaagga
    25621 tcatcaattt ctttagttta ttttcaaaat tcgtgattaa tatacctaaa atgttgaaat
    25681 atgatcagat tcttgtttac tctaatccac caatcttgcc attaatacca gacgttctac
    25741 acagattgct taagaaaaaa tattcttttg tggtgtatga tatagcacct gataatgcga
    25801 ttaagacagg tgcaacacgt ccaggtagca tgattgataa actgatgcgt tacattaata
    25861 aacatgtcta caagaatgct gaaaatgtca ttgtccttgg tacggaaatg aaaaactact
    25921 tactaaatca tcaaatttct aaaaatcctg acaatattca tgtgattcct aactggtatg
    25981 acatgcgtca gttacaagac aatcgtatct ataatgacac atttaaagct taccgtgagc
    26041 aatacgacaa aattttattg tatagcggta atatggggca gttacaggat atggagacac
    26101 ttatctcatt tttaaaatta aataaggatc agcctcaaac gttaacaata ctttgtggtc
    26161 atggtaagaa atttgcagat gtcaaaacgg caatagaaga ccatcgtatt gaaaatgtta
    26221 aaatgtttga gtttttaaca ggtacagact atgctgacgt attaaaaatt gcggatgtat
    26281 gtattgcatc gctgattaaa gaaggcgtcg gtttaggcgt gccgagcaag aattatggct
    26341 accttgcagc taagaagccg ttggtactca tcatggataa gcaatctgat atcgttcaac
    26401 atgttgaaca atatgatgcg ggtatccaaa ttgataatgg cgatgcacat gccatttata
    26461 acttcatcaa cactcactcg agtaaggaat tgcacgagat gggtgagcgc gcacatcaac
    26521 tgtttaaaga taaatatacg agagaaatta atactatgaa gtattacaat ctgttgaagt
    26581 gaggagataa ttatgaagcg attattcgat gtagtgagtt caatatatgg tttagtagtt
    26641 ttaagtccga ttctgttaat tacagcatta ctaattaaaa tggaatcacc cggaccagcc
    26701 attttcaaac aaaaaagacc gacgattaat aatgaattgt ttaatattta taagtttaga
    26761 tcaatgaaaa tagacacacc taatgttgca actgatttaa tggattcaac agcgtatata
    26821 acaaagacag ggaaggtcat tcgtaa
//
LOCUS       DAMZPP010000017        26651 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMZPP010000017 DAMZPP010000000
VERSION     DAMZPP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.017"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..89)
                     /locus_tag="POH73_000551"
     CDS             complement(<1..89)
                     /locus_tag="POH73_000551"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="catalyzes the interconversion of galactose
                     6-phosphate to tagatose 6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDI6886529.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEV"
     gene            complement(105..533)
                     /gene="lacA"
                     /locus_tag="POH73_000552"
     CDS             complement(105..533)
                     /gene="lacA"
                     /locus_tag="POH73_000552"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDI6886530.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            complement(900..1691)
                     /locus_tag="POH73_000553"
     CDS             complement(900..1691)
                     /locus_tag="POH73_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6886531.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIARKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            1897..2637
                     /locus_tag="POH73_000554"
     CDS             1897..2637
                     /locus_tag="POH73_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDI6886532.1"
                     /translation="MFNLKHDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHSTLNRFYCNACHKSYMKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEDADTLVVLGSSLVVQPAAGLISNFKGDNLIIIN
                     KDRTPYDNDATLVIHDDMVSVVKSLMTE"
     gene            2674..2890
                     /locus_tag="POH73_000555"
                     /pseudo
     CDS             2674..2890
                     /locus_tag="POH73_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789088.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2895..3503)
                     /locus_tag="POH73_000556"
     CDS             complement(2895..3503)
                     /locus_tag="POH73_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HDI6886533.1"
                     /translation="MCFDINNFDIKKLNFRKVKNAIHLSLSGDKYQFLEDGKTINNTY
                     FLAVYDNAINIFEDLFSPSDSINLVHVVYVYNEPYKRTNVIKKFTSLTIDELYYFKEY
                     KKEKDDNILCEEFSYKCSIKDIKYKKLLKAISNQDFKGLVPNINQNDNYSEIYIINNT
                     KNLIYHLYDDRGLWLAFNNNEDYARYSEKYNDLILEFDNEDI"
     gene            complement(3517..3810)
                     /locus_tag="POH73_000557"
     CDS             complement(3517..3810)
                     /locus_tag="POH73_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886534.1"
                     /translation="MSENAIIHDDYFYNLKAVKTHNIAKNVNKSLLNDKGVSIGKFIQ
                     KLKGKNPTWRYPKIKWTISKNKGQSYGGSYWKLINNKGKRIASLTKEGKILRE"
     gene            complement(3765..3890)
                     /locus_tag="POH73_000558"
     CDS             complement(3765..3890)
                     /locus_tag="POH73_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886535.1"
                     /translation="MTYIPFSIITLITGIIMHMTMYFVKFECRKMPLFMTIIFTI"
     gene            complement(4111..4959)
                     /locus_tag="POH73_000559"
     CDS             complement(4111..4959)
                     /locus_tag="POH73_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6886536.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYIDL
                     VLIHQPYNDVYGSWRALEELKETGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            complement(5029..5445)
                     /locus_tag="POH73_000560"
     CDS             complement(5029..5445)
                     /locus_tag="POH73_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI6886537.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDNDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     ARDLLQYKIDNYDNHIAKINASDNHDDNVERLWERK"
     gene            5688..5855
                     /locus_tag="POH73_000561"
     CDS             5688..5855
                     /locus_tag="POH73_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886538.1"
                     /translation="MLKIWVIDCLYASTFFVDCLTVDSQLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
     gene            5852..8278
                     /locus_tag="POH73_000562"
     CDS             5852..8278
                     /locus_tag="POH73_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HDI6886539.1"
                     /translation="MTYRMKKWQKLSTITLLMAGVITLNGGEFRSIDKHQIAVADTNV
                     QTTDYEKLRNIWLDVNYGYDKYDENNPDMKKKFEATENEAEKLLKEMKTESDRKYLWE
                     SSKDLDTKSADMTRTYRNIEKISEAMKHKNTKLKTDENKTKVKDALEWLHKNAYGKEP
                     DKKVADLTSNFKNKTSRNTNLNWWDYEIGTPRALTNTLILLQEDFTDEETKKYTAPIK
                     TFAPDSDKILSSVGKSEPAKGGNLVDISKVKLLESIIEEDKDMMKKSIDSFNTVFTYA
                     QNSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDSNQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDTMDKSTK
                     AKYKKIVKTSVESDSSYKQTDYLSSYSDISKMKSLMEDSTISTNGLTQQLKIYNDMDR
                     VTYHNKGLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYRDNFWA
                     TADMKRLAGTTTLDNEEPKSTDVKKSSKTFVGGTKFDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANDYKLYKDDTQTTNSDNQETNSLF
                     LESTNSTQNNIGYHFLNESKITVKKESHTGKWSDINKSQKDIQKTDEYYEVTQKHSNT
                     DSKYAYVLYPGLSKDVFKSKASKVTVVKQEDDFHVVKDNESVWAGINYSDSAKTFEIN
                     NTKVEVKAKGMFILTKKDDNTYECSFYNPESTNSVSDIESKISMTGYSIINKNTSTSN
                     ESGVRFELTK"
     gene            complement(8338..9192)
                     /locus_tag="POH73_000563"
     CDS             complement(8338..9192)
                     /locus_tag="POH73_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDI6886540.1"
                     /translation="MNKTNFGDYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTW
                     QSDVHELKVCTQNDGTIEQIWIDKANQFGVAVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEVFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNLGKVNADYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTKESQFLGNYIVIKHAENEYSLIAHLQQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFINNRELIKGD
                     VVCGLQGE"
     gene            complement(9403..10218)
                     /locus_tag="POH73_000564"
     CDS             complement(9403..10218)
                     /locus_tag="POH73_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HDI6886541.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TVVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILHHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDRRILEVLVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMKKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
     gene            10513..10938
                     /locus_tag="POH73_000565"
     CDS             10513..10938
                     /locus_tag="POH73_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDI6886542.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTMDGY
                     HTVPYTISVDGITALHRTYFVFPENKKVLYQEIDSKVKNQLASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            complement(11329..12033)
                     /gene="budA"
                     /locus_tag="POH73_000566"
     CDS             complement(11329..12033)
                     /gene="budA"
                     /locus_tag="POH73_000566"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDI6886543.1"
                     /translation="MASVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGAVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDEDFIN
                     ANIDYKDIADEIREAE"
     gene            complement(12070..13734)
                     /gene="alsS"
                     /locus_tag="POH73_000567"
     CDS             complement(12070..13734)
                     /gene="alsS"
                     /locus_tag="POH73_000567"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDI6886544.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSADE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            complement(14204..14386)
                     /locus_tag="POH73_000568"
     CDS             complement(14204..14386)
                     /locus_tag="POH73_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886545.1"
                     /translation="MIDSVFWKKAFIPVYFIVAMLVFLLFRFYIKTNNFSIYLMIIFL
                     MCLETASIIYNYKNNR"
     gene            complement(15036..15215)
                     /locus_tag="POH73_000569"
     CDS             complement(15036..15215)
                     /locus_tag="POH73_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886546.1"
                     /translation="MTEIVEFLSDGKLASRLEIQETMGLQKSYTSELIAELKKLEIIG
                     SEGRGPGTRYYLIRD"
     gene            complement(15464..16900)
                     /locus_tag="POH73_000570"
     CDS             complement(15464..16900)
                     /locus_tag="POH73_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDI6886547.1"
                     /translation="MTSSKYIEYLQTEVEGQLLDRKRASINPKDVAQHISAFANAEGG
                     KLVIGIEDNGEISGFNHSKAKKKEVYIEAPFEHLYRIPKYTYEIIEVKKRDGTMDEIL
                     IFDIEPSYDAIITLKDDSVYLRINDKSRKLTHNQITNLEYDRGSRRFEEELVEYSSIE
                     DVNEELVDEFKQLLNTNVDNEKLLKARGFMREGKLTVAGLLLFSNHINVYLPGARIRF
                     MRYEGTKEESGTRLNIVKDITFDKALPVAIREARAFINTQLREYTFLGKEGRFVTLPE
                     YPEFAWFEGMINSIVHRRYDNQGDHIRIKMFDDRLEISSPGALPASVTLENIKEERYS
                     RNPKLAAALTQYKWVRESNEGVGRIFDEMKQYFLDDPVYSEPNNSSVQLALKNNIIAR
                     KKRETGRVSNIITPELFESLNEQQELIVRHLYNQGELTASKIANIIQRSVPTARKRLK
                     ELEEMNIISTHGSSKNDPKRTYYLLGFE"
     gene            complement(17249..17647)
                     /gene="rpsI"
                     /locus_tag="POH73_000571"
     CDS             complement(17249..17647)
                     /gene="rpsI"
                     /locus_tag="POH73_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDI6886548.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            complement(17661..18098)
                     /gene="rplM"
                     /locus_tag="POH73_000572"
     CDS             complement(17661..18098)
                     /gene="rplM"
                     /locus_tag="POH73_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDI6886549.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            complement(18347..19141)
                     /gene="truA"
                     /locus_tag="POH73_000573"
     CDS             complement(18347..19141)
                     /gene="truA"
                     /locus_tag="POH73_000573"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDI6886550.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRIHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTDLNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQQRDPFQSGLKTFIPESLDLDKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSL"
     gene            complement(19146..19952)
                     /locus_tag="POH73_000574"
     CDS             complement(19146..19952)
                     /locus_tag="POH73_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDI6886551.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWMFALILFFMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTLSLIMIIPIAIILFVLKYSGV"
     gene            complement(19942..20802)
                     /locus_tag="POH73_000575"
     CDS             complement(19942..20802)
                     /locus_tag="POH73_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDI6886552.1"
                     /translation="MIIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKTIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKEKLADWHIGLPEIVQLQYDFEQKHQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            complement(20799..21608)
                     /locus_tag="POH73_000576"
     CDS             complement(20799..21608)
                     /locus_tag="POH73_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDI6886553.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPHDEMHRRVSEALEQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDHAEELTTIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            21984..23551
                     /locus_tag="POH73_000577"
     CDS             join(21984..22659,22659..23551)
                     /locus_tag="POH73_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611580.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSau2 family transposase"
                     /protein_id="HDI6886554.1"
                     /translation="MKTLKGALFMSIKPKVDIDVLLYMFQEYEKGVSFQYLIDTLQLD
                     INVNTLYPKYKYYKTHGIETLLTQKQYNHYSKELKLKVVNEYLNSNQSTQDIAIKYNI
                     RSSTQVKNWIKMYTVGKEIKNQSPKTGVYIMKARKTTFEERVTIVEYYLNHKQSYREV
                     AEHFNISYGQIYQWVHKYQAHGKNGLVDGRGKGKPKSMMTPEEQKEAEIQALKAQNRL
                     LEMENDVFKKVSSIGKRDDSKRKQITAYKTIEALKHQYPIQRLCRILGISRASYYKWV
                     HYQSSELELENEQLKREIESIYHKYNGIYGYRRIYIYIRLKLGKQVNRKRIYRLMKEL
                     NLKAVIRQKRKPYRRSTPQITSENKLNRQFDIDTPNKVWLTDVTEFKIKEGSKIYLSA
                     IYDLGAKRIVSYELGPSNNNQLVFKTFNQAIEKVENTKGILFHSDRGFQYTSKTFKHM
                     LDECGMIQSMSRVSKCIDNGPMEGVWGTIKSEIFRGNKHFKFNSVEEATKTIHDFILF
                     FNHERITLKMADSV"
     gene            complement(23804..24172)
                     /gene="rplQ"
                     /locus_tag="POH73_000578"
     CDS             complement(23804..24172)
                     /gene="rplQ"
                     /locus_tag="POH73_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDI6886555.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            complement(24189..25133)
                     /locus_tag="POH73_000579"
     CDS             complement(24189..25133)
                     /locus_tag="POH73_000579"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDI6886556.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            complement(25208..25597)
                     /gene="rpsK"
                     /locus_tag="POH73_000580"
     CDS             complement(25208..25597)
                     /gene="rpsK"
                     /locus_tag="POH73_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDI6886557.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            complement(25621..25986)
                     /gene="rpsM"
                     /locus_tag="POH73_000581"
     CDS             complement(25621..25986)
                     /gene="rpsM"
                     /locus_tag="POH73_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDI6886558.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            complement(26009..26122)
                     /gene="rpmJ"
                     /locus_tag="POH73_000582"
     CDS             complement(26009..26122)
                     /gene="rpmJ"
                     /locus_tag="POH73_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDI6886559.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            complement(26154..26372)
                     /gene="infA"
                     /locus_tag="POH73_000583"
     CDS             complement(26154..26372)
                     /gene="infA"
                     /locus_tag="POH73_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDI6886560.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            complement(26565..>26651)
                     /locus_tag="POH73_000584"
     CDS             complement(26565..>26651)
                     /locus_tag="POH73_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDI6886561.1"
                     /translation="QKGVLKNIDGSKDISDVTKDVIDILDHL"
BASE COUNT         8585 a         4706 c         3904 g         9456 t
ORIGIN      
        1 atgacttcat gtccttttga ttttaaaaat tcagatacac gcatttttgt atctgtaaca
       61 atatggtcgc atcctaatgc aatcttcata gtaatttttc ctccttagca cattttatta
      121 agcatatcta cgcggatttg gtgtctacca ccatcgtatt taccttcaac aaaaccttta
      181 acgacatttt tcgctaatgt gtctccaaca atttcagatc ccatagtgat cattcttgaa
      241 ttgttatggc ctctagtcat atatccagag cgttcatctg atacttcagc agcaatcatg
      301 cctttgattt ttgtagcaac cataaagcta cctgcaccaa atgcatcgat aacaatacct
      361 aagttacctt cttgactttg aacatctttt gctacagcca aagttgcatc aacaaagtca
      421 acttcctgtc cttctgttac gtcaacaacg tcatatttat tgtctaataa gtatgatttg
      481 atgacttctt ttaatcgttt gccagcttca tctgaaccaa taataatcgc cataataaga
      541 ctccttttta ctttaatttt gaaatacctt tcttaaaata tgacatattt atttgtaggt
      601 tatgaaaatc ttgagaaaag gctttcaatt tgattacgtt taaattataa acataaacaa
      661 acaataaatc aacataatat gtttataatc tgtttgttta tgacatattt tcaaacaata
      721 agtgaacatt cttattgtgg tgttgtttta attaggtatt cgtctgaaat tgtagtaaaa
      781 atttgtcggg gttcccgttt acataaattt gcaaaaaaat agcccataaa tgaatgcaaa
      841 ttcacattca cttatgagca tatagacaca tattttaaca atgcagttat atttttaatt
      901 tagtcgacta cttcaatata tgttttaatc gtttctactt tttcttcatc ttcatagtcc
      961 atgaccactg cagtcaattc gtttaactga caaaatgatg taaaatcttc tttcccaact
     1021 ttcgtatgat caattagcaa gtatttttca attgaattac ttagtgccaa ttgttgcgta
     1081 taagcttcat ctaatgtaga tgtcatcaca gcacctttat ttactgcgtt actactaaag
     1141 aacatcttgc taaatcttag tttttctaac atggcattcg ccatttcacc tacaaatgct
     1201 tctgtaatat ggcgcatttc accaccaatt aaatagacac gaaaatgtgc tgtttgtttt
     1261 tccaacaaaa ttttatacac cggcaaacaa ttcgtaataa ttgtgagcgt atgatgattg
     1321 acttcttctg ctaatagttc cactgttgtt cctggtccga aaaacaaagt atccccatct
     1381 tcaattaatg atgcagcttt tctagctata aatcgttttt ctgcaatttg acgggtatgt
     1441 ttttctttat gcgatatttc tttatactga aatgttgaat tactgcgtgc accaccatga
     1501 atcttcgtta aaatcccttt attttccaat tcaattaaat ctcttcgaac tgtcatatca
     1561 gacacattta aaccttcgac gatttcattc gttcttatcg tgcccttttt attcactagt
     1621 ttagcaattt cgtccaaacg ttcatgttta ttcaatgtaa aattgcctcc tttatttagg
     1681 cgatgggtca ttatccttta tgtttagaca tcaattttaa tatacatgct tattctataa
     1741 taaaaattgc gtaaaaacta atataattta actgttcgat tatatgtata attcattttt
     1801 aaaaagaact gtttaaactg cgcttttgat aatcgtaaaa tgaatatact ataataaaag
     1861 caaaagacgt tataaatcat tcattatttt gatggggtgt ttaacttgaa acatgattta
     1921 gagacactaa aacatattat agatagttcg aatcgtatta cattttttac aggtgctggt
     1981 gtatctgtcg caagtggtgt tccagatttc cgttcaatgg gtggcttatt tgatgaaatt
     2041 tcaaaagatg ggctttcgcc agaatacttg ttaagccgtg attatttaga agatgatcct
     2101 gaaggtttta tcaatttctg ccataagcgc ttactatttg tcgatacgat gcccaatatc
     2161 gttcatgatt ggattgcaaa attagaacgt aatcaacaat ctttaggtgt catcacgcaa
     2221 aatatcgatg gtttgcactc agacgctgga agtcaacatg ttgatgaatt acatagcacg
     2281 ttaaatcgtt tttattgtaa tgcatgtcat aaatcttata tgaagtcaga tgttattgat
     2341 agaactttga aacattgtga taattgtggt ggtgccattc gaccggacat cgtattatac
     2401 ggtgaaatgt tagatcaacc taccatcata cgggcattaa ataaaattga agatgctgac
     2461 acccttgtcg tactaggttc atcactcgtt gtacaacctg ccgcaggatt aatatcaaat
     2521 tttaaaggcg acaatttaat tattatcaat aaagatcgaa caccttatga taacgacgct
     2581 actttggtca tacatgacga catggtttcc gttgtaaaat cattaatgac agagtaatca
     2641 gtacgtaaaa agcatttccc gagtaacagg gaaatgcttt taattttgaa gttgcgtatt
     2701 cattttttga gcttggaatt cggagtttgg ttttcggaat tcgacgtttg gaattccaaa
     2761 ttaaaatgag tgagaaattt tatttattgc gtgcttaacg atttggtttt gtagcgattt
     2821 ttatttgatg atttgtgttt tatttgatgt tgggatgcta cttttaatgg tttgtcattt
     2881 gttagattaa aaaatcagat atcttcgtta tcaaattcca atattaaatc attatatttt
     2941 tcagaatatc ttgcataatc ttcattatta ttaaaagcta accatagacc tctatcatca
     3001 tataaatgat atattaagtt tttagtattg ttaattatat atatttcaga ataattatca
     3061 ttctgattga tatttggaac taacccttta aagtcttgat tggatatagc ttttaataat
     3121 tttttatatt ttatatcttt tatggaacac ttatatgaaa actcctcgca caaaatatta
     3181 tcatccttct cctttttata ttctttaaag taatacaatt catctattgt taacgatgta
     3241 aattttttta taacatttgt tctcttataa ggttcattat aaacatatac tacatgaacc
     3301 aaatttatag aatcagaagg cgaaaacaaa tcttcaaaaa tgtttattgc attatcatat
     3361 acagctaaaa aataagtatt attgatagtt ttaccgtcct ccaaaaactg gtatttatcg
     3421 ccacttaaac ttaaatgaat tgcgtttttt acctttctaa aattcaactt ttttatatca
     3481 aaattattta tatcaaagca cataatttac aacaccttat tcccttaaga tttttccttc
     3541 tttagttaaa gaagctattc tttttccttt gttatttatt aacttccaat aagaaccacc
     3601 atatgattgc cctttatttt tggaaatagt ccatttaatt tttggatatc tccaggtagg
     3661 atttttccct ttaagctttt gaataaattt accaatactc acccccttat catttaacaa
     3721 acttttatta acgtttttgg ctatattatg agtctttact gcttttagat tgtaaaaata
     3781 atcgtcatga ataatggcat tttccgacat tcaaatttta caaagtacat tgtcatatgc
     3841 ataatgatac ctgtaattaa tgtaataata gaaaagggta tatatgtcat atcgccacct
     3901 gctatacaac ttattagata attaattcca agacatatta attatctaat tataacctta
     3961 ggtaagttat tgcataatat aaaaattcat aatagcttat ttgcttgctc ctttcacttt
     4021 ttaagagtaa taaattaacc tgaaacatag actttaacat ccaagtttca ggttagcgta
     4081 atgtaaatta tttactttgt tatattatga ttaaacatct aattctctgc ttgtaagtgc
     4141 tttaatcatc tctggatcag catgtgagaa aaattggcta ttactttctt ctaatgtcgc
     4201 aatttgttgt ttatcttctt ctgtaagttc aaaatcaaat atatctaagt tttgagccat
     4261 acgttctgga ttcactgatt ttgcgagtac aacgatgtca cgttctacta accaccgtaa
     4321 aataacttgt gcaattgatt tgttatattt atctgcaatg gcctgcaaca attgattttg
     4381 gaataattga tttttacctt ctgcaaatgg tgcccaagct tctaccacaa cattttcttg
     4441 ttgaagtgct gcaacttgtt cttcttgttg atggaatgga ttaatttcta tttggttcac
     4501 ttgtggttga atttgattgt gaatacctaa atctacaatt cgatcaacgc cgaagttaga
     4561 tacaccgatt gctttaattt taccagtttc ttttaactct tctaatgcac gccatgagcc
     4621 atatacatca ttgtaaggct ggtgaataag cactagatca atatagtcca aatttaatcg
     4681 ttgtaatgat cgttcgattg atttgatagt atcttcatag ttaacatttt caatccatac
     4741 tttagtcgta atgaatagtt cacttctatc aatacctgaa gcttcaatac cttgacctac
     4801 ttcagtttca tttaaataac tttgtgctgt atcaatatgt ctataacccg ctttaattgc
     4861 ttctttaacc gcttctgctg tttgttcttg aggaatttga aatactccga aacctaatac
     4921 tggaatcttt acatctttac ttatttcaat atgattcata aaatgaacct cctttatctt
     4981 gtaagtccac tataaaccat ggagcaaact ctatagcaag ccagaggttt actttctctc
     5041 ccaaagacgt tcaacattgt catcatgatt atcactagca ttgattttag caatatgatt
     5101 atcataatta tcaattttat attgtagtaa atctctcgca tcatgaattg tctttaagtt
     5161 ttcatttaat tcttcgagtt gcttatttaa aatttgcttt tgctgtgctt gaatattttc
     5221 atttttagga agctgcgcta acctacaaaa ttcgataagc gattcaatac tgacgcctgc
     5281 atttcgcaaa tttttcacta aataaatcca atttaaatca ttatcattgt atattctata
     5341 tccattttca tctcgattaa caggtggaat cacaccaacc ttttcataat atcttaaagt
     5401 gtcttgagat atattcatga gcgctacgac ttctttagtt ttcattgtga ctatcctttc
     5461 agttatgttt ggttgtctaa agtaatgtta ctttatatat tgtcatcttc gtttgaatac
     5521 ttcttatttt attactcaaa tttaaatttg tctctttttt aacattttac atttcatcgt
     5581 ttttaattac tttaaaaatt gtataactta aatatttaaa atgatataat cacaaagatt
     5641 gataatattt aatttttaaa aaatattttc gttgcaattt ttgggtgatg ctgaaaattt
     5701 gggtgatcga ttgtttatat gcatcaacat tctttgtgga ttgtttgaca gtagacagtc
     5761 aattgtatac gttaacgaat tttgaaaaat tgaatcatta tttaatcata atattcattg
     5821 cgcacgcgca acacaaaaag gagacatgtc aatgacatat agaatgaaga aatggcaaaa
     5881 attgtccacc attacgttat taatggctgg tgtgattact ttgaatggtg gtgaattcag
     5941 aagtattgat aaacatcaaa tcgctgtggc tgatacgaat gttcaaacga cagattatga
     6001 aaagttgagg aacatatggc tggacgttaa ctatggttat gataagtatg atgagaataa
     6061 tccagatatg aagaagaagt ttgaggctac ggagaatgag gcagagaaat tactcaagga
     6121 aatgaaaact gaaagtgata ggaaatactt gtgggaaagc tcaaaagatt tagatacgaa
     6181 gtctgcggat atgactcgta cctatcgtaa tattgagaaa atctcagaag cgatgaaaca
     6241 taaaaatact aaattaaaaa cagatgaaaa caagacaaaa gtaaaagatg cacttgagtg
     6301 gctgcataaa aatgcatatg gaaaagaacc agataaaaaa gttgctgatt tgacctcaaa
     6361 ctttaaaaat aaaacttcta gaaataccaa cttaaattgg tgggattatg aaattggaac
     6421 acctagagca ttaacaaata cgcttatact cttacaagaa gatttcactg atgaagaaac
     6481 gaaaaaatat acagctccta ttaaaacttt cgccccagat agtgacaaaa tattatcttc
     6541 tgtaggaaaa tctgaacctg ctaaaggcgg aaatttagta gacatttcta aagtaaaact
     6601 tttagaaagt attatcgaag aagacaaaga tatgatgaaa aagtctatag attcatttaa
     6661 tacagtcttc acttacgcgc aaaattctgc cactggaaaa gaacgtaatg gattctataa
     6721 agatggctct tacattgatc atcaagacgt cccatacact ggtgcttatg gcgttgtact
     6781 attagagggt atttctcaaa tgatgccgat gataaaagaa acacctttta atgatagtaa
     6841 ccaaaatgat acaaccttaa aatcatggat tgacgacgga tttatgccac tcatttataa
     6901 aggtgaaatg atggatttat caagaggtag agctatcagt cgtgaaaatg aaacgagtca
     6961 ctcagcatct gcaacagtaa tgaaatcatt gttgagattg agtgatacca tggataagtc
     7021 tacaaaagct aaatataaaa agattgtcaa gacttcagta gagtcagatt caagttataa
     7081 acaaaccgat tatttaagct cttattcgga tataagcaaa atgaagtctt taatggaaga
     7141 cagcactatt tctactaacg gtttaacaca acaacttaaa atatataatg acatggatcg
     7201 tgtcacctat cacaataaag gcttagactt tgcatttggt ttaagtatga cgtcgaaaaa
     7261 cgtcgcacgt tacgaaagta tcaacggaga gaacttaaaa ggttggcaca ctggtgctgg
     7321 aatgtcttat ttatacaata gcgatgtgaa acactaccgt gataacttct gggcgacagc
     7381 tgatatgaaa cgtttagcag gtactacaac tttagataat gaagaaccta aaagtacgga
     7441 tgttaaaaag tctagtaaaa cttttgtagg aggaacaaaa ttcgatgacc aacatgctag
     7501 tatcggaatg gattttgaaa atcaggacaa aactttaact gccaaaaaat catatttcat
     7561 attaaacgat aaaattgtct tcttaggaac tggcattaaa agtactgatt catcaaagaa
     7621 tccagttaca acgattgaaa atcgcaaagc gaatgattat aaattatata aagatgatac
     7681 gcaaacaacc aattccgata atcaggaaac caattccctc tttttagagt caacgaatag
     7741 cactcaaaac aatataggtt atcatttttt aaacgaatcg aaaataactg taaaaaaaga
     7801 aagtcatact ggtaagtgga gtgatataaa taaaagccaa aaggatatac aaaaaactga
     7861 tgagtattat gaagtaactc aaaagcattc taatacagat agtaaatatg catatgtgtt
     7921 gtatccaggc ttatctaaag atgtctttaa atccaaagca agcaaagtaa ctgtcgttaa
     7981 gcaagaagat gacttccacg ttgtgaaaga taatgaatcg gtttgggctg gtatcaatta
     8041 tagtgatagc gctaaaactt ttgaaattaa taacactaaa gtcgaagtta aagccaaagg
     8101 aatgtttatt cttacaaaga aagatgataa cacttatgaa tgtagcttct ataatcccga
     8161 atctacaaat tccgtttcag atattgaatc taaaatttca atgactggat actctattat
     8221 aaacaaaaat acgtcgactt ctaatgaatc cggcgtacgc tttgaattaa ctaaataagc
     8281 aagaaaatct cggcactata ccatttttat agtgtcgaga ttttataagc ataactatca
     8341 ttcaccttgt aatccgcaga ccacatctcc tttgataagt tctcgattat ttataaatcg
     8401 aatttttaaa gatgtacatg cttcaatatt cttatcattc attacttgaa aatgtatatg
     8461 aggttcagta gaattgccag aattcccaac cttcccaatg atgtcaccat atttaacatt
     8521 ttgcccctca ttcacaatga ttgaatattg ttgtaaatga gctattaagc tatactcatt
     8581 ttctgcgtgc ttgattacaa tataatttcc taaaaactgt gattccttcg tacttccaat
     8641 actgttgtct tgaaccccat tcactacttt ttcaactgta ccatttgctg gcgcaatgat
     8701 atcttttcca aaacaaaaat aatcagcatt tacttttcct aggttcttat atgttttatg
     8761 attttcttta ataactaaat caatagcata cctttgatgt gggtataaat agtgataatt
     8821 atataattca ttatctccac cccaaaaaac ctcatagcaa tctgttgtcg gcactgcgta
     8881 ttttaaagtc gtttcattct taatcgcatc ataattgatt aacggacgca taacaaatcc
     8941 gataatacta ttatctttcc atgtcactgc cactccaaat tgatttgctt tatctatcca
     9001 tatttgctca atagtaccat cattttgagt acacactttt aattcatgta catcagactg
     9061 ccatgtataa gatttgaaaa gtttcctaaa ctgccaataa ctaaactctc gcttcaaatt
     9121 ttgatcaaaa atcttatata ttttcctgct cttactttta tttaaataat ccccaaagtt
     9181 tgttttattc atatatactt aacctcttat attaattttg gtatcaattc tttctccagt
     9241 tcctgcgtca tttttgcgct acctgaatcg tctacactta attggacaaa tcaacatata
     9301 aataattttt taaaactaaa gtgtagtcga ttcactatat tttctaatca tcccttaaat
     9361 attaatattt gtacttaaaa agtatacata tcactcaacc aatcaataat catacttacc
     9421 ttcaacaaat tctttaacat gcagtgttat cccttgataa tatggatatt ttgttaatct
     9481 aaatttctgc tttaatgctt catctctact attcgacatc aaataggcat gttcaagata
     9541 gcttaaatat gcttcgtcat tgccgctatc tccaaaacct aatattgatt ttactggtat
     9601 attatatttc ttcatcagaa attgagtagc atatagtttt ccagcatttg acggtgtgaa
     9661 gtcaatatca tacgcatttt caggatcacc agctaatgga ttgcttcgac taaatctcgc
     9721 tgtatagttt tggtcatttg cgtatctgac aagtacttct agaatacgcc tatcattgtt
     9781 gtgattacct gttgaatgaa aataaaaatt ataatgtgtt tcattacctt caaatgctct
     9841 ttgtggaata aattcgacat tatattgcgc agaaatatga tgtaatatat cctcaatatc
     9901 ttcaaataaa aatggttcgc tcaagacttt ctttttatat gtttctgatt caacatattc
     9961 gccattattc caagtgaaca ttttagaact caaatcagaa aagatatgta atggtttaaa
    10021 cgtcatatta acttgatcca ttttatccat gacactttga aatgtacttc ctgtcaatac
    10081 aacagtgatc acttcattat tattagttaa tttttccaac aatttttcca tttctcttaa
    10141 atgacttaaa tcttcttcat ttgtattatg tttaaaatat gtttcatcaa aatcaaacaa
    10201 taatagacgc tttgacatac tcattcctcc ataatatttt aatgaattta tctataactt
    10261 aaatacacta taagtctcaa ttaaaagtat aagatcgact atatgctttt catgcttatt
    10321 atatacaact aatgaatccc tataaactaa aaacattgct taccactttt caaaatacac
    10381 gggtcatatc cctcgttaaa aactattact aaaaatcaaa aatataaaaa aatcaaaaag
    10441 tatgttttaa actttatgaa acgtgttata taaatagtgt aatataccac aactttaaaa
    10501 ggagattgag atatgaaact aaaatcattt gttactgcca ctttagcatt gggcttatta
    10561 tcaacggtcg gagctgcatt acctagtcac gaagcatctg cagatagtaa taacggctat
    10621 aaagaaatga caatggatgg ttatcacact gttccttaca cgatttcagt agatggtatt
    10681 actgcattac atcgaactta ctttgttttt ccagaaaata aaaaagtact ttatcaagaa
    10741 attgacagta aagtaaaaaa tcaattagct tctcaacgtg gtgttacaac agaaaaaatt
    10801 aataatgcac aaacagcaac gtatacactt actttgaatg atggtaataa aaaagtagtg
    10861 aatctaaaga aaaatgacga cgctaaaaat tcaattgatc caagtacaat caaacaaata
    10921 caaattgtag ttaaataata atttgaaaat gaaaatagtt atacagggca aacgctgaaa
    10981 aattgcgttt gtctttttag tatgaaatga ataatcgaac ttgtaattta ttattgattg
    11041 cttctactga caatagatta gattggccta tactaaagta aattctatat taacgtcttc
    11101 cgtaatttat aatctaacat aacagcaaat tacaaatagc tagaacataa ataaatcccc
    11161 tcactaccgt taatagtgaa gggatttatt ttattacata gacgtatgct ctatgacaac
    11221 catgcttaac cgtattacac aaccttttat cattgctaaa tttccaaatc gaacattatg
    11281 aagtcaatgc ttcaattttt ataatccatc attacatttc cgcattcatt attcagcttc
    11341 tctaatttcg tctgcaatat ctttatagtc aatatttgca tttatgaaat cttcatcttg
    11401 aattggaaaa tgctgttcaa atgtttctat attttggatt tctaccttaa catcttctac
    11461 ttcaaaatct aagacatgtc caccaaagtt acgatcatca tttgcaaagt gtacatggaa
    11521 tcctgctgac ccgataccat ggaatagttc tggtgtaaag aacccaacga ctgcgccttt
    11581 gacgtacgtt tcagtttgtt caggttgtct tctagctgag tcaattaaac gtgtataagg
    11641 tggctcttgt gccggcatca tacgtacatg cattttttta aataagcctg aaatttttac
    11701 tgcagaaaat aaattttcac tcaacatctt ttccttaatt tctgcaaaaa ctgcttcaga
    11761 agatttatct ttcgtttcat agcttgtatc tgctacgaat ttagttacag ttgcatatgg
    11821 tgttaactca tcacctttta attccacaaa ttctttatgt tcatttgcat gataggcttt
    11881 tccatctaaa aagatgacct caccatttga acctgttagt gtagcgatac ctaaatcacc
    11941 atgttgcaat aattcattta ttgatgcagt tccttttaat aaacctgcca ttaatgtgcc
    12001 taatgtacca tgttgatata aaacactagc cataaaattt cccactcctc attaacattt
    12061 atctttgttt tagttaaata cgtcaggtaa catatttgtt gaaagtttga tattatcttt
    12121 gtaatttaca ggtatatcaa ttaatactgg accatctgtt tcatagccct ctttaattgc
    12181 cgcttctaat tcttcttgat tagtaactcg taaacctttt gcgccaaatg attcagcata
    12241 ttttacgaaa tctacaggac cgaagtcgac acctgatgaa cgtttatatt tcatttcttc
    12301 ttggaattca accatgttat attttccatc gttccaaata agttgaataa tgtttagatt
    12361 tttacgtacg gctgtttcta aatcttgtga tgaaaataaa aagcctccat cgccagcaac
    12421 ggatacaact tgcgtattag ggcgtacaag cgcagctgaa attgcccacg gtaatgctac
    12481 accaagtgtt tgcataccgt tactaaataa taaatgtctt ggattgtaac ttctgaaatt
    12541 acgtgccatc caaatatagt gacttccaac atcaactgtc actgttgtat catcagttaa
    12601 taccttttgc atagattcaa taatttcaac tggatgtaga attccttctt catgcgtcgc
    12661 tttaatacca gtttcttcat caatatgtgt tcttaattgt tctaattcgt ctaaatgttg
    12721 ttgatttata aatggctcat ctactttttc agaaatcatt tcaatcgttt tagcaatatt
    12781 accaatcaac tcttttttcg gttgcatata attagtaatt tcagcttgaa cttcgtcaat
    12841 attgataatt tgtgtttcta attctttatt ccagttacta gcttcgtatt caattggatc
    12901 ataaccgatt gtaacaacta aatcactttt acgtaataat tcatcaccaa cttgattacg
    12961 gaataagccc acacgaccga agaaatgatt ttctaattca cgactaatta cacctgcacc
    13021 ttggaatgtt tctacaactg gtaaattcgt acgctctact agcttgcgaa tggcattcgt
    13081 ctcgtcagca ctagaacttc tcataccagc taataaaaca ggaaatgatg catttttaat
    13141 cgcttcaatg acatcattaa tatcttgttc actcggtact cctaaatttg gtttttggca
    13201 aagtgatata gctttagatt caactggtga agaaatcacg tcttgcggaa tactaataaa
    13261 acttgcgcca tttttccctg aagtagcaat acgaattgca tttgtcataa cttctgataa
    13321 tgattcagga tcttgtactt cttcactgta ttttgatgag tattttaata gcgcagcatt
    13381 atcaatactt tgatgcgtta atcgtaataa atcattacgt ttcacttggc cgcctaacgc
    13441 caagacagga tccccttcag atgtagctgt taatagtcca gtcgttaaat tacttacacc
    13501 aggaccactt gtaacaagta ctacacccgg tttgcctgtt aatcttccaa taccttgcgc
    13561 catcattgcg gcgttttgtt catgacgagt gacaataagt tcaggaccat catcaattaa
    13621 agcattaaat agatagtcta tctttgcacc cggaatacca aaaacatatt ctaccccatt
    13681 atttttcaaa gtatcaataa ccatatcggc tgcagtgtac tttttatcag tcatttatat
    13741 tcatttccct tcaaatgtga tgtgtagatg tgtattacat gtaattacat tgtgatttgt
    13801 aatctaatga tagttatgta aaattaatga tatttatcaa aactttacat atacaattag
    13861 atttgtgata cataaaaatt gccttcagta cattatatat cctttgtaag atgaataaaa
    13921 atcgttattt gtaatactaa gtattccaaa aatgcatgag tagattttga cgggagtgat
    13981 tataagggat gttggattga ttggtgtgga ggattgttag catatttgag aatgaggtct
    14041 ttagtcaatg acggtttgaa attgtcgttt tatggcgggc tgaaaatgct gttttatgac
    14101 ggtttgaaaa tgctgttttt ggcgatttaa aattgtcatt tcgataaaaa aagccacagc
    14161 aagacttaga tccaactgtg acttatacat ttcatactta tatttatcga ttgtttttat
    14221 agttataaat gatagatgca gtttctaaac acattaagaa gataatcatt aagtatatag
    14281 aaaagttatt tgttttaata taaaacctaa aaagtagaaa caccagcatt gcaacaataa
    14341 aataaacagg aataaaagct ttcttccaaa atacagaatc tatcaattaa gtcactcctt
    14401 attttgataa cactcttaaa tcatggttct tgagctatct tccgattttc atccgatagc
    14461 cctttccctt gaaccactat gattaaaaca ttattgtccg tcctccgcaa gctaaatgac
    14521 aatttaataa tgtacacttt tgattgtgta agcatgttca aattatacgt gtaaatcaat
    14581 aattcatagt tgaatttatt ctattcctta gacattaaat aaaggaaaat tcattatact
    14641 ctacacattc catattcccc acacaaacgt aaaaaaacac ctcgacataa tatcgaggtg
    14701 ttttgtatat atcgtccgac aattaacgtt ttgagaattg aggtgaacga cgagctgctt
    14761 taagacttgg ttttttacgt tcaaaaaact ttagggtttc gtacaattgt atagactgtc
    14821 gtaagccttg atactaaagg attcctaatt ttttcgttca catagaattg catcaaatat
    14881 cacctagggg taactaaagg gtaactaaat aaactgctgt ccggtggtat atactctcgt
    14941 atcatccggt tatcgtccgg tcgtaaatat attgatttat caatgttttt atgtatatca
    15001 ataggttatc gtccggttat cacttgatga atttattagt ctctaattaa ataatatcta
    15061 gttcccggac ctctaccttc agatccgata atctctaatt tctttagttc agcaattaac
    15121 tcagatgtat aacttttttg aagtcccatt gtttcttgaa tttctagcct acttgctaac
    15181 ttaccatctg acagaaattc tacaatctct gtcaacttct gattagtagg tattttattt
    15241 aacttgtaat tcttattaaa tagtactact cgaaaatccg cctctttact cgaaagctca
    15301 caaaactctt gatatcactg gtttctcatg attttttaat gtttttattt tgagttactc
    15361 gaaaattttc ctctttactc gaaagctcac aaaacccttg atatcattgg tttctcatga
    15421 ttttttaata tttttctttt gagttactcg aaaatttttc tctttactcg aatccgagta
    15481 aataatatgt tctctttgga tcattcttac ttgatccatg tgtcgatata atattcattt
    15541 cctctaattc tttcaatctt tttctagcag taggaacaga cctttgaata atattagcaa
    15601 ttttagatgc tgttagttcg ccttgattat ataaatgtct cacaattaat tcttgttgct
    15661 catttaaact ttcaaataat tcaggtgtta ttatattact cactctccct gtttctcttt
    15721 ttttccttgc tattatgtta ttcttcaaag ctagttgtac tgaactatta ttaggctctg
    15781 agtatacagg atcatctaaa aaatattgct tcatttcgtc aaatatacgt cccacgccct
    15841 catttgattc tctcacccat ttatattgag ttaatgctgc agctaatttt ggattccttg
    15901 aatatctttc ttctttaata ttctctaacg taacagaagc aggcagtgca cccgggctac
    15961 taatttctaa tctgtcgtca aacattttta ttcttatatg atcgccttga ttatcatatc
    16021 tgcgatgtac aatagaattt atcatcccct caaaccaagc aaattctgga tattctggca
    16081 aagttacaaa tcttccctct ttacctaaaa aagtatattc acgtaattgt gtattgataa
    16141 aggctctcgc ttctcttatt gctactggca aagctttatc aaaagtaata tcttttacaa
    16201 tgttaagtct tgtcccagat tcttcttttg ttccttcata cctcataaat cgaattctag
    16261 cgcctggtaa ataaacattg atgtgattac taaacaataa caaacctgca actgtcaatt
    16321 taccttcacg cataaatcct ctagctttta ataatttttc attatctacg tttgtattaa
    16381 gtaattgctt aaattcatca actagttctt cattcacatc ttcaatacta gaatactcta
    16441 ctaattcttc ttcgaatctt cgtgaaccac ggtcgtattc tagatttgta atttgattgt
    16501 gtgtcagttt tctactttta tcgttaatcc ttaaatatac tgaatcatct tttaaggtga
    16561 ttatggcatc ataactgggt tcaatgtcga aaataagaat ttcatccata gttccatcgc
    16621 gtttcttcac ttctattatt tcatatgtat atttaggtat tctatataaa tgttcgaaag
    16681 gtgcttcaat ataaacctct ttcttttttg cttttgaatg attaaatccc gaaatttctc
    16741 cattatcttc aataccaatt accagtttgc caccttcagc atttgcaaac gcactaatgt
    16801 gttgagcaac atctttagga tttatagatg ccctctttcg atctagaagc tgtccttcaa
    16861 cctcagtttg caaatattca atatacttac tactagtcat atataaacct cgcaatctcg
    16921 catcttattg actttattat aacattcaat actatatcta tcgaatatca ttacaacctc
    16981 tatctgcttg tcgtacgaat cccaaagaaa aaccaggtca tttcatatgg tttcctaaag
    17041 tttcatatca ttgaatttta agcagaattg aaacaaatta gttcctctag gggtaactaa
    17101 atagtatcta aatgactcta taaaaacaca aaaaacactc tttggaataa tccaaagagt
    17161 gctgtaaacg tttcttcagt tgaacacaaa ttgctgacgc aatttgcgaa ctccatctgt
    17221 aagcgaacaa gttcgcaaca gctgcagctt aacgttttga gaattgaggt gaacgacgag
    17281 ctgctttaag acctggtttt ttacgttctt tcatacgtgg gtcacgagta agtaatccag
    17341 cgcgttttaa agaacctctg tattcaggat ctgcttctaa taatgcacga gcgattccgt
    17401 gacggatagc ttgagcttgt ccagtgaaac caccaccatg tacgttaact aaaacatcat
    17461 agttaccttt agtttcagtt acatcaaatg gttggtttaa gtctaaaatt aatgattcga
    17521 atggtaagta ttcgcgtacg tcacggttat taactgtgat gttaccttca cctggtacta
    17581 aacgtacacg tgctactgag tttttacgac ggcctgtgcc tctatattca acttgtgcca
    17641 aagtcatttc ctccttctaa ttaaccacgt aattcgtagt tttctggttg ttgtgcagcg
    17701 tgtggatgtt cagcgccacc atatacaaat aattttttac cttgtttttc gcctaaacga
    17761 gtgcttggta acataccttt aattgaattt tcaattaaac gttctgggtt agttcttctt
    17821 aattcaccag cagtgattga tttgatacca cctgggtgat ttgagtgacg gtagtaaact
    17881 ttgtcagttt ctttgttacc agtaaattcg atttttgatg cattaataac gattacataa
    17941 tcaccagtat caacgtgtgg tgtgtaagtt actttatttt taccgcgtaa gatagatgct
    18001 acttctgatg ataaacgacc taatgtttgg ccttcagcat cgataacata ccatttgcgc
    18061 tcaatgtttg attcatttgc cataaatgtt tgacgcataa taattgtcct cctaaaaaat
    18121 aaatgcttct attcaagcta taaacagttt cataacctaa tagatgattt catttgtttc
    18181 atttttaaaa cgtttcattt catttatttt gttaactgtt gttcgtttct gcttatatat
    18241 cttgtaacac aataagtttc cggggcttat cgtggggtgg atataaaaca ataccgttaa
    18301 taatcttata atttcacaag ctttttgtca atggcaatca gtcattttat aatgattttt
    18361 tacgatgtat tttgatatcg ttaccaaagt cttttaataa ctcattttca tctaaataaa
    18421 ttttctctaa atataatcct tcagccggtg cagtgaaagg tacattctta cgattctttg
    18481 attcaagaag ttttggtaca tctgatactt catgtcgccc ttttccgact tctattaaaa
    18541 atgcaactaa gacacgcacc atgttatata aaaatccaga tccagtgaca atataatcaa
    18601 agccatcatc tgtctttaca atttcacttt ggtataatgt tctaaccttg ctttctactt
    18661 cagttttttg cgaacaaaaa cccgtaaaat catgtgtacc tatgaattgt tgtgccgctc
    18721 tgttcatttt gtctaaatcc aatgattcag gaataaatgt tttcaaacca ctttgaaatg
    18781 gatcgcgttg ttgtgcttga tatactttat atcgatagcg tttaccgaca caatcgtaac
    18841 gacaatgaaa atcgtcatcg actgtaacta cattgttgac ataaatatca tcaggtaacg
    18901 ttcggttcat tgcatattgc cattgtgaca taggtatatt caaatctgtg tcgaaatgaa
    18961 agtactgctg tatcgcatgt acacctctat cagtcctact tgaaggatgg attctcacat
    19021 gtcttttatg aatgcgttgt aatagctttt caaattgttg ctgtaccgtt cgaccatttt
    19081 gttgaatttg aaaacctaga aaattatttc cttgatacgc aatttctact aatatacgca
    19141 tgaatttaca ctcctgaata ttttaatacg aataaaataa ttgcaattgg gataatcatg
    19201 attaaagata acgtatctct caattgccat ttaagctgtc tgtagctcgt tctcttaaca
    19261 ttggcatcat aacccctaac ttccattgcg accgctaatt cttcggcgcg ttggaaagct
    19321 gagatgaata gtggcactag taatggaata aatgatttaa tacgtgttgc aatgttccct
    19381 gaacttattt cagaaccacg cgacttttgc gccaaaatga ttttatctaa ctcatccatt
    19441 aacgtcggga tgaatcgtaa cgcaatggac attatcatac ttaattgatg aactggtaat
    19501 ttaaacatct ttagtggcgc aagtaatctt tcaaacgcat ctgttaaatc aattggactt
    19561 gtagatagtg tcattattgt tgcaatcatc acaatcccaa ttaaacgcag tgatatatat
    19621 agcccttcta aaataccatt agtttcaatc gtgatgccat gccattcaac taatacatat
    19681 ccacctttag ttaaaaatat atgcatcatt aatgtgaaga ttaaaaagaa aaatattggc
    19741 gttaaacctt tgattaggaa ccataattga atttttgcta atctcataaa gaataagata
    19801 agtgcaaaca tccaaagata tgttaatggt gaatgacaga aaaatattaa tataataaaa
    19861 agaaaaacaa acattaactt tgctcttgga tcaagatgat gaacaaaaga attaattggt
    19921 aaatatcgcc ctataatcaa tttatttttc atgttgccac tccttataca agcttacaaa
    19981 tgcttcttca gttaaggcaa tatcttttaa ttttgtttga tgtttttgtt caaagtcata
    20041 ttgtaactga acaatttcag gcaaaccaat atgccaatcc gccaattttt ctttatcttt
    20101 gaagagctct tttggtgatg tttgcgacac gatactacct tctttcatta cgatgacttc
    20161 atctgcataa cgtgcgactt cattcatatc atgtgaaatt aggataattg tcttattttc
    20221 atctgtttgt agtgacttga gtaatcgcat tacttgtcgt ttactttgtg gatcaagtcc
    20281 tgctgtaggt tcatcaacca cgataatatc aggattcatt gccaatatcg atacaatcgc
    20341 tattttacgc atttgtccac ctgacatttg aaatggtgat tgtgacatta catctcttga
    20401 aaagcctaaa tccatcaaca gacgatgggc atagtttttg gcttcatcta aattcatttt
    20461 aaagttttta ggtccaaata tcatttcacg ctctactgtg tcctcaaata attgagattc
    20521 gggaaattga aataccattc caattctttt tcttacaggt ctaatatatt tatctttggt
    20581 cttatgtgta atagtaatat catcaactgt aactgtccca gtagttggct ttaacagtgc
    20641 attaatattt tgtatcaatg tcgatttacc actacccgtt tgtccaacga tggcataata
    20701 tttaccttgt tcaaattctg tattaacatc atgaatagct tgatgctggt atggtgtccc
    20761 tttttgatag gtataactta cattgtcaaa gcgtataatc atagttgatc caccagccct
    20821 tcataagtta agaatgatgt ttggtgtccc agcatttgat ttattttgat tgggaatggc
    20881 aaatctagac ctattgttgt taactcttct gcatggtcga aaatttcagt cgctgttcct
    20941 tctttataga cagtcccttt attcataacg ataacatgat ctgcttccat cgcctcagat
    21001 aaatcatgcg taatagaaat gattgtaata ttatgttctg atttaacttt tctcactaaa
    21061 tccaataaat tttgacgtgc atcaggatct aacatagaag tcgcctcatc taatataatg
    21121 acagaggggt taagtgctaa tacacttgct atagccacac gctgcttctg tccccccgat
    21181 aatgcattag gttcataatc tgcacgttct aacatatcta cttgttcaag tgcttcgctg
    21241 actcttctgt gcatttcgtc atgtggaacc gcatgatttt cgagtccaaa tgccacatcg
    21301 tattttacaa ttgaaccaac aaattgatta tccggattct gaaatacaat tcctatgtct
    21361 tttcttaact tttcaaaatt atcatctgtt atagcttgat tattataaaa aatttctcca
    21421 gatttaactt tctctatgcc aatcattaac ttggcaattg tagatttccc agaaccgtta
    21481 tgaccaacaa tagatgtcca ctgaccttta ggtatattaa aagaaacatc tttcaatgtg
    21541 aaggatgcat cactttgata ttgaaatgaa acatttttaa atacaataac tgaattctta
    21601 tcctccactt gtctctctcc tttacgattc gtgtatctat catattttac aatatttata
    21661 aatcgctgta tatgacattg actgggtttg ctatatatta ctagtatttt ctgactcatt
    21721 tctagtcttt aaagtgttgt ttaacaacta atgataagga catttattcc tctctaacaa
    21781 ttatgtataa acgttaataa aataaatgat ttactagtgt tggccgcgtt tgattcaacg
    21841 ataatacttt cacttcattc agttctagtg aaattgatca aactagcttg atcatatttt
    21901 tagattcgca ctcaaaaaag tagatataaa gaaatcggac ttaaaaacat tgaaatgctc
    21961 ccttcaaagt agacattgaa aaaatgaaaa ctttgaaggg agctttattt atgtcaataa
    22021 aacctaaagt agacatagat gtactgctat atatgtttca agaatatgaa aaaggcgtgt
    22081 cgtttcaata cttaatcgat acattacaat tagatattaa tgtcaatact ttgtatccta
    22141 agtataaata ttataaaacc catggtattg aaacactact tacacaaaaa caatacaatc
    22201 actattcaaa ggaactaaaa ttaaaagttg taaatgaata tttgaattca aatcaatcaa
    22261 cccaagacat cgcaatcaaa tacaatattc gtagttcgac acaagtcaaa aattggataa
    22321 aaatgtatac tgtaggaaaa gagattaaga atcaatctcc taaaactggg gtgtatatca
    22381 tgaaagcaag aaaaaccaca tttgaagaac gtgtaacaat tgttgaatat tacttgaatc
    22441 ataaacaatc ttatagagaa gttgcagaac actttaatat ctcatacggt caaatttacc
    22501 agtgggttca taagtatcaa gcacatggta aaaatggatt agtagatggc agaggcaaag
    22561 gaaaacccaa atctatgatg acgccagaag aacaaaaaga agctgaaatt caagcattaa
    22621 aagcacaaaa tcgattatta gagatggaaa atgacgtttt aaaaaagttt caagcattgg
    22681 aaagagagat gattcaaaga gaaaacaaat cacggcatac aaaacgatcg aagcgttaaa
    22741 acatcaatac cctatccaac gcttgtgtcg tattttaggc atttctagag cgagttatta
    22801 taagtgggta cattatcaat cgagtgaact tgaattagag aatgaacagc ttaagcgaga
    22861 aatagaatca atctatcata agtataatgg tatttatggc tatcgtcgca tttatatcta
    22921 tattcgctta aaactaggta aacaagtaaa ccgaaagcga atttatcgtt tgatgaaaga
    22981 acttaattta aaagcagtaa tacgtcaaaa gcgaaaacct tatcgacgtt caacgccaca
    23041 aatcacatca gaaaataagc ttaatcgaca atttgacatt gatacgccaa ataaagtttg
    23101 gttaacagat gtaacagaat ttaaaatcaa agaaggtagt aagatatatt tgagtgccat
    23161 ttatgactta ggtgctaaac gtattgttag ttatgagcta ggcccttcta acaataatca
    23221 attagtattt aaaacattca atcaagcgat agaaaaagta gaaaatacaa aaggtatttt
    23281 atttcatagt gaccgaggtt ttcaatatac atcgaaaaca tttaaacata tgttggatga
    23341 atgtggcatg attcaaagta tgtcacgtgt tagcaaatgt atcgataacg gtccaatgga
    23401 aggtgtatgg ggtacaataa aaagtgaaat ttttagagga aataagcact ttaaatttaa
    23461 tagtgtagaa gaagctacaa aaacaattca tgattttatt cttttcttta atcatgaaag
    23521 aattacatta aaaatggctg attctgtata acaacaaaac caaccattaa ttttaattat
    23581 ttaattgtct actagactgg gtgcagttca catttctgtt cataagtccg atatcttatt
    23641 caataaaaaa gcgcgcaccc catcataagt ttgttgagtt cacgctttaa atctttattt
    23701 agttgatggg gtactctgag ctagacaata tttgtatgtg gcaaacatta tcgttgcact
    23761 catttgcttt atataaaagt agttagtgta tttatataaa ttcttatact aattcgataa
    23821 ttactgattc agcaccgtca ccgcgacgag ggccttgttt aaggatacga gtgtaaccac
    23881 cttgacgttc tgtgtaacgc tctgcgattt caccaaataa tttttgaagt gcagtttgtg
    23941 tagtttcatc ttcgtttaag atttcaacat tacgtaaagt tttagctgca ttacgacgag
    24001 aagctaaatc tcctttttta cctaaagtga ttaatttctc aacaacactg cgaacttctt
    24061 ttgcacgagc ttctgtagtt tcaatacgtt cactaataat aagtgatgta gctaagtcac
    24121 gtaacatagc tttacgttga tcagaagtac gacctaattt tctgtaaccc atgagttaac
    24181 ctcctttatc aatcttcttt tcttaatcct aatcctaaat cttctaattt gtatttaact
    24241 tcttctaaag atttacgacc taaattacgc actttcatca tgtcagcttc agatttgtca
    24301 gctaactctt gaacagaatt gattcctgcg cgttttaagc agttatatga acgtacagat
    24361 aagtctaatt cttcaataga catttctaat actttttctt tttgatcttc ttctttttca
    24421 atcatgattt cagcgttttg cgcttcatca gtaagaccaa cgaagatatt caagtgttca
    24481 gtcattattt ttgctgctaa tgaaactgat tcttgtggtg tgattgaacc attagtccaa
    24541 acatccaatg ttaatttatc aaaatcactg ctttgaccta cacgtgtatt ttcaacagta
    24601 tagttcacac gttcaacagg tgaatacaat gaatcaacag ggattacacc aattggtaaa
    24661 tcactagtat tattttgttc tgctaatgcg taacctctac ccttgttagc aactagacga
    24721 atttttaagt gaccaccttt agatactgtt gcaattttaa gctctgggtt taaaatttca
    24781 acatcactgt catgtgtaat gtcgcttgct gttacttcgc cttcatcacg tacatcaatt
    24841 tctaaagttt tatcttcttc agagtaaatt ttcaatgcta attgtttaat gttcataata
    24901 attgtagaaa catcttcaac tacattgtct actgctgaga attcatgtaa aactccctca
    24961 atttcaatgt acttaacggc tgcacctggt aatgaagata gtaggatacg acgtaaggag
    25021 tttcctagtg tagtaccgta gccacgttct agtggttcaa caacgaactt accgaattta
    25081 gcatcttcac taatttcaat tgtctcaatt ctaggttttt cgatttctat catttaaata
    25141 tcctccttat atacgtcgac ttaatttaaa atgtttgctc agtgacctgt aacaatacca
    25201 tcataaatta tacacgacga cgttttggtg gacgacaacc gttatgaggt actggagtaa
    25261 cgtctctgat cgcagttact tctaaacctg cagattgtaa tgcacgaata gctgattcac
    25321 gacctggacc aggtccttta actgttactt caactgtttt taaaccatgc tccatagctg
    25381 atttagatgc agtttcagaa gccatttgtg ctgcaaatgg tgttgatttt ttagatcctt
    25441 tgaatcctaa tgcaccagct gatgaccatg ataaagcatt accgaactca tcagtgatag
    25501 ttacaatagt gttgttgaat gttgaacgga tgtgtgctac accattttca atattctttt
    25561 tcactctacg tttacgagat acttgtttac gtgccattta aaatttgcct cctttaccta
    25621 ttattttttc ttgttagcta ccgttttaac tggtccttta cgagtacgcg cgttgttttt
    25681 cgttttttga ccacgaactg gtaaaccacg acggtgacgg ataccacggt atgatgaaat
    25741 ttccattaaa cgtttgatat ttaagttagt ttcacgacgt aagtcacctt cgactttata
    25801 accgtctaca acttcacgga tgcgacctaa ttcgtcatca gttaaatctt tcacacgagt
    25861 atcagctgat acgttagctt cttcaagaat tttttgagca gttgacgtac cgataccgta
    25921 tatataagtt aatgagataa ctacgcgttt ttcacgtgga atatctactc ctgcaatacg
    25981 tgccatatta atttacacct ctcttttatt aaccttgtct ttgtttgtgt tttggatttt
    26041 cacaaattac cattacttta cctttacgtt taatgacttt acatttttcg caaataggtt
    26101 ttactgatgg tcttactttc atttttatac ctccctatat tatggagtga cgattattta
    26161 taacgataag taattcttcc gcgtgttaaa tcgtacggag acatctcaac agttactttg
    26221 tcgccaggta gaatacgaat gtaattcatt ctgattttac cacttacgtg agctaaaatc
    26281 tcatgaccat tttctaattc tactttaaac attgcgttcg gtaaagtatc taatacagta
    26341 ccttctaatt caattacatc ttgtttagcc attgattaac ttcccccttt ttgcaatagt
    26401 aaggtaatcg tcaatagaca actttattgt tacgaatcta tcagtgatta attttataag
    26461 ttaaacaaaa attacgggaa ttaattatcg ttaattgcca ctctcatcta tctaatatga
    26521 ttaaatcatg cctcacttaa aatagaccgc taaaagttga tctattacaa atgatctaaa
    26581 atatcaatga catctttggt aacgtcgcta atatcttttg aaccatcaat atttttcaat
    26641 acaccttttt g
//
LOCUS       DAMZPP010000018        26528 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMZPP010000018 DAMZPP010000000
VERSION     DAMZPP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26528
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.018"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            59..361
                     /gene="mnhF2"
                     /locus_tag="POH73_000585"
     CDS             59..361
                     /gene="mnhF2"
                     /locus_tag="POH73_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HDI6886562.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGYVFNGNNKRNL"
     gene            336..773
                     /gene="mnhG2"
                     /locus_tag="POH73_000586"
     CDS             336..773
                     /gene="mnhG2"
                     /locus_tag="POH73_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HDI6886563.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDDHTHASILLSSNEQNSTKALQLRAKKREEYRKKWYQND"
     gene            1113..3155
                     /locus_tag="POH73_000587"
     CDS             1113..3155
                     /locus_tag="POH73_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDI6886564.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(3241..4389)
                     /locus_tag="POH73_000588"
     CDS             complement(3241..4389)
                     /locus_tag="POH73_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6886565.1"
                     /translation="MSNIKIVTLIGFFTMFIVGVSDLIVVGMLNEMSKEFHVSNALIG
                     QLVTLYAVAFSILSPLLTKVTSQINDKKLLVSCLILFIIFSMLTTVMTNYFFLCIVRI
                     FMAGIASLITVKLMSTGANIVPVEYKASVIANIYVGFSAANILGIPIGTLIASHYNWK
                     IPFYIIAIITFLCLLGIMLFIPNKSHYSINNHSKYQLVNFRGTLIMLGFLLIMMISNS
                     MLFTYIEPLVKSKGHSIWIVSLCLFFSGIAGVLGSKLGNNLTEKKGYRIAGNIIIIVY
                     IISIMSILLTGQNVILLILSIFTWNLFHWGTNPTVQYALLKFLKGDPSQILSYDISIL
                     NLGIGLGSLIGGILFSIDNQFNLSIVVAMILAMVSSILLKTAVHTEKN"
     gene            complement(4393..4891)
                     /locus_tag="POH73_000589"
                     /pseudo
     CDS             complement(4393..4891)
                     /locus_tag="POH73_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(6029..6958)
                     /locus_tag="POH73_000590"
     CDS             complement(6029..6958)
                     /locus_tag="POH73_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDI6886566.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(6955..7791)
                     /locus_tag="POH73_000591"
     CDS             complement(6955..7791)
                     /locus_tag="POH73_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDI6886567.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(7785..8528)
                     /locus_tag="POH73_000592"
     CDS             complement(7785..8528)
                     /locus_tag="POH73_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDI6886568.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLHNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            8650..9294
                     /locus_tag="POH73_000593"
     CDS             8650..9294
                     /locus_tag="POH73_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDI6886569.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            complement(9374..10126)
                     /locus_tag="POH73_000594"
     CDS             complement(9374..10126)
                     /locus_tag="POH73_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HDI6886570.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            10362..11126
                     /locus_tag="POH73_000595"
     CDS             10362..11126
                     /locus_tag="POH73_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HDI6886571.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVGEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIEL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESKVMIGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            complement(11186..11980)
                     /gene="tagH"
                     /locus_tag="POH73_000596"
     CDS             complement(11186..11980)
                     /gene="tagH"
                     /locus_tag="POH73_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HDI6886572.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDNIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            12327..13139
                     /gene="tagG"
                     /locus_tag="POH73_000597"
     CDS             12327..13139
                     /gene="tagG"
                     /locus_tag="POH73_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HDI6886573.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEIMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            13238..14341
                     /locus_tag="POH73_000598"
     CDS             13238..14341
                     /locus_tag="POH73_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6886574.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSLYQKTVLTTPKQAPYLSELSDDVNVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKATVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLALYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEHSDIDDVSSIDTSILMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDKVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            14338..15399
                     /locus_tag="POH73_000599"
     CDS             14338..15399
                     /locus_tag="POH73_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI6886575.1"
                     /translation="MRLTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMEIKMTDPHDSDAFMVINPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDVTLANTYNHEILVKAMQKATDIHLVSQMIVGDNDIDTHATSNNE
                     DFKRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFNGENWDREIYELWRQTLIQVGINRPTYKRF
                     LIQLKGRKFAHRTKSMLKR"
     gene            15462..15860
                     /gene="tagD"
                     /locus_tag="POH73_000600"
     CDS             15462..15860
                     /gene="tagD"
                     /locus_tag="POH73_000600"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HDI6886576.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(15977..17272)
                     /gene="pbp4"
                     /locus_tag="POH73_000601"
     CDS             complement(15977..17272)
                     /gene="pbp4"
                     /locus_tag="POH73_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HDI6886577.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQAANSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGACLVAGLALIVHMIINRLFRKRK"
     gene            17693..19420
                     /locus_tag="POH73_000602"
     CDS             17693..19420
                     /locus_tag="POH73_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6886578.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            19779..21008
                     /locus_tag="POH73_000603"
     CDS             19779..21008
                     /locus_tag="POH73_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDI6886579.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIGGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            21263..21355
                     /locus_tag="POH73_000604"
     CDS             21263..21355
                     /locus_tag="POH73_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886580.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            21533..22366
                     /locus_tag="POH73_000605"
     CDS             21533..22366
                     /locus_tag="POH73_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDI6886581.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAALIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            22648..23445
                     /locus_tag="POH73_000606"
     CDS             22648..23445
                     /locus_tag="POH73_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6886582.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            23481..24485
                     /locus_tag="POH73_000607"
     CDS             23481..24485
                     /locus_tag="POH73_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6886583.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGIALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLISIALIIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADMIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            24482..25498
                     /locus_tag="POH73_000608"
     CDS             24482..25498
                     /locus_tag="POH73_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6886584.1"
                     /translation="MISSNNKRRQWIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDELILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITAALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIILQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            25732..>26528
                     /gene="dhaK"
                     /locus_tag="POH73_000609"
     CDS             25732..>26528
                     /gene="dhaK"
                     /locus_tag="POH73_000609"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDI6886585.1"
                     /translation="MMKKLINKKETFLTDMIEGMLIAHPELDLIANTVIVKKAKKEQG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPIPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVETVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGHSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANEVIVMVNGMGGTPLSELNIVT"
BASE COUNT         9026 a         3815 c         4651 g         9036 t
ORIGIN      
        1 aagatagttt gttaagaagt attaagcatt atgaagactt aatattggag gtgtcgcgat
       61 gatacaaaca atcacacata ttatgattat tagttcactc attatttttg gaattgcatt
      121 aatcatctgt ttatttagat taatcaaggg acctacaaca gcagatcgtg tcgttacatt
      181 tgatacaaca agtgctgttg tcatgtctat tgtgggtgtc ttaagtgtac taatgggcac
      241 cgtttctttc ctagattcaa tcatgctcat tgccattata tcttttgtaa gctctgtatc
      301 gatttcaaga tttattggtg gggggtatgt gtttaatgga aataacaaaa gaaatcttta
      361 gtcttattgc tgctgtgatg ttgttgttag gtagttttat tgctcttatt agtgcaatag
      421 gtatcgtgaa attccaagat gttttcttaa gaagtcatgc tgcaacaaaa agttcgactt
      481 tatccgtgtt attaacttta attggtgtat taatttattt tattgtgaat acaggatttt
      541 tcagtgtgcg tttattactg tcacttgttt ttattaattt aacttcacca gtcggcatgc
      601 acttagtcgc tcgcgctgct tatcgcaacg gcgcttatat gtatcgaaaa aatgatgatc
      661 acacacatgc atcaatatta ttaagttcaa atgaacaaaa ctctacaaaa gcattacaat
      721 tacgtgctaa aaaacgagaa gagtatcgta agaaatggta tcaaaacgat tgaagtattg
      781 gctaaattgt taagcataac aatacttttg aagatttgtt ttcaaaatta atcattaaaa
      841 gagtactttg aaattggtta gtcgatttta aggtgctcat tttttgtatt ataagttaat
      901 gttgttatgt gaaagcaagt tgtttattta tacaaacaat tagtgataaa ataaaattaa
      961 ataattgcaa atttaagtac tgcaatcgta gacatatatc tatattaaag tgcttttgtg
     1021 gtcgctaact caatttatcc tctaaagtat aattacaacg agatgtgata cttatttttc
     1081 aattaaacaa tactttttag agaggtgaaa gtttggaaat atttgaaaca attcttatat
     1141 ttatagctgt tgtgatacta agttcgtttg tccatacttt catacctaaa gtacccctag
     1201 catttataca aattttcttg ggcatgttac tatttattac cccaatccct gttcaattta
     1261 attttgattc tgaattattt atggtaacaa tgattgcgcc tttgttattt gtagaaggtg
     1321 ttaatgtttc tagagtccat ttaaggaaat atattaagcc agtgatgatg atggcattag
     1381 gattagtcat tactactgtg ataggtgtcg gtttatttat tcattggatt tggccagatt
     1441 tacctattgg agcagcattt gcaattgctg ccattctttg tcctactgat gcagtagcag
     1501 tgcaagcaat tactaaagga aaggtcttgc caaaaggagc aatgacaatt cttgaaggtg
     1561 agtcattatt gaatgatgct gctggtatta tttcatttaa aatagctgtt ggagtattag
     1621 ttacaggtgc tttttcactt gttgatgctg ttcagttgtt tttaattgca tcaattggtg
     1681 gcgcagtggt tggtttactt ataggtatgg cattagtaag gttccgatta acattgatgc
     1741 gtcgaggata tgaaaacatt aatatgttta caattattca attgttaaca ccatttgtta
     1801 cgtatttaat tgctgaattg tttcacgcat caggaatcat tgcagcagta gttgcaggac
     1861 ttgtacatgg tttcgaacgt gacagaatta tgcaagtacg tacacaacta caaatgagtt
     1921 acaatcatac atggaatata ctaggttatg ttttaaatgg ctttgttttt tcaatactag
     1981 gatttttagt acctgaagtt attattaaaa ttatcaaaac agaaccgcac aatttaatct
     2041 ttttaatagg catcactatt gttgttgctt tagctgtcta tctatttaga tttgtttggg
     2101 tttatgtctt atatccttat ttttatttag ccatcagtcc attccaaaaa atgatgacta
     2161 aaaatgatga tgataatcca acgactgaga aaccaccaaa gcgaagttta tacgctttaa
     2221 ttatgacgtt atgtggtgtg catggaacaa tttctttagc aatcgcatta acgttaccgt
     2281 attttttagc aggtcatcat gcttttacgt atagaaatga tttattattt attgcatctg
     2341 gtatggttat tattagtttg gtagttgcgc aagtattatt gccattatta acgaaacctg
     2401 cacctaaaac agtaattggc aatatgtcgt ttaaagttgc tagaatttat atattagaac
     2461 aagttattga ttatctaaat caaaaatcta ctttcgaaac aagttttaaa tatggtaatg
     2521 tgattaaaga atatcatgat aaattagcat ttttaaaaac tgtagagaaa gatgatgaaa
     2581 actctaaaga attagaacgt ctacaaaaaa ttgcttttaa tgtagaaaca aagactttag
     2641 agtctttagt agatgaagga caaataacga atagtgtact tgaaaactat atgcgttatg
     2701 ctgaaagaac acaagtatat agacaagcat cattaataag aagaatgatt gtattattac
     2761 gaggtgcttt attaaaacga agagtacaaa cgagagtgaa ctcggcatct tcacttagtg
     2821 ttacggataa cttaatggaa ttaaataaaa ttaataaatt agtccattat aatgtggtta
     2881 gtcgtttgtc taaggaaaca acaaaagata atacacttga agttggaatg gtttgtgacg
     2941 gttatttaat gcgaattgaa aacttaacac catctaattt cttcaactca gcaagtgaag
     3001 atacgattac taaaattaaa ttaaatgcat tgagagaaca acgtcgcatt ttacgtgagt
     3061 tgattgatac agatgaagta tcagaaggta cagcgttaaa actaagagaa gccatcaatt
     3121 atgatgaaat ggttattgta gatagtatga cgtagttcgt aattatgcta aaagggattg
     3181 atgaaaaact gaatggcttt tcatcaatcc cttttattta ggggaattga atagatagtt
     3241 ttaatttttc tctgtatgaa cagctgtttt taataatata gatgaaacca ttgctaatat
     3301 catagcaaca acaattgata agttgaactg attatcgata gaaaataata taccaccaat
     3361 taatgatcct aatccgattc ctaaatttaa tattgaaata tcatagctca atatttgact
     3421 tggatcgcct tttaaaaatt taagtaaagc atattgaact gttggatttg ttccccaatg
     3481 aaataaattc catgtaaata tagataaaat taaaagtatg acattttgtc cagtcaataa
     3541 gatgctcata atagaaataa tatagacaat tataatgata tttccagcaa tcctatagcc
     3601 tttcttttca gttaaattat ttcctaattt tgaacctaaa acacctgcaa tacctgagaa
     3661 aaataaacac aaacttacta tccagatgct atgaccttta ctttttacta aaggctctat
     3721 atatgtaaat aacatgctat tagatatcat catgattaat aaaaagccca acatgattaa
     3781 tgtgcctctg aaatttacta attggtattt agaatgatta ttaatactat aatgtgattt
     3841 attcgggata aataacataa tacctagtag acataaaaaa gtgataatcg caatgatata
     3901 aaacggaatc ttccaattgt aatgggaagc aattaatgta cctatgggta taccaagtat
     3961 atttgctgca ctaaagccta cgtatatgtt agcgataact gatgctttgt attctactgg
     4021 aacaatattt gctccagtag acattaattt tacagtgatt aatgaagcga tgcccgccat
     4081 aaagattctg acaatacata gaaagaaata attggtcatt acagttgtaa gcatgctgaa
     4141 aataataaaa agtattaaac aggatactaa taattttttg tcattaattt gactagttac
     4201 ctttgttaag agagggctta gaattgaaaa agcaacagca tacaatgtta ccaactgacc
     4261 tattaaagca ttacttacat gaaattcttt actcatttca tttaacattc ctacaacaat
     4321 taagtctgaa acaccaacaa taaacattgt gaaaaagcca atcaaagtta caattttaat
     4381 atttgacata tcttactcct caaaattaag ttctttaaca ttattaatta taaaatttgg
     4441 attttcagtg tttaatactt caatactatg acttccccaa gttacagcac aagaatcaat
     4501 attggcattt ttagccattt taatatcaaa aacagcatct ccaacatata tagtgtcttt
     4561 gttatgtaag tcatattttt gtaatatata tataattcca tcaggagaag gtttataatt
     4621 cgttacttta tacttcaaca aatagattat caacacctaa tttagaaaga tttctttcta
     4681 atacatttgt ttttttacta gaaacaacga ataacttatg gttttgatga agtgttttga
     4741 gctgttcttg cattccgtca tatattttta aatgttttgc ttcagtttct ttataatttt
     4801 ctctaaacaa tgtcattagt tcggtgagtt ctttgtcatt tagcttaaaa tcactcatta
     4861 aattaaaaga tttttcaatc ggaataccca tataatattc tatttgttca tcgctaggta
     4921 tattcaaacc tcttttttgt ggccatgtac accaaaaaca cagggaaaac cttgagattg
     4981 aattacttct tgagaagtgt tgatttcagc ttctaactat gcatgtgagt tcttagaatt
     5041 ttttatttca ctttgcaaaa gcaacatttt atcacctcaa aatagtatcg attaatttat
     5101 attatatata aattaattaa atattgcaaa ttaattttat gtaaacttaa ttaaagggat
     5161 ttgaaggtgt tgttaacatt tgagatttaa ttgaaataag ttttaaaaat tggaggagat
     5221 agattaatcg aagtcattta aagatgaaga taagtgcatt cacaaaaaat agccacactc
     5281 atatgacatc ggatgagtgt ggcttaagga tctatggggg gaggaaacca tagatgttta
     5341 ctttgatagg ccagattaaa tatcaaagta tgcgattatt tatagcttga tgcaaaagtg
     5401 gtatgcctat taaaagttac tgcacatagc ttttaatatt ccgttcaaag gaaaggggca
     5461 tacaattgaa caatctgtaa tagtactttt aaccagctat gctaaaagtc tagtagggag
     5521 aacagttgtc caatcgcata agaacctcta acttcgttag tacgattaag aaaagctttt
     5581 tagttagtat gtaatacaat ttattgacgc gcgtgaatct cttttataag agtgtgtagg
     5641 gaatggcgtt gtataaattg tattagaaga acttctaacg catctctgtg gttaaaagag
     5701 atgaagggaa cgacagttta attaaaaaca cataaagctt ttaactttac tctttgattt
     5761 aaagagtgac aaatgtttat agtttaatta aaactgcata agaacttcta gcttttctct
     5821 ttcgctcaaa gagaagttct aataccacca tatcgtgcga tcgggaacgg tatatatatt
     5881 aataggaggg taatatatat ttaacgcacg atatgggact attagccttc gactttgtta
     5941 tgttgatgtg tggcctaaaa tattggagat accaatattt taggttgcat caacatcaat
     6001 tcatcttact tcattaaaac acagcgtgtt atttcatgct tccgtgtaca gtttcaatat
     6061 ttgatttcat cattttgtag taagagtcac ctttagtgcc ttctttaccg attgaatctg
     6121 tgtacacttc accaaagata tctttcttcg tttcttcaga taaactttcc attgctttct
     6181 tatcaacact tgtttctact aataagtgtt ttaatttgtg ctttttaaca aactcaatag
     6241 cttgtctcat ttgttctggt gtaccttgtt tttcagtgtt aatttcccaa atataacctg
     6301 gtgtaatacc gtattgtttt gagaagtact tgaaggcacc ttcacttgta atcatggcac
     6361 gttgttcttt tggaatgtca ttaaatttgt ctttactgtc gttatttaat ttttccaatt
     6421 gagcaatgta tttgttacct tgcttttcat aatctgcttt atgttttttg tcgttatcga
     6481 taaatgtttg ttgaattgtt tttacgtatt taataccgtt atctaaactt aaccatgcgt
     6541 gtggatcttg tttatctttg ttgccttctt caccgtttaa atagataggt ttaacatctt
     6601 ttgatactgc gataactttt ttatctttta atgatttacc agcctgttct aaggcttttt
     6661 caaaccaacc gttaccagtc tctaaattta atccgttgta taaaataacg tcagcgtcag
     6721 ttaacttttt aatatcttta ggtttaactt catattcatg aggatcttga ccaacaggta
     6781 caatactatg aatatcgacg ttgtctccac caacattttt agccatatca tataaaattg
     6841 aattcgtcgt tactactttt agtttgccat ttgacttatc actgctttgt ttaccaccag
     6901 taccacatgc agcaactaaa agtaataagg ctaataataa aggtactaat tttttcatgt
     6961 taaacttcct cgtttctttc tattcgtaaa ttttgtgaaa aataatgtga tgatataaat
     7021 tacaaacgta caaagtacga ttgttgcacc actaggaatg ttgtaaatat agctgtaata
     7081 aagtccgaca attgaactta tgacacttat taaacttgct ataatcatca ttgagtatag
     7141 ttttttacta attaaaaatg ctgtagatgc aggtgtaatt aataatgcaa ctacaagaat
     7201 aatacctacc gtttgaatac ttgctactgt tactaatgag agtaacaaca tcacaaagta
     7261 atgtaataac gtcgtattta gaccactcat tctactaaaa gttggatcga atgtagaaat
     7321 cattaatgga cgatagaaaa taatgattag aataaggacg attgaaccaa tcactatagt
     7381 tgttaaaaat gcactatttg tgattgccag taaattacca aacagaatat ggtacaaatc
     7441 tgtcgtagtg tttattaagc taataataat aatccccgaa gctaagaaag cggtaaaact
     7501 aattccaata gcggcgtcag gtttcgtttt actactagat gtgatataac cgataaaaat
     7561 acttgcgatc ataccagtta taagtgcgcc tacaaacatt ggaataccaa ataagaatga
     7621 tagggcaaca ccaggtaata ctgcgtgact cattgcatct cccattaatg aaagaccacg
     7681 taatacaatt aaactaccaa ctgtaccaca aactatccct acaataattg aagttatcaa
     7741 tgctcgattc aagaattgat atgtaaataa atgttcgaca aactctaaca tgttatattg
     7801 ctcctttgac tagggtcact acagtcagtg ctactcataa atgtttcgtt taagcgagtg
     7861 acactcatag cctcttcact atcaccaaag tatcgtaatg tttgatttaa tagaataatg
     7921 cgatcaaagt attgctttgc ttttgataaa tcatggtgga tgataagaat aagttttcct
     7981 tgttgtttta agttctcgat ttttgtcatg attaattttt cactactaaa atcaattccg
     8041 acaaacggct catctagaaa ataaacttca ctttcggaca tcaatgctct cgctactagc
     8101 acacgttgta attgtccacc acttaattct gaaatttgtc gatgacgtaa agattctaat
     8161 tctaaatcgc ttaataactg tttgagttta tcccttgctg atttattagg tcgtctaaac
     8221 catccaattt ctttgtagca acctgataaa atcacttgtt ccacacttat aggaaaatct
     8281 aaatcaatat gtgctttttg tggaatatat gtaatatgtt gcagttgttg ttgtataggt
     8341 ttgttatgta acaatttagt accggtagca ttaaattcac caattaaaga cttgataagg
     8401 gaagatttac cagcaccatt cgggcccatg ataccaatta tttcgccgcg tactggtatc
     8461 gataaggaaa tgtttttaag tacatgctta ttacctaaaa acagatttaa atcttttgtt
     8521 tctaacaaac gtttatacct cctaattaaa agtttaggct aacctaatta attgtataat
     8581 aaactgagaa tatttatcat gtcaagtaaa ttcgtgatat aatatagaca atgtatgtga
     8641 ggtgaaagta tgttaactga agaaaaagag gactatttaa aggcaatcct tacgaataat
     8701 ggcgataaaa attttgtgac aaataaaatc ttatctcaat ttttaaatat taagcctcca
     8761 tctgtaagtg aaatggtagg acgtctcgaa aaagcaggct atgttgaaac aaaaccgtac
     8821 aaaggtgtta gattaacaga ggatggttta acgcatacgc ttgatatcat taagagacat
     8881 cgactattag aattattttt aatagaaata ttgaaatata attgggaaga agtacatcaa
     8941 gaagcagaaa ttttagaaca tcgaatttca gatttatttg ttgaaaggct ggatagcttg
     9001 ttaaatttcc cagaaacttg cccgcacggc ggtgtgattc ctagaaataa tgaatataaa
     9061 gagaaatata taacaacgat tttgaattat gaacctggtg atatcgttac aatcaaacgt
     9121 gtgagagata agactgattt gctaatatat ttgtctagta aagatatctc tattggtaat
     9181 gaagtggaaa ttgtatcgaa agatgaaatg aataaagtaa ttatcatcaa acgtaatgat
     9241 aatgtaatta ttgtcagtta cgaaaatgca atgaacatgt ttgctgaaaa ataaaataaa
     9301 gaagccataa agatatccat gattgtactg ataaagacat atggataatt gctttaggct
     9361 tcttttttat tagttaattt atcaagtgag tatatttgag taaaatattc actgcataaa
     9421 gattgaagat aatccagatt gtactataaa tgaagatagg tacatgactg agttctttaa
     9481 gtgcactacc atcccactgt ggactcggac gctggaaagt caatttagca atcgtccaac
     9541 tagattgtag aacttcgcct aataatacac ctaaaatata ttgataactc attgtgacaa
     9601 gtagttgaat ttctactata ttttcatctt ttaatataaa atacaacatg atagaaatta
     9661 aagttataac aacaatgggt gagccttttc tagatgttaa aattaaaaaa taaataaata
     9721 tcaataaata ggtaaatata aagaaactag gtatctgata atggctcgac gctagaccaa
     9781 tcaataacat aataggtggc ataaaataac caccaatcgt tgtaagccat tggcctgcta
     9841 gatgtctaga ttgtgtaatt gcgaatcctt gttgtaatgt ctgttgtcgc tctcgtgggc
     9901 ttgttacaat gactaaatct tttgcacggc caccagcgag tttattaaac agtacatgac
     9961 caaattcatg tgttaaaaca gggatatagt ttaaaatgac atctaaatag ttcaaaacag
    10021 gcttatgtct atattgatga atagcaatat aacaagctgc aacaataacg ataatgtata
    10081 tattaagttg aattgtcgta ttaaaaaagt tggataaata attcattgtt aacctcatat
    10141 aagatattaa tttaaagttt gcttatcact tattataaat gatattggca tcaatagcgt
    10201 tagactttag acttacctta gttaaactaa ttttaatttt tgaaaaggtg aatatgtgtt
    10261 aaaataaagc aaaatcattt cgatataaat aggatgaata taaatactgt taatattgat
    10321 tacactaaca taataatgaa ataagatagg agattcctgt tatgactgtt gaagaaagat
    10381 ccaatacagc caaagttgac attttagggg tcgattttga taatacaaca atgttgcaaa
    10441 tggttgaaaa tattaaaacc ttttttgcaa atcaatcaac gaataatctt tttatagtaa
    10501 cagccaaccc tgaaatagtg aattacgcga cgacacatca agcgtattta gagttaataa
    10561 atcaagcgag ctatattgtt gctgatggga caggagtagt caaagcttcg catcgtttaa
    10621 agcaacctct agcgcatcgt atacctggta ttgagttgat ggatgaatgt ttgaaaattg
    10681 ctcatgtaaa tcatcaaaaa gtatttttgc taggggcaac taatgaagtt ggagaagcgg
    10741 cacaatatgc attgcaacaa agatatccaa acatatcgtt tgcacatcat catggttata
    10801 ttgatttaga agatgagaca gtagtgaaac gaattgaact gtttaaacct gattacatat
    10861 ttgtaggtat gggattccct aaacaagaag aatggattat gacacatgaa aatcaatttg
    10921 aatctaaagt gatgataggc gtaggtggtt ctcttgaagt atttgctggg gctaaaaaga
    10981 gagcgcctta tatctttaga aaattaaaca ttgaatggat atatagagca ttaatagatt
    11041 ggaaacgtat tggtagatta aagagtattc ctatatttat gtataaaata gccaaagcaa
    11101 aaagaaaaat aaaaaaggca aaatagtcat gatgacaaaa taaaaccgag gaaatcctta
    11161 aatggagatt ctcggttttt tcggtttatt taataacgaa gcgggactca tcgagtttgt
    11221 ttctaaattc tttttgttcg gctttggatt tctttttaaa atcgtttagg aaagcttcat
    11281 atttaggtaa tacatcatca agttcaccgt aatcttttaa ctttccgcct tcaatccaag
    11341 caatcttagt acaaaattgt ctcacttggc ctaagttatg actaacgaaa aagatggttt
    11401 tgttttgctc tttaaactcg taaatattat ctaaacattt ttgtgcaaaa gtttggtcac
    11461 ctacagataa agcttcgtca atgactaaga tatctggatt aactgtgata ttaattgaaa
    11521 aaccaagttt tgcacgcata ccacttgaat actttttaac tggttgatag ataaactcac
    11581 caagttcact aaattcaata atcttaggtg tcatcgcttt aatttcttta cgcttaaagc
    11641 ccatacataa cattttaaat tcgatatttt caatccctgt aagttgtcca ctcaagccag
    11701 cactaattgc gataacgctg acttcaccat tacgatccac ttttccaaca gtaggcgaca
    11761 atgaaccccc aatgatattg ctcaacgttg atttgccgga accattgatg ccaacaagcc
    11821 ctatgacgtc accttcatat gcttttaaac taatgtcatc taaagcgaaa aatgttttgt
    11881 ttttatgttt gggaatgagc gcatctttca tacgttcttt atttgtacga taaatacgat
    11941 attcttttgt tacattttta atgtttaccg aaacgttcat ttgtagacct tccttattca
    12001 catttatcta gattataata tactactcaa cagttgttaa attttaaaac ctgttgtaaa
    12061 gtgtatagaa gattttgtta ttatcagagt tggtgttttg acacaaaatg ttaatcatca
    12121 atgataacaa tgatatttaa aaactaaact tatttcaact tacatgattg tatactataa
    12181 tgtatttgta ataaactaat attttaaaga actagacaat aattttgata gcatccatgt
    12241 atagtgatag tatttacaac aattattata atactattta gttaagtaga gaaatagtta
    12301 aacatttgaa agtgtggttt aatggaatgt cagcaatagg aacagttttt aaagaacatg
    12361 taaagaactt ttatttaatt caaagactgg ctcagtttca agttaaaatt atcaatcata
    12421 gtaactattt aggtgtggca tgggaattaa ttaaccctgt tatgcaaatt atggtttact
    12481 ggatggtttt tggattagga ataagaagta atgcaccaat tcatggtgta ccttttgttt
    12541 attggttatt ggttggtatc agtatgtggt tcttcatcaa ccaaggtatt ttagaaggta
    12601 ctaaagcaat tacacaaaag tttaatcaag tatcgaaaat gaacttcccg ttatcgataa
    12661 taccgacata tattgtgaca agtagatttt atggacattt aggcttactt ttacttgtga
    12721 taattgcatg tatgtttacg ggtatttatc catcaataca tatcattcaa ttattgatat
    12781 atgtaccgtt ttgttttttc ttaactgcct cggtgacgtt attaacatca acactcggtg
    12841 tgttagttag agatacacaa atgttaatgc aagcaatatt aagaatatta ttttactttt
    12901 caccaatttt gtggctacca aagaaccatg gtatcagtgg tttaattcat gaaataatga
    12961 aatataatcc agtttacttt attgctgaat cataccgtgc agcaatttta tatcacgaat
    13021 ggtatttcat ggatcattgg aaattaatgt tatacaattt cggtattgtt gccattttct
    13081 ttgcaattgg tgcgtactta cacatgaaat atagagatca atttgcagac ttcttgtaat
    13141 atatttatat gacgaaaccc cgctaaccat taataaatgg aagtggggtt catttttgtt
    13201 tataatttaa gtaaataaca tattaagttg gtgtattatg aacgttttaa taaagaaatt
    13261 ttatcatttg gtagttcgaa tactttctaa aatgattacg cctcaagtga ttgataaacc
    13321 gcatatcgta tttatgatga cttttccaga agatattaag cctatcatca aagcattaaa
    13381 taattcgttg tatcagaaaa cggttttaac aacaccaaaa caagcgcctt atttatctga
    13441 acttagcgac gatgttaatg tgatagaaat gactaatcga acactggtaa aacaaattaa
    13501 ggctttgaaa agcgcgcaga tgattattat cgataattat tacctattac taggtggata
    13561 taataagact tctaatcaac acattgttca aacgtggcat gcaagtggtg cgttaaaaaa
    13621 ctttggctta acagatcatc aagtcgatgt gtctgacaag gcaacggttc agcagtaccg
    13681 taaagtttat caagcgacag atttttactt agtgggttgt gaacaaatgt cacaatgttt
    13741 taaacagtct ttaggtgcaa cagaagagca aatgttgtat tttgggcttc cgagaattaa
    13801 taaatattac acagctgata gagcaacggt taaggcagag ttaaaggata aatatggaat
    13861 tacaaataag ttggcattat atgtaccaac atatagagaa gataaagcag ataatagggc
    13921 tattgataaa gcttattttg aaaaatgttt accaggatat acactgatta ataaactaca
    13981 tccatcaatt gaacattcag acattgatga tgtatcttcg atcgatacgt ctatattaat
    14041 gttaatgtca gatataatta ttagcgacta tagttcgctg ccaatagaag ctagcttgtt
    14101 agatattcca actatatttt atgtgtatga tgaaggaaca tatgataagg tgagaggcct
    14161 gaatcaattt tacaaagcaa taccggatag ctacaaagtg tatactgaag aggatttaat
    14221 aatgacgata caagaaaaag aacatctatt aagtccatta tttaaagatt ggcataagta
    14281 taatactgat aaaagtttac atcagctcac agaatatata gataagatgg tgacaaaatg
    14341 aggcttacga taatcatacc tacatgtaat aatgaggcaa caattcgaca attgttaatc
    14401 tctattgaga gtaaagaaca ctatagaatc ctttgtattg atggtggttc tactgatcaa
    14461 acaattccta tgattgaacg gttacaaaga gaactcaagc atatttcatt aatacaatta
    14521 caaaatgctt cgatagctac gtgtattaat aaaggtttga tggagatcaa aatgacagat
    14581 ccacatgata gtgacgcatt tatggtcata aatccaacat caatcgtatt gccaggtaaa
    14641 ttagataggc taactgcagc attcaaaaat aatgataata ttgatatggt aatagggcag
    14701 cgagcttaca attaccatgg tgaatggaaa ttgaaaagtg ctgatgagtt tattaaagac
    14761 aatcgaatcg ttacattaac ggaacaacca gatttgttat caatgatgtc ttttgacgga
    14821 aagttattca gtgctaaatt tgctgaatta cagtgtgacg taactttagc taacacttac
    14881 aatcacgaaa tacttgtcaa agcgatgcaa aaagctacag atatacattt agtttcacag
    14941 atgattgtcg gagataacga tatagataca catgctacaa gtaacaatga agattttaaa
    15001 agatatatca cagaaattat gaaaataaga caacgagtca tggaaatgtt actattacct
    15061 gaacaaaggc tattatatag tgatatggtt gatcgtattt tattcaataa ttcattaaaa
    15121 tattatatga atgaacaccc agcagtaacg cacacgacaa ttcaacttgt aaaagactat
    15181 attatgtcta tgcagcattc tgattatgta tcacaaaata tgtttgacat tataaataca
    15241 gttgaattta atggtgagaa ttgggataga gaaatatacg aattgtggcg acaaacatta
    15301 attcaagtgg gcattaatag gccgacatat aaaagattct tgattcaact taaagggaga
    15361 aagtttgcac atcgaacaaa atcaatgtta aaacgataat gtgtacattg atgaccataa
    15421 actgcaatcc tatgatgtga caatatgagg aggataactt aatgaaacgt gtaataacat
    15481 atggcacata tgacttactt cactatggtc atatcgaatt gcttcgtcgt gcaagagaga
    15541 tgggcgatta tttaatagta gcattatcaa ctgatgaatt taatcaaatt aaacataaaa
    15601 aatcttatta tgattatgaa caacgaaaaa tgatgcttga atcaatacgc tatgtcgatt
    15661 tagtcattcc agaaaagggc tggggacaaa aagaagacga tgtcgaaaaa tttgatgtag
    15721 atgtttttgt tatgggacat gactgggaag gtgaattcga cttcttaaag gataaatgtg
    15781 aagtcattta tttaaaacgt acagaaggca tttcgacgac taaaatcaaa caagaattat
    15841 atggtaaaga tgctaaataa attatataga actatcgata ctaaacgata aattaacgta
    15901 ggttattata aaataaatat aaaacggaca agtttcgcag ctttataatg tgcaacttgt
    15961 ccgtttttag tatgttttat tttctttttc taaataaacg attgattatc atatgaacaa
    16021 ttagtgctaa tccagcgact aggcatgcac caccaatgat agtgaataat ggatgttctt
    16081 cccacatact tttagcaaca gtatttgcct tttggataat tggctgatga acttctacag
    16141 ttggaggtcc ataatcttta ttaataaatt ctcttggata gtccgcgtgt actttaccat
    16201 cttcgactac aagtttataa tcttttttac taaaatcact tggtaaaaca tcgtaaagat
    16261 cattttcaac ataatatttc ttaccattta tcctttgctc acctttagac aatattttta
    16321 catatttata ctgatcaaat gaacgttcca ttaatgcatt ccccatcata ttacgttgct
    16381 tctcgccacc aaggttttta tagtctcctg cacccatgat aacttgatta attctaaatt
    16441 tacctcgttt agtagtaatc gtatggttgt aatttgctgt atcacttgat ccagttttta
    16501 aaccatctgt accaggcaaa ctcatttttg caccttccaa tgaaaagttg aatgtgtaat
    16561 acgtaactgc atgcgttgtt ggtgctaact gctttgtaaa gtctaatatt ttaggtgtct
    16621 ctttaatcac gtgtaaatct aaaatggcgt agtctctagc agtcgttaca gtacgttctt
    16681 ggtctttata ttttgttggt gcaaatgtac gtaatcttga attttcagca cccgttggat
    16741 tgacgaaatg tgtatttttc attccgatag ctttagcttt gttattcatt aaatcaacga
    16801 aatcgctggt gttttttgat accttcttag ctaaaattaa tgccgcggca ttactagaat
    16861 tagatactgt aatttgtaat aggtctgcga ttgtccatac ttgtccagga tatagtttcg
    16921 tattactcaa ctcaggtagt gtagacataa tatattcttt gttcgtcatt gtgactgtgt
    16981 catcaagtga aagctgcccc ttatttacag cttccaatgt taagtacatt gtcattaatt
    17041 tagtcataga cgctggattc cacttagtat cgatattgta ttgatacagt aattgtccag
    17101 tttgactaac attaacagca ctcgtcggtt cgtatgcagc cgacaaacct gcataaccat
    17161 attgatttgc tgcttgtaca ggggttacgt cactgttagc agcttgtgca tatggtgtca
    17221 taatacttaa tgttaaacat aaaatgatga taatagatat taaatttttc ataaagcgtt
    17281 aatcttccct tttccaattc ttaaatattc cctaaaagca atggtcattc ctacttacgg
    17341 aaatcattgc taattcactt caccttaatt aaattgttga aaattaagtt tcttgcggtt
    17401 tatttgaaaa ataatgcaaa tatattacgt acgtagctaa agatgttata atgtttgtac
    17461 gaagagcaaa cttactcaaa agtgattaat tatcatgttt taatataaaa actttgagaa
    17521 gttattacaa aaaatgcaat agaaatattc tatcatataa atgttatgag cggtattttg
    17581 gggcaatact ttatttgatt tttaaagttt tgttgggaga aaatatatga tagaaatgca
    17641 tgtatctatc taaatgaatt aactataaat ttcaaacaga agaggtaaaa ctatgaaacg
    17701 agaaaatcca ttgtttttct tatttaaaaa actatcatgg ccagtgggtc ttatcgttgc
    17761 agctatcact atttcatcac tagggagctt aagtggacta ttagtgccac tgtttactgg
    17821 acgaattgta gataaatttt ccgtgagtca tatcaattgg aatctaatcg cattatttgg
    17881 tggtatcttt gtcatcaatg ctttattaag cggattaggt ttatatttat taagtaaaat
    17941 tggtgaaaaa attatttatg cgatacgctc agttttatgg gagcatatca tacaattaaa
    18001 aatgccattc tttgacaaaa atgaaagtgg tcaattaatg agtcgattaa ctgacgatac
    18061 gaaagtgata aatgaattta tttcacaaaa gctacctaac ttattaccat caatcgttac
    18121 attagttggg tcactaatca tgttatttat tttagattgg aaaatgacat tattaacatt
    18181 tataacgata ccgatatttg ttttaattat gattcctcta ggacgtatta tgcaaaagat
    18241 atcgacaagt acacaatctg aaattgcaaa ctttagtggt ttgttagggc gtgtcctaac
    18301 tgaaatgcgt cttgttaaaa tatcaaatac agagcgactt gaattagata atgcacataa
    18361 aaatttgaat gaaatatata aattaggttt aaaacaggct aaaattgcgg cagttgtaca
    18421 accaatttca ggtattgtta tgttgctaac aattgcaatt attttaggtt ttggtgcatt
    18481 agaaattgcg actggtgcaa tcactgcagg tacattaatt gcaatgatat tttatgttat
    18541 tcaattatcg atgcctttaa tcaatctttc aacgttagtt acagattata aaaaggcagt
    18601 cggtgcaagt agtagaatat acgaaatcat gcaagaacct attgaaccga cagaagctct
    18661 tgaagattct gaaaatgtat taattgatga cggtgtgttg tcatttgaac atgtagactt
    18721 taaatatgat gtgaagaaaa tattagatga tgtgtcgttc caaatcccac aagggcaagt
    18781 gagtgctttt gtaggtcctt ctggatctgg taaaagtacg atatttaatc tgatagaacg
    18841 tatgtatgaa attgagtcag gtgatattaa atatggcctt gaaagtgtct atgatatccc
    18901 gttatctaag tggcgacgca aaattggata tgttatgcaa tcaaattcga tgatgagtgg
    18961 tacaattaga gacaatattt tatacggaat taatcgccat gtttcagatg aagaacttat
    19021 taattatgct aaattagcga actgtcatga ttttatcatg caatttgatg aaggatatga
    19081 cacgcttgta ggtgaacgag gattgaaact gtctggcgga caacgtcaac gtattgatat
    19141 tgctagaagt tttgttaaaa atcctgatat tttgttactt gatgaagcaa cagctaatct
    19201 cgatagtgaa agtgaattga aaattcaaga agctttagaa acattgatgg aaggtagaac
    19261 aacgattgtc attgcgcatc gtttgtctac aattaaaaaa gccggtcaaa ttatattctt
    19321 agacaaagga caggtaacag gtaaaggtac gcattcagaa ctgatggcat cacatgcgaa
    19381 gtataaaaac tttgtagtgt ctcaaaaatt aacagattaa atttatatat ataagtaagc
    19441 ttggagcaag tacagatata ccatcgagaa aattaaaatg cggcacattg atggatatag
    19501 atgttaataa attgcttcaa gcttttgtct attttaaatc atgtgagaag ttacgacata
    19561 ataattctta aattaatgaa atcgatattt taagaaaaaa atgctcatgg tataatacaa
    19621 gttataagca aacatacata tattaaatac tgtagccacg agtcataatt cttcatattt
    19681 tacatagcaa tttaactgat tttagagtcc acggtacaga agtttgatat ttcaatgttt
    19741 ctaaattttt aaaaaattaa atcataggtg ggtgccaaat gtttttatta atcaacatta
    19801 ttggtctaat tgtatttctt ggtattgcgg tattattttc aagagatcgc aaaaatatcc
    19861 aatggcaatc aattgggatc ttagttgttt taaacctgtt tttagcatgg ttctttattt
    19921 attttgattg ggggcaaaaa gcagtaagag gagcagccaa tggtatcgct tgggtagttc
    19981 agtcagcgca tgctggtaca ggttttgcat ttgcaagttt gacaaatgtt aaaatgatgg
    20041 atatggctgt tgcagcctta ttcccaattt tattaatagt gccattattt gatatcttaa
    20101 tgtactttaa tattttaccg aaaattattg gaggtattgg ttggttacta gctaaagtaa
    20161 caagacaacc taaattcgag tcattctttg ggattgaaat gatgttttta ggaaatactg
    20221 aagcattagc cgtatcaagt gagcaactaa aacgtatgaa tgaaatgcgt gtattaacaa
    20281 tcgcaatgat gtcaatgagc tctgtatcgg gagctattgt aggtgcgtat gtacaaatgg
    20341 taccaggaga actggtacta acggcaattc cactaaatat cgttaacgcg attattgtgt
    20401 catgcttgtt gaatccagta agtgttgaag agaaagaaga tattatttac agtcttaaaa
    20461 acaatgaggt tgaacgtcaa ccattcttct cattccttgg agattctgta ttagcagcag
    20521 gtaaattagt attaatcatc atcgcatttg ttattagttt tgtagcatta gctgatctat
    20581 ttgatcgttt tatcaatttg attacaggat tgataggagg atggataggc ataaaaggta
    20641 gtttcggttt aaaccaaatt ttaggtgtgt ttatgtatcc atttgcgcta ttactcggtt
    20701 taccttatga tgaagcgtgg ttggtagcac aacaaatggc taagaaaatt gttacaaatg
    20761 aatttgttgt tatgggtgaa atttctaaag atattgcatc ttatacacca caccatcgtg
    20821 cggttattac aacattctta atttcatttg caaacttctc aacgattggt atgattatcg
    20881 gtacattgaa aggaattgta gataaaaaga catcagactt tgtatctaaa tatgtgccta
    20941 tgatgctatt atcaggtatc ctagtctcat tattaacagc agctttcgtt ggtttatttg
    21001 catggtaata tgtcgaagag tgactacgat aatacatttt aactaaaaaa gatgtccagg
    21061 catgtcgtct attgatatag gtgagatgct tggacttttt tattattgat ataaagatat
    21121 ttaaatattt ttaaagttat cgaaattgat gcaatataaa aaccaagtgc acatggtaat
    21181 acacttggct tttatgggaa atgaatatta ttgtacatat gacagtaagg actaggtaca
    21241 gtcatagtac ttcgagcaaa atttgttttg ttattataaa caacacaaag gagataactt
    21301 ctctattgaa gaagttaaaa acattatagc agacaatgaa atgaaagtaa attaaaaatt
    21361 cagaatattt ttaattatta tattgtgagt gatatttatt agggaaagct attcttcata
    21421 taaattagtt aaatagtaaa tctttgttag aactctttcc atagagttgt gacagtattt
    21481 ttgttgtgct actattttta tagtctaata aatatataaa ggggatggtt tcgtgaataa
    21541 aacggttaaa gatttaatac tagttgtctt aggttcattt atctttgctg caggtgtaaa
    21601 tgcatttatt atttctggta acttaggtga aggtggggtt acaggtttag caattatttt
    21661 atattatgcg tttcatattt caccagccat cactaacttc ttggtcaacg cagcattgat
    21721 tgccataggt tataaatttt tgagtaagag aagtatgtac ttaactattc ttgtaacaat
    21781 tcttatttca atatttctga gtttaacaga atcatggcaa gtagaaactg gaaacagcat
    21841 tgtgaatgcc atttttggtg gtgtaagcgt tggactagga atcggagtaa ttatccttgc
    21901 aggcggtaca acagcaggta caacaatttt ggcgagaatt gcaacgaaat acctcgatgt
    21961 aagcacgcca tatgctttgc ttttctttga tatgatcgtt gttgcaattt cacttacagt
    22021 tattccactt gataaagtat tagtaacagt aatatcactt tatataggaa caaaagtgat
    22081 ggaatatgtc atagaaggtt taaacactaa aaaagctatg acgattattt caactaatcc
    22141 cgacaaactt gccaaagcaa tagacgagca aattggaaga ggtttaacca ttttaaacgg
    22201 acatggctat tatacacgtg aagaaaaaga tgtcttatac gttgttattt ctaaaacaca
    22261 agtatcaaaa gcaaagcgat taattaaaca aatcgataaa gatgcattcc tcgtaattca
    22321 tgatgtaaga gatgtctatg gtaatggttt tcttgcagat gaataaataa atggtaagag
    22381 cacacatact taaatagaag tccgcggaca agtttttgaa ttatgaagac ttatctgtgg
    22441 acgtttttta ttttataaaa gtaatataca agacatgaca aatcgagcta tccaatttaa
    22501 aaagtaatgt tagtcaataa gattgaaaaa tgttataatg attttcatga taatcattat
    22561 caattgggat gcctttgaaa attgataatt taaaaataga aattattttt tataaacaga
    22621 aagaatttta ttgaaagtag ggaaattatg aatcgtttgc atggacaaca agttaaaatt
    22681 ggttacgggg ataacacgat tataaataaa ttagatgttg aaataccaga tggcaaagtg
    22741 acgtcaatca ttggtcctaa cggctgcggg aaatctactt tgctaaaggc attgtcacgt
    22801 ttattggcag ttaaagaagg cgaagtattt ttagatggtg aaaatattca tacacaatct
    22861 acgaaagaga ttgcaaaaaa aatagctatt ttacctcaat cacctgaagt agcagatggc
    22921 ttaactgttg gagaattagt ttcatatggt cgttttccac atcaaaaagg atttggtaga
    22981 ttaactgctg aggataagaa agaaattgat tgggcaatgg aagttacagg aactgataca
    23041 ttccgacacc gctcaatcaa tgatttaagt ggtggtcaaa gacaacgtgt ttggattgca
    23101 atggcattag cacaaagaac tgatattatc tttttagacg aaccaaccac atatttagat
    23161 atctgtcatc aattagaaat actagaatta gttcagaagc taaatcagga acaaggttgt
    23221 acaattgtca tggttcttca tgatatcaac caagcgattc gtttctcaga tcatcttatt
    23281 gcgatgaaag aaggcgatat catcgctaca ggttcaacag aagacgtatt aacacaggaa
    23341 attttagaaa aagttttcaa tattgatgtt gttttaagta aagatcctaa aactggaaaa
    23401 cctttactgg taacttatga cttatgtcgc agagcttatt cttaattaag taagttaata
    23461 tgataaaaag gacaattaac atgacaaata gagagaatcc aacgccattg aagtttttat
    23521 cctatattat aggtttaagt atgatactac taatcacact ttttatttct acattaatag
    23581 gtgacgccaa aattcaagcc tctacaatta tagaggctat ttttaattat aatcctagca
    23641 atcaacagca aaacatcatc aatgagatta ggattccgag aaatatagca gcagtaattg
    23701 tgggtatagc gcttgcagtt tctggtgcga ttatacaagg tgttactcgt aatggtcttg
    23761 ctgatccggc gctcataggt ttaaattcag gtgcttcatt tgctttagca ttaacatatg
    23821 cagttttacc aaacacttca tttttaatat tgatgtttgc tggattttta ggtgctattc
    23881 taggaggtgc tattgtatta atgataggcc gatctagacg tgatggattt aatccgatgc
    23941 gtattatttt agcgggtgca gcagtaagtg ctatgttaac agcgctaagt caaggtattg
    24001 cattagcttt taggttaaat caaacagtaa cattttggac tgctggaggc gtttcaggca
    24061 caacatggtc acaccttaag tgggcaattc cattaattag tattgcgtta atcattatat
    24121 taacaattag taaacaactt accattttaa atcttggtga atcattagct aaaggtttag
    24181 gtcaaaatgt aacaatgatc agaggcatat gtttaattat tgctatgatt ctagcaggta
    24241 tagcagttgc tatcgctgga caagttgcat ttgtaggatt gatggtgcct catatagcaa
    24301 ggtttttaat tggaactgat tatgctaaaa ttctaccatt aacagccttg ttaggtggga
    24361 tactcgtgct tgttgctgat atgatagcac gatatttagg agaagcgcct gttggtgcaa
    24421 tcatttcatt tatcggtgtt ccttactttt tatatttagt taaaaaagga ggacgctcaa
    24481 tatgattagt tcaaataata aacgcagaca atggatagca ctggctgttt ttagcattct
    24541 actatttcta ggttgtactt ggagtattac ctcaggtgaa tacaacatac ctgtagaaag
    24601 atttttcaaa actttaattg gacaaggtga tgccattgat gaattaatct tattagattt
    24661 cagattacct cggatgatga ttactatttt ggcaggtgca gcgcttagta ttagtggtgc
    24721 aatagtgcaa agtgtcacga aaaatccaat agctgaacca ggtatattag gtattaacgc
    24781 aggtggcgga tttgcaatcg cattatttat tgcaattggt aaaattaatg ctgacaactt
    24841 tgtttatgta ctgccgttaa taagtatact aggtggtatc accgctgcat tgattatttt
    24901 tattttcagt tttaataaaa atgaaggtgt tacacctgcg agtatggtat taataggtgt
    24961 aggtttacaa acagcattat atggtggctc aattacaatt atgtcaaaat ttgatgataa
    25021 gcaatctgat ttcatcgctg cttggtttgc aggtaatatt tggggtgacg aatggccatt
    25081 tgtcattgca tttttaccgt gggtgttgat tattattcct tacttactat ttaaatcgaa
    25141 tacactaaat attattcata cgggtgataa tattgcacga ggtctaggtg taaggttaag
    25201 cagagaacgt ttaatattat tctttatcgc agtgatgtta tcatctgctg ctgtagcagt
    25261 agcaggttca atttcgttta tcggattaat gggcccgcat attgccaaac gtatcgttgg
    25321 accacgtcac cagttgtttt taccaattgc cattttagta ggggcatgtt tacttgttat
    25381 agctgataca attggcaaaa ttatattaca accaggtggg gttccagcag gtatagtcgt
    25441 agcaattatt ggcgcaccgt atttcttata tttaatgtac aaaacgaaaa atgtatagtg
    25501 tcaaaggaca caacttattg ctatgaaagg cactttatta tatggctttt catagcattt
    25561 tttatttaat gagtcactca agactattta cattttcaat aatgaaccat taagttatca
    25621 agactgtctt atcataaata tattttataa cggtatcagg ttaattcttt atgttagcgc
    25681 attcatttat tctgttttat actatgactg acaataccaa ggaggtacaa catgatgaaa
    25741 aagttaatta ataagaaaga aacattttta actgatatga tagagggcat gttaattgcg
    25801 catccagagt tagatctgat tgctaataca gttatagtaa aaaaagctaa aaaagaacaa
    25861 ggtgtagcaa tagtctctgg aggtggaagc ggacatgaac ctgcgcatgc tggatttgta
    25921 gctgaaggta tgctagatgc agcggtttgt ggcgaagtat ttacatcacc tataccagat
    25981 aaaatattag aagctattaa agcagtagat actggtgatg gtgtattact agttgtgaaa
    26041 aactatgcag gtgacgtgat gaattttgaa atggcacaag agcttgcaga aatggaaggt
    26101 ataaatgttg aaacagttat tgttcgtgac gacattgctg tgacaaatga agtacaacgt
    26161 cgtggtgttg caggaacagt gtttgttcat aagcttgccg gttatcttgc tgaaaaaggt
    26221 cattcattaa cagagattaa atcgcgtgta gaagcgttgt tacctgaaat taaaagtatt
    26281 ggtatggcaa ttgagccacc gcttgttcca actactggaa aatatggctt tgatattgaa
    26341 gacgacaaaa tggaaatcgg tattggtata catggtgaaa aaggtattca tagagaagaa
    26401 gtaaaggata ttgatcatat tgttggaaca ttattagacg aattgtataa agaagtaact
    26461 gccaatgaag tcatagtaat ggtaaatggt atgggtggta cgccgttatc tgaattaaat
    26521 atcgtaac
//
LOCUS       DAMZPP010000019        24713 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMZPP010000019 DAMZPP010000000
VERSION     DAMZPP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24713
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.019"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..400
                     /gene="lacB"
                     /locus_tag="POH73_000610"
     CDS             <1..400
                     /gene="lacB"
                     /locus_tag="POH73_000610"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDI6886586.1"
                     /translation="THYPIFGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRS
                     ALVRDMTSALYAKEELNANVIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKI
                     KHLETSNADQADPHFFDEFLEKWDRGEYHD"
     gene            413..1345
                     /gene="lacC"
                     /locus_tag="POH73_000611"
     CDS             413..1345
                     /gene="lacC"
                     /locus_tag="POH73_000611"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDI6886587.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHAGIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEVDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPKISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            1349..2329
                     /gene="lacD"
                     /locus_tag="POH73_000612"
     CDS             1349..2329
                     /gene="lacD"
                     /locus_tag="POH73_000612"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDI6886588.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDASTHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            2351..2662
                     /locus_tag="POH73_000613"
     CDS             2351..2662
                     /locus_tag="POH73_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDI6886589.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            2668..4380
                     /locus_tag="POH73_000614"
     CDS             2668..4380
                     /locus_tag="POH73_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI6886590.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            4398..5810
                     /gene="lacG"
                     /locus_tag="POH73_000615"
     CDS             4398..5810
                     /gene="lacG"
                     /locus_tag="POH73_000615"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDI6886591.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGEYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIKDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            6076..6945
                     /locus_tag="POH73_000616"
     CDS             6076..6945
                     /locus_tag="POH73_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6886592.1"
                     /translation="MKKIFVISTTLLAVAIIIGTIITVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLSKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGESAQHSQLHENE
                     NVANELIDFLWKK"
     gene            7228..8229
                     /locus_tag="POH73_000617"
     CDS             7228..8229
                     /locus_tag="POH73_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDI6886593.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEDIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKDGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAESLAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPRAFKNLFTGDNFGKQVIKVTE"
     gene            8464..9471
                     /locus_tag="POH73_000618"
     CDS             8464..9471
                     /locus_tag="POH73_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDI6886594.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPNVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPSENIGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDY
                     IFCTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHR
                     TPDMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHLLLEKQSMIGKLVIN
                     I"
     gene            9779..11341
                     /locus_tag="POH73_000619"
     CDS             9779..11341
                     /locus_tag="POH73_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDI6886595.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITSFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYASITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLLGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIAEIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLLKSLSEEKPRNSNKVLIKHRDPDV
                     LEYRSSKHIDEN"
     gene            11516..12052
                     /gene="amaP"
                     /locus_tag="POH73_000620"
     CDS             11516..12052
                     /gene="amaP"
                     /locus_tag="POH73_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDI6886596.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRADIKKNVEHFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            12065..12304
                     /locus_tag="POH73_000621"
     CDS             12065..12304
                     /locus_tag="POH73_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDI6886597.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTILIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            12367..12876
                     /locus_tag="POH73_000622"
     CDS             12367..12876
                     /locus_tag="POH73_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDI6886598.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
     gene            complement(13037..15013)
                     /gene="sfaD"
                     /locus_tag="POH73_000623"
                     /gene_synonym="sfnaD"
     CDS             complement(13037..15013)
                     /gene="sfaD"
                     /locus_tag="POH73_000623"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HDI6886599.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDNITNMITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAMSYQALSMQHDSTPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPN
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPMIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWIETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVHSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            15115..16308
                     /gene="sfaA"
                     /locus_tag="POH73_000624"
     CDS             15115..16308
                     /gene="sfaA"
                     /locus_tag="POH73_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HDI6886600.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQSICYFIAVLLCLPLHFKVTKIPEDATRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPRVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            16295..18052
                     /gene="sfaB"
                     /locus_tag="POH73_000625"
     CDS             16295..18052
                     /gene="sfaB"
                     /locus_tag="POH73_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HDI6886601.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKESLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIMTLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAIDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYSQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEEAELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            18059..19129
                     /gene="sfaC"
                     /locus_tag="POH73_000626"
     CDS             18059..19129
                     /gene="sfaC"
                     /locus_tag="POH73_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HDI6886602.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNIQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISMADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     HINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFIQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNQY"
     gene            complement(19230..19355)
                     /locus_tag="POH73_000627"
     CDS             complement(19230..19355)
                     /locus_tag="POH73_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886603.1"
                     /translation="MYHGYIIFELIQLTPFSMTVISLIQLILPIAVLCDELLHLI"
     gene            19522..20505
                     /locus_tag="POH73_000628"
     CDS             19522..20505
                     /locus_tag="POH73_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6886604.1"
                     /translation="MRGLKTFSILGLIVALFLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIRDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNIDAFKTI
                     AKALDKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDNAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            20517..21548
                     /locus_tag="POH73_000629"
     CDS             20517..21548
                     /locus_tag="POH73_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6886605.1"
                     /translation="MAIKELSSQSAIDHKRKRRTTLTYMVSVCFLFICVYLNMAIGSS
                     KINFSDIVHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFVIVLVTIIIPSLEYYSLYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWLIGALIA
                     TVFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSIAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            21545..22513
                     /locus_tag="POH73_000630"
     CDS             21545..22513
                     /locus_tag="POH73_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDI6886606.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MIFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            22749..24119
                     /locus_tag="POH73_000631"
     CDS             22749..24119
                     /locus_tag="POH73_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDI6886607.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLLHNAFNVRIGW
                     LILRLLAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGIVDFFKSGLKTVVDMWFVILPVV
                     MSIGTIATIIANYTPLFVILGKPFVPFLELMHIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(24251..24511)
                     /locus_tag="POH73_000632"
     CDS             complement(24251..24511)
                     /locus_tag="POH73_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886608.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKISTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
BASE COUNT         8380 a         3539 c         4683 g         8111 t
ORIGIN      
        1 aacacattat ccaatttttg gtaaaaaagt tggagaacaa gttgttagcg gtaatgcaga
       61 cttaggtgtt tgcatttgtg gaacaggtgt tggtattaac aatgctgtaa ataaagtacc
      121 tggcgttcgt tcagcactag tacgtgatat gacatcagcg ctatatgcta aagaagaatt
      181 aaatgcgaac gttattggct ttggtggacg tattattggt gagttattaa tgtgcgatat
      241 tatcgatgca tttattaatg ctgaatataa accaactgaa gagaacaaaa aattaatcgc
      301 taaaattaaa catttagaaa caagcaatgc agatcaagct gatccacatt tctttgatga
      361 attcttagaa aaatgggaca gaggcgaata ccacgattaa gggacgacac atatgatttt
      421 aactttaaca ttaaatccat ccgttgatat ttcataccca ttaacagcat tgaaattaga
      481 tgatgttaat cgtgttcaag aagtaagtaa aacagctggt ggtaaaggtt taaatgtaac
      541 aagagtactt gcgcaagtag gtgagccagt ccttgcgagt ggttttattg gcggtgaatt
      601 aggtcaattt attgctaaaa aattagatca tgccggcatc aagcatgcat tttataacat
      661 taagggtgaa acacgaaact gcattgcaat tttacatgaa ggacaacaaa cagaaatttt
      721 agagcaggga ccggaggttg ataatcaaga ggctgcagga tttattaaac attttgagca
      781 attactagaa aaagttgaag cagttgctat ttcaggtagc ttgccaaaag gattaaatca
      841 agattattat gcacaaatca ttgaacgatg ccaaaacaaa ggtgtgcctg tgatattaga
      901 ctgctcgggt gcaactttac aaacagtatt ggaaaatcca tacaaaccaa cagtcattaa
      961 accaaacatt tctgaattat atcagttgct taaccagcca cttgatgaaa gtttagaaag
     1021 tttgaaacaa gcagtttcac aacccttatt cgaaggaatt gagtggatta ttgtgtcttt
     1081 aggtgcacaa ggtgcgttcg ctaagcacaa tcacacattc tatagagtga atattccgaa
     1141 aataagcgta ttaaatccgg ttggttcagg tgattctaca gttgctggaa ttacttcagc
     1201 aattcttaat catgaaaacg accacgattt actaaaaaaa gccaatactt taggcatgct
     1261 gaatgcgcaa gaagcacaaa caggttatgt caatttaaat aattatgatg atttattcaa
     1321 tcaaattgaa gttttagagg tgtaaaaaat gtcaaaatcg aatcaaaaaa tcgcatcaat
     1381 tgaacaatta agtaataacg aaggtatcat ttctgcatta gcatttgacc aacgtggtgc
     1441 tttaaaacgc atgatggcaa aacatcaaac tgaagaacca actgtagctc aaattgaaca
     1501 attaaaagta ttagttgcgg aagaattaac gcaatatgca tcttcaattt tattagatcc
     1561 tgaatacggt ttaccagctt ctgatgcacg taacaaagat tgtggattat tacttgctta
     1621 tgaaaaaact ggatatgacg taaacgctaa aggtcgttta cctgactgtt tagtagagtg
     1681 gtctgcaaaa cgtttaaaag aacaaggtgc aaatgctgtt aaattcttac tttactatga
     1741 tgttgatgat gctgaagaaa ttaacattca aaagaaagca tatattgaaa gaatcggttc
     1801 agaatgtgtt gcagaagata ttccattctt tttagaagta ttaacatacg atgacaacat
     1861 tcctgataac ggtagtgttg aatttgcaaa agttaagcct agaaaggtta acgaagcaat
     1921 gaaattgttc tcagaaccac gctttaacgt tgatgtattg aaagttgaag taccagtaaa
     1981 catgaagtat gtagaaggct ttgctgaagg tgaagtggtt tatacaaaag aggaagcggc
     2041 acaacacttt aaagatcaag atgcatcaac tcatttacca tacatttact taagtgctgg
     2101 tgtttctgcg gaattattcc aagaaacttt aaaatttgct cacgaagctg gtgctaaatt
     2161 caacggtgta ctttgtggcc gtgctacatg gtctggtgct gttcaagtat acattgaaca
     2221 aggtgaagat gctgctcgtg aatggttacg tacaactggt ttcaaaaata ttgatgactt
     2281 aaataaagta ttaaaagata ctgcgacatc ttggaaacaa agaaaataag tataaaaggg
     2341 gattaatgct atgaatagag aagaagtcca attattaggt tttgaaattg ttgcatttgc
     2401 aggggatgca cgttctaagt ttttagaagc attaacagca gctcaagctg gagattttgc
     2461 aaaagcagat gcactgattg aagaaggaaa caattgcatt gctgaagcgc atagagcaca
     2521 aacaagctta ttagctaaag aagcgcaagg tgatgatatt gcatacagtg tgacgatgat
     2581 gcatggtcaa gaccatttaa tgacaacaat tttactgaaa gatttaatga agcatttatt
     2641 agaattttat aaaagagggt gatgacaatg atgcaaaaat tgatcgcaca aattgaaaaa
     2701 gggaagccat ttttcgagaa attatcacgt aacatatatt taagagccat ccgtgatgga
     2761 tttatttctg caatgcctgt catcttattc tcaagtatct ttctattaat tgcatacgtg
     2821 ccaaatatat ttggttttaa atgggataaa ggcatggaag ccattttaat gaaaccatat
     2881 aactatacga tggggcttgt agcattttta gtagcaggaa cgacagcgaa atctttaaca
     2941 gattcattta accgtaagct agaaagtaca aatcaaatta actttatttc tacaatgcta
     3001 gcagcgatgt gtggattctt gttcttagct tctgatccag ccaaagatgg tggattctta
     3061 agtgcgttta tgggaactaa aggtttatta acagcattct tatcagcatt tgtaactgtt
     3121 attgtttata acttctgtgt gaagcgcaat attacaatta aaatgccgaa agaagtaccg
     3181 ccgaatattt cacaagtatt taaggactta attccatttt cagcggtaat catcattctt
     3241 tatgcactag atttagtcat tcgcaacagc tttaaatcaa atgtagcgga aggtatttta
     3301 aaattattcg aaccattatt tacagcagca gatggatgga taggtgtcac aattatcttt
     3361 ggtgcctttg cattattctg gtttgtagga attcatggtc catcaatagt agaaccagca
     3421 attgcagcca ttacatatgc gaatatcgaa gcgaacttca agttgcttca agctggtgaa
     3481 cacgcagata aaattattac atcaggtaca caaatgttta tcgttacatt tggtggtacg
     3541 ggtgcaacat tggtcgtgcc atttatgttt atgtggatga cgaaatctaa acgaaacaaa
     3601 gcgattggac gtgcatctgt tgtaccaaca ttctttggtg taaacgagcc gattttattc
     3661 ggggcaccac tagtattgaa tcctgtgttc tttattccat ttgtattagc gccaattgtg
     3721 aacgtatgga tatttaaact tttcgttgaa gtgttaggaa tgaacagttt tagtgtgaac
     3781 ttaccatgga caacaccagg tccactcggc atcatcatgg gtactggatt tggtttatgg
     3841 tcattcgtac tagcgattac tttaattgtt gtagatatta ttatttacta cccattccta
     3901 aaagtttatg atagtgaaat tcttgatgaa gaagaaggac gtaaagaaag taattcagat
     3961 ttaaaagaaa aagttgcagc aaactttgat acgaaaaaag ctgattcaat tttagcggca
     4021 agtggtgtat cagacgatgc agctaaagct tcaaatatca cagaacaaac aaatgtctta
     4081 gtactttgtg ccggtggtgg tacaagtgga ttattagcca acgcacttaa taaagcagct
     4141 gaagaatatc atgtgccagt gaaagcggca gctggcggat atggcgcaca tatggacatt
     4201 atgaaagagt accaattaat cattttagcg ccacaagttg cgtctaacta tgaagatatt
     4261 aagcaagata cagatagatt aggtattaaa ttagcgaaga ctcaaggtgc tgaatatatc
     4321 aaattgacac gagatggcca agcagccctt gatttcgttc aacaacaatt tgaaaattaa
     4381 gaataggagt ttttcatatg actaaaacat tacctgaaga ttttattttt ggtggagcaa
     4441 cagcagcata tcaagcagaa ggtgcgacaa atacggatgg taaaggtcgc gtagcatggg
     4501 atacgtattt agaagaaaac tattggtaca ctgcagaacc agcgagtgat ttttataaca
     4561 gatatccagt ggatttagaa ttaagtgaaa aattcggtgt taatggtatc cgtatttcaa
     4621 ttgcatggtc tcgtatcttc ccaaatggat atggcgaagt taaccctaaa ggtgttgagt
     4681 attaccataa gttatttgcg gagtgtcata aacgtcatgt tgaaccattt gtaacattgc
     4741 atcattttga tacaccagaa gtgttacaca aagatggcga ttttctaaat cgtaaaacga
     4801 ttgactactt tgtagattat gctgaatatt gttttaaaga attcccagaa gtaaaatatt
     4861 ggactacatt caatgaaatt ggaccaattg gtgatggcca atatttagta ggtaaattcc
     4921 ctccaggtat taaatatgat tttgaaaaag ttttccaatc tcatcataat atgatggttg
     4981 ctcatgcaag agcagtaaaa ttatttaaag atggcggcta tcaaggagaa attggtgttg
     5041 tacacgcatt gccaacaaaa tatccgttcg atccatcaaa tcctgaagat gttcgtgcag
     5101 cagaattaga agatatcatc cataacaaat tcatcttaga tgcaacatat ttaggtgagt
     5161 attctcgtga aacgatggaa ggtgtgcaac atatcttatc tgttaatggc ggtaaattaa
     5221 acattacaga tgaagattat gccattttag atgcagccaa agatttaaac gacttcttag
     5281 gtatcaatta ctacatgagt gattggatga gaggttatga tggagaatct gaaattacgc
     5341 ataatgcgac aggtgataaa ggcggttcta aataccaact taaaggtgtc ggtcaacgtg
     5401 aatttgatgt cgacgtacct cgcacagact gggactggat gatctatcct caaggtttat
     5461 atgatcaaat catgcgtgtt gttaaagatt atcctaacta tcataagatt tatatcactg
     5521 aaaatggttt aggatataaa gatgaattta ttgaatctga aaaaacagtt catgatgatg
     5581 cacgtattga ttatgtaaga caacatttaa acgttattgc agatgctatt aaagatggtg
     5641 caaatgttaa aggttacttt atttggtcat taatggatgt gttctcttgg tcaaatggtt
     5701 atgaaaaacg atatggttta ttctatgtcg attttgagac tcaagaacgc tatcctaaga
     5761 aaagtgctta ttggtacaaa gagttagccg aaacaaaaga aattaaataa taaaagatat
     5821 taaaaggcct atcagtcgtg tgctggtagg tctttattta taaaaagaga aaaattaaac
     5881 atgcatatac atatttttat attaatcaat gggagacaca cgattgaaag ttaaataagc
     5941 aatcaattaa caaacgttaa cggacaattt tccaaagtca tctttaaagt taatgatgta
     6001 ttcaaaatca actaaaggac atttttaatc acatccttta tacttaaata aaagacatga
     6061 atgggagtta aaagtgtgaa gaaaattttt gtaattagta cgacattgtt agcggtcgct
     6121 attattatag gcactattat tacggttgtg tttagtcaac gacaggcaca aacatttaaa
     6181 atacaacaac agcaatttgt taaaaagcca attccgacat tgtttttaca tggatttggt
     6241 ggtagtgcca actctgaaaa atttatggtg aagcaagcag aaaaaagagg ggtaactaaa
     6301 gatatcatca ctgcttacgt ttcaaaggat ggtgcagtga catttaaagg gaaactaagt
     6361 aaagatgcag tgaatcctat tgtgaaaatt gaacttgaaa ataacagaca aggctatttg
     6421 gataagaatg ctgcgtggtt taaaaatgta ctgactaagt tacaatctga atacaatttc
     6481 gataaattca actttgtcgg tcactcaatg gggaacttaa catttgcaca atatatgatg
     6541 acatatggta acgacaagtc actgccacag ttaaacaaac aagtgaatat tgctgggaca
     6601 tttaatggcg ttttaaatat gaatgaagat gtcaatgaaa ttacagttga taaagatggt
     6661 aaaccaagtc gaatgaatca accttatcag caattacgcg tgctaaaaga tatttataaa
     6721 ggtaaaggta tcgaagtgct aaatatttat ggagatttga aagatggtac acattcagac
     6781 ggcagagtgt ctaatagttc ttcgaaatca ttaaaatatt tactgggaaa tagtccgaaa
     6841 agttatcgag aatctaaata tgaaggtgag tctgcacagc atagtcagct tcatgagaat
     6901 gaaaatgttg ctaatgagtt gatcgacttc ttatggaaaa aataaagcaa ctgctagttt
     6961 ttcgggggca aaaaagcttg taatattaga gtttttcaaa aattatctat tgtttgagag
     7021 tttccataaa atgtctaagt cgtaacattt taaatttatg attatagcaa atataatgtg
     7081 gttgaagtta catgaatgaa tcagtgctta agtccaaata taacaactaa gaatgtattt
     7141 gcatacagac atcacgaaga cacaattcaa acttaacgct taagtaacaa gtaagtaaaa
     7201 gtttcaaata aagaaaggtg attaatgatg caaaataaac aaattctttt taacaaaata
     7261 ccagaaggca tgcctcaaga ggatacattc aaaattgaag acattgacac gcctaaactt
     7321 gagagcgatg gtgtacttgt acaaacgctg tatatttcag tggatccata tatgagagga
     7381 cgtatgacta aggcagattc gtatgtacag ccttttgaaa ttggtaaacc gattgttagc
     7441 catgtcgttg caaaagtaat tgattcgaca ctcgcagatt ataaaaaagg agatgtcgtt
     7501 gtaggtatgt tgccttggcg aatcattaat catgtccaag cggatcaaat tactaaagtt
     7561 ccaacgacag atgtaccact tgatttatat ttaagtgtct taggaatgcc aggacaaacg
     7621 gcatatcatg gtctgcttga tattggacaa cctaaagacg gtgatactgt tgtggtttca
     7681 gctgcatctg gcgctgtagg ttcagttgta ggtcaaattg ctaagattaa aggttgccgt
     7741 gttgtaggta ttgcgggtgg cgacaaaaaa gtaaattatt taacagagac actgggcttt
     7801 gacgctggta ttgattataa gaaagaggat tttgcagaat cgttagcaca agcagtacca
     7861 gatggtattg atgtttattt tgaaaatgtt ggtggtacag ttggcgatga agtatttaag
     7921 catttaaatc gttttgcacg tgtaccagta tgtggcgcaa tttcgtcata taatcatcct
     7981 gaagcagata tcggaccacg tattcaaggt acgcttatta agaagcaagt aatgatgaga
     8041 ggcttcttag taagtgaatt tgctaatgct tttaaagaag ctagtgaaca gctcgcaaca
     8101 tgggttcaag aaggtaaaat tcagtcacaa gtaaccattg aagatggttt tgaaaatgcg
     8161 ccacgtgcat ttaaaaattt atttacgggc gataactttg gtaaacaagt tattaaagtt
     8221 acggaataat aaagtataca tttttacaaa tcaactttgt taattgacca aaatttaata
     8281 actttcttat ctaaagcaaa tttgttcagt aacatggtag aaataagtct aaaatgtaat
     8341 acgaaagtcg tctgtaatta tttatatatt caaatgaaaa aggagtcatg atgctgcgac
     8401 ataagcacac atggcttcta ttttgtataa taagaaataa gttcataaat gaggtgaaac
     8461 gatatgaaaa tgataggatt tgaaaagccc tttaaattgg aagaaggaaa tttatttaaa
     8521 gtatacgaac aaagaaagcc aacgccagaa aacgatgata tcttagtcaa agtaaatagc
     8581 attagtgtaa atcctgtaga tacaaaacaa cgacaaatgg aagtcactca agcacctcgt
     8641 gtacttgggt ttgatgctat tggtactgtt gaagctatcg gaccaaacgt tacattattt
     8701 agtccgggtg acgttgtatt ttatgctgga tcgccaaata gacaaggatc aaatgcgaca
     8761 tatcaattgg tttcagaagc tatagttgca aaagcgccac acaacataag cgctaatgaa
     8821 gcggttagtc taccgctaac tggtattacg gcatatgaaa cgttttttga tacatttaaa
     8881 atttcacata atccttctga aaatataggt aaatccgtgt taatcattaa tggcgctggg
     8941 ggtgtaggaa gtattgccac tcaaattgct aaacggtatg gtttaactgt cattacaaca
     9001 gcatcacgac aagaaacaac agagtggtgt gaaaagatgg gcgcagatat cgtattgaat
     9061 cataaagagg atttagtgcg tcaatttaaa gaaaaagaaa ttccattagt agattatata
     9121 ttttgcacct ataatacaga cttgtattac aatactatga ttgaactgat aaaaccatta
     9181 gggcatatta caactatcgt agcttttaat gaagatcaag atttaaatgc attaaaatta
     9241 aaaagtataa catttacaca tgaatttatg tttgcgcgtc caattcatcg aacgcctgat
     9301 atgattaaac aacatgagta tttagaagat attacgaaaa atatagaatc aggacattac
     9361 caaccaacaa cgacacaagt ttttgaaggt ttatcacctg aaaacttata tcaagcacat
     9421 ctattgttgg aaaaacagtc aatgattggg aaattagtaa ttaatatata gattagtaag
     9481 taaagtcgcc ttatgcagaa aatactgtat agggcgactt attttttgtg tttagcgact
     9541 attgattcta aagtgaaata tggcaacttt gcaggtatca attcaataat gcattagaaa
     9601 ggcattactt aaatacaata aaatactttg ttgttcaatg ataaataatt tgagagtatt
     9661 ttatatatca tttttaaaag atgaagcatt attaattgtg tctatataaa attacaaatg
     9721 attaaaatgt tgcagttgtg ggtatgaact taccattaca cagaaagggt ttgtatttat
     9781 gaaaaataaa tcagtaagtc tagtgttttg ggtatcgtta gtgatttgta cgatatttgt
     9841 tgcttttggt gcaatatttc cgaaacagtt agaaaaactc acgcaaaaca ttacttcatt
     9901 tattgcgcta catttttctt ggtattattt attgctcgtt ttagttattt tatttgtctg
     9961 tgtgtatata ttattttcaa gatacgcatc aattacatta ggtgaagaag gtgaagaccc
    10021 agaattttca ttgccatctt ggtttgctat gttatttagt gctggtatgg gtataggact
    10081 tgtgttctgg acaacggcag aaccaatcag tcacgccttc aaattaacgc ctattcataa
    10141 agctggcacg caatccgcta ttaatgatgc aatgcagttt tcatttttcc attgggggat
    10201 tcatgcatgg gcagtctatg gtattgtcgc gcttgtgttt gcatatttta gctttcataa
    10261 aggatatcct ggcttagtca gcgctacatt gacgccttta ttaggtgaaa aagcaatgcg
    10321 tggcccttta ggtggtgcca ttgatgtatt ggcagtcata gctacagtaa caggcgttgc
    10381 tgcaacatta ggtttcggtg cattgcaaat aaacgaaggt ttacattttt tatttaatgt
    10441 accgagtaac ttcacgatgc aagtaatctt gattgtcatt gcaactatat tatttacttg
    10501 gtcagcatgg tcaggtatcg ataaaggtat caaaacgttg agtaatatca acatgttact
    10561 tgcttttgta gtacttcttg gtctttttat tgttgggcca acgctttaca ttttaaatac
    10621 atttacgaat ggtctaggta attatattgc aaatttcttt agtatgagtt tacgcattcc
    10681 gtctggcggt caaaaattcc aatggttaca aaattggaca atcttttact gggcatggtg
    10741 gatatcttgg gcaccatttg taggtatatt tattgcacgt gtgtcaaaag gacgtacgat
    10801 taaagaattt attctaggtg tactttttgt cccggcgtta gtttgcttta tattctttgc
    10861 ggtattcggc gcgtcagcaa tttacttaca agataatcat attgccgaaa ttgctaaagc
    10921 tgcgacagaa acagcaactt ttgcaacgct acaacattac ccactaggtt ttgtattaag
    10981 cttaattact ttacttgtta ttatgatttt ctttgttaca tcagcggact cggcaactta
    11041 tgttttaggg atgttgagtt caagtggtga tattaatcca aagtcatttg tcaaagtgag
    11101 ttggggtatc attatggcat tatttgcaat tattatgatt tacacaggtg gtacgcaagc
    11161 gatacaaaat ttattaatca tcgcagcgtt gccattttcc gtggtcatta ttgcgatgat
    11221 atggtcgctt ttaaaatctt taagtgaaga aaaacctaga aatagtaata aggttttaat
    11281 taaacatcgt gatccagacg tattagaata tcgttcttca aaacatattg acgaaaatta
    11341 attaacaaag aggttttatt tacaaaaact agggaatata ttactaaatg gtacttcatc
    11401 ttatatatta aaaataaatt taaagttatt aatttattat gtttaagaaa aaatctgatg
    11461 ggtacatatt taatatgttt ttaaattaac tttaactaag gaggtgtcgt taggggtgaa
    11521 acgacttaaa aactttatcc ttggcttatt aatagtagta atcgtggggt tcctgttatt
    11581 catgtatatc caagatggac gtataactga atatcaagac tacttccttc aatttgaatg
    11641 gttccaacca ttacttattt cacttgctgc actattgata ttaataggtc ttatattagt
    11701 atttagtatc ttcaaaccta cgcatcgaaa acctggatta tacaaagact tcgatgatgg
    11761 tcatgtttac gtatctcgta aagctgttga aaaaactgca tttgatacag ttgcaaaata
    11821 tgaccaagta agacaaccga atgtagttgc aaaactatat aacaaaaaaa ataaatctta
    11881 tatcgacatt aaaactgact ttttcgtacc gaataatgtt caggtacaat ctttaactga
    11941 agcaattcgt gcggatatta aaaagaatgt agaacatttt acagaaatgc cagtacgtaa
    12001 gttagaagta aatgtgagag atcagaaaac ttctggtcca cgagtgttgt aagggaggta
    12061 tatcatggct aacaatcata accaaaacgg acaagactct acacaacaag tgattaattt
    12121 tttaaaagta tttaaatgga gaatcgttgg tttcttagcg tttctattaa tcgcgatatt
    12181 attcttaacg ttaggttttt ggaaaacaat acttatcatc gttttatgtt taattggtgt
    12241 aggtattggg tatatgaaag accgtaagca agattttatg aattttttaa atcgatggag
    12301 ttaattcatc aatttagaat ttaattaaaa aaatataaaa aataacaagt aaaaggagaa
    12361 tctattatga ctgtagataa caataaagca aaacaagcat acgacaatca aactggtgtt
    12421 aacgaaaaag aaagagaaga gcgtcaaaaa caacaagaac aaaatcaaga gcctcaattc
    12481 aaaaacaaat taactttctc tgatgaagtt gttgaaaaaa ttgctggtat cgctgcacgt
    12541 gaagttaaag gtatcttaga catgaaaggt ggcttaactg atacattcac taatgcattc
    12601 tcaagtggca acaacgttac acaaggtgta tctgttgaag ttggtgaaaa acaagctgct
    12661 gtagacttaa aagtaatttt agaatatggt gaatcagcac ctaaaatctt ccgtaaagta
    12721 actgaattag ttaaagaaca agttaaatat attactggtt tagatgtagt tgaagttaac
    12781 atgcaagttg acgatgtaat gactcaaaaa gagtggaaac aaaaacacga aaaaaataat
    12841 gaaaacaata accaagaaag acaaggttta caataagtta ttcgataaaa aagctcggca
    12901 caccatttta gtgccgagct tttttgtgat ttttaataat ttgaatatgg aaaatgtatt
    12961 attctcttat ttgtatagat tgtatttaat aagttaatgt aatccttgag ctcacgatta
    13021 atgaaattct ataattttaa ttattttctc gatacaaagg gttgttaact ttaatataag
    13081 tataatgatg tgcttcatct tcaagccgca ttgttgtaac acacttataa tctataaatg
    13141 gcgcgaacat ggtatcttta atttcattta tacgattctc atttacttga ttagatttgt
    13201 gtgtcgaatg tacaagttga tcaaagatgt tatctagtac atcacgaacg atataccaca
    13261 tatgtctttc taagtttgaa tcattcgatg ctttagagat tgtaagaatt aattcgccta
    13321 aatggttttg aacggtagaa taaaaggctt tgttaaacac agacgttttt gaatcagtaa
    13381 gaattcttga tttttcatgg aaatgagatg tactgtatcc catttcattt agttgtgctt
    13441 tatcaattct taaaccttca aagtctctaa tatacatcgt gtccaataga ccatctttcc
    13501 gaaatgttgc aatagcattt tgcaagtgag cctctaatgc aatgccatat ttagtaacta
    13561 gtgggattac gagaccaagc aatgctttac tgtaagtttc aatccacgat ttcgccgatg
    13621 attcaaaatc agacaatgat gcagctgatt gataacgttt aatcaatgtc acgataggtg
    13681 attcattgtt aaatgggtag gttgcaacta agcttgaagg aatcattggt gtgacttctt
    13741 gtggaatcat ttggtatatg ttttttctaa ataatgtacc taattgttca cttcgttcag
    13801 tttgatagtc tgcttcgtct tgctcattat aaaaatgaat accagcaact tcatcaataa
    13861 ttgttgatgc ataggactta aatatgacat ctttctccaa aatatcattt aaaatacgtg
    13921 tcattagtgg accattgtgc gtcgtttgtt ctgataatgt acgaatttca cctgtaatat
    13981 gaacgtttgt cgacaatttg atgtgtggcg acatagctgg gtatttagga actaatgttc
    14041 tgaaagataa accggcataa taatccaacg tatgttttgc ttcaatgatt aattctttat
    14101 ctacttctgc ttgataatca gaatgtaata cgtcatctaa ttgccatgga tgtacaatca
    14161 taatgtgata atcattaaag ttaaagtttg gtgtaaattc attttccaat tgtttaatta
    14221 agtccggaaa tagttgatga acagttgtat cataatcttt agacagtgac atagtacggc
    14281 ttaatttact gtgaatcagt actattttca acttaatagg ctgattgaat tctgaagaat
    14341 ataagaatgt ttgtaatgca tttaaacctt tacgtaattt agccccggga tgtagcggat
    14401 gaccttcaat aacggcttgc tctgaacgca agtaactatc ttcgctattt tcgataatat
    14461 taaataaagg tgtagaatca tgttgcattg acagtgcttg atagctcatc gcaaatgtca
    14521 tattagttgc actgtttatt aaatcttgct gaaattgatc actagcagca ttttttaaat
    14581 ctggtgcttc aattaaaata cactcaagaa tttcatttgg atggtgtact cgtgtaatca
    14641 tatttgtaat gttatcttta atgtagaaag ggccttcaac atcaattcga tcaaaggcgt
    14701 gttctccagt gataggagca tataatgttt gcttagcttg tgggaactgg atttctagta
    14761 tatgagtcgt tgagatatct aacataatca aatcacgact caatattttc ttactttgag
    14821 tgcgcgcttt aactaagttt tcgcgatgca ttgatgtgac taatctctga gtgactttat
    14881 ctcttccttg taaaatcatc tctttaaaaa tattagccca atcgctatta tgttgttgta
    14941 aaaataaata tgtttcttgt tcttctttgt taaattttaa tgtctgttct ttaaaaatta
    15001 agttcaagtt cataattcac ctctatgaaa tattttacaa aagcaagata gatttgtata
    15061 atccatatta atgataatga ttcttattat caacagaatg cgggtgtaag ttttatgaca
    15121 aaatattttt ttagcagttc ttttctacta tttctaggta attggattgg acaaataggg
    15181 ctaaattggt ttgtacttac cacttatcat aacgcagttt atctggggat tgtcaatttt
    15241 tgcagacttg taccaatatt attactaagt gtgtgggcag gggcaattgc cgataaatat
    15301 gataaagggc gattgctgag aattacaatt tcatcatcat ttttagtaac tgcaatttta
    15361 tgtgtgctta cgtatagttt cactgcaatt ccaattagcg tcattattat atatgcgaca
    15421 ttaagaggga ttttaagtgc agttgaaaca cctttaagac aagcaatctt accagattta
    15481 tcagataaaa tatctactac acaagctgta tcatttcatt cattcatcat taatatttgt
    15541 cgttcaatag ggcctgccat tgctggtgtc atattagcag tctatcatgc gccaacaaca
    15601 tttcttgcac agtcaatttg ttattttatc gcagttttat tatgcttacc attacatttt
    15661 aaagtaacta aaatacctga agatgcaaca aggtacatgc cgttaaaagt cattatagat
    15721 tacttcaaat tacatatgga aggtcgacaa atatttataa catcattatt gattatggcg
    15781 acaggttttt catatacgac acttttacca gttttaacaa acaaagtatt tccggggaaa
    15841 tctgaaatat ttggtatcgc tatgacgatg tgtgccattg gtggtattat tgcaacgcta
    15901 gttttaccta gagtacttaa atatattggt atggtaaata tgtattattt aagttcattt
    15961 ttatttggta ttgctttgtt aggtgtggta tttcacaata ttgtcatcat gttcatttgt
    16021 attacattga ttgggttatt tagtcaatgg gcacgtacga caaatcgcgt ttattttcaa
    16081 aataatgtta aagattatga acgtggtaaa gtactgagta ttattatgat ggatagaggt
    16141 atgattccat tgggaagtct gttgatgagt atatgtgcag atgtgtttgg cattgttaga
    16201 actttttcaa taatgggaat aagtactata tgcattacaa tggtattcta ttttataaat
    16261 agaaagttga agttaaagtt ggaggaaagt aatcatggta tatcttgaat gggcaaaggc
    16321 agatagaaat attcaatatc gtgtaattaa cgccattatt aaagaacgta tttaccccga
    16381 gcaaacattt atttcacaaa aagaatcttt aattgaaatt cagtatcata tgcatgtgtt
    16441 gactattgaa gttgttagaa aaagtgcatt agaacgctat gagtttacag gtgatattac
    16501 ttatttaaat aaaggtgaaa cgtcgctaat catgacgtta gaagggctac tagatgtgtt
    16561 gaatcatgac tttgatatcc ctatttcaga gcgactacgc gaagagttaa tacacagtcg
    16621 agatagttta gttgaaacat ataagcaaat gtctcacaga caaacattaa taagtcaaag
    16681 ttttaaattt tcaagattac cacaagatat taactttttt tcatggttac aacatgtaaa
    16741 agatagtgat aagacagatg atttaactta ttctgagagt ttggtaccag aggggcaccc
    16801 aacacaccct ttaaccaaaa cgaaattgcc cttaactatg gaagaagtac gagcatatgc
    16861 acctgagttt gaaaaagaaa tccctttgca aattatgatg attgaaaaag accatgttgt
    16921 gtgcacagct atagatggta atgatcaatt tattattgat gaaataattc ccgaatacta
    16981 caatcagatt cgtgtgtttt tgaagagttt aggtttgaaa agtgaagact atagagcgat
    17041 tttagtacat ccttggcaat atgatcatac gatagggaaa tattttgaag catggattgc
    17101 taaaaaaata ttaattccaa cgccgtttac aatactttca aaagcaactt tatcattcag
    17161 gacgatgtct ttaattgata aaccatatca tgttaagttg cccgtcgatg cacaagcaac
    17221 aagtgccgtt agaacagtct caactgtgac tactgtagat ggaccaaagt taagttatgc
    17281 tttacaaaac atgttgaatc aatatccagg atttaaagtt gctatggaac cgttcggtga
    17341 atatgcaaat gttgataaag atagggcgcg tcagcttgct tgtattataa gacaaaagcc
    17401 tgaaattgat ggaaaaggtg caacagtagt tagtgcaagt ctagttaata aaaatccaat
    17461 agatcaaaaa gttatcgtgg atagttactt agagtggtta aatcaaggaa ttactaaaga
    17521 aagtattacg acatttattg aacgatactc tcaagcatta atcccgcctt taattgcttt
    17581 tattcaaaat tatggaattg cattagaagc acacatgcaa aatacagtag tgaacttggg
    17641 gccacatttt gacattcaat ttttagtgag agatttaggt ggttctagaa ttgatttaga
    17701 aacattacaa catcgtgtat cagatattaa aattacaaat gatagtttaa tagctgattc
    17761 tatagatgca gtgattgcaa aattccaaca tgctgttatt caaaatcaaa tggcagaatt
    17821 aatccatcat tttaatcagt atgattgtgt tgaagaagcc gaattattta acatagtaca
    17881 gcaagtagta gcgcatgcca ttaacccaac attaccacat gcaaatgagt taaaagatat
    17941 tttgtttgga ccgacaatta ctgtcaaagc gttgttaaat atgagaatgg aaaataaagt
    18001 aaagcaatat ttaaatattg agttagataa tccgataaaa aaagaggtgt agtactacat
    18061 ggcacacgtt aatataaata tatcgaagat taaatataac gccaaagtac ttcaaacagt
    18121 ttttcaaagt aaaaatattc aattcacacc agtaattaag tgcatagctg gtgaccgtac
    18181 aattgtagaa agcttaaaag cgttaggtat caatcatgtt gcagaatcca gattggataa
    18241 cataattagt atggcagatc aggatttaac atatacgtta ttgcgtacgc ctgccaaaaa
    18301 agagatttca gatatgatag aaaaagttga tatgagtatc caaacagaat tatctaccat
    18361 acatcatata aatgaagtag cagaagtttt aggaaaaaaa cataagattt tattaatggt
    18421 agattggaaa gatggccgtg aaggtgttct gacttatgat gttttagatt atattaaaga
    18481 gattattcac ttgaaaaata tccattttgt tggtttagca ttcaatttta tgtgttttaa
    18541 atctgatgcg ccaagtgatg atgatatctt tatgattaat cgattcgtct cagctgttga
    18601 aagagaaata ggatatcgat tgaaaattat ttcaggtggt aattcgagca tgttaccaca
    18661 acttttatat aatgatttgg gtaaaataaa tgaattgcga ataggtgaaa cgttgtttag
    18721 aggtgttgat acaactacaa atcaagcgat agctatgtta tatcaagatg cgattacatt
    18781 ggaggctgag attttagaaa ttaaacctcg agttaataca caaacgcatg aatcattcat
    18841 acaagctatt gtagatattg ggtatttaga tacaaaagtc gataatattt cacctatgga
    18901 tcagcatatc aatattttag gcgcttcgag tgatcattta atgcttgatt taaatggtca
    18961 aggacactat caagtaggag atcatattag tttttcactt aattatgaag cactttctca
    19021 ttcgatgtat atgaaaaatt tacataaggt atatattgat gattcaaaga ttgatacgct
    19081 acttcaaaat tttgatgtga aatctcctgc tatggtgaac caatattaat atatttatca
    19141 ataagtctaa gttgacaagt tatactacat atcgtttgca cggttgtatg ataattgttc
    19201 aacttagatt ttttgtattt aatgatttat caaattaagt gcaacagttc gtcacataaa
    19261 actgcaatag gtaatatcag ctgaattaaa gagataacgg tcatgctaaa tggtgtcaat
    19321 tgtattaatt caaagatgat atagccatga tacattatca gtattttgtc taaggaaatg
    19381 tgctaattgt agtcataatt attagaggaa ataaatatag tcatacattt tagtaatata
    19441 aaaagattga acgctacttg acaatgataa ttgttatcaa taaaataata aatgaagtta
    19501 tacatattaa ggagtggaac gatgagaggt ctaaaaactt ttagtatatt gggattaata
    19561 gttgccttat ttttagttgc agcttgtggt aatacggata attcaagtaa aaaagaatca
    19621 tcaactaaag atactatttc ggtaaaagat gaaaatggta cagtaaaagt acctaaagat
    19681 gcaaaacgta tcgttgtatt agagtactca tttgcagatg cattagcagc attagacgtt
    19741 aaaccagttg gtattgctga tgatggtaag aaaaaacgta tcattaaacc agttagagaa
    19801 aaaattcggg attatacttc tgtaggtaca cgtaaacagc caaacttaga ggaaattagt
    19861 aaattaaaac cggatttaat tatcgctgat agcagtagac ataaaggtat taataaagaa
    19921 ttaaacaaaa ttgcaccaac attatcatta aagagttttg atggtgacta caagcaaaat
    19981 atcgatgctt tcaagacaat tgcgaaagct ttagataaag aaaaagaagg cgaaaaacgt
    20041 cttgctgaac atgataaatt aatcaataag tataaagatg aaattaaatt tgatagaaat
    20101 caaaaagtgc ttccagcagt tgttgcgaaa gctggtttat tagcacatcc aaactattcg
    20161 tatgttggac aatttttaaa cgaacttgga tttaaaaatg cattaagtga tgatgtaaca
    20221 aaaggtttaa gtaaatactt gaaaggacct tacttacaat tagatactga acatttagct
    20281 gacttaaatc ctgaacgcat gattattatg acagataatg ctaaaaaaga ttctgctgaa
    20341 ttcaagaagt tacaagaaga tgcaacatgg aaaaaattga atgcagttaa aaataatcgt
    20401 gttgatattg ttgaccgtga tgtttgggca agatctcgtg gcttaatttc ttctgaagaa
    20461 atggctaaag aacttgttga attatcaaaa aaagaacaaa agtaaggtgg aagtaaatgg
    20521 ctataaaaga attaagtagc caatctgcca tagatcataa aagaaaaaga cgcacaacac
    20581 tcacgtatat ggtgagtgtg tgctttcttt ttatttgtgt atatttaaat atggcaattg
    20641 gttcttcaaa aattaatttt agcgatatcg ttcactatgt tactggtcat acggatacaa
    20701 aagcaacgtt tttattacat aatgtacgta tgccaaggat gattgcaggg ttatttattg
    20761 gcggtgcgtt agcggtatct ggtttgttaa tgcaagcaat gactcgtaac ccactggcat
    20821 ctccaaagat ttttggtgtt agttctggcg cgtcatttgt cattgtatta gtaacgatta
    20881 ttattccatc attagaatac tactcacttt atttaggtgt cattggcgcc tttattggtg
    20941 gattaactgt ctacacactt tctggtgcta caaaaggtat gacgcctatt aaattagcgt
    21001 tggctggtat ggccattcat ttatttttta gtagtatgac tgaagggatt ataatcttaa
    21061 atgaaaattc taatgaacaa gtgatgtttt ggcttgttgg ttcattatcc agtatgaagt
    21121 gggatgagat tttaaccatt ctaccatggc ttattggtgc attaattgca acagtcttta
    21181 ttggtcgtca gttaacgata atggaacttg gcgatgatat tgctaaggga ttgggtcaaa
    21241 atataaataa agttagaatc atcattggac tattagttat tattcttaca ggtatgtctg
    21301 tatcaatagc aggaccaatt ggctttgttg gtttaattgt gccacatatc gtcaagcgct
    21361 atgtgagtaa gaattattta gtcatgattc cattaacatt tattataggt gcagatttat
    21421 tactactatc agatgtatta agtcgtttaa ttacatatcc ttatgaatca ccagtaggga
    21481 ttgttacatc attcgtaggt gcactttact tcttatttat aacgattaaa ggggtgaaaa
    21541 gaatatgatg acaacgaaaa ttatacgtcg ttatagttta atcacaatcc ttttaattgt
    21601 gagtatattc ataagtttat gtgtagggtc tgtcatgatt cacccgattg atgctattaa
    21661 gggaatcttt acacaagatg attttatttt aaatgagtat cgtataccta gaacgttact
    21721 tggtattatt atcggtagta gcttggctat atcaggtgca attatacaag ctgtaataag
    21781 aaacccgtta gcctcacctg atgttatagg tatttctaaa ggtgccagtt tagctgctgt
    21841 gattattatt atgatctttc caacagcacc attgtttgta ttacctatag gttcattctt
    21901 aggtgccttt gcagtaagtt tatttttatc atttttaatt tcaaaatatg atgttaaagg
    21961 ttcaaagctt gccttaattg gtttagcaat aggcgcaata tgtacggcga ttgtgcagtt
    22021 cttattaatc agaaatccac tggaagcaaa caaagcgctt gtttggttga cgggtagtct
    22081 atatggacat gatatgtcga atatttatac tatattacct tggtttttaa taacgatacc
    22141 aatcatcatt ttattgagtt atcaattaga tgtattgaat ctaggtgatg ctgttgcaac
    22201 cgcactcggt ttaaaagtta aaactattaa aatgctattg ctaattttag cagtaatatt
    22261 agcaggatcc gcaatttcag ttgtcggtgg tattagcttt ttaggattaa ttgcacctca
    22321 tattgcgaga cagttaatag gaaataaaac attacacgtt attatcatgt cggggttaat
    22381 tggtgctata ttgttaacat ttggtgatgg tttagcccgt ggcattcatc cgccactgga
    22441 tattcctgta ggggttatca ttgcaattat cggtgcacca tacttcttaa tattattacg
    22501 aagaatgaaa taactaacat atgattagag tttataaaag gttaagtacg aaatcagtaa
    22561 ttgtacttaa ccttttttga attcatcttg aaaaattaca agaaatagcg tatatttaat
    22621 aactgtaatt attcattatt ttgcatacat aaacatttta aaagatataa tgataaggaa
    22681 tatgtttgtt aactgaatag aattctgtat atacttaaaa cgaatacata gagaaatgga
    22741 ttgattttat ggatagcttt tcaaggaaag agatagtcat tgggagactt aagtttatta
    22801 cgatgtcttt aattggtatc ttattatttc tagtaccaat tccagtcgaa caagatggac
    22861 agaagcaaac aacgttgcca gtggcatttt tagcaggcgt tcttaaagat gtattaggtg
    22921 gcgtaatgcc atttttaatt gtgacgataa ttactttgtc gggtatcata acactgattt
    22981 gttcaactat tttaaaagat aaattgaaac ctgatggatt attgcataat gcgtttaatg
    23041 taagaatagg ttggttaatt ttgcgattac ttgccgttgt ctttgcgtgg atgacatttt
    23101 tacgaattgg ttcaaaagta atatattcag atgaaacggg tggattattg ttctcgagtt
    23161 tattgccaac tttagtggca gtattcttat ttgcggcatt atttttgcca ctattaatgg
    23221 aatacggttt gttagagatg ttgggtccta tttttagacc ggttatgaga cctttattta
    23281 cattgccagg acgttcgacg gtagataatt tagcatcatt tattggagac ggaactgttg
    23341 gggtcttaat tacaagtaga caatatggtg aaggatatta ttctcgtcgt gaggcaacag
    23401 ttatttcaac gacatttagt gtagtttcta tcacctttgc aattgtagtt gctgaaacag
    23461 tacatatgca aaatcaattt tttgctttct atttatcagt gatagtatcc tgcctcgttg
    23521 cagcagtcat tatgccaaga atttggcctt taaataaaat tcctgatgag tatgctaagg
    23581 aggtacctga aagtgcgcgt actgaggcat tgccagaagg aaagacggca ttgagacatg
    23641 gctttgatac tgcaacagaa gtaggtatta aagcaccggg cattgttgac ttctttaagt
    23701 caggattgaa aacggttgtt gatatgtggt ttgttatttt acctgttgtg atgagtattg
    23761 gtactattgc taccattatt gcgaactaca cgccattgtt cgttattttg ggtaaacctt
    23821 ttgttccatt tttagagtta atgcatatac ctgaagcggc acaggcttct caaacgatta
    23881 ttatcggttt tgccgatatg tttttacctt ctatattaat tgaaggtgta caaaatgata
    23941 taactcgttt tgttattggg gcacttagta tatcgcagtt aatatacttg tctgaagtag
    24001 gtggcgtcat acttggttcg aagattcctg taagcatcgg caaattgttt atgattttct
    24061 tgattagaac aatcattaca ctaccaatta tttctttgat ggcacacttg ttattgtaat
    24121 tagatataga aaaggacaac tgttaaaatt gtgaaaacag ttgtcctttt gtatttgttt
    24181 atgctgggtg ttgaatcaat taaaaaaatc attcatgaag ccctcaccat gaatgatttt
    24241 tttatacaat ttatttagtt aattcattgt aaactttttg atcattttga gtataaataa
    24301 tgtattcgtg gtcttcagta tcgataataa cgcgattcgt tttaccaaat gttgagccga
    24361 ttctagatac atctttgctg tctaatttag gaactgcgtg aatatcttgg tcttgtgtaa
    24421 tatttttaat ttcacttgta gaaattttga tatcagcaac tctccattga atacgcactt
    24481 cattattatc cttttttact gtcattgcca ttattcaaac acatccttta aatattttgt
    24541 aaatacatca taaacgattt tgtaatcgtt ttcaagagtt atgtgatact tttttaaatt
    24601 gtgtcatact aatgaagttc aacccgaaaa tatgttcgtg tttttggcga atttatggta
    24661 aaatatgagt attaaaggag aacaaatatg acaggtaaaa cacatgcttc atg
//
LOCUS       DAMZPP010000020        24578 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMZPP010000020 DAMZPP010000000
VERSION     DAMZPP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24578
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.020"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..140
                     /locus_tag="POH73_000633"
     CDS             <1..140
                     /locus_tag="POH73_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDI6886609.1"
                     /translation="VNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYF
                     K"
     gene            182..766
                     /gene="dhaL"
                     /locus_tag="POH73_000634"
     CDS             182..766
                     /gene="dhaL"
                     /locus_tag="POH73_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDI6886610.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVAKDDMDNQDFIKL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            759..1121
                     /locus_tag="POH73_000635"
     CDS             759..1121
                     /locus_tag="POH73_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDI6886611.1"
                     /translation="MPKIILVSHSKEIANGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEALTKLDDDALCFYDIGSSEMNLDMAIEMYDGNHHVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            1237..1734
                     /locus_tag="POH73_000636"
     CDS             1237..1734
                     /locus_tag="POH73_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886612.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            2190..3257
                     /locus_tag="POH73_000637"
     CDS             2190..3257
                     /locus_tag="POH73_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886613.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYVTLLLDFNKIVRALGVVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKKYHIFIVIMMIAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            3408..4451
                     /locus_tag="POH73_000638"
     CDS             3408..4451
                     /locus_tag="POH73_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6886614.1"
                     /translation="MNKDNKWTMITALFIAVISVLLAFHLKQHYDQTTNENHANKDKI
                     NIKNKNVRIYQNLTYNRVYPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVVIGG
                     DSAGAQLASQFTAIQTNNQLRKAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELKVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            4778..5206
                     /locus_tag="POH73_000639"
     CDS             4778..5206
                     /locus_tag="POH73_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886615.1"
                     /translation="MTRSALKPFKNKRVMVTGRIHRVLFKNYLDRHSTFKPNVRILLK
                     DVIVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGNLVTEEAYIQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(5404..5910)
                     /locus_tag="POH73_000640"
     CDS             complement(5404..5910)
                     /locus_tag="POH73_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6886616.1"
                     /translation="MAHIIRRVSIKDVENFISMLAKIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            6023..6946
                     /gene="graX"
                     /locus_tag="POH73_000641"
                     /gene_synonym="apsX"
     CDS             6023..6946
                     /gene="graX"
                     /locus_tag="POH73_000641"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HDI6886617.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTTVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIVETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESSVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            6962..7636
                     /gene="graR"
                     /locus_tag="POH73_000642"
                     /gene_synonym="apsR"
     CDS             6962..7636
                     /gene="graR"
                     /locus_tag="POH73_000642"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HDI6886618.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVIDLSKDSIQKGDQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEIGMDSAIETKVGK
                     GYMAHE"
     gene            7629..8669
                     /gene="graS"
                     /locus_tag="POH73_000643"
                     /gene_synonym="apsS"
     CDS             7629..8669
                     /gene="graS"
                     /locus_tag="POH73_000643"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HDI6886619.1"
                     /translation="MNNLKWVVYFLKSRKNWIFWILFLNILMLGISLIDYDFPIDSLF
                     YIVSLNLSLTLIFLILTFFKEVKLYRHFEKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ILAHKDKVVDQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEENQERKQALLFEWSR
                     INSMLDTQLYITRLESQRKDMFFDYVSLKRMVIDEIQLTRHISQVKGIGFDIDFKVDN
                     HVYTDIKWCRMIIRQILSNALKYSENYNVDISTELIDQHVALIIKDHGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVDSVKDQLGIQLQVTSTIGKGTTVKLIFPLQNEI
                     VERMSEVTNLSF"
     gene            8816..9577
                     /gene="vraF"
                     /locus_tag="POH73_000644"
     CDS             8816..9577
                     /gene="vraF"
                     /locus_tag="POH73_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HDI6886620.1"
                     /translation="MTILEVKQLTKIYGNKKMAQEVLRDINMSVEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGHKLEKLSNKALSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKDTMLDRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLMKMNEEIKTTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGINYEF"
     gene            9567..11456
                     /locus_tag="POH73_000645"
     CDS             9567..11456
                     /locus_tag="POH73_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6886621.1"
                     /translation="MSFNEIIFKNFRQNLSHYAIYLFSLITSVVLFFSFVALKYTHSI
                     NITETYPIIKEGSQVGSYFLFFIIVAFLLYANVLFLKRRSSELALYQTLGLSKFNIIY
                     ILMFEQLLIFSITAILGITIGIFGSKILLMIVFTLLGIKEKVPIIFSFRAVFETLLLI
                     GVAYFLTAAQNLILVLKQSISELSKHNQAKQMNHNKITFEEVILGILGIILIISGYYY
                     SLRIVHYYDAIGVLMFILFATVIGAYLFFKSSVSLVFKIIKSFKKGVISVNDVMFSSS
                     IMYRIQKNAFSLTVMAIISAITLSVLCFAAISRATLLSEIKYTSPHDVTIKDQQKANQ
                     LASELNNRKIPHFYNYKEVIHTKLYKDDLFDIKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGAIKDLVKHKKHGKAVIGTKKHHVDIKLRKDINKIYFMTDVDLGGPTFVLND
                     QDYQEIRKYTKPKHIVSQFGFDLKHKKEMLALEKATNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGISFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMAKGLKFKIA
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPIFIVMGLYICMYAIFAMTAYNHSKRT
                     IRHSI"
     gene            12072..12689
                     /locus_tag="POH73_000646"
     CDS             12072..12689
                     /locus_tag="POH73_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HDI6886622.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
     gene            12705..13712
                     /locus_tag="POH73_000647"
     CDS             12705..13712
                     /locus_tag="POH73_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDI6886623.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(14299..15096)
                     /locus_tag="POH73_000648"
     CDS             complement(14299..15096)
                     /locus_tag="POH73_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDI6886624.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ANRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
     gene            15457..16101
                     /locus_tag="POH73_000649"
     CDS             15457..16101
                     /locus_tag="POH73_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDI6886625.1"
                     /translation="MSQNTNHSYYHQNQHAQAISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKDILYLANMFRR"
     gene            16783..18935
                     /locus_tag="POH73_000650"
                     /pseudo
     CDS             16783..18935
                     /locus_tag="POH73_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            19081..19440
                     /gene="sarX"
                     /locus_tag="POH73_000651"
     CDS             19081..19440
                     /gene="sarX"
                     /locus_tag="POH73_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HDI6886626.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEKERLSKVRSSKDERKIYIYLNDDD
                     ISKFNALFEDVEQFLNI"
     gene            19630..20346
                     /locus_tag="POH73_000652"
     CDS             19630..20346
                     /locus_tag="POH73_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6886627.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            20346..20819
                     /locus_tag="POH73_000653"
     CDS             20346..20819
                     /locus_tag="POH73_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDI6886628.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYAVPK
                     GTIFASSIEISNTYSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            20911..21027
                     /locus_tag="POH73_000654"
     CDS             20911..21027
                     /locus_tag="POH73_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886629.1"
                     /translation="MLQLILMKIALNDFEKDKSVLFIFLKNHFYEEMCVLSN"
     gene            21172..21816
                     /locus_tag="POH73_000655"
     CDS             21172..21816
                     /locus_tag="POH73_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDI6886630.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKIHQKPQNKTNFEKSKQIKPKQYNQTKNHQQQKKN"
     gene            21934..22800
                     /locus_tag="POH73_000656"
     CDS             21934..22800
                     /locus_tag="POH73_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6886631.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKQWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMTSFVEEPKA"
     gene            22932..24152
                     /locus_tag="POH73_000657"
     CDS             22932..24152
                     /locus_tag="POH73_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDI6886632.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            24149..>24578
                     /locus_tag="POH73_000658"
     CDS             24149..>24578
                     /locus_tag="POH73_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HDI6886633.1"
                     /translation="MTIVLWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQ"
BASE COUNT         8830 a         3162 c         4268 g         8318 t
ORIGIN      
        1 cggttaatgt tgctaaatgg tttgttggtg attatatgac atctttagac atgcaaggtt
       61 tttctataac tatcgtgcct aataaaccag aatatctgga agcattttta gcaccaacaa
      121 caagtcaata ctttaaataa gcagtgaata tgaataaata tacatttatg aggtggcaca
      181 aatgaaagtg aatgatatga aagcgcgttt attaaattta gaagaaactt ttaaaaaaca
      241 tgaatctgaa ttaactgaat tagatcgagc aattggtgat ggtgaccatg gggttaacat
      301 ggttcgtggg tttagtagtc ttaaagacaa acttgatgat agctcaatgc agtcattgtt
      361 caaatctact ggtatggcat tgatgtcaaa tgttggcggt gcatcaggac cactgtatgg
      421 ctttagcttt gttaaaatgt ctgcagtcgc caaagatgat atggataatc aagattttat
      481 taaactaatt caggcatttg ccgaagcggt tgaatcacgt gggaaagtta ctttaaatga
      541 aaagacaatg tatgatgtag tagcacgagc agcagagaag cttaaaaatg gtgaaacttt
      601 aacatttaat gatttacagc aattagcaga taatacaaaa gatatggtag caacgaaagg
      661 tagagctgca tattttggag aagaatcaaa aggttatata gatccaggtg ctcaaagcat
      721 ggtttatatt ttaaacgctt tgattggaga tgaagataat gcctaaaatt atacttgtta
      781 gccacagtaa agaaattgca aatggtacaa aatctttgtt aaagcaaatg gctggtgacg
      841 ttgatattat accaatcggg ggattaccag atggttcaat tggaacttca tttgatatca
      901 tccaagaagc tttgactaaa ttagatgatg atgcattgtg tttttacgat attggatctt
      961 cagaaatgaa tttagatatg gcaattgaaa tgtatgatgg taatcatcat gtgttaaaag
     1021 ttgatgcgcc aattgttgaa ggtagtttta tcgcagcagt aaagctgtca attggcggtt
     1081 caattgatga tgcattagca gaaatcaaac aatcatttta gttaaaattt actaataatg
     1141 aaaaatgtaa acctttttca aatgaaactt tataaaaaat atgatagtat atatgtaaat
     1201 gtttaataaa atctggagaa ataggaggac attgccatgc aacaccttat aaaaaaacat
     1261 gtattgaatg gcgagtttga tttagtacga caattgatgt ccgaaacaga ttttatggaa
     1321 tttgaagaag catatatttc aagtgcgcat gaagtagaaa gtatgatgtt ttatacatgt
     1381 attttagata tgattaagta cgaagaatca tctgaaatgc atgacttagc atttttattg
     1441 cttgtgtatc cactaagtga atatgaaggt gctttggatt ctgcttatta tcatgcagac
     1501 gcttccataa aactaactga cggcaaagaa gttaaaagtt tgttacaaat gttattattg
     1561 catgcgatac caacacctgt tatttcagat aagaaggctt ttgatatcgc caagcaaatt
     1621 ttaaaattag atcctaataa taatgttgct cgtaacgtct taaaagacac cgccaaacgt
     1681 atggacaacg ttgttgttga tataaatgaa ttacaccaac gtaatgcacg ttaattacat
     1741 ttcaattata ttagcttaat aatagtttta acatttggtt gggttgggac atatgttcca
     1801 gcctttttta atacttaaaa actaacgaag taaacttgtg tgcacaaatg gtttttatac
     1861 aacattttat aaatttatac attttaataa agaacatacg atagatagtt taaacctttt
     1921 taactgagaa attttgatat gtattttcga aacttaacta aaaatacgta agtcaagaag
     1981 cacaattatt aatcattttt cctataaaaa agttcgtatg actgcattat aaaagcataa
     2041 atttataatt tttttaaatg tcattgaacg tgataatgtg aatggattga gcaattttga
     2101 aaaagtgaaa aataacctat gcgacttgca attaattttc agtacgttat aatgcacact
     2161 gtgcaaaatt aaggaggtct attattcaca tgatgatgaa taaagaagca acaaaaattg
     2221 gatttgccta cgtcggcatt gtagtgggcg caggattttc aacaggacaa gaagttatgc
     2281 aatttttcac taaatatggc ttgtgggctt atttaggtgt cattatatct ggttttattt
     2341 tagcttttat tgggcgccaa gtagcaaaaa ttggtactgc ctttgaagcg acaaaccatg
     2401 aatcaacatt acaatacgta ttcggtgaaa agtttagtaa agtctttgat tatattttaa
     2461 tcttcttctt atttggtata gctgtaacca tgatagcagg tgcaggcgca acatttgaag
     2521 aaagttataa catacctaca tggttaggtg cattaattat gacattagca atttatgtaa
     2581 cattgctatt agactttaat aaaatagtac gtgcccttgg tgtcgttaca ccatttttaa
     2641 ttgttttagt tgtattaatc gctggcgtgt atttatttaa aggtcatgtt tcattagcag
     2701 aagttaacca agtagtgcct gaagcaagta tctggaaggg aatctggttt ggtacaatat
     2761 atggtggatt agctttttct gtaggtttta gtaccatcgt agcaatcggt ggggatactg
     2821 aaaagcgtac agtgtcaggt gcaggcgcga tgtacggtgg tattatctat actgtattac
     2881 tagcattgat caactttgca ttgcaaagtg aatatccaac tattaaaaat gcctcaattc
     2941 caacattaac attagccaat aacattcatc cgttaatagc aacagtgtta tctgttatta
     3001 tgctggcggt tatgtacaat actattctag gactaatgta ttcatttgca gcacgtttta
     3061 cagaaccgta tagcaaaaaa taccatatct ttattgttat aatgatgata gcaggttatt
     3121 tattaagttt cgtaggattt gctgaattaa ttaataagtt atatacaatt atgggatatg
     3181 taggcctatt tattgtagta gctgtaatta ttaaatattt caaacgtaaa aatgcggata
     3241 aaaaacatat tgcttaatat catatgatgg atatccgaaa ctttacaatt gaatcacttt
     3301 gttttaatct taaaagcaat tcgtctctac tcttatcggg cgaattgctt tttatattta
     3361 ttcagtctat taatatgagc gtctaacaaa tagagaggta cgatgtaatg aataaagata
     3421 ataaatggac gatgataact gcgcttttta tagcagtaat cagtgtattg ttagcatttc
     3481 atctgaaaca acattatgac caaactacaa atgagaacca tgctaataaa gacaaaatta
     3541 atattaaaaa taaaaatgtg cgcatttatc aaaaccttac atacaataga gtttatccta
     3601 acagtaaatt agatattatt acacctgttg atatgtcttc taatgccaaa ctgccagtta
     3661 ttttttggat gcacggtggt ggttatattg cgggtgataa gcagtataaa aacccattat
     3721 tagcgaaaat tgctgaacaa gggtacattg ttgtgaatgt aaattatgca ttggcgccac
     3781 aatataaata tcccacacca ttaattcaaa tgaatcaagc gactcaattc attaaagaaa
     3841 ataaaatgaa tttacctatt gattttaatc aagtagttat tggcggtgat tctgctggcg
     3901 ctcaattagc aagtcaattt actgcaatac agacaaataa tcaattaaga aaagccatga
     3961 aatttgatca gtcattcaaa ccatcgcaaa ttaaaggtgc tatactattt ggtggttttt
     4021 ataatatgca aacagttaga gaaactgagt ttccgcgaat acaattattt atgaaaagtt
     4081 atacaggtga agaagattgg gaaaagagtt ttaaaaatat ttcacaaatg tcgacagtaa
     4141 aacaatcgac aaaaaattat ccaccaacat ttttatctgt tggagatagt gatccattcg
     4201 aaagtcaaaa tatagaattc agtaagaaat tacaagaatt gaaagtacca gtagatacat
     4261 tgttttatga tggtacgcat catttacatc atcagtatca atttcacctt aataaacctg
     4321 aatcgataga taatatcaaa aaagtgttac ttttcttaag tcgtaataca tcttctagtg
     4381 gtattcaaac tgaagagaaa ccacaaatag aaaatccgag taatgaatta ccgttaaatc
     4441 cattaaacta atgataaaca gtagtaattt attacttaag caacatttaa gatcttcaaa
     4501 ttgaaaacga agaatttaaa acatgtgggg ctaatgtgta agtgtctgag atataataaa
     4561 ttgttagcgg ttctttatca tttctgtccc ccctattatg atgtgacatt tatttacaaa
     4621 ctaatttgtt ttgaacctga aaataacttt ttacaactaa atagtcgaaa aacagtgtgt
     4681 acatgatata ataaatttat aaattgaaaa gaattcaaaa gaaaaattaa aatatagatt
     4741 gagcacgaag tgatttgaaa taaggttgtg aaagggaatg acaaggtcag cattaaaacc
     4801 atttaaaaat aaacgcgtta tggttactgg acgtatacat cgtgtgttgt ttaaaaatta
     4861 tttagataga catagcacat ttaagccgaa tgtaaggata ttattaaaag atgtaattgt
     4921 ttcaggtgtt tcaatagatc atttatggct atatgagaca aataaatact atgcattagc
     4981 aatggaactt attcatcaac gagtaaaatt tagtgcgaat gttgtaccgt attacaaaat
     5041 aaatagaaat aataatttat tcgtacaaga ttatggaatt aagcgtaaag gtaatttagt
     5101 tactgaagaa gcttacattc aaaacaatca gtatcaggat aaaatatatg aaaaattacc
     5161 ggatatagat tttagactcg aagattttta tagtaaggaa aactaaataa tataaagcac
     5221 cactaatcgt agttgatcaa ttacacatta gtggtgtttt attttgaatt taaatttgat
     5281 aaatgaataa gagtatacgc attcatttaa gattagatga cattgataag tatgagtaat
     5341 gtattaggta accattgaat tcgttgttat acgattctaa tacaagatta tgataaaata
     5401 gctttaatta agaattttag ccatcatata gtcatcgtaa tatttaccat cgatgaataa
     5461 cttatctttt aaaacgcctt cgatttgaaa atcggcactt ttaaaaagct ctagggcagg
     5521 ttggttattg agtggtacat ttgcttcaat tcggtgtatt tgattgttta aacaccaagc
     5581 cataatggca tcaagaagtg cttggccaat tccacgatgt tgatataatt tctttacacc
     5641 taaatcaatt ttagcaacat gtttaatgcg ttgaaatggt gtcgtattaa caaaggcaaa
     5701 gcctacaagt tgttcatcac tttcagcaac aaagatgact ttatgtggag aagtgatata
     5761 ttcttctaat tgtttactag ccgatgtgac gctaggatca tattctcctg gtgtgtagaa
     5821 catatacgga gattcgtcgt atattttcgc taacattgaa atgaaatttt ctacatcttt
     5881 gatactaact ctacgtataa tatgggccat aaaaagcctc cagtattttg aaattttaaa
     5941 acatttgcta ctattataat atatatttaa ccaaaaggta gagtaatatg ttttttggtt
     6001 acagtttaaa ggagtatttt agatgaaacc taaagtttta ttagcaggtg gaacagggta
     6061 tattggtaag tatttaagtg aagtgattga aaatgatgct gaactttttg ctatatcaaa
     6121 atatccagac aataaaaaaa cagatgatgt tgaaatgact tggattcagt gtgatatatt
     6181 tcattacgaa caggttgttg ccgcaatgaa tcaaatagat attgctgtat tctttatcga
     6241 tccaacaaag aattctgcca aaataacaca atcatcagca agagatttaa cattaatcgc
     6301 agcagataat tttggtcgag cagcggctat caatcaagta aaaaaagtaa tctacatacc
     6361 tgggagtcgt tatgataatg aaacaattga acgcctaggt gcatatggta caactgtaga
     6421 aacaacaaat ttagttttta aacgttcttt agttaatgta gaattacaag tttcaaagta
     6481 tgatgatgtt agatcaacga tgaaggtagt tttaccaaag ggatggacat taaagaacgt
     6541 tgtaaaccat tttattgcat ggatgggtta cactaaagga acttttgtga aaacagaaaa
     6601 atcacatgat caatttaaga tatatattaa gaataaggtg cgaccactcg cagtatttaa
     6661 aatagtagaa acagctgatg gaataataac tttaatttta ttgagtggaa gtttagtgaa
     6721 aaaatataca gttaatcaag ggaagttaga atttagatta atcaaagaat catcagtcgt
     6781 ttatatacat ctatacgatt atatccctcg attattttgg ccgatttatt actttataca
     6841 agcaccaatg caaaaaatga tgattcatgg ctttgaagtt gactgccgga ttaaagattt
     6901 tcaaagtcga ttaaaatcag gagaaaatat gaaatatact aaatgatatt gggtgatatg
     6961 gatgcaaata ctactagtag aagatgacaa tactttgttt caagaattga aaaaagaatt
     7021 agaacaatgg gattttaatg ttgctggtat tgaagatttc ggcaaagtaa tggatacatt
     7081 tgaaagtttt aatcctgaaa ttgttatatt ggatgttcaa ttacctaaat atgatgggtt
     7141 ttattggtgc agaaaaatga gagaagtttc caacgtacca atattatttt tatcatctcg
     7201 tgataatcca atggatcaag tgatgagtat ggaacttggc gcagatgatt atatgcaaaa
     7261 gcctttttat acaaatgtat taattgctaa attacaagcg atttatcgac gagtgtatga
     7321 atttactgct gaagaaaaac gcacgttaac atggcaagat gctgtcattg atctatcaaa
     7381 agatagtata caaaaaggtg atcagacgat ttttctatcc aaaacagaaa tgattatttt
     7441 agaaattctt attactaaaa aaaatcaaat cgtttctagg gatacaatta ttactgcgct
     7501 atgggatgat gaagcatttg tcagtgataa cacattaaca gttaatgtga accgattacg
     7561 taagaaacta tctgaaatag ggatggacag cgcaattgaa acaaaagtag gaaaaggata
     7621 tatggcacat gaataatttg aaatgggtag tttattttct gaaatcaaga aagaattgga
     7681 tattttggat actgttttta aatattctta tgttaggcat tagtcttatt gactatgact
     7741 ttccaatcga tagtttgttt tatattgttt ctttaaattt aagcttaaca ctgatttttc
     7801 ttatattgac tttttttaaa gaagtaaaat tatatagaca ttttgaaaaa gataaagaaa
     7861 tcgaagaaat aaaacataaa gatttagcgg aaaccccatt ccaacgtcat acagttgatt
     7921 atttatatcg tcaaatttta gcgcataaag ataaagtggt cgaccaacag ttgcaattaa
     7981 acatgcatga acaaaccatc acagaatttg tgcacgacat caaaacacca gtgactgcta
     8041 tgaaattgtt aattgaccaa gaagagaatc aagaaagaaa acaagcgcta ttatttgaat
     8101 ggtcacgtat taactcgatg ttagatacgc aattgtatat tacgagatta gagtcgcaac
     8161 gtaaagatat gttttttgat tatgtctcat tgaagcgcat ggttattgat gaaattcaat
     8221 taacaaggca tataagtcaa gttaaaggta tcggatttga tattgatttt aaagtggata
     8281 atcatgtgta cacggatata aaatggtgtc gtatgattat tagacagatt ttatcaaatg
     8341 ctttgaaata tagtgagaat tataatgttg atatttcaac ggaattaatt gatcaacatg
     8401 ttgcgttaat catcaaggat catggaagag gtattagtaa aaaagatatg cctcgaatat
     8461 ttgaacgtgg gtttacgtca acggctaaca gaaatgaaac gacatcatcc ggcatgggtc
     8521 tatacttagt ggatagtgtg aaagatcaat taggaataca attacaagtt acttcgacta
     8581 ttggaaaagg aacaacggtt aaattgatct tcccattaca aaatgaaatt gttgagcgca
     8641 tgtcagaagt gacaaatctg tcattttaaa catgcgtttt gttacttaga attgatacat
     8701 catgaccact tcaacgatat aatcaaatag atgttaatta aatacaaaat tgaaggtaag
     8761 cgtgccaaat tgaatataaa ggaaatgaag ttaagataaa tataggagtg ttaaagtgac
     8821 aatattagaa gtaaaacaat taacaaaaat atatggcaac aagaaaatgg cacaagaagt
     8881 tctgcgagat atcaatatgt ctgttgaaga aggtgaattt attgccatta tgggaccttc
     8941 aggatctggt aaaacgacct tattaaatgt tttgagttca attgattata tttcgcaagg
     9001 ttctatcaca ctaaaaggac acaaacttga aaagctttca aacaaagcat tatctgatat
     9061 tcgaaagcat gatattggtt ttatttttca agaatataat ttacttcata cattaactgt
     9121 taaagaaaat atcatgttac cattaacggt tcagaaatta gataaggaca caatgttaga
     9181 tcgttatgaa aaagtggcag aagcattaaa tatattagac atcagtgata aatatccatc
     9241 cgaattgtct ggtggtcaaa gacaacgaac atcagctgcc agagcattta taacattgcc
     9301 ttctattata tttgccgatg aacctacagg tgcactcgat tcgaaaagta cacaagattt
     9361 attaaagcga ttaatgaaaa tgaatgaaga gattaagacg acaattatta tggtgacaca
     9421 tgatccggtt gcagctagct atgcaaatcg agtagtgatg cttaaagatg ggcaaatatt
     9481 cacggagtta tatcaaggtg atgatgacaa acatactttc tttaaagaaa tcatacgtgt
     9541 acaaagtgtt ttaggtggca ttaattatga gttttaatga gattatattt aaaaatttcc
     9601 gccaaaattt atcacattat gccatttatc ttttttcgct aattacaagt gtagttttat
     9661 tttttagctt cgtagcacta aaatatacgc atagcattaa cataactgag acatatccca
     9721 ttattaaaga aggatcgcaa gtaggtagtt actttctatt ttttatcatt gttgcctttt
     9781 tgctatatgc caatgtacta tttcttaaac gtcgtagttc tgaacttgca ttatatcaaa
     9841 cattaggttt atccaaattc aacattatat atatattgat gtttgaacaa ttattaatat
     9901 tttcaataac agctatattg ggcattacga ttggtatctt tggttctaaa attttattaa
     9961 tgatagtgtt tactttatta ggtatcaaag aaaaggttcc aattattttc agctttagag
    10021 cagtgtttga gacactatta ttaattggcg ttgcttattt cctaactgca gctcaaaatc
    10081 ttatcttagt attgaaacaa tctatttcag aactatcaaa gcataatcag gctaagcaaa
    10141 tgaatcataa taaaattaca tttgaagaag ttatactagg aattctaggt attattttaa
    10201 tcatcagtgg gtattactac tctttacgca ttgtacatta ctatgatgca attggcgtcc
    10261 ttatgtttat attgtttgcg acagtaattg gggcgtattt attttttaaa agttcagtat
    10321 ccttggtatt taaaattatt aaaagcttta aaaaaggtgt aataagtgta aatgatgtca
    10381 tgttttcttc atctattatg tatcgtattc agaaaaatgc attttcactt actgttatgg
    10441 caattatttc agctattact ttatcagtac tttgttttgc tgctatcagt agagcaactt
    10501 tattaagtga aattaagtat acgtcacctc atgatgttac gattaaagat caacagaaag
    10561 cgaatcagtt agcaagtgaa ttgaacaacc gaaaaattcc tcatttttat aattataaag
    10621 aagtgattca tacaaaactt tataaggatg acttatttga tataaaagca aaggaaccat
    10681 ataacgtaac aattactagt gacaaatata ttcctaatac ggatttgaaa cgtggtcagg
    10741 ctgatttatt tgtagcggaa ggcgctatta aagatttagt gaagcataag aaacatggta
    10801 aggcagtaat aggaacgaaa aagcaccatg tggatattaa attacgtaaa gatatcaata
    10861 aaatttactt tatgactgat gttgacttag gtggaccgac gtttgtgtta aacgatcaag
    10921 attatcaaga gataagaaag tatactaagc caaaacacat tgtttctcaa tttggatttg
    10981 atttaaaaca taaaaaagaa atgttagcat tggaaaaagc aacaaataaa gttgacaaat
    11041 ctattgaaac aagaagtgaa gccataagtt cgatatcgag tttaacggga atattgttat
    11101 ttgtaacctc atttttagga attagtttcc taattgccgt atgttgtatc atatacatta
    11161 aacaaataga tgaaactgaa gacgagttag aaaattatag tattcttaga aaacttggat
    11221 ttacacaaaa agatatggca aaaggattaa aatttaaaat tgcgtttaat tttgggttac
    11281 cattagtcat tgcactttca catgcatact ttacatcatt agcatatatg aaattgatgg
    11341 ggacaacgaa tcaaataccg atatttatag taatgggact atatatttgt atgtatgcca
    11401 tttttgcaat gacagcttat aatcattcta agcgtacaat cagacattca atttaatata
    11461 tatatagcgg ctttcagtag gctagtggat taagattcat gagctttact gaaagctttt
    11521 atttttggat agttataggt ccgggcacga cagttttatt gtatatgctt caactttgta
    11581 aattgtatta aatgtgagaa aatagaggca taggcattga aagaaatatg aattaagaat
    11641 agttagaaga aagataagtg tattttaata tgtaaaagtt atgtaataaa tattgtatcg
    11701 ttgcaaattt tccgagtatg tccatttaca attattagag ttttgtagat aaaaaattgc
    11761 ctatatatca agtttttcaa aacaaatgcc taccactatt taaattttat gtaaaagtta
    11821 agtttcggaa ttgttaaaaa tttgtaaatt gaattgtggg attgtaaatt ttgtgctatt
    11881 ttcatataag ggcaaataca gtaaattgtt tactgtgagg catttttgaa attaatatca
    11941 gtacactaaa aatatactga cgattgataa taacaaattt gtatcattag tttgtaaatt
    12001 cacttgtctt aatttgaaac aaattataac tgaatgtgat tggtgacaat cgcttaaatg
    12061 gaggatttta aatgtttagt aagaaaaaag ataagtttat ggttcaatta gaagagatgg
    12121 tttttaacct agatcgtgca gctattgaat tcggtaaaat ggatttcaat acacatctag
    12181 atttaaaggc atactcagac aacattaaaa cttatgagtc acatggtgac gaattagtac
    12241 atcaagtaat tactgattta aatcaaacat ttatcacacc aattgaacgt gaagatattt
    12301 tatcattatg tgatgcaatt gatgatgttt tagatgcaat tgaagaaacg gcagctatgt
    12361 ttgaaatgta ttcaatcgaa tacacagatg aatatatggc tgagtttgtt gataacattc
    12421 aaaaagcagt tgcagaaatg aaacttgctg tcggcttatt agtcgataaa aaattatcac
    12481 atatgcgtat tcattcaatt aatattaaag aatttgaaac aaactgtgat ggtattttaa
    12541 gacagtcaat taaacatatt ttcaatagtg aaacagatcc aatcacatta attaaaataa
    12601 aagatattta tgaaagcatg gaagaaattg ctgataaatg tcaaatcgta gcaaataatt
    12661 ttgaaactat tattatgaaa aatagctaag gggagtatat atttatgtca tatataatca
    12721 tcgtcactat agctgtagtt attttctcgc tgatatttga ctttatcaat ggattccatg
    12781 atacagccaa tgcagtagct actgctgtat ctactagagc gttaacgcct aaaacggcaa
    12841 ttttaatggc agcagtgatg aactttatag gtgctttaac atttacgggc gttgcaggca
    12901 ccattactaa agacattgtc gatccattta aattggaaaa tggattagtt gttgtgttag
    12961 ctgcaatact tgcggctatt atttggaatt tagctacttg gttttacgga attccaagtt
    13021 cgtcttcaca tgcacttata ggttcaattg cgggtgcagc aatcgcatct gaaggctcat
    13081 ttggcgtatt acattaccaa ggtttcacaa aaattattat tgtattaatc gtttcaccga
    13141 ttatcgcatt ttgtgttggt ttcttgatgt attcaatttt taaagttatc tttaaaaatg
    13201 caaatttaac aagagcgaat cgtaacttta gatttttcca aattttcaca gcagcgttac
    13261 aatcattctc tcacggtacg aatgatgcgc aaaaatcaat gggtattatt acgttggcat
    13321 tgattgttgc taatgtacag aatgatggca gcgttgaacc acagttatgg gtaaaatttg
    13381 cctgtgcgac agcaatgggg cttggtactg caattggtgg ctggaaaatt atcaaaactg
    13441 taggtggtaa tattatgaaa atacgcccag caaacggtgc tgcggccgat ttatcatctg
    13501 cattaacaat ttttgttgca tcatcgctac atttcccatt atcaacaact cacgttgtgt
    13561 catcatcaat cttaggtgtt ggtgcttcta accgagctaa aggtgtaaaa tggagtactg
    13621 cgcaacgaat gatcattaca tgggtgatta cattacctat ttcagcattg ttagcaggtt
    13681 tattattcta tatacttaat ttatttttct aattgaaaat taagctatac tgaacttcag
    13741 tatcacaaat atatggtggt attgaagttc atttttttat gtaaatagac aacctatttt
    13801 atagagttta catagaatga ccaagacaaa gcattttaat taggggctaa gttataaatg
    13861 tattccatgg aacataataa gttaggtcat taaaatataa gtaagcgtaa aataatgatt
    13921 taacagttta tattcttcaa tgaatgaata attaaatagc tatcaacttg tttaattaac
    13981 ctaaattgta ttggacttta tttaatgtaa atttgtatat gtagacgaaa aagcgtaaaa
    14041 tactggtata acaaattaaa tcattatata gtcaacttgg ttgcattatt ttggttttta
    14101 atatattatg catttaattt aatcaaatag gtaggttatt attttttacc taactaccat
    14161 tcatacaaaa tataaataag tatgataggg aagggtgaga taattttttg taccaaaatg
    14221 aaaatttttt tcaaaaataa aaaactagca atctcacatg atgtgaattg ctagtatata
    14281 tcagtacaat ttatttaatt aatggatgaa tgcatagcta gaaacttctg aagctggaat
    14341 tgtacggtag ttcatattgt atggaccata tgtgtaattc atttcagaaa tcaagatact
    14401 accatcacca ttgacacgtt caacataagc aacatgacca tatggaccag gtgtgctttg
    14461 catgattgaa ccaactgatg gtgtgttgtt tacttggtaa ccatcattag ctgcgttacc
    14521 agcccaatac ttagcgtctg accaatatgt gctaattgga ctaccagctt gagcacgacg
    14581 gtcaaatacg taccatgtac attgaccagc agtgtataaa ttttggtgat taaaagatga
    14641 tgcattgcca ttgctacctg ttgtagctgt tggtgttgta ccacctgatc caccattagg
    14701 aatttgtaat gtttggttag gcataattaa ataaccacgt aagttattgg ctgccattaa
    14761 ttgatcaact gaaacaccat atctgttagc gatgatattt aatgattcac cagcttgtac
    14821 agtatgagat gatgctgaac cagcttgtgg agaagtgttt gacgtatttt gtgcatcact
    14881 tccacctact gagataactt gaccagggaa taccaagttg ttatctaatt ggttattttg
    14941 tttaatactc tctactgaag tgttgtattt ttgagcaata ctccataatg attcaccaga
    15001 ttgtactgta tgttgtgtag aagcttgcgc atcatgatgc gttaaaaatg cagctgcacc
    15061 agatgttgct gttattgcaa atgctaattt tttcaaaggg actcctcctt aaaattaaga
    15121 tttactactt ttaaaaatta ttaagacaat ttatatagta aattaagttt tcgttttata
    15181 ttacgctttc catattatca aaaataagta gctttgtgtg gtttgttatt atcttgtaat
    15241 attattaaca ttttaggata tcggttcata tttttataaa tgctgatttg atagtgtata
    15301 aaagcgtttt agtgatttta actaattata aaaaattata ttttgtaaca ttataaaatg
    15361 taaaattact atacttgtta aaggttattt cttttgatga agcaaaattt actgtaaaat
    15421 ttgacgcata aaacgacagt agaaggagga attattttgt cgcaaaatac aaatcattca
    15481 tattatcatc aaaaccagca tgctcaagca ataagtaaag tgtggcttta ttttatgtat
    15541 tattggatta tatttggcat aggatgctat ctaggtcagt ttttaccatt aagttggcga
    15601 caacccttgt catttggatt actgattatt attttagcaa cacttgtttt tgaaagagcg
    15661 agacggttcg gtttaattat ttcacatata tacgctgtag tgattggctt attgtcatac
    15721 gcaacgttta ccacgtattt acaaaattta ggaccagata ttttctataa aaatatcgca
    15781 ttagcaattt ttgcatttat agcatttggt attattggtt atttcttcgt tggagatgca
    15841 tcgagtatag gcaagtattt attcgttaca ttaataacat taattattgc gagtctaatt
    15901 ggtatttttc ttcaaaatcc tattttttat actattatta ccgtcgttag tctgttgtta
    15961 tttctacttt atactttgta tgattttaat cgtttaaaaa gaggtgacta ttcaccaaga
    16021 gaaatgggat ttaatctatt tattaatttg ttgaatatta ttaaggatat actttatctt
    16081 gctaatatgt tcagaagata aacagttaaa ttaataaaga aattcatata aagttttaga
    16141 ataatcaata atgaatatca actttaaatt ggagcgattg tcataaatta gaaatttaga
    16201 tagggtaatt taaatatatt accttaattg taaagatatt attaatcatt gaaatgatta
    16261 actgctatag ttctaaagta aacaaatctt agaagataaa ctttaaacag gtgtacttgc
    16321 ctttctaatt acacttttat tagaaaagta aagtatagct acgtattaat tttaaaatcg
    16381 aaatattaat aggaataagt atagataata taaacaattt aatgttagtt tgagctcatc
    16441 tcaaaagtag aatgtgattt aaataagtgt ttatttttaa attgaaaatg aatgtaaagc
    16501 taacaaaaga taatagtaat acgttgtaga tgtggtcatt taaaattatt ttgaaaatac
    16561 taaaaaatag tgtgttttca attggatagt ttaattaaac tgactacatc tatttttgta
    16621 atatttaaat gattgatata taagtttaaa aattacccat tatatagttt tatttatttt
    16681 tgatttggta ttcgtattgt gttaaattta cgtttaatta aataagttaa atattatagg
    16741 gaatgataaa agtggtactt aaatatagaa agaggtaaag ttatggcaaa atcatgcttg
    16801 catatactta ctaataatga atatgcgaca acgcgttgcc aagatggcat agtcttattt
    16861 tggccaattg acggggaaat cgaactacaa aaatttcgta aaagtaaaat aattgaagat
    16921 gatatatata tattattaat catctggatg tatttagtat taagaataat aaaaaaacga
    16981 tcatgttgta tttgagtagc gattggtttg cggaattagg ctttactttc tttaattacc
    17041 actatacagc aaagttaatt aaatcatctt ataatttgaa atgtttacta ttaaaattga
    17101 cttatcgata ccttgataat caacctctca atgacgctga tgttagaaaa ttacaggata
    17161 ttattaaaat tattgcaaaa gaagcaagta tggataaaaa gattgcgcaa aatcaatatc
    17221 gatatgccta ttatggtgat ttgcgtgacg agctcgaata tatttatcaa aatgtaaatc
    17281 aacgattgac attaaaaagt gtcgctgata aattatttgt ctcaaagtca aatttgtcat
    17341 cacaattcca cttacttatg ggcatgggtt ttaaaaaata tattgatact ttgaaaattg
    17401 gtaaatcgat tgaaattcta cttactactg atagtactat aagcaacata agtgaacatt
    17461 taggttttag tagtagctca acttactcta aaatgtttaa aagttatatg gatataacac
    17521 cgaatgaata tcgtaattta tcaaaattta ataaatctat aatgcttaat ccagagccac
    17581 ttgtaggcgg tatggcgcaa gaagtaaaag aagtcatatt gaattatatt gatcattata
    17641 aaaatcacct aactgatgtt atacatattg acgaagacaa atttgaaata cctaagttat
    17701 ttcaaacggt tattcaaatt aatacttata cagaaatgaa actagttttc ttagaaggac
    17761 tttttaaaac cttattgaat aagaatagcc aagttgtctt ttttatcatg ccatcgattt
    17821 taaaaagtaa aaataccatg tccgaagaag aaaagtttac aatcattaaa accataattg
    17881 aaagtaattt aaagatagca tttaatataa atgatattga aacgacttat tatgttgaag
    17941 aagcttttat gaatgttatt agacaattat ctccaaacga aataaatcat cataataatt
    18001 acgaagtgca ttttgttttt gatttatcat tgatggaaat taggacaatt taccgtatga
    18061 ttttaaaact acataacatc atgttaaatg tgaaattagg attgaatatt acctgtttat
    18121 ttgataaacc ttcagttttt aaatcactag tatcacaaat aaagcgactt aaattcgatt
    18181 cgttaataat agataatgca aatttaagta gcccttattt gatgggggaa agtgatgagt
    18241 tactattgaa aaatattttg cattttaaaa atttaaaaca agttattaac gaattggata
    18301 tagaacaaga aaagctgatt tttctaaatg ttgaaaatca taaactgctt aataataaag
    18361 aacgaaattt aagtaatagt gctccattaa tttataagac attaagtgcg ctgtatcata
    18421 actttcatgg ttttggatta aacatttttg ataatcatca aacatttaat gcgatgcatc
    18481 tatatgataa aaatggattt aaaacaacac taggtcttat attagaaaag tttatcgaat
    18541 atgtctcgaa accaaaatac gaaaacagtt attattctat ttttgatata gagaattatt
    18601 attgtctggt tatttatgat tggcgagtga tagagagcga gacaattatg agcaattttg
    18661 aagatagtca agtttatatt aatttcaaaa ataatatttt aaacgataaa tatctaattg
    18721 taatagaaac gttggatgaa aatagtggga acattaatca tttgatctct aaagaattaa
    18781 gagataaata tgaatggaac cccagtttac tctctaaaat tgacaactac cttaaaccgg
    18841 caatcgagat taaagagcat aactttagta ataattcctt aaatattaac gttactttta
    18901 atgcgttata cataattaaa ataggaaaaa aataacacct tgatatgtat tgcaaaattt
    18961 aatttgcatt tgttggtaat tttgacaacg tattaaaaac aaatgagaaa gacaatggca
    19021 gaaaaattta agataataat gacagaagca ttgtctttat atatttgggg gtgcaacatt
    19081 ttgaatactg agaaattaga aacattgctt ggcttctata aacaatataa agcattatct
    19141 gaatatattg ataaaaaata taagttgtcg ctaaatgatt tagcagtctt agatttaacg
    19201 atgaagcatt gcaaagatga gaaagtactt atgcaatcat ttttaaaaac tgcaatggat
    19261 gagctagatt taagtaggac aaaattatta gtttctataa gaagactaat tgaaaaagaa
    19321 agacttagta aagttagatc atctaaagat gagcgtaaaa tttatattta tttaaatgat
    19381 gatgatatat ctaaatttaa tgctttattt gaagatgtag aacaattttt aaatatttaa
    19441 ttgaaattga gtgtcgaaag cgtagaaatt gcttatcggc acatttttaa tttatacata
    19501 ttttaaaact aagtaacaga ttgaagaaat cgtagttcaa taatgttaat tgtgaaaatg
    19561 tatataatca taaaaaaatc atgtataata tatgttgtta attaaacagt tcgaaagcga
    19621 gatgacatta tgggacgtaa atggaataac attaaagaaa aaaaggccca aaaagataaa
    19681 aatacaagta gaatatatgc gaaatttggt aaggagattt atgttgcagc aaaatctggt
    19741 gaaccaaatc cagaatctaa ccaagcttta agattggtac ttgaacgcgc taagacatat
    19801 tcagtaccga atcatattat tgaaaaagca atagataaag ctaagggtgc tggagacgaa
    19861 aactttgatc acctaagata tgaaggattt ggcccaagcg gatcaatgct tattgttgat
    19921 gcgttaacaa acaatgtaaa tcgtactgca tctgatgtgc gtgctgcttt tggtaaaaac
    19981 ggcggtaata tgggtgtatc tgggtcagtt gcttatatgt ttgaccatgt ggcaacattt
    20041 ggtattgaag gtaaatctgt tgacgaaata cttgaaacat taatggaaca agatgtagat
    20101 gtaaatgatg tgattgacga taatggattg acaatagtct atgctgaacc agatcaattt
    20161 gcagtcgttc aagatgcgct tcgtgcagca ggtgttgaag aatttaaagt tgctgaattt
    20221 gaaatgttac ctcaaacaga tattgagctt tctgaagcgg accaagtaac atttgaaaaa
    20281 ttaatcgatg cattagaaga tttagaagat gtacaaaacg tattccataa tgtggatttg
    20341 aaataatgaa atcagcagaa caatggattg atgaattgca acttgaatcg catcctgaag
    20401 gtggtttcta tagagagaca atccgagaag tattgaaaga tggacgaaga gcgccgttta
    20461 gtagtattta ttttttgctt acagatgaca atatttcgca ttttcatcga attgatgctg
    20521 atgaagtatg gtactatcat gctggtgatt ctctaacaat tcatatgata aatccggatg
    20581 gggaatatac gactgcaaca ttgggtactg atatccaaaa tggagatgta ttgcaatatg
    20641 cagtgcctaa aggaacaatt tttgcttctt caatcgaaat ttcaaatact tatagtttag
    20701 taggttgtat gtgtcaacca gcatttgagt ttaagcagtt tgaattgttt aagcaatctg
    20761 aattaattac acaatatccg catcttaaat cagtaatcga aaaatatgct ttaaaataaa
    20821 aagaatcaat gaagtgattt gaaggttgtt agtaaacctt tgagtcactt catttttgta
    20881 tgtattcttg attgaatcag aatagatttg gtgcttcagc tgattttaat gaaaatagca
    20941 ttaaatgatt ttgaaaaaga taagagtgtg ttatttatat ttttgaaaaa tcacttttat
    21001 gaagaaatgt gtgtactatc taattaatat tgtctatttt aagtaaattg aagcgagttg
    21061 tagtaaaata ataataaata ttttcatatg attaacaaaa actataaaac tgtatcatga
    21121 catgacatca tccatgtagt tttatataat aatgacaagg agttggtgac tgtgaaagta
    21181 aagtatatag ataaacgtca ctggcgtcgc ctaattgata gggaatacac agaggtaaaa
    21241 gttaataata ataggttcaa gggtattata ggcttagtca cgatgaaaaa ggttcgtgat
    21301 cctttagagg tgacggtagt tggacaaaat attattgtcg cagatgacaa ttataaatgg
    21361 ttgcaaatac tacctgaaaa gaaacgttat agtattactg taatgtttga taataaaggc
    21421 aatccattag aatattactt tgatataaat attaaaaata taacgcaaaa aggtaatgcg
    21481 cgtacagtag atttatgttt agatgtttta gcattaccta gtggtgaata tgagttggta
    21541 gatgaagatg atttaatgtt tgcattagaa agtgagcaaa ttacaaaaaa gcaatttcat
    21601 gaagcatata tgattgcgca tcaaattatg gcagagttag aaaatgattt taaaggattc
    21661 caaaagaaaa tcatgtactg ctttaataaa attaatgcaa aggctcaaaa aattcatcaa
    21721 aagccacaaa ataaaactaa ttttgaaaaa agcaaacaaa taaaacctaa gcaatataat
    21781 caaactaaaa atcaccaaca acaaaagaaa aactaagtaa ttcaagctgc agccatacca
    21841 ataaaattgg tgaaaattat tcgttagtaa tgaactcttg aatgctcgta gtcatgaaag
    21901 ttcaataatt gaataattta tgggggggaa attatgaaga ttgaagacta tcgtttacta
    21961 ataacattag acgaaacgaa aacgttacgt aaagcggctg aaattttata tatatctcaa
    22021 cctgctgtta cacaaagact aaaagctatt gaaaatgctt ttggagtaga tatttttatc
    22081 agaacaaaaa aacaattgat tacaacaact gaaggaacaa tgattattga gcatgcccgt
    22141 gacatgttga aaagagagcg attatttttt gacaaaatgc aggcacatat tggtgaagtg
    22201 aatggaacga tatcaatcgg gtgttcttca ttgattggac aaaccttact tcctgaagtt
    22261 ttgagcctat ataatgccca atttcctaat gttgaaattc aagtacaagt tggttcaact
    22321 gaacaaatta aagcaaatca tagagattat catgttatga taactcgtgg aaataaggta
    22381 atgaatttag ctaatacaca tttatttaat gatgatcatt attttatttt tccgaaaaat
    22441 agacgagatg atgttacaaa gttaccattt atagagtttc aagctgatcc gatttatata
    22501 aatcaaataa aacaatggta taacgataat ttagaacaag attaccatgc aactattaca
    22561 gtggatcaag tagcaacttg caaagaaatg ttgattagtg gtgtaggtgt tacaatcttg
    22621 ccagaaatca tgatgaaaaa tatcagcaaa gaacaatttg agtttgaaaa agtagaaatt
    22681 gataatgaac cgctgattcg ttcgacattt atgagttatg atccgagtat gttgcaatta
    22741 ccacaagttg attcatttgt aaatcttatg acgagctttg ttgaagaacc aaaggcgtag
    22801 tcttagacta atttaaggtt agtatttaat tttaagatat acggttaaat tgaacacagt
    22861 tggatgctaa tgcaccaaca gcaaaagagc ccctaattaa taaattaaaa ggggctctaa
    22921 gaagcggggt tatggatatg tttgcagcgt tattacaaat aaagaattat aaactctttg
    22981 ttgctaatat gtttctatta ggtatgggta ttgcggttac ggtcccatat cttgttcttt
    23041 ttgcaactaa agatttgggt atgacaacaa atcagtatgg attacttcta gcatctgcag
    23101 cgattagtca gtttacagta aattcaatta ttgctagatt ttcggatacg catcacttta
    23161 atagaaaaat tattattatt ctcgcattat taatgggtgc gcttggtttt tcaatatact
    23221 tttttgtaga tacaatctgg ttattcatat tactatatgc gattttccaa ggattatttg
    23281 caccagcaat gccccaactt tacgcatctg ctagagaatc tatcaatgtt tcaagctcta
    23341 aagatagagc ccaatttgcc aatacagtat tacgttcaat gttctcattg ggctttttat
    23401 ttggtccatt tattggtgcg caattaatcg gattaaaagg ctatgctgga ttgtttggtg
    23461 gaacaataag tatcatttta tttactttag tacttcaagt gtttttctat aaggatttaa
    23521 acattaaaca ccctattagt acgcaacaac atgttgaaaa gattgctcct aatatgttta
    23581 aagacaaaac gcttttatta ccatttattg catttatttt attacacatt ggacaatgga
    23641 tgtatacgat gaatatgcct ttatttgtta ctgattattt aaaagaaaat gagcaacatg
    23701 tcggttattt agctagttta tgtgctggtt tagaagtgcc atttatgatc attcttggcg
    23761 ttttatcatc tagattacag actcgaacat tgttgattta tggagcgatt tttggaggtt
    23821 tattctactt cagcattggg gtatttaaaa acttctatat gatgttagca ggacaggtgt
    23881 ttttagctat tttcttagcg gttcttttag gaattggtat tagttatttc caagatatct
    23941 taccagattt cccaggatac gcctcaacac tattttctaa tgcaatggtt attggacagt
    24001 taggcggtaa cttattaggt ggtgctatga gtcactgggt aggtttggaa aatgtatttt
    24061 ttgtatcagc agcatcaatc atgttaggta tgatacttat attctttact aaaaatcaaa
    24121 aaattacaaa agaggatgtg atatcaacat gacaattgtt ttatggctac ttatcatcgc
    24181 tgccttcatg ttagcatttg ttgggttgat taagccgatt attccttctg ttttagtatt
    24241 atgggttggc tttctaatct atcaatttgg ctttcataat cagcatttat catgggtgtt
    24301 ttatgtatct atggcattgc taacaatatt aattttatgt gcggactttt tagctaataa
    24361 atattttgtg aatcgcttcg gtggttctaa gtttggagag tatgcagctt taattggtgt
    24421 cgttattgga tgttttgttt taccgccatt tggaattatt atcattccat ttattttggt
    24481 attcatagtt gaattaatac aaggctattc atttgaaaga gcagttaaag taagtatagg
    24541 ttcaatcgta gcatttttaa caagtagtat agctcaag
//
LOCUS       DAMZPP010000021        24319 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMZPP010000021 DAMZPP010000000
VERSION     DAMZPP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24319
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.021"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..309
                     /locus_tag="POH73_000659"
     CDS             <1..309
                     /locus_tag="POH73_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDI6886634.1"
                     /translation="QRWANERQYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAY
                     IGNKDLVHSIEKKVDVISYGPSQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN
                     "
     gene            430..984
                     /locus_tag="POH73_000660"
     CDS             430..984
                     /locus_tag="POH73_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDI6886635.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSTGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGLK"
     gene            1056..1826
                     /locus_tag="POH73_000661"
     CDS             1056..1826
                     /locus_tag="POH73_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6886636.1"
                     /translation="MSHISMSDINCDGTIFENNDKQLIYLTPIFPQRYTSNTWLYKKM
                     PTQEQWLKDLKRQHQLHTNQGSNHYAFSFPENEQLDDTWMAIFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGRDYADAHEKMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTMQSLVGEYALSRNH
                     KPVILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(1997..2794)
                     /gene="fdhD"
                     /locus_tag="POH73_000662"
     CDS             complement(1997..2794)
                     /gene="fdhD"
                     /locus_tag="POH73_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDI6886637.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAVAKTSMSKITLTPQQIINMMTRLQSASHIYQETGGLHNAA
                     LSDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            3084..3866
                     /gene="modA"
                     /locus_tag="POH73_000663"
     CDS             3084..3866
                     /gene="modA"
                     /locus_tag="POH73_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6886638.1"
                     /translation="MKMKRFIAIVMALFLVLAGCSNSNDNQDNKKDAADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKGNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFTA"
     gene            3880..4551
                     /gene="modB"
                     /locus_tag="POH73_000664"
     CDS             3880..4551
                     /gene="modB"
                     /locus_tag="POH73_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDI6886639.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTILGIFISKWLYRRKGSWVKVLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGLRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            4552..5157
                     /locus_tag="POH73_000665"
     CDS             4552..5157
                     /locus_tag="POH73_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6886640.1"
                     /translation="MLKINLKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            5325..6329
                     /locus_tag="POH73_000666"
     CDS             5325..6329
                     /locus_tag="POH73_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDI6886641.1"
                     /translation="MSQERYSRQILFKQIGEIGQNKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IDHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKHRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRTACTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENTSITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            6358..6864
                     /locus_tag="POH73_000667"
     CDS             6358..6864
                     /locus_tag="POH73_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HDI6886642.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRNFDTDKGGQCVLQLLQVDNV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDVDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     EGFGELFRYLSYVEDVGTRALLSRAVAGTVNDKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(6866..7360)
                     /gene="moaC"
                     /locus_tag="POH73_000668"
     CDS             complement(6866..7360)
                     /gene="moaC"
                     /locus_tag="POH73_000668"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDI6886643.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIYKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTIIPMCHPLPLTGIDVHFNWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QLDQ"
     gene            7423..8682
                     /locus_tag="POH73_000669"
     CDS             7423..8682
                     /locus_tag="POH73_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDI6886644.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSTMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETTTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQQDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFADGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            8679..9164
                     /gene="mobB"
                     /locus_tag="POH73_000670"
     CDS             8679..9164
                     /gene="mobB"
                     /locus_tag="POH73_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDI6886645.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFKAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLTNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            9178..9624
                     /locus_tag="POH73_000671"
     CDS             9178..9624
                     /locus_tag="POH73_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HDI6886646.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            9630..9863
                     /gene="moaD"
                     /locus_tag="POH73_000672"
     CDS             9630..9863
                     /gene="moaD"
                     /locus_tag="POH73_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HDI6886647.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFENLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            9870..10469
                     /gene="mobA"
                     /locus_tag="POH73_000673"
     CDS             9870..10469
                     /gene="mobA"
                     /locus_tag="POH73_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDI6886648.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            10489..11511
                     /gene="moaA"
                     /locus_tag="POH73_000674"
     CDS             10489..11511
                     /gene="moaA"
                     /locus_tag="POH73_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDI6886649.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATADGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(11792..11962)
                     /locus_tag="POH73_000675"
     CDS             complement(11792..11962)
                     /locus_tag="POH73_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886650.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNDLECDNNVKEPLKIYYV
                     VILKSGIEMIFS"
     gene            12088..12438
                     /gene="sarV"
                     /locus_tag="POH73_000676"
     CDS             12088..12438
                     /gene="sarV"
                     /locus_tag="POH73_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HDI6886651.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(12540..13751)
                     /locus_tag="POH73_000677"
     CDS             complement(12540..13751)
                     /locus_tag="POH73_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6886652.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFFVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGVIISLFIKVDFEVTNSKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFAIERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITILVYFLI
                     HGRHVTRNAMN"
     gene            complement(13744..14184)
                     /locus_tag="POH73_000678"
     CDS             complement(13744..14184)
                     /locus_tag="POH73_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HDI6886653.1"
                     /translation="MLSQEFFNSFITIYRPYLKLTEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLKEAHND"
     gene            14347..15111
                     /locus_tag="POH73_000679"
     CDS             14347..15111
                     /locus_tag="POH73_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6886654.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(15103..15354)
                     /locus_tag="POH73_000680"
     CDS             complement(15103..15354)
                     /locus_tag="POH73_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886655.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIALAIIHMGILAHSTYVYLY"
     gene            15743..17008
                     /gene="femX"
                     /locus_tag="POH73_000681"
                     /gene_synonym="fmhB"
     CDS             15743..17008
                     /gene="femX"
                     /locus_tag="POH73_000681"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HDI6886656.1"
                     /translation="MEKMHITNQEHDAFVKSNPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEIQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMETSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            17125..20292
                     /locus_tag="POH73_000682"
     CDS             17125..20292
                     /locus_tag="POH73_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDI6886657.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTSMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKTSASNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVTTIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKIDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(20479..20796)
                     /gene="mspA"
                     /locus_tag="POH73_000683"
     CDS             complement(20479..20796)
                     /gene="mspA"
                     /locus_tag="POH73_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HDI6886658.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(21041..21205)
                     /locus_tag="POH73_000684"
     CDS             complement(21041..21205)
                     /locus_tag="POH73_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886659.1"
                     /translation="MSLENQLAELKYDYVRLQGDLEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            21338..22246
                     /locus_tag="POH73_000685"
     CDS             21338..22246
                     /locus_tag="POH73_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDI6886660.1"
                     /translation="MTEQFTMIILLIALGYFLKRINYIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSLSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFGMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVHVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     PFIGMTTNLTIIISIIILYVYQAVFV"
     gene            22417..22557
                     /locus_tag="POH73_000686"
     CDS             22417..22557
                     /locus_tag="POH73_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886661.1"
                     /translation="MYGAYLINEYHQDFKSIANFKVLNRMINVMMCYCVLFLEPCISW
                     DI"
     gene            complement(22665..23528)
                     /locus_tag="POH73_000687"
     CDS             complement(22665..23528)
                     /locus_tag="POH73_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HDI6886662.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWVGIIII
                     VIAAIILGNLK"
     gene            complement(23709..>24319)
                     /locus_tag="POH73_000688"
     CDS             complement(23709..>24319)
                     /locus_tag="POH73_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6886663.1"
                     /translation="LFKTMTSNQPDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYI
                     EARTRLEEDMHQPNIIPYHKGFYRAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFV
                     SEGLLKGYLYLEIDSQQSIAEIKYFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVY
                     FKIRNKNNKLIERFNGLGFHINYEYIKFKFESRNVKDQTIPE"
BASE COUNT         8722 a         3472 c         4514 g         7611 t
ORIGIN      
        1 caaagatggg cgaatgaacg ccaatatacg ggcattgatt ttttaggagt aagttatgca
       61 gcggtaccac cattaacaca ctttataaca aattctactt actttttatg ggatgtttta
      121 acgactgctt atattggtaa caaggactta gttcactcaa ttgagaaaaa agtggatgta
      181 ataagttatg gaccaagtca aggtaagaca tttgagtgta aagatgggcg caaaattaat
      241 gtcataaatc atgtagataa caacgcattt tttgattata taactgcact tgctaaaaaa
      301 gtaaattaac agctgtgtag aataattaag gttttaattt atatagaaca acttattgta
      361 aacttttcgt tccttaaagt ttacaatggt gctataataa tggtcatgaa atacgaaagg
      421 aagtaaaaaa tgacaacaaa acagttagta tacacagctt taatgacagc gattatcgct
      481 attttaggat tggtaccggt aattccacta ccattttctt cagtaccaat tgtacttcaa
      541 aacattggta ttttcttagc aggtgtgatt ttaggacgta aatatggcac attaagtgtt
      601 atcgtctttt tattattagt agttgctggt ttgccactgt tatcaggtgg tcgcggtggc
      661 attggtgtat tcgcaggtcc ttcaacaggg tttttactat tatatccagt tgtagcattc
      721 atgattggtg cgattcgtga tagattcatc aatgaaatta atttctggat tttattcgtt
      781 ggtattttag tttttggtgt tatagcatta gatgttattg gtacattgat tatgggcatg
      841 attattaaca taccatttac gaaagctatt tcaatttcat tagcttattt gcctggtgat
      901 atattaaaag caattgtagc aagtttgatt ggtacagctt tacttaatca ctcgcagttt
      961 cgtcaaatta tgggattaaa gtaatcatat ttaagataaa aaagtaattg attaagttgc
     1021 ttttcaattg ttgaaagtga aaggagtagg tgccaatgtc tcatataagt atgtcagata
     1081 ttaattgcga cggtacaata tttgaaaata acgacaagca gttgatttat ttaacgccta
     1141 tttttccaca acgatacaca agcaacacat ggttatataa aaagatgcct acccaagagc
     1201 aatggttgaa agacttaaag cgtcaacatc aattacatac aaatcaaggt tcaaatcatt
     1261 atgcatttag ttttccggaa aatgaacaac ttgatgacac ttggatggct atttttaaag
     1321 atatgaattt tgaactaggt attatggaat tgtatgccat agaaagtgat gcgcttgcta
     1381 atttgccgcg taactctgac gttgaaattg ctatcgttga cgagtcgcat atagatgcct
     1441 atttaaaagt tgcatatcag tttagtttgc catttggaag agactatgca gatgcacatg
     1501 aaaaaatggt aagggaacat tatcaaaaag atgtgattaa acgcttagta gcttatttaa
     1561 ataatgaacc tattggcgtt gtagatgtca ttgaaagtga aaattacatt gaattagatg
     1621 gatttggtgt actagaacaa tttcgacacc aaggaatcgg ttctaccatg cagtcattag
     1681 taggtgaata tgctttatct agaaatcata aaccggttat attagttgca gatggcgaag
     1741 atacagcaaa agatatgtat gcaaagcaag gttatgttta ccaatcattt tgttatcaaa
     1801 tattaaaaga agatattgga aattaacgat tgatgatagg tttgtattag aataaaaaag
     1861 cagtaaactg gtttcgagta gaaatcaact tactgctttt taaattgttt tgagctactt
     1921 atacttataa aaatagtgct tttatattgt tgattcatgt agaatacgtt cattatgaca
     1981 tactataatg aatatgttat tgttcagaat caatgatacg ttctggatga ctgtatatat
     2041 taaagccacc atttcgaata aatccaactg ccgtaatatt taggtcatta gctaaggtta
     2101 cagcaagcgt tgtcggagca gatttagata aaatgacgcc aacaccaatt ttcgcggctt
     2161 taattaaaat ttctgatgaa atacgtccac tgaaaattaa tactttatct cggacagtaa
     2221 tatgtcgctg aatacaaaat ccatataatt tatctagagc gttatgtctg ccaatgtctt
     2281 gtcgatgtac aaaaaatgtc aaaccatcgc ttagagcagc attatgcagg ccacctgttt
     2341 cttggtaaat atgactggca ctttgtaaac gcgtcatcat attaataatt tgttgtggtg
     2401 ttaaagtgat tttagacata gatgttttag caacagcagc atcattttga aaataaaact
     2461 cacgactctt tccacaacaa gatgcaatca ttcgttttgt ggaatattga aagcgatcgc
     2521 ctaaatcttt attcagttca acatgggcaa atcctttact atcatcaatc agtacagatt
     2581 ttaattcatc tcgctttaaa atggcacctt ccgaagccag aaatccaatg actaactcct
     2641 caaggtttgt tggactgcat ataacagtcg caaattcttc accattcacc ataattgtaa
     2701 gtggaaattc tgtcacatat tgatctgttg tattgaataa ttttccatct tcatatctaa
     2761 caattggttg acctaaagat acatctttgt tcattatcta acccctttaa ttagcttgaa
     2821 ctttatttta aatcaatatg cttaatattt taacatattt gcttaaaact gaaatttgat
     2881 taactaaata aaatagcagg caattattta gaatttgatt ggatttagaa aaaggcacac
     2941 tagcagctca attaataaaa aataattttt agaaagtgtg acaattattt gaatcattat
     3001 ttaaagagaa aacgcttctt ttcaaaaata ttgttaagtg tataatgaga gtgtaaatat
     3061 ttaataattg ggggtatttc ataatgaaaa tgaaacgttt tatagctatt gtaatggcat
     3121 tatttttagt attagctggt tgttctaatt ctaatgataa tcaagacaac aaaaaagatg
     3181 cagcagacaa tggaaaaaaa caagaaattc aagttgcagc ggcagcaagt ttaacagatg
     3241 ttacaaaaaa attagcatca gaatttaaaa aagaacataa aaatgctgat attaaattta
     3301 actatggtgg atcaggagca ttaagaaaac aaattgaatc aggcgcacct gttgacgtat
     3361 ttatgtctgc aaatactaaa gatgtagatg cattaaaaga caagaataaa gcacatgata
     3421 catataaata tgcgaaaaat agtctagtat taattggtga taaagattca aattacactt
     3481 cagtaaaaga cttaaaaggc aatgataaat tagcgttagg tgaagtgaaa actgtaccag
     3541 caggaaaata tgcgaaacag tatttagata acaataactt gtttaaagaa gttgaaagta
     3601 aaatcgttta tgctaaagat gtaaaacaag tattaaatta tgttgaaaaa ggtaatgcga
     3661 aacaaggttt tgtatataaa actgacttat ataaacaaaa taaaaaaatt gatactgtaa
     3721 aagtaattaa agaagtagaa cttaagaaac caatcacata tgaagctggt gcgacatcag
     3781 atagtaaatt agctaaagag tggatggaat tcttaaaatc agataaagct aaagaaatat
     3841 taaaagaata ccactttaca gcataaggag ttgtaatcca tgcctgactt aacacctttt
     3901 tggatatcaa tacgagttgc tgtaatcagt acgattattg taacgatttt aggtattttt
     3961 atatctaaat ggttgtatcg tcgtaagggt tcgtgggtta aagtattgga aagtttattg
     4021 atattaccta ttgttttgcc gccaacggta ttaggtttta ttctattaat catcttctcg
     4081 ccaagaggac caattggtca attctttgcg aatgtactac atttacctgt agtattcact
     4141 ttgacaggtg ctgtgatagc atctgtcatt gttagttttc cactaatgta tcaacatact
     4201 gtgcaaggcc tcagaggtat agacacgaaa atgattaata cagctagaac gatgggagca
     4261 agtgaaacga aaattttcct caaattaatt ttaccattag ctaaacgctc tattttagca
     4321 ggtataatga tgagttttgc tcgtgcatta ggtgagtttg gtgctacatt aatggttgca
     4381 ggatatattc caaataaaac gaatacacta cctttagaaa tatacttctt agtggaacaa
     4441 ggtagagaaa atgaagcgtg gttatgggta ttagtgctag tcgcattctc tattgtggtt
     4501 atatctacaa ttaatttatt gaataaagat aaatataagg aggtcgacta gatgcttaaa
     4561 atcaatttaa aatatcaatt aaagaatact ttaattcgca tcaatataga tgatactgaa
     4621 ccaaaaattt atgcagttcg tggtccatct ggcattggta aaactactgt tttaaatatg
     4681 attgccggat tacgtaaagc agatgaagct attatcgaag tgaatgggca attgcttact
     4741 gatacggcaa aaaacgtgaa tgttaaaatt caacaacgac gtattggata tttatttcaa
     4801 gactaccaac tgtttcctaa tatgacggtc tataaaaata ttacttttat ggctgaacca
     4861 tctgaacaca tcgatcaatt aatccaaact ttaaatattg atcatttgat gaaacaatat
     4921 cctatgacat tgtcaggtgg agaggcacaa cgtgtagcac ttgcacgtgc acttagcacg
     4981 aaaccagatt taattttatt agatgaacct ttttctagtt tggatgatac tacaaaagat
     5041 gagagtatta cattagttaa acgtattttc aacgaatggc aaataccaat catatttgtg
     5101 acacattcaa actatgaagc agaacaaatg gctcatgaaa ttattacaat tggttaatga
     5161 tacatttgcc ttgaaagagt ttagaacgta tttaaaaatg tagaagtgca atcttctatt
     5221 agcattttaa tgatgtttta aactcttttt taggcgcaat ttttgagaga cattgacgca
     5281 cgtcatataa tgaaagtaag gataaataga aaggataact taatgtgagt caagaacgtt
     5341 attcaaggca aattttattt aaacaaatag gtgaaatagg tcaaaacaaa ataaatcaaa
     5401 aatgcgcgtt gattattggt atgggcgcat tgggtacaca tgtggctgaa ggtcttgtga
     5461 gagcaggcat tgctaaacta atcattgttg acagagatta tattgaattt agtaatttac
     5521 aaagacaaac attatttact gaagaagatg ctttgaaaat gatgcctaaa gtggttgcag
     5581 ctaaaaagca tttgctagcg ttacgtagtg atgttgatat tgatgattat attgaccatg
     5641 tggattatta ttttttggag acacatggac aggacgttga cgttatcatt gatgcaaccg
     5701 ataactttga aacacgacaa ctgattaatg attttgcata taaacatcgt ataccttgga
     5761 tttatggagg tgttgtacag agtacatata cagaagctgc atttatacct ggtaaaacac
     5821 cttgctttaa ctgtttggta ccacaattgc cagcattaaa tttaacatgt gatacagtag
     5881 gggtcattca acctgccgtg acgatggcaa caagtttaca attaagagat gcgatgaaag
     5941 tattaacgga acaaccaatt gacacaaaaa taacttatgg cgatatttgg gaaggtagtc
     6001 attattcatt tggtttcagt aaaatgcaac gtacagcctg tacaacttgt ggagatgtac
     6061 caagttatcc gtatttaaac aagaatgaac aacgttatgc aacattgtgt gggagagaca
     6121 ctgtacagta tgaaaataca tcaattacac acgatattct tgttcaattt ttaaaacagc
     6181 atcaactaaa ttatcgcagt aattcatata tggttatgtt tgaatttaaa gggcaccgca
     6241 ttgttgcttt taaaggtgga aggtttttaa tacatggcat gacacgcaca tcagatgcca
     6301 cacatctaat gaatttattg tttggataaa aaggtaaaac aaaaggagtg taatattatg
     6361 ggcgaacatc aaaacgttaa attgaatcgt acagttaaag cagccgtact aacggtatca
     6421 gatactagaa actttgatac agataaaggt ggtcaatgcg tgctccaact gttacaagta
     6481 gataatgttg aagttagcga tgcacattat accattgtaa aagacgaaaa agtagctatt
     6541 acgacgcagg tgaagaagtg gttagaagaa gatgttgatg tcatcattac gactggtgga
     6601 acgggtattg cacaacgtga tgtaacaatt gaagcagtaa aaccgctttt aactaaagag
     6661 atagaaggct ttggagaatt atttagatat ttgagttatg ttgaagatgt tggcacgcgt
     6721 gcattattat ctcgcgcagt ggcaggtaca gtaaatgata aattgatatt ttcaattcca
     6781 ggatcaacag gtgcagttaa attagcatta gaaaagctca ttaaaccaga attaaatcat
     6841 ctgattcatg agcttacaaa ataatttatt gatctaattg gcgttgaaaa tctccagatt
     6901 taccgccaga cttgctttca aggtaggttt cgccaataat cataccttta tcaactgctt
     6961 tcgtcatgtc gtaaatggtt aaagccgttg ctgatgcagc ggttaaagct tccatttcaa
     7021 caccggtttt gccagttgta gagacagttg tttgaatgtt taaagtataa aggggtgcat
     7081 ttgtttcatc ccagttgaag tgaacatcta tgccagtcaa tggtaatgga tgacacatcg
     7141 gaataattgt tgatgtattt ttggcagcca taataccagc gatttgagca gtgttcaata
     7201 cattgccttt ggtattggtg ttattagaaa tctgcttata aatagtttca tttacagtaa
     7261 tgcttgaatg agcgacagca gttctttttg taatttgttt gtctgataca tcgaccattt
     7321 tggcgtggcc ttgttgatta atatgagtaa actcagtcat tttacccctc ctagtgcatc
     7381 tagtatatca tgaaaaaata aaagttttgg agatgatttt taatggtagt agaaaaaaga
     7441 aacccaatcc cagttaaaga agcaattcaa cgtatcgtta atcagcagag tacaatgccg
     7501 gcaattacgg tagcacttga aaaaagtcta aatcatattt tagcagaaga tattgtagct
     7561 acttatgata taccaagatt tgataaatca ccttatgatg gttttgcaat tcgcagtgtt
     7621 gattcacaag gggcaagtgg tcagaatcga attgagttta aagtgattga tcatattggt
     7681 gcaggttcag tttctgataa attagttggg gatcacgaag cggtgcgtat tatgactgga
     7741 gcacaaatac ctaatggcgc agatgctgtt gttatgtttg aacaaacgat tgaactagaa
     7801 gatacattta caattcgtaa accattttca aaaaatgaaa atatatcttt aaaaggtgaa
     7861 gaaacaacga caggcgatgt tgttctaaaa aaaggacaag taattaatcc aggggctatt
     7921 gcggtccttg caacatatgg ctatgcagag gttaaagtta ttaagcaacc gagcgtcgct
     7981 gttattgcaa caggaagcga attattagat gttaatgatg tattagaaga tggaaaaatt
     8041 cgtaactcta atggtccaat gattcgtgcc ttagcagaaa aattaggtct tgaagttggt
     8101 atttacaaaa cacaacaaga tgatttagat agtggtatac aagtcgttaa agaagctatg
     8161 gaaaaacacg atatcgttat tacaacgggc ggagtttctg ttggagactt tgactattta
     8221 cctgagattt ataaggctgt aaaggcggaa gtgttattta ataaagtagc aatgcgtcct
     8281 ggtagcgtta caacggttgc atttgcagat ggaaagtatt tgtttggatt atctggaaat
     8341 ccatcagctt gttttacagg atttgaacta tttgtgaaac cagctgttaa acatatgtgt
     8401 ggcgcactag aagtcttccc gcaaataatt aaagcaacat taatggaaga ttttaccaag
     8461 gcaaacccat tcacacgatt tatacgtgct aaagcaacgt taacaagtgc tggagctact
     8521 gtagtacctt caggattcaa taaatcaggt gcagttgtag cgattgcaca tgctaactgt
     8581 atggtcatgt taccaggagg gtcacgtggt tttaaagcgg gtcatacagt agatattata
     8641 ttgactgaat ctgacgctgc tgaagaggaa cttcttttat gattttacaa attgtaggtt
     8701 acaaaaagtc tggtaagaca acattgatga ggcatattgt ctctttctta aagtcacatg
     8761 gttatacagt tgctactatt aaacatcatg ggcatggtaa ggaagatatt caattacagg
     8821 attcagacgt cgatcacatg aagcatttta aagcgggggc agatcaaagt attgtacaag
     8881 gatttcaata tcagcaaact gtaacacgtg tagataatca aaatcttact caaattattg
     8941 agaaatctgt tacaattgac accaatatcg tattagttga aggctttaaa aatgctgatt
     9001 ttgaaaaagt cgtagtctat cgaaatgaag aagagttgca agtattacaa caattgacga
     9061 atgtttgtta tagcattaat gtaagggagc atgaagattt tacagcattt gagcaatggt
     9121 tattaaataa aattaaaaat gattgtgata cacaattaac atagaggatt gaaatgaatg
     9181 aaacaatttg aaatcgtgac agaaccgata caaacagaac aatatcgtga attcactata
     9241 aatgaatatc aaggtgcagt agttgttttt accggtcatg ttcgcgaatg gactaaaggc
     9301 gtcaaaacgg aatatttaga atatgaagcg tatattccaa tggctgaaaa gaaattggca
     9361 caaattggag atgaaataaa tgaaaaatgg cctggaacga taacgagtat tgttcataga
     9421 atagggccat tacaaatttc agatatcgct gtattaattg cagtttcttc accgcatcgt
     9481 aaagatgcct atcgagcaaa tgaatatgca attgagcgta tcaaagaaat tgttccgatt
     9541 tggaaaaaag aaatttggga agatggttca aaatggcaag gacatcaaaa agggaattat
     9601 gaagaagcga agagggagga ataagagaga tgaaggtact ttacttcgca gaaattaaag
     9661 atatattaca aaaagcacag gaagatattg tgcttgaaca agcattgact gtacaacaat
     9721 ttgaaaattt attgtttgaa cgttatccgc aaatcaataa taaaaagttt caagttgctg
     9781 taaatgagga atttgtacaa aaatccgatt tcattcaacc taatgatacc gttgcattaa
     9841 ttccaccggt tagtggaggt taagcgagca tgaaagcaat aattcttgca ggtggtcatt
     9901 cagtaagatt tggtaagccc aaagcttttg cggaagtgaa cggtgagacc ttttatagta
     9961 gagtaattaa gacattagaa tcaacaaata tgttcaatga aattattatt agtacaaatg
    10021 cgcaattggc aacgcaattt aaatatccaa atgttgttat agatgatgag aatcataatg
    10081 ataaaggtcc attagcagga atttatacaa tcatgaagca acatcctgaa gaagaattgt
    10141 tttttgtcgt ttctgttgat acaccaatga ttactggtaa agctgtaagc acgttgtatc
    10201 agtttttagt ttctcatctt attgaaaatc atttagatgt cgcagctttt aaagaagatg
    10261 gacgttttat tccaacaatt gcattttata gtccgaatgc attaggcgct ataactaaag
    10321 cactacattc tgataattac agttttaaaa atgtatatca tgaattatca actgattatt
    10381 tggatgtcag ggatgtagat gcgccctcat attggtacaa aaatataaat tatcagcatg
    10441 atttggacgc tttaattcaa aaattgtaag ccgttaggag gtccacaaat ggtagaacaa
    10501 ataaaagata aactaggacg tcccatccgt gacttacggt tatctgtgac agatcggtgt
    10561 aactttaggt gtgattattg catgcctaaa gaggtatttg gagatgattt cgtattttta
    10621 cctaaaaatg aactgttaac gtttgatgaa atggctagaa tcgctaaggt atatgcagaa
    10681 ttaggtgtaa aaaaaatacg cattacaggt ggagaaccat tgatgcgacg ggatttagat
    10741 gtacttattg ctaaattaaa tcaaatcgat ggtattgaag atattggttt gactacaaat
    10801 ggtttgttat taaaaaagca tggacaaaag ttatatgatg ctgggctacg cagaattaat
    10861 gtcagtttgg atgctattga tgatacgcta tttcaatcaa tcaataatcg taatattaaa
    10921 gcgactacga ttttagaaca aattgattac gcgacgtcta ttggtttgaa tgtaaaagta
    10981 aatgttgtta tacaaaaagg tattaacgat gatcaaatta taccaatgct tgaatatttt
    11041 aaagataaac atatagagat tcgatttata gaatttatgg atgttggtaa tgataatgga
    11101 tgggatttca gcaaagttgt aactaaagat gaaatgctta ctatgataga gcagcacttt
    11161 gaaattgatc ctgtagaacc aaaatatttt ggggaagtag caaaatatta tcgacataag
    11221 gataatggtg ttcaatttgg tttgattaca agtgtttcac aatcattttg ttctacatgt
    11281 acacgggcaa ggctgtcatc agatgggaag ttttacggat gtttatttgc aactgccgat
    11341 ggatttaacg ttaaagcgtt tattcgttct ggcgtgaccg acgaagaatt aaaagaacaa
    11401 tttaaagctt tatggcaaat aagagatgat cgatattcag atgagagaac tgctcaaaca
    11461 gttgccaatc gtcaacgtaa aaagataaac atgaattata ttggtggtta atgtgtagga
    11521 accactacat attaaatcat tagagattct ttaatatttc tgtcttactc cctaaaatac
    11581 aatattattt attaaagtaa aaacggtcat atctatgcca aatttaatag aaatgatcgt
    11641 tttaaatatt tttacaaatt ggcggggccc caacatagaa ggtgacgaaa agtcagctta
    11701 caataatgtg caagttggcg gggccccaac atagagaatt tcaaaaagaa attctacaga
    11761 caatgcaagt tggggaacaa cgaaataaat tttatgaaaa tatcatttct atcccactct
    11821 taagaatcac tacataataa atctttagtg gctctttaac attgttgtca cactccaagt
    11881 cattgagttg taatatatct tttttaggta taaatgttgt cgaataaaca acaagttgtc
    11941 caaaagatat aaatctaaac aagatatagc cagcaattta atatttgtaa tagataaaat
    12001 gctaagtttg atatataata aatttaagta attgtataat aatatgaatt acaaacatct
    12061 aagaagaaac ataggaggca tcatattatg agtaataaag ttcaacgttt tatagaagca
    12121 gaaagggagt taagtcagtt aaagcactgg ttaaaaacaa cacataagat ttcaattgaa
    12181 gaatttgtag tactttttaa agtgtatgaa gctgaaaaga ttagcggtaa agaattgagg
    12241 gatacattac attttgaaat gctatgggat acaagtaaaa tcgatgtgat tatccgtaaa
    12301 atctataaaa aagagcttat ttctaaattg cgttctgaaa cggatgaaag acaagtattc
    12361 tatttctata gtacttctca aaagaaattg ttagataaaa ttactaaaga aatagaagtg
    12421 ttaagcgtta caaactaaaa acttaaaaag catgccaatc agtacatcat aattgcgtct
    12481 tggggacaga caaatgatga atagagattg gcatgctttt tatttttgaa tataaatatt
    12541 tagttcatgg catttctagt tacatgacgt ccatgaatta agaagtaaac aagtatagta
    12601 atgattgcta aagcggccat aaaaccgaag atttcactat atgaaaacat atgagtaaat
    12661 aacccaagga atgatggacc gaagccgaca cctgcatcta gaccaacgta aaaagtagat
    12721 gtcgcgatac catatttaat cgggggtgag acttttatcg caatagattg cattgcagat
    12781 gataaatttc cataccctaa acctaggcaa gcaccagcaa gtaatattaa ccagctttga
    12841 tagcttgaaa ttaagcacac aaatgaaagg aaaagcatga taaatgctgg gtagacaata
    12901 atattttcat ttttatcatc catcaatcta ccagcaatag gtctagtaat taacgatgct
    12961 atagcatagc aaataaagaa atagcttgct gcagttacta ggtgtcgctc tatagcaaat
    13021 gcttgtaaat aagttaggat ggacgcatag gtaacgccaa ttaaaagcat aataacagca
    13081 acaggaatgg cctcttttgc aataaattga tgaatactaa atcttggttt atcaatgaca
    13141 ttagtttcag ttttgctatt tgttacttcg aaatcaactt ttataaataa tgagataatg
    13201 actccgagta tgcctaatat aacacaaata ataaacagta aatcaattgc gtattttgta
    13261 ataagtaaca tgcctagaaa tgggccaatc gctgtaccta atactaaact taaagaaaat
    13321 aaactgatgc cttcactttt tctattaaca ggggtaacgt atgccgcaat agtacctgtt
    13381 gcagttgtca caactgcagt tgcgataccg tttatgagac gtacaaagat taaaaaagct
    13441 aaagatccat caataaaata aagtaattgc gtgataatta aagcaattaa accaataaat
    13501 aataatcgtt taggtccgat ttgatttaca aatttacctg tagcaaatcg accaattaaa
    13561 gaaccgacaa taaatagtcc tacaactaat ccggctaatg aatcggaagc gtcgaaagct
    13621 tgcttactat agccagcaat aactacaagt aacaaataca tgcataggta tacgataaaa
    13681 ttgacaaaaa agttaattgt aaaacttttg gtgaaaatag gtgatttatt taatgagcct
    13741 tgattagtca ttgtgtgcct cctttaataa tatctcatga atttgattca tcacatttat
    13801 ggtttcttgc atttgatctt gtgaaatgtt tgttttggca actactgctt gttggagctt
    13861 ttgtacatca agacaaacaa tctcatataa ttcatgccct ttaggtgtta aagttaaaaa
    13921 cttttgtcgt ttatcctcta aactgttttc tactgttata aggtcatttt ctattagagc
    13981 ttttaaagtt tttcttgcag taggcttttc aattgcacgt ctatgtgaaa tttcaatgag
    14041 agtagtgggt tgatgtttag cgatatcgcg taagattaac cattgaccat aatatatatt
    14101 gtgtttttcc aaaataggct ctgttaattt taaatacggg cgatatattg ttataaaact
    14161 attgaaaaat tcttgtgata gcatagcgac atctcctaag acaaaatagt tagcttagct
    14221 aacctttttt acaacaatag taattataaa acggggacaa ttagaaatca atatataatt
    14281 attaagagca aaaatattta tactttgtta aaataagcgt aattacatgt aaataggggg
    14341 atactaatga tattgaaatt tgatcacatc attcattata tagatcagtt agatcggttt
    14401 agttttccag gagatgttat aaaattacat tcaggtgggt atcatcataa atatggaaca
    14461 ttcaataaat taggttatat caatgaaaat tatattgagc tactagatgt agaaaataat
    14521 gaaaagttga aaaagatggc aaaaacgata gaaggtggag tcgcttttgc tactcaaatt
    14581 gttcaagaga agtatgagca aggctttaaa aatatttgtt tgcgtacaaa tgatatagag
    14641 gcagttaaaa ataaactaca aagtgagcag gttgaagtag taggaccgat tcaaatggaa
    14701 agagatacac ataaagatgg taaggtaaag tggcaattgc tttatataat gaatcaggat
    14761 gatgatgaaa ttaagccacc attttttatt caatgggaag aaagtgattc catgcgtact
    14821 aaaaaactac aaaaatattt tcaaaaacaa ttttcaattg aaactgttat tgtgaaaagt
    14881 aaaaaccgat cacaaacagt atcgaattgg ttgaaatggt ttgatatgga cattgtagaa
    14941 gagaacgacc attacacaga tttgatttta aaaaatgatg atatttattt tagaattgaa
    15001 gatggtaaag tttcaaaata tcattcggtt ataataaaag acgcacaagc aacttcacca
    15061 tattcaattt ttatcagagg tgctatttat cgctttgaac cattagtata aatatacgta
    15121 agtgctatga gcgagaatgc ccatatgaat aatggcaagc gcaatggaaa gaatcgttaa
    15181 tatattattt aatcgtgatg acttaattaa aatgaaaaag attgataata taaatgtgaa
    15241 aaagataagt ataacccgta aactaaagta attcacggtg agaggttgac tcaatgtcat
    15301 aatgattgca acgatgttca taattataaa tagacttaaa ataattgttc tcatatcaaa
    15361 cacctcattg ttagattatt gacattataa caggggtaat tgtatatgaa cattaatgtg
    15421 gttgcttgag gagaaattta ttcattgaag tcaaattggt tcatgttaga aatgaatatc
    15481 gtgttagatg atgaaagtat attgaagtat aggtaactag ttgaaaagta ttaattgtac
    15541 gataacatta aatttaacag gaagcataga tataaaatga ttcacaatta aaatgagtaa
    15601 atttgaactt gctaaactat taattggagc atggacattt caaaaataag agttcaaatc
    15661 ttacacaagc tctgaatcga cactataaga tacaaactgt ataattaaag gtattgttaa
    15721 atagaaggag atatcataaa tcatggaaaa gatgcatatc actaatcagg aacatgacgc
    15781 atttgttaaa tctaacccaa atggagattt attacaatta acgaaatggg cagaaacaaa
    15841 aaaattaact ggatggtacg cgcgaagaat cgctgtaggt cgtgacggtg aaattcaggg
    15901 tgttgcgcag ttacttttta aaaaagtacc taaattacct tatacgctat gttatatttc
    15961 acgtggtttt gttgttgatt atagtaataa agaggcgtta aatgcattgt tagacagtgc
    16021 aaaagaaatt gctaaagctg agaaagcgta tgcaattaaa atcgatcctg atgttgaagt
    16081 tgataaaggt acagatgctt tgcaaaattt gaaagcgctt ggttttaaac ataaaggatt
    16141 taaagaaggt ttatcaaaag actacatcca accacgtatg actatgatta caccaattga
    16201 taaaaatgat gatgagttat taaatagctt cgaacgccga aatcgttcaa aagtgcgctt
    16261 ggctttaaaa cgaggtacga cagtagaacg atctgataga gaaggtttaa aaacatttgc
    16321 tgaattaatg aaaatcactg gggaacgcga tggcttctta acgcgtgata ttagttactt
    16381 tgaaaatatt tatgatgcgt tgcatgaaga tggagatgct gaactatttt tagtaaagtt
    16441 ggatccaaaa gaaaatatag cgaaagtaaa tcaagaattg aatgaacttc atgccgaaat
    16501 tgctaaatgg cagcagaaga tggaaacatc tgaaaagcaa gctaaaaaag cgcaaaatat
    16561 gattaatgat gcgcaaaata aaattgctaa aaatgaagat ttaaaacgag acctagaagc
    16621 tttagaaaag gaacatcctg aaggtattta tctttctggt gcactattaa tgtttgctgg
    16681 ctcaaaatca tattacttat atggtgcgtc ttctaatgaa tttagagatt ttttaccaaa
    16741 tcatcatatg cagtatacga tgatgaagta tgcacgtgaa catggtgcaa caacttacga
    16801 tttcggtggt acagataatg atccagataa agactcagaa cattatggat tatgggcatt
    16861 taaaaaagtg tggggaacat acttaagtga aaagattggt gaatttgatt atgtattgaa
    16921 tcagccattg taccaattaa ttgagcaagt taaaccgcgt ttaacaaaag ctaaaattaa
    16981 aatatctcgt aaattaaaac gaaaatagat taacgactga aatctgaacg ctcataagac
    17041 tgtcatttgc gttcagattt ttttacacaa tatagaatgg ttgagtaaaa tatttttgaa
    17101 tatagtgaaa gagggggaag tactgtgata aaaaagctat tacaattttc tttggggaat
    17161 aagtttgcta tctttttaat ggttgtttta gttgtcttgg gcggtgtata cgcgagtgct
    17221 aaattgaaat tagaattact accaaatgta caaaatccag ttatttcagt tacaacatca
    17281 atgccgggtg caacgccaca aagtacccaa gatgaaataa gtagtaaaat tgacaatcaa
    17341 gtaagatcgt tggcatatgt gaaaaatgtt aaaacgcaat ccatacaaaa tgcttcaatt
    17401 gtaacagttg aatatgaaaa taatacagat atggataaag cagaagaaca gcttaaaaaa
    17461 gaaatcgata aaattaaatt taaagatgaa gttggtcaac cagaattaag acgtaattcg
    17521 atggacgctt ttccggtttt agcatattca ttttcaaata aagagaatga cttgaaaaaa
    17581 gtaacgaaag tactgaatga acaattaata ccaaaattac aaacggtaga tggtgtgcaa
    17641 aatgcgcaat taaatgggca gacgaaccgt gaaatcaccc ttaaatttaa gcaaaatgaa
    17701 cttgaaaaat atgggttgac tgctgatgat gtagaaaact atctaaaaac ggcaacaaga
    17761 acaacgccac ttggattgtt ccaatttggt gataaagata aatcaattgt tgttgatggt
    17821 caatatcaat ctgttgatgc ttttaaaaac ataaatattc cattaacgtt ggcaggaggg
    17881 caagggcaat ctcaatccca aagtgacaat aaacaaaatt cagccatgtc agacgttaat
    17941 caggcatcac cgcagcaaaa ttcaaaaacg tcagcatcaa ataatataag tggtatgccg
    18001 acagcgaaac taggagattt agctgatatt acagttggtg atgtgcgtac ttctatttct
    18061 aaaacgaatg gcaaggatgc ggttaatcta caaataacta aagctcaaga tgccaataca
    18121 gttcaagtag ccaaagatgt acagcgtaaa attgatacat tcgttgatga aaataaagat
    18181 tttaatgtca caaaaacaat ggatactgca aagcctgttg agaaatcact ttatacgatg
    18241 gttgaaaaag catcattagg tacaatcgtg gcaattatag ttattttgct gtttttaaga
    18301 aacattcgta cgacggcaat ttctattata tccattccat tatcacttct tatggcgctt
    18361 attgctctga aattgagtga tgtttcattg aatatactaa cgttaggtgc attaacagta
    18421 gcgattggac gtgtgataga cgattcgatt gtagttgttg aaaatattta tcgacgctta
    18481 acagattcag aagaacaact aaaaggtgaa aatttaatta tcagtgcgac aactgaagta
    18541 tttaaaccaa taatgtcatc gacactagtt actattatcg tcttcttacc acttgtgttt
    18601 gtatcaggtt cagtaggaga aatgtttaga ccttttgcat tggctattgc atttagttta
    18661 ctagcatcgt tattagtgtc aattacactc gttccagcgt tggcagcaac actatttaaa
    18721 aaaggcgtta aacgtcgtaa taaacaacat caagaaggat taggtgttgt tagtacaact
    18781 tataaaaaag tattgcattg gtcactaaat cataagtgga ttgtaattat attaagtaca
    18841 ttaattttgg ttacaactat tgtatttgga ggaccgagac taggcactag ctttatttca
    18901 gcaggtgacg ataaattttt agctattact tatacaccga agcctggtga aacggagcaa
    18961 gcagtgttga atcatgcgaa agatgttgaa aaatatttaa aacagaaaaa gcatgtaaaa
    19021 acaattcaat actcagttgg cggtagtagt ccagtagatc caacgggtag tacaaatagt
    19081 atggcaatca tggttgaata tgataatgac acgcctaatt ttgatgtaga agcggataag
    19141 gttattaaac atgcagatgg ctttaaacat cctggagagt ggaaaaatca agatttagga
    19201 acaggtgcag gtaataaatc tgtagaggtt actgtgaaag gtccatctat ggatgccata
    19261 aaatcaactg taaaagatat tgaacagaaa atgaaacagg ttaaaggtct agccaatgtc
    19321 aaatctgatt tatcacaaac atatgatcag tatgaaatta aaattgatca aaataaagcg
    19381 gcagaaaatg gtatttctgc aagtcaactt gcaatgcact tgaatgaaaa cttaccagaa
    19441 aaaacagtta cgactgttaa agaaaatggt aaaactgttg atgttaaagt caaacaaaat
    19501 aagcaaacag actggtcaga agataagttg aataatataa ctttgaaaaa gccgactggt
    19561 ggtacgatta aattgggaga tatcgctacg ttagttaaaa caacgacacc aagtaaattg
    19621 acgcaagaac aaggtgatta tgcaacgacg gtatctgcta aagtaacaaa taaagatgtg
    19681 ggtggcacaa cacgacaagt gatgtctaaa ataaataatt tggacaaacc gaataatgta
    19741 aaggttaata tcggtggtgc atcagatgat attaacaatg caatgacaca attagccttt
    19801 gcaatgttag ctgcaatcat tatcgtatat ttaatcctag ttattacatt taaaggtggc
    19861 ttagcaccat ttacaatttt attctcttta ccatttacag ttatcggtgt aattattgca
    19921 ctattaatca caggagaaac aatatcagta ccaagtttaa ttggtatgct aatgttaatt
    19981 ggaattgtag taacaaatgc cattgtgtta atagaccgtg tcattaataa tgagcagcag
    20041 ggcatggaga tgaaagaagc attaatcgaa gcaggcggta ctagaattag accgatatta
    20101 atgacggcga ttgcaacaat cggtgcatta gttcctttgt tatttggtca agatagctcg
    20161 attcttattt cgaaaggatt agccgctaca gttattggtg gtttaatttc atcaacttta
    20221 ttaacacttg tagttgtacc agtaatatat gaaattctct ttactttaaa aaaacgattc
    20281 actaaacgat agataatata aaagccataa aagcggtcat gatgttgact acttttatgg
    20341 ctttttaaaa tttgaaattt tagaccttat ttcacgtgcg atttaatcga tgtgttaata
    20401 aaacaatact ggcattcgat atgtaatctg atgataatat aaggttgcaa ttatgttatt
    20461 gcatgttttg tttattactt agaatagtac gatggttaag atgaaatata tgacgaagat
    20521 aaataatgac attaaattca agatgtttac gccttttaga tcttttttaa gcattttaaa
    20581 tcctgtcact tcgacattac caactaagag taataagacg ataaataccc accaattctc
    20641 ttttggaaaa ctcatcgtcg ttaatgctaa gacgaataat aacaacacac ccataataat
    20701 tctcaaaatt cgagtaaata caacttccat tttaaaaata ctaataaaac taacaattag
    20761 ataaagtatt gctagtaaaa tcagataaaa ttgcatatga taacttcctc tccgtttcaa
    20821 agggttcatg tagtacagta gcgtagaaaa cataaatagc agtgttggga tagatatgcg
    20881 cgtatgttgt taatgagaca ctaaatcata acacgacaac atcttgtgat tctagatgta
    20941 attgagcgaa aaatgaagca attcatcaat ataacgtatt cagagcaatg ctagtcaaat
    21001 ttatgtaatg aaaagcattt attgtctgaa tgatagatta ttaatcttgc atttgagcac
    21061 gaacgtttct aatttcattt tcaatatctt taagttgacg aacaagagcg gaagtatcta
    21121 aattcaaaga ttcccgtttt tctaagtcac cttgaagacg aacataatca tatttaagtt
    21181 cggctagttg gttttctaaa gacatactat aacactcctt tgaaatataa ttaagtgcat
    21241 ttaatgtaga ttatagcatg aaacagtaga cttctaaaat atctgtggag tatgttaaag
    21301 ttataaatta tgaagattat tgaaagaagg gataacagtg acagaacaat ttactatgat
    21361 tattttatta attgcgcttg gttacttttt gaagcgcatt aattatataa aggcaacaga
    21421 tagtcaagtc attgctacat tagttcttaa tgtcacactc ccatctttag ttattgtgaa
    21481 tttaaacagt gcagaattaa aattgtcatt atccatttta ccaattttaa tgattatcta
    21541 tggtattgtg gcgaaaatga ttgctgtctg gttttttaga aaatatgata atcacatgcg
    21601 tggatcagta ggtatgatga ctggtgctat gaatattgga ttgtttgcat atccactaat
    21661 tgaagtgata tggcctaaaa cgggacttat ttattttggt atggctgata taggtggtgc
    21721 ctttgtgatg tttggaatta cttactttgt aggtagctac tttagtgaag gtagtgatca
    21781 attcgatttt aaatatttag gtaagaaatt attacaatca gtgccactcg ttacatacat
    21841 tgtaatgttt atattgaata taagtcacat ccatttacca catgtggcaa tcgatttctt
    21901 tagcatactt tcccatgcaa atatgccgtt atcaatgatt ttgctaggtg tcatgttgaa
    21961 ttttactctg gaaagaaagt atatacctgc tactattaaa tatttatgtt tacattatgg
    22021 attagcatta gttgcgggat tgttagtaca tgtgttgcta ccggtatcag atgatatgat
    22081 taagacgaca ttgttggtca cttggatgtt cccggttggt gtcgcaatta tttcttatgg
    22141 tattcagttt aaatatcgaa cactgccatt tataggtatg acgaccaatt taacaatcat
    22201 tattagtatc attattttgt acgtgtatca ggcagtgttt gtctagttta cacatttgaa
    22261 aataaaatcc attttagtat tcgtagaaaa tgagagcccc ctttaccaca atatgtaaat
    22321 gcatagtgtg aaaagggggc ttttatttat gacatagttg ttcaactcat acattctagc
    22381 agataatgca gtgcgttatg cagctatcca cttcatatgt atggcgcata cctgataaac
    22441 gaatatcatc aagactttaa aagtatcgct aattttaaag tcttaaaccg catgattaat
    22501 gtcatgatgt gttattgcgt tttgttttta gagccatgta tttcctggga catatagagg
    22561 aacattcaac gtaaaattca cgaaaattaa ttttaaacat tctaaaagtg aatggttatt
    22621 ttcagaaacg ataacatttt tacgttacat gagtatttaa ctttctattt caaattacct
    22681 agaattatag cagctatcac gataattata atacctaccc aaataccagt catctgacga
    22741 cgatcttttc tttcgcctaa aatgaaaata ccgcctaatg ttgaaacgat aacaagtaac
    22801 tgtgataatg agaaacttgt agctacacca acttttggtt gagaatagaa catgaacaag
    22861 ttaccaattc cccaaatcac acctggcaat agattaagtg ctgttgattt aagtgatgtt
    22921 ttatgattca tggataggat aaagccacca attgccatac cgacagattg gaagaacaat
    22981 gcatcagttc caccaacacc aaagatgtca ccaagtacaa cgaaacctac atatccaact
    23041 gtagatacaa ttaaaatacc cattgctttt ttaaattcag gattatctga ttgacgttca
    23101 tttttagctt taagtgaagt aagtgctaca ccagtaacta ataatatcat ggcgattaaa
    23161 ccaaagataa tttgagtcat tgaagaccat tcacctaaaa agataacact gaataatgtt
    23221 gtaccaacta attgcatacc tgtagaaact ggcatagtat ttgatacacc gattaaacta
    23281 atagatttaa gctgattcgc ttgtccaaac gcccataatg caccagaaat aagaccgaca
    23341 ataattacag taggattatt gaattttgcg aaaccagtaa taagtaaacc taatccaaca
    23401 atgagtgcgc ctagcgtcgt accacgaatt tggttgtaag gtccaccgcc gacgaacaca
    23461 ttaataagaa cgacacttcc ccagaataaa gcaggtaaaa gtgcgattaa gaaatcaaga
    23521 aattgcaaga ctactcactc tcctatacta tttatgcaag tgctcccctt aattgacact
    23581 tgcatgttat tgtaaagcat tctgccctaa tagtaaaaaa caactactta taattttcaa
    23641 tatttacaaa aagtagaaaa acccacaata catgctaatg atgtactgtg gggtatttgc
    23701 ttggtatttt attcagggat tgtttgatct tttacgttac gtgattcgaa tttgaattta
    23761 atgtactcat agttgatatg gaaacctaga ccattaaatc gttcgatgag tttattattt
    23821 ttgttacgaa ttttaaaata aacttttcta atatcaaagt tatcaaaagc atattgcaat
    23881 gcatacgcaa gcaactcgaa agcgatacct ttcaaacggt aatctacatg agaactgaag
    23941 tatttaatct cggcgattga ctgttgtgaa tcaatttcaa ggtataaata acctttaagt
    24001 aaaccttcgc taacaaataa aaacaaatga tgatggtcgt ctaaactatc aatgatatct
    24061 tgtggtgact gggcctgata tttaaatgta gttgtgtgta atttgctgaa agcacgataa
    24121 aaccctttgt gataaggaat gatatttggt tggtgcatat cttcttctaa tcttgtacgg
    24181 gcttctatgt aatagtcagt gaagttataa cttgcttgaa taactttcat taatggtttg
    24241 tattgttgaa tgccttcttc aaaagaaaag ttaaagacgg catcgtcagg ttgattcgaa
    24301 gtcattgttt taaacagcg
//
LOCUS       DAMZPP010000022        24013 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMZPP010000022 DAMZPP010000000
VERSION     DAMZPP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24013
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.022"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            14..1351
                     /locus_tag="POH73_000689"
     CDS             14..1351
                     /locus_tag="POH73_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDI6886664.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILKSMKQVEKHSDYDGYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLVVLTYPNYYGET
                     FNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRENIIEYLSYFQTSSPSYLIMASLESAAQFYKTYDSTLFFAKRAQLI
                     ECLENKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            1353..1970
                     /locus_tag="POH73_000690"
     CDS             1353..1970
                     /locus_tag="POH73_000690"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDI6886665.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            1998..2327
                     /locus_tag="POH73_000691"
     CDS             1998..2327
                     /locus_tag="POH73_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDI6886666.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            2541..3467
                     /locus_tag="POH73_000692"
     CDS             2541..3467
                     /locus_tag="POH73_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDI6886667.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQDMSKPVAEMISTYTTQIDNALALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDSDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            3468..4271
                     /locus_tag="POH73_000693"
     CDS             3468..4271
                     /locus_tag="POH73_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDI6886668.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVELDDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            4288..4635
                     /gene="yabA"
                     /locus_tag="POH73_000694"
     CDS             4288..4635
                     /gene="yabA"
                     /locus_tag="POH73_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDI6886669.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            4909..5634
                     /locus_tag="POH73_000695"
     CDS             4909..5634
                     /locus_tag="POH73_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDI6886670.1"
                     /translation="MLKENERFDQLIKENFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRADRLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            5627..5875
                     /locus_tag="POH73_000696"
     CDS             5627..5875
                     /locus_tag="POH73_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDI6886671.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            5877..6716
                     /gene="rsmI"
                     /locus_tag="POH73_000697"
     CDS             5877..6716
                     /gene="rsmI"
                     /locus_tag="POH73_000697"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDI6886672.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIN"
     gene            7001..8974
                     /gene="metG"
                     /locus_tag="POH73_000698"
     CDS             7001..8974
                     /gene="metG"
                     /locus_tag="POH73_000698"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDI6886673.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMINKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLTEP
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9005..9778
                     /locus_tag="POH73_000699"
     CDS             9005..9778
                     /locus_tag="POH73_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDI6886674.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            9945..10481
                     /gene="rnmV"
                     /locus_tag="POH73_000700"
     CDS             9945..10481
                     /gene="rnmV"
                     /locus_tag="POH73_000700"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDI6886675.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHADLIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            10492..11385
                     /gene="rsmA"
                     /locus_tag="POH73_000701"
     CDS             10492..11385
                     /gene="rsmA"
                     /locus_tag="POH73_000701"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDI6886676.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEIGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEVFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVIIQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            11485..11748
                     /locus_tag="POH73_000702"
     CDS             11485..11748
                     /locus_tag="POH73_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDI6886677.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            12059..12907
                     /locus_tag="POH73_000703"
     CDS             12059..12907
                     /locus_tag="POH73_000703"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDI6886678.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFNIGASLEELALLGSKIGTDIPFCIYNKTALCTGKGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLAQKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            12921..13745
                     /gene="purR"
                     /locus_tag="POH73_000704"
     CDS             12921..13745
                     /gene="purR"
                     /locus_tag="POH73_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDI6886679.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            13762..14142
                     /locus_tag="POH73_000705"
     CDS             13762..14142
                     /locus_tag="POH73_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDI6886680.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNVDGKIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            14213..14515
                     /gene="spoVG"
                     /locus_tag="POH73_000706"
     CDS             14213..14515
                     /gene="spoVG"
                     /locus_tag="POH73_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDI6886681.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDEIDEVVPDKNATSEDSEEA"
     gene            14704..14835
                     /locus_tag="POH73_000707"
     CDS             14704..14835
                     /locus_tag="POH73_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886682.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            14859..16211
                     /gene="glmU"
                     /locus_tag="POH73_000708"
     CDS             14859..16211
                     /gene="glmU"
                     /locus_tag="POH73_000708"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDI6886683.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAENAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            16358..17323
                     /locus_tag="POH73_000709"
     CDS             16358..17323
                     /locus_tag="POH73_000709"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDI6886684.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            17473..18126
                     /locus_tag="POH73_000710"
     CDS             17473..18126
                     /locus_tag="POH73_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDI6886685.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            18437..19009
                     /locus_tag="POH73_000711"
     CDS             18437..19009
                     /locus_tag="POH73_000711"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDI6886686.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            19009..22515
                     /gene="mfd"
                     /locus_tag="POH73_000712"
     CDS             19009..22515
                     /gene="mfd"
                     /locus_tag="POH73_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDI6886687.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFVDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNIEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIDYFQKDAIIAVDEFNRIKETEESLTVESDSFISNVIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQIHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQS
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            22505..>24013
                     /locus_tag="POH73_000713"
     CDS             22505..>24013
                     /locus_tag="POH73_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDI6886688.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGMLLFIAIFVFANN
                     IAHMMGDSHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLHAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRITGLVELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQI"
BASE COUNT         8636 a         3250 c         4798 g         7329 t
ORIGIN      
        1 ggaagcgtat atcatgaagc aacctatttt aaataaatta gaaagtttaa atcaagaaga
       61 agcgatttct ttgcatgttc cgggtcataa aaatatgact atcggtcatt tatctcaatt
      121 atcaatgaca atggataaaa ctgaaatacc tggattagat gatttacatc atcctgaaga
      181 agtcattttg aaaagtatga agcaggtgga gaaacattca gattatgatg gttatttctt
      241 agtgaatggc accacttcgg gaatattatc tgtcatccag tctttttcac agaaaaaagg
      301 cgatatctta atggcaagaa atgtacataa atctgtatta catgcgctcg atattagcca
      361 acaagaaggg cattttattg aaacgcatca aagtccgtta acgaatcatt ataataaagt
      421 taatttaagc cgtttgaata atgacggtca caaacttgtt gtgttgactt atcctaacta
      481 ttacggtgaa acatttaatg tagaagaggt tatcaaatct ttgcaccaat taaatattcc
      541 tgtactcatt gacgaagcac acggcgcgca ctttggattg caaggatttc cagattctac
      601 attaaattat caagctgact atgttgttca atcttttcat aaaacgttac cagctttaac
      661 gatgggctcg gtactttata ttcataaaaa tgcaccttat agagaaaata ttatagaata
      721 tctaagctac ttccaaacat ctagtccttc atatttgatt atggctagtt tagagtcagc
      781 tgcccagttc tataaaacat atgatagtac cttgtttttt gctaagagag cgcaattaat
      841 cgaatgtttg gagaataaag gttttgaaat gcttcaagtt gatgatccgt tgaagctgtt
      901 gataaaatat gaaggcttta caggtcatga tattcaaaat tggtttatga atgcacatat
      961 ctatttagaa ttagcggacg actatcaagc attagcgata ttgccgttat ggcatcatga
     1021 tgatacgtat ttatttgatt cgcttttacg taaaattgaa gatatgattt taccgaaaaa
     1081 atcagtttct aaagttaaac aaacacaact tttaacaact gaaggtaact ataaaccaaa
     1141 acgctttgaa tatgttactt ggtgtgattt gaaaaaggca aaaggtaaag ttttggcgcg
     1201 acatattgtc ccgtatccgc cagggattcc tattattttc aaaggagaaa caataactga
     1261 aaatatgata gaattggtaa atgaatatct ggaaactgga atgatagttg aaggaattaa
     1321 aaataataaa attttagttg aggatgaata aaatgtcagc ttttataact tttgagggcc
     1381 cagaaggctc aggtaaaaca actgtaatta atgaagttta tcatagatta gtaaaagatt
     1441 atgatgtcat tatgacaaga gaaccgggcg gcgttcctac tggtgaagaa atacgtaaaa
     1501 ttgtattaga aggcaatgat atggacatta gaactgaagc gatgttattt gctgcatcta
     1561 gaagagaaca tcttgtatta aaggtcatac cagctttaaa agaaggtaag gttgtgttgt
     1621 gtgatcgcta tatcgatagt tcattagctt atcaaggtta tgctagaggg attggcgttg
     1681 aagaagtaag agcattaaat gaatttgcaa taaatggatt atatccagat ttgacgattt
     1741 atttaaatgt tagtgctgaa gtaggtcgcg aacgtattat taaaaattca agagatcaaa
     1801 atagattaga tcaagaagat ttaaagtttc acgaaaaagt aattgaaggt taccaagaaa
     1861 tcattcataa tgaatcacaa cggttcaaaa gcgttaatgc agatcaacct cttgaaaatg
     1921 ttgttgaaga cacgtatcaa actatcatca aatatttaga aaagatatga tataattgtt
     1981 agaagaggtg ttataaaatg aaaatgatta tagcgatcgt acaagatcaa gatagtcagg
     2041 aacttgcaga tcaacttgtt aaaaataact ttagagcaac aaaattggca acaacaggtg
     2101 ggtttttaag agcaggtaat acaacattct tatgtggtgt caatgatgac cgtgtagatg
     2161 aaatattgtc tgtgattaat caaacgtgtg gtaatagaga acagttggtt tcacctatta
     2221 cacctatggg aggcagtgcg gattcgtaca ttccatatcc agttgaagtt gaagttggcg
     2281 gtgctactgt atttgttatg ccagttgatg cattccatca attttaattc tataatacaa
     2341 tcatcaatta gagatactta aaatagtgta ttaataagtt attaacaatt ttgggttgct
     2401 tgactgcgac tagttcagat gccaatagat ttgatttttg tggttctaag aataatcaca
     2461 aatcatggtc gctattgttg cagtaattag ttgctctttg gcaacctttt tatataaaag
     2521 caaaagggag tttgtaatga atggatgaac agcaacaatt gacgaatgca tatcattcaa
     2581 ataaattatc gcatgcctat ttatttgaag gtgatgatgc acaaacgatg aaacaagttg
     2641 cgattaattt tgcaaagctt attttatgtc aaacagatag tcaatgtgaa acaaaggtta
     2701 gtacatataa tcatccagac tttatgtata tatcaacaac tgagaatgca attaagaaag
     2761 aacaagttga acaacttgtg cgtcatatga atcaacttcc tatagaaagc acaaataaag
     2821 tgtacatcat tgaagacttt gaaaagttaa ctgttcaagg ggaaaacagt atcttgaaat
     2881 ttcttgaaga accaccggac aatacgattg ctattttatt gtctacaaaa cctgaacaaa
     2941 ttttagacac aatccattca aggtgtcagc atgtatattt caagcctatt gataaagaaa
     3001 agtttataaa tagattagtt gaacaagaca tgtctaagcc agtagctgaa atgattagta
     3061 cttatactac gcaaatagat aatgcactgg ctttaaatga agaatttgat ttattagcat
     3121 taaggaaatc agttatacgt tggtgtgaat tgttgcttac taataagcca atggcactta
     3181 taggtattat tgatttattg aaacaggcta aaaataaaaa actgcaatct ttaactattg
     3241 cagctgtgaa tggtttcttc gaagatatca tacatacaaa ggtaaatgta gaggataaac
     3301 aaatatatag tgatttaaaa aatgatagtg atcaatatgc gcaaaagttg tcgtttaatc
     3361 aattaatttt gatgtttgat caactgacgg aagcacataa gaaattgaat caaaatgtaa
     3421 atccaacgct tgtatttgaa caaatcgtaa ttaagggtgt gagttagatg ccaaatgtaa
     3481 taggtgttca gtttcaaaaa gcgggaaaat tagaatatta tacacctaat gatatacaag
     3541 tagagttaga tgactgggta gttgtcgaat ctaaaagagg catagagata ggtattgtta
     3601 aaaatccatt aatggatatt gctgaagagg atgttgtgtt acctcttaaa aatattattc
     3661 gcattgctga tgacaaagat attgataaat ttaattgtaa tgaacgcgat gctgaaaatg
     3721 cattaatact atgtaaagac attgtaagag aacaaggttt ggacatgcgt ttagtcaatt
     3781 gcgaatatac attagataaa tcgaaagtta tttttaattt tacggcggat gatcgtattg
     3841 attttagaaa attagtaaaa atattagcgc aacatttaaa aacacgtatc gagttgagac
     3901 aaattggtgt aagggatgaa gccaaattgc ttggcggtat cggaccttgt ggtagatcgt
     3961 tatgttgttc tacattttta ggtgattttg aaccagtatc gattaagatg gctaaggatc
     4021 aaaatttatc attaaatcca actaaaattt ccggtgcatg tggtcgtttg atgtgttgtt
     4081 taaaatatga aaatgactat tatgaggaag tacgtgcgca attacctgat atcggtgaag
     4141 caattgaaac gcctgatggt aacgggaaag tagttgcttt aaatatatta gacatttcta
     4201 tgcaggtgaa gcttgaggga catgaacagc cacttgaata taaattagaa gaaatagaaa
     4261 ctatgcatta aggaggcatt attacatttg gatcgcaatg aaatatttga aaaaataatg
     4321 cgtttagaaa tgaatgtcaa tcaactttca aaggaaactt cagaattaaa ggcacttgca
     4381 gttgaattag tagaagaaaa tgtagcgctt caacttgaaa atgataattt gaaaaaggtg
     4441 ttgggcaatg atgaaccaac tactattgat actgcgaatt caaaaccagc aaaagctgtg
     4501 aaaaagccat taccaagtaa agataatttg gctatattgt atggagaagg atttcatatt
     4561 tgtaaaggcg aattatttgg aaaacatcga catggtgaag attgtctgtt ctgtttagaa
     4621 gttttaagtg attaatcaag cacactcaaa tagtgttata attataaatg aatatggttt
     4681 ggataagtct gagacaatgc atgtttcagg ctttaattgt gtataaagtt ttggtgattg
     4741 cataagagat agcggtacta aatgttatta ttaagtgtgc acgcagtatc attagttata
     4801 aaatgtagct gttaaaagtc aaaaatacat cgaatgtaga taggcatata atataaaaag
     4861 agatttcaat tactcaatag aaaaaggttg tcttcatagg agttaaaaat gttaaaagag
     4921 aatgaacgat ttgatcaact aatcaaagaa aattttagta ttattcaaaa tgatgatgtt
     4981 ttttcgtttt caacggatgc tttgttgtta ggacatttta caaaacctag aacaaaagat
     5041 atagtgttgg acttatgttc aggcaatggg gtgataccct tgttattgtt tgcgaaacat
     5101 ccacgacata tagaaggtgt tgagattcaa aaaacacttg tcgatatggc gcgacgcaca
     5161 tttcaattca atgatgttga tgaatattta acaatgcatc acatggattt gaaaaacgtt
     5221 actaaagtat ttaaaccttc acaatatact ttagtaacgt gtaatccgcc ttattttaaa
     5281 gagaatcagc aacaccaaca tcaaaaagaa gcacataaga tagcgagaca tgagattatg
     5341 tgtacacttg aagattgcat gattgcagcc cgtcatttat taaaagaagg tggcaggcta
     5401 aatatggtac atcgtgcaga tagactcatg gatgtcttgt ttgaaatgag aaaagtaaat
     5461 attgaaccta agaaagtcgt ttttatatat agtaaagtag ggaaatcggc acaaacgata
     5521 gttgtagaag gtcgaaaagg tggaaatcaa ggtttagaaa tcatgccccc attttatatt
     5581 tataatgaag atggtaatta tagcgaagaa atgaaggaag tatattatgg atagtcattt
     5641 tgtatatatt gtaaaatgta gtgatggaag tttatataca ggatacgcta aagacgttaa
     5701 tgcacgtgtt gaaaaacata accgaggtca aggagccaaa tatacgaaag taagacgtcc
     5761 ggtgcattta gtttatcaag aaatgtatga gacaaagtct gaagcattga agcgtgaata
     5821 tgaaattaaa acttatacca gacaaaagaa attgcgatta attaaggagc gatagtatgg
     5881 ctgtattata tttagtgggc acaccaattg gtaatttagc agatattact tatagagcag
     5941 ttgatgtatt gaaacgtgtt gatatgattg cttgtgaaga cactagagta actagtaagc
     6001 tgtgtaatca ttatgatatt ccaactccat taaagtcata tcacgaacat aacaaagata
     6061 agcagactgc ttttatcatt gaacagttag aattaggtct tgacgttgcg ctcgtatctg
     6121 atgctggatt gcccttaatt agtgatcctg gatacgaatt agtagtggca gccagagaag
     6181 ctaacattaa agtagagact gtgcctggac ctaatgctgg gctgacggct ttgatggcta
     6241 gtggattacc ttcatatgta tatacatttt taggattttt gccacgaaaa gagaaagaaa
     6301 aaagtgctgt attagagcaa cgtatgcatg aaaatagcac attaattata tacgaatcac
     6361 cgcatcgtgt gacagataca ttaaaaacaa ttgcaaagat agatgcaaca cgacaagtat
     6421 cactagggcg tgaattgact aagaagttcg aacaaattgt aactgatgat gtaacacaat
     6481 tacaagcatt gattcagcaa ggcgatgtac cattgaaagg cgaatttgtt atcttgattg
     6541 agggtgctaa agcgaataat gagatatcgt ggtttgatga tttatctatc aatgagcatg
     6601 ttgatcatta tattcaaact tcacagatga aaccaaaaca agctattaaa aaagttgctg
     6661 aagaacgaca acttaaaacg aatgaagtat ataatattta ccatcaaata aattaatcac
     6721 tttatcgatt atatgaaatt ttaaacgatt ttataaacgc aagctgtaat tttaaatggt
     6781 aagttatcat tttgcattga tactgataaa atgatgttga ctatgataaa aaaatgatga
     6841 catcgacgtt ttttaatgta aaataaatac attgaaagta ataaatacct taacattgaa
     6901 taagatgaaa atgagatgac gagataaatg ttcgcgtccg ttgaaatgca tagaaatctt
     6961 agatattatt tgaagtgaga cattacgagg aggaacagtt atggctaaag aaacatttta
     7021 tataacaacc ccaatatact atcctagtgg gaatttacat ataggacatg catattctac
     7081 agtggctgga gatgttattg caagatataa gagaatgcaa ggatatgatg ttcgttattt
     7141 gactggaacg gatgaacacg gtcaaaaaat tcaagaaaaa gctcaaaaag ctggtaagac
     7201 agaaattgaa tatttggatg agatgattgc tggaattaaa caattgtggg ctaagcttga
     7261 aatttcaaat gatgatttta tcagaacaac tgaagaacgt cataaacatg tcgttgagca
     7321 agtatttgaa cgtttattaa agcaaggtga tatctattta ggtgaatatg aaggttggta
     7381 ttctgttccg gatgaaacat actatacaga gtcacaatta gtagacccac aatacgaaaa
     7441 cggtaaaatt attggtggta aaagtccaga ttctggacat gaagttgaac tagtaaaaga
     7501 agaaagttat ttctttaata ttagtaaata tacagatcgt ttattagagt tctatgacca
     7561 aaatccagat tttatacaac caccatcaag aaaaaatgaa atgattaaca acttcattaa
     7621 accaggactt gctgatttgg ctgtttctcg tacatcattt aactggggtg tccatgttcc
     7681 gtctaatcca aaacatgttg tttatgtttg gattgatgcg ttagttaact atatttcagc
     7741 attaggctat ttatcagatg atgagtcact atttaacaaa tactggccag cagatattca
     7801 tttaatggct aaggaaattg tgcgattcca ctcaattatt tggcctattt tattgatggc
     7861 attagactta ccgttaccta aaaaagtctt tgcacatggt tggattttga tgaaagatgg
     7921 aaaaatgagt aaatctaaag gtaatgtcgt agacccgaat attttaattg atcgctatgg
     7981 tttagatgcc acacgttatt atctaatgcg tgaattacca tttggttcag atggcgtatt
     8041 tacacctgaa gcatttgttg agcgtacaaa tttcgatcta gcaaatgact taggaaactt
     8101 agtaaaccgt acgatttcta tgattaataa gtactttgat ggcgaattac cagcgtatca
     8161 aggtccactt catgaattag atgaagaaat ggaagctatg gctttagaaa cagtgaaaag
     8221 ttatactgaa agcatggaaa gtttgcaatt ttctgtggca ttatctacgg tatggaagtt
     8281 tattagtaga acgaataagt atattgacga aacaacgcct tgggtattag ctaaggacga
     8341 tagccaaaaa gatatgttag gcaatgttat ggctcactta gttgaaaata ttcgttatgc
     8401 agctgtatta ttgcgtccat tcttaactca tgcgccgaaa gagatttttg aacaattgaa
     8461 cattaacaat cctcaattta tggaatttag tagtttagag caatatggtg tgcttactga
     8521 gccaattatg gttactgggc aacctaaacc tattttccca agattggata gcgaagcgga
     8581 aattgcatat atcaaagaat caatgcaacc gcctgctact gaagaggaaa aagaagagat
     8641 tcctagcaaa cctcaaattg atattaaaga ctttgataaa gttgaaatta aggcagcaac
     8701 gattattgat gctgaacatg ttaagaagtc agataagctt ttaaaaattc aagtagactt
     8761 agattctgaa caaagacaaa tcgtatcagg aattgccaaa ttctatacac cagatgatat
     8821 tattggtaaa aaagtagcag ttgttactaa cttgaaacca gctaaattaa tgggacaaaa
     8881 atctgaaggt atgatattat ctgctgaaaa agatggtgta ttaaccttag taagtttacc
     8941 aagtgcaatt ccaaatggtg cagtgattaa ataactgtat ttttaaaaat taggagagat
     9001 aattatgtta atcgatacac atgtccattt aaatgatgag caatacgatg atgatttgag
     9061 tgaagtgatt acacgtgcta gagaagcagg tgttgatcgt atgtttgtag ttggttttaa
     9121 caaatcgaca attgaacgcg cgatgaaatt aatcgatgag tatgattttt tatatggcat
     9181 tatcggttgg catccagttg acgcaattga ttttacagaa gaacacttgg agtggattga
     9241 atctttagct cagcatccaa aagtgattgg tattggtgaa atgggattag attatcactg
     9301 ggataaatct cctgcagatg ttcaaaagga agtttttaga aagcaaattg ctttagctaa
     9361 gcgtttgaag ttaccaatta tcattcataa ccgtgaagca acacaagact gtatcgatat
     9421 cttattggag gagcatgctg aagaggtagg cgggattatg catagcttta gtggttctcc
     9481 agaaattgca gatattgtaa ctaataagct gaatttttat atttcattag gtggaccagt
     9541 gacatttaaa aatgctaaac agcctaaaga agttgctaag catgtgtcaa tggagcgatt
     9601 gcttgttgaa accgatgcac cgtatctttc gccacatcca tatagaggga agcgaaatga
     9661 accggcgaga gtaactttag tagctgaaca aattgctgaa ttaaaaggct tatcttatga
     9721 agaagtgtgc gaacaaacaa ctaaaaatgc agagaaattg tttaatttaa attcataaag
     9781 ttaaaagtga gaaagatcac cgccataaat gtaaacgatg ctatattcgt ttaatatgct
     9841 atggttcttt ctcacttttt taaattaaaa tatcgtgcat gtgcaatacg tgcgatagag
     9901 atggttagag ctttgaaatt aagaattgta ggaaggcgtt ttaaatgaaa atcaatgagt
     9961 ttatagttgt agaaggacga gatgatactg agcgtgttaa acgagctgtt gaatgtgata
    10021 cgattgaaac gaatggtagt gccatcaacg aacaaacttt agaagtaatt agaaatgctc
    10081 aacaaagtcg aggcgttatt gtattaacag atccagattt cccaggagat aaaattagaa
    10141 gtacaattac tgaacatgtc aaaggtgtta aacatgcgta tattgataga gaaaaagcta
    10201 aaaataaaaa agggaaaatt ggtgttgaac atgccgactt aattgatatt aaagaagcgt
    10261 taatgcatgt tagttcaccc tttgatgaag cttatgaatc aattgataaa tctgtgctaa
    10321 tagagttggg gttaatcgtt gggaaagatg caaggcgccg tagagaaatt ttaagtagaa
    10381 aattgcgaat cggccattcc aatggtaagc agttattgaa aaagttaaat gcatttggtt
    10441 ataccgaagc ggatgtaagg caagctttag aagatgaatg aggaagtgaa aatgttggat
    10501 aataaagata ttgcaacacc atcaagaacg cgagcgttgt tagataaata tggctttaat
    10561 tttaaaaaaa gtttaggaca gaacttttta atagatgtga atatcattaa taatatcatt
    10621 gatgcaagtg atattgatgc acaaactggg gtgattgaaa ttggtccagg tatggggtcg
    10681 ttgacagaac aattggccag acatgctaaa agagtattgg catttgaaat tgatcaacga
    10741 ttaataccgg tattaaatga tacactatca ccttatgata atgtgacggt gattaatgaa
    10801 gatattttaa aagcgaatat taaagaagct gttgaaaatc atttacaaga ttgcgaaaaa
    10861 ataatggttg ttgcaaacct gccgtactat attacgacgc caattttatt aaatttaatg
    10921 caacaagata taccaattga tggctacgtg gtgatgatgc aaaaagaagt gggcgaacgc
    10981 ttaaatgctg aaataggttc aaaagcatat ggttcattat caattgtcgt acaatactat
    11041 acagagacta gtaaagtatt aacggtacct aaatctgtat ttatgccacc acctaatgtt
    11101 gattcaatag ttgtaaaact gatgcagaga actgaaccgt tagtaacagt agataacgag
    11161 gaagtattct ttaagttagc aaaagcagca tttgcacaaa gaagaaagac aattaacaat
    11221 aactatcaaa attattttaa agatggtaaa caacacaaag aagtgattat acaatggtta
    11281 gaacaagcgg gtattgatcc aagacgtcgc ggtgaaacgc tatctattca agattttgct
    11341 aaattgtatg aagaaaagaa aaaattccct caattagaaa attaaatgat tgacaaagca
    11401 aagcactatt gttaaaattt aaattttgtt tgacgaaaac gctgcaaata tggtattatg
    11461 taacttgtag cgaggtggag caatatgcca aaatcaattt tggacatcaa aaattctatt
    11521 gattgtcatg taggaaatcg tattgtactg aaagccaatg gaggccgtaa gaaaacaata
    11581 aaacgttctg gaattttaaa agaaacatat ccgtcagttt tcattgttga gttagatcaa
    11641 gacaaacaca actttgagag agtatcttat acatacactg atgtgttaac tgaaaatgtt
    11701 caagtttcat ttgaagagga taatcatcac gaatcaattg cacactaaat aagacatata
    11761 gagatgttag acgtttctta gtataagaag taaatgttat gataattatt tgagtgttgg
    11821 gcattatgtt caatactctt tttatttaca aaatgtttaa cactgatgtt tcgcttatag
    11881 atttttcagt aaatggataa ttgtatttat aaacacaaat acaagtaaat actaagtaat
    11941 tagatggaga aaattacttt tttattaaaa aaacactaaa aaacaaatta aaatgtcaaa
    12001 tattaattct ctttatgtta aaatcatcat attaagataa cgaaaagagg gcgcaaaaat
    12061 gatatatgaa acggcaccag ccaaaattaa ttttacgctc gatacacttt ttaaaagaaa
    12121 tgatggctat catgagattg aaatgataat gacaacagtt gatttaaatg atcgtttaac
    12181 ttttcataaa agaaaagatc gaaagatagt tgttgagatt gaacataatt atgtgccttc
    12241 taatcataaa aatctcgcat atcgtgcagc gcaactattt attgagcaat atcaactaaa
    12301 gcaaggtgta acaatttcta tcgataaaga aatacctgtt tctgctggct tagctggagg
    12361 ttcggctgat gcagcagcaa cgttaagagg attgaatcga ctttttaata taggggcgag
    12421 tttggaagaa ttggctctac taggcagtaa aatcgggaca gatattccgt tttgtattta
    12481 taataaaact gcactatgta ctggaaaagg agaaaaaatc gagtttttaa ataaaccacc
    12541 ttcagcttgg gtgattcttg ctaaaccaaa cttaggcata tcatcaccag atatatttaa
    12601 gttgattaat ttagataagc gttacgacgt acatacgaaa atgtgttatg aggccttaga
    12661 aaatcgagat tatcaacaat tatgtcaaag tttgtctaat cgattagagc caatttctgt
    12721 ttcaaaacac ccacaaatcg ataaattaaa aaacaatatg ttgaaaagtg gtgcagatgg
    12781 tgcgttaatg agtggaagcg gaccgactgt gtatgggcta gcacaaaaag aaagccaagc
    12841 aaaaaatatt tataatgcag ttaacggttg ttgtaatgaa gtgtacttag ttagactatt
    12901 aggatagaag ggttgaaaag atgagatata aacgaagcga gagaattgtt tttatgacgc
    12961 aatatttgat gaaccatccg aataaattga ttccattaac tttttttgtg aaaaaattta
    13021 aacaggcgaa gtcttcaata agtgaagatg tccaaattat aaaaaataca ttccaaaaag
    13081 aaaagttagg tacagtaatt actactgctg gcgcaagtgg tggtgttacg tataaaccaa
    13141 tgatgagtaa agaagaggcg actgaagttg ttaatgaggt cattactcta ttagaagaga
    13201 aagaacgttt gttacctggc gggtatttat ttttatcaga tttggtaggt aatccatcgc
    13261 tactaaacaa agttggtaag ttaattgcca gtatttacat ggaagaaaaa ttagatgctg
    13321 ttgttaccat tgcgacaaaa ggtatttcat tggcaaatgc ggttgctaat attttaaatt
    13381 taccagtagt agtgattaga aaagacaaca aggtgactga aggttctaca gtttcaatta
    13441 attacgtttc aggatcttca agaaaaatag agacaatggt actttcgaag agaactttag
    13501 cagaaaattc aaatgtttta gttgtcgatg attttatgag ggctggtggc tctatcaatg
    13561 gtgttatgaa tttaatgaat gagtttaaag cccatgtaaa aggggtatca gtacttgtag
    13621 aatcaaaaga agttaaacaa agattgattg aagattatac ttccttagtg aaattatctg
    13681 atgtagatga atataatcaa gagtttaacg tagaacctgg caacagttta tctaagtttt
    13741 cataaaagga gttttagtat tatgaaaatc attaacacaa caagattacc ggaagcactt
    13801 ggaccatatt cgcatgcaac agttgtgaat ggtatggttt atacttctgg tcaaattcca
    13861 ttgaatgttg atgggaaaat cgtaagcgct gatgttcaag cacaaacaaa acaagtttta
    13921 gaaaatttaa aggttgtttt ggaagaagca ggatctgatt tgaattctgt tgcgaaagcg
    13981 accattttca ttaaagatat gaatgatttc caaaaaataa atgaagtgta tggtcaatat
    14041 tttaatgaac acaagccagc gcgtagttgt gtagaggttg cgcgtttgcc aaaagatgtg
    14101 aaagtagaaa ttgaattagt aagtaaaatt aaggaattat aattttcgat taatatgttt
    14161 aatcaagctt ctaaataaaa cagagagaga tatactatag ggggctcact acatgaaagt
    14221 gacagatgta agacttagaa aaatacaaac agatggacga atgaaagcac tcgtttccat
    14281 tacattagat gaagctttcg taattcatga tttacgtgta attgaaggaa actctggctt
    14341 gttcgttgca atgccaagta aacgtacacc agatggtgaa ttccgcgaca tcgcgcatcc
    14401 tattaattca gatatgagac aagaaattca agatgcagtg atgaaagtat atgatgaaat
    14461 agatgaagta gtaccagata aaaacgctac atcagaagat tcagaagaag cttaatcaat
    14521 tttatattta gcgatgtaat acatttgcaa taagttgatt tgatactgtc gataaagcat
    14581 aaagctttgt cggcagtttt tttagtttgt attaatatta attttatgaa atgaaaggct
    14641 aataaatata tatgttaaca gattatgatg atatgaaaat tattgataca ttgctgataa
    14701 aaattgcgtt tgaatgatgc tcgtattttt gaagtaagga aaaagttgtt tttaaaatta
    14761 caacgaatta aaaacaatgc cttttatatg ttgaaagagt attgcagatt aaattataat
    14821 aatgacgaag tgtaaaattt aatggggggt taatgttcat gcgaagacac gcgataattt
    14881 tggcagcagg taaaggcaca agaatgaaat ctaaaaagta taaagtgcta cacgaggttg
    14941 ctggaaaacc tatggtcgaa catgtattgg aaagtgtgaa aggctctggt gtcgatcaag
    15001 ttgtaaccat cgtaggacat ggtgctgaaa gtgtaaaagg acatttaggc gagcgttctt
    15061 tatacagttt tcaagaggaa caactcggta ctgcgcatgc agtgcaaatg gcgaaatcac
    15121 acttagaaga caaggaaggt acgacgatcg ttgtatgtgg tgacacaccg ctcatcacaa
    15181 aggaaacatt agaaacattg attgcgcatc acgaggatgc taatgctcaa gcaactgtat
    15241 tatctgcatc gattcaacaa ccatatggat acggaagaat cgttcgaaat gcgtcaggtc
    15301 gtttagaacg catagttgaa gagaaagatg caacgcaagc tgaaaaggat attaatgaaa
    15361 ttagttcagg tatttttgcg tttaataata aaacgttgtt tgaaaaatta acacaagtga
    15421 aaaatgataa tgcgcaaggt gaatattacc tccctgatgt attgtcgtta attttaaatg
    15481 atggcggcat cgtagaagtc tatcgtacca atgatgttga agaaatcatg ggtgtaaatg
    15541 atcgtgtaat gcttagtcag gctgagaatg cgatgcaacg tcgtacgaat cattatcaca
    15601 tgctaaatgg tgtgacaatc atcgatcctg acagtactta tattggtcca gacgttacaa
    15661 ttggtagtga tacagtcatt gaaccaggcg tacgaattaa tggtcgtaca gaaattggcg
    15721 aagatgttgt tattggtcag tactctgaaa ttaacaatag tacgattgaa aatggtgcat
    15781 gtattcaaca gtctgttgtt aatgatgcta gcgtaggagc gaatactaag gtcggaccgt
    15841 ttgcgcaatt gagaccaggc gcgcaattag gtgcagatgt taaggttgga aattttgtag
    15901 aaattaaaaa agcagatctt aaagatggtg ccaaggtttc acatttaagt tatattggcg
    15961 atgctgtaat tggcgaacgt actaatattg gttgcggaac gattacagtt aactatgatg
    16021 gtgaaaataa atttaaaact atcgtcggca aagattcatt tgtaggttgc aatgttaatt
    16081 tagtagcacc tgtaacaatt ggtgatgatg tattggtggc agctggttcc acaatcacag
    16141 atgacgtacc aaatgacagt ttagctgtgg caagagcaag acaaacaaca aaagaaggat
    16201 ataggaaata atcatttacg tatttaaaat ggctaggata aaaggataat cctatgtaat
    16261 attaatgtaa tctttatgat ttaatgattc gcatagtaat ggagttacat tttatatata
    16321 atagtaattg cgtaagtaaa taattggagg actataaatg ttaaataatg aatataagaa
    16381 ttcgtcatta aagatttttt cattgaaagg aaacgaagca ttagcgcaag aagttgctga
    16441 ccaagtagga attgaactag gtaaatgttc agttaaacgt tttagtgatg gagaaattca
    16501 aattaatatc gaagagagta ttcgtggttg tgacgtattt attattcaac caacatcata
    16561 tcctgtgaat ctacatttaa tggaattatt aattatgatt gatgcttgta aacgtgcttc
    16621 tgcagcaaca atcaatattg tagtgccata ttatggatat gcaagacaag atagaaaagc
    16681 ccgtagccgt gagccaatca ctgctaaatt agttgcaaac ttaatcgaaa cagctggcgc
    16741 aactcgtatg attgcgttag acttacatgc accacaaatt caaggattct ttgatattcc
    16801 aattgaccac ttaatgggtg tgccaattct tgctaaacat ttcaaagatg atccgaatat
    16861 taacccagaa gaatgtgtcg ttgtttcacc agaccatggc ggtgttacac gtgcacgtaa
    16921 attagctgac attttaaaaa ctccaattgc aattatagat aaacgtcgtc ctagaccaaa
    16981 tgttgctgaa gtgatgaaca ttgttggtga gattgaagga cgtacggcaa ttattattga
    17041 cgatattatt gatacagcag gtacaatcac tttagctgca caagcattaa aagataaagg
    17101 tgctaaagaa gtatatgctt gttgtacaca ccctgtttta tcaggaccgg ctaaagaacg
    17161 tatcgaaaat tctgctataa aagaattaat cgtaacaaac tcaattcatt tagatgaaga
    17221 tcgcaaacca tctaacacta aagaattatc tgttgctggt ttaatcgcac aagctatcat
    17281 tcgtgtatac gaaagagaat cagttagcgt attatttgac taatatttaa aaggcgtttg
    17341 acgaacatat tccaaacgtg tataatagtt tcgttcgtga ttatacgaat aaataaacac
    17401 ttgcaagcaa cgatgatgtt gatgggtaag tgaggtgctc gttttgagca aaaatgaaag
    17461 gtggaaatga gaatggcttc attaaagtca atcatccgtc aaggtaaaca aacacgttca
    17521 gatcttaaac aattaagaaa atctggtaaa gtaccagcag tagtatacgg ttacggtact
    17581 aaaaacgtgt cagttaaagt tgatgaagta gaattcatca aagttatccg tgaagtaggt
    17641 cgtaacggtg ttatcgaatt aggcgttggt tctaaaacta tcaaagttat ggttgcagac
    17701 taccaattcg atccacttaa aaaccaaatt actcacattg acttcttagc aatcaatatg
    17761 agtgaagaac gtactgttga agtaccagtt caattagttg gtgaagcagt aggcgctaaa
    17821 gaaggcggcg tagttgaaca accattattc aacttagaag taactgctac tccagacaat
    17881 attccagaag caatcgaagt agacattact gaattaaaca ttaacgacag cttaactgtt
    17941 gctgatgtta aagtaactgg cgacttcaaa atcgaaaacg attcagctga atcagtagta
    18001 acagtagttg ctccaactga agaaccaact gaagaagaaa tcgaagctat ggaaggcgaa
    18061 caacaaactg aagaaccaga agttgttggc gaaagcaaag aagacgaaga gaaaactgaa
    18121 gagtaagttt aactgattac cataaagttt ttatactttg ttaaacaagc actgtgctta
    18181 ttttaatata agcatggtgc tttttgtgtt attataaagc ttaattaaac tttattgctt
    18241 tgtactaaag ttaaattaat tttagtgagt aaaagacatt aaactcaaca atgatacatc
    18301 ataaaaattt taatgtactt gattttaaaa tacatactta ctaatctaaa gaataatgat
    18361 aattgatggc aatggcggaa aatagatgtt gtcattataa taataaatga aacaattatg
    18421 ttggaggtaa acacgcatga aatgtattgt aggtctaggt aatataggta aacgttttga
    18481 acttacaaga cataatatcg gctttgaagt cgttgattat attttagaga aaaataattt
    18541 ttcattagat aaacaaaagt ttaaaggtgc atatacaatt gaacgaatga acggagataa
    18601 agtgttattc atcgaaccaa tgacaatgat gaatttgtca ggtgaagcag ttgcaccgat
    18661 tatggattat tacaatgtta atccagaaga tttaattgtc ttatatgatg atttggattt
    18721 agaacaagga caagttcgct taagacaaaa aggaagtgcg ggcggtcaca atggtatgaa
    18781 atcaattatt aaaatgcttg gtacagacca atttaaacgt attcgtattg gtgtgggaag
    18841 accaacgaat ggtatgacgg tacctgatta tgttttacaa cgcttttcaa atgatgaaat
    18901 ggtaacgatg gaaaaagtta tcgaacacgc agcacgcgca attgaaaagt ttgttgaaac
    18961 atcacgattt gaccatgtta tgaatgaatt taatggtgaa gtgaaataat gacaatattg
    19021 acaacgctta taaaagaaga taatcatttt caagacctta atcaggtatt tggacaagca
    19081 aacacactag taactggtct ttccccgtca gctaaagtga cgatgattgc tgaaaaatat
    19141 gcacaaagta atcaacagtt attattaatt accaataatt tataccaagc agataaatta
    19201 gaaacagatt tacttcaatt tgtagatgct gaagaattgt ataagtatcc tgtgcaagat
    19261 attatgaccg aagagttttc aacacaaagc cctcaactga tgagtgaacg tattagaact
    19321 ttaactgcgt tagctcaagg taagaaaggg ttatttatcg ttcctttaaa tggtttgaaa
    19381 aagtggttaa ctcctgttga aatgtggcaa aatcaccaaa tgacattgcg tgttggtgag
    19441 gatatcgatg tggaccaatt tcttaacaaa ttagttaata tggggtacaa gcgggaatct
    19501 gtggtatcgc atattggtga attctcattg cgaggaggta ttatcgatat ctttccgcta
    19561 attggggaac caatcagaat tgagctattt gataccgaaa ttgattctat tcgggatttt
    19621 gatgttgaaa cgcagcgttc caaagataat attgaagaag tcgatatcac aactgcaagt
    19681 gattatatca ttactgaaga agtaatcagc catcttaaag aagagttaaa aactgcatat
    19741 gaaaatacaa gacccaaaat agataaatca gtgcgcaatg atctgaaaga aacgtatgaa
    19801 agctttaaat tattcgaaag tacatacttt gatcatcaaa tactacgtcg tttagtagcg
    19861 tttatgtatg aaacaccttc gacaattatt gattatttcc aaaaagatgc aatcattgcg
    19921 gttgatgaat ttaatcgtat taaagaaact gaagaaagtt taacagtaga gtctgattcg
    19981 tttattagca acgttattga aagtggtaat ggatttatag gacaaagttt tataaaatat
    20041 gatgattttg aaacattgat tgaaggctat cctgtcactt atttttcatt attcgctaca
    20101 acaatgccga taaaactaaa tcatattatt aaattttcat gtaaacctgt ccaacaattt
    20161 tatgggcaat atgacattat gcgttctgaa tttcaacgat atgttaatca aaattatcat
    20221 atcgtggttt tggtcgaaac cgaaactaaa gttgaacgta tgcaagcgat gttaagtgaa
    20281 atgcatattc catcaataac aaaattgcat cgctcaatgt catcggggca agcagtgatt
    20341 attgaaggca gtttatctga aggatttgaa ctacctgata tgggattagt tgtcattact
    20401 gaacgtgagc tttttaaatc aaaacagaaa aagcaacgaa aacgtacgaa agctatctca
    20461 aatgctgaaa aaattaagtc ttaccaagat ttaaatgtgg gagattatat tgttcatgtg
    20521 catcatggtg ttggtagata tttaggtgtt gagacgctcg aagtagggca aatacatcgt
    20581 gattatatta aattgcaata taaaggtacg gatcaactat ttgttccagt agatcaaatg
    20641 gatcaagttc aaaaatatgt agcttcggaa gataagacgc caaaattaaa taaactcggt
    20701 ggcagtgaat ggaaaaaaac aaaagctaaa gttcaacaaa gtgttgaaga tattgctgaa
    20761 gagttgattg atttatataa agaaagagaa atggcagaag gttatcaata tggggaagac
    20821 acagctgagc aaacaacatt tgaattagat tttccatatg aacttacgcc tgaccaagcg
    20881 aaatctatcg atgaaattaa agatgacatg caaaaatcgc gtccaatgga tcgcttgcta
    20941 tgtggtgatg ttggttatgg taaaactgaa gttgcagtga gagcagcatt caaagctgta
    21001 atggaaggaa agcaggttgc atttttagtt cctacaacta ttttagctca gcaacattat
    21061 gagacgttaa ttgagcgtat gcaagatttt cctgttgaaa ttcaattaat gagtcgtttt
    21121 agaacgccaa aagagataaa acaaactaag gaaggactta aaactgggtt tgttgacata
    21181 gttgttggta cacacaaatt acttagtaaa gatatacagt ataaagattt agggctgttg
    21241 attgtagatg aagaacaacg atttggtgta cgccataaag agcgtattaa aacattaaaa
    21301 cataatgtag atgtactaac attgactgca accccaatac ctagaacatt gcatatgagt
    21361 atgctaggtg tgcgcgattt gtcagtgatt gaaacgccgc cagaaaatcg tttcccagtt
    21421 caaacatatg tattagaaca gaacatgagt tttatcaaag aagctttaga aagagaacta
    21481 tcccgtgatg gccaagtatt ttatctttat aataaagtgc aatccattta tgaaaaacga
    21541 gaacaactcc agatgttaat gccagatgct aacattgcag ttgctcatgg acaaatgaca
    21601 gagcgcgatt tagaagaaac gatgttaagt tttatcaata atgaatatga tattttagta
    21661 acgacgacga ttattgaaac aggtgtcgat gtcccaaatg caaatacttt gatcattgaa
    21721 gatgcagatc gctttggatt gagtcagtcg tatcaattaa gaggtcgtgt tggtcgttca
    21781 agtcgtattg gttatgcata cttcttacat ccagcaaata aggtactaac tgagactgca
    21841 gaagatcgat tacaagcgat taaagaattt acggagttag gctcaggatt taagattgcg
    21901 atgcgtgatt tgaacattcg tggtgctggt aatttgttag gtaaacaaca gcacggcttt
    21961 attgatacag ttggatttga tttgtacagt caaatgttag aagaagctgt aaatgaaaaa
    22021 cgtggtatta aggaaccaga atctgaggtg ccagaagtcg aagttgattt aaacttggat
    22081 gcatatttgc caacagaata tattgcaaat gaacaagcta aaattgaaat ttataaaaag
    22141 ctaagaaaaa ctgaaacatt tgatcaaatt atcgacataa aagatgaatt aattgatcgt
    22201 ttcaatgatt atcctgttga agtagcacgt ttgcttgata tagtggaaat aaaagtacac
    22261 gcattacatt caggtatcac gttgattaaa gataaaggga aaataattga tattcattta
    22321 tctgtaaaag ccactgaaaa tattgatggc gaagtgctgt tcaaagcaac acaaccttta
    22381 ggtagaacaa tgaaggttgg tgttcaaaat aatgcaatga caattacttt aacgaaacaa
    22441 aatcaatggc ttgatagttt gaagttttta gttaagtgca ttgaagaaag tatgagaatc
    22501 agtgatgaag cataaagaag catttaatgg cgttgtcgtg ttaactgctg cattaattgt
    22561 cattaaaatt ctgagtgctg tatatcgaat tccatatcaa aatatattag gcgatacagg
    22621 tttgtatgca tatcaacaag tgtatccaat tgtagcatta ggaatgatat tatcgatgaa
    22681 tgccattcct agtgcaatta cacaaaatat agggaagtat catagtgacg aagcatatgc
    22741 aaaagcagtc gcttatatac aattagttgg tatgttatta tttattgcta tttttgtgtt
    22801 tgcgaacaat attgcacata tgatgggtga tagccattta acacctatga ttcaagctgc
    22861 aagtttaagc tttatattta taggtatgct tggcgtgtta agaggttatt atcaatctgc
    22921 aaataatatg acagttccgg ctatttccca ggttatagaa caagttatac gagtaggtat
    22981 tatcattgtt actattgtta tttttgtaga tagaggttgg acgatatatg aagcgggtac
    23041 aattgctatt ttagcatcaa cgataggttt tttaggttct tcaatttatt tagtagcgca
    23101 ccgacctttt aagtttaaaa tggtaaataa cactgcaaag attgtttgga aacagttcgc
    23161 actttcggtt ttgattttcg ctatcagtca attaatcgta attttatggc aagtgattga
    23221 tagtgttact attattaagt cacttcaagc gatacgcgtg ccattcgatg ttgctataac
    23281 tgaaaaagga gtctatgacc gtggtgcatc atttattcag atgggattga ttgtaactac
    23341 aacatttagt tttgcgctca ttcctctgtt aagtgacgca atcaaaatga ataatcaggt
    23401 acttatgaat cgttatgcaa atgcgtcatt aaagattacg attttaataa gtacagcagc
    23461 gggaatagga ttaattaatt tattgccttt aatgaacggt gtatttttta agacgaatga
    23521 tttaacctta acgttaagcg tttatatgat tacggttatt tgtgtatcgt taattatgat
    23581 ggatatggca ttattacaag ctcaacatgc tgtgagacca atttttgtcg gtatgacggc
    23641 aggattggtt attaaattta tacttaatat cattttgatt cgtttaagtg gcattattgg
    23701 tgcgagcatt agtactgttg tatcattaat tatattcggt acgattatcc atattgctgt
    23761 cacgagaaaa taccatttac atgcgatgag acgatttttt atcaatgttg ttttaggtat
    23821 ggtatttatg tcgattgttg ttcaatgcgt gttaaacata gtgacaacac acggtagaat
    23881 cactggactc gttgaattat tatgtgcagc agtattaggt atcattgcat tgtttttcta
    23941 tatttttaga tttaatgttt tgacatataa agagttaact tatttaccat ttggttcaaa
    24001 gttgtatcaa att
//
LOCUS       DAMZPP010000023        24010 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMZPP010000023 DAMZPP010000000
VERSION     DAMZPP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24010
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.023"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            74..223
                     /gene="rpmG"
                     /locus_tag="POH73_000714"
     CDS             74..223
                     /gene="rpmG"
                     /locus_tag="POH73_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDI6886689.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            432..971
                     /locus_tag="POH73_000715"
     CDS             432..971
                     /locus_tag="POH73_000715"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDI6886690.1"
                     /translation="MTKNEIRKYILHKMKTFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDKKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQSA"
     gene            983..2446
                     /locus_tag="POH73_000716"
     CDS             983..2446
                     /locus_tag="POH73_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDI6886691.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSTIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQNIVKQ
                     TINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLND
                     DEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK"
     gene            2427..2630
                     /locus_tag="POH73_000717"
     CDS             2427..2630
                     /locus_tag="POH73_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDI6886692.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            2627..3613
                     /locus_tag="POH73_000718"
     CDS             2627..3613
                     /locus_tag="POH73_000718"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDI6886693.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            3613..3942
                     /locus_tag="POH73_000719"
     CDS             3613..3942
                     /locus_tag="POH73_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDI6886694.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            3939..4562
                     /locus_tag="POH73_000720"
     CDS             3939..4562
                     /locus_tag="POH73_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6886695.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            4614..5588
                     /locus_tag="POH73_000721"
     CDS             4614..5588
                     /locus_tag="POH73_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDI6886696.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMDKYI"
     gene            5560..6630
                     /locus_tag="POH73_000722"
     CDS             5560..6630
                     /locus_tag="POH73_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDI6886697.1"
                     /translation="MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNKILSLIGYSDTIVLQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
     gene            6644..6955
                     /locus_tag="POH73_000723"
     CDS             6644..6955
                     /locus_tag="POH73_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDI6886698.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEEQKTCKSGETITISNGEAVA
                     N"
     gene            6933..7379
                     /locus_tag="POH73_000724"
     CDS             6933..7379
                     /locus_tag="POH73_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDI6886699.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTLTSKGLSNLRVI
                     DDEANIISLITELNYIKSQAIANQGYINVRFYENSDTIKVIENNKIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIDNNNSIYRIIFHIEKGRIRYEKL"
     gene            7366..7665
                     /locus_tag="POH73_000725"
     CDS             7366..7665
                     /locus_tag="POH73_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HDI6886700.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            7583..8080
                     /locus_tag="POH73_000726"
     CDS             7583..8080
                     /locus_tag="POH73_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDI6886701.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLNKTFLIESRELTTVDFEFFSRDILEDFKGVDRNDIEIRQQRIILH
                     KGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
                     KTIYV"
     gene            8177..8323
                     /locus_tag="POH73_000727"
     CDS             8177..8323
                     /locus_tag="POH73_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886702.1"
                     /translation="MMLTAIYFSIFTFYISQYSLKLKTLYNIETDYNYKIVLLLKEGK
                     THES"
     gene            8313..8837
                     /locus_tag="POH73_000728"
     CDS             8313..8837
                     /locus_tag="POH73_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDI6886703.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFSKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEVFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRILRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      8824..8921
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            8996..10087
                     /gene="gcvT"
                     /locus_tag="POH73_000729"
     CDS             8996..10087
                     /gene="gcvT"
                     /locus_tag="POH73_000729"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDI6886704.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGAIVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            10107..11453
                     /gene="gcvPA"
                     /locus_tag="POH73_000730"
     CDS             10107..11453
                     /gene="gcvPA"
                     /locus_tag="POH73_000730"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDI6886705.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AESEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDDTAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            11446..12918
                     /gene="gcvPB"
                     /locus_tag="POH73_000731"
     CDS             11446..12918
                     /gene="gcvPB"
                     /locus_tag="POH73_000731"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDI6886706.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPISDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSEHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPYTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(13282..13668)
                     /locus_tag="POH73_000732"
     CDS             complement(13282..13668)
                     /locus_tag="POH73_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDI6886707.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            13826..14656
                     /locus_tag="POH73_000733"
     CDS             13826..14656
                     /locus_tag="POH73_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDI6886708.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIELKPLELTEVQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(14720..14938)
                     /locus_tag="POH73_000734"
     CDS             complement(14720..14938)
                     /locus_tag="POH73_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886709.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLVSFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(14952..15533)
                     /locus_tag="POH73_000735"
     CDS             complement(14952..15533)
                     /locus_tag="POH73_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886710.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            15638..16699
                     /locus_tag="POH73_000736"
     CDS             15638..16699
                     /locus_tag="POH73_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDI6886711.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAADIVDETYEYILTVAKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYFNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            16725..17282
                     /gene="efp"
                     /locus_tag="POH73_000737"
     CDS             16725..17282
                     /gene="efp"
                     /locus_tag="POH73_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDI6886712.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
     gene            17756..18220
                     /gene="accB"
                     /locus_tag="POH73_000738"
     CDS             17756..18220
                     /gene="accB"
                     /locus_tag="POH73_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDI6886713.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPFEAAAMPMPQVQSTDNNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            18220..19575
                     /gene="accC"
                     /locus_tag="POH73_000739"
     CDS             18220..19575
                     /gene="accC"
                     /locus_tag="POH73_000739"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDI6886714.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            19590..19952
                     /locus_tag="POH73_000740"
     CDS             19590..19952
                     /locus_tag="POH73_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDI6886715.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            20012..20401
                     /gene="nusB"
                     /locus_tag="POH73_000741"
     CDS             20012..20401
                     /gene="nusB"
                     /locus_tag="POH73_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDI6886716.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKDHEPVLDETISPNLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            20418..21755
                     /locus_tag="POH73_000742"
     CDS             20418..21755
                     /locus_tag="POH73_000742"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDI6886717.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            21748..21978
                     /locus_tag="POH73_000743"
     CDS             21748..21978
                     /locus_tag="POH73_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDI6886718.1"
                     /translation="MTKETQSFEEMMQELERIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            21956..22837
                     /locus_tag="POH73_000744"
     CDS             21956..22837
                     /locus_tag="POH73_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDI6886719.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMNTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDATKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELMQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            23268..23720
                     /gene="argR"
                     /locus_tag="POH73_000745"
     CDS             23268..23720
                     /gene="argR"
                     /locus_tag="POH73_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDI6886720.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            23736..>24010
                     /locus_tag="POH73_000746"
     CDS             23736..>24010
                     /locus_tag="POH73_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6886721.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDF"
BASE COUNT         8876 a         3140 c         4323 g         7671 t
ORIGIN      
        1 tttaactttg caaatattat agatgttggt ataataataa agtcgtattt agaaatgata
       61 aggaggaata accatgcgcg taaacgtaac tttagcttgt acggaatgtg gtgacagaaa
      121 ctacattaca actaagaaca aaagaaataa tccagaacgt gttgaaatga agaaattctg
      181 ttcacgtgaa aacaaacaaa ctttacaccg tgaaacaaaa taatcattaa aattaaattt
      241 aaattaacaa taaaaaaggt gaaatctcaa aataagaggt ttcacctttt taattgtgca
      301 cacgtatgaa atgataaaag attttcaatt gaaataataa attttagaat tgtcgcataa
      361 ttcatcatga caacataatg acatgtattg ttattaacga tataatagaa ttgaataaag
      421 tgggtgataa tgtgactaaa aatgagatta gaaaatatat tttacataaa atgaagactt
      481 ttaataaagc tgaaaagcga aaagcagaca catggttaag aaatcaattt tttgcaactg
      541 aagaatacaa agaagcaaac gcaattgcgc tagttctttc ttttaatcat gaagtagata
      601 ctttttctat tattgaacaa gccttaatgg atcataaacg tatttttgtg ccgaaaatgg
      661 attatttaaa tcatcaaatg acttttaaag agatatttaa tctaaaagat attgatgtag
      721 ataagaaggg gatttactat ccaacttcaa aaggtgaaac aacgaataac ctagatttaa
      781 ttgttgttcc tggtgttgga tttcaagacg atggatatag aattgggtat ggtggtggct
      841 attatgacag gtttttagct aattatcaga caaagacaat aagcttatta tacgattttc
      901 aaataacatc atttgaaccg gaatcattcg atcaaccagt cgataaattg attatatatc
      961 aatcagcata gtggaggact aaatgaacat agacaaacaa ttttggaaaa caatatatta
     1021 ttggattagg tacttgaatt ttgatattgt cagtagggaa aaagatgacc aagaaatttg
     1081 gttagcccat aaaagaaaaa aacaagtagt catttttaaa caacatatca agtctaccca
     1141 agaaatacgt tttgacaaag cgaaagtgct tgaacacaaa gatgaaatag caaattttat
     1201 ttctttcgaa ccacaaagtt ttgaatttta ttattttaca gaatcggaat tttcagaaga
     1261 acaattaaat gaagtttcga caattagaat taaattcaat gttataagac acacaaaaga
     1321 tttgataaag catatgccga atatattttt ggctagactt atttcagaag ataatgataa
     1381 aaagacatat atgttctata aacgcaaagt attaaccgat aactttttag ataaatatat
     1441 gcagaaattt tcaccggcaa catacacaat aatatttgta aatgtcttaa tatggttatg
     1501 tatgatttta tatttaaata atttttcgga tgtaaaatta ttagatgttg gcgggttagt
     1561 gcattttaat gtcgtacatg gtgagtggta tcgaattgtt acatcgatgt ttttacattt
     1621 tagttttgaa catatactta tgaatatgct ttcattattt atttttggta aaatagtcga
     1681 agcaattatt ggttcatgga ggatgttaac tgtatacttt attgcagggc tgtttggaaa
     1741 ctttgtatca ctatcattta atacgactac aatttcagtt ggggctagtg gtgctatatt
     1801 cggtctgatc ggatcaattt ttgcgatgat gtatgtttca aaaacattta acaaaaaaat
     1861 gttaggacag ttattaattg cattagtgat attagttggt gtttctctgt ttatgtcaaa
     1921 tataaatatt gtggcgcata ttggaggctt cattggtggt ttattaataa ctttaattgg
     1981 ctattactat aaagtgaatc gtaatatttt ttggatttta ctaatcggta tgcttgttat
     2041 atttattgca cttcaaatta gaatttttac aattaaagaa gataatattt ataataaatt
     2101 gatcaaagat gatatgacta gtggtaatta tgataatgct caaaatattg tgaagcagac
     2161 aataaataaa aattatgccg atgatcaaac atattattta agtggtatga ttatggctac
     2221 tatcaattct aagtctgaag gtatgacaga atgggagcga ggacttagaa tgtttcctaa
     2281 atcaggttta ttaaattttg agttagcgat agcaaatcgt tcattaaatg atgatgaaaa
     2341 agcattaaaa tatgtgcgta aagcattaaa tgcagaccct aaaaatacag attatattaa
     2401 cttagaaaaa gagttgacta aatcaaatga gtcgaaaaat aaataacttt tatgatgtac
     2461 aacagttatt gaaaagttac ggatttctaa tatattttaa aaatccagaa gatatgtacg
     2521 aaatgattca acaggagatt tcatcattgt atcaatatga actgttatct aaggaagaat
     2581 atttgaaatg tacgttgata attaatcaga gaaggaatga acagaaatga caaaaattat
     2641 tttagcagct gatgtaggcg ggacgacttg taaattaggt attttcacac ctgaattaga
     2701 acaattacat aaatggtcta ttcacactga tacatctgat agtacaggtt atacactttt
     2761 gaaaggaatt tatgattcgt ttgttgaaaa agtaaatgaa aataattata atttttcaaa
     2821 tgtacttggc gtaggtattg gtgttccagg tcctgttgac tttgaaaaag gtacagtaaa
     2881 tggagcagta aacttatatt ggccagaaaa agttaatgta cgtgagattt ttgaacaatt
     2941 cgttgattgt ccagtgtatg tagataatga tgctaacata gctgctttag gggagaaaca
     3001 caaaggtgct ggtgaaggtg ccgatgatgt tgttgccatc acacttggta caggactagg
     3061 tggaggaatt atttcaaatg gtgaaatcgt acatggtcat aatggctctg gcgcagaaat
     3121 aggtcatttt agagcagact tcgatcaacg atttaaatgt aattgtggtc gttctggatg
     3181 tattgaaaca gttgcttcag cgacaggcgt tgttaactta gtcaacttct actatccgaa
     3241 gttgacgttt agatcttcta tattagaatt gattaaagaa aataaggtta cagcaaaagc
     3301 tgtttttgat gcggctaaag ctggtgacca attctgtatt ttcataactg aaaaggttgc
     3361 aaactatatt ggatatttat gtagtattat tagtgttaca agtaatccga aatatatcgt
     3421 tctaggtgga ggaatgtcta ctgcaggacc tattttaatt gaaaatatta aaacagaata
     3481 tcataattta acatttgcac ctgctcaatt tgaaactgaa attgtacaag cgaaattagg
     3541 taatgatgca ggtattacag gagcagcagg attaatcaag acctatgtat tagataaaga
     3601 gggggtaaaa taatggctat tgttgatgtg gttgttattc cagttggaac ggaaggtccg
     3661 agtgttagta aatatatagc agatattcag aaaaaacttc aagaatataa agcgatgggt
     3721 aaaattgatt ttcaattaac accaatgaat actctaattg aaggtgaatt aagcgatgta
     3781 ttagaagttg tgcaagtgat acatgaatta ccttttgata aaggtttaag tagagtttgt
     3841 acaaatatcc gtattgatga ccgacgagac aaatctagaa aaatgaatga taaactaaca
     3901 tcagtacaaa aacatttaga aaatagtggt gaaaacctat gaggatttca agcttaactt
     3961 taggcttagt tgatactaat acgtatttca tcgaaaatga caaagctgtt attctgattg
     4021 acccttcagg tgaaagtgaa aaaattatta aaaaattaaa ccaaataaat aaaccattaa
     4081 aagctatttt attaacacat gcacactttg atcatatcgg agcagtcgat gatatagttg
     4141 atcgattcga tgtcccggtt tatatgcatg aagcagagtt tgattttcta aaagatcccg
     4201 ttaaaaatgg agcagataaa tttaagcaat atggattacc aattattaca agtaaggtaa
     4261 ctcctgaaaa gttaaacgaa ggtagcacag aaatagaagg atttaagttt aatgtgttac
     4321 acacacctgg acattcacca ggaagtttaa catatgtgtt tgatgaattc gcagttgttg
     4381 gagatacatt atttaataat ggaatcggac gtacagattt atataaaggt gactatgaaa
     4441 cgctagttga ttctattcaa gataaaatat ttgaattaga aggcgattta cctttattcc
     4501 ctggacatgg tccatatacg acggttgatg atgaacaatt aaatccattt ttacacggtt
     4561 aaattgaagt aataaaaaca cctcctacgt gattatatgt aggaggtgtt tttttgaaga
     4621 ttctatttca agaaataatt aataaagcga tagaaatgaa agcgagtgat gtacatttta
     4681 ttccagttaa aaatgaagta agtattaaat ttagaattaa tgataacttg gagcagtatg
     4741 aacaaattgg gaatagcatt tatcaaaagt tattagttta tatgaagttt caagctgggc
     4801 ttgatgtttc tacacagcaa gtcgcacaga gcggtcgata tagttaccat ttcaataaaa
     4861 tatatttttt gagaatatca actttaccat tgtcacttgg ccaagaaagt tgtgttatca
     4921 gaattgtacc tcaatttttt caacaacaga aatcaactta taaattcaat gattttaaac
     4981 acctcatgaa taagaaacaa ggattactat tgtttagtgg gccaactggt tcaggaaaga
     5041 gtacattaat gtatcaaatg gtctcatacg cgaataaagc cttgaattta aatgtaattt
     5101 ctatagagga tcctgtagag atgcaaattc ctggtatcgt gcaaattaat gtgaatgata
     5161 aagctggcat taactatgta aattcgttta aagctatttt aagatgtgat cctgatgtta
     5221 ttttaatagg tgaaatcaga gataaagatg ttgccaagtg tgttatacag gctagtttaa
     5281 gtggtcacct tgttctgact acattgcatg caactgattg taaaggtgct attttaaggc
     5341 tattagaaat gggcatttct gtacaagaat tgatacaggc aactaactta attataaacc
     5401 aacgacttgt aactactatt aagcaacagc gacaattagt atgtgaaatt ctatctcagc
     5461 aacaactccg atatttcttt tcccataatc attcattacc atcatcattt aagaacttag
     5521 aagataaact tgatgatatg acaaaagcag gtgtcatttg tgaaactaca atggataaat
     5581 acatttaaac tacattctaa gaagcgacaa ttaagtaagg cccaacaaat cgatttactt
     5641 tcaaatttat gtaatttgtt gaaatatggt ttcactctgt atcaaagttt tcaattttta
     5701 aatcttcaaa tgacatataa aaataagcaa ttaggtacca ccattctaag tgaaatttca
     5761 aatggtgcac catgcaataa gatattatca ctgataggtt atagcgatac tatcgtcctg
     5821 caagtatatt tggcagaaag atttggcaat atcatagacg ttctagaaga aaccgtaaat
     5881 tatatgaaag tgaatagaaa gtcagaacaa cgattgttaa agacactgca atacccctta
     5941 atactagttt ctatttttat tgctatgatt attatattaa acctcacagt aattccacag
     6001 tttcaacaat tatatacttc tatgaatatt caactatctt cttttcaaaa aacattgtct
     6061 tttttcatta ccagcttacc tactataatt gtagtaatgc tcataatagt atctatgttg
     6121 gctattatta tgaaattaat ttataacaat ttaaatatgc tcaataagat aaactttgtg
     6181 atgaaactac cgctaatatc aggctatttc caattattta aaacttattt tgtaactaat
     6241 gaattagtgt tgttttataa aaatggtatt acacttcaat caatagtaga cgtttatatt
     6301 aaccatagta gtgatccatt tagacagttt ctaggtaaat acttattaac ttattcagaa
     6361 atgggatatg gtttgcctca aattttagaa aaactaaaat gctttaagcc tcaattaatt
     6421 aagtttgtgc tacaaggtga aaagagaggg aagctagaag tagaactaaa gttatattcg
     6481 caaatattag taaaacaaat agaagataaa gcgataaaac agactcagtt tttacagcct
     6541 attttatttt tgattttagg tttatttatt gtcgcaattt atttagtaat tatgttaccg
     6601 atgtttcaaa tgatgcaaag tataaaataa tggaggttaa tatatgttta aatttcttaa
     6661 gaaaactcaa gcgtttacat tgatagagat gctattagtg ttattaatca tcagtttatt
     6721 attaatttta atcattccaa atattgctaa acaaactgct cacatacaat caacaggttg
     6781 taatgcacag gtaaaaatgg ttaatagtca aattgaagcg tatgcattga aacataatag
     6841 aaatccatcg tctattgaag acttaattgc agatggtttt ataaaagaag agcaaaagac
     6901 atgtaaatca ggagagacaa taacaattag taatggagaa gcagttgcaa attagaaaac
     6961 agtcagcatt tactatgatt gagatgcttg tggtaatgat gttaatcagt atatttttac
     7021 ttttgacatt gacatctaaa ggattaagca atcttagagt aatagatgat gaggcaaata
     7081 tcatttctct tattactgaa ttgaattata ttaagtcgca agctatagca aatcaaggat
     7141 atatcaatgt tagattttat gaaaacagtg acactattaa agtaatagag aataataaaa
     7201 tacgatttct aaaactaaaa gtaggcaaaa taattaatgt tgcaaaagtt gatattattg
     7261 cctttgataa aaaagggaat atcaataaat ttggtagcat aacaattgat aataacaatt
     7321 caatttatag aataatattc catattgaaa aaggaagaat tcgttatgaa aagctataag
     7381 tgtaaaggat cattcttaat agatagtatg gctggatttt tgcttattgg attgattaca
     7441 ttactattga taccaatgat gaatcaaatg caagcgagta taaaccataa actacaaaca
     7501 attgatgctt ctaaagtaat tttgacgact gtatctaaaa ttaataaaga agaacttaag
     7561 aagggggtaa ctatagggaa atatgatatt aagcaaagtg accaacaaat ttgtgctatt
     7621 tcaaaaaata ccacttctta tcaaaagaca tgtatacagt attaatgtca aagctttttc
     7681 gctaattgaa atgttagtag cgatgatggt tataagtata actttactaa ttgttccaga
     7741 cttaattaga cttaacaaaa cttttctaat tgaaagtagg gaattaacaa ctgtagattt
     7801 cgaatttttc tcaagagata ttctagagga ttttaaaggt gtagatagaa acgatattga
     7861 aattaggcaa cagcgtatca ttttacataa aggtgaagaa atgatcgaat acaaattaat
     7921 aaataataaa attattaaag ttgtaaatga cagaggaaat ataacaatga ttaataatgt
     7981 tacagcattt actgcaaata tctactataa atctattatt aaaataacga taacagttaa
     8041 agtcggtaca aatctgcaga ctaaaacaat ttatgtataa cttactagaa tttaaatgag
     8101 tactattaaa aaggacgtga aataatttat taattttgat ttatataaag aaaaaggata
     8161 tgttacgccg ttcttattga tgctaacagc aatttatttt tccattttca ctttttatat
     8221 cagtcaatat agtttaaaat taaagacatt atataatatt gaaactgact ataactataa
     8281 gattgttttg ttattgaaag aagggaaaac gcatgaatca tgataaatca ccaataattt
     8341 taattggttt catgggtacc ggtaaatcta cgattggtaa atacgttgca gatgagcaaa
     8401 atttatcatt tattgatata gattcatata tcgaagaaaa gtataagtta acaataccag
     8461 aaatatttag taaacatggt gaacaatatt tcaggaattt agagttcaca tgtttgcaag
     8521 aatgtattaa cactgcagat ataattgcta ctggtggtgg tattattgaa agtgaagagg
     8581 tatttaattt tttgaaaaat caaaaaaaca ttatttggtt agattgtaat attgatatta
     8641 tatatagtcg aatcaatgat gacccacatc gacctaatgc aaataataag acaatcaagc
     8701 agttaaatga cttgtattgc tcgcggattt taagatataa tgaaatcgca ttcaagaaat
     8761 ttgatagtca tttgctatca atttcagaaa tatattatga attgctaaat ttaataaaag
     8821 cgagtgatca gtattagaga gaatagagcg ttaagactct atcgccgaag gtgcaagtaa
     8881 tttattacga aactctcagg caaaaggata atactgtaac gcgttcctga attggtgatt
     8941 tataaacagg gtagcgattg ctatcctgtt tttataattt taagggggta tttcaatgtc
     9001 aagtgattta aaacaaacac ctttatatca aaattatgtt gatagaggtg caaaaattgt
     9061 ggaattcgga ggatgggcga tgcctgttca attttcaagt attaaagagg agcataatgc
     9121 tgttcgatac gaaattggcc tgtttgatgt tagtcatatg ggtgaaattg aagtgacagg
     9181 taaagatgct agtcagtttg tgcaatattt attatcaaat gatactgata atttaactac
     9241 ttcaaaagca ttatatactg ctttatgtaa tgaagaaggc ggtattattg atgatttagt
     9301 aatatataaa ttagctgacg acaattattt attagttgtt aatgctgcta atactgaaaa
     9361 agattttaat tggattttaa aacacaaaga gaaatttgat gttgaagtac aaaatgtatc
     9421 aaaccaatat ggtcaattag caatacaagg accaaaagct agagatttaa ttaatcaatt
     9481 agttgatgaa gatgtaactg aaatgaaaat gtttgaattt aaacagggtg tcaaattatt
     9541 tggagcgatc gtcattttat ctcagtcagg ttacacaggt gaagatgggt ttgaaattta
     9601 ttgtaatatc gatgatactg aaaaaatttg ggatggttta ttagagtata atgttatgcc
     9661 atgtgggtta ggcgctcgtg atacattaag attagaggct ggattgccat tacatggaca
     9721 agatttaact gaatcaatta caccatatga aggtggtatc gcttttgcaa gtaaaccatt
     9781 aattgatgct gattttattg gtaaatctgt attaaaagat caaaaggaaa atggtgcacc
     9841 aagaagaaca gtgggattag aattacttga aaaaggaatt gcaagaactg gttatgaagt
     9901 tatggattta gatggaaata ttattggaga agtaacttca ggaacacagt ctccatcatc
     9961 aggaaaatca attgcacttg caatgataaa aagagatgag tttgaaatgg gtagagagtt
    10021 gcttgttcaa gttcgtaagc gtcaattaaa agcgaaaatt gttaagaaaa atcaaattga
    10081 taaataatta aaaaggggtg tgcattgtga gtcatcgtta tataccttta actgaaaaag
    10141 acaagcaaga aatgttacaa acaattggtg caaaatctat aggagaatta ttcggtgatg
    10201 taccaagtga cattttatta aatagagatt taaatattgc tgaaagcgaa gcggaaacaa
    10261 cgttacttag aagattaaat cgcattgcaa gcaagaatat cactaaagaa acgcatacat
    10321 catttttggg tgctggtgtt tatgatcatt acgcgccatc agttgtggat gctatgattt
    10381 caagatcaga attttacaca gcatatacgc catatcaacc ggaaatttct caaggtgaat
    10441 tacaagccat ttttgagttc caaactttaa tttgtgaatt aacagatatg gatgtagcaa
    10501 attcttctat gtatgatggg atgactagct ttgctgaagc atgtatttta gcatttagtc
    10561 aaacaaagaa aaataaaatt gtagtctcaa aaggtttgca ttatcaagca ttacaagtac
    10621 ttcatactta tgctaagacg cgtaaggaat tcgaagtagt ggaaatcgat ttagatggca
    10681 ctgttacaga tttgaaaaaa ttagaagcag cagtggatga tgatacagca gctgttgcag
    10741 ttcaatatcc aaacttctat ggttcaattg aagatcttga aaagattcat agctttattg
    10801 aagataaaaa agcattattt atcgtatatg caaatccatt agcattggga ttacttacac
    10861 ctccaggttc atttggtgca gatattgttg taggtgatac tcagccattt ggaattcctg
    10921 ctcaattcgg tgggccacat tgtggatatt ttgcaacgac gaaaaagtta atgcgtaaag
    10981 taccaggtag attagtcggt caaacacaag atgatgaagg taatcgtggc tttgtattaa
    11041 cattacaagc acgtgaacaa catattcgac gcgataaagc gacatctaat atttgttcta
    11101 accaagcatt aaatgcccta gcgtcatcaa ttgcaatgtc agcacttggt aaacaaggta
    11161 tctatgatat tgcagttcaa aatatagaac atgctaacta tgctaaacaa caatttatta
    11221 aaaaaggttt tgaagtatta gatggtacat ctttcaatga gtttgtagtc aaatttgaca
    11281 aaccaattca acaagttaac gaggaattag tgaagtacaa tattatcggt ggttttgatt
    11341 taggtgttgt ttcagacgac tttaaaaatc acatgttaat agctgtaact gaattaagaa
    11401 caaaagatga aatcgataca tttgtagaaa aggctggtga gttaaatgac tagtaaatca
    11461 agtccattaa tttttgaaag atctcgtgaa ggtagatatg catattcatt accaataagt
    11521 gatattaaaa caaattctgt tgagtcattg ttagatgata aatttattcg taaaaacaaa
    11581 gcggagtttc ctgaagttgc tgaactagac ttagtacgtc attatactga actttcaaat
    11641 aaaaatttcg gtgtagataa cggattttat ccattaggtt catgtacgat gaagtataac
    11701 cctaaaatta atgaaaaggt tgctagaata ccaggattca gtgaatcaca tccattacaa
    11761 gatgaagacc aagttcaagg ttcattagaa attatttata gtttacaaga agaattaaaa
    11821 gaaattactg gtatggatga ggtaacatta caaccagctg ctggtgcaca tggtgaatgg
    11881 actgcattga tgatatttaa agcttatcat gagaataatg gtgaaggtca tcgtgatgaa
    11941 gtcattgtgc cagattctgc gcatggtacg aatccagcct cagcttcatt tgcaggattt
    12001 aaatcagtta ctgtaaaatc aaacgaacgt ggcgaagttg atattgatga cttgaaacgt
    12061 gttgtaaatg aaaatacagc agctattatg ttaactaatc caaacacttt aggtattttc
    12121 gaaaaaaata ttatggaaat ccgtgaaatc gtccataatg ctggtggtct attatattat
    12181 gatggtgcga atttaaacgc tattatggac aaagttcgcc caggagatat gggatttgat
    12241 gctgttcatt taaacttgca taaaacattt actggtccac atggtggcgg cggtcctggt
    12301 tcaggtccag tcggtgtagt aaaagaacta gcaagttact taccaaagcc aatggttatt
    12361 aaagatggcg acaaatttaa atatgataat gacattaaaa attctatcgg acgtgtaaaa
    12421 ccattttatg gtaactttgg tatttactta agagcttata cgtatattcg aactatggga
    12481 gcaactggcc ttaaagaggt ttccgaagca gcggttctta atgcgaatta tattaaagca
    12541 cgtttatctg aacactttga aataccttat aaacaatatt gtaaacacga gtttgtgtta
    12601 agtggtgttc gtcaaaaaga atttggtgta cgtactttag acatggctaa gcgattatta
    12661 gatttcggtg tacatccacc aacaatctac tttccattaa atgttgaaga aggtatgatg
    12721 attgaaccga ctgagacaga gtctaaagaa acacttgatt attttatcga tacattaatt
    12781 agtattgctg aagaagctaa aaatgatcct gataaagtgt tagaagcacc atatacaact
    12841 gtgattgatc gattagacga agctacagct gctcgtaaac caatattaaa gtttgaaaat
    12901 cttaaacagg aaaaataagg tgttaaacac atattccgag aattattatt ctaactttgt
    12961 atgaagattt aaggataatg gttttaaaat caattaaaaa agacaatttc tatttgaaaa
    13021 agaaaactaa accgaagtaa taactcttag ggtttggtat tattctttca tagaaattgt
    13081 cttttcactt tttagattga ggtaattgaa tcgtattgaa aatgagttga actctcttta
    13141 ttatgcagaa actaaattta atgatctgtg aagctagaga aatatagtaa cagtctttgt
    13201 taaaaaatta aaatctgagt attgttattg atgatcaatt aacactcaga ttttattaaa
    13261 tcgtatatta aatttacaaa actatttttt agactttatt tttccagtcc attttttata
    13321 gccgcctttt aacatataga tatctgtata tccattcttt ttcaaaatac gagcggctct
    13381 atagctagca atcccattgg catcacataa gtataccggt tggtcttttc ttaatccttg
    13441 gaatcgttgc ctgaacattg tcataggaat attgcgagac ccattaatgt gaccgtagtc
    13501 atagtcaact ttctctctaa catcgatgac ttgagctttt ctaatcccat tatggaattc
    13561 attttgatct aattctttaa cagctcgctt gttaagaatt tgttgaacaa tcatataagc
    13621 aataattgct ataactaaaa ttattgcgat gtacaaacta gcactcatct tgcatcctcc
    13681 ctaattaaac gataatataa ttataagact gttaggtcaa attatcaaaa tatttcacta
    13741 aagaattgca attattattc atttagttaa attgattaat tagcgaatca aatattatac
    13801 attttgctga taggagttct tgaatatgac tgaaacttgg aattttatta atacaggaag
    13861 taaagaccca tattataata tggcaatgga tgaagcgtta ttaaattttg tctctagagg
    13921 tgaaattgat ccagttatac gtttttacac atggaatcct gcaacattat caataggtta
    13981 tttccaaagg ctacaaaaag aaattgatat tgataaggtt aaagagaaag gttttggtct
    14041 cgtaaggcgt caaactggtg gccgtggggt gcttcatgat aaagagctaa cttacagtgt
    14101 gatagtgcct gaatcgcatc ccaatatgcc ttctactgtt acagaagcgt acagagtgat
    14161 ttcacaagga ttattagaag gatttaaaaa cttaggattt gatacttatt ttgcggtacc
    14221 taaaacgcct gaagagcgtc aaaaattaaa acaacctcga agttcagtgt gttttgatgc
    14281 accaagttgg tatgagctag ttgttgaagg ccgtaagatt gctgggagtg ctcaaacgag
    14341 acaaaagggc gtaatcctcc aacacggttc gattttacaa gatattgata ttgacgaatt
    14401 gtttgatatg tttatttata aaaatgaaag attaaaatta aaaatgaaag aggcattcgt
    14461 tgaaaaagca gttgccatca atgatatttc agatgaacac attactatta gtcaaatgga
    14521 agaagctttc gagaaaggtt ttaagaaagg tttaaatata gaattaaagc cacttgaatt
    14581 aacagaagtt caattggcag aagtggaaga attaactgaa aaatatcgtt cagatgaatg
    14641 gatgtttaga aagtagtttt tagaataaaa aatggaagca cgtagactat tttaactaca
    14701 agtgcttcca tttttttatc tattttcttc tttgatttct tttatactta cgcattgctt
    14761 tgcgatattt gcgttggtct tcagttaaga tgaagaaata ataaatcaga taaataatac
    14821 cagctattac agcaaagctt actaacattc tgacgattga aaaaataaag gcatctagat
    14881 tcattactaa tccaatcgca gcaattataa gtacaaggta aaatattata ttacgcatta
    14941 aaagactcct atcatgattt tgcatttaag tttaattttg acaacgcttg ttgtcctttt
    15001 gtaagttgtt ttttatcttt attttgatag ccatttttaa tatcatcaaa tgcatgattc
    15061 aaatcagagt ttaatttatc gatattttta gacgtttttt tatcatcatc actaagtgat
    15121 aaaccatcga tagcatcttt gtatgaagtg tgagcacttt gaatatcatt agaaatttga
    15181 tttaatttcg attgaacatt cttatcgcct ttgtttttac ttacatcctt ttcaatatcg
    15241 ttataggatt tcaaagcttt agcaactttg tcgtagtatg ttgatgtaac attcatcaat
    15301 ttaattttcg tattattaga tgcagttttt ttactatttt ctttatcctt ttcaagccct
    15361 ttaattttag atttagaatc actaatttgt tgattaagtt cttgaatatc tagtttgagt
    15421 tgatgattat cttctcttaa atctgttgtt ttttcttcta atggtgctaa attttgtgat
    15481 ccacatccag ctaacaataa tgttgcgcca acaattgaaa ccaatttttt cattactatc
    15541 tccctaaaat caatatctat tattttttat ccttattatg atattatttt aatgtataaa
    15601 gttgaaatgc acaaacgttt cactaggagg taataagatg agcagaataa cacaagtgca
    15661 tcgtatttta gaacaaaaac atttagatgc gattatcatt ctttcggatt acaatagaag
    15721 atatttatca ggttttactg gtaccagtgg agcacttatc atatctaaag ataaacaata
    15781 tttgataaca gactttagat atattgatca agcaactaag caggcgccaa attatgaaat
    15841 tattaatcgt aaatctacta tcattggtga gattaaagaa ttgctacacc aagagaattt
    15901 tgaaaatgta ggctttgagg ggcaccatgt gagttatgat acataccttg aattaaataa
    15961 aagccgtata tcattaataa gcatttctaa tactgtagat aaaattagag acgtaaaaga
    16021 tgctgacgaa attgcattga ttcaaaaagc agcagatatc gttgatgaaa catatgaata
    16081 catactaacg gttgcaaaag ctggtatgac tgaaaaagaa ttaaaggcaa tattagaaag
    16141 taaaatgcta gaattaggag cagatggacc atcattcgat acgattgtag catctggtca
    16201 tagaggtgca ttaccacatg gtgttgcaag tgacaaaatt attgaaaaag gcgacatgat
    16261 aacattggat tttggtgctt atttcaatgg atattgttca gatattacta gaacatttgc
    16321 tattggagaa ccagatccta aactgaaaga aatatatcaa atagtacttg aatctcaaat
    16381 gaaagcaatt aatgagatta gacctggcat gactggtgca gaagctgatg ccatttcaag
    16441 aaactattta gagtcaaaag ggtatggaaa agaatttgga cattcactag gacatggtat
    16501 tggtttagaa atccatgaag ggccaatgct ggctcgtacg atacaagata aacttcaagt
    16561 taacaactgt gttacagtag aacctggtgt ttatatagaa ggtttgggcg gtataagaat
    16621 agaagatgat attttaatta cagaaaatgg ttgtcaagtc tttactaaat gcacaaaaga
    16681 ccttatagtt ttaacataag cgtgtaaaat gaggaggaaa ctgaatgatt tcggttaatg
    16741 attttaaaac aggtttaaca atttctgttg ataacgctat ttggaaagtt atagacttcc
    16801 aacatgtaaa gcctggtaaa ggttcagcat tcgttcgttc aaaattacgt aatttaagaa
    16861 ctggtgcaat tcaagagaaa acgtttagag ctggtgaaaa agttgaacca gcaatgattg
    16921 aaaatcgtcg catgcaatat ttatatgctg acggagataa tcatgtattt atggataatg
    16981 aaagctttga acaaacagaa ctttcaagtg attacttaaa agaagaattg aattacttaa
    17041 aagaaggtat ggaagtacaa attcaaacat acgaaggtga aactatcggt gttgaattac
    17101 ctaaaactgt tgaattaaca gtaactgaaa cagaacctgg tattaaaggt gatactgcaa
    17161 ccggtgctac taaatcggca actgttgaaa caggttatac attaaatgta cctttatttg
    17221 taaacgaagg tgacgtttta attatcaaca ctggtgatgg aagctacatt tcaagaggat
    17281 aatctctaat ttgttaacaa atagcttgta ttcactatac tgatttaacg taagatattc
    17341 caaataagtc tcataaagct attgcctaaa atgattatag gttatatgct gatatgaggc
    17401 tttttatttt taaaataaat tttaaaatat aaaacgaatc gtctaattgg tagtaaaaaa
    17461 acatagacat gagtaagggt atatatctac atatcatatt gttgaggata agaaataata
    17521 gctaataaaa ataacattga tatgtttaac tgaatgcata aaatttttaa tcaagagtta
    17581 agatgagtca atgttaagta ataaatccac aagtgttttt aatttgaaat gtggttttat
    17641 tataactttg ggatagacct ataatgtcgc ctgcgatatg aacatgcttg aattgaagag
    17701 ttgtctcaag taaaatagac gggtagatga aaacaaactg aaggagtcag taataatgaa
    17761 ctttaaagaa atcaaagaat taattgaaat tctggataaa tcaactttaa cggaaatcaa
    17821 tattgaagat actaaaggca aagtgacgct taagaaagaa aaagaaactg agattatcac
    17881 gccacaaatc tcacaaatgc catttgaagc tgcggcaatg cctatgcctc aagtacaatc
    17941 aactgataac aataaaactg aagctccaaa gccaacttca gataatcaca aaacaattaa
    18001 tgcacctatg gtaggtacat tttacaaatc gccatctcca gacgaagaag cctatgtgca
    18061 agttggggac actgtttcaa atgaaacaac agtgtgtatt ttagaagcaa tgaaactatt
    18121 taatgaaatt caagcagaaa tttcaggtga aattgttgag atcttagtag aagacggaca
    18181 aatggtagag tatggccaac cgttatttaa ggtgaaataa tgaaaaaggt tttaattgca
    18241 aaccgcggtg aaatcgcagt taggattatt cgcgcttgtc gtgatttagg catccaaact
    18301 gttgcaatct attctgaagg ggataaagat gcgctacata ctcaaattgc tgatgaagca
    18361 tattgcgtag gccccacttt gtctaaagat tcatatttaa atattccgaa catcttatct
    18421 attgcaactt ctacaggttg tgatggcgtt catccgggtt atggcttttt agctgaaaat
    18481 gctgactttg cagaattatg cgaagcatgc caattgaagt tcattggacc aagttatcaa
    18541 tctatccaaa aaatgggtat caaagatgtt gctaaggcag aaatgatcaa agccaatgtt
    18601 ccagttgttc ctggtagtga cggtttaatg aaagacgtct cagaagctaa gaaaatcgct
    18661 aaaaaaattg gttatccggt catcattaaa gctactgctg gtggtggcgg aaaaggtatc
    18721 cgtgttgctc gtgatgaaaa agaacttgaa actggcttcc gaatgacaga acaagaagca
    18781 caaactgcat ttggtaatgg tggactttat atggagaaat tcatcgaaaa cttccgccat
    18841 attgaaatcc aaattgttgg ggacagctat ggtaatgtaa ttcatttagg agaacgtgat
    18901 tgtacaattc aaagacgtat gcagaaatta gtggaagaag caccttcccc aattttagat
    18961 gatgaaacac gtcgtgaaat gggaaatgca gcagttcgtg cagcgaaagc tgtaaattat
    19021 gaaaatgcgg gaacaattga gtttatatat gatttaaatg ataataaatt ttattttatg
    19081 gaaatgaata cacgcattca agtagaacat cctgtaactg aaatggtaac aggaattgat
    19141 ttagttaaat tacaattaca agttgctatg ggtgacgtgt taccgtataa acaagaagat
    19201 attaaattaa caggacacgc aattgaattt agaattaatg ctgaaaatcc ttacaagaac
    19261 tttatgccat caccaggtaa aattgagcaa tatcttgcac caggtggata tggtgttcga
    19321 atagagtcag catgttatac taattatacg ataccgccat attatgattc gatggtagcg
    19381 aaattaatca tacatgaacc gacacgagat gaagcgatta tggctggcat tcgtgcatta
    19441 agtgaatttg tggttcttgg tattgataca actattccat tccatattaa attattgaat
    19501 aacgatatat ttagaagcgg taaatttaat acaaactttt tagagcaaaa tagcattatg
    19561 aatgatgaag gttaatagga ggtcaatcca tggtcaaagt aactgattat tcaaattcaa
    19621 aattaggtaa agtagaaata gcgccagaag tgctatctgt tattgcaagt atagctactt
    19681 cggaagtcga aggcatcact ggccattttg ctgaattaaa agaaacaaat ttagaaaaag
    19741 ttagtcgtaa aaatttaagc cgtgatttaa aaatcgagag taaagaagat ggcatatata
    19801 tagatgtata ttgtgcatta aaacatggtg ttaatatttc aaaaactgca aacaaaattc
    19861 aaacgtcaat ttttaattca atttctaata tgacagcgat agaacctaag caaattaata
    19921 ttcacattac acaaatcgtt attgaaaagt aatgtcatac ctaattcagt aattaaataa
    19981 agaaaaatac aaacgtttga aggagttaaa aatgagtcgt aaagaatccc gagtgcaagc
    20041 ttttcaaact ttatttcaat tagaaatgaa ggacagtgat ttaacgataa atgaagcgat
    20101 aagctttatt aaagacgata atccagattt agacttcgaa tttattcatt ggctagtttc
    20161 tggcgttaaa gatcacgaac ctgtattaga cgagacaatt agtcctaatt taaaagattg
    20221 gactattgca cgtttattaa aaacggatcg tattatttta agaatggcaa catatgaaat
    20281 attacacagt gatacacctg ctaaagtcgt aatgaatgaa gcagttgaat taacaaaaca
    20341 attcagtgat gatgatcatt ataaatttat aaatggtgta ttgagtaata taaaaaaata
    20401 aaattgagtg atgttatatg tcagattatt taagtgtttc agctttaacg aaatatatta
    20461 aatataaatt tgatcaagac ccacatctcc aatctgtatt gattaaaggt gagctttcaa
    20521 atttcaaaaa gcattcaagt ggtcatttat actttaatgt gaaagataaa gaaagcgtta
    20581 taagtgccat gatgttcaaa ggtagtgctt ctaaattaaa cttcgaacct aaagaaggag
    20641 atgaagtctt attagaagca cgtgtttctg tttttgaacg tcgtggaaac tatcaaattt
    20701 atgtaaataa aatgcaatta gatggtatag ggaacttata tcaaaaatta gaagcattaa
    20761 agaaaaaatt aactgaagaa ggttgttttg ataaagcgaa taagaaatca atacctaagt
    20821 ttcctaaaaa aattgcagtt ttaacagcga gtacaggtgc cgcaattcga gatatccatt
    20881 caacgataaa tagtcgtttt ccattagctg aacaaataca aattagtact ttggttcaag
    20941 gtgaaaaagc aaaagacgac attattgaaa aaattgaata tgcagatagt ttaggtgtag
    21001 ataccattat tgtaggtcga ggcggtggat ccatagaaga tttatggaat ttcaacgaag
    21061 aagctgtcgt ccgtgcaata tataattgta agacaccgat tatatcagca gtaggtcatg
    21121 aaacagactt tacattaagt gattttgctg cagacatcag agctgcgact ccaactcaag
    21181 ctgctgttat tgcaacacca gaccaatatg aattgctgca acaaatacag caatatcagt
    21241 ttacattgac tcgtttcatc aagaaacatt tagaacaaca acgtaaacat gttgaacatt
    21301 tgtcatcgta ttacaagttt aaacaaccaa ctttattgta tgatcaacag atacaacgtc
    21361 gtgatgattt agaaaagaga ctgaaacaac aaattcaggc aacttttgaa caacagagac
    21421 atcgattaat gctgttgcag caacgctata atttaaaagc gttactaagt agtgttaatc
    21481 aagaacaaca aaataatctt caattgacaa accaacttgt taaattattg aattctaaga
    21541 tattaagtta taaaaatgat ttgaaaaata aagtagaaaa cttaaacaat ttaagtccaa
    21601 ctaatacaat gttgcgtgga tatgcaattg ttaataaaaa agacgaagtc attacgagta
    21661 ctaaagattt aacagaaaat gatcaattga cgttaacaat gaaagatggc ttagtagatg
    21721 caaaagttac gaaagtaagg tgtaataatg actaaagaaa cgcaaagttt tgaagaaatg
    21781 atgcaagaat tagagcgaat tgttcaaaaa ttagataatg aaacagtatc tttagaggaa
    21841 tcattagatt tataccaacg tggtatgaaa ctatcagcag cttgtgacac aactttaaaa
    21901 aatgccgaaa aaaaggtgaa tgacttaata aaagaagaag ctgaggatgt aaaaaatgac
    21961 gaatctaccg atgaataaat taatagatga agtcaataat gaattatcgg ttgcgataaa
    22021 taaatcagta atgaatactc agctagaaga aagtatgttg tattcattaa atgctggagg
    22081 taaacgcatc cgaccagttc tgttattact cactttagat tcactaaata ccgagtatga
    22141 gttaggtatg aagagcgcaa ttgcactaga aatgattcat acatattcac ttattcatga
    22201 tgacctacca gcgatggata atgatgatta tcgacgaggg aaattaacga atcataaagt
    22261 atatggtgag tggacggcga tattagcagg tgatgcttta ttaactaaag catttgaact
    22321 tatttcaagt gatgatagat taactgatga agtaaaaata aaagttctac aacggctgtc
    22381 aatagcaagt ggtcatgttg gaatggtcgg cggtcaaatg ttagatatgc aaagcgaagg
    22441 ccaaccaatt gatcttgaaa ctttggaaat gatacacaaa acaaaaacag gagcactatt
    22501 aacttttgcg gttatgagtg cagcagatat cgctaatgtc gatgatgcaa ctaaagaaca
    22561 tttagaaagt tatagttatc atttaggtat gatgtttcag attaaagatg atttattaga
    22621 ctgctatggt gatgaagcga agttaggtaa aaaagtgggc agcgatcttg aaaataataa
    22681 aagtacgtac gtgagtttat taggaaaaga tggcgcagaa gataaattga cttatcatag
    22741 agacgcagca gtggatgaac taatgcaaat tgatgaacaa ttcaatacaa aacacttatt
    22801 agaaatcgtt gatttatttt atagcaggga tcactaaaag caaatatatc gattagcaac
    22861 aattgttaag tttgaaatgt ctcaaaaggc atattcaact gacgttgttt gttaatcgat
    22921 ttttctcatg ttgaaactta aaattatgtt aaagactttc atctaaaaat agaattttca
    22981 atttctatac gacaatattc gcatctgtga tatcaatgtg attttaatta aatatgttca
    23041 aacaattgac gcttattaaa tctaataatt agcaagttta ttacacatat attcattttt
    23101 atacatattt taagcaattg ttatgcgtaa tatgaaaaat atttataaat agataatcaa
    23161 gatagataaa ccatgtttac cctttttacg taaaggtgtt tagtagtcgt aaatcagtgc
    23221 atgtgttaaa ataaatgtat aaatattcgt aatcagaggt gtttactgtg cccaaaaaat
    23281 cggttaggca tataaaaatt agagaaatta tttcaaatga acagatagag acacaagatg
    23341 aattagttaa acgattaaac gattatgatt taaatgtcac tcaagcaact gtttctcgtg
    23401 atattaaaga actacaactt attaaagtac ctataccttc aggtcaatat gtttatagtt
    23461 taccaaatga tagaaaattc catcctttag aaaaattggg acgttattta atggattcct
    23521 ttgttaatat agatggtact gataatttac ttgttctaaa aacattacct ggtaatgcac
    23581 aatctattgg agctatatta gatcaaatca attgggaaga agtactaggc acaatttgtg
    23641 gtgatgatac ttgtttaatt atttgtcgaa gcaaagaggc aagtgatgaa atcaagtcaa
    23701 gaattttcaa tttgttataa ggatgcgatg agtttatgtt acaaacctta tcaatcaagc
    23761 aatttgctat tattgaagag ttagaaattc aattttctga tggtttaact gttttaagtg
    23821 gtgaaacggg ttcaggtaag tcaattatta tagatgcaat tggacaatta attggaatga
    23881 gagcttcatc tgattttgta agacatggtg aaaaaaaggc tgtcatcgaa ggtatatttg
    23941 atattgacga gagtaaagat gctatacata tattgaaaaa tatggatatt gatgtagatg
    24001 aagatttttt
//
LOCUS       DAMZPP010000024        23558 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMZPP010000024 DAMZPP010000000
VERSION     DAMZPP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23558
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.024"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..446)
                     /locus_tag="POH73_000747"
     CDS             complement(<1..446)
                     /locus_tag="POH73_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDI6886722.1"
                     /translation="MKDLTMLLHELKDMSFFNKGDICLIGCPTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQFNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSV"
     gene            complement(553..972)
                     /locus_tag="POH73_000748"
     CDS             complement(553..972)
                     /locus_tag="POH73_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDI6886723.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            complement(969..2015)
                     /locus_tag="POH73_000749"
     CDS             complement(969..2015)
                     /locus_tag="POH73_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDI6886724.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKPGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            complement(2099..2935)
                     /gene="prmC"
                     /locus_tag="POH73_000750"
     CDS             complement(2099..2935)
                     /gene="prmC"
                     /locus_tag="POH73_000750"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDI6886725.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLIKEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            complement(2922..3998)
                     /gene="prfA"
                     /locus_tag="POH73_000751"
     CDS             complement(2922..3998)
                     /gene="prfA"
                     /locus_tag="POH73_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDI6886726.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESSGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            complement(3999..4598)
                     /locus_tag="POH73_000752"
     CDS             complement(3999..4598)
                     /locus_tag="POH73_000752"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDI6886727.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            complement(4945..5199)
                     /locus_tag="POH73_000753"
     CDS             complement(4945..5199)
                     /locus_tag="POH73_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDI6886728.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            complement(5317..6633)
                     /gene="rho"
                     /locus_tag="POH73_000754"
     CDS             complement(5317..6633)
                     /gene="rho"
                     /locus_tag="POH73_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDI6886729.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            complement(6880..8307)
                     /locus_tag="POH73_000755"
     CDS             complement(6880..8307)
                     /locus_tag="POH73_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDI6886730.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEAIGKVAKGNKADV
                     DKAVEAADNVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            complement(8545..8880)
                     /locus_tag="POH73_000756"
     CDS             complement(8545..8880)
                     /locus_tag="POH73_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6886731.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            complement(8969..10228)
                     /locus_tag="POH73_000757"
     CDS             complement(8969..10228)
                     /locus_tag="POH73_000757"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDI6886732.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     VLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYRFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            complement(10694..11554)
                     /locus_tag="POH73_000758"
     CDS             complement(10694..11554)
                     /locus_tag="POH73_000758"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDI6886733.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTESQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            11772..12293
                     /locus_tag="POH73_000759"
     CDS             11772..12293
                     /locus_tag="POH73_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HDI6886734.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     ALDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            complement(12402..14012)
                     /locus_tag="POH73_000760"
     CDS             complement(12402..14012)
                     /locus_tag="POH73_000760"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDI6886735.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGASPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(14348..14878)
                     /locus_tag="POH73_000761"
     CDS             complement(14348..14878)
                     /locus_tag="POH73_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDI6886736.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(14990..15850)
                     /locus_tag="POH73_000762"
     CDS             complement(14990..15850)
                     /locus_tag="POH73_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6886737.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            16274..17077
                     /gene="coaW"
                     /locus_tag="POH73_000763"
     CDS             16274..17077
                     /gene="coaW"
                     /locus_tag="POH73_000763"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HDI6886738.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLGK"
     gene            complement(17203..17874)
                     /locus_tag="POH73_000764"
     CDS             complement(17203..17874)
                     /locus_tag="POH73_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HDI6886739.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(18273..19466)
                     /locus_tag="POH73_000765"
     CDS             complement(18273..19466)
                     /locus_tag="POH73_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDI6886740.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPFVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKQATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            complement(19466..20650)
                     /locus_tag="POH73_000766"
     CDS             complement(19466..20650)
                     /locus_tag="POH73_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDI6886741.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSDLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIKTGCDYEFGP
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            20960..21430
                     /locus_tag="POH73_000767"
     CDS             20960..21430
                     /locus_tag="POH73_000767"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDI6886742.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            complement(21582..21932)
                     /locus_tag="POH73_000768"
     CDS             complement(21582..21932)
                     /locus_tag="POH73_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886743.1"
                     /translation="MNILRYLLGIGFSIIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(21946..23247)
                     /locus_tag="POH73_000769"
     CDS             complement(21946..23247)
                     /locus_tag="POH73_000769"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDI6886744.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIDGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAIDSGAALEKFKTFIKNQGGDKTVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADYVVSPTLIHKIITE"
BASE COUNT         7265 a         4609 c         3298 g         8386 t
ORIGIN      
        1 tttactgaag tccttacagg tacacataca tgtttaatat gcatgcctat taacgtttga
       61 ccaatatcaa ttccacaagg aacagtaata tgttcgacca cgattggatc cttcatatgc
      121 tgaaaagcgt atgttgccaa actccctcca gcatgtacat ctggaacgac ggaaacttct
      181 tccattgtta atggattaaa ctgagatttt tctattgtta tcgctctgtt gatatgttca
      241 catccttgaa aagcaaaagt aacgcctgtc tctttactca caacatctaa tgcattaaaa
      301 atagtttctg caacttccat cgaaccgaca gtccctattt tttcgccaat gacttccgat
      361 gttggacatc caattaaaca tatatctcct ttattaaaaa aggacatatc ttttaattcg
      421 tgtaataaca ttgtcaaatc tttcataaaa gcccaccctt cctaaaaata aaaaaggaat
      481 atagcaaagt gctacactcc tctattataa cttatttaac tgttaacata tactaattat
      541 acagaattcc tactagcaaa taatatcttt taattttaaa attaaactta caagttcttc
      601 ataggtatgt acatacattt cttttgtacc gccgtatgga tctataactt ctcctgcttc
      661 ttttacatat tcatgcaatg tgaaaacatg attttgcaaa ccaaagtgtg cctctattaa
      721 ttctttgtgc gaatacgaca tagtcaaaat aatatctgct ttcaaatctg cttcagtaaa
      781 ttgttgcgat aaggtcgttt cagctaaatg atgttcttca actaagtctt caacataatt
      841 cgaaacacct tgattgttca cagcgaatat acctcttgat tcaaattgat gatttggcat
      901 aacctctttt gcaatacttt ccgctaatgg gctacgacat gtgttacctg tacaaacgaa
      961 taaaatcttc atagttcaca tcctttaata atgtgattac ctgcagcttt taacatgcga
     1021 ttcataattg cttctgtatt atcattcagc tcaaagccgt atatatacgc cgctgaaata
     1081 ttttcatttt catcaagtga atgtaacaca tcataaagat tatgacttgc ttgtttaaca
     1141 tcattgtcat cctgacataa ttgaatgaat tgcgcttcac ttggtataaa cgccacctta
     1201 ttactcggca caataaaagc tatagaagac caatctttac cgtcatttcc aattttgctc
     1261 tcgatatctg taataattgt aagtggtgta ttgggtgagt aatgcttata cttcatacct
     1321 ggtgcaattg gctgttcagt atcattataa tcagcatggg cgatactatt cggaagtatt
     1381 tctgttatca ttgctgctgt tatagaacca ggtcttgcaa ttttataagg aaaagatgtg
     1441 caatctaaaa ccgtactttc taatccttct tcactttgtt cagcttgaac aataccatcg
     1501 atacgtccat tcaaatcttg atatacatga ttgaaagttg ttggtgaagg tctaccactt
     1561 aaattagcac ttggagcagc tagaggttca tttatgattt gtaataattg tctacctaca
     1621 gaatggcttg gcattctaac agcaactgat gataaacctc cagaaacttt tcgacataga
     1681 tagcctggct ttaacggcaa tataaacgaa atagggcccg gccagaatgc ctgcattaac
     1741 ttttctacgc gtggatccaa agtatatgta aaatctttta attgaccttt actgtgtata
     1801 tgaacaataa gcggattgtc agatggacgg cctttagctt catatatttt agctacagct
     1861 tcttcatctg tcgcatttgc tgcaagtcca taaactgttt cagttggtaa acctattaaa
     1921 ccaccgttta aaacaatgtc ttttatttca ttaattttag gatattgctg taaatcttca
     1981 ttatattctc taacatccca aattttagta tccaacttaa tcacgccttt cttatttatc
     2041 ataatacaaa gcaaaaagct atgcacttaa ctaatcatag caaaggcata acttttaatt
     2101 accatttaaa tgagacgatt cgatcgtggc catttatatc tttaataatg tcgatttttt
     2161 tgtcaggaaa tttatttaaa attattgatt taagtgcctc accttgattg taaccaattt
     2221 caaaaacaac tgggctgcct ttttccataa cgtgaggtaa atcttcaatg attgattcat
     2281 aaatagcata tccatggtta tctgcaaaca atgcctgatg tggttcgaat ctcgtaaccg
     2341 ttggagacat cgtaaccata tctttttcat ctatataagg tggattagat atcaagccgt
     2401 tcaacttgat gccttcttta attaagggct ttaatgcatc ccctgttaaa aactgtattt
     2461 gtgattgatg cttctcagca ttattacgag ccatattcat tgcttcaagt gaaatatcag
     2521 tagcaataac atttaaatcc ggcttttcac atttcaaagt aattgcaagt acaccactac
     2581 ccgttccgat atctacgatt gttgcatcat cttctaactg ttgtaagaaa tgcaacatta
     2641 cttcttcagt ttcaggtctt ggtatcaaac aatttgagtt tacatcaaac gttctaccat
     2701 aaaatgaggc aaagccaact atatactgta taggctctcc taataacata cgttgtaagg
     2761 ctaagtcgaa cttcataatc atcgctttcg gcatatcatc atgcatgtgg actacaaagt
     2821 ccgtacgcgt ccattgaaat acatctaaca ttaaccattc agctcgtgtt tgttcaaacc
     2881 ctttttgttg tgttaaatga attgcttcat ctaacttttc tttataattc accattatta
     2941 agttctttca atttatctgt ctgctctgat aaagtcagtg catctataat ttcttctaaa
     3001 tggccttcca taatttgccc taatttttga agcgttagac ctatacggtg gtctgttaca
     3061 cggctttgtg gataattata agttcgaata cgttctgaac gatcaccagt accgactgct
     3121 gatttacgtt gtgacgcata cttttgttgt tcttcttgaa ctttcatatc gtataaacgt
     3181 gcttttaaca ctttcattgc tttttcacgg ttttgaattt gagacttctc agaagatgtt
     3241 gcaatgacac cagttggtaa atgggtaata cgtactgcag agtcagttgt gtttacgtgc
     3301 tgaccacctg caccacttga acgatacgta tcgattttta aatcttcatt tctaatttca
     3361 atttctacat cttcaacttc tggtaaaact gccactgtag ctgttgaagt atgaatacgt
     3421 ccacctgatt ctgtttcagg cacacgttga acgcggtgcg caccattttc aaatttcaat
     3481 ttactatacg cgccattacc agaaactgag aaactaattt ctttgtaacc accatggtca
     3541 ctttcagacg cttctactat ttcagttttg aatccttgtg attctgcata ctttgaatac
     3601 atgcgcatta aatcaccagc aaaaatcgca gcctcatcac caccagctgc tgctcttatt
     3661 tctacaataa cgtctttgtc atcattagga tctttaggaa tcaataatat tttaagctct
     3721 tcttcaagat ttggaagttc agctttaata ccactacttt cctcttttaa catttctact
     3781 tcttctttat catcagtttc acttaacatt tcttcaatat cagctaattc ttctttttta
     3841 gctttatagt tacgataaac atctacagtt ttttgtaaat cagcttgctc tttagaatat
     3901 ttacgtaatt tatctgaatc atttacaaca tctgggtcac ttaacagttc atttaactgt
     3961 tcgtatcttt cttctacaat atctaattga tcaaacactt ataattcctc cttattatta
     4021 tcactaggtg ctacgatatg gtgcgcgcga caacgtggct cataactttc attggcacct
     4081 actaagataa tcggatcatc gattttagct ggtttaccat ttattaatcg ttgcgttcta
     4141 ctagatgaag aaccacaaac agcacaaact gcttgaagtt tcgttacttg ttcactgaca
     4201 gccatcaatt taggcattgg ttcgaacggt tcgcccctaa aatccatatc taatccagca
     4261 acaataacac ggtgtccatc tgctgatagt ttttctacta tacttacaat ttcatcgtca
     4321 aaaaattgta cttcgtctat tcctataaca tcaacattag ttaagtcgtg cgtcataatt
     4381 tcacttgctt tagaaatatt aatcgcttca atggcattac cattatgaga gaccactttt
     4441 tctttatgat atcgatcatc aatcgccggt ttaaatacaa cgactttttg tttagcgtat
     4501 atgccccttc ttaaacgtct tataagttct tcggatttac cgctaaacat actacctgta
     4561 atacattcta tccaaccgga atggtaagtt tcatacattg agagttccac ctttttcaaa
     4621 acataatcgc tttattatat catatttcaa atattcataa atgtcttttt cataattata
     4681 tcgatattgt acatgaacaa ttattttata aatttgtctt gcatatttat atacagccat
     4741 tctattttgt tgcttcttat tcgtgcgatg tttctactca atacaaactt taataaaggg
     4801 aaaaatttat cattatgtat taacaacatg ttattgagtc acttctttga acacagtcgt
     4861 aagtaaatac ccttactaca cttataaata aaaaagctgt ctacaattcc attataggaa
     4921 gcagacagct taacgattac tatattagtt gtttgattta agaccaaact ttttgttgaa
     4981 acgttccaca cgtccatccg cagcagcgaa tttttgacgt ccagtgtaga atggatgtga
     5041 atcagatgaa atatctaaac gaataactgg gtattctttt ccatcttccc actccatcat
     5101 ttctgaagat gtttttgtag aaccgcttaa gaatttgaag tttgtagtag tatctaaaaa
     5161 gattacttgg tgatactcag gatgaattcc ttgtttcatt attttcagct cctttgccct
     5221 gaaccatctg gaacagagtt atttgtgagt ttttacccaa tactgtgtaa ttataaaggt
     5281 aattaattca aaacgcaagc ccctatataa tgtttattaa attataggtc gacccgtttt
     5341 agtactttct tctgcagact tttgtagctg cttgaagaaa tcttcattat tcttagacct
     5401 tttaagtttg cgaataaatc tttcagtaaa gtctgttgag tcagtgaata gatttcttaa
     5461 ttgccataat gtgtctaatt cagatttact tatcaacaat tcttctttac gcgttgaact
     5521 tctgccaata tcaattgcag ggaagatacg acgttcagac aatttacgat ctaaatgtaa
     5581 ctccatgtta cctgttcctt taaattcttc gtaaatcata tcgtccatac gtgaacccgt
     5641 atcaactaat gcagttgcaa gtattgttaa acttccaccc gcttcaatat ttctcgctgc
     5701 accgaagaat gcttttggtt tgtgtaaaga tgcaggatct aaaccacctg ataatgtacg
     5761 accacttggt ggaataacta agttataagc gcgtgctaat ctcgttatag aatccattaa
     5821 aataatgaca tcttccccaa tttctactaa acgctttgca cgctcaagta ataattcagc
     5881 tactttaaca tggtgttctg gtggttcgtc aaacgttgaa tgaacgactt cagcagcttc
     5941 tactgagcgt tctaaatccg ttacctcttc aggacgctcg ccaactaaca aaataaatag
     6001 ctttgcatct ggtttgttcg tactgattgc attcgctatt tcttttaata acgatgtttt
     6061 acctgcttta ggtggcgcca ctattaaacc acgttgacct aaaccaatcg gtgttactaa
     6121 atccatgatg cgcgttgaat aattttgtgt ttctgtctct aatttaatac gctcatctgg
     6181 ataaagtggt gtcaaagctt ggaaatgcgg tcgtttcttc acttcttctg cgttatggtc
     6241 attgacaaag tcaacttgta ataagccata atatttttcg ttatctttag gttttctaac
     6301 tttcccagtt actttatccc cacgtttaat ttcaaaacga cgaatttggc tagcagatat
     6361 ataaatatct ttttcccctt tagaatagtt cactgttctt aaaaaaccat aaccatccgg
     6421 ttgtatatca tctaagatac cttccatata atagttacca tctttttcca tttgtgcttc
     6481 cataatagct agaacaagtt cttttttatt taatttacta tagttcgtca acttaagagt
     6541 tttagctttt tgagtgagtt ccttggtagt atagttcttg tacaattcgt ggaatgattc
     6601 atactgagga gatgtacgtt ctctttcagg catatagttt acacccattt cattattttt
     6661 tagttagtaa gtaatagata ggcatgacaa tgcataaata actgtgctag cacaatccca
     6721 ttatgctaat ccccaacctt ttaaatacat gaataactat agcaaatttt tattttaatt
     6781 acaaaatcta tttataaaaa ttgcaaaatt aaaacagtac acaaaactta attagtcatg
     6841 tgcactgttt tattgcgatt tttaattcaa aaatcaaatt tatttaaaat agccagctat
     6901 agatttcact tctaagaact cttcaatacc ataatcgccc cattcacgac ctaaaccaga
     6961 ttgtttatag ccaccaaatg gtaagtctgg ctttctacct gcttcgttta tttctactgt
     7021 acctgcttca atagaacgag ctactttatg caatgtttct ttgtccttac caataacata
     7081 tcctgctaaa ccatattttg tatcatttgc aatttgaatc gcttcatcta aatcgttata
     7141 agtgataact gacattactg gcccaaaaat ttcttcttgt gctatcgtca tttgattatc
     7201 tacattaata aaaattgtcg gacgtgcaaa gtatcctttt tcaagtcctt ctggtttacc
     7261 aggaccacca taaaataatt cagcaccttc ttcaatacct ttattaatat aattttgtac
     7321 ttgatcaaat tgttttttac taatgatagg gcctacttgt gtaccatctt ctcttggatt
     7381 accgacacgc acttggctaa attgttcttt taattcagct aagaatgcat ctttaatttt
     7441 gttaggcact aaaacacgtg taccagctgt acatacttga ccagtattat taacaacttt
     7501 gcctgttgtt gctttagccg cttctttaat atctacgtca tctaggacga tgtatggtga
     7561 tttgccacca agctctaatg ataccttttt aaaatcttta gcagcttttt ccataatttt
     7621 agaaccagta gggcctgatc ctgtaaatga catcatgcgt actttaggat gttcagataa
     7681 aggattccca acgcctgcac catcaccatt aacaaggtta aatacacctt taggaacacc
     7741 gactttatca aaaatctcag ctaaaataac agctgcaaat ggtgtttctt cagatggttt
     7801 aagtacaact ggactaccag ccgcaaatgc tgctgctaat tttaatgatg tttggtttgt
     7861 agggaagttc cacggtgtaa ttaagccaga tacaccgatt gcttctttaa caactaaatc
     7921 atctccgcgg cgttcttcaa attcgtaatt atctaatgcg tctctcgctg caacaaaatg
     7981 gtttagtccc atttgataat ggacacgctc agataatgat aaaggagcac ctaactcatc
     8041 cgtaatagct tgtacaatat cgtcttttct gttttcatat tcttttacaa ttttatctaa
     8101 taacgcttgt ctttctttca cagatgtatg acggaactct aaataaacat tgtctgccgc
     8161 ctcgacggct ttatcaacat cagctttatt acctttagca actttcccga ttgcttcttc
     8221 ggttgctgga tttataactt ctatcgtttc attactatta ctttctaccc attcgccatt
     8281 aatgtattgc tttgtgtagt ctctcattat tctttcactc ctcaagaatg atttatatat
     8341 tatttatccg taatgcatac gatataaaca ttacattcca ttatgatacg ctccctttta
     8401 atatgttcaa aataaaatgc tttaaaattt tgctgcatct aattttttat atttttaaat
     8461 aaaaataact attcccacat catcgcaaat aatgcgaaga cttagaaata gttataatca
     8521 atgctatgtt taaacgtcat gtaatcattt agcagtacgt tgatctgtta aatcgacata
     8581 tgattgcgcc catgcttcaa ttggatgtaa agcctctgct aacgctttac ctttttcagt
     8641 tagcacataa ataatttgta ctggactcgt agaaatgatt tgcttctcaa ctaactccca
     8701 ttgtgcaagc tctgacaact taagacttaa agcacgtggt gttattgttt tcaaatctct
     8761 tttcatatcg gaaaagtgtg ctgaacagtc attacatctt gagagataat taatgattaa
     8821 tccattccaa cttctaccaa gtattttaaa agtttcttcg agatacggac atacttccat
     8881 catcttcacc tctcatcaat aaatttagaa tgtaaacgaa cacccttttt taatcagtat
     8941 aattattata ccacaaaaca agataaatct atacagtttc cgtccaaata tctgcaccta
     9001 aagcttttaa atgttctaca atatctgtat aacctctata aatatgttta acattgtaaa
     9061 ttgtagttac accctcagca attaaaccag caataattaa acaagctcct gctcttaaat
     9121 cgctagcata aacttcagca ccatgtaatg ttgatggttt tatcgttgct gtgccttcgt
     9181 caacttcaat atttgcaccc atgcgcttta attcttcaac atgtttaaaa cgctccggat
     9241 aaatcgtatc agttacaaat gaaggaccat ttgccataaa taataatggt gtaataggct
     9301 gttgcaaatc agtagcaaaa cctggatata ctagtgtttt aatatcaaca aatcgatatg
     9361 gcgcattatt attgatgcga attctttcgt ctcttacatc aacattcaca cctaattcac
     9421 taaatttagc agttaatgtt tctacatgtt tcggaacaat attatttaat ataacatttt
     9481 ctccacatgc tgcagcgata cacatatatg tgcctgcttc aattctatca ggtataactt
     9541 gatactcaga accatgtaat tctttgacac cattgatttt aattgttgat gtacccgctc
     9601 ccttaatatt agctcccata cttgttaaga agttagcaac atcaactact tccggttctt
     9661 tagcagcatt ttcaattaca gtttgtcctg ttgcataaac tgcagctagc ataatgttaa
     9721 ttgttgcacc tacgctaacc atatctagaa aaatatgtgc accttttaac tctttagctt
     9781 cgattttcat tgatgtcgta cttgattcat caatttcagc acctaaagct ttaaatcctt
     9841 taatatgttg atcaattgga cgtggcccaa gtggacatcc tcccggtaaa ccaatcacac
     9901 attttttaaa tctacctaac acggcaccca tcatatagta tgaagcacgt aatgattcaa
     9961 ctttattatt tggtaatgca gcattttgta tttcagttgt atcaacttct aattccgtac
    10021 catttagtga tgccttaata tttaaatctt ctaataaact cactaaagtt ttaacatcag
    10081 agatttgcgg taacccttct aatttcacat gtccttgagc taataatgtt gcaggaatga
    10141 tagctacagc actgtttttc gcgccactga tattaacttc cccatttagt gtgcgtccac
    10201 ctcttatttt tattacctct tgagccatat catttatctc ctttgtccat tactttttaa
    10261 ttttttgcat tattagcatt ataaattaat tttactttat ttgtaaaata gtttatttta
    10321 atcgttaaaa tcttgtcttt ctattttcga ttgattaata acaaatcatt cacctttact
    10381 aatatatgct gttcttcccc acttgaccat ggtctaaagt ctgattattc aactaaatat
    10441 tgacgttgtt ttgacaatta tttagtttta attctacggt tttcacgtaa acttttttca
    10501 taaattgctc attttcctta taattatatc acaatcaatt ttgatatatt ttaccatttt
    10561 tacgctttca ttttaaaatc aaactgtttt atttattact tatacaacat aaaaaagaag
    10621 caaacaccat tttgtctgct tcttttaaaa ttaatatccc tacgttatta ataacttaaa
    10681 gactaaatat taattattta gcgcggtttg aagtaccgaa ctctttaatt ttacctttaa
    10741 ctgtttcttt gatagcttca cgtgcaggtc ctaagtattt acgaggatcg taaacttctt
    10801 tgtcgttatt taaaacgtca cgaactgctt ttgctgaagc gatttggctt tcagtgttta
    10861 cgttaatttt agctgtacca aatggaattg ctttttggat atctttagtc gggataccag
    10921 taccaccgtg taatactaat ggtaaacctg tagataaacc gatttcttcc atttctttaa
    10981 atcctaattt tggttcacct ttgtatggac catgaactga acctaatgct ggcgctaatg
    11041 catcaatacc agttttttca actagttctt gacattcttt aggatcagca taaatgatgc
    11101 cgtctgctac aacatcatct tcttgtccac caacagtacc taattcagct tctacagaaa
    11161 cacctttttc atgagcgtat tcaacaactt ttttagttgt tgctacgttt tcttcgaatg
    11221 ggctgtgtga agcatcgatc attactgatg tgaaaccagc atcgatagct tctttacatt
    11281 tttcaaagct tgaaccatgg tctaaatgga ttgctacagg aatagtgatg tttaagtcat
    11341 gcattaaccc ttcaaccatt ttaacaattg tgtagaaacc gctcatgtaa cgagcagcac
    11401 cttcagaaac acctaaaatt acaggtgcat tttcttcttg tgacgcttct aaaattgctt
    11461 gagtgaattc taggttatta atattgtatt gacctaccgc ataaccattt tcttttgcat
    11521 caattaacat ttctttcatt gaaactaaag gcatgaaagt tcctccttga gtgcttacgc
    11581 acgtattttt caaagtaatt atatcaaaaa aatagcgtca atgcattgaa atactgcaga
    11641 ttaaatgtgt attttttgaa aaatgcgata gttatactaa atgcaaacgt atacattttt
    11701 atacaattta agccgttgca taatatgtag tcattaatta caatatttta taagtagatt
    11761 ggaggataaa gatgtcaaaa atcttaaaca cacaactaat aggtatcttt aataggctag
    11821 aaaaacaatc tttagaaatt caaatggccg cccaatgttt aattcaagca attggtggcg
    11881 aaggttatgt ttatgtaaaa ggttatgatg atttacagtt tttcgaaagt ttcattctac
    11941 atagtgatga gcgtttaaaa tctagccgaa aacttgacgc tattaaagac tttaaagaaa
    12001 ttgactcaac tgatcgtgta ttattattcg caccatttta taatgatcaa gttgcattag
    12061 atattcaaaa acttatcgcc ttagatatcg atgtggtctt aataagcaac aaacctaaaa
    12121 cagatgattt ccctgatcat ctcgtacatt ttatcgattt atctacacca agacctatag
    12181 tctatactga agattacgat aagattgtcc agccacatgc gatggcattg aattatgtat
    12241 actatgacat ttatacacaa atgattgaaa tgacaagaga cttagaatta taaaagtcac
    12301 tattgttatt taataccttg tcactaacat aatattaaaa ttataagaaa aaccggtact
    12361 ttcaatgttt aacaatgaaa gtaccggttt tattagcaaa tttatttatt ttgttgatat
    12421 tttaatgaag cttcaatgaa tgatttaaaa atcgggtgcg gacgatttgg tctagataag
    12481 aattctgggt ggaattgaca agcaataaag aaatcatttg tcggaatctc taccatttct
    12541 actaaacgtc catctggact tgcaccagaa attaccatac catttgcttc taattgttct
    12601 ctatagtcat tattaaattc ataacgatga cgatgtcttt cttcaatttc cgctttaccg
    12661 taaacatctt gtgccaatgt gccttcttta attgaacatg gatataagcc taagcgtaat
    12721 gtaccaccta aatcttcgat atctttttgt tctggtaata aatctataat tgggtatggt
    12781 gttgctgggt ctaattcagc tgaatgtgcg ccttcaaggc ctaatacgtt tcttgaaaat
    12841 tcaactgttg caagttgcat tcctaaacaa ataccaaaga atggtacatt gttttctcta
    12901 gcatacttaa ttgcactaat tttaccttca cttgcacgga aaccaaatcc acctggtact
    12961 aaaataccgt cgacatctgc aaggtattcg gccgcatttt catctgtaac ttcacttgaa
    13021 tcaatccatc taatatcaat atctttagcg aaaggatatc cggcatgttt taatgattca
    13081 actacagata aatatgcatc ttgtaagcta acatacttac caactaaacc aattgtaatt
    13141 ttaccatcca aattattaac gatatctaac aactgtttcc attcatcaag ctgtgtttca
    13201 tatttcgcgt ttaattgtaa acgtttaata acgatatcgt ccatattttg ttggcttaat
    13261 tgtaacggaa tttcgtataa agaatctgca tcacgacatt caataacact ttctttatta
    13321 atgtcacaga ataatgcaat tttatctttt aaatcttgtg tcatttcata ttcagttctt
    13381 acaacgatta agtctggttg aatacctaag cctcgtaatt ctttaacgct atgttgtgtt
    13441 ggcttcgttt tcatttctcc agcagcttta atataaggca gtaatgtaca gtgaacatac
    13501 ataacatttt ctctacctaa atcgctacga atttgacgaa tcgcttcaat aaacggtaat
    13561 gactcaatat cacctgttgt accgccaatt tcagtgataa caacgtctgc attcgtactt
    13621 tcccctgcaa gtaataaacg ttctttaatt tcatttgtga tatgcggaat aacttgaact
    13681 gttccgccta agtaatcacc acgacgttct tttttcaata cgtgtgaata cacttttccg
    13741 gctgttacat ttgaaaactt gtttaaatta atatcaataa atctttcgta atgtcctaag
    13801 tctaggtcag tttctgcacc atcatccgtt acgaatactt caccatgttg ataaggactc
    13861 attgtacctg ggtcaacatt taagtatgga tcgaattttt gaattgttac atttagacct
    13921 ctatctttta ataatctacc tagagaagat gctgtaatac ccttccctaa tgatgaaact
    13981 acgccacctg ttacaaaaat aaattttgtc atgtcttgac ctcctaatta ttaaagggaa
    14041 tgcttcctca tcaattgtaa aaattacgta aaacataaca tttgagtaag ctcattcaag
    14101 ttgttcattt gtcgaattcg acaaaattaa attctgttta cttaccgttt tcatactgct
    14161 atgtcactat gtatatcatt tacaactttg attattgcgt tatcatttct tgatttactc
    14221 aattaaataa aaaaacgctc cctctcacaa attttgtaag gggagcgtat aaattttata
    14281 cacgtgtcct atttaaagga gcccgaataa aattttatca tctttcaact aaaagtcaaa
    14341 caaaaaatta atcgttgaag tcttcttcgt cttcaaacac ttcttcatct tcgtctagtt
    14401 catcttcatc ttcttcaatg actatatccg aatgattgat ttcttcttca acctgctcat
    14461 cttctggatc atttagttct tcttgatcat ctgtttgagc tggaatatca tcgtcgtcat
    14521 ccatttcatc ttcgcccaat aattttaagt tttgatcttc ttcatcatct gcatccagaa
    14581 tatcgaattt ttgaatagtt ggtgcgattt tctcttcaat atcatctacc gaataccaat
    14641 cacgtaatcc ccataaattt tctccaacat ttaaaaaacg accatctgtg tttaaatctg
    14701 tgtaaaattg tacaacgcga ttttcaattt cttcgtactc ataatcacct aacgctctaa
    14761 actcatcgat gatatcatat aagttcatag tttcgccttt atcattcaat aatgtataag
    14821 ccatatcaat aaatgatttt tcatcaacca tttgttttgt ataatcttga attttcattg
    14881 cccaatactt cctttcgaag gatataatct aaatttatac gtctgaattc atttatatat
    14941 agtaacaaaa ctaaattata aatgacaata tcatatttcg caatcactgt tagtaattta
    15001 aatatttttc aaaaacgaca taatcatcag tttgttcgat ttcaacgaaa ttactattta
    15061 aacataatgg tttaaaatca ttattctctc caaacagaat catcgtaatc acattaaggt
    15121 tgtcatagtt ttttcttaag taagtaggca attgtcgaag cgcacttgaa ccaataccat
    15181 taaagcgata tgatttatcc acttcaatag cagcaatgtt catgcgttca ccaatctgct
    15241 ccaatgcgat aaagccaacc ttttggccat caatcattaa attaatgata tgtacattgg
    15301 tcacttctga acgactttct tcttcaagct ttaatgacga aacatgtttt gaatctaagt
    15361 ccaagtaata tagtcgttct ttgccaattg gtccttcttc tttagtcgct gtatgcatga
    15421 atccagcacg ttcgtatagg ttccaagcat tgtcattttc cgcatctact actagatata
    15481 gatgattaaa atcaggaaat acaccttgaa catattgcgg caatgacatc attattttcg
    15541 tgccatatcc aaaaccttga tatttttcat taatcgataa tgaacgaata taaacgacat
    15601 tttcaggtgt atcataacct tcatgctgat agtaacgatg taatacaaaa aagcccacca
    15661 cttcattttt atcgtttatt gctacgttag caatcctatc agcatcatta attgcatcat
    15721 caataacttc tttaggtaaa gatgaatata tttgttgtct ttcattcaag tcaaaatcat
    15781 taattgctga acgatattgt tcatcaaatg cttttaaagt gataccttca aaacaaagtt
    15841 ctttagccat atatttaacc cctatctttt tatatatgaa gcatgaatcc ccatattatt
    15901 ttacctatta tttcactacg ataccattat ttgctctaag ttaaataaat tacttattta
    15961 tattttttaa atttcaaaat taatgtgcta ttcctcatta cttatcattt taatcagaga
    16021 cttacaaata cactgcgtac gaactacttt taatattgcg tcactaacta acaatcctat
    16081 acaacttcaa aattcctaga aaataatata tataacttaa cgtctacgtt tgtaatacat
    16141 taattgcgat acgcttacaa tgttgaaata caatggcatc aaatttgtta ataattacta
    16201 ttattacttg ttttttagtc attttactat actattattt aaggatagac attaataatg
    16261 aggtgggaat cttatgaaag ttggcattga cgctggcggt acactaatta aaatcgttca
    16321 agagcaagat aaccaacgta cttttaaaac tgaattaact aaaaatattg atcaagttgt
    16381 ggaatggtta aaccaacagc aaattgaaaa attatgctta actggaggta atgcaggtgt
    16441 tattgctgaa aacatcaaca ttcctgcaca aatttttgta gagtttgatg ctgcatctca
    16501 aggactaggt attttgttaa aagagcaagg ccatgactta gcagattata tttttgcaaa
    16561 tgtcggtacc ggtacgtcac tacattattt cgacggccaa tcacaacgtc gtgtaggcgg
    16621 tatcggtaca ggtggtggca tgattcaagg actcggttat ttactaagtc aaatcactga
    16681 ttataaacag ttaactgaca tggcacaaca cggtgatcgt aacactatcg atttaaaggt
    16741 tagacatatt tataaagata ccgaaccgcc cattcccggt gatttaacag cagctaattt
    16801 tggtcatgta ctacatcatt tagatgctga ttttacacct agcaataaat tagcagccgt
    16861 tattggtgtc gttggcgaag ttgttacgac aatggccatt actgttgcac gtgaatttaa
    16921 aactgaaaat atcgtttata tcggttcttc atttcataat aacgctttat tacgcaaagt
    16981 tgtggaagat tacactgtgt tacgcggttg caaaccgtac tatgttgaaa acggcgcatt
    17041 ttcaggtgct attggtgcat tatatttagg aaaatagaaa aagaccattt atatgcgcgt
    17101 tgttttaaat agacgctata taaatggcct tttattttat ggtctaagtg ttttgaatac
    17161 gtattgttcc atttattatc acttaattac aactcaattc agttaaaatg taagttcatt
    17221 agacaatttt tgcgctgaaa caattgtacc aaccacgcca gtttttaaat ctatcgcaac
    17281 ggctttatga tcatctctaa gttcatctag ccattctccg aaaacagaac cttcaatcgt
    17341 aattaaatca cactcttcaa aatcttcatc tcgaactttt aacgctctgt ttttaatact
    17401 ccatacaggc ataatatgcg gctttttttc gtcgctatca ttttcactaa tgagaataaa
    17461 ctgcttacta ccttttttag ttaagccgta tacttcatca tatttcgcta catcttggac
    17521 aaattctttg attctaattt tatctaattc cgtcatcact tcttgtgtaa cagctacaac
    17581 gtttagtaga tgcccttctt cttccattgt ttgattcata ataatatggt cttgttcatc
    17641 aaacaattca tctaattcat aagtagcaaa acgatcaacg tccattttta caactttatc
    17701 aaatggaata tcacgacttg ttaaaaatga tacggccaca ctttcttctg tccaaacgcc
    17761 tacatacgct tcacttttat aatttaggcg tactagtttt ttatttttag aggcgaaata
    17821 aatacattca tttattaata tatctctaaa gaatggatat gctttataag acatgcttga
    17881 ccctcacttt ttaaaatatg tcatacataa aatagtaaat gactccaaca agttagtcaa
    17941 ttagacatat tatgaattca atttaatgtc atgtttttca ttttaaaaat tcaatagcat
    18001 taaattttac gtatacacaa attcggtcta taacacgaaa catatctagt tactaaaaat
    18061 ttcaaaacga aaacctttag tataccttta tcaacaaaat ggcaactctt ataacatagt
    18121 gaaataaaag tcgccattta atattagtat tacaaagtat tgccatatca taaatttaat
    18181 tactgttgac tagaatcgaa aaaagcttgt tacaaacgca tattcagtca actactggca
    18241 acacaacatt gtagcgcctt atgttccgaa ctctaatagt gttcaagttc agctaaaaat
    18301 gcgcgttcat tctccagtac tgtttctttt gaatcaccat gccaaataca gccaaacctt
    18361 gatttaactt tatcttcacc gtaccaatca ccatcataat aagataacgg gtataaacta
    18421 ccttctttga catatttcaa aatcgtattg aagaaatgtg tgttatcacc ttttgaatga
    18481 taactataaa acttttgata tcctgaattc aactcgttag caagtaataa catgctcgtt
    18541 gaaccatttt gtctaaagtt taaatcaatt gcataaacgt tatcatcctc atcgacgagt
    18601 aggtcaaaac cagctacacc aaagaatcct tgattcacac cgttttccat aatttgtcta
    18661 cctgcttcta taacgtgctc tggtacatta gtagtatttt catttccgtt ataaaatccg
    18721 tacttatccg ttaattgcgt tgccgcacca agatattgaa tacctagcga ttccgagtat
    18781 gcaaactgaa cgcaataatt cgctttttct tcaatcttct gttcgataat aagcgaattc
    18841 gtttcagcag ttgcctgttt aatacgcgtt atcgcttttt gtaaatctgc atcatgataa
    18901 caaatcataa cgccatagcc acctgctgtc ggaagatcat ctcccggctt aattacaaac
    18961 gggaattccc aattcttaat ctcgttttcg aattgctcaa tttttacaac ttttcttttt
    19021 ggtaaaaact tcccatttgt ccattcaggt attcttgctt tattatttaa agcaacaaat
    19081 aacgttttat ctaatgcata atattgctga ttcaagattg tttcatcatg aatatattga
    19141 aaataaatct ttttattttc cttatgtgcc aattgtttaa tcaagttttc gtaagattgc
    19201 tgattgttaa atgtataaat tgagttcggt acttccttac caataacttg aaatagctga
    19261 tgcaatttgt ctgtcgcact agcttcgtga acaataacag gtaattgatt tgctattaat
    19321 aactccctac cagttaaaaa attagattga tgttcgtccg gtttcaacca tggattcgat
    19381 atatacgaag gtcttgacgt atagacaaca tctttgtcat ataaatcact taacgttaag
    19441 ttcggctcat taccattatt tgtcattact tcccattccc tttcaaatgc gcatgctctt
    19501 caataatgtc ttgataaacg tcttgatttg taattaactc taaccccatc aacgccatta
    19561 ttttagcacc tttaattaat gcttcatcac catgtacact cgcagctgct tctctaaatc
    19621 tatgcgtatg tcctactaaa ttacgtgatc ctattttaat atgaggatgt attgttggca
    19681 caacatgact tacgttccct gtatccgtag agccataacc aaaatcatca tcaataactg
    19741 cttcaccaac ttcttcagca tatttagcaa ataaatcatc taatttcggc gttttaatga
    19801 attcattcac accgttttga attggaccaa attcataatc acaaccagtc tttatcgcag
    19861 ctccacgtgc gatttgattt actttttctg ttaatatatc caattcttta cgcgtcattg
    19921 ctctagtata aaaacgagca tgtgtatagt ctggaataat attagctgct ttcccgccat
    19981 ctaaaatcac accatgcaca cgttgatctt ttttaatatg ttgtcgtagt tgtgctacac
    20041 cattaaaata actaatcata gcgtctaatg catttaacgc ttcatctgca ttttcagagg
    20101 catgagcact ttttccgtaa aatttaacat ctaaaacatc cactgccaaa gtatcaatcg
    20161 ttttataagt ttcatttccc ggatgaatca ttaaggcaat gtctatttga tcaatcacac
    20221 cagccttgac ataagaagct ttagcgctac cattttcccc accttcttca gctggacatc
    20281 cgagaacgac tactttacca ccaatttggt caatcacttg cttcaaacca attgcaccaa
    20341 gcacacttgc agttccaatg atattatgac cacaagcatg acccaatcct ggcaaagcat
    20401 cgtattctgc taaaaaacct atagctggcc cgtcaagatc cgaatcatat gtcgctataa
    20461 acccagttgc atgtccagca atctcggttt caatttcaaa atcatgttct ttcaaacgat
    20521 ctattaaagt tcgagacgca aatatttctt cattaccaag ttcaggacgt tcatgaattc
    20581 tatgactgat ttcgatataa ctatatttat ttgtttctat ataatcgaga atttgttgtt
    20641 tttcactcat tttttactat ctccctttta ccctacactc attttaatca tccacaatat
    20701 tttgttcttt caaaatgaat tattacttat tctatcggtt ttatctcttg atgtcatcta
    20761 gtttttcttt atttacaaaa ttttctaata actaaaagcc ttcataaatt tataaaacag
    20821 tttcaaattg taaaacataa gccctattgt tacaatttaa gtattgttag aaacgcaact
    20881 tgcaacaaat ttaagtttaa taatgtaacg aatcataagc gttttatagt tttgagtagt
    20941 taataggagg aaatttaaaa tgacaaaaat gaatgttgaa agttttaatt tagatcatac
    21001 taaagtggtt gccccattta ttagattagc gggaacgatg gaaggattaa acggagacgt
    21061 cattcacaaa tacgacattc gtttcaaaca accaaacaaa gaacatatgg atatgcctgg
    21121 attgcattca ttagaacatt taatggctga aaatattaga aatcatagtg acaaagtcgt
    21181 tgatttaagt cctatgggtt gccaaactgg tttctatgta tcatttatta atcatgataa
    21241 ttatgatgat gtattaaata ttgttgaagc aactttaaat gatgtgctaa atgctactga
    21301 ggtgcctgct tgtaatgaag tacaatgtgg ctgggcagca agtcattcat tagaaggtgc
    21361 taaaactatc gctcaagcat ttctagacaa acgaaacgaa tggcatgatg ttttcggtac
    21421 aggaaaataa atcttagtca atcaagttaa tcagaaaagc agtcgaacaa tgatgttaca
    21481 atcgccattg tccaactgct ttttattatg cttcaaagtc aaaaaatcga acaaatgaaa
    21541 aagtaaaatc tttaacattt gtccgattta tttgagaacc actataattt cttaattagt
    21601 cccattaaca cgaactgcat aggtaacctt aaatatagtt gccatgttgg caattgttta
    21661 tctcctaaag gtaacttttt aactgccata tagatattag ctgggaatac agctagcaag
    21721 aatagattga ttgtattttt caagcattga gatggtcttt taattaaaag tgcaagtcca
    21781 aataatatct caaagattcc tgtaacaaga accgctgttt ttcgaagcgg caaacatttc
    21841 ggtatgatat ttctaaattg tcgttctcgt gtaaaatgca atacacctat tatactaaaa
    21901 cctattccta ataaatatct tagtatgttc aatcagcttc aactcctact ctgtaattat
    21961 tttatgaatt aatgtaggcg atacaacata atcagcaatt gttatgcttg aatctaattt
    22021 tttaacaaca tcgtctacat cttggcgatt actgtgaatc gttaataatg attctccttc
    22081 ttctacttta tcaccaattt ttttatttaa aacaatacca accgctaaat caatatcatc
    22141 ctcttttgtt aaacgtcccg ctcctaacat catcgaagcg acacctatat cgttagagac
    22201 taactcagtc acataacctg attttttagc tttatattcg atttgatatt gagcttgtgg
    22261 caaacgctct ggatggtcaa taactgtttt gtcgccacct tggtttttaa taaatgtttt
    22321 gaatttttct aatgctgcac ctgaatcaat tgcctcaatt agcaacgctc tcgcttcttc
    22381 aagcgtttca gctttgtttg caagtacaac catttgagaa cctaatgtta atacaagttc
    22441 tgttaaatct ttcggacctt gtcctttcaa cgtatcaatt gcttcttgta actcaagcgc
    22501 attgccaatc gcacgtccaa gtggctgatt catatcagaa ataatcgcca tcgtattacg
    22561 tcccacatta ttaccaatac gtaccattgc gtgcgctaat gcttcagcat cttctaatgt
    22621 tttcataaat gcaccgctac cagtttttac atctaataca attgcatctg caccagcagc
    22681 aatcttttta ctcataattg aagaggcaat taatggtatt gaattgacag taccagtaac
    22741 atcccttaag gcatataatt ttttatctgc aggagttaaa tttcctgatt gtcctacaac
    22801 tgccacttta ttttcattaa ccaatttcac aaatgttgct tcatctattt caacatgaaa
    22861 accatcaatt gcttctaatt tatcaatcgt accacctgta tgacctaatc cacgcccact
    22921 catttttgca acaggaacat ctacagctgc tactaatggt gctaaaacca atgtagttgt
    22981 atctcctaca ccacctgttg agtgcttatc tactttgaca cctttaatat cactcaaatc
    23041 tatcatatca ccagaattaa ccatagccat cgttaatgct gcacgctcat catcgttcat
    23101 atcttggaaa taaatcgcca ttgctaaact tgacgcttga taatcaggaa tatccccttt
    23161 aacatagccg tcaataaaga aattaatttc ttccgttgtt agtgtatgac cgtcacgctt
    23221 tttctcaata atgtctatca ttctcatttt tatcatcctt ttcttaaaaa gcttaggaca
    23281 aagcatctgc gctttctcta gtccattttt aaaagcacaa gcgaaaatta ttatagcaag
    23341 ctatcgattt catttttaat atcacaattt aaatgcgatg ttattattct taaatagatt
    23401 ggttataacg tcaaagtccc tattaaatta tcttagaatc atcatggcat ttatgatgtc
    23461 ttaaaactga tatcgacata cttatatatg gttacgatgt cccatgctta catatttttt
    23521 ataaaattag taatctgaat ctgcttctaa accttgca
//
LOCUS       DAMZPP010000025        23426 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMZPP010000025 DAMZPP010000000
VERSION     DAMZPP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23426
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.025"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..680
                     /locus_tag="POH73_000770"
     CDS             <1..680
                     /locus_tag="POH73_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDI6886745.1"
                     /translation="ALMMTPHHEVKRIVVYLRGGKGQVGRVRAGRLMQFSDSQTLVIG
                     PYYRGNNGSEGKDEFYRGDLNDVTELLRLLHDKYPQAFIHMVGFSRGGLQGLLTFQDL
                     PVNSYIIWGGVSDIDLMYEERVDLRGMLRRMIGHPKKDRAAYEARQAIPNINENSPAI
                     LIVHGGKDQQVGIHHAYYLADQLELKGAMHETFYQMAEGHVPRPPAMVETLTYIKEFM
                     NQVELHR"
     gene            complement(973..2565)
                     /gene="pckA"
                     /locus_tag="POH73_000771"
     CDS             complement(973..2565)
                     /gene="pckA"
                     /locus_tag="POH73_000771"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDI6886746.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKTQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     regulatory      2720..2831
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2937..4130
                     /locus_tag="POH73_000772"
     CDS             2937..4130
                     /locus_tag="POH73_000772"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDI6886747.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHADDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     gene            4255..5163
                     /locus_tag="POH73_000773"
     CDS             4255..5163
                     /locus_tag="POH73_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HDI6886748.1"
                     /translation="MDLSSPVVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKHLGAKYTPTSILYFNPKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            5375..6208
                     /locus_tag="POH73_000774"
     CDS             5375..6208
                     /locus_tag="POH73_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6886749.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNKVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQHIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHGVVTIPKSVTPNRISENFRIFDFELSDEQMTRIDGLNQDKRIGPDPKTFE
                     G"
     gene            complement(6458..6811)
                     /locus_tag="POH73_000775"
     CDS             complement(6458..6811)
                     /locus_tag="POH73_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDI6886750.1"
                     /translation="MISIILVMIGGGLGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
     gene            complement(6808..7173)
                     /gene="crcB"
                     /locus_tag="POH73_000776"
     CDS             complement(6808..7173)
                     /gene="crcB"
                     /locus_tag="POH73_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDI6886751.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGGLS"
     gene            complement(7428..7730)
                     /locus_tag="POH73_000777"
     CDS             complement(7428..7730)
                     /locus_tag="POH73_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886752.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVHTFGFI"
     gene            7989..8702
                     /locus_tag="POH73_000778"
     CDS             7989..8702
                     /locus_tag="POH73_000778"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDI6886753.1"
                     /translation="MAKLNVEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
     gene            complement(9159..9779)
                     /locus_tag="POH73_000779"
     CDS             complement(9159..9779)
                     /locus_tag="POH73_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDI6886754.1"
                     /translation="MFSRNNNIFVDDLSVTKDNYLIAVLKMLIIFLVINYGTDISYDH
                     EYVGLFIILLGVVLLKVLNINLLSFKKLKFPHMLYIIFGFLLMYGLDYLYDTFAPPTL
                     NEILIDEESEDAPYHIALLSTAIIPPITEEIICRGLIIRILFRNHLFLGFIVSTVFFT
                     LIHESNTLIGYLPYFYSGLIFGYTYLKTKRLEVPILIHFINNLLAM"
     gene            complement(9946..10581)
                     /locus_tag="POH73_000780"
     CDS             complement(9946..10581)
                     /locus_tag="POH73_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDI6886755.1"
                     /translation="MQKFKDLFYDDLSVARGNYFLTLLSAFLITIILLIGFVVSDTHI
                     LYGVFIVLIGIVLLRLFKINLLSFKKLTMSQVIYIIGGALLIYGLDHLYLHFHDVPTN
                     EQELDRELQHMPLYMSIVTIAIIPAIVEEIVFRGMIIRVVFRKHLFIGLVVSSLVFAS
                     LHESDTWIGYLPYLYSGVIFGLIYLKTKRLEVVILIHFINNLSSLLILIWG"
     gene            10879..11322
                     /locus_tag="POH73_000781"
     CDS             10879..11322
                     /locus_tag="POH73_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886756.1"
                     /translation="MNKASFDKKVKKQLWFLNKNEKQALDQRLSSITDKDNVNFNKPI
                     TFANTYLRENVFRSKETKSYSIFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVNPK
                     VELSTTVVILTIIGAILLMLVSIYLIKITTSYFTKKLLEHKFNSH"
     gene            complement(11309..11752)
                     /locus_tag="POH73_000782"
     CDS             complement(11309..11752)
                     /locus_tag="POH73_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDI6886757.1"
                     /translation="MQDISTKHLLYIQTATSNHLETKCVFLYHEYNLEFPITKAIPYY
                     AKLHFTSASILIDAAKSILNINKLVPIYLNPKTILFPIKQKRAPIQTYINAHYIIGMT
                     EMGNSTMLHFKNGKTLEVDEPMSLISKKCYESLALKHFIENTNDY"
     gene            complement(11865..12335)
                     /locus_tag="POH73_000783"
     CDS             complement(11865..12335)
                     /locus_tag="POH73_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDI6886758.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSNQSLSSFLFMRLNFYLIDLFRQQKRLNDIILCENHSPILTEQPTYFNEYDLRL
                     QDVCALLNHRERQWLMLYLEGYKQYEIADIMSLSTSTIKLIKASVKRKCQRFFK"
     gene            12534..12758
                     /locus_tag="POH73_000784"
     CDS             12534..12758
                     /locus_tag="POH73_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886759.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            13034..13888
                     /locus_tag="POH73_000785"
     CDS             13034..13888
                     /locus_tag="POH73_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDI6886760.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(13975..15267)
                     /gene="arsB"
                     /locus_tag="POH73_000786"
     CDS             complement(13975..15267)
                     /gene="arsB"
                     /locus_tag="POH73_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HDI6886761.1"
                     /translation="MMTTLATFIFLVTLLFVLWQPKGLDIGFTALAGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGSGLKMFVYIIL
                     LGAVVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFNISFSQYLSRMIIPNLFSLLASLLVLWLYFRKAIPKSFDANHIKKPID
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLILARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFFTLLGLYLTLILF"
     gene            complement(15267..15581)
                     /locus_tag="POH73_000787"
     CDS             complement(15267..15581)
                     /locus_tag="POH73_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDI6886762.1"
                     /translation="MTYKELATYLKILSDSSRLEILDLLSCGELCACDLLEHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            16224..17726
                     /locus_tag="POH73_000788"
     CDS             16224..17726
                     /locus_tag="POH73_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDI6886763.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMYTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSEQSWQTYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSANLPSVRGTMLDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECAEYDI
                     DFEKLVHRRTGNHIADLKYDLARPTEMGIFQSIIEYLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YNVVINATGAKSHLNELDQDDQLIKNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQEQRSDNQ"
     regulatory      17945..18080
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            18207..19250
                     /gene="ribD"
                     /locus_tag="POH73_000789"
     CDS             18207..19250
                     /gene="ribD"
                     /locus_tag="POH73_000789"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDI6886764.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETIRDHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRHTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDKFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     gene            19257..19889
                     /gene="ribE"
                     /locus_tag="POH73_000790"
     CDS             19257..19889
                     /gene="ribE"
                     /locus_tag="POH73_000790"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDI6886765.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINKQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            19900..21081
                     /gene="ribB"
                     /locus_tag="POH73_000791"
     CDS             19900..21081
                     /gene="ribB"
                     /locus_tag="POH73_000791"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDI6886766.1"
                     /translation="MQFDNIDSALMALKNGETIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHKLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            21094..21558
                     /locus_tag="POH73_000792"
     CDS             21094..21558
                     /locus_tag="POH73_000792"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDI6886767.1"
                     /translation="MNFEGKLIGKELKVVIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHFDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(21680..22681)
                     /locus_tag="POH73_000793"
     CDS             complement(21680..22681)
                     /locus_tag="POH73_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HDI6886768.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAAKEFVKPAGLKRVGIIAALGATVMLCLSTIKKLCRK"
     gene            22793..22912
                     /locus_tag="POH73_000794"
     CDS             22793..22912
                     /locus_tag="POH73_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886769.1"
                     /translation="MMLISFVYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            22915..>23426
                     /locus_tag="POH73_000795"
     CDS             22915..>23426
                     /locus_tag="POH73_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6886770.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLK"
BASE COUNT         8444 a         3296 c         4171 g         7515 t
ORIGIN      
        1 aagctttaat gatgacacca catcatgaag taaaacgcat tgtagtatat ttgcgaggtg
       61 gcaaaggcca agtaggacgt gtgcgcgcag gtagattaat gcaattttca gatagtcaaa
      121 ctttagtgat tgggccatac taccgaggta acaatgggag tgaaggtaaa gatgaatttt
      181 atcgtggcga tttaaatgat gtgactgaat tattgaggtt gctacatgat aaatatccgc
      241 aagcttttat tcatatggtt ggtttttcaa gaggtggact acaagggttg ttgacgtttc
      301 aagacttacc tgtaaatagt tatatcatct ggggtggagt ctcagatatt gatttgatgt
      361 atgaagagcg cgttgattta agagggatgt tacgtagaat gattggtcat ccgaaaaaag
      421 atcgagcagc atatgaggca cgccaagcga ttccaaacat taatgaaaac agtccagcaa
      481 tattaattgt acatggaggg aaagaccagc aagttggtat tcatcatgcg tattatttag
      541 cggatcaact agagttaaaa ggtgccatgc atgaaacatt ttatcaaatg gcagaaggac
      601 atgtgccaag accaccagcg atggttgaaa cattgactta tattaaagaa tttatgaacc
      661 aagttgagtt gcataggtag tatgaaacgt ggtgtgcgca tgggaactat caatctttgg
      721 agatgtgtga tatacgttga gtgaggaatc ggtactttat gcaaagtggg gcaacatgca
      781 tagcgtaagg aatcggtact ttatgtgaag acgtgctgct tccattgaat gagtgatgtg
      841 agtttggcat gaaggtcacc ttaaatgtga ttgttgtaat aaaattgcct ataaattttt
      901 accatataaa ataagaggag ccaaccattg ttaaaattta acagcggttg gctctttaat
      961 tagtatttaa atttatttgt tgaagctacc tttttcggca atatgttcaa ctttttcacc
     1021 aaatttttcg aagttctttt caaaacgttg aattaaatct tctgcttgtg ttttatattt
     1081 ctctttgtcg ctccaagcat taattggatt taaaatagtt ttcggtacat cttcaatttc
     1141 tacaggaatg cttaaaccaa acgtactatc ctttgtatat tctgcatttt tcaatttgcc
     1201 agaaatcgct tggtttacca tttgacgtgt gtaatgtaag ctgattctac gtcctacacc
     1261 atattttccg ccagtccatc cagtattaac aagataaaca tcaacatcat gtaaatcgat
     1321 aagttcacct aataaatcag cgtaaacagt agggtgtaac gggaagaacg gtgcgccgaa
     1381 acatgttgag aatgatggtt caggttctgt cacaccacgc tctgtgccag ctaatttaga
     1441 agtgaaacca ctcaagaaat gatacattgc ttggtcttta tttaactttg aaatcggtgg
     1501 aataacacca aatgcatccg cagttaagaa aataattgta tttggatgtg ctgctttaga
     1561 tggtactaca atattgtcaa tgtgattaat tggataagcg gcacgcgtgt tttctgtata
     1621 acgattgtct tcaaagtcca ctgaaccatc ttctgcaact acagtgttct ctaaaattgc
     1681 accatatttg attgcgtcaa aaatctgtgg ttctttttct ttggaaagat taattgcttt
     1741 tgcatagcag ccaccttcga tattaaagac cccgttttta ttccagccgt gttcatcatc
     1801 accgattagt ttacggtgtg ggtcagccga taaggttgtt ttacctgtgc cagatagacc
     1861 aaagaataat gcaacatcgc ctttttcacc aacgtttgct gagcaatgca tgctcataat
     1921 atcttgcatc ggtaagagat aattcattac agagaagata ccttttttca tttcaccagc
     1981 gtattcagta ccgccgatta aaatgacttt gtgtttaaat gaaataatga caaaggtttc
     2041 agatttagta ccatcaactt ctggatctgc tttaaaatgt ggggcagaaa cgatagtgaa
     2101 gttaggttta atctttgtag cttcttcttt tgattcaggt ctaataaaca tatttttagc
     2161 aaataaatta tgccatgcta attcattgat gactgtaagt tttaacattg tatctttatc
     2221 gctaccagcg tagcctttaa atacgtataa ttcatctttt ttatctaaat agtctagtac
     2281 tttatggtat aacttcaaga aagtttcttc atcgataggt tgattaattt ctccccagtc
     2341 aatgttatct ctatatgaag gttctgagac aaaaaatttg tctttaggcg aacgaccagt
     2401 atattttcca gtacttgcat taacagcacc aagttctgtt aatacccctt cgttattgtc
     2461 taagatttta ttataaagtt gtgtcgtcga aagttgaaaa tgtgacgttg gttttttcag
     2521 taatttgtca attttagttg tttcagtgta tgtgtctact gacatcctaa atccctccaa
     2581 agcgataatt taaatgtaag cctttacatt taattagtat aacacattaa cataattaat
     2641 ctacaatatt ttactgtaaa tttatttgtg aaaaattgac attaattcat taattaggta
     2701 gtattatttt taacggattc tcttatcctg agtggtggag ggacatggac ccaatgaaac
     2761 ccagcaacct ctttttttat aaaagaaagg tgccaaaccg tttgcagaca aataggtctg
     2821 aacgataaga gcgaatggac gtattaggcc ttctctctat attaatagtt agaaggtctt
     2881 ttttatttag ctcacagaga gagaattttc gtaatataaa tttaaaggag caaactatgt
     2941 taaataacaa acgattattt acttcagagt ctgttacaga aggacaccca gataaaatcg
     3001 ctgaccaagt gtcagatgca atattagatg ctattttaaa agacgatcca aatgcacgtg
     3061 tagcttgtga aacaactgtt acaacaggta tggcattgat cgccggcgaa atttctacaa
     3121 caacatatgt tgatattccg aaagttgtta gagaaacaat caaagaaatt ggatacacaa
     3181 gagcaaaata tggttatgat tatgaaacaa tggcaatttt aactgcgatt gatgaacaat
     3241 cacctgacat tgcacaaggc gtggataaag cattagagta tcgtgataaa gatagcgaag
     3301 aagaaattga agcaacaggt gcaggtgacc aaggtttaat gtttggttac gcaacgaatg
     3361 aaacagagac gtatatgcct ctagcaattt atttatcaca tcaattggca aaacgtttat
     3421 cagatgtgcg taaagatggt acgttaaatt atttacgacc agacggtaaa gttcaggtca
     3481 ctgttgaata tgatgaaaat gataaccctg tacgtattga tacgattgtc gtatcaacac
     3541 aacatgctga tgatgtgaca cttgaacaaa ttcaagaaga catcaaagcg catgtcattt
     3601 atccaactgt tccagaaaac ttgattaatg aacaaactaa attctacatc aatcctacag
     3661 gacgctttgt aatcggtgga cctcaaggtg atgcaggatt aacaggacgt aaaatcattg
     3721 ttgatacgta cggtggctat gcacgtcatg gtggcggatg ctttagcggt aaagatccta
     3781 caaaagtaga ccgttcagct gcatacgctg cacgttatgt ggctaaaaat attgtagcag
     3841 caggtttagc agatcaatgt gaagtacaat tggcatatgc gattggtgtt gcagaaccag
     3901 tgtcaattgc aattgataca tttggaacag gtaaagtttc tgaagggcaa cttgtcgaag
     3961 cagttagaaa acactttgac ctaagacctg caggtattat taaaatgctt gatttgaaac
     4021 aaccgattta caaacaaact gctgcttatg gtcattttgg acgtacagat gtcttattcc
     4081 catgggaaaa attagacaaa gtggaagaat taaaagacgc agtaaaataa taagatgaat
     4141 tgaatatagc taaataaaag attttcggta tataaatgat aatatgtatc gaaaatcttt
     4201 agcttttcta ttataattaa agttattcat atattaaaaa ggagcgttgt tgtgatggat
     4261 ttatcttcac cggtagtcat tggactcatt attgcagtag tcattgcatt aatctttttt
     4321 gtccttttct tagttgcctt aggtagtaag aaaaaagtta agcgacaaac agaagaaaag
     4381 tatgaacaac aagaacaaaa cattaaaaag tctcatgaag aagctttaga gaaagagcgt
     4441 attcaaaata aaaagacaat tacaaaacaa caagaagatt ataatcacat ggttagtaca
     4501 aaagatcgtg agattgacgc attaaaattg ttctcgaaaa atcatagtga atatgtaacg
     4561 gacatgagat taattggtat tcgtgaaaga ttagttaaag aaaaacgaat tcgaccagaa
     4621 gatatgcaca ttatggcaaa tatcttttta ccaaaggatg gctttaataa tattgaacga
     4681 attagtcatt tagtattaac aagaactggt ctttatatta ttgattctca attgttaaaa
     4741 ggtcatgttt ataatggtat aagtggcggt caatttaaag atttaccacc tatggaacaa
     4801 gtatttgata cattggattt agataaatct agaccgcaaa ctattgttat ggatcaaaat
     4861 gatgacaaac gttcattatc atttgtaaat tattcagatc aaattgaggc aattaaacaa
     4921 ttggctgaag acttacaaaa acatttaggt gctaaatata caccgacttc aattttatac
     4981 tttaatccta aaaatgaagg cgatgtaaca atttcaaatt ataatcaaaa tagtgctgtc
     5041 aaagtgcttg tcggtgctga acaattagac gagttcttta acaaatttgt attccatggt
     5101 cgtattcaat acaacgtgga agacttacaa caaatgatgg ataaaattga atcattcaat
     5161 taaatgttag gctgaagtga tgactatatg ttgttacttc ggctttttta gtttgtcata
     5221 aattaaacag aattactttt acaatttgat taatatttga gagatattac tagcatttta
     5281 gtggttgatt gctctaagtg ttttttctca tgtattcaac tcagatttgt tatgataaca
     5341 gggtataata catcaaaaag gtgaggcgat atttatggag gttaaaacat tttataatgg
     5401 aaacacgatg ccacaaattg gtttagggac atttcgtgtg gaaaatgatg aaaattgcat
     5461 ggaaagtgtt aagtatgcca ttgaacaagg atatcgtagt attgataccg caaaagttta
     5521 tgggaatgaa gaacaagtag gtgctggcat tcgtgccgga ttggaatcaa ctggtattgc
     5581 tagagaagac ttatttatta cttcaaaatt atattttgag gattttggtc gtgaaaatgt
     5641 agcagctgcc tacgaagcta gtttatctag attaggttta aaatacttag atttgtatct
     5701 agtacattgg ccaggtacga acgaagccgt aatggttgat acatggaaag gtatggaaga
     5761 tttatataaa aataataaag ttaaaaatat aggtgtcagc aattttgaac ctgaacattt
     5821 ggaagctttg ttggcacaag tgtcaattaa acctgtaatt aatcaggttg aatatcatcc
     5881 atatttaacg caacataaat tgaaattata tttggcagca caacatatcg tgatggaatc
     5941 ttggtcacca ttgatgaatg cacaaatttt aaatgatgag acaattaaag acattgctca
     6001 agaattagga aagtcacctg cccaagttgt tttaagatgg aatgtgcagc atggagtggt
     6061 tacaatccct aaatcggtga caccaaacag aatctctgaa aacttccgaa tatttgattt
     6121 cgaattatca gatgaacaaa tgacgcgaat tgatggttta aatcaagata agagaattgg
     6181 acctgatcca aaaacttttg aaggatagat taaaatcgct caactgatga aaaggtcaga
     6241 taaattgtca acgttaggta atagacatat tgcttaacgt ttttattttc gactagtttg
     6301 gagaatttat tgacattcac attatttaaa cggcaacaaa gattgtttta ttttgatagg
     6361 cattatctgg tgctaaaaaa tttgcatgaa aattaaaaaa tgcttcgttc aggaaggtgt
     6421 cgtaatttac ctatttgctg aatgaagcat tttattttta aatatgatag ccaatataac
     6481 aagctataaa tccaatgatg aattgtaaaa gtgaataatt gagaaaaagg ttaatattaa
     6541 attttggcgt catcattagt gtaagttcct tggctaatgt tgagaaagtt gttaagccac
     6601 ctaaaaaacc ggtaacaaag aacgcaggga accatgagat tgaaattgat aagcctatag
     6661 ttaatccaat taaaaaacta ccaacaagat ttactatcaa tgttgcgata ggtaactttg
     6721 aagtgaattt atgattgaaa taatcagtaa tggcacttct agcaattgca cccaaaccgc
     6781 cgccaatcat gactaaaatg attgatatca tgataaacca ccacctagtt ttataccgac
     6841 gtaacataac aaaataccaa agacataact tgttacagca tatagtagta aagttataaa
     6901 ttgttgatga tcaaacatat gtattaattc taattgaaat gttgaaaaag tcgttaaagc
     6961 accaagaaaa ccagtcgtaa tagctttttt aagggtcgga tggtttgaaa aaaatgcaat
     7021 tgttaaggct gttagcaatc ccattacaaa agcaccagtc aaattggcta tcagtgttcc
     7081 gattggaaaa cctccgtcag tattcagaaa agaaatgagg taacgtaata aagcgcctaa
     7141 agcaccaccg ataaaaatat atacatattg catttggttc acctcgaaaa gaagtagttt
     7201 gaatttaaaa aagaggtttt ggcaacgcga cgacaaaaat tgtcgatgca ttatcaaacc
     7261 tcattatatg ttatatcttg ttgtataact atagcgatta gatgcatagt tatgatttcg
     7321 aaaatctaat atttttatac gcaacaacgt catcaaatgg atttactcat tttagcatga
     7381 tacattgtat tgttttgtat taaggctaca ttgacatttt atctttttta aataaaaccg
     7441 aatgtgtgaa caatcgaaaa gatatgtact aaaataacga ttagaataat ccaaggcaaa
     7501 cttttactcg caattctaat ccaatctgca tcaggcttta gtgatttaat tgaacgatct
     7561 gcaaaaatta tagacaatat tagtacaatt gagttaataa cactgcagaa aagtattaat
     7621 ttaataaaag aattaaaaag cccacttagg aaaacgttat ttgtattaaa gaaaaagctt
     7681 aaaataatca ttaagctaga taagtaaaag tatttttttg aacgagacat gctagacctc
     7741 ctggatggtt atcatttaca tatcgtatca taaacaatca taaaacaata gtttaattac
     7801 cttgttgaca caatgttgct aaagtcattt ttaagaaata taggcaaaaa attagctata
     7861 tattataaaa gcgtgatata aatgttttat ataacaaaga aataaaaatc attttttaca
     7921 aatggttgta agaaaaagac atgcagatgt tgttaaaatt ttaataagaa tcaaggaggc
     7981 tatattatat ggctaaacta aatgtagaag tatttgcgga tggtgcagat attgaagaaa
     8041 tgaaagcagc ttataaaaac aaacaagtgg atggctttac aacaaatcct agtttaatgg
     8101 cgaaagcggg cgtaacagat tacaaagctt ttgctgaaga agctgtgaaa gaaattccag
     8161 atgcttcaat ttcatttgaa gtatttgcag acgatttaga aactatggaa aaagaagcag
     8221 caattttaaa acaatatggc gaaaatgtat ttgttaaaat tcctattgta aatacaaaag
     8281 gtgaatcaac gattccttta attaaaaaac tttcagctga caatgtgaga ttaaacgtta
     8341 cggctgttta tacaattgaa caagttaaag aaataactga agcagtaact gaaggtgtgc
     8401 caacatatgt ttcagtattt gcaggacgta ttgcagatac aggcgtagat ccattaccat
     8461 taatgaaaga ggctgtaaaa gttacgcata gtaaagacgg cgttaaatta ttatgggcaa
     8521 gttgccgcga attattcaat gtgattcaag ctgatgaaat tggtgcagat attattacat
     8581 gccctgcaga tgttgtgaaa aaagtgaata caaacttagg tcgcgatatt aacgaattat
     8641 cagtagatac agttaaaggc tttgcgaaag atattcaatc ttcaggtctt tctattttat
     8701 aatatataaa tatatttaaa ccctcatagg cattgtgctt atgggggttt ttcatattgg
     8761 agtcaatttt aaattaatat gtatttcatt gcctgtagaa tttcttgtcg aaattctctg
     8821 tgttggggcc ccacacccca acgcgcattg cctgtagaat ttcttttttg aaattctcta
     8881 tgttggggcc tctataattg aaaagcttat tatcattcag tcaatgactg ttaatataac
     8941 agtatagaga gtctgggata ggctcgataa caataaaaaa tagctatgaa aaaaatctat
     9001 cgtcatagat tttttcatag ctaaacttaa tatgttttgt tgttttgata taattacaga
     9061 acgtaaatat gcaataacat ttggaaagtc acctattaat actttgtaaa agctttggtg
     9121 tcatcatgta aatatcaaaa taattaatga ttttattgtt acatggctaa gaggttattg
     9181 ataaaatgta ttaaaatcgg tacctctaat cgttttgttt ttaagtaagt atagccaaaa
     9241 attaaacctg aataaaaata cggaagataa ccaatcagcg tgtttgattc atgtattagc
     9301 gtgaagaata cagttgaaac tataaaacct aagaaaagat gatttctgaa taaaattcgt
     9361 ataatcaatc cacgacagat gatttcttct gtaattggtg gaatgatagc agtactaagc
     9421 agagctatgt ggtaaggtgc atcttcagac tcttcatcta tcaatatttc atttaatgta
     9481 ggcggtgcga acgtatcata taagtaatct aatccataca tcaacaaaaa tccaaatata
     9541 atataaagca tatgagggaa ttttaatttt ttgaaagata ataaattgat gtttaaaact
     9601 ttaagaagta caacacctag taatataata aatagcccaa catactcatg gtcataactg
     9661 atgtctgttc cataattaat tactaaaaaa atgatcaaca tttttaaaac tgcaattaaa
     9721 taattgtcct ttgttacaga caaatcatca acaaaaatat tgttattcct cgaaaacata
     9781 ttaattctcc taaaataaag tttgacgttt aatttttatg aaaattaagt taattataca
     9841 aggtttttta ttgttttaaa agctagtata ttattaattt gtattgcata tcgttttgtg
     9901 aataacatgt tgaggggcaa tcattgtgta ttatatcaac tagtattatc cccatatcag
     9961 aatgagtaat gaggataggt tatttataaa gtggattaaa ataacgactt ctaatcgctt
    10021 tgtttttaaa tatattaggc caaaaatcac tccagaatat aaataaggta agtacccaat
    10081 ccaagtatcc gattcatgca atgatgcaaa cactagacta gacacaacta atccaataaa
    10141 taaatgcttt ctaaatacaa ctcttattat catgcctcga aaaacgattt cttctacaat
    10201 ggcagggata atagcaattg tgacaataga catatacaaa ggcatatgtt gtaattctcg
    10261 atctaattct tgttcatttg tcggtacgtc atgaaaatgt aaataaagat gatctaaccc
    10321 ataaattagc aatgcaccac cgatgatata aatcacttgt gacattgtta attttttaaa
    10381 tgagagtaaa ttaattttga ataaccgtaa taacacaata ccgatgagca ctataaacac
    10441 gccataaagt atgtgtgtat cactgacaac gaaaccaatt agtagaataa tagtaatcaa
    10501 gaatgcggat aataaagtta gaaaataatt tccacgtgca actgataaat catcataaaa
    10561 caaatccttg aatttctgca tatttaatcc tttctatcat ctttaaaata aatattaaag
    10621 catttcattg gtatggtcat tttcattata tgagaaactt agatatgttt aaaggcgcat
    10681 cattacactt aactaaaacc atttgtttgc aatataaacg gtgtcaatta tacagtgtca
    10741 aattactaaa aataaaattg caaagttaat ttttgtagat ttgatgcatg tatttctgta
    10801 aaatatataa aatacacttt caattgacgc taatgtttaa aattacgtca atattataaa
    10861 ggagagaaat ccttaattat gaataaagca tcatttgata aaaaggttaa aaagcaatta
    10921 tggtttttga acaaaaatga aaaacaggca ttagatcaac ggttgtctag tattactgac
    10981 aaggacaatg tgaactttaa taagccaatc acctttgcta atacgtactt aagagaaaat
    11041 gtatttcgta gtaaagaaac taaaagttat agcatctttg taacattagt ggtgatgatg
    11101 tttgcatacg ttgctttatt aggtttgttt ttatttggat taattacaag tctatctggt
    11161 gtgcaatttt tcgttaaccc taaagtagaa ttgtcgacaa cggttgttat tttaacaatc
    11221 attggggcga ttttattaat gttagttagt atctatttaa taaaaatcac aacatcatat
    11281 tttacaaaga aactgttaga acataaattt aatagtcatt agtattttca atgaaatgct
    11341 tcaatgctaa actttcatag cattttttgg aaataagaga catgggttca tcgacttcta
    11401 atgttttgcc atttttgaaa tggagcatag tcgaattgcc catttccgtc ataccaatta
    11461 tatagtgtgc attgatatat gtttgtatcg gtgcacgctt ttgttttatt gggaaaagaa
    11521 tcgttttcgg attaaggtaa ataggcacca gcttgttaat gttgagtatg ctttttgccg
    11581 catcgattaa aattgatgct gatgtaaaat gcaatttagc ataataagga atggctttag
    11641 taatagggaa ttcgagattg tattcatggt acagaaagac acattttgtc tcaagatggt
    11701 tcgaagttgc ggtttggata taaagtaggt gttttgtaga aatgtcttgc aaatatagta
    11761 ctccttttca atcagttaat aataaaatgg ttcgcataaa tgaaatgcat attattgagc
    11821 gttccaagag tattaatcat tttaatcaac acgacacgtc gcatctattt aaaaaaacgt
    11881 tggcatttac gcttaactga tgccttaatt aatttaattg ttgaagttga taatgacata
    11941 atatcagcaa tttcatattg cttatagcct tcaagataaa gcataagcca ctgacgttcc
    12001 ctgtggttta gtagggcaca aacatcttgt aaacgaaggt catattcatt aaagtatgtt
    12061 ggctgttcag ttagtattgg tgaatgattt tcacataaaa tgatatcatt taaacgtttt
    12121 tgttgtctga atagatcgat aaggtaaaag tttagtcgca tgaataaaaa agaggataaa
    12181 gattgattgc ttgagggttt atatatctga ctcaattgcc acatttttat caagagtagt
    12241 tgataatact catcataatt atagctaata ttatattttt ttaaaagatg gtgtatgatt
    12301 ttgtggtgtt tgttgtatac gtcattaaat ttcaagtagt cattctccaa gttatacgta
    12361 taacaaatat ttccggataa agatagaata aaatatttag aaaaatcatt catttgcgta
    12421 atcgctaaat taagtgttaa atataagaca taagtaatta atttaatgtg atatgatgta
    12481 ttattacttt gctaaatagt agatagaaca aaatttgtaa tcgggaggta acaatggatt
    12541 acgcacattt aaatttagaa catttttttg cacgaaacga cgatttagat gttataagag
    12601 atcgcgctga tttcgtgatg ataaataact tcactaatga aatgatgtat cgtgatggtc
    12661 aaattgaagg tacaattgat ttaaatcagt actattataa aaatagatca aatgcagcaa
    12721 gttttattat gatggattat aaaaaagaaa ctaagtaaac gaacaagaga attttttgtt
    12781 ttttaatacg tgaataataa gattattgat ataaaggttt tcaaaggtta tacaaaaaga
    12841 taaaacattt atgattcgtg aatcaacgta aagtaatgtt gataaatcgt ttaaaacgtt
    12901 tcatttacat tactgtttat ttatgaatat gtaacaatgc atagataaaa ttgttaaacg
    12961 gttttaataa tgttaaactt tattatcgta gtcaaactga atgtataaca acaatgacct
    13021 aagaggtgtg gatatgaata aacacaagaa aggttctatt tttggaataa taggacttgt
    13081 tgtcatattt gctgttgtct catttttatt tttctcaatg atatccgatc agatattttt
    13141 caaacatgtt aaatccgaca ttaagattga aaagttaaat gttacattaa acgatgcagc
    13201 aaagaaacaa ataaataatt atacgagtca acaggtatca aataaaaaga atgatgcatg
    13261 gagagatgca tctgcaactg aaattaaaag tgcaatggat agcggtactt ttatcgataa
    13321 tgaaaagcaa aaatatcaat ttttagattt atcaaagtat caagggattg ataaaaatag
    13381 aattaaacgt atgttagtcg atagaccaac gttattgaaa catacggatg atttcttaaa
    13441 agctgctaaa gataagcacg ttaacgaagt ttatttaatt tcacatgcat tattagaaac
    13501 tggcgcagtt aaaagtgaat tagctaatgg tgtcgaaatt gatggtaaaa aatactacaa
    13561 tttctatgga gtgggagctc ttgataaaga cccaattaaa acaggtgcag aatatgctaa
    13621 aaagcatggt tgggatacac ctgaaaaagc tatttcaggc ggtgctgatt tcattcataa
    13681 gcacttctta tcaagcacag atcaaaatac attgtatagt atgagatgga atccaaaaaa
    13741 tccaggagaa catcaatatg ctacagatat taagtgggca gaaagtaatg caacaattat
    13801 cgctgacttt tataagaaca tgaagactga aggaaaatac ttcaaatact ttgtgtataa
    13861 agatgacagt aaacatttga ataagtaatt tgttagagtt aagttatgat atgacttgct
    13921 caagtacaaa aagacgcttt ctatcgcaat aaatagaaag cgtcttttta ttacttaaaa
    13981 tagtataagt gttagatata atcctaaaag tgtgaaaaat agtactggaa ttgtaatgat
    14041 gataccagtt ttaaagtatg tgccccaaga aatcttaaca tctttttgtg ttaagacgtg
    14101 taaccacagt aatgtagcta aagagcctat cggtgtaatt tttggaccta aatcagaacc
    14161 gataacattt gcataaatta ggccttcttt taacatgcca tggacatttg attgaccaat
    14221 agcaatcgca tctattaaaa ctgtaggcat attattcatt attgatgata aaaatgctga
    14281 aatgaagccc attcccaaaa tagtgctaaa tagaccgtaa ttggaaatat attctaatat
    14341 tttagctaat attaaagtaa tgccagcatt tcttaagccg aatacgacga tatacatacc
    14401 aattgaaaat aatactatat tccaaggtgc gcccttaatg acttgcttaa tatttacagc
    14461 atttgattta cgagccaata ttagaaatat aaaagcaatg attccggtga aaattgatac
    14521 cggaatttta gtaaattcac tgattagata gccgaaaagt aatataacta gaacaatcca
    14581 tgaaatttta aatagcttta aatcattaat ggcatctata ggttttttta tgtgatttgc
    14641 atcaaatgat ttaggaatcg cttttctaaa atataaccac aaaactagaa ggctagctaa
    14701 taacgagaat aaattaggga taatcattct actaagatat tgagagaaac taatgttaaa
    14761 gtaatctgca gaaatgatat tgaccaaatt gctcacgatt aaaggtaaag acgttgtatc
    14821 agcaataaaa ccacttgcaa taataaatgg aaatatggcc cgcttactaa aacctatatt
    14881 ttttaccatt gctaatacaa tgggcgttaa gattaaagct gcgccatcat ttgcgaaaaa
    14941 tgcagcaacc acggcaccca ataatatgat ataaacgaac atttttaggc cactaccttt
    15001 tgaagcatga agcatgtgaa tagctgacca ttcgaataat ccaactttat ctaatattaa
    15061 tgaaataaga atgactgata caaaagttaa agtggcattc cagactatac ctgttacttc
    15121 gaaaacatcg gaaaaactta caacaccagt aataacagca ataaaggcac ctgctaatgc
    15181 agtaaatcca atatctaggc ctttaggttg ccataataca aataataaag ttactagaaa
    15241 aatgaatgtc gctaaagttg tcatcattag cattcaccag tcttaaggtt atgacaaata
    15301 catcgttggt tagaggtatg aacttttgac aagttattaa ttacggactc aaaaatatta
    15361 tgattaagct ggtataaatg tttatttccg atttttcgtg tcgtaactaa gttggttttt
    15421 actaatgctt tcatatgata gctaagtgtg ggttgagaga attgaaaatg ttctaacaaa
    15481 tcacaagcgc ataactctcc acaagaaagt aaatctagta tttctaatct gcttgaatct
    15541 gataaaattt ttaaatatgt tgctagttct ttatacgtca taacatacct cctagacgtt
    15601 aaatagatta tcatctatat agataaatat ctatgtttct ttggtatatt tcacgatatg
    15661 actatgtaat ttaaatttgg ttttagtatt aaaagggtat taaagataaa ttataaatat
    15721 tgattttgca aaatatactc tttattctgc attgaaaaag gtgattagcc atatcttgag
    15781 tgtatggcca atcaccttag ttgaatttat tttgtaatca agggtattga ttaagaagct
    15841 ttatttttac gactaaaaat cataaataga attactaatg agattaatat ctttgctata
    15901 aatttcaatt taattcacct cgttaaaaat attttaacac ataagctgtt gtcgatattg
    15961 aatgaacatc tttaaaacga atcataatta tgatgaagta atgaagtatg acaatggtga
    16021 attcataaaa tatggcaaat tgttaaaaat attttaacac aaacactaga tttgatatta
    16081 aatgaaaagt tttaaatggt ttagtggttg taaaagtgaa ataatagatt accttaataa
    16141 attttattaa aaataccaaa ttgctataaa tatgggtaat agataaaaga aaatatttaa
    16201 aatgactaaa ggatggatga tatatgcgtg tagcaataat cggcatggga actgctggtg
    16261 taagtgtatt gagagagtta gttaagcacc cgaaatttaa tcaattagat atagatttat
    16321 acgatgataa agtaaatatg ggacagggcg taccatttca aaatgatagt tctgagctgc
    16381 taattaatat gccttcgaaa aagatgagtt taaatttaga tgatgagact gaattttgga
    16441 agtggtataa acaacaaact gattttaatt ttgatgaacc tgcatattta ccgagatttg
    16501 tattcggaca ttatatgaaa tcttatttat ctatgtatac gaaaaaatat ccaaatatat
    16561 caactaatta taataaagtt caagagattt atacaaattc taatatcgat gaaacgaatt
    16621 taacttatta catatgtact acaaattcag aacaatcatg gcaaacgtac gattatgtat
    16681 ttttaacatg tggtacattt gcctaccatg atccatataa cttgaagggc aaaaaaggat
    16741 atatcgctac gccttatcca acatataata cgttagacga agtaaatgaa ctggacgata
    16801 ttgcgattat tggtacaggc cttgcaagtt tagatgttgt tcgttatgtt gctgctcatc
    16861 atccgaaatt accaattact atgacgagtc gttctgcaaa tttaccaagt gtaagaggta
    16921 caatgttaga cgtgacattc aagtacttaa ctaaagacaa attaaatgac ataaaaaaac
    16981 atcatttcgg caatgcaccg cttgatacgt tagtttcttt atttttaaaa gaatgtgctg
    17041 aatatgacat tgattttgaa aaattagtac atagacgtac aggaaatcat attgcagatt
    17101 tgaagtatga tttagcgcgt ccaacagaaa tgggcatttt ccaaagtatc attgaatatt
    17161 tgaaagagaa tttaaattgg atatggaata gcttaagcat tgaagaccaa catcaattta
    17221 atcaaaaata ctcaaagatg attcaattaa attcaaatcc aatgcctcca agaactgctg
    17281 aactaattat tgaattaata gaaaataagt ctttaatttt gaaaaaagat ttagaagatg
    17341 tgaaacatga tggcaagttg tattattttt catacaaaaa ccaagaatca gtagacatat
    17401 ataatgttgt cattaacgcg acaggtgcaa aaagtcattt aaatgaatta gatcaagatg
    17461 atcaattaat taaaaatctt gaaaatagac aaattgtaca agcacatcca atgggaggca
    17521 tacaaatcat accggaaacc aatcaagtta ttagtccaag gtttggaaca ttaacaaata
    17581 tgattgcgat aggtcaaatg acaaacggtg ttaataaact acgaaatggt gtgaaaatga
    17641 tagtagaaca agttgcgcat acagtatctc aattatatga tgctttagaa tcgaatgagc
    17701 aagaacagcg aagtgacaat caataatttg taactagaag ataataaaga gaacgctcta
    17761 tagagacgca ttgaggtttt gattttaatg tctgttagta agaatcatag caatgagatg
    17821 cctatagtac tcagattata ttaaattaaa accgtcatta attgtttttt cagaaaacat
    17881 atagtatcat tttaaatgta gttgacatac tacgtactca aataatctat aacaatttca
    17941 tatataattc tttcggggca gggtgaaatt cccaaccggc agtaaattaa gcctgcgacc
    18001 tgctaatatg tttcgtatta gtggctgatc tagtgagact ctagagccga cagttaaagt
    18061 ctggatggga gaaagaatgt taattatcga caaagataat gtagcgtatt tgtaaaaatg
    18121 tgtacaaata ggcttattta acgataaatt tttctccttg catcttaatt catgatgtga
    18181 ggatttttgt ttatagaggt gatcatttga gtcaatttat ggattatgcg attcaacttg
    18241 caaatatggt acaaggtcaa acaggtgtta atccacccgt tggcgctgtt gtagttaaag
    18301 aaggtaggat tgttggtatt ggtgcacact tgagaaaagg tgacaagcat gcggaggttc
    18361 aagcacttga tatggcacaa caaaatgctg aaggtgcgac gatttatatt acgttagagc
    18421 catgtagtca ttttggttca acgccaccct gtgttaacaa aataattgat tgtaagatag
    18481 ctaaagtagt ttacgcaaca aaagacaatt cgttagatac acatggtgat gagacgatac
    18541 gggatcacgg tattgaggtt gaatgcgttg atgatgaacg tgcatcacaa ttataccaag
    18601 acttttttaa agcaaaagca aagcaactgc cacaaattac agtgaaagta tctgcaagtt
    18661 tagatggtaa acaagcgaat gataatggac aaagtcaatg gattactaac aaagaggtta
    18721 aacaagatgt ctataagtta agacatcgac acgatgcagt gttaactgga agacatacag
    18781 ttgaattaga tgatccacaa tatactacac gtattcaaga tggaaaaaac cctataaaag
    18841 taatattgtc taagtctggg aatattcatt ttaatcagca aatttatcaa gatgaatcaa
    18901 caccaatttg gatatatact gaaaatccaa atttaacaac caatcaaaca catattgaaa
    18961 ttatttactt gaagtcttgt gatttaacaa caattcttca caatttatat aaaagaggag
    19021 ttggaacttt gctagtcgag gcaggtccaa ccactacttc agaatttctc caatctaatt
    19081 atatagacaa atttattctc tattatgccc cgaaattaat tggcggatct ggaaattatc
    19141 aattttatca aacaaatgat gtgattgaga taccagatgc gaaccaattt gaaattgttc
    19201 attccgagtt attaaatcaa aatgtcaaat taactttacg aaagaagtga tgatgcatgt
    19261 ttactggcat cgttgaagaa ataggtgtcg ttaaaagtgt tcaaattcgt caatcagtca
    19321 ggacgattga aatcgaagca cataagatta cggcagatat gcatattggt gattccatca
    19381 gtgttaatgg tgcatgttta acagtgattg attttaatca gacatctttt actgtacaag
    19441 taattaaagg cactgaaaat aaaacctatt tagcagatgt taagcgacaa tcagaagtaa
    19501 atttagagcg tgccatgagt ggtaacggta ggtttggtgg acattttgtc ctcggtcatg
    19561 tagatgaact aggaacagtt tcaaaaataa atgaaacagc caatgccaaa attattacga
    19621 ttcaatgtag ccaacatatt aataagcagt tagtgaaaca agggtctatc actgtggatg
    19681 gtgtaagtct aacggtattt gacaaacatg ataacagttt tgacattcat cttattccag
    19741 aaacgaggcg ttcaacgatt ttatcatcca aaaaattagg agataaagta catttagaaa
    19801 cagacgtttt gtttaaatat gttgaaaata ttttaaataa ggataaagac caattatctg
    19861 tagataaatt aagagcattt ggtttttagg aggggtagca tgcaattcga taatattgac
    19921 agtgctttaa tggctttaaa aaatggagaa acaattattg tagtagatga tgagaatcgt
    19981 gaaaatgaag gtgatttagt agcggtcact gaatggatga acgataatac cattaatttt
    20041 atggcgaaag aagcaagggg attaatatgc gcaccagtgt ctaaagatat tgcacaacgt
    20101 ttggatttgg tacaaatggt tgatgataac tccgacatct ttggtacgca atttacagtg
    20161 agtattgatc atgtagatac aacaacagga attagcgctt atgaacgtac attgactgcc
    20221 aaaaagctca ttgatccaag tagtgaagct aaagatttta atcgtcctgg tcatttattt
    20281 ccattagtag cacaagataa aggggtatta gctcgtaatg gtcatactga agcggctgtt
    20341 gatttagcta aacttactgg tgccaagccc gctggtgtca tttgtgagat tatgaatgat
    20401 gacggcacga tggcgaaagg acaagattta caaaagttta aagaaaaaca taaattaaag
    20461 atgattacga ttgatgattt aattgaatat cgtaaaaaat tagaaccaga aattgaattt
    20521 aaggcaaaag tgaaaatgcc tacagatttc ggaacatttg atatgtatgg ttttaaagcg
    20581 acttacacag atgaagagat agttgtactg acaaaaggtg caattcgaca acatgaaaat
    20641 gtacgcttac attctgcgtg ccttacaggc gatattttcc atagtcaacg ttgtgattgt
    20701 ggtgctcaac ttgaatcgtc tatgaagtat atcaatgaac atggcggcat gattatttat
    20761 ctacctcaag aaggtcgtgg cataggattg ttaaacaaat tacgcgcata tgaattaatt
    20821 gagcaaggat atgatacagt aactgcaaat ttagcattag gttttgatga agatttgcga
    20881 gattatcata ttgctgcaca gattttaaaa tattttaaca tcgaacatat caatttatta
    20941 agtaataatc caagtaaatt cgaaggatta aaacaatatg gcattgatat tgcagaaaga
    21001 attgaagtta tcgtaccaga aacggtacat aatcatgatt atatggaaac gaaaaaaata
    21061 aaaatgggtc atttaatata ggaggacttt aacatgaatt ttgaaggtaa attaattgga
    21121 aaagaattga aagttgtaat cgtagttagt cgatttaatg attttatcac tggaagatta
    21181 cttgaaggtg caaaagatac tttgattcga catgatgtta atgaagacaa tattgatgtt
    21241 gcatttgttc cgggtgcgtt tgaaattcct ttagtagcta aaaaattagc ctcatcagga
    21301 aattatgatg cagtaattac attaggatgc gtaattcgcg gtgctacgtc tcattttgat
    21361 tatgtttgta atgaagtcgc gaaaggtgtt tctaaagtaa atgatcaaac taatgtacca
    21421 gtcatatttg gtattttaac gactgaaagt attgaacaag ctgttgaaag agcaggtacg
    21481 aaagctggca ataaaggtgc cgaagcagca gtaagtgcaa ttgaaatggc taatttatta
    21541 aaatctataa aagcatagag cgctgttgtg agttcataat caaagattta taaatgattc
    21601 agaactgcca atataatatt taggagccta gaacactgat taatgttcca gactcctaaa
    21661 ttattgttta tgccaagctc tatttacggc ataatttttt aattgtactt aaacataaca
    21721 taactgtagc tcctaaagct gcaattatgc caacacgttt taagccagca ggtttcacaa
    21781 attcttttgc agcaagagaa aggttttgtg ggcgttctgc taatcttctc ataaaatacg
    21841 caaaccaatc atcgccataa ggtacataaa tagtgaaatt atatccttca tttgcaattt
    21901 cttctgctaa ctctgatcta aaaccataga gcatttggaa ttccatacga tctttttcaa
    21961 tgtgattttc tttcataaat tgttttacat gattaatgat gcgatggtca tgtgttgcaa
    22021 ttgaagtgaa attgcgtgcg tttaacaaac gttgttcaat tatcttgatg taatttgcat
    22081 ctacgtcttc cttagattga aatgcaattg attcgttttc tttatatgca cctttaacca
    22141 aacgtaatcg taaatcttgg tacttatcaa ctaattcatg gctatcgtat aaatatgctt
    22201 gaattacagt accaacattt ctaaattcgc cttttaatcg atctaaaact tgaacaattt
    22261 gttgcaggct agcatatttt tcagtatcaa tatttatatg catattattg taagtatttg
    22321 ctttaagtaa aatctctctt aaattttggt aagctaattc taagtcgaat tctgcaccta
    22381 actgactcaa tttaacagac atatgtgcct ttacgccatg ttgatgaagc gcgtccataa
    22441 ttgttaaaat ttgttcttta gcatgattac tttcttcaac tgtaccgaca aattccccta
    22501 aattgtctac cgtaacagcg atattcttgt catttaagta ttcgattgta ttaattaact
    22561 ctggaattgt atttccggca acgactttat tggcacccaa acgtgggccc actttttttg
    22621 ctgcgttgtt taaaaaacta ttattagata atccgataaa aaaattcttt aatagtgcca
    22681 ttgctactcc tccaaaccct tgtgtttaca ataagtttag catgaaaaaa atattcgaga
    22741 aagcgttttc atcaaaaaca attaaaaata attatgtata attacactat tattgatgtt
    22801 gatttcattt gtatataata atatggacaa caataatact ataaatagaa atctacatag
    22861 tgaatttgct ataattatgt tattgaagat taatgaaaaa ggggcagtat aataatgtgg
    22921 aagtgggaag ctgaaaatga tgcgaaaggc gtcgttgtta ttgctcataa tatattagaa
    22981 catacgggaa gatatgcata tgttattacg atgttaagaa gaaatggcta tcatgtgatt
    23041 atgggagatt tgcctggtca aggtcaaact tcaagagcta ataaaggaca aattgaaaac
    23101 tttcaaacgt atcacgaaag tttgctagac tggttaaaga tagctaatga atataaaatt
    23161 cctacttatg tattaggggt aggacttggc ggtttgattt tattaaattt acttgaaaaa
    23221 gtagaattgc caatcgaggg gatgatgtta atttctccta tgctagaact tcaaaagaat
    23281 ggaaaaaatc gtaaagataa acttgtttca aatataggta aaatttcaaa agacacacgt
    23341 tttaatgttg gtgtagaacc aaaagattta acacgtaatt tagaaattgt tgaagaaaca
    23401 gttaacgatg gtttaatgtt aaaaaa
//
LOCUS       DAMZPP010000026        22818 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMZPP010000026 DAMZPP010000000
VERSION     DAMZPP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22818
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.026"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(126..1178)
                     /gene="icaC"
                     /locus_tag="POH73_000796"
     CDS             complement(126..1178)
                     /gene="icaC"
                     /locus_tag="POH73_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HDI6886771.1"
                     /translation="MKKIRLELVYLRAIICAIIIVTHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDTSFQKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
     gene            complement(1165..2037)
                     /gene="icaB"
                     /locus_tag="POH73_000797"
     CDS             complement(1165..2037)
                     /gene="icaB"
                     /locus_tag="POH73_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HDI6886772.1"
                     /translation="MKYRKLIILVLSILIILPVSTLDGHHIANADDDPPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDMHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            complement(2034..2339)
                     /gene="icaD"
                     /locus_tag="POH73_000798"
     CDS             complement(2034..2339)
                     /gene="icaD"
                     /locus_tag="POH73_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HDI6886773.1"
                     /translation="MVKPRQREYPTLKSSLNIIRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQKGRES"
     gene            complement(2303..3541)
                     /gene="pgaC"
                     /locus_tag="POH73_000799"
     CDS             complement(2303..3541)
                     /gene="pgaC"
                     /locus_tag="POH73_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDI6886774.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELESITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            3705..4265
                     /gene="icaR"
                     /locus_tag="POH73_000800"
     CDS             3705..4265
                     /gene="icaR"
                     /locus_tag="POH73_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HDI6886775.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            5111..5773
                     /locus_tag="POH73_000801"
     CDS             5111..5773
                     /locus_tag="POH73_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HDI6886776.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNRISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
     gene            5790..6482
                     /locus_tag="POH73_000802"
     CDS             5790..6482
                     /locus_tag="POH73_000802"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HDI6886777.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIEDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNKDEVKKGKELIEATGAKLLGVVLNRMHKDKS
                     ASYYAYYGTDES"
     gene            6479..7246
                     /locus_tag="POH73_000803"
     CDS             6479..7246
                     /locus_tag="POH73_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDI6886778.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILQGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            7481..7981
                     /locus_tag="POH73_000804"
     CDS             7481..7981
                     /locus_tag="POH73_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6886779.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATPEALKLRIVQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVPANNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFENLAKNQHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            7984..8460
                     /locus_tag="POH73_000805"
     CDS             7984..8460
                     /locus_tag="POH73_000805"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HDI6886780.1"
                     /translation="MAVVYVAGGCLWGVEAFFTTIPGIIHTEAGRANGRSSKLDSPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNRQGNDIGEKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDKIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISIDLLNKYK"
     gene            8539..9150
                     /locus_tag="POH73_000806"
     CDS             8539..9150
                     /locus_tag="POH73_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDI6886781.1"
                     /translation="MHTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGNFKINPHALQAVSRLAGNDYAKLGGIFTIDRPEK"
     gene            complement(9235..9594)
                     /locus_tag="POH73_000807"
     CDS             complement(9235..9594)
                     /locus_tag="POH73_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886782.1"
                     /translation="MILRYVTNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSAAAYKHFEAGQGKKGAQHALDL
                     LGLATLSLLDTFSCNKK"
     gene            complement(9744..10046)
                     /locus_tag="POH73_000808"
     CDS             complement(9744..10046)
                     /locus_tag="POH73_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886783.1"
                     /translation="MRTNNHITHLDAIELSSGWNYNSKLVAGKNALLSDSLLRDIKVL
                     SSNAKGDADMLETELHAVINAMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            complement(10033..10976)
                     /locus_tag="POH73_000809"
                     /pseudo
     CDS             complement(10033..10976)
                     /locus_tag="POH73_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11290..11982
                     /locus_tag="POH73_000810"
     CDS             11290..11982
                     /locus_tag="POH73_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDI6886784.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFEYAGVTKEACIEAPGYRVKESPVHFECEYVQTIRIPTGDTVSTVDIVI
                     GRVAQVHIDDKFILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPSASKEELAGLE
                     GRNFDNQSDNKE"
     gene            12529..16584
                     /gene="sasA"
                     /locus_tag="POH73_000811"
     CDS             12529..16584
                     /gene="sasA"
                     /locus_tag="POH73_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HDI6886785.1"
                     /translation="MSKRQKEFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKITGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTTDSTSVTKNSSSEQTSNSDTVSSEKSENVTSTTNSTSNQ
                     QEKLTSTSESTSPKNTTSSSDTKSVTSISSTDQQTNTSTNQSTASNTTSQSTTPASAN
                     LNKTSTTSTSTAPIKLRTFSRLAMSTFASAATTSAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGNGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNAAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSAADNAAKLKVQPTNNMFQDFDINYNGDTKVMTVTYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTITIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSGSVSKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSVSMSTSGSLSKSQSLSTSTSDSASTSQSVSDS
                     TSNSISTSESLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSDSVSGSLSIATSQSVSTSSSDSMSTSEMISDSMSTSGSLAASDSKSMS
                     VSSSMSTSQSGSTSESLSDSISTSDSDSKSLSLSTSQSGSTSTSTSTSSSVRTSESQS
                     TSGSMSASQFDSTSISTSFSDSTSDSKSASTASSESISQSVSTSTSGSTSGSTSTSTS
                     ESLSMSGSDSTSVSDSTSMSESDSTSVSMSQDKSDSTSISDSESVSTSTSMSLSTSDS
                     TSTSESLSTSMSGSQSISDSTSTSMSNSTSMSNSTSTSMSGSTSTSESNSMHPSDSMS
                     MHHTHSTSTSRSSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNG
                     LLGGVMTLLVGLGLMKRKKKKDENDQDDSQA"
     gene            16713..17924
                     /gene="secY2"
                     /locus_tag="POH73_000812"
     CDS             16713..17924
                     /gene="secY2"
                     /locus_tag="POH73_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HDI6886786.1"
                     /translation="MLKLLQQYEYKIIYKRILYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLITLVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMRSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKDVSDDMPMMTF
                     DSPIGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGSALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            17935..19488
                     /gene="asp1"
                     /locus_tag="POH73_000813"
                     /pseudo
     CDS             17935..19488
                     /gene="asp1"
                     /locus_tag="POH73_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
     gene            19478..21046
                     /gene="asp2"
                     /locus_tag="POH73_000814"
     CDS             19478..21046
                     /gene="asp2"
                     /locus_tag="POH73_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HDI6886787.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIILT
                     DDMLDSPLEIPTKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIEETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            21024..21983
                     /gene="asp3"
                     /locus_tag="POH73_000815"
     CDS             21024..21983
                     /gene="asp3"
                     /locus_tag="POH73_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HDI6886788.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQDNLEYKSMRVTKIG
                     DDQYGNDRINVIIAEPSDAYPTISNDFLKSFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLDCKVYRTSHEDDDLKEWFTEHVPGNHELKDT
                     NVEVYFKEEQDNHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            21973..>22818
                     /locus_tag="POH73_000816"
     CDS             21973..>22818
                     /locus_tag="POH73_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6886789.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDVLKQKTL
                     EFKERIASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDL"
BASE COUNT         8107 a         3456 c         3983 g         7272 t
ORIGIN      
        1 tggcatttac gtagtttaaa tgtgcaatta gaatatatgt ataacaatat taaacacgcg
       61 gtaaaacgaa gtcagtcaat tcaaactgat ttcgcccacc gcgtgttttt aacatagctt
      121 aataattaat aagcattaat gttcaattta tatggttgtt ttccaataat aaacctaaag
      181 atatagaatt cacgcaatat cataccgaca cctatacata atcctaaaat gaatagtagt
      241 gatatcgcta gaaagaccat tgtattatcc tcaaatatat ttgtatatgc aaacaatgag
      301 tctagaatga ttggatgtaa taaataaata aagaatgaga aagcactaat catttgaatc
      361 gtattaaata acattgtttt aaaatgcgtg caaataccca agataacaat aaacataata
      421 ctattatatg gtgttaatga atatgaaaag ctggtaacgt tccaatagtc tccatttgct
      481 aacgcaataa acacaaaata agtagctaca gctaatacaa tcataataac taaataacgt
      541 tctaagaaat ttaaaacacg ttcgtagtta taacccatat atgcacctaa aaagaaataa
      601 aaaatccatc cgaatattat agtgttttca cttaatggat aatagtgtag cacggtatcg
      661 tgaaacgctg tgttgttcgt aaagtaatat aaaaatgatt gctgtaaaat aaaagataac
      721 aataataata ttttactgtt gaataggtta tagttaattt taaaaatgat ataactcaaa
      781 ataaagaatt gcatgataac aacgataaaa tagccatacc attgacctaa taggacattt
      841 tcaataaatt gtttttgaaa acttgtgtca gttaataatg attcactata actgtaaaac
      901 aaacccatta atatgtaagg aatcaatata tattttacgc gtgtagttaa gtatctatag
      961 gtgacttttt ggtaattcaa ggttgtcagt aactgtgaca agataataaa gcaaggtgta
     1021 ccaaaaatga caatattacg aatatagaac tgtagtacta aagatcctcc ttccatattc
     1081 tcatgtttta aagtaatttg tgttaataaa tgtgtaacaa taataattgc acatataatg
     1141 gcacgtaaat atacgagctc aagtctaatc tttttcatgg aatccgtccc atctcttaat
     1201 taaatgctca aaagcatcat cactaattaa tattctaggg atgtaataat cattggagtt
     1261 cggagtgact gctttttcct ctaatgaaaa accgtatttt aacccagctt ttttgattac
     1321 cggtaattta tcgtcattca tcaagccata aggataggct atagttttct gcgacttttt
     1381 aaagttttta gttagatatt tttcactttt gtttaaatct tttatgattg tagcttcaga
     1441 agctttcatt aattttgact tattattttt agataagtta tgcatatcgt gggtatgtgt
     1501 ttcaaattcc cataacccag ttttatacat ttcttttagt tcttttttac taatcatatc
     1561 gaggttgtga aagttttctt ccccaacatg acctgtgata ataaacccag ttgccggtat
     1621 tttatatttt tttaagattg gataagcatt ttcataaata gtttcatcca tatcatcaaa
     1681 gttaatccat acacttcgtt ttggaaactt accttttttc ttgtaatata aaaattcttt
     1741 caaagttaaa aatttagcat catgtgattt tagccatttt atttgagatt caaattgtga
     1801 ttggctaaca ctataatttt taatttcttt actactagaa aagaagtaaa tgaaattatt
     1861 cagaaaattc gcttttctta cacggtgata atttaatgcc agagcactat tttctttata
     1921 tttcagtttt ttaggtggat cgtcatctgc atttgcaata tgatgaccat ccagtgtgct
     1981 tacaggcaat atgatcaaga tactcaacac taaaattatt aattttctat acttcacgat
     2041 tctcttcctt tctgccattt ttgaatcaat atgctaattg taaaaaaaac aaaaatgata
     2101 atcgcgaaaa tgcccatagt ttcaaatata tctaaaattt cagtattttc aatgtttaaa
     2161 gcaacacgta ttgtattgat actttcgtca tgaatttcaa atatagtacc aatataaacg
     2221 agtagaacaa ctaaacaata tatccaaaag acacacgata tagcgataag tgctgtttct
     2281 cttataatgt ttagcgatga ttttagcgtt gggtattccc tctgtctggg cttgaccatg
     2341 ttgcgtaacc acctttctta cgttttaatg cttttggaaa tgcgacaaga actactgctg
     2401 cgttaataat ccagtatact gtcggatacc aacttacaaa tatgagtcca gccatattct
     2461 ttttctcgta gcgactatca ataaagagtg cgactgtaaa ttgaataacg tttataaaag
     2521 tcatagtaaa tgatgatagt agaaatattg aaaaactata tgtcataaat gtatagtcta
     2581 agaagtttgc tgttatgaac aaatagccta aatatagaag cactatatat acccataaaa
     2641 ttgagatgat ttgctcaaac atcaaaatat ataaaggaaa ccttttcgtt ttcattgtgc
     2701 taaaaaagtc tcgtagtaat acttcgtgtc ccccttgagc ccatctcacg cgttgcttcc
     2761 aaagacctcc caatgtttct ggaaccaaca tccaacacat ggcaagcggt tcatacttaa
     2821 tacgatatcc acgtaaatgc aatttccaag aaactgcaat atcttcggta atcatatcag
     2881 tatcccagta gccaacgtcg acaactgcac tttttttaaa tagagtgaag acacccgaaa
     2941 tagtattgac tgcgccagca agtgtctgac ttcgcttaat acagccaatt aaacttgcat
     3001 attctatcgt ttgaatttta cctaaaatag aactcttatt tcgaattcta ggattacctg
     3061 taactgcacc aagttttgga tcatgtttga aattttcaat catataatat ggtgcatctt
     3121 gatcaacgat agtatctgca tccaagcaca ttacataatc atatgaagcc tgtttaatgc
     3181 cttgattgag tgcattggct ttacctctgt tttcttgtaa atcgacgaaa ataaagtcat
     3241 tattttcttt aattttatag atgagttctg ctgtattatc tgaacttcca tcattaatga
     3301 taataatttc ttttttctcg tatttgagtg caagaacatt agacaacgta tcttcaatcg
     3361 tttcactttc gttataacag gcaagtaaaa atgtaatgct ttctaattca tccacattta
     3421 tgtcaggctt cttgttcaat gaatatctaa tttctctagt aaaatagaaa taaattgaac
     3481 cgacaatcca gtaaatagac ataaatacag gataaaaaag caaaaagtta aaaaattgca
     3541 atttctttac ctacctttcg ttagttaggt tgtaagccat atggtaattg atagtatttt
     3601 aatttgcaat agattgttgt tataattaaa cggaaatatt tgtaattgca acttaatttt
     3661 cctgtaacat agtgtgatta attttcagta gggggtttta aaaattgaag gataagatta
     3721 ttgataacgc aataacctta ttttcagaga aggggtatga cggtacaaca cttgatgata
     3781 tagctaaaag tgtaaatata aaaaaagcga gtttatatta ccattttgac tcgaaaaaaa
     3841 gtatttacga acaaagtgtt aaatgttgtt ttgattacct taataatatt attatgatga
     3901 atcaaaataa atcgaactat tcaattgatg ctttatatca attcttattt gagtttattt
     3961 tcgacatcga agaaaggtat attagaatgt atgttcaatt atctaatacg cctgaggaat
     4021 tttctggaaa tatttacgga caaatacaag atttaaatca atcattaagt aaagagatag
     4081 caaaattcta tgatgaatca aaaataaaga tgacaaaaga agactttcag aatttaatat
     4141 tgctgtttct tgaaagttgg tatttgaaag catccttttc gcaaaaattt ggagcagtgg
     4201 aagaaagtaa aagtcaattc aaagatgaag tgtattcgct actaaatata tttttgaaga
     4261 aataattttt gttactagtt tgtaataatt aacttacttt tgtaaaaaaa gacatgagat
     4321 tattttttta aatctatata aagttgacaa tacaactcga aattgagaat attaagatgt
     4381 atatgaattt tataaattaa atacaataca ttaatataaa tatcaattgt tgcaaaatac
     4441 gatttgttca atgatttgat aatattattc tttatatttg tgaatggtta agtttgtctt
     4501 tgaacatatt ataaaagtgt aatgttcccc tgaaaagaat aagttgtcat ctaattacag
     4561 gaaatccgca taaattagat gaaatggaaa gtaataatta ataatttatt gataagcgcc
     4621 tatgtgatgg taaatcatga cataggcgct ttttttataa gtttaaaatg taaataaaaa
     4681 ttatataaat tacccacatc tttttaaaag atgtgggctt tatcatcatt aacccaactc
     4741 acagtgacgg gttacgcaaa gtattgaatt accgagtacg ggcacgctcg gtgttgtaaa
     4801 gagcaaataa tcaagtaatg atgctgcttc tactcgatta taagaaagcc ataatagaga
     4861 cgatggtatc taggtttatt actgataggt ttggatattt gaagttgggc aatattatat
     4921 ctcaaacgaa aatataaata aattatacat aacgaccaag aatcatgata taattttaaa
     4981 cgataaaata tttatataaa aaattagaga aaatgtagtt gtgtattttt gtggtcgtta
     5041 aactagatat aattgtccga tttataaaac atacataatg aatacaatga ttgattatgt
     5101 ggaggaaacc atgaaagaaa agtttgattt agtaaaacta ttaaatattc taaagaagaa
     5161 tattaaatta ttgcttattt taccggcaat atgtcttgta gtaagtgcgg cattaacctt
     5221 ttttgttatg cctgacaaat atactgcttc tactcaaata ttagtaaaca tgaaaaagtc
     5281 ctcaagtgat ttagctttcc aaaatgttca aagtagtttg cagtctgtta atacatatac
     5341 ggaaattatc aaaagtccaa gaattctaga taaagtatct agagagtttg atggtcagta
     5401 ttcaacagct gagttgaatt cgtttttaaa agtaaccaat caaacaaact ctcaaattat
     5461 tactgtatca gttacaactg ggaataaatc tgaatctgac aaaattgtta atagaatatc
     5521 taaagttttt gcacatgaca tgcctaaaat tatgagtgtt gataatgtta caatactttc
     5581 ctcagcacat gataatgctg taaaagtatc tccaatagta tctgttaact tagtaatcag
     5641 tatcattgtt ggtattgttt tagcaatatt gattattttc ttaaaagaat tattagataa
     5701 gcgtattaag acggaagaag atgttgaatc gcaactagga ttacctattt taggttcaat
     5761 acaaaaattt taatttacga ggaattacca tgacgaatac acgaagaagt acatcaagtt
     5821 taattgtcca tgaacaacca aagtcaccta ttagcgagaa atttcgaggc ataagatcaa
     5881 atattatgtt tgcaaatcct gacagtgcag ttcaaagcat tgtaatcact tcagaggcac
     5941 caggcgcagg taagtctaca attgcagcaa atttagcagt tgcatatgca caagcaggat
     6001 ataaaacatt aatcgtagac ggggatatgc gtaaacctac gcagcactat atttttaatt
     6061 tgccaaataa tgaaggccta tcaagtttat tgctaaattg gtcaacttat caagacagta
     6121 ttatctcaac tgaaattgaa gatttagacg tcctgacgtc tggaccgatt ccgccaaatc
     6181 catcagagtt aatcacatca agagcatttg cgaatttgta tgacacatta ttgatgaatt
     6241 ataactttgt aattatcgat acgccaccag tgaacacagt tacagatgcg caattatttt
     6301 caaagtttac cggcaatgtt gtctacgtag ttaattcgga aaataataat aaagatgaag
     6361 ttaaaaaagg aaaagaactt attgaagcaa caggtgctaa attattaggt gtagtcttaa
     6421 atagaatgca taaagataaa agtgctagtt actatgcata ttatgggact gatgaatcat
     6481 gattgatatt cataaccata tattagttga tatagatgat ggtcctaaga caattgaaaa
     6541 gagtattgca ctattaaagc aagctaagga tgaaggtgta acaagtattg tagcgacacc
     6601 tcatcatttg catccgagat atgataatac atttcaacaa gtgcttgtga aattggcgga
     6661 gttaagaacg catccagaag ttcaagcatt ggatattaaa ttatttcccg ggcaagaaat
     6721 tagaattaca gattcgattc tgcaaggctt agataatggt agtattcaag gtattaatcg
     6781 ttcgaaatat ttactaattg aatttccaac gggcgaagta ccacattata caaaacaatt
     6841 gttttttgaa atacaatcga gaggctacat accaatcatt gcacatcctg aaagaaatag
     6901 aagtatcgcc aaaaatccag aaatattata tgaacttgtt gcaaatgggg cattgagtca
     6961 attaacatcg agttctttag ttggtggatt tggaaaaaac attcaaaagt tatcattaca
     7021 atttatagaa tgtaatttgg ctcattttgt agcttcagat gcacattcat gtgaccaaag
     7081 accatttttg atgcaagaat tatttcacaa tcataaattg aagaaatatt cgaatgacat
     7141 tgaagcatta cttcgaaatg ccagttcagt aatcaatgat aattttgttt atctagacag
     7201 accgactaag ccaggaaaag tgaagtcgtt tttgaaatgg ttttaactaa atatattttt
     7261 tcgaattaga ctggtataca tgtaccagtc tttttgagta tgcttaaaat gaattataat
     7321 tatgattatt tgctaattcg tataatacaa cttgtctaat tgattaaggt gaatggcttt
     7381 taataaaaag actcgttttt tatagtgaag gtagtttgaa aataatgaat ataatgatga
     7441 aggtattata tttgatatat aaggctaagg aagtgttttt atggtattaa gaaaagtaat
     7501 catgcaggac ttagatcaaa ttattgcact tgaaaatatt ggtttttcac cggaagaagc
     7561 tgcaacacca gaggcattga aattgagaat tgtacaaatt caagaaactt ttattgtagc
     7621 tgaaaagaat gatgaagtga ttggatatat caacggtcct gtcataaaag agcgttacat
     7681 tagcgatgat ttatttaaaa atgtccccgc aaataatagt gaaggtggtt atattagtgt
     7741 gttaggtctt gttgtagcac caaattatca aggtcagggt attgcaggtc gcttactaaa
     7801 ctattttgaa aatcttgcta agaatcaaca tcgacatggt gtaacgttaa catgtcgaga
     7861 atcattaatt tctttttatg aaaaatatgg ttatcgtaat gaaggtgtat cagaatcatg
     7921 tcatggcggt attaaatggt ataaccttgt taaaaatata aaaaaggatg atgaggtgta
     7981 aatatggcag ttgtttatgt agcaggaggg tgtttatggg gtgttgaagc atttttcaca
     8041 acaatacctg gaattataca tacagaagca ggaagagcaa atggaagaag ctctaaatta
     8101 gacagtccgt atgatggtta tgctgaatgt gtcaaacttc attttgatga tcgtatgtta
     8161 acaattacag acatcatgaa ttatttattt gaaattattg atccatacag tgtgaatcga
     8221 caaggaaatg atattggaga aaagtatcga acgggtttat atagttgtgt agatgaccac
     8281 ttaattgaag cgcgtcagtt cattgaacga cgtaaagaca gagataaaat tgcagtggaa
     8341 gtcttaccgc tgtctaacta cattaaaagt gctgaagaac atcagcaaca tttagaaaaa
     8401 tatccagaag atatgcatat gtgtcatatt tcaatagatt tgctaaataa atacaagtga
     8461 ttttcagagg agaatagatg tttaaattaa ccgaaatcgg agaaatacct agatatacta
     8521 agaaaagagg taggtattat gcatacgttt gatgcacaat cattaactgc tagagaaaac
     8581 tataaattat taattggttc aattattcca cgacctatag cttttgttac aacattaaat
     8641 caagatgcat cagtaaatgc agcaccattt agttttttta atatagttaa taatcatccg
     8701 ccaatgattg ctattgcagt tcaacgtgca ggaggtaaga gaaaagatac tgcattgaat
     8761 atagaacgga ctggagactt tgttgtgcat attacagacg aagataatgt tcaagatatt
     8821 aatgagactg cagcgccgtt agcatatggt gacagtgagt tgagtcgtac agaattatca
     8881 cttttaacat ctacaacgat taagacgcca ggaattaaag acgcgaaaat gagattcgaa
     8941 tgtaaactat cgcaaatgat attgcttgga gatgtattag atggggctga cttaataata
     9001 ggcgaaattg taacttatca tattgatgac agtatttatg agggtaattt taaaatcaat
     9061 ccacacgcat tacaggctgt ttctcgttta gccggaaatg attatgctaa attaggggga
     9121 atatttacaa tagatagacc tgaaaaataa caaaacacat gctatagcgg tgtaccgttt
     9181 atggattgta tcaaaagtac acaggtgcta aagtatgtgc ttctaaaatg aagactattt
     9241 tttattacaa gaaaatgtat ctagtaaact taaagtagca agacctaata aatctaatgc
     9301 atgttgtgca ccttttttac cttggccagc ttcgaaatgt ttgtaagcag ctgcacttaa
     9361 aatacctatt gttgatagcg aagcaaggcg agaaatgttt ttattgataa agctagctga
     9421 gtataaagca gcagtagttg cttctgcaat gccgacgtat tttacaagtt ctttttgcaa
     9481 accaaaagta tgttcaaaca gttcaatcat acccttatct tcttgcaatt taggtttact
     9541 ggcttggtat agctctttcg caagttttaa attggtcacg taacgtaaaa tcatattttt
     9601 attcctccca atatttgatt ttttgaaaaa cggttgtttt atcattttac ccgtttcttc
     9661 aaaaatgaat ctcttatgta acatgtgtag ttcaggaatg tttgctatgg aaatataatt
     9721 ctgttcactc aaaatgtatg aaattaatgt gtagttttat cgagttgctc ttttaatttg
     9781 gttagattgt tttttagaga agcggtacta tttttaagtg catcaacaga tttaccttcg
     9841 ttttgagaca ttgcgtttat aacagcatga agttccgttt ctaacatgtc ggcgtcacct
     9901 ttagcattag aacttaatac tttaatatct cttaaaaggc tatctgatag tagtgcattt
     9961 ttgccagcta ccaatttcga attgtaattc caaccgctac ttaactcaat agcatctaaa
    10021 tgggttatat gattatttgt tctcattata ctttcctccc tcgatggaaa ttacattagt
    10081 atccgatttt aaacggccat ttccatctac cattgacggg gaaacatcat ctttataaaa
    10141 ctgtacaaat tttgtgctag gatatgattg cataacgtca tttatagatt tttgtaggtt
    10201 ccaaacatag ttagcaacag tctctctgtt ctcaccttta tcgtaatagg caataaactc
    10261 ggacttcata tttttttgta tttgaggata tttattaatc aaagattgta attgttgatt
    10321 taatgcatct ccttttaagt gcataacagg tttgaattct tttttgaact tatccaaatc
    10381 atcgatactg tctgtgttga aattcaaaaa ttgtttagta acaccattga attcttgttt
    10441 cttttgaagt ttttcattca tggcataata accgtttttc tcaacttctg atttgaattt
    10501 taatcgagct tgttcaattt caggttcata acgcttatac atcatggctg caactgccgc
    10561 tgatgtttca tttatatgac caggtgtaaa tgttgtgata gctatgccat ttccagactc
    10621 aacaaatatc agtctaaatt gaaagggaac gtcataattg acagtaccga cgactactaa
    10681 accatctacc ttactttttc gctaaatcca gatttattag tataggcctc aactaaatta
    10741 attttacaat cataaccaaa tgtatctttc acatagtctt tatatttttt tatcgcttca
    10801 cctttatgtt ttttcagaaa tacttcattt ctttttttat gttcaccaat atatttacca
    10861 tcataatttt tgacgctaac cgtctttgga atatcttttg tttggttctt tgatatgaaa
    10921 gagcaaccgc ttattatgat tgatgtgcat aataagattc gcataatttt tctcatatct
    10981 tcattagctc ctttgtatta gctgtttttc agttgatagg caaattatac aaaaactaaa
    11041 ataatgttat tcaatattat tataagctaa tcttgattat attaactaat attaattaaa
    11101 ttaacaaaaa acatcattaa atattaattg cataaatcct tatctaaaat tttcgaatca
    11161 agaacacaaa ttataagcaa ccacgtcata tatagtttct tcttataaat aaattgatta
    11221 tttatattag acgcttatgt catgagagcg taaccttaaa tgtaattact aataaggggt
    11281 gaggatagaa tgaattattc gccgaagcaa ggtgtaagga gtcatggctt accacacgat
    11341 ccatttaaaa gtagtacagt accacgtcca atagggtgga tctctactgt atcgaaagat
    11401 gggaaagata atttagcacc ttatagtcag tatcaaaact taacatggga tccgcctatg
    11461 gttatgttcg cagcaaatca atctgttctt ggtgatcatg agcgtaaaga tacagtaaaa
    11521 aatgcagaag agacagggtg gtttgtatgg aacatggcca cgtatgattt aagagaagct
    11581 gttaatttat cgtcgaaggc actaccacct gaagaggatg aatttgaata tgcaggtgtg
    11641 acgaaagaag catgtataga agcaccaggt tatcgagtca aagaatcgcc agtacatttt
    11701 gaatgtgagt acgttcaaac gattcgtatt ccgacaggag acactgtatc aaccgttgat
    11761 attgttatag gaagagttgc tcaggttcat attgacgata agttcattct tgataatggt
    11821 aaattagaca ttaagtcgat taaacctatt gctaggttag gatattatga ttacacggtt
    11881 gtgaatgaaa tatttgaaat gaaagcacca tcagcttcta aagaagagct tgcaggttta
    11941 gaaggaagaa attttgataa tcaatcagat aacaaggaat agtgaattca atcattttca
    12001 atacatcgaa actacacatt gaaattagtt tcgatgtatt tttatatttc aaaaccggga
    12061 agcgatatgt cgctatattt ataatttcaa agaagaattt tttagaaatt cagatatata
    12121 acactaggtt tattgaaaat atattgatat tactttaata ttaaagatgt aatataaata
    12181 taatatttat cttttagttg taaattgact attaatgaat aaaaggtgga tgataaatac
    12241 attttaataa aaagacattt ttactaaata agtaaaagtt atctaaattg ataaacattg
    12301 atttcaaaat tatataaaca taataaggca tgttattcag aatatagtta aaatattata
    12361 ctaaatccca cacaataaag aaagcgattt catcaaagaa tatacttttg agaaaaaatg
    12421 gattttgaca aattaagtat tttttatttg aaaaaagagt tgtattaaat tgaatagtgg
    12481 catatattat aatctaatga atgtaaagat aatacaagga gttattacat gagtaaaaga
    12541 cagaaagaat ttcatgacag cttagcaaat gaaaagacaa gagtaagact ttataaatct
    12601 ggaaaaaatt gggtaaaatc cggaattaaa gaaattgaaa tgttcaaaat tatgggacta
    12661 ccatttatta gtcatagttt agtgagtcaa gataatcaaa gcattagtaa aaaaataacg
    12721 ggatacggac tgaaaactac agcggttatt ggtggtgcat tcacagtaaa catgttgcat
    12781 gaccagcaag cttttgcggc ttctgatgca cctttaacat ctgaattaaa cacgcaaagt
    12841 gaaaccgtag gcaatcaaaa ctcaacgaca atcgaagcat caacatcaac aaccgattct
    12901 actagcgtaa cgaaaaatag cagttcggaa caaacatcaa atagtgacac agtctcaagt
    12961 gaaaagtctg aaaatgtcac ttcgacaact aatagtacaa gcaatcaaca agaaaaattg
    13021 acatctacat ctgaatcaac atccccaaag aatactacat caagttctga tactaaatct
    13081 gtaacatcaa tttcaagtac agaccaacaa actaatacat caacaaatca aagtactgca
    13141 tcaaatacta cttcacaaag cacaacgcca gcttcagcca atttaaacaa aactagtaca
    13201 acgtcaacca gcacagcacc tatcaaactt cgaactttca gtcgattagc tatgtcaact
    13261 tttgcatcag ctgcaacgac aagtgcagta accgctaata cgattacggt taataaagat
    13321 aacctcaaac aatatatgac aacgtcaggt aatgctactt atgatcaaag taccggtatt
    13381 gtgacgttaa cgcaggatgc atacagccaa aaaggtgcta ttacattagg aacacgtatt
    13441 gactctaata aaagttttca tttttctgga aaagtaaatt taggtaataa atacgaaggt
    13501 aatggaaatg gtggagatgg tatcggcttt gccttttcac ctggtgtatt gggagaaaca
    13561 ggattaaatg gtgcggcagt aggtatcggt ggtttgagta atgcgtttgg cttcaaattg
    13621 gatacgtacc acaatacatc taaaccgaat gcagcagcaa aagcgaatgc agacccatct
    13681 aatgtagctg gtggtggtgc gtttggtgcc tttgttacaa cagatagtta tggcgttgcg
    13741 acaacgtaca catcaagttc agcagctgat aatgctgcaa agttaaaagt tcaacctacg
    13801 aataacatgt tccaagattt tgatattaat tataatggtg atacaaaagt tatgactgta
    13861 acgtatgcag gtcaaacatg gacacgtaat atttcagatt ggattgcaaa aagtggtacg
    13921 accaactttt cgttatcaat gacagcctca acaggtggcg cgacaaattt acaacaagta
    13981 caatttggaa cattcgaata tacagaatct gctgttacac aagtgagata cgttgatgta
    14041 acaacaggta aagatattat tcccccaaaa acatattcag gaaatgttga tcaagtcgtg
    14101 acaatcgata atcagcaatc tgcattgact gctaaaggat ataactacac atctgtcgat
    14161 agttcatatg cgtcaactta taatgataca aataaaactg taaaaatgac gaatgctgga
    14221 caatcagtga catattattt tactgatgta aaagcaccaa ctgtaactgt aggcaatcaa
    14281 accattgaag tgggtaaaac aatgaatcct attgtgttga ctacaacgga taatggtact
    14341 gggactgtga caaatacagt tacaggatta ccaagcggat taagttatga tagtgcaacg
    14401 aattcaatca ttggaacacc aacaaaaatt ggtcaatcaa cagtgacagt agtatctact
    14461 gaccaagcaa ataacaaatc gacgacaact tttacaataa atgttgtgga tacgacagca
    14521 ccaacagtga caccaatagg agatcaatca tcagaagtgt attcaccaat atccccgatt
    14581 aaaattgcta cgcaagataa tagtgggaat gcagtaacaa atacagtcac aggattgccg
    14641 tctggattaa cattcgatag cacaaataat actattagtg gtacaccaac aaacattggt
    14701 acaagtacta taacaatcgt ttctacagat gcgagcggta acaaaacgac gacaactttt
    14761 aaatatgaag taacaagaaa tagcatgagt gattccgtat caacatcagg aagtacacaa
    14821 caatcccaaa gtgtgtcaac aagtaaagct gactcacaaa gtgcatcaac gagcacatca
    14881 ggatcgattg tggtatctac ttcagctagt acctcgaaat cgacaagtgt aagcctatct
    14941 gattctgtga gtgcatctaa gtcattaagc acatctgaaa gtaatagtgt atcaagctca
    15001 acaagcacaa gtttagtgaa ttcacaaagt gtatcatcaa gcatgtcagg ttcagttagt
    15061 aaatcaacat cattaagcga ttctatttcg aactctagca gtactgaaaa atccgaaagt
    15121 ctatcaacaa gcacatctga ttcattgcgc acatcaacat cgttaagtga ttcagtaagc
    15181 atgagtacat caggaagctt gtctaagtca caaagcttat caacaagtac atctgattcg
    15241 gctagtacat cacaatcggt aagtgatagt acatcaaatt caattagtac atcagaatca
    15301 ttgagcgagt cagctagcac ctcggactct atcagtattt caaatagtat agccaactct
    15361 caaagtgcgt caacaagcaa atcagattca caaagtacat caatatcatt aagtacaagt
    15421 gattcaaaat cgatgagtac atcagaatca ttgagcgatt caacgagcac aagtgattca
    15481 gtatctggat cactaagcat agcaacatca caaagtgtct caacaagctc atcagactca
    15541 atgagcacgt ctgaaatgat aagcgattct atgagtacaa gtggatcatt agctgcatca
    15601 gacagtaaat caatgtccgt aagtagttcg atgagcacgt ctcagtcagg tagtacatca
    15661 gaatcattaa gtgattcgat aagtacatcg gattctgaca gtaagtcatt atcattgagt
    15721 acaagtcaat caggttcaac aagcacatca acatcaacaa gtagttcagt acgtacgtcg
    15781 gaatcacaaa gtacgtctgg ttcaatgagt gcaagtcaat tcgattcaac aagcatatca
    15841 acgtcgttta gtgattcaac aagtgatagc aaatcagcat caactgcatc gagtgaatca
    15901 atatcacaaa gtgtctcaac aagtacatct ggctcaacaa gtggttcaac atcaacaagc
    15961 acatctgaat cgctaagtat gtctggttca gattcaacga gcgtatctga ctcaacatca
    16021 atgagtgaat ccgattcaac aagtgtttca atgtcacaag acaaatccga ttcaacatca
    16081 attagcgact cagaatcagt gtcaacaagc acatcaatgt cattgagcac atccgattcg
    16141 acaagcacat ccgaatcgct gagtacatct atgtctggtt cacaaagcat ttctgactca
    16201 acatcaacaa gtatgtctaa ctcgacttca atgagcaact caacatcaac aagtatgtcc
    16261 ggctcaacaa gtacatctga atctaactca atgcatccgt cagactcaat gagtatgcat
    16321 catacacaca gcacgagcac atctcgctca tcaagtgaag caacaacgag cacgagtgaa
    16381 tctcagtcta cattaagtgc aacatctgaa gtgactaaac ataatggcac gccagcacaa
    16441 agtgaaaaaa gattgccaga tacaggtgac tcaataaaac aaaatggatt actaggtggc
    16501 gttatgacat tattagttgg tttaggttta atgaagagaa agaaaaagaa agatgaaaat
    16561 gatcaagatg attctcaagc ataaattgaa tttatagttg gctaaaatta aaaagactgg
    16621 ggcgttgttc actaaaaaat ctatatcaga ttttaataat tagtgaaata accttagtct
    16681 ttttaatgtt tttgatataa aagaggtgca acatgttaaa acttttacaa caatacgaat
    16741 ataaaattat atataaacgt atactttaca catgttttat cttgtttatt tatatattag
    16801 gtacaaacat ttccatcgtt tcctataacg atatgcaagt aaaacatgag tcatttttta
    16861 aaatagctat ttctaatatg ggtggagacg ttaatacttt aaacattttc actttaggat
    16921 taggtccgtg gttaacatca atgattatct tgatgctgat ttcttatcgg aatatggata
    16981 aatacatgaa acaaacaagt cttgagaagc attataaaga gcgcatctta acattaattt
    17041 taagtgttat tcaaagttat tttgtgattc atgaatatgt ttcgaaagaa cgtgttcatc
    17101 aagacaatat ttacttaaca atcttgattt tggttactgg aacgatgtta ttagtttggt
    17161 tagctgacaa aaatagtcgt tacggcatcg caggaccaat gccgattgta atggttagca
    17221 ttattaaatc aatgatgcat caaaaaatgg aatatataga tgcaagtcat attgtgattg
    17281 cgttgttaat cactcttgtg ataatcacat tgtttatctt attattcatt gaattagtag
    17341 aagtgagaat accatatatc gatttaatga acgtttccgc aacaaatatg agatcttatt
    17401 tatcttggaa agttaaccct gcaggcagta ttactttaat gatgagtatt tcagcatttg
    17461 ttttcttgaa aagtggtatt cattttattt tatctatgtt taataaagac gtttcagatg
    17521 atatgccaat gatgacattt gatagtccaa ttggtatttc ggtgtattta gtgattcaaa
    17581 tgttattggg ttatttttta tcgagatttt taatcaatac taaacaaaaa tccaaagatt
    17641 tcttaaagag tggcaattat ttttcaggag ttaaacctgg taaggataca gaacgttatt
    17701 taaattatca agcacgacgc gtatgttggt ttggatcggc attagttaca gtcattattg
    17761 gtataccact ttatttcaca ttgtttgtac cgcatttatc tactgaaatt tatttctcag
    17821 tacaactgat tgtattagtt tacatcagta ttaatattgc agaaacaatt cgtacatatt
    17881 tatattttga taaatataag ccatttttaa accagtattg gtaaggaggt aattatgaaa
    17941 tactttattc cagcttggta cgatgaccaa cgatggtggc aagacacgac tgtgccgtat
    18001 tatcaactac aaaataagac ggaatttgac gatatgatta gtttgatggg gatgcacctt
    18061 gaaaatgact tagattatcg tctgattgtt ctcaatcatg caccaaattt aagaacattt
    18121 ttacatcgat atgacttata tgagacaaag tattggtctg tgtttgatga aattcaagga
    18181 ttcagtcacc atgcgccaca agcgattaat tatcatcact taaaatggcc ggatgatgtt
    18241 gagtttgtgt acacgccgta tttattaaaa tgtgtgacga gtgaacagac ctatacaaat
    18301 atttatttta gtcaagaagg gtattcaatt tggtttgaag aatttgaaag agatcagtta
    18361 caacggcgtt atatttttga tgacagaggt tatttatcag ctatacgtta ttttgatgat
    18421 cagggagagg cttcttacca agaatattta acgattaatg gagattgtgt acttcatgaa
    18481 gatttgaaaa atggcagagt cactgtttca aaaagatatc aacatcacta tcaacaaaca
    18541 gaatataaca atatggctca actaattgaa gaaaaatttc aagcaatgat tgcacatcaa
    18601 atacatgaag atgatcatgt gattgtggct tcagatgcta ggcacaatcg acaaatagcc
    18661 aatcatattc cggcgaaatc attaagttat tcatttttta aaaatagaaa tgaaactgtg
    18721 tcggatgagg aatatcaatc tatcgtaaag aatgcccatt taattgttga tagtgtgcaa
    18781 ctagaacgtg atttaattag tcatcaagag aagtatcagc gagagaatac aatgattcga
    18841 atcacgccat ttgaaacgag acaatcacct aatataagta gtcaattgat ggaaacattt
    18901 ataggtgtat ggatagacgg tatgagtgac gttgatttgc aacaaatgat gcaacgactt
    18961 gtggattata tcgcacaaga agataattac cgtttaattt tattaacgcg ccatcaaaat
    19021 gatataccga tgtggcttcg tgaatgtatt acgtcggtaa atgaggaata ccaagctaaa
    19081 cagaatgccg atgttaatgt ttcagcatta atgacacctg aagatcaaga tgacatcatt
    19141 gctgttaaga cgatacatgc tgaacatgat gttgtagaag cattgcggac gttgcgactt
    19201 gtgatagata tgtcaaaaga acctgatttg tatttacaaa ttagtgcaat tagcgctggg
    19261 attccacaaa ttaatggtca acaaacagat taagtctctg attatgacaa tggccgtatt
    19321 ataaatacag ttgatgaatt agatgatgcg ttaaattatt atttatttta tttgaaaaat
    19381 tggaattatg cgtacgccta ttctttaaaa ttaatagatg catatgcttc taagaatatt
    19441 attaatcagc tcgatgagtt aatagaaggt gaaaatgatg ccacgtaaat ttagagtttt
    19501 gcaaattgga ggagacgatt tagaacctat tttccaacac aaaaaaggtg tgagttggga
    19561 ttacttcgat attggattgt ttgaatttga tagtggttat gtagaggcta ttgaagcgat
    19621 tgttgaagca gaaggacgct ttgattttat ctatattcaa gcaccatact cggagacatt
    19681 aacgaattta ttacaaatga taagcgaacc atacaatacg tatgttgatg aatcattttg
    19741 gtctgttgaa tatgaacaag acgaaaatgt ccaaaaatac gttgttcaac cattacatta
    19801 ccggaacatt gaagaacgta ataataaatt agaggcagtt agcttctcag gacagtatgg
    19861 agataaggtt tctcctaagt tagcacttgt tcatccgaat tttaaaggag atgtcgttta
    19921 ccaaggtaat tcagagctca cgttgagtgg agaatttgga aaagaattta aacctatcgc
    19981 atcttggcaa aataatctcg tttacgataa agataaagtc attcaaatat ggccagaatt
    20041 tgatattgat ggtgcggtcg agttgcaata tacatttaga ttgattcaaa ctggcgctga
    20101 tggtgcatta attgaacaaa tcatattgac tgatgatatg ttagacagtc ctttagagat
    20161 acctacgaaa ccatttgatg cttatataag tgtaactgtt aaggcgcgag ggaacgggac
    20221 ggtacattta ggacctatac acaaacgatg gtctagatta gacatgggtc aatttttact
    20281 cggtggtagt cgttttgtag atagtcaacg acaggaattt atttattatt tccaccctgg
    20341 tgatatgaaa ccaccactaa acgtatattt tagtggttat cgaacagcgg aaggtttcga
    20401 aggatattat atgatgaaac gtatgaatgc acccttttta ctaatcggcg atcctcgtgt
    20461 tgaaggtggt agcttttata tcggttcatc tgaatatgaa caaggtatta tcaatgttat
    20521 tgaagagaca ttagaaaagc tgaattttaa atcacatgaa ttaatattat ctggtttatc
    20581 tatggggtca tttggtgcct tgtattatgg tgctcagtta aatcctcagg caattattgt
    20641 tggtaaacca cttgttaata ttggaacgat tgctgaacat atgcgattac ttagaccaga
    20701 agagttcggt acagcattag acgtcctggt gtctaatgaa ggtgatacat ctcaagcgtc
    20761 gatacaggca ttaaatcaaa aattttggca aacatttcaa aagaaatcat tgtcacaaac
    20821 tgtttttgcc atagcatata tgcaacatga tgattatgat ccacatgcat ttcaagagct
    20881 actacctgta ttgacagcac accaagcaag agtgatgaat cgcagtattc ctgggcgaca
    20941 taatgatgat tcacctacca ttgcaagttg gtttgttaat ttttacaaca ttattttgga
    21001 agacaagttt gggagagttc aacatgctga aaaacaaaac atttaaagtt acttggaatt
    21061 atatttcaca aactacattt atgtatggct caaaagtatc attttcaaat ggtgaagtaa
    21121 cttttataaa tcctttgatg ccctctggat taccgattca tgagtggttg atgttaaaac
    21181 aattttcaaa atataaaagt gcaccaagtt taccaatttt gcgtagagga cagcattata
    21241 aattgcattt tgattttgat gcaacgcctg ctggttcagt gtattttatc atcatttttt
    21301 ataataaaaa tggcacaaaa ctaagtactg agattgtaaa gtcaaatagt attacgattc
    21361 aatatcctga tgaagcctat gcttataaaa taaaaatgat gaatgcagca tcgacgagtc
    21421 ttatatttag atgtttaaca attacagaga tgacgcatca agataattta gaatataaaa
    21481 gcatgcgggt gacgaagata ggcgatgacc aatatggaaa tgataggatc aatgtcatta
    21541 ttgcagaacc atctgacgcc tatccaacta tttcgaatga ttttttgaaa tcgtttggtc
    21601 atgtttggct cgttgaacgt tggatggacg acaatatcga gggaaatatt aagcaactaa
    21661 agaatgattt gcaaagtcaa gataccctaa aggcaattaa tttgattagt tatgggtcaa
    21721 agtcaaatgt ttttgcaact tatgtcgctc agcatttgga ttgcaaagtg tatcgtacaa
    21781 gccatgaaga tgatgattta aaagaatggt ttacagaaca tgtcccagga aatcatgaac
    21841 taaaagatac aaatgtcgag gtttatttta aagaagagca agacaaccat ctaaattata
    21901 tgtcacgttt aatgaatcca gtacgatttt tagattactt agatgtatcg aaactgaatg
    21961 gtggggaagt aaatgaaaca taaactagat gttacgataa acgaattaag actaaagtcg
    22021 attagaaaaa tagtgaaacg catcaataca tggagcgatg aagttaaatc atattctgat
    22081 gatgtgttaa aacaaaagac actagaattt aaagaacgta tagcatcagg agttgataca
    22141 ctagacacat tgttacctga agcttatgca gtggcacgtg aagcgagctg gagagtatta
    22201 ggtatgtatc ctaaagaagt tcagttaatc ggagcgattg tattgcatga aggtaacatt
    22261 gcggagatgc aaacaggcga aggtaaaaca ttaacagcaa cgatgccttt atatttaaat
    22321 gcactttcgg gaaaaggaac atatttaatt acaacgaatg attatttagc gaagcgtgat
    22381 tttgaagaga tgcaaccatt atatgaatgg ttaggtttaa ctgcttcatt aggttttgtt
    22441 gatattgtgg attatgagta tcaaaaaggg gaaaaacgta atatatatga acatgatatt
    22501 atatatacaa ctaatggacg tctcggtttt gattatttaa ttgataattt ggcagatagt
    22561 gcggaaggga aatttttacc acaattaaat tacggtatta ttgatgaagt ggattcaatc
    22621 attttagatg ctgcgcaaac accattagtt atttcgggcg caccaagatt acaatcaaat
    22681 ctatttcata ttgtgaaaga gtttgtagat acattgattg aagacgtgca ttttaaaatg
    22741 aagaagacca aaaaagaaat atggctgtta aatcaaggta ttgaagcggc acaatcatac
    22801 tttaatgttg aagattta
//
LOCUS       DAMZPP010000027        22645 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMZPP010000027 DAMZPP010000000
VERSION     DAMZPP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22645
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.027"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..188
                     /locus_tag="POH73_000817"
     CDS             <1..188
                     /locus_tag="POH73_000817"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HDI6886790.1"
                     /translation="IRHLIKAEETFGIRLTTIHNLHFLLKLMEDIRQAIREDRLLDFK
                     EEFFEQYGLNVENPKNF"
     gene            207..467
                     /gene="yajC"
                     /locus_tag="POH73_000818"
     CDS             207..467
                     /gene="yajC"
                     /locus_tag="POH73_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDI6886791.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            742..3021
                     /gene="secDF"
                     /locus_tag="POH73_000819"
     CDS             742..3021
                     /gene="secDF"
                     /locus_tag="POH73_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HDI6886792.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQYWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKGSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
     gene            3224..5497
                     /gene="recJ"
                     /locus_tag="POH73_000820"
     CDS             3224..5497
                     /gene="recJ"
                     /locus_tag="POH73_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDI6886793.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTSTTILVTT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEQFNRERKDIVATITEEAMAMA
                     EMKVKNGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     PVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQLIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDVFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            5519..6037
                     /locus_tag="POH73_000821"
     CDS             5519..6037
                     /locus_tag="POH73_000821"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDI6886794.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
     gene            6465..8654
                     /locus_tag="POH73_000822"
     CDS             6465..8654
                     /locus_tag="POH73_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDI6886795.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            8666..9118
                     /locus_tag="POH73_000823"
     CDS             8666..9118
                     /locus_tag="POH73_000823"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDI6886796.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQDSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVNINNDGPVTIIYESQDGKIQ"
     gene            9115..9990
                     /locus_tag="POH73_000824"
     CDS             9115..9990
                     /locus_tag="POH73_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDI6886797.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            10451..11713
                     /locus_tag="POH73_000825"
     CDS             10451..11713
                     /locus_tag="POH73_000825"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDI6886798.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            11729..13495
                     /gene="aspS"
                     /locus_tag="POH73_000826"
     CDS             11729..13495
                     /gene="aspS"
                     /locus_tag="POH73_000826"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDI6886799.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWINNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFEAENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            13573..13763
                     /gene="ssrS"
                     /locus_tag="POH73_000827"
     ncRNA           13573..13763
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="POH73_000827"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            13828..13956
                     /locus_tag="POH73_000828"
     CDS             13828..13956
                     /locus_tag="POH73_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886800.1"
                     /translation="MGLIAFNNCFLVEFWKNNQSNNAKHKILSYYVLMYKSVKEVK"
     gene            13956..14729
                     /locus_tag="POH73_000829"
     CDS             13956..14729
                     /locus_tag="POH73_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDI6886801.1"
                     /translation="MKHQFSRNELAIGHQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(14890..16164)
                     /locus_tag="POH73_000830"
     CDS             complement(14890..16164)
                     /locus_tag="POH73_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDI6886802.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDEENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSERQLKEIYNNLLKQRP"
     gene            16249..16671
                     /locus_tag="POH73_000831"
     CDS             16249..16671
                     /locus_tag="POH73_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDI6886803.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            16771..16953
                     /locus_tag="POH73_000832"
     CDS             16771..16953
                     /locus_tag="POH73_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDI6886804.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKATGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            16993..17139
                     /locus_tag="POH73_000833"
     CDS             16993..17139
                     /locus_tag="POH73_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886805.1"
                     /translation="MSFIDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
     gene            complement(17375..18388)
                     /locus_tag="POH73_000834"
     CDS             complement(17375..18388)
                     /locus_tag="POH73_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDI6886806.1"
                     /translation="MAIKYSALNLVPIREGEDERTAINDMVKLAQHLDELSYERYWIA
                     EHHNTPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQLNEK
                     NTL"
     gene            18698..19840
                     /locus_tag="POH73_000835"
     CDS             18698..19840
                     /locus_tag="POH73_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDI6886807.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            19841..20959
                     /gene="mnmA"
                     /locus_tag="POH73_000836"
     CDS             19841..20959
                     /gene="mnmA"
                     /locus_tag="POH73_000836"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDI6886808.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            21641..22309
                     /locus_tag="POH73_000837"
     CDS             21641..22309
                     /locus_tag="POH73_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDI6886809.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEEDPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFYNLANVYYNQQRYQESIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNENIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            22311..>22645
                     /locus_tag="POH73_000838"
     CDS             22311..>22645
                     /locus_tag="POH73_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDI6886810.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQ"
BASE COUNT         8147 a         3180 c         4302 g         7016 t
ORIGIN      
        1 atatacgtca tttaatcaag gcagaggaaa cttttggtat tcgtcttact actattcata
       61 atttacattt tctgctaaaa ttaatggaag atataagaca agccattcga gaagatcgtc
      121 ttttagattt caaagaagaa ttcttcgagc aatatggatt aaatgttgag aacccaaaaa
      181 acttttaagc aagaggagcg tataaaatgc aattttcatt acttatatat atagtcgtaa
      241 tttttgcggt tatgtatttc ttgatgatca gaccacaaca aaaacgtgcg aaacagcatc
      301 gtgagttgat taataacatt caatctggtc aaagaattac aactattggt ggtattaaag
      361 gtactgttaa agcagtagat gaaacaactg ttgttattac agttaatggt catggtactg
      421 aattaacttt cgaaaaacct gctattaaac aagttgaccc ttcataacaa gaagtggtat
      481 taaaaaggat gaaacgaata gcgtttcatc ctttttaagt tataagtagt atgtagaata
      541 gcttattttt attccaaaaa aagaaattgt gatacataat aattgttaaa tttaaattaa
      601 atatatctat gactattttt actccctgct ctagtatgtt aatatgtata aggctaataa
      661 attttgtaag aaagatttaa cttgcgttat aaaatagtat tcaagtattc taaatacata
      721 cgtaaataat gaggtgttca tgtgaagaaa agtagtagaa taattgcgtt cttgctttta
      781 gtcgtattgt tgtttgccgg aatggctgca acttataaaa gtgttataaa gaatgtcaat
      841 ttaggacttg atttgcaagg tggatttgag gtgctttatc aagtcgaccc tttaaataaa
      901 ggcgacaaaa tagataaaaa agccttacaa tcaacagcgc aaacattaga aaatcgtgtt
      961 aacgttttag gtgtttccga acctaaaatt caagttgaag aacctaatag aattagagtt
     1021 caacttgctg gtgtaacaga ccagaatgaa gcacgtaaaa ttttatcttc tcaagctaat
     1081 ttaacaattc gcgatgcaga agataaagta aaattaagcg gttctgatat taaacaggga
     1141 tctgccaaac aagaatttaa acaggaaaca aaccagccaa cagttacttt caaagtaaaa
     1201 gacaaaaaca aatttaaaaa agtaacggaa gaaatctcta agaaacgtga taatgtcatg
     1261 gttgtttggt tagatttcaa aaaaggtgac agctataaaa aagaagcaca aaagaaaaac
     1321 cctaaattta tttctgcagc aagtgttgat caaccaatca attctgatag cgtagaaatc
     1381 tctggtggat ttaaaggaca agaaggcgtt aaaaaagcaa aacaaatcgc cgaattgtta
     1441 aatgcaggtt ctttaccagt tgacttgaaa gaaatttatt caaactcagt aggtgcccaa
     1501 tttggtcaag atgcattaga taaaactgtg tttgcatcgt ttattggtgt agcattaatt
     1561 tatttattca tgcttggatt ctaccgatta cctggtttag tagcgattat tgcattgaca
     1621 acttatatct atctaacgtt agtagcattt aatttcattt ccggggttct aactttacca
     1681 ggattagcgg cgttggtatt aggtgtaggt atggctgtag atgccaatat tatcatgtat
     1741 gagcgtatta aggatgaact tcgaataggt agaacgataa agcaagcctt ttctaaagca
     1801 aacaaaagtt cgttcctaac aatttttgat tctaacttaa caacagttat cgccgcagca
     1861 gtattattct tcttcggtga aagttcagtt aaaggtttcg cgacaatgtt attattaggt
     1921 attctaatga tctttgttac agccgtgttc ttatcaagat tcttattatc attacttgtt
     1981 tcatcaaata tattcaaaaa tcaatattgg ttatttggtg ttaaaaagaa taaacgacat
     2041 gatattaatg aaggtgtaga tgttcatgac cttaaaactt cattcgagaa atggaatttt
     2101 gttaaattag ctaagccatt aattggagta agtattttaa ttgtagttgt cggtttagtt
     2161 attctttata tcttcaagtt aaacctagga attgatttct caagtggtac acgtgtagat
     2221 ttccaatcta aacaagcaat tactcaacaa aaggttgagc aagttgtaaa aggtagtgga
     2281 cttaaagcgg atcaaattca aatcaatggt aaagataata aagtagcaac tgttcaattt
     2341 aaagacgatt taacgcgtgc tcaagataat aaattgagtg ataatattaa atcgaaattc
     2401 ggtgacacac ctcaaattaa cactgtatca ccaattattg gacaggaatt agcaaaaaat
     2461 gcaatgcttg cattaatcta tgcatcgata ggtatcatca tctatgtatc attacgattt
     2521 gaatggcgca tgggtctttc atctgtattg gcattattac atgatgtatt cattatagta
     2581 gcgattttca gtttatttag aattgaagta gatttaacat ttatcgccgc tgtattaaca
     2641 attgtcggtt attcaattaa tgatacaatc gtaacgtttg accgtgtacg tgaaaactta
     2701 caaaaggtta aagtgattac tacaacagaa caaattgatg atatcgttaa tagatcaatt
     2761 agacagacaa tgacacgttc aattaataca gtattaacag ttattgtagt agtagttgct
     2821 atactattct tcggtgctcc tacgatattc aactttactt tagcattatt tatcggattg
     2881 atttctggtg tattctcttc aatcttcatt gccgttccgc tttggggaat aatgaaaaaa
     2941 cgtcagttga aaaaatcgcc gaaacacaaa ttagttgtat ataaagaaaa gaaatcgaac
     3001 gatgaaaaga ttttagttta aaatgaatta agcggtatgt gaaacattaa agagttcaca
     3061 taccgctttt tactttgttt aaatttaaca aaatatatat aaataagttc ccgcattcat
     3121 ttttaaaaaa tggtataatt aaagtcttga ttatttagat aagtgctttt tatttaaagt
     3181 aattttagat ataatgtctt ttggaaatga ggcgtaaata tatatgatta aaccgaagta
     3241 taagtggaaa ttaactaaac cagctgaata cataagtgat gaattaacat caaaattaaa
     3301 gttaacgcca atcgtaaaaa aaatattaga aagtaaatcg attattgatg aacaagcaat
     3361 tgaatccatc atttcagata cagatatcaa tcatgacgca ttgcaattga gcgatatgac
     3421 taagactatt gaaagaatta aaagagcgat agcgaatgat gagaaaatcc tagtttatgg
     3481 tgattacgat gccgacggtg ttacctcaac tacaatttta gtgacaacat tacaactttt
     3541 aggtgcacaa gtaggatggc atattcctaa ccgctttacc gaaggatatg gaccaaacga
     3601 gctagcattt agaaatgctc atgatgaagg cataacatta atcataactg ttgataatgg
     3661 catacaaggt cataatgaaa ttaaaatggt tcaagattta ggagttgatg tcattgtaac
     3721 tgatcatcat gaaattggta gtacattacc agaagcatat gcaatcgtac atccaatgca
     3781 tccatcgttt aattatccgt tccaacaatt gtgtggtgca ggtgttgcgt acaaattagc
     3841 acaagcattg attgaaaatg tacctgatta ttttaaagca ttagttgcga taggtaccat
     3901 tgcggattta gtatcattaa ctgatgaaaa tcgatcatta gtgaaacaag gattaaaagt
     3961 cttaaatgat caatgtccga cctctgttaa agcattgcta aaagaagcgg gatacaatga
     4021 caatattgat gaagaaacaa ttggttttat tattggacct agacttaatg ctgtaggtcg
     4081 tttagatgac gcatcattag cttgcgaatt gttgatgact gacgacgaag aagaagctgc
     4141 atttttagca gaacaagttg aacaatttaa ccgcgaaaga aaagatattg tagcaactat
     4201 cacagaagaa gcaatggcaa tggcagaaat gaaagttaaa aatggagatt tatttttact
     4261 tttagcaaaa gaaaattggc atgaaggtgt cttaggtatt gttgcttcga aaattgtcga
     4321 aacatttgct ttgccaacat taattttaaa tattgatagg gagcaaaatc atgcaaaagg
     4381 ttccgccaga tcaattgatc aagtgtcgat gtttgaaata ctatcagcac accaagagtt
     4441 aatcgctaaa tttggcggcc atcatatggc tgcaggtatg acgatggata ttgaaaatat
     4501 agaatcgtta gctgaaggtt taaataaatg gatgaaagaa ttatctaaaa caacttcgtt
     4561 agatcccgta aagccagtag atgtattatt aacagaaaat gatataacga ttaaaaatat
     4621 tcgtgatatg aatcgtttaa gacccttcgg aacagatttt tctagaccta tttttgaaat
     4681 ggatgattta tcagtttctt cagttaaggc gataggtcaa caaaaaaatc atctgaaatt
     4741 gacacttggt gaaagtaata tcgccgcgct attttggcaa aatggacact tagaacctga
     4801 attacaagat gaacagccaa ttaatatatt aggatctgtt caaatcaacg aatggaatgg
     4861 taatcaatca ccgcaattaa ttattcaaga tattgcgatg aacgaacagc aaatattaga
     4921 ttatagaagt aagcgaaaaa gtttaccttt tacagaaaat gatgaaaata ttgtcgtgct
     4981 tattcatcct aaaagtgata aagtaaatgc gaatgaatat tattatggtg aagaaattaa
     5041 acaacaaact gataaagtag tattaagaga tttacctacg tcaatggaag atttgtctaa
     5101 ttccctgcaa caactgcaat tttctcaact ttatatagtt ttgcaacata atcattcgat
     5161 ttacttcgat ggtataccta atatggatgt ttttaaaaag tgttataaag cattaataac
     5221 taaacaagaa acaaatatcc agaaagaggg tatgttattg tgtcaacatt taagtgtgaa
     5281 accagataca cttaaattca tgttgaaagt tttcttagac ttaaaatttg taacacaaga
     5341 agatggttta attcgaatca atcaacaacc tgataaaaga tcgattgatt ccagcaaagt
     5401 atatcaatta agacaacaac gtatggatgt tgaaaagcaa ttattatatc aagatttttc
     5461 agaaataaaa aattggataa agtcacaatt gtcgtgagca atttaggagg aaatattaat
     5521 ggatttaaag caatacgtat cagaagttca agattggccg aaaccaggtg ttagtttcaa
     5581 ggatattact acaattatgg ataatggtga agcatatggc tatgcaacag ataaaattgt
     5641 agaatacgca aaagacagag atgttgatat cgttgtagga cctgaagcgc gtggctttat
     5701 cattggctgt cctgtagctt attcaatggg gattggcttt gcacctgtta gaaaagaagg
     5761 gaaattacct cgtgaagtca ttcgttatga gtatgaccta gaatatggta caaatgtttt
     5821 aacaatgcac aaagatgcaa ttaaaccagg tcaacgtgtg ttaattacag atgatttatt
     5881 agctactggt ggtacgattg aagcagcaat aaaattagtc gaaaaattag gtggtatcgt
     5941 agtaggtatt gcatttataa ttgaattgaa atatttaaat ggtattgaaa aaattaaaga
     6001 ttacgatgtt atgagtttaa tctcatacga cgaataaaaa ataatataat tttatcaaat
     6061 aaaatccttc atcaaatgaa taagaacgaa tgacttaatt aaaaaagttg tttaagtttt
     6121 cttaacatga gatgttagga ttttttattt actgaaaatg ttagatgatt gagcattata
     6181 ccttaataac aacgtttatt tatttcataa attgtagtat catagaacta atatttaaaa
     6241 aatgaaacag tagatttagg tcgaattttt gtaaaagttt taaaagtagg aatagtatac
     6301 aaattaaact cgctcaagta aaattaatat tacgattaat gacgacagga taaatattta
     6361 tcgtcgacgg acgtatgatt ggtgtgggac aaatactatt caacaagagc acctaaatca
     6421 ttgtttaagg cgaagtaata aatatgaatg gggtgtatca tataatgaac aacgaatatc
     6481 catatagtgc agacgaagtt cttcacaaag caaaatcata tttgtcagca gatgaatatg
     6541 agtatgtttt aaaaagctat catattgctt atgaagcaca taaaggtcag ttccgaaaaa
     6601 acggattacc atacattatg catcctatac aagttgcagg tattttaaca gaaatgcgat
     6661 tagacggacc gacgattgtc gcaggttttt tgcatgatgt aattgaagat acaccgtata
     6721 catttgaaga tgtaaaagaa atgttcaatg aagaagttgc tcgaattgtt gatggtgtga
     6781 cgaagcttaa aaaagtaaaa taccgatcaa aagaagaaca acaagctgaa aatcatcgca
     6841 agttatttat tgcgattgcc aaagatgtac gcgtaatttt ggtgaaatta gcagacagat
     6901 tacataatat gcgtaccttg aaagccatgc cgcgcgaaaa acaaattaga atttctcgag
     6961 aaacattaga aatttatgca cctttagcac atcgtcttgg tattaataca atcaaatggg
     7021 aactagaaga tacggctctt cgttatattg ataatgtaca atattttaga atagtcaatt
     7081 taatgaagaa gaaacgtagt gaacgggaag cgtatatcga aacggctatt gatagaatac
     7141 gtaccgaaat ggaccgaatg aatatcgaag gcgatataaa tggtagacct aaacatattt
     7201 acagtattta tcggaaaatg atgaagcaga aaaagcaatt tgatcaaatt tttgatttgt
     7261 tggcgatacg tgttattgtc aattctatta atgattgtta tgcgatactt gggttggtgc
     7321 atacgttatg gaaaccgatg ccaggacgtt ttaaagatta tattgcaatg cctaaacaaa
     7381 atttgtatca gtcattgcat actacagtag taggtccaaa tggagacccg ctcgaaatcc
     7441 aaatacgaac gtttgatatg cacgaaattg ctgaacatgg tgttgcagca cactgggctt
     7501 acaaagaagg taaaaaagta agtgaaaaag atcaaactta tcaaaataag ttaaattggt
     7561 taaaagagtt agctgaagcg gatcatactt cgtctgacgc tcaagaattt atggaaacct
     7621 taaaatatga cttacagagt gacaaagtat atgcatttac cccagcgagt gatgttattg
     7681 agttgccata tggtgctgtg ccgattgatt ttgcttatgc gattcacagt gaagtaggta
     7741 ataagatgat tggtgccaag gtgaatggca aaattgtacc aattgactat attttacaaa
     7801 caggcgatat tgttgaaata cgtactagta aacattcata tggaccaagt cgtgattggt
     7861 tgaaaattgt taaatcgtct agtgccaaag gtaaaattaa aagtttcttc aaaaaacaag
     7921 atcgttcatc taacattgaa aaaggacgaa tgatggttga agctgaaata aaagagcaag
     7981 gatttagagt cgaagatatt ttgacagaga aaaatattca ggttgttaat gaaaaatata
     8041 actttgcaaa tgaagatgat ttattcgcag ctgtaggatt tggcggcgtg acatccttac
     8101 agattgttaa taaattaact gaaagacaac gtattttaga taagcaacgt gctttaaatg
     8161 aagcacaaga agttacgaaa tcattgccta ttaaagacaa catcattact gatagtggtg
     8221 tctatgtaga aggtttagaa aatgtactta tcaagttgtc aaaatgttgt aatcctatac
     8281 caggtgatga tattgtaggt tatatcacca aaggtcatgg tattaaagta catcgcactg
     8341 attgcccaaa tattaagaac gaaactgaac gactaattaa tgttgaatgg gtaaaatcaa
     8401 aagacgcaac tcaaaaatat caggttgatt tagaggtaac tgcgtatgac cgaaatggct
     8461 tgttaaatga ggtactacaa gctgttagct cgacagccgg caatttaatt aaagtttcag
     8521 gtcgttcaga tattgataaa aatgcaataa taaatattag tgtcatggtg aaaaacgtga
     8581 atgatgttta tcgtgtggta gaaaagatca aacaacttgg tgatgtttat acagtaacaa
     8641 gagtttggaa ctagaggtgc aaaatatgaa agtagttgta caaagagtta aagaagcatc
     8701 ggtgacgaat gatacattaa ataatcaaat caaaaaagga tattgtttat tagtcggtat
     8761 cggtcaggac tctacagagc aagatgcaga tgtaattgca aagaaaattg ctaatgcaag
     8821 attatttgaa gatgacaata ataaattaaa ctttaatatc caacaaatga atggtgaaat
     8881 attatcagtt tcacaattta ctctctatgc agatgtaaaa aaaggtaacc gtccaggttt
     8941 ctcaaattct aaaaatcctg atcaagcagt aaaaatatat gagtatttta atgatgcgct
     9001 acgagcgtat ggtctaactg tgaaaacagg tgaatttgga acacacatga atgttaatat
     9061 aaataatgat ggtccagtca ctattattta tgaaagtcag gacggcaaaa ttcaatgaaa
     9121 aaaatagagg catggttatc taaaaagggt cttaaaaata aacgtactct aatagtagtg
     9181 attgcctttg tcttatttat catcttttta tttttattgc tgaatagcaa tagtgaagat
     9241 agtgggaaca ttacgataac tgaaaatgct gaattacgta caggtccaaa cgctgcgtat
     9301 ccagtcattt acaaagttga aaaaggtgac cattttaaaa agattggtaa agtaggtaaa
     9361 tggattgaag ttgaagatac atccagtaat gaaaaaggtt ggatagctgg atggcacaca
     9421 aatttagata ttgtcgcgga taatacgaag gagaaaaatc ctttgcaagg taaaacaata
     9481 gtgcttgatc ctggtcatgg aggtagtgac cagggtgctt caagcaatac taaatataaa
     9541 agtttagaaa aagattatac gttgaaaaca gcaaaagaat tgcagcgtac tttagaaaaa
     9601 gaaggcgcaa ctgttaagat gacaagaaca gacgatacat atgtttcact agaaaatcgt
     9661 gatatcaaag gcgatgccta tttgagtata cataatgatg ccttagaatc atctaatgca
     9721 aatggaatga cagtttattg gtatcatgat aatcaaagag ctttagcaga tacgttagac
     9781 gctacgattc agaagaaagg tctactttct aatcgcggtt caagacaaga aaattatcaa
     9841 gtgttaagac aaacaaaagt tcctgctgtt ttattagaat taggttatat tagtaaccca
     9901 actgatgaaa cgatgattaa agatcaatta catagacaaa ttttagaaca agcaattgtt
     9961 gatggcctta aaatttattt ttctgcgtag ggcttgcaaa aatatgtgaa agtagttatc
    10021 attgatattg aattttataa ctaaaaccgt tagtattctt gaaatggtaa atgaaatagg
    10081 tagcaatcta actaagattg tgtaggaata taatccatag actgaaagat tatgctgagt
    10141 agtttatata cattgaacac aagaagaggt gctttatgaa aagtaaagcc gttaaacgta
    10201 cgttaaacgt tttgagtggg tttattaaat gaacgcttat aaaaagtaat gatgattaca
    10261 attaggcatg ttttttaaac caatgagggt ggcaacacgg atattcgtcc cttgtgcagt
    10321 ttcaggctgt gcaagggctt tttcttttgc ttttaaaatt atgatttatc gtgcaaagtt
    10381 aagtggtcgt atatagtttt agttttaaaa aggtaattaa ataaaatagt ttgccgaggg
    10441 agatgtcaaa atgattaaaa tacctagagg gacgcaggat attttacctg aagattcaaa
    10501 gaaatggcgt tacattgaaa atcaattaga tgaattaatg acattttaca attataaaga
    10561 aataagaaca ccaatttttg aaagtacaga tctttttgca agaggtgttg gtgattcaac
    10621 cgatgtcgta caaaaagaaa tgtatacatt taaagataaa ggcgatagaa gtattacatt
    10681 aagacctgag ggaacagctg cagttgtgcg ttcatatatt gaacataaaa tgcaaggtaa
    10741 tccaaaccaa ccaattaaac tttattacaa tggaccgatg tttagatatg aacgtaagca
    10801 aaaagggcgc tatcgtcaat ttaatcaatt tggtgtagaa gctattggtg ctgaaaatcc
    10861 tagtgtagat gcagaagtat tagctatggt tatgcatatt tatcaatcat ttggattaaa
    10921 acatttaaag cttgttatta atagtgtagg ggatatggcg tctcgaaaag aatataacga
    10981 agcgttagtg aaacactttg aaccagtaat tcatgaattt tgttcagatt gtcaatcacg
    11041 tttgcataca aatccgatgc gaattttgga ttgtaaagtt gaccgtgata aagaagcgat
    11101 taagactgca cctagaatca ctgatttctt aaatgaggaa tctaaggcat attatgaaca
    11161 agtaaaagct tatttagatg atttaggtat tccatatatt gaagatccta acttagttcg
    11221 tggattggat tattatacac atacagcatt tgaattaatg atggataacc ctaactatga
    11281 tggtgccatt acaactcttt gtggtggtgg ccgttataat ggtttattag aattgctaga
    11341 tggtccaagt gaaacaggta ttggttttgc gctaagtata gagcgattat tgcttgcact
    11401 tgaagaagaa ggtatcgaat tagatattga agaaaactta gatttattca ttgttacaat
    11461 gggtgatcaa gcagatcgat atgctgtgaa gttattaaat catttgagac ataatggtat
    11521 taaagcagat aaagactact tacagcgtaa aattaaagga caaatgaaac aagcagaccg
    11581 tttaggtgcc aagtttacaa tcgttattgg tgatcaagaa ttagaaaata ataaaatcga
    11641 tgttaaaaat atgacaactg gtgaatctga aacaattgaa ttagacgcat tagtcgaata
    11701 ttttaagaag tagagagggc gttaaaatat gagtaagaga acaacttatt gtggattagt
    11761 tactgaagca tttttaggac aagaaattac attaaaagga tggattaaca atcgtcgtga
    11821 cctaggtgga ttgattttcg ttgatttaag agatagagaa ggaattgtac aagttgtgtt
    11881 taatcctgca ttttcagaag aggcattgaa aattgctgaa acagtacgtt ctgaatatgt
    11941 tgtagaagtt caaggtacag ttacgaagcg tgaccctgaa actgttaatc ctaaaattaa
    12001 aactggccaa gttgaagtac aagttacaaa tattaaagtg attaataaat ctgagacacc
    12061 accattttct ataaatgaag aaaatgttaa cgttgatgaa aatattcgat taaaataccg
    12121 ttatttagat ttacgtcgtc aagagttagc gcaaacattt aaaatgagac atcaaattac
    12181 acgttctatt cgtcaatatt tggatgatga agggttcttt gacatcgaaa caccagtact
    12241 aacgaagtca acacctgagg gtgcacgtga ctatttagta ccatctcgtg tccatgatgg
    12301 tgaattttat gcattaccac aatcgccaca attatttaag caattattga tgattagcgg
    12361 atttgacaaa tactaccaaa ttgtaaaatg cttccgtgac gaagatttac gtgcagatcg
    12421 tcaacctgaa ttcacacaag tcgatattga aatgagtttt gtagatcaag aagatgtgat
    12481 gcaaatgggt gaagaaatgc ttaaaaaagt tgttaaagaa gttaaaggcg ttgaaattaa
    12541 tggcgctttc ccacgcatga catataaaga agcgatgcgt cgctatggtt ctgataaacc
    12601 agatacgcgt tttgaaatgg aattaattga cgtttctcaa ttaggacgtg atatggactt
    12661 taaagtattt aaagatactg ttgaaaatga tggtgaaatt aaagcaattg tcgctaaagg
    12721 tgcagctgaa caatatacac gtaaagatat ggatgcttta acagaatttg taaacatcta
    12781 tggtgctaaa ggattagcat gggttaaagt tgtggaagat ggtttgacag gtccaattgg
    12841 acgtttcttt gaggcagaaa atgttgaaac attacttaca ttaactggtg ctgaagctgg
    12901 tgacttagta atgtttgttg cagacaaacc aaatgtcgtt gcacaaagtt taggtgcatt
    12961 acgtgtcaaa ttagctaaag aattaggttt aatcgatgaa acaaaattaa acttcttatg
    13021 ggtgacagat tggccattat tagaatatga tgaagatgcg aaacgttacg ttgcagcaca
    13081 tcatccattt acatctccaa aagaagccga tattgctaag cttggcactg cgccagaaga
    13141 agctgaggca aatgcttatg acatagtatt aaatggttat gaattaggtg gcggttcaat
    13201 cagaattcat gacggtgagt tacaagaaaa aatgttcgaa gttcttggat ttactaaaga
    13261 acaagcacag gaacaattcg gcttcttact agatgcattt aaatatggtg caccacctca
    13321 tggcggtatt gcattaggat tagaccgatt agtcatgtta ttgactaata gaacaaactt
    13381 gagagataca attgcattcc ctaaaacagc atctgctaca tgtttattaa cgaatgcgcc
    13441 tggtgaagtt tctgataaac aattagaaga actttctttg cgaattcgtc actaaatttt
    13501 aaaaaatcaa taaactttgg tttgttataa tttcattaat tttaattatg tggtaatata
    13561 acatcataag atagtcatct gagttgttcg taagtttgct ttttaatttg ggcctaacac
    13621 tctttgatca agggagccca atgggttttc ttgcagcgta cacgccttat atgaaggacg
    13681 tacaaaacaa gaaacagggc acccacctgt atataacagg ccgaatgatc aagctattat
    13741 aactacggca atacggactc tatcgatacg caagactttg tcttgcgtat ttttatgctt
    13801 ttctttaagt ttttaataaa gaattagttg ggtttaatag cgtttaataa ttgtttcctc
    13861 gttgaatttt ggaaaaataa tcaaagcaat aatgctaaac ataaaatttt aagttactat
    13921 gtacttatgt ataaatcagt gaaagaggta aaataatgaa acatcaattt tcaagaaatg
    13981 aactagcgat tggacatcaa ggtttagatt tattgaagca acaaactgtt gtcatacttg
    14041 gcgttggagg tgttggttca tttgctgcgg aagcattagc aagaacgaat attggtcata
    14101 ttatattaat agataaagat gatgttgata ttacaaatgt aaacagacaa ttacacgctt
    14161 taactacaac ggtaggacaa agtaaagttt ctttgatgga agaacgtatt aaattaatta
    14221 atccagattg taaagtcacg cctttacata tgttttatac agaagagaca tatgaagaaa
    14281 ttttcgataa ttacgatata gattattttg tagatgcaag tgatacaata atgtacaaag
    14341 tacacttaat gaaagagtgt cttgaccgag ggattaaagt gatttccagt atgggtgctg
    14401 caaataaaac agatccaaca cgttttacaa tttcagatat ctctaaaaca cataccgatc
    14461 caatggccaa aattattcgt caaaaattga aaaaactagg aattcgtaaa gggattccag
    14521 ttgtattttc agatgaaagt ccaattgtca taagagaaga tgtaaaagat atagttggag
    14581 ataaaaatgc tatcaatcga aaaggacaaa tgccaccttc ttcaaatgcc tttgtgccaa
    14641 gtgtagttgg attaatttgt gcaagttatg tggtgaatga cgtattaaaa gatattccag
    14701 ttcgtcgcat taaagacaaa gggcaataat taaaattgaa agtcataatt ttcaacgtaa
    14761 ggcaagtgta accataccat aaaactaaaa ctgactagtt cgcaagcatt gtagaatatg
    14821 cctcgtgtac tagtcagttt tgttttgatt atagctaaaa tatcaaaggt aatcgattta
    14881 atcttatttt tatggccttt gtttaagtaa attgttatat atttctttga gttgtcgttc
    14941 acttttagat gtcgtttttg gttcataata aattttgttt ttaagtttat caggtaaata
    15001 ttgctgtgaa acatagccat taacatattg gtgtggatat ttgtaaccaa tagatcggcc
    15061 taaatcttta gcaccttgat aatgtccatc ttttaaatga tttggaattt gacccacatg
    15121 accgtttcta atatcggaca atgcactatc aatggcactc attgctgaat ttgacttagg
    15181 tgataagcat agttcgatta ctgcttggct tagtggaatt ctagcttctg gtaaacctag
    15241 acgttctgct gattcaatag cagcaagtgt tctctgacca gcattaggtg aggctaagcc
    15301 tatatcctca tagctaatta caagtaatcg tcgaactatt gtaggtaaat ctccagcttc
    15361 aattaatcgt gctaaataat gtaaagcggc attgacgtcg ctaccacgga tagatttttg
    15421 gaaagcgctc ataacatcgt aatgcatgtc accatcctta tcacttacaa atgcaccttt
    15481 ttgtaagcag tctttagcat cttgcaatgt aacatgtcga taaccgtcct tgtcattatc
    15541 tgcgcttaat acagctaatt ccaatgcatt taacgcacta cgaacatcac cttgactttg
    15601 tgtagaaaag taggtcatgg catcttcatc aattttgggt tgatatgttt tcaaaccatt
    15661 ctcttcatct tctattgcac gagttaacgc ttggcgcaca tcttcgtcat ttaaaggata
    15721 taactcgaaa atttgcgctc ttgaacgaat cgctggattg atagcatgat aaggatttga
    15781 agttgtagca ccgatcaaga cgattttgcc attttctaaa tgaggtaata aaaaatcttg
    15841 tttggcttta tctagtcgat ggatttcatc taataacaag ataacttgac cagacatttt
    15901 agcttcttca acaacaagtt gcatatcttt tttagtgtta gttacagcat tcaattgtct
    15961 gaatttatat tgcgtactgc ccgaaattgc tttggcaata cttgttttgc ctataccagg
    16021 tggaccataa aaaatcattg aagttaattt ttttgtgtca accattcttc tgataatgcc
    16081 tcttggtcca actaagtgtt gttgggaaat gatttcatct atattttttg ggcgcattct
    16141 cgacgctaat ggttctgtac tcacatttgt cacacctttc ttatactatc ttaactcaaa
    16201 aaatgctaaa atgggaatat caatattcga attaagtgag gttatacaat gaaaatttct
    16261 actaaaggga gatatggact tacattgatg atttctcttg ctaaaaaaga ggggcaagga
    16321 tgtatatcat taaagtcaat tgctgaagaa aataatttga gtgatttata tttagaacag
    16381 cttgtaggtc ctttaagaaa tgcggggtta attcgaagtg tacgcggtgc taaaggtgga
    16441 taccaattaa gagtgccagc ggaagaaatc tcagcagggg atattataag actgttagaa
    16501 ggtccaatta catttgttga aagtattgaa tcagaaccac ctgcgcaaaa acaactatgg
    16561 attcgcatga gagatgcagt gagagatgtt ttagataata caacattgaa atatttagcg
    16621 gaatatgtag atacaagtga agatttagac ggatacatgt tttatattta accgtataaa
    16681 tactatacta taacataaaa acttcatatt ataatgttta gcgaacctcc tttgtggtat
    16741 ataaatatat acatctaagg aggtttcact atggcagacg aaagtaagtt cgatcaattt
    16801 aaaggcaatg ttaaagaaac agtaggtaac gttactgata acaaagaatt agaaaaagaa
    16861 ggtcagcaag ataaagcgac tggtaaagca aaagaagttg ttgaaaatgc taaaaacaaa
    16921 ataactgatg caattgataa acttaaaaaa taagttcaat taaactaatc aaatataaga
    16981 ggagcgatta atatgagttt tatagataaa gctaaagacg cagtagaaaa atttaaaaat
    17041 agcgataatg aacaagttaa aaatgtaaaa gataagataa atgaatatac aggatctaat
    17101 aacgaagaga aaaaagaaaa tgaagataaa gagaaataaa tttctttaaa ttttggatat
    17161 gattttcaat attttgaaag cgccccatca aataaacact tacacagtgt aaaattgaag
    17221 gggcgcttta atatttgcat tagaatttta agtgaatttg aatcattact aaaataaata
    17281 attgaacaat aaagacaact tttattcaaa atgctgttct gtttattttt attaatatta
    17341 tggatacata ctttcactta gtttatttat cacattataa agtgtttttt tcatttaact
    17401 gatttataac atttttgaat cgacgataag agtctaattg catatcttta tcataaatat
    17461 aacttatagc cataatttcg ttgacttcac cataagtttc cataaaatct tgaatttttt
    17521 gttgaacagt attttctgaa cctattaatg atattttctg tctttccata gccatcgcat
    17581 attctctcgg cgttaatata ttttgcagtt catgtgttgg cggttgaacg ggctgcattc
    17641 tgccacgagt gatactaacc attacttgtg ccatagatga agctaaatat tgtgcttcgt
    17701 catcattttc agcaacgatt gtatttaaac atacaataac ataaggttcg tctaatacat
    17761 cagaaggttc aaataacgtt ttgtagattt cgatagcttc tttcatttgt tgaggtgcaa
    17821 aatgtccagc gaacacatat ggaagccctt tgcgagcagc taaatgtgca gaatctgttg
    17881 aagaaccaag aatatataaa ggcacatttt tacctactgc tggataagca cgaacatatg
    17941 cttgttggtg agcggggccg aaatattgtt gtaataatga aacttcttcc ggaaatttat
    18001 aaacaccatc atgttgatct cgtcttaatg cacttgcggt catcatatct gttccaggtg
    18061 cacgtcctaa tcctaaatcg acacgatttg gaaataatgt tgccatcgtg ccaaattgtt
    18121 ccgcaacgat taatggagca tggttaggta acatgattcc tccagaacct acacgtatgt
    18181 gtttcgtatg ttctaacgta tgttgaatta ataaagcagt tgctgaactt actaggttgg
    18241 gagtgttatg gtgttcagca atccaatatc tttcatatga taattcgtct aaatgttgtg
    18301 cgagcttaac catatcatta attgctgttc gttcatcttc accttctcga atagggacaa
    18361 ggtttaatgc tgaatattta atcgccattt ttatatcgtc cttccttttt ttgatataag
    18421 aagtatacca atcgaataac cagataaata ttaaattgct cataaccaaa ataatagtac
    18481 gttacatttt acaaatgata aaatatcttc aaatactaaa atttataaaa aagacaagaa
    18541 taaagcgata aacttaaaaa gcctatcgaa aagcatgtgt agaaaatggt aaaatataat
    18601 taaaaacatt ataaataaag ttaaacagaa aagtagaaaa atagtatgta aggtgctatt
    18661 attggaacat tgataaaatt gtgaggagtg tccaaacatg gaaatttatg cagattatgc
    18721 tgcgaccaca ccagtaaaac ctgaagtagt agatgcgatg atgacgattt acaattcgca
    18781 ttatggtaat ccatcatcaa tacatgctaa gggaagagat gcgcgtaaat atttagatga
    18841 gtcaagacgt caaattgcac aattacttgg tgcagataca catgaaatta tatttacgag
    18901 tggcgcaaca gaatcgaata atacggcaat taaaggcata gtaaaagcaa atgaacaatt
    18961 gggtaatcac attatcacat cgaagattga acatcattct gtgttacatg tttttgaaca
    19021 attagagcga gaaggttttg atgtaacata tctagatgtt gatgatacag gtgctattga
    19081 tttagatcaa cttgaagaaa caattacaga taagacgatt ttagtatcta ttatgtttgt
    19141 aaataatgaa gttggcacag tgcaacaaat ttatgatatt caagatatta ttgctgagac
    19201 aaatgcatat ttccatgttg atgcggttca agcaattgga catcttgatg ttaagtttga
    19261 cgaatttgag atcgacgcaa tgagtattac tgctcataag tttggcggac caaaaggtgt
    19321 cggtgcatta ttagttaaag accatgtcac attagattat cctcaacttg gcggtgaaca
    19381 agaattgaaa cgccgtgcag gcactgaaaa tcttgcacaa attgttggga tggctaaagc
    19441 attacaatta gcagaaaaga atcgagacga taacaatatt catttaatga atttaaaaga
    19501 acaattttta gttaaattac aagaaagagc gattccattt gaattaaatg gttcaatgac
    19561 ggatgctaca ggccatattg taaatctata ttttccgttt gttgaagttg aaacgatgtt
    19621 aacattgttg gatatggcac aaatctatgt gtcgtctgga tctgcatgta cagcaggctc
    19681 tacacaaccg tcgcatgttt tagatgcaat gttcgaagat gaagagcgat caaatcattc
    19741 gattcgattt agttttaacg aattgactac tgaaaatgaa attaatgcaa ttgtagctga
    19801 aattcataaa atatatttta aatttaagga ggagtcataa ttgtcaaata aagatatacg
    19861 tgttgtcgtt ggtatgtcag gcggtgtaga tagttctgta acagcccacg tcttaaaaga
    19921 acaaggttat gatgtcattg gcatatttat gaaaaactgg gatgacactg acgaaaatgg
    19981 cgtatgtact gcaactgaag attacaacga tgttattgaa gtgtgtaatc aaattggcat
    20041 tccatattac gctgttaatt ttgaaaaaga atattgggat aaagtcttta cgtatttctt
    20101 agatgaatat aaaaaaggtc gtactccaaa cccagacgtt atgtgtaata aagaaattaa
    20161 gtttaaagcc tttttagatc atgcgatgaa tttaggtgca gattatgtag caacaggaca
    20221 ttacgcacgc atacatcgtc atgaagatgg tcatgttgaa atgttacgtg gtgtagataa
    20281 taataaagat caaacatact tcttgaatca attatctcaa caacaacttt caaaagtgat
    20341 gttcccaatt ggcgacatcg aaaagagtga agtgcgtcga attgctgaag aacaaggact
    20401 tgttactgct aagaaaaaag attctacagg catttgtttt atcggcgaaa aaaactttaa
    20461 aacattttta tctcaatatt tacctgcgca accgggtgat atgattacac ttgatggtaa
    20521 gaaaatgggt aaacatagtg ggttgatgta ttacacaata ggacaaagac atggtttagg
    20581 tataggtgga gatggcgatc cttggtttgt tgtcggtaaa aacctaaaag ataatgtttt
    20641 atatgtagaa caaggattcc atcacgatgc attatacagt gactacttaa ttgcttcaga
    20701 ctattcattt gtaaatccag aagataatga cttagatcaa ggttttgaat gtacagctaa
    20761 atttagatat cgccaaaaag atacgaaagt ttttgtgaaa cgtgaaaacg accatgcact
    20821 acgtgttact tttgctgagc cagtaagagc aatcacacct ggacaagcag ttgtttttta
    20881 tcaaggtgat gtgtgtcttg gtggagcaac aattgacgat gtgttcaaaa atgaaggtca
    20941 attaaattat gttgtttaag caatgacaac aataaattac ttatttgaag tttcagcgtt
    21001 ggaaatgacg aaagacagtt tttgatgaga ataattcatg atgatagagt ctgggacatc
    21061 acaatgtcct aggctctaca atgttatatt ggcgggcccc caacacagaa gctgacggaa
    21121 agtcagattt caataatgtg cgagttggcg gggcccaaca cagagaattt cgtaaagaat
    21181 tttaacaggc aatggaagtt ggggacgggc ccccaacata aaagctgacg gaaaatcaga
    21241 tttcaataat gtgcaagttg gcggggcccc aacatagaga atttcgtaaa gaattttaat
    21301 aggcaatgga agttggcggg gcccaacaca gagaatttcg taaagaaatt ctacaggcaa
    21361 tgagagttgg ggataacaaa ttaattttgt aaaaatatca tttctgtctc acccctatgc
    21421 atgagtctaa ttatgtattc ctattttaag tacataatag tggtggctaa tgtggaagaa
    21481 ccattacata ataaaccgtt aatggttctt tagcatttcc attccattcc cgctttttca
    21541 tgaatgaaga tgatattaga ttatatttta ttcgttgtta agtgattcga gacatacaat
    21601 ttatcaagat gttataattg atgagaaatg aggttcgtaa atgatagatc aacaaacaat
    21661 atatcaatac atacaaaatg gaaaaataga agaagcgtta caagcattgt tcggaaatat
    21721 cgaagaagat cctacaatta ttgaaaatta tattaatgcc ggcatagtac ttgctgatgc
    21781 gaatgagatt gaaaaggcag agcgtttttt ccaaaaagct ttaacaatag atccgaagaa
    21841 tggcgtcgta ttttataatc tagcaaatgt atattataat cagcaacgtt atcaagaatc
    21901 tattaaatta tatcaacaag cattacaaac agagattgaa caagttgatt gtaattatat
    21961 gatcggtatg gcgtttaatc agttagaatc atttaagctg gcattgccgt atttaatgac
    22021 tgctgcagaa ctagataaag acaaagatgc agaagttcaa tttcaatatg gtcttgtatt
    22081 atgtcaatta gagatgttta atgaagccat aactcaactt aaacatgtat taacgattga
    22141 taaaaatcat gttgatgcaa gatacaattt gggtttagcg ttatttatga aaaatgagaa
    22201 tattgatgaa gccataactc attttaaaga agctgtgact atcgacccta aacacttatt
    22261 aagtcagcat gcgctgaaaa cattcactaa aatgaaagag gaggagtaac atgtcagacc
    22321 ctacactatt tgattattca atgatcaaag gtactgttga agcgatattg tttcaaaaca
    22381 gtgataattt ttatacagtg cttaaagtag atactattga aacaaatgaa gactttgata
    22441 caatgccaac tgttgtaggg tttcttccga atattgttga aggcgatgtc tacacattta
    22501 aagggcaagt tgttgatcat ccacgttatg gtaaacaatt aaaagcagag acttttgaaa
    22561 aagaaatgcc acaaacgaaa gaagcaatta taagttattt atcaagtgat ttgtttaaag
    22621 gtgtaggtaa aaagacagct caaaa
//
LOCUS       DAMZPP010000028        22386 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMZPP010000028 DAMZPP010000000
VERSION     DAMZPP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22386
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.028"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            248..1372
                     /locus_tag="POH73_000839"
     CDS             248..1372
                     /locus_tag="POH73_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDI6886811.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRPLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLVVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(1463..2416)
                     /locus_tag="POH73_000840"
     CDS             complement(1463..2416)
                     /locus_tag="POH73_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HDI6886812.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKD
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKNNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(2775..3266)
                     /locus_tag="POH73_000841"
     CDS             complement(2775..3266)
                     /locus_tag="POH73_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6886813.1"
                     /translation="MKKLVTATTLTAGIGTALVGHAQHADAAENYTNYNYNTTQTTTT
                     TTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTVNHT
                     PSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGNYNY
                     IHI"
     gene            complement(3503..3925)
                     /locus_tag="POH73_000842"
     CDS             complement(3503..3925)
                     /locus_tag="POH73_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HDI6886814.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITFIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYVIPLTIRFI"
     gene            4173..5255
                     /locus_tag="POH73_000843"
     CDS             4173..5255
                     /locus_tag="POH73_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HDI6886815.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVESIGLDKLDLEKYLK"
     gene            5350..6750
                     /gene="nhaC"
                     /locus_tag="POH73_000844"
     CDS             5350..6750
                     /gene="nhaC"
                     /locus_tag="POH73_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDI6886816.1"
                     /translation="MMTRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     AWIAKRVGLTWQDLETGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLSISPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIVGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNDSMIHQSHISFSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVGEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            6924..7118
                     /locus_tag="POH73_000845"
     CDS             6924..7118
                     /locus_tag="POH73_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886817.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDAPKFGCVNGCMLFILY
                     YRVNRIGGNRSFEIFTNPIF"
     gene            complement(7187..7996)
                     /locus_tag="POH73_000846"
     CDS             complement(7187..7996)
                     /locus_tag="POH73_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6886818.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYSYNNYNNGYSYNNYSR
                     YNNYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSG
                     YTSGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQT
                     TQGAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            8606..10561
                     /locus_tag="POH73_000847"
     CDS             8606..10561
                     /locus_tag="POH73_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6886819.1"
                     /translation="MTDNYSLHIFKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDV
                     KRNDVTVIMLNDLYQIDGHDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQPYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGDTPKLIRKRNSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSTERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPNELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIARSKIICDVDLINENNDLKEKITMSDINKVTELLTNYTKFRGLIM
                     HMSIISEASKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            10587..11330
                     /locus_tag="POH73_000848"
     CDS             10587..11330
                     /locus_tag="POH73_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDI6886820.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDRLLKMKEIQSKMALLQMLSYLNNHQWLLKARDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELLLNLKCYLNMCQLSLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVKLNNK"
     gene            11394..11720
                     /locus_tag="POH73_000849"
     CDS             11394..11720
                     /locus_tag="POH73_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDI6886821.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGANEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSNNVEMFVEYV
                     KQHMIV"
     gene            12148..12495
                     /gene="sarR"
                     /locus_tag="POH73_000850"
     CDS             12148..12495
                     /gene="sarR"
                     /locus_tag="POH73_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HDI6886822.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
     gene            complement(12684..13520)
                     /locus_tag="POH73_000851"
     CDS             complement(12684..13520)
                     /locus_tag="POH73_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HDI6886823.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(13520..14134)
                     /gene="ureG"
                     /locus_tag="POH73_000852"
     CDS             complement(13520..14134)
                     /gene="ureG"
                     /locus_tag="POH73_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HDI6886824.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(14147..14836)
                     /locus_tag="POH73_000853"
     CDS             complement(14147..14836)
                     /locus_tag="POH73_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HDI6886825.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNNPWIAWYHQQMQDKKAKLNPAICFTMLGHYLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEETRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(14829..15281)
                     /gene="ureE"
                     /locus_tag="POH73_000854"
     CDS             complement(14829..15281)
                     /gene="ureE"
                     /locus_tag="POH73_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HDI6886826.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIVVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            complement(15294..17009)
                     /gene="ureC"
                     /locus_tag="POH73_000855"
     CDS             complement(15294..17009)
                     /gene="ureC"
                     /locus_tag="POH73_000855"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HDI6886827.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFPNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDYEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            complement(17006..17416)
                     /locus_tag="POH73_000856"
     CDS             complement(17006..17416)
                     /locus_tag="POH73_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HDI6886828.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGTENVNKKGGKRS"
     gene            complement(17430..17732)
                     /locus_tag="POH73_000857"
     CDS             complement(17430..17732)
                     /locus_tag="POH73_000857"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HDI6886829.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            17992..18897
                     /gene="yut"
                     /locus_tag="POH73_000858"
     CDS             17992..18897
                     /gene="yut"
                     /locus_tag="POH73_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HDI6886830.1"
                     /translation="MKMIDVLLKNISQVVLISNKWTGLFILIGLFVADWTVGLAAMIG
                     SIIAYAFARYINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHVIQSLFEGFSQVFIEASVIGGVCILIGILIASRKAALLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            19112..20266
                     /locus_tag="POH73_000859"
     CDS             19112..20266
                     /locus_tag="POH73_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDI6886831.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIGDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKEYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELKSVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKASNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYTAESQIRNATSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            20479..21387
                     /locus_tag="POH73_000860"
     CDS             20479..21387
                     /locus_tag="POH73_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6886832.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDIEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            21786..>22386
                     /locus_tag="POH73_000861"
     CDS             21786..>22386
                     /locus_tag="POH73_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDI6886833.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSKEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     RDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALES"
BASE COUNT         7703 a         3250 c         3931 g         7502 t
ORIGIN      
        1 ttcagtcaac tactgccaat ataatattat agaccctagg acattgattt atgtcccaag
       61 ctcccacatc attgttacaa atgtacatta tgtaaatgaa tattgctgtc taaatgtgca
      121 tgtaatatac aatggtgcag ataatacact taagtcttta aaaatgaaac gttagttcca
      181 agagtcattt ttaaacaata gtgcatgtga taaaatagaa aagaatgaaa aatatagagg
      241 tgacaatatg aagatagcaa ttataggtgc aggcatcggt ggattaacag ctgctgcact
      301 attacaagaa caaggtcata ctattaaagt ctttgaaaaa aatgagtcag ttaaagaaat
      361 tggcgctggg attggtatcg gagataatgt gcttaaaaaa ctaggtaatc atgacttagc
      421 taaaggtatt aaaaatgctg ggcaaatctt atctacaatg acagtgttag atgacaaaga
      481 tcgcccgtta actacagtta aattaaaaag taatacattg aatgtgacgt taccacgcca
      541 aactttaatt gacattatta aatcttatgt aaaagatgat gcaatattta caaatcatga
      601 agtcacgcat atagataatg agacagataa agttaccata cattttgcgg aacaagagag
      661 tgaagcattt gatttatgta ttggtgctga tggaattcat tctaaagtga gacaatctgt
      721 aaatgctgac agtaaagtat tatatcaagg gtatacatgc tttagaggtt taattgatga
      781 tattgattta aaacatccgg attgtgcaaa agaatactgg ggaagaaaag gaagagtagg
      841 tattgttccg ttattaaata atcaagcata ctggttcatt acaattaact cgaaggaaaa
      901 caatcataaa tatagttcgt ttggtaaacc tcatttgcaa gcatacttta atcactatcc
      961 aaatgaagtt agagagatct tagacaaaca aagtgaaaca ggtatcttat tgcataatat
     1021 ttatgatttg aaaccactca aatcttttgt ttatggtcgt actattttac taggagatgc
     1081 agcacatgcg acaacgccta atatggggca aggtgctgga caagcaatgg aagatgctat
     1141 cgtattagta aattgtttta atgcatacga ctttgaaaaa gcattacagc gttatgataa
     1201 aatacgtgtc aaacatactg caaaagtaat taaacgttct agaaaaatcg gtaaaattgc
     1261 ccaatatcgt agtcgtttag ttgttgcagt tagaaatcgt attatgaaaa tgatgccaaa
     1321 tgcattagca gctggacaga ctaaattctt atataaatcg aaagaaaagt aatacaacaa
     1381 tatgaaaacc ccgtatgttg aaattagagc tcaacatatg gggggtcttg tttttataat
     1441 gtcattataa taaattcaat tattagttaa cgacaaattg tggtttctca ccttgaacgg
     1501 cactaattgc agcattagca acaattttag acatcatgtc acgtgcttca aatgtagcat
     1561 taccaatatg cggtgttaat actacattat taagtgattt taagtcatcg gtaatgtctg
     1621 gttcgaattc atatacatca agtgcagcac cttcaatttc attatttttc aatgcttgca
     1681 ctagtgcttg ttcgtgcacg attggaccac gagaggcatt gattaaatac gccgtagatt
     1741 tcatcatttt aaattgttct gtatcaatta aatgatgcat tttaggattg taagcagcgt
     1801 tgatagtgat aaaatctgca ttctttaata gtgtatctaa atctacatat tttgcaccga
     1861 tttctcgttc tttttcttct ttgcgattag gtcctgtgta tagtacatcc atgtcaaatg
     1921 ctcttgcacg acgcgctact gcactaccaa tttcacctaa accgataatg ccgattgttt
     1981 tcccagatac ttctctacct ctgaaaaata aaggtgccca tccgtcaaat ccagttgtac
     2041 gtgataattg gtcgccttca acaatacgac gcgctactgc aagtactaat ccaattgtta
     2101 aatcagcagt cgcgtttgtt gatgctttag gtgtgtttgt aacatctata cttttttctc
     2161 gagcatactc gatatcaata ttattaaaac cagcaccata gttggcaatg atttttaagt
     2221 ctttaccagc atcgataaca tctttatcaa cgtttgtaga taataaacta attaaggcag
     2281 tcgcatcttt aacaccttta attaaagtgt ctttatcgac taatccttta ccttcataca
     2341 tttcaacttc aaaatgttct tgtaaaagtt ttaaacctac ttctggtatt gcaccagcaa
     2401 cataaacttt ttccataaaa gatcactcct tttatcttag tatagtagaa gattagacag
     2461 tatacaacta tgtcatgatg tcttgtgtat caatgatgta agcgtgtact tttgatggag
     2521 gtgatataac ttagtcactg tagaactatg aatattgtaa tgtggaaaac tggatcaatt
     2581 aaattagata acgtagtttt aaagttaata gtattgaaaa aattaatatt atgaatatgg
     2641 gaggaaatat atataagtag gtggcaatga aaaatagcaa aaaaagagct tctcctataa
     2701 aggaaagctc aaagtttttg atgacatatg tactagaatt aagtttcaag acaatatgta
     2761 tcatcgtgtt taaattaaat atggatgtag ttatagttac ctgcttcact tgcagaaata
     2821 gttcttgagc ttactgagaa aggtccgcca ctataattca tttctgaaat tgtaactgaa
     2881 ccatcactgt ttacactttc tacatatgca acgtgaccaa atggtccttc agaagattgt
     2941 aggatagcgc ctttagaagg tgtatggttt actgtgaatc cagcaccttg tgcagcagca
     3001 gcccaattat tagcatttcc ccaagtagat ccgatttctc cgcctacttt atcatataca
     3061 taccaagtac attgtcctgc agtgtataag ttaccagaat gtgaaattga tgatgtagtt
     3121 gtcgtagttg ttgtagtcgt tgtagtttga gtcgtattat agttataatt tgtataattt
     3181 tcagcagcgt ctgcatgttg tgcatgacct actaatgctg taccaattcc tgctgttaac
     3241 gtagttgctg ttactaattt tttcatgaat aaagtcctcc aaagttctat atcttttttt
     3301 ataaataaaa cgtagcgact gttttattct cacatctcga attgatgaca atagttactt
     3361 taacaaaatt aatgcttctt gtggggaatg ttattgattt gtaaaagaat aaaaaaactt
     3421 tgactaattt tgtaataaaa attagtcaaa gttacagtga gattaacaga taattaatag
     3481 gaaatattta tttgtaatat gtttaaataa atcgaattgt taaaggtatt acatattctt
     3541 ggccattata atatttgaca cacgcaataa ttgtgaatac aaaagataaa attgagaaag
     3601 cgaatatgga taaaataccg ataaacgtaa tgatgaaacc tataatgata atgaaatcaa
     3661 tatcggtagc aattaggaaa acgcctataa aagtgataac gactaaaacg atagaccaaa
     3721 taatataaga aatcgtatag ttaagataat tttttccagc acgatcaact agtttcgatt
     3781 catctttttt caataaccat ataatcagtg gaccaataat agatgtgaat aaacttaata
     3841 aatagataag catcgccata atgttctcat cattggattt gcgattcggt tgatgatttg
     3901 ttacgtcgtt catttcagtt gtcatattag acactccttt gaaaattgta atattatctt
     3961 taactataac aaaatataat caaaaataaa catgtttatt aaacaattat taaaaataaa
     4021 aataattggt ggacgtcggc gtttaaatag gttaatttaa ggttatatat acttaacatt
     4081 tataatgatg cgtaatgaat tcgcatcatt tttatattgt cttacgtata atttgttttt
     4141 aattttaacc aaagatagaa agagggttgt ttatgaaaat agcaattgta ggatcaggaa
     4201 atggcgcagt tacggcagca gtagatatgg tgagcaaagg ccacgatgtt aaattatatt
     4261 gtcgtaatca atctataagt aagtttcaaa acgcaatcga aaagggcgga tttgatttta
     4321 ataatgaagg tgatgaacgt ttcgtaaaat tcactgatat tagtgatgat atggaatatg
     4381 ttttaaaaga tgctgaaatt gttcaagtga ttattccatc ttcatacata gaatattatg
     4441 ctgatgtaat ggcagagcat gtaactgata atcagttgat attcttcaac atggctgcag
     4501 caatggggtc aattcgtttt atgaatgttt tagaagatag acatattgaa acaaaaccac
     4561 aattagcaga agctaatacg ttgacgtatg gtacgcgtgt cgattttgaa aatgcagcag
     4621 ttgatttatc tctaaatgta cgtcgtatct tcttttcaac atatgataga agctgtctaa
     4681 atgattgtta tgacaaagtt tcaagtattt atgatcattt agtaaaagag gaaagcttaa
     4741 ttaaaacaaa tcttgaaaat ggtaatcctg aagtgcatcc agggccaaca ttattgaatg
     4801 tcggtcgtat tgattatgct ggtgagttcg ctttatataa agaaggaatt actaaacata
     4861 cagttagatt acttcatgca atcgaattag aacgtttaaa tttaggccgt agattaggtt
     4921 ttgaattatc aacagctaaa gaatcacgta ttgaacgtgg ttatttagaa cgtgataaag
     4981 aagatgaacc attaaatcgt ttgtttaata caagcccagt attttcacaa attccaggac
     5041 caaatcatgt agaaagcaga tatttaactg aggatattgc atatggttta gtattatggt
     5101 caagcttagg tcgtgttatt gatgtaccga caccaaatat agatgcagta attgtcattg
     5161 catcaaccat tttagagaga gacttctttg aggaaggctt aacagttgaa tccattggct
     5221 tagataagct tgatttagaa aaatatttaa aataaatgat ggcttgaaga tagaaaagga
     5281 tatagcatta tgcaaaagca ataaattgaa gaaaagaggt ttctcatcaa taagcgaagg
     5341 ggacgataga tgatgacaag aaaaccaacc tttttagaat caatttcgac aatgattgta
     5401 atggttattg ttgttgtaac aggctttgtg ttttttgata ttccaattca agtattatta
     5461 attattgcct cagcatatgc cgcatggatt gcaaaacgtg taggcttaac atggcaagat
     5521 ttagaaacag gcattgcaga acgtttaaat actgcaatgc ctgcaatttt aattatacta
     5581 gcggtaggaa ttatagtagg cagttggatg ttttctggca cagtgccagc cttgatttat
     5641 tatggcttag atttattgaa tccaagctat tttttaatat cagccttttt tataagtgct
     5701 gttacatctg tagcaactgg tacagcatgg ggctctgcat caactgcagg gattgcactt
     5761 atttctatcg gtaatcaatt aagtatctca ccaggaatgg ctgcgggtgc tattatagca
     5821 ggcgctgtat ttggcgataa aatgtcacct ttatcagata caactaattt agcggcgctt
     5881 gtcactaaag tgaatatatt taaacacatt cattcaatga tgtggacaac aatacctgcg
     5941 tcaatcatcg gtttattagt atggtttatt gttggatttc aatttaaagg gcattcaaat
     6001 gataagcaga ttcaaacatt gttatcagag cttgcacaga tttatcaaat taacatatgg
     6061 gtctgggttc ccttaattgt gatcattgtt tgtttgctat ttaaaatggc tacagtgcca
     6121 gctatgctaa tatcaagctt ttctgccatt atagtgggga cttttaatca tcatttcaaa
     6181 atgacagatg gtttcaaagc aacatttagt ggttttaacg actcaatgat acatcagtct
     6241 catatttcat tcagtgtgaa aagcttgtta gaacagggtg gtatgatgag tatgacccaa
     6301 atattagtaa cgatattttg cggatatgca tttgcaggta ttgtagaaaa agcaggatgt
     6361 ttagaagtct tattaactac tatttctaaa ggcatccatt ctgtaggaag tttaatatgt
     6421 attaccgtta tttgttgtat tgcgcttgta ttcgctgcag gtgttgcttc gattgtaatt
     6481 attatggtcg gtgtgttaat gaaagatttg ttcgaaaaat accaagtctc tcgttccgtt
     6541 ttatcaagaa cacttgaaga ctcaagtacg atggttttac ctttaatacc atggggaaca
     6601 tcaggtattt actatacgaa tcaacttcat gtctctgttg gagaattttt catatggaca
     6661 gtaccatgtt atttatgcgc aattatagca attatctatg gttttacagg aataggtatt
     6721 aaaaagtcat cgaattcacg tttaacttaa tgtgagcgtg gaatatatat aatatgttga
     6781 aacactttaa tcatttataa ttgtagcggt tataatttga aaaggtttta acttagaata
     6841 aatatcctct atacatatac tgaatatgtt ttgtagcgga acatgttaat atatgtaatg
     6901 taagctttat gtcatggcat gtaatgacta aattaattga gaatttgaag gcaagtatat
     6961 ttgtaagtac tttaactaaa aatttatcaa tgtatagccg atttgacgcg cctaaatttg
     7021 ggtgtgtcaa tggctgtatg ttgtttattc tttattacag agtgaatcgg attggtggaa
     7081 atcgaagttt tgagattttt accaatccga ttttttgatg tctagaaatt aaaaaagcca
     7141 acaaggctct tgaaaccttg ttggcttaat tcactttcat cacaaattag tgaatgaagt
     7201 tataaccagc agcttggcta gctgagattg tacgtgaagt tacaacacct gggccataac
     7261 catagttcat ttctgaaact cttactgaac cattgctgtt aacactttca acgtatgcaa
     7321 cgtgaccgta tgcaccttga gttgtttgca taattgcacc agcttttggt gtattgttca
     7381 ctgtgtaacc agctcttgca gctgcgttag cccagttact tgcattgccc caagttgaac
     7441 cgattttacc acctacacga tcaaatacgt agtatgtaca ttgaccagaa gtgtataagt
     7501 tacgtcctga agtataacca cttgagattg aacggccatt tgatgatgga gccatagttg
     7561 tagttacttg aacattgttg cttgaagtgc tgtagcttgc acctaaacca ccagtacggt
     7621 agctgtttgt gttgtaacta ttatagttgt tgtagttata tgattgatta ttatttgagt
     7681 agttgttgta acggctgtag ttattgtagc tataaccgtt gttgtaattg ttgtagctat
     7741 agttattgta gccattgtag tagtaatagc tgtagtagcc attatcttgg tttaattgac
     7801 ttggatgcca gttacctttc catgtgtaat ggtagttacc ttgtgcatca atagtgtaag
     7861 tatagctata tgatgttggg tcatttggat tataaccgta gttatcttgc tcagaagcat
     7921 gagcttgatt tcctgatgca attgcgattg tagcgaatcc tgcagttgcg atagtagctg
     7981 tagcgatttt cttcatttta aaaatatcct cctaaaaatt ttaaatctaa aatattttcg
     8041 taatgtccgt gtgacaaaat taatgttata agttatctct cgtaattaaa cgacaagaaa
     8101 gactataaca gaaattagcg tccttgtgtg ctttgttaac gttttgtaat tttttgctaa
     8161 tatcttgaca caatagaatt gtaaaagtat agaaatttgc attttgcaaa acttataact
     8221 acggcattct ttgtaaaaac tgaatgtttc gaaaataagt atgttacaaa tttgtaatat
     8281 tactgaaaat tctaaatgta tattttgtgc ataatatagg acttttaatc agaaaatgca
     8341 taaaaattcg acacgaacga tcaaatttct ttggttagga aagctttggt atttggataa
     8401 aaatgtatat ttaatggata aaatatccta atttagcata aaaaaatgtt ttaataaaag
     8461 tattatttga tataatcgat ttatgttttg ttactgctaa aaaacatgtg gcgatataat
     8521 tggtagtgaa tattagatgt tgacaattga tttgagaaag acaaacagga gtaaatcaat
     8581 atatttgtat atgtgaggat gtaagatgac agataattat tcactacata tttttaaaag
     8641 tatgaaaatg agtagtgtga aggaagcaaa cttaaaaatt atatattggc ttcaaggtac
     8701 aggtgtagtt tcaatcaatc tacaacaata tgatgtgaaa agaaatgatg tcaccgttat
     8761 tatgttgaat gatttgtatc agattgatgg tcatgatgat agtgtatgtt gtgttgttga
     8821 agtaagtgca aagacatttt tgaagtttat gaatgcaaac tatatgatgc gtggcaagat
     8881 attaacaaat gaggttgcta gcacttttag aatcttgatt aagtatttaa tctacagcaa
     8941 aattaaacaa cagccttata atgctaatga taagattatt aattatatgt gtgttgaatt
     9001 aatgtcatta aatacatacg atggtagtcg tcatttagta gccgaagaag tgcacgatta
     9061 tttaacaaac aaccaccata aaaagataaa taggaaagat gtcattaatc aagttagtat
     9121 cacaaacaaa gctttaagtg acatgtttaa agcgacgcct tatagtaatt ttattcaata
     9181 tttaaatcat attagactgg agcattgctt aattgatatt ttaacttcta agaagcccat
     9241 tgaagaaatc gcaagtagtc atggttttaa tcattattct cgttttatac acctctttaa
     9301 agaaacgtat ggtgacactc ctaaattaat tagaaaaaga aatagtccaa catctcattc
     9361 aattaaccat tcccaattag ttgaaattga taaaaatata attgaattat ttgatgatac
     9421 taatcaaagt tcttcaagta tgcgcgagat tgatatacct tttgagccta caaaaaggag
     9481 tcaaatgtat caacctaaaa acatctatat taaaggaagt aatttttatt ggatggatta
     9541 ctatactatt aaacaaatca ttcaaagatt aggtgttaat cctgaaaact tgcatataat
     9601 tgttacaatc gacacaagca atgagctatc gattgaacaa atcactttat tacttcaaaa
     9661 gatagtgaat tacaatgtaa atgtagtatt tcgtattaaa catgaatata aggaattgtt
     9721 aacgagtact gaaagaggca agatagaaaa gctagtagct acaattttta atatgacttt
     9781 tgagagtaat agatgtaaga ttgcattttt aattaacgac ttaaatacta ctgcaattca
     9841 aaaactgaaa cgtttaatag ataattatat aggagtattt gaattaatat atcatctgga
     9901 accaaatgag ttgaatacgc gctatgataa agaaattgat catctaatag actattttat
     9961 cttaccgcta agacaaatag aacaggtgtc tattgcacgt tctaaaatta tatgtgatgt
    10021 agatttgatt aatgagaata atgacttaaa agaaaaaatc acaatgtctg atattaataa
    10081 ggttactgaa cttttgacaa attatacaaa atttcgagga cttattatgc acatgtcaat
    10141 tataagtgaa gcgagcaaag aaataaattt gactagatta tcaatattca tttttatcat
    10201 aaatatactt aatcaattac gagggattgt tatttatcaa aatgacacga tgattgtaac
    10261 gaagttcaaa catgaagtac aatgtgtcat ttgtttgcca gccgcattgt taaatgagaa
    10321 ttcagacgct atacgtttat cgtgtaaggg tcttcaacaa tttagccatg ctaaaactaa
    10381 acaaattgtt ttaagtatag ataaagataa tcatacttcc gaaaatggtg tacttgtgaa
    10441 tgatattatg caacaacaca gttattggaa agctaatttt atagaaagaa atatttatga
    10501 tcattcatta atggtctcaa gcaatagtat tgttcatttg aaatatcatc ttgagatata
    10561 ggcatggttt aagagaggtt ttaaagatgt ctaaatttaa agttaagcgt ttaactgcac
    10621 acatactaat gttgaaagtc attaatgata tgttgaaata ttcatttcat ctcactttga
    10681 ctcaagtgaa actattaaat ctattagtta aatatgataa ttctgattcc aatagcgctt
    10741 caatagatag gttattaaaa atgaaagaaa tccaatctaa gatggcactt cttcaaatgt
    10801 taagttattt aaacaatcat caatggctat tgaaagcaag agatcaaagg gatcaacgta
    10861 aattgactat ttcgattaat aaaatgcata tagataaaat agaatatatg aataatgagc
    10921 ttagtgacta tattgaacgt tattttggtc aatatacatt tgattttagt tgtgactatg
    10981 tatcgtattt attgtcgtca caagaattat tgctaaatct aaagtgttat ttaaatatgt
    11041 gtcaattatc attagaagag ttatacgttt taggaatatt aaatctgcat aaaggtcaat
    11101 tgacggttaa agaattacag ggtgaattcc atcacccgat atttgcggtt agtccgattt
    11161 taaagtgttt aattttgaaa ggattagtta aaaaagagag atgtgaatta gatgaaaggc
    11221 gtgttattgt aacaatcaag cgggaaaagt tttctaaagt cactatgctt attcaagcat
    11281 gttataatta tcttgaaaag ggaatacaag ttaagcttaa taataaatag ttaatcactt
    11341 gtatttcata tacctatgaa gaatagttta tagttttaag tgggagggat attatgattt
    11401 tagataatgt gaatccaaat gatttatttc caactgaaaa aaagggtccg tcagtgttag
    11461 gaataattga gtatcaagtt caaggtgcga atgaatttga aggggcattt atagcaacaa
    11521 atgaaagatt aattatgaat gtagatatga atggccaatt ttattataga agtattagtt
    11581 ataatgaaat agaaaagata gattatgatg gtcaaactat aatgttcaaa tttaatattg
    11641 gtaatgtccc aatgcatgat attaaaagta ataacgttga aatgtttgtt gaatatgtaa
    11701 aacaacatat gatagtataa tgtcaatgta tgtaggttga taagaagagt ttgaatgata
    11761 actcattgca tttaaagata aatggcatgc gttatgattt aaagctcttc tttttttata
    11821 ttttataaat tttgtgagca agccatccaa ttaatataaa aaatatcaaa tcaaacacgt
    11881 tgatatagat taaaatttta aaagtttaca tatcagttaa gatacaaaat attcagacta
    11941 ataattttca atttgacaaa atatcttaaa catcaaatta ttataagaaa taaatgtatt
    12001 taaccatatt ctagtaataa aaatattgaa tttttatact tatttgttta gaatgaactt
    12061 tataacatag ttggatagag tttcgattta ataaattaca tgtgaacctt gctacaacaa
    12121 gatgtgcatc agaggagtgg tttaataatg agtaaaatta atgacattaa tgatttagtc
    12181 aacgcaacat ttcaagttaa gaagtttttc agagatacaa aaaagaagtt caatttgaac
    12241 tatgaagaaa tttatatttt aaatcatatt ttaagaagtg agtctaacga aatctcatct
    12301 aaagagattg ctaagtgctc agagttcaaa ccttactatt taactaaagc tttacaaaag
    12361 ctaaaagatt taaaattgtt atcaaagaaa agaagtttac aagacgaaag aacagttatt
    12421 gtttatgtta cagatacaca aaaagcaaat attcaaaaac tgatttcaga attagaagaa
    12481 tacattaaaa attaaatcaa ggttaattgc gtttaataac attgaacgat aacaatttat
    12541 taaaacgaag ttatttattc agcattggga cataaaatta acttaaaatt taaatattga
    12601 agatgcttta attaaagtta aagactagcc ataccttatt tcagcttatt aagcttgaaa
    12661 caaggtacac tagtcttttt attttaatat tttcttagaa aatcaagttt acgatcataa
    12721 atattttctg cgatatagct ttggatggtt ccaagtattt tctctataat ttgtgtgcga
    12781 taagcaaaaa ttctaactgc aaaaccatgt gtaggcaatt gagaaatagc aacacgacaa
    12841 tcgaatgtat tgctgtaaga actaatggtt tcataaactg aatcgattag tttttggtta
    12901 acatcttcgt gtatgaagta agcagagcca taatgagagt aatgttccat ataacctatt
    12961 tcattgatcg attgtttatt aggattcaat aataaattat catatgtgac taactcatct
    13021 tcaatataaa tttcatttat taaatgcata tattgatact tgaaggcttc gccagttttt
    13081 gaataaccag gagttaaaat atcagtataa aataatgaac tagaattatt gagattgaac
    13141 gtattgtgtt gataaaattt agcattttca tatgcgatga ttggatcagc gacatattct
    13201 aaatatgcgt tatctttcaa attaaaagtt tgatactgct caacatgatt ggaaggtgtc
    13261 ttgtatattt ttgttgcacc ttgagatgtc aatgtcactt tagcgttatc ttcgacattc
    13321 acattcatac ggtaacgatc tccatctaaa tagccaccac ctacatttac tatataaaat
    13381 gtaggaatgg tagattgatt tagataaact ggacgtatca ctttaagtgc tttttcaaag
    13441 aaaatatctc ttgacactga tcgattgcca tcaaaaaaca ctgttaaatc aagttgccca
    13501 gtccattgtt gttcatccat tatgataatc ctttgagtaa agtgtcgcgt tcaatccaat
    13561 cgataacttc atccaaacct tcgtcggttt ttaagttagt aaaagtaaat ggacgtttac
    13621 cacgaaatac tttagtatct tcagccattt gttctaatga tgcaccaaca tagggagcta
    13681 aatcagtttt gttaattaca aagaaatctg acttaatcat accttgacca cctttacgtg
    13741 gaatcttttc accttgagca acatcgataa tataaattga aaagtcaaca agttctggac
    13801 taaatgttgc tgctaagtta tcgccaccag attctatgaa aattagttct atatcgtcat
    13861 gacgttctaa taattcgtct attgctgcaa agttcataga tgcatcttca cgaatcgcag
    13921 tatgaggaca tccaccagtt tcaacaccaa tgattcgact ttcaggtaga actcctgaat
    13981 ttactaatat cttttcgtct tcttttgtat atatatcatt tgtaataacg ccgatgctca
    14041 tttcttttga aagacgtttt acaacttttt caattaattg tgttttacct gcacctacag
    14101 gaccaccaat accaatttta atcggatttg ccacaattat aacctcctat gatatgaaaa
    14161 ttctaacatt gacattctca tgcgccattt gatttagttc taaaccaggc gctgtcatgc
    14221 caaaatctgc ttcttttaat tcgaaaatct gctttcttgt ttcttctata taaggaatca
    14281 tgtgagtaac tatcttttga ccagcagttt gtccaagtgg aatagcacga acagcatttt
    14341 gagttaaact tgaaacattt tgatataaat agtaatcaat aatcgtttca atatctacac
    14401 ctaaataatg gcctagcata gtaaaacaaa tagctggatt taactttgct ttcttatctt
    14461 gcatttgttg atgataccaa gcaatccatg ggttattata aagttctaaa gccaatttaa
    14521 ccatgcgagt ccccatttgt tttgcaccaa cacgtgtttc tttaggtaag ttttgaacaa
    14581 acatcagctt atctatgtgt aatacttttt gtgtatcatc attttccaat gcatcataaa
    14641 ctaaacgcat ggctaaacca tcagaatagg taagttgctc ttgtaaaaac atttttaacc
    14701 aagcaataaa agtatgatca tcatgaatta tatttcgttg aatatatgtt tcaagaccaa
    14761 atgaatgact gaaagcacct gttggaaatt gtgaatcaca gaactgaaat aatcttaagt
    14821 gtgtatgatc aatcatgaga atgtcctata tgtctaaaag ccttattaac tttacggtct
    14881 tctcgaacat atgggatgcc taaacttttt aataaatctt caactaaata atcatattgt
    14941 actagcattt cagtctctgt aaattgtgct ggcaaatgac gatttcctaa ttgatgggct
    15001 atatctccca tttcttgcaa tgttcttggt tgaatcacta aaagatcttc tgaattaaca
    15061 tccacaacaa tcatattatg gtcatctgcg tataaaatat ctccatattg taagtcaata
    15121 ggttgtttta aacgaatgcc aatttcagtg ccatgatctg ttacgactct ttgaatacgt
    15181 ttaacgagat ctgaattttc aaggtatact ttttcgacgt gcttttgttt ttctgaattt
    15241 gacaaattgg caatattgcc ttggatttct tcaacaatca ttctatgttc ctcctagaat
    15301 aagaagtatc tttgagttaa tggtaactca gttgctgcat tacttgtaat tttttctcca
    15361 tctacatata cttcatatgt ttgtggatca acgtctaatt taggtgttgc gctgttattt
    15421 ttcatatctg ctttagataa ttgtctaata tttttaactg gacgcaccat gcgttttaaa
    15481 tttaatgcac gattgatacc attttcataa gcagttttag acacgaatgt cattgacgta
    15541 cttgtaaggt ttccgccgta ttgaccatac attttacggt acttcatcgg ttcagatgta
    15601 ggtatagaac catttgcatc accatttacg gcagagttaa ttaatccgcc ctttacaact
    15661 aattcaggtt taacaccaaa gaaaattgga tcccataaga caatatcagc tagtttgccc
    15721 ggctcgatag atcctacata ttcagaaata ccatgtgtaa ttgctgggtt aattgtatat
    15781 ttagcgatat aacgtttgat acgattatta tcattatgtt cataatcacc atctaaagga
    15841 ccacgttgtt ctttcatgcg atgtgctact tgccatgttc gtgtaattac ttcacctaca
    15901 cggcccattg cctgtgaatc ggaactaatc atactgaata cacccatatc ttgcagaaca
    15961 tcttcagctg caatcgtttc tttacgaata cgtgaatctg cgaatgcgat atcttcagga
    16021 atagccgcat ttaaatggtg agtaatcatt accatatcta aatgttcatc tacagtatta
    16081 tgtgtataag gcaaagttgg atttgtagat gaaggtaaaa tatttggaaa tgcagcggat
    16141 ttaattaaat caggcgcatg accgccacca gcaccttcag tatggtacat atgaagtaca
    16201 cggtctttaa cagcagccat tgtgtcttcc ataaatcctg cttcatttaa agtatctgca
    16261 tgtaatgcaa tttgaacatc aaattcatca gcaacatcta atgcatgact caaagcagat
    16321 ggtgttgcac cccagtcttc atgtactttt aatccaattg ctccggcatt gatttgttca
    16381 atgagtgcag ttggatttgt tgcttgtcct ttacctgtaa aaccgacatt aatcggtaaa
    16441 ccttcggcag cttctaacat tctatgaata tgccatggac ctggagttac agtagttgct
    16501 ttagaacctt ctgaagcacc agtaccacca ccaatatgag tcgtaatacc actttctaat
    16561 gcgacttctg cttgttcagg attaataaaa tgaacatgag tatcaatacc accagcagtg
    16621 acgattttac cttcagcggc aatgatatct gttgttgaac ctataataat gtcgacatta
    16681 tccattatat ctgggttgcc ggcattacct atggcgaaaa tataaccatt tttaatgcct
    16741 atatcagctt taaccacttt atcgtaatcg ataataacgg cattagaaat gacaaggtct
    16801 gcaacgttca catcatcacg tgttacacga ggattttgcg ccataccgtc tctaatagat
    16861 ttaccaccac caaaagtagc ttcttcacca taaaccgcat agtctttttc aatttgagca
    16921 aatagattcg tatcacctaa acgaatagaa tctccaacag ttggaccgta taagctcgta
    16981 tattgatttt gcgtcatttt aaagctcatg atctttttcc tcctttttta ttcacgtttt
    17041 cagtaccgtt atctccgaat acgcctgcat attcatcatt ttcatcagtt gggcgataga
    17101 cacgtgactc atcgatagga ccattgacca taccacgaaa accaaaaatt ttacgtttgc
    17161 cagcatattc aactaattga acttcttttt tatccccagg ttcaaatcga acagctgctc
    17221 cagctggaat atctaaatgt tttccatatg ccatttcacg ttcgaaatct aatgctgcat
    17281 ttgcttcata aaaatgaaaa tgtgagccca cttgaattgg tcgatctcct gtattttcaa
    17341 cttcgataac tgtttcagga tgatggttat taatttcaac ctctgtactt tttgtaataa
    17401 tttctcctgg tatcatttga ctgcctcctt taaacaatag ggtgatgtac tgtgattaac
    17461 ttagtaccat cggggaacgt agcctcgatt tcgatatctg taatcatgtg ttcgacacca
    17521 tccatgacat cttctttgtt tagaatttgt ctaccataac tcattaactc tgcaacggtc
    17581 ttaccatcgc gtgcaccttc taataattca tcgctgatta aagctaatgc ctcaggatga
    17641 tttagtttca aaccacgtgc tttacgacga cgtgcaactt ccgccgccac tacaatcatt
    17701 aatttgtctt gctctcgttg tgtaaaatgc aaattaaaac ccccaatttc atattagata
    17761 caatttacaa aatttatatt aatcctaatt gttgtgataa acaagtaata tacaaagttt
    17821 aatacggaac agcaaaaaat aaatttacta tttcgtattt aaaaatatat gattatgatt
    17881 aaaacgaata gcaaaatata tttaggattt agaaaataga gtttttaata taactttttg
    17941 taaaatggta gtatattaat gagtaaacat tcaagaaaaa gggtgggcat tttgaaaatg
    18001 atagatgtgc ttttgaaaaa tatatctcaa gtagtgttaa ttagtaataa gtggactggt
    18061 ttatttatct tgattggatt atttgtggcc gactggacag ttggattagc agctatgata
    18121 ggtagtatca tagcttatgc atttgcacgt tacattaatt atagtgaagc agagattaat
    18181 gatgggttag ccggatttaa tccagtgtta actgccattg cgttaacaat ctttttagat
    18241 aagtcaggat tagatattgt tataacaatg atagcaactt tattaacatt accagtcgct
    18301 gctgcagtga gagaagtttt aagaccatat aaagttccga tgctgacgat gccttttgtc
    18361 attgtgactt ggtttacaat tttactttca ggacaggtta aatttgtaga tacatcgtta
    18421 aagttaatgc ctcaaaacat tgaaacggtt aattttagca acaatgatcg aatacatgtc
    18481 attcagtcat tatttgaagg attcagtcaa gtatttatcg aagcgagtgt aattggtggt
    18541 gtatgtattt taatcggcat attaatagca tcaagaaaag cagcactctt agctgttata
    18601 gctagtttgt taagctttat cattgtagct ctattaggtg gtaattatga tgatattaat
    18661 caggggttat tcggttataa ctttgtatta atggcaatcg cactaggata tacatttaaa
    18721 acagcgatta acccttatat ttcgactttt ttaggtgtgt tattaactgt agtggtgcaa
    18781 ctaggtacaa caacattgct tgaaccgttt ggcttacctg cattaacatt gccatttatt
    18841 attgtgacat ggattttatt atttgcgggt attaaacatg acaaagtaga tgcttgatag
    18901 ttaaatcaaa cctagtattg tttgaatatc accttaaacc atacagcgaa ttgtatagtt
    18961 taaggtgtat ttttggagaa aattaattgg atacttaatc gaattatcag ctttctttta
    19021 attgttcaca gcacgttcct ttttattttt aaagtcgagt gttaaactag gaataaataa
    19081 tttatattgt gtgatgtgtg gggtgactaa tatgaatgat atggataatt cctttttaat
    19141 aacaacggaa attcaaagaa aatggattga aaaattcaaa gtaattggag atacatttaa
    19201 ggctaaggct gaatataatg atcaacatag ccaatttcca tataaaaata ttgaatggtt
    19261 aattaaagaa ggttatggaa aattaacgtt accaaaagaa tatggtggtg aaggtgcgac
    19321 catagaagac atggttattt tgcaatcatt tttaggcgaa cttgatggtg ccacagcatt
    19381 atctattggt tggcatgtga gtgtcgtagg acaaatttat gaacagaaat tatggtctca
    19441 agatatgttg gagcaatttg ctgttgaaat taataatggt gcattagtta atagagcagt
    19501 tagtgaagct gaaatgggta gtccaacaag agggggaaga ccaagtacac atgctgttaa
    19561 agctgatgat gggtatattt taaatggtgt gaagacatat acatcaatga gtaaagcact
    19621 aacacatatt attgttgctg cttatataga agaattaaaa agtgttggtt ttttcttagt
    19681 ggacaggaat ttgcctggtg ttgaaattgc cgataattgg gacgtattag gtatgcgagc
    19741 gacagaaagt catgatttaa tattaaatga tgtaaaagta ccattaaaac atttagtgga
    19801 aacagagaaa agtaaagcgt caaatggttg gatattgcat ataccaagct gttatttagg
    19861 tatagcacaa gctgctagaa attatgctgt agactttgca atccaacata gccctaatag
    19921 tatagaagga acgattgcaa cattgccaac tgtgcaacaa aatttaggaa aaatggaaac
    19981 gctgttgtta tgtgcgagac aatttttatg gagtacagcg aaaggctatc aacaatatac
    20041 agccgaaagt cagattagaa atgcgacgag tgcgagtaaa gtaatggtaa tgaaccaagg
    20101 ccttgaagtg attgatttag cgatgcgcat tgttggtgct aaaagtttag aaatgaatcg
    20161 gccattacaa cgttactatc gtgacatgcg tgcaggatta cacaatccac ctatggaaga
    20221 tgcagcatat acaaatattg ctaagagtat tacagataca ttttaaattt aaaaaggtaa
    20281 atcgatcgtt gattggcata aagagatgaa atttgacgtt cggatttacc ttttttgcgg
    20341 gaatttatag gtatattaag atattttgct aaattttttg cgaaaataaa aaaataaata
    20401 acactagcta ttgtaaatgt atatgcaatc ttgtataatt ttggaataat tgataatcat
    20461 tttcaatagg aggaaattat gaaaaaacta ttattaccat taataattat gttattagtg
    20521 ttagctgcgt gtgggaacca aggtgaaaaa aataacaaag ctgaaactaa atcttataaa
    20581 atggacgatg gcaaaacggt agatattccg aaagacccta aacgcattgc agtagttgcg
    20641 ccaacatatg ctggtggact taaaaaatta ggtgcaaaca ttgtagctgt aaatcaacaa
    20701 gttgatcaaa gcaaagtgtt aaaagataaa tttaaaggtg ttactaaaat tggtgatggt
    20761 gatatagaaa aagttgctaa agaaaagcca gatttaatta ttgtatactc tactgacaaa
    20821 gacattaaaa agtatcaaaa agtagcacca acagtagttg ttgactataa taagcataaa
    20881 tacttagaac aacaagaaat gttagggaaa attgttggta aagaagataa agtaaaagct
    20941 tggaagaaag attgggaaga aacaactgct aaagacggta aagaaattaa aaaagcaatt
    21001 ggacaagatg caacagtgtc attgtttgat gaatttgata aaaaattata cacttacggc
    21061 gataactggg gtcgtggtgg agaagtatta tatcaagcat ttggtttaaa aatgcaacca
    21121 gaacaacaaa agttaactgc aaaagcaggt tgggctgaag tgaaacaaga agaaattgaa
    21181 aaatatgctg gtgattacat tgtgagtaca agtgaaggta aacctacacc aggatacgaa
    21241 tcaacaaaca tgtggaagaa tttgaaagct actaaagaag gacatattgt taaagttgat
    21301 gctggtacat actggtacaa cgatccttat acattagatt tcatgcgtaa agatttaaaa
    21361 gaaaaattaa ttaaagctgc aaaataattc agctatataa gccagtgaaa tgagagtctg
    21421 aaacatatca atcttttgat attgtattag gctcttattt ttatagcaag aaggttagat
    21481 ttttgtattt ttttaaataa taagtgccgt tattatcatt caatttaatt aatgatagat
    21541 tagtattatt acagctaaag tagtatacct gagaaaatag ctcaatgtat ctctgcatta
    21601 ataagttata tcataattat tttagtgcat actttatgga aggcatatca gggaatgcct
    21661 atcaattaaa gaagaggttt aaaaggatta caacagaatg ttatgatttt gtagaaagat
    21721 atattacaac gttttataaa aacataatat tgttaatgga aaatgaaatg taagggggat
    21781 ttcgagtgac taagaaagtt tattttaacc atgatggtgg tgtagatgat ttagtatctc
    21841 tatttttatt attacaaatg gaaaacgttc aattgatagg ggtcagtaca attggtgctg
    21901 attgttattt agaaccatct ttgagcgcat cagtaaaaat tattaatcgt ttttcaaagg
    21961 aagatattca agttgcgcca tcatatgaac gaggaaaaaa tccatttcct aaagaatggc
    22021 gtatgcatgc cttttttatg gacgcattgc caattttaaa tgagccagtc aaacacgttg
    22081 cttcaaatgt gagagacaaa gaagcctttg aagacattat tcaaacttta aagagacaat
    22141 cagaaaaagt aacattatta tttacaggcc cgcttacaga tttagcaaaa gcactacaaa
    22201 aagattcatc aatcgttcag tatatagaaa aattagtttg gatgggtggt acctttttac
    22261 caaaaggaaa tgttgaagaa cctgagcatg atggttctgc agaatggaat gcatattggg
    22321 atccagaagc ggttaaaatt gtttttgata gcgatataga gattgatatg gttgctttag
    22381 aaagta
//
LOCUS       DAMZPP010000029        22110 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMZPP010000029 DAMZPP010000000
VERSION     DAMZPP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22110
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.029"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            188..715
                     /gene="pyrR"
                     /locus_tag="POH73_000862"
     CDS             188..715
                     /gene="pyrR"
                     /locus_tag="POH73_000862"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDI6886834.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            933..2240
                     /locus_tag="POH73_000863"
     CDS             933..2240
                     /locus_tag="POH73_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDI6886835.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIFVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            2268..3149
                     /locus_tag="POH73_000864"
     CDS             2268..3149
                     /locus_tag="POH73_000864"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDI6886836.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            3167..4441
                     /locus_tag="POH73_000865"
     CDS             3167..4441
                     /locus_tag="POH73_000865"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDI6886837.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGIDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETVFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            4443..5543
                     /gene="carA"
                     /locus_tag="POH73_000866"
     CDS             4443..5543
                     /gene="carA"
                     /locus_tag="POH73_000866"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDI6886838.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGIKRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAILMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            5536..8709
                     /gene="carB"
                     /locus_tag="POH73_000867"
     CDS             5536..8709
                     /gene="carB"
                     /locus_tag="POH73_000867"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDI6886839.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHNFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQCLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            8819..9511
                     /gene="pyrF"
                     /locus_tag="POH73_000868"
     CDS             8819..9511
                     /gene="pyrF"
                     /locus_tag="POH73_000868"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDI6886840.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            9511..10122
                     /locus_tag="POH73_000869"
     CDS             9511..10122
                     /locus_tag="POH73_000869"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDI6886841.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKQAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            10152..10364
                     /locus_tag="POH73_000870"
     CDS             10152..10364
                     /locus_tag="POH73_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886842.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTINHNDDLFKHVKDILRKQGQI"
     gene            10801..11202
                     /locus_tag="POH73_000871"
     CDS             10801..11202
                     /locus_tag="POH73_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6886843.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(11465..13162)
                     /locus_tag="POH73_000872"
     CDS             complement(11465..13162)
                     /locus_tag="POH73_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDI6886844.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKMNPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVNRRQFMTS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLHKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYEHIQKMKQS"
     gene            13437..14060
                     /gene="gmk"
                     /locus_tag="POH73_000873"
     CDS             13437..14060
                     /gene="gmk"
                     /locus_tag="POH73_000873"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDI6886845.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLDHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            14060..14278
                     /locus_tag="POH73_000874"
     CDS             14060..14278
                     /locus_tag="POH73_000874"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDI6886846.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            14494..15693
                     /gene="coaBC"
                     /locus_tag="POH73_000875"
     CDS             14494..15693
                     /gene="coaBC"
                     /locus_tag="POH73_000875"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDI6886847.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            15693..18101
                     /gene="priA"
                     /locus_tag="POH73_000876"
     CDS             15693..18101
                     /gene="priA"
                     /locus_tag="POH73_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDI6886848.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIEVPSTLLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEERTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     EILQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMEASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
     gene            18604..19557
                     /locus_tag="POH73_000877"
     CDS             18604..19557
                     /locus_tag="POH73_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886849.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKFEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVMPALAYFTFSPKNYEDKSNPDPKVLN
                     LVGMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(19720..19923)
                     /locus_tag="POH73_000878"
     CDS             complement(19720..19923)
                     /locus_tag="POH73_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HDI6886850.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            20222..20710
                     /locus_tag="POH73_000879"
     CDS             20222..20710
                     /locus_tag="POH73_000879"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDI6886851.1"
                     /translation="MAIKKLVPASHPILTKKAQAVIKFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            20703..21638
                     /locus_tag="POH73_000880"
     CDS             20703..21638
                     /locus_tag="POH73_000880"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDI6886852.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPKLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            21635..>22110
                     /locus_tag="POH73_000881"
     CDS             21635..>22110
                     /locus_tag="POH73_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDI6886853.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNVMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATH"
BASE COUNT         7924 a         3208 c         4157 g         6821 t
ORIGIN      
        1 cagagagacg tcaaagacat gaaaatatag atatatacta aaatgaatca taacactttc
       61 acatatgcaa gaataagtag taatactacg attcttctat gtaaatagga tgattgtata
      121 tactaaacct catgtcgata ttaaagacat gaggtttttt gtttctaaat tttgaaaggg
      181 tgagaatatg tctgaacgta tcataatgga tgatgccgca atacaacgta cagtaacgag
      241 aatcgctcat gaaattttgg agtataacaa aggtactgat aatttaattc ttttaggtat
      301 caaaacaaga ggtgaatatt tagcgaatcg tatacaagat aaaattcatc aaattgagca
      361 acaacgtata cctactggaa caattgatat tacatacttt agagatgata tagagcacat
      421 gtcatcactt acgacaaaag acgcaataga catcgacaca gatattacag ataaagtagt
      481 catcattatt gacgatgtgc tgtatactgg tcgaacggtt cgtgcttcac ttgatgctat
      541 tttgctaaat gctagaccta ttaaaattgg tttggctgct ttggttgatc gaggacatcg
      601 tgagttacca attcgagcag attttgttgg taaaaatata cctacttcta aagaggaaac
      661 ggtaagtgtc tatttagaag aaatggatca aagaaatgca gttataatta aataataccc
      721 cttttaattc agtacgagag actgaaaaag gtgggggagt tgaatgataa ataagttttc
      781 atatgcttgt ttatctaata ataccctcat gttcgacaac tataagtcaa tttctatgga
      841 cgaaaatgac ttgtagcaac ttgtcacact ttttgtctct ttacatatac tgatgtaaag
      901 agattttttt attatcggag gaaatgaata ttatgcaaaa tgatgaaatg tttgaacgaa
      961 cagtaaaacc tgtactagat gtaaatgaaa aaccacaacc agcgcaatgg gcatttttaa
     1021 gcttacaaca tttatttgcg atgtttggcg caacagtact agtaccattc ttaacaggat
     1081 taccaatatc agcagcgtta ctagcttcgg gaatcggtac attactttat atcttaataa
     1141 cgaaggcgca aataccagca tacttgggat ctagctttgc atttatcaca ccaattatca
     1201 cgggattaag tacgcatagc ttaggagaca tgcttgtagc attattcatg agtggtgtta
     1261 tgtacgtcat catcgggatt ctaatcaaat taagtgggac agcatggtta atgaaattat
     1321 taccaccagt tgttgtagga ccagtcatca tggtaattgg tttaagctta gcgcctactg
     1381 cagtcaatat ggcaatgtat gaaaatcctg gagatatgaa aggttacaat atcagtttct
     1441 taattgttgc aatgataacc ttgcttgtaa caatattcgt tcaaggattt tttaaaggat
     1501 tcctatcttt aattccagta cttgtaggta ttatcgtagg ttatgtggta gcgattttta
     1561 tggggattgt gaaatttgat gcaattatgt cagcaaaatg gatagatttc cctcatattt
     1621 atctgccatt taaagattat gtaccttcat ttcacttagg acttgtactt gtaatgattc
     1681 cgattgtgtt cgtaacagta agtgaacata ttgggcacca aatggtactg aataaaatcg
     1741 taggtagaaa cttctttgaa aagccaggac ttgataaatc aatcattggt gatggtgttt
     1801 ctacaatgtt tgccagtatt attggtggac caccaagtac aacatatggt gaaaatatcg
     1861 gtgtattagc gattacaaga atatacagta tttacgtcat tggtggtgcg gcagttatag
     1921 caattgtttt agcattcatt ggtaagttca ctgcattaat ttcttctata cctacaccag
     1981 ttatgggagg agtatctata ttacttttcg gtattattgc agcaagtggc ttaagaatgt
     2041 tagttgaaag caaagtagat tttgcgaaca atcgaaattt agttatagct tcagtaattt
     2101 tagttgtagg tatcggtaat ttagtattta acttaaaaga aattggtatc aaccttcaaa
     2161 ttgaaggaat ggcattagct gcactttcag gaattatttt gaacttaatc ttacctaaag
     2221 agaaaaaaca aaacaattaa gatttacaaa ttaaggaggg cgcttttatg aatcatttat
     2281 tatcaatgga acatttatct acagatcaaa tatacaaact tatccaaaag gcaagtcaat
     2341 ttaaatctgg tgaacgtcaa ctaccaaact ttgaagggaa atatgtcgca aatttattct
     2401 ttgaaaattc tactcgaaca aaatgtagtt ttgaaatggc agaacttaag ctagggttaa
     2461 aaacgattag ctttgaaaca tcaacatcat ctgtttcaaa aggtgaatct ttatatgaca
     2521 catgtaaaac tttagaaagt attggctgtg atttattagt cattagacat ccgtttaata
     2581 actactatga aaaattagcg aatattaaca tcccaattgc gaatgctggt gatggtagtg
     2641 gacaacatcc aacacaaagt ttgcttgatt taatgacgat atatgaagaa tatggatatt
     2701 ttgaaggctt gaatgtattg atttgtggcg acattaaaaa ttcacgtgtc gcacgtagta
     2761 attaccatag tttaaaagca ttaggtgcaa acgtaatgtt taatagccca aatgcttgga
     2821 ttgatgattc tttagaagcg ccttatgtaa atatagatga tgttatagaa acagtagata
     2881 tagttatgtt attaagaatt caacatgaaa gacatgggct tgcagaagaa actagatttg
     2941 cagcagatga ttatcatcaa aagcatggct taaatgaagt gcgttataac aaattacaag
     3001 aacatgctat tgtcatgcat ccggcacctg tgaatagagg agtagaaata caaagcgatt
     3061 tagtagaagc ttcaaaatca agaattttta agcaaatgga aaatggcgtt tacttaagga
     3121 tggcagtcat tgatgaatta ttaaaatagg taaggggacg aaaatgatga aattaattaa
     3181 aaacggtaaa gtattacaaa atggtgaatt acaacaagca gatattttaa ttgatggtaa
     3241 ggtaattaaa caaattgcac ctgcaattga accaagcaat ggtattgaca tcatagatgc
     3301 gaaaggtcac tttgtgtcac ctggatttgt cgatgttcat gttcatttac gcgaacctgg
     3361 tggtgaatat aaagagacaa ttgaaactgg tactaaagct gctgctagag gcggatttac
     3421 aactgtatgt ccaatgccta acacaagacc ggtaccagat tctgtagaac attttgaagc
     3481 tttacaaaaa ttaatcgatg acaatgctca agtacgtgta ttaccttatg cttcaattac
     3541 aacacgtcaa ttaggtaaag aattggttga tttcccagca ctagtaaaag aaggtgcctt
     3601 tgcgtttaca gatgacggtg taggagtaca aactgcaagc atgatgtatg aaggcatgat
     3661 tgaagctgca aaagtaaaca aagccatcgt agcacactgt gaagataatt cattaatcta
     3721 tggtggtgca atgcatgaag ggaaacgcag taaagagtta ggtataccag gtattccaaa
     3781 catttgtgaa tctgttcaaa tcgcaagaga tgtactatta gctgaagcag caggttgtca
     3841 ttatcatgta tgtcatgttt ctactaaaga aagtgttaga gtcattcgtg atgctaaacg
     3901 cgcaggcatt catgttacag ctgaagttac accacaccat ttattgttaa cagaagatga
     3961 tattcctggt aataatgcca tttataaaat gaatccacca ttgagaagta ctgaagatag
     4021 agaggctttg ttagaagggt tactagacgg tacaattgac tgtatcgcaa cagaccatgc
     4081 accacatgca cgtgatgaaa aagcacaacc aatggaaaaa gcaccattcg gaattgttgg
     4141 cagtgaaaca gtattcccat tattatatac gcattttgta aaaaatggtg attggacatt
     4201 acaacaatta gtagattact taacaattaa accatgtgaa acatttaatt tagaatacgg
     4261 cacattaaaa gaaaatggtt atgcagattt aacaatcatt gatttagata gtgaacaaga
     4321 aattaaagga gaagatttct tatcaaaagc agataataca ccatttatcg gctataaagt
     4381 ttatggaaat ccgatcttaa caatggttga aggcgaagtt aaatttgagg gggataaata
     4441 atatgcaaag caaacgttat ctagtgttag aagacggttc tttttacgaa ggctaccgtt
     4501 taggatctga taacttaact gtaggagaaa ttgtatttaa tacagcgatg acaggttatc
     4561 aagaaactat ttcagatcca tcatatacag gtcagatcat tacttttacg tatccattaa
     4621 tcggtaatta tggtatcaag agagacgatt ttgaatcatt agtacctaca ttaaacggta
     4681 ttgtagtgaa agaagcgagt gcgcatccaa gtaattttag acagcaaaag acacttcatg
     4741 acgttttaga attgcatcaa attccaggga ttgcaggtgt tgatacaaga agtattacgc
     4801 gtaaaattcg acaacacggt gtgttaaaag ctggttttac tgatcgaaaa gaagatattg
     4861 atcaacttgt caaacattta caacaagtag aattacctaa aaacgaagta gaaatcgttt
     4921 cgactaaaac accgtatgtt tcgacaggta aggatctaag tgtcgtactt gtagactttg
     4981 gtaagaagca aaatattgtt cgagaattaa acgtcagagg ttgtaacgtc acagttgtac
     5041 catatacaac tactgccgaa gaaattttag caatggctcc agatggcgtt atgctatcaa
     5101 acggaccagg taatcctgaa gttgtagaat gtgcgattct aatgattcaa ggaattttag
     5161 ggaaaattcc gttctttggt atctgtctag gacatcaact ttttgcatta tctcaaggag
     5221 caagctcatt taaaatgaag tttggtcatc gtggtgcgaa ccatccagtt aaaaatttag
     5281 agactggaaa agttgatatt acgagtcaaa accatggata tgcaatagat atagattcgt
     5341 taaaaagtac tgatttagaa gttactcatc ttgcattaaa tgatggtact gtagaaggtt
     5401 taaaacataa aacattacca gcattttctg ttcaatacca tcctgaagca aatccaggac
     5461 cgtcagattc aaactatcta tttgatgatt ttgtagcaat gatgactaat tttaaggaaa
     5521 aggagcgtca tatcaatgcc taaacgtaat gatatcaaaa caattttagt aatagggtct
     5581 gggccaatta tcataggtca agcagctgaa tttgattatg ctggaacaca agcatgtcta
     5641 gctttaaaag aagagggata ccgagttatt cttgtaaatt caaatccagc gacaatcatg
     5701 actgataagg aaattgcgga taaagtatat atcgaaccgt taactcataa ttttatagcg
     5761 cgaattatac gtaaagagca acctgacgct ttacttccaa ctttaggtgg tcaaacaggt
     5821 ttaaacatgg cgattcaact acacgaaagt ggtgtgcttc aagataataa cgtccaatta
     5881 ttaggaactg agctaacatc aattcaacaa gcagaagacc gtgaaatgtt tagaacatta
     5941 atgaatgatt taaacgttcc tgtgccagag agtgacattg taaatacagt agagcaagcc
     6001 tttaaattca aagagcaagt gggatacccg ctaattgtta gaccagcatt tacgatgggt
     6061 ggtaccggag gcggtatttg tcataatgat gaagaattac atgaaatcgt ttcaaatggt
     6121 cttcattata gtccagcaac gcaatgttta ttagaaaaat ctatcgcagg ttttaaagaa
     6181 atcgaatacg aagtaatgcg tgataaaaac gataatgcca tcgttgtatg taacatggaa
     6241 aatattgatc cagttggtat tcatacaggc gattcaattg ttgtagctcc tagccaaaca
     6301 ttatcggatg ttgagtatca aatgttacgt gatgtttcat taaaagttat tcgagcatta
     6361 ggtatcgaag gtggttgtaa tgttcaatta gcattagatc cccattcatt cgattattat
     6421 attatagaag taaatccgcg tgtatcacgt tcatcagcgt tagcttcaaa agcaacagga
     6481 tatcctattg caaaattagc tgctaaaatc gcggttggtc taacattaga tgaaatgtta
     6541 aatccaatta caggaacatc ttatgcagcg tttgaaccaa ctttagacta tgtgatttca
     6601 aaaataccaa gatttccttt tgataaattt gaaaaaggag aacgagagct tggcacacaa
     6661 atgaaagcaa caggtgaagt tatggccatt ggtcgaactt acgaagaatc attgttaaaa
     6721 gcaattcgat cacttgagta tggtgtgcat cacttaggat taccaaatgg tgaaagcttc
     6781 gatcttgatt atattaaaga acgtatttca caccaagatg atgaacgatt atttttcatc
     6841 ggcgaagcaa ttagaagagg cacaacatta gaagaaattc ataatatgac tcagatagat
     6901 tacttcttct tacacaagtt ccaaaacatt attgatattg agcatcaatt aaaagagcat
     6961 caaggtgatt tagaatatct taaatatgca aaagattatg gatttagtga taaaacaata
     7021 gcgcatcgct ttaatatgac ggaagaagaa gtatatcaat tgcgtatgga aaatgatatt
     7081 aaacctgttt acaagatggt tgatacttgc gcagctgaat ttgaatcttc aacaccatat
     7141 tattatggta catacgaaac tgaaaatgaa tccatagtta ctgacaaaga aaaaatctta
     7201 gtattaggct ctggaccaat tcgaatcggc caaggtgtag aatttgacta tgcgacagtt
     7261 cacgccgttt gggcaattca aaaagcaggg tacgaagcga taattgtgaa taacaatcca
     7321 gaaacagttt caacagactt ctcaatttct gacaaattat actttgaacc tttaactgaa
     7381 gaagatgtga tgaatatcat taatttagaa aaacctaaag gtgtcgttgt acaatttgga
     7441 ggacaaacag cgattaattt agcagacaaa ttggctaaac atggtgttaa aatacttggt
     7501 acttcactag aaaatctaaa tcgtgctgaa gatagaaaag aatttgaagc actattaaga
     7561 aaaattaacg tgccacagcc acaagggaaa acagctacat cacctgagga agcattagcg
     7621 aatgctgcag aaatcggata tccggttgta gtaagacctt cttatgtatt aggtggtcgc
     7681 gcaatggaaa ttgtagacaa tgacaaagag ttagaaaact atatgaccca agctgtaaaa
     7741 gcgagtccgg aacatccggt attagtcgat agatatttaa ctggtaaaga aattgaagtt
     7801 gatgcgattt gtgatggaga aacggtcatt attccaggaa ttatggaaca tattgaacgt
     7861 gctggtgtgc atagtggtga ctcaatcgct gtatatccgc cacaaacttt gacagaagac
     7921 gagttagcaa cgcttgagga ctatactata aaattagcta aaggtttaaa catcattggc
     7981 ttaatcaaca ttcaattcgt tatagctcac gatggtgtgt atgttttaga agtaaatcca
     8041 cgttctagta gaacggtacc attcttaagt aaaattactg atattccaat ggcacaatta
     8101 gctatgcgag caatcattgg ggaaaaacta acagatatgg gttatcaaga aggggttcaa
     8161 ccatatgctg agggtgtatt tgtgaaagca ccggtattta gttttaataa attgaaaaat
     8221 gttgatatta ctttaggacc tgaaatgaag tctacaggtg aagtaatggg gaaagataca
     8281 acattagaaa aggcgttatt caaagggtta acaggtagtg gtgttgaagt taaagatcac
     8341 ggtacagtat taatgactgt cagtgacaaa gataaagagg aagttgttaa attggcacaa
     8401 tgcttaaatg aagttggcta taaaatttta gcaacgtctg gaacagctaa taaattagct
     8461 gagtatgaca tacctgcaga agtagtaggc aaaattggtg gcgaaaatga tttattaaca
     8521 cgtattcaaa atggtgatgt tcaaatcgtt ataaatacaa tgactaaagg taaagaagta
     8581 gaaagagatg gcttccaaat tagacgtact acagttgaaa atggtattcc atgtttgaca
     8641 tctttagata cagctaatgc cttaacgaat gtaattgaaa gtatgacatt tacaatgcgt
     8701 caaatgtaaa tcaatcaaac tgtatcggtg gggctgtaat taaccattta cttaaagaag
     8761 tttatattac agcctcatta ttttagtgaa tttcttaacg taaagggaga catatatgat
     8821 gaaagattta ccaattattg cattagattt tgaatcaaaa gaaaaagtaa atcaattttt
     8881 agatttattt gatgaatcat tattcgtaaa agtaggtatg gaactttttt atcaagaagg
     8941 tcctcaatta attaatgaga taaaagaaag aggccatgat gtatttttag atttaaaact
     9001 gcatgatatt cccaatacag ttggtaaagc gatggaagga ctagctaaat tgaatgttga
     9061 tctggtaaat gttcatgctg ctggtggcgt aaaaatgatg tctgaggcca ttaaaggatt
     9121 aagaaaacat aatcaacata caaaaattat tgcagtaaca cagcttacgt caacaacaga
     9181 agacatgtta cgacacgaac aaaatataca aacatcgatt gaagaggccg ttttaaatta
     9241 tgccaagtta gcaaatgcag ctggtttaga tggcgttgtt tgttcacctc ttgaaagtcg
     9301 tatgttgact gaaaagttag gtacatcatt tttaaaagta acaccaggca ttagacctaa
     9361 aggtgcatct caagatgacc aacaccgtat tacgacaccg gaagaagcaa gacagcttgg
     9421 ttcgacgcat attgtagtcg gtagaccgat tacacaaagt gacaatccag tcgaaagtta
     9481 tcataaaatt aaagaaagtt ggttagtata atggctaaag aaattgcaaa atcattatta
     9541 gatattgaag ctgtaacatt atcaccaaat gatttatata catggagttc aggtattaaa
     9601 tcaccgattt actgtgataa ccgtgttacg ttaggttatc ctttagttcg aggcgcaatc
     9661 cgcgatggtt taattaactt aattaaagaa cactttcctg aagtagaagt tatttctggt
     9721 actgcaacag ctggtattcc acatgcagct tttattgctg aaaaattaaa attaccaatg
     9781 aattatgttc gttcatcaaa taagagtcat ggtaagcaaa atcaaattga aggtgctaaa
     9841 agtgaaggta aaaaagtagt tgtgatagaa gatttaattt cgacaggggg atcttcagtc
     9901 acagcagttg aagccttaaa acaagcaggt gcagaagtat taggtgttgt tgctatcttt
     9961 acttacggtt tgaaaaaagc agatgataca tttagcaata ttcaactacc tttttacact
    10021 ttaagtgatt acaatgaatt aattgaagta gctgaaaatg aaggtaaaat ttctagtgaa
    10081 gatatccaaa cattagttga atggagagac aacttagcat aatatagaca ctagaaggag
    10141 gaattcaaca aatgaatgac aaaacatcta atgatttata tgggaagata aaacattgta
    10201 acgaatttat caatcattca aatgattcca atctatctag tagtcacgat gtcgacgaaa
    10261 gttcaacgaa gcaaaaacat ataaaaaata aaacaactat aaatcataat gatgatttat
    10321 ttaaacatgt aaaggatata ttacgtaaac aaggacaaat ttaaaaacaa aaaagctata
    10381 gtgtttaata tcaggggctg cttaaccgca tccgaatatt acaaacacta tagcttttat
    10441 tatataaaaa atttattagc ggataattaa ctttattaaa atccaaccaa ttaagaatac
    10501 gagtaatagg actaatactg gaatcacata atgtaacata acgtccctcc tttaacttaa
    10561 ttttaattgt aatcaaattt gacaataagt caaaacatta atacctatga taagtatcat
    10621 ttattaacat atgtatcata tttttaatct tgcgtaattt ttatcgttaa ctatgtgatt
    10681 taatcaacag gccaataaga catttgtcat tcgtgcacat aacggtacga cgcgcttttg
    10741 tcattttaat tatgttaaga taatagtagt tatagaagtt caatctatag gaggcatagc
    10801 atggatattc caaaaattac gacattttta atgtttaata accaagctga agaagctgtt
    10861 aaactataca caagcttatt tgaagatagt gagattataa caatggctaa gtatggtgaa
    10921 aatggacctg gtgatcctgg gactgtacaa cactcaatat ttacattaaa tggacaagta
    10981 ttcatggcga ttgatgctaa tagtggcaca gaattaccaa tgaatcctgc gatttcatta
    11041 tttgttacag taaaagatac tattgaaatg gaacgactat ttaatggatt aaaagatgaa
    11101 ggtgccattt taatgccaaa aacgaatatg ccaccataca gagagtttgc ttgggttcaa
    11161 gataagtttg gagtaagttt tcaattagca ttacctgagt aaaaggattc gtacagcgtt
    11221 gaatgataaa gaaacacttt ttcttatgca tgcgttttac ctatgtattc ctattttaag
    11281 tacacattat cattttagtt tcactcattt ttaaaatcac aaagataatt tttaacttaa
    11341 atgatgatac gttattaaaa aacacgatac ttcgtttcaa tgaacgcatc aaattaaaaa
    11401 taaacacctc gcaaaagaaa gtaggccttt cttatggtga ggtgtttatt tgtcgacaac
    11461 ttgattatga ttgtttcatt ttttgaatat gttcataatc aggtgtagca tacaaagttt
    11521 tttgattgtc atatgttaca aacccaggct ttgcacctga tggtttgtgc acatttttaa
    11581 ttaacgtgta atcaacaggt atttgtccag aattaccagc ttttgaaaag tatcctgcta
    11641 acatagccgc ttccttgatt gtcgtatcac ttggtgcatc attaaatata acgacatgtg
    11701 aaccaggaat atcttttgtg tgtaaccatg tgtgagtttt tttagctttt ttatttgtta
    11761 aataatcatt ttgcttgtta ttcttgccaa catatatatc gtcgccatca gttgatacat
    11821 aatgttgtaa ttgaatctgc gctttctttt tcttagtttg atttttacgc tgtttcataa
    11881 agccttgttc tgctaattca tctctaattt catcaatgtc atggacagaa atatgatgta
    11941 attgttgttc gattgttgaa aaataatcta tattgtcttt cgtcaattga atttgatgtt
    12001 gtaattcacg ttctctcgtt ttcatacgat tatattgttt ataataatat tgagcatttg
    12061 cagatgggga ttttgtagga tttaaaggaa tgacaacttc ttcattcgta taataattca
    12121 atgccgtcac ttctttatcg ccttgcttaa ttcgatatat attagcagta atcaattcac
    12181 catataactg ttcagtatct ttatttttag actgctcata ttcttcaatc aacttcgcta
    12241 atttattttg atatttgtgc aactgctgtt gaacaaatcg aactaaatca ttcgcacgtt
    12301 gtttaacgcg ttcccgttcg ccacgcgcat cataaaaacg atcaagtaaa tcatttaatg
    12361 aatcgtacgt aactgtatca tcattaaatt gatttaactt tataaaatag aaatcctctt
    12421 tacctgtttc atgattttta tgaaaaatag gagtaggtgg taacttggtt tctgccatta
    12481 cttcgtcgaa tgcttctggt aaagttgatg aagtcataaa ttgacgacga ttaacgattt
    12541 cattcgtaat taaagggcta aacccttcaa actgattcaa taattgttta gcaatattac
    12601 ctgcgttaaa atcgatatat ttcaacacct ctgcacctgt aatatcatac ggattcattt
    12661 tgtgctgagt aggtggtgct tcataattaa atcctggcat tactgttcga tagtgattcg
    12721 tatttggtgt taagtgttta aatccttcaa ttattttacg attttcatct actaaaatta
    12781 agttactatg tttacccata atctcaagga tgacagtgcg gtaaatagta tcgccaattt
    12841 catctttact ctttatatcg atttcaatgc gacgatcatt accaatttgc ttaatcgatt
    12901 cgataatacc accttctaag tgttttctaa aaacacgcgc aaacatgggt ggattaaatg
    12961 gattatcata ttttttagta gtcaattgta atcttgaaaa gtttggatgg attgacaata
    13021 acaattgatg gttttgtcta ttttgacgta caaccattag tatcgtgtca ttatcaggtt
    13081 gattgatttt gtgaacgcgt cctgttgtta aaaattgtag agactcaacc attttctttg
    13141 taaataagcc atcataagcc ataatttatc ttccaccttc ttcatatcat tcgtttcatt
    13201 ttaacatgat tgtaactttt cggtatagtt attgaatcat acattatgcc acacatttaa
    13261 cattatctaa aagttcgcaa ttcaatttat ttaattaaaa ttaatcgtgc aatataacga
    13321 tattgttaga cctaatagaa attatggcat gcaatttcaa atatgctata caatataaag
    13381 aacaatgtga tatcatattt aaataataga agattagctt agagaggtcg taaggcatgg
    13441 ataatgaaaa aggattgtta atcgttttat caggaccatc tggagtaggt aaaggtactg
    13501 ttagaaaacg aatatttgaa gatccaagta catcatataa gtattctatt tcaatgacaa
    13561 cacgtcaaat gcgtgaaggt gaagttgatg gcgtagatta cttttttaaa actagggatg
    13621 cgtttgaagc tttaattaaa gatgaccaat ttatagaata tgctgaatat gtaggcaact
    13681 attatggtac accagttcaa tatgttaaag atacaatgga cgaaggtcat gatgtatttt
    13741 tagaaattga agtagaaggt gcaaagcaag ttagaaagaa atttccagat gcgttattta
    13801 ttttcttagc acctccaagt ttagatcact tgagagagcg attagtaggt agaggaacag
    13861 aatctgatga gaaaatacaa agtcgtatta acgaagcacg taaagaagtc gaaatgatga
    13921 atttatacga ttacgttgta gttaatgatg aagtagaact tgcgaagaat agaattcaat
    13981 gtattgtaga agctgagcac ttaaaaagag agcgcgtaga agctaagtat agaaaaatga
    14041 ttttggaggc taaaaaataa tgttaaatcc accattaaac caattaacgt cacaaattaa
    14101 atcaaagtat ttaattgcaa caactgcagc gaaaagagcg cgtgaaattg atgaacaacc
    14161 tgaaactgaa ttattaagtg aatatcattc atttaaacca gttggtagag cgttagaaga
    14221 aattgctgac ggtaaaattc gccctgttat ttcaagcgat tattatggta aagaatagtt
    14281 tcgaccatta aaaatatgtg aagcaagacg tatatcaaat acgtcaaggc atatccaaat
    14341 caaacgcacc tatgtaggtg tgttttttta gtttatccaa tgattgatgt tataataata
    14401 ctaaatttgt atctataaaa aagtaatgag catttgtgcg catatgatga tgtaaagcgt
    14461 aaatgtcata gtgatattta agggagattg aatatgaaga aaatattgtt agccgttaca
    14521 ggtggcattg cggcatataa agcgattgat ttgacaagta agttaacaca atctgggtat
    14581 gaagttcgcg ttatgttaac gaatcacgca caaaaatttg tgacaccatt agcatttcaa
    14641 gcaataagtc gaaatgctgt ttatacagat acttttatag aagaaaatcc ttcagaaata
    14701 cagcatatag cattaggtga ttgggcagat gcaatcattg ttgcacctgc aacggcaaat
    14761 acaattgcaa aattgagtgt aggtattgct gatgatttgg tgacatcaac gttgctagca
    14821 acagagacac cgaaatttat tgcgcctgct atgaatgtgc atatgtatga aaataaacgt
    14881 acgcagcaaa atattaatat tttaaaagaa gatgggtatc attttatcga accaggaagc
    14941 ggatttttag catgtggtta tgttgctaaa ggacgtatgg aagaaccgct tcaaatcgtt
    15001 tctgttattg atgctcattt tcaaaatagt aatcgtttag ctaatagttc atttcaagat
    15061 aaacgcgcat tggttacagc cggaccaact attgaagtta tcgatccagt cagatttgta
    15121 tccaatcgtt cttctggaaa aatgggttat gcaatagctg aagcattgcg aaatcgagga
    15181 gctatcgtga cattagttgc ggggcctaca acactagagg atccaaaaga tattgaagtt
    15241 attcatgttc aaagtgctga agaaatgttt gaacaagtga caagccgatt tgacgaacaa
    15301 gatattgttg taaaagcagc agccgtatct gactatacac cggttgatgt attagaacat
    15361 aagatgaaaa agcaggacgg tgatttgtca gtatctttta aacgtactaa agacattctt
    15421 aaatatttag gtgaacataa aacatcacag tatttaatag gctttgcagc agagactgaa
    15481 gatattgaaa attatgcaca acaaaaatta cgcaagaaaa atgcagatgt gattatttca
    15541 aataatgttg gagatatgtc tatcggattt agttctgatg ataatgaatt gacaatgcat
    15601 tttaaaaata atgaaaaagt aaatatcaag aaaggaaaaa aagtagtatt agctgcacaa
    15661 attttagatg aactagaaac taggtggcaa taatgatagc gaaagtcata gtcgatgtcg
    15721 cgtcgaagag cgttgactat aaatttgatt atataattcc cgaacaactc gaatctgtca
    15781 tccaacctgg tgtgcgtgtg attgtacctt ttggaccaag aacgattcaa ggttatgtaa
    15841 tggaagtaac agcagaacct gatgcacaac ttgacgtttc gaagttaaaa aaaatcatag
    15901 aagtgaaaga tatacaacca gaattaacat cagaattaat agctttaagt gagtggatgg
    15961 gttcaactca tgtcattaaa cgtatttcta tgctagaagt gatgcttccg agtgctatta
    16021 aagcgaagta taaaaaagca tttaagatga aagatgacat agaggtacct tcaactttat
    16081 tacaaaaatt tgataagcat ggttactatt attataaaga tgcgcaaaaa aataatgata
    16141 ttcaattgct tatgaagttg ttaaaagatg atatcgttga agaaagaacg attctcacac
    16201 aaaatataac taaaaaaacc aagcgtgctg ttcgtgtcat tgaagggtat catcctgatg
    16261 aagtattagc taagttggag aaagttatta aacaatatga tttgtatgct tacttgtctg
    16321 aagaacaaca taaaacaata tttttaactg atattgagga tatgggcttt tcaaaatcta
    16381 gtttagatgg acttatcaaa aaaggttatg ttgaaaaata tgacgcggtt gttgaaagag
    16441 acccatttaa agatcgtgtt ttcgaacaag aatcaaaaca gcaattaaca gaagaccaat
    16501 ataaagcata tgaagcgatt aaagctaaaa ttgtaagcca agagcaagaa acatttttac
    16561 ttcatggtgt gacgggatca ggtaaaacag aagtatattt acaaacgata gaagatgttt
    16621 taagtcaagg aaaacaggcg atgatgttag ttcctgaaat cgctctaaca ccgcaaatgg
    16681 ttttacgctt caaacgtcga tttggtgatg acgttgctgt attacattct ggcttatcta
    16741 atggggaacg ttatgatgag tggcaaaaaa ttagggatgg tcgtgcgaga gtaagtgttg
    16801 gtgcaaggtc aagtgtgttc gcacctttca aaaatttagg gttaatcatc attgatgaag
    16861 aacatgaatc tacatataag caagaagatt atccgagata tcacgctaga gaaattgccc
    16921 aatggcgaag tgaatatcat cactgcccag tcattttagg aagtgcaaca ccatgtcttg
    16981 aaagttatgc acgagctgaa aaaggtgttt atcatttgct atcattacca aacagagtga
    17041 accaacaagc tttacctgaa attgatatcg tagacatgcg tgaagaattg agtgaaggta
    17101 atcggtcaat gttttcaaaa gatttacgtg aagccataca attaagatta gatcgacagg
    17161 aacaagttgt tttattttta aatcgacgtg gttatgcatc atttatgtta tgtcgggatt
    17221 gtggatatgt accgcaatgc ccaaactgtg atatttcatt aacgtatcat aaaacgacag
    17281 acttattaaa atgtcactat tgtggttacc aagagacacc accgaatcaa tgtccaaatt
    17341 gtgagagtga acacattcga caagtaggta ctggtactca gaaagttgaa gaaatattgc
    17401 aacaagaatt tgaagatgcg cgcataatta ggatggatgt agatacaacc tcaaagaaag
    17461 gtgcacatga aaagttattg actgaattcg aaaaaggtaa cggtgacatt ttactaggta
    17521 ctcagatgat tgcgaaagga ttagattatc caaacattac tttagttggt gtgctgaatg
    17581 cagatacaat gttaaattta cctgattttc gggcgagcga acgtacttat caactattaa
    17641 cgcaagtggc tggtagagct ggtcgtcatg aaaaggcagg tcaagtcatc attcaaacgt
    17701 ataatccaga tcattattca atattggatg ttcaaaaaaa tgattattta acattttatc
    17761 gtcaggaaat ggaatatcgt aaattaggaa agtatccacc gtattattat ttgattaatt
    17821 tcacaatctc acataaagaa atgaagaagg ttatggaagc atcgcaacat gttcataaaa
    17881 ttttattaca gcatttaaca gaaaaagcgc ttgtactagg tccatctccg gcagcacttg
    17941 cgagaatcaa caatgaattt agattccaaa ttttagtgaa atataaaagt gaacctggat
    18001 tattacaagc cattcagttt ttagatgact attaccatga aaaatttata aaagaaaaat
    18061 tagcgttgaa gattgatatt gatccacaga tgatgatgta attttttgac taaaacactt
    18121 taataccaat gtttcctaag agtttgaaag gtcttgtttt tactttaagg gcagaaaaag
    18181 ggcagaatat acaactgtta accatttaaa ttagcagttt atattctgtc tttttatatg
    18241 gctttataac ttacgtgatt ttgatttgat tagggattta ttagtatttt catttacgaa
    18301 ttctgatttt aatgtaagtg ccatacttac tgataaataa aagtactaat gattatgcaa
    18361 actattttta aatggcaaat tgaaatggtg caaattttac acttgtaatt taatggctat
    18421 aaactatttt atatctatct atgttttaac tacttaaatt attagacaaa acaattttct
    18481 taaattatca attaaagtat ttaaaaatat tgatggttaa tgtaaaatat caatataaat
    18541 aagtttttta aatttatgta tgtttatttg attcaaacaa aataacttaa gaggagaaag
    18601 tttatgaaaa agacactggg atgtttactt ttaattatgc ttttagtcgt agcaggttgt
    18661 tcttttggtg ggaatcataa attatcatca aagaaatcag aagaatcaaa acaagaaact
    18721 gtaaaaaaag aatcggaaga agagaaagat ccagatttag agaaatttga agaaatagag
    18781 aagaaaatga aaggaattaa agatgcgcca tctctagata agttggatcc attaatgaca
    18841 gaaaagtcgt ttacgaatag taaagggatt caaggatgga aagattacaa agaattaatg
    18901 ggtaaagtgg aacttgcaga ttatagattt acaaaagatt caaaaggatc ttctataaaa
    18961 gatgttgatg cattctttaa aggtaagaaa ggtataaaaa ggaaagtgat tgaaacacac
    19021 gatgatgtaa aacaagttga ttattggtat gtagatccag atggaaagaa aattggcaat
    19081 tcaaacacac ctgtttttta cgcagaaatt atgacaaaat ataaagatgg aaagttagtt
    19141 tatgcatcag tcgaaccagg ttcttacgta atacataaag atgatgcaat taaatatgac
    19201 gattattcta agttaaaaaa attaagtcaa ctaactaaac ttgatcatcc aaagccagtt
    19261 ccatatagcg tagctcaaat caaatctttc ggagtacctt taacaagcgt ttcatttatg
    19321 acgcatggat caaaggatac taaagatgaa gtgatgccgg cattggcata tttcactttt
    19381 tcaccaaaaa attatgaaga caagtctaat ccagatccaa aagttttaaa tttagtaggg
    19441 atggatttct taaatgcatc tagtgatttt ggtaacgcac attttgttgt tctaagtaaa
    19501 tatattaaag agtacgaatc aaactatgaa acagcgtcag atgattcttt aaaatagtat
    19561 ttactgtgcg gaaaataaat agtgtgctac attaaataat cgcaataata atcccgataa
    19621 acaatcagca ttgctgctta tcatatagag ttcgtaataa ctataactct atgattcgca
    19681 aataataaat gattgtcatc gggatttatt tttatcaatt tataaagtga cattaccttg
    19741 ttcatcagca ggtttgaaaa cagtaatcac tgcactaata attgctaaaa tgtgtgggat
    19801 acctgtccaa cagaatatta agtgtagaat accttgcata ttcttgccgg cataaaattt
    19861 atgaatacca aaactaccta agaacaatgc taataaaata taaataactt tgtttacttg
    19921 catttctttc cctccagttg aattgcttat aatgacatta gcttctcttt ttattatacc
    19981 cacttttagt tcaaacattc tagtttcagc attcccaatc atctaaattt cattttttca
    20041 atccttacaa taaatttagg atttcatttc agttgcattg tattatttta cgtgtgaaat
    20101 atacgtaatg aatcacatta caatctttaa attgaaaaat atacattcta tgatgtaagg
    20161 tcgcattttt aatatattta cgttataatg gtttgatgaa ttttattgag gagtaagagc
    20221 tatggcgatt aaaaagttag taccagcatc gcatcctatt ttaacgaaaa aagcgcaagc
    20281 agttataaaa tttgatgatt cgttaaaaag attattacaa gatttagaag atacaatgta
    20341 tgcacaagaa gctgctggct tatgtgcacc tcaaattaat cagtcattgc aagtggcaat
    20401 cattgatatg gaaatggaag gattattaca acttgttaat ccgaaaatta ttagtcaatc
    20461 aaatgaaacg ataacagact tagaaggttc aattacattg ccagatgttt acggcgaagt
    20521 gacaagaagt aaaatgatcg ttgtcgaaag ttatgacgtc aatgggaaca aagttgaact
    20581 aactgcacat gaagatgtag caagaatgat tttgcatatt atagatcaaa tgaacggtat
    20641 cccttttaca gaacgtgcgg accgtatttt aacagataaa gaagtggagg catattttat
    20701 aaatgactaa aataatattt atgggtacac cagacttttc aacaactgtt ttagaaatgc
    20761 ttattgcaga acatgatgtc attgcagtcg taacgcaacc agatcgacct gttggacgta
    20821 aacgtgttat gacaccacca ccagttaaaa aagttgcaat gaaatatgat ttacctgtat
    20881 atcagcctga aaaattaagt ggatcagaag aattagaaca attgcttcaa ttagatgtag
    20941 atttaattgt aacagccgct tttggacaat tattacctga atcattgttg gcattaccaa
    21001 aacttggcgc aattaatgta catgcatcat tgttgccgaa gtatagaggt ggtgcaccaa
    21061 ttcatcaggc aattatcgat ggtgaacaag aaaccggcat aacaattatg tatatggtta
    21121 aaaaattaga tgcgggtaat attatttcgc aacaagcaat taaaatagaa gaaaatgata
    21181 atgtcggtac gatgcatgat aaattaagtg tattaggagc agatttatta aaagaaactt
    21241 taccatcaat tatagagggt acaaatgaaa gtgtaccaca agatgatacg caagcaacat
    21301 ttgcttccaa tattcgacgc gaagatgagc gaattagctg gaataaacca ggaagacaag
    21361 tgtttaatca aattcgagga ttatcaccat ggccagttgc ttatacaact atggatgaca
    21421 ctaacttgaa aatatacgat gctgaactcg ttgagactaa taagataaac gagcctggaa
    21481 caattataga aacgactaaa aaagccatta ttgttgctac aaatgataat gaagctgttg
    21541 caattaaaga tatgcaattg gctgggaaaa agagaatgtt agctgccaat tatttaagtg
    21601 gtgcgcaaaa cacactagta gggaagaaac ttatatgata gaaaacgtga gaagtcttgc
    21661 ttttgacacg attcaagata tattaaatga aggtgcgtat agtaacttgc gtatcaatga
    21721 agtgttgtca gaaaatgaat taaatgtaat ggataaagct ttatttacag aaattgtcta
    21781 cggaaccgtt aaaagaaaat atacgttaga tttttattta aagccttttg tgaaaacaaa
    21841 gattaaggca tgggttaggc aattattatg gatgagtatt tatcaatatg tttatttaga
    21901 taaagttcca aatcacgcca tcattaatga agcagttgaa atagcaaaag aacgcggtgg
    21961 ctatcacaac ggaaatgtcg taaatggcat tttacgaaca atgatgcgta gtgacttacc
    22021 tgattttaat gaaattgcag atcctaaaaa aagaatggca atcgagtata gtatgccgaa
    22081 gtggattata gatcattggg caacacatta
//
LOCUS       DAMZPP010000030        22067 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMZPP010000030 DAMZPP010000000
VERSION     DAMZPP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22067
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.030"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(33..890)
                     /locus_tag="POH73_000882"
     CDS             complement(33..890)
                     /locus_tag="POH73_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDI6886854.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINRLREKGYKVFLAT
                     GRSHSEIHQLVPEDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQIINLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     EHFGINQKETLVIGDSDNDRAMFEFGHYTVAMKNVRPEIQALTSDVTAYTNEEDGAAK
                     YLAKHFLAD"
     gene            complement(958..1740)
                     /locus_tag="POH73_000883"
     CDS             complement(958..1740)
                     /locus_tag="POH73_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6886855.1"
                     /translation="MLIQLDQVGRMKQGKTILKNISWQINEGDKWILYGLNGAGKTTL
                     LNILNAYEPATTGYVNLFGKMPGKVGYSTETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEVRNEAHQLLKLVGMSAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPTLAMIYVTHFIEEITGNFTKILLLK
                     DGESVQQGLIDDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            complement(1972..3777)
                     /gene="glmS"
                     /locus_tag="POH73_000884"
     CDS             complement(1972..3777)
                     /gene="glmS"
                     /locus_tag="POH73_000884"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDI6886856.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVTLQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            4458..5996
                     /locus_tag="POH73_000885"
     CDS             4458..5996
                     /locus_tag="POH73_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDI6886857.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWFPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFFGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLATLVSFV
                     VAALIMKFTKEPKQDLEAATAQMETTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SLDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            6031..8163
                     /locus_tag="POH73_000886"
     CDS             6031..8163
                     /locus_tag="POH73_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDI6886858.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRITGADSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNLAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVKKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLEQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETRDKSSNMI
                     DKDDVHIETKDIDGNTSFENEQTSYLTSVFEKHLSDEKSEQLMHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFIPDNDFMASLISDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            8175..8609
                     /locus_tag="POH73_000887"
     CDS             8175..8609
                     /locus_tag="POH73_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6886859.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEGNVDRIVQAKSAEEIKQVFEEADA"
     gene            8609..9715
                     /locus_tag="POH73_000888"
     CDS             8609..9715
                     /locus_tag="POH73_000888"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDI6886860.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALATGH
                     QYDVILADESKTTTRVNNVAAINSMQPSEELKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVKDSSIEAGLRRVLAETSQYITNEFDFTEAEQAGYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLAYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(10034..17422)
                     /locus_tag="POH73_000889"
                     /pseudo
     CDS             complement(10034..17422)
                     /locus_tag="POH73_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(17705..19060)
                     /locus_tag="POH73_000890"
     CDS             complement(17705..19060)
                     /locus_tag="POH73_000890"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDI6886861.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIVLDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(19087..20019)
                     /locus_tag="POH73_000891"
     CDS             complement(19087..20019)
                     /locus_tag="POH73_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HDI6886862.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKITGGEEQLNDIAYLKATFKTNKKINGDTKDIAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVSKAEPSETKAYINVK"
     gene            complement(20021..20830)
                     /locus_tag="POH73_000892"
     CDS             complement(20021..20830)
                     /locus_tag="POH73_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDI6886863.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            complement(21020..21928)
                     /gene="rocF"
                     /locus_tag="POH73_000893"
     CDS             complement(21020..21928)
                     /gene="rocF"
                     /locus_tag="POH73_000893"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HDI6886864.1"
                     /translation="MTKKKGIDIIGVPSTFGQRKLGVDLGPTAIRYAGLIPRLKQLDL
                     DVNDKGDIKVSAVDIEKFQSEQNGLRNYDEIIDVTQKLNKEVSASIENNRFPLVLGGD
                     HSIAIGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLTYRESHFALELLHQSQSVTSMDLVEVN
                     PLIDHNNHTAEQSVSLVGTFFGETLL"
BASE COUNT         6307 a         3993 c         3514 g         8253 t
ORIGIN      
        1 gtaaattaaa taataaataa ctgccgatgt tattaatcag ctaaaaaatg ctttgctaaa
       61 tattttgctg cgccatcctc ttcattcgta taagccgtta catctgaagt taatgcttgg
      121 atttcaggac gtacattttt catagcgact gtgtaatggc caaattcaaa cattgctcta
      181 tcattgtcgc tatctccaat aactaacgtt tctttttgat tgatgccaaa atgttcaatc
      241 atttctttaa tgcctgtacc tttatcagtt tgataagcca ttgtttccgc attaaatctt
      301 gatgaatttg aaacactaat ctgtagttgc acatgatttt gctttaattc atctctaaat
      361 gctgttattt tttctaaatt agaactgaac agataaattt ttgaatattc accttcagga
      421 aacttagtta cccaatctat cttaccagca agcgcatctt gtcttgaaga ccattcacta
      481 tgacttacgc cattaatagg atcttgacta cgaatcatat ctcgcatcca tgtttcatct
      541 tcttttaaag aaactctatt accttcaaaa ggaaatacct cataataaat ttgttggcgc
      601 ttagctaaat taataatttg ttgcacttga gccaatgata aaccatgttt gaagataatt
      661 tctccatcta cttctccaat ggttccattt gaactaatga tgccatttac cgcaaaatct
      721 tcaggtacaa gttgatgtat ttcagaatgc gaacgtccag tcgctaaaaa tactttatat
      781 cctttctctc tcaatcgatt aatgacatct ttcgtatacg ttgatgcttg attattttca
      841 tgtaaaattg taccatccat gtccaaaaat attgctttta cgttatccat attgccctcc
      901 actgtcaatt ttagcttaat taaattttgt cattattagt tagtatttgc aaaatattta
      961 ctctaacatc gccatagaaa atcgattatt ccatctttga actgctacat ttttctggaa
     1021 aaatcgtgac atgttttcag atgtcaaaat atcatctatt aacccttgtt gtacactttc
     1081 cccatctttt aatagcaaaa ttttagtaaa gttaccagta atttcttcaa taaagtgcgt
     1141 cacataaatc atcgcaagcg ttggatatga atctgacaat gagtcaagta tacttaacaa
     1201 cgattctcgt gcaataaagt ctaaaccagc tgctggctca tctaaaatta aaacctgggg
     1261 ttgccccatt aaagctcgcg caatcatcac tcgttgtttt tcaccggtag ataaataacc
     1321 aatatattgt tgtgctttag cagacattcc aactaattta agtaattgat gtgcttcatt
     1381 acgtacctca tcatcaatat cttgataaac accaattgat ttaaaagcac cgcttatcac
     1441 tacatcgatt actctttcac cctcttgaaa cttttccagt aaactatgag atacaaaacc
     1501 tatatgttgt cgtacagttt ctgtagaata ccctaccttg ccgggcattt taccaaatag
     1561 attaacatag ccagttgtcg caggctcata cgcatttaaa atatttagaa gtgttgtctt
     1621 gccagcacca tttaaaccat atagaatcca tttatcacct tcattaattt gccaagaaat
     1681 attttttaaa attgtttttc cttgcttcat tcgcccaact tgatctaatt gaattaacat
     1741 gcgatgccac ccctttattg ctactccata cttctcataa aatcatatta acatatcact
     1801 cttaattgtc agactattta aataaataaa ctacttcatt tttacgcatt tctgccgaat
     1861 taagatgaag taaacgctaa gtcgacctaa aaaagcaccc ttttagtcga ttaatctaaa
     1921 agggtgccat atactttaat tttaatacat gattgattct aaaaaagtga attattccac
     1981 agtaactgat ttagcaaggt tacgtggttt atcaacatct aaatctctgt gtaatgctgc
     2041 atagtatgaa attaattgta atgtaacaac tgatactaat ggcgttaata attcatgtac
     2101 atgaggaatg acataagtgt cgccttcttt ttcaaggccc tccatagaaa taatacatgg
     2161 gtgtgctcca cgtgctacta cctctttaac attaccacga attgataaat taactttctc
     2221 ttgtgttgct aaacctacaa ctggtgtacc ttcttcgatt aaggcaattg taccgtgttt
     2281 aagttctcca cctgcaaaac cttcagcttg aatgtaagag atttctttaa gttttaacgc
     2341 accttctaaa cttacgttat agtcaatagt acgtccgata aagaatgcat tgcgtgttgt
     2401 ttctaagaaa tctgtagcaa tttgttccat gattggtgca tcgtcaacaa tcgcttcaat
     2461 tgctgttgtt acttttgcta attctctcaa taaatcaata tctgcttcac gaccgtgctc
     2521 ttttgcaacg atttgagaca agattgataa tactgcaatt tgtgcagtat atgcttttgt
     2581 agatgcaact gcgatttcag gacccgcgtg taataacaat gtgtggtctg cttcacgtga
     2641 taaagttgaa cctgcaacat tagtgattgt taatgattta tgacctaatt tattagtttc
     2701 aactaatacg gcgcggctat ctgcagtttc acctgattga gaaatataaa cgaacaaagg
     2761 tttttcagat aataatggca tgttgtagac aaactcagat gcaacgtgta cttcagttgg
     2821 tacgccagcc catttttcta aaaattcttt acctactaaa cctgcatggt agcttgtacc
     2881 tgctgcaata acgtaaatgc ggtctgcttc tttaacatca ttaatgatgt cttgatcaat
     2941 tttcaagtta ccttctgcat cttgatattc ttgaataata cgacgcatta ctgctggttg
     3001 ttcatgaatt tcttttaaca tgtagtgtgc ataaacacct ttttcagcat ctgatgcatc
     3061 aatttcagca atatatgaat cacgttctac aacgtttcca tctgcatctt taataataac
     3121 ttcatctttt ttaacaataa cgatttcatg gtcatggatt tctttatatt cgcttgtcac
     3181 ttgtaacatt gcaagtgcgt ctgatgcgat aacattgaaa ccttcaccaa cacctaataa
     3241 taatggtgat ttatttttag caacatagat tgtgtctttg tcttcagcat ctaataaacc
     3301 taatgcatat gaaccatgta ataatgacac aacttttgta aatgcttcct cagttgaaag
     3361 tccttgattt gaaaagtatt caactaattg aacgataact tctgtatctg tttctgaaat
     3421 gaatgataca ccttgtaagt attcaccttt taactcttca tagttttcaa taacaccgtt
     3481 atgaactaga gtaaaacggc catttgatga ttgatgtgga tgagagtttt catgattcgg
     3541 tacaccgtgt gttgcccaac gtgtgtgacc gattccaaca ggaccatcaa aatcgctact
     3601 atcagcaact ttacgtaatt ctgcaatacg acctttttct ttgaatacag ttgtattatc
     3661 atcatttact actgcgatac ctgcagagtc ataacctctg tattctaatt tttctaaacc
     3721 ttttaataat aattctttgg cattatcata gccaatataa ccaacaattc cacacataac
     3781 gacattttcc tccatattgg aatagtacgc gtaaattatg atttattgcc gataatttag
     3841 attgacaatc tgctttcata atataaatag gaacatgcta tcatcgcatt catccataac
     3901 aaattaagca tagttatttt tacaactatg caaattgctc acactgtact ttccatatta
     3961 atatttttta tattcaattt ctggcgatct tattaacttt gtccattaag tcaccctaat
     4021 gttttactta ataagctaac gaatgagcca catccgggat agcatccgcc gatctattcg
     4081 atcactatcc tcctcgtcta caaatacata ttttgcactc tataaaggcc actcatatat
     4141 taacctttaa tcttcaaata caaatattta tttgcacagg cgctttaact gtactgccga
     4201 actttccccc tttccattaa tcattattgt acaacggtgt tgttttgttt tgcaaatatt
     4261 ttcacaataa aattttaaaa atcctaaaac aatttttttg ttttactttt tcaaaatatc
     4321 tacgcagtca cattgatgac actttattta attttgtcac atttattttg acaaagttga
     4381 tttttgttta tattgaggat tgcgttatgg ctctttacac taaagatagt cacatacatt
     4441 aaaaaagagg tgtaaatatg tcgcaaactg aagagaaaaa aggaattggt cgtcgcgttc
     4501 aagcattcgg atcatttttg agtagtatga ttatgccaaa cattggtgca ttcatcgctt
     4561 ggggtttcat cgcggccatt tttatagata atggttggtt cccgaataaa gatttagcta
     4621 cgttggctgg tccaatgatt acttatttaa tcccactatt gattgcattt agtggcggtc
     4681 gtttaattta tgatttacgt ggtggtatta tcgctgcaac ggcaactatg ggggtcatcg
     4741 ttgcattacc tgatacacca atgttacttg gagctatgat tatgggtcct ttagttggtt
     4801 ggttaatgaa aaagactgac caattgatcc aacctagaac accacaaggt tttgaaatgc
     4861 tatttaataa tttttcagct ggtattttag gatttatcat gacaattgca ggatttaaaa
     4921 ttttagcacc acttatgaaa tttatcatgc atattttatc agtggcagtt gaagcattag
     4981 tacatgcaca cctacttcca cttgtaagta ttttagtaga gcctgcaaaa attgtatttt
     5041 taaacaatgc gattaaccat ggtgtattca caccgcttgg tgcagatcaa gctgcaaaag
     5101 ctggtcaatc tattttatac acaattgaat ctaaccctgg accaggttta ggaattttac
     5161 ttgcatatat gatttttggg aaaggtactg caaaagcaac ttcatatggt gctggaatta
     5221 tccacttctt cggtggtatt catgaaattt atttcccata tgtattaatg cgtcctttat
     5281 tattcatcgc tgttatttta ggcggaatga ctggtgttgc aacttatcaa gcaacaggat
     5341 ttggatttaa aagtcccgcg tcacctggtt catttatagt ttactgttta aatgcaccta
     5401 gaggtgaatt cttgcacatg ttactcggtg tcttccttgc tacacttgta tcatttgtcg
     5461 tagcagcttt aattatgaag ttcactaaag aacctaaaca agatttagaa gctgcaactg
     5521 ctcaaatgga aactactaaa ggtaaaaaat ctagtgttgc atctaagtta gtatcttctg
     5581 ataaaaatgt taatacagaa gaaaatgcta gtggtaatgt tagtgaaaca tcttcattag
     5641 atgatgatcc tgaagcgcta ttggataatt acaacactga agatgttgat gcacacaatt
     5701 acaataatat aaatcatgtt atttttgcct gcgatgcggg tatgggttct agtgcaatgg
     5761 gtgcaagcat gttacgtaat aaatttaaaa aagcgggcat taatgatatt acagttacaa
     5821 atactgcaat taatcaattg ccaaaagatg ctcaattagt tatcactcaa aaaaaactaa
     5881 ctgatcgtgc aattaaacaa acaccaaatg ccatccatat ttcagtggat aatttcctta
     5941 attcaccaag atatgaagaa cttttaaata atctaaaaaa agatgatcaa gcataataat
     6001 taaataaatt aaaaaatgga ggataccgcc atgttattga gtacacgtga aaaagaaatg
     6061 atagccctat tgattaagta ccacggtcaa tatatcacta tacacgacat tgctcagcaa
     6121 cttgcggtgt cctctcgtac tattcaccgt gaattaaaag gtgttgaagc atatttaact
     6181 tcattttcat taactttaga acgcgcaaac aaaaaagggc tacgcattac tggcgcagat
     6241 tctgatttaa acgatttgaa gcaatcgatt gcacaacatc aaaccattga cttatctgtt
     6301 gaagagcaga aagtaattat tatatacgct ttgatacaag ccaaggagcc agttaaacaa
     6361 tatagtttag cgcaagaaat tggcgtttct gtccagactt tagcaaagat gttagatgat
     6421 ttagagctcg atttaaataa gtaccaacta tctttatctc gaaagcgtgg cgaaggtatt
     6481 tacttggtag gtactgaatc aaagaaacgt gaatttttaa gtcaattaat ggtgaataac
     6541 ttaaatagta ctagcgttta ttcagtaatt gaaaatcatt ttgtctttca ttcattaaat
     6601 caaatccaca aagactttgt tgacttagag cgtattttta atgttgaaag actattaatg
     6661 gactacctaa gtgccttacc ctaccaactt accgaatcaa gttatttaac tttaactgtc
     6721 catatcgtgc tatccatttc acgtataaaa aatggagagt atgtcgcatt aaacgatgat
     6781 atttatgatt ctgtacaaaa cacatttgaa cacaaagtag caagcgaact tgctgataaa
     6841 cttggtcaaa tatatgacgt cacgtttaat ctggcagaaa ttgctttcat tactatccat
     6901 ttacgtggtg ctaaacgaaa aaatcttaat gatacatcat taaataatcg ttgtgaagaa
     6961 aacaaaatta aagcgtttgt taaaaaagta gaaatgattt caggtatgac atttgcagat
     7021 ttggatactt tagtagatgg actgacgcta caccttaatc ctgcaatcaa tcgtttgcaa
     7081 gctaatatcg agacctataa tccgttaaca gacatgatta agttcaaata tccaagactg
     7141 tttgaaaatg taagattagc tttaaatgat tgttggcctg atttgatttt tccagagagt
     7201 gaaattgctt ttatagtttt acactttggt ggctcgatta aaaaccaagg taatcgattt
     7261 ttaaacatat tagtcgtttg cagcagtggt atgggaacta gccgtctatt atcaactcgt
     7321 ctagaacaag tttttagtga gattgagcgt attacacaag catcagtcag cgatttgaag
     7381 tcactagatt taagtcaata tgatggcatt atttctactg tgaatttaga catagactcc
     7441 ccctatttaa cggtaaaccc attattacca gatagtgata tcagttatgt cgcacagttt
     7501 ttaaatacaa agtctacgtt ccaagagacg cgcgataaat catcaaacat gattgataag
     7561 gatgatgttc atattgaaac gaaagatatt gatggcaaca catcttttga aaatgaacaa
     7621 acttcatact taacttcagt tttcgaaaaa catttaagtg acgaaaaatc agaacaatta
     7681 atgcatcata tgcgttcggg tttaactttg cttgattcag tgaaaatagt tagtaccgaa
     7741 gttaaacagt ggcaaacata tatcgcagat tatctatatc aatgcgatgt aataaacgat
     7801 ccaacgtcat tcgctgaact actagagcaa cgattgattg acaatccagg atggatatta
     7861 agtccatatc ctgttgcaat accacaccta agagacaata tgattaaaca ccctatgatt
     7921 ctaatcacag ttttagaaga accgttaaca ttgcctagta ttcaaaatga caatcaaaca
     7981 attaaatata tgatttccat gtttattcct gacaatgatt ttatggcatc actgataagt
     8041 gacttgtccg aatttttaag tttgaaatta gaatctattg atacttttat ggaaaatcca
     8101 caggaacttg aaacattatt aagaaacaaa tttttagaac gaattaaaaa acaatttatt
     8161 taggagtgta caaaatgagc gaattattta gtaatgacaa tatcttttta aatgtaaatg
     8221 ttaacagcca aaatgaagca attgaaaaag caggtaaagc cttagttgat agtggtgctg
     8281 taacagatgc ttatattcaa gcaatgaaag atcgtgagca agtcgtatca acatttatgg
     8341 gaaatggctt agcaattcct catggcacag atgaagctaa aacaaatgtg attcactcag
     8401 gtttaacatt attacaaatc cctgaaggcg ttgactggga tggcgaagta gttaaagttg
     8461 tcgtgggaat cgctggtaaa gatggcgaac atttagactt gttatctaaa attgcaatta
     8521 catttagcga agaaggaaat gtagaccgta tcgttcaagc aaaatctgca gaagaaatta
     8581 aacaagtttt cgaggaggca gatgcataat gaaagcagtt cattttggtg ctggtaatat
     8641 aggtcgtgga tttattggat atattctagc agataacaat gtaaaagtaa catttgcaga
     8701 tgtaaatgaa gaaatcatta atgcattagc tactggacat caatatgatg ttattttagc
     8761 tgatgagtct aaaacaacaa cacgtgtaaa taatgttgca gcgattaact caatgcaacc
     8821 ttctgaagaa ttgaaacaag caattttaga agcagatatt attacaacag ctgttggtgt
     8881 taatatacta cctattattg ctaaatcttt tgcgcctttc ttaaaagaaa aaacaaacca
     8941 tgttaatatt gttgcttgtg agaatgctat tatggcaact gatacattga aaaaagcggt
     9001 acttgatatt actggacctc ttggtaacaa tattcatttt gctaactcag cagttgatag
     9061 aattgtacca ttacaaaaga atgaaaatat attagacgtt atggttgagc cattttatga
     9121 atgggttgtt gaaaaagatg catggtatgg tccagaacta aaccatatta aatatgttga
     9181 tgatttaaca ccatatattg agcgtaaatt attaactgtg aatacaggac atgcatattt
     9241 agcgtatgct ggaaaatttg ctggtaaagc tacagtttta gacgctgtaa aagatagttc
     9301 aattgaagct ggcttacgcc gtgttttagc tgaaacaagt caatatatta ctaatgaatt
     9361 tgattttact gaagcggaac aagctggtta tgttgaaaaa ataatagacc gtttcaacaa
     9421 ttcttattta tctgatgaag taacacgtgt cggacgaggt acgttacgta aaattggccc
     9481 taaagataga attataaaac cattagcata tctttataat aaagatttag aacgcactgg
     9541 tttattaaat acagctgcat tgttattgaa gtatgatgat acagcagacc aagaaactgt
     9601 tgagaaaaat aattacatta aagaacacgg tttaaaagcg ttcttaagcg aatatgctaa
     9661 agttgacgat ggcttagccg atgaaataat tgaagcttac aattcacttt cataatttat
     9721 tgagctttgt ttgaaactat aattttccaa cgttattcgt taacaatcag taataatgta
     9781 gtagttccgt tgaattaaca atattacatt tctgaacata aaaaatactc ccttcaacat
     9841 agacacttaa cttgtgttat gtatgaaagg agtatttttg cgttaataat ttgttttatt
     9901 ttcgagccac agccacctat tcaatggcta ttggtcatta ctaaaacaaa ttcatattaa
     9961 ctgttagact tggttactta gtaaggaata tttccctatg aaataactag atgttcacat
    10021 tcttaaataa attttattct tcagtttgtt ggtctttctt agtgaatctt ctaattaaga
    10081 atgccatacc tgcacctaga gctaattcaa catatggtaa atcgtcatta tgtgacatac
    10141 cagtatctgg taaagtttta gcttgttgtt ttgctttatt ttctttacct tgttgcgctg
    10201 atgttgcctt agcttggtgt ttgtcagtgt tagttacttt aagcatatct tgattagcac
    10261 tattgcttcc atttgaaact gtagctggag atgcattggc accgtcgttt tgcgtagctt
    10321 tattgtttac atctgcagtt gcaccaattg ctgcttgatt atctttttta tctgatgttg
    10381 ccatgtcagc cttttgacta tcctcagtcg aagtcgcttt atcttttgct tctgacggtt
    10441 tagatgttga tggtttattc gatacatcag tatcaacgtt gccttcagat ttatctgctt
    10501 cgttagatgc aacgttagtt tcagacttaa gttgtccagc atctgtttga tttgtcgtac
    10561 tttctttttt atctttctct gtattagaag gtacttttgg ttctgtaatg tctgctgaag
    10621 gcaatgtttc agttgttgat tcaaccatac tttgatttgt tgaatctcta ccatcttttt
    10681 catccttagc tttattttca gattttggtt gtgcaacctt gccattagtt gattgagatt
    10741 cagcactatt atttacttca gcattttgtt ttgaatcatt tacagatgca ttatctttgc
    10801 tatcagcaga tgatgctgca cctgtgctcg catttgttgt tggaaccgtt gctgttgatc
    10861 ctgttggtgc gttctcgttt gttgcagttg tactattgtt atttcttgcg ctttctgctg
    10921 gcgttgcatt atctgtttct gttgcagatt tatcagttgt gccgttatta gttgattcta
    10981 cttctgattt atcacttgta ccattacccg ttgccgaatt gtctgttgat gttgctgttg
    11041 ttccaccagt tgccgcatca ttatgattat ttgtcgttgt tgaagacgta tctgcattgg
    11101 cattatctgt tgactcagtt acattaggtt gtccagtatt ttctggtgtt gcattagcat
    11161 ttgaatttgc tgttgcatca ttattatctg aaccactatt agtatcatta gcatttggat
    11221 ccttctgagg tacaatcgct tcaattgcag gtatcgttac attttgtaat tcatcaactt
    11281 ctgtatttgt ttgcgtttta tctaatttat cagcaaatct gtcaaaatat ctacctaaat
    11341 ctgtacgtgc aatttctttc gccgatgcat ctgcattttc aattatttct atttgcttgt
    11401 taaccacttc tctaattgct tccaaagcat ttttcttaac tactggatta atacgttgtg
    11461 ctttaagttg ttcaagcgca ctatttttga cagtagcgat ttctgcattt gtagtttgat
    11521 cagaaatatc ttcagttgct tttgataaaa tgtcttctaa tgcattcgta aacgcttctt
    11581 tttcttcagt tgtagcatca gcgtcgacat ttactcctgc ttcaatctgg tctagtgcag
    11641 tttctaattc ttcgatagct ttttgttttt ctgttgagtc aatttgaatg ttatcaaacg
    11701 cctcaagtcc ttgagctttc gctttttcaa cttcagcagt tgttgttgca tcagtaatac
    11761 cttgtttagc ttgatctgta atttgtttaa tcgttgctaa tgcagccgtt ttttcttcag
    11821 cagttaactg gtcactttgg tctatagact ctttcgtttc ctgtgcttta gcatcaatcg
    11881 cttggtttgc tttaggttta acagtagcat ctacttgaac tgcatcaatt gtcgctttac
    11941 cttgtgttaa tgcatcatca acatcactat tatccacacc attattaatg ctttctaatg
    12001 cagtttgaac attttggtca acttgcttaa ttgcttgttg tttttcatct tgtgttgcat
    12061 tagtgtcagc taaaatatta tttttcttct gatctgcata ggcatataaa tctgttgtag
    12121 ccgattcttt tttaccggtt ggaattgtgt aatcgttaat attatctaag tcattatgaa
    12181 tttgaacttc aatgtcatct ttagaagtag cttgattaac attttgatcc gctgtttgtt
    12241 ttaattcagc aagtttttgt ttcgcttcag caatttcact tgaagtcgat gcgttagagt
    12301 tatccgcttc gttcacttta gcgttatatg catctgcaat tttagctaaa gcatcttttt
    12361 tgtactcact aaatgtttta actgcattaa ttttagcttt cccttcttta accgcattat
    12421 caacatattc atttgttgat gattgatcaa tatttgtttt cgcttgattt aacgctgcat
    12481 caacttcttg ttttgcatca ttaatttctt gttgtgacgc atttggtgtt tgttcaattt
    12541 gtgttttctt atcagctgca gcttgatcta attctgtttt acctgctggt ttcttaacag
    12601 gatttgcatg aagttgttga actttttgta ctgctgtatc tttagcagta gttacatcac
    12661 ctgtagtagt tgctgcatta atattattta aaccttcttc atatgctgct ctaactggtt
    12721 ctatatcttt tcctttttct tcatttgtag tcgtattatc attaaggatt tcagttattt
    12781 tattttgcat ttcatttagc aattccgctc ttgcattcgt tttaacatct gttgatgctt
    12841 gaattgggtc aattgcttga attgcattat cttttgcagt gtttacatca tcgattgact
    12901 ttgcattttc aatattttga ttaccttgcg ttaattgtgc atctacttgt tgatttgctt
    12961 gttctttttc ttcagtagtc gcatctgcag tttgtgcaat aagtgctttt tgttcctttg
    13021 cttttgttgc taattcatct ttagctacat ctttaattgt cgtatcagca gtaatacctt
    13081 gaatgtcagc aactgcttga tctttaattt gtgtaacatc gttagtcgtt tgcgcattta
    13141 aaatatcttg atatgctttt tctttagctt tagaaactaa atcttttgct gcatttttct
    13201 cttcagtcgt tgcacctgta gtattatcaa ttgcttgatt ttgagttgtc acagcttgat
    13261 ctacttcatt tttcgcattc gatttaactg ctgttgctgg ttgtgtactt tgaatcgaat
    13321 ttttacctgc atctttagca gtgtctacac cattattgtc agttgcagct gtaatgttat
    13381 ttttcgcgtc tgtaactgct gttgctagtt gttgaatcgc cgcttctttt tcttcatttg
    13441 ttgcgttttg atcgttatta ataaccgctg tttgtgctgc ttgtaattga tcgatctcat
    13501 ctttcgctgc ttgtttcttc ataacttttg gtgttacagc gttaattgcc gcttctgcat
    13561 tagtttttgc ctcatctact tctgcatttg ttgtagctgc tgtgatggct tgatttgctt
    13621 tagcattttc agtattagct tcagtttctg ctgcttgttt ctcttcatct gttgcatctg
    13681 gcgtagcttg aattgcttgt aacttgttat ttaatattgt agtgatttca ttacgtgctg
    13741 tagctttttt attaacagtt ggtgtcactt gatcaatact agcttcacct gttgtcttcg
    13801 cttgatcaac ttcattttga ctattagcag tctcaatgtg gttgtttgct tctgttactg
    13861 cattatctac gttcgcatta gccgctgcaa tttcttctgc tgtaatctct tgcgtttgag
    13921 caatggctgc tttacgttca tttgctttcg tagcaatggc ttgtttcgca tcatctttcg
    13981 ttgttgtcgc tggttgaatt gcttcaattt tagcaatttc tgcattctta gccgcttcaa
    14041 cttctgcatt tgaatgtgca ccatctattg ctgtatcagc tgtcgttttt tctgtttgaa
    14101 cttgttgttt cgctgctgtt ttctcttctg ttgttgcacc gttattagca tcaattgctg
    14161 tttcttgcgc ttgtacttta tctgcaattg cttgtttcgc ttgaggttta acatttgcat
    14221 caggtgtaat ggctgcgatt gtagcttcat ttgtagtttt tgcattatcc acatcattgt
    14281 ttgctgcggc attatcaata tcagcgtttg cattaactac tgcttgatcc actttatctt
    14341 tcgctgcttg ttgttcttca gtagtcgaat cattcattgc ttcgattgca gttttacgtt
    14401 cacttgcttt ttgagcgatt tctgctttag catccgattt cttagtagtg gcagcttgaa
    14461 cttgattaat tgcagcaata ccattgtctt tagctgtcgt tacagcactg tttgtatttg
    14521 cagcatcaag attcgttctt gcctcttgtt tttttgcatc taattgcgta tatgccgctt
    14581 ctttttcttc tgttgttgaa gtgttactat tttggatttc ttgtttacgt gtattatatg
    14641 cgttgtctac ttctctatcc gctgcaggtt taactctagc ttgtgtttga atggcattga
    14701 ttttatctaa tacttttgct ttagtatcag ccacttcctg ttttgattta acaacttgga
    14761 tgtcatgtaa accttctgtt tgagctgcat ctactgctgc atcagcttct gctacttctt
    14821 catcagttgc atttgaaact gtagcatttt gagcttttct agctgtagca gcttgtttaa
    14881 cttcattata ggcatccatt tttgtttgcg tagagtccgt aatattctca attttgaata
    14941 tttcttcgtt tttaactcta tctaaatctt gtgcagtcgt tgtcgcttca attgctttaa
    15001 cacctgaaac tttagctgca ttaatacgtt caatcgcttc cgctacctct tcatttgttg
    15061 tatcaggatt taaaggtgct tgatcaattt gtgcttgaac aacttcgtca aattcttcaa
    15121 gagctgctgc tttagctgtt gtagcaggtg cagtttgatt aatatcattg atcgcttttg
    15181 ttttaatggc ttctacttgt gcatttgttg ttgctgcatc aatatcttta attgcctttg
    15241 tttcaatttt accaatttta tcatttgcta catctttttc atcttgtaat gatgcatttg
    15301 actttttaat ttgttgttta cgagttgtaa ctgcttggat aatatcttgt ttcgcttgag
    15361 gtttaactgt tggtgtaatc acatcttgtt ccaacactgc gataccatta tctttttcag
    15421 ttgtgacacc ttgtgctgtt gtttgtttat taatttctgc gattgcatta tttttatcat
    15481 tgttaatttt agtcacaagc gctgcaactt catcttgcgt aactttttta ctttgttgtg
    15541 ctgctgtaac cttttcttga gctttagcat ctaattcaga aattgctgct tgattatcaa
    15601 ttaatgcagc ttgtaattta gttactaaac catcaatatc cgcttgagat gcacgatttg
    15661 cagtttcaac atgattcgca tcttcattta aaatagtgtc tgcttgttgt ttaagtttat
    15721 tgtattctgc aattgatgct gttgtgtaat gactatcatc aacttgtgag ttaacttgtt
    15781 gttgcaacgc atctttgttc atttcaactg ctactgaaaa tggatctgca gttagtgtaa
    15841 cttctggttg cgcattatta attacaacat ctgaagcagt acgatatgtt aatttttcat
    15901 taaaatcaat atttttaggt gtatcaatat tcgcaacatt aactttatat gataatttta
    15961 aagatttatc tgggaataac acttctttcg tatgtgtacc acgtgctgtt gtcacacctt
    16021 ggcttgtaaa tgtaacttta tttgcatttt gatcataatt gacagtcata tttttcaata
    16081 ctgtactgtc ttcattacca ttagggaatg ttgtagttaa tgaatcatta acgtaagtta
    16141 caccttgtgg taatgttact tcatatttaa attgatctgc tttcaatgaa gcaccaaaat
    16201 taccattgtt accgatttca gtattaatag tatattcttt attgttagtt gctttcggtg
    16261 ctaaatctct atttttgaca tataaatgtg atcctgagaa tagacctaca ttatcaatga
    16321 atgaatagta tttataacca tctttatttg ttgtaattct tgaagcatca gcaactgctg
    16381 tattgtcagg aataaattct aaatctaaac gatcagcatc tgttggtact ttaaataagt
    16441 gtgcagtacc agtcttagct acatttacag ttgctaattc agtatttttc ttagcatctt
    16501 taataactaa atttgttgca ccttgcttac tattcgtttg catagtagta aagttgataa
    16561 ttaattctga atctggtttt acattaacag tttttgaaat accgttaaag ttaccgtgat
    16621 ctgtagaatc atttgcattc acacgaccta atgcagctac attccctcta gcttggtagt
    16681 tttgagggtt attcttatca aacatatcgc ttcgtcttaa ttctgagtta acgaaaccaa
    16741 tcttaccgtt gttaattaat gaataaccat ttactttatc tgtaacagtt acagttggat
    16801 cctgtctatt ctcatctgtt gatatggcag gatcatcaaa cgttaatgtt gtattaatac
    16861 tgccttcacc agtattgcta gcatttggat cttgagtttg tgcgtttgct gctacaggtg
    16921 ctgctggttg cgctgctgct ggagcattcg ctggctgtgt ttgatttgcc ggtgttgtat
    16981 tattattagg tgttgcttgg ttatttcctt gacctgcttg gttggcaggt gctgcttgat
    17041 ttccaggttg tgcttgtgca gcgttattcg gatcagcttg attaccttgt ccagctggtt
    17101 gtgctgtacc tgctgccggt tgtgctgcgc ctcctgctgg attagcctgt ccaccttggt
    17161 ttgctggttg tgctgctggt tgtccttggt tggcaggtgc agctggctgt gctgtaggat
    17221 tagcttgagc accagcattt gcgttaggct gtgcattggc atcagctggt tgtgctggtt
    17281 gattttgtgc aggctgattt tactctgctg cagacgctgt tgtcgggtta atagatataa
    17341 atgtaacagt ggcaattaaa gctgaaaaaa taccgacatt aaattttcta atactaaatt
    17401 tttgttgtct gaataaattc attaagtcat cctcctggtt gattattctc gctgttaaat
    17461 gatgtcactt aatcaacttt taagataaac agtttgcttc catcttataa aaggtatagc
    17521 aactctatct aattaaaatt aattttatca tcattatata ttgcttacca gtgaatttta
    17581 tattacatat tgattacttt gtttttatgt attctttatt attttgactt tacgaaataa
    17641 tattcttgct ttagtcaaat taaaaaaccg atgcataagc atcggtttga tttatatctt
    17701 gaaattattt atctaatccc attttatctt gaaccacatc agctatttgt tgtgcaaatc
    17761 tttcagcatc ttcatcagtt gctgcttcaa ccatgacacg aactaatggt tctgttccag
    17821 aaggtcttac taaaattcga ccttctccat tcatttctac ttctacttta gtcataactt
    17881 ctttaacgtc aacattttct tcaacacgat atttatctgt tacgcgtacg ttaattaatg
    17941 attgtggata ttttttcatt tgtccagcta attcacttag tgatttacca gtcattttta
    18001 ttacagaagc taattgaata ccagttaata aaccatcacc agttgtattg taatccatca
    18061 taacgatatg tccagattgt tctccaccca agttataatt accgcgacgc atttcttcta
    18121 ctacatatct gtcgccaact ttagttttat tagatttaat tccttcttgt tcaagcgctt
    18181 tgtaaaaacc taagttactc ataacagtag aaacaatcat gtcattattc aattcttgat
    18241 ttttatgcat ttcttgacca ataataaaca taatttggtc accgtcaacg atttgaccat
    18301 tctcatctac tgctatgatt ctgtctccat cgccgtcaaa tgctaaccca aaatcacttt
    18361 cagtttcaac aactttttct gctaattttt caggatgtgt agagccacat ttctcattga
    18421 tattatatcc atcaggacta catccaattg tttcagtatc tgcctctaaa tcaccaaata
    18481 agaatggcgc tagtgatgat gttgaaccat ttgcaccatc taaaacaatt ttcaaacctt
    18541 caaagttaac atctactgtt gattttaaat aactcaaata tttttgtgcc ccttcaaagt
    18601 aatctgaata atgtacaata tcattgccaa ctggtcttgg taattctggg ttttcttgat
    18661 ccaataatgc ttcaatttca ttttcttgtt catctgatag tttaaaacca tctgatccaa
    18721 agaatttaat accattatcc gcaactggat tatgagaggc tgatatcatt acacctaact
    18781 ctgcacccat atcgcgtgtt aaatatgcaa cacctggtgt tgaaataata cctaatcgca
    18841 tcacttctgc accaattgaa atcaaaccag ctattaatgc tgattctaac atttcacctg
    18901 aaactctagt atcgcgacct acaagtacac gtgggtgttt ttcaccttta ttatgtgcta
    18961 gaacatagcc accatatctt cctaatttaa atgccaattc aggtgttagt tcttggtttg
    19021 cgacacctct tactccgtct gtaccaaaat attttcccat tgtttactct cctttaattt
    19081 agctatttat tttacgttta tataagcctt cgtttcactt ggttctgctt tagagacatg
    19141 ttctggtaaa ttgattttta cagtcttttc agttgattct gaaataccat ctaaatctac
    19201 ttctgcatca acttcgctta tattttgcaa gtcatctcga ctaccgaaga tttcaatttc
    19261 tttatcttct aagtcaatcg aacttaattc tttatcatct gctaaactac ctttctgttt
    19321 aacatttact ttaacctttt tgctaaaagg ctctacttta acttgtaaat tcacttcatt
    19381 aggttgaatc gatacattta atttattcag ttttttatca aaagccgtta cttccgcgat
    19441 atcttttgtg tcaccattaa tctttttatt agttttaaaa gtggctttta aataagcgat
    19501 atcattcaat tgttcttctc cacctgttat tttaactgtt tgtggtgaaa cttcttgctt
    19561 tgtaatttta taaagtggat caatatcact ttgactaaca tcaggttgaa ctgtcatctt
    19621 tttagttact ttgttttcaa gcgtaatatt tgccaattta ggttttacag aataatgaat
    19681 gtcatcagct aaccctttaa cttgatactt ctcttgatac tttccagctt tagcatttga
    19741 taaatcaatc actactctta aatcttctgg attttcaatt tttattatct ttgattgtgg
    19801 tcctgaaata gtcacattaa ctgtttcagg cgcttttgtt aaatgcaaat ctttagtgtt
    19861 ataaagaatt tcaacgggta catcttgaat cgttttacta gacttttgac caagattacc
    19921 agtgttaaag atatttccaa aaacattgtt aacagataaa aagaaaaaca atgccaaaag
    19981 aaaggcaata aatcttaagc cccatttact ttctagcata ttatttcaca cctttctttt
    20041 gaaagcgtgt gccaaaccaa tgttcagcaa gcaactcttc aaaaatttcg tttgaaatgt
    20101 ctcggcgtaa ttttccatca aatgttaccg aaatatcacc agtttcttca gatacaataa
    20161 cggtaaatgc atcagatact tctgaaatac caaccgctgc tctatgtctt gtacccaaac
    20221 ttttagatat cttaggacta tcagacaatg gcaaataact tgctgctgct gcaatcttcg
    20281 tgccttgaat aatcattgca ccatcatgta aaggtgtatt aggtataaag acattaatta
    20341 aaagttcttg tgaaatattt gaatccattg caatacctgt ttcaatataa tcttgaagac
    20401 ctgtttcttt ttcaaagaca attaatgcac ctatacgtct tttagccata tattgcacag
    20461 cctttgaaac cgattgaatc aatttctctt catctttact atacgtatta gaagtatagc
    20521 gttttaaaaa gctacctcta ccaagttgtt ctaacgcacg tctaatttct ggttggaata
    20581 ttactattaa agctaatacc ccccattgaa taacgatatc gaataattta gatgttgcag
    20641 tcaagttcaa tatcatactt atctgctgac caataacaat tactaatatc cctttaagta
    20701 attgtatcgc tttagttccc ttaaagaccg tgatgagaag ataaagtaca taccaaacta
    20761 tcagtaaatc aaggatactc gttacaattt ttaacgtact gaggttttga aaaaagttgg
    20821 aaaaatccat aacatctcct ccgggtaata tttttccata atacccatta taccaatcat
    20881 ttacatcact gtcatacgaa catatattta aatagaaaaa aatttatttt aaagattata
    20941 actactctta atcattttag tgaattaaaa aaagtagtgc aaaaagcaaa atatacttta
    21001 tacactacaa atcatttatt tataataaag tttcaccaaa aaatgttcca actaacgaaa
    21061 ccgattgttc agcagtatgg ttgttatgat caataagtgg attcacttca actaagtcca
    21121 ttgaagtaac tgactgagac tgatgtaata attccagcgc aaaatgactt tctctatatg
    21181 taagtccgcc taagactcta gtacctgtgc ctggcgtttc aagtggatct aaggcatcaa
    21241 catctaaaga taaatgaacg ccatcaacat tgcgtgactt caaatattct attgtatttt
    21301 caattacttc ctttatcccc aatttatcaa tatctgacat agtaaatgtt ttaatattat
    21361 gatctttgat aaattgtctt tcacctttat ctaaatctct cataccaatt agtacgatgt
    21421 tttctggctt gattacatta ctatttaatt ctaaaagttc tttaggacct tcacctgtca
    21481 aaatccttag aggcatacca tgaatatttc cacttggtga ctcttcaggt atatttaaat
    21541 caccatgtgc atcataccaa ataacaccta aattattata atgtttactt attgctgata
    21601 ctgaacctat cgcaatagaa tgatctccac caagaactag aggaaatctg ttattttcaa
    21661 ttgatgctga tacttcttta tttaattttt gagtaacatc tataatttca tcgtagtttc
    21721 ttaatccatt ttgttcactt tgaaattttt caatgtctac agctgatacc ttaatatccc
    21781 ctttgtcatt aacatcaagg tctaattgct ttagtcttgg tattaaacct gcatatctaa
    21841 tcgcagttgg tcctaaatca acacctaatt ttctttgtcc aaatgttgac ggtacaccaa
    21901 taatatcaat tccttttttc tttgtcataa gcgtccccct ttgttataca taatttcaga
    21961 ataatgtata cgcttactat aatcaagtaa tagccataac ataacgcctt agcatcctaa
    22021 ttctcaaaaa ataaaaaaag cacttcccaa gaatggaaag tgcaagt
//
LOCUS       DAMZPP010000031        22018 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMZPP010000031 DAMZPP010000000
VERSION     DAMZPP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22018
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.031"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..213
                     /locus_tag="POH73_000894"
     CDS             <1..213
                     /locus_tag="POH73_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886865.1"
                     /translation="KVKGMIPGVNAPPIHPWCRSTTVPDVGNWRDKFFKEREGKYRVE
                     NNTEMKLGEVHYSKTGFHVVPYIKKE"
     gene            220..417
                     /locus_tag="POH73_000895"
     CDS             220..417
                     /locus_tag="POH73_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HDI6886866.1"
                     /translation="MNLQSYIGKLVKLTLTNNKILIGKVIDFDDKIDNFDGYNSIEID
                     TGRITYDISENKIKDIQIITK"
     gene            complement(964..1989)
                     /locus_tag="POH73_000896"
     CDS             complement(964..1989)
                     /locus_tag="POH73_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HDI6886867.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFIN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
     gene            2259..2456
                     /locus_tag="POH73_000897"
     CDS             2259..2456
                     /locus_tag="POH73_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886868.1"
                     /translation="MEQIKLKTFTSETLELLESNINEFLGSEEAANLKLVNITIKEIE
                     ERTFPNNEEEFNAILTLSVNK"
     gene            2513..3994
                     /gene="cls"
                     /locus_tag="POH73_000898"
     CDS             2513..3994
                     /gene="cls"
                     /locus_tag="POH73_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDI6886869.1"
                     /translation="MQFSFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIIIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTEESYANRPLSVKFKESLAKLVSPIL"
     gene            4168..5076
                     /locus_tag="POH73_000899"
     CDS             4168..5076
                     /locus_tag="POH73_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6886870.1"
                     /translation="MVHLIQISNINKSFKKNRVLKNISFDIEKGTCTALIGKNGAGKS
                     TLVDILSNKIIADDGVILDKDKLLQSENRSIMFQKTMFPDQLKVIGIIKLYQSFYENP
                     LTLDVIIELTKFNSNQLNQFANKLSGGQQRLLDFVISLIGQPQLILLDEPTSNMDIEM
                     REYFWSIIAKLKEENRTILYTSHYIEEVERMSDKIILIENGEIKLNDSTSHIRTNQQS
                     QITLSDEYKRKLKPDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKV
                     SIVDSYFNNKKQRGSNYDTKLLENRI"
     gene            5045..5776
                     /locus_tag="POH73_000900"
     CDS             5045..5776
                     /locus_tag="POH73_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6886871.1"
                     /translation="MILSYLKIEFKVILRKKTTLILSILFPVIFYILFTSILDLPEDV
                     KPKFYKEYMYSMTVYSLLSFSLLTFPLDIINEKQNGWRQRLMVTPFTFTSYYISKVVK
                     TMLQFAIAILVIFMVGHFYKGVEMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQK
                     TSALANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLIS
                     FVIILLYALGSIIAVYCISHFKRAE"
     gene            5780..6871
                     /locus_tag="POH73_000901"
     CDS             5780..6871
                     /locus_tag="POH73_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI6886872.1"
                     /translation="MKFLKDTSIAEISSILYLIFPIAGIFFNEVYGPTWLYIISVIVF
                     SLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAIPFTFKN
                     NVKKMATNLFILTMIICFTITYLVHNDYFVAMTIYYVVILLIVFDNLKKVKDREYKKE
                     IEEKNRYINTLITEQERHRIGQDLHDTLGHVFASLSLKSELAYKIIDSDTENAKAELL
                     AINKLSRESLNKVREIIDDIRLPSFIEEIESICKILKDADIQFTFENKELAQLLSPTK
                     QSILVMIAREAINNVIKHANASKVHGQLRAMNEHKLQFIIQDNGEGIDSGCEIKSISQ
                     RVKHLNGTLEVESNKGTKLIIEMPTGGIA"
     gene            6868..7470
                     /locus_tag="POH73_000902"
     CDS             6868..7470
                     /locus_tag="POH73_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6886873.1"
                     /translation="MISLIIAEDQNMLRQAMIQLIKLHGDFEILADVDNGLDAIKIIE
                     AHHPDVVILDIEMSGMTGLEVLSEIRKKHLNIKVIIVTTFKRPGYFEKAVANDVDAYV
                     LKERSIEELVETIYKVYNGKKEYSASLMTSFFTDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVRIDKLFADNRFDAWKKANEKGWI"
     gene            complement(7583..7771)
                     /locus_tag="POH73_000903"
     CDS             complement(7583..7771)
                     /locus_tag="POH73_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HDI6886874.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            7910..8443
                     /locus_tag="POH73_000904"
     CDS             7910..8443
                     /locus_tag="POH73_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDI6886875.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHKGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            complement(8586..9440)
                     /locus_tag="POH73_000905"
     CDS             complement(8586..9440)
                     /locus_tag="POH73_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886876.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEMSNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     NQITAIFK"
     gene            9771..10346
                     /locus_tag="POH73_000906"
     CDS             9771..10346
                     /locus_tag="POH73_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886877.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDNTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            complement(10400..11782)
                     /locus_tag="POH73_000907"
     CDS             complement(10400..11782)
                     /locus_tag="POH73_000907"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDI6886878.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            11973..13253
                     /locus_tag="POH73_000908"
     CDS             11973..13253
                     /locus_tag="POH73_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDI6886879.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QAYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            13259..14320
                     /locus_tag="POH73_000909"
     CDS             13259..14320
                     /locus_tag="POH73_000909"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDI6886880.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSEILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPLR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            14322..15236
                     /locus_tag="POH73_000910"
     CDS             14322..15236
                     /locus_tag="POH73_000910"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDI6886881.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVAAKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVTKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     ALNRNVVTCHSELVDINVSGVKERIVYQ"
     gene            15294..16097
                     /locus_tag="POH73_000911"
     CDS             15294..16097
                     /locus_tag="POH73_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI6886882.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQDDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYADEITLDHNENGIPHALKKLL"
     gene            complement(16389..16703)
                     /locus_tag="POH73_000912"
     CDS             complement(16389..16703)
                     /locus_tag="POH73_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886883.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFTHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(16678..16782)
                     /locus_tag="POH73_000913"
     CDS             complement(16678..16782)
                     /locus_tag="POH73_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886884.1"
                     /translation="MLPFFILDILHIVYNIDNNNFSGVKFNDWTNKFI"
     gene            complement(16922..18376)
                     /locus_tag="POH73_000914"
     CDS             complement(16922..18376)
                     /locus_tag="POH73_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6886885.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMASIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHAYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPKFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVIATLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
     gene            18580..20097
                     /locus_tag="POH73_000915"
     CDS             18580..20097
                     /locus_tag="POH73_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDI6886886.1"
                     /translation="MSRQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEVAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            20188..20337
                     /gene="rpmG"
                     /locus_tag="POH73_000916"
     CDS             20188..20337
                     /gene="rpmG"
                     /locus_tag="POH73_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDI6886887.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            20791..21060
                     /gene="rpsN"
                     /locus_tag="POH73_000917"
     CDS             20791..21060
                     /gene="rpsN"
                     /locus_tag="POH73_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDI6886888.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYETLRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            21217..>22018
                     /gene="guaC"
                     /locus_tag="POH73_000918"
     CDS             21217..>22018
                     /gene="guaC"
                     /locus_tag="POH73_000918"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDI6886889.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKN"
BASE COUNT         7961 a         3053 c         3726 g         7278 t
ORIGIN      
        1 aaagttaaag gtatgatacc aggtgtgaat gcgccaccta tacatccttg gtgtagaagt
       61 accacagtgc cagatgtcgg taattggcga gacaagttct ttaaagagcg tgaaggtaaa
      121 tatcgggtag agaacaatac tgaaatgaaa ttaggtgaag ttcattattc taaaactggt
      181 tttcatgtag ttccgtatat taaaaaggag tgattaaaaa tgaatttaca gtcatacata
      241 ggcaaattag tcaaattaac gcttactaat aataaaatat taattggaaa agtgatagac
      301 tttgatgata aaattgataa ttttgatggt tataattcga tagaaattga tacaggtaga
      361 attacatatg atatatcaga aaataaaatt aaagacatac aaataattac aaaatagcat
      421 cctttctaca cagataaaga gaaagcggtg ctatttttat acactttttt aacaactaag
      481 gatacaagac acataccttc aacaatatcc tcatctaaac ctatcgattt cctaagctca
      541 attttaaaca caaagtttat ttgtaaagat gcacacctat aaaaatctta atcatcgaaa
      601 agtcacaaac atctccatat taaaaactta aatagtgcat tccatttaaa ctaaacatcg
      661 atatttctac caatatagac ttagtaattc gattgttaat acattacttt cataaaataa
      721 ctacaaccat cataatactt ctcaaatctc gtcaaattaa agtaaataca gcacaaatta
      781 acaattttaa atatataaaa taaaacaaga tactcaataa tctgtagtac cttgttttgg
      841 aataatgtaa ttttatatca tcaacggcta caaatgagca gtttcatata taaataaaca
      901 caactactca actgatcttg attaattaat aaatgatttg ccagatgaca tataacagca
      961 ttattagatt aattctaata gtttatttaa attttcttcg gttgtcgccc aactggttgc
     1021 gaatctaaca acacgatgtt gatcatcgta tttttcccaa acagcaaatt taactttttg
     1081 ttctaactct gctattttct cgttacttaa aataaaaaat tgttgattgg ttggagaatc
     1141 aaagtaaaga cgatagcctt tatttataaa cccgtctttc atcttatttg ccatttcgat
     1201 agcatgtctg cttatattaa aatataaatt atccgtaaat aattctaaaa attgtatgcc
     1261 tgttaaccgt ccttttgcta aaagtgcacc gtgatgtttg attcgagtgg taaattgttt
     1321 cggttcatta ttcttcgtaa aaacaattgc ttctccgcat aatgcaccta tcttcgtacc
     1381 acctatataa aatacatcac aatatttagc gatgtcttta atagtcatat ctgattgatc
     1441 actcatcaat ccatacccta atcgtgcacc atccataaat aatggaagct gatattgctt
     1501 acatactttg cataactctt ccaattctga tttagagtat aatgtgccat attcagtagg
     1561 atgagaaata tataccattc ctgggaatac catatggtcc tttttaaaat cacttttaaa
     1621 tgtctccatg taagtttcaa catctgaagc actaactttt ccttccttag agggtatagt
     1681 aattacttta tgtccactat attcaattgc accgccctca tgcacagcaa catgaccagt
     1741 gtctgctgaa atgacccctt cgtaactttc taacattgaa ttaataacaa cctgattggt
     1801 ttgtgttcca cctactaaaa aacgaattgt agcatttgga cagtcaattg tatctttaat
     1861 cttttcaatt gcctgagctg tgaattgatc aaagccatat cccgaagctt gtacaagatt
     1921 tgtatctact aatcgtttta atactttttc atgagcacct tctaaataat cattttcgaa
     1981 tgaaatcact acatttcccc ctaaaactaa tatcaacatt ttaataagat aaaccaattt
     2041 caaaactagt tcgatattta aaatgtatta tgaatggtta aagtttgtat cgcattatcg
     2101 cgaagttgaa taaatatatt atacatcaaa caatacatta tgaaaattaa ttaatttttt
     2161 accacaatta ctttgcgtgc cgaaacacat atattaatga ttgaatttta ctgttaaaag
     2221 ttctatgatt taggaaatag agaaaagagg ggatttaaat ggaacaaatt aaacttaaaa
     2281 cttttacatc tgagacttta gaattattag aatcaaatat taacgaattt ttaggttctg
     2341 aagaagctgc aaatttgaaa ttagtaaata ttacaataaa agaaattgag gaaagaacat
     2401 ttccaaataa tgaagaagag tttaatgcaa tattaactct atctgtaaat aaataatctg
     2461 gaacgaaagg tacaaaagtt ttttgtgcct ttctttatag gagaaataaa ctatgcaatt
     2521 ttcattttca aacgatttag gaacgttatt tactattatt ttagccattg gattcatcat
     2581 taatttagta ttagctttta ttattatctt tttagaaaga aataggcgta cagcgagttc
     2641 aacttgggca tggctatttg tactttttgt cttacccttg attggtttta ttctttactt
     2701 gttttttggt agaaccgttt cggcacgcaa attgaataaa aacaatggta acgtgttaac
     2761 ggatttcgat ggacttttga aacaacaaat agaaagcttt gataaaggta attatggtac
     2821 tgataacaaa caagttcaaa aacatcatga tttagtacgt atgcttttga tggatcaaga
     2881 tggtttttta actgaaaata ataaagttga tcatttcatt gacggtaatg atttatacga
     2941 tcaagtttta aaagatatta aaaatgcaaa agaatatatc catttagaat actatacttt
     3001 cgctttagat ggtttaggta aaagaatttt acatgcttta gaagaaaaat tgaaacaagg
     3061 tttagaagta aaaatattat atgatgatgt tggttctaaa aatgttaaga tggcaaattt
     3121 tgatcacttt aaatcattag gtggcgaagt tgaagcattt tttgcgtcta aactaccatt
     3181 attaaatttc cgtatgaata acagaaatca tagaaaaatc attattattg atggtcaatt
     3241 aggttatgtc ggaggattta acattggaga tgaatacctt ggtttaggta aactaggata
     3301 ttggagagat acacatttac gaattcaagg agatgcagtt gatgcattac aattgcgctt
     3361 tattttagac tggaattcgc aagcacatcg acctcaattt gaatatgatg ttaagtattt
     3421 ccctaaaaag aacggaccat tgggtaattc accaattcaa atagctgcaa gtggcccagc
     3481 tagtgactgg catcaaattg aatatggtta tacaaaaatg attatgagcg ctaagaagtc
     3541 ggtatatttg caatcgcctt atttcattcc ggataattca tatataaatg ccattaaaat
     3601 tgcagctaaa tcaggtgtag atgtacattt aatgattcca tgtaagccag atcatccttt
     3661 agtttattgg gcgacatttt caaatgcctc tgacttatta tcaagtggtg ttaaaattta
     3721 tacgtatgaa aatggattta tacattctaa aatgtgctta attgatgatg aaatcgtatc
     3781 agtgggcaca gcaaatatgg actttagaag ttttgaatta aattttgaag taaatgcctt
     3841 tgtatatgat gaaaatcttg ctaaagattt aagggtggct tatgaacatg atattacaaa
     3901 atcaaaacaa cttactgaag aatcatatgc caatagaccg ctgtctgtta aattcaaaga
     3961 atcgttagct aaattagttt cgccaatttt ataatttatt tgtaaggagt ctcgattata
     4021 gaggctcttt ttattttatc caaattaaat aacgataaat atgttatgta aacctaatga
     4081 tgacatttgt catttcaaat ctatgacaaa gtatcctatt ttaaatcgaa taaatttact
     4141 aatcttaaat taataaaata ttaagaggtg gttcatttga ttcaaatatc taatattaat
     4201 aaatcattta agaaaaatcg tgttttaaaa aacatttcat ttgatattga aaaaggtaca
     4261 tgtaccgcat taatcggaaa aaatggtgct ggaaaatcaa cattagttga tattttaagc
     4321 aataaaataa ttgctgatga tggcgtgatt ttggataaag acaaattatt gcaaagtgaa
     4381 aatcgcagta taatgtttca gaaaacgatg tttccagatc aattgaaggt aattggaatt
     4441 atcaaattat atcaatcatt ttatgaaaat ccattaacat tggacgtaat aatagaacta
     4501 acaaagttta attctaatca attgaatcaa tttgcaaata aattatctgg aggtcaacaa
     4561 cgattacttg attttgtaat atctttaata ggacaaccac agcttatttt gttagatgaa
     4621 ccaacgtcaa atatggatat tgaaatgaga gaatattttt ggtcaataat agctaagtta
     4681 aaagaagaaa atcggacgat tctttataca tcgcactata tagaagaagt agagcgcatg
     4741 tccgacaaaa ttattttgat agaaaatgga gaaatcaaac ttaatgattc aacgtcacat
     4801 attagaacca atcagcaatc tcagattacg ttatccgatg aatataaaag aaagttaaaa
     4861 ccagataaag atgatttagt tattcaaaaa aatcataatg gcactatcaa aattattact
     4921 tcaaatgtaa atgatacgat tttatatctt caacaacttc atattaattt ggatgatatt
     4981 gaaatacaaa aagtctcaat tgttgattca tacttcaaca ataaaaagca aaggggatct
     5041 aattatgata ctaagttact tgaaaatcga atttaaagtt atactgcgta aaaaaacaac
     5101 attaatatta tctattttat ttcctgttat tttctatata ttatttactt cgatattgga
     5161 tttgccggaa gatgttaaac ctaaatttta taaagagtat atgtatagta tgacggttta
     5221 tagtttgtta agttttagct tactaacttt tccattagat attattaatg aaaaacaaaa
     5281 tggatggcgc caaagattaa tggtaacacc atttactttt actagttatt atatttcaaa
     5341 agtagtgaaa actatgctgc aatttgcaat agcgatatta gtgattttta tggttgggca
     5401 tttttataaa ggtgttgaaa tgagtgcagt tcaatggtta gagtcaggaa tatttttatg
     5461 gttaggtgcg tctctattaa taacttttgg catattattt tctttgttaa atgatattca
     5521 aaaaacaagt gctttagcca atatcgtaac aattggatta gcagtattag gtggattgtg
     5581 gtttccgata aacacatttc cgaattggct tcaacatgtt gctcatgttt taccgagcta
     5641 tcatttgcgt aaactaggtg tagatattgc ttcaaatcat catatcaatt taatatcatt
     5701 tgttataata ctcttgtatg ctttagggag tattatagca gtatattgta ttagtcattt
     5761 taaaagggcg gaataaaata tgaaattttt aaaagatact tcaattgctg aaatatcgtc
     5821 tatactttat ctaatttttc ctattgccgg tatatttttt aatgaagtat atggtcccac
     5881 atggttgtat attatatcag tcattgtctt ttcgttatcg tatcttatat tagttatagt
     5941 aaataataga cttaatacat taatgtttta cattttgttg attattcatt attttattat
     6001 ttgttatttt gttttcagtg tacatccaat gctaagtttg tttttctttt atagtgcttt
     6061 tgccattcca tttactttta aaaataatgt taaaaaaatg gcaactaatc ttttcatatt
     6121 aacgatgatt atatgtttta caattactta cttagtacat aacgactatt ttgttgcaat
     6181 gacgatttat tatgttgtta ttttgttaat cgtgtttgat aatttaaaaa aagtgaaaga
     6241 tcgtgaatat aaaaaagaaa tagaagaaaa aaatcggtat attaatacgt taataactga
     6301 acaagagcgt catagaatag gtcaagattt acatgatacg cttggtcatg tgtttgcaag
     6361 tttatcatta aaatcagaat tagcttataa aattattgat tctgatactg aaaatgcaaa
     6421 agctgaattg ttagcgatta ataagttgtc gcgggaatca ttgaacaaag tacgcgaaat
     6481 catcgatgat ataagattac catcatttat tgaagaaatt gagagtatct gtaaaatttt
     6541 aaaagatgct gacattcagt ttacgtttga aaataaagaa ctggcacaat tattgagtcc
     6601 tacaaaacaa tccattttag taatgattgc tcgtgaagcg ataaacaatg tgattaagca
     6661 tgcaaatgct tcaaaagttc atggtcaatt acgagctatg aatgaacata aattacagtt
     6721 cattatacag gataatggag aaggtattga cagcggctgt gaaataaaga gtatttcaca
     6781 acgagtaaaa catttaaatg gaacattaga agttgaatcg aataaaggta ctaaattaat
     6841 catcgaaatg cctacagggg gaatagcatg atttctttaa ttattgcaga agatcaaaat
     6901 atgttacgac aggcaatgat tcaattaatt aaactacatg gtgattttga aattttagcc
     6961 gatgtagata atggtctcga tgcaataaaa attattgagg cacaccatcc tgatgtagtt
     7021 attttagata ttgaaatgtc aggcatgact ggacttgaag ttttatcaga aattagaaag
     7081 aagcatttga atattaaagt gattattgta acaactttta aaaggccggg gtattttgaa
     7141 aaagctgttg caaatgatgt ggatgcatat gttttaaaag aacgttctat agaagaatta
     7201 gtggaaacaa tttataaagt gtataacggt aagaaagaat atagtgcttc tcttatgact
     7261 tcatttttca cagataaaaa cccattaacg ccaaaagaac aaattgtatt aagggaaatt
     7321 ggcaatggtt taagtagtaa agaaataagt gaaaaattat ttttgacaga tggaacagtt
     7381 agaaattata catctgttag aattgataaa ttatttgcag ataatcgttt tgatgcttgg
     7441 aaaaaggcaa atgaaaaagg ctggatctaa atacaaacaa aaaagtattg agtgttttga
     7501 tttaagagac tttgaaagtg agtcaataat caaaatctta ctcaatactt ttattttggt
     7561 actcgcaagt tagatttgtt aactaatttt ttcgtttatt ataaaacgaa acaatcaggt
     7621 aaacgataaa gcctacaaag atacccaata aaatagatag tactgccgtc actattaatg
     7681 gtaatttaaa aaatatttgt aggaaaatac caatgataat tgcgataatt actgcaatta
     7741 atgtgactgt attttcattt gaaatgttca ttcattttca ctccttaaca ataacattat
     7801 atcatgctat agctttccaa aatattgaaa tatgtagata tggctattga cgatatttct
     7861 taacttttat atgattaatc ggaatgaaaa aagagaagta ggtggcaaaa tgaagtcaaa
     7921 taaatcgctt gctatgattg tggtagccat cattattgta ggtgtattag catttcaatt
     7981 tatgaatcat aagggtcctt ttaaaaaggg aacaaatcat gaaactgtac aagatttaaa
     8041 tggtaaagat aaagtacatg ttcaaagagt tgtggatggt gatacattta ttgcaaatca
     8101 aaatggtaaa gaaattaaag ttaggcttat aggggttgat acgccagaaa cggtgaaacc
     8161 gaatacgcct gtacaaccat ttggcaaaga agcatcaaat tatagtaaga agacattaac
     8221 aaatcaagat gtttatttag aatatgataa agaaaaacaa gatcgctatg gtagaacatt
     8281 ggcgtatgta tggataagta aagatcgtat gtacaataag gaattagtgg aaaagggact
     8341 tgctagagaa aagtattttt caccaaatgg caaatataga aatgtattta tagaagcaca
     8401 aaataaagct aaacaacaga aattaaatat ttggagtaaa taattaaata ggtgattata
     8461 tatattaaag taaaatatac ttgaagcatc gatttattat taccataaat ttatatatag
     8521 aaataattta aaggcataat aaccatagtg cgtatcgagc gtaatggatt attatgcctt
     8581 tgattttact tgaatatagc tgtgatttga ttaacctgtt ctttatttaa agggtgttta
     8641 gatagctcta tggcttcgct aataatacgt cgtaacgaag attttgttga aactgtatca
     8701 acatatttat ctctttcttc agagccttga atgatattaa ctgtaccatc actaaagtaa
     8761 ataacaccgg tactttggac tttaaagtta aagttttgtt caatatgatc ctttaatgct
     8821 ttagcattat ttgctgctaa ttgatacgga tcataatcat aaaaatcata aataacacga
     8881 tttggttgaa cagtttctgt aaaagtatag atagactttc ttgatgaatt aaactgatca
     8941 tgatattgct gactaatgta atgaccgaca actttttcaa tattactatt gctcatttct
     9001 gtatcatgct catcaggtac atcaaagtga taaaatgttt tttcacccca actttttaca
     9061 tcaacgttta ttaaaccgat atctgaaaca atgataaaat caaatgaacg tgcatactcg
     9121 aaaaacggat gtttagtagc taaattgctt gtaacgataa tatcgtaata ttttaattta
     9181 ccattttcga gatagctgtc taaaatcttg cgtaattctc ttttagcatt tttgtatgca
     9241 tggtggccaa catcattatt agaactcagc atttgacttc ttaattcagc attttctgca
     9301 cttaatgctt tgttcttttt aataagttgc tttcttgcat aaacttcggt atctatttta
     9361 ctattactat acctttgatt taaaactaat ataccaatta atgctacaat gataatgata
     9421 agtacaacat aaaaagacat tttttcacca atcctttttg acttctttaa ctttgtatac
     9481 aataataatt aataaagatt aattgttatt caatttccca catttttatt agttgatttt
     9541 agttcatcat tgttataatc aaattataaa ctgacagata ttgatgttca atgaatatga
     9601 cgtgaaagat tcgtgaattc aagtttatgt cgaatttatg ttataacggt catttaaatt
     9661 acagaattag gtcactcata gtattttgaa gattgaattc attaatttta aaatgtataa
     9721 tgatatttgt gaaagcgctt gcttaggagg tgtatttgag agtgaatgaa atgaatgcta
     9781 aagaacaatt agtggacaat ttaatgaaaa catcatcgca attatttaaa tttcacggtg
     9841 aagttgccat gcagcttttc ttaaatgatg aattaaaatt accttctatt gttgaaatat
     9901 gcgtggaacg taagcgttta agtgatattg tgaaagttat tccgcaatca tatgcgttac
     9961 tatacataga taagcaagat caagcaatag ctaaagaaga tttatcactt tcaaaaattg
    10021 caaaagttta tgtgcaatat gataatacaa caataatgag tattttcgtt tatgatgttg
    10081 taaacgatga atggattttt agattggatc cgaatatacg tatacctaag agtaacattt
    10141 acttccatag tctaaattgg gatgtggatt atattaaacc agagattgtt ctaatgtatg
    10201 atctaatgca acaccatcag tatcatcatt attccaatta taaacgcgtc atagatgcat
    10261 taagctacta tcaatttttt attttaaaat ttgtagtagg tgagcaacgt attaaggatg
    10321 caatccagag aacaataaat aattaagaaa aagcaattca taacgcagtt gaatacatgt
    10381 gttacgaatt gcttttatat tagtttttat cacacaagtt ttttaatgca accccgtgat
    10441 agcaaaactc atatgtagat aatacagctt tttcagcatc atctacatga attccaaaca
    10501 tcatcgaaat ttccgaagca ccttggttaa tcatttttaa gttaattttt gattcagcta
    10561 aggcatgtgt aattttattt gcagtaccaa tgactttatt cataccttcg ccgacaatca
    10621 ttaaaattgc taaatcatgc tcaatactta gctcatcaac atcacatttt tgacgaattt
    10681 catttaatac ttttgtttct ttattttgaa tttgttttga acgcataacg atactgatag
    10741 tatcaatacc tgaaggcata tgatcaaatg aaatattatt atcctctaag acacctaata
    10801 tctttctagt aaagccgact tgtctattca ttaaatactt tttgatatta ataacagtaa
    10861 aatctttatc acaacttata ccgctaatca catttttcgc atttatttct ctatcatgca
    10921 ctataaatgt acctttatct tgagggcggt tcgtattttt aataactaca gggatgcgat
    10981 ctttataaag tggttgtaag gcttcatcat ggaaaacact aaaaccagca taagataatt
    11041 cacgcatttc tcgataagtg atttcttcga ttaattcagg atctttgatg atatttggat
    11101 tagctttata aataccagac acatcggtga aattttcata aattgtagct ctaacaccac
    11161 ttgatatgat ggcgccagtt atatctgatc cgccacgtgg aaatgtaact atatatcctt
    11221 catgagatac gccaaaaaat cctgggataa ttagtttttc atcataatct cttaattttt
    11281 taatttcaga gtaagcacta tctaatattt gtgcttcttg tgggacgtca gtaacaaaaa
    11341 tacccgcttc cttcggtgat atatattttg ttggtatacc ttgactatta ttatataaag
    11401 ctatcaattg cgcattaaaa tcttcaccac aagaaagtaa tgcatctaat agtctcttcg
    11461 gttcattttt taattgatta atataatgtt ccaaagtcac atctatcgtc cgtaaaatac
    11521 tttcatccat ttgcaattct tttacaatat catcataacg ctgaataatt tctctttttt
    11581 tatcatgata atcaagatga ttaatgacct tttcatataa tctgattaac aaatcagttg
    11641 ttttaatatc attatcatgt cttttacctg gagcagaaac gataacaatc tttcgctctg
    11701 gatcagaatt aacaatattt aaaacctttt taatttgagt agcattggag acggagctac
    11761 caccgaattt ggaaactttc atagtgcatg ccaacctttc taaaaaatca gaaaattgta
    11821 ttcagttttg taagtaagaa tgatattatg tatttatatt ttgaatatta cgaactttac
    11881 aaaaataaag aataattcta tactatacta tcatctagtg tttttcaata atacatttgt
    11941 ttttctcaaa agcacaaacg gaggtacata aaatgaaaaa attaaatata gcattattag
    12001 gattaggtac tgtcggatct ggtgttgtta aaatcatcga agagaaccga cagcaaattc
    12061 aagatacatt aaataaagat attgtcataa agcatattct tgttcgagat aaatctaaaa
    12121 agagaccgct aaatattagt caatatcatt taactgaaga tgttaatgaa attttaaatg
    12181 atgattcatt agatattatc gttgaagtca tgggaggaat tgaaccaact gtagattggt
    12241 taagaacagc acttaaaaat aaaaaacatg ttattaccgc aaataaagat ttattagcag
    12301 tacatcttaa acttttagaa gatttagcag aagaaaatgg tgtagcttta aagtttgaag
    12361 cgagtgtagc aggtggtatt ccgatcgtaa atgccataaa taatggtttg aatgcgaata
    12421 atatttcaaa atttatggga attttaaatg gtacctctaa ttttatttta tcaaaaatga
    12481 ctaaagagca aacgacattt gaggaagcac ttgatgaagc gaaaaggctt ggttttgctg
    12541 aagcggatcc aactgatgat gtagaagggg tagatgcagc gcgtaaagtt gtcattacat
    12601 catatttatc atttaaccaa gtcattaaat taaacgacgt taaacgaaga ggaattagtg
    12661 gtgtaacttt aactgatatt aatgtagccg atcaactggg gtataaaatt aaattgattg
    12721 gtaagggaat atatgaaaat ggcaaagtta atgcatcagt agaaccaacg ttaattgata
    12781 aaaagcatca attagcagct gtagaggatg aatataacgc gatttatgtc attggtgatg
    12841 ccgttggtga cacgatgttt tatggaaaag gagcaggcag tttagcaaca ggtagtgccg
    12901 ttgtaagtga tttattgaat gtagcattat tctttgaatc agatttacac acattgccac
    12961 cacattttga attaaagaca gataaaacaa gggaaatgat ggattctgat gcagaaatta
    13021 atattaaaga aaaatccaat ttctttgtag tagtgaatca tgtcaaaggt tcaattgaaa
    13081 attttgaaaa tgagttaaag gcaatattac catttcaccg atcattaaga gttgcaaatt
    13141 acgataatca agcatatgcc gctgttatag ttggattgga atcatcaccg gaagaattaa
    13201 tcactaagca tggatacgaa gttgacaaag tatacccagt agaaggagtt taattataat
    13261 gagaagatgg caaggattag tagaagagtt taaagcacat ttaccagtaa atgaaaatac
    13321 accaaaatta actttgaatg agggaaatac accactcatt cattgtgaaa atatgtctga
    13381 aatactaggt atagatttat atgtgaagta tgaaggtgcc aatccgacag gttcatttaa
    13441 agatcgcggt atggtaatgg ctgtgacaaa agcaaaagag caaggtaaga aaattgtaat
    13501 atgcgcttcg actggaaata catcagcgtc tgcagcagca tatgcagcga gagcaggttt
    13561 aaaagctatc gtcgtaatac cagaaggtaa aattgcatta ggtaaattgt cgcaagcagt
    13621 aatgtatggt gcagaaatcg tttctattga aggaaacttt gatgaagctt tagaaattgt
    13681 aaaagaaatt gcaaaaagtg gcgaaatcga gcttgtaaac tctgtcaatc cacttagaat
    13741 cgaagggcaa aagacaggct catttgaaat tgtacaacaa ttagacggtg aagcacctga
    13801 tattttagcg attcctgtag gtaatgcagg taatattact gcatattgga aaggctttaa
    13861 agaatatcat gaagctaaag gatcacaatt gccgaaaatg tttggcttcc aagctgaagg
    13921 tgcatcacca attgttcaaa ataaagtcat taaaaatcct gaaacgattg caactgctat
    13981 tcgaattggt aatcctgcta gttgggataa agcgactaat gctcttaaag aatcaaatgg
    14041 attaatagat agtgttactg atgatgaaat tttagaagca tatcagttaa tgacaactaa
    14101 agaaggtgtc tttagtgaac cagcaagtaa tgcttctatt gcaggtttaa ttaaattgca
    14161 cagacaaggt aaattacctc aaggtaaaaa agtagttgcg attttaactg gtaatgggtt
    14221 aaaagatcca gatactgcta tttcactatt agataatccg ataaagccat tgccaaatga
    14281 taaagatagc attatcgatt atattaaagg agctttataa catgtcgaat gttttggagt
    14341 taacaattcc tgcatcaaca gccaaccttg gagttggctt tgattctata ggtatggctt
    14401 tagataaatt tttgcatctg tctgtaaaag aaacatcagg gacaaaatgg gaatatattt
    14461 tccatgatga tgcatcgaag caattaccta ctgacgaaac aaactttatt tatcatgtgg
    14521 cacaacaagt tgctgctaaa tatagtgttg acttgcctaa tttatgtatc gaaatgagaa
    14581 gtgatattcc gttggcaaga gggttaggtt cgtcagcttc tgctttagta ggagctatat
    14641 atatcgcaaa ttattttggt gatatccaac tatctaaaca cgaggtatta caattagcga
    14701 ctgaaatcga aggacatcct gataatgttg cgccgaccat ttatggtggt ttaatcgctg
    14761 gatattataa tgatgtcacg aaagaaacgt cagttgcaca tatcgacata ccagacgtgg
    14821 atgtgattgt aacgatacca acttatgaac taaaaacaga agcatcaaga cgtgctttac
    14881 cacaaaaatt aacacatagt gaagccgtta aaagtagtgc aattagtaat acaatgattt
    14941 gtgcattagc acagcacaat tatgaattag caggtaaact catgcaacaa gatggctttc
    15001 atgaaccgta tcgtcagcat ttaattgctg aatttgatga agtgaaaaca attgctagtc
    15061 aacataatgc ctatgcaact gtaattagtg gtgctggacc aactatttta atatttagtc
    15121 gcaaagaaaa tagtggcgaa ttagttcgcg ctttaaatag aaatgtagtt acatgtcatt
    15181 ctgaattagt ggatattaat gttagtggtg ttaaagaacg aattgtatac caatagacac
    15241 tttatattgt aaaatagtat tgaatgtgaa atagagagga gatttaatgc gatatgacaa
    15301 attataaagt tgtcgtttta gacatggatg acacattgct aaattcagat aatgtgatat
    15361 cagaagaaac tgcaaattat ttaacagcaa ttcaagatga aggttattat gttgttttag
    15421 catctggtag acctactgaa ggtatgattc caactgctag agatttaaaa ttacctgaac
    15481 atcatagcta tattattagt tataacggca gtaaaacgat taacatgact aatgaagaag
    15541 tagaagtaag taaatcgatt ggtaagcagg atttcgatga aattgtagat tattgtcgag
    15601 atagaggctt tttcgttctt acatatcatg atggtcaaat tatttacgac agcgaacatg
    15661 aatatatgaa tattgaagca gaattaactg gtttaccgat gaaacgtgtt gatgacatta
    15721 aagcgtatat tcaagatgat gttcctaagg tcatgggtgt agattatgta gcgaatatta
    15781 cagaagctag aattgatttg aatggtgtgt tcaatgataa tgtagatgct acgacaagta
    15841 agccattctt cttagaattt atggctaaag atgtttcaaa aggtaatgca attaaagcgt
    15901 tatgtcacaa attgggatat tcggtggatc aagtcattgc ttttggtgat agtatgaatg
    15961 ataaatcaat gtttgaagtc gcaggtctag ctattgctat ggggaatgca tcagatgaac
    16021 ttaagcaata tgcagatgaa attacgttgg accataatga aaatggtatt ccacatgcgc
    16081 tcaaaaaatt gttataaatt ttaaaataag cctaagcaca ggatatttga ataagatatc
    16141 ttgtgtcaag gctttttatt tatgtgaaaa tgcgttagct ataatatggc gcatttgaaa
    16201 tacatattat agattagtaa tgatatctaa agaatagagt agatgcatat tttttaagta
    16261 aatcaagtat taattagtca cggaaggtag ataaataaat ttgaatcatt ctgagtaata
    16321 acgaatgtat taaaaaatgc agtgacaatc taacattaat tgttaaaatc actgcaagca
    16381 tcttgatttt atttagctgt ttgtgtccag tcatgcttcc ctctgaagcg ttttacatga
    16441 gcatatattt ggtcagcagt ataatctaaa tttgtataaa ctgtaatact gaacgctgat
    16501 tggttttcag caaatgattc ttcggaaaat aattccttta agtgcgtaaa taaatcattc
    16561 atttgtttat ggtttaaacc gggatattcc cttaaagttc tctttaataa ttgtccgttc
    16621 ttttcttcta atgcttgaac tacaataaca aatctctcat cttgctttag tacatcatca
    16681 aataaatttg tttgtccaat cattgaattt cacccctgaa aagttattgt tatctatatt
    16741 atacacgata tgcaaaatat ctaaaataaa gaatggtaac atggtcataa taaaatataa
    16801 acagaatact agttaataca ctttatacta gaaaaagata ttaaagtata tctactttac
    16861 accattaaaa aagcggcaaa tgctatagat aatctaaagc atctgccgtg tgggaattta
    16921 tttatttttg attgtcataa tcgtgtggtt ttaaattaat tgtttctagc tttacaaatt
    16981 ttgttttgtg aatgatttta tgaataaagt aaatcaacgc tagaatgatt aaaggtaaaa
    17041 agtttttaaa agcatttaac cattgatctt ttaaaatata ttcaactgaa ccaccaaata
    17101 gcaagaataa tagcgtagcg atgacaatga ttggtcctaa tggataaaaa ggtgctttat
    17161 atggtaggac cttattagga tcttgacctt gttttttaat agcttgtcgc aatcgtattt
    17221 gtgaccaaat gcttgatccc caaacaacta taatcattga accaataatt tcaagtaaat
    17281 taaaaacggc atttgaatta aagtttgcat aaataataac aataacaacg actgcatatg
    17341 tagttaataa tgctcttaat ggtaattttg ttgtcttgtt taatttactt aaaaatttag
    17401 gtgctttttt gtctgaactt aaggaataca acattctgcc tgttgtataa acacctgaat
    17461 ttgcagcgga taatagtgaa gttaaaataa ccgcattgat tactgatgct gcaaaggcta
    17521 tgcctactct atcgaataca attgtaaatg ggctttgact tattgaacta cttgctctta
    17581 ataatgatgg atctgtgtac ggaataattg caccaatgac tgcaattgat aatacataga
    17641 ataaaagaat acgccaaaac acttgtttaa ttgctttagg catagacttt ttaggatcat
    17701 ctgattcgcc agcagttact gctactactt cagtaccacc aactgagaat ccagcgacta
    17761 ataatacgcc taggaaacca gagataccac caacaaacgg tgcttggcct tttgtatagt
    17821 tttcaaatcc atatgcatga ccacctaaga taccgaaaat cattaaaaag ccaaaaataa
    17881 cgaatacgat gattgttaac actttaatta atgataacca aaactcagtt tctccaaatg
    17941 attttacaga aaaaatgttt aataatagta aaattgtaat aaagattaag ctccaagtaa
    18001 tggggtggaa aaatttaaat gtgtcccaga aataaagcac atttgacgct actatgacat
    18061 caacacttgt aactaatgac cacaatgccc aatacaacca tcccatggta aagcctagag
    18121 atgagtcaat aaagcgtgtt gaataagagc tgaatgaacc tgatactgga taaaatgttg
    18181 ccaactctcc aattgatgcc attaagaaat atagcatgac accaataaca agataagcga
    18241 gtatagcgcc tccaggacca gcttgagaaa tgatattacc agtagctaca aatagaccag
    18301 tcccaattgc accacctata gcaatcatgg aaatgtgtct tgagttaaga ctacggttca
    18361 ttttattatc ttccatattt agtctcccat ctatttaaat atacccatta ttgtaagctt
    18421 tttaagtgta ctattcaata actatttagc actgtatagc ggaaaaatta aaattttctg
    18481 attttttaat catcttgagc atgtttaatt gtaattctga tggggttaaa ttataatatg
    18541 tattaaatta taattattat aaattgtgga aggatgacta tgtcacgaca agataaaaag
    18601 ttaactggtg tttttgggca tccagtatca gatcgagaaa atagtatgac agcagggcct
    18661 aggggacctc ttttaatgca agatatttac ttcttagagc aaatgtctca atttgataga
    18721 gaagtaatac ctgagcgtcg tatgcatgcc aaaggttctg gtgcatttgg gacatttact
    18781 gtaactaaag atataacaaa atatacgaat gctaaaatat tctctgaaat aggtaagcaa
    18841 accgaaatgt ttgcccgttt ctctactgta gcaggagaac gtggtgctgc tgatgcggag
    18901 cgtgacattc gaggatttgc gttaaagttc tacactgaag aaggaaactg ggatttagta
    18961 gggaataaca caccagtatt cttctttaga gatccaaagt tatttgttag tttaaatcgt
    19021 gcggtgaaac gagatcctag aacaaatatg agagatgcac aaaataactg ggatttctgg
    19081 acggggcttc cagaagcatt gcaccaagta acgatcttaa tgtcagatag agggattcct
    19141 aaagatttgc gtcacatgca tgggttcggt tcacacacat actctatgta taatgattct
    19201 ggtgaacgtg tttgggttaa attccatttt agaacgcaac aaggtattga aaacttaact
    19261 gatgaagtag ctgctgaaat tatagcaaca gatcgcgatt catctcaacg cgatttattc
    19321 gaagccattg aaaaaggtga ttatccaaaa tggacaatgt atattcaagt aatgactgag
    19381 gaacaagcta aaaaccataa agataatcca tttgatttaa caaaagtatg gtatcacgat
    19441 gagtatcctc taattgaagt tggagagttt gaattaaata gaaatccaga taattacttt
    19501 atggatgttg aacaagctgc gtttgcacca actaatatta ttccaggatt agatttttct
    19561 ccagacaaaa tgctgcaagg gcgtttattc tcatatggcg atgcgcaaag atatcgatta
    19621 ggagttaatc attggcagat tcctgtaaac caacctaaag gtgttggtat tgaaaatatt
    19681 tgtcctttta gtagagatgg tcaaatgcgc gtagttgaca ataaccaagg tggaggaaca
    19741 cattattatc caaataacca tggtaaattt gattctcaac ctgaatataa aaagccacca
    19801 ttcccaactg atggatacgg ctatgaatat aatcaacgtc aagatgatga taattatttt
    19861 gaacaaccag gtaaattgtt tagattacaa tcagaggacg ctaaagaaag aatttttaca
    19921 aatacagcaa atgcaatgga aggcgtaacg gatgatgtta aacgacgtca tattcgtcat
    19981 tgttacaaag ctgacccaga atatggtaaa ggtgttgcaa aagcattagg tattgatata
    20041 aattctattg atcttgaaac tgaaaatgat gaaacatacg aaaactttga aaaataaatt
    20101 tgatatgtag tttctatatt gcgtagttga gcagtttatg atatcataat aaatcgtaaa
    20161 gattcctaac aagagagggt gtttaacatg cgcgtaaacg taacattagc atgcacagaa
    20221 tgtggcgatc gtaactatat cactactaaa aataaacgta ataatcctga gcgtattgaa
    20281 atgaaaaaat attgcccaag attaaacaaa tatacgttac atcgtgaaac taagtaattc
    20341 ttatcattca aatacgacga tttgaaaata aagcgggctt acctattata tgggggagct
    20401 cgctttttta tgaaattttt gtgaagagtg attaatgaat tgagtttcat cgatagaaca
    20461 atatatgttt gtattagttg ttactttatt aaaatttgag aatattgata gaaggaaata
    20521 gattactaat tttataaagt cactttgtta gcgaatgctt gaaagagtaa ttaatatagt
    20581 agaatttaaa atttcaatgc gggatttaat aagtaagaag taattgtgga tatgttttat
    20641 aaatgttcga caatacactt tgatgttaaa tttgatggtt taaaaaatga tttaacaaag
    20701 aaatgaaact ttactgttga attatgtgag gattgtgtta ttatataaat cgtaataatt
    20761 acgatttgat aaaaagtgag gtaactatat atggctaaga aatctaaaat agcaaaagag
    20821 agaaaaagag aagagttagt aaataaatat tacgaattac gtaaagagtt aaaagcaaaa
    20881 ggtgattacg aaacgttaag aaaattacca agagattcat cacctacacg tttaactaga
    20941 agatgtaaag taactggaag acctagaggt gtattacgta aatttgaaat gtctcgtatt
    21001 gcgtttagag aacatgcgca caaaggacaa attccaggtg ttaaaaaatc aagttggtaa
    21061 aatcaaattt cgtactttag cccatttaca atatcaataa aacaatgtac agtatatacg
    21121 aatgctataa actgaatgtt ttctcatatt aataaggaaa cattcggttt ttaatttgca
    21181 tttaaaaata attagttttt aaaggggcta tttaaagtga aaatatttga ttacgaagat
    21241 attcaattaa tacctaataa atgcatagtt gaaagtaggt ctgaatgtga tacaactatc
    21301 caatttggtc cgaaaaaatt caagctacct gtagttcctg caaatatgca aacagttatg
    21361 aatgagaaat tagcgaaatg gtttgctgaa aatgattact tttatatcat gcatcgtttt
    21421 gatgaagaag caagaatacc ttttataaaa catatgcaaa attcaggctt atttgcatct
    21481 atttcagttg gtgtaaagaa agcggaattt gattttatag aaaaattagc tcaagaaaag
    21541 ttaatccccg aatatattac aattgatatt gcgcatggtc actcagattc agtgataaac
    21601 atgattaaac atattaaaac ccatatacct gatagttttg ttattgctgg taatgttggt
    21661 acgccagaag gtgttagaga attagaaaat gctggtgctg atgctaccaa agtcggtata
    21721 ggtcctggta gagtttgtat tacaaaaatt aaaacaggtt ttggtactgg tggttggcag
    21781 ttagcggcat taaacatatg tagtaaagca gctcgtaaac ctttgattgc cgatggtggt
    21841 ataagaacgc atggcgatat cgctaagtca attagatttg gtgcatcaat ggtcatgatt
    21901 ggttcattat ttgcggcaca cgaagaatca cctggtgaaa ctgtagaact tgatggtaaa
    21961 cagtataaag aatattttgg tagtgcatct gaatttcaaa aaggcgaaca taaaaatg
//
LOCUS       DAMZPP010000032        21691 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMZPP010000032 DAMZPP010000000
VERSION     DAMZPP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21691
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.032"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(126..332)
                     /locus_tag="POH73_000919"
     CDS             complement(126..332)
                     /locus_tag="POH73_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886890.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLLYHQ"
     gene            complement(398..1006)
                     /locus_tag="POH73_000920"
     CDS             complement(398..1006)
                     /locus_tag="POH73_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDI6886891.1"
                     /translation="MQLFETMKIDNGHIPRITYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHNGQYRLKIVLHAESEFETIVSPLPEKSSFTAKFQVLPKAVNPTFINNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRIFLINSLREVADVHLCL"
     gene            complement(1006..2157)
                     /locus_tag="POH73_000921"
     CDS             complement(1006..2157)
                     /locus_tag="POH73_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HDI6886892.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHVMCTHSLAKDDIYAVAYSFENAESINSTYEHQT
                     TYVSKHHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEVYKTVFQMTSMVS
                     GTLKTNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(2141..2734)
                     /locus_tag="POH73_000922"
     CDS             complement(2141..2734)
                     /locus_tag="POH73_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDI6886893.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDIINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIT
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            3085..3753
                     /gene="queC"
                     /locus_tag="POH73_000923"
     CDS             3085..3753
                     /gene="queC"
                     /locus_tag="POH73_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDI6886894.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            3755..4174
                     /gene="queD"
                     /locus_tag="POH73_000924"
     CDS             3755..4174
                     /gene="queD"
                     /locus_tag="POH73_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDI6886895.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            4178..4891
                     /gene="queE"
                     /locus_tag="POH73_000925"
     CDS             4178..4891
                     /gene="queE"
                     /locus_tag="POH73_000925"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDI6886896.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVIAQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            4990..5574
                     /locus_tag="POH73_000926"
     CDS             4990..5574
                     /locus_tag="POH73_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886897.1"
                     /translation="MIVYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDESAENNNKIKKLMIKGLIIINI
                     SFIVMMVLTPYWYLYLIVYLIFLLFLLWQKVYKF"
     gene            5854..6294
                     /locus_tag="POH73_000927"
     CDS             5854..6294
                     /locus_tag="POH73_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HDI6886898.1"
                     /translation="MNTKYFLAAGAIITTLALGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            6636..7109
                     /locus_tag="POH73_000928"
     CDS             6636..7109
                     /locus_tag="POH73_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDI6886899.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            7084..7770
                     /locus_tag="POH73_000929"
     CDS             7084..7770
                     /locus_tag="POH73_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6886900.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            7770..8825
                     /gene="saeS"
                     /locus_tag="POH73_000930"
     CDS             7770..8825
                     /gene="saeS"
                     /locus_tag="POH73_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HDI6886901.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCSEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENKDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITT"
     gene            complement(8897..9880)
                     /gene="csbB"
                     /locus_tag="POH73_000931"
     CDS             complement(8897..9880)
                     /gene="csbB"
                     /locus_tag="POH73_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HDI6886902.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVARQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSIENDEKDTHKVYSK"
     gene            complement(10012..10851)
                     /locus_tag="POH73_000932"
     CDS             complement(10012..10851)
                     /locus_tag="POH73_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6886903.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(11065..12414)
                     /locus_tag="POH73_000933"
     CDS             complement(11065..12414)
                     /locus_tag="POH73_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDI6886904.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLDTIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVMLNYEFNEPRPTIGQSDEDEKSE"
     gene            complement(12635..13816)
                     /gene="nagA"
                     /locus_tag="POH73_000934"
     CDS             complement(12635..13816)
                     /gene="nagA"
                     /locus_tag="POH73_000934"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDI6886905.1"
                     /translation="MSELIIYNGKVYTEDDKIDNGYIHVKDGQIVAIGEVDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDKALINIAKYEVEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAAE
                     RGAKHVTHLYNAATSFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLTNGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(14119..16077)
                     /locus_tag="POH73_000935"
     CDS             complement(14119..16077)
                     /locus_tag="POH73_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6886906.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDIASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKVGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDHADDEATKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIKNATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFDPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKVMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YVFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALVVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(16083..17003)
                     /gene="pfkB"
                     /locus_tag="POH73_000936"
     CDS             complement(16083..17003)
                     /gene="pfkB"
                     /locus_tag="POH73_000936"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDI6886907.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDADVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLSIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTICVLDGE"
     gene            complement(17000..17764)
                     /locus_tag="POH73_000937"
     CDS             complement(17000..17764)
                     /locus_tag="POH73_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6886908.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYKQKYHFIGGTL"
     gene            complement(18014..18496)
                     /gene="ybaK"
                     /locus_tag="POH73_000938"
     CDS             complement(18014..18496)
                     /gene="ybaK"
                     /locus_tag="POH73_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDI6886909.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENAKHEHFVFVIPVSETLDMKKAAAMVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(18670..19122)
                     /locus_tag="POH73_000939"
     CDS             complement(18670..19122)
                     /locus_tag="POH73_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886910.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            complement(19419..20585)
                     /gene="norA"
                     /locus_tag="POH73_000940"
     CDS             complement(19419..20585)
                     /gene="norA"
                     /locus_tag="POH73_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HDI6886911.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADVSPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSVVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPAILTLVLAFGL
                     SAFETLYSLYTSYKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     IYSVIVLVLLVIADGYWTIMVISFVVFIGFDMIRPAITNYFSNIAGDRQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKQQDL"
     gene            20795..21217
                     /locus_tag="POH73_000941"
     CDS             20795..21217
                     /locus_tag="POH73_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6886912.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITNFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDWDIQRSFNDAVGLNLLDLQ"
     gene            21404..>21691
                     /locus_tag="POH73_000942"
     CDS             21404..>21691
                     /locus_tag="POH73_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HDI6886913.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNL"
BASE COUNT         7202 a         3897 c         3050 g         7542 t
ORIGIN      
        1 actacaatgg cttggtcacc ttgactatat attttcattt gtacacctct tcaatatttg
       61 gttactttac tttgattttt aattatagaa tcgattgaat agttcatttt acctactcat
      121 taaatttact ggtgatacaa taaatccatt gctcgcacaa caaaaggcat gagttcgtga
      181 attaatgcgt atacaaggat aaagcttata acagtagtaa ttgttgctat caaacgaaca
      241 acatatattc tattttcaga tagcaatcga ttcactaatc gataatttac gtatacaaga
      301 attaacaaca gcacaatata aacaataatc atatttggcc ccatttaatt tttaaatttg
      361 ttttaacttc attttctaca ttattatcat actaatttta aaggcaaagg tggacatcgg
      421 caacctctcg taaactattt atcaaaaata tacgtatctc attgttatga tatttataaa
      481 tcaattcgtt tttattaaag tctttttcaa caagtttgtc actatctatt aaataatcac
      541 gcatgcatcc ttttaagaaa tcatctttat aacttggtgt gtaccatttt ccatcctctt
      601 caatgacaat gttgccaata tcaaattcaa ggaccttgcc gtcctctgaa gttagcaata
      661 ttaaatctgt ttcatgattg tgtgctaaat gctttcgttc tgtcgtttta ttatttataa
      721 aagttggatt aactgctttg ggcaacactt gaaattttgc tgtaaaacta cttttctcag
      781 gtaaaggtga cactatcgtt tcgaattcgc tttcagcatg taatacgatt ttaagtctat
      841 attggccatt gtgatacttt gttgttacat cgtttaattc atttcgccat gcatgttcat
      901 caaatttaaa gtttaatcgc tcagaagaac attttatgcg attagtatga taagtaattc
      961 tagggatatg tccattatca attttcattg tttcaaataa ttgcattata acatctccaa
     1021 aatcttggtt tttgcgtaaa attcatttac ttcatctttt ggcttagaat caattgtaat
     1081 acctgctccg actccataaa tcgcttgtcc atatttatac tcaatagtac gaattggaat
     1141 attaaaaatc attttatcat cttcagttgg aagtaatagt ccaattgctc cgcagtatat
     1201 accacgaggt gaactttcta attgtttaat atatttcatt gtattcagtt tcggtgcacc
     1261 tgtaatcgaa ccacaaggaa ataacgatgt taaaatttga gttaagtctg tattagtttt
     1321 taaagttcca cttaccatcg aagtcatttg aaatacagtt ttatatacct caatgaaaaa
     1381 tagtttatac accttaattg agccactctg tgatattctc cctatatcgt ttcttagtaa
     1441 atcaacaatc atgacatttt ctgcacgatc tttcgaagaa gtttgcaatg ttttataata
     1501 ctgttgatct tcagcttccg ttttacctct aggcattgtc cctttcatcg gtttgcttat
     1561 gataacgtta tcgacattgt taaactgtcc tttttgaaaa aataattctg gtgagataga
     1621 cgctacttgg atttcatctg tttgtaataa cgcagtataa ttaccattac taaattgagt
     1681 taatcgttca tataaagtac taataggata ataaatgttg tccgttaagc gcgccgtata
     1741 gttcacttga tacgtttcgc cttcaacaat tgcttgttgg acacgtttaa tattagtcat
     1801 cataacctca gaagattcaa caaatgaaaa atgatgtttt gatacataag tagtttgatg
     1861 ttcatatgtt gaatttatgc tttccgcatt ttcaaaacta taagctactg cataaatatc
     1921 atctttagct aatgaatgtg tacacataac atgattaaaa tactttgccg cttcgtaact
     1981 taaatataac gaaacatatc taccttgtcg ttgctgcgct tgtgcaaagt gtatcacttc
     2041 tcccacatca gccaacttag tggcaacata cttctttata aatcccttta attgaatatg
     2101 gtattgctta tattcatttt cagttaaata gtagcgataa ttatattcta ttctcacagt
     2161 aatcacctac cttcgataaa aataattcaa cttgtcgata accgtattca ctcaaaatag
     2221 attcaggatg atattgcaca ccaaaaaccg gaaatgtaat atgctcaaat gccataataa
     2281 ttgcttcatc gttttttgct gtaatcttta agcaatttgg aaaagtcgct ccgtcagcaa
     2341 ttaatgaatg ataacgcatt acattgaaat tttgaggcag tccttgaaaa ataccttcat
     2401 tggtatggcg taactgtgta gtatgtccgt gtacaggatg atagccgtga atgatatttc
     2461 caccaaaata agacacgata cattgaaatc ctaaacatac acctagtata ggtacacgct
     2521 gataaaattg ttctaacact tcattcaaga taggataatc atccggatta cccggcccag
     2581 gcgaaataac aattgctttt ggcttcatat taatgatgtc ttctatcagc agattatcaa
     2641 taccaacaac ttgaactgtt agtttcgttt gagtcttaat atagtctatt aaattatatg
     2701 taaatgaatc attattatct atgactagaa tcattgtata ctccgttcta aatgtgtttt
     2761 atttttataa tatgtattgg atgtagctaa aactttaaag gcattgtcat tatcctgacc
     2821 ttgattttaa ctactatatg gtatattcta ttcatcgtac ataaatgaat atcagaggtt
     2881 cctagctgaa accctctata aaaaactaga cattgaaatt tcaaacattc gtttgtaaac
     2941 ctttaatata tattatagga gcaacccatt tctttcgtct atcttttatg gagataggct
     3001 ttttttattt tataaattaa aaacgggctt atcaatgcca ttaacctctt tattaatatt
     3061 attaatgata ggagcaatta caaaatggaa agtgtattaa ataatgaaaa agccatcgtt
     3121 gtctttagtg gtggtcaaga cagtacaaca tgtctctttt atgcaaaaaa acatttcaaa
     3181 gaagttgaac tcgtaacgtt taattatggt caaagacatg atactgaaat tgaagtcgca
     3241 aaacaaattg cacaagatca aggaatgaaa catcacgttt tagatatgtc attattatca
     3301 caacttactc caaacgcatt aacacaacat gatatggaaa ttactaataa tgaagatggt
     3361 atacctaata catttgttcc agctagaaat ttacttttct tgtcgtttgc aggtgctcta
     3421 gcttatcaaa ttggggctaa gcatattatt acaggcgtat gtgaaacaga cttttcaggc
     3481 tacccagact gtcgcgatag ttttataaaa tcaatgaacg taacattaag cctagctatg
     3541 gacaaagatt ttgttattca tactccttta atgtggttaa acaaagcaga aacgtggaaa
     3601 ttaagtgatg aactcgaagt tttagattat attcgtacaa aaacattaac atgctataac
     3661 ggtatcattg gggatggctg tggtgaatgt ccagcttgtc atttacgtca acgtggacta
     3721 aatcaatatc ttgaaagtaa aggagcgctt taaaatgtta caacaaatct atcctagtac
     3781 aacgcatcca tatcaattcg aattaaataa agattttaat ttttcagctg cacatcacat
     3841 tccgtgtgaa gaagcaggta tttgtcaaaa tgtccatggt catacttact ttgttaattt
     3901 aacaattgtc ggtgataaac tagatgacac tggcttttta gtgaacttta gccatttgaa
     3961 aaagatgata cacggtaaat ttgaccatca actgttaaat aacttacctg cttttaaaaa
     4021 caaaatccct tcaactgaaa tcgtagcgga aacaatttat caaattgtta aagaaaattt
     4081 ggcatcgctc gaacaccaac caaaatgtat tcaagtattt gtaagagaaa caccaacaag
     4141 ttatgtcgta tttagaccaa aggaacaggt gtaactaatg gctaaaattc ctgtactcga
     4201 aatattcggt ccaaccattc agggtgaagg tcgagtaatc ggtagaaaaa cgatgtttgt
     4261 gagaacagct ggatgtgatt atcgctgtag ctggtgtgat tctgcattta cttgggatgg
     4321 tagtgctaaa ggcgatataa aactcatgac cgctgaagaa atttatgatg aattaaaacg
     4381 aattggtggc gatttattta accacgttac aatttctggt ggtaacccag cattaattaa
     4441 aggtatccaa gagttagttg acttatttca agataaaggt attttcagcg cactggagac
     4501 acaaggcagt aaattccaac cttggatgac acaaattgat gatttaacga ttagtccaaa
     4561 accaccaagt tcaactatga cgcctgattt aaaaaagtta gatgaagtga ttgcacaatg
     4621 cgtaccttcc tcattaaact taaaagtagt tgtattcgac gataaagatt atgattttgc
     4681 caaaatgata caccaccgtt atccagatat tcccttctat ttacaagttg gtaacccata
     4741 tttatcagac agcgtagata atcataccga aaagttgtta gaacgctacg agcagttggt
     4801 agatttagtt atgcaaagta atgatatgaa tcacgtttat gtgttaccac aacttcatac
     4861 attactttgg agtaataaaa aaggtgtata aaggataact tattttactt tttacaagtc
     4921 atatctaatg aataacaata tattaattga tataatagtt gattgaagta taacgaacag
     4981 gagtccatca tgattgtata tatattaatt aatattgcta tcgtcgtact tataactgga
     5041 ttcaatttat ataggcatca aatgcaacat ttatcattaa gtgcaatgtt gttatctatt
     5101 actataaacg cctttataaa tacattcata attgataaat ataactttat tactctgtgt
     5161 actataacga tgtttatcat ttggacgata ctacagtttt acattgataa aaaattaaaa
     5221 cctgtataca ttacagacca aaaatttatt gctatcatat taacgattgt tgtcagttta
     5281 acgcaacgtg ttaccgactt ttcaagtaca caatcaatat atatgtctat tccattttta
     5341 gcccctgcca ttttcatcat tggtggtatt atgttattta tttcaacatt taatagctta
     5401 gatgaatctg ctgaaaataa taataaaata aagaaactaa tgattaaagg acttatcatt
     5461 attaacattt catttatcgt tatgatggtt ttaacaccat attggtactt gtatttaatc
     5521 gtctatctta ttttcttgtt gtttttattg tggcaaaagg tttataaatt ttaataccaa
     5581 aactaaaaaa cacttctgat attcttagtt cgctatatca gaagtgtttt atagtgataa
     5641 ctagtttaga taaatatttt ctcacttaaa aaaccgccct cctcttattt tgactcctat
     5701 ttatttaaat cagacaatta ttttcatttt caaattattc tttcttcaat attagttaag
     5761 cgatatttaa acgaagttaa gaattagtta atggcatatt atttgccttc attttaaact
     5821 taacttatca aattgaagaa atgaggagtt agcatgaata caaaatattt tttagcagct
     5881 ggtgctatta tcacaacatt agctttaggc gcttgtggta attctaattc acaagatcaa
     5941 ggtaacaaaa ctgaacaaaa aacaaagtca gaagatagca atgttaaaac tgataaaaca
     6001 aaacacctaa caggtacatt cagttctaaa aacggtgaaa ctgttgaagg taaagctgag
     6061 attaaaaatg gtaaattaat gcttactaac tacaaatcat caaaaggtcc agatttatac
     6121 gtctacctaa caaaaaatgg cgacattaaa aacggtaaag aaatcgcaat ggttgactac
     6181 gataaagaaa aacaaacatt tgatcttaaa aatgtagatt taagcaaata tgatgaagta
     6241 acaatctatt gtaaaaaagc tcacgtcatc ttcggcggcg ctaaattaaa ataatcaatt
     6301 tctgagttaa acttttattt acaacatact attactatac attacaaatt ttaaaaatat
     6361 acattacact cattactcaa tggaaagcgt atgatttcac agccccccta gcttgtagaa
     6421 atcatacttt ccttttttca atatatatac aactattaaa tcccataaga ttgtagagca
     6481 cataagtaaa tttttttaga gcttgaggtt tgtttagctt aagcaaccca tgagctcaaa
     6541 cacttcctgt tcacataaca ctacaaatca cattatgttg ctaaatctta tgtttatata
     6601 aattacacac aataaataga aagaatgtga acatcatgaa taaattattg ctactcgtta
     6661 catttatcat tcgtgtgggt tcaggtattg ttatgttaat gcaaggttac gaaaaattaa
     6721 cgggcggatt tacgctgaaa ggtttagtac cagtcatcgc taacaatact gattcaccag
     6781 agtggtataa gtggtttttc gcaaatatag ttgcacatac gacgtcatta tttgatattg
     6841 ttgtcccact cggagagatt gcaattggat taggtttaat ttttggagtt tttgcatatg
     6901 ctgctagttt ctttggagcc tttgttatga taaattatat cttagcagat atgatattta
     6961 cgtatcctct tcaattaact ttctttatcc ttttattaat gagtcactca ttgttaaaac
     7021 agatttcact taaagaaatc attaattact ttagaggtcg taagaacaga ggtgaaaaaa
     7081 tagatgaccc acttactgat cgtggatgat gaacaagaca ttgtagacat ttgtcaaacc
     7141 tattttgaat atgaaggtta caaagtaaca acgacaacta gcggtaaaga agcaatttct
     7201 ttactatcaa atgatattga tatcatggta cttgatatca tgatgccaga agttaatggt
     7261 tacgacattg tcaaagaaat gaaaaggcaa aaattagata tcccctttat ctatttaact
     7321 gccaaaacac aagaacatga taccatttac gccttaactt taggtgcaga tgactatgtc
     7381 aaaaaaccat ttagtccaag ggaactcgtt ttacgtatta ataatttact tacaagaatg
     7441 aagaaatacc atcatcaacc agttgaacaa ctgtcatttg atgaattaac acttattaac
     7501 ttaagtaaag ttgtgactgt aaatggtcat gaagtcccta tgcgtattaa ggaatttgag
     7561 ttactgtggt atttagcttc tagagaaaat gaagttattt ctaaatcaga attacttgaa
     7621 aaagtttggg gatatgacta ttacgaagat gctaataccg tgaatgtcca tatacaccgt
     7681 attagagaaa aattagaaaa agagagcttt acaacatata ccatcacaac tgtatgggga
     7741 ttaggatata aatttgaaag gagccgataa tggtgttatc aattagaagt caaatcatta
     7801 ttggcgtcgt atcgagtata ctattaactt caactatttt agcaattgca tatattttaa
     7861 tgtggtttaa cggccatatg acactaactt tgaccttaac gacaataatt acaagctgtt
     7921 taaccttatt aatatgtagt atttttatta atccacttat acaaaaaatt aagcagttta
     7981 atataaaaac taagcaattt gctaacggaa attacgcaag caatgataaa acgtttaatt
     8041 caccaaaaga aatttatgaa ttaaatcaat cttttaataa aatggcttct gaaattacgc
     8101 aacaaatgaa tcaaattaaa tccgaacaac aagaaaaaac agaactgatt caaaacttag
     8161 cccatgattt aaaaacacct ttagcaagca ttatttcata ttctgaagga ctacgtgatg
     8221 gtataatcac aaaggatcat gagattaaag agtcatacga catattaatt aaacaagcaa
     8281 acagattatc aacattattt gatgatatga ctcatattat cactttaaat acaggtaaaa
     8341 catatccccc agaattaata caactagacc aattacttgt atcaatattg caaccatatg
     8401 agcaacgcat caaacatgaa aaccgcacat tagaagtgaa tttctgtagc gaaattgatg
     8461 cattttatca atatcgaacg ccacttgagc gtattttaac aaacttactt gataatgcgc
     8521 taaaattttc aaatgttggt agtcgcattg atattaatat tagtgaaaac aaagatcaag
     8581 atactatcga cattgctatt agcgatgaag gtattggcat tataccagaa ctacaagaac
     8641 gtatattcga acgtacattc agagtagaaa actctcgtaa tacaaaaacg ggtggttctg
     8701 gattaggctt atatatagct aatgaactcg cgcaacaaaa taacgcaaaa atcagtgtaa
     8761 gcagtgatat agatgtagga actacgatga ctgtaacatt acacaaatta gacattacga
     8821 cataatccga tttatttata aaataaactg caaagactaa aaagaagctc ccattaatga
     8881 gggcttcttt ttttgtttat ttagaataaa ctttatgggt atccttctca tcattttcaa
     8941 tacttgaaag atttgtagct tgaataatgt atttaggacg tgccttaact tcataatata
     9001 tcctgccaat atattcacct acaacaccaa ttgaaattaa ctgtatgccg cctaataata
     9061 aaatagctgc aatcgttgaa aaatatcctg ggatattaac accagaaatc ataatattga
     9121 tgaataaata gataatatat aacacgctta ttgaaaaaat aaataagcca agatatatca
     9181 tcattctcaa aggtttacta ttaaaggaaa tcaatccatc aataccataa ttaaataact
     9241 ttttaaagga ccatttagat tctccttttt gtctcgcaac attttgatac gtaaacactt
     9301 tagtattata tcctatccat tcaaataacc cttttgaaaa tcgattatat tcttcaagtg
     9361 atgcaatgga tttaacagct ctttggctta aaagtctaaa atcaccaaca ccatcatcaa
     9421 attgtacttc ttctacaaag caattaacta acttatagta caaatggctt aatgttttgc
     9481 gactaaaatt ttcaccactt ctatcacgct ttgcaatcac ttgatcatag ccttccataa
     9541 aaccttcaac cattttcgga atatattcag gtggatgttg caaatcacaa tctatcatga
     9601 tgactgcatc aaattcagta ctatgctggt aacctgcaat catagctgct tcttttccaa
     9661 aatttctact aaaagaaata aagctgacat gtctatctat tgtggcaaga tgttgcattt
     9721 catctatagt ggtatccgta ctaccatcat ctatgaaaag catattataa tcatagcctt
     9781 tcacactact atcttgtgaa agtatttcag ttaattgttg atgtgtttgt gtaatgactt
     9841 ccccttcatt aaaacaagga atgacgactc taattttcat ctatgtccat cctttttatt
     9901 taaaagattt tgaatgtacc tgagtattgt tttatcaaat agtaaaaacc aactcattta
     9961 ttttaatgta cacattaaaa ggcattcata aacgtaaaat ttaacatcca gttattttaa
    10021 atccccaatt ttcacatcat ctggattttt accttgtctt gcatttctat ttaaagcatc
    10081 aatttcagct acttctgtta attctaaatt aaaatctaaa atatctatat tttcttgaat
    10141 gcgtttaggt gtctgagatt ttggaataat aattctattg tgtgctaaat gccaacgcaa
    10201 tacaacttgt gctggtgttt tatgatattt ttcagcaatt ttaacaatga cagggtcgtc
    10261 tagtagtcct ctatttctca tcaaaggcat ccatgcagtc actttaatat cgtgacgatc
    10321 acaaaattct tgtaattctt gttggttaaa atatggatgt acctcaattt gattcaccat
    10381 tggtttgata cttgattgag ccattaattt ttctagatga tgaacattaa aattacatac
    10441 acctattgct tttaccttac cttgctcgta aagttcttcc atagctttat atgtttctaa
    10501 aaatagacca tctgcttcac aaggccaatg tattagaaat aaatcaagat aatcagtttg
    10561 taaattttca atcgatttgt tgaaatattc gaatgttttc tcataacctt gatagtcatt
    10621 ccataacttc gttgttataa acaaatcttc tctatcgacg ccattatcct ttaatgctcg
    10681 tcctagtgaa gcctcattat cataaaagta tgctgtatca aacgctctat agcctgcgtc
    10741 aattgcagca tttacaactt tagtcatatc ttcgtcagaa attttataaa cacctaaccc
    10801 aactgaaggc atcgggtatc cattatttaa tatttgtatc tcattcaaca tctttatatc
    10861 tctccaatct atgtatcttt tatatcttta cattacccta aatttttcaa caaactcaat
    10921 taatacgaat tatcgctttc aataaaaaat attcattaaa gatattgagt tccaatacta
    10981 ttttcacttt tcattaattt tagtcaaaaa aataaccaac caaaaatgaa ttaaatcatt
    11041 cttagttggt ttatatatta ttatctattc tgatttttca tcttcatcag actgtccgat
    11101 agtaggtctc ggttcattaa attcatagtt taacattacc caaataattt ggtggttatc
    11161 gatttctgaa acaacccagc gatcatacgt tgtatccacg taatcatctt tttgtaagtt
    11221 tgtattgcga gattgtaacc atccacctat cgtatcaata tcctcagagt catcaaattc
    11281 tataccgaac tcttcagtta aatcatccaa tagtactcta ccatttactt ggaatgtctt
    11341 attatcaatt ttaacgatat cattcacttc atcatcatca aattcatcac gaatttcccc
    11401 aacgatttct tctaaaatat cttccatcgt taaaatacct gccgttccac catattcatc
    11461 tataataaga ctcatatgta catgctcacg ttgcattcta attaatgcat cactgatacg
    11521 tgttgtctct gaaatcattg gcaactcgtg tatatagttt gctattttaa tcgtttttcc
    11581 agaagcatat tcagttaaaa attctttaac gttgataaat cctttaatgt ggtctttatc
    11641 accatcatca gtaattggat aacgtgtaaa ttgatgttct tttattgtat caagtaattc
    11701 gtctacatta aaaggttcat ttagtgtaat catttgcgtt ctaggtacca ttatatcttt
    11761 tgcatgtctt tcatcgaatg aaaagatatt ttgcatatat gccaattcag tttggttgat
    11821 ttctccacca ttataactat tgttaataat aattttgatt tcttcttctg acattgcatc
    11881 agtttgggca tcaggattta caccaaacat tctaataata acacgtgcag aaccattcat
    11941 cagccaaatc aatggtttca taatgttacc gaaatagaac aatggtcttg catatactaa
    12001 agcaagcttt tcagtatgtt gaatcgctat agatttaggc gctaattcac caagtactac
    12061 atgcaaatac gtaacgatta taaatgatac tgcaaacgaa atcgtcgtcg ttaatgcagt
    12121 tggtaaattg attgcttcaa atatcgggtg taatagcttt tcaaacgttg gttcaccaag
    12181 ccaacctaac cctaaagatg ttacagttat acctaactga caagcagaaa gataataatc
    12241 tagattagca atcatctttt ttactatttt agcaggttta tttccttcat ctgctagctg
    12301 ttcaattctt gttgctctaa tttttactaa tgcaaattct gaaccaacaa atactgtggt
    12361 taatgcaatt aatagaataa atataatcaa actaattatg gtcgaagttt ccaattaatt
    12421 ccctatttct agggattcac ctccatgtta tatgtgtcac tcatgggtaa cacgcattca
    12481 aatttatcac tatgacttaa attacaacta catgttatcg ccttcccatt tagacacccc
    12541 cagaaaaaaa tgttattgcc tctattttat cataatataa agtgcttatg ttaacagatt
    12601 aaatctattg catacattta attatgatta tctattagct aaatgtgtga accttacctt
    12661 gttttattgt agattttaca ttcatgtcat catctagaat aacaagatct gcatccttat
    12721 ttactttaat actacctttt ctatcatcga tacctaatgc aatggcttga tttaaacttg
    12781 ttacacgcca taaatgatct aatgtatcac ctgtaaatga tattaagtta cgtaacccat
    12841 gattcatttt taaaatacta ccagcaagtg caccatttgt aagacgtgct tgttgcgatt
    12901 gaacagttac tttttgacca cccaaatcat attctccttc aggcatacct tttgcacgca
    12961 ttgcatcggt aattaaataa aaacgttcat tacctttcat acggtaagca attgcaaccg
    13021 atgccggatg agaatgtgtg ccatcaacaa tcatttcggt atgtaaagca tcattcaacc
    13081 atgctgctcc aaaaacacct ggttctctat gttggaatga cgtcgctgca ttatataaat
    13141 gcgtgacatg tttagctcct cgctcagcag cttcaactgc ttcttcgtat gttgccactg
    13201 tatgaccaat tgaaaaaata atgtcatctt tatacgtttc aagcgcttct tttgcacctt
    13261 caacttcagg tgcaaacgtc ataatcttta ttagtccgtt agcagtctct tgaaaatgtt
    13321 taattttatc gataaatggg cgtacaacgt attgcggatg ttgagcacca actttatttt
    13381 cagatataaa tggcccttcc aaatgtatac ctacaatttc cgctgcattg tgaacatctt
    13441 gctccacttc atatttagca atatttataa gtgctttatc tattttatca gtcgattgcg
    13501 tcattgtagt ggccaagtac gatgtcgtcc cttcagacaa caaattttcg gatagatatt
    13561 ttaagccatc gtatgaccca tccattgcat cctgtccata accaccatga atatgtatat
    13621 cgataaaacc tggtaataca tgatgacctt ttgcatcaat cacttgaatt ttatttgtcg
    13681 tatcattatc aatcgctgct ttatcatcca cttctccaat tgcaacaatc tgtccatctt
    13741 tcacatgaat gtaaccatta tcgattttgt catcttcagt ataaactttg ccgttatata
    13801 taattaattc tgacacgtat aaacccttct ttatttataa attttcactt taagacactc
    13861 atattttact atatctgctt cgtttggcat aactttttgc aaaaacttta aaaactatat
    13921 ttatttttgt aatgactaca ttcacatact aaaaattaaa aacaccttcg catccattca
    13981 taaaaactga attaatggaa ggtgttttaa tagttttata taaactaccg ttaacacgat
    14041 atattaaatt aacatacatt caatcgactg acaaattgat gtaacgtcat ttctaacaat
    14101 cattttaaat cataaaagtt attcgtccat tgattttgaa gcttcgattt ctgtttcagt
    14161 taactttggt ttaataagtc cataaattaa tgctgaaact aatgtaccta ctacaagtgc
    14221 aataagtgtt tgtagtaaat gtgcaccatc cgtaccaaca attacgataa taccaccgtg
    14281 tggcgcagta attcgagaac ctaaacctaa agcgattgcg ccacctatac ctgacccaat
    14341 catcattgaa gggataacac gtaatggatc ggcagccgcg aatgggatcg caccttctgt
    14401 aataaacgac atacccatca catagttagg gataattgaa ccacgttgtt cttttgtaaa
    14461 tttacgtctg aaaattaaca tcgctgtcgc aattgctaac ggtggaatca taccaccaat
    14521 cattgcagct gtaattggtg ctgcattacc ttcaatcaac gcacctgttg caaaaacata
    14581 tgccgctttg ttgaatggac cgcccatatc aatcgccatc atagcgccaa tgactaaacc
    14641 taataataca atattagaac ctgataaatt atttaatcca tctaataata aatgatttaa
    14701 ccaagatgct ggtggattaa agacataaat catcaataag cctgtagctg tcactgttaa
    14761 taatgggtaa attaatgttg gttttaatcc ttctaatgct tgtggtaact tacgtgtcat
    14821 cactttaaca ccttgtgtta agtaacctgc taagaaacct gcaataatac caccaataaa
    14881 tcctgaacca cctgaaattg ctaacatacc acctacaaga cctgaagcga atccaggttt
    14941 atccgcaata ctacgtgcaa tgaatccaga taaaattgga atgattaacg cgaatgcact
    15001 tttactacca atgttccaaa gctgctctgc aaacgcattg tactctgagc tttttggatc
    15061 aaatgagttt gcaccaaata aaaacacgat tgccattaaa ataccaccag atattacaag
    15121 tggcaacatg ttagaaacac cgttcattaa gtgtttatag aaagctttac ctgggcttaa
    15181 tttttcttga ctgtcatttg agttaccttt tctttgacca tcacgggcaa caaaaggttt
    15241 acgacttgta tctaatgctt tattaattaa ttcttctggg cgtttaatac catctgctac
    15301 tggtacttct acgacatttt taccatcgaa gcgatccgtc tcaacatgaa catcagcagc
    15361 aacaatgata cctgttgcat ttttaatatc ttgttccgtt aaatggtttt taataccact
    15421 tgaaccgttc gtttcaactt taattttaat gcccatttta tccgcttgct ttttcaatgc
    15481 atcacgtgcc atatatgtgt gtgcaatacc tgttggacaa gcagttacag ctaatacata
    15541 tggctcgtta gattgtttag atgattgttc tgcagttgca acttgttgtg cttcagcttc
    15601 tgcctcttct tcttttgtcg cttcatcatc agcatgatca ataattgcta acacttcttc
    15661 tggagacgat gcatgtaata atttttcacg tacattttca tccattaaaa taccagacaa
    15721 cttagctaaa gcatctagat gtgtttgtgc gccaccttct ggcgctgcaa tcataaagaa
    15781 taagtgtgct ggttgcatat ccaaactttg ataatctacg cctactttag atttaccaaa
    15841 tgcaatagct ggtgacttaa ctgcggccac tttggcatgt ggaatagcaa taccttcacc
    15901 gataccagtt gtactttgtg attctcgatt gtgaatcgct tccttaaatg acgcgatatc
    15961 acttaattta cctgctttgt ctaattgatt tactaactca tcaataacac catttttgtc
    16021 atttgccatt aaatccattg ctatcgtatc ttttgttaat aactctgtta ctctcattat
    16081 tttcactccc catcaagtac gcaaatcgta acttgtgatt ttattttttc tatagcatcc
    16141 cgtgttgcta agtcctcatc aaatgccgtg gcagtaccgc atgcgactgc ttgttgaaat
    16201 gctttttcaa tcgataaacc tgaagcaatt ccagccacca tgcctgcaac tgtactatca
    16261 ccagagccaa ctgtattaac cactttccct tgtggattaa ctgctttaat actgatttct
    16321 ttatcaatat aaatagcacc atcaccgcca agtgagacaa taacagattg cgcaccttta
    16381 tcaactaaca aacgaccata tttaataaca tctgcgtctg agttcactgt tgtattaaac
    16441 atcacttcta attcatcttt attaggttta ataaatagtg gatgatatgg taaaacgctt
    16501 tcagctaatt ctttttcagc gtcgactact aatttagcac ctgtctgtgc tgtaatttgt
    16561 gcaatttgtg catacgcatc gcttggaata ctacttggta cacttccagc aacaataact
    16621 atatcttcgc ttgttgtatt tttaatttgt tgcaacagtt gttcaaattg tgttgacgtt
    16681 atatgaggac ccggtgcatt gatttctgtt tcttgtcctg tttttaattt cacattaata
    16741 cgtgtatctt catcaacttc aataaaattc gattgaattg cactgttatt taatgtatct
    16801 ataatgaatt tcccaggaaa tccacctgca aatcccaaag cagttgactc aacatccaat
    16861 gtctttaaaa cacgtgagac attaatacct tttcccccag cgaatttata tgttgctgtt
    16921 gctctgttca aaccatcaat tttaaaatca ttcgtaaaaa tgacatagtc aattgaaggg
    16981 ttgaaagtca ctgtataaat cataaagtcc ctcctataaa gtgatacttt tgtttgtatt
    17041 cttttaacga ttcttgattt aatgcttttt cagatgtgat gattgtcgta ctttctagca
    17101 aaggtacacg agcaaaatat actttattaa acttagaatg atctataagt acaaatgatt
    17161 gattggctaa tgacattgct gtttgtttaa ctaatgcctc ttgctcatcg ggagtagtta
    17221 atccaagttc aatatctaat ccattcatcc cgataaaagc tttatcgaaa caatatcgtc
    17281 ttaatatctc catggcactt gaaccaatcg tagcaagtgt attttcttta acttgaccac
    17341 ctagcataat tgttttaata ccttttttaa gtaaagcttc tacatgtgtt aaaccattgg
    17401 ttaccacaat gatatctttc gcttgaatat atttaattag ctccaatgta gatgaaccag
    17461 catcgataaa taagcattca ttatcgttga tttgattagc tgctatttta gcaatcattt
    17521 tcttttcatc gagattagtt gctaattttt cagttaaatt cgcctcaacc atacgatttt
    17581 cttttaacat tgcaccacca tgcacacgtt gtaatttccc taattgttgt agtttagata
    17641 aatctcttcg tattgttgaa gcactgcagc cagttcgatc tattaattct tgtaaagtca
    17701 aaaaatcttt gtgcgaaagt tcttctaata ttaactcgtg tcttttttct gtaattatca
    17761 tcatcacccc atctcactct gtattataat gagtacgctt tcacttttca atcataaatt
    17821 accaaaaaca atcacaaaca atcaaattgt gacgataaaa ttacattttt atgttcaaat
    17881 actcaattat catgactgtt tatgatcgta aaatgctatt tcaatcaaaa aggagtgtag
    17941 cagaaatgaa ttttataaaa atttcatctc tgtcgcactc ctaatcttgc cccatacttt
    18001 ttgttgttat taatcattca tggataactg ctccaatttt ccctttagtt attgtaatca
    18061 aatcctcaac agctatcgtg atttgcattg ttcgaagccc accactcaca ctgatatgac
    18121 tataattttc acaggatttg tcaacgactg ttggaaacaa tgttttcata ccaacaggtg
    18181 aacacccacc acgaatgtat cccgttacat tttttaaatt atctaaaggc ataagttgca
    18241 atttcttctc tccaaccata gcagctgcct ttttcatatc taaagtttca cttactggga
    18301 taacaaatac aaaatgttca tgttttgcat tttctagaac aagtgtttta aatactgttt
    18361 taacatttgc ttgtatgagt tctgcgactt cttggccaga taaatgttct tctggtacct
    18421 caaagctatg aacttcgtat ttaatttttg cacgatcaag catacgcatc gcgttagttt
    18481 tcttattttt agccatttca acacctcatt tatacttctt cagaactata ttaacatttt
    18541 aaaatacatt acggattggt tgattttgac ataacattta catcatttta taagttgatt
    18601 tcaccttttt aaattaaaat attgcaaaat aaaaacagta tatccaatta gaatacactg
    18661 ttccatctat tatttcgtta ttatgccttg atttgaaata cttacatgtt gcttactcgt
    18721 tgaattatca ttggaataaa cataattata tccatgattt gtcttagaaa ttctttctaa
    18781 accataatta tcattgatat gcttatctgc ataacgatca gcaataactt ggcactcttg
    18841 ttcgttaaaa gaatttgatt tatttgcatt cacacgatgc ttaaaatgat tttgcttttt
    18901 cacattttca ttcacttcag atgctttagc agtatgaata ttgagaatat ccattttctt
    18961 tttataagta tcttcagttg atacatttat cgcgttaagc gtattaatcg ttttatgtac
    19021 cttagcaagc gcatcttcat ttgttgctaa tattaataaa ccaattaaac aaacggttaa
    19081 tattacagca acaaagccta aaaataaatt ttttactgtc atccccttac ccacatttcc
    19141 ttactcattt tttatcatct taacacatta acgttaatga attaggtatg tggattgcaa
    19201 tttaaaaaga atgtaatatt tgtaacatgt ttattactca aaatcatttt ttgttacata
    19261 taacatttaa atataaagac taaaacattt aaaagttttt aatctttata aatttaaagc
    19321 atcgactttt cttcactata attgtaagaa aatcgatgct tattttgaaa tgctttagtt
    19381 gtcgaaatac attaatcgaa tgacaaccat gtgcgatatt acaaatcttg ttgttttaac
    19441 ttagctctat gttgcttttc aattaaaaca ataacaacac ctgctaatga cacacctata
    19501 gccatataaa ttggtgcttc aatgtgtaca tcaaataacg cacctgcgat taaaggacct
    19561 atgaaattac ccatactagt gaatgtcgag tttaacccac ctgcaaatcc ctgtctatca
    19621 ccagcaatat ttgagaaata atttgtaata gcaggtctta tcatatcgaa cccgataaag
    19681 acaacaaagc taattaccat aattgtccag taaccatcgg caataactaa tagcactaat
    19741 acaataactg aatatattaa tgaccatgca ataaatgtta actcagagaa gtatttcata
    19801 aatttatcaa agaaataaat ttggaaaagt gcaccaaaga taccgccacc cgtaatcgca
    19861 atcgaaatat ctttaggtga ataatttact ttatacgatg tatataaaga atacagtgtt
    19921 tcaaatgccg ataaaccgaa cgctaagacg agcgttaaaa ttgctggcgt aataaagact
    19981 ttccaattaa tttttgttaa taattggggc tcaagttttt ggaagccgct tgtcgtagac
    20041 tttttcggat catgaatcaa tacaactgac attatgaaag ctaaaatgcc taatgcacct
    20101 gcaaaataaa atggcatacg atgtgaaact tctgccataa atccaccaat ccctggtcct
    20161 aaaatgaatc cagaattgat aatcgctgac atgtagccaa agttttttgc tttttgatgg
    20221 cttggtgaaa catctgcaat caaacctgtc acacccggca ttaccatacc agcactcata
    20281 ccaccaatca ctctcgataa catcaatacc gaaaaattgt ggccaactgc aaacataaat
    20341 tctgacactg agaataaaat taaccctata catataatta atttcttccc taatttgtca
    20401 gctagcgtac caccaaacgg cgatataatc atttgagata acgcaaaagc agcaacaagt
    20461 aaccctaaat cactaccagt taatcccaaa tcttttaaat aaacaggcaa gactggtatt
    20521 actaaaccga tacctaaaaa aatcaagaaa atattaaaat ataagacaaa aatctgttta
    20581 ttcatatgct cacctctttt ttctccatgt cattcttaaa tctgttattc ttcttttcca
    20641 cgacaaattg caattcatgc taaatattcg taaaaagttt ctatattgta ttacaacatt
    20701 gctacatttg acaatatttt tttctttgta attatcacta ttttccattt aattgtataa
    20761 ttaatgacat attaaaataa aggggattga tcatatgaaa ttgcaaaaaa gtaatcatac
    20821 tatactacta gtgttagaaa aaggagaaga tatcgttgaa tgtataacaa actttgcaga
    20881 tgaccaagac ttaacattta catctgtcag cggcattggt gcatgtgatg acgtcgtttt
    20941 aaaatttttc aatttaacaa cgaaacaata tgaagaaaaa catattactg agccactaga
    21001 attgacgagc ttattaggta atatttctcg tctagataat ggacattttg cacatttgca
    21061 tgctactttc ggtactcaat catatgaaac ttttagcgga catctcgcta aagcaattgt
    21121 ttctgcaact gctgaaatca ttcttactgt aactgattgg gacattcaac gttcattcaa
    21181 cgatgcagtc ggtttaaatt tacttgatct tcaatagcac tttacttttt agttgaatca
    21241 aattttatta caagttatca taagtgctta atcataaatg cattctcgtt tattaatata
    21301 cttcaacgag ttaaattaaa tttttaatta ggacttgaat gcgtttataa ctatcacatt
    21361 tgaatgaata acttttttca ttagaaagta gtgtttatta ttgatgcaat ctcgatacat
    21421 cgctagaatt tactttttaa tattatttat cgtctccctt tttgagacta aaatttttca
    21481 atttatgact ttaaatttgt ttttagcata tatacctttt gagcttagtc tattattacg
    21541 attattcaaa ccgaataaaa agtatgaatg gccactcttt atcatatata gttttatatt
    21601 tgtgttactt ttaccaaata cattttatat ggtaactgat ttaatccatt tgaaccaatt
    21661 tcaattcaac ttttacgcag gactaaattt a
//
LOCUS       DAMZPP010000033        21548 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMZPP010000033 DAMZPP010000000
VERSION     DAMZPP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21548
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.033"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..138
                     /locus_tag="POH73_000943"
     CDS             <1..138
                     /locus_tag="POH73_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6886914.1"
                     /translation="SWISLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNA
                     Q"
     gene            135..896
                     /locus_tag="POH73_000944"
     CDS             135..896
                     /locus_tag="POH73_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6886915.1"
                     /translation="MIQVGNLTKTINNQMILDDISIEIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFDEGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHEIVNDALDLLQLQDIRKRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVAMKNGQLVKSD
                     LKDNVIQSSVLSDLYDMDIQIEHIRNQRICLYFKD"
     gene            1011..2039
                     /locus_tag="POH73_000945"
     CDS             1011..2039
                     /locus_tag="POH73_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6886916.1"
                     /translation="MKKTVLYLVVAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLISSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKKFNKS
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKNVSNSNHGQNVSNEYINKENPDV
                     ILAMDRGQAVSGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     DELEKVVK"
     gene            complement(2356..2670)
                     /locus_tag="POH73_000946"
     CDS             complement(2356..2670)
                     /locus_tag="POH73_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886917.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(2688..3611)
                     /locus_tag="POH73_000947"
     CDS             complement(2688..3611)
                     /locus_tag="POH73_000947"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDI6886918.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNVEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLVKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            complement(3738..4256)
                     /locus_tag="POH73_000948"
     CDS             complement(3738..4256)
                     /locus_tag="POH73_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDI6886919.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     TNHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYNKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDRLLSTNDEIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            4376..5254
                     /locus_tag="POH73_000949"
     CDS             4376..5254
                     /locus_tag="POH73_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HDI6886920.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            5408..5728
                     /gene="ytxJ"
                     /locus_tag="POH73_000950"
     CDS             5408..5728
                     /gene="ytxJ"
                     /locus_tag="POH73_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HDI6886921.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            6153..7277
                     /locus_tag="POH73_000951"
     CDS             6153..7277
                     /locus_tag="POH73_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDI6886922.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAHQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPESYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(7465..8691)
                     /gene="pepT"
                     /locus_tag="POH73_000952"
     CDS             complement(7465..8691)
                     /gene="pepT"
                     /locus_tag="POH73_000952"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDI6886923.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDADVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDTAENN"
     gene            complement(8705..9199)
                     /locus_tag="POH73_000953"
     CDS             complement(8705..9199)
                     /locus_tag="POH73_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDI6886924.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRSYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            complement(9217..9978)
                     /locus_tag="POH73_000954"
     CDS             complement(9217..9978)
                     /locus_tag="POH73_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDI6886925.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            complement(10174..11244)
                     /locus_tag="POH73_000955"
     CDS             complement(10174..11244)
                     /locus_tag="POH73_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDI6886926.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            11562..12617
                     /locus_tag="POH73_000956"
     CDS             11562..12617
                     /locus_tag="POH73_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDI6886927.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
     gene            complement(12781..13422)
                     /locus_tag="POH73_000957"
     CDS             complement(12781..13422)
                     /locus_tag="POH73_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDI6886928.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            13566..14432
                     /gene="fakB1"
                     /locus_tag="POH73_000958"
     CDS             13566..14432
                     /gene="fakB1"
                     /locus_tag="POH73_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HDI6886929.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            14796..15877
                     /locus_tag="POH73_000959"
                     /pseudo
     CDS             14796..15877
                     /locus_tag="POH73_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            15870..16544
                     /locus_tag="POH73_000960"
     CDS             15870..16544
                     /locus_tag="POH73_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDI6886930.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKYLDQNEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFSKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            16605..17177
                     /gene="raiA"
                     /locus_tag="POH73_000961"
     CDS             16605..17177
                     /gene="raiA"
                     /locus_tag="POH73_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDI6886931.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            17591..20122
                     /gene="secA"
                     /locus_tag="POH73_000962"
     CDS             17591..20122
                     /gene="secA"
                     /locus_tag="POH73_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDI6886932.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKSVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVADAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            20164..20262
                     /locus_tag="POH73_000963"
     CDS             20164..20262
                     /locus_tag="POH73_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886933.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSLKK"
     gene            20437..21547
                     /gene="prfB"
                     /locus_tag="POH73_000964"
     CDS             join(20437..20508,20510..21547)
                     /gene="prfB"
                     /locus_tag="POH73_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDI6886934.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDGEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
BASE COUNT         7784 a         3068 c         3828 g         6868 t
ORIGIN      
        1 agttggatta gtttatttag tgcgcaatgg gtagttgaaa atgtgtttga ggctacgaca
       61 gaaatgagta tacttattga tttaattggt ggaagttatt tcatttatct attagttaga
      121 aggagaaatg cgcaatgatt caagttggga atttaactaa aactataaat aatcaaatga
      181 tattggacga tattagcata gaaattgaaa aaggtaagtt gacttcttta attggaccta
      241 atggagcagg gaagagtacg ttactttcag cgatatgtcg cttaattcgg tttgatgaag
      301 gggaagtaaa aatagatgga cagcttatgt ctgattataa aaacaatgat ttgtcgaaaa
      361 agatatcgat tttaaagcaa acgaaccata cagaaatgaa cattactgta gaacaattag
      421 ttaatttcgg acgatttcca tattctaaag gacgtttaac gaaagaagac catgaaattg
      481 ttaatgatgc tttagatttg ttgcaattac aagatattag aaagcgtaat ataaaatcat
      541 tgtctggtgg acaacgtcaa cgtgcctata tagcgatgac cattgcacaa gatactgaat
      601 atattctttt agatgaaccg ttaaataatt tagatatgaa gcatgctgtt caaattatgc
      661 aaacgttaaa aatgttagcg cataaaatga ataaagcgat tgtcattgtg ttacatgata
      721 ttaactttgc gtcctgttat tcagatcaga ttgtagcaat gaaaaacgga caactagtta
      781 aatcagattt gaaagataat gtcattcaaa gtagtgtttt aagtgattta tatgacatgg
      841 atattcaaat tgaacatata agaaatcaaa ggatttgttt atattttaag gattgataat
      901 ttggagacac tttaaagggg tgatgcgcca attaaagaag ggttaacaag tatttattcg
      961 tatttcacat caagcacaca gattaagcca aaagaggaga atattatatt atgaagaaaa
     1021 cagtcttata tttagtagta gcagtaatgt ttttattagc ggcatgcggt aacaattctg
     1081 ataaagaaca atcaaaatca gaaactaaag gttctaaaga tacagtaaaa attgaaaaca
     1141 actataaaat gcgtggcgag aaaaaagatg gtagcgatgc taaaaaagtt aaagaaactg
     1201 ttgaagtacc aaagaaccct aaaaatgcag ttgtattaga ttatggcgca ttagatgtaa
     1261 tgaaagaaat gggcttatca gacaaagtaa aagcattacc taaaggtgaa ggcggcaagt
     1321 cattaccgaa tttcttagag tcatttaaag atgacaagta tacaaatgtt ggtaacttga
     1381 aagaagttaa tttcgataaa attgcagcaa caaaaccgga agtaatcttt atctctggcc
     1441 gtacagcaaa tcaaaagaat ttagatgaat ttaaaaaagc tgcaccaaaa gcaaaaattg
     1501 tttacgttgg tgcagatgaa aagaatttaa ttagttcaat gaaacaaaac actgaaaata
     1561 ttggtaaaat ttacgataaa gaagataaag ctaaagaatt aaataaagat ttagataaca
     1621 aaattgcttc aatgaaagat aaaactaaga agttcaacaa atcagttatg tatttattag
     1681 ttaatgaagg tgaattatca acatttggac ctaaaggtcg tttcggtgga ttagtttacg
     1741 atacattagg tttcaacgcg gttgataaaa acgtaagcaa tagtaaccac ggacaaaatg
     1801 tctctaacga atacattaat aaagaaaatc cagatgttat tttagcaatg gatagaggac
     1861 aggctgtaag tggtaaatca actgcgaaac aagcattaaa taatcctgta ttaaaaaatg
     1921 ttaaagcaat taaagaagac aaagtttata atttagaccc taaattatgg tactttgcag
     1981 ctggatcaac tacaactaca attaaacaaa ttgatgaact tgaaaaagtt gtaaaataat
     2041 tttaaaagag gggaacaatg gttaaaggtc ttaatcattg ttcccctctt tgctttaaaa
     2101 aagggaatct gggacgtcaa tcaatgtcct agactctaaa atgttctgtt gtcagtcgtt
     2161 ggttgaatga acatatactt gtaacaagct catttcaata cctgtgggct ccaaacatag
     2221 agaaattgga tttccaattt ctacagacaa tgtaagttgc cgggctccaa acatagagaa
     2281 tatcaaaaag aaattctaca gaagtggtgc tttatcatgt ctatcccact ccctataatt
     2341 ttttggctat gttgtttaaa tttcgaaata aatatgatag tgatatttgc agcgattgtt
     2401 aaatcgagat tggcaattag gacaacgctc aaccatcata tattcattga ttgttaattc
     2461 atgtttgcat acaccgcata agattgcttt ttcgttaaat gaaggctcag accaacgctt
     2521 aatggcgtgc ttttcaaact cattatggca cttatagcat ggatagtatt tattacaaca
     2581 tttaaattta attgcaataa tatcttcttc ggtaaaataa tggcgacagc gtgtttcagt
     2641 atcgattaat gaaccataaa ctttaggcat agacaaagct ccttaactta cgattccttt
     2701 ggatgttcac caataatgcg aacttcacga tttaattcaa tgccaaattt ttctttgacg
     2761 gtcttttgta cataatgaat aaggttttca taatctgtag cagttccatt gtctacattt
     2821 accataaaac cagcgtgttt agttgagact tcaacgccgc caatacggtg accttgcaaa
     2881 ttagaatctt gtatcaattt acctgcaaaa tgaccaggcg gtctctggaa tacactacca
     2941 catgaagggt actctaaagg ttgtttagat tctctacgtt ctgttaaatc atccatttta
     3001 gcttgtattt cagtcatttt accaggagct aaagtaaatg cagcttctaa tacaactaag
     3061 tgttcttttt gaataatgct attacgataa tctaattcta attcttttgt tgtaagttta
     3121 actaacgagc cttgttcgtt tacgcaaagc gcatagtcta tacaatcttt aacttcgcca
     3181 ccataagcgc cagcattcat atacacagca ccaccaattg aacctggaat accacatgca
     3241 aattcaaggc cagtaagtgc gtaatcacga gcaacacgtg agacatcaat aattgcagcg
     3301 ccgctaccgg ctataattgc atcatcagat acttcgatat gatctagtga taataaacta
     3361 attacaatgc cacgaatacc accttcacgg ataataatat ttgagccatt gcctaaatat
     3421 gtaacaggaa tctcattttg ataggcatat ttaacaactg cttgtacttc tacattttta
     3481 gtaggggtaa tataaaagtc ggcattacca cctgtcttag tataagtgta tcgttttaaa
     3541 ggttcatcaa ctttaatttt ttcatttggg ataagttgtt gtaaagcttg atagatgtct
     3601 ttatttatca cttctcagta catcctttct catgtcttta atatcatata gtattatacc
     3661 aattttaaaa ttcatttgcg aaaattgaaa agaaagtatt agaattagta taaatataaa
     3721 atacggcatt attgtcgtta taagtatttt ttacatagtt tttcaaagta ttgttgcttt
     3781 tgcatctcat attgtctaat tgttaagcta tgttgcaata tttgatgctt tttagtattg
     3841 aattgtaaag cgatctcatc attagtcgat aagagacgat caagtgcaag ataagattca
     3901 aatgtttggg tattcatttg aatgatatgt agacgcacct gttgttttag ttcatgaaaa
     3961 ttgttaaact tcgccatcat aaccttttta ttatatttat gatgcaaacg ataaaaccct
     4021 acataattta agcgtttttc atctaaggat gtaatatcat gcaaattttc tacacctact
     4081 aaaatatcta aaattggctc tgttgaatat ttaaaatgat tcgtaccgcc aatatgtttt
     4141 gtatatttta ctgggctgtc taagaggttg aataataatg attcaatttc agtgtattgt
     4201 gattgaaaac aattagttaa atcactatta atgaatggtt gaacatttga atacatgata
     4261 aactcctttg atattgaaaa ttaatttaat cacgataaag tctggaatac tataacataa
     4321 ttcattttca taataaacat gtttttgtat aatgaatctg ttaaggagtg caatcatgaa
     4381 aaaaattgtt attatcgctg ttttagcgat tttatttgta gtaataagtg cttgtggtaa
     4441 taaagaaaaa gaggcacaac atcaatttac taagcaattt aaagatgttg agcaaaaaca
     4501 aaaagaatta caacatgtca tggataatat acatttgaaa gaaattgatc atctaagtaa
     4561 aactgataca actgataaaa atagtaaaga atttaaggca ctacaagaag atgttaaaaa
     4621 ccatctcata cctaaatttg aagcatatta taagtcagca aaaaatttgc ctgatgatac
     4681 aatgaaagtt aagaaattaa aaaaagaata tatgacgctt gcaaatgaga agaaggatgc
     4741 gatatatcaa ttaaaaaaat tcataggttt atgtaatcaa tctatcaagt ataacgaaga
     4801 cattttagat tatacgaaac aatttgaaaa aaatagatac aaagttgaat cagaaattaa
     4861 attagctgat aataaaagtg aagcaactaa tcttacgaca aaattagaac ataataataa
     4921 agcgttaaga gatactgcga aaaagaacct agatgatagt aaagaaaatg aagtaaaagg
     4981 cgcgattaaa aatcacatta tgccaatgat tgaaaaacaa attaccgata ttaaccaaac
     5041 taatattagt gataagcatg ttaataatgc aaggaaaaac gcaatagaaa tgtattacag
     5101 tctgcagaac tattataata cacgtattga aacaataaag gttagtgaga agttatcaaa
     5161 agtcgatgta gataagttgc cgaaaaaggg tatagatata actcacggcg ataaagcctt
     5221 tgaaaaaaag cttgaaaaat tagaagaaaa ataactataa tcatttttca aagttaaaaa
     5281 ttttgaattt atggttaaca tgtcaactta ctatgtgtat aatggtaaac attgatatta
     5341 actatatgta taaaaatgtc acgcagatgc tatttaaatg tgataaatat ttttagaggt
     5401 gaatagagtg gctataaagc taagttcaat tgaccaattt gaacaggtta ttgaggaaaa
     5461 taaatatgtt tttgtattaa aacatagtga aacttgtcca atatcggcaa atgcgtacga
     5521 tcaatttaat aaatttttat atgaacgcga tatggacggt tattatttga ttgtccaaca
     5581 agaacgcgat ttgtcagatt atattgctaa aaaaacgaac gttaaacatg aatcacctca
     5641 agcattttat tttgtaaatg gtgaaatggt ttggaatcga gaccacggtg atattaatgt
     5701 gtcgtcatta gcacaagcag aagaataatg aaactatagg gttggaacat tttgccttac
     5761 actactagac gtgaatagca caacttaaat tcgtgtgaat cagagtagtt tggctataat
     5821 gatgttctga ccttttattt tatgtaacct ttagaagcag ttaagttagt acttttttac
     5881 aaacatatgt ataatgtatt cgagtatttt tattgaaaat attttgggaa acgacgaatc
     5941 caataagaaa attttaacat gatttgtaag ttagtttaat aggaaataca tgctaaacca
     6001 taagaagtat actgttattt gctggaataa ttaataatca tgtcatgtta aatgttcgca
     6061 tataatcacg agataaaatc taaaatttaa gattaatctt ttatgaataa aaaacgtatc
     6121 acaacaaata ataaagtaag gtggtcaagg ttatgaaagt attagtagcc atggatgagt
     6181 ttcatggaat tatttcgagt tatcaagcta atagatatgt tgaagaggca gttgcaagcc
     6241 aaattgaaac tgcagatgta gttcaagtac cattgtttaa tggaagacat gaattattag
     6301 attctgtatt tttatggcaa tctgggcaaa agtatcgtat accagtacat gatgcagata
     6361 tgaatgaagt tgaaggtgtt tacggacaaa ctgatacagg gatgaccgtt atcgagggga
     6421 atttattttt aaaaggtaaa aaaccaattg ttgaacgaac aagttatggt ttaggagaaa
     6481 tgattaaaca tgcattagat aacgacgcaa aacatgttgt aatttcatta ggtgggattg
     6541 atagttttga tgctggtgca ggtatgttac aagcattagg tgctcaattc tatgatgacg
     6601 aagggcgtgt cgtagatatg agacaaggtg ctggtgtaat taaatatatt cgtcgtatgg
     6661 atatgtcgaa cttacaccct aaaatggaaa cagcaagaat tcaagtaatg tcggattttt
     6721 caagtcgatt atatggtaag caaagtgaaa tcatgcaaac ttatgatgcg catcagttga
     6781 accataatca agcagcagaa atcgataatt taatttggta ttttagtgag ttatttaaaa
     6841 gtgaattgaa aattgcaatt ggtccagttg aacgtggtgg tgctggtggt ggaattgcag
     6901 cagtcttgaa tggactgtat caagctgaaa tattaaccag tcatgcatta gtagaccaac
     6961 taacacattt agaaaattta gttgaacaag cggatttaat tatttttgga gaaggattaa
     7021 atgaaaatga tcagttgcta gaaacgacaa cattgcgtat tgcagaactt tgtcataaac
     7081 atcaaaaggt tgccattgca atttgtgcaa ctgctgaaaa gtttgattta tttgaatcac
     7141 aaggggttac agcaatgttt aatacattta tcgatatgcc ggaaagttat accgacttta
     7201 aaatgggatt acaaattagg cattatactg ttcagtcttt aaaactgttg aaaacacatt
     7261 ttaatgttga ggtttagtaa agaaggacta aattggtgat gctgtcatga tggttaataa
     7321 catttatgat ggttagcaaa acgaattaga agatcgaaag tatacgtaaa aaatatgaaa
     7381 aatcacgcta tcattgcact gaatgttagc gtgatttttt atattaatta agcctgagtt
     7441 gaactagtat ataatcgttt gtttttagtt attttcagcg gtatcttcta caattccaat
     7501 gattacttgt actgcttttt ccatgacatc aatggatgca tattcatatg ggccgtggaa
     7561 gttaccgcaa cctgtaaaga tgtttggagt tggtaatccc ataaatgaca attgtgaacc
     7621 atctgtacca ccacgaatag gttcagtgtt tgctggaata tctaatttag caaagacacg
     7681 tttaggtata tcaataatat gaggcaatgg taatattttt tctgccatat tgaaatattg
     7741 atccgatata tcaactttaa ctggataatt ttcaaaatgg gcattgatat cgtcacgtat
     7801 ttctaaaata cgtttcttac gcaattcgaa ttgtttttta tcatgatcac gaataatgta
     7861 ttgcaaagtt gctttttcaa cagttccttc aaagttcatt aagtggtaaa agccttcata
     7921 tccttctgtt cgctccggaa cttcactatc aggtagcaaa ctatcgaatt gttcacctaa
     7981 acgtattgcg tttaccattg catttttagc tgaaccagga tgaacattca caccgtggca
     8041 tgtaataacc gcttcagcag cgttaaagct ttcatattgt aattctccat attgactacc
     8101 atccatagta taagcaaaat cagcattgaa gcggtcaaca tcaaatttat gtggaccacg
     8161 accgatttct tcgtctggtg taaatccaat gcgaatggta ccatgtttaa tttctggatg
     8221 ttcttgtaaa taacaaatag cttccataat ttccacaata cccgctttat cgtctgcacc
     8281 tagtaacgat gtaccatcag ttaccattaa tgtatgacca actaaactgt taagttctgg
     8341 aaatacttta ggatctaaga cacgtttagt attgcctagt ttgtatggct tgccatcata
     8401 gttttcaata atttgcggtt taacatttga agcattgaaa tcaggtgatg tatcaacatg
     8461 agccaaaaaa ccaacagttg ggacgtcggc atcgatgtta ctttctaatg tagcaaataa
     8521 gtagccattt tcatctaaat cagttggcaa tcctaattgt tgtaattctt tttctaataa
     8581 atgtaacaaa tcccattgct tttcagttga aggtgttgtt gtagattttg gatcagattg
     8641 cgtatcaatt gtcgtatatc ttgttaatct atctatcaat tggttcttca ttatattcga
     8701 ccccttaaac tctattattc atgttgtaag attttttata tgtcttacct ttgattttac
     8761 catacagttg tttgatacgt gtgtacaggt aatatagaat ttcagaaact aatataccga
     8821 aagcaatcgc acctgaaatc agtgtaactt ctaaaaatgt atttacagca cttgtataat
     8881 cattcgatac taaaaaacga gtcgcttgat aagctgcacc accaggtact aatggtataa
     8941 tgcctggcac tatgaatata attaccggtc gtttataact gcgactcata gtatgactca
     9001 ttaagcctaa aattaagctt cccaaaaatg aagcgccaac ttttccaaac tctaaatcta
     9061 ccgttaattg gtaaatcgtc catgcaatgg cacctacaaa tccacatgct actaagagac
     9121 gtttgggtgc attgaaaatg atagagaaaa gtactgttga tataaagctg attgtaaaat
     9181 gaaataaata aaatagcatg ctttaacagt ccttccttaa atgattaata aaacgattgc
     9241 gacaccagca ccgattgcga atgctgttaa tgcagcttca acaccacgag acatacctgc
     9301 aagtaattca cccgctaata aatctcgaat ggcattggta attaatatac cagggacaag
     9361 tggcatgaca ctggctatag taatgatatc ttgattggtt gcaataccta atttagtaaa
     9421 tgtggctgca atggatatga ccacagcggc tgcaacaaac tctgagaaaa atttaatttg
     9481 tatatagcgt tgcacaaagc tgaatgttaa aaatgcggat ccaccagcaa tgactgcaat
     9541 ccaacaatct gatgcgacac caccaaacat gaacaggaag aagccacatg caatggcagc
     9601 tgcaaagaaa ttcgttaaaa aagaatattg taatgatgca tgctgtaaat gaataaattc
     9661 agatttagct tcatcaattg tgagttcttt atttgatatt ttacgtgaaa gactattcgt
     9721 taaagcgatt ttctctaaat ctgttgtacg ctcttgtaca cgaattaatc ttgtacttgt
     9781 acgatcattt aatgaaaaaa taattgcagt tgaactgaca aaactatatg tattatgaag
     9841 accataacta tgtgcgatac ggttcattgt atcttcaact cgatatgttt cagcacctga
     9901 ttcaagtaaa attctacctg caattaatac aacatcaatc actttgtttt catctataat
     9961 tgtgattgaa tctggcatat caattcacct ccaatgatat gtgttattta tttgaacaat
    10021 tgaagtttac aacttgttgt tgcaactttc aatagtgaga ctttgtgtta gtatgatgaa
    10081 cttgtatggt tcaaatttaa ataagaaaaa ctgttaatct ttgctattat actatgattt
    10141 aataatagca aaggattaac agttttgtcg ttgttataga ttgatgatag ggttaaacat
    10201 tactttattt cggccttgat ttttggctac atgcaccata tcgtctgcat ctttaaacac
    10261 tttacgctgt gattttggat cgtcatctgt taaataacca acaccgatag acactgacaa
    10321 tttaataact tctttgtttg gtaaatggaa tgatgatttt tcaacacccg aacgaatatt
    10381 ttcagctaat ttaacacttt gatcaagtga atagttgtga atgacaactg agaactcttc
    10441 gccaccattt ctaaaaattt taaattgatt cggcacatag tttttaagta attgagacat
    10501 ttgttttaat acagcatcac ctgatttgtg tgagtaggta tcattgacat ctttaaatcc
    10561 atcgatatcg attaataata atgcgatact ttgatgttct ttttcagctt ttcgtgaaat
    10621 ttcatttaaa tgtctatcaa attcttttac attacctaag cctgttaagt aatcatattt
    10681 atcttcgttt tcataacgat ttacgagtga gaagaaatgc caaatatcga caaatgttat
    10741 cgctgaagct aaagtgataa ttaatgaaat tggtattaaa atgataactt ccgatagtgt
    10801 gtaaatagga ctcactaatg cgacaccaaa taaaatgatt attgtaacaa cattaagtat
    10861 taacaatgat agcacatcat tttgttttaa aaatggtcca atagcacttg ttactgcggc
    10921 aataacaatc aacgtaacac cgtacataat cgagttgtta aatactacaa tttcaacaat
    10981 tgctacaatt actgtggcag ataatgtata gaccatattt gtaaatctac ctaaaaacaa
    11041 taaaggaacg aatgttaagt gaattaaata atcttcacga taagggatag ggtagacaga
    11101 taataataat gatacgattg tcattaatac agtgacatat gccttagaaa aaaccatacg
    11161 tttgttttct gaatactgta agcgatggaa taaatagatt ccagcgacta taacagatat
    11221 attgtatata aatgcttcga acatgtctga atcgactcct ttaattgacc actagctatt
    11281 gtaagtgaaa acttacaatt tgtcattagt ttacatataa aattaatgta tgatatagac
    11341 tttgatgtta aaatgttgcc ttaaatgata tgatgaaaaa atgaataata ggcgatatat
    11401 aagaaatgaa tcgtatagtt gtaaatatga tatcattgat tgagcgaatt aatttataat
    11461 aaagctataa gatatacgta gaaaatagat atatcattct ataaagacaa tattaaataa
    11521 atataacgtt aaaaacaatt aatatcgatg aaggtgaata aatggttaca ttattactag
    11581 ttgcagtaac aatgattgtc agtttgacga taacaccaat tgttattgca atatcgaaaa
    11641 gattaaattt agttgataaa ccaaatttta gaaaagtaca cactaaacct atttcagtta
    11701 tgggtggtac ggtgattctc ttttcatttt taataggtat ttggattggt catcctattg
    11761 aaacagaaat caaaccactt attattggtg cgattattat gtacgtactt gggcttgtag
    11821 atgatatcta cgatttgaaa ccgtatataa aattggctgg tcaaattgct gctgccttag
    11881 tagttgcttt ttatggtgtg actattgatt ttatttcgtt gccaatgggt acaactattc
    11941 attttggatt tcttagtatt ccaattactg tgatttggat tgttgctatt acaaatgcaa
    12001 ttaacttaat tgatggactc gatggtttgg catcgggtgt ttctgcaatc ggactcatta
    12061 caataggatt cattgcaatt ttacaagcta atattttcat aacgatgatt tgttgtgttt
    12121 tattaggctc tttaattggg tttttatttt acaatttcca tcctgccaaa atatttttag
    12181 gtgatagtgg cgctttaatg attggattta tcatcggatt cctttcttta ctcggattca
    12241 aaaatattac aattattgca ttgttcttcc caattgttat cttagcagtt ccattcattg
    12301 ataccttgtt cgcaatgatt cgacgtgtga aaaaagggca gcatataatg caagctgata
    12361 aatcgcattt gcatcataag ttattagctt taggctacac acatagacaa acggtattat
    12421 taatctattc aatttctatt ttatttagtc tttcgagcat tatcttgtat gtatcgccac
    12481 cattaggtgt tgtattaatg tttgtattaa tcatatttag tattgaatta attgttgaat
    12541 ttacaggatt aatagataac aactaccgac caatattaaa tttaattagt cgtaagtcat
    12601 ctcataaaga ggaatagatt ttaaaacggt aagtaataaa ataaaaaatg atgtctgtac
    12661 ttttatgttg ttataaagaa ggcatactca caaaaaacac tagcatggaa cgtgctttcc
    12721 aaattgcaaa gtaacaatac gcaattagag ttagccatcc taagctagtg ctttttattt
    12781 ttattcagtt ggtatatcga aaggtaactg ctttggagtt tcttcagtca aatcgaaatt
    12841 tccagcagtc atttgattta aaaaattaat aaacgcttca tagtcacttt taacgacatc
    12901 gatatagtag cttaccttat cagtgtaagt ttggtttctt aacataaaat gagttgaggc
    12961 taattcatat tcaaatttac cagtttgatc ataattcagt gttactatac atggtactgc
    13021 ttctcgtagt tcgacacgtc cgatatcata aatgacgtct ctgacagcac cgctataggc
    13081 gcgaattaaa ccgccaccac ctaatttaat accaccaaaa tatcttgtta ctacgacaca
    13141 cgcattatga acatcgagct tttttaatat gtctaacatt gggacaccgg cagttcctgt
    13201 cggttcacca tcatcattcg ctttttgaat attcatttca ggtccaatag tatatgcaga
    13261 acaattatga gtggcatctt tatgttcttt ttttattgca gcaataaatg ctttagcttc
    13321 atcttcattt tgaacaggtt tgatatgagc aatgaatctt gatttactaa tcacattttc
    13381 aataatgtgt tcttttttaa cagtaatgat attttgtgtc ataataactc cttaattcat
    13441 aagcttaaga ttatttaatc ttcattatac actgaaaatg atatgactat aaatcgtttg
    13501 attgccattt tctttttaac tgaaatattg tatcattgct atgagtatat tttaggagga
    13561 cgactatgaa aattgctgtg atgaccgatt ctacaagtta tctgtcgcag gacttaatcg
    13621 ataaatataa tattcaaata gcgccattaa gtgtgacttt tgatgatggg aagaacttta
    13681 cagaaagtaa tgaaatagca attgaagaat tttataataa aatggcatcg tctcaaacga
    13741 ttccaacaac aagccaacca gcaattggcg aatggattac taaatatgaa atgttaagag
    13801 atcaaggtta cacagatatc attgtcattt gcttatcaag tggaattagt ggaagttatc
    13861 aatctagtta tcaagcaggg gaaatggttg aaggtgttaa tgtacatgca tttgatagta
    13921 agcttgcagc aatgattgaa ggatgttatg tattacgtgc tattgaaatg gttgaagaag
    13981 gatacgaacc acagcaaatt attgatgatt taactaatat gcgtgaacac acaggtgcat
    14041 atttaattgt tgatgactta aagaatttac aaaaaagtgg tcgaattact ggtgctcaag
    14101 catgggttgg aacattattg aaaatgaagc cagttcttaa gtttgaagat ggcaagatta
    14161 taccagaaga aaaagttcgt actaaaaagc gtgccattca aacattagaa aagaaagtat
    14221 tagatattgt aaaagacttt gaagaagtaa ctttatttgt cataaatgga gaccatttcg
    14281 aagatggaca agcgttatac aaaaagttac aagatgattg tccttcagct tatcaagtag
    14341 catactctga gtttggtcca gttgttgcag cacatttagg ttcaggtgga ttaggtttag
    14401 gctatgttgg cagaaaaata agattaacat aattataaaa ttttaataaa agagtctata
    14461 ttgtaattgg aaattatctc tcgtatacat ggctttaaat gttcatcatt tgaatgtcaa
    14521 aatgctaaag atataagaaa atcattataa tattagactc ttttttacgt tgaaatgagg
    14581 ttttaagcat taaacattac gggaaattaa ttcatcctca tacttcactt actaatgaaa
    14641 aaattaataa agaagtaaca ggtgtcatca aacaaaagtc aaactattat tgtgttcaat
    14701 gtgaaagtac aaatccaaag catttttatc agtatgattc ctcagtacat tccaagaaaa
    14761 ttgtatattg cagaaattgt atatcactgg gtcgaatgga taatgtaaca agatataaaa
    14821 taacagaaag ttcgcaaagt tcatcacaag catattatca tctctcattt gaattatcgg
    14881 aacaacagtc ttatgcctca gaacatattg ttcgagccat tagaatgaga caaacgattt
    14941 tgttatatgc agtaacaggt gcaggtaaga cagaaatgat gtttcaaggc attcaatatg
    15001 caagacgaca gggagataat atagctattg tgtcaccacg tgtagatgtt gttgtagaaa
    15061 ttagtaaacg tattaaagac gcatttctta atgaagatat agacatacta caccagcaat
    15121 caagtcaaca atttgaaggg cattttgttg tatgcacagt gcatcaactt taccgattca
    15181 aacagcactt tgatactatt tttattgatg aagtcgacgc ctttccttta tcaatggata
    15241 aaagtttaca acaagcattg aagtcatctt ctaaagttga acatgcaacg atttatatga
    15301 cagcaacacc accgaaacaa cttctgtcag agattcccct cgaaaatata attaaattgc
    15361 cagctcgctt tcataaaaaa tcacttccag ttcctaaata tcgctatttc aaacttaaca
    15421 ataataagat tcagaaaatg ttatatcgaa ttttacaaga tcaaattaat aatcaacgtt
    15481 atacactggt gttttttaac aatatagaaa caatgattaa aacattttcg atttataagc
    15541 agaaaattac taaattaaca tatgtccata gcgaggatgt ttttcgcttt gaaaaagttg
    15601 aacaattaag gaatggacaa tacgatgtca tttttactac gacaatatta gaacgtggat
    15661 ttacaatggc aaatttagat gttgttgtta tcgatgcaca tcaatatact caagaggctt
    15721 taatacaaat tgctggacgt gttggacgaa aattagaatg tcctactgga aaagtattgt
    15781 ttttcatgaa ggtgtaagta tgaatatgat tcaagctaaa aaagagattc aaaagatgaa
    15841 caaattagca ttaaaaagag gttggattga tgaataattg tttgagttgt ggtgctaagt
    15901 tatatgaaaa tataaccatt tataatttgt tcaagaaacc taatagatta tgtgacagat
    15961 gcaaagagaa ttgggacaat attaaacttg atattaaagc aaggcgatgt tcaaggtgct
    16021 taaaatactt agatcaaaat gaagcgtatt gtttagactg caagtttcta tcggcacact
    16081 ttaatttaat ggaacaatta tattgtcaat ttcaatatga cggtttaatg aaagagatga
    16141 tacatcagta taaattttcg aaagactatt atttatgtga attattggca catttgattg
    16201 aaataccaca aacatcttat gactatattg tgccaattcc ttcttcgccg gcacatgatt
    16261 tatctagaac atttaacccg gtagaagcag tactaaaagc aaaagggatt cgctttgata
    16321 agattttaaa gatgtcaaat agaccaaaac agtctcattt aactaagaaa gagcgtctgg
    16381 cagatgaaaa tccatttatt attgatacgg aattagattt aaatggtaag gaaatattac
    16441 tcgttgacga tatttataca actggattaa caattcatcg tgcagggtgt aaattatatg
    16501 ctaaaaatat cagaaaattc aaagtgtttg cgtttgcacg atagcgtaaa aatgttaaaa
    16561 tataataaag agttaccaat aaagaggttt aaggagagat tactatgatt agatttgaaa
    16621 ttcatggaga taacctcact atcacagatg ctattcgcaa ctatattgag gaaaaaattg
    16681 gtaagttgga acgttatttt aatgacgtac caaatgcagt ggcgcatgtt aaagttaaaa
    16741 cttattcaaa ttcagctact aaaattgaag taacaattcc attgaaaaat gttacgttaa
    16801 gagctgaaga gcgaaacgat gatttatacg caggtattga tttaattaat aataaacttg
    16861 aaagacaagt tcgaaaatat aaaacacgta ttaatcgtaa gagccgtgat cgaggagatc
    16921 aagaagtgtt tgttgccgaa ttacaagaaa tgcaagaaac acaagttgat aatgacgctt
    16981 acgatgataa cgagatagaa attattcgtt caaaagaatt cagcttaaaa ccaatggatt
    17041 cagaagaagc ggtattacaa atgaatctat taggtcatga cttctttgta ttcacagaca
    17101 gagaaactga tggaacaagt atcgtttacc gccgtaaaga cggtaaatat ggcttgattc
    17161 aaactagtga acaataaatt aagtttaaag cacttgtgtt tttgcacaag tgctttttta
    17221 tactctaaaa gcaatttcta acaatttcat agttcgataa tgtaatttgt tgaatgaaac
    17281 atagtgacta tgctaatgtt aatggatgta tatatttgaa tgttaagtta ataatagtat
    17341 gtcagtctat tgtatagtcc gagtcgaaaa tcgtaaaata tttataatat aatttattaa
    17401 gaagtataat tgcgttttga gaatatattt attagtgata aacttgttga caacagaatg
    17461 tgaatgaagt atgtcataaa tatatttata ttgattctac aaatgagtaa ataagtataa
    17521 ttttctaact ataaatgata agatatattg ttgtaggcca aacagttttt tagctaaagg
    17581 agcgaacgaa atgggatttt tatcaaaaat tcttgatggc aataataaag aaattaaaca
    17641 gttaggtaaa cttgctgata aagtaatcgc tttagaagaa aaaacggcaa ttttaactga
    17701 tgaagaaatt cgtaataaaa cgaaacaatt ccaaacagaa ttagctgaca ttgataatgt
    17761 caaaaagcaa aatgattatt tagataaaat tttaccagaa gcatatgcac ttgttaggga
    17821 aggctctaaa cgtgtattca atatgacacc atataaagtt caaattatgg gtggtattgc
    17881 aattcataaa ggtgatatcg ctgagatgag aacaggtgaa ggtaaaacgt taacagcgac
    17941 aatgccaaca tacttaaatg cattagctgg tagaggtgtt cacgttatta cagtcaatga
    18001 atacttatca agtgttcaaa gtgaagaaat ggctgagtta tataacttct taggtttgac
    18061 tgtcggatta aacttaaaca gtaagacgac tgaagaaaaa cgtgaagcat atgcgcaaga
    18121 cattacttac agtactaaca atgagttagg ttttgactat ttaagagata acatggtgaa
    18181 ttattctgaa gatagagtaa tgcgtccatt acatttcgca atcattgatg aagttgactc
    18241 aattttaatt gatgaggcac gtacgccatt aattatttct ggtgaagctg aaaagtcaac
    18301 gtcactttat acacaagcaa atgtttttgc aaaaatgtta aaacaggacg aagattataa
    18361 atatgatgaa aaaacaaaat cagtacattt aacagaacaa ggtgcggata aagctgaacg
    18421 tatgttcaaa gttgaaaact tatatgatgt acaaaatgtt gatgttatta gtcatatcaa
    18481 cacagcttta cgtgcgcacg ttacattaca acgtgatgta gactatatgg ttgttgatgg
    18541 cgaagtatta attgtcgatc aatttacagg acgtacaatg ccaggacgtc gtttctcgga
    18601 aggtttacac caagctattg aagcgaagga aggcgttcaa attcaaaatg aatctaaaac
    18661 tatggcgtct attacattcc aaaactattt cagaatgtac aataaacttg cgggtatgac
    18721 aggtacagct aaaactgaag aagaagaatt tagaaatatt tataacatga cagtaactca
    18781 aattccgaca aataaacctg tgcaacgtaa cgataagtct gatttaattt acattagcca
    18841 aaaaggtaaa tttgatgcag tagtagaaga tgttgtagaa aaacacaagg cagggcaacc
    18901 agtgctatta ggtactgttg cagttgagac ttcagaatat atttcaaatt tacttaaaaa
    18961 acgcggtatc cgtcatgatg tgttaaatgc gaaaaatcat gaacgtgaag ctgaaattgt
    19021 tgcaggcgct ggacaaaaag gtgccgttac tattgccact aacatggctg gtcgtggtac
    19081 agatattaaa ttaggtgaag gcgtagagga attaggcggt ttagcggtaa taggtacaga
    19141 acgacatgaa tctcgtcgta ttgatgacca gttacgtggt cgttctggac gtcaaggtga
    19201 taaaggggat agtcgcttct atttatcatt acaagatgaa ttaatgattc gttttggttc
    19261 tgaacgttta cagaaaatga tgagccgact aggtttagat gactctacac caattgaatc
    19321 aaaaatggta tcaagagctg tagaatcagc acaaaaacgt gtagaaggta ataacttcga
    19381 cgcgcgtaaa cgtatcttag agtacgatga agtattacgt aaacaacgtg aaattatcta
    19441 taacgaaaga aatagtatta ttgatgaaga agacagctct caagttgcag atgcaatgct
    19501 acgttcaacg ttacaacgta gtatcaatta ctatataaat acagcagatg acgagcctga
    19561 atatcaacca ttcatcgact acattaatga cattttctta caagaaggtg acattacaga
    19621 ggatgatatc aaaggtaaag atgctgaaga tattttcgaa gtcgtttggg ctaagattga
    19681 agcagcatat caaagtcaaa aagatatctt agaagaacaa atgaatgagt ttgagcgtat
    19741 gattttactt cgttctattg atagccattg gactgatcat atcgacacaa tggatcaatt
    19801 acgtcaaggt attcacttac gttcttatgc acaacaaaat ccattacgag actatcaaaa
    19861 tgaaggtcat gaattatttg atatcatgat gcaaaatatc gaagaagata cttgtaaatt
    19921 cattttaaaa tctgtagtac aagttgaaga taatattgaa cgtgaaaaaa caactgagtt
    19981 tggtgaagcg aagcacgttt cagctgaaga tggtaaagaa aaagtgaaac cgaaaccaat
    20041 cgttaaaggc gatcaagttg gtcgtaacga tgattgtcca tgtggtagtg gtaaaaaatt
    20101 caaaaattgc catggaaaat aaatgatata aaataactcc ttccaattaa acacctatag
    20161 tttgtgttat gggaggagtc tttttatttt acaagcgtta aatactttaa aaaatgtgaa
    20221 gaagttgtta aacgttgtta tgtacttagt ttaaaaaaat aggtttaagc atatgtctat
    20281 gataaatgta ctgtttttta acaataaaag tataaactaa ttgtcagtgg gcttatattt
    20341 cttaacattg ttatttaaca aaattatgtt aaaatttagc attataaaag atataaatca
    20401 atgacttgaa ttgaattata aataggagcg aaggctatgg aattatcaga aatcaaacga
    20461 aatatagata agtataatca agatttaaca caaattaggg ggtctctttg acttagagaa
    20521 caaagaaact aatattcaag aatatgaaga aatgatggca gaacctaatt tttgggataa
    20581 ccaaacaaaa gcgcaagata ttatagataa aaataatgcg ttaaaagcaa tagttaatgg
    20641 ttataaaaca ctacaagcag aagtagatga catggatgct acttgggatt tattacaaga
    20701 agaatttgat ggagaaatga aagaagactt agagcaagag gtcattaatt ttaaggctaa
    20761 agtggatgaa tacgaattgc aattattatt agatggacct cacgatgcca ataacgcaat
    20821 tctagagtta catcctggtg caggtggcac ggaatctcaa gattgggcta atatgctatt
    20881 tagaatgtat caacgttatt gtgagaagaa aggttttaaa gttgaaactg ttgattattt
    20941 acctggagat gaagcaggga ttaaaagtgt aacattgctc atcaaagggc ataatgctta
    21001 tggttattta aaagctgaaa aaggtgtaca ccgactagta cgaatttctc catttgattc
    21061 atcaggacgt cgtcatacat catttgcatc atgtgacgtg attccagatt ttaataatga
    21121 tgaaatagag attgaaatca atccggatga tattacagtt gatacattca gagcttctgg
    21181 tgcaggtggt cagcatatta acaaaactga atcggcaata cgaattaccc atcacccctc
    21241 aggtatagtt gttaataacc aaaatgaacg ttctcaaatt aaaaaccgtg aagcagctat
    21301 gaaaatgtta aagtctaaat tatatcaatt aaaattggaa gagcaggcac gtgaaatggc
    21361 tgaaattcgt ggcgaacaaa aagaaatcgg atggggaagc caaattagat catatgtttt
    21421 ccatccatac tcaatggtga aagatcatcg tacgaacgaa gaaacaggta aggttgatgc
    21481 agtgatggat ggagacattg gaccatttat cgaatcatat ttaagacaga caatgtcgca
    21541 cgattaat
//
LOCUS       DAMZPP010000034        20523 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMZPP010000034 DAMZPP010000000
VERSION     DAMZPP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.034"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..1052
                     /locus_tag="POH73_000965"
     CDS             <1..1052
                     /locus_tag="POH73_000965"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDI6886935.1"
                     /translation="PFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKIL
                     AKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHD
                     VRYENFDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPK
                     SKADFAFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGIIRRYLIEEKNYLEAVIGLPA
                     NIFYGTSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRK
                     KTIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQ
                     EINAYLKELGVLKDE"
     gene            1045..2199
                     /locus_tag="POH73_000966"
     CDS             1045..2199
                     /locus_tag="POH73_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI6886936.1"
                     /translation="MSNTQTKNVPELRFPGFEGEWEEKKLGDLIKVNSGKDYKHLEKG
                     DIPVYGTGGYMTSVSEPLSEIDAVGIGRKGTINKPYLLEAPFWTVDTLFYCTPKKETD
                     ILFILSLFRKINWKVYDESTGVPSLSKQTINKINRFVPSNKEQQKIGEFFIKLDRQIE
                     LEEQKLELLQQQKKGYMQKIFSQELRFKDENGNDYPNWEEKKIEDIASQVYGGGTPNT
                     KIKEFWNGDIPWIQSSDVKVNDLILRQCNKFISKNSIELSSAKLIPANSIAIVTRVGV
                     GKLCLVEFDYATSQDFLSLSSLKYDKLYSLYSLLYTMKKISANLQGTSIKGITKKELL
                     DSIIKIPHNLEEQQKIGDLFYKIDKYISFNKCKIEILKSLKQGLLQKIFI"
     gene            2236..4053
                     /locus_tag="POH73_000967"
     CDS             2236..4053
                     /locus_tag="POH73_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HDI6886937.1"
                     /translation="MEKLNEAIKNYIDSDELFALFIDGQWGVGKTYYIKNKILKEFDD
                     KIEIRYISLYGLQNLYEIKSIIISKLINTSTKNLRKVLKSMKNFQVVPYLNMNNFSDN
                     ILRYIDDNSLKKIKNSLMKNNKSALLIIDDVERMSDKVSFEEFLGFIRNDLIDYLDCK
                     VLFIGNLDELNKKADFHKNSEKIISRILKFPNNREVAMDIVKSNLPKLFVKNISTKEL
                     FNGFFDISDEKKEIIFEKSKQNNLNYKYYHLNMRTLNLVVSNFKLIINKMQDEIKYKS
                     KDFKITLYISLFISLFILYNEFRSGNLGESEIKDLQFTYNDFNNLNTKTSIALFKYLY
                     KNNELANYVFFDIELKNLLLYGIFDESIFLKNITNNFKEEDAKADLLNVLKEFFKLSE
                     SELLEKEIQAIEIIKDKNNENFNYKVKAYLSLLNLKGKNLYLIDGIKISELEDELVDN
                     YDLHQNNLIVGDTINRLDLVSFSNEQLYEEIKSLKDRLINKRTTLMTSIYNQYLETIL
                     NGDFTNRTKLKEAYIGMKDIDTIEIIYNNLNKVSNELKSSNEKIINLSAFIKDNHCNN
                     DDNIQLEYIIGEMKKLKDGTKDRVTQMCFSMLIDTLKDI"
     gene            complement(4315..4614)
                     /locus_tag="POH73_000968"
     CDS             complement(4315..4614)
                     /locus_tag="POH73_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HDI6886938.1"
                     /translation="MSSKDAKKSQEEARLQQLKQEMRNSTDNMVSNAKNNVALKFQTL
                     NEIIETINNSSQSLEGTFEGKASEAAQQSINKLKNNNENLKQKFETLLNTLKING"
     gene            complement(4629..6239)
                     /locus_tag="POH73_000969"
     CDS             complement(4629..6239)
                     /locus_tag="POH73_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HDI6886939.1"
                     /translation="MNLSDALSRSVSDMSYENLDKDQTYDFLGQDYKVIEKRDDTQNG
                     FRAYVFAPVIKGKVDKSQLLIGYAGTDPYSLNDIRTDLQLPIHHNTNNLKINNHYKDL
                     KNSTIHKKYNTIKDKNDLFSFSSNLNNKALFGDLWAAKFAPTQMDESDAFTESVKKKY
                     PDSEIHPLGHSLGAFLAQYNLIKHNLDNGTTFAAPNVYHSFTGDFKKDIDNGVYDSKI
                     RNIGHFDDPINNLNFFNPRIGKNITSMPHYDGFITNLPLIGQHTLKSYNDFDADGNVK
                     EMPELEQFNDDQSLNNRFQLFDPFGIKSRFFDSIKIDSSILNRTSENDFDPFAILNKK
                     DKLFESNDLDIEGFNNKQKEDLSIDLNPFDDELNDEDEPLIPKRGDSKKLKAEKKISV
                     KKTKSSSKNSGKKVIIQPDEVRNIASTIRDRLFLYTQIITALDEYREETMSGANRILD
                     KYQSELLSGSHEFITPNDLEQYMEILSVEGSSGNFKFYNSGLMDDLIQALDQNKKDLL
                     QFAEKLEFAANKFEEKDLEESDVFGLFS"
     gene            6282..6731
                     /locus_tag="POH73_000970"
     CDS             6282..6731
                     /locus_tag="POH73_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6886940.1"
                     /translation="MIRKTKVIILIIILIIAILMMFAYMSHKQKKEHYIAIQEKRIDL
                     FFKHNLNNYKNMKVTDFHKTPMGGYFIVGYINDDKKYKFQASIDSGSNNQYQKNIGYH
                     DDKLGKLFKEKDPKYKLSVDEIIEKEHLDKNEYEADPPLFFFSGPIE"
     gene            6941..7318
                     /locus_tag="POH73_000971"
     CDS             6941..7318
                     /locus_tag="POH73_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6886941.1"
                     /translation="MNKKSLMIVIIILILILGGIFLKMKYDEKKYYDEQKERITIYMK
                     HNVKGYKNISFTNCKENPMDGYSISGYINNDRKLSFTAGIRSIDDFQFDTDISYTDEL
                     GRKFIKNPKSVSEIKKELNTSNK"
     gene            complement(7444..9864)
                     /locus_tag="POH73_000972"
     CDS             complement(7444..9864)
                     /locus_tag="POH73_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HDI6886942.1"
                     /translation="MTNKMKKWQKLSTITLLMTGVIALNNGEFRNVDKHQIAVADTNV
                     QTPDYEKLKKTWLDVNYGYDQYDENNQDMKKKFDAKEKEAKKLLDDMKTDTNRTYLWS
                     GAENLETNSSHMTKTYRNIEKIAESMQHKNTVLKTVENKLKIKEALDWMHKNVYGKNP
                     SQKVEDLTKNRKGQTTPKNNSLNWWDYEIGTPRALTNTLLLMDDMLTKDEMKNYSKPI
                     STYAPSSDKILSSVGESEDAKGGNLVDISKVKLLESVIEEDVDMLKKSIDSFNKVFTY
                     VQDSATGKGRNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKESPFKTTQDNA
                     TLSNWIDEGFMPLIYKGEMMDLSRGRAISRENETSHTASATVMKSLLRLNDTMDDSTK
                     TRYKQIVKTSVNSDSSYNQNNYLNSYSDIAKMKKLMNDSTISKNDLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNIARYENINGENLKGWHTGAGMSYLYNSDVKHYRDNFWA
                     TADMTCLPGTTTLNDMPSTNTKNDKSFVGGTKLNNKYASIGMDFENQDKTLTAKKSYF
                     ILNDKIVFLGTGIKSTDSSKNPVTSVENRKANGYKLFKDDIEITTSDVNAQETHSVFL
                     ESNDTKKNIGYHFLDKPKITVKKESHTGKWSEINKSQKKDDKKDEYYEVTQTHNTSDS
                     KYAYVLYPGLSKSDFKSKNNNVSIVKQDEDFHVIKDNDGVFAGVNYSDNTKSFDINGI
                     TVELKEKGMFVIKKKDDKAYKCSFYNPETTNTASNIESKIFIKGYTITNKSVINSNDA
                     GVNFELTK"
     gene            10389..10831
                     /locus_tag="POH73_000973"
                     /pseudo
     CDS             10389..10831
                     /locus_tag="POH73_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
     gene            10831..11274
                     /locus_tag="POH73_000974"
     CDS             10831..11274
                     /locus_tag="POH73_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6886943.1"
                     /translation="MKKKYILILLIVILIGLLVIAYTHNKKTKDHYIETQEKRINLYF
                     KYNLNNYSSMKVTSFKKTPMGGYIVDGYVNHNKNYDFKVLISVTDNHQFEDSIGYDDK
                     TFGKLFKEKDHKNELKPKDIIKKEHLDKSEYEAEPPLFFFSGRLE"
     gene            11274..11717
                     /locus_tag="POH73_000975"
     CDS             11274..11717
                     /locus_tag="POH73_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6886944.1"
                     /translation="MNKKYILIIVSVILIGMIVISYAHNKQKKDHYIETQEKRIDLFF
                     KYNLNHYKSLKVTRFKKSPMDSYFIGGYINNNKDYEFQAFINVGGRKQFQRDIGYDEE
                     KLGKLLKEQEVKTWKTVDQIIKNEHLDKSEYEAEPPLFFFSGSLD"
     gene            complement(11892..11993)
                     /locus_tag="POH73_000976"
     CDS             complement(11892..11993)
                     /locus_tag="POH73_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886945.1"
                     /translation="MQGFKEKHQELKSALCQIGLMRSISEVRQLNIA"
     gene            12500..12946
                     /locus_tag="POH73_000977"
     CDS             12500..12946
                     /locus_tag="POH73_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6886946.1"
                     /translation="MKKRYVFVVVVALLIVLIVIAYAFHKSKKEHYIETQEKRIDLYF
                     KHNLNHYKSMKITKFQKSPVNAYFIKGYINNDKQYKFQAYINTGDEGNQFNSSIGYKE
                     EKIGRLLKEKDAKDRLTVDEIIEKEHLDKNEYEAEPPLFFFSGSLD"
     gene            13139..13708
                     /locus_tag="POH73_000978"
     CDS             13139..13708
                     /locus_tag="POH73_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDI6886947.1"
                     /translation="MTAIDKVYYEDVLEDDILNLLKPYIQGKEKELYITSDNFAIDLN
                     KVTALLDLKIQYEDLDDDVSGYIDERTIYVNKNHSSNRKRFTIAHEIGHFLHNHKNKI
                     KYRNNSYAYDTEEMIEERVANDFSANLLMPKKQMEALVYKFYKENNINLTSGLSISER
                     EELINYIAIKLEVSKIAAGIRLYNLGLDK"
     gene            13708..15075
                     /locus_tag="POH73_000979"
     CDS             13708..15075
                     /locus_tag="POH73_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HDI6886948.1"
                     /translation="MNTKYLYLNFDKIYEEKDFFNVLHVDINLKISEIKESNEVLYSI
                     DSITCKKLNHYDPKLESYRDSIYLLNERLNNYNFNGKKEWKLFYLYKELIQTFEILYD
                     DTSTTNYYRGQANDWPMKAGLLRNDIIDDLKKEFENIYEDMAYKYPDLIEYTCLNKKE
                     YKAEDFKKRENNMAYLQHYGLRTTLIDITENPFIALLFLTSNSQVFNNATLDMYNINP
                     KIHSEQNLFSRVKMISKNKRIIAQKGAFFNFEKLLIFQNEQNVNRDKINKIPLVRLKL
                     NFSYDYKEKLKRELNQTQSAFQKLKITREEKLKNHKSRIKEDLKRIRNLTMKTEHDMD
                     SEKSNDYKEELEYLIKRILKDESVIKIDKEMEDLKKKKLYLDARLKKEEILTSEYLRP
                     EICKELREKLKQYHYVESELFPDVYRHIGYIQSNFLSNQTNNRTINKNNISENLVDLL
                     KLKEN"
     gene            complement(15224..15796)
                     /locus_tag="POH73_000980"
     CDS             complement(15224..15796)
                     /locus_tag="POH73_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886949.1"
                     /translation="MEKNEYTAKYNEYSQLLDATYSQAVAYLLNKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISEFKYSYNYQKKENL
                     VYCDLIEHLILHAIITKESNGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARA
                     YLPGILVEKLLIKIDDMLKGIEIYDFLESR"
     gene            complement(15894..16238)
                     /locus_tag="POH73_000981"
     CDS             complement(15894..16238)
                     /locus_tag="POH73_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HDI6886950.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIDNAEHTLFTPYM
                     METLSTLGVKPDIIDLIHKGTELEDFSAFNLSIDDTVTVCLQRTEELLKQYKNVEFND
                     KILINWRIMQEK"
     gene            complement(16279..16905)
                     /locus_tag="POH73_000982"
     CDS             complement(16279..16905)
                     /locus_tag="POH73_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886951.1"
                     /translation="MKFKAIVAITLSLSLLTACGANQHKENSSKSNDTNKKTQQTDNT
                     TQSNTEKQMTPQEAEDIVRNDYKARGANENQTLNYKTNLERSNEHEYYVEHLVRDAVG
                     TPLKRCAIVNRHNGTIINIFDDMSEKDKEEFEAFKKRSPKYNPGMNDQAEMDNESEDI
                     QHHDIDNNKAIQNDLPDQKVDDKNDKNAVNKEEKHDNRENNSAETKVK"
     gene            complement(16981..17976)
                     /locus_tag="POH73_000983"
     CDS             complement(16981..17976)
                     /locus_tag="POH73_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDI6886952.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDNSSVEKSESNEETAPVEPMPHSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            complement(18057..18707)
                     /locus_tag="POH73_000984"
     CDS             complement(18057..18707)
                     /locus_tag="POH73_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HDI6886953.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSAKNSERRGMLKGCGGCLISF
                     IILIILLSACSMMFSNNDNSTSNQSSKTQLTQKDEDKSENMPEEKSESETDKDLQSTE
                     EVPANENTENNQHEIDEITTTDQSDDEINTPNVAEEESQDDLKDDLKEKQQPSDHHQS
                     TQPKTSPSTETNKQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            19009..19464
                     /locus_tag="POH73_000985"
     CDS             19009..19464
                     /locus_tag="POH73_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HDI6886954.1"
                     /translation="MKICLMLYLLIFLSSCGTNNKIVGQRFEIQQHNEIIGRIYFSAD
                     YAHIRGIEKGTVKYFVDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IENLDDELFHLSALQDMNHRPIDIADDVATMDKSPHQNDRITLKLNKNN"
     gene            19623..>20523
                     /gene="menE"
                     /locus_tag="POH73_000986"
     CDS             19623..>20523
                     /gene="menE"
                     /locus_tag="POH73_000986"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDI6886955.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMRSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGTDITTNGLLDNTMGIQYDTSNETVVPKDSPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYDLQK
                     ILLGGAKLSASMIETALQYNLPIY"
BASE COUNT         7391 a         2770 c         3220 g         7142 t
ORIGIN      
        1 taccattcgt tcacagtgat atggaaattg atatgttagg tgatgcatat gaattcctta
       61 tcgggcgctt tgcggcgaca gcgggtaaaa aagctggcga gttctataca ccacaacaag
      121 tatctaagat actggcaaag attgtcacag acggtaaaga taaattacgt cacgtgtatg
      181 acccaacatg tggttcaggt tcattactgt tgcgtgttgg taaagagaca caagtatatc
      241 gttatttcgg acaagaacgt aacaatacca catacaactt agcacgcatg aacatgttgt
      301 tacatgatgt acgttatgag aactttgata tccgtaatga tgatacgttg gaaaatccag
      361 cctttttagg caatacattt gatgcggtta ttgcgaaccc accatacagt gcgaaatgga
      421 cagcagattc aaaatttgaa aatgatgaac gttttagtgg ttacggcaaa cttgcgccaa
      481 aatccaaagc agactttgcc tttattcaac acatggtaca ttacttagac gatgaaggta
      541 ccatggccgt tgtactccca catggtgtct tattccgtgg tgccgcagaa ggtatcattc
      601 gtcgttattt aattgaagaa aagaactact tagaagccgt gattggtttg ccagcgaata
      661 ttttctatgg gacaagtatt ccaacatgta ttttagtatt taaaaaatgt cgccaacaag
      721 acgacaacgt attatttatc gatgcatcca atgattttga aaaaggaaaa aatcaaaacc
      781 atttaagcga tgcccaagtc gaacgtatta tagacacata taagcgtaaa aaaacgattg
      841 ataaatatag ctacagcgcg acattacaag agattgccga taacgattac aacttaaaca
      901 taccgcgata tgtcgataca ttcgaagaag aagcgccaat tgatttagat caagtccaac
      961 aagatttgaa aaatatcgat aaagaaatcg cagaaattga acaagaaatc aatgcatacc
     1021 tgaaagaact tggggtgttg aaagatgagt aatacacaaa cgaaaaatgt gccagagttg
     1081 agattcccag ggtttgaagg cgaatgggaa gagaaaaagt taggggatct tataaaagtt
     1141 aattctggaa aagattataa acatttggaa aaaggtgata taccagtcta tggtactggc
     1201 ggttatatga caagtgtttc agaaccacta agtgaaattg atgctgttgg tattgggaga
     1261 aaagggacta taaacaaacc atatttgctt gaggcgccgt tttggacggt ggatacatta
     1321 ttttattgta cacctaaaaa agaaacagac atactattta tattaagttt atttagaaaa
     1381 ataaattgga aagtatacga tgaatcaaca ggtgtgccaa gcttaagtaa acaaaccatt
     1441 aataaaataa atagatttgt cccttcaaat aaagagcagc aaaaaatagg cgaattcttc
     1501 atcaaactcg accgacaaat tgaattagaa gaacaaaaac ttgaattact tcaacaacag
     1561 aaaaaaggct atatgcagaa aatcttctca caggaattgc gattcaagga tgagaatgga
     1621 aacgattatc cgaattggga agagaagaaa atagaagata tagcaagcca agtatatgga
     1681 ggcggaacac caaatacaaa gattaaagaa ttttggaatg gagatattcc atggattcaa
     1741 agctctgacg taaaagtaaa tgatttgatt ctacgacaat gtaataaatt tatttccaag
     1801 aattcaattg agctttcttc tgcaaaactt attcctgcca attcaattgc aatagttaca
     1861 agagtcgggg ttggaaaact gtgtttggta gaatttgatt atgctacaag tcaagatttt
     1921 ttatcattaa gtagtcttaa atatgacaaa ttatactcat tatattcatt gctatataca
     1981 atgaaaaaaa ttagcgctaa tctacaagga acttcaatta aaggtataac aaaaaaagag
     2041 ttgttagata gtataataaa gataccccat aatctagaag aacagcaaaa aataggtgat
     2101 ctattttata aaattgataa atatatcagt tttaataaat gtaaaattga gatacttaaa
     2161 agtctcaaac aaggattact tcaaaaaatt tttatataaa tgatatagaa tttaaaattt
     2221 tgtagaggtg aattgatgga aaaattaaat gaagcaatta aaaactatat agatagtgat
     2281 gaattgttcg ccctttttat agatggacaa tggggagtag ggaaaactta ttatattaaa
     2341 aataaaatat taaaggaatt tgatgataaa atagaaatta ggtatataag tttatatggc
     2401 ctgcaaaact tatatgaaat taagagtata atcataagta aattgataaa tacatctact
     2461 aaaaatctta ggaaagtcct aaaaagcatg aaaaatttcc aagtagtacc ttatttaaat
     2521 atgaataact tttctgataa catattacgt tatattgatg ataattcatt aaaaaagata
     2581 aaaaattccc tgatgaaaaa taataaaagt gctttgctaa taatcgatga tgttgaaaga
     2641 atgagtgaca aagtaagttt tgaagaattt ttgggattta ttagaaatga tttaattgat
     2701 tatttagact gtaaagtgct atttatagga aatttagatg agctaaataa aaaagcggat
     2761 tttcataaga atagtgagaa aataatttct agaatattaa agtttcctaa taatagagaa
     2821 gtagcaatgg atatagtaaa aagtaattta cctaagttat ttgtaaagaa catctctact
     2881 aaagagctat ttaatggttt ttttgatata agtgatgaaa aaaaagaaat tattttcgaa
     2941 aaatctaaac aaaataattt aaattataaa tattaccatt tgaatatgag aactttgaat
     3001 ttagtagttt caaacttcaa gttaattatt aataaaatgc aagatgagat taaatataaa
     3061 tcaaaagatt ttaaaatcac cttgtacatt tcattattca tttcattatt cattctgtat
     3121 aatgaatttc gaagcggaaa tttaggtgaa tctgaaatta aggatcttca atttacatat
     3181 aatgatttta acaatttaaa tacgaaaaca tctattgctt tgtttaaata cttatacaaa
     3241 aataatgaac tagcaaatta tgtctttttt gatatagagc taaagaactt attattatat
     3301 ggtatttttg atgaatcaat ttttttgaaa aatataacga ataacttcaa agaagaagat
     3361 gctaaagctg atttattaaa tgtattaaaa gaattcttta aattaagtga aagtgaacta
     3421 cttgaaaaag aaattcaagc tatagaaata ataaaagata aaaacaatga aaattttaat
     3481 tataaagtaa aagcttattt atctttatta aatttaaaag gaaaaaactt atatttaata
     3541 gatggcatta aaattagcga attagaagat gaattagtgg ataattacga tttgcatcag
     3601 aataatttaa tagttggaga tacgataaat agactagatt tagttagctt ttcaaacgag
     3661 caactatatg aagaaatcaa aagtttaaaa gaccgtttaa taaataaaag aacaactttg
     3721 atgacttcta tatataacca atacttagaa actattttaa atggtgactt tacaaataga
     3781 actaaattaa aagaagcgta tattggaatg aaagatatag atactataga aataatctat
     3841 aataatttaa acaaagtatc aaatgagctt aaaagtagta atgagaaaat tattaattta
     3901 agtgcattca ttaaagataa tcattgtaat aatgatgata atattcaatt agaatatatt
     3961 ataggtgaga tgaaaaaatt aaaagatggt actaaagata gagttacaca aatgtgtttt
     4021 agcatgttga tcgatacact gaaagatata taggtgaata atagacatat taatttgttt
     4081 aataggataa atatataaag agtatgcttc tttttaaata gtgacttaat aagaatataa
     4141 ttaaattata catttaaatt ggcaaatctg tactatgata aaattgtagt ttttattaat
     4201 gatactaaaa taagttagat aagaaataaa aattaaaatc ccttcactat ttgtttagcg
     4261 aggggatttt agtaatgtat aaaaaagtac agataaatta ataatttctc aatatcatcc
     4321 atttatcttc aatgtattta atagggtctc aaatttttgt ttaagatttt cgttattgtt
     4381 tttgagttta tttatacttt gttgggctgc ttcgcttgct tttccttcaa atgtaccttc
     4441 taaactttgc gaagaattat taattgtttc aatgatttca tttaatgttt gaaattttaa
     4501 tgctacatta tttttagcat tgctaaccat attatcagta ctattacgca tttcttgctt
     4561 taattgttgt aatctagctt cttcttgact tttctttgca tcttttgagc tcatataaat
     4621 taccctcttt aactaaatag tccaaaaaca tcgctttctt ctaaatcttt ttcttcgaat
     4681 ttatttgcag caaattctag tttttcagca aattgtagca agtctttttt gttttgatct
     4741 aatgcctgaa ttaagtcatc cattaaacca ctattataaa acttgaaatt acctgaactt
     4801 ccctcaactg ataaaatctc catatactgt tcaagatcgt taggtgttat aaattcatgt
     4861 gaaccgctta gaagctctga ttgatattta tctaaaatgc gattagcccc tgacattgtt
     4921 tcttctctat attcatccaa tgcagtaatt atttgtgtat acaaaaatag tctatcacga
     4981 attgtgctag cgatattacg cacttcatca ggctgaatta ttactttttt gcctgaattt
     5041 ttggaactac ttttggtttt tttaacagag attttctttt ctgcttttaa ttttttacta
     5101 tcgcctcttt tgggaattaa tggttcgtcc tcatcattta attcatcatc aaaaggattt
     5161 aaatcaatgc ttaaatcttc tttttgttta ttattaaatc cttctatatc aaggtcattt
     5221 gattcgaata atttatcttt tttattgaga atagcaaaag gatcaaaatc attttcactt
     5281 gttctattta atatagaact atcgatttta atagagtcga aaaatctgct ttttatacca
     5341 aatgggtcaa ataattgaaa tctattatta agagattggt catcattaaa ttgttcaagt
     5401 tctggcattt ccttcacatt accatctgca tcgaaatcat tgtaagattt aagcgtgtgt
     5461 tgaccaatca aaggaaggtt agttataaat ccatcataat gtggcattga tgtaatattt
     5521 ttgccaatac gaggattaaa aaaattcaaa ttattaatag gatcatcaaa atggccaata
     5581 ttgcgtattt ttgaatcata gacaccatta tcaatgtctt ttttaaagtc tccagtaaat
     5641 gaatggtata cgttaggcgc tgcaaacgtt gtaccgttat ccagattatg ttttattaaa
     5701 ttatattgag ctaaaaacgc acctaaacta tgtcctaatg ggtgaatttc actatctgga
     5761 tatttctttt taactgattc agtaaaagca tcactttcgt ccatttgagt cggtgcaaac
     5821 ttagctgccc atagatcacc gaaaagtgct ttattattta aattagaaga aaaactaaaa
     5881 aggtcatttt tatcttttat tgtattgtat tttttgtgaa tagttgagtt ttttaaatct
     5941 ttataatgat tattaatttt tagattattt gtgttatgat gaatgggaag ttgtaaatca
     6001 gtacgaatat catttagact atatgggtca gtgccagcgt aacctattaa aagttgactc
     6061 ttatcaactt tacctttgat tactggagcg aaaacatatg ctctaaaccc attctgggta
     6121 tcgtcgcgtt tttcgatgac tttgtaatct tggccaagaa agtcgtatgt ttgatcttta
     6181 tctaaatttt cataagacat atcggaaaca cttcttgaaa gagcgtctga aagattcaaa
     6241 ataaaaactc ctttattaaa aatttaaaag tgaggtaaaa tatgattcgg aaaactaaag
     6301 ttattatttt aattattatt ttaattatag caatattaat gatgtttgca tatatgtcgc
     6361 acaagcaaaa aaaagaacat tatatcgcca ttcaagaaaa aagaattgat ttgtttttta
     6421 aacataactt aaataattat aaaaatatga aagtgacaga tttccataaa actccaatgg
     6481 gtgggtattt cattgtaggt tatataaatg atgataaaaa atataaattc caagcaagta
     6541 tagattcggg aagcaataat caatatcaga aaaatattgg ttaccatgat gataaattag
     6601 gcaaattatt taaagaaaaa gaccctaaat ataaactctc agtcgatgaa ataattgaaa
     6661 aagaacactt ggacaaaaat gaatatgaag ccgatccacc gttatttttc ttttcaggtc
     6721 caattgaata aatgttatgg atagaatgga tgaaaaatat aattatttaa attttaaagt
     6781 aaacctgatc atgatttaag attataagaa agaaaaattg actaatgtta aaaccaacag
     6841 tcatatgatt tgcaacgttt atacaatcat cgtaaaactc agtatgctta atttatttaa
     6901 aatgtttgtc aataagtcca gtgaaaagga gattgatttt ttgaataaaa aatctcttat
     6961 gattgtaatt ataattttaa tattgattct aggcggaatc tttttgaaga tgaaatacga
     7021 cgaaaagaaa tattatgatg aacaaaaaga aagaataacg atttatatga agcacaatgt
     7081 gaaaggttat aaaaatataa gctttactaa ttgtaaagaa aacccaatgg atggctattc
     7141 tattagtggt tatataaata atgatagaaa gttatcattt acagctggca taagatctat
     7201 tgatgatttt caatttgata ctgatatttc ttatacagat gaattgggta gaaaatttat
     7261 taaaaatcct aagtcagttt ctgaaataaa gaaagagcta aatacgtcca ataaataatt
     7321 gttcatattg tgatgaaaca aaaatatata aatcaataga tgactttaac tatgatataa
     7381 aatcttgcaa tgcttataaa atctcgacac tataaaaatg ttatagtgcc gagattttct
     7441 taattattta gttaattcaa agtttacacc agcatcatta gagtttatga cacttttatt
     7501 agttatggtg taacctttaa taaaaatttt tgattctata tttgaagcgg tatttgtagt
     7561 ttcaggatta tagaagctac atttatatgc tttatcatct ttctttttaa ttacaaacat
     7621 gcctttttct tttaattcaa cagtaattcc gtttatatca aaagatttag tattatcact
     7681 ataattaacc ccagcaaata cgccatcatt atcttttatc acatgaaaat cttcatcttg
     7741 tttaacaata cttacattat tattcttcga tttaaaatca ctttttgata aaccaggata
     7801 caaaacatat gcatatttac tgtcagatgt attatgtgtt tgagttactt cataatactc
     7861 atcttttttg tcatcttttt tttgactttt attaatttca ctccacttac cagtatgact
     7921 ttcttttttt acagttattt ttggcttgtc taagaaatga taaccaatgt tctttttagt
     7981 atcgttggac tctaaaaaga ctgaatgggt ttcctgagca ttaacatctg aagtggtaat
     8041 ttcaatatca tctttaaata atttataccc atttgctttg cgattttcaa cacttgtaac
     8101 tggattcttt gatgaatcag tacttttaat gccagttcct aagaagacaa ttttatcgtt
     8161 taatatgaaa tatgattttt tggcagttaa agttttgtcc tgattttcaa aatccatacc
     8221 gatgcttgcg tatttattat ttaattttgt cccgccaaca aaagatttat cattcttagt
     8281 attagtagat ggcatatcat ttaaagtagt agtgcctgga agacaagtca tatcggctgt
     8341 tgcccagaag ttatcgcgat agtgtttgac atcgctgtta tataaataag acatgcctgc
     8401 accggtgtgc caacctttta agttctctcc gttgatattt tcgtatcgtg cgatgttttt
     8461 cgatgtcata cttaaaccaa atgcaaagtc caggtcttta ttgtgatagg tgacacgatc
     8521 catgtcatta tatattttaa gttgctgtgt taaatcgttt ttagaaatag tactatcatt
     8581 cattaacttt ttcattttag ctatgtctga atatgaattt aaataattat tttggttgta
     8641 acttgaatca gaattaacag aagttttaac gatttgctta tatctagttt ttgttgaatc
     8701 atccatggta tcattcaatc tcaacaatga tttcattaca gtcgctgacg ctgtatgact
     8761 cgtttcattt tcacgactga tagctctacc tcgtgataaa tccatcattt cacctttata
     8821 gattaatggc ataaaccctt cgtcaatcca attgcttaat gtagcattat cttgtgtagt
     8881 tttaaaagga gattctttta tcatcggcat catttgagaa ataccctcta atagtacaac
     8941 accataagca ccagtgtaag ggacatcttg atgatcaatg taagagccat ctttatagaa
     9001 tccattgcga cctttaccag tggcagaatc ttgaacataa gtgaacactt tattaaaaga
     9061 atctatagac tttttcaaca tatctacatc ttcttcaata acactttcta aaagttttac
     9121 tttagaaatg tccactaaat ttccaccttt agcatcttct gattcaccaa cagaagataa
     9181 aattttgtca ctggatggtg catatgtact aataggtttt gaataatttt tcatttcatc
     9241 tttagtgagc atatcatcca ttagaagtag tgtatttgtt aatgctcttg gcgtaccaat
     9301 ttcataatcc caccaattca atgagttatt cttgggtgta gtttgcccct tacgattttt
     9361 agttaaatcc tcgacttttt gagaaggatt cttgccataa acatttttgt gcatccaatc
     9421 tagggcttct tttattttca acttgttttc aactgttttt aataccgtat tcttatgttg
     9481 cattgattct gcgattttct cgatattacg ataggttttt gtcatgtgag aagaattagt
     9541 ttcaaggttt tcagctcctg accacaaata tgttctattc gtatcagttt tcatgtcatc
     9601 aagtaacttc ttggcttctt tttctttagc atcaaacttc ttcttcatat cttgattatt
     9661 ctcatcatac tgatcataac cgtagttaac gtcgagccac gtcttcttca atttttcata
     9721 atctggcgtt tgaacattcg tatcagccac agcgatttga tgtttatcaa catttctaaa
     9781 ttcaccatta tttaaagcaa tcactccggt cattaataac gtaatggtgg ataatttttg
     9841 ccatttcttc attttatttg tcattgacat gtctcctttt ttgcgtgtgt gcaattgata
     9901 ttaggattaa ttaatgattc aactttccaa aggattggga aattcgttga tgcatggaaa
     9961 ccatcagtca ccatgagatt tatttaatat ctaaaaatgt aaagaaatta aaattggaaa
    10021 attaaatatt aataatctta tcgattatag cattttaaat aattaattta tacaatattt
    10081 aaagtaatta aaattgatgt aaagtaaaat gttagaaaag agacaaattt tgatttaagt
    10141 aataaaatga taagtgaact taaaaagata ttaatttaga ataagattgc tattttatac
    10201 ataatacgaa aagcaaatca gcgcgacttt atgtatttaa tactatgtca agcaagtaat
    10261 ttttaaaagg tattttgaaa caacgaatca tgagaggcgt tgaacataga aaaagatagc
    10321 atgtcaaagt aaattggttt ttgaaatcta aaatctataa gaaataaata ctacactatg
    10381 ggattaaaat gaaaaagaga tatgtatttg tggtagttgt tgcaatatta ataggcctaa
    10441 ttattattgc atatgcgcat aataagcaaa aaaagaccat tacattgaga cacaagaaaa
    10501 gcggatagat ttgtatttta agcataatct taacaattat aaaaatatga aggtgacaga
    10561 tttccataaa actccgatgg gtgggtattt cattataggt tatataaatg atgataaaaa
    10621 atataaattc caagcaagta tagatccggg aagcgataat caatatcaga aagatattgg
    10681 ttaccatgaa gataaattag gcaaattatt caaagaaaaa gatcctaaat ataaactctc
    10741 tgtcgatgaa ataattgaaa aagaacactt agacaaaaat gaatatgaag cggaatcacc
    10801 tttattcttc ttttcgggga gattagagtg atgaaaaaga aatatatttt aatattactt
    10861 atagtaattt taataggact gctagtcatt gcttatacgc acaataagaa aactaaagac
    10921 cattacattg agacgcaaga aaaacgaata aatttatatt ttaagtataa ccttaataat
    10981 tatagctcta tgaaagttac tagttttaaa aagacgccta tgggtggcta tattgtggat
    11041 ggatatgtta atcacaacaa aaattatgat tttaaagttc tcatctctgt aactgacaat
    11101 catcaatttg aagatagcat aggatacgat gataagacat ttggtaaatt atttaaagaa
    11161 aaagaccata aaaatgaatt aaaacctaaa gatattataa aaaaagaaca cttggataaa
    11221 agtgaatatg aagcagaacc accactgttc ttcttttcag ggagattaga gtgatgaata
    11281 agaaatatat tttaataata gttagtgtaa ttttaatagg tatgatagtt atttcatatg
    11341 cacataataa acaaaaaaaa gaccattaca ttgaaacaca agaaaagaga atagatttat
    11401 tttttaagta taacctgaat cattataagt cacttaaagt aacgagattt aaaaagtcgc
    11461 caatggacag ttattttata ggtggttata taaataacaa taaagactat gaattccaag
    11521 cttttattaa tgtaggtggt agaaagcaat ttcaaagaga tataggatat gacgaagaaa
    11581 aacttggaaa actattaaaa gagcaagaag taaaaacttg gaaaaccgtc gatcaaataa
    11641 ttaaaaatga acacttagat aaaagtgaat atgaagcgga accaccgttg tttttctttt
    11701 caggatcact ggattagatt cggttgtatg gtgtaataaa aattattatt atcctcacaa
    11761 tcattaaagg acaagagtat tttaaaaaat cattacataa catacaaatt ttggttatgg
    11821 aagttacaat catttgcgca atcgaatatt attatgttta aaactttacg ctccaaaaag
    11881 taaaaaggaa gttaagcaat gtttagttgc ctaacttccg atattgaacg catcagtcca
    11941 atttggcata gagcactctt tagttcttga tgtttctctt taaaaccttg catattttac
    12001 aaaaagaaaa attagcagta taattgagat aacgaaaata agtatttact tatacacctc
    12061 ctctctgcgt ctaaattgac gcctgagaga taggcgactc tactattata tcatctaatt
    12121 ggacaaattc tatgatagta gattttgtta atttaagaaa gaagaacatt tagttcagag
    12181 tttaagttat aaatggttag attatatgaa aatggtaagc caaggaatga aattatacgc
    12241 gagtatgatt taacatcttt gatgttagga aaaacagcat tttcatttcg ccattttcct
    12301 acattttata aacccatata taacagcgtt attttatgta tttataatta tgtcaagcaa
    12361 ataattttta aaagtatttt gaaacaacgt ttcatgaggg gcgtaaacgg aacatagaaa
    12421 aaagatagca tgtcaaagtg aattgttttt tgaaatctaa aatctataag aaataaatac
    12481 taaactatgg ggggttaaaa tgaaaaagag atatgtattt gtggtagttg ttgcattatt
    12541 aatagtacta attgttattg catacgcgtt tcacaaaagt aaaaaagaac attatattga
    12601 gacacaagaa aagcgaatcg atttatattt taagcataac ctgaatcact ataagtctat
    12661 gaaaataaca aagtttcaaa aaagccctgt gaatgcttac tttattaaag gatatataaa
    12721 taatgataaa caatataaat ttcaggcata tataaataca ggagatgaag gaaatcagtt
    12781 taatagtagc ataggatata aggaagaaaa aatagggcga ttattgaaag agaaagatgc
    12841 taaagaccga ttaacagtgg atgaaataat tgaaaaagaa cacttagaca aaaatgaata
    12901 tgaagccgag ccaccgttgt tcttcttttc gggatcgttg gattagattc ggttgtacag
    12961 tgaaaaaata ttttattatt aattttagtg attaaaacaa taaatgtaag taaagatatg
    13021 cgcatattta attagtgact aaaataatga aagttagact ggttatttta agctttagaa
    13081 tataaaatat aattaataga tagagaagat taacaatatc ttttaggagg cgatttttat
    13141 gactgccatt gacaaagttt attatgaaga tgtattagaa gatgacatac ttaatctgct
    13201 taaaccatat atacagggga aagaaaagga actttatatc acttctgata attttgcaat
    13261 tgatttaaat aaagtgaccg cattactgga tttaaaaatt cagtatgaag atttggatga
    13321 tgatgtttcg ggttatattg atgaaagaac tatttatgta aataaaaatc attctagtaa
    13381 tagaaaaaga tttacaattg cccatgaaat aggacatttt ttacacaatc ataaaaataa
    13441 aatcaaatat agaaataata gttatgcata tgacactgaa gaaatgattg aagaaagggt
    13501 ggctaatgac ttttcagcta atttactaat gcccaaaaag caaatggaag ctttagtata
    13561 taagttttac aaggaaaata atattaattt aacttctggt ttatcaattt cagaaagaga
    13621 agagttaatt aactatattg caattaagtt ggaagtttct aaaatagcag ctgggattag
    13681 actttataat ttagggttgg ataaataatg aatactaaat atttatattt aaatttcgat
    13741 aaaatttatg aagaaaaaga ttttttcaac gttttacatg tagacattaa ccttaaaata
    13801 tcagaaataa aggaaagtaa tgaagtatta tatagtattg attcaattac ttgtaagaaa
    13861 ttgaatcatt atgatccaaa attagaatca tacagagaca gtatatattt attgaatgaa
    13921 cgtctaaata attataattt taatggtaaa aaagaatgga aattatttta cttgtataaa
    13981 gaattaatcc aaacttttga aatattatat gatgatactt caaccacgaa ttattataga
    14041 ggtcaagcaa atgattggcc aatgaaagca ggattattaa gaaatgatat aatagatgat
    14101 ttaaaaaaag agtttgagaa tatatacgaa gatatggctt ataagtaccc agatttaata
    14161 gagtatacgt gcttaaataa aaaagaatat aaagcagaag attttaaaaa aagagaaaac
    14221 aatatggctt atttacaaca ttatgggcta agaactacat taattgatat tactgaaaac
    14281 ccctttatag cgctcctatt tttaacttct aactcacaag tgtttaacaa tgcaacctta
    14341 gacatgtata atatcaatcc taaaatacac agcgaacaaa atttgttttc aagagtaaaa
    14401 atgattagta aaaacaaaag aataatagct caaaagggtg cgttttttaa cttcgaaaag
    14461 ctattgattt ttcagaatga acaaaatgta aatagagata aaattaataa aatacctcta
    14521 gttcgtttaa agttaaattt ttcatatgac tataaagaaa aattaaaaag ggagttaaat
    14581 caaacgcaat ctgcttttca aaaactaaaa ataactagag aagagaaatt gaaaaatcat
    14641 aagagtagaa ttaaagaaga cctcaaaaga atacgcaatt taactatgaa aactgaacat
    14701 gacatggata gtgaaaaatc taatgattac aaagaggaat tggaatattt aattaaacga
    14761 atcttaaaag atgaaagcgt tattaaaata gataaagaaa tggaagactt aaaaaagaaa
    14821 aaactatatt tagatgcaag gttgaaaaaa gaggaaatct tgactagtga atatttaagg
    14881 cctgagattt gtaaagaatt aagggagaaa ttaaaacagt atcattatgt tgaaagtgaa
    14941 ttattccctg acgtatacag acatataggt tatattcaat ctaattttct atcaaatcaa
    15001 acaaataatc gtactattaa taaaaataat atatcagaaa atctagtcga tttattaaaa
    15061 ctaaaagaaa actaatacaa ttcaagacca tgcaaactca tttataaaca ttgtccgttt
    15121 atagagaggt atgcgtggtc tatttttata aaatatattt aattacaggt ttaaacatct
    15181 ataacaaaat ctcaacagca aaaaataaat ctccattaat catttatcta gactcaagga
    15241 aatcatatat ttctatgcct tttaacatat cgtcaatttt aatgagtaat ttctctacta
    15301 atattccagg caaataggca cgtgctttgg tggcttgcat ccatgctggt tttggattat
    15361 attcgccaat gtaccaatca atgactgttg gtttgatcat ttgacataat ccagccacac
    15421 caaattggcc attggattct tttgtaataa ttgcatgtaa aattaaatgc tcgattaaat
    15481 cgcagtaaac taagttttct ttcttttgat aattgtatga gtacttaaat tcggaaatga
    15541 atcttaggtc agataagttt tggaatttgt cctcgcttat gtgatgacaa tataatcctt
    15601 cgctagctct agtgtatttt ccttttgaaa tacttttgat ttctccattt aaaaatcgtg
    15661 tgtatgattt ttccttataa tagtcatcgg ttacagcgcc atatttattt aaaaggtatg
    15721 ctacagcttg cgagtaagta gcgtctaata attgactata ttcattatat ttagctgtat
    15781 attcgttttt ctccatttca ataaacctct tttcaaactt tataaaatga aatacttgta
    15841 atttagaaag aataaaatca atatccacat agttaggttc tacttttata tgtctatttc
    15901 tcttgcataa tgcgccaatt gattaatatt ttgtcattga attctacatt tttgtattgt
    15961 tttaatagtt cttcagttct ttgtaaacag actgtaactg tatcatcaat tgataaatta
    16021 aatgctgaaa agtcttctaa ctctgtccct ttgtgaatta aatcgataat gtctggtttc
    16081 acacctaggg tagagagtgt ttccatcata taaggtgtaa agagcgtatg ctctgcattg
    16141 tcgattgaaa taataccgag tttaagttga ttaaaaagac catgcatagt cattaaggtg
    16201 tatttttcaa gttgttgtat attttcaaat gttatcatag cggttctcct aaaaattacg
    16261 attaatcaaa gtataccctt atttaacttt agtttctgct gaattatttt cacggttatc
    16321 gtgtttttct tctttattaa cagcattttt atcgttttta tcatcgactt tttgatctgg
    16381 taagtcattt tgaatggctt tgttattgtc aatatcatga tgttgaatgt cttccgactc
    16441 attatccatt tcagcttgat cattcatacc tgggttgtat ttagggcttc tctttttaaa
    16501 tgcttcaaat tcttctttat ctttttctga catatcatca aaaatattaa taatcgtacc
    16561 attgtgtcga ttaacaatag cgcaacgttt taatggtgtg ccaactgcat cgcggactag
    16621 atgttcaaca taatattcat gttcattact tcgttcaaga tttgttttat aatttaatgt
    16681 ttgattttcg ttagcacctc ttgctttgta atcgtttcga acgatatctt cggcttcttg
    16741 tggagtcatt tgcttttctg tatttgactg tgtagtgtta tcagtttgtt gcgtcttttt
    16801 attagtgtca tttgatttac tactattttc tttatgttga ttagcaccac aggcggttaa
    16861 tagtgacaat gataatgtga ttgcaacgat agctttgaat ttcatttttt tatctccttg
    16921 atttgattat gtataattaa tttttccgta aaagaagggt agccgagcta ccctgaaatt
    16981 ttattctgtt gaagcgtcgt acatgctatc ttcattgtga atattttggc taggaggtgc
    17041 ttcactagtg gtaggtttgc tatggggcat gggctctaca ggagcagttt cctcattaga
    17101 ttcacttttt tctacactag aattatcact tttagacgca ttctgcttat cctcatcttt
    17161 actagacttt tcgctatctt cagaatcagt atccgcacta gaagttgtag caggtgtagt
    17221 agccgaagct tcataactta cattatcgct atttgaactc gcaacagcta cttctgtatc
    17281 ttgcttgtct tttttagctt ttgatgtttt tttagcatcg gataaatcaa atgtaatctt
    17341 cttgacgcta aataaactag aaataacttc taatttttta tcttttgcgt tggctatgtt
    17401 ttctgacaca tcgaaaacaa ttttaccttg agcagtgcta tctggattta tacgctgtaa
    17461 gaaaaatgaa ttttctatac taccattgtc actttgatta gcagacattg aacctgtatt
    17521 atctgcttca aatgttttat caccggattt tagcttaaac attgaactat caattgttaa
    17581 cgcttcttta cctttgtttt taatcgtcac atcagcaacg acaaatatac ctttagcgtt
    17641 tgttggtgca agagatggtc ctacagattt catagtttcc actgaattta cagtgacttc
    17701 aaggtcgcca tttttaacag tttcaccgat tttatgggtt ttgttcgttt ctttagaact
    17761 atttccgcca aggttacctg taataccagc tgtacaagct gaaataccga tgataattaa
    17821 aatgaataat actagacaac caccacagcc ccagaaccat ccttttttag atttcttttg
    17881 tccgtattgc tggttctgtt ggttttgttg attttggaaa gcttggaatt gttcccattg
    17941 tcttcgctct tcttcgcttt tgaattgatt gctcatgcct aaaaaatctc cctcattatt
    18001 tactatgtga ttagtgcata aagctaaata caatttgaat tcaattcgct tgttgtttaa
    18061 tatttatcag gttcacatgc acgtttatct ttatctctat ctaaatgtgg tcgatatgct
    18121 ggatgatcgg cagtgacacc attcggatat acttgtctaa gttgcttaca attagcaaat
    18181 gattgttgct tgtttgtttc agttgatggt gaagtcttag gttgcgtgga ttgatgatgg
    18241 tcacttggtt gttgcttttc ttttaaatca tctttcaagt catcttgtga ttcttcttct
    18301 gcaacgtttg gtgtgttaat ttcatcatct gattgatctg ttgttgttat ttcatcaatt
    18361 tcatgttgat tattttcagt attttcattt gcgggtactt cttcggttga ttgtaaatcc
    18421 ttatctgttt ctgattctga tttttcttca ggcatatttt cacttttatc ttcgtctttt
    18481 tgagttaatt gcgtttttga tgattgatta ctagtggaat tgtcattatt actaaacatc
    18541 attgaacagg ctgataataa gataattaat ataataaaag aaataaggca accgccgcat
    18601 ccttttaaca tgccacgtct ttcagaattt ttagcagatt ggttttgatt cttgttgttc
    18661 tctgtattca aaggtaaatc tcctctatat ttatatgtta ttttcatgat ttactacata
    18721 aaacctggat gcatttaaga gggagttatt aagtgtaaaa cgctttcact atatcacata
    18781 atcatatttt accaaattag tagtggaaat actttatttt tttcaaaact ttaaaaatat
    18841 ttttggattg taggaatgct aatttaataa ttgtaaagtt gtattactgc ttaaaatgac
    18901 acgaacatga caatatcatt taataaaata ataaatttaa tttgatataa tatagtgtaa
    18961 ttatttttag aggtgttgct tttgaaaaag tttttaaaat tcaaggggat gaaaatatgt
    19021 ttaatgctat atctactgat ttttttaagt agttgtggca caaacaataa aatagtaggc
    19081 caacgatttg aaatacaaca acataatgaa ataataggta gaatttactt ttcagcagac
    19141 tatgcacata taagaggtat agaaaaaggc actgtaaaat atttcgtaga taaagtaggc
    19201 agcaaacgtt acttatttat tgaatatatt ccggataatg tattgaattg caagccggac
    19261 ttttggaaaa cgttaaaata taaaaaggac aaggtaacat attatgtata tctcattgaa
    19321 aatttagatg atgaactatt tcatttaagt gcattacaag atatgaatca tagaccgatt
    19381 gacattgcag atgatgtggc aacaatggat aaatcaccac atcagaatga cagaataaca
    19441 ctaaaattaa ataaaaataa ctgataatag tagacgaatg atataacgat atgtattgag
    19501 cattataaca atcatatcaa tgcatcaatt cgtctacttt tttatatttt taaacataac
    19561 cactgctaga atgtcataaa atcgttataa ttataatgta aatgattttg gaggaatagc
    19621 gcatggactt ttggttatat aaacaagcac aacaaaatgg acatcatatt gcgataacag
    19681 acggtcaaga atcttatact tatcaaaatt tatactgtga agcgagtcta ttggctaaaa
    19741 ggctcaaggc ttatcaacaa tctcgtgtcg ggctatacat agataattcg attcaatcaa
    19801 tcattttaat acatgcatgt tggttggcaa atattgaaat tgcgatgatt aatacaaggt
    19861 tgacacctaa tgagatgacg aatcagatga ggtcaatcga tgtacaattg attttttgca
    19921 ccttgccact ggaattgcga gggtttcaaa ttgtatcgct ggatgatatt gaattcgctg
    19981 gaacggatat tacaacgaac ggtttgttgg acaatacaat gggtatccaa tatgatacat
    20041 cgaatgaaac tgtggtgccg aaagattcgc cgtccaacat attaaataca tcatttaatt
    20101 tagatgacat tgcatcgatt atgtttacat cagggacaac tggtcctcaa aaagcggtgc
    20161 cgcaaacgtt tcgtaatcat tatgccagtg caatcggatg taaagagagc ttgggatttg
    20221 atcgtgatac taattggcta tctgtcttgc cgatttatca tatttcgggt ctcagtgtac
    20281 ttttaagagc tgttattgaa ggatttactg tgcgcattgt tgataaattc aatgccgaac
    20341 aaattttaac gatgataaaa aatgaacgca tcacacacat ttcgcttgtg ccacaaactt
    20401 taaattggct tatgcaacaa ggtttacatg aaccttatga tttacaaaaa atattactcg
    20461 gcggtgctaa actatctgcc agtatgatag agacggcatt acaatataac ctgccaattt
    20521 ata
//
LOCUS       DAMZPP010000035        20419 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMZPP010000035 DAMZPP010000000
VERSION     DAMZPP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20419
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.035"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..3728)
                     /locus_tag="POH73_000987"
     CDS             complement(<1..3728)
                     /locus_tag="POH73_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDI6886956.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNIEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKFKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHLNRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYLPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKSVLQNMSQLQCAKSSLSLETIFHCYHQMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITKENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNYVAVQFDGVFITYQTLNARANDL
                     AQRLRNQYGVEPNDRVAVIAEKSIEMIVAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     ATPKVVITYQVVYENGKRDINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEKTTILLSGTIAFDAATFEIYGALLNGGKLIVAKT
                     EQLLNPIALEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQRPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPEAIVKDDYVLSSAQKRMYLLWKSNHKDTVYNIPFLWRLSSE
                     LNVAQLRQAVQHLIARHEILRTQYIVVDDEVRQRIVADVVADFKEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARK"
     gene            complement(4175..5425)
                     /locus_tag="POH73_000988"
     CDS             complement(4175..5425)
                     /locus_tag="POH73_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6886957.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGIFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKKHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKIVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGAFLTTLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(5811..6836)
                     /locus_tag="POH73_000989"
     CDS             complement(5811..6836)
                     /locus_tag="POH73_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HDI6886958.1"
                     /translation="MKIVALFPEAVEGQDNQLLNTKKALGLKTFLEERGQEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(7010..7369)
                     /locus_tag="POH73_000990"
     CDS             complement(7010..7369)
                     /locus_tag="POH73_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDI6886959.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESVVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(7583..8614)
                     /locus_tag="POH73_000991"
     CDS             complement(7583..8614)
                     /locus_tag="POH73_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDI6886960.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEATLR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVAVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNAQNGINQYLEYDVEGFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(8627..9388)
                     /locus_tag="POH73_000992"
     CDS             complement(8627..9388)
                     /locus_tag="POH73_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6886961.1"
                     /translation="MTRLTINKIILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWTFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(9385..10359)
                     /locus_tag="POH73_000993"
     CDS             complement(9385..10359)
                     /locus_tag="POH73_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6886962.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRASKEPSNNAQHQQVIKIGYLPI
                     THSANLMMTKKLLSQHNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEALREQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKAVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(10373..11113)
                     /locus_tag="POH73_000994"
     CDS             complement(10373..11113)
                     /locus_tag="POH73_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6886963.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGFQQKISDE
                     EINAQLKLVDLEGRGKHFPEQLSGGMKQRVALCRAHVHKPNVILMDEPLGALDAFTRY
                     KLQDQLVQLKHKTESTIILVAHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            complement(11455..11967)
                     /locus_tag="POH73_000995"
     CDS             complement(11455..11967)
                     /locus_tag="POH73_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HDI6886964.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKASSLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEAGISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            12147..12350
                     /locus_tag="POH73_000996"
     CDS             12147..12350
                     /locus_tag="POH73_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886965.1"
                     /translation="MDKESVVASLARNKKIAVETMTGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDTIKDIDENHLDDAT"
     gene            complement(12412..13371)
                     /locus_tag="POH73_000997"
     CDS             complement(12412..13371)
                     /locus_tag="POH73_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDI6886966.1"
                     /translation="MNGKKANTINRYKYFHHVDHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDSIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIIADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(14018..15505)
                     /locus_tag="POH73_000998"
     CDS             complement(14018..15505)
                     /locus_tag="POH73_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDI6886967.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGGTLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPNGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGDPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            15932..16315
                     /locus_tag="POH73_000999"
     CDS             15932..16315
                     /locus_tag="POH73_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDI6886968.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRIGLII
                     MVIIQTAVLFTFVKTLPKSNNKIEE"
     gene            16322..16648
                     /gene="isdI"
                     /locus_tag="POH73_001000"
     CDS             16322..16648
                     /gene="isdI"
                     /locus_tag="POH73_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HDI6886969.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TKDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(16713..17858)
                     /gene="cap8P"
                     /locus_tag="POH73_001001"
     CDS             complement(16713..17858)
                     /gene="cap8P"
                     /locus_tag="POH73_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HDI6886970.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGITANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKESIFITGNTVIDALSTTVQNEFVSTIINKHKGKKVILLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDALEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRSDKPDEFVPLRRK"
     gene            complement(17935..19197)
                     /gene="cap8O"
                     /locus_tag="POH73_001002"
     CDS             complement(17935..19197)
                     /gene="cap8O"
                     /locus_tag="POH73_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HDI6886971.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILPFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTKACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPSYVVDTTKQIIKALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            complement(19251..20138)
                     /gene="capN"
                     /locus_tag="POH73_001003"
     CDS             complement(19251..20138)
                     /gene="capN"
                     /locus_tag="POH73_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HDI6886972.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KGYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGQVGKSDQIDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTLLINMPSMLNKYFNKLSVFRKLFGNLTYSNTLYENNNALEVIPG
                     KMSLVIADIMDETTTKDKA"
     gene            complement(20138..>20419)
                     /gene="cap8M"
                     /locus_tag="POH73_001004"
     CDS             complement(20138..>20419)
                     /gene="cap8M"
                     /locus_tag="POH73_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HDI6886973.1"
                     /translation="QLLNVLKGEMSIVGPRPALYNQYELIEKRTKANVHTIRPGVTGL
                     AQVMGRDDITDDQKVAYDHYYLTHQSMMLDMYIIYKTIKNIVTSEGVHH"
BASE COUNT         6594 a         3884 c         2965 g         6976 t
ORIGIN      
        1 tcttttcgag catatctgct taacaacgtc atgaccacac tcataaagaa cataaaatca
       61 gtaatttgat gcttttctac atacttttga agtagctgtc tcatttgttg attcattgta
      121 aatgacatca ttgctccatt tgtcgtttta atatttggtc taacatagtc tgtcggtaag
      181 cttaaaatag gtacttcatc tttgaattga gataaccaat attgtctatg tttcgtcata
      241 tcacgatgcg acatccattc actatagtct ttatattgca atttaagtgg taacaataat
      301 ttatgttgat ataatgcgtt aagatcattc attaattgta tattactcat accatcatta
      361 ataatatgat gtgtatctat aaacaggtat gcatgtaatg gacttcgaat gtatctcact
      421 ctaatttggc ttggcttttc caaattaaaa ggtgccacaa attggcgcat gatttcttgt
      481 tcatccgtaa aatgcgtgtt aacttcttta aagtcagcta caacatctgc cacaatacgt
      541 tgtcgaacct catcatctac aacaatatat tgtgttcgta gaatctcatg tcgcgctatc
      601 aaatgctgaa ctgcttgtcg caattgagct acattaagtt ctgatgataa ccgccataaa
      661 aaaggtatgt tatacaccgt atctttatgg tttgatttcc ataataaata cattcgcttt
      721 tgtgcagagc tcaacacata atcatcttta actatagctt caggaatcac ttcatagttt
      781 tgttcttgaa ccttagcaat cgcttgcgct agttcaaata cagttggctt ttgtaataaa
      841 tcaccaattt gtaatcgttt cccagtagat gcctctatcc gattcaccac taacgttgct
      901 tttaaggaat ggccacctaa ttcaaagaaa ttatcatgaa tacctacttg attcacatgt
      961 aaaatatctg caaaaatttg gcatagcaag tgttcggtat ctgtactcgg tgctacatag
     1021 gcatccgtat cgacatagtc catgataggc aatgccttct tatctaattt cccattaata
     1081 gtaataggaa tttgctcaat atgcataaaa ttaactggta tcatgtactc aggtaaggtc
     1141 atacgtaatt gtgatttaat cttattatgt gataatgtat gcatcgcttc ataataagca
     1201 acgatatact ggtcttgatc atgattttga acaataacaa cagctttatt aataccttgt
     1261 atacgctcga gcgcatgctc aacctctgac aactcaatcc taaatcctcg aatcttaact
     1321 tgtttgtcct ttcgatataa ataatctatg ttgccatcgg gtaacaaacg tacgatatca
     1381 ccacttctat acatcagctg atttatattt gaatctttga taaatttatc tgctgtcaat
     1441 tctggctgat ttaaataacc tgcagccaac ccaaagccac ttgtacataa ttctccagga
     1501 ataccaacgc cacaccgacg ctcgccttgc atgatataaa catgagtacc aagaatcggt
     1561 ttaccaatag gaatacgatt tggaactttg ttaggtatat tatacgtcgt tgtaaatgtt
     1621 gtattttcag ttggtccata accattaata atttgaggat gcttcggtct ttgattaagc
     1681 aaatccaccc acttagcatt caatacttct ccaccaatta ataaatactt taacggtacc
     1741 aatacttcta ttcgttcact agcaatctga ttaaataatg aggaggttaa ccacatagta
     1801 ttgacgtcat tttcattgat taattgttct aacgctattg gatttaataa ttgttctgtt
     1861 ttagcaacaa tcagttttcc accatttagc aatgcaccat atatttcaaa tgttgcagcg
     1921 tcaaaggcta tagttcctga taacaaaatc gtcgtctttt catttaatgg tacataatga
     1981 ttttgatgga ccaagcgaac aatacctcgg tgcggaatta gtgtcccttt agggttacca
     2041 gttgtccccg acgtgtaaat aacatatgca tgatcttcta acgtgttaca tttagaaaga
     2101 ttatcaatat ttttccacgc tatcttgttc aaatcaatgt gattaatatc tcgtttacca
     2161 ttttcatata ctacttgata tgtgataaca actttaggcg ttgcatcttt taaaatgtac
     2221 tcctgacgat cacttggata gttcggatca attggcacgt aagccccacc agctttcaac
     2281 acacctatca tcgctactat catctcaata cttttttcag ctatgacagc gacacgatca
     2341 ttaggttcaa caccatactg gtttctcaaa cgttgtgcta aatcattcgc gcgtgcattc
     2401 aatgtttgat atgttataaa cactccgtca aattgcacag cgacataatt cggtgttgct
     2461 tcaacttgtt gctcaaataa ggtaacaact gtttgcgcat catctatctc aggtaaactt
     2521 aaattgatat cgtcatataa ttgaatatca cgttctgtca tcaaattaag ttcatctaca
     2581 gttgttcgtt tatttccatt ttctttagta atttgcaaat aaatattacg aactaagtca
     2641 ctcagcgtct cgattgagag caaatcatat gctgcactgt tatatataat tttgaacccg
     2701 tgttgatgtg gaaaaatttc aatatccgct aaagatgtat gtgcatcatg tatttgatgt
     2761 acatcctcaa taacatcatt acaacaagac atcatttgat gataacaatg aaaaatagtc
     2821 tctaatgaaa gcgaagactt cgcgcactgt aattgcgaca tattttgcaa cacactttta
     2881 ttaaaatctg ttgtaaagcg ttgacataca tcttttgcat cgattgttaa cgttaatggc
     2941 acaatatttc cgtgtaaatc atttggtaaa tgtgatggta catgtatacc taatgtgaca
     3001 tcatgttgtt gactcattat atgattagct aaatacacac taacagccaa cgatgccata
     3061 tctattgatg tcatatcatc aatcaaatac gtttgatata aagcttgctc aaatggatgc
     3121 ttaattggta aataactatc gatatggatg tcagagttat tctctagccg aaaatagttt
     3181 gagtctaatg ctatatgcga tgcatcttga ttgtctttat catcatttct atttaaatgt
     3241 gcatgctgtc gagtattgtt aataacagta ttgccacgat atgcattgca taaatcatca
     3301 agaaaaatat caatttgact atcatcgaaa atggacacat gaaaatctaa taatatatat
     3361 gcagcatcag cgaactgaaa caatttaaat ttgaataaag gtgaatcatt aaaatggtaa
     3421 gtacttaatt cttgcttaaa aaaagcttct aaatcatagt ttgcggaaga agatggaact
     3481 tgttttatct caataaaagg cagaaattca tgaagtatca ttgttaaatt gtcatcggta
     3541 gtaacatcaa aaaaatgtct tatagatgca tgttgttgca caattgtcga taatgcatac
     3601 atcattttag tagcttcaat atttttagcg agtttaaccc aatacgcatt acggtgtgtc
     3661 gttgattctg tattattttt gtatatacga aaatattcct gttgaaatct caaattgccc
     3721 ataatcataa aaagtccttc tttcatatca taatactcat tacttactga aattgcatga
     3781 tgatatgata accgacgaaa tgttaattaa ctcgttatgt aatgattaat ataaaacacc
     3841 attcgcaaca tatgagcgat atattctacc ctaaaataca tcttgtatca tcgttacaat
     3901 tggtatattt ttcaatgtaa attacataca tattcgataa ctagcacact acaactcgtt
     3961 aatcactttc tcttattcat atctcattgc aagttcaata ttgttgttac aaaatatcca
     4021 tgaagcaagt ttatattaaa caaacaactc gcataaaaca attgttatcc ttaaatttta
     4081 acaaattctt aataaattta tctctatttt aattacgacc aaattaatag gttttcaata
     4141 taaaaagatg catagaataa atatttaaat aaattcaatt tgtattcact tgtttttgtc
     4201 ccccgaatac accagcaaca agcatgctag caccaattgt taaaactata aacatataca
     4261 gtcccatttg taatgtcgtt aagaaagcac ccaacacaat ccctaaccta gctagtgttt
     4321 ctgaaaaatg aatgcctaat gcattaactg cactatatgt tcctctttta gctttaggaa
     4381 taattttaaa gcgttgttct gaaactatag gcgaataaat aatttcacct acagtcgcaa
     4441 ttatcataaa aacaactaat aagccaaact gattaagata ggttagacca ctatagccaa
     4501 caatataaat cagcaaacca gtgattaaag cttttttaaa atctattttc aatacaattt
     4561 tcgagattga atacgtgagt aaaatgacga cgaccgtatt aatcattagc aagattgcta
     4621 acatcttagc acctgtaata tcatatgaac ctatacttat tgtttcaaac tgatccttta
     4681 gtctaatagc aatatatgag gagattgaaa attcacccat catgatgata ctgaaccccg
     4741 aaatcaataa catataatta cggtctttca aaactaattt ataactgcga aatatattca
     4801 ttatttgtaa tttttgataa cgacttgcat gtttcttgtc atcactttgc tttacttgat
     4861 ttcggtcttg aggtaaccaa atatataaaa taaacagtac aattaaaaat atacaagctg
     4921 ctattaagaa aagcagtaac atactgtagc catacatcaa gccacctaac aatgctccaa
     4981 tagctaccga taagttcgtc atccaatagc taatcttgta aatataatgt tccacatctt
     5041 cggtaattgc atccataatt aacgtgtcca taactggaaa ttgcaatccc caaacaattg
     5101 taaatatggc gtatgcaaca caaaaaccaa taatttgcca caattgatgt gacccaaata
     5161 tgcccatgaa cacaagcatt atcaccatcg tcgcttgata aataagtact agcaactttt
     5221 tcggaaatat ctcaataagg taaccagata taatagacaa tggaaatttt agaaccacta
     5281 aaccaacaag gtatataccg acaattgatt gacttaacat atctgttaaa tatagtgcta
     5341 taaacggtat aaatgctgtc gtaataatta gctgtaaaaa attgctaatc aatcgtactt
     5401 tcaaagtcgt acttaaacgt ttcattgata actacacccc tatccacata tatgcacact
     5461 accatttata taaaatctat tatttgatta tacgttgaag caaagaattt ttagttatac
     5521 aaagttattt aacaaacaaa tgtatcgact ttacaatgca tcttgcatat aatgcctcaa
     5581 aaaaattagc actatatgac tatatattat tccaaatgag tgaaatttat caacatttgc
     5641 gtaagtgaac acatatgtgc gtaaacgtta aatcattgtc ctaacatagc cgtgactata
     5701 cttagatgta atcataaaaa aactactgcc atctttacaa cagcagtagt acttatatga
     5761 ttcatttata taaaattgaa agcgttaatc gctagcccag catccttatt ctatttagct
     5821 gtataacttt tactagcaat acgaccactt gcaacaataa tatctttatc ttggaaaggt
     5881 tcatgattga agaaacgctc taaaatatct ttaactccat cttcaatacg tttttgtgct
     5941 tctaaagtca tacctgaata gtgaactgtc atagcatttc taggcattgt tctccatgga
     6001 tgatcagcag gtgcaggttg tggataccaa acatcaccag catatccttg taaatgctca
     6061 gatgctaacg cttcaactaa cgcatcgcga tttacaattt taccacgtgc agtattcact
     6121 aaataactgt gttttttcat acgacttaaa acatctttat caaataagtt atcagtttct
     6181 ggtgttaatg gtgcatgaat tgtaatcgca tcacttgttg aaacaagttc atcaaagctt
     6241 acaaatttag acaatttatg gtcttgttga ttgattggat catagtgttg taatgttaca
     6301 ttaaatggcg ctaatctttc agcaacaagt tgtccaattc gtccaaatcc aaaaatacca
     6361 attgttttgt gttgtaattc atgcgcatga ttacctactt gagacaagtt ccattcacct
     6421 tctactgatt gacgatgacc ttcttcatag tttctaagaa gtattaataa atccataact
     6481 gcatgttctg ccacactaac tgtattactt cctgtaactt caacgacacc aatgttatgt
     6541 tcacttgctg ccgctaaatc tacatggtca gatcctacac ctgctgtaat tgctaatttc
     6601 aagttcggtg ctttttcaat acgttcacga gtcatatatg caggataaaa tggcgcacta
     6661 ataatcacat ccatatctgg taaatgttta tctaagtctt caccattatc tgctaatata
     6721 atgaactctt gtcctctttc ctctaaaaat gtttttaatc ctaatgcttt tttagtatta
     6781 agtaattgat tgtcttgacc ttctactgct tctggaaata acgctacgat tttcatatcc
     6841 atctctccta aatgataaat tttgacttcc ttaaagtacg tgttgcatgt tgcttgtttt
     6901 aaaaatatga caaaaaaaac ggcactgttt gacataccac ccccttgaat tattgttaaa
     6961 ttcaaagcga agttagcaaa cagcaccgcg taccttacac cagcaacact tattctattg
     7021 acttatcgaa aacaaatatt gaaatgactt catcatcatt caaatcaata tctgtaaata
     7081 attttacaaa cttagcgcct acaacacttt ccatctcatt tacatctatt tctttataca
     7141 tctgtttaat cttctctgtg cgtgtataat ggagcatcga ttcattgcgt ttgtcgttta
     7201 ggtaaaaact ctcaacttta ctcaaaacac ctgtcatatg tgcaatcgcc caattatctt
     7261 taaacgacac tcgaatactg ttgggtcctt tacccacgta ttctttacga taagatctta
     7321 ctagattcga gaattgttgt tctttagtat gcgtatctat agtattcacc ttctctaaaa
     7381 taagaagttc attaattgtt cacattttca ttatacacct tattaacact ttttcaatga
     7441 taacgattat tattacgaaa tgtatatttt cctagttaaa aggatataac aaattaacag
     7501 aaaatgttta taaactaaga tttatattat tttaaaaatt gtttcaacaa gcgcatctaa
     7561 acaacaaatc agcatatcat acttacccct tcaactctgc ctcaagttta cctaaatgtc
     7621 ttaatgttgg cgttaatgcc gcaaagaaga atccttcttt taatttacgt acttgtggcg
     7681 aatatggtgt gtacgctctt gaaccaccat tgacaacaga agcttgattt acatctaaca
     7741 ataagtagcc aatgtccttc ttcaatgata ttaattcatt taaatgtgaa gttaagttac
     7801 cgtcatctaa tattgcataa tattcctctc taagttgacg atagcgtttt ttaaaacctt
     7861 caacatcata ctctaaatat tgatttattc cgttttgcgc atttgaaaat gcatcaatta
     7921 actctaaaga acttttaatt gagcctaatc ctattggaat ttggtaagca ataaattgcg
     7981 ggcgaatagt tgccgcaaac tgcttcgcat catgcgtgat aatttgtgat tgtggcactg
     8041 cgacttgatt caaagtaatt tgatacgttg ctgagccgtt tacccctaaa aaatttgttt
     8101 tttcaacaag cgtgatacca tcttgattgg cacgtagaat gaacatgaca aattcatctg
     8161 atgattcatg cttcgaaatc gcaccaaaat aatggtcttc ttgaatatta cttacagctg
     8221 gcatacgtcc actgacaacc aattgtccat caacataagt gtgttcaagg ttcaactttt
     8281 ctaaatcatt aaatgacttc atcggattag acaatccggt agcacctaat atttctccag
     8341 ataacaattg ctgttgtaag tcattattta aatgtggctg tgtggcattt tctaaatacg
     8401 ttgaaaaagc caattggcac cataaacaaa atcctgttgt caaacaagac tgcgatacag
     8461 cttcgattac ttcagcattt tttctcagtg tcgcttcacc aaaatagcca tctacaaata
     8521 attgttggat aaatgttctc ggataatacg tcccttcatc aacttctact aaatgaggat
     8581 ctaattgttc tttgataagt gtttctaaag tcatcatcat ctctccttat tcaccaaatc
     8641 ttctaagtat aaactgctca atataactaa tgaatcgatc aataataaaa ccaaataatc
     8701 cgataaagaa tattgctgct aaaacatctt ctaagttcaa catatttcgt gcatcaacga
     8761 ttaaaaaacc taatcccgat tgtgcaccaa tcatttcacc agaaactaaa aatatccaac
     8821 ttgttcctac cgccatatgt atcccagcca tgatttgttt aaatgccccg ggaaataata
     8881 tattgcgata caatgaccac ccagttaaat ttaaatttgc cgctattttt aaatattgag
     8941 gttcaatgtc tctaacgcct ttaatggtat tgaacacaat tggaaaaaaa gcagcgataa
     9001 aaataatcgc aatcgctggc aaactaccaa taccaaacca tagaacaaca aatggtgccc
     9061 aagctatcgg agatatcggc ctaatcaatt gaaatagcgg ttcgatagcg ttgtatagcc
     9121 aacgattcct tccaagcaag aagcccaatg gaatagcaac caacaatgcg acaacaaagc
     9181 ccgctacaaa tctccataaa ctaattgcta aatgttggaa aatttctcca gtaacaatga
     9241 aagtccatat gctttttcct acaagagcag gaccaggtaa caatacaggt tggtaatgcc
     9301 caataataat gaccatttcc caaatgccta agaaaataat aaatgtgata ataggtaata
     9361 taattttatt aattgtgaga cgtgtcatga acgtgatgcc tccttataca atgacggttc
     9421 aacaaagtca tcatatgcag gtggattaaa caaatgatgt tgttttacca atgtcgtaat
     9481 ttcttgatat ccggatggct taattgttaa atcaccatag gatgtccatg ccgctgactg
     9541 tgttaaaacg tcacgacttt gtttaaaatg atgcgtcata atgtctacac tttgcttgcg
     9601 atcattcatt ttaaagccag actttttata atcttgtaca aatgcttgcg ctacagcctt
     9661 gtgttgatca agcaattcgc ctctcagtac tagcacacag caatacgcat caggtataac
     9721 gtcatcacca tgtttcaaag tcttaccttt gcctaacttt tcccccagtg caccgaatgg
     9781 ttcggctaca gaataccctg taattctgtg ttcacttaat gcggctggca tttctgctgg
     9841 cgacatttca tgatagctaa aatgccccgg tttaatcttt aattgttctc gtaatgcttc
     9901 aagtaagaga taatgtgttg aataacgatg cggtatacca aaatggtaat catcgccatt
     9961 attattaaat tcatttaagt gcataccttt ttgtcccata atgacattgc cttcatgatg
    10021 ccccaatgcc acagctttta tattcgagcc cttctgcttt gatttcatcg cgagctctat
    10081 taaagttgat gcaccatcaa tacgaccact gtttaatgcg tccattaaat ctggccaatt
    10141 attgaattta actaattcta atttatattt cggatgattg tgttgtgata ataatttttt
    10201 agtcatcatc aaattagctg aatgtgtaat cggcaaatat ccaattttaa tcacttgctg
    10261 atgttgggca ttattagacg gttctttaga cgccctttgc caatcacatc ctgtaattat
    10321 aaagattcca atgatgacga ttatgcttaa ccttttcatc gtcactcact ccttataaat
    10381 aatattcagg ttcaacttga tgatgattca atgcaaatgt ttccataatt tcattacgaa
    10441 tcttaagtag gtggctatca ttacgactgc gtggatgtga tgctgtaatt tcatattgag
    10501 aaataatatt gcacccttca cctaacagaa caatgcggtc ggaaagataa atagcttcat
    10561 caatatcgtg agcgactaag ataatagttg attccgtttt atgttttagt tgtactagtt
    10621 gatcctgaag tttatagcgt gtaaatgcat ctaatgcacc taatggctca tccatcaata
    10681 taacgttagg cttatgcaca tgcgctcgac atagtgccac acgttgtttc atacccccgg
    10741 acagttgctc gggaaaatgc tttcctctgc cttctaaatc aactaattta agctgtgcgt
    10801 taatctcttc atcactaatt ttctgttgga atccaatcct aatgttgtca ttaatcgttt
    10861 tccatggcag cagattatga tgttgaaata gcattaaaca atctggagac ggctgttgtt
    10921 taatttcgtt atcaataatg acacgaccag atgatggatg aataaatcca ccgatgatat
    10981 tgagtaaagt agacttcccg caaccacttt tccctataaa agtgactatt tctcccttgc
    11041 taatgtccaa attaaagtta tgaattactt tatgtgatcc aaagtgatgt tgtaattgtt
    11101 gtattttaat catgacaagc ccccttttaa tccctaccat tttaataaga attaaatgta
    11161 tatctaaagg gctatgccaa acacagccct taaatcgtga attgttatat ctatagatga
    11221 ttatggtttg gtatgagtac tcttataaca taagcgttat tcaattaacg acgttatatt
    11281 aatttcagct ctactaaatt tcaataaaat gattgtttta gacaaaactt gcatttagtt
    11341 cattagtttc gtcatatcaa ctacttacat ttatagcatt gagctattct aattaactgc
    11401 aatcgaaatc cacgccaaac ctatatacat atatatcatc aacttgttgt cttattaaag
    11461 tttttcgatg ccatcaatcg gtgtatcaac tgctttgcgc gcgcgacgta ccgcttcttc
    11521 atcaggtgcg ttgtataaac aaatacattt agacatatct tcacatacat atgtgcgttt
    11581 aaattcaact tctggcactt cttcataatg aacagaattt ttctttttac gtgctaaata
    11641 ttgatccatc gtaatgcctt ccggaatgtt ccaagttaca aggtaatcaa catgtgcatc
    11701 accatttttt acttcatcta aatctttacc aactaagcgt acttctttga ctaattgaat
    11761 gctaatacct gcttctgtta aaaattgttt agcttctgct tcgtcatttg cttccacaat
    11821 aaaataacca tgagttaaat tttcagtagc ttgtacttca attaatgttg gaattgtctt
    11881 cgtagatagt gatgatgcct ttgcttctag ttcttctttc gttgatgcaa aatcaagatt
    11941 gttagcttct aataaaaata atgtcattaa taagacctcc tataacaatg gttgaaaaaa
    12001 atattactat aattcttatc ggattacaag gatattgagc gattttttta aatatttaga
    12061 taaaattctg taaaatatat tttcaaagag tataaaaata atgttagggt ataagtaaat
    12121 aaagatatat atagggggct atcattatgg ataaagaatc tgtcgtagca agtttagcaa
    12181 gaaacaaaaa gattgctgta gaaacaatga ccggtcaaag gtacatcata gaacgcattt
    12241 tacatacaaa tgatgaaaaa catattcata ttttgaaacc taaagatgtc gtgttagatg
    12301 tagataccat caaagacatc gacgaaaatc atttagatga tgcaacataa gtataaaacg
    12361 aaacatgcgc ctatgacatt gattaatgtt ttaggcgcta caatgtttta tttatgttag
    12421 tttgtcgaag tatggctcat ctaatggatt caattgcaag ttttcaattt gcaacgttga
    12481 atgtgcaatg ccatattttt ctttcaacaa tgatgatact tgatcaatcg cttgataatc
    12541 atccccctca tattttttat ctaacacaac atgggcactt aatgaataat gttctgttgt
    12601 aatactccac aaatgaaatt catgtacatc taatatgcca tctatgtttt ttatatctgc
    12661 tataatttga tcagtatcca aatgttgagg cacactttcc attaaaatta accacgcatt
    12721 acgcgtaatt ttataaccac cacgtaaaat gatgagtgaa attacaatac taatgattga
    12781 gtcaatgatg cgccatcctg taaagtagat caatacaact gcaacgatga caccaataga
    12841 gttcaataag tctcccatga aatgccataa tgcactttga atattgatat tgtcttcttg
    12901 ttttaaagac cttacaagga taacagtcaa aataatattg acgagtaaac caatactagc
    12961 aatcataaac ataatgccac tttcaatggg ttgcggataa ataatacgta caatagcttc
    13021 atataaaatc cagattgaaa ttacaattaa tgctaaacca tttaaaaatg ccgctaatat
    13081 ctcaaatctt aaatatccga atgtgtagcg tgcagtcggc ttcttactag caaaataaat
    13141 tgccaacatt gacaaaccga gtgctaatac atcactaagc atatgaaatg agtctgacag
    13201 caatgctaat gaattagata ctaaacctcc aacaaattca atcactgtaa ataatagtgt
    13261 gatgataagt gatgcccata gcgtcttttt agaactttgt tgaatttttt gatgatcgac
    13321 atgatgaaaa tatttgtatc tgtttatcgt attcgccttt ttcccgttca tagtttgcca
    13381 ctcctaaata aatgcgtcgt atttaatgta gttgctttta ataacaatta tttatagctt
    13441 ctcaggttag tattaatgct tccgtaatta accataattt atacatcatc gtactttaca
    13501 tgacaactca ttaatcttta ccctttatac ctttatgtaa tatcaccttg gaaaaacttt
    13561 tacagcacac tcgcgctttg ctttatccct atcatataga aatttttaaa catttccgat
    13621 atgattgtgc taattacata aaaatggcaa aaaagtttct caaattcatt tgttttattt
    13681 catcaagtcc aaaaataaca atagtatttt atgattgttc cacatattaa aacaaacgat
    13741 acatttgcat atagaacttc atatttcaaa atcaccttaa ttcataaaca actctgtcct
    13801 tataatagcc gcatcgcaaa ttagcataag taaaaacagc agtaagatga tttgcaatta
    13861 aaaaccatca tactgctatt attttagtca ttatatccta gtatcattat gcaagttcct
    13921 aaatattttg tttcgcttaa aatctaaaca actccgtaaa catgctaaga cttaccgcta
    13981 gactgcctac aaacacgctt caaaaatgat atttattcta gtacaaacct tttaaagcat
    14041 tgcttgtatc aatataaata tttttaactt gttgatagtt acttaacgca cctttataag
    14101 tttctcgacc gatacctgat tttttataac caccaaatgg tgcgccttct ggtacttggt
    14161 tgtaagtgtt aatccaaata cgtcctgtac gaacagcttt agcaatattt aatgcacgtg
    14221 tgatattttg agaaaatacg ccgccagcta aaccatactc tgaatcatta gctatatcaa
    14281 ttgcttcttg atcatccttc actttaatca ctgttaaaac tggtccaaat atttcttctt
    14341 gcgctaattt atgatgatta tctggcacag caattaacgt tggttcaaag aagaatcctt
    14401 tatctaaccc attttcagtt aaacgatgac cacctgctaa aatttgtgca tctgattctt
    14461 ttgctgcatc aatatatgat tgaattttat ctaattgatc cttaccagtt tgactaccca
    14521 tttgtgtagc ttcatcttgt ggatctccaa ctttaatatt tgaaaatgcc tcttgtaaac
    14581 gtggcaccaa ttgatcataa attttttcat gaactaataa tcgagaacct gcactacata
    14641 cttcaccttg gttgaataaa atacctaact gaataccttc aactgcaagg tctaaattag
    14701 catcatctaa tatgatattg gcgcttttac caccaagctc taaagtagca ggtactagat
    14761 gtttcgctgc agcttcggca acttgataac ctacatcagt tgagcccgta aatgataatt
    14821 tatctacccc atcatgattg aaaattgcat tacctgattc tgaaccttta cccgttagta
    14881 tattgacaac accattaggt aatacctctt ggaaaatttt agcaacttcc aataaactta
    14941 atggtgttga agacgaaggt tgaatcacaa ttgtattacc tgcagcaatg gctggcgcaa
    15001 tcttccatgc agctaatagc attgggaagt tccaagcaac aacagcacct acgacgccaa
    15061 tcggctcatg tcgtacgata ctcattgtgt ctttatcgat atcattcact gtaccttctt
    15121 ctgtttcaat aacacttgcg aaataatgga aatgtcttgc agcaaatgga atatcaattg
    15181 ctgttgtctc acgaatcggt ttaccattat ttaatgtttc aatcattgca attttatctt
    15241 tttgtgccat taatttatca ccaatatcac gcaacatttg tgcacgttct gatttagaag
    15301 ttaatgacca tgattcaaat gcctcttgcg ccactttgac tgcatgatcg acatctttat
    15361 cttttgctct tgtaatatgt gatagtgttc ctccagttgc tggattagtc acttcgattg
    15421 tctcgtcact gctaccttta acaaattccc cattgataaa taaaccataa ttctctgcta
    15481 tataatctcg aacgtttact gccatattat ctctcctttg tttattggct ataatataaa
    15541 tctcttagca tcatatttaa taatgctttt aaacgatatg attcacatta tatagtaagc
    15601 gtttacattt tataaaataa tcagctcaca gaagtctcat ttttaaaact tttgcgtcgt
    15661 ttttgcacat aaaaataatc ttattttcga aaacatattg cacttttcaa aaataaaaca
    15721 cataatataa ctataatact tgagaataat tctcattaaa tagtttggtt gttatgaaag
    15781 tcgcgcttaa aatgcgttaa tgggacaagg ataattcaac aacgtttcat cttactgagt
    15841 cattgcactt atcatacaca ttatatttag catgagttat attactaaaa caatattaca
    15901 tgcgcattag tactagaatg gagttttttc aatgcgactt attttaatcg tcattggctt
    15961 aatatttaca gcgcttggta ttgcaggtgc cgtattacct ttactgccaa cgaccccttt
    16021 tttactcgta gcagtttttt gttttgctcg aagttcagat cgcttttaca attggcttat
    16081 taatcaaaaa atttataaag aatatgtaga aaacttttat ttacatcgag gctacacgct
    16141 acaacagaaa attaaaattt taattagctt atacattgta ataggttttt caatttatat
    16201 ggtggatgtt cttgcagtcc gtataggatt aatcataatg gttatcatac aaaccgctgt
    16261 actctttaca tttgtaaaaa cattacccaa atcaaataat aaaatagagg agtgattgcc
    16321 catgtttatg gcagaaaata gattacaatt acaaaaaggc agtgcggaag aaacgattga
    16381 acgtttttac aatagacaag gtattgaaac tattgaaggc ttccaacaaa tgtttgtcac
    16441 taaaacatta aataccaagg atacagatga agttaaaatc ttaactattt gggaatctga
    16501 agatagcttt aataattggt tgaattccga tgtatttaaa gaagctcata aaaatgtacg
    16561 tttaaaaagt gatgacgatg gacagcaaag tccaatatta tcaaataaag tattcaaata
    16621 tgatattggc taccactatc aaaaataaac atattaaaaa cccctagtca ccgctggttg
    16681 tctaaaccaa cttaatgatt aggggttttt tattatttgc gacgtaaagg tacgaattca
    16741 tccggcttgt ctgaacgcaa tccaaaataa tattctattg cttcacaaat acgtcgtgat
    16801 gcttgtccat caccatatgg attattcgct tgactcatac gttgatacgc ttcatcatcc
    16861 tcaatcaatt gtttcacatt tcgaacaata ttgtcataat ctgtgccaat tactctcgat
    16921 gttcccgcct caacgccttc gggacgctct gtatgattcc ttaataccaa cacaggtttt
    16981 ccaaatgtag gagcctcctc ttgaatacca ccagaatctg tcagcacgag gtacgattga
    17041 tttgtaaaat tatggaactc aagcgcatct aatggctcaa ttaattcaat ccgatttctc
    17101 ccagataaat atttttcggc aatcgctctt acctttggat tacgatgcat tggataaatg
    17161 aagacaacat ctttatattc atctgccaaa tctcttactg ctttaaaaat ctgatgcatc
    17221 ggttctccaa tattttcacg acgatgcgct gttagtaaaa taactttctt gcctttatgt
    17281 ttattaataa tcgttgaaac aaactcattt tgaactgttg ttgacaatgc gtcaataact
    17341 gtatttccgg taataaagat actctctttg tctttgtttt caaaaagtaa atttttagct
    17401 gcaattactg ttggcgcaaa attcaattca gcaatattac ttaccatgac tcgatttaac
    17461 tcttcaggaa atggtgagta tttctgatgt gttcgaagtc cagcttctac atgtccgacc
    17521 ggaatttgat gataaaatgc tgccaagctt cctacaaaag tcgttgtagt atcaccatgt
    17581 actaaaatca tatccggctg ttcctcatta atgatgctat caagtttagc aagcgcattc
    17641 gccgtaatgc ccgctaacgt ttgttgatct tgcataatat ttaaatcatg atcagcttga
    17701 atatcaaata tacttaacac actatctaac atatctctat gttgtgctgt aatcacaatg
    17761 ttcgcttcaa agttcccatt atgatcaatt tcttttacta atggtgccat ttttattgct
    17821 tcgggtctcg taccgaaaat aaccataatt tttttcataa cattatcctc tctgaagttc
    17881 aaacacatga cagcttatct ttccttaatc atgtatgccc tagtttgaca cattttattt
    17941 gtcgataaaa ttaaatatat tgccataatt ataatacgat acatcttcaa atgatgattt
    18001 cacaacattt tttgtatcaa aaatcacttt atgcttcatt ttatcaaaat gactgtccga
    18061 taaattttta aattctgagt ggtcacttaa aatcaatact agcgatgcgt ctttgacagc
    18121 atgtgacata tcatgttcca caaaatctaa ttcaacatgt ggatcataag cacatacttc
    18181 tatgtctggt tcttgattta atagctcata aatatcaaat gctggtgatt ctcttatatc
    18241 atcaacatca cctttataag ttaaaccaaa tactgtgact ttattcccgc tcaacgcttt
    18301 gatgatttgc ttcgttgtat caacaacata ggacggcatt gaattattaa tttcacgtcc
    18361 agtttgaatt aactttgcat tttcagggtc tttagcaata ataaagtacg gatcaacagc
    18421 taaacaatga ccgcctacac ctggaccagg ttgatggata ttaacacgcg gatgtttgtt
    18481 tgccatttca atcacatcta atacattaat atttaagttg ttgcaaattt ttgttaattc
    18541 attagctaaa gcaatattca cgtctctata tgtgttttcc attagcttac tcatttcagc
    18601 agtacgtgca tctgtttcaa tcatttctcc ctgaacgaat gtgcgataga cacgtttccc
    18661 cgcttcaata caagctttag tcacaccgcc aatgatgcga ttgttatgaa ctaattcttc
    18721 taaaattttt cctggcagta cacgttctgg acaatgcact aaataaatat cttcacctat
    18781 tgtaaaccct aaattttcaa tgactggttt tacaaaatca tccatcgttt taggcgcaat
    18841 tgtcgactct acaataatgg tatttccttt ttctaaaaat ggtaaaatac tatctaatgc
    18901 acgcataact agcgaaatgt cacatgaccg gtactgatca tcattattcg gtgtcggaac
    18961 ggcaatgata aaaacatcag atgcttctgg cgttgtagat accttcaatt ttcccgatga
    19021 cagtacctct tcataaacct cttgtaatcc aggttcttca atactaattt gaccactttg
    19081 taacttatca atcgtttgct gattaatatc aacaccaagc acatcgacgc catgctttgc
    19141 aaacataatt gatgttggta aaccaatata acctaagcca actactgtta acttcatact
    19201 ataacctcca aataaaacga tttgtatgtt gattttattt aatagatgac ttatgcctta
    19261 tctttggttg tcgtttcatc catgatgtcc gcaataacaa gtgacatttt tccaggaata
    19321 acttcaagcg cattattatt ttcatataaa gtattgctgt atgttaaatt gccgaataat
    19381 tttctaaaga ccgacaactt attaaaatac ttatttagca ttgaaggcat gttgatcaat
    19441 agcgttttac gatgcgattg gcgacgtatt tcatacatta ctgacgatgt atcaaagtaa
    19501 aaactatctt gaggatgata cacgcctgtc acttctaatg atattaattg atcaataaat
    19561 gctgtcagat gtttaatata taatgcactc cgctgattat taatatttgg aatgattggc
    19621 aatcgctttg acaattgcat taaccgttgg aaatttcctg ggcaatgtgc accataaatc
    19681 attggtggtc tcacaattgc tactttaaac gaatcactaa tcaactcttg taatgcttgt
    19741 tcagcgaact ttttggaaat accatagttg gtcgtagggt tcattggtgt ttgtgtatca
    19801 atttgatctg atttaccaac ctgaccttct tttccataaa ctgccatagt actcataaaa
    19861 ataaattgtt taacgtcttc agctttagcc ttttgtgcca attgtttcgt aagcaacata
    19921 ttcacttgca tataatcaga tagccttgct tgaggtgaat tgttgtgaac caaagctgct
    19981 gtatgaatta aaacatcata acctttgaac gaggtcgact tccataattg attcctaaca
    20041 ttaatttgat ccacttgatg tccttgttca ataagcttat cttttaaagc attaccgata
    20101 tatccatgta cgcctgtaat taaaatattt tttctcatta gtgatgcaca ccttctgaag
    20161 taacgatatt tttaattgtt ttatatatga tatacatatc aagcatcata gattgatgtg
    20221 ttaagtaata atgatcatac gctacttttt gatcatcagt aatatcatct ctccccatca
    20281 cttgagctag ccctgttaca cctggtctaa tcgtatgcac gttcgctttt gtacgttttt
    20341 caattaattc gtattgatta taaagcgctg gtctaggacc tacaattgac atttctcctt
    20401 ttaaaacatt caataattg
//
LOCUS       DAMZPP010000036        19875 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMZPP010000036 DAMZPP010000000
VERSION     DAMZPP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19875
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.036"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..133
                     /locus_tag="POH73_001005"
     CDS             <1..133
                     /locus_tag="POH73_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDI6886974.1"
                     /translation="DTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            complement(444..1919)
                     /locus_tag="POH73_001006"
     CDS             complement(444..1919)
                     /locus_tag="POH73_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6886975.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKVLVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTSSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSLFNDKIPYIKEPKLTKQNIEQAKTLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEDYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNNNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(2117..2473)
                     /locus_tag="POH73_001007"
     CDS             complement(2117..2473)
                     /locus_tag="POH73_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDI6886976.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLYGAFKEKYEKELRDNETQKEAFEKLKAIVNQHHHVLLLYSAK
                     DTKHNQAVVLQQLLNT"
     gene            complement(2630..2803)
                     /locus_tag="POH73_001008"
     CDS             complement(2630..2803)
                     /locus_tag="POH73_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886977.1"
                     /translation="MKLNQRYVKVFALYFASIVTANIVVKNNNLIKTLIQTLAGYTVF
                     AVGLKYLTKRKNK"
     gene            complement(2828..3973)
                     /locus_tag="POH73_001009"
     CDS             complement(2828..3973)
                     /locus_tag="POH73_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HDI6886978.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGENL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETDAYDFSQYVPGQYITVDVSSDKLPYRAKRHYSIVSGDKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIANHYDNAKLYTHLKGEQGYIGAEELKA
                     FLANKPEIYICGGTKFLQSMIKALKSLNYDMDRVHYETFIPRLSVVV"
     gene            4128..4235
                     /locus_tag="POH73_001010"
     CDS             4128..4235
                     /locus_tag="POH73_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886979.1"
                     /translation="MSVCTPTIARIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            4546..5499
                     /locus_tag="POH73_001011"
     CDS             4546..5499
                     /locus_tag="POH73_001011"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDI6886980.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDAEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(5820..7349)
                     /locus_tag="POH73_001012"
     CDS             complement(5820..7349)
                     /locus_tag="POH73_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI6886981.1"
                     /translation="MKYLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            7713..8648
                     /locus_tag="POH73_001013"
     CDS             7713..8648
                     /locus_tag="POH73_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDI6886982.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIVVAKGADRPLIKLVAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADKHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            8758..8861
                     /locus_tag="POH73_001014"
                     /pseudo
     CDS             8758..8861
                     /locus_tag="POH73_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8986..11082
                     /locus_tag="POH73_001015"
                     /pseudo
     CDS             8986..11082
                     /locus_tag="POH73_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
     gene            11067..11534
                     /locus_tag="POH73_001016"
     CDS             11067..11534
                     /locus_tag="POH73_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6886983.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLKYN"
     gene            11557..11835
                     /locus_tag="POH73_001017"
     CDS             11557..11835
                     /locus_tag="POH73_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI6886984.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            12062..13321
                     /locus_tag="POH73_001018"
     CDS             12062..13321
                     /locus_tag="POH73_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDI6886985.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQVICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQRVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     DSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            13339..14394
                     /locus_tag="POH73_001019"
     CDS             13339..14394
                     /locus_tag="POH73_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDI6886986.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCESKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEITFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKALLKPLDLDENEGEN"
     gene            14396..14542
                     /locus_tag="POH73_001020"
     CDS             14396..14542
                     /locus_tag="POH73_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886987.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            14566..15609
                     /locus_tag="POH73_001021"
     CDS             14566..15609
                     /locus_tag="POH73_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDI6886988.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIVKVRATGICGSDTSR
                     YKKMGPYIQGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDIAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            16137..16853
                     /locus_tag="POH73_001022"
     CDS             16137..16853
                     /locus_tag="POH73_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDI6886989.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNTQGIN
                     DDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSAQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            16846..17871
                     /locus_tag="POH73_001023"
     CDS             16846..17871
                     /locus_tag="POH73_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDI6886990.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEKHDVIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKIYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            17893..19587
                     /locus_tag="POH73_001024"
     CDS             17893..19587
                     /locus_tag="POH73_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6886991.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFLCRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKANYVLANEAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIDITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKANITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMENELPLIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDYREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     YESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
BASE COUNT         6735 a         3027 c         3762 g         6351 t
ORIGIN      
        1 tgatacgttg aaaatattta ttttaatcgt tattgtgtgg atacttgtat tcattttaat
       61 gggggcaggt ttaaaacaag tgaatttact gcagaaagaa tttaaagaac aacagaaaaa
      121 agtagacgaa taataattaa agcttatgtt gcacatatgt ttgtgacata agctattttt
      181 ataatttgtc tttaaaagag tggaagtgga atactttttg gagttaaaaa agtgtttcac
      241 gttaaacaaa tagtgacaat taggtttata taaaatgaac atgattcgct gaaagtatgt
      301 aataatcatt ttattgaaat tcatcaaaca gaaagtaata caatcatata agcaaattaa
      361 accacgccat catcatctag aatgacttcg gcgtggtttt tattgaagcg gggctgagac
      421 ataaatcaat gtcccacatt cccttatcgt tcaatcgtag ttcgataatc gattaaatag
      481 ataccttcag gtgttacttt ataattttta accttagagt tagcagcaac tatttgatcg
      541 ttgtaagcaa tataactgtt aggtacatct cgacttgata atttaataat atcattagaa
      601 atattgtgac gttccttaac atctacagta tgattcaatt gattaattaa atcatcaacg
      661 ttgttattat tgtagtctcc tttattaata gcaccatctt ttttatatgc ttgattaaag
      721 aaatagcctg tatctccacg aggaatcgtt ccgaaactat acattgttgc atcccatgca
      781 gaacggtctt ttaagtaatc ttctatgtca tcaacacttt taatgtcgat ttcaatattt
      841 gcttttttag catctgattg taatacttgc gcaattttcg atagctctgg acgaccgtca
      901 tacgtaatta acttaatttt taaagggtgt tcttttgtgt aaccatcttt agctaataac
      961 gtttttgctt gttcgatatt ttgtttggtt aacttaggtt ctttaatata tggaatttta
     1021 tcattaaata gactcgttgc aggtttcgcg taaccttgat aaatatgatc tgcaatacct
     1081 tgtctatcaa tgatatgatc taatgcttca cgaacggatt tagtcatttt tttattagta
     1141 tgattataca taagtaaaga agttctaaat ccagattctt ttgacacttt taaattttga
     1201 ttattttcta tgtcttgaac tttattaact gggacatcag ttattaaatc atctttttga
     1261 gattctaaat ttctgacgcg attattgccg tcttcttggt acgtcacagt aatatgatca
     1321 agtttcggtt taccttgcca atagtcctta aaattcgaca atgatatttt tcgagattgc
     1381 ttataatctt taatttggta agggcctgta ccaacaggag tttgattaac atctgattta
     1441 gcatctgtat cataaattgc cataaaagga ttagctaatt cagatacaag ttcagggtaa
     1501 gcggaactgg ttttaattgt tagtttttga cctttagcgg taattgatga tattggtaat
     1561 gaatatttga ccaagtcgct ttttttcatg ctattttcaa ggctagattt cactttttct
     1621 gcagtcaatt tttgaccgtt ttgaaattta atattatctt ttaattctat atctaacgtt
     1681 gtatcatttg gttgatgata cgatttcact aatacttttt ctatttttcc ttgatcattt
     1741 gttttaaata atgattctgc agcaccgatc ttaactggta catctgtttc ataaggtgca
     1801 atagactttg tttttaacgg taacgaaata tttaagtctt tgccagatga atgcattgag
     1861 ccacatcctg ataacactaa tactgctgaa aatatagttg ctagtctttt aaacttcatt
     1921 tcattaacac tctctttcta attactatgt aaaacccaac aattaatatt ttaaaacttt
     1981 attttgttga agtaaaatgt tgttcaagtt cagtaattat taaagttcaa ttaattgtag
     2041 taattacact ttttaaaaat aaatattaga aatgaagtta gcgacatgta tagtgatcca
     2101 cgataaacat atatatctaa gtattgagca actgctgtag tactacagct tggttatgtt
     2161 tagtatcttt tgctgaatat aacaatagaa catgatgatg ttgatttaca attgccttta
     2221 atttttcaaa agcttctttt tgcgtctcat tgtcacgcaa ttctttttcg tatttttctt
     2281 taaaagcacc ataaagttta ggatcatgtt ggaaccattg tcgtaactca gtagaagggg
     2341 caatgtcttt taaccaataa tctaggttag cagttctttt cgaaatacct cttggccaga
     2401 ctctatcgac taggatacga atagcgtctg tattatcttt attgtcataa atccgtccaa
     2461 tatctacggt catcttgtga actcctttct tatgaaattc agtgagcata catcaatgca
     2521 tgttgtggtg ggacgacgaa ataaattttg cgaaaatatt atttctgtcc cactcccaat
     2581 taaaaaacaa ccatgacaaa gtaaagtcat agctgttttt gtatagatgt catttatttt
     2641 tacgtttagt taaatacttc aaaccaactg caaagacggt gtaaccggca agtgtttgaa
     2701 tcaatgtttt gattaaattg ttatttttga caacaatatt tgcggtaaca atactcgcga
     2761 aatataatgc aaatacttta acgtaacgtt gattaagttt catatgagca ctaacctctt
     2821 ttctcagtta tactacaacg cttagtcttg gaataaatgt ttcgtagtgt acgcgatcca
     2881 tatcgtaatt taaagattta agtgctttga tcatagattg taagaatttt gtaccaccac
     2941 agatataaat ttcaggttta tttgctaaaa atgcttttaa ttcttcagca ccgatataac
     3001 cttgttcgcc ttttaagtgg gtatataatt tagcgttgtc ataatgattt gcaatactgt
     3061 tgaagttgtc tttgaaaggt aaatgttgtt cattttcagc aacttgaacc atctgagtat
     3121 ctaaaccttt ggcagaggca gcttcataca ttgctactaa aggtgtgacg ccaatacctg
     3181 aacctaagaa aagttgtggt tcagtcgtat tctctaatac gaatccacct acaggtgcag
     3241 ctaaattaat catatcgcct tctttaatct catcgtgtaa aattgttgaa acttcgcctt
     3301 catgttctgt tgtgacatca cgtttaacgc caaaggttaa atgattttta tcacctgata
     3361 cgatagaata gtgacgttta gctctatatg gaagtttatc actagaaaca tcaactgtga
     3421 tgtattggcc tggtacatat tgactaaagt catatgcatc agtttcaact gtaaatgatt
     3481 taatgtcttc agattcttgt ttaatattgg taattttgaa tggtttaaaa ccaatccaca
     3541 tcatttgatc ataaatttct ttttcaattt ggatgaacac atccgcaata acgccatatg
     3601 ctttcgccca agcttgaatg acagggtcat tttcttctaa tcctgtcacg tcttgaatgg
     3661 cttttaataa attttccccc acaattggat aatgttcagc ataaacttgt agtgcgcagt
     3721 gtttatatgc aactggcata ataactggtt taataacact taaattatcg atattaaccg
     3781 ctgcggccat tacagcttgt gctaatgctg aagattgcat gcctcttttt tggttcgttt
     3841 gattaaacat atttaaaagt tcaggatgtg ccttaaacat ttttggataa aagattgacg
     3901 taatttctgt tcctttttct ttaagcaatg gcaccgtttg tttgataatg tctttctctt
     3961 gttctgtaag catgatactc ctcctttaat ctgtctattt tgattattct actaaaaatt
     4021 ccgatattca attaattggt ttgagaaaat ataaataaaa tggcaaattt gataattgta
     4081 tgacattttt aattttttaa atacttacca ataagcattg tgtacaattg tctgtttgta
     4141 caccgacgat tgcgcgcatt tatttgacta attcaaaaaa cattgttgtt ttcttagaaa
     4201 aaagtaaaca tgataataaa aatgtgaaag tgtaaataat cactggcgaa gtacgaagac
     4261 taaagacatc taagatgtaa tcgtatacaa attaaaaagg tgtaaaaatt aaaataaaat
     4321 gtgaaataaa tcacaattta atattgaccc agtacttaat gcatgttaca ttttatatgt
     4381 gaaataaatc acaaacttaa aagcggatga cacatgacct tttaagttat gcgttgaaaa
     4441 taaaagagat gtttatttgc ttttgtatcg tcaataagca gcattaaact aacatatttg
     4501 aaactacacg tatgttagtg aattaatcat aagggagttt ttgtaatgaa caaatttaaa
     4561 gggaacaaag ttgtattaat aggtaatggt gcagtaggtt caagctacgc attttcatta
     4621 gtgaaccaaa gcattgttga tgaattagtc atcattgatt tagacgctga aaaagttcga
     4681 ggagatgtta tggatttaaa acatgccaca ccatattctc caacaacagt tcgtgtgaaa
     4741 gctggcgaat acagtgattg tcatgatgcg gatctagttg tcatctgtgc tggtgctgca
     4801 caaaaacctg gagaaacacg tttagattta gtatctaaaa acttgaaaat attcaaatca
     4861 attgttggtg aagtaatggc atcaaaattt gatggtattt tcttggtagc tacaaatcct
     4921 gttgatattt tagcgtatgc aacatggaaa ttctctggtt tacctaaaga acgtgttata
     4981 ggttctggta caattttaga ctctgcacgc tttagattat tgttaagcga agcgttcgat
     5041 gttgcgccac gtagcgtcga tgctcaaatt attggtgaac atggtgacac tgaattacca
     5101 gtatggtcac acgctaatat tgcgggtcaa cctttgaaga cattacttga acaacgtcct
     5161 gagggcaaag cgcaaattga acaaattttt gttcaaacac gtgatgcagc atatgacatt
     5221 attcaagcta aaggtgccac ttattatggt gttgcaatgg gattagctag aattactgaa
     5281 gcgattttca gaaatgaaga tgctgtattg actgtatcag cattattaga aggcgaatat
     5341 gatgaagaag atgtttatat tggtgttcca gcagtcatca atagaaacgg tattcgcaac
     5401 gtcgtagaaa tcccattaaa cgacgaagaa caaagcaagt tcgcacattc agctaaaaca
     5461 ttaaaagata ttatggctga agcagaagaa cttaaataac tttttataaa atctatacca
     5521 ttccaaaaat tgtaaaacct taccccaaaa aattgtataa agggctattt gatacaacta
     5581 tacgtgtcga gtagccctat ttttaatgta gtgaaagtcg ttgttgaaat taaatttaaa
     5641 tctggcactt aagctttaat catagcatta agatgattgt ctagcagagg cgatttgcgg
     5701 actcactaca gtgcatgatg aacttaatgt ttcaaatgca acattaaaaa taaaagcagc
     5761 gatgtcactt cttactccgt acatcgttgc cattagttct gttgatattt cggttagtct
     5821 taatccccga gcaattcttc aatttcattt ttgataactg taacgtgagg accgtaaatt
     5881 acttgcacac cagtgccttg ctggattaca cctttggcac cagtactttc gagtagtact
     5941 ttatcgactt tgtcattttg atgaagtgta acgcgtagtc tcgttgcaca acagtcaacg
     6001 atttcaatgt tatccttgcc tcccaaacca gcaacaatag tttgtgctct ttcagtagcc
     6061 tcaacttgtt gtgctgcagc tttatcttca cgaccaggtg ttttgaaatt aaatttcgta
     6121 attaagaatc tgaaaacgat gtaatacaaa cagaaccaca caattccaat aggtatgaca
     6181 tataggtagt ttgttttact attaccttgt agcacaccaa aaagtaagaa atcgataaag
     6241 cctccactga aggtttgacc aattgtaatg ttgaaaatgt ctgccatcat aaatgctaat
     6301 ccatcaaaga aggcatggat tacataaaga ataggtgcga caaacaagaa actaaactct
     6361 aaaggttcgg taatacctgt taaaaatgaa gtgagtgcag cggataacat taaaccgccg
     6421 acaacttttt tatgttcagg tttagccgtg tgataaattg caagtgcggc accacataag
     6481 ccgaacatca tcgtaataaa acggcctgac ataaagcgtg acacacctga ataatacttc
     6541 gtcacatctg gatcaccaag ttgagcaaag aagatgttct gcgtaccttg aactaagtga
     6601 cctttgactt ctaaagtacc accaagtgcc gtctgccaaa atggtaagta aaaaatatga
     6661 tgtaaaccga gtggacctaa caatcttaag atgaagccat aaacaaaagt accgatggca
     6721 cctgttttcg ttacaaatcc acccacatga taaatgccgg cttgtatgct tggccaaatg
     6781 aaaaacatca atacacctaa aaagattgcg gcaaatgctg tgacaatagg gacaaatcta
     6841 gagccaccaa agaaacctaa atacggtggt aataccactt tgtgatattt gttgtgaagt
     6901 attgcggtca taatacctgt gataatcccg ccaaaaacac cggtttcaac cgtttgtata
     6961 ccgagcacca tgccttgtcc attttgtgca agctgatctt ttgccaatgt gcccgtgata
     7021 gttaataagc cattcatagt tgcgttcata attaagaaac cgagcagtgc agctaaacct
     7081 gcagtacctt tatcgcttct agataatccg attgcgacac caattgcaaa gatgactggt
     7141 aaattttgga aaacaatact acctgcagct gacattaatg taaaaatatt ttgtaataag
     7201 gtaatatcta aaatagggta tgctttaacg gtgtttggat tacttaatgc accaccgata
     7261 cccaacaata gacctgcagc tggtaagatt gcgataggta gcataaagga cttgccgaac
     7321 tgctgtgctt tttcaaataa atatttcatc aacatccctc ctaattattc tcaatatagc
     7381 ttttgagaaa tttaatatca atatatattc tgtgtatgaa aatatttttc ataaaaattg
     7441 tttgaatcat gtaacaatca tataaattgc tgtttatatt gtggtgaaaa tgagttgaca
     7501 aaagtcggta tagttctgta gacatcctat tttttagcga ggtggggtga caaggcatat
     7561 cggataaatt tataagccat aaaatagata aatagtgttt gtatccaaaa ctatgtgaaa
     7621 gttaaaacta tttttcaaaa taaacatgat tcaccacata aataaatata cataagctat
     7681 cgataacaac ttaagaaagg tggatatata taatgaaaag aaagattatt atggattgtg
     7741 atccaggaca cgatgatgca atagcattaa ttttagcggg ggcaattgac agtccactag
     7801 agatattagc tgtaaccaca gtcgcaggta atcaatcagt tgacaagaac acgacaaacg
     7861 ccttgaacgt attagatatt atgggacgcc aagatatagt agtagcgaaa ggtgcggata
     7921 ggccgttaat taaactagtt gcctttgctt ctgaaataca tggggaatct ggattagatg
     7981 gtccgaaact accgtcgaca ccatcacgtc aagcagttgc aatgccagca tcagatgtga
     8041 ttattaacaa agtgatgacg agtgatacac ctgtaacaat tgtagcgaca ggtcctctta
     8101 cgaatgtagc aacggcattg attcgtgagc caagaatcgc tgagcatatt gaatctatta
     8161 ctttgatggg tggtggtaca tttggaaatt ggacgcctac agcagaattc aatatttggg
     8221 tagatgctga agcagcgaag cgtgtttttg aaagtgggat tactataaat gtgtttggtt
     8281 tagatgtaac acatcaagtt ttagccgata aacacgtgat tgaacgcttt gaaagtatca
     8341 ataatcctgt tgcacagttc gtggtagaat tattgcaatt ctttaagaag acatacaaga
     8401 ctcactttaa tatggacggt ggtccaatac atgatgcttg tacaattttg tatttgttac
     8461 aaccagaatt gtttacaatg gtacccgtta atatcgacat tgaacatcaa agtccactaa
     8521 cttatggcac tatggctgta gatttaaatc atgttacagg taagcctgcc aatgcctatt
     8581 ttgctacagc agttgatgtt gaagaagtgt ggaacttgat agatcataag ttacgtacat
     8641 acgaataata atacttaatt aaatagatac agttaaccct aaggtgcctg atttaagcgt
     8701 ccttagggtt tttgtattgg attacaatgg tcatggcaat ttgataatga ttgagatatg
     8761 tatgttaaaa atgcttcaaa aaacacaaaa tacaaaaata tatgcaaaaa tttcaattgc
     8821 tttattttaa aagattaaaa tcacaaaaaa taccgatata attggaatgt tgtcgaaaca
     8881 tcacaatgat gtacatactc attttgaaaa tatgtgttca acgagtgtac atcggtgtga
     8941 cgattgatga taaattgagc tcagatgctc aaaggaggat cgaacatgaa cggggataat
     9001 cagcaaatac tcagagaaat tgtattgaat cctactattc atggtaaaga acttgaatcg
     9061 atatttggtt tgtctcgtag acaactagga tatcgcattc aaaaaatcaa tttgtggctt
     9121 gaacaagagg gttatccaaa acttgaaaga acaagccaag gaaattttat tgtaagttct
     9181 gaaatcatga cgttattcaa acgagatgta tcggagcagc aaatgttaaa cggcaacaat
     9241 gtcattttta gcatagaaac acgtcgttat tatttaatgc tcatgctttt tagtaaggaa
     9301 aacgcaatgt ctctaaacca tttttcaatt gatttacaag tcagtaaaaa tactgtcatt
     9361 cacgatataa atcatgtgaa agagcaattg gaagatcatg gtttgtcact taagtattct
     9421 cgaaaacatg gttatgaaat tgttggtgat gaatttgaag ttcgccgttt cttcattaag
     9481 ttgattgatc aaaggttaaa tcatgatatt actaaaagtg aagttttaaa ggcgctcaac
     9541 ttaacattcg aagatatcgc atatcaaaaa gacaagatca aacaggtaga acaatttttg
     9601 aagagtcgct ttatagacaa atcactgagt tcattgcctt acgtcctttg tgtgattcgt
     9661 agacgaattc aaagtggtca tgtgattaat ccattaaata ttaattatca gtatttgagg
     9721 gatacgaaag aatatcaagc aacggagatt atgacgcaac atgagccgga tttgccagaa
     9781 gcggaaaagt tatatttgac gctacactta ctttcaacaa gtgtgcaatg gactgatttg
     9841 caggaatcag ataacatatc gaatttaacg atggccattg atcaaatgat tcaccatttt
     9901 gaacaaatta cttttattaa cattgaagat aaggagaaat tatcacagca actcttgtta
     9961 catttaacgc ctgcttttta taggattaaa tataacttaa cggatcgtga tgaattaata
    10021 aatcctttac aaggaaatta tcaatcctta tttcatatgg tgaaacaatc atgtcaatcg
    10081 ttaactgaat atttcggaaa atcattgcct gataatgaaa tagcatattt aaccatgttg
    10141 tttggaggta gtttgagacg tcaagatgaa aacttcgatg gcaagataaa agctattatc
    10201 gtgtgtacac aaggtacgtc agtatcacaa atgatgttat acgagttgcg aaacttattt
    10261 ccagaaatta ttttcttaga tgcgatttca cttagaacat ttgaaaatta cacattagat
    10321 tatgacatcg tcttttcacc aatgtttgtc ttaacacata aaaaattatt tatcacaaaa
    10381 gtagctttat ctgaaaatga gcaatgaaag ttacgtaaag aagtgatgaa gtacattaat
    10441 aaggaatcgg ctgacattga taaggaaata aacaagttaa tggcattaat tgaacgcact
    10501 acgacagtta atgacattac agaactacgt gatggtttag aagattttat tgcgaattat
    10561 aattcaattt caaccattaa tggatcgatt gtcacacaaa ataagacatt agatttagct
    10621 gacttgatac cagcaaggca cgtgaaaaga atacatcatg ttgaaaatat tgatgaagct
    10681 attgctaaag caagtgatgt gttagttgct aatcatttta ttgatattaa atatattcat
    10741 gagatgcaac aggcatttga tgattcgtat atggttatca tgcaaaatat tgctattcca
    10801 catgcatact ctgaaaagca tgtacataaa acagcgatga gtatgttgat attacaagaa
    10861 ccaatataca tgtcagatgg cacagcaatc catattattg tacctattgc tgctgttgat
    10921 aaagtgacac acttaagagc gttactacaa ttgagagatg tggcgcaaaa caatgacgca
    10981 attaagcgca tcatacaaag tcgcaaaaat tctgatgtaa atgagatttt aaaaaattat
    11041 tcaaataaag aagcgaggga aaatggatgg gacagcaatt agtgcataaa gaaaatataa
    11101 tgctcaattt gtcggcaact gataaagaat ctgtattgtc acaaatgtca gatgtgttat
    11161 ttcaaaatgg gttcgtaaag tcaacgttta aagatgcagt catcgacaga gaaaaagagt
    11221 ttgctactgg tttaccaacg catctatgtt cggttgctat accgcataca gatgtcgaac
    11281 atattaacca tagaacgata ggtgtggctg ttctagaaaa agaagtgccg tttattgaaa
    11341 tgggaacact tgatcaacag acagaagtga aaatcgtttt tatgttagca atggataaag
    11401 tagatgatca acttaagctg ttacaacagt tgatgcaaat ttttcaaagt gaagaaaaat
    11461 tggagcagat tctacgaacg aaagatgaaa caattttagc aacactaatc aatgattatt
    11521 tgaaatataa ctaaaaatta attggaggaa ttgaaaatga aacaagtatt agtagcgtgt
    11581 ggtgcaggta ttgcaacgtc aacagtagta aataatgcaa ttgaggaaat ggcaaaggaa
    11641 cacaatatta aagtagatat taaacaaatc aaaattacag aagttggacc ttatgaagac
    11701 actgcagatt tattagttac aactgcaatg acaaagaaag aatataaatt cccagttatc
    11761 aacgcacgta atttcttaac tggtattggt attgaagaaa caaaacaaca aatcttaaca
    11821 gagttacaaa aataactgaa ttgatatgta acgtgggtca ataacatata aatatatgca
    11881 aattgcatat aaatgactat cacgacaaat gattgtagat gacatagaaa cgcagtgact
    11941 gtaattcaaa gggattgcag tcgaatgtag caagggttgt cgttgaaatg actgtaacgt
    12001 caaatgtaat cacaatcgga aagtatcgtg acaatgcata tataacaggg agggtttaaa
    12061 tatgagttac ttcactgatt ttgtaagggg atttttagat ttaggtgcaa ctgttatttt
    12121 accggttgtc atattcttgc ttggcctatt ctttaggcag aaaattggag cggcatttag
    12181 gtctggttta acaataggtg tggcttttgt agggattttc ttagtcatcg atttattagt
    12241 taaaaattta gggccagcag cacaagcgat ggttaaaaat ttaggcgtca gtctgaatgt
    12301 gattgatgta ggttggccag caacatcatc tatcgcgtgg gcatcatctg tcgcagcatt
    12361 tattattcca ctcggaatca ttgttaacgt tgtattgcta gtaactaaag tgacaaagac
    12421 gatgaatgta gatatttgga atttttggca ttatacgttt acagcagcaa tggtttatgc
    12481 cgtatcaggc agtatttggc aagcgttatt agcagcagtt attttccaag ttatctgttt
    12541 gaaagtagca gattggacag caccgatgat gagtgagttc tttgatttac caggtgtatc
    12601 gattgctaca ggaagtacaa tttcttatgc accaggtatt tacttagtta aattgttaca
    12661 aagagtaccc ggtctgaata agttagatgc tgatcctgaa acaattcaaa aacgttttgg
    12721 cgcatttgga gagtctatct ttgtcggatt aattttaggt ttaggtattg gtgtgttagc
    12781 aggttacaaa cctggagaca tcattaattt aggaatgtca atggctgcag taatggtatt
    12841 aatgcctaga atggtaaaaa tcttaatgga aggtttaatg ccagtttcag agtctgcaag
    12901 aacatggcta aataaacgtt ttggcgaacg tgaaatttat attggattgg atgcggctgt
    12961 agcattaggt catccagcgg ttatttcgac agcattaatt ttagtaccta tcactgtttt
    13021 attagccgtt attttaccag gaaaccaagt attacctttt ggtgacttag caactatacc
    13081 atttgttgtc gcgtttattg ttggtgcagc aagaggaaac attattcatt ctgtcattgt
    13141 gggtacgatt atgattgcaa tttcactata tattgcaaca gacgtagcac ctattttcac
    13201 agatatggcg aaaggtacga atgtacaaat gccaaaagat tcatctgaaa tttcaagtat
    13261 tgatcaaggt ggtaatatcg ttaactatct tatctttaaa ctatttagtc tattcaatta
    13321 aaaatcgagg tgtttgtggt gaaagcttta gtaaaaacaa gagaaggaca tggcaactta
    13381 gaacttcttg ataaagaagt tgcaacaccg ctagatgata aagtaaagat taaagtacat
    13441 tatgcaggaa tttgtggcac agatattcat acttatgaag gtcattataa agttaatttt
    13501 ccagtgacat taggtcatga attttctggt gaaatcgttg aagttggagc agacgttaaa
    13561 gattttaaag ttggtgaccg tgtcacttct gaaacgacat tctatgtttg taatgagtgt
    13621 gaatactgtg aatcaaaaga ctataattta tgcaaccatc gaaaaggtat tggaacacaa
    13681 gttgatggcg catttactaa ttatgtcatt gcacgtgaag aaagtttgca tcatattcca
    13741 gacgaagtgt cgtatcagtc tgcagctatg acagaaccat tagcatgtgc acatcatggc
    13801 gtttctaaga ttcaagtcaa ttcaggcgat gtagcagttg taatgggacc tgggccaatc
    13861 ggattacttg tagcacaagt gttgaaaagt aaaggcgcaa ctgttgtggt aactggattg
    13921 gacaatgaca aagtcagatt agataaagca gaagcattgc acatggatta tgtagtcaat
    13981 ttacaacaaa cagacttaaa aacgtatatc aatggaatta cagacggata cggtgcagat
    14041 gttgttgttg aatgttcagg tgcagttcca gcagcacgac aaggtttgga tattttacgc
    14101 aaaaaaggtt tctacagtca aataggtatt tttaaggatg ctgaaattac atttgatatg
    14161 gaaaaagtga ttcaaaaaga aataacagtt gttggtagta gaagtcaaaa gccggcagat
    14221 tgggaacctt cattgcaact tatggcggat ggtttagtaa atgctgaagc tttggtgaca
    14281 aaaatatatg atatttcgaa atgggacgag gcgtatcaac atttaaaatc tggcgaaggt
    14341 attaaagcat tacttaagcc gctcgattta gatgaaaatg aaggagagaa ttaatatggt
    14401 agaatcaatg ctaactttta tgcttgggcc attgagacaa atcactgatt tttatatgga
    14461 acatctactt gtaagtaatt ctatcgtcat tgcaggttat tttgcgacag gtatttttaa
    14521 aaagaaaaaa gttgtgaatt aaatcaaagt tgaggtgatt tacaagtgaa agctttgaaa
    14581 ttatatggcg tggaagattt acggtatgag gataatgaaa agccagtcat tgaaagtgcg
    14641 aatgacgtta ttgttaaagt acgagcgact ggaatatgtg gttcagacac gtcacgatac
    14701 aaaaaaatgg ggccatacat tcaaggtatg ccatttggtc atgaattttc aggtgttgta
    14761 gatgccattg gaagtgatgt tacgcatgtt aatgtaggcg acaaagtgac aggttgccca
    14821 gccatacctt gttatcaatg cgagtattgt ttaaaaggtg aatatgcacg atgtgaaaag
    14881 ttgttcgtca ttggctcata tgaacctgga tcgttcgcgg aatatgtcaa attgccagcg
    14941 caaaatgttt taaaggtacc agacaatgtt gattacattg aagcagcaat ggttgagcca
    15001 tcagccgttg ttgcgcatgg gttttataaa tcgaatatac aacctggtat gactgttgca
    15061 gtaatggggt gtggcagtat aggattgtta gctattcaat gggcacgaat atttggtgct
    15121 gcgcatatca tcgctataga tatagatgcg cataaactag atattgctac atcattgggc
    15181 gcacatcaaa ccatcaattc aaaagaagaa aatcttgaga aattcatcga aaatcattac
    15241 gccaatcaaa tcgatatagc tatagaatca tcaggtgcta aagttacgat tggtcaaata
    15301 ttgacgctac ctaaaaaagg tggcgaggtg gtattactcg gaataccata tgatgatatt
    15361 gagattgatc gcgttcattt tgaaaaaatt ctgcgtaacg agttgacagt atgtggctct
    15421 tggaactgtt tgtccagtaa ttttccgggg aaagagtgga cggcaacctt acattatatg
    15481 aagacgaaag atattaatgt aaagcctatt atttctcatt ttttaccgtt agaaaaaggc
    15541 ccggagacat ttgataaatt agttaacaag aaagaacgat ttgataaagt catgtttacg
    15601 atttattagt atgcaccttt gaggacgaaa acgctggtat agttatagct atgaaagtgc
    15661 gaatgccgtc tggtctacag atactatcga aataattcat cttcgaatat acgttgctaa
    15721 atagccggtt tacttgtgtg aaatatgctt gtgaatcggt tgttttgcat tttgtatact
    15781 taaaatgaga tggcaatatt tgataatttt taaagtgaaa atcaagtaca gccacttaat
    15841 aagataaatt tattataata tatggtaaaa tgatggcagt aataatgaat ttgaaaaaga
    15901 gtaaacatta atacctttaa caatttaata tcgtcagtgt taatgattaa ctgcatggca
    15961 aaacaactta gaatggtcag ttacaaaaat acatttttat aaaaaattat cacactattg
    16021 tgacaactgc ctttggctta ataaaagagg aaagtgagca ataggttggg cttatgtgcg
    16081 ggcataggtc agtaatgtat aaatggaaat gatgtaatga cagaatggag gacaacatga
    16141 tttatgcagg tattttagca ggaggtattg gttcgagaat gggaaacgtg ccattaccaa
    16201 aacaattttt agatattgat aataaaccga ttttaattca tacaattgag aagttcattt
    16261 tagtgagtga atttaatgag attattatcg caacgccagc acagtggatt tcgcatacgc
    16321 aagatatttt aaaaaaatat aacattacag atcaacgtgt caaagtagtt gcaggtggta
    16381 cggatcgaaa cgaaacaatt atgaacatta tcgaccatat tcgcaatacg cagggtatca
    16441 acgatgatga tgtgattgta actcatgatg ccgtaagacc atttttaact caacgtatta
    16501 ttaaagagaa cattgaagta gcagcaaaat atggtgcagt agatacagtc attgaagcaa
    16561 ttgatacgat tgtaatgtct aaagataaac agaacataca cagtatccct gtaaggaatg
    16621 aaatgtatca aggccaaaca ccacaatcat ttaatattaa attattacaa gatagttatc
    16681 gtgccttaag tagtgcacaa aaagaaatct tatcagatgc atgtaaaatc attgtcgaat
    16741 ctggacatgc agttaaattg gtacgcggag aactatacaa cattaaagtg acaacaccgt
    16801 atgatttaaa agtagcaaat gccattattc aaggtgatat tgccgatgat taatcaagta
    16861 tatcaactcg ttgcaccgag acagttcgac gtcacatata ataatgttga tatttatggt
    16921 aatcatgtca tcgtaagacc tttatacttg tctatttgtg cagctgatca aaggtattac
    16981 acaggtcgaa gagatgaaaa tgtactgcgc aaaaaattac caatgtcatt agttcatgaa
    17041 gctgttggtg aagttgtatt cgatagtaaa ggcgtatttg aaaaaggtac gaaagtagta
    17101 atggtgccga atacacctac agaaaaacac gacgtgattg cggagaatta cttagcctct
    17161 agttatttta gatctagtgg ttatgatggt tttatgcaag actacgttgt gatggcacat
    17221 gatcgtatcg ttccgctgcc taatgacatt gatttgagta cgatttcata cacagagtta
    17281 gtgtcagtaa gttatcatgc tatacaacga tttgaacgta aatctatacc tttgaaaacc
    17341 agctttggta tttggggtga tggtaactta ggttatatta ctgctatttt gctgcgtaag
    17401 ttgtacccag aagctaaaat ttatgtattt ggtaagacag actataaatt aagtcatttt
    17461 tcatttgtag atgacatctt tacagtaaat caaataccag atgatcttaa aattgatcat
    17521 gcatttgaat gtgttggagg taaaggaagt caagttgcac ttcaacaaat agttgaacat
    17581 atttcaccag aaggcagtat tgctttgtta ggcgtaagtg aattacccgt ggaagtgaat
    17641 acacgattag tacttgaaaa aggattaacg ttgattggta gtagtcgaag cggctctaaa
    17701 gattttgagc aagttgttga tttatatcgt aagtacccag acattgttga aaagttagcc
    17761 ttattaaaag gacatgaaat taatgtatgt acgatgcaag atatcgtcca agcgtttgaa
    17821 atggatttat cgacatcttg gggaaaaaca gtattgaaat ggacgattta ataaaccgat
    17881 gggggaataa caatgacaaa aacgaaacaa gcaatacata ttgataacat atactgggaa
    17941 cgtgttcagt tatatattga aggacatagt gaaggcgtag atttaacatc aggacaattt
    18001 gttctgagga atttaaccga aacaaaaaca ttagaagcaa atgaaatgaa aatagacggt
    18061 aatacatttc tatgtagatt caatgtcgca atattagacg atgggtatta tttaccaatg
    18121 gataaatatt tatttgttta tcatgaccag ttagagtata ttggacaact taatccaaat
    18181 attattgatc aagcttatgc ggcattaaat gaagagcaaa ttgaagaata caatgagctg
    18241 actacacaaa atggaaaagc aaactatgtg ttagccaacg aagctaaagt tttccgcaaa
    18301 ggtggcgtat cacaacatac ggtttatacc attactccag aaatagcaag tgacgttaac
    18361 gaatttgtat ttgatattga catcacctta cctcaagaga aatcaggggt cattgcgaca
    18421 agcgcacact ggcttcataa acaaggccat aaagcttcat ttgaaagtag aagtttctta
    18481 tttaaagcta tttttaatat tacaaagtta ctacatatta aaagaagcaa aacaatatta
    18541 ttcacatcag attcacgtcc gaatttatca ggtaacttta aatatgtata tgatgagtta
    18601 ctacgccaaa aagtagattt tgattatgat attaaaacgg tatttaaggc gaatattacg
    18661 gatagacgta aatggagaga caagtttaga ttgccatatt tacttggtaa ggctgattat
    18721 atttttgtcg atgatttcca tccattaatt tatacggttc gctttagacc atcacaagaa
    18781 attattcaag tgtggcatgc cgttggtgct tttaaaacag ttggctttag tcgtacaggt
    18841 aaaaaaggtg gtccgtttat tgattcatta aatcatcgta gctatacaaa agcttatgta
    18901 tcatcagaaa ccgatattcc attttatgct gaagcatttg gaattagaga agaaaatgtt
    18961 gtaccaacag gtgtaccacg tactgatgta ctatttgatg aagcttatgc aacacaaatt
    19021 aaacaagaga tggaaaatga attaccactt ataaaaggta agaaagttat tctattcgca
    19081 ccgacattta gaggtaatgg tcacggtacg gcacattatc cattttttaa aattgatttt
    19141 gaacgtttag caagatattg cgagaagcat aatgcagttg tgttattcaa aatgcatccg
    19201 ttcgtaaaaa atagactcaa tatttcacgt gaacatagac aatactttat cgatgtgtca
    19261 gattatcgtg aagttaacga tattctcttt gttacagact tgttgattag tgattattca
    19321 tctttaatat atgaatatgc agtatttaaa aaaccaatga ttttctatgc atttgactta
    19381 gaagattata ttacgacgcg tgatttctat gaaccatatg aatcatttgt tccaggtaaa
    19441 attgtacaat cctttgatgc attaatggat gctttggaca atgaagatta tgaggttgaa
    19501 aaagttgtac cattcttaga taaacatttt aaatatcaag atggtcgctc aagtgaacgt
    19561 ttagtcaaag atttgtttag acgctaatgt tggcatatat tacttgccat gcaaatgatc
    19621 ataaggcata gttttcaaga agggttatga caatgaagtg agcaacatgt catgatgaga
    19681 agcaggacta tacaatgaga ataacctttt gatttttcat gcataagagg ctgggacata
    19741 aatccctaaa aaaacagcag taagataatt ttcaattaga aaatatctta ctgctgttct
    19801 ctatttatac aatacttcgt attgaatggc ttcgctttgc ccgtctggca cattactgta
    19861 aaattctata aatag
//
LOCUS       DAMZPP010000037        19720 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMZPP010000037 DAMZPP010000000
VERSION     DAMZPP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19720
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.037"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            122..1393
                     /gene="sucB"
                     /locus_tag="POH73_001025"
     CDS             122..1393
                     /gene="sucB"
                     /locus_tag="POH73_001025"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDI6886992.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNLGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAVIGEGSGNASKENSNDNTPQQNDETTNNK
                     KEETTNKSADKAEVNQTNDDNQQRVNATPSARRYARENGVNLAEVSPKTNDVVRKEDI
                     DKKQQAPASTQTTQQAPAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAML
                     TTFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGD
                     DMITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMV
                     NGSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSY
                     DHRIIDGKEAVGFLKTIKELIENPEDLLLES"
     gene            1983..2792
                     /locus_tag="POH73_001026"
     CDS             1983..2792
                     /locus_tag="POH73_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6886993.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNCEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSSKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKIGTGGLGGEIHLMPVETEMTMPAYGAVDQV
                     EFETKDVDFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
     gene            2821..3024
                     /locus_tag="POH73_001027"
     CDS             2821..3024
                     /locus_tag="POH73_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6886994.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            3205..3996
                     /locus_tag="POH73_001028"
     CDS             3205..3996
                     /locus_tag="POH73_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDI6886995.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQDDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            4010..5896
                     /locus_tag="POH73_001029"
     CDS             4010..5896
                     /locus_tag="POH73_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HDI6886996.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPEFEDLKRTDASQVDGQDDTSEDDDNESEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDDHVQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLL
                     LKSL"
     gene            6121..7464
                     /gene="brnQ"
                     /locus_tag="POH73_001030"
     CDS             6121..7464
                     /gene="brnQ"
                     /locus_tag="POH73_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDI6886997.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAIALIFIYISLGYIGNHMPVSDMKLNELKSHDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRITQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
     gene            complement(7532..8668)
                     /locus_tag="POH73_001031"
     CDS             complement(7532..8668)
                     /locus_tag="POH73_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDI6886998.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVAPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(8700..9329)
                     /locus_tag="POH73_001032"
     CDS             complement(8700..9329)
                     /locus_tag="POH73_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDI6886999.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGIIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(9348..9617)
                     /locus_tag="POH73_001033"
     CDS             complement(9348..9617)
                     /locus_tag="POH73_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDI6887000.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            9779..10087
                     /locus_tag="POH73_001034"
     CDS             9779..10087
                     /locus_tag="POH73_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDI6887001.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVLQNNEVYYKLPIID
                     "
     gene            10258..10458
                     /gene="cspA"
                     /locus_tag="POH73_001035"
     CDS             10258..10458
                     /gene="cspA"
                     /locus_tag="POH73_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HDI6887002.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
     gene            10655..11056
                     /gene="msa"
                     /locus_tag="POH73_001036"
     CDS             10655..11056
                     /gene="msa"
                     /locus_tag="POH73_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HDI6887003.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILAAMMIVLVIPIMISGI
                     VFFKTNIDKTYIFFNIIFIDFYYYIYNVHLMTLPKFNNYIKTEMMELEHIDVLITSKD
                     FGFDEILFFTLYLLLILIVLYYLKKQLKNKT"
     gene            complement(11292..12558)
                     /gene="lysA"
                     /locus_tag="POH73_001037"
                     /pseudo
     CDS             complement(11292..12558)
                     /gene="lysA"
                     /locus_tag="POH73_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
     gene            complement(12548..13633)
                     /locus_tag="POH73_001038"
     CDS             complement(12548..13633)
                     /locus_tag="POH73_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDI6887004.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHEIVQSLTVKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            complement(13638..14789)
                     /locus_tag="POH73_001039"
     CDS             complement(13638..14789)
                     /locus_tag="POH73_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDI6887005.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTTLM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSYLNFDEKVLINVVNFYENLLNNYKEV"
     gene            complement(14932..15651)
                     /gene="dapD"
                     /locus_tag="POH73_001040"
     CDS             complement(14932..15651)
                     /gene="dapD"
                     /locus_tag="POH73_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDI6887006.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFGDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            complement(15678..16400)
                     /locus_tag="POH73_001041"
     CDS             complement(15678..16400)
                     /locus_tag="POH73_001041"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDI6887007.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKDADVAIDFSNPNLLFPLLDEAFHLPLVVATTGEKEKLLNKLDELSKNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            complement(16397..17284)
                     /locus_tag="POH73_001042"
     CDS             complement(16397..17284)
                     /locus_tag="POH73_001042"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDI6887008.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNLEALKAHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKELILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLREAYDTFKAGENE"
     gene            complement(17286..18275)
                     /locus_tag="POH73_001043"
     CDS             complement(17286..18275)
                     /locus_tag="POH73_001043"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDI6887009.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGKEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKGGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLEKEATAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            complement(18339..19544)
                     /locus_tag="POH73_001044"
     CDS             complement(18339..19544)
                     /locus_tag="POH73_001044"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDI6887010.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKTPKQQELALLLTTGEQQTVSYLSMVLNDIGVNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINDHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIMSNEEILERKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILKTLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
BASE COUNT         6514 a         3236 c         3195 g         6775 t
ORIGIN      
        1 ttaagttatc atggcagaat tcaaagggct gctccagctg aaggcgatgg agaaattcat
       61 aaacttgttc aaaataaaat tatagaaaat gcattaaaaa ataactaggg ggaaataagt
      121 catgccagag gttaaagttc cagaattagc agaatctatt acagaaggta ccattgcaga
      181 atggttgaaa aacctagggg atagcgtaga aaaaggtgaa gctattcttg aattagaaac
      241 tgataaagtt aatgtcgaag ttgtatctga agaagcaggt gtattatctg aacaacttgc
      301 aagtgaaggc gacactgtag aagttggaca agcaattgct gtcatcggcg aaggtagtgg
      361 caatgcttct aaagaaaata gtaacgacaa tactccacaa caaaatgatg aaacaacaaa
      421 taataaaaaa gaagaaacaa caaataaatc ggcagataaa gctgaagtca atcaaacaaa
      481 tgatgacaat cagcagcgtg ttaatgctac accttctgcg cgtcgatatg ctcgtgaaaa
      541 tggtgtgaat cttgctgaag taagtccgaa aacaaatgat gttgttcgta aagaagatat
      601 tgataagaaa caacaggcac ctgcatcaac acaaacaaca caacaagcac ctgcaaaaga
      661 agagaaaaaa tacaatcaat atcctacaaa accagtgatt cgtgaaaaaa tgtcacgcag
      721 aaagaaaaca gctgctaaaa aattattaga ggtatctaat aatacagcta tgctaacaac
      781 atttaacgaa gttgacatga caaatgttat ggaattgcgt aaacgtaaga aagaacaatt
      841 tatgaaagat catgatggta ctaaattagg atttatgtca ttctttacta aagcttctgt
      901 agcagctttg aaaaagtatc cagaagttaa tgcagaaatc gacggcgacg acatgattac
      961 gaaacaatat tatgatattg gtgtagctgt ttcgacagat gatggactat tagtaccatt
     1021 cgtaagagat tgtgataaaa agaattttgc agaaatcgaa gcagaaattg ctaatttagc
     1081 tgttaaagca cgagagaaaa aacttggctt agatgatatg gttaatggtt catttacgat
     1141 tacaaatggc ggtatttttg gatcaatgat gagtacgcca attatcaatg gtaatcaagc
     1201 tgcaatctta ggcatgcatt caattattac aagaccaatt gcgattgatc aagatacaat
     1261 cgaaaatcgt ccaatgatgt atattgcatt aagctatgat catagaatta ttgacggtaa
     1321 agaagcagtt ggattcttaa aaacaattaa agaattaatt gaaaacccag aagacttatt
     1381 attagaatct taatccacaa cacaaaatag tttactattc ctaaaaacgg gatcaaatca
     1441 ataacaaaca gcagtaagtt tattttctag tcgaaatatc ttactgctgt actttatttt
     1501 tacaatgctt cgtattaaaa tgtgagttta attttgacta cttaaaaaat atatcatttc
     1561 tatatgaaat agactcacga tagaaatata gctagaatag cgtgtttcaa tatgctaatg
     1621 aaatcatttt agataataac aagcgatgaa atttatgaat tctataagcg cctttagtta
     1681 atttaaaatc atctgaacaa gatgtacatt cactttatgg acacgacata tatatgtttt
     1741 tcatgtacta cagaaaaacg aaatatccca atatccaatc gctatataca gatactcatt
     1801 aatgacatac tcaatagcaa ccataacaat attatgttag ctatttaaat gaaatagttc
     1861 ttttaaagaa atacattttt cacatattaa tctataatca aaatcaactg accgatattc
     1921 tataatttat gattaaaata agttataata taatagtaaa gataaagata gaggtggcta
     1981 taatgtgtgg acttagaagt ataacattag gtacaacaaa tatagaacag acaaaacatt
     2041 tcatggttga catattagga ttaaattgtg aagaacttct tgaaaactca attcgtttcg
     2101 gcgatgcaga tataagccca ggaacaagac ttcaatttat acaagttcca agtgagcaat
     2161 tagaagaatc tcactttgtg ggtattggat tacgtacacc aactgactca ggtctagagg
     2221 agtatgcgga aatattatcg agtaaggata ttccatttac aacagttaaa gaattaaatg
     2281 gcaataaata tttcagtctc gaagataaca atggtcatat tttctcaata tattcaaacg
     2341 agaataatta tggcgttggt ttaggtatgc cttctttcga aagtgcggtc aatccgttac
     2401 atcaagtgca aggtttaggg ccagtgattc ttaaagtgaa tcatgtagat attacaggtc
     2461 aaattttaac aaatatattc ggacttgaag tatttgcaga ataccaaccc ttcgacaatg
     2521 ctgactatca tgtccaagta ttcaaaattg gaacgggtgg tctaggtggc gaaatacatt
     2581 taatgcctgt agaaaccgaa atgacaatgc cggcatatgg cgcagtcgat caagttgagt
     2641 ttgaaacgaa agatgtagat ttctttaatc aagcgaaatc gcgcttagat gaagtggaaa
     2701 taccatatca aacacttgag caagatgata ttgaatcaat tagaattact gaaaacagtg
     2761 gattatcgtt tatattcact ttacaaaaat aattttttta cgatagtgag gacaaaattt
     2821 atgttacatg aaacttggaa agaacgtacg ccaatcaaga aagtagaagt cattaataca
     2881 gatgcaaaga aatttacagt ttctgacatg ttaactgtag gcaaacaata tgatgttatc
     2941 aacgaaactg aagaatatta tcaaataata gataattcag gattagttgg cggttactat
     3001 aaaacatatt tcaaagaagt ttaaacgcat aattattgaa atgaatccta atatgagatt
     3061 ttcaattagt aaatttggta aaatataaac aattcgcata ctaagcattg cgttttatat
     3121 cagaatagaa tagtaaacta aggcatttgg agaacttatt ccatgtctta gttttttaat
     3181 ttatttaaag gagaacgttt taaaatggca ctaaaacatt ataagaattc agattcaaca
     3241 gttttcaatg atgcgaaggc attattcgat ttaaataaaa atattttact taaaggtcca
     3301 acaggttcag ggaaaacgaa gttggcagaa acattaagtg aagttgttga tacacccatg
     3361 catcaagtca actgttctgt tgatttagat acagaaagct tattaggctt taaaacaatt
     3421 aaaacaaatg cggaaggaca acaagaaatt gtctttgtag atggtccagt tattaaagct
     3481 atgaaagagg ggcatatttt atatattgat gaaataaata tggctaaacc tgaaacatta
     3541 cctgtattaa atggggtctt agattatcgt cgtcaaatta cgaatccata cactggtgaa
     3601 gtaatcaaag ctgtaccagg atttaacgtt attgcagcga taaatgaagg ttatgttggt
     3661 acattgccaa tgaatgaagc actaaaaaat cgctttgttg ttattcacgt tgactatatt
     3721 gacggggaca ttttaaaaaa tgtaattaaa gagcaaagtt tattacaaga tgataaacaa
     3781 atcgaacaaa ttattaagtt taacgaagat ttacgtacta tgtctaagca gggacaaatt
     3841 tctgaagaag ccgctagtat acgtgcatta ttagacttgt gtgatttaat cactgtaatg
     3901 ccagttgaac gtgcaattaa acgtacaatt attgataaat tggaagatga acgtgaacaa
     3961 caagcaatat ataatgctgt agaactaaac ttttaaagag ggataaaaca tgagtgatcg
     4021 tttcataaaa tttaacgacg aacagttaga tgccaagcaa gtaatgatgt tgcaagactt
     4081 agctcgactc ttattaaaaa atgaacaaac acaagttaaa atccaaaaat tcccttacta
     4141 taatcctgtt caaaatgttt tgattacgag ttggttttgg tcacatcgac caagccatat
     4201 cgaaatggct ggtttaaaaa cagatgttat gttagctgct tacggttatc atatgatgga
     4261 tgtacaaatt gtaaatgaag tagtacaaga taaaactttt aaacacccta aattttatca
     4321 acaacttttt aaattattag aagatatgcg cgtattaaat tctattaaag ttgagcgccc
     4381 aagtactgct aaattaattg atcttcgctt agatacacgg atatcatata cggaatctca
     4441 aattaaagtg tatcgtacta aaacacaata tacagattta ttatttttgt atttagaaca
     4501 tgcattttta agtcaagatt tctttgatat accatctata cattcagatt tagatgatat
     4561 tttagttaat atgttcttat atttacctaa ttttttccaa aatcaaaatt ctgaagataa
     4621 tatgtatctg gctcaaagaa ttatgtatca agtagatgat attttaaaag aagatatgct
     4681 aaacgaatat tattacttac ctaaaacact atataataca ttagctagtc cagagtttga
     4741 agatttaaag agaactgatg caagtcaagt cgatgggcaa gatgatactt cagaagatga
     4801 tgataacgaa agtgaaaaag ctgactctaa atctgcagat agtgagtcaa aaggtggcgc
     4861 ctatttagaa atggagcttc acgaaggaca aaatagtgaa acattaggta atgatgaagc
     4921 acgtgaaggc gatgctacag acgatatgac tgatatgatg accaaaaagg gcaaaggatc
     4981 aaacgatacg ctaaatcgtg aagaaggcga tgctgttggt caaagccaag catttcaact
     5041 tgatggtgtg aacaaaaatg tagaaataaa atggcaaatt cctgaaattg agccacaata
     5101 tgttttagaa tatcaagaat cgaaacaaga cgtacaatat gaaatcaaag acttaattca
     5161 aattataaag aaaacgattg aacgagaaca acgagatgca cgttttaatt taacaaaagg
     5221 gcgattacaa aaggatttaa ttaattggtt tatcgacgat cagtataaat tgttttacaa
     5281 aaaacaagat ttaagtaaat catttgatgc gacgtttaca ttgttaattg atgcgtcagc
     5341 aagtatgcat gataaaatgg ctgaaacgaa gaaaggtgta gtgttattcc acgagacgtt
     5401 aaaagcattg aacattaaac atgaaatttt atcattcagt gaggatgcct ttgattcaga
     5461 cgatcatgta caaccaaata tcattaatga aattattaac tatgattact caacctttga
     5521 aaaagatggc ccacgtatta tggcacttga acctcaagat gataatcgtg atggtgttgc
     5581 tattagagtt gcaagtgaac gattaatgcg tcgaaatcaa catcaacgat ttttaatcgt
     5641 attttctgac ggcgaaccgt ctgcatttaa ttatagtcaa gatggtatta ttgatacgta
     5701 tgaagctgta gaaatgtcac gtaaatttgg cattgaagta tttaatgtat ttttgagtca
     5761 agatccaata actgaagatg ttgaacaaac aattcataat atttatggtc aatatgccat
     5821 tttcgttgaa ggtgttgcgc atttacctgg acatctctct ccattattaa aaaaattact
     5881 acttaaatct ttataaataa gtttaaaata tgagtttata tgaaattgtg aataaaagtc
     5941 atcagttact atttaaaaag taaatttgaa gaattttatt ttctggcata atttgattta
     6001 aacaacacat gtgttttgtt tctgctatgt acaacatatt ccatatagac tcttgattat
     6061 tttttgtgct aaaatattaa cttgtaaatt atttcgtgtt ttaaaaaaag gagtaactct
     6121 atgaataaaa atacatgggt cattgggttt acgctcttcg ctatgttttt tggcgcaggc
     6181 aatctaattt ttcctcctaa tctcggattg gatagtggtc aatttttctg gcccgccatt
     6241 ttagcgtttg ttctaactgg gattggttta ccattattag gtgtgattgt aggtgcactt
     6301 gataaagaag gatatattgg cgcattaaat aaaatttcac ctaaattttc aatattgttc
     6361 ttaatcatca tttatttaac tataggacca cttttcgcaa tacctagaac tgcatctaca
     6421 tcttttgaaa tgacaattac accaattata catagtaata gtagtatcgc tttatttata
     6481 tttacgatta tctacttcat agtcgttttg tatatttgtt taaatccatc taagttaatc
     6541 gatcgtattg gttcattatt aacaccatta ttattgatta ctattttagc gatgattatt
     6601 aaaggatact tagactttag cggtaatagt gctggaaagg gcaatgaagc actatatcat
     6661 tctaattttt caagttttgc tgaaggcttt acacaaggct atttaacaat ggatgccatt
     6721 gcagcaattg ctttttcaat gattgttgtt aatgcagtaa aactaacagg tattactaaa
     6781 acaaatcaaa tattcaaaca aactttgact gctggtttaa ttgcagcaat tgctttaatt
     6841 ttcatatata tttcattagg ttatattggt aatcatatgc cagtaagtga catgaaacta
     6901 aatgaattga aatcacatga ccgtaacatt gggacatatt tattaacgac aatggcttca
     6961 acaggatttg gttcattcgg aaagtattta ttgggcatca ttgtggcgct ggcatgtcta
     7021 actacagcat gtgggcttat tgttgcagtt tctgaatatt tccatagaat cgtacctaaa
     7081 gtatcataca aagcatttgt attagttttc attttaatga gttttattat tgctaaccaa
     7141 ggtttaaatg ctgttatctc aatgtcaatt ccggtattaa gcattgtata cccagtagca
     7201 ataactgttg tattattaat tttaattgcc aaattcatac cgacaaaacg cattacacaa
     7261 caaattccag ttattatcgt atttatattg tcgattttca gtgttattag taagttaggt
     7321 tggctgaaaa ttaactttat agaatcattg cctctaagag cgtattcttt agagtggttc
     7381 ccagtagcaa ttattgcaac gatattaggc tatctagtcg gcatatttgt aaaacaagat
     7441 ccaattaaat atcaacagga ataacgaaaa aaggaagcat ctccaattta atcgtaggat
     7501 gcttcctttt ttgttatatc taattcgtaa tttatgattg tttattcggt cctttgatgt
     7561 tcactaaatg tgactttaaa tcttgttcta attgttgtaa ttctttttca gctaattgtc
     7621 gttcttcgcg accgtgttgt tgaataatta atgtttcttc aattgtctca ataatgttac
     7681 gctgtgtacg tttcaatgta tcaaggtcaa caatgccacg ctcattttct gttgcagttt
     7741 caatcgcatt ttgtttcaac atttcagcat ttgctgttaa taaatcatta gttgtatcag
     7801 tgacagctcg ttgtgcagca actgcattac gctgtctcat taatgtaagc gcaatggcca
     7861 tttgattttt ccatagtgga atacttgtca aaattgaact ttgtatcttc tcggcaaggg
     7921 cttgattaac attttgaatc atacgaattt gtggcgcagt ttgcaacgct atttgtcttg
     7981 aaagctgtaa gtcatatatg cgtttatcta gtctatctat aaattgctgc atatctgcaa
     8041 cttgttgaat atccatttga ttagtggatt gctgcgcttg ctgttgcaat tgtggtagct
     8101 tttcattttc taattgcaac tttttttgct gtgcagcaat gatatgcaat gataagtcat
     8161 caaagtattg tttgttttta tcatatagcg tatctaataa ttcaatatct cttgttaaat
     8221 gtgtttgatg tttctgcagt tgaatcgtta tgcgatcgac ttgagcacta actgattgca
     8281 ttcttgaaaa gatttcattg atagacgact ttgctctgct aaaaattctt tttaacatag
     8341 atggtttatc agtatttaac tcatttggat taactgactt tagttttgac attagatctg
     8401 acaaagtatc tccaatagga gcaacatctt tactttgtac ctcatccaac atttgatgtg
     8461 aaaattgaga catttgtttc tgtaaatcag aaccaaacgc taataaacct tcattgtcta
     8521 aagggttaat ttgtttactg attgtgtcta cctgtttttg ttgttcaatt gttaactttt
     8581 ctttttgaat agtagtattc gataattctt tatcgcttat aaaatcatct agtggatggc
     8641 tttctttgaa acttttattt tcagtcatca atcatctctc caatagttgc tattttacat
     8701 caatcttgat gatgcttttg atgtaactta tttaattcca tttcaatatc taaacgttca
     8761 taatcatctt cgttgagacg ctttaaatca gcgattaatg ttcgtttgac ctcatccaaa
     8821 gtaattcgtg tttgttctaa tttttgctgt tcattaattg attttttggg catttttgct
     8881 agacgtgtat atgcatcaac caaatttaaa gcattatcaa tatgagaata aaaaaagccc
     8941 tcaactttat aaaatgatgc aggtctctgt ctaactgtcg tataaataga acgtgaaatt
     9001 tggtatatat cattaatttg cctaaaatct ttaattgatc ttatattgac atacgttttt
     9061 aaaatacctc taagtttttg gtgtgtatga tttaactgat ttcgaatata gcgatagtct
     9121 tttctagtca aaccaatttc gtttaaatat ttgcgtgaag tgagtttttg tatcggtagg
     9181 tatgtcatta aaaagccaac aataccaata gacatatcaa ttaaaaaaga tacatcaagt
     9241 gcaattatcc caaatatgct tgttaaaaac gctacaggaa ttcccactaa caccccaaat
     9301 atatgagaaa tattatatct cactgtcatc ttcctttatt tagcatttta tattgatcga
     9361 aaatccgata atttttgatt tagttctaac tcttcaagtt gatggctcgt tacatttgat
     9421 gctggtgagg caccttcaat tacaccttga ataaatctct ctatatctgc gtcatcccct
     9481 tgtgcatata tctctacata gtcatctaca ttttgaacag taccgacaat gttatagttc
     9541 attgcaatgc gttgtgtaaa atatctaaat ccgacgcctt gaacgcgtcc gaatacttgt
     9601 aaatgtatat gtctcatttt taccacctca taatgttatt atacgtagtt ttacttaaaa
     9661 aaactaataa ttactatagt taatactttg tttgtttcaa gtcgtcaaac ttgattttca
     9721 gaggataaag gtataaaaat aagtatagag ttttgaagta tggaaggggt ctttaataat
     9781 gtggacagtt accaaaatta gagccgatta tgagggatgg tggttattca gtgactggcc
     9841 agaaaacatt gttgaaaaat atcaatatca agattttgat gacatgttta agcactatca
     9901 acaattgatt aatcaatgta aagttcagtt cgataactat gtcacaggca aatataatat
     9961 ttatgcattt tataataatt gtgacatgaa ctattgcgaa gattgtgaag aggatttaca
    10021 aatattttat agtttcattg ttttacaaaa taatgaagta tattataaac taccaataat
    10081 tgattaaatg aatatttttt attattgtat tgcaatttgg taataactgc actataatac
    10141 aagttaagca gatgattatt ccatattgca aagaatattt gagtgaaatt tgcatgtatt
    10201 ttttgtaata tgcgaataag catattgaat gaattttagt cttggaggtt tcagattatg
    10261 aaacaaggta cagttaaatg gtttaacgct gaaaaaggat tcggctttat cgaagttgaa
    10321 ggagaaaatg acgtattcgt acatttttca gcaattaacc aagatggtta caaatcatta
    10381 gaagaaggtc aagctgttga gtttgaagta gttgaaggcg accgcggtcc acaagctgca
    10441 aacgttgtta aactataatt cttagatttg aatcattgat tttaactaac accttaccaa
    10501 gaagtaaggt gtttttatat gcttattaca aatacttaac gcgatgagtt tctcaaccaa
    10561 attgagaact ctttttttat atcaccaaca ttgtttgcaa tagaaaaata tatttatata
    10621 atataattat ggtgtacaac gacgaggtga ttttatgaaa tatcttatcc ttagtttagt
    10681 tgctaattta ttagttttcg gtgtattatc tgcaattggt ttaaatatta atattttagc
    10741 ggcaatgatg attgtattag tgattccgat aatgatttct gggattgtat ttttcaaaac
    10801 taatattgat aaaacataca tattttttaa catcatattc atagactttt attattatat
    10861 ttacaatgta catttaatga ctttgccaaa gtttaataat tatatcaaaa cagagatgat
    10921 ggaattagaa catattgatg tattaataac aagtaaagat tttggttttg atgaaatttt
    10981 attttttaca ctttatttgt tattaatcct tatcgtttta tattatttga aaaagcaatt
    11041 aaagaataaa acctaatttc atgatgcttg tttaaagtgt ggtatgtata tgatgcttta
    11101 aattagttga atagagctaa ctaaattaaa ggattaacga caaaaaataa acactttcta
    11161 actaaaaaag tgctgttctt ccttttttag agtatatgtc caagcatgtt atctacagcc
    11221 atctataatg cttaaaaatt ccccgagtca cagtataatt ttctgttctt cggggattac
    11281 tttatttatt tttattttgt atcattaata ataagttgtc ttaacgattg tcgcttaata
    11341 acttcacgtg ctttgccatc ttttaaaaaa aaacacagaa ggcttttgca tttgattgta
    11401 attggatgcc atagagtaat gatatgcacc agttgataat atagcaagat agtctccacg
    11461 tttgactgat gaaggtaatt tagcgtcttt aataatgata tcaccagact cacataattt
    11521 tccagctata gttacactgt catctgcttc ttcatttcta ttaacaagca atgcttgata
    11581 ctttgcgtca taaagtgcag ttctgatatg atcactcata ccgccatcga ttgaaacata
    11641 tttatttatc tctggaattt ctttaatggt tccaacttca tataaagtaa cgccagcttc
    11701 acctacaata gatcgtcctg gttcaatacc tatttctggt gcatctatac ctagaacttt
    11761 aatttcggat tttattgcgt ctgtaatatc tttaatacca ctttcgatag ggaaactttc
    11821 atcaccttca acatatttaa taccaaagcc accaccaagg tttaataatt caacttgaat
    11881 gtcttgctct ttaagccaac gtaaaacaat tttagcagtt tcaataaatg cttctgtacc
    11941 ttcaatctgt gaaccaatat gacaatgtac accttttaat ttaagatact tagattgttg
    12001 cactttgtta attgctttta tagctaagcc atattgaatt gataatccaa acttactatc
    12061 ttcttgacca gtttgaataa attcgtgtgt atgtgcttca acacctggat taactcgtaa
    12121 tacaacttga accgtatcat tagcatagcg gtctattaat tcaatttctt ctaatgaatc
    12181 tataacaaaa taaccgatgt tattttctaa agcataccta atttcatgtt tcgttttatt
    12241 gttaccatgg aaatggatgc gacttggttc aaatcccgct tctaatgccg tatataattc
    12301 accttcagaa acaacatcta actgtaaatc ttcctcagcc accagtttga ccatttgaag
    12361 acaagtaaat gctttagagg cgtatgaaat attgtatttt aatccactat ctttaaatgc
    12421 gcgatggtaa cggcgcatct gttctctaat ttgtagttca tcataaacaa tggtaggtgt
    12481 accaaagctt tgcgcaatcg tttttaaact aataccatcc attgttaatt cgccattttg
    12541 attatattta acagtcattt gaaatgtact ccttttttaa agaatcatga ttcatagcac
    12601 ttaattgttc agaatttgca ccaacacctt taaaggtata ttcatcgatg cggatatcat
    12661 tattataaag aataacttca tcttgtgcat gtacattgcc atctacttca acaaacatat
    12721 ggctcatcat taatgcacgt atcgggtagc gtttaccatt gataagtgct tcatgtttag
    12781 cacgagttcg taaaattcca tcgccataac cgatatcaac tacagctaat tttgtattgt
    12841 ttttagtcac ttcaaaggca aagctataac cgcaataatc accagcttgt acttcgcgca
    12901 cttgaataac atgtgctttt acagtcaatg actgaactat ttcatgttga ttcagtgaac
    12961 tatatggtct tgaaccgtat aacgcaatac ctacacgcgc atgtgtatgg tggggtagta
    13021 atatttgtcc ttcccgataa aaactcgcac tattttgagc atggattagg tcgaactgat
    13081 aaccttcaga taaaagtgct tcaacaattt ccatccattg tgaacgttca acattataat
    13141 ctgacacatc gaattcatca gcatatccaa aatgggtcca taaaccacta ataatcattt
    13201 ttgcattttg attatgatgg tgatctttca atacttcttt aatttcgttt aaatctttaa
    13261 atccagaccg atgtaataaa ttttcaaatt ctaagtgaac atgaatacct gctaaatcat
    13321 ttttatggtt atagtaatat gtcaacgacg gcaaagtcat gtgtatttga tgttcacgga
    13381 ctaaatcaaa ctcgtaaact gcattcatta aaaagattgt tgcatctgga gcaagttgtc
    13441 taatttgaat tgcttctcgt agtgatgttg tgctaaatgt atctatacct gcatggataa
    13501 actgagttac agcaaattct aggtcatagt gatatgcatt atttttaaca actgccatta
    13561 atggctgatt gtttttgact gtgattgcat tttgtaaaaa tattttctta tttacagacc
    13621 atgttgctgt caatgtatta cacctctttg taattattta ataaattttc gtaaaaatta
    13681 accacgttta ttaacacttt ttcatcaaaa tttaaatatg atgtgtgcaa accagttaca
    13741 aaacctttat cttcatttcg tgttcctata aaaacaaagt aagctggagc tagttgttga
    13801 ccataaaaac taaaatcttc cccaaataag aatggcgttg gtttgtcata gacatttaaa
    13861 tcagctttta ttaaggcgtc ctctatttga gtacgtaatt tcggactatt gattgtaggg
    13921 ggataacctt ctgcaaattt aacttcacag tctacattaa acagaagctt gacactttct
    13981 gctatcttgt gcatttgatt tttaacgatt gttaaatcat caatatcata tgtacgaatt
    14041 gtaccttcta aataaccatt acttggtaca gtgttaatcg cttcaccagc tttaaaatga
    14101 ccaatatgaa caatatttcg ttttaaaccg ttaaggtgaa attgttgaat ttgtgatatt
    14161 tgagttaata catgttgtaa tgcttcacca caagaatgac cttgttcttt atcagcaaca
    14221 tgacttgaca ggcctgttaa gaaaaagcga tactctgttg cgctggccgt aatttcttca
    14281 tctcttatca ctgcaatgcc ttcatcagca aatgggttaa catgaatacc aaataccgct
    14341 tcaattggat acttatcaaa ggcaccggct tttattaatc gatttgcacc gccaccagtt
    14401 tcttctgcag gttggaaaat gaaaacgaca ttttgcggta attgccccgc atcttgcatg
    14461 tctttgcaac gttgtacaaa aagcattaat gtagttgtat gaccatcatg tccacaagca
    14521 tgcatcacat gatcagattg actgcgataa ggtacatcat tttcctccaa aataggtaat
    14581 gcatcaatat cagctctata cgctatcgta tgtgagccat taccttctaa gtatgcaatg
    14641 acgccagttt ccaatgggca atcgtattta atatttaaac tatctaaaaa cgctttaata
    14701 taagcagttg tttcaaattc atgtaagctt aattcaggat gttgatgtaa atgacggcga
    14761 tgtttcgtaa caaattctaa ttcattcata attatcaatc ctttgtgtta aattactata
    14821 taaatagtgt aacgtatttc gaaatttgtg atcataagtt tatttaatga taaacaataa
    14881 ggttgagaca taatcgtatc tcaaccttga aattattata cgttgacgtc actagtcatt
    14941 cagttttctt aatgctgcta caatctcttt tttagtatct tgtacttcag aagcttgctt
    15001 aatcactttc gcaggtgtac cagcaacaac tgcaccagct ggtacatctt gagtcacaat
    15061 cgcgccagct gcaacaatag cacctttacc aacacgtaca ccttctaaaa taactgcatt
    15121 tgcgccgatt aatacatcat cctcgattat aactggtgaa gcactagggg gttcaatcac
    15181 acctgctaat actgcgccag cccctacatg tacattttta ccagttgtag cacgaccacc
    15241 gagagtagca ttcatatcaa tcatcgtacc ttcgccaacg actgcgccaa tattaattgt
    15301 tgcacccatc ataacgacag caccatcttc aataatagct tgttctctaa taaatgcacc
    15361 tggttcaata cgtgcattag tatttgttaa gtcttttaat ggaatggcag aattgcgacg
    15421 atccatttca atttctatat ctccgaattg actaccatat gcttcgtaaa aaggtttcca
    15481 atcatctgct tcacaaaaga ttactttaga ttgttctgaa ccaaatactt taaaactttc
    15541 tggatatgtg atgccttcaa aattgccatt taaatatact ttaattggtg tagatttttt
    15601 agcatcactt atatattgaa taatttcttc agctgttaaa tgttgtacca taaaataatc
    15661 gatctccttt aatatgttta taggttgtca aacgtataaa agccgtttgg tttattaact
    15721 aagcgttctg ctgcttgtat tgcaccattc gcaaaaatat cttttgattg tgcacgatgc
    15781 gtgatttgaa tcgtttcatc agtgccggca aatagaactt catgttcacc gacaatcgta
    15841 cctccacgaa tagaatgaat accaatatct tgtggctggc gtttttcatt taattcatgt
    15901 ctatcataaa caggagttac attttctttc aaagatacaa tcacgtcata taatttttct
    15961 aacgtaccgc ttggtgcatc tactttttta ttatgatgtg cctcagtcaa ttcgatgtcg
    16021 aaatcatcaa gtaggggaac agcagctgct aaaattttag tcagtgcatg aacgccataa
    16081 ctcatgttcg cgctgaaaaa cacaggcata tttttactca attcatctaa cttattaagt
    16141 agtttttctt tctcgccagt tgttgccacg actaatggca aatgaaacgc ttcatctaat
    16201 aaagggaaaa gcagatttgg atttgaaaaa tctattgcaa catctgcatc tttaacatca
    16261 gcaatatgtt ggtattgttg atatggcgtt gttgctttcg gtgtattttc aatgacccca
    16321 acgatttcat gtcctttttc ttctgctaat ctagcaacgc gctgattcat tgcaccatag
    16381 ccaattagta atattttcac tcattttcac ccgctttaaa tgtgtcatat gcttcacgaa
    16441 gcactttagt atctgtatct tctaggctaa ccaatggtaa acgtaattca tagtttccaa
    16501 atcctaaata acttgttaaa gctttaatag gaattgggtt aatatcaact gatagggctg
    16561 ataacagtgt gccgattggt ttaaattgat cttgaatatc taatccactt tgttgagcat
    16621 cgtataacgc ttgaaattct ttaggaatga cattggcaat aacagagata accccttgac
    16681 cgccacgttg atagtattcg acgacgttgt catcattgcc actatataat gcaaatgaat
    16741 ttgtatcaat tcgctttttc acttcttcta aatactcaaa atcattcgta gcatctttta
    16801 aagcaactat ataaggatgt tgacttaata tttctacagt ttctggttca attgtcatgt
    16861 tcgttcttga aggaacattg tacagcacga ctggtaattt cacagcatct gcaatcgctt
    16921 caaaatgttt tactaaacca cgttgattcg ttttgttgta gtagggtgta attaacataa
    16981 ttgcatcagc ccctaaggct ttagcttgga ttgaagcttg gattgacttt tcagtatcat
    17041 tagttccagt tccagctatg acaggaacac gtttatctac aagatcaata actgttttta
    17101 gaatgagttc tttttcatct gttgttaaag tagggctctc agcagtagtt ccattaacga
    17161 tgattgcttg ggcattattt tctagtaaaa aattaacgtg tgctttcaaa gcttcaagat
    17221 taactttgtt atttgtaaaa ggggttgtaa gtgcaacgcc aacaccctca aataaatgtg
    17281 tcattttaat tcgctccttt taaacgcata acttgttcca atacttgtac agcatttaat
    17341 gcagcacctt ttaataaatt gtctgatgta caccatacat ggaaagtatt ttctaatgaa
    17401 tcatctctac gtatacggcc aacaaatact tcatctttat tagtagaatt gattgccatt
    17461 ggatattcat tgttctctgg attgtctact aaaacaacgc gatcatcttg atcaaataat
    17521 gctttaatat cttctgcagt cgcttctttt tcaagtgtta catcaatttc aacactatga
    17581 ctatcttgaa caggaacacg tgcacatgtt gctgttactt ttaagtctgg cgcatttaaa
    17641 atttttctcg tctcatcaat cattttttgt tcttcttttg tatatccgtt ttctaaaaac
    17701 acatcaatat gcggtaacac attattataa attggatgtg gatatgcttc tggtgcttta
    17761 ccatttacac cttcagctaa atctttctta cctttcatcc ctgaacctga tacagcttga
    17821 tatgttgtat atgccactcg ttttaaacca taagcatctt gcaatacttt taatggcaca
    17881 acagactgaa tcgtagagca gtttggattg gcaatgatac ctcttgtaaa agtaggttca
    17941 ttgacttccg gaacgattaa atcaatatct tctgccatac gccattgact tgaattgtct
    18001 ataacgattg caccaccttt ttcaaagagt ggggcaaagt gttcgcttgt accgccacca
    18061 gcactcatta atacataatc gaaatgttca cttgcacgag catcagttaa ttcttgaact
    18121 gtatatgttt ttccttgaaa ttcaacttct ttccctgcag aacgtgctga tgaaaataat
    18181 actaattcat cgaaaggaat atttttacga tttaatgtct ccaacatttt tgtacctact
    18241 aatcctgttg cacccacaac tgctaatttt gtcataactt gtcactccat tttataataa
    18301 tttccaattt ttagaatatt ttaacaatca ttttaccatt aaatgttaaa tgcgtcatat
    18361 agtttttcca ccgcttgttg cccattaaaa tcatcaatga cgtatgaaat acttatttca
    18421 gatgttgttg tttggtagaa aggtatatta ttttcaatta atgtcaaaaa tgcttttgat
    18481 gccacacctg acatatcacg catgcctgag ccaattaatg aaattttgac ataatgctca
    18541 ttgattttat aagctaatgc ttcatattga ttctttaatg ttttaagaat catagaaatt
    18601 tgatgaaaat cactatcttt aatcgtgaag gatagttgta gtccatccaa gttgacgatt
    18661 tgtgaaatca tatcaacatt tacagcacct tcttcaagtt ccgtaaatag ttgggtaagt
    18721 agctgattgt caggtagggg ataactaatt gttacatgca tcatatgttt atccaaagcc
    18781 acaccagtaa ctgcttttct ctctaatatt tcttcatttg acataatcca tgttcctttc
    18841 acgttcgata aagtttttcc taaatataaa gggatattat agtttttagc taattcaaca
    18901 cttcttgttt caagtacacc agcacctaaa gcgctcattt ccatcatttc ttcatatgag
    18961 acgatgtcta gtcgtttagc ttttggtaaa agtcttgggt cagtggcata cacaccatca
    19021 acgtcggtat aaatttcaca aggtatttga ttactaacag caagtgccac agcggtcgta
    19081 tcagaaccac ctctgcctaa agttgttaat tcctgatggt cattgatgcc ttgaaatcca
    19141 gcaactacta aaatatcgtt ttcttgaaag gcttgttcaa atgtttgagg attaatttga
    19201 gcaattttac tttttaaatg atggccaatg gttttaatac ccgcttgata gccagtcatt
    19261 gcttttgcat tcacaccgat atcatttaat accattgata aataagatac agtttgttgc
    19321 tctccggttg tcaataataa tgccagttct tgttgttttg gtgttttagt caatgtcgat
    19381 acattcgtca ttaattgatc tgttgtgtta cccatagcac ttacaacgac aattaattgt
    19441 tcatcttgat tgacccgctc ctttaacatt tcagcgatcc tttttatttt tgtaaaatca
    19501 ctgacggatg atccgccaaa tttcaacacg cttcttgtta ccatataatc ctcctaattg
    19561 ttaggaaggg cacacaataa taaaaaaaga acaagttcgg aatgcgaact tgctcttaat
    19621 ctatatacaa gtgatgcact ccattatttt taaataatga caaactctca gctcttaacc
    19681 aaaaagtcca acaaattata actgctatta taatcgcttc
//
LOCUS       DAMZPP010000038        19465 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMZPP010000038 DAMZPP010000000
VERSION     DAMZPP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19465
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.038"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(223..1509)
                     /locus_tag="POH73_001045"
     CDS             complement(223..1509)
                     /locus_tag="POH73_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6887011.1"
                     /translation="MNPLAQSLNEQLQQSNATAFAMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSRPIVTNALTHGLSLVGDLFVNQDDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVEAIKA
                     LANKGTKVIAVVDDAYYGLFYEDVYTQSLFTALTNLHSNAILPIRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEALRKHLIDKYSIG
                     VIALNATDIRIAFSCVEKDDIPHVFDSIAKAIDDLR"
     gene            1709..1807
                     /locus_tag="POH73_001046"
     CDS             1709..1807
                     /locus_tag="POH73_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887012.1"
                     /translation="MMWWQEGMMTLITGGLPIIFRLWLELKWKNKK"
     gene            2049..2225
                     /locus_tag="POH73_001047"
     CDS             2049..2225
                     /locus_tag="POH73_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887013.1"
                     /translation="MKHLTKIFVALAIILFIVGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNNK"
     gene            2483..2863
                     /locus_tag="POH73_001048"
     CDS             2483..2863
                     /locus_tag="POH73_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6887014.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            2860..3756
                     /gene="pmtA"
                     /locus_tag="POH73_001049"
     CDS             2860..3756
                     /gene="pmtA"
                     /locus_tag="POH73_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HDI6887015.1"
                     /translation="MNAIEVSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYAENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIFEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            3757..4437
                     /gene="pmtB"
                     /locus_tag="POH73_001050"
     CDS             3757..4437
                     /gene="pmtB"
                     /locus_tag="POH73_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HDI6887016.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLVGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            4434..5306
                     /gene="pmtC"
                     /locus_tag="POH73_001051"
     CDS             4434..5306
                     /gene="pmtC"
                     /locus_tag="POH73_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HDI6887017.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            5306..6046
                     /gene="pmtD"
                     /locus_tag="POH73_001052"
     CDS             5306..6046
                     /gene="pmtD"
                     /locus_tag="POH73_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HDI6887018.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGDKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(6110..6673)
                     /locus_tag="POH73_001053"
     CDS             complement(6110..6673)
                     /locus_tag="POH73_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI6887019.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            6794..7318
                     /locus_tag="POH73_001054"
     CDS             6794..7318
                     /locus_tag="POH73_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887020.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            7378..7935
                     /locus_tag="POH73_001055"
     CDS             7378..7935
                     /locus_tag="POH73_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDI6887021.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEE
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            7932..8774
                     /locus_tag="POH73_001056"
     CDS             7932..8774
                     /locus_tag="POH73_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDI6887022.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNISSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSPTYISKSNIKNSNIKVVIGMLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFINGKVGKSDSVLEFYTIDGN
                     IKVE"
     gene            complement(8831..9871)
                     /locus_tag="POH73_001057"
     CDS             complement(8831..9871)
                     /locus_tag="POH73_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDI6887023.1"
                     /translation="MQQVTSDIMTFIGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYHPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            10322..10495
                     /locus_tag="POH73_001058"
     CDS             10322..10495
                     /locus_tag="POH73_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887024.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
     gene            complement(10552..10953)
                     /locus_tag="POH73_001059"
     CDS             complement(10552..10953)
                     /locus_tag="POH73_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HDI6887025.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            11224..12252
                     /locus_tag="POH73_001060"
     CDS             11224..12252
                     /locus_tag="POH73_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDI6887026.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            complement(12372..13751)
                     /locus_tag="POH73_001061"
     CDS             complement(12372..13751)
                     /locus_tag="POH73_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDI6887027.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKNARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTNDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            13803..13976
                     /locus_tag="POH73_001062"
     CDS             13803..13976
                     /locus_tag="POH73_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887028.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            complement(14167..15096)
                     /locus_tag="POH73_001063"
     CDS             complement(14167..15096)
                     /locus_tag="POH73_001063"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDI6887029.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(15149..15709)
                     /locus_tag="POH73_001064"
     CDS             complement(15149..15709)
                     /locus_tag="POH73_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDI6887030.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            16082..17179
                     /locus_tag="POH73_001065"
     CDS             16082..17179
                     /locus_tag="POH73_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HDI6887031.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNHGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGHRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKNCSFEGFVDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENINIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            17384..18946
                     /locus_tag="POH73_001066"
     CDS             17384..18946
                     /locus_tag="POH73_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDI6887032.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSTGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGHEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYIMPIV
                     LGIDINQPLPLK"
     gene            complement(18957..19064)
                     /locus_tag="POH73_001067"
     CDS             complement(18957..19064)
                     /locus_tag="POH73_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887033.1"
                     /translation="MVDFLLFVLFLHDATVYVSLPLVQAIANVTFYFSF"
     gene            complement(19136..>19465)
                     /locus_tag="POH73_001068"
     CDS             complement(19136..>19465)
                     /locus_tag="POH73_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDI6887034.1"
                     /translation="IDTHIEDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGL
                     LASVLNTFALFNINLSWIESRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKV
                     EMIGAFN"
BASE COUNT         6635 a         2711 c         3359 g         6760 t
ORIGIN      
        1 atttgtagag atgtgatact tgaagtgatt tgccacacgt ttgataaatt tatctaaggt
       61 gtatcaagtt ttgttaggag agatgtataa aactaattag gtatagcgat taataagttg
      121 ctgaataaaa tatatcttat gatgctttga aagaaggaat aattttaaaa ataaaaaacc
      181 aataatccga gtcatacaca tcggattatt ggttgaagtt aattatctta agtcatcaat
      241 tgctttagca attgaatcaa atacatgtgg gatatcgtct ttttctacac aactaaacgc
      301 aatacgaata tctgtagcat taagtgcaat gacaccaata gaatacttgt caattaaatg
      361 cttgcgcaat gcttcagggt caacatcatg aacttttatt gccataaagt atccagagtt
      421 aaagtcatat gcttgccaat gtgaatgata ttgatcagca tagacaactt ccttagtgac
      481 ttcatagcgt tcttttaatg tttgaatatt ttgttcgatt tctttatcga attgtttatt
      541 attttttaat acatgcttaa cagcgctttg tgttggaagc ggtccactag aaatgttgct
      601 tcgtataaga ccttttactt tggcttctaa aacttcttta gttgtttgat cagacgttcc
      661 aaatgtcata aatccaacac ggaatcccca tgcgaaaaat tcttttgttg ccccatctaa
      721 acgaataggt aatattgcat ttgaatgtaa attagttaat gcagtaaata atgattgagt
      781 atacacatct tcatagaata aaccatagta tgcatcatcc acaacagcta taacttttgt
      841 acctttatta gctaatgctt taattgcttc gacgatcgta gtcacttctt tatgcgtagg
      901 tgtgtaacct gtcggattat taggataatt taaaatcata atgactttat ctttattgta
      961 tgattgtaaa gcttctacta gtgaatcagt agtataatgc ccgtctttat caaagatagg
     1021 atatgtttga aggtttgcac catttcttgt attgaaaaca agtttgtaat taccccaatt
     1081 atgctctggt aacaagatag tgtcatcttg atttacaaat aaatcgccaa ctagagataa
     1141 accatgtgtt aatgcattcg taacaattgg tcgtgacatg ttgtcgattg atagctctgg
     1201 attgtcacgc aacatttttt gttgccataa atcacgtaat tcttcgatgc cttgtggtgg
     1261 cgcataaggg aatatttcat ctggagttaa atcattaaac attgcatcta aagatgatgc
     1321 aaacattttt ccgtctttat ttgtcgccat acctatagtt gcattatatg ttgtactctt
     1381 tgcttcagca gattgagata aaatgccctt tggataaaac atattttgac ctaagtcaga
     1441 taacattgca aaggcagttg catttgactg ttgaagttgt tcatttaagc tttgggctaa
     1501 aggattcata attgcttaac ctctctttaa aaaaattgta tgcctatata ttatcttcaa
     1561 ttataccaaa gataaagtgt tgttggtgaa ttaacgaata atatgtaaaa tccgctacca
     1621 aaatacgaac acatgttctg tttttgtgtt agaataggtg taggtcatcc ttcagtatga
     1681 tactttagtc gaacggaggt gtagtgtaat gatgtggtgg caagaaggga tgatgacact
     1741 tattacaggt ggcttaccaa ttatttttcg gctttggtta gaactgaaat ggaaaaataa
     1801 aaaatgatcc tacacacata aataacccca agtctgctgg taccagactt ggggttgtgt
     1861 agtataattc tgtggataca cttaaataat atcattgaac acaatatatc gcaatcaata
     1921 atcattttga atgcatagtt ttgttgtcat gtaagccaat gagagattgg ttatattaca
     1981 ctatacaata tattaagtta tgataaatct ttatatttgt tataatgtcg atgttgaggt
     2041 gaaataaatt gaaacatcta acaaaaatat ttgtagcatt agcaattatt ttatttattg
     2101 ttggttatta tttacaagca acaaaccatg agagccaagg tataaaacta ttattggcag
     2161 caattatgtt tatgatttgt gcttttataa gtagaagtaa tgatcgacgt aaaaataaca
     2221 aatagaattg actgttttaa acgactgttc ttaatgacgc gacaatcacc gtcaaagtat
     2281 aagaatattc gttttgacag tcttttttat tgaagtagac aatgcaagat taaatggtag
     2341 agtcatttca ttttattcaa ctgctagtaa tacaataccc agtgttattt ctgaagactg
     2401 gttgataatg aaacatgcat atttacaaaa ctgtgtatat tgtgtatatt gtatatatac
     2461 agaaggtgat agggggacgt tgatgaaaat aattttaaaa aacaatagtg attttccgat
     2521 ttatgaacag attaagcaac aagtaaaaca aaatatttta aagggacatg ttgctcctgg
     2581 agagcatttg ccatcaatga gggaacttgc caaagatctt caagtaagtt taattactac
     2641 caaacgtgct tatgaagatt tagagaaaga tgggtttgtt accacaatta gaggaaaagg
     2701 gacctttgtt aaggagcaag atagttctat tttaaaagag aaacaatttt ttaccattga
     2761 aaatttggtt aaagaattgg ttaatgaagc gcaagccatc gaaatgtcac ttgaggaact
     2821 tcaagatatt ttaacgttca tttatgagga ggaatcatca tgaatgccat agaagtaagt
     2881 aatgttaatt attcaagtga tcagtttaat ttaaagaata tctctttcaa agttcctcaa
     2941 ggattcgtca ctggttttat tggtagaaat ggcgctggta aaaccacaat aataaggtta
     3001 attatggatt tgtatcaacc acaaactggt gttattagag tattagaaga ggatatggct
     3061 ctcaatccga tagagttaaa gaatagaatt gggtttgttt acgctgaaaa ttattttaat
     3121 gagagatgga ctacgaagca acttgaaaaa atgattgccc ctttttatcg taaatgggac
     3181 catcaagtat ttgaatttta tcttgaaaag ttcgatttac caatcaataa atcaataaaa
     3241 acattttcga ctggcatgaa aatgaaatta tcattagcag ttgcattttc acatcatgct
     3301 gaattgtata tttttgatga gcctacttca ggtcttgatc ccttagctag aaatgaacta
     3361 ttggagataa ttcagcaaga attaattgat gaaaataaaa caatattcat gtcgacacac
     3421 attatctcag atttagaaaa aatagctgat tatatcattc acttaagtga cggtgaagtt
     3481 atcttaaatg gttcaaaaga acaactgtta caaagatatc aagttgtgag tggtgcaatt
     3541 gaggaccttg atgatgaatt agcatctcta cttatttttg aagaacataa gcgtacagga
     3601 tttataggat taacagaaca tgcgcaagta tttaaagaaa ttttaggtca taaagtgaat
     3661 atcactacac cttctattga aaacttaatg gtttatcttg aaaagagaaa acctaaatat
     3721 catgaaaaca tcaaattgat ggaagaaggt ttttaaatga aggcattgtt aattaggaat
     3781 tttaaattac gacgatatac acttatcatc tatgttctat tattaacctt atatcctttt
     3841 tatataatgc ttgactcaac aaagttcttt tacctattgc aatcttttat aagtccaaca
     3901 atattgatta tttggatttt agatgctggt cacttattta gactcaatcg tagattaggt
     3961 ggtaatgatt catattattt ttatatgagt ttacctgtgt caaaaaaaca attgcttaat
     4021 gcgaactata ttacatgcat tgttttaaca ttagttggaa cgcttgttat tagtttatat
     4081 gcttatgaag cagatgtgat tgaaccaaat tcaatttatt tttcaactgc atatgcattt
     4141 gtcatatcta acttcttgtc tataccaatt gcatttagtc aatttacaga attgcgcaga
     4201 gtcaaagtgc cctatggtat atacgtgttt actattatca ttttagttcc atttttattt
     4261 tcaattgcaa tagtgttggt gaattatttt gttctaagtc aatcatcatt cccagatcta
     4321 tattcatata ttttaaatat tggttttcta attataagca ttgttatact tattgttaat
     4381 tattttaaac agcttaataa aataaatact agaaaattta aaggaggcag tcgatgaaat
     4441 tagaacatat tacaaaaaaa tacggctcaa atgtcgtttt aaatgatatt gattttgact
     4501 ttggcgatag tagaattgtt ggattaatag ggaaaaacgg tgttggtaaa acaaccgtta
     4561 tgaaagtaat gaatggtaat attattaaat ttgatggaaa agtagatatt gataatgcag
     4621 ataatatcgg ttttttaatt gagcatccta aattatatga taataaatca ggattgtata
     4681 acttgaaatt atttgcacaa gtattaggta agggttttga taaagcatac acagacaaaa
     4741 ttatagatgc atttggtatg agaccttata ttaaaaagaa agttaagaaa tattcaatgg
     4801 ggatgaagca aaagttagca attgcagtat ctttaatgaa taaacctaaa tttttaatct
     4861 tggatgagcc tacaaatggt atggatccag atggctcaat tgatgtgctg actacaatta
     4921 agtctctagt aaatgaactt gatatgagaa ttctaatatc aagtcataag ttagaagata
     4981 ttgaattaat ttgtgataga gctgtatttt tgagagacgg acattttgtt caagatgtaa
     5041 acatggagga aggtgttgca tctgacacaa cgatagttac tgttgatcat aaagactttg
     5101 atagaactga aaaatatctt gcagagcatt tccaattaca aaatgtcgac aaagcagatg
     5161 gacatttaat gatcaatgca caaaaaaatt atcaagttat actaaaagca ttatctgaat
     5221 tagatattta tccgaaatat attgaaacac gtaaaagttc attgcgtgat acgtacttca
     5281 atataaatca aagaggtgat aaataatgag aattttaaat ttagttaagt atgattttta
     5341 tagtatattt aaaagtcctt taacatattt agcgatacta gtcgtatcta gtttgattgc
     5401 aacgcaaagt atacttatgg caaattcgat ggataacccg aaacatatta ttgtctatgg
     5461 atctgtattt gctgcagcaa aatggttatt gttaataatt ggattaatgt ttgttgttaa
     5521 gacaattacg cgtgattttt cacaaggtac aatccaacta tatatgagta aagttaaaac
     5581 acgtgttgga tacattattt cgaaaacaat ttcaattatt ttaatttcaa tattatttgc
     5641 attaattcat tatgtgattt tgattgttgt gcaggcatct agtaatggga aaaatttggc
     5701 gttttctaaa tatgtagata atttatggtt cttcctaatc tttttactat tctttggctt
     5761 gtttttattc ttaatcacac ttgcatcaca aaaaacagca atgatatttt cattaggtgt
     5821 atttttagta cttattgtac cgtttattaa accattcatt acatttattc caagatatgg
     5881 tgataaagtt ttagatgctt ttgattatat cccttttgct tacttaactg ataaaatgat
     5941 tagctctaac tttgatttta gcaattggca atgggtaatt tcattaggtt ctatagtgat
     6001 attcttcatt ttgaatatct tatatgtcgc taaaaaagac atttaataaa aaattttgag
     6061 gttgggaatt ttaaattttc ccaacctcaa agtttgtctt attgtaaatt tatttatttt
     6121 ctaatttgtt taggatggaa ttataaactg ctttccaaaa tgaagcgtca gttttatagc
     6181 gatttgatat aactaagtgt gtttcttttt ctaaatctgc atagtctgga tgatctttgc
     6241 tcggtaattt atcggcacga acatcagtta caaatttttg gacttcattt gctcttggtc
     6301 cccaaactgt ttcttgttcg aattgatcat tcaagaatac gaagatagga attgcacgtg
     6361 atttaccatt tgttaaatat tgatcgatca gttttgtatc atcatctcta tggaacacgc
     6421 gcacttctaa atttaatgct tcactgatgt gttttagaat tgggagattc atcattgcat
     6481 ctccacacca gtcttcagta attactaata ctttagaata attcatctct tttatttttt
     6541 tgatgcgtga atcatcttct ggtaactcaa atgattgata gatactgaga acggtatctt
     6601 gatttgtctt cattccatca atgtattcat ttaagggttg gctatttttg aaataagttt
     6661 ctaaatttgt cattgtaaaa acctccttta gcatttacaa cattatacca atttatagta
     6721 ataaaaggta atgaaataaa ttaattgcaa attctttgtt aatttttgtt aaggatgaaa
     6781 cgggaagcac cttatgctat atttaaataa gtacaaagaa agggtgacat cagtgcgtat
     6841 tcaaaatcgc tgggttgtgt ttatattatt tttaatctgt tcttttggtg tattaattgg
     6901 tttatatcaa tatcgtcata cgaaaactgt ggatttgtct aatcttgaaa taaacgatat
     6961 taaattaaat gagaagtttg ataaaaaagg ctatgaagta aataaaaaaa ttaagtttga
     7021 tcgttttaaa ttttataaca gtaaagctca ccctgacctt accgttaaag tgagagaaaa
     7081 ggataacatc gttaagggga taatattagt aagagatgaa aagatacata ctaattttga
     7141 tggaggaatt ggttcgccga taaataacgc gattgaaaat cttggattcg gatataaaag
     7201 aacaaaagtt ggcaatgatt tctcatcggt aaagtatatt gatagagata accatttaaa
     7261 attaaactta ctttatcaag atttagaaat taaacgtatt gaatttttta gtaaatagct
     7321 ttaggtctta aagttataaa aaacgaatga ataattttat tgggatgagt gacctatatg
     7381 aacaagatta cttatttaaa tgaattagaa acagcacttg atgcgctacc taggcattta
     7441 agagatcaaa aaatgtatga atatgaacgt tatttttatg agcaagaact taatggcgtt
     7501 gatgaagagg aaattttaaa gaagttaaaa gacccacaag atgttgcagc tgaaacaaaa
     7561 gctagaagtg ttattgatta tgctgaatct aaaccaactt ttgaaaatat ttcaagagct
     7621 gttgctgctt cattaagttt aggcattcta tctatttttg tcatccttat accagtatct
     7681 atagttggat tatttgtatt agcattattt ttaatatcac ttttgctgct gttttgtcca
     7741 attattttat tagcatcagc aatatccaga ggaattgtag actcaattag taatgtattt
     7801 tttgccatat catattcagg attaggatta gtatttatca ttgtcatatt taagatttta
     7861 gaatacattt atcgtttaat cttaaaatat ttactttggt atattaaaac tgtcaaagga
     7921 agcgttagaa aatgaagaaa ttctttttta ttgggctttt agtgtttgtt gtctttttta
     7981 cagcagcaac cattatttgg ttcagctatg ataaaaacaa atatggtact aaacaatatg
     8041 ataaaacatt caaagacgat gcttttgaca atgtatctat aaatttggat agtacagaac
     8101 ttcgtataaa gcgggggaat caatttagag ttaaatatga tggtgacaat gatatattaa
     8161 ttaatatagt agataagacg ttgaagatta gtgataaaag gtctaagaca agaggatatg
     8221 caattgatat gaatcctttt catgagaata agaaaacgtt aacgattgaa atgcctgata
     8281 aaatgattaa acgtttaaat atatcgtctg gagcaggaag tgttagaatc agtgatgttg
     8341 atttagagaa cacaagtatt caaagcatta acggtgaagt agttattaaa aactcaaatc
     8401 ttgacgcact agattcaaag acaaataata gtcctactta tattagtaaa agtaacatta
     8461 aaaatagtaa tattaaagtt gttattggta tgctacaaat cgacaagagt caaattaaac
     8521 aatccatatt tttaaacgat catggtgaca ttgaatttaa aaacatgcca tcaaaagtag
     8581 atgcaaaagc ttctactaaa caaggagata ttcgttttaa gtatgatagt aaacctgaag
     8641 atactatact gaagctaaat ccgggaacgg gtgatagcgt agttaaaaat aaaacgttta
     8701 ttaatggtaa agttgggaaa agcgatagtg ttttagaatt ttatacgatt gatggtaata
     8761 tcaaagttga ataaataaag gatgtaagca ccgatattag gaagcataat ttctctaata
     8821 tcggtgttat ttatttgttg gcaaaagtta agtcggtatc tatattgcca gtaaagtgag
     8881 tgatattaag gtcttgacca tctaaccatg atttgaaatc tattatttct ggtggcgcat
     8941 tttctcccaa tgtaaaatat gcagttaatg tttcagggtg atacattgat gtatggatgg
     9001 tgccagacca gcttttgaat agtttactgt aaatttcata ctgaggattg ttgaataact
     9061 taaatgctgt agtcatatct aaattatcat tagtttgtga aatggtacgc gctagtcttt
     9121 ctttagattc ttttgtataa ttgcgatttt catgtgttaa tatttcaaaa tgatttgtac
     9181 atatattatc ataacgaaca tctattgatc tcggcgtcac ttcaacaatt gcatggttca
     9241 atgatttgtc catcagtatg tagctaaatg agcttctgtg tggtatttct ttcaataatt
     9301 ggattgcttc tgttacattt cggcaatttt caagaattag acgaccaatc atataacata
     9361 caaaaccatt tgctggtttc ttccggtgca taaagttata gcccatagtt aatcctgact
     9421 cattcatacc atccattctt ccagttaccc ttgatacagg accaatttga gctaaaccgc
     9481 tatctgtagg ttgatatagt aagtagcgtc catcataagt tgcagggtgg taatcgtaat
     9541 ttctaaccat gaagtctttg ccttgaaaga cagtgcaacc actttctttt aaatcggtaa
     9601 aacgataatg tccaaagttt aaaataattt gacgtgttgg cattttgagt atactttgta
     9661 gtcccattaa ttcttcccat atttgaggtg cgtaagtttg gaatatttga taagtttcat
     9721 ttacatctat atcgaaacgt gggacacgtt ttttccattc tttttctcta ttttttagaa
     9781 gaggtgtttg ttgaagccat ttaccagttt taacacctaa ctcgaaatgt gaacctataa
     9841 aagtcataat atctgatgtc acttgttgca tatcatcggc ccctttcttt ttagttgtaa
     9901 tatattgtaa ataaatagta atcgtatgta tattgaatgt catgttaaat aaagttatat
     9961 tttactaaat gaaatataaa attgtttgag gtgatttctc ggtgtataag acttatcaat
    10021 ctgttaaaac atatttttat agatggtggg gatattaagt taaaaactta aaatcatctt
    10081 atcataaata acaatcttaa gttagcattc acgataatag tcattgttaa cattagcata
    10141 taaggtcatg tcacgttgaa acagaggttc ctcggcattt ttgaaaataa tcattagctt
    10201 gctcgctata taatttgatg aatatatttc gtaaaagtgg gtatttattt aatgattatt
    10261 ctatatatga tagtataatg aaatgtagat aggtatttaa tttaacagag gtgaaattga
    10321 gatgtggaat tttattaaat gtgtgtttaa attcgtattt agcttagttg ctattacaac
    10381 attagttgct ggtgttggtg tagtagcatt tgcttatatc tttaaaaaag attttgaaga
    10441 tattgaaaga aaaactaaag aaattatttc tgatatcgaa agtaaaaata actaataaca
    10501 tttaggcaag gacaatattg attgttcttg cctttaatta tgttcaaagc attaaggtaa
    10561 caagacaata tctaagatat caaagataga aattttttga cgttgttgct gattgtaaac
    10621 ataaccatca atttcataat taatagcatc aatacgataa atggttaagc gtactgaatc
    10681 tacaaagcca ttattataaa atttaacttc tacaggttgg gcatattgta gcgcctcgtg
    10741 tagccgaatg tttagctcag ccaattgatc atctgataat acaggacgtg taattttgtt
    10801 ttggtcgata atgtattgtt gaatcgtttc gaattgttcg ggtaatgttg caaaaggagc
    10861 ccatttaatc atgcctcttc ccataggtat attgttatct agtaattctc ttggaacgtt
    10921 acgataatca gtttcttctt cataacttgt catccttaat tcaccccaat ctgataatta
    10981 cattatacga acatgtgttc tattttgcaa caaaaatttt gtggaagcat aaacgcgtta
    11041 ataattaatg ctcgggtaag taaaaaagag ggattaatta aaatcgaata atgacacatc
    11101 accagcaaat agttctttta aagtagttaa acagttttag ctttaaggaa atgataaatg
    11161 attgttaatt ctagctaaaa tttaataaaa tgaaaataag actaacatgg aggggtaaaa
    11221 gtaatgacaa atggatatat tggttcttac actaaaaaga atggtaaagg gatttatcgt
    11281 tttgaattaa acgaaaatca gtcacgtatt gatttattag aaacaggatt tgaattagaa
    11341 gcgtctacat atttggtgcg taataacgaa gttttatatg gaatcaacaa agaaggagaa
    11401 caatgcggtg ttgcgagttt gaaaattgat gacaatggtg aattgcattt attaaataaa
    11461 tgtttgtctt caaaagctgg tacaggttgt tatgtatcga tttcagaaga taaacgatat
    11521 ttatttgaag cggtatatgg agctggcatc atacgtatgt atgaattaaa cacgcacaca
    11581 ggtgaaatta tacgtctaat tcaagaactt gcgcatgatt ttccaacagg tacacatgaa
    11641 agacaagatc atccacacgc acattatatt aatcaaactc cagatggtaa gtacgttgca
    11701 gtaacagatt taggtgctga tcgtatcgtt acttataaat ttgatgacaa cgggtttgaa
    11761 ttttataaag aatctttatt taaagatagt gatgggacaa gacatattga atttcatgat
    11821 aatggaaaat ttgcttatgt cgtacacgaa ttatccaata ctgtgagtgt tgcagaatat
    11881 aatgacggta aatttgaaga gctcgagcgt catttaacaa ttcctgaaaa ctttgatgga
    11941 gatactaaac ttgcagcagt gcgtttgtct catgatcaac aattcttata tgtatctaat
    12001 agagggcatg atagcattgc aatttttaaa gttcttgata atggtcaaca cttagaacta
    12061 gtaacaatta ctgaaagtgg tggtcaattc ccaagagatt ttaatattgc ctcatcagat
    12121 gaccttttag tttgtgctca tgagcaagga gattcagttg taactgtttt cgaaagaaat
    12181 aaagaaacag gtaaaattac gctatgtgat aacactcgtg tagcatctga aggtgtatgt
    12241 gtcatatttt aatctttaat taatcatgat aaaaagaaaa ccatgtttcc aaaaaatttg
    12301 tgtatacctt gaaatttatt gttttcaaga acatcaaatt atggaagcat ggtttatttt
    12361 tgtctcaaaa attaattttt aaagaaagct ttgatgtatt taaatttacc tttatatggt
    12421 ggtaaatgga cacctgattc taatcgtgta gatttgaaaa tgtagctttt ttcatgtgta
    12481 aaagtgtcaa atgaatattt accatgatag cgtcccatac ctgaggcgcc aacaccacca
    12541 aatggtaatt taggattcgc taggtgcatc aatgtatcat taatagcgcc gccgccaaat
    12601 gataactcgt ttattacacg ctgcgtagca ttttcatctt cgctaaataa atataaactc
    12661 aaaggttttg gtctttggtg aataaaggct attgcttcat ccaatgactg atacgttaaa
    12721 atcggtaaga taggaccaaa aatttcttct tgcataattg ctgaatcatt tgtaacgtga
    12781 tctaacaatg ttggttctat ataacgttca tcctcatcac tatgaccacc aaatacaata
    12841 ttcatttgtg cactgttaag taatgacgtc agacgatgat aatgtttaag gtttacaatg
    12901 cggccataat ctggactttg ttgtatattt tgaccataaa attcacgcaa cgtttttgat
    12961 agggctgtga ttaaatcatc ttttacagat tcgtgtacta aaatgtaatc tggtgcaaca
    13021 catgtttggc cggcattggt gaatttccca aaacaaatgc gctcactggc aactttaata
    13081 ttcgctgttt catcaacgat gactggagat ttaccgccca tttctaatgt cacaggaact
    13141 aaattttcgc tggcagcttg ataaacgatt ttgcctacat tttcacttcc tgtaaagaag
    13201 acatagtcaa aaggtaagtg aattaacgtt tgcgtttctt caattcctcc ttcaataact
    13261 tcaatgtaat ttgcatcaaa tgtttcattg attaatcgtt taatcactcg tgcaacattt
    13321 ggtgtcaact cagatggttt aataattgct gtattacctg ctgcaatagc accgattaaa
    13381 ggttcgaaca ctagttgaaa aggatagtta aatggtgcaa tgatcaaaac tgttccataa
    13441 ggttcttttt tgatatagct ttttgttgga aataaatata aaggtgtgtc tacatttttg
    13501 gttttagtcc agtttttaag ttccttacgg gcatttttga tacttttcaa agttatgcca
    13561 atttcagtag cgtaagcttc gactttattt tttcctaaat ctgtatatag tgcttctaaa
    13621 atatcgctct cgtatgattt aatagctttg cttaacttct ttaattgctc ttttctaaaa
    13681 ctaatatctt tagtttgttg tgtattgaaa aaagctttac tgtcataaaa tttttgctca
    13741 atgatattca taatgaaaag aacctcctta tatgattatt ttggaaaaag tgattaattg
    13801 atttgaatgt tgtggccgtt aattttaaat ggtctttcga attatatatg ttgaaagttg
    13861 aaaatagagc gatgaatcgt gtacataata atatttataa ctttaatcat aacgaaaaag
    13921 gtaggaagaa aacaaaaatt tatactcaac atcgcaaata tttcaagaaa atgtaaaggc
    13981 aaaaggggaa ttgtatagaa atcactaatc tgtggattag gatagctaaa ggaatgaaaa
    14041 ctgctattga aaaggttgta aattagtcaa acgtaaaaaa aacagttcat tgaaagtgaa
    14101 ataaattcta ctttaatgaa ctgttagtta aatacaacat gtctataatt agacagtaat
    14161 atagtattat tttgttaatg cttcagtgat ttgaggtacg atttgttttt ttcgagaaac
    14221 aacaccagat aagaaggcca tgtcatcttc taattgaaca ttgaatgctt cgccaacttt
    14281 atctttttca gcacctacaa ctaaaatttt agaatcacta ttaatgatgt cagtaacaac
    14341 aagtacaaat aagtcatatt tttcttgtgc acttacagct aacatttctt tttctaaatc
    14401 ttctttacga tttaacactt cgtcaaggtc aacagcatta acttgtgcaa tacgagtcac
    14461 atagtcaccc atagtaaatg atttagcatc catgtttaat aagaattcaa ctgatttatc
    14521 agttgttgaa gcacctgctt ttaacatatc taagccgtac ttttgaatat caactttagc
    14581 aatatctttt aattcttcag ctgctttaac atcttgttgt gtacatgttg gtgatttgaa
    14641 aagtaagcta tctgaaataa ttgctgataa cattaaacca gcaatttcag gtttaatttc
    14701 aaagccacgt tctctaaaca ttttgtataa aattgtagct gtacaaccaa ctggttcagc
    14761 acgataacat aaaggaccag cagtttcgaa atttgcaatt ctgtgatgat caattacatg
    14821 cttaattgta gcagaggcaa tcgtatctga actttgttgg aattcgttat gatcaactaa
    14881 gataacatct tggccatcta aatcatccgt taataattcc ggagcaggta cattaaatgt
    14941 atctaacgcg aattgagttt ctgcactcac atcacctaaa cggtatgctt tggctcctga
    15001 attacctcta agttgttcaa attctgccat aataatcgca gatgaaattg catcagtgtc
    15061 tggattctta tgtccgaaaa tatatgtttt agccattgtc aaatatctcc cttgtatatt
    15121 gtattcttta taattatttt agcacgattt aaacgtgttg ttctacctct gcacctaatg
    15181 agtttttgaa atgtgcaagt gcccattcat gccctttttg attaaatgaa gccactccct
    15241 cagcaggtac tgaaatttta tagcctaagt tgtatgcaga aattgctgta tgtaacacgc
    15301 aaatgtcggt acatacacca acgatttcga cttgattaat acttctttct ctcaataaac
    15361 tatcaagcgg ggtaccaaag aacgaatcat agcgcgtttt atcaatgaaa tgtacattag
    15421 gttgcgcttt aattgtttcg tataatttac ctactttacc gtataattct ctaccacttg
    15481 tatctacgat attgtgtggt gggaataatt tactttcagg atgatgaatg tcatgtaaat
    15541 aatgtaaatc cattaaaaag aatatatggt cttgataata attaaagtca ttgatacgag
    15601 aaacaataaa atcttcaata ttttgtccag gtttaccgca tgttagtaag ccgtcgtctg
    15661 cgataaagtc atatgaatag tcaacaacta atagtgcgcg atgtgtcatt caacatctct
    15721 cctatataat aataaaataa actcccatct attcataaat gctagagtat tttttaaccc
    15781 taatggttaa tatataaata tagattaagt atatagatta atcaactttt ttggaagagc
    15841 aaatcacgca atcaacaaat attataagat gtttttacga tagctttaaa atagctgtaa
    15901 tagaatacta aatgtgacaa acttagaact aatatcaagt gttgatgttt tcaatataaa
    15961 aatgctaata gatggcatca cgagatttat gactatctgc taaatcaacc taacacgatt
    16021 ataatttgaa taattggtta atatatgaag taattagaaa atagacaaag gatgacgatt
    16081 tatgtatatc aatatgaaag attatgggtt aacaggcata aacaaaacta aagatactcg
    16141 agcaatacaa cgtgcgttaa atcatggaag atgtaaacca acgacagttt atataccgaa
    16201 agggacgtat gatatttgca aaccattaac gatatatggc aatacaacac ttttgttgga
    16261 taatgaaact attttacgtc gatgtcattc tggtccgtta ttaaaaaatg gtcatcgctt
    16321 tggtttttat cgtggttata atggacacag tcatattcat attaaaggcg gcaagtttga
    16381 tatgaatggc gtatcgtatc cttataataa tacagctatg tgcattggac atgctgaaga
    16441 tattcaatta ataggtgtaa ccattaagaa tgtagtgagt ggtcatgcaa ttgatgcttg
    16501 tgggattaat ggactctata ttaaaaactg ttcatttgaa ggattcgtag actatagtgg
    16561 cgaacgtttt tattctgaag caatacaatt agacattcaa gtacctggtg cttttccaaa
    16621 attcggaacg acagatggta cgataacgaa aaatgtcatt atcgaagatt gttattttgg
    16681 accttcagaa ttgcccgaaa tgggaagttg gaatcgtgct attggctcac atgcaagtag
    16741 acataatcgt tactatgaga atattaatat tagaaataat atatttgaag atatacaagg
    16801 ttatgcatta actcccttga agtataaaga tgctttcatt attaataata agtttattaa
    16861 ctgtgagggt ggtattagat atttaggagt tagagatggt aaaaatgctg cagatgtgat
    16921 gacaggaaaa gacttaggtt cccaagcagg aataaatatg aatataattg gaaatgaatt
    16981 taaagggtca atgtctaaag atgcgataca tgtacgtaat tataataatg ttaaacataa
    17041 agatgtatta atcgttggga atacattcaa taattcgact caatcaattc atttggaaga
    17101 tattgataca gtgtttttaa gtcctgttga agcgggtatt caagttacta caatcaatgt
    17161 agatgaaata aaaaagtaaa aagtttcgca tgacattagg attaagatta ctggataatt
    17221 tttgaaagcg cattcataaa acggtataaa tatgctataa taaacccaat tatctgataa
    17281 aaggggtatt ttgacggtaa tgataataca agatagacaa ctttctatac tctaatatag
    17341 tgagttgaag tagcttgtca taatcatcat gagggggaaa tttatggctt atttcaatca
    17401 acatcaatca atgatatcga aaaggtattt aacattcttt tcaaaatcaa agaaaaagaa
    17461 accgtttagt acgggacaac ttattggact aatattaggt ccattacttt tcctattaac
    17521 attattgttc tttcatccac aagacttacc ttggaaaggc gtctatgtct tagcgattac
    17581 tttatggatt gcgacttggt ggattactga agcaattcct attgcagcaa cgagcttatt
    17641 accaattgtg ttattaccat taggtcacat acttacacca gaacaagtat catccgaata
    17701 tggcaatgat attatctttt tgtttttagg tggatttatt ttggcaattg caatggaaag
    17761 atggaattta catacgagag tagcgttgac aattattaat ttaattggtg caagcacttc
    17821 aaaaatatta cttggattca tggtggcaac aggattctta tctatgtttg tatcgaacac
    17881 tgcagctgta atgatcatga ttccgattgg tttagcaatt attaaggaag cacatgattt
    17941 acaagaagcc aatacgaatc aaacaagtat tcaaaagttt gaaaaatctc tagttttagc
    18001 aattggctat gcaggtacga ttggcggatt aggtacactt atcggaacgc cgccgctaat
    18061 tattttaaaa ggacaataca tgcaacattt tggacatgaa attagttttg ctaaatggat
    18121 gattgtaggg attccaacgg tcattgtatt gttaggtatt acttggctct atttaagata
    18181 tgttgcgttt agacatgatt tgaaatattt acctggtggt cagacgttaa ttaaacaaaa
    18241 gttagacgag cttggcaaaa tgaagtatga agaaaaggta gtacaaacta tctttgtact
    18301 tgccagctta ttatggatta caagagagtt tcttctgaaa aaatgggaag ttacgtcatc
    18361 cgttgcagat ggtacgattg ctatttttat atcaatatta ttatttatta ttccagctaa
    18421 aaatactgag aaacatcgcc gtatcattga ctgggaagtt gcaaaagagc tcccttgggg
    18481 tgtattaatt ttatttggtg gcggtttagc attagcgaaa ggtatttctg aaagtggttt
    18541 agcaaaatgg ttaggcgaac agttgaaatc attaaatggt gtcagcccga ttcttattgt
    18601 aatcgtcata actatctttg tcctattttt aactgaagtg acatctaata ccgcaactgc
    18661 aacgatgatt ttaccgattt tagcaacgtt atctgttgct gttggagtgc atccattact
    18721 acttatggca cctgcagcta tggcggctaa ctgtgcatac atgttaccag tagggacacc
    18781 accaaatgca attatctttg gttcaggtaa aatatctatc aaacaaatgg catcagtagg
    18841 attctgggta aacttaatca gtgcaataat tattatttta gtcgtgtatt atataatgcc
    18901 tatagtttta ggtattgata taaatcaacc actgccattg aaatagtaat tgcagattag
    18961 aacgaaaaat aaaatgttac attagcaatt gcttgaacga gtggtaacga aacgtatacc
    19021 gtagcatcgt gtaaaaacaa tacaaacaaa agaaagtcaa ccaaggatgg attcctattt
    19081 taatccttgg ttgactcttt attttattta aattgtagaa cctagaaaat aaagtttaat
    19141 taaaagcacc aatcatttct actttgaaat ctaaggtttc taaaatagca atgactttct
    19201 ttatatcggt tgtaattgca gaatcagcct gaacgaaaaa tcgatacata cctaattgtg
    19261 tttttaaagg acgagactca atccaggata aattaatatt aaacaaagca aatgtattaa
    19321 gcacacttgc taacaaccca ggtttatcat gcattggtgt aattaaaaac atcaatgatg
    19381 tcgcattttg atcaaattgc tgctgatttt ttataactaa aaaacgtgtc acgttatgtg
    19441 gatagtcttc aatatgtgta tcaat
//
LOCUS       DAMZPP010000039        19327 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMZPP010000039 DAMZPP010000000
VERSION     DAMZPP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19327
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.039"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     misc_feature    43..168
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            197..724
                     /gene="infC"
                     /locus_tag="POH73_001069"
     CDS             197..724
                     /gene="infC"
                     /locus_tag="POH73_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDI6887035.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLISQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     gene            753..953
                     /gene="rpmI"
                     /locus_tag="POH73_001070"
     CDS             753..953
                     /gene="rpmI"
                     /locus_tag="POH73_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDI6887036.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            1000..1356
                     /gene="rplT"
                     /locus_tag="POH73_001071"
     CDS             1000..1356
                     /gene="rplT"
                     /locus_tag="POH73_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDI6887037.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            1498..2106
                     /locus_tag="POH73_001072"
     CDS             1498..2106
                     /locus_tag="POH73_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDI6887038.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKLEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            2125..3054
                     /locus_tag="POH73_001073"
     CDS             2125..3054
                     /locus_tag="POH73_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887039.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLEQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLTDFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNMYSYKLISLMIEYLRFANCTQELTIEKYGMDITFVPANLK
                     GLEHTTLKEKVIPNVMETLNEGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            3059..3169
                     /locus_tag="POH73_001074"
     CDS             3059..3169
                     /locus_tag="POH73_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887040.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            3217..4518
                     /locus_tag="POH73_001075"
     CDS             3217..4518
                     /locus_tag="POH73_001075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDI6887041.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDESQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            4669..5931
                     /gene="clpX"
                     /locus_tag="POH73_001076"
     CDS             4669..5931
                     /gene="clpX"
                     /locus_tag="POH73_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDI6887042.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAITELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            6085..6675
                     /locus_tag="POH73_001077"
     CDS             6085..6675
                     /locus_tag="POH73_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDI6887043.1"
                     /translation="MKVNPNNIELIISAVKEDQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            6892..8238
                     /locus_tag="POH73_001078"
     CDS             6892..8238
                     /locus_tag="POH73_001078"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDI6887044.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WVSMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAKCPHEQAKQQKESKVKEISARRIFSFE"
     gene            8260..9075
                     /gene="ccsA"
                     /locus_tag="POH73_001079"
     CDS             8260..9075
                     /gene="ccsA"
                     /locus_tag="POH73_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDI6887045.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTKHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            9117..10043
                     /gene="hemC"
                     /locus_tag="POH73_001080"
     CDS             9117..10043
                     /gene="hemC"
                     /locus_tag="POH73_001080"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDI6887046.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKKVSNKLKEQGAYEIIKRLNEQH"
     gene            10065..10733
                     /locus_tag="POH73_001081"
     CDS             10065..10733
                     /locus_tag="POH73_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDI6887047.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLESF
                     NQTNQKILLPSSELARPLLSAALSEDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            10736..11710
                     /gene="hemB"
                     /locus_tag="POH73_001082"
     CDS             10736..11710
                     /gene="hemB"
                     /locus_tag="POH73_001082"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDI6887048.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            11758..13044
                     /gene="hemL"
                     /locus_tag="POH73_001083"
     CDS             11758..13044
                     /gene="hemL"
                     /locus_tag="POH73_001083"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDI6887049.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGHFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            13092..13205
                     /locus_tag="POH73_001084"
     CDS             13092..13205
                     /locus_tag="POH73_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887050.1"
                     /translation="MYIRHGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            13239..14312
                     /locus_tag="POH73_001085"
     CDS             13239..14312
                     /locus_tag="POH73_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDI6887051.1"
                     /translation="MTMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKDNWLSIIVVSISILLIALVMA
                     FIFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(14535..15095)
                     /locus_tag="POH73_001086"
     CDS             complement(14535..15095)
                     /locus_tag="POH73_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDI6887052.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAHDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAFGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLKDCPSKPRHN"
     misc_binding    15153..15454
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15498..18128
                     /locus_tag="POH73_001087"
     CDS             15498..18128
                     /locus_tag="POH73_001087"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDI6887053.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNNKAGKYEGMDRFDCRKQLVEDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNGNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGETIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     gene            18141..>19327
                     /locus_tag="POH73_001088"
     CDS             18141..>19327
                     /locus_tag="POH73_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDI6887054.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPI
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDDIQLVDDYVE
                     FIK"
BASE COUNT         7034 a         2640 c         3694 g         5959 t
ORIGIN      
        1 aaatgtgaat tgttgatatg attaacaacg ttgaatacga aacgaaccaa gtagaagcac
       61 ccgcttctca cctgtagcga cgcataaagc agttggcagg ttaatcacat tacacataac
      121 tgtgtattag gaagagcggg tattttatac ctgctcttcc tttttgcttg gtgcaaattt
      181 cgtaaaaatt ttggaggtgt caaccatagc aaaagatcaa actcaaatca atgacaaaat
      241 tcgtgcaaaa gaattacgtt taatcagtca agatggtgaa caaattggtg ttaaatcaaa
      301 gcgtgaagct ttagaaatgg ctgaacgtgt agatttagac ttagtggtcg ttgcaccgaa
      361 tgcgaaacca ccagttgcaa gaattatgga ttacggtaaa ttcaaattcg aacaacagaa
      421 aaaagaaaaa gaaatgaaaa agaaacaaaa aattatcaat gttaaagaaa ttcgtttaag
      481 tccaacaatt gaggaacatg atttccaaac taagttgaaa aacggacgta aattcttaac
      541 taaaggcgat aaatgtaaag tatctattcg tttcagaggg cgtgccatta cgcataagga
      601 aattggtcaa cgtgtgctag aaaaatatgc agatgaatgt aaagatatag caacagttga
      661 acaaaaacct aaaatggacg ggcgtcaaat gtttattatg ttagcgccaa cagctgaaaa
      721 ataattaaag ctattacagg aggacttata ttatgccaaa aatgaaaact caccgcggag
      781 cagctaaacg tgttaaaaga actgcttcag gtcaattaaa acgttcaaga gctttcacat
      841 ctcacttatt cgcaaacaag agcactaaac aaaaacgtca attacgtaaa gctagattag
      901 tgtctaagag cgacatgaaa cgtgtaaaac aattattagc atacaaaaaa taagaacaaa
      961 tacagaaatc ggtaggaatt acctaaggag gaatttttta tgccacgagt taaaggtgga
     1021 acagtaacaa gagcgcgtcg taaaaaaacg attaaattag ctaaaggtta cttcggttca
     1081 aaacatacat tatacaaagt agctaagcaa caagtaatga aatcaggtca atacgctttc
     1141 cgtgaccgtc gtcaacgtaa acgtgacttc cgtaaattat ggattacacg tatcaacgca
     1201 gcagctcgtc aacatgaaat gagctactca cgtttaatga acggtttgaa aaaagctggt
     1261 atcgacatta accgtaaaat gttatcagaa attgcaattt ctgacgaaaa agcatttgct
     1321 caattagtaa ctaaagctaa agatgcttta aaataataac acttataaaa aagagatggc
     1381 attgtcatct cttttttatt ttttaaatag caaaatgcca catttaatcg ctaaaatatg
     1441 acatatttgc taaaatgcat ttaagagaac aaacattaaa gtgaggagtt ttatgcgatg
     1501 tctaaatttg atgaacaaat cattgtagta cctagagaaa ttatttttaa taatgaaaag
     1561 aatactttca atgggttttt aaataagaat aaactagaag gtcaaaatat ttttgatgcg
     1621 ctaagtcaat atgaagttaa gcgacgcggc gatatggaag aggatccatc atataaacaa
     1681 ctcatttctt attgtttact tgaaaatgag catggcgaga tattagtgta tgaacgatta
     1741 tctggcggtg gagaagctcg attgcatgga caatcttcaa taggtgtagg cggtcatatg
     1801 aatgatgttc caggagcaga atctattaac gaagtattga gagttaatgc acagagagaa
     1861 ttagaagaag aagtaggttt aagtgagcaa gattcacaaa atatggaata tatcggtttt
     1921 attaatgacg ataataacga agtgggcaag gtacatattg gtgttgtatt taaaatcact
     1981 gtaagtacga atgatgtaga agctaaagaa acagatactt tacgaataaa atgggttgaa
     2041 aaaggcaaca tagagtcata tgatgatttc gagacgtgga gcgcattaat ccttcaagat
     2101 ttataatcaa acgaggtgac atatatgtca gatattattc agtttcctaa ctcttcgaaa
     2161 aagttatata aagatataaa acgtgctgaa caagaccaaa actatgacct gatgtatgaa
     2221 tatattgtac aatatgaacg tcaatttgaa ctcactgaag aaatagcaat gatgaaatgc
     2281 cgtatgttat atgagacggg gtcatttctt gaattaagag aagaaacaat tgtcttattg
     2341 aaaactggca tacaacaata tgatgcattg atgatttatt atgtgaaaag tttgataggt
     2401 ttggaacaat attttgaagc ggtagaagta attcatcaaa ttattgatga ggtcaaagat
     2461 cacaaaacaa gaatggcatt acatcctctt aaagaatttg ctaagtcaaa attaattgaa
     2521 gatgaaaaac gattgacaca gtcattaact gattttgata cgttatcaat gagggaacag
     2581 acgcacttga ttttgaagtt aatagacaat ggtcattttc aatttcaaga aacggtatta
     2641 tatatattga aatctaatat gtacagttat aaactaatta gtttaatgat tgagtattta
     2701 aggttcgcaa attgtacaca agaactgaca attgaaaagt atggtatgga tataactttt
     2761 gtaccagcta atttaaaagg gctagaacat acaacactga aagaaaaagt tatacctaac
     2821 gttatggaaa cattaaatga aggtgcttta catatcgctg aagaagcaca ccatattatg
     2881 aataatcact ctatcatgat ttatccaatt gatattgaaa ctttgtttga aacgaataaa
     2941 tggattaatg catacgaatg ttatttcaaa aatatgttag gacttaaatg tgaattacaa
     3001 agtatcgatg catttaattt tattcagcaa ttagatttga ataataatag ttaaattgtt
     3061 gcaaaataat actcagcatt ttaatatagt tttgacgaaa aaagttgatt tacaaatata
     3121 taaacgttgt gatttcaatg tttgtatagg gaatagacct atgatataat agggaagttg
     3181 aaaaaataaa ttagttaatc atggaggtat atatacatga cagcaacttg ggaaaaaaag
     3241 gaaggtaacg aaggtttatt aacagttact gttcctgcag aaaaagtaaa caaagcgtta
     3301 gatcaagcat ttaaaaaagt ggttaaacaa attaacgtac ctggattccg taaaggtaaa
     3361 gtgccacgtc caattttcga acaacgcttt ggtgtagaag cattatatca agatgctatc
     3421 gacattttat taccagatgc ttatggtgaa gcaattgacg aaactgatat taaaccagtt
     3481 gcacaaccag aagtaagtgt tactcaaatt gaaaaaggta aagatttcat ttttgaagca
     3541 acagttacag ttgagccaga agttaaatta ggagactata aaggtcttga aattgaaaaa
     3601 caagaaactg aattatctga tgatgagtta caagaagcaa ttgaccacag cttaggacat
     3661 ttagctgaaa tggtagttaa agaagatggt gttgttgaaa atggcgacac agttaacatc
     3721 gactttagtg gttcagttga cggagaagaa ttcgaaggtg gacaagctga aggttacgat
     3781 ttagaaatcg gttcaggttc attcattcct ggtttcgaag agcaattaga aggtatgaaa
     3841 gttgacgaag aaaaagatgt tgtcgtaaca ttcccagaag aataccatgc tgaagaatta
     3901 gctggtaaag aagcaacttt caaaacaaaa gttaacgaaa ttaaatttaa agaagttcca
     3961 gaattaactg atgaaattgc taatgaatta gatgcagaag caaatacagt agacgagtac
     4021 aaagaaaact tacgtaaacg tttagctgaa caaaaagcta cagatgcaga aaatgttgaa
     4081 aaagaagaag cgattactaa agctactgat aatacaacaa tcgatattcc tgaagcaatg
     4141 gttaatactg aattagatcg tatggtgtct gaatttgcac aaagaattca acaacaaggt
     4201 ttagatttac aaacgtactt ccaaatctca ggtcaagatg aatctcaatt aagagagcaa
     4261 atgaaagacg atgcagaaca acgtgttaaa actaacttaa ctttaactgc aatcgctgaa
     4321 gctgaaaaaa tcgaagctac tgatgaagat atcgataaag aattagaaaa aatgagtaaa
     4381 caatttaata tctcagttga agatatcaaa aatactttag gtaatactga tatcattaaa
     4441 aatgatgttc gtattcaaaa agttatcgat ttattaagag ataacgcaaa gttcgttgaa
     4501 ggaactaaag aagattaatc ttcattaaat attaaattac aaaaatgagt agcagatgca
     4561 tagcttatgt atctgctact attctttaag caaaaagttt gtatgttaat atgttgcatt
     4621 gtaatatcca atctagtata gtctttaacg aataggggtg taaaaagaat gtttaaattc
     4681 aatgaagatg aagaaaattt gaaatgctct ttctgcggaa aagaccaaga tcaagtaaaa
     4741 aaacttgtag caggaagtgg tgtatatatt tgtaatgagt gtattgaatt atgctcagaa
     4801 atcgtcgaag aagaattagc tcaaaacact tctgaagcga ttacagaatt acctactcct
     4861 aaagaaatta tggatcattt aaacgaatat gttattggtc aagaaaaagc taaaaaatct
     4921 ttagctgtgg ctgtttataa ccactataag cgtattcaac aattaggacc aaaagaagat
     4981 gatgttgagt tacaaaaaag taacattgcc ttaattgggc caacaggtag tggtaaaaca
     5041 ttattagctc aaacattagc caagacgttg aatgtaccat ttgcaattgc agatgcgaca
     5101 agtttaactg aagctggtta tgtaggcgat gatgttgaaa atatcttgtt gagattaatt
     5161 caagcagctg actttgacat tgataaagcc gaaaaaggta ttatttatgt agatgaaatt
     5221 gataaaattg cacgtaaatc tgaaaacaca tctataacac gtgacgtttc aggtgaaggt
     5281 gttcaacaag cattgcttaa aatcttagaa ggtacaactg caagtgttcc gccacaaggt
     5341 ggacgcaaac atccaaacca agaaatgatt caaattgata caacaaatat cttatttatt
     5401 cttggtggtg cctttgatgg tattgaagaa gtgattaagc gccgtcttgg tgaaaaagtt
     5461 attggtttct caagcaatga agctgataaa tatgacgaac aagcattatt agcacaaatt
     5521 cgcccagaag atttgcaagc ctatggtttg attcctgaat ttatcggacg tgtgccaatt
     5581 gtagctaatt tagaaacatt agatgtaact gcgttgaaaa acatcttaac gcaacctaaa
     5641 aatgcacttg tgaaacaata tactaaaatg ctggaattag atgatgtaga tttagagttc
     5701 actgaagaag ctttatcagc aattagtgaa aaagcaattg aaagaaaaac aggtgcgcgt
     5761 ggtttacgtt caatcataga agaatcgtta atcgatatta tgtttgatgt gccttctaac
     5821 gaaaatgtaa cgaaggtagt tattacagca caaacaatta atgaagaaac tgaaccagaa
     5881 ttatacgacg cagaaggtaa tttaattaat aatagtaaaa catcagctta atcattgatg
     5941 tgttataaaa gtgaaaagct ccaatcaaag ttgacgctca aatagtttaa ctttgatcgg
     6001 agctttttta taatgtatac cgaaaggttc aaatattata taatggtaaa gttgaaagag
     6061 aatataaaaa agtgaggata taaaatgaaa gttaatccta ataatataga attaatcatt
     6121 agtgcagtaa aggaagatca atatccagaa acagaattgt ctgaagttgc actgagcggt
     6181 cgatctaatg taggtaagtc tacatttatc aatagtatga ttggcagaaa aaatatggca
     6241 cgtacatcac agcaacccgg caaaacgcaa acgttaaatt tttataatat agatgaacaa
     6301 cttatttttg tggatgttcc agggtatgga tatgctaaag taagtaaaac acaacgtgaa
     6361 aaatttggga aaatgattga ggaatatata actaagagag agaatttaca attagttatt
     6421 caattagttg atttaagaca tgatccaaca caagatgata tcttaatgta caattatttg
     6481 aaacattttg atattcctac tttagttata tgcactaaag aagacaaaat tccaaaaggc
     6541 aaagttcaaa agcatattaa aaatattaag acacaattag atatggaccc agacgataca
     6601 attgtaagtt attcatcaat tcaaaataat aaacaacaac aaatatggaa tttaattgaa
     6661 ccgtatattt catagttttt gtacgacaaa acttatacaa aaattttaaa aataatgtaa
     6721 gcacgaaact tttaattagt acacaattga taacattttt caacgttcat cattttgtca
     6781 aaaactcaaa agtaaattag aaagattata atttatttaa gcatcgtact taattggatt
     6841 ttaaattatg ttataatatt tgtattgtta gtatatatgg gggcgtttca aatgcatttt
     6901 attgcaatta gtataaatca tcgcacagct gatgttgcac taagagagca agttgctttt
     6961 agagatgatg ccttacgaat tgcccatgaa gatttatatg aaactaaatc tattttagaa
     7021 aatgtcatat tatcaacatg taatcgaact gaagtatatg ctgttgttga tcaaattcac
     7081 acaggtcgtt actatattca acgatttcta gctcgtgcat ttggatttga agtagatgat
     7141 attaaagcaa tgtcagaagt aaaagtgggg gacgaagcag tagaacattt attgcgtgtc
     7201 acttctggtt tagattcaat cgtacttgga gaaactcaaa ttttaggtca aataagagat
     7261 gcatttttct tagcgcaaag cacaggtacg acaggaacaa tttttaatca tctatttaaa
     7321 caggcaatta cttttgcaaa aagagcacat aatgaaacag atatagctga taatgctgta
     7381 agtgtgtctt atgctgcggt cgagttggcg aaaaaagtat ttggcaaatt gaaaagtaag
     7441 caagctatca ttattggtgc aggggaaatg agtgaattat cactattaaa tcttcttggt
     7501 tctggaatta ctgatattac agtagtaaat agaacaattg aaaatgctat gaaattagca
     7561 gcaaagcatc aagtgaaata tgatgaacta tcatcattac caaatttact tgaaagtgca
     7621 gatattgtga ttagttcgac gagtgcacaa tcttatatca ttacaaatga aatgatagaa
     7681 agaattgcag aaaatagaaa gcaagattca ctagtattga ttgatattgc agttcctcga
     7741 gatattgaac ctggtattag tgccatcaca aacatcttta attatgatgt tgatgactta
     7801 aaaggtttag ttgatgcaaa cttacgtgag cgacaattag cggctgcaac aatttcggaa
     7861 caaattcctg cagaaataca tgcacacaat gagtgggtta gtatgttggg tgttgttcca
     7921 gtgattagag ctttacgtga aaaagctatg gcaattcaag cggaaacgat ggacagtatt
     7981 gatcgtaaat tgccagggtt aagcgaaaga gaacgtaaaa ttatatctaa acatacaaaa
     8041 agtatcatca atcaaatgtt gaaagatcct attaaacagg ccaaagaatt aagtagtgat
     8101 aagaaaagta atgaaaaatt agagctattt caaaatatat ttgatatcga agctaaatgt
     8161 cctcatgagc aagcgaagca acaaaaagaa agcaaggtca aggaaatttc agcgcgacgt
     8221 atctttagtt ttgaataagc atattaatat ggtgataata tgcaagaaaa cctgtttatt
     8281 cgattcaatg aaattatatt attaatatac ttaatcagta tcatttgcta tttttatgat
     8341 tttgtccaaa aaagtcataa gattagaagt ttaggcatat atttattggg gattgtttgg
     8401 gttttacaaa caatctcttt atctattttt attatacaaa ctaaacatat tccattaggg
     8461 tctatttcag atgtattcta tactttaagt tggctgatta tttcaatctc tttaattctt
     8521 aaccttatca aagttttgaa tttctctgtg tttttcttga atttaatagg gttaacactt
     8581 ttagggatga atacatttca accgacacat tatactaata aggttcagaa aattgctgtt
     8641 gtagacgagt tactgttagt ccatattggt ctagctgtgt taagttatgt cttctttgcc
     8701 ttagcttttg tcaatgcatt actgtatatc atgcaatacc gtaatttgaa ggaaaagcgt
     8761 tttgatcaaa aatactttag aataggtagc gttgctacac ttgaatcaat tgtgttttat
     8821 tcaacgttaa gtgggtggat aattctcata tttagtatta ttttaggtac acaatggggt
     8881 gtaatttcag ttggtgaacg tatattcatt gacccgaagg taatactctc ttcaattatt
     8941 acagtattat atggaagtta tattttactt agaatcaaca aatggttgaa ttcaaggtat
     9001 ttaatatact ataacattat tttattttgc ttaaatatga ttaatttatt ttttgcaaca
     9061 cattttgtga attaagaaaa taatctagag caatccgttc ctaggaggaa ttgaacatgc
     9121 gtaaattagt cgttggctcc agaagaagta aattagcttt aacacaaagc cagcaattta
     9181 ttgataaatt aaaagctgtc gagccaaatc tagaaattga aattaaagaa attgtcacga
     9241 aaggcgatcg tatagtagat aaacaattgt ctaaagtcgg aggcaaaggc ttatttgtta
     9301 aagaaataca acatgaactt tttgaaaaaa atatcgatat ggcaatacac tcgctgaaag
     9361 acgtaccaag tgtaattccg gaaggtttaa cattaggttg tatccctgat agagaattac
     9421 cttttgatgc gtatatttct aaaacacata caccactatc ccaattgcca gaaggtagta
     9481 ttattggtac tagttcatta cgtcgtggtg cacaaatatt atctaagtat cctaatttag
     9541 agattaaatg gattagaggt aatatagata cacgattaga aaagttacaa actgaagatt
     9601 atgatgcgat tattttagct gcagctggtt taagaagaat gggctggtca gatgatattg
     9661 taacatctta tcttgataga gatacattgt tacctgcaat tggacaaggt gctttaggga
     9721 tagaatgtcg tagtgacgat gaagaactat taacattatt aagcaaagta cataatgatg
     9781 aggttgcaaa atgtgtgact gctgaacgaa cgtttttagc agaaatggat ggtagttgtc
     9841 aggtgccaat cgcaggatat gctacaatct cagatcaaaa agaaatcgaa tttacaggtt
     9901 taattatgac cccagatggt aaagaacgat ttgaatatac aatgaacgga acagatccgg
     9961 ttgagttagg aaaaaaagtg agtaacaaat taaaagagca aggtgcttat gaaattataa
    10021 aacgcttaaa tgaacaacat taaataatgg agtgacgttt aaatatgaag ccagttgtag
    10081 ttatgacaca aacaaatgac atgcaaagtg atttagtatc aattattcac aaacctttta
    10141 ttgatataaa gccactaaat tttgatattc atttgttaaa tcaacgctat gactggctta
    10201 ttttttcatc taaaaatgct gtgaaattct tttataaata tctaaaagga attaatgttg
    10261 ataacattgc tgtgatagga agtaagacag cgcaatattg tgaatcactt ggcattcgag
    10321 ttgattttat gccaaacgac ttttctcaag aaggattttt agaatcattt aatcaaacta
    10381 accaaaaaat acttttgcct tcgagtgaat tggcgagacc attgttatca gcagcgttat
    10441 ctgaagataa tgaagttgtt aaaatagatt tatatacttc agtgcctaac aaacaaaata
    10501 tacaagatgt taaagaaatg atagaacatc aacaaatcga tgcattaaca ttttcaagtt
    10561 cgtcggcagt acgttattat tttaatgaag gatttgtacc aaaattcaag tcgtattttg
    10621 ctattggaga acaaacagca cggaccatta aatcatatca acagccagta acaattgcag
    10681 aaattcaaac actcgaatca ctaattgaaa agattttaga aagtaggggc taaaaatgaa
    10741 atttgataga catagaagat tgagatcatc agcgacaatg agagatatgg ttagagagaa
    10801 tcatgtaaga aaagaagatt taatatatcc aatttttgta gttgaaaaag acgatgtgaa
    10861 aaaagaaatt aagtcattgc caggtgtata ccaaatcagt ttgaatttac ttgaaagtga
    10921 attaaaagaa gcttatgact taggcatacg tgccattatg tttttcggtg ttccaaactc
    10981 aaaagatgat ataggtactg gtgcatacat tcacgatggt gttattcaac aggcaacacg
    11041 tattgctaaa aaaatgtatg atgacttatt aattgttgca gacacttgtt tatgtgaata
    11101 tactgatcat ggtcattgtg gtgtgattga tgaccataca catgacgttg acaatgataa
    11161 atcattgcca ctacttgtta aaacagcaat ttctcaagta gaagctggtg ctgatattat
    11221 tgcaccaagt aatatgatgg atggttttgt tgctgaaatt cgtcgtggat tagatgaagc
    11281 cggctattac aatattccta taatgagtta tggtgtcaag tatgcatcaa gtttcttcgg
    11341 accttttaga gatgcagcag attcagcgcc atcatttggg gatagaaaaa cgtatcagat
    11401 ggaccctgct aaccgtttgg aagcacttcg tgaattagaa agtgatctta aagaagggtg
    11461 cgacatgatg attgttaaac ctgctctaag ttatttagat atagttcgag acgttaaaaa
    11521 tcatacgaat gttccagttg ttgcatataa tgtgagtgga gaatatagta tgactaaagc
    11581 agcggcacaa aatggttgga ttgatgaaga acgtgtcgtt atggaacaaa tggtttcaat
    11641 gaaacgtgca ggtgctgata tgattattac gtattttgca aaggacattt gtcgctattt
    11701 agataaataa ggttttatat gtatgatttt ccataaacta taggaggaat ttactttatg
    11761 agatatacga agtcagaaga agcaatgaag gttgctgaaa ctttaatgcc tggtggtgta
    11821 aatagtccag tacgcgcatt taaatcagta gatacaccag caatttttat ggatcacggt
    11881 aaaggctcaa aaatttatga tatcgatggt aacgagtata tcgactatgt actaagttgg
    11941 gggccactta ttttaggaca tagagaccct caagttatta gtcatttaca tgaagcaatt
    12001 gataaaggta caagttttgg tgcatcaaca ttacttgaaa ataaattggc gcagctcgtt
    12061 attgaccgag taccttcaat agaaaaagtg cgtatggtgt catctggtac agaagctaca
    12121 ttggatactt taagattagc acgtggttat actggcagaa ataaaattgt aaaatttgaa
    12181 ggttgctatc atggtcatag tgattcgtta ttaatcaaag ctgggtcagg ggtggcaaca
    12241 ttaggattgc cggattctcc tggtgtgcct gaaggtattg ctaaaaatac aattacagtt
    12301 ccatacaatg atttagatgc acttaaaatc gctttcgaaa aatttggaga cgatattgct
    12361 ggtgtaatcg tagaacctgt tgctggtaat atgggtgtcg taccgccgat tgaaggtttt
    12421 ttacagggat taagagatat tacgactgaa tacggcgcat tgctaatttt cgatgaagta
    12481 atgactggtt tcagagtcgg ttatcattgt gcacaaggtc actttggtgt gacaccagat
    12541 ttaacttgct taggaaaagt tatcggtgga ggactaccag taggtgcttt tggtggtaaa
    12601 aaagaaatca tggatcatat agcaccatta ggaaatattt atcaagctgg tacgttatca
    12661 ggaaatcctc ttgcaatgac aagtggttat gaaacgttaa gtcaattaac gccagagaca
    12721 tatgagtatt ttaatatgtt aggcgatata cttgaagacg gtttaaagcg tgtatttgct
    12781 aaacacaatg taccaataac tgtaaataga gcaggttcaa tgattggtta tttcttaaat
    12841 gaaggacctg taactaattt tgaacaagcg aataaaagtg atttgaaatt atttgcagaa
    12901 atgtatcgtg aaatggcaaa agaaggtgtg tttttaccac catctcaatt tgaaggtaca
    12961 ttcttatcta cggcacacac gaaagaagat attgaaaaaa cgattcaagc atttgatacg
    13021 gctttaagtc gtattgtaaa ataaatatac ggacaaattg agagcctgaa ctttgttcag
    13081 gctcttttta aatgtatatc aggcatggcg gcgacttgat agtgaaagtc catatatgct
    13141 ttgtagtcaa aactgctagc ggatattgtt atcttaacaa acgtgaagct caagcagcaa
    13201 tttagtcatt ttatttttta ttgaaagaag tggaaaacat gacaatgata tatagaaata
    13261 atttcattgt gttcgtttta tcatttttta ttagtattct actgtattca tcgcacgtat
    13321 tactcccatt tatgtttggt cctattatcg catcaatcat ttgtgtaaaa gttttcaaac
    13381 ttgatattaa atggccattc ttacttagtg aattaggaat tgtactatta ggtgtgcaaa
    13441 tcggatcaac gtttacgaaa aatgtcgtta tggatattaa agacaattgg ctttcgatta
    13501 ttgtagtatc tatttcgata ttattaattg ccttagtaat ggcatttatt tttaaaaaaa
    13561 ttgcacgtat taatacagaa acagctattt taagtgttat accaggagca ctaacacaaa
    13621 tgttggtcat ggctgaacaa gacaaacgtg ctaatttgtt agttgttagc ttaacgcaaa
    13681 catcacgaat tatatttgtt gttgttttag taccgttcat ttcatatttt tttcatgatg
    13741 gtaacatgca tgcgaatggt aagttaacaa aagtcttgcc tttatcacaa gtattaaaca
    13801 tagggcaaat agttatttta gcgatagcta tctttatagt ttatctaatt atgtctaaaa
    13861 taaagtttcc aacatttcaa ttattagcac cactcattgt attaattgtt tggaattttt
    13921 ctacaggtct aacatttaca ctagatcatt ggttgttgaa catggcacaa ctaatatata
    13981 tgattagaat tggagttcaa attgcgcatt tattgtcaga tttaaaaggt agactagcaa
    14041 tcgcaattac aattcaaaat attatgttga taattggtgc gctaatcatg gtttatatca
    14101 tacatttctt tgataataat cctattaatg aattgtttct tggtgcagca ccaggagggg
    14161 ttaatcagat agttttagtt gcattagcta ctggtgccga tgttgctatg attacgagtt
    14221 atcatatttt ccgaatcttt ttcatcctat ttattattgc accaggaatt aattactttt
    14281 taaaatatcg ttcgaataag cgtgaagatt agaaactatc atccatttaa gttaaattat
    14341 gcttaggata gaattcatta ttaaggttta agcaaaggtt ttacatctat ttttaaatta
    14401 agatatctaa tattagaagt gcattaacat ggaactttaa cttgtgattt gaataaattt
    14461 ctacattaaa aaacgagaca gtctattagt taaaaataga tgatgtctcg tttactttat
    14521 gatgctatag caaattaatt gtgtctaggc tttgatggac aatcttttaa atgtgcatca
    14581 tataaaccgg ctgcctctaa aaacgaaaat actgttactg gacctaaaaa tttgaacccg
    14641 tattgtttta aatctttaga tagttgtgtt gcagtatcat caactgtgat acgatcagaa
    14701 gcatgttcat actgcaaatc tttaggctta ccatttacat aagaccataa aaatttacta
    14761 aaactaccaa atgcttgttc aatttttaaa tacccttgag cttgattaac aattgcttct
    14821 aattttttac gatgatgaac gatatttgga aaagtcatta agcggtcgat atcatgagcg
    14881 gtcatttgtg ctaccttttc tggttcgaaa tcataaaatg cttcttcata ggcttctttc
    14941 ttttttaaaa tagttaacca agatagccca gcatgttgtg attctaatgc taaaagttta
    15001 aacaatgcct tgctatcata gagcggttgt ccccatacat gatcatgata gtctaagtag
    15061 actggatctt tagttccaaa tgcgcattca ttcattttat tcccacctat acattgactt
    15121 attttgtcaa tcattatact ataagttaag agtttaatat aggatttggg aagagtaagt
    15181 tcaaattatt ttaaagagag catatgattt ggtgtgagta tgtaaatgat ttttactgaa
    15241 ggtagcccac agagtatctt agctgaacgt gattaatagt aggctaagac gtgtttgagc
    15301 gttaatcata atctagagtg cttgaagaga tgaagcattt caaatttcac gatgatagta
    15361 ttggtatgat tattccaatt atgttaaaag agtgaatttg atattaatca tccttcttga
    15421 atataggtgg taccacggaa tatccgtcct atttgtatat aggatggata ttttttattt
    15481 tttaggaggt atagcaaatg gaaatgaaac caaaatatga tcctcgtgaa gttgaagcgg
    15541 gacgttatga agaatgggtg aagaatggtt attttaaacc gtcagaagat aaatcaaaag
    15601 aaacatatac aattgttatc ccgccaccaa atgtaactgg taaattacat ttaggacatg
    15661 catgggatac aactttacaa gatatcatta cacgtatgaa acgtatgcaa ggatacgata
    15721 cgttatactt accaggtatg gatcatgctg gtattgcgac acaggcaaag gtagaagcta
    15781 aattaaatga acaaggaata actagatatg atcttggtcg tgaaaagttt ttagaacaag
    15841 catgggattg gaaagaagag tatgcgtcat ttattcgtgc gcaatgggct aaattaggtc
    15901 taggtttaga ttatagtaga gaacgtttta ctttagatga aggtttaagt aaagcagtta
    15961 aaaaagtttt tgttgattta tacaataaag gaattattta tcgtggcgaa cgtattataa
    16021 attgggatcc taaagcacgt acagctttat ctgatattga agtaatacat gaagatgttc
    16081 aaggtgcgtt ttatcatttt aaatatcctt acgctgatgg tgaaggtttt attgaaattg
    16141 caactacaag accagaaacg atgttaggtg atacagcgat tgttgttaac cctaatgacg
    16201 aacgatacaa agatgtaatc ggtaaaactg ttatattacc aatcgtagga cgcgaactgc
    16261 ctattttagc agatgaatat gttgatatag acttcggttc tggtgctatg aaagtgacac
    16321 cagcacatga ccctaatgat tttgaaattg gtcaaagaca tcaattagaa aatattatcg
    16381 ttatggatga aaatggtaaa atgaacaaca aagctggtaa atacgaaggt atggaccgtt
    16441 ttgattgtcg taaacagcta gttgaagatt taaaagaaca agatttagtt atcaagattg
    16501 aagatcatgt tcattctgta ggtcattcag aacgatctgg cgctgttgtt gaaccatatt
    16561 tatcaacgca atggtttgtg cgcatggaag acttagcgaa acgttcatta gataaccaaa
    16621 aaacagatga tcgtattgat ttttatccgc aacgtttcga acatacattt aaccaatgga
    16681 tggaaaatat tagagattgg acgatttcaa gacaattatg gtggggtcat caaattccgg
    16741 cttggtatca taaagaaaca ggagaaatat atgttggaga agaagcgcca actgatattg
    16801 aaaattggca acaagatgaa gatgtattag atacgtggtt ctcaagtgct ttatggcctt
    16861 tctctacgtt aggttggcct gatttagaaa gcgaagactt taaacgatac tacccaacaa
    16921 atgccttagt tacaggttac gacattatct ttttctgggt agcacgtatg atattccaag
    16981 gcttagaatt tacagatcgt cgtccattta atgatgtgtt attacacggc ttagttcgcg
    17041 ctgaagacgg gcgtaagatg agtaaatcat taggtaatgg tgtggatcca atggatgtta
    17101 ttgacgaata cggtgctgat agcttgcgtt acttcttagc aacgggttca tctccaggac
    17161 atgatttaag atactcaact gaaaaagttg agtcagtgtg gaactttatc aataaaattt
    17221 ggaatggagc acgtttcagt ttaatgaata tcggtgaaga ctttaaagtt gaagatatcg
    17281 atttaagtgg taacttatca ttagcagata aatggattct aacacgttta aatgaaacga
    17341 ttgcaacagt tactgatcta agtgacaaat atgaattcgg cgaagttgga cgagcattat
    17401 ataatttcat ttgggatgat ttctgtgatt ggtacattga aatgagtaaa attccaatga
    17461 atggtaatga tgaagaacaa aaacaagtta cacgttcagt attgagttat actttagaca
    17521 atattatgag aatgctacat ccattcatgc catttgtaac agagaaaata tggcaaagtt
    17581 taccacatga aggtgaaaca attgttaaag cttcatggcc agaagtgcgt gaatcattga
    17641 tttttgaaga aagtaaacaa acaatgcaac aacttgttga aatcattaaa tctgtaagac
    17701 aatcacgtgt agaagtaaat acgccattgt ctaaagaaat acctatttta attcaagcta
    17761 aagataaaga aattgaaaca actttatcac aaaacaaaga ttatttaatc aaattctgta
    17821 atcctagtac gttaaatata agcactgacg tggaaattcc tgagaaagca atgacatcag
    17881 ttgtaattgc aggtaaagtg gtattaccat tagaagggct aattgatatg gataaggaaa
    17941 tcagccgttt ggaaaaagaa ttagctaaac ttcaaagcga attagataga gtagataaaa
    18001 agctctctaa tgaaaacttt gtaagtaaag cacctgaaaa ggttataaat gaagaaaaac
    18061 gcaaaaaaca agattatcaa gaaaaatatg atggtgtgaa ggcaagaatt gaacaattaa
    18121 aagcatagga gttagtaaca atgaattacc tagagagctt gtattggata cacgaaagaa
    18181 ctaaatttgg catcaaacca ggtgttaaac gtatggaatg gatgctagca caatttaata
    18241 atcctcaaaa taacattaag ggtattcatg taggtggcac gaatggtaaa ggctctacag
    18301 ttgcttacct tagaacagct ttagttgaaa atggttatga agtaggtaca tttacgtcgc
    18361 cgtttattga aacatttaat gaacgaatta gtctaaatgg tgtgccaata tcaaatgacg
    18421 ctattgtaga attagtatca cgtattaaac cagttagtga aatgatggaa cgtgaaacag
    18481 atttaggtgt tgcaactgaa ttcgaaataa tcacagcgat gatgttttta tattttggtg
    18541 aaatacatcc tgttgatttt gtcattgttg aggctggatt gggtataaag aacgattcga
    18601 caaatgtctt tacaccgatt ttatcaatct taactagtat cggtctagac catacagata
    18661 ttttaggtgg tacttatcta gatattgcta gggataaagg cgcgattata aagcctaacg
    18721 ttccagttat atatgctgtt aaaaatgaag atgcattaaa gtatgttcgt gaacgcgcaa
    18781 ttgaacaaca tgcaaagcca attgaattag atagagaaat tgttgttgta tcgcaaaatg
    18841 atgaatttac ttaccgttat aaagattatg aattagaaac aatcatttta agtatgttag
    18901 gtgaacatca gaaacaaaat gctgcattag ccataacagc tcttattgaa ttaaatgaac
    18961 aaggattaat tgaattagat ttcaataaga tgatagacgg tattgaatca gttcgttgga
    19021 ctggacgtat tgagcaggtg catgacaaac ctttaatcat tttggatggc gcacataatt
    19081 cagagagtat agatgctcta attgatacaa ttaaacagta ccatgataaa gaaaaagtag
    19141 atattttgtt ctcagcaata aacggaaaac cgattaacga gatggtcaaa catttaagtt
    19201 taattgcgca tacattttat gcaactgaat ttgattttcc gaaagcgtta cgcaaagaag
    19261 aaattgtagg tagcattgaa aatgacgata tacaattagt agatgactac gtagaattta
    19321 taaaaaa
//
LOCUS       DAMZPP010000040        19282 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMZPP010000040 DAMZPP010000000
VERSION     DAMZPP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19282
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.040"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..700)
                     /locus_tag="POH73_001089"
     CDS             complement(<1..700)
                     /locus_tag="POH73_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDI6887055.1"
                     /translation="MNYQVLLYYKYTTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEED
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGQY
                     WDGKMYVFDDRISVD"
     gene            944..1915
                     /locus_tag="POH73_001090"
     CDS             944..1915
                     /locus_tag="POH73_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDI6887056.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKVNPETKEIKRPFVSHKANPAAIKIHKDGRLFICY
                     LGDFKSTGGIFAATENGDNIQDIIEDLSTEYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVTPDFKTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPSRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMDGGSMLYTVNGFAKGHKSYQFQ"
     gene            complement(2061..2576)
                     /locus_tag="POH73_001091"
     CDS             complement(2061..2576)
                     /locus_tag="POH73_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HDI6887057.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSITESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGIINREKYGINFNQTLETGGVMLGKDVK
                     FEASAEFSISE"
     gene            2958..3806
                     /locus_tag="POH73_001092"
     CDS             2958..3806
                     /locus_tag="POH73_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HDI6887058.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEISEATKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            3835..4389
                     /locus_tag="POH73_001093"
     CDS             3835..4389
                     /locus_tag="POH73_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HDI6887059.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLIGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMIYFNTGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            4442..6154
                     /locus_tag="POH73_001094"
     CDS             4442..6154
                     /locus_tag="POH73_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6887060.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNVTGELHINNQDATVSCLHKRSNVVGTVLQDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVDMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLREVCMS
                     EHVKFKVKKWIDKTSSNDDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNKGFNQLSISERSEFIGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLIQLFINHDRK
                     VRRQ"
     gene            6151..6984
                     /locus_tag="POH73_001095"
     CDS             6151..6984
                     /locus_tag="POH73_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDI6887061.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISVSSILLFKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELI
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     H"
     gene            7031..7528
                     /locus_tag="POH73_001096"
     CDS             7031..7528
                     /locus_tag="POH73_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6887062.1"
                     /translation="MINTERLNLMIPSSSHLIDLYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWQNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLNFKRDDTMDDIINQGD
                     IVFYK"
     gene            7646..8770
                     /locus_tag="POH73_001097"
     CDS             7646..8770
                     /locus_tag="POH73_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDI6887063.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAMHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKVKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDSKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLDGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDKAGFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRQFFKGNRDYEE"
     gene            9074..9892
                     /locus_tag="POH73_001098"
     CDS             9074..9892
                     /locus_tag="POH73_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDI6887064.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSLVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHT
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            9910..10524
                     /locus_tag="POH73_001099"
     CDS             9910..10524
                     /locus_tag="POH73_001099"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDI6887065.1"
                     /translation="MLRIAIAKGRLMDSLINYLDAIEFTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGIADIGIVGSDILDERHYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELACVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            10517..11767
                     /locus_tag="POH73_001100"
     CDS             10517..11767
                     /locus_tag="POH73_001100"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDI6887066.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQMNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVNQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDDTADLDAIVYDVFAQAEHDELARTYVISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFDQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            11783..12796
                     /locus_tag="POH73_001101"
     CDS             11783..12796
                     /locus_tag="POH73_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDI6887067.1"
                     /translation="MIYIDKNESPVTPLDQKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPVLTLNPDFFMYQAYAAQVNREIAFVDA
                     RSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKAINGYFVID
                     EAYLDYGKAYDVEMAPHILRMRTLSKAFGIAGLRLGVLMSTVETIQRIQTIEHPYPLN
                     VFTLNIATYIFRHREETSRFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            12765..13343
                     /gene="hisB"
                     /locus_tag="POH73_001102"
     CDS             12765..13343
                     /gene="hisB"
                     /locus_tag="POH73_001102"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDI6887068.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYISMDETLAR
                     VVVDISGRPYLSFNATLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            13340..13918
                     /gene="hisH"
                     /locus_tag="POH73_001103"
     CDS             13340..13918
                     /gene="hisH"
                     /locus_tag="POH73_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDI6887069.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNKQNIIDQAETIILP
                     GVGHFKDAMSEIKRLNLDAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGTDIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            13911..14615
                     /locus_tag="POH73_001104"
     CDS             13911..14615
                     /locus_tag="POH73_001104"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDI6887070.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTEWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVKQLSDI
                     PIGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            14612..15370
                     /gene="hisF"
                     /locus_tag="POH73_001105"
     CDS             14612..15370
                     /gene="hisF"
                     /locus_tag="POH73_001105"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDI6887071.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTHGGKKLTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEAIRQGGIAVR"
     gene            15367..15999
                     /locus_tag="POH73_001106"
     CDS             15367..15999
                     /locus_tag="POH73_001106"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDI6887072.1"
                     /translation="MTNYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            17090..19132
                     /gene="lip1"
                     /locus_tag="POH73_001107"
     CDS             17090..19132
                     /gene="lip1"
                     /locus_tag="POH73_001107"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HDI6887073.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPRDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDLNANNNLPSQSRTKVSPSLNKSDQTSQREIVNETEIEKVQPQQKNQANDKITDH
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKGDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGSREEIEYQKKHSGEISPLFKGNNDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTNATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKVTDTKQA"
BASE COUNT         6928 a         2808 c         3636 g         5910 t
ORIGIN      
        1 tatcaacact gatacgatca tcaaatacat acattttacc gtcccaatat tgaccttttg
       61 tttcagggtc tttaccatat gtagctatac cgccgtgaag ctgcgctaca tcttcgaaac
      121 cttcttttaa taaccagcca gaaaattttt cacatcgaat gccaccagta cagtacgtaa
      181 ccactttttt atctgcaaat aacgctttat tctctttaat ccagtcaggc aaatctctaa
      241 agcgcgtgat atttggacga attgcacctc ggaaatgacc taaatcaaat tcataatcat
      301 tacgtgcatc aataatgact gtgtcatcat cttcaagagc ttttctaaat tctacaggtg
      361 ataaatattg gccagttgtg tgtcttggat cgacgtcttc ttctaaatct aaagcaacaa
      421 tctcttttcg aggacgtaca tgcattttct taaaagcatg tccttcagct tcatcaattt
      481 taaacaccat atctttgaat cgttcatcgg catgcatatg tgccatatat tgttcggttt
      541 cttcttttgt accagataat gtgccgttaa taccttctgt agaaacaaga attctacctt
      601 ttaaatggtg tgctttacaa aaggctaagt gatcctgagc aaactgttca gggtcatcaa
      661 tcgtcgtata tttataatat aaaagaactt gatagttcat aggttaatct ccttctaaaa
      721 acatgttata agcatgttaa tttttaaaat aaagcgtcaa tacgcatatt tgtctacgtc
      781 catcatttta acagagttgt attgtcattc aatatttcta gcttgaaatc agccactaga
      841 ccgataaaat agataaatga aaacgttatc atttgatttg atagttacct tgtttaaaat
      901 gaaaatgata gttaaaacga atgaaatgaa aggaatgatc atgatgtcac aacaagattt
      961 acctacatta ttttatagcg ggaagtctaa tagtgctgtt ccaattatat ctgaaagtga
     1021 attacaaaca attacagctg aaccatggct tgaaatttcc aagaaaggat tgcaactaga
     1081 aggattgaac tttgatcggc agggacaact ctttttattg gatgtattcg aaggaaatat
     1141 ttttaaagta aatccagaaa ctaaagaaat taagcggcct tttgtaagcc ataaagcaaa
     1201 tcctgcagca atcaaaatac ataaagatgg ccgattattt atttgttatt taggagattt
     1261 taaatctaca ggaggcattt ttgcagcgac agaaaatggt gacaacatac aagatatcat
     1321 tgaagatcta tcaacagaat attgtattga tgacatggta tttgattcta aaggtgggtt
     1381 ttattttaca gattttagag gatactctac caacccacta ggaggcgttt attatgttac
     1441 gccagacttt aaaacagtga cgccgattat tcaaaatatt agcgtagcaa atggtattgc
     1501 tttaagtaca gatgaaaaag tactatgggt aacagaaact acagccaatc gattacatcg
     1561 cattgcactt gaagatgatg gtgtgacgat acaaccattt ggagctacca taccgtacta
     1621 ttttacaggt catgaaggac cagactcatg ttgtatcgat agtgacgata atttatacgt
     1681 agcaatgtat ggtcaaggtc gagtgttagt ttttaataaa aggggttatc caataggaca
     1741 aatattgata ccaagccgag atgaagggca tatgttacgt tctacgcatc cgcaatttat
     1801 acctggcaca aatcaactca tcatttgttc caatgatata gaaatggacg gaggatctat
     1861 gctttatacg gttaatggct ttgcaaaagg gcataaaagt tatcagtttc aataagatct
     1921 gcctgaatga agggcgtctt aatataagtt taaatgttaa aatagtgaat cataaattaa
     1981 agaaaaatgc caactatacc tagggatgat atagttggca tttcgtatat attaaaatca
     2041 atgaggatta tgagatgagg ttattctgag attgagaatt cagctgatgc ttcgaattta
     2101 acatctttac ctagcattac tccaccagtt tcaagtgttt ggttaaagtt aatgccatat
     2161 ttttctctgt tgattatacc agtaacaata atacctgtta cttgagaacc atccatagga
     2221 tttttactta cgccgttaaa ctcaacgtcg aatgtttctt cgttagtgat acctttaatt
     2281 gttaaatcac ctacaacttt gctttctgta attgattttg tcacaaaagt gattttatca
     2341 aattcatcag taccaaagaa atcaccagat tttaagtggt tatcacgtgc ttcgttttta
     2401 gtgttaattg agcttggaat aattgttgca gtagctttta aagtgctgaa gtcattaata
     2461 tctccttcaa cagcgacatc aaattgatcg aatgaacctt tcactttaga aaccattaaa
     2521 tgtttaattt ggaactctaa actactgtgt gcaccatcaa aagtaaagtt tgtcataata
     2581 taaaacctcc atatttttat ctcttagata tattttttat acttgatata catacgatac
     2641 cacttgaaaa acaaagatgc aagcatttta tttcgaattc gaatattttt ttatgatgca
     2701 tataaaagac atatctataa aacaccacac tattgttata ccctagtcaa ttaattcaaa
     2761 cataaataag tgtaaccaac gttcaataaa tttagttaat gagataaatc tgactattat
     2821 tatcattttt atagtcgcta ctattgactt tgtaacaagt gactcgtact attttaatat
     2881 aattctgaaa attcaaaata tataacctta aatatgtaga taatattttt tgaagtttta
     2941 aaaaaaggag gcattttatg ggaaattatt tggtattgca atcagatttt ggacttagtg
     3001 atggcgctgt aagtgcaatg tatggagttg catatactgt aagtgatgat atacgtgtcg
     3061 aaaacttaac gcatgatata ccaccgtatg acatttgggt agcgtcatat cgtttatatc
     3121 aaactgtaaa atactggcct aaaggcacag tttttgtatc agtggtagac ccgggtgtag
     3181 gtagtgatag gcggagcatt gcttgcttaa catattcagg tcattacatt attacacctg
     3241 ataatggctc tttaagtcat atcaaacatt atgaaggtat taaaaaagtt atcgaaattg
     3301 atgaagtgaa aagccgattg cctcattcgg aagaaagtca tacttttcat ggtagagatg
     3361 tttatgcata caatggagcg agattagctg caaataagat agcgtttgaa cgtctcggtc
     3421 aggcaattaa tgttgatagt attgaggcat tagaaataag tgaagcgaca aaaaatgagg
     3481 attctgttac aggaagtatt gatattttag atatcagatt tgggtcatta tggacaaata
     3541 ttccattggc attcttaaaa tccttggaaa ttgttcatgg caataatttg gtggtaacaa
     3601 tttatcatca agataagaaa gtctatcaaa atattattaa atttgcgcga tcgtttgcag
     3661 atgttaatgt gggtgaacct cttgtgtatg ttaactcgct agtaaatatt ggtgtagcgg
     3721 tcaatcaaga ctcattttca gatttatatc atattggtac tggaaatgat tggacgattc
     3781 atttatctaa agcaccatcg atttaatgaa ttttttatgt gaggagtaat gaagatgaaa
     3841 aaacaagata tttcagttaa aactgttgtt gcaattggta taggagcagc ggtctttgta
     3901 attttagggc gttttgttgt aataccaaca gggtttccta atacgaatat agaaacatct
     3961 tatgcatttt tagcattaat atctgcaata tttggacctt ttgctggttt aatgactgga
     4021 ttaattggtc acgctattaa agatttcact acatacggta gtgcctggtg gagttgggtt
     4081 atttgctcgg gaattatagg ctgtttatat ggatggattg gcctaaaatt aaatctttct
     4141 tcaggacgat tttctaggaa gtcgatgatt tattttaata ccggtcaaat tattgcgaat
     4201 attatttgct gggcacttat tgcaccaaca ttagatattt tgatttataa cgaaccagct
     4261 aacaaggttt atacacaagg tgttatctct gcagtattaa atattatttc agttggcatt
     4321 attgggacaa tattattaaa agcatatgct tcatctcaaa taaaaaaagg tagtttacgt
     4381 aaagaataat tattttgttg aatcagatat gtaaatgaat gtagaaaggt aatgatatat
     4441 catgactgaa ccaattatct cgttcaaaga ctttagtttt caatatcata gtcaagcaac
     4501 acctacatta cagaatataa atgttgatat ttatccggga gaaaaagtat tagtagttgg
     4561 tgcttcgggt agtggtaaat cgacttttgc aaattgcata aatggattaa ttccatttaa
     4621 aactaaaggt aacgtaactg gggaactaca tataaataat caagatgcaa ctgttagttg
     4681 tttacataaa agatctaatg ttgttggtac agttttacaa gatacagatg gacagttcat
     4741 aggcttaaca gcagctgaag atatggcctt tttattagaa aataattgtg ttgaacaaga
     4801 tgatatgaag aaaaacgtga gttattgggc tgaaaaagtt gatatgatag aacatttaaa
     4861 tcaccgaccg caagatttat ctgggggtca aaaacaacgc gtttcattag gtggtatatt
     4921 aatccatcgt acccctattt taatattgga tgagccactg gccaatttag atcctgcgac
     4981 aggacatgaa acgctgagat tgttaaataa tattcatgaa gaaacaaagt caacgatgat
     5041 tattgtcgaa catcgattag aagaatcatt agatgatacg tttgatagag ttcttctttt
     5101 taaagatgga aaaatcatcg ctaatacaac gcctagtgat ttgctaaaat catctaagct
     5161 taaagaagca ggaatacgtg aaccattata ctgtacagcg cttaaatatg cagaagtaga
     5221 tgttgaatcc attgataact tagcgaattt acgtgaagtt tgtatgagtg aacatgttaa
     5281 atttaaagtg aaaaaatgga tagataaaac atcttcaaat gatgataata agtacaaatc
     5341 tgaaccgcta ttagaattaa atgaggtgtg tgttcaatat agtgactata gtaatagtgt
     5401 acttaataat gttcaattaa atgtttatag aagagaaatg cttagtatag ttggtcacaa
     5461 tggtgccggc aaatcaacac ttgctaaagc aatttgtgga tttttagata taacaggtaa
     5521 tatccaattt tgtaataagg gatttaatca attgtcaatt agcgaacgat ctgaattcat
     5581 aggttatgtg atgcaaaatc caaatcatat gatttccgaa aaaatgattt acgatgaagt
     5641 agcattaggg ttaagagcac gaggtatgaa agaatcagat ataaagatac gagtagagaa
     5701 tgtgctcaaa atatgcggac tttatgcatt tcggaattgg ccaattgcag ctttaagcta
     5761 tggtcaaaaa aagagggtca ctatagcatc tgttttagtc ttaaatccgg aaataatcat
     5821 attggatgaa ccgactgctg gtcaagattt ctatcattat aatgagataa tgtcattttt
     5881 aattgagcta aacagacagg ggaagacgat tattatgatt acgcatgata tgcatttatt
     5941 gtctgagtat agttcaagaa cagttgtatt atcaaaaggt caagtcgttg ctgataccac
     6001 gccagtattg gttttaaatg ataaaaaaat ctgtgagatt gcatcattga gacaaacatc
     6061 gctatttgaa atggccgaat atatagggat tagcgagcca cagaaattaa tacaattatt
     6121 tattaaccat gataggaagg tgagacgcca atgaatcaat ataatactat aggttttcac
     6181 ccgggaaata gtcgtattca tcaattaaat gcgactgtta aacttttatt cttattagtt
     6241 gtttctattt ctgcaatggt gacttatgac acaagatatt taattttaat tagtgtttca
     6301 tctattttat tgttcaaata tgctcatatt gaatggaaac aagttcgttt tgttgttaaa
     6361 tttattctgt ttttcacaat attaaatatt attgccgtat atatatttga tcctgaatat
     6421 ggtgtgaaga tttataatca gcgtacagag ttaatcaatg gtattggtcg atttacgcta
     6481 acatcacagg aattattcta tctgtttaat ctaatattaa aatatattag tacagttcct
     6541 ttagcgttaa tatttttatt cacaacgaat ccgagtcatt ttgctgcaag tttaaatcag
     6601 ctaggtgtga attataaaat cagttatgca gtatcactag cattaaggta tattccagat
     6661 attcaagaaa catattttaa tatttcacaa gcgcaacaag caagaggata tgataattcg
     6721 aaaaaggcga aatttactag ccgtgttaaa ggtatcaaac gcattgtgtt acctttaata
     6781 ttttctagta tcgaaagaat tgacactatt agtactgcta tggagttaag acaattcgga
     6841 cagtataaaa ggagaacctg gtacgtcaaa aaacaattaa aaaaagatga ttatgttgtt
     6901 ttgtgtttga cgttaatact tctgatgtta gtagttacat tattcttttt aaataatagt
     6961 cgatatttca acccgtggca ttagtattca tataaatagt ctttaaatag aaataggagg
     7021 gagacattta atgataaata ctgaaagatt aaatttaatg attccaagtt cctcgcattt
     7081 aattgatctt tataatattt gtagtcatcc acaagcaaat atatacactc ccaaaggttt
     7141 acataattcc aaattagaca cacaacggtg gattgaaaaa tggcaaaacc attggcaaca
     7201 atatcaattt ggttactttg tattggtgaa aaaaatagat tgtagtgtta ttggtatttg
     7261 tggatatgaa tatcgacaat taaagcaaga aacagtatta aatttatttt ataaattaca
     7321 tccaagtttt gaaggacaag ggtacgcatg tgaggctatt acagcaatca caaattttgt
     7381 gaattatatc gatcaagaaa cagtaaaagt tatcaggaca aataagtgta accaacgttc
     7441 aataaattta gcagaaagac ttaatttcaa gcgagacgat acaatggacg acattatcaa
     7501 tcaaggagat attgtgtttt ataaataaaa tactatgaca ttatctaaaa aataaaatta
     7561 aaatcccaac agtttcttaa aaagttatga gccacataat aaaaatgtta taattgtgca
     7621 taataaaaca tagggagaat gtttaatgca taaaaaatat ttattgctat tttttctgtt
     7681 attatcagtt gcattgttat atagctgttc caagcaaaat gagaaagctc aaaataaatc
     7741 tgttaatcca aatgagtatg caatgcataa atcgaaaatg gatgaacatt gggaaacata
     7801 tcacggccaa gtttatcatg tgttttatca tccattaatt acagacccaa aagttgcgtt
     7861 tacaggagat acacaccaag ctaagggcaa caatgattgg atgattactg taagtgaatt
     7921 taaaaaatca ttgaacgaat tatataaaaa taattacatc atcgttaatc cacatgatgt
     7981 atacgactta tcatctcatc atgtaaaagc caaaaaagta aaattgccga aaggtaaaaa
     8041 gccgctaatc atctcaattg atgatatgaa ttactatagt tacatgagac accatggtta
     8101 tgctgaccgc ttagtacttg ataaaaataa gcatgttgta tctgaaacga cagactcaaa
     8161 aggccatgct acttattctg atgacaatga tatcgtgcca attttaaata aatttgtaaa
     8221 agagcatcca gatttttctt taaatggtga aaaaggtgtt gttgctttaa ccggttatga
     8281 gggtgtatta ggatatcgca cgaatgaatt aacgagtaaa gattatacaa agaataaaaa
     8341 ggcagctgag gaagttgttc gcgcaatgaa acgggatgga tggtcatttg cgagtcattc
     8401 atatggtcac attaattttg aaaaaacatc tttagatggt attaaacgtg atactaaaag
     8461 atggaaagat gaagttgaac caattgtagg taaaacagat atgtttgtat ttccacatgg
     8521 cgcacaggat agacatacac aagcgtatga ttatttggtc gataaagcag gctttaaatt
     8581 tattgcaggt gtcggtccaa acaattttac agatatatca gcaacgaacg tttaccaaga
     8641 tagggttgcg attgatggtc taaacttatt tgaatttaaa tataaactca aaccgttttt
     8701 taatcctgaa aatgtatata gtaaacaaga tagacaattc tttaaaggta atcgggatta
     8761 tgaagaatag ttaaatttaa taaaaagata gaaagaaaat aactttacat aataaggcaa
     8821 ggacaatatg tgcaagaagt acaacttcaa tataaaaatc ttggttaagc atttttatag
     8881 gcacaaatgt cttgcctttt tcttttgaaa aaatttaaaa ccaatacacc gaattttctg
     8941 aaaaacgttg taatgtattg ctaaaaatga taagttatta agcatattac actttagtat
     9001 ggtagaggag taaagtgaac aacaaaaata ccaggtgatt ttaaatacag tattgaaagt
     9061 aaagggtggt cgagtgaata attcagaaca attaattgcg ttaaaagaat cggaaacagc
     9121 atttttaaaa tattttaata aagcagatta tgagttagtt gattttagtt tggttgaaaa
     9181 gttggactgg aaacaattga accatgagga tttgcagcag atgggtgagc gaaatttttg
     9241 gcaacatgag catcaaattt atgcacttcg aaatgatttt acagatcaat tattgcgtta
     9301 ttactcaatg tatccaactg cagcaacaaa agtggcatac acaggtttaa tcattcggaa
     9361 taatgaagcg gctgtacaag ttggtttgga aaactatgca ccatcattag cgaatgtaca
     9421 acagagctta aagctattta ttcagtttat tcaacagcag ttgagagaca acgtgcattt
     9481 tgttgtattg ggacattatc aattgcttga tgcgttactc gataaatcgt tacaaacacc
     9541 agacatacta tcaatgattg aggaacgtaa tttatcggga ttggttacat atttgtcgac
     9601 tgaacatcca atcgtgcaaa tcttgaaaga gaatacgcaa caacaattaa atgtattgga
     9661 acactatatt ccaaatgacc atccagcact cgttgaatta aaaatatggg aacgttggtt
     9721 acatacacaa ggttacaaag acatccattt agatattact gcgcagccac ctagatccta
     9781 ttacacaggt ttatttattc aatgtcattt tgctgaaaat gaatctcgag ttttaacagg
     9841 tggttattac aaaggaagca tcgaagggtt tggattagga ttaacacttt aagtaaggga
     9901 gtatgcacaa tgttaagaat cgccatagca aaagggcgtc taatggatag tttaattaac
     9961 tatttagatg caattgaatt tacgacatta tcagaaacat taaaaaatag agaacgccaa
    10021 ttattattaa gtgtagataa tatagaatgc attttagtaa aagggagtga cgtgccaatc
    10081 tatgtggaac aaggaattgc agatattggt atcgttggta gcgacatttt agatgagcgt
    10141 cattacaatg ttaataattt gttgaatatg ccttttggag catgtcattt tgcggttgca
    10201 gcgaaacctg aaacgaccaa ttatcgtaaa atcgcaacga gttatgttca tactgctgaa
    10261 acatatttta aatcaaaagg cattgatgtc gaattgatta aattgaatgg ttctgttgaa
    10321 ttagcttgtg tcgtcgatat ggtagacgga attgtcgaca tcgttcaaac aggtactacg
    10381 ctaaaagcga acggactggt tgaaaagcaa catattagtg atatcaatgc aaggttaata
    10441 actaataaag cagcttattt taaaaaatca caattaatag agcaatttat tcgctctttg
    10501 gaggtgtcta ttgccaatgc ttaatgcaca acaattttta aatcaatttt cattagaagc
    10561 accattagat gagtcattgt atccaattat tcgcgatatt tgtcaggaag ttaaagttca
    10621 tggagataag gctttgaaaa tgtataatct aacattcgat catacgaaaa cagatcattt
    10681 agaaattagt catgaacaaa ttaaagcagc atttgacaca ttagatgaaa aaacaaaaca
    10741 agcattacaa caaagttatg aaagaattaa agcatatcaa gaaagtatta agcagatgaa
    10801 tcaacagtta gaagaatcag tggagtgcta tgaaatatac catccactag aaagtgtggg
    10861 tatttatgtg cctggtggca aagcaagtta tccatcaacg gttctaatga cagcgacttt
    10921 agcacaagta gcgggtgtag aaaatattgt tgttgtgaca ccacctcaac ctaacggagt
    10981 atcccaagag gtattagctg catgttatat tacgcaagtt aatcaagtgt ttcaagttgg
    11041 tggtgctcaa agtattgctg cattgactta tggaacagaa acgataccta aagttgataa
    11101 gattgtaggt ccaggtaacc aatttgttgc atatgccaaa aaatatttat ttggacaggt
    11161 aggtattgat caaattgcag gaccaacaga aatagcactg attattgatg acaccgcaga
    11221 tttagatgcc atcgtatatg atgtatttgc gcaagcagaa catgatgaat tagcacgtac
    11281 atatgtcatt agtgaagatg cgcaagtcct taaagattta gaatcacgta ttgctaaagc
    11341 attgcctaat gtggacagat acgacattgt ttctaaaagt atcgctaatc aacactacct
    11401 tatccatgct agtaattttg atgaagcatg ccacgttatg aatacaatcg cgcctgaaca
    11461 tgcgtcgatt caaacagtaa atcctcaacc atatattgaa aaagtgaaat atgtgggcgc
    11521 attgtttatt ggacattatt cgccagaggt cataggtgat tatgtcgctg gacctagtca
    11581 tgtattacct acaaatagaa cagctagatt taccaatggg ttatcggtca atgatttctt
    11641 aacacggaac acggtcattc atttatcaaa agatacattt gatcaaattg ctgattcagc
    11701 acaacatatt gctcatgttg aagcattata caatcaccaa cagtctattt taatacgtca
    11761 gtcttagggg agtgtaaatg aaatgattta tattgataaa aatgaaagtc cagttacgcc
    11821 gttggatcaa aaaacaatga cgtctattat tagtgcaacg ccatataatt tatatcctga
    11881 tgcagcatat gaacaattca aggaagctta tgctaagttt tacggattat cgcctgaaca
    11941 aattattgca ggaaatggct ctgatgaatt gattcaaaag ttaatgctga tcatgccaga
    12001 aggtccggta ttaacgctaa atccagattt ttttatgtat caagcatatg cggcacaagt
    12061 aaatcgtgaa attgcatttg tagatgcaag atcagattta acgtttgatt tggaaaccat
    12121 tttaacgaaa atcgatgaag tacaaccatc attttttatt atgagtaatc cacataaccc
    12181 ttcaggcaag caatttgata cggcattttt aacagctatt gcagataaga tgaaagcaat
    12241 aaatggatac tttgtcattg atgaagcata tttagattat ggcaaggcat atgatgttga
    12301 aatggcacca cacattttaa gaatgcgcac attatcaaag gcgtttggaa ttgccggctt
    12361 aaggttgggt gtattaatga gtactgttga aacgatacag cgtattcaaa caatagaaca
    12421 tccatatcca ttaaatgtat ttacgctaaa tattgcgact tatattttta gacatagaga
    12481 agagacaagt cgatttttaa caatgcaacg acagttagct gagcagttaa aacaaatatt
    12541 tgatacacat gttgcagata aaatgtcagt attcccatca aatgctaatt ttgtacttac
    12601 taaaggttca gcggcgcaac aattaggaca atacgtatat gaacaaggat ttaaacctcg
    12661 tttttatgat gagccggtga tgaagggcta tgtaagatat tcaattgcaa cagcatcaca
    12721 gttaaagcaa ttagaagaaa ttgttaaaga atggagtgca aaatatgatt tatcaaaaac
    12781 aacgaaacac agctgaaaca caactaaata tttcaatatc agatgaccag tcaccatcgc
    12841 atattaatac aggtgtgggc tttttaaatc atatgttaac cttgtttacg tttcatagtg
    12901 gactatcgtt aaacatcgag gcacaaggtg atatcgatgt agatgatcac catgtaactg
    12961 aagatatcgg cattgttatt ggccaattgt tacttgaaat gattaaagat aaaaagcatt
    13021 tcgttcgtta tggaacgatg tacatttcaa tggatgaaac attagcacgt gtcgttgtgg
    13081 atataagtgg gcgcccatac ttatctttca atgcaacgtt aagtaaagaa aaagttggta
    13141 cgtttgatac ggagttagta gaagaatttt ttagagcagt cgtaatcaat gcaagattaa
    13201 caacgcatat tgatttaatt cgtggaggta atacacacca tgaaattgaa gctatattca
    13261 aagcattttc ccgtgcatta ggcatagcgc taactgcaac tgatgatcag cgtgtgccgt
    13321 catcgaaagg tgtgattgaa tgattgtcat cgttgattat ggattaggga atattagtaa
    13381 tgtaaaacgt gctattgaac atttggggta tgaggtggtt gtctcaaata agcaaaatat
    13441 aatcgatcaa gcagaaacaa tcatattacc gggtgtcggt cattttaaag atgcgatgtc
    13501 agagataaag cgattaaatc tcgatgcaat attggctaag aatactgata agaaaatgat
    13561 cggtatttgt ttaggcatgc aattaatgta tgagcatagt gatgaaggcg atgcatctgg
    13621 attagggttt atcccaggaa atatttcgcg tatccaaaca gaatacccag tgccgcactt
    13681 aggttggaat aatttagtga gtaagcatcc tatgttaaat caagatgttt acttcgtaca
    13741 ttcttaccaa gcgccgatgt cagaaaatgt cattgcatat gcgcagtatg ggactgatat
    13801 tccagcaatt gttcaattta acaattatat tggtattcaa ttccatcctg aaaaaagcgg
    13861 tacatatggg ttacaaattt tgcgtcaggc aatacaaggg ggatttataa atgattgaat
    13921 tatggccagc gattgatttg attgggtcaa caagtgtgag gttaacagag ggtaaatatg
    13981 atagtgaaga aaaaatgtcg cgttcggctg aagaaagtat tgcttactat agtcaatttg
    14041 aatgtgtgaa tcgtattcat atcgtcgact tgataggtgc taaggcacag catgcccgag
    14101 agtttgatta tattaagtca ttgaggagat taacaaccaa agatattgaa gtaggtggtg
    14161 gcattcgcac gaagtcacaa atcatggact actttgccgc aggaattaat tattgcatag
    14221 ttggaacgaa aggtattcaa gatacagaat ggcttaaaga gatggcacat acatttccag
    14281 gtcgcattta tttatctgtt gatgcttatg gagaagatat taaagtgaac ggatgggaag
    14341 aggacacaga gttgaattta tttagttttg tgaaacagtt atcggatata cctattggag
    14401 gcattattta tactgatatc gctaaagatg gcaaaatgtc cggacctaac tttgaattaa
    14461 ctggtcaatt agtaaaggca acgacgattc ctgtcatcgc gtctggtggt attagacatc
    14521 agcaagatat tcaacgatta gcgtcgctaa atgttcacgc tgctattata ggaaaggctg
    14581 cacatcaagc atcattttgg gaggggctaa aatgattaaa aaacgtatca ttccatgttt
    14641 agatgtcaaa gatggtcgtg tcgttaaagg gattcaattt aaaggattaa gggatattgg
    14701 gaatcctgtt gatttagcaa tgtattacaa tgaagcgggt gctgatgagt tagtattttt
    14761 agacatctct aagacggaag agggtcatag cttaatgcta gaagtgattg aacagacagc
    14821 gtcacgcttg tttatccctc ttactgtagg gggtgggatt caaagccttg atgatattac
    14881 ccaattgtta aatcatggtg cagataaagt atcattaaat tcaagtgctt taaaaaatcc
    14941 acagctaatt aaacaagcga gtgataaatt cggtagacaa tgcatctgca tagcaattga
    15001 tagctattat gatcctgaaa gaaaagcaca ttattgttgt acgcatggcg gtaaaaaatt
    15061 gacaaatatt aaagtatatg actgggtaca gcaagtagaa cagttaggtg caggtgagct
    15121 cctcgttaca agtatggggc atgatggtat gaaacaaggc tttgatattg agcatcttgc
    15181 aaaaataaaa tcccttgtaa atattccaat cattgcttct ggtggtggtg gcaatgcaca
    15241 acactttgta gaattatttg atcagacgga tgtttctgca ggtttagctg caagtatatt
    15301 acatgaccga gaaacgacgg ttcaatctat taaagaagcg atacggcaag ggggtatagc
    15361 agtaagatga ccaattacaa aattgatttt agcaaaggtt tagtgccagc aattttacaa
    15421 gataatcaaa caaaacaagt attgatgtta ggttatatga atcaagaagc ttttgataaa
    15481 acgatagaag atggtgtggt atgtttctat tcgcggtcga aacaacgcct atggacaaaa
    15541 ggtgaaacat ctggtcatac gcaacgtgtt aaagatattc atgtagattg cgataatgac
    15601 actattttaa ttgatgtcat accaaatgga ccaacatgtc atacaggcag tcaaagttgt
    15661 ttcaacacag aagttccatt ttcagtacaa acattagcgc agacagttca agatagtgcc
    15721 caatccaata atgaaaagtc atatacgaaa tatttattaa cagaaggtat agaaaagatt
    15781 acaaaaaaat acggtgaaga agcttttgaa gtcgtaatcg aagcaattaa aggtgacaaa
    15841 aaagcatttg taagtgaagt agcagatgaa ctttatcatt tatttgtctt gatgcatgcg
    15901 ctaggcgttg atttttcaga aattgaggca gaattagcgc gtagacatca taagcgcaat
    15961 aactttaaag gcgaacgaca aaatatcgaa cagtggtaaa gcaagtatgg actgagatat
    16021 agagaaaagg atcatggctt atacacttac aaatattgtg ggaaacgtga cattttcaag
    16081 tttaaaatac gacaccaaca tattttaact atgaatgctg tgatggtact aaagttgcga
    16141 actcgttata gataactagt ggataatcac gatacgaaac caaagataat ttgaaaagtg
    16201 aattgagcaa ctcaggaata gatgtcactg ttaaagatgt tgaaaagtat atgaatcgat
    16261 ataatgaagt tatgaaggga aaaatggcga aaaagctaaa aagttatgtt tgtcgttact
    16321 acctattaat atcatagttg tctttatatt ctttgtattt atactgtgaa taaaaatata
    16381 tctagcctga aatagaaatg tcatagccta tttaaaagac aatctccatt agaactaaga
    16441 tgtgcatccc gaaagttgga ctaaaaaact aactttatgg ggtgtatttt tatgctaatc
    16501 atcataaatt cgagattaag ttaaaggtag ttcaatagta attaaacaat aaattaaaaa
    16561 tagtaggata cttactttga gggaagaaaa ttaactgtat atatttaatg taggaacaag
    16621 cgtaatggct tatcctaata caatcattgt agaagaaatg atatttttag gtttaaaata
    16681 caacaccagc aaacattata actgtgatag ctcataaatc tccccataca gctaatctaa
    16741 aaaaataata catcattgga atcaagaccc aagcatgtaa atattaaaaa tcacaaaaga
    16801 tatatgtaaa aaagttacaa tttgcataat taaattgtgt ctaattattg actaattaaa
    16861 ttttgccaaa tataatatta attaataatt tgaaatggtt agcgtataca ctttaaattc
    16921 tctttggaga atatattttt taaataaaaa tgtaaacgct ttctcatcaa attaaacaat
    16981 agaaaggatg gtcattatga gtgcttggtt aagtaaatta tttgagttta ttcctcgaat
    17041 aattatcaat ttgtttatct aaaataaaaa aatagaggtg ctgacaatga tgaaaagtca
    17101 aaataagtat agtattcgta aatttagtgt aggtgcatct tccattttaa tagctacatt
    17161 actattttta agtggtggac aagcacaagc agctgagaag caagtgaata tgggaaattc
    17221 acaggaggat acagttacag cacaatctat tggggatcaa caaactaggg aaaatgctaa
    17281 ttatcaacgt gaaaacggtg ttgacgaaca gcaacatact gaaaatttaa ctaagaactt
    17341 gcataatgat aaaacaatat cagaagaaaa tcatcgtaaa acagatgatt tgaataaaga
    17401 tcaactaaag gatgataaaa aatcatcgct taataataaa aatattcaac gtgatacaac
    17461 aaaaaataac aatgctaatc ctagggatgt aaatcaaggg ttagaacagg ctattaatga
    17521 tggcaaacaa agtaaagtgg cgtcacagca acagtcaaaa gaggcagata atagtcaaga
    17581 cttaaacgct aataacaatc taccttcaca aagtcgaaca aaggtatcac catcattaaa
    17641 taagtcagat caaacaagtc aacgagaaat tgttaatgag acagaaatag agaaagtaca
    17701 accgcaacaa aagaatcaag cgaatgataa aattactgac cacaatttta acaatgaaca
    17761 agaagtgaaa cctcaaaaag acgaaaaaac actatcagtt tcagatttaa aaaacaatca
    17821 aaaatcacca gttgaaccaa caaaggacaa tgacaagaaa aatggattaa atttattaaa
    17881 aagtagtgca gtagcaacgt taccaaacaa agggacaaag gaacttactg caaaagcgaa
    17941 aggtgatcaa acgaataaag ttgccaaaca agggcagtat aaaaatcaag atcctatcgt
    18001 tttagtgcat ggtttcaatg ggtttacaga tgatattaat ccttcagtgt tagctcatta
    18061 ttggggcggt aataaaatga acattcgcca agatttagaa gaaaatggtt acaaagctta
    18121 tgaagcaagt ataagtgctt ttggaagtaa ctatgaccgc gcagttgaac tttattatta
    18181 tatcaaaggc ggtcgtgtag attatggtgc agcacatgca gcaaaatatg gacatgaacg
    18241 ttatggaaaa acatacgaag gaatttacaa agactggaaa ccaggacaga aggtacacct
    18301 tgttggacat agtatgggtg gtcaaacgat acgtcaacta gaagaattac tgcgtaatgg
    18361 tagtcgtgaa gaaatagagt atcaaaagaa acatagtggc gaaatttctc cactattcaa
    18421 aggtaataat gacaatatga tttcatcaat tactacttta ggaacgccac ataatggaac
    18481 gcatgcttca gatttagctg gtaatgaagc tttagtgaga caaattgtat ttgatatcgg
    18541 taaaatgttt ggtaataaaa attcaagagt agacttcggg ttggctcaat ggggtctaaa
    18601 acagaagcca aatgaatcat atattgatta tgtcaaacgc gttaaacaat ctaatttatg
    18661 gaaatcaaaa gataatggat tttacgatct gacgcgtgag ggtgcaacag atttaaatcg
    18721 taaaacgtcg ttgaacccta acattgtgta taaaacatac actggtgaag caacgcacaa
    18781 agcattaaat agcgatagac aaaaagcaga cttaaatatg tttttcccat ttgtgattac
    18841 tggtaactta atcggtaaag ctactgaaaa agaatggcga gaaaacgatg gtttagtatc
    18901 cgttatttct tctcaacatc catttaatca agcttataca aatgcgacgg ataaaattca
    18961 aaaaggcatt tggcaagtaa cgcctacaaa acatgattgg gatcatgttg attttgtcgg
    19021 acaagatagt tctgatacag tgcgcacaag agaagaatta caagattttt ggcatcattt
    19081 agcagacgat ttagtgaaaa ctgaaaaggt gactgatact aagcaagcat aatttataaa
    19141 gtaaagggag gaattaataa tgactgcaga cttctttcaa ttaatcggat cattatttag
    19201 aattctaaaa gagttattca agtaatacct tggcgggacc ccaacaaaga gaaattggat
    19261 tcccaaattc tacaggcaat gc
//
LOCUS       DAMZPP010000041        19106 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMZPP010000041 DAMZPP010000000
VERSION     DAMZPP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19106
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.041"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..334)
                     /locus_tag="POH73_001108"
     CDS             complement(<1..334)
                     /locus_tag="POH73_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6887074.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKQQKLQGYFV
                     FDKGMTKAF"
     gene            complement(402..1925)
                     /locus_tag="POH73_001109"
     CDS             complement(402..1925)
                     /locus_tag="POH73_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887075.1"
                     /translation="MSKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFSNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGTTKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYSDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            complement(2254..3147)
                     /locus_tag="POH73_001110"
     CDS             complement(2254..3147)
                     /locus_tag="POH73_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6887076.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKSNAPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWEGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
     gene            3395..3688
                     /gene="esxA"
                     /locus_tag="POH73_001111"
     CDS             3395..3688
                     /gene="esxA"
                     /locus_tag="POH73_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HDI6887077.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            3771..6800
                     /gene="esaA"
                     /locus_tag="POH73_001112"
     CDS             3771..6800
                     /gene="esaA"
                     /locus_tag="POH73_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HDI6887078.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDSD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKTIADSVSGQLNQL
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNNLWNNAITSGVIGATGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLALVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            6800..7258
                     /gene="essA"
                     /locus_tag="POH73_001113"
     CDS             6800..7258
                     /gene="essA"
                     /locus_tag="POH73_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HDI6887079.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIVKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            7230..7472
                     /gene="esaB"
                     /locus_tag="POH73_001114"
     CDS             7230..7472
                     /gene="esaB"
                     /locus_tag="POH73_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HDI6887080.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            7485..8819
                     /gene="essB"
                     /locus_tag="POH73_001115"
     CDS             7485..8819
                     /gene="essB"
                     /locus_tag="POH73_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HDI6887081.1"
                     /translation="MVKNHDPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKEDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            8841..13289
                     /gene="essC"
                     /locus_tag="POH73_001116"
     CDS             8841..13289
                     /gene="essC"
                     /locus_tag="POH73_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HDI6887082.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGSNAYD
                     DMVIQSLMNAIIIKDFQSIQETQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGVATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRKIHDIISERK
                     RLLSQERVVNIEQYNKETGNSIPNVFLIIDNYDTVKESPFMEEYEEMMSKVTREGLAL
                     GVYIILSGSRSSAIKSAIFTNIKTRVALYLFENNELTNIIGSYKKGVKDVKGRAAIND
                     DNFTQFQIAQPFELAEGQTYNERIKNEVAQMKEFYVGDYPKHIPMMPDKVFMEDIREA
                     YDLEKIIHEEHKLPLGLDFEDVELVSLDLTSSSIVTAIKPTEMEKMNDVIMSSLSVYS
                     KNQFVILVDAEDNMSQYSEDVTSYYSAPSDLSNIRLGFKQEIEARKNGEKSIEECKIV
                     FINNIKRFNQLTGMTEDEIRVLFNEGQKVNIIIIASGLYSDTIGAFDRESKMMVRTIN
                     QALISHKISEQEFIRVKDRFGEPELKVGEMYYINNQEYQKIKLMEG"
     gene            13292..13558
                     /locus_tag="POH73_001117"
     CDS             13292..13558
                     /locus_tag="POH73_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwqI/YxiC family protein"
                     /protein_id="HDI6887083.1"
                     /translation="MIKLNQASVSKEISSIRTNGQGLKQSNGNVNLSKTNLVTFKEYV
                     NMFEDYQSALSNYENIIEQDTTAMDTTVTEIVENDREIAGQINK"
     gene            13581..13976
                     /locus_tag="POH73_001118"
     CDS             13581..13976
                     /locus_tag="POH73_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5082 family protein"
                     /protein_id="HDI6887084.1"
                     /translation="MSNKGEIRRQIANKEREKASKEAQLTDLKEDLRRLKDASKKLDT
                     AGEDFNKGQSSYNKVEISTSDWKGERRTKSDSKKKDVDSELKKVEQDFDDAKKAIKKD
                     IQDKEEEIKGVEGEISTINAAIDALKSKL"
     gene            13998..15668
                     /locus_tag="POH73_001119"
     CDS             13998..15668
                     /locus_tag="POH73_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000540847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6887085.1"
                     /translation="MGYKVDMSEVHNMQKSIDSSLSAINTKVSTLSSSINNLINTEGF
                     EGQTASSVKNYSKTFHLQSIKKIETINKDFKSDIAKSIRKFQSEVDNNASAILDEDEI
                     KKYKKDIDDALKDVFKSSKDANGAISDVSDLTTAKKIKTENLANKMGDFNKDIDQTVE
                     RLTTFDANNSIDGDKTDNLITELSGVNSYVKNMKPNRARISSTSGKIEGAIARHKTSE
                     ELVRWQTYMETLSENLYKTPGLSKAASDIITQAGREYYAIKAVGNGSALRGYKEYLKT
                     RDINKLINKMNKNQLKRMSLVLNTDRGNIKIKNAVKMASEFARNNPFKKGNLVNWMSK
                     VQGYQSVSAQILKKGLQDKNFKYTFGDAKKFFDTADMKKAAVTEFKNTFVPQNIRETF
                     FKKENLTSKTALKKNMSKFWNEDIKGGIKEFKLDFANKNIIGKLGKLLKLGGKALKPL
                     GFITAITDNIGKKSWQEKLVGMGVDLSAIGASAAAGAAVGTAIPLPVVGTVAGALAGA
                     AVGIALDYKIPYLDKSATELAKDGINSGINKVKSGFGKVTSGFKTVFG"
     gene            15684..16328
                     /locus_tag="POH73_001120"
     CDS             15684..16328
                     /locus_tag="POH73_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDI6887086.1"
                     /translation="MKIQEIVGNIKRAYLTPLIIFVILFSLFFDLIMYFYGNLQGKLP
                     IYLDVFLIFAAVVFILMYFQEKSGEVKVEKSNVIRYLTLNVVAGYSMVLLVASIYVFG
                     VAGYGFDVFNYWLGIILMLFVSWFALFLFYKNEFDSENPNKTVNVIAIIIKLSAFGGL
                     FYIRTVVPNTADEEKFITLSILINIAVDLLLVRSYFNYALYKSVKKDIENGVDD"
     gene            16328..16783
                     /locus_tag="POH73_001121"
     CDS             16328..16783
                     /locus_tag="POH73_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5085 family protein"
                     /protein_id="HDI6887087.1"
                     /translation="MEVGMLIMPFCVKITFEIDKNDNWLNALDTVNEFFMDKDIYPTG
                     PIIFQRELAGLGEYKYTAYIALNDELQDIPELNIKYIDCLEVGPTLSTKCFEEEEFER
                     AYKEIEYVANENNIKILNQPYYHVMVDYFGGTAFEIYAQVDLDESEVYG"
     gene            16776..17234
                     /locus_tag="POH73_001122"
     CDS             16776..17234
                     /locus_tag="POH73_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5085 family protein"
                     /protein_id="HDI6887088.1"
                     /translation="MDKNGYSYIMMKNVAYKTYFDVTMDDYEDILKDFTNLAISKGKT
                     ITGPLTFAVTQIDLQKRMNIDLFISVGKCFKSNEELSYRTYFCLDNMLHGRITSNNFI
                     TDEIALLEDMNEFASDNNLTFTSPYYHTMRKSFSGEQGWIDVKAKVYEND"
     gene            17258..17845
                     /locus_tag="POH73_001123"
     CDS             17258..17845
                     /locus_tag="POH73_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HDI6887089.1"
                     /translation="MIIFILLTVFALFYIAMIASLFKSEGFSIIGLILDIVILTTLIF
                     YYFVGASFVDNDLSNFLAFMNFGSFVYMYYAIKSLWVKPKLVNYIIAKEIGESKDVIE
                     EQELDLQTSKIRGIYFFIIAIALLIITKLRMQPELQADAISMNPVFIFIGVIIILIWL
                     VLDIYRKKKYGIFLFKTIVPLVVTTWIIIATIVLS"
     gene            18022..18240
                     /locus_tag="POH73_001124"
     CDS             18022..18240
                     /locus_tag="POH73_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887090.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLKIAIIVTIIVFVLNLLAYLGLR"
     gene            18372..18872
                     /locus_tag="POH73_001125"
     CDS             18372..18872
                     /locus_tag="POH73_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDI6887091.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPIEWEKVYTMAYIDDEGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSAYELYKQFQNLRNIFKEEGYEPWTSCEF
                     DFTKEGELKVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKEIEQYIKE
                     QEEAEQ"
     gene            18883..>19106
                     /locus_tag="POH73_001126"
     CDS             18883..>19106
                     /locus_tag="POH73_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDI6887092.1"
                     /translation="MTFEEKLSEMYNEIANKISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKINSDELNYYTDIPKEYNISVQVFDDL"
BASE COUNT         7201 a         2517 c         3406 g         5982 t
ORIGIN      
        1 aaaatgcctt ggtcatacct ttatcaaaaa caaaatagcc ttgaagtttc tgctgtttta
       61 gcaaagtatg tgcttgctta tcatcatatg ctttaatgct cacattttta cctaggttac
      121 tccctttacc aatagagttt aagattaatt tcgtttcact tgattggtct ttatctacga
      181 cacctatatt aaaatgattg tcatcttctg ttacatgttg gattgtcgtt aatgtaataa
      241 ggagtgccgc taatataaat agtaaataga taatcaaata ccactttttc aataaaaaag
      301 agtggtagat gcgaaacaaa tgtattgttt tcatttaacc actcctccaa tgataagatt
      361 gaaaggcaag atgaccttcc aatcttattt ttatatttta gttatttaga tgcctttttt
      421 aaaattgatt caaacatttt gccgccattt ttttcgattt ctttttcaag tttttcacgg
      481 tcttttgaag atagactatt gaaatctttc gcaccactat catcaaaatc aatatctgct
      541 ttcaattttg tgctagattt taaaatgaaa ttaattggtt cttcagcata tttgatgccg
      601 atatttaacg tagatttctg agtgttattt tttacgtcag aatctatatt attttcaaaa
      661 gtgaattcat tttcgtcgct atatttatct aacgcgacag tgattttacc tttatcttga
      721 cgttttgtgc catctacttt ttcttggtta tctaatttga tttttgattc atcatattct
      781 gtctttttac caaattcgta tttatcactg tatttattgt ctttttcctt agaagatacg
      841 cctttaattg tatatttcgc ttcagcatac gtgtatttat cttgatcgaa atcaagtgcg
      901 taatctagtt ttaacttatc gtcttctaaa gtattagtac ctttgatttt agttttatta
      961 ttttctttgt ctgtaatagt aatttctcgt tttacaatcg tatgtttttc ggtataaatt
     1021 ttagattgaa ttttagcaaa ttcatccttt ttagtttctt tgacatcgtc aattgctttt
     1081 ttaatgtctt tttcaaagtc ttttgtagta ccttgttctt ccattaattt tttaaggtct
     1141 ttatcctttt tagcttcttc taatacagct aatgtaattt ttttagtgtc agctctgcta
     1201 agtgttaacg tgacaggtct aactttgtac ttttcaccat taaccttaat ttcttctttt
     1261 ttacctttat caaaattatc gtcatctaat ttgtcgacaa taagttcgga atatttttcg
     1321 gcaattttgc tgtagtcact ttgttgtgct tgagcattac tgaaaagagt atttaaattt
     1381 agttgttggt ttgtaatacc attttctttt gctgtttctt catcttcacc tgtaagtttt
     1441 gaataagttg ataataaatc agaattatta acactatatt tccctttaaa taatggtgat
     1501 tcgaaataat gcttatcttt atctgcagct aactggaatt tccctaatgc agagtctgct
     1561 attgttggtt caagattaat cattgatttc tcttttttag gatcatgtcc atatgacatt
     1621 ttaattttcg aagcattaac aacagattta ggaataccaa gccctttaac aatttcatca
     1681 gatgcatctg cgcttaattc taatgaagat aaaaatgaat tatctttcat cttttcttgg
     1741 aacttcactt cgttttcaaa acggtcatta aaataatctt tatacatttt tgcagtttgt
     1801 tgttcacttt ttaagtatgt atttttcggt gtgtttgcaa aaaatgcata aactccccat
     1861 gcgattccac ctattaataa taagacaata ataataggaa ttataatttt taacttttta
     1921 gacattttgc ttcctcccaa ttatttatga gttaatcata tcagaacaat acattttatt
     1981 aaatggttta ttttaaaatt tgtttaaatt aatagaattt tagttataat aatgtttaat
     2041 aagttattga aaatctaata aactacaaaa atagtttgat tacataatga ttcttgaaaa
     2101 tgtcggttaa ctttaaggtg acaagttatt gcaattttga aggtagacat catattttat
     2161 aatcgaataa catacaaaat aaagcgacca attttgcagt gttacgaatt gtaaactgcg
     2221 aactggtcgc tttttattga tgtcatgatt aatttaatgg atgtaattat atgatgaaac
     2281 ttctgaagca gagatggttc ttgatgaaac gatatattca ccaatccagt tcatttctga
     2341 aattagaata cttccatcaa tattaacttt ctcaacgtag gctacatggc caaatggacc
     2401 gtttactgtt tgtaaaattg atcctcgtgt tgggtgtcta tctactttga agccattgct
     2461 tgaagcttgg ccttcccagt ttttagcatc tccccaaaat gtactaatcg tgtgtccatc
     2521 tttggcacgt ttatcaaaga cataccatgt acattgtcca gcagtatata agttgttctt
     2581 acttgtgata agaggctgat caatgatttt accgttacct aaagctaaag gtttaccgtc
     2641 agcagtcttt gctttgtcat taaattcggc gatttgtaat tcgtcataca attcgtctaa
     2701 atcgatgcct gtaataagcc ctttgttatc ttttgaaaaa gcgttattta attcatcgtc
     2761 attgtcttca acattcggta ttgctggtgt caaaggattg cttggtgacg tttgaggcgg
     2821 tgtgtgtgaa tcaattgcgt cattaatgtg cgtatactga ccacttaatg aagaatggta
     2881 ctgattgttg ttaagatcac gttgatttgc gtgttgatga ttgtcgtttg tacgtgactg
     2941 attttgatga ttgttgtttg gcgcgttgct tttgtcataa gtataagtat acgcgccggt
     3001 gtctttattc actttgaact gtgcgtttgg gtgtgctttc tttgcttctt ctaatgtttt
     3061 gctatcattc gtgtatgctt gagccgagtt aggcgacata ctaaataaag taagagttgt
     3121 catcgtcagt aaaattgttt tcttcataat aaccatttaa tcctttatgt atttaattta
     3181 attttagtat acacatttat attacaaaaa tgaatggtta attaaaaata tatgggtata
     3241 ttcaatatat ttatttaaaa aaagctaaaa atacttaaaa aactatatac atatactaat
     3301 aatttatata ttatttgagt aaggatcact ttttcaaaaa atagtgtccc taaaaagttt
     3361 tgataaactt aaaatattca ggaggtttct agttatggca atgattaaga tgagtccaga
     3421 ggaaatcaga gcaaaatcgc aatcttacgg gcaaggttca gaccaaatcc gtcaaatttt
     3481 atctgattta acacgtgcac aaggtgaaat tgcagcgaac tgggaaggtc aagctttcag
     3541 ccgtttcgaa gagcaattcc aacaacttag tcctaaagta gaaaaatttg cacaattatt
     3601 agaagaaatt aaacaacaat tgaatagcac tgctgatgcc gttcaagagc aagaccaaca
     3661 actttctaat aatttcggtt tgcaataagc attctgaaat tggcaaagtc acattttcta
     3721 atgtggcttt gcttatcatt ttttaagaaa acaactgaaa ggaaataagc atgaaaaaga
     3781 aaaattggat ttatgcatta attgtcactt taattattat aattgccata gttagtatga
     3841 tattttttgt tcaaacaaaa tatggagatc aatcagaaaa aggatcccaa agtgtaagta
     3901 ataaaaataa taaaatacat atcgcaattg ttaacgagga tcaaccaacg acatataacg
     3961 gtaaaaaggt tgagctgggt caagcattta ttaaaaggtt agcaaatgag aaaaactata
     4021 aatttgaaac agtaacaaga aacgttgctg agtctggttt gaaaaatggc ggataccaag
     4081 tcatgattgt tatcccagaa aacttttcaa aattggcaat gcaattagac gctaaaacac
     4141 catcgaaaat atcactacag tataaaacag ctgtaggaca aaaagaagaa gtagctaaaa
     4201 acacagaaaa agttgtaagt aatgtactta acgactttaa caaaaacttg gtcgaaattt
     4261 atttaacaag catcattgat aatttacata atgcacaaaa aaatgttggc gctattatga
     4321 cgcgtgaaca tggtgtgaat agtaaattct cgaattactt attaaatcca attaacgact
     4381 tcccggaatt atttacagat acgcttgtaa attcgatttc tgcaaacaaa gatattacaa
     4441 aatggttcca aacatacaat aaatcattac tgagtgcgaa ttcagataca ttcagagtga
     4501 acacagatta taatgtttcg actttaattg aaaaacaaaa ttcattattt gacgaacaca
     4561 atacagcgat ggataaaatg ttacaagatt ataaatcgca aaaagatagc gtggaacttg
     4621 ataactatat caatgcatta aaacagatgg acagccaaat tgatcaacaa tcaagtatgc
     4681 aagatacagg taaagaagaa tataaacaaa ctgttaaaga aaacttagat aaattaagag
     4741 aaatcattca atcacaagag tcaccatttt caaaaggtat gattgaagac tatcgtaagc
     4801 aattaacaga atcactccaa gatgagcttg caaacaacaa agacttacaa gatgcgctaa
     4861 atagcattaa aatgaacaat gctcaattcg ctgaaaactt agagaaacaa cttcatgatg
     4921 atattgtcaa agaacctgat tcagatacaa catttatcta taacatgtct aaacaagact
     4981 ttatagctgc aggtttaaat gaggatgaag ctaataaata cgaagcaatt gtcaaagaag
     5041 caaaacgtta taaaaacgaa tataatttga aaaaaccgtt agcagaacac attaatttaa
     5101 cagattacga taaccaagtt gcgcaagaca caagtagttt gattaatgat ggtgtgaaag
     5161 tgcaacgtac tgaaacgatt aaaagtaatg atattaatca attaactgtt gcaacagatc
     5221 ctcattttaa ttttgaaggc gacattaaaa ttaatggtaa aaaatatgac attaaggatc
     5281 aaagtgttca actcgataca tctaacaagg aatataaagt tgaagtcaat ggcgttgcta
     5341 aattgaaaaa ggatgctgag aaagatttct taaaagataa aacaatgcat ttacaattgt
     5401 tatttggaca agcaaatcgt caagatgaac caaatgataa gaaagcaacg agtgttgtgg
     5461 atgtaacatt gaatcataac cttgatggtc gcttatcgaa agatgcatta agccagcaat
     5521 tgagtgcatt atctaggttt gatgcgcatt ataaaatgta cacagataca aaaggcagag
     5581 aagataaacc attcgacaac aaacgtttaa ttgatatgat ggttgaccaa gttatcaatg
     5641 acatggaaag tttcaaagac gataaagtag ctgtgttaca tcaaattgat tcaatggaag
     5701 aaaactcaga caaactgatt gatgacattt taaataacaa aaagaataca acaaaaaata
     5761 aagaagatat ttctaagctg attgatcagt tagaaaacgt taaaaagact tttgctgaag
     5821 agccacaaga accaaaaatt gataaaggca aaaatgatga atttaatacg atgtcttcaa
     5881 atttagataa agaaattagt agaatttctg agaaaagtac gcaattgcta tcagatacac
     5941 aagaatcaaa aacaattgca gattcagtta gtggacaatt aaatcaatta gataataatg
     6001 tgaataaact acatgcgaca ggtcgagcat taggcgtaag agcgaatgat ttgaaccgtc
     6061 aaatggctaa aaacgataaa gataatgagt tattcgctaa agagtttaaa aaagtattac
     6121 aaaattctaa agatggcgac agacaaaacc aagcattaaa agcatttatg agtaatccgg
     6181 ttcaaaagaa aaacttagaa aatgttttag ctaataatgg taatacagac gtgatttcac
     6241 cgacattatt cgtattattg atgtatttac tatcaatgat tacagcatat attttctata
     6301 gctatgaacg tgctaaagga caaatgaatt tcatcaaaga tgattatagt agtaaaaaca
     6361 atctttggaa taatgcgatt acgtctggtg ttattggtgc aactggttta gtagaaggat
     6421 taattgtcgg tttaattgca atgaataagt tccatgtatt agctggctat agagcgaaat
     6481 tcatcttaat ggtgatttta actatgatgg tcttcgtact tattaatacg tatttactaa
     6541 gacaggtaaa atctatcggt atgttcttaa tgattgctgc attgggtcta tactttgtag
     6601 ctatgaataa tttgaaagcg gctggacaag gtgtgactaa taaaatttca ccattatctt
     6661 atatcgataa catgttcttc aattatttaa atgcagagca tcctataggc ttggcgctag
     6721 taatattaac agtacttgtg attattggct ttgtactgaa catgtttata aaacacttta
     6781 agaaagagag attaatctaa tgttgatgaa tagcgtgatt gctttaactt ttttaacagc
     6841 atctagcaat aatggcggac ttaatattga cgtgcaacaa gaagaggaaa agcgaatcaa
     6901 taatgattta aatcaatatg atacaacgct atttaataaa gacagcaaag cggttaatga
     6961 tgcgattgtt aagcagaaaa aagaacgaca acaacaaata aaaaatgata tgtttcaaaa
     7021 tcaagcgagt cactcgactc gcttgaatga aactaaaaaa gtgttatttt ccaaatctaa
     7081 cttagaaaag acttcggaga gtgataaaag cccctatatt caaaacaagc aggagaaaaa
     7141 aatattcccg tacattttga tgtctgtagg ggcttttttg actttaggat ttgtcatttt
     7201 ttcaattcat aaagggagac gaacgaaaaa tgaatcagca cgtaaaagta acatttgatt
     7261 ttactaatta taattacggc acatatgact tagcagtacc agcatattta ccgataaaaa
     7321 atttaatagc tttagtattg gatagtttgg acatttcaat atttgatgtc aatacacaaa
     7381 ttaaagtgat gacgaaaggt caattacttg ttgaaaacga ccgacttatt gattatcaaa
     7441 tcgctgatgg agatattttg aagttactat aggaggaaaa atagatggtt aaaaatcatg
     7501 accctaaaaa tgaaatgcaa gatatgttaa cacctttaga tgctgaagaa gcagctaaaa
     7561 caaaattacg cttagatatg agagagattc ctaagtcttc aattaaacca gaacattttc
     7621 atttaatgta cttattagaa caacattctc catattttat agatgctgaa ttaactgaac
     7681 tacgtgatag tttccaaata cattatgaca ttaatgacaa tcatacacct tttgataata
     7741 ttaaatcatt tactaaaaat gaaaaattac gttacttact caatatcaaa aatttagaag
     7801 aagtaaatcg tacacgttac acatttgtgt tggcaccaga tgaattattt ttcacaagag
     7861 atggattacc aattgctaaa acaagaggtt tacaaaatgt tgttgatcca ttaccggtgt
     7921 cagaagctga atttttaaca agatataaag cactggttat ctgtgcattc aatgagaaac
     7981 aatcatttga tgctttagtt gaaggaaact tagaactaca taaaggaacg ccatttgaaa
     8041 ctaaagttat tgaagcggca acgttagatt tactaacggc atttttagat gaacagtatc
     8101 agaaacaaga acaagattat agtcaaaatt atgcatatgt acgcaaagta ggacataccg
     8161 ttttcaaatg ggttgctatc ggtatgacaa cgttaagtgt tttattaatt gcatttttag
     8221 cctttttata tttttcagta atgaagcata atgagcgtat tgaaaaagga taccaagcat
     8281 ttgtaaagga agattatacg caagtactaa atacgtatga tgatttagat ggtaaaaaat
     8341 tagataaaga agcactttac atttatgcca aaagttatat ccaaacaaat aaacaaggtt
     8401 tagaaaaaga taagaaagaa aatttactta ataacgtgac accaaattca aacaaagact
     8461 acttattata ttggatggaa ttaggacaag gacatcttga tgaagcgatt aatattgcca
     8521 cttatttaga tgataacgat attacaaagt tagcgttgat taataagtta aatgagatta
     8581 aaaataacgg agatttatcg aatgataaac gttctgaaga aacgaaaaag tataacgata
     8641 aattgcaaga tattttagac aaagaaaaac aagttaaaga tgaaaaagcg aaatctgaag
     8701 aagagaaagc aaaagcgaaa gatgagaaat taaagcaaca agaagagaac gaaaagaaac
     8761 aaaaagaaca agcacaaaaa gataaagaaa aacgccaaga agctgaaaga aaaaaatagt
     8821 ataggactga ggcaaagaca atgcataaat tgattataaa atataacaaa caattgaaga
     8881 tgctcaattt gcgagatggt aagacatata ctattagcga agacgagcgt gcagatatta
     8941 cgttgaaatc gttaggcgaa gtcattcatt tagaacaaaa taatcaaggt acttggcaag
     9001 cgaatcatac ttctattaat aaggtgcttg ttagaaaagg tgaccttgat gacattacat
     9061 tacagcttta tacagaagct gattatgcat catttgcgta cccttcgatt caagatacga
     9121 tgacgattgg atcaaatgca tatgatgaca tggtcattca aagtttgatg aatgccatca
     9181 ttattaaaga ttttcaatcg atacaagaaa ctcaatatgt gcgcatcgtg cacgataaaa
     9241 atacagatgt gtatattaac tatgaactac aagagcaatt aacgaacaaa gcttacattg
     9301 gtgatcatat ttatgttgaa gggatatggc tcgaagtaca agctgatggt ttaaatgtat
     9361 tgagtcagaa tacagtggca tcgtcattaa ttcgcttaac acaagagatg ccacatgcac
     9421 aggcagatga ttacaatacg taccatcgtt cgccaaggat tattcaccgt gaaccgaccg
     9481 atgatattaa gattgaaaga ccaccacaac caatacagaa gaacaataca gtgatatggc
     9541 gttccattat accgccatta gtaatgattg ctttaactgt tgtcatcttt ttagtgagac
     9601 caattggtat ttatatttta atgatgattg gtatgagtac agtaacgata gtatttggta
     9661 ttacaacgta tttctctgaa aagaaaaagt ataacaaaga tgttgaaaaa cgagagaaag
     9721 attacaaagc ttatttggat aataaatcta aagagattaa taaagcgatt aaagcacaac
     9781 gttttagttt gaattatcat tatccaacgg ttgctgaaat taaagatatc gttgaaacga
     9841 aagcaccaag aatatatgaa aaaacatcgc atcatcacga tttcttacat tataagttag
     9901 gtattgcgaa tgtagaaaag tcattcaaat tagattacca agaagaagaa tttaaccaac
     9961 gtcgtgatga actattcgac gatgctaaag aattgtatga attttacaca gatgtagaac
    10021 aagcaccatt aatcaatgat ttaaatcatg gaccaattgc atatattggt gcacgacatc
    10081 tcattttaga agaattggag aaaatgctaa tccaattgtc aacgttccat agttatcatg
    10141 atttagagtt tctatttgtg acacgtgaag atgaagttga aacattgaaa tgggcacgtt
    10201 ggttgccaca tatgacattg agaggtcaaa acattagagg gtttgtttac aatcaacgaa
    10261 cacgtgacca aattttaacg tcaatttata gcatgattaa agaacgtatc caagctgtgc
    10321 gtgaacgcag cagaagtaat gagcaaatta ttttcacacc gcaattagtg tttgtcatta
    10381 cagatatgtc attaattatt gatcatgtca ttttagaata tgtaaaccaa gatttatcag
    10441 aatatggtat ttcattaatc tttgttgaag atgtgattga aagtttgcca gagcatgtag
    10501 ataccattat tgatatcaag tctcgtactg aaggcgaact gattacgaaa gaaaaagaat
    10561 tagttcaatt gaaatttaca cctgaaaata ttgataacgt tgataaagaa tatatcgcga
    10621 gacgtttggc gaatttgata cacgtcgaac atttgaaaaa tgcaattcct gatagtatta
    10681 catttttaga gatgtataac gtgaaagaag tagatcagct tgatgtggtt aatcgatgga
    10741 gacaaaacga aacatacaaa acgatggcag tacctttagg tgtaagaggt aaagatgaca
    10801 ttttatcatt gaacttacat gaaaaagcac acgggccaca tggtttagtt gctggtacca
    10861 ctggttcagg gaaatctgag attattcaat catacatttt atctttagct attaattttc
    10921 accctcatga agttgcattc ctattgattg actataaagg tgggggtatg gcgaacttat
    10981 ttaaagattt agtccattta gttggtacga ttacaaactt agatggcgat gaagcgatgc
    11041 gtgccttaac atcaatcaaa gccgaattga gaaaacgtca acgtttattc ggagagcatg
    11101 atgttaacca tattaatcaa taccataagt tatttaaaga aggtgttgcg acagaaccaa
    11161 tgccacattt attcattatt tccgatgagt ttgcagaatt aaaatcagaa caacctgatt
    11221 ttatgaaaga acttgtatca acggcacgta ttggacgttc gttaggtatt catttaatac
    11281 ttgcgacaca aaaaccatcg ggtgttgttg atgaccaaat ttggtctaac tctaaattta
    11341 aattggcatt aaaagtacaa gatagacaag acagtaatga aattttaaaa acaccagatg
    11401 cagcagacat tacattacca ggtcgtgcgt atttacaagt tggtaataat gaaatttatg
    11461 aattattcca atctgcatgg agtggtgcaa catatgacat tgaaggcgat aaattagaag
    11521 ttgaagataa gacgatttac atgattaatg actatggtca acttcaagca atcaacaaag
    11581 acttgagtgg acttgaagat gaagaaacaa aagaaaatca aactgagtta gaagcggtta
    11641 tcgatcatat cgaatctatt acaacacgat tagaaattga agaagttaag cgtccatggt
    11701 taccaccatt accagaaaat gtatatcaag aagatttagt agaaacagat ttcagaaaat
    11761 tatggtcaga tgatgcaaaa gaagtggaat taacattagg acttaaagac gtaccagaag
    11821 aacaatatca aggaccgatg gtattgcaat tgaaaaaagc tgggcacatc gcgttaatcg
    11881 gaagtccagg atatggtaga acaacgttct tacacaacat tattttcgat gttgcaagac
    11941 accatcgtcc tgatcaagca catatgtact tgttcgattt cggtaccaat ggtttgatgc
    12001 cagtcacaga tataccacat gtcgctgatt actttacagt agatcaagaa gacaagattg
    12061 cgaaggcgat acggaagata catgatatta tttctgagcg aaaaagacta ttaagtcaag
    12121 agagagtagt taatatagag caatataata aagaaactgg aaattcaatt ccaaatgttt
    12181 tcttaattat cgataactac gacacggtga aagaatcacc atttatggaa gaatatgaag
    12241 aaatgatgtc taaggtaaca cgtgaaggtt tagcgttagg ggtatacatc attctttcag
    12301 gatcaagatc aagtgcaata aaatcagcta tatttactaa cattaaaaca agagttgcgc
    12361 tgtacctctt tgaaaacaat gaattaacaa atattattgg ttcttataaa aaaggtgtga
    12421 aagatgttaa aggtagagct gcaataaatg atgataactt cacacagttc caaattgcgc
    12481 aaccatttga attagcagaa ggacaaacat ataacgagcg cattaaaaat gaagtagcac
    12541 aaatgaaaga attctatgta ggagattatc caaaacatat tccaatgatg ccagataagg
    12601 tatttatgga agatattcga gaagcatatg atttggaaaa aatcattcat gaagaacaca
    12661 aattaccatt aggattagac tttgaggatg tggaactagt tagtttagat ctgacaagtt
    12721 cttcgattgt aacagcaatt aaaccaactg agatggaaaa aatgaatgat gttataatgt
    12781 ctagtttatc agtatatagt aaaaaccaat ttgttatctt ggttgatgca gaagataata
    12841 tgagccagta ttcagaagat gtaacctctt attattcagc accatcagat ttgagcaaca
    12901 ttagattagg atttaagcaa gaaatagaag caaggaaaaa tggcgagaaa agtatagagg
    12961 aatgcaagat tgtatttata aataatatta aacgattcaa tcaattaact ggaatgacag
    13021 aagatgaaat tcgtgtgtta tttaatgaag gtcaaaaagt taatattatc attattgcaa
    13081 gtggactata ctcagataca attggtgcat ttgatagaga aagtaaaatg atggtaagaa
    13141 ctataaatca agctttgatt agtcataaga tatcggaaca agaatttata agagttaaag
    13201 atcgatttgg tgaaccagaa ttaaaagtgg gagaaatgta ttacattaat aatcaagaat
    13261 atcaaaaaat aaaactaatg gagggataga gttgataaag ttaaaccaag cctctgtcag
    13321 caaagaaatt tcaagcattc gaacaaatgg acaaggctta aaacaatcaa atgggaatgt
    13381 aaatttaagt aaaacgaatc tcgttacttt taaagaatat gtaaatatgt ttgaagatta
    13441 tcaatcagct ctatctaatt atgaaaacat aattgaacaa gatacgacgg ctatggatac
    13501 aacggtaaca gaaattgttg aaaacgatag agaaatagca ggacaaatta ataaataatt
    13561 aaacaagacg ggtgagaaaa atgagtaata agggagaaat aagaaggcaa attgctaata
    13621 aagaaagaga aaaagcaagt aaagaagcac aactaacaga tttaaaagag gatttaagaa
    13681 ggttgaaaga cgcttcgaaa aaattggata cagcaggaga agattttaac aaaggacaga
    13741 gtagttataa caaagtagaa attagtactt cagattggaa aggagaaaga aggactaaaa
    13801 gtgacagcaa aaagaaagat gtcgattctg aattaaaaaa agttgaacaa gactttgatg
    13861 atgcgaagaa ggctattaaa aaagatattc aggataaaga agaagaaatc aagggagtcg
    13921 aaggagaaat cagtactata aatgcagcta tagatgcact taaatcaaaa ttataataat
    13981 agagagggaa atttattatg gggtacaaag ttgatatgtc tgaagtgcac aatatgcaaa
    14041 agtctattga ttcaagctta agtgcaataa atacaaaggt tagcacgctt agcagtagca
    14101 taaacaacct tataaatact gaaggattcg aaggtcagac agcaagtagt gtcaaaaact
    14161 atagtaagac atttcattta caatcaataa aaaagattga aacaataaac aaagatttta
    14221 aatcagatat tgcgaaatct atacgtaaat tccaaagtga agttgataat aatgcttcag
    14281 ctatattgga tgaagacgaa ataaaaaaat acaaaaaaga tattgatgat gcattaaaag
    14341 atgttttcaa atcttctaaa gatgcaaacg gagctatttc agatgtttca gatttaacaa
    14401 cagccaaaaa aataaagaca gaaaacttgg ccaataaaat gggagatttt aataaagata
    14461 ttgatcaaac ggtggaaaga ttaacgacct tcgacgccaa caattctata gatggtgata
    14521 agacagataa cttaataact gaattaagtg gtgtaaattc atatgtcaaa aacatgaagc
    14581 caaaccgtgc aagaattagt tctacaagtg gaaaaattga aggtgcaatt gcaaggcata
    14641 aaacaagtga agagttagta agatggcaaa cttacatgga aacgttaagt gaaaatcttt
    14701 ataaaacacc aggtctaagt aaagcagctt ctgacataat tacccaagct ggacgcgagt
    14761 actatgctat caaagctgta gggaatggca gtgctttaag aggatataaa gaatatctga
    14821 aaacacgaga tattaataaa ttaattaaca aaatgaataa aaaccaatta aaaagaatgt
    14881 cattggtatt aaatacagat agaggtaata ttaaaataaa aaatgcagta aaaatggcaa
    14941 gtgaatttgc ccgtaataat ccttttaaaa aaggaaattt agtaaattgg atgagcaaag
    15001 tccaagggta tcaaagtgta agtgctcaaa tattaaaaaa ggggttgcaa gataagaact
    15061 ttaaatatac atttggagat gcaaagaaat tttttgatac agctgatatg aaaaaagcag
    15121 cagtcacaga atttaaaaat acctttgtac cacaaaatat aagagaaacc ttctttaaga
    15181 aagaaaattt aacatctaag acagcattaa agaaaaatat gagtaaattc tggaatgaag
    15241 atattaaggg tggaatcaaa gagtttaaat tagactttgc taacaaaaat atcattggta
    15301 aactcggcaa attgttgaaa ctaggaggca aggctttaaa accattagga tttattactg
    15361 caattactga caacatagga aagaaatctt ggcaagaaaa attagttggt atgggggtag
    15421 atttaagtgc tattggtgca agtgcagctg caggtgcagc tgttggaacg gcaataccat
    15481 tacctgtagt aggaacagtt gcaggtgcat tagctggagc agcagtgggt atagctttag
    15541 attataaaat accgtatctt gataagtctg caacagaatt agctaaagat ggtattaact
    15601 cgggtattaa caaagttaaa agtggatttg gaaaagtaac ttcaggattt aaaacagttt
    15661 tcggataaga aaggggcaga caaatgaaaa tacaagagat tgtaggaaat ataaagagag
    15721 cttatctgac accattaata atttttgtga ttttgttttc attatttttt gatttgataa
    15781 tgtattttta tggaaacctt caagggaaat tgccaatata tttagatgta tttctaatat
    15841 ttgcagcagt tgtatttatt ttaatgtact tccaagagaa aagtggagaa gttaaggttg
    15901 aaaaatcaaa cgttattcgt tatttaacat taaatgttgt agctgggtat tcaatggtac
    15961 tacttgttgc ctctatttat gtttttgggg tagcaggcta tggttttgat gtatttaatt
    16021 actggttagg tattatatta atgctatttg tatcgtggtt tgctctattt ttgttttata
    16081 aaaatgaatt tgatagtgaa aatccaaata aaacagttaa tgttatagca attattataa
    16141 agttatctgc ttttggagga cttttctata tcagaacagt cgttcctaat actgcagatg
    16201 aagagaaatt tataactcta agtatcttaa ttaatatagc tgttgattta ttgctggtta
    16261 gatcatattt taactatgca ttatataaga gtgtaaaaaa agatatcgaa aacggagtgg
    16321 atgattaatg gaagtaggaa tgttaattat gccgttttgt gtgaagatta catttgaaat
    16381 tgataaaaat gataattggt taaatgcatt agatacagta aatgaatttt tcatggacaa
    16441 agatatatat ccaactggtc ctataatttt ccaaagagaa ttagcaggtt tgggtgaata
    16501 taaatataca gcatatatag ctcttaatga tgaacttcaa gatatacctg aattgaacat
    16561 caaatatatt gattgtttag aggtaggacc tacgttatca actaagtgtt ttgaagaaga
    16621 ggaatttgaa agagcttata aagaaattga atatgtagct aacgaaaata atattaagat
    16681 cctaaatcaa ccatattatc atgtaatggt agattatttt ggtggtacgg cttttgaaat
    16741 ttatgctcaa gtagatttgg atgagagtga agtatatgga taaaaatgga tactcatata
    16801 ttatgatgaa aaatgtagct tacaaaactt attttgatgt gactatggat gactatgaag
    16861 atatattaaa agactttaca aatcttgcaa tttctaaagg taaaacaatt acagggccat
    16921 taacctttgc agtcacgcaa atagacttgc aaaaaaggat gaatattgat ttatttattt
    16981 cagttggaaa atgttttaaa tcaaatgaag aactatcata tagaacatat ttttgtctag
    17041 ataacatgtt acatggtcgt atcacttcca acaattttat aacagatgaa atagcgttac
    17101 ttgaggatat gaacgaattt gcttctgaca acaatttaac gtttacttcg ccttattatc
    17161 acacaatgag aaaaagtttt agcggtgaac aaggttggat tgatgtaaag gccaaagtgt
    17221 atgaaaatga ttaattaata atcagtagag gtaatatatg ataattttta ttttattaac
    17281 agtttttgca ctcttttata tagcgatgat tgctagttta tttaaaagtg aaggtttttc
    17341 aataataggc ttaatattgg atatagttat tttgacaacg ttaatatttt attattttgt
    17401 aggtgcgagt tttgttgata atgatttaag taacttttta gcattcatga attttgggtc
    17461 atttgtgtat atgtactatg caattaagag cttgtgggtg aagcctaagt tagtaaatta
    17521 tataattgca aaagaaatag gcgaatcaaa agatgtcatt gaagaacagg aattagattt
    17581 acagacatca aagataagag gtatctactt tttcattatt gcaattgcct tgttaataat
    17641 tactaaatta agaatgcaac ctgagttaca ggcagatgct atatcgatga atccagtttt
    17701 tatttttata ggagttatta tcattttaat ttggctagta ctggatatat atcgtaagaa
    17761 aaaatacggt atattcttat tcaagacgat agtgccatta gttgttacga cttggattat
    17821 tatagctact attgtacttt cataagaaat tttcaataat ttttttggtt ataattgttg
    17881 atggatatta attaatcgat agaaaaatct taatgatttt tgaaaaatat gtaaaaagta
    17941 tatactatta atatcttttt tactataatt ataaatagtt aaataaattg aaagacaaaa
    18001 taatgagtag gagttaatga gatggaaaac caaaaacaag gcaatggctt aaaaattgca
    18061 acatgggtat ttattgtatt aacagtagtt acaccgctat ttggtattgg aagtattgtt
    18121 tgtagtatta attacaaaaa atacgatgca gaaaaaggtt cgaagttatt gaaaattgca
    18181 attatcgtaa caataattgt ttttgtttta aatttattag catatttagg tttaagataa
    18241 gtaaaatgaa tttgaagaag cattaagcga catttgggtg ttgtttaatg ctttttattt
    18301 aacggaaata agtgtagaga ataaattaat agcttaatca agagatattt cgaacacata
    18361 ggggcgataa catgactttc gaagagaagt taagtcaaat gtacaacgag attgcgaatg
    18421 agatcagtgg gatgataccg atagagtggg aaaaagtata tacaatggct tatatagatg
    18481 atgaaggtgg agaagtgttc tactattaca cagaacctgg aagcaatgaa ttatactact
    18541 atactagtgt attaaataaa tatgatatat cggaatcaga atttatggac tcagcgtatg
    18601 agttgtataa acaatttcaa aatttaagaa atatatttaa agaagaagga tatgaaccat
    18661 ggacatcatg cgaatttgat tttacaaaag aaggtgaatt aaaagtttca tttgattata
    18721 tagattggat caatacagag tttgatcaat tgggccgtca aaattattat atgtacaaaa
    18781 aatttggggt tataccagaa atggaatatg aaatggaaga agttaaagaa atcgagcaat
    18841 atattaaaga gcaagaagaa gctgaacaat aggggagata acatgacttt cgaagaaaaa
    18901 ttaagtgaaa tgtacaatga aattgcgaat aaaattagta gcatgatacc agtagaatgg
    18961 gaaaaggtat atacaatggc ttatatagat gatggaggag gtgaagtatt ctttaattat
    19021 actaaaataa acagcgatga attgaattat tacaccgata tacctaagga gtataacatt
    19081 tctgtgcaag tatttgatga tttatg
//
LOCUS       DAMZPP010000042        18962 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMZPP010000042 DAMZPP010000000
VERSION     DAMZPP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18962
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.042"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..1469
                     /locus_tag="POH73_001127"
     CDS             <1..1469
                     /locus_tag="POH73_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDI6887093.1"
                     /translation="LFAQYTNYAPSAVRAIIMTTLVLLITNQIKIKGIQLLAFAFIIM
                     FILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFIAQLASFIVAIPNF
                     HQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYLVDLSFNFHDWLLD
                     LFTRIKQSHFSVPKFNDWIFIIFIISVYYIFWLLAKRKYILVTFWTIIILTLLITFPT
                     NSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYSISKYHILPTLNER
                     GINELEYLILTHPHNDHIGELEYIISHIKIKHIVIYNKGYSSNTLMLLSKLSHKYNIK
                     LMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKKVLLMGDASKNNES
                     LLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKNNMYHLPNIEVVKR
                     LQRIRSRIYNSQQNGQVTIDLDDNLKVDSNSYGNASGL"
     gene            1526..2500
                     /gene="holA"
                     /locus_tag="POH73_001128"
     CDS             1526..2500
                     /gene="holA"
                     /locus_tag="POH73_001128"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDI6887094.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKSPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELFILSL"
     gene            complement(2545..2796)
                     /locus_tag="POH73_001129"
     CDS             complement(2545..2796)
                     /locus_tag="POH73_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDI6887095.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            3142..4965
                     /gene="lepA"
                     /locus_tag="POH73_001130"
     CDS             3142..4965
                     /gene="lepA"
                     /locus_tag="POH73_001130"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDI6887096.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCILRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            5472..6596
                     /locus_tag="POH73_001131"
     CDS             5472..6596
                     /locus_tag="POH73_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDI6887097.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRNLKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKFQL
                     LEKYGVNRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSRRFKKKFDQSIESVFG
                     QTINNLKEKKLIVEKNDAIALTNRGKVIGNEVFEAFLIND"
     gene            6697..7674
                     /gene="hrcA"
                     /locus_tag="POH73_001132"
     CDS             6697..7674
                     /gene="hrcA"
                     /locus_tag="POH73_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDI6887098.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFSNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            7706..8332
                     /gene="grpE"
                     /locus_tag="POH73_001133"
     CDS             7706..8332
                     /gene="grpE"
                     /locus_tag="POH73_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDI6887099.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            8401..10233
                     /gene="dnaK"
                     /locus_tag="POH73_001134"
     CDS             8401..10233
                     /gene="dnaK"
                     /locus_tag="POH73_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDI6887100.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            10369..11508
                     /gene="dnaJ"
                     /locus_tag="POH73_001135"
     CDS             10369..11508
                     /gene="dnaJ"
                     /locus_tag="POH73_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDI6887101.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINDQPSNFKDRAKRFFKGE"
     gene            11512..12450
                     /gene="prmA"
                     /locus_tag="POH73_001136"
     CDS             11512..12450
                     /gene="prmA"
                     /locus_tag="POH73_001136"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDI6887102.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSYDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNELTYDDKLRQRIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASYLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIDDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            12452..13204
                     /locus_tag="POH73_001137"
     CDS             12452..13204
                     /locus_tag="POH73_001137"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDI6887103.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIAVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAHEEEAKDGELSNFKQTLQQFKAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            13211..14557
                     /gene="mtaB"
                     /locus_tag="POH73_001138"
     CDS             13211..14557
                     /gene="mtaB"
                     /locus_tag="POH73_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDI6887104.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEEEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            14850..15026
                     /locus_tag="POH73_001139"
     CDS             14850..15026
                     /locus_tag="POH73_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDI6887105.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
     gene            15270..15953
                     /locus_tag="POH73_001140"
     CDS             15270..15953
                     /locus_tag="POH73_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDI6887106.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
     gene            15971..16960
                     /gene="floA"
                     /locus_tag="POH73_001141"
     CDS             15971..16960
                     /gene="floA"
                     /locus_tag="POH73_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HDI6887107.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            16977..17675
                     /locus_tag="POH73_001142"
     CDS             16977..17675
                     /locus_tag="POH73_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HDI6887108.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            17978..18925
                     /locus_tag="POH73_001143"
     CDS             17978..18925
                     /locus_tag="POH73_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDI6887109.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGTVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
BASE COUNT         7076 a         2560 c         3558 g         5768 t
ORIGIN      
        1 ccttatttgc tcaatacaca aattatgcac ctagtgctgt aagagctata ataatgacaa
       61 cgcttgtact gcttattact aatcaaatta aaataaaggg tattcagctt ttagcatttg
      121 catttataat tatgtttatt ttaaatccac tagttgttta tgatattgga tttcaatttt
      181 cattcatcat ttcatttttt attatgttac tttttccttt tttacagcaa ttgtcgaagt
      241 tacaatcatt attcataatt acgtttattg cacaattagc ttcatttatc gttgccattc
      301 caaactttca tcaacttcaa tgggtgggat ttttatctaa tttgattttt gtaccatact
      361 attcgattat attgtttcct ctatctattt tattctttat tacaagtcat tttattgtgg
      421 gattaacgcc gctaaattac ttggttgacc taagttttaa ttttcatgac tggttactag
      481 acctattcac aagaatcaag caatcacatt tttctgttcc caagtttaat gattggatat
      541 ttataatatt tataatttcc gtttattaca tattttggtt attggctaaa cgtaaatata
      601 tattggttac gttttggact ataattattc tgacattatt aataacgttt ccaacaaatt
      661 cacatcacaa aattacaatg ttaaatgtgg ggcagggaga cagtatttta tatgaaggtg
      721 gtaagaacca aaatgtcttg attgatacag gtgggaaagt gattgatgat actaaacaac
      781 ctagttattc aatttctaaa tatcatattt taccaacgct aaatgagaga gggataaatg
      841 aattagagta tctaatttta acacatccac acaatgacca tattggtgaa ttggaatata
      901 ttattagtca tattaaaatt aaacatatag tgatatacaa taagggatat agtagtaata
      961 cattgatgtt attatcgaaa ttaagccata agtataacat taaacttatg gatgtaaggc
     1021 aagttagtag ttttaaactt ggagatagta gttttctatt ttttgatagt tttattccaa
     1081 atagccgaga taaaaatgaa tactcgatta ttactatgat tacatatcaa aataaaaaag
     1141 ttttattaat gggcgatgct agtaaaaata atgaatcttt acttctaaaa aaatataatt
     1201 tgccggagat tgatatttta aaagtaggac atcatgggag taagacaagt agttctaaag
     1261 aatttataga gatgattaag cctaaaataa gtttgatttc ttctggaaag aacaatatgt
     1321 atcatcttcc taacatagaa gttgttaaac gattgcaaag gattcgcagt cgcatttaca
     1381 atagccaaca aaacggtcaa gttacaatag acttagatga taatttaaaa gttgattcaa
     1441 actcttatgg aaatgcaagt ggtttataga tacagaaaca tgatataatc tattgatgtg
     1501 tcaaacgaat gaaagggtgt actgaatgag cgacaatatt gtagctattt atggagatgt
     1561 gcctgaattg gttgaaaaac aaagtgcaga aatcatatca caatttttga aaagtgatag
     1621 agatgacttt aactttgtga aatataattt atacgaaaca gagattgcac caattgttga
     1681 agaaacatta acattgcctt tcttttcaga taaaaaagca attttggtta aaaatgcata
     1741 tatatttaca ggtgaaaaat cgccaaaaga tatggctcat aatgtagatc aattaataga
     1801 atttattgaa aaatatgatg gcgaaaattt gattgtcttt gagatatatc aaaataaact
     1861 tgatgaaaga aaaaagttaa ctaaaactct aaaaaagcat gcaaggctta aaaaaataga
     1921 gcaaatgtct gaagaagaaa taaaaaaatg gattcaaagt aaattaaatg agaatttcaa
     1981 agatatcaaa agagatgcat tagatttatt tattgagttg acaggtatta actttaatat
     2041 tgtctcacaa gagatagaaa agttgatttt atttttaggc gatagaccaa caattaataa
     2101 gcaggatgtt aaccaaatta ttaatagaag tttagaacaa aatgtatttt tactgactga
     2161 atacattcag aaaagaaaga aagaacaagc aattcattta gtaaaagatt taataacaat
     2221 gaaagaagaa cctattaaat tacttgcact aattacaagt aattaccgat tattttatca
     2281 atgtaagatt ctgagtcaaa aaggatatag tggacagcaa attgctaaaa caataggcgt
     2341 acatccatac agagtaaaat tagcgttagg acaagtaaga cattatcaac ttgatgaatt
     2401 attgaatatt atagatgctt gtgcggaaac tgattataaa cttaaatcat catatatgga
     2461 taaacagtta atactggaat tattcattct atctttataa aaaataaaag ttcaagcatg
     2521 cgcttgaact tttatttaaa aagattattt atttgcagtc attaattgtg atttaatacg
     2581 gtcagctttg tttgaatgta ttaaattact ttgagcagct ttgtctacta acttaactgc
     2641 taagcttact aattcatttt tattatcagc gttatttgaa acagctgttt tagcgttttt
     2701 aactgctgta cgcattgcac tcttttgtga aatgttgcgt gcttcagctt tttcagttgt
     2761 ttttacacgt ttaattgcag atttgatatt tgccatttct gtcacctcct aaaagtgagt
     2821 tcatcttatc aaaattttat ttgattacaa caagaaatat tttaacaaaa gtctaaccgt
     2881 tgtgcaatca tttatttgat ggggttagct taaaattttg cagatttaat aaattgtgtt
     2941 ggaaacacaa tcaatacgtt ttataattat aagtacgtaa ttaatatatt aacatatcgc
     3001 aagtatgtat ttaaataaag attgttataa attcaaagtt catccaagat tatggcgttt
     3061 gcatttacct attaaaaacg ttattatatc aaagatgcga aagataatac gggtttattt
     3121 tatgaaagtg agaaggataa aatggataat gagcaacgct taaaaagaag agagaatata
     3181 aggaatttct cgattatagc acatattgac cacggaaaat ctacattggc tgatagaatt
     3241 ttagaaaata ccaaatcagt tgaaacaaga gatatgcaag atcagttact agattcaatg
     3301 gatttagaaa gagaacgtgg tattacaatc aaattaaacg cagtacgttt aaagtacgaa
     3361 gctaaagatg gaaatactta tacattccat ttaatcgata cgcctggaca cgtcgatttt
     3421 acatatgaag tgtcacgttc tttggcagct tgtgagggcg cgattttagt agtagatgcg
     3481 gctcaaggta tcgaagcaca aacattagca aatgtttatt tggcattaga taatgagtta
     3541 gagttattgc ctgttattaa caaaattgat ttacctgctg cagaacctga acgcgtgaaa
     3601 caagaaattg aagatatgat aggtttagac caagacgatg ttgttttagc aagtgctaaa
     3661 tctaacattg gaattgaaga gatacttgag aaaatagttg aagttgtgcc agctccagat
     3721 ggtgacccag aagcaccact aaaagcgtta atatttgatt ctgagtatga tccatataga
     3781 ggggtaattt catcgataag aattgtagac ggtgttgtta aagccggaga taaaattcga
     3841 atgatggcca ctggtaaaga gttcgaagta acagaagttg gaattaatac acctaagcaa
     3901 cttccagttg atgaattaac agttggtgat gttggttaca ttattgcaag tattaaaaat
     3961 gttgatgatt ctagggttgg tgacacaatc acattagcta gtagacctgc atcagaacca
     4021 ttgcaaggtt ataagaaaat gaatccaatg gtatattgcg gactgttccc aatagataac
     4081 aaaaattata atgatttaag agaagcatta gaaaaattac aattgaatga tgcatcatta
     4141 gaatttgagc ctgaatcatc acaagcatta ggttttggtt atagaactgg tttcttaggt
     4201 atgttacaca tggaaataat tcaagaaaga attgaaagag aatttggcat tgaattaatt
     4261 gcaaccgcac catctgtaat ttatcaatgt attttaaggg acggttcaga agtgacggtt
     4321 gataatccag cacaaatgcc agaccgtgat aaaattgata aaatattcga gccatatgtt
     4381 cgtgcaacta tgatggttcc aaatgactat gtaggtgcag taatggaatt atgtcaacgt
     4441 aaacgtggac aatttataaa tatggactat ttagatgata ttcgtgtaaa tattgtttat
     4501 gaattacctt tagctgaagt tgtatttgat ttcttcgatc aacttaaatc taatactaaa
     4561 ggatatgcat catttgatta tgagttcatc gaaaataaag aaagtaattt agtgaagatg
     4621 gatattttat taaacggtga taaagtggat gctctaagct tcatagtcca tagagatttt
     4681 gcatatgaac gtggtaaagc attagttgaa aaacttaaaa cgttaattcc aagacagcaa
     4741 tttgaagtac ctgtacaagc tgcaatagga caaaaaattg tagcacgtac aaatattaaa
     4801 tcaatgggta aaaacgtttt agctaaatgt tatggcggtg acataagccg taaacgtaaa
     4861 ttacttgaaa aacaaaaagc aggtaaagct aagatgaaag cagttggtaa tgttgaaatt
     4921 ccacaggatg cttttttggc tgtattgaaa atggatgacg aataattttt taaaaatcaa
     4981 ttaacaattt acaatgaata aagtttaata actaaaaaga gggagcctag gataaattaa
     5041 cgtcctgggc tttacaatgt tatattggca gtcatcgaca gagttaaaat gagcttataa
     5101 caatggggcc ccaacataga agctgacgaa aagtcagctt acaataatgt gcaagttggg
     5161 gtgggacgac gaaataaatt ttgcgaaaat atcatttctg tcccactccc ttatgcatga
     5221 gtttaactca tgtaattcta tttttaagta catattacct ctggctaatg tgtaagagcc
     5281 actacataat aaatcattag tggttcttta ttatttctat ctcactccct cttaacattt
     5341 ataaatatta aaatgaatcg atatattaga caattattga ttaaacgtta aagttaaaag
     5401 taagaataat tgcagatagt ccaacaggat atagccgatt ggataaacag tctgagaagc
     5461 ggggcattaa aatgacagta caaagtgcat atatacatat tccattttgt gtaagaatat
     5521 gtacatattg tgatttcaat aaatatttta tacagaacca acctgtagat gagtacttag
     5581 atgcactaat aacagaaatg tctacagcaa aatataggaa cttaaagact atgtatgtag
     5641 gtggcggcac accaacggcc ctttctatta atcagttgga aagattactt aaagcaatac
     5701 gtgatacgtt tacaatcaca ggtgagtata catttgaagc aaatcctgat gagttaacta
     5761 aagagaaatt ccaactatta gagaaatatg gagtaaacag aatttcaatg ggcgttcaaa
     5821 cattcaagcc ggagttattg tctgttttag gtagaacgca caatactgaa gatatttaca
     5881 cgtcggtgtt aaatgctaaa aacgccggta ttaaatcaat cagtttagat ttaatgtatc
     5941 atttaccgaa acagacgatt gaagattttg aacaaagttt agatctagct ttagatatgg
     6001 atattcaaca tatttcgagt tacggcttaa tacttgaacc taaaacccaa ttttataata
     6061 tgtatagaaa aggtttgctc aaacttccta atgaggattt aggtgctgac atgtatcagt
     6121 tgctgatgtc taagatagaa caatctcctt tccatcaata cgaaatatct aattttgcat
     6181 tagatggcca tgaatcagaa cataataagg tttactggtt taatgaggaa tattatggat
     6241 ttggagcagg tgcaagtggt tatgtagatg gtgttcgtta tacgaatatc aatccagtga
     6301 atcattatat caaagctata aataaagaaa gtaaagcaat tttagtatca aataaacctt
     6361 ctttgactga gagaatggaa gaagaaatgt ttcttgggtt gcgtttaaat gaaggtgtga
     6421 gtagtagaag gttcaaaaag aagtttgacc aatcaattga aagtgtcttt ggtcaaacaa
     6481 taaataattt aaaagagaag aaattaattg tagaaaagaa cgatgcgatt gcacttacaa
     6541 atcgagggaa agtcattggt aatgaggttt ttgaagcttt cctaataaac gattaaaaaa
     6601 aattgaaatt tcgagtcttt aacattgact tactttgacc aatttgataa attataatta
     6661 gcacttgaga taagtgagtg ctaatgaggt gaaaacatga ttacagatag gcaattgagt
     6721 atattaaacg caattgttga ggattatgtt gattttggac aacccgttgg ttctaaaaca
     6781 ctaattgagc gacataactt gaatgttagt cctgctacaa ttagaaatga gatgaaacag
     6841 cttgaagatt taaactatat cgaaaagaca catagttctt cagggcgttc gccatcacaa
     6901 ttaggtttta ggtattatgt caatcgttta cttgaacaaa catctcatca aaaaacaaat
     6961 aaattaagac gattaaatca attgttagtt gagaatcaat atgatgtatc atcagcattg
     7021 acatattttg cagatgaatt atcaaatata tctcaatata caactttagt tgttcatcct
     7081 aatcataaac aagatattat caataatgta cacttgattc gtgctaatcc taatttagtt
     7141 attatggtta tcgtattttc atcaggtcat gttgagcatg tacatcttgc ttcagacata
     7201 ccgtttagta atgacaaact aaatacaata tcaaattttg ttactaacaa actaactgag
     7261 tttaatcaga atttacaaga tgatattgtc tcttttgttc aatcagagca ggaagaaata
     7321 tttatcaata aattgttaaa tacgatgaat aatcatattt ctaatcaaag taatagcatt
     7381 tatatggggg ggaaggttaa acttattgat gcattgaatg aaagtaatgt atcttcaatt
     7441 cagcccattc tacaatatat agaatcaaat agaattgcag aattattaca agatatatct
     7501 tcaccaaata ttaatgtaaa aattggtaat gaaattgatg acagcttaag tgatatttca
     7561 attgtaacaa gtcaatatca ttttgatgaa acgttaaaag gtcaaatcgc tgtgattgga
     7621 cctacagcta tgcattatca aaatgtcatt caattattaa atagaatttg gtaatgatgg
     7681 attagaaagt attggaggac agaaaatgac aaataaagac gaatcagttg aaaaaaacac
     7741 tgaatcaaca gttgaagaaa caaacgtcaa acaaaatatt gatgattcag ttgaacaagc
     7801 tgaagaaagt aaaggtcatt tacaagatga agcaatagaa gaaacgtctg acgagaatgt
     7861 tattgaagaa atagatccaa aagatcaaaa aattaatgaa cttcaacaat tagcagatga
     7921 aaacgaagag aaatatttaa ggctctacgc tgagtttgaa aattataagc gtagaattca
     7981 aaaagaaaat gaaataaata aaacatatca agcacaacgt gtgttaacag atattttacc
     8041 agcaatagac aatatagaac gtgcacttca aattgaaggt gatgatgaga cttttaaatc
     8101 tcttcaaaaa ggtgtacaaa tggtgcatga aagtttgatt aacgcactaa aagataatgg
     8161 tcttgaagtt attaaaactg aaggtgaagc atttgatcca aatattcacc aagctgtagt
     8221 tcaagatgat aaccctgatt ttgaatctgg cgaaatcact caagaactac aaaaaggata
     8281 caagcttaaa gatagagtat taagaccatc aatggtcaaa gtaaaccaat aacttaaatt
     8341 tggcgaaaag acattgttta aaattaaatt aatttaatga ttaaatggag gaattttatt
     8401 atgagtaaaa ttattggtat agacttaggt acaacaaatt catgtgtaac agtattagaa
     8461 ggcgatgagc caaaagtaat tcaaaaccct gaaggttcac gtacaacacc atctgttgta
     8521 gctttcaaaa atggagaaac tcaagttggt gaagtagcta aacgtcaagc tattacaaac
     8581 ccaaacactg ttcaatctat taaacgtcat atggggactg attataaagt agatattgaa
     8641 ggtaaatcat acacaccaca agaaatctca gctatgattt tacaaaactt aaaaaataca
     8701 gctgaaagct atttaggtga gaaagttgac aaagctgtaa ttacagtacc tgcatacttt
     8761 aacgatgctg aacgtcaagc aactaaagat gctggtaaaa ttgctggttt agaagttgag
     8821 cgtatcatta atgaaccaac agctgcagca ttagcatatg gtttagacaa aactgataaa
     8881 gacgaaaaag ttcttgtttt tgacttaggt ggcggtacat ttgacgtatc tatcctagaa
     8941 ttaggtgacg gtgtattcga agtactatca acagccggtg acaacaaact tggcggtgat
     9001 gattttgacc aagtaattat tgactaccta gttgcagaat tcaaaaaaga aaatggcgta
     9061 gacttatctc aagataaaat ggcattacaa cgtttgaaag atgctgctga aaaagctaaa
     9121 aaagacttat caggtgtatc acaaactcaa atctcattac catttatctc agctggtgaa
     9181 aacggtccat tacacttaga agtaaactta actcgttcta aatttgagga attatcagat
     9241 tcattaatta gaagaacaat ggaacctaca cgccaagcaa tgaaagacgc tggcttaaca
     9301 aactcagata tcgatgaagt tatcttagtt ggtggatcaa ctcgtattcc agcggtacaa
     9361 gaagctgtca aaaaagaaat cggtaaagaa cctaacaaag gtgtaaaccc ggacgaagta
     9421 gtggctatgg gagctgcaat ccaaggtggc gttatcacag gtgacgttaa agacgtagta
     9481 ttattagacg taacaccact atctttaggt attgaaattt taggtggacg tatgaatacg
     9541 ttaattgaac gtaacactac gattcctaca tctaaatctc aaatctattc aacagcagta
     9601 gataatcaac catcagtaga tgtacacgta ttacaaggtg aacgtccaat ggctgcggat
     9661 aataaaacac ttggtagatt ccaattgact gatattccac cagctgaacg tggtaaacct
     9721 caaattgaag taacgtttga tatcgataaa aacggtattg taaatgtaac tgcaaaagac
     9781 ttaggtacaa ataaagaaca aagaattaca attcaatcaa gttcttcatt atcagacgaa
     9841 gaaatcgacc gtatggtaaa agatgctgaa gttaacgctg aagcagataa aaaacgtcgt
     9901 gaagaagtag acttaagaaa cgaagctgac agcctagtat tccaagttga aaaaacttta
     9961 actgatttag gcgaaaatat cggtgaagaa gataaaaaat ctgctgaaga gaaaaaagac
    10021 gctcttaaaa ctgctttaga aggtcaagat atagaagata ttaaatctaa aaaagaagaa
    10081 cttgaaaaag tgattcaaga attatcagca aaagtatatg agcaagcggc tcaacagcaa
    10141 caacaagcac aaggtgctaa tgctggtcaa aacaacgata gtactgtaga agatgctgaa
    10201 tttaaagaag taaaagacga cgacaaaaaa taattaaaca atacaattag aatgttgtca
    10261 tgatctgaaa agtcaaagtc aatagaacat tggctttggc tttttatttg gatagtatga
    10321 catattacgt taaactatat gagttaatta agaaaaggag agatagctgt ggccaaaaga
    10381 gactattatg aagttttagg cattagtaaa gatgcttcaa aagatgaaat caaaaaagcg
    10441 tatcgaaagc tttcgaaaaa atatcatcca gatattaaca aagaagaagg tgcagatgaa
    10501 aagtttaaag aaatttctga agcctatgaa gttttaagtg atgataataa acgcgcaagc
    10561 tatgatcaat ttggacatga cgggccacaa ggttttggtg gacaaggatt caatggctct
    10621 gactttggcg gttttagtgg cttcggtggc ggcggttttg aagatatctt tagctctttc
    10681 ttcggtggcg gcagacaaag agatccaaat gcaccgcaaa aaggtgatga tcttcagtac
    10741 acaatgacat tgacatttga agaagcggta tttggtacaa caaaagaaat ttcaatccgt
    10801 aaagatgtaa catgcgaaac atgtcatggt gatggtgcaa agcctggaac aagtaaaaag
    10861 acttgtagtt actgtaatgg cgctggtcat gtagctgtag aacaaaatac aattttaggt
    10921 agagttcgca ctgaacaagt ttgtcctaaa tgtaatggaa gtggtcaaga atttgaagaa
    10981 gcttgtccaa catgtcacgg aaaaggtact gaaaataaaa cggttaaatt agaagtgaaa
    11041 gtacctgaag gtgtcgacaa tgaacaacaa attagattag ctggtgaagg ctctccaggc
    11101 gtgaatggag gacctgctgg tgatttatac gtagtattta gagttaaacc atctgaaaca
    11161 tttaaacgtg atggtgatga tatttattac aaattaaatg ttagcttccc acaagcagct
    11221 ttaggcgatg aaataaaaat accaacatta aataatgaag ttatgttaac aatcccagct
    11281 ggtacacaaa caggtaagca attccgctta aaagaaaagg gtataaaaaa tgttcatggc
    11341 tatggatacg gagatttata tgttgatatt aaagtagtaa cacctactaa attaacagat
    11401 agacaaaaag aactaatgaa agaatttgcc caattaaatg gtgaagaaat taatgatcaa
    11461 ccttcgaatt ttaaagatag agcaaaaaga ttctttaagg gagaataaag tatgaactgg
    11521 acagagcttt caattattat taatcatgaa gcagtagaat tggctaccaa tatacttgaa
    11581 aatcatggat caaatggtgt cgtgatagaa gattcatatg atttaattaa tcaaccagaa
    11641 gataaatacg gtgaaattta cgctttgaaa aaagaggatt atccagataa gggagtaaga
    11701 ttgaaagcct attttaatga gttgacttat gatgataagt tgcgacagcg aattaaagat
    11761 gagttattaa acttagatga acttgatcaa cataacgttc aattcagtga gcaaatcatt
    11821 gcagaaacgg attgggaaaa tgaatggaaa aactatttcc atccattccg agcgtcgaag
    11881 aagttcacaa tagtccctag ttgggaaaca tatgctaaag aagcggatga agagctttgc
    11941 attgagctcg atccaggtat ggcttttgga acaggagatc atccgactac aagtatgtgt
    12001 ttgaaggcaa tagaaacata tgtattgcca cagcattcag taattgatgt tggtactggc
    12061 tcaggtatat taagtattgc aagttatcta atcggtgtaa aacgtattaa agcgttggat
    12121 attgatgaaa tggcagtgag tgtagctaaa gaaaacttca gaagaaatca ttgtgaaacg
    12181 ttaattgaag ctgttccagg taacttattg aaagacgaaa cagaaaaatt tgatattgta
    12241 atagcaaata ttttagcgca tattattgat gaaatgattg atgatgctta taatactcta
    12301 aatgaaggcg gttattttat tacttctggt attataaaag agaagtatga aggtatacag
    12361 tcacatatgg agcgtgtagg ttttaaaatt atttcagaac aacatgacaa cggttgggtt
    12421 tgtcttgttg gccagaaagt gagtgaataa tgtgcaacgt tatttcatag accaaaacgc
    12481 tgatgtaagt cagcgttttt ttattacaaa aaaagaagat attcatcata taacaaatgt
    12541 aatgagatat gatgttggta gtaaaattat tttaactttt aatgatcaaa cagtttattt
    12601 atgtgaaatt gctgatataa caagtgataa aattgcagtt gatttaaaag aaaaacaaaa
    12661 tattaataca gaattgccag ttgatgttac gatttgcagt ggactaatta aagctgacaa
    12721 atatgagtgg ttgctacaaa aagctactga attgggtgct tcatcgttta tagctgttag
    12781 catggaacgt tcaattgtta aattaaacga ggcaaaagtt gccaaaaaga tagaacgttg
    12841 gcaaaaaata attaaagaag ctgctgaaca aagctatcgt ttagtgattc catctattca
    12901 attcgagtcg aatttaaaat taatttgtga tactattgat aattatgact atattcttat
    12961 tgcacatgaa gaggaagcaa aagatggtga gttaagcaat ttcaagcaaa ctttacaaca
    13021 attcaaggct caggataaag tgttgatgat ttttggtcct gagggtggtt tgtcagaaaa
    13081 tgaaatttca ttatttagta atactagtac agttgttggt cttggaccga gaattttacg
    13141 ggcagaaact gcgccattgt atgcgttaag tgcaataagt tatgaaaaag aattaatggg
    13201 gtgaatattt atgtcaacag ttgcgtttca cacattaggt tgtaaagtaa accattatga
    13261 aactgaagca atctggcaat tatttaaaga agcaaactat gagcgcgttg actttgaagc
    13321 gaatgctgat gtatttgtta ttaatacttg tacagtaacg aatacaggtg ataaaaaaag
    13381 tcgtcaaatt attagacgtg caataagaca aaatcctgat gctgtaatct gtgtaacagg
    13441 ttgttatgcg caaacttcat cagctgaaat tatggaaatt cctggtgtcg atgtagtagt
    13501 tggtacacaa gatagacata aactattagg ttacattgat gaattccgta aagaacgcca
    13561 accaattaat ggtgttggaa atatcatgaa aaatcgtaaa tatgaagaat tagatgtccc
    13621 ttattttaca gatagaacac gtgcgtcatt aaaaattcaa gaaggttgta acaacttttg
    13681 cacattctgt attattccat gggctcgtgg cttaatgcgt tcaagagatc cggaaaaagt
    13741 agttgaacaa gcgacgcaac tagtgaattc aggatataag gaaattgtat tgacgggaat
    13801 tcatacaggt ggatatggtc aagatttaaa agattataac ttggcccaat tattacgtga
    13861 tcttgaaacg attaatggat tagaacgaat tcgaatttct tcaattgaag caagtcaact
    13921 tacagatgaa gtgattgacg ttttagaacg ttcaacaaaa gttgtgcgtc atttgcatat
    13981 tccgttacaa tctggttcag atacagtatt aaaacgtatg agacgtaagt atacaatgga
    14041 taggttttcg gaacgattaa caaaattgca taaagcttta ccagacttgg cagttacgag
    14101 tgatgtaatt gttggtttcc caggtgaaac tgaagaagag ttccaagaaa catatgattt
    14161 tatcgtaaag cataagttct ctgaactgca tgttttccct tattctccta gaattggcac
    14221 gccagctgca agaatggatg accaaattga tgaagaaatt aaaaatgaac gcgtgcataa
    14281 gttaattacg cttagcaatc aacttggaaa gttatatgcg tctaaatttg atcaagatgt
    14341 gcttgaagta attcctgagg agcagggtga cacagaaggt acattagttg gatatgcaga
    14401 taattatatg aaagtacaat ttgaaggtga cgaatcactc ataggtcaaa ttgtaaaagt
    14461 taaaattacg caagcaaatt atccattaaa tgaagggcaa gcaattaaag ttgttgattt
    14521 cgcaacaaat aaatctgata gagaagtttt agtttaagta ttccttgatt cataccataa
    14581 ggttccgtat taaagatata ggttttgtaa cgttgtttta acagcttcac aatttactat
    14641 ataaacaact cttctcatta aataaattat taagagattt ttagaacaaa atctaaaagt
    14701 ctttgtgatt ttgatgtgaa atatgtttca aataaaaaac aatttactaa ttgaccataa
    14761 attacagata tattatactt ataaatgcat agttttactg tgcaattgac tataaagttc
    14821 cgttgatatt tggagggagg gaaatacaga tgtctaaaac agtagtacgt aaaaatgaat
    14881 cacttgaaga tgcgttacgt agatttaaac gttcagtttc taaaagtgga acaatccaag
    14941 aagtacgtaa acgtgaattt tacgaaaaac caagcgtaaa acgtaaaaag aaatcagaag
    15001 ctgcacgtaa acgtaaattc aaataattaa tacctctgtt gactccctca acacgaatat
    15061 taattatata aaataaacat cacaagttag tgtctgacac taatatgtga tgtttttttg
    15121 ttgtcaattt ttaatttaaa aaaattatat agtttataaa taatcaaatt gatattctat
    15181 aggttcttat aactataaag tatattcaat ttcatgtata attaatgtga gggcgaggtg
    15241 aaattgtgag ttataataat tttttacaaa tgacaactat cttggaatca acggctggag
    15301 atacttgggt tgaacaagtt agtaatataa ttgttcaacc tatttttacg ttaatattaa
    15361 cctgtttgac attcttagga tttgtatatc aactttactc taaaaaaatc aatgcagctg
    15421 gtattatcgc cacattatca ttacttattt tatttttggg atttctaatc caaggaaatg
    15481 tcaatatgca ttctatctta atattctcaa ttggcgttat attagttgta attgaattat
    15541 ttgtagttgg tgcagtaatt ggtattattg gcatgatact gataactata agcataacaa
    15601 cgctcggtga taatttgcta tttatgcttg cgaatgttat cgttgccttg attttaacga
    15661 ttgtagaatg ggtgatatta gtgaagattt tcaacagaaa gattccgttt ttggataaag
    15721 ttatcttaaa agattcaact aattctgagt caggttacaa ttctcatgat aaccgctcgc
    15781 acctcgtagg aaagactgct caaacagtta cagatcttcg acctgcaggg attatttttt
    15841 gtgaaaatga acgtattgat gctgtttcag atggcaactt tattttacgc aataaaacgg
    15901 taaaaatcct tgaagttgaa ggaacaagag tagttgtgag ggaagtagat taattaaaag
    15961 gagcgatacc atgtttagtt taagttttat cgtaatagca gttattatag tagttgcatt
    16021 acttatttta ttctcatttg taccgattgg tttatggatt tcagcgttag cagctggcgt
    16081 tcatgttggt ataggtacat tggttggtat gcgtttacgt cgtgtatctc caagaaaagt
    16141 tatagcgcca ttaattaaag cgcataaagc aggactagca ttaacaacaa accaattaga
    16201 atcgcattat ctagcaggag gaaatgttga cagagttgtt gacgctaata ttgctgcaca
    16261 acgtgctgac attgaccttc ctttcgaacg tgcagctgct attgacctag caggacgtga
    16321 cgtattagaa gcggttcaaa tgtctgttaa tcctaaagtc attgaaactc catttatcgc
    16381 aggtgtagca atgaatggta ttgaagtgaa agccaaagct cgtatcacag ttagagctaa
    16441 tattgctcga cttgttggtg gtgctggtga agaaacaatc atcgcacgtg ttggtgaagg
    16501 tatcgtttca acaattggtt ctagtaagca tcatacagaa gtacttgaaa acccagataa
    16561 tatttctaaa acagtattaa gcaaaggttt agattcaggt actgcatttg aaattttatc
    16621 aattgatatt gctgacgttg atattagtaa aaatattggt gcagacttac aaactgaaca
    16681 agcattagca gacaaaaata ttgcacaagc aaaagctgaa gaacgtagag ctatggctgt
    16741 agcaactgag caagaaatga aagcgcgtgt acaagaaatg catgctaaag tagttgaagc
    16801 cgaatctgaa gtaccattag ctatggctga agcattacgt tcaggtaata tcagtgttaa
    16861 agattattat aatttgaaaa atatcgaagc tgatacaggc atgagaaatg caattaataa
    16921 acgaactgat caaagtgatg atgagtcacc tgaacattaa gtcgagaggt gattaaatga
    16981 gtgtcggtat tctaattttt gtcatatcag tgatcatttc tatcattact actatgcgcg
    17041 aaaatagtca taaagataga caaaatcaaa agccacctca aaaaacatct accgataatg
    17101 aaccaaaaaa aggtggcttt tttgaagaaa ttgagcgaac gtttaaagaa ataagtgaag
    17161 aattaaatga agaagaaaag aaatcatcga aacgaaaata cgatgatacg ttaccacctt
    17221 tattcgatga acttccaaag gaagagccta aatcgaaacc tgttgtagaa cctatggcac
    17281 ctaaaaaaca acaagaaaca aaaccgatga cagagaaacc aattacagtg cctaaagcag
    17341 aaccggtgga gcagaaacat agaccttcta gacaagataa ttctgacgaa attagacgtc
    17401 aattagaaaa atcacttaga gatgatatta aaacgattcg tactgacatt gatagagaaa
    17461 aagaaaagca aattgctaaa atggaaaaac gtgctagaga tattattgag gataaatact
    17521 tatctgaacg tacaaaacgt ttgaaattaa agcagttgct taattctcaa aatgtcgaaa
    17581 aagatttgac taaatcagcg ttccaatttg ataaagatga agtaatcaat ggtatgatat
    17641 ggtcagaaat tttagctaaa ccaaaacaat tataaaattt ttgaaaacaa gcactatcgt
    17701 aatggtagtt gcttgttttt ttacgttaag aaaaattgaa aatcaaagag aatctttcga
    17761 gaaatatgag ttgtttaaat tacaacaaaa aattgattag tctaaaattg aatctgtttt
    17821 tatgacaagg tgaaaagtat aaatgattat tttaaattaa agaaaaatca gtaagtcaat
    17881 gcaaagatgt ttaaatcaat caattgcatg atataattaa gtagatatta aagcatcata
    17941 gaatgaatat aaatgatata tgaaaaggag cgcgtgtatg cctggaatta tacaaataga
    18001 cgatatgaac caatctcaag ctttaattgg aaataatgat gaacatttaa aagcaattga
    18061 agagagtttc gatgttgtca tccatgcaag aggacaagaa gttgccgtta aaggtacaaa
    18121 aatagaaaac gtagaaaaag cggaatcagt attaatcaat ttgctgaagg ttattgattt
    18181 aggtaataat attacaatta aagatgttga agcagctatt aaaatggcgc ataataacac
    18241 aattcaacat ctgttagatt tatatgatga agagataact aaagatgcat ttggtaagac
    18301 gattcgtgcg aaaacgatgg ggcaacgtat atatgttaat gccatgaaaa ataatgattt
    18361 agtatttggt ataggtcctg ctggtacagg taagacattc ttagctgtag tttatgcagc
    18421 aaagcaactc cgtaaaggta ctgttaaacg tattgtatta acaagacctg ctgttgaagc
    18481 aggagagtca cttggatttt taccaggaga tttgaaagaa aaggtagatc catatttaag
    18541 acctttatat gatggtctat atactgttct tgggcgtgaa caaacagagc gatttattga
    18601 aagaggcatt atcgaaatag cgccacttgc atatatgcgc ggacgaacat tagaagatgc
    18661 atttgtaatt cttgatgagg cgcagaatac gacacatgcg caaatgaaaa tgtttttaac
    18721 aagactaggt tttggctcaa aaatggtagt tactggtgac caaactcaaa tcgatttacc
    18781 taaaggtgtt aaaagtggac ttaaggaagc ggtcagtagg ttacacaacg ttaaaggtat
    18841 aagtatattg aaattagatc agagcgatgt agtaagacat ccattggtaa gtaaaatcat
    18901 tgaacattat gaaggagaga attaaatgtt tacgatagat tttagcgatc acacaggctt
    18961 ag
//
LOCUS       DAMZPP010000043        18925 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMZPP010000043 DAMZPP010000000
VERSION     DAMZPP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18925
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.043"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..399
                     /gene="ybeY"
                     /locus_tag="POH73_001144"
     CDS             <1..399
                     /gene="ybeY"
                     /locus_tag="POH73_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDI6887110.1"
                     /translation="LLEFAKKEEHIEDDAELSVTFVDKQEIQEINRTYRDKDKVTDVI
                     SFALEEDEPDIDFSGLDIPRVLGDIIICTDVAQEQANNYGHSFERELGFLALHGFLHL
                     LGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            402..746
                     /locus_tag="POH73_001145"
     CDS             402..746
                     /locus_tag="POH73_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDI6887111.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            757..1161
                     /gene="cdd"
                     /locus_tag="POH73_001146"
     CDS             757..1161
                     /gene="cdd"
                     /locus_tag="POH73_001146"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDI6887112.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSHFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            1162..2061
                     /gene="era"
                     /locus_tag="POH73_001147"
     CDS             1162..2061
                     /gene="era"
                     /locus_tag="POH73_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDI6887113.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILDLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            2089..2835
                     /gene="recO"
                     /locus_tag="POH73_001148"
     CDS             2089..2835
                     /gene="recO"
                     /locus_tag="POH73_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDI6887114.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNKWRGMGTLNSVDVISQHYKLQMDLYVSSYASLAAETIERSMDE
                     GDVAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKYDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(2986..4377)
                     /locus_tag="POH73_001149"
     CDS             complement(2986..4377)
                     /locus_tag="POH73_001149"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HDI6887115.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            4712..5335
                     /locus_tag="POH73_001150"
     CDS             4712..5335
                     /locus_tag="POH73_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6887116.1"
                     /translation="MELSQRQERIIEIVKTQGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHTVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            5346..6164
                     /locus_tag="POH73_001151"
     CDS             5346..6164
                     /locus_tag="POH73_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDI6887117.1"
                     /translation="MEKNKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            6225..8024
                     /locus_tag="POH73_001152"
     CDS             6225..8024
                     /locus_tag="POH73_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDI6887118.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPE
                     PIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDF
                     YAENDQYNISDAVQYVNSNELRETLISLEQYSLNDEPYENEIDDYVNVINENGQETIE
                     SLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            8248..9354
                     /gene="rpoD"
                     /locus_tag="POH73_001153"
     CDS             8248..9354
                     /gene="rpoD"
                     /locus_tag="POH73_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDI6887119.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            9485..10162
                     /locus_tag="POH73_001154"
     CDS             9485..10162
                     /locus_tag="POH73_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDI6887120.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIHPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     QLIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHRQRLAQINDEI
                     AVIERVL"
     gene            10165..11265
                     /locus_tag="POH73_001155"
     CDS             10165..11265
                     /locus_tag="POH73_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDI6887121.1"
                     /translation="MKIADLMTLLDHHVPFRTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPHGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDACQKSWAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAANIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKTLLMNWFNTEKINIDVEASTINTDPFEYI"
     gene            11379..12725
                     /locus_tag="POH73_001156"
     CDS             11379..12725
                     /locus_tag="POH73_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6887122.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPEQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFFLIPTKGAAKVEKTLNLIDILNPYLCIVFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            12735..13625
                     /locus_tag="POH73_001157"
     CDS             12735..13625
                     /locus_tag="POH73_001157"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDI6887123.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQHFDPELKNKVMQQ"
     gene            13751..14536
                     /locus_tag="POH73_001158"
     CDS             13751..14536
                     /locus_tag="POH73_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDI6887124.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            14578..15441
                     /locus_tag="POH73_001159"
     CDS             14578..15441
                     /locus_tag="POH73_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDI6887125.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            15428..15838
                     /locus_tag="POH73_001160"
     CDS             15428..15838
                     /locus_tag="POH73_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDI6887126.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            16114..16713
                     /locus_tag="POH73_001161"
     CDS             16114..16713
                     /locus_tag="POH73_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDI6887127.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            16833..18908
                     /locus_tag="POH73_001162"
     CDS             16833..18908
                     /locus_tag="POH73_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDI6887128.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIVFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVSINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETKGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDDVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     DDKNKDKDK"
BASE COUNT         6949 a         2579 c         3487 g         5910 t
ORIGIN      
        1 ttattagaat ttgctaaaaa agaagagcat atagaagacg atgctgagct ttctgttaca
       61 tttgtagata aacaagaaat acaagaaatt aatcgaacat atagagataa agataaagtt
      121 acagatgtaa tctcatttgc tttagaagag gatgagccag atattgattt tagtggtctt
      181 gatataccac gtgttttagg ggatataatt atctgtacgg atgtagcgca agaacaagca
      241 aacaattacg gacattcttt tgaacgagaa ttaggatttt tagcattaca tggatttttg
      301 catctattag gttatgatca tatgactgaa gcggatgaaa aggaaatgtt tggtcgacaa
      361 gatacaatat taaacgcata tggattaaca cgagactaat tatgaaaagg tttaaatatg
      421 cacttgatgg gctgaaaatc ttaattcaaa aagactataa atttctttta catgtgtttg
      481 caatgattgt tgctattgtc tttggtctcg tactaaatat taatcggatt gagtggatat
      541 ttatactcat tgctattgca ttagttctca ctgttgaagc tttaaacact gctattgaat
      601 atgttgtaga tttagtgacc gttgaatatc atgatttagc taaatacgct aaagatatag
      661 cggcttttag tgtacttata gtttcaatat tagcatttat tataggttta atagtatttt
      721 taccacattt tatagcgtta ttttagggag gcatatatga gttatcaacc tcattatttt
      781 caagaagtta gaaaagcaca acaagaatca tattcgccat acagtcattt taaagtaggg
      841 gcttatttaa aaacgaaaga cggtagaact ttttatggta ccaatgtaga aaatgcttct
      901 tatccattat cgatatgtgc tgaacgagct agtttggtat cggcaatttc tcaaggatac
      961 agaccaggtg attttgaatc aataactgta accgtagatg cagataaacc gtcatcacct
     1021 tgtggtgcat gtcgtcaagt tttgaaggaa ttatgtgatg atgatatgcc tgtgtatatg
     1081 acaaatcata aaggagatat ggttatgatg acagtcgcag agttactacc atttggattt
     1141 tcaggaaagg atttagaata aatgacagaa cataaatcag gatttgtttc aattataggt
     1201 agaccaaatg tagggaagtc aacatttgtt aatagagtga ttggccataa aatagcaatc
     1261 atgtccgata aagctcaaac aactagaaat aaaattcaag gtgtaatgac aagagatgat
     1321 gcgcaaatta tattcattga tacgccaggt attcataaac ctaaacacaa attaggtgac
     1381 tatatgatga aagtcgctaa aaatacatta tctgagatag atgcaatcat gtttatggtt
     1441 aatgccaatg aggaaattgg acgaggcgat gaatatatta tagaaatgtt gaaaaatgtt
     1501 aagacaccag tatttttagt attaaataaa atagatttag tgcatccaga tgaattaatg
     1561 ccaaagattg aagaatatca aagttatatg gactttacag agattgtacc tatttcagca
     1621 ttagaagggc taaatgtaga tcattttatt gatgtattaa agacgtattt acccgaagga
     1681 ccaaaatatt atccagatga tcaaatttca gaccatcctg aacaatttgt agtgggtgaa
     1741 atcattcgtg aaaaaatcct tgatcttaca agtgaagaaa tccctcatgc gattggtgtt
     1801 aatgtggacc gtatggttaa agaaagcgaa gatcgtgttc atatcgaagc aactatatat
     1861 gttgaaagag attcgcaaaa aggaattgtc attggaaaag gcggtaaaaa gctaaaagaa
     1921 gtaggaaaac gtgcgagacg tgatatagaa atgcttctag gctctaaagt ttacttggaa
     1981 ttatgggtca aagttcaaag agactggcga aacaaagtta actttattcg ccaaattggt
     2041 tatgttgaag accaagatta atcttaaaag tggtgaagat aattgttaat gcgccaaaaa
     2101 gggattatca tcaaagcagt tgattatggt gaatctgata aaattatcac gattttaaat
     2161 gagcatggtg caaaagtacc acttatggca aggcgtgcta aaaaagttaa gacgggtttg
     2221 caagcgcaaa cgcaattgtt tgtttatggt ttgtttattt acaataagtg gcgaggtatg
     2281 ggaacgttaa attctgtaga tgttattagt caacattata aattacaaat ggacctttac
     2341 gtaagcagtt atgcgtctct ggcagctgaa actattgagc gttcaatgga tgaaggggac
     2401 gttgcaccat ataactatca attattacaa tttgttcttg aaaaaataga atcaggtaca
     2461 tctgcacagt taatgtcagt cgttgttatg ttaaagtgta tgaaacgatt tggttttacc
     2521 gcatcattta atcgctgtgc tgtgagtggt aatgacacac aagcagattt aataggttat
     2581 agttttaagt atgacggtgc tatttcaagg caagaggctt ctaaagatgt acatgcagtt
     2641 atattatcga ataaaacact atatttatta gatgtattac aaaaattacc aatagataaa
     2701 atgaattcat tgaatatcca tcaagaaatt attgatgaaa tgtcagatat cattttaatg
     2761 ttatatcgtg aatatgcagg tatgtttttt aaaagtcaga aactaatcaa ccaattaaaa
     2821 agattggaac aataacataa aataataaaa ggtattcaag tagccacata gatgtgttta
     2881 tttgaatacc tttttcgaat agaaaagaga tactggcaat tttactaacc agtatctctt
     2941 tttataaatt atatagccac cacatatggt ggagagtctt tttaattaga attttgtttt
     3001 ttcagttaag aaagcttcta actctgagat tggcatacga acttgttcca ttgaatctct
     3061 gtcacgtact gtaacttgat tatcttctaa tgaatcaaag tcgaatgtta cacaataagg
     3121 tgtaccaatt tcatcttgac gacgatatct tttaccgata gattgtgatt catcgaaatc
     3181 gattgagaat ttagaactta attgctcaaa aatcttaatc gcttcgccag ataatttctt
     3241 gcttaaaggt aaaatcgctg ctttatatgg tgctaatgca ggatggaagt gtaaaactgt
     3301 acgtgcatct ttactacctt caacgccttc ttcatcatat gcatcacata agaaagctaa
     3361 tgttacacga tctgcaccaa gtgatggctc gatacaatat ggaatatatt tttcgttcgt
     3421 ttctggatcg tggtatctga aatcttcacc agagtgttca gcatgtttac gtaagtcgaa
     3481 gtctgtacga cttgcgatac cccataactc accccaacca aatgggaatt tatattcaat
     3541 atcagttgtt gcatttgagt aatgagataa ttcatcttca tcatgatcac gtaaacgcat
     3601 attttcacta ctcatattta agcttgttaa ccagtcactt gcaaaagttt tccaataatt
     3661 ttgccattcg atttcttcac caggtttaca gaagaattca agttccattt gttcaaattc
     3721 tcttgttctg aaaatgaagt tacctggagt gatttcatta cggaatgatt taccaatttg
     3781 accgatacca aatggtaatt ttttacgcat tgaacgttgc acgtttttat agtttacaaa
     3841 aataccttgt gctgtttcag gacgtaagaa aatttcattt gtagaatctt cagttacacc
     3901 ttggaatgtt ttaaacatta aattgaattg gcgaatttca gtccagttag cagttttact
     3961 tacaggacaa acaatacctt catcgtcaat aatttttttc atttgttcaa aacttaaacc
     4021 atcggcaatg aagttttcat cacctttaac atcttgcata taatcttcaa ttaatttatc
     4081 agcgcgatat cgaattttac tatctttatt atcaatcatt gggtcgttga agttgtttaa
     4141 gtgtcctgaa gcttcccata cttttggatt cattaagatt gcagcatcga taccaacgtt
     4201 aaacggtgat tgtgtaatga atttttgcca ccaagctttt ttaacattat tctttaattc
     4261 aacacctaaa ggaccataat cccatgtgtt tgataaacca ccgtaaatat cactaccagg
     4321 gaacacaaaa cctctgtgtt ttgctaatga aacaattgta tccatatctt ttgccataat
     4381 tacctctcct catacatgaa aacgccccat ggaaaataac agcatagtaa atatgcttta
     4441 cttccatggg acgagttaat attttaaatt gtatataata caaaataagt tacgtattta
     4501 acccgcggtt ccacccaaat tagtgtagtc actcgctttt attttaaaat gattcgttgc
     4561 gccaatctta ttgttaagct tacactatcc ttaactcgct gtacgtttat tctaattgat
     4621 agattattta aaggcaagta aaattaatgt gtttgtgaca tgaaagaaat atcggtataa
     4681 taataagtga ataaaaaagg ggtgaagacc tatagaactc agtcaaagac aagaacgaat
     4741 catcgaaatt gttaaaactc aaggacccat tactggtgaa caaatagcag ataagttgaa
     4801 tttaacaaga gcaacgctta gaccagattt agcgatatta acaatgtcag gttttttaga
     4861 agcgcgtccc cgagttggat attattattc aggtaaatca aaaggtaaat tttttaatga
     4921 aaaacttcga caatttgagg ttaaggacta tatgtctcaa cctgttgtgc taagagagaa
     4981 tacaacagtt tatgacgcta tttgtacaat ttttttagaa gatgtaagta cattatttat
     5041 tattaatgaa gataacgatt ttgttggtgt gtgttcaaga aaagatttat taagagcttc
     5101 aatgattgga gcagatattc atacagtacc tatcagtgtt aatatgacac gtatgcctaa
     5161 tgtcacttat ttagaggaaa gcgaattagt catatacgca gcagatagaa tgattgaaaa
     5221 agaaattgat tcgattccaa ttgtaagaaa aaaagataat caaaagtatg aagtaattgg
     5281 aagaatttcc aaaacaacaa tagctaagtt attagtagca ttatataaag aataggtgag
     5341 aagtaatgga aaaaaataaa attatcgtag cttcagattc tataggtgaa acggcagagt
     5401 tagttgctag ggcaggtatt tcacaattta atcctaagca atgtaaaaat gaattattaa
     5461 gatatccata tattgaatct tttgaagatg ttgatgaagt gattcaagtt gctaaagata
     5521 caaatgctat cattgtttat acacttatta aacctgaaat gaagcaatat atgagtgaga
     5581 aagtagcaga attccaattg aagtccgtcg atattatggg gccattaatg gatttattat
     5641 cagcatcagt tgaagaaaaa ccttataatg agccaggtat cgttcataga ttagatgatg
     5701 catatttcaa gaaaattgat gcgatagagt ttgcagttaa atatgatgat ggtaaagatc
     5761 ctaaaggatt acctaaagct gatattgttt tacttggtat ttcgagaact tcaaagacac
     5821 cattatccca gtatttagcg cataaaagtt acaaagttat gaatgtaccg attgtaccag
     5881 aagtgacacc gccagatggc ttatatgata ttgatccaaa gaaatgtatc gcacttaaaa
     5941 taagtgaaga aaaattaaat cgcattagaa aagagcgact aaaacaatta ggactaggtg
     6001 acacagctcg atatgcaaca gaagcacgaa ttcaagaaga attgaattac tttgaagaaa
     6061 tcgtaagtga aattggatgt cctgtcattg atgtttctca aaaagcaatc gaagaaacag
     6121 caaacgatat aatccattat attgaacaaa ataaatcgaa atgatttcat ttttgtcaaa
     6181 aattaggtat aatagtataa ctaatgctta ataggtgatt taatttgcga atagatcaat
     6241 cgatcattaa tgaaataaaa gataaaaccg atattttaga cttggtaagt gaatatgtaa
     6301 aattagaaaa gagaggacgc aattatatag gtttgtgtcc ttttcatgat gaaaagacac
     6361 cttcatttac agtttctgaa gataaacaaa tttgccattg ttttggttgt aaaaaaggtg
     6421 gcaatgtttt tcaatttact caagaaatta aagacatatc atttgttgaa gcggttaaag
     6481 aattaggtga tagagttaat gttgctgtag atattgaggc aacacaatct aactcaaatg
     6541 ttcaaattgc ttctgatgat ttacaaatga ttgaaatgca tgagttaata caagaatttt
     6601 actattacgc tttaacaaag acagtcgaag gcgaacaagc attaacgtac ttacaagaac
     6661 gtggttttac agatgcgctt attaaagagc gaggcattgg ctttgcaccc gatagctcac
     6721 atttttgtca tgattttctt caaaaaaagg gttacgatat tgaattagca tatgaagccg
     6781 gattattatc acgtaacgaa gaaaacttca gttattacga tagatttcga aatcgtatta
     6841 tgtttccttt gaaaaatgcg caaggaagaa ttgttggata ttcaggtcga acatataccg
     6901 gtcaagaacc aaaataccta aatagtcctg aaacacctat ctttcaaaaa agaaagttgt
     6961 tatataactt agataaagca cgtaaatcaa ttagaaaatt agatgaaatt gtattactag
     7021 aaggttttat ggatgttata aaatctgata ctgctggctt gaaaaacgtt gttgcaacaa
     7081 tgggtacaca gttgtcagat gaacatatta cctttatacg aaagttaaca tcaaatataa
     7141 cattaatgtt tgatggggat tttgcgggta gtgaagcaac acttaaaaca ggtcaacatt
     7201 tgttacagca agggctaaat gtatttgtta tacaattgcc atctggcatg gatcctgatg
     7261 aatacattgg taagtatggc aacgacgcat ttactgcttt tgtaaaaaat gacaaaaagt
     7321 catttgcaca ttataaagtg agtatattaa aagatgaaat tgcacataat gacctttcat
     7381 atgaacgtta tttgaaagaa ctgagtcatg acatttcact tatgaagtca tcgattttgc
     7441 aacaaaaggc tttaaatgat gttgcaccat ttttcaatgt tagtcctgag caattagcaa
     7501 acgaaataca attcaatcaa gcaccagcca attattatcc agaagatgag tatggcggtt
     7561 acattgaacc tgagccaatt ggtatggcac aatttgacaa tttgagccgt caagaaaaag
     7621 cggagcgagc atttttaaaa catttaatga gagataaaga tacattttta aattattatg
     7681 aaagtgttga taaggataac ttcacaaatc agcattttaa atatgtattc gaagtcttac
     7741 atgattttta tgcggaaaat gatcaatata atatcagtga tgctgtgcag tatgttaatt
     7801 caaatgagtt gagagaaaca ctaattagct tagaacaata tagtttgaat gatgaaccat
     7861 atgaaaatga aattgatgat tatgtcaatg taattaatga aaacggacaa gaaacaattg
     7921 agtcattgaa ccataaatta agggaagcta caaggattgg cgatgtagaa ttacagaaat
     7981 actatttaca gcaaattgtt gctaagaata aagaacgcat gtagcatgtg gttttaaaga
     8041 ataatacgaa taatgattat gtcaaaatgt ataagggtaa atgatagtta ccgcatttaa
     8101 acaacactat tgaaaaataa atattgggat tagttcctat ttgtaaaata aaattaaaaa
     8161 tatggatgaa ttaattaaga atttagttta aaatagcaat attgaataaa tttcgaatgt
     8221 tcatatttaa aatcgggagg ccgtttcatg tctgataaca cagttaaaat taaaaaacaa
     8281 acaattgatc cgacattaac attagaagat gttaagaagc aattaattga aaaaggtaaa
     8341 aaagagggtc atttaagtca tgaagaaatt gctgaaaaac ttcagaattt cgatatcgac
     8401 tctgatcaaa tggatgattt ctttgatcaa ttaaatgata atgatatttc actagttaat
     8461 gaaaaagata gttcagatac tgacgagaaa ctgaatccaa gtgatcttag tgcccctcca
     8521 ggtgttaaaa taaatgaccc agttcgtatg taccttaaag aaattgggcg tgttaactta
     8581 ttaagtgcac aagaagaaat cgaattagcc aaacgtattg aacaaggtga tgaagtagca
     8641 aaatcaagac ttgcagaagc gaacttacgt ttagttgtaa gtattgctaa aagatacgta
     8701 ggtcgtggta tgttattcct tgatttaatc caagaaggta atatgggtct tattaaagct
     8761 gttgaaaaat ttgactttaa caaaggattt aagttttcaa catatgcaac atggtggatt
     8821 agacaagcaa tcactcgtgc aattgctgac caagcacgta caattcgtat ccctgtgcat
     8881 atggtagaaa caattaataa attaattcgt gttcaacgtc aattattaca ggacttaggt
     8941 cgagatccag caccagaaga aattggtgaa gaaatggatt taccagcaga aaaagttcgt
     9001 gaaattttaa aaattgcgca agaacctgtt tcattagaaa caccaattgg tgaagaagat
     9061 gatagtcatt taggggactt tattgaggat caggaagcac aaagtccttc agatcatgct
     9121 gcttatgaat tattaaaaga gcaattagaa gatgtgcttg atacattaac tgatagagaa
     9181 gaaaatgtat tacgattaag atttggtctt gatgacggca gaacaagaac acttgaagaa
     9241 gttggtaaag ttttcggtgt tacacgtgaa cgtattcgac aaatcgaagc aaaagcactt
     9301 agaaaattaa gacatccaag tcgtagtaaa cgtttgaaag actttatgga ttaatttata
     9361 tcaattttaa tgattgatac caagacatga ataaacgctt atttattttt aaattaagat
     9421 ttaacttgag tgaggttggt acattgaata atgttcaacc tcttttttca taaaggagaa
     9481 taaaatgatt tcgttaaata accgattaac gacggtaagt cgatttttaa aacaaggtac
     9541 aatcgcggat attggctcag atcatgctta tttgccaatc tatgcaatac aaaaccattt
     9601 atgcgaatgc ggtattgctg gagaagttat tcaaggccct tttcaagctg ctgtgaaaaa
     9661 tgtagctgca aaccaattag tagatagaat tgatgtacgt ttaggggatg gcttaagtgt
     9721 tattcatcct gaggatgtaa tcgataatat cacaatttgt ggtatgggcg gaccacttat
     9781 cgctaaaatt ttaaaagatg ggcaagataa gttaagtcaa catccaaggt tgattttaca
     9841 aagtaatatc caaactgaaa atttaagaca aacattacaa caattgaatt atgaaataat
     9901 tgatgaaata ataatggaag aaaaaggcca tatttatgaa attgttgtag cagaatattc
     9961 tacgcaatta atagagctat catctgatga attgaaattc ggaccaaaat tgctgaataa
    10021 caaaaatgaa tattttataa aaaaatggca aagagagtta gaagcacttt atcatattaa
    10081 atcgaaactc aatactgaac aacatcgtca acgtttagct caaattaatg acgaaattgc
    10141 tgtaattgaa agggtgttat agtaatgaaa atagctgatt taatgacatt gttagatcat
    10201 catgtgccat ttagaactgc tgaatcttgg gataatgtag gattgttaat aggtgatgaa
    10261 gatgttgaag ttactggtgt tttaacagca ttagactgta cgttggaagt agtaaatgaa
    10321 gcaatcgaaa aaggttataa tactattatt agtcatcatc ctctaatctt taaaggtgta
    10381 acatcattaa aagctaatgg ttatggtttg attattagaa aactaattca acatgacatt
    10441 aatttaatag cgatgcatac aaatttagat gtaaatcctc atggtgtcaa tatgatgttg
    10501 gcgaaggcga tgggattgaa gaacatttca ataataaata atcaacaaga tgtatactat
    10561 aaagttcaaa catatatacc taaggataat gttggaccat ttaaagataa gcttagtgaa
    10621 aatggattag cacaagaagg taattatgaa tattgtttct ttgaaagtga aggaagaggg
    10681 caattcaaac cagttggtga agctaatcca acaataggac aaattgataa aattgaatat
    10741 gtagatgaag ttaaaattga atttatgata gatgcatgtc aaaagtcatg ggctgagcaa
    10801 ttaattaaac aataccatcc atatgaaaca ccggtatttg attttattga gataaaacaa
    10861 acatctcttt atggacttgg cgttatggca gaagtggata atcaaatgac attggaagat
    10921 ttcgcagcta atattaaatc taaattaaat atcccaagtg tccgttttgt tggtgagtct
    10981 aatcagaaaa ttaaacgtat tgcaattatt ggtggttcag gtattggata tgaatatcaa
    11041 gctgttcaac aaggcgcaga tgtctttgtt acaggtgata ttaaacatca tgatgcctta
    11101 gatgctaaaa ttcatggtgt gaacttaatt gatattaatc attacagcga atatgtgatg
    11161 aaagaaggtt taaaaacgtt actaatgaat tggttcaata cagaaaaaat aaatatagat
    11221 gttgaagcat ctacaattaa tacagatcca tttgaatata tttaaactaa aatctcggga
    11281 tttctaaatt ttgaaatttc gaggttttaa tatttttatt taaaatagca catttatact
    11341 ttataatagt aaagatgaac atataaggag gccaaatcat ggcaaaacat ccattcgaac
    11401 aatttaatct agaatctagt ttaattgacg ctgtgaaaga ccttaatttt gaaaaaccaa
    11461 ctgaaattca gaatcgaatt attccaagaa tactaaagag aacaaattta attggtcaat
    11521 ctcaaacggg tacagggaaa tctcatgcat ttttattacc attaatgcag ttaattgata
    11581 gtgaaataaa agaaccacaa gcaatcgtag ttgcaccaac aagagaactt gcacaacaac
    11641 tatacgatgc agcgaaccat ttaagccaat ttaaagctgg tgtttcagtt aaagttttta
    11701 ttggtggtac agatatagag aaagatagac aacgttgtaa tgcacaacct caattgatta
    11761 taggtacccc tactagaatt aatgatttag ctaaaacggg acatttacat gtgcacttag
    11821 catcatattt agttattgat gaagcggatc ttatgattga cttaggatta attgaagatg
    11881 tagattacat cgctgcaaga ttggaagata atgcaaatat tgcggtgttt agtgctacaa
    11941 ttccagaaca gttacaacca tttttaaata aatatttaag tcatccagaa tatgtagctg
    12001 tcgacagtaa aaaacaaaat aaaaagaaca tcgaattctt tttaatacct actaaaggtg
    12061 cagctaaagt tgaaaagact ttaaatttaa ttgatatact aaatccatac ttatgtattg
    12121 ttttctgtaa tagtagagat aacgcaaatg atttagcacg ttcactaaat gaagctggta
    12181 ttaaagttgg tatgattcat ggtggtttaa cgccacgtga acgtaaacaa caaatgaaac
    12241 gtatacgtaa tttagaattc caatacgtta tagccagcga tttagcatct cgtggtattg
    12301 atattgaagg tgttagtcat gtcatcaatt ttgatgtgcc aaatgatatt gacttcttta
    12361 cacatagagt cggccgaact ggccgtggga attataaagg tgtagcaatt acgctttata
    12421 gtcctgatga agaacacaat atttcattaa tagaagatcg cggttttgtt ttcaatactg
    12481 ttgatattaa agatggtgag ttaaaagaag ttaaagcgca caatcagcgt caagcaagaa
    12541 tgcgcaaaga tgaccattta actaatcaag tgaagaacaa agttcgaagt aaaattaaaa
    12601 acaaagttaa accaggttat aagaagaaat ttaaacaaga agttgaaaaa atgaaacgtc
    12661 aagagcgtaa acaatttagt aagcagcaaa atagacaaaa acgtaagcaa aacaaaaaag
    12721 gttaggtgtg ttttatgtta ttaggatcac atgtttcaat gagtggtaaa aagatgttag
    12781 aaggttctgc tatagaagcg catgaatatg gtgaaacaac atttatgatt tatactggtg
    12841 cacctcaaaa cacgcgccgt aaaagtattg aagatttaaa tataactaaa ggtcatgaag
    12901 tgatggaaaa atatggctta tctaatattg ttgttcatgc accatacatc attaatattg
    12961 caaatacaac caaaccagaa acgtttaatc ttggtgtaga tttcttgcaa caagaaattg
    13021 aaagaacgca agctataggc gctaaagata ttgtattaca tccaggtgca catgttggag
    13081 ctggtgttga tgccggaatt aataagatta ttgaaggatt aaatgaagtt ttaacgaatg
    13141 acaataatgt acgtattgcg cttgaaacga tggcaggtaa aggtacagag attggtcgct
    13201 cgtttgaaga gttagcacgt attattgatg gagttcataa taacgaacgt ttatcagtat
    13261 gttttgatac atgtcatacg catgatgctg gatataatgt gaaagaggac tttgatggcg
    13321 tattaaatga atttgataaa atcattggag tcgacaggat taaagttgtt catgtcaatg
    13381 attctaaaaa tgaccgtggc gctcaaaaag accgacatga aaatattggc ttcggttaca
    13441 ttggttttga tgcattaaat tacattgtgc atcatgatag ttttaaagat attccgaaaa
    13501 tcttagaaac accttatgtt ggtgaagata agaaaaataa aaaaccgcca tataaacttg
    13561 aaattgaaat gttaaaacaa cagcattttg acccagaatt aaaaaataag gttatgcaac
    13621 aataaaatga taatgctctc ttcaagaaat gcgataaata aaacttgaag ggggcatatc
    13681 tctttatttt gtctaatttt gagtcgtaaa cattactgtt tacatatgtt actaggagtt
    13741 gtatagttga atgcgaggtg agaatacgat gacaccagtc tttgaattga aaaatgtcaa
    13801 ttactactat gatcataaaa aagtgttaga aaatataaac attaaaataa ataaaggtga
    13861 atttttagca attgttggac caaatggtgc tggtaaatca acattattga agttgattct
    13921 aggattatta cctttacaaa gtggtgagat ttttgttgaa ggtattgatt ttaaaaataa
    13981 gaaaacatca attaaattaa gctatgtatc acaaaaagca aatgccttta attcaggttt
    14041 cccagcaagt gttaaagaag ttgttttaag cggattaaca aagacaaaac gtcttttcca
    14101 aacatttaat agcaaagata atgaaaaagt gattaaagta ctagaaagac tgaatataag
    14161 tgatttaatt cataaaaata tagcagaatt atcaggtggt caacaacaac gtgtaatgat
    14221 tgctcgagca ttgatttcag aacctgcagt attagtactt gatgaaccga cgaatggtat
    14281 tgatgcaaaa catgtaagtg aattttataa tactttagat caattaaaac aagaaggtat
    14341 cactattatc ttagttactc atgatattgg tgttgtagca gatactgcta ctgaagtagc
    14401 atgtttaaat aagcatttgc atttccatgg tacaactgat gagtttaaat cacttgatga
    14461 agttgaaatt tcaaaaattt atggacatcc tgtacgtttt gtcgatcatc agcataatcg
    14521 agaatgttgt aattaataaa gatttgagtt tatttaaaat ggaaaggcgt gtcacaattg
    14581 atagatgcat tattgaattt tgattttatg aggtactctt taattagtgg tatcttgata
    14641 ggttttattg cgcctctaat cggtgctttt atcgttgtta gacgactatc acttatagct
    14701 gatgctctaa gccatgtaac tttaggtggt atatccttcg gtatgttttt acttactatt
    14761 atgccaacac tagtatttat taatccaatg tggtttggaa tcttattcgc aatagtaggt
    14821 gcgctactaa ttgaaaaatt aagaacgtca tacactgctt accaagaaat tgctattcca
    14881 attataatga gtgctggtat cgccttgagt gcaatcttca tttcattagc tgatgggttc
    14941 aatcaagaaa ttgttggatt gctatttggc tcaataagcg cagtgaatat aagtgattta
    15001 acaacgatta ttgtcattgc catcatagtt gttctgttta ttacattgtt ttataaagaa
    15061 ttatttatat tgtcatttga tgaggaatat agtaaagtca ttggcattcc taaatggatt
    15121 caatttttat ttattgttat tgtagcgatg gttatttctg cctcaatgcg tgttgtaggg
    15181 atactgttag taagtgcgtt aatcacgtta ccaattgcaa taagtatgcg aataacaaaa
    15241 agttttaagc aattgatatt actaagtgta tttttaggtg aattatcagt aatattaggc
    15301 ttagtcctag ctttctatat ggacatttct ccgggtggtg taattgtagt tctactagtt
    15361 attttactta tgattacaat ggcttatcag aaaatgcgaa tgaagtttaa aaagggagct
    15421 aatatcaatg aatacaaatg atgctattaa aattttaaaa gagaacggtt taaaatatac
    15481 agataaacgt aaagatatgt tagatatttt tgtcgaagaa gataagtata taaacgcaaa
    15541 gtatatacaa caagttatgg atgaaaatta tcctggaatt tcattcgaca caatatatag
    15601 aaacctgcac ttatttaaag atttagggat tattgaaaat acagaacttg atggtgaaat
    15661 gaagtttaga attgcttgta caaatcatca tcatcatcat tttatctgtg aaaagtgtgg
    15721 agatacaaag gtaatagatt attgtccaat agatcagata aaattatcac tacctggtgt
    15781 taatattcac aaacacaaac ttgaagttta tggtgtatgt gagtcttgcc aagattaata
    15841 taaagaaatg agatttatgc acatttggtc agatgtatgc ataaatctca ttttttaaat
    15901 tatagaagat aaaggttcat ttcatactcc gttcgtatga ttttatggca aatctgttaa
    15961 ctttttatat acaaaatgtt taattatttt tgatttgagt tatattaaat gagtagagaa
    16021 aataataagt agtagaattt aagcaaattc tttgttgaac cagtcactgc ttgttattat
    16081 ttacttacag acattttagg aggatgatta tttatggctt ttgaattacc aaaattacca
    16141 tacgcatttg atgcattaga accacatttt gacaaagaaa ctatggaaat tcaccatgac
    16201 agacatcata acacttatgt tacgaaatta aatgctgcag tagaaggtac agatttagaa
    16261 tctaaatcta ttgaagaaat tgttgctaat ttagacagtg taccagctaa catccaaact
    16321 gctgtacgta ataatggcgg tggacattta aaccattcat tattctggga gttactttca
    16381 ccaaactcag aagaaaaagg tactgtagta gaaaaaatta aagaacaatg gggttcttta
    16441 gaagaattta aaaaagaatt tgctgacaaa gcagctgcac gctttggttc aggttgggct
    16501 tggttagtcg taaacaatgg ccagttagaa attgtgacta caccaaacca agataatcca
    16561 ttaactgagg gtaaaacacc tattttaggt ttagacgtat gggaacacgc ttattaccta
    16621 aaatatcaaa acaaacgccc tgactacatt ggcgcatttt ggaatgtagt taactgggaa
    16681 aaagttgacg aattatataa tgcaacaaaa taaagtatga ttacttgttc ggtctcttaa
    16741 atgagaccgt tattttttgt caaaaagata gaaataattt ctaaattcat atatgattta
    16801 aagtgaaaga ctttgaatag aggtaggtag ttttgttaaa aagactaaaa gaaaaatcaa
    16861 atgatgaaat cgttcaaaat acaattaaca agagaattaa ctttatattt ggtgtgattg
    16921 tatttatttt tgcagtacta gtactacgtt taggttattt acaaatcgca caaggctcac
    16981 attataaaca aattataaaa aatgatgaaa acattacagt gaatgagtct gtgccaagag
    17041 gtcgtatttt agacagaaat gggaaagttt tagttgataa tgcttctaaa atggctatta
    17101 catatactag gggtcgaaaa acaacacaat cggaaatgtt ggatacggct gaaaagttat
    17161 caaagctaat caagatggat actaagaaga ttacagaacg tgataaaaaa gatttctgga
    17221 ttcagttgca tcctaaaaaa gcaaaagcaa tgatgacaaa agaacaagct atgttagcag
    17281 atggaagtat taaacaagat caatatgata aacaactgtt atcgaaaatc ggaaaatcac
    17341 aattagatga attgtcttct aaagatttac aagttttagc catttttcga gagatgaatg
    17401 caggaacagt tttagatcca caaatgataa aaaatgaaga tgtcagtgaa aaagagtatg
    17461 cagcagtttc tcagcaactt tccaaattac caggtgttaa cacgtctatg gattgggata
    17521 gaaaataccc atatggcgac actttaagag gtatattcgg agatgtatcg acacctgctg
    17581 aaggtattcc caaagaattg acagaacatt acttatccaa aggatattca cgcaatgatc
    17641 gtgttggaaa atcttaccta gaatatcaat atgaagatgt attgcgtggt aagaagaaag
    17701 aaatgaaata cacaacggac aaatctggta aagttacatc ttcagaagtg ttaaatcctg
    17761 gtgctcgcgg tcaagatttg aaattgacga tcgatataga tcttcaaaaa gaagtagaag
    17821 cattattaga taaacaaatt aagaagcttc gcagccaagg tgcaaaagat atggataatg
    17881 ctatgatggt tgtacaaaat cctaaaaatg gagacattct tgcgcttgcc ggaaagcaga
    17941 ttaataagag tggtaaaatg actgattatg acattggtac gtttacttct caatttgcag
    18001 ttggatcttc tgtaaaaggt ggaacattat tagctggtta tcagaataaa gctatcaaag
    18061 ttggagaaac aatggtcgat gaaccattac atttccaagg tggtttgaca aaacgatcat
    18121 actttaataa aaacgggcat gtatctatta atgataagca agctttgatg cattcatcaa
    18181 acgtatatat gtttaaaaca gcattaaaat tagcgggaga cccttattat tctggtatgg
    18241 ctttaccatc agacataagt tcacctgccc aaaagctaag aagaggattg aatcaagtag
    18301 gcttaggtgt gaaaacaggt atagatttac caaatgaaac aaaaggtcaa atcgaaccat
    18361 taacaaataa tccaggtaat tatctagatt tatcaattgg tcaatatgat acctatacac
    18421 cattacaatt atcacaatat gtttcaacta tagcgaatga tggttataga atacaaccac
    18481 acattggatt aacgattcat gaatcaacta ataaagatga tgttggtcca ctcaagaaga
    18541 aaattaatgg cactgtcttg aacaaggtta ataatactga aaaagaaatc aaacaaattc
    18601 aagaaggatt caaaatggca tttaatgata aagatggtac tggatatgtt agttttaaag
    18661 atacagtagt gcctactgct ggtaaaactg gtaccgctga agtgttccaa aacggagagc
    18721 caagagttaa ctctacttat ataggatacg cgccaattga tgatccaaaa ttagcgtttt
    18781 caattgtata tacaaatcag cctgtaccac caccatggtt aacaggtgga gacttaggta
    18841 gagatgtaat taactactac tttaagcagt taggtaaaga cgataaaaat aaagacaaag
    18901 acaaataaaa tttaacctga cgatt
//
LOCUS       DAMZPP010000044        18597 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMZPP010000044 DAMZPP010000000
VERSION     DAMZPP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18597
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.044"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(767..868)
                     /locus_tag="POH73_001163"
     CDS             complement(767..868)
                     /locus_tag="POH73_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887129.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNSVVIGGKGK"
     gene            953..2341
                     /locus_tag="POH73_001164"
     CDS             953..2341
                     /locus_tag="POH73_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDI6887130.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLVYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALMFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYIKKTLEVLIFAFTSRSSAGTLPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FESNDNAALTSH"
     gene            complement(2421..3176)
                     /locus_tag="POH73_001165"
     CDS             complement(2421..3176)
                     /locus_tag="POH73_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HDI6887131.1"
                     /translation="MSDHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEQIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVARVREILDLPDYVFPLFG
                     MAVGEPAEDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISKYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            3667..4236
                     /gene="ahpC"
                     /locus_tag="POH73_001166"
     CDS             3667..4236
                     /gene="ahpC"
                     /locus_tag="POH73_001166"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDI6887132.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            4252..5775
                     /gene="ahpF"
                     /locus_tag="POH73_001167"
     CDS             4252..5775
                     /gene="ahpF"
                     /locus_tag="POH73_001167"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDI6887133.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKELLTEIS
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGENHVTGIRYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            5890..7509
                     /locus_tag="POH73_001168"
     CDS             5890..7509
                     /locus_tag="POH73_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887134.1"
                     /translation="MLDVHFIYPGKLQNACEYFNLPTQKEGFEVTIHALRPENIHKIY
                     HDMDKTEQKFIETLISNGNPVTIINKRDVNILKLTNHLGFVYQINEDEFFVNNLFLSY
                     INSEVLDSTYGEFESLDTINHMNLNEEVLRVRQHTRLNDVTLEHALLLKTNDELKTIC
                     RQYGIKGFSNKNKQNLVELIMTAFFNNNHIIEQVFSNTTLFEFDILSSILESDANYRL
                     NNELIEFEKLQLINFDNFLTSHFYYYYDFEYDCLILPNDVRKHIENFVNQVGNGDFEK
                     AVNQFVDEQTIENSLAEFENALELSFMEDEVDLDMLLEHDDFEDFGTTDSLRSDAITQ
                     LISKIKKGLIDKKDILPLRIILGSVNLYGILSTNHLLYLTQRFYDATFSKEELQYSLD
                     TLAGTELYYIEDDFVFHPVLFDVVESEASGIDISDEGYYVPASVEELIYYDRHHYLKS
                     DQAIKEFTSYLRKQINMKNDIEKEKYVNEIIMLLRTIPTSKLVQPIMQLFIDNAILNN
                     VQNDALLIEKATHARNHLRLWSLQGHRDIPN"
     gene            7582..8208
                     /locus_tag="POH73_001169"
     CDS             7582..8208
                     /locus_tag="POH73_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HDI6887135.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVNEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            8479..8862
                     /locus_tag="POH73_001170"
     CDS             8479..8862
                     /locus_tag="POH73_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887136.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPALILAIFNNMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFISAIITLAIGGKSFDKNDSH"
     gene            complement(8928..9509)
                     /locus_tag="POH73_001171"
     CDS             complement(8928..9509)
                     /locus_tag="POH73_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDI6887137.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYEKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSREEAVDLKVENCKPYIIE"
     gene            9687..9896
                     /locus_tag="POH73_001172"
     CDS             9687..9896
                     /locus_tag="POH73_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887138.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILVFLFPTSSHVNLLLF
                     NPILPTFSNIIIHIDHFHANKKDDN"
     gene            complement(9935..10189)
                     /locus_tag="POH73_001173"
     CDS             complement(9935..10189)
                     /locus_tag="POH73_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDI6887139.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIIFIAIVTLILRAVRKK"
     gene            10483..10746
                     /locus_tag="POH73_001174"
     CDS             10483..10746
                     /locus_tag="POH73_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6887140.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            complement(10884..11456)
                     /locus_tag="POH73_001175"
     CDS             complement(10884..11456)
                     /locus_tag="POH73_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDI6887141.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVDD
                     KNKKVINKKTEKEDTVNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDQ"
     gene            complement(11637..12005)
                     /locus_tag="POH73_001176"
     CDS             complement(11637..12005)
                     /locus_tag="POH73_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDI6887142.1"
                     /translation="MKTILKTITYLAFTIIGAYAALFILKTIDSHGITDQFNPLVKED
                     DSYVKTTEVSTRMDDQLRSYSQSAFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            12292..12956
                     /locus_tag="POH73_001177"
                     /pseudo
     CDS             12292..12956
                     /locus_tag="POH73_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015977853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            12962..13969
                     /locus_tag="POH73_001178"
     CDS             12962..13969
                     /locus_tag="POH73_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDI6887143.1"
                     /translation="MNRKTPKTTDGLMRHIRDNKGIQINGSTEKNQLRNIGYFHGFKG
                     YNFFLNKEEELNFEKFSELHALYSFDTEIKNLFYKHVMFCETAIKNRLLEIVCVNSGF
                     DLDSLFQKSLTYYKSYSPGSSKYKKHLKNTTRLRRDIYDMIHEHCNSKPYIYHYIHQD
                     RTVPLYAVFELFTLGNLVFFTRCMSQNLNIEAQKQLKLYSPAFDQSKDILGDIIDCMK
                     GLRNAIAHNGVLYDCRFKTGETSNRLKEYLAVKMDIHNLDFDRIIDYLILLILICSGL
                     SYSKAELQRIIRQFEQNLDFLRSKISESTYDKIIGVRARPKLRSLKNFINNLDFYLKT
                     D"
     gene            complement(14928..15440)
                     /locus_tag="POH73_001179"
     CDS             complement(14928..15440)
                     /locus_tag="POH73_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887144.1"
                     /translation="MKQQMLSKVLLSTVVVMGSIAGSSLVMDDNAHAEQKSDNIGKLN
                     QKNESTLHLSFEKGIKGTVDKNGKLTLSDGKTSKVMPTNAKDKKGNDVVLVYKKVKDG
                     FDVQVIKSSQERKTNWVKCGLGTVGGAGTGGLGGASAASVIPGLGTVAGAIIGGVSGG
                     ATGAAASCFG"
     gene            complement(15558..15995)
                     /locus_tag="POH73_001180"
     CDS             complement(15558..15995)
                     /locus_tag="POH73_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HDI6887145.1"
                     /translation="MAGQSIFETGRRLKHVKENDLAHGEFGKWLEKVGLDKYQASRFI
                     KVANEQSKLHSSANLGLKALYQIATIPVEHREEKQQTSSGEMKTPYEMTNKEREEFKR
                     QLKQRDEENAQLQSQMEQAQRSEEIARKQYKYGLNNYIFTIKF"
     gene            complement(16137..17105)
                     /locus_tag="POH73_001181"
     CDS             complement(16137..17105)
                     /locus_tag="POH73_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDI6887146.1"
                     /translation="MKPFESHNKQLKILRRRGMEVPSSAKRDLENENYYNIINGYKDL
                     FLELDVNGNFLVPDKYKQGTHFKEVFSLYKLDRKFRNVLLEYLLVFETHIKSRISYYF
                     SEKYREPHSYLYFKNYSSDTSKTDSIVKMVATFSSVMSNRKNKPLKHYINTHNGVPLW
                     ILVNYLTLGNVSKMYSNLDDDLRLEVAKDYKRKLERDYKTRVQITPSDVDSILQQAHM
                     FRNVCAHEERLYDYKIDRAKSRANIFANYNKIYDKEYVPTMNGSYVFDLLISLCLFLN
                     KHDYIKLVKNMDKLISNYSHSFYTITIDDLYTKMNFPDQTKILDML"
     gene            complement(17382..17951)
                     /locus_tag="POH73_001182"
     CDS             complement(17382..17951)
                     /locus_tag="POH73_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDI6887147.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYSANSAHVTGCRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGDVPIFINIGEWDGD
                     DEELDKTVKDVSNANPNHTVIVDDIPLED"
     gene            complement(17948..18160)
                     /locus_tag="POH73_001183"
     CDS             complement(17948..18160)
                     /locus_tag="POH73_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HDI6887148.1"
                     /translation="MADDNIDDHIVKNHLEMIVDQVANDKEFYIFDSLIQGRSYKDIS
                     SVLECSEQSVRLWYETLLDKIVEVIE"
     gene            complement(18292..>18597)
                     /locus_tag="POH73_001184"
     CDS             complement(18292..>18597)
                     /locus_tag="POH73_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDI6887149.1"
                     /translation="IKIIETKARLQNYKNRSISNMVLLKTVLNHYTEKEQKQVVKYMR
                     SNGRYKPYNVIERLQVDLYQASIKQRSERQKQRNIAIENSKIARVNAYHQSSHVKVV"
BASE COUNT         6149 a         3297 c         2689 g         6462 t
ORIGIN      
        1 gactcagtaa tatatatcta tgatttaaaa actgatcttt tataaaacta tagaactgta
       61 atttattttc aaatttaata ctcaattcat aaaacctgaa acaatctgat aaactccttt
      121 caatatctac tttgaagaga gcatatcaaa ctaatttgtt tcaggttttt taattattca
      181 ttatgattaa tattgaattt tgaataaatt tttgtagtat gttactttta aatgggatgg
      241 cttctgtgta cttctaaata gattcatctt aataagccca ttcaatttaa atgtttggct
      301 gtgttagtaa atgacgaatc gcacaagggc acactcatat tcacatttac tacaaacgcc
      361 tttttgctca aacaaaaata tttgtttacg gtagcttttg aactaccacc tgcacacacg
      421 aatcctcaaa agatgctaat tttgattcga aagaattttt tacaacgtgt ttgttcgaag
      481 cgtcttttca aggttgatca tttattatct tttttattca ataaatatga ggttagcaat
      541 ttttatcaaa ccgccatagc cacatctcga ttcaaggata catttgtttt ctcatatctt
      601 atctctcatg acagtacacg atatatatct tttccaaata ttcatcactt ttttcctgtt
      661 aaaatcattt ctattattaa cgctgtaatg acaacttatt taatttattg catttaatgc
      721 cctgcttcat tttcaaatat taactttaat cgactagctc atcacactat ttaccctttc
      781 cacctatcac tactgaattc aaaacgattt gtttacaaat ccccttatgc caacggtcga
      841 atttcatcat gtttacattt aattgcaaat gccctcttga aataatttaa aattaatatt
      901 attcttagta tactggtcgg aattatattc aaaaatttga ggagtgacgt tcatgaatgc
      961 ttttcttaca ctgattaata ttattgtatt ggtgattttc atcgtaatat tgcacatgat
     1021 ggctcgtaaa catatatctt ttgcgaaacg tgtgtttaca gctttaggta tcggcattgt
     1081 atttggcgtg ttactacatc ttgtctatgg aacacattcg aatgttataa catcaacaag
     1141 tgactggttc aacattgtcg gacaaggtta cgttgcattg ttgcaaatga ttgtgatgcc
     1201 tttaattttc atttctattg tcgctgcatt cactaaaatt caaatcggag aaaaatttgc
     1261 taaaattggt agtctcattt ttatcttttt aatcggcact gtcaccattg ctgcaattgt
     1321 cggtgttgtg tatgccttga tgtttggttt agacgcatca acaattaatc ttggtaacgc
     1381 tgaacaagca cgtggaagcg aaattgctaa acaagcgaaa gatttaacag cacatacgtt
     1441 accacaacaa attttggaat tattgcctaa aaatccattc ttagatttca ctggacaacg
     1501 tgcaacttcc acgattgctg ttgtgatatt tgcttcgttt ataggatttg cttatttacg
     1561 cgtagcacgc aaacaacctg atcatggtga attgcttaag cgtgcgattg acgctatcta
     1621 ttcactagta atggcgattg tgacgttcgt cttacgttta acgccatacg gtgtattagc
     1681 aattatggcc aacacgttat cgacaagtga ctttggcgcc atttggactt taggaaaatt
     1741 cttaatcgca tcgtacgctg cattaattac gatgtatatc attcacttaa ttattttaag
     1801 cctgttaggc attagcccta ttcgctatat taaaaagact ttggaagtat taatctttgc
     1861 attcacatca cgctcaagcg cgggtacctt accactgaat gtacaaacac aaacaagacg
     1921 tctaggcgtg ccggagggta ttgccaattt cgctgcgaca tttggattgt cgattggaca
     1981 aaatggttgt gctggcattt atcctgccat gcttgcaatt atggtcgcac ccgtcgctaa
     2041 tgtagaaatt gatttacaat ttattgtaac gttaatcgct gtcgtgatta ttagttcatt
     2101 cggcgttgct ggcgtcggtg gcggggctac atttgcatcc atattggtgt tgtcaacgtt
     2161 aaacttgcca gtcgctttag ccggcgtgct catctccgtt gaaccattga ttgacatggg
     2221 tagaacagca ttaaacgtga acgattcaat gctagctggt acaggtaccg ccaagttaac
     2281 taaacattgg gacaaagata ctttcgaatc aaatgataat gctgcattaa cttcacacta
     2341 attcattgca agaaataccc gccccttcta gctatactta tcattttaag ctagaggggg
     2401 cgggttattt tggtatcttg ctatcgctgt attaagcctg atttttgcaa ttgttctaac
     2461 atatctaatc ttgctttgtt tcctaggaac atctcaattt gctgcgacca tgtttctttg
     2521 cgattcccgt ttgtacgttg atcatagtac ttgctgattg tctgatcgta atctgccatt
     2581 tgtgcatact gtgtttcctt atcagcatga tacttattat gatggaagac atggtcaaat
     2641 ggtaagcgtg gtttggctgc gccattttca tcttctgcag gttcccctac tgccatacca
     2701 aataatggaa agacatagtc cggtaagtct aaaatttctc gaactcgtgc aacatcattt
     2761 ctcaatgatc ctaaaaagac aatgccatac cccatatctt cagcagttac cgcaatattt
     2821 tcggcaacta atgctgcatc gattgcacct actagcaaac cttccgttga accatatgca
     2881 ttttccatat cagtttcagc atgttgatca actaaatgat gacgataata atcaataaca
     2941 aagacgaata aatagccatt ttctacaaca taaggttgtc cagaaacttc tcgtaaattt
     3001 tctttaatct gctcatcgtc gataccaata attgagtatg cttgtaggaa acttgacgtc
     3061 gaagcgcttt gtccagcttc taccaatttc ttaacaacgt cttcacttaa aggtttattc
     3121 ttaaattttc taacagaatg atgctttttc acaagattat atacatgatc tgacacgtta
     3181 ccactcctcg atactttaca gttaattaaa aatcttcata ttgcaattgt acacgtccaa
     3241 acttttttat agcaattatt tgcttataag ttaataattg aactttcaaa aatattgtct
     3301 cgtacttaaa aaatccagtt tctcacgcat taccttcatc catctcgaac aaatattctc
     3361 acaccattat caataaggtt gacttagttg agaatacaaa tcttcttttt ttacattttt
     3421 tacattaaaa tcaagatatt aaatatagac acaacctttt aaatcatcga ctacaaaatc
     3481 cattatcaaa gcactttata ttatctttct ttattttaaa agtaatcatt cactttacaa
     3541 agttatccag ttgactattc tggatcaaaa tgtaataatg ctaactaaga gattagaatt
     3601 attataatta agcacaatca attgatagtt ctaattcata cttaaattct taggaggaag
     3661 atatttatgt cattaattaa caaagaaatc ttaccattta cagcgcaagc tttcgatcca
     3721 aaaaaagatc aatttaaaga agttacacaa gaagatttaa aaggttcttg gagcgtagta
     3781 tgcttctatc ctgctgactt ctcattcgtt tgtccaactg aattagaaga cttacaaaac
     3841 caatatgaag aattacaaaa attaggcgta aatgtattct cagtatcaac tgatactcac
     3901 ttcgtacaca aagcatggca tgaccattca gatgcaatta gcaaaatcac ttacactatg
     3961 attggtgacc catcacaaac aatcactcgt aattttgatg tattagatga agctactggt
     4021 ttagctcaac gtggtacatt cattatcgac ccagacggtg ttgtacaagc atctgaaatt
     4081 aacgctgacg gaattggccg tgacgctagt acattagctc acaaaatcaa agcagctcaa
     4141 tatgttcgca aaaaccctgg cgaagtatgc ccagctaaat gggaagaagg cgctaaaaca
     4201 ttgcaacctg gtttagattt agtaggtaaa atctaaggag gcattacaac catgcttaat
     4261 gctgatttaa aacaacaact taaacaacta ttagaactaa tggagggcaa cgttgaattc
     4321 gttgccagcc ttggttcaga tgataaatct aaggaactca aagaattatt aacagaaatt
     4381 tctgatatgt cacctagact ttcactttct gaaaagtctt taaaacgtac accaagtttc
     4441 tcagtcaatc gtcctggcga agaaacaggt gtaacatttg caggtattcc attaggtcac
     4501 gagtttaact cacttgtttt agcaatttta caggttagtg gtcgtgcacc taaagaaaaa
     4561 cagtcaatca ttgaccaaat taaaaactta gaaggttcat tccattttga aacattcatt
     4621 agtttaacgt gtcaaaaatg tcctgatgtc gttcaagcac ttaacttaat gagtgtgatt
     4681 aaccctaaca tcacgcattc tatgattgat ggtgcagtgt tccgtgaaga atctgaaaac
     4741 atcatggcag tccctgctgt ctttttaaat ggcgaagaat ttggcaatgg tcgtatgaca
     4801 atccaagata ttctttcgaa actaggcagt acggcagatg catctgagtt tgaaaacaaa
     4861 gaaccttatg atgtcttaat cgttggtggt ggtcctgcta gtggtagtgc agcgatttac
     4921 acagcacgta aaggtttacg tactggtata gttgctgatc gtatcggggg ccaagttaat
     4981 gatactgctg gtattgagaa cttcattact gttaaagaaa caactggttc tgaattttct
     5041 tctaacttag cagcgcacat tgatcaatat gacattgatg caatgacagg cattcgtgct
     5101 acagatatcg aaaagactga cgaagcaatt aaagttacgt tagaaaacgg tgctgtccta
     5161 gaaagtaaaa cagtcattat tgctactggt gcaggttggc gtaagctaaa cattccaggt
     5221 gaagagcaat tgattaataa aggtgttgca ttctgccctc actgtgacgg acctctattt
     5281 gaaaataaag acgtagcagt tatcggtggc ggtaactctg gggttgaagc agcaattgac
     5341 cttgctggta tcgttaatca tgttacatta ttcgaattcg ctagcgaatt aaaagcagac
     5401 aacgtgttac aagatcgttt acgttcttta tcaaatgttg atatcaaaac aaatgccaaa
     5461 actactgaag ttgtcggaga aaaccatgtt acaggtatac gttacgaaga catgagcacc
     5521 ggcgaagaac atctacttaa cttagatggt atctttgttc aaattggttt acttccaaac
     5581 acatcatggt taaaagatgc tgttgaatta aacgaacgtg gtgaaattgt tattgatcgt
     5641 aacaataata ctaacgtacc tggaatattt gctgctggcg atgtcacaga tcagaagaac
     5701 aaacaaatta ttatttcaat gggcgctggt gcaaatgcag cattaaatgc ctttgactat
     5761 attatcagaa actaaagttt cattcatttc actgacagta tcttacattt gttaagatgc
     5821 tgttattttt ttatcccgac ataaaatcat gttatgataa acttaatata tacaacgagg
     5881 tgtgcaaaaa tgcttgatgt tcattttata tacccaggaa aattacaaaa tgcatgcgaa
     5941 tattttaatt taccgaccca aaaagaagga tttgaagtca cgattcacgc tttacgccct
     6001 gaaaatatac ataaaattta tcatgatatg gataagacag aacaaaaatt tatagaaaca
     6061 cttatatcta atggtaatcc tgtcacaatt ataaataaac gcgatgttaa tattttaaag
     6121 ctaacaaatc atctaggttt tgtttatcaa attaatgaag atgagttctt tgtaaataat
     6181 ttatttttaa gttatatcaa ttcagaagta ctagatagta cctatggtga attcgaatcg
     6241 ttagatacaa taaaccatat gaatttaaat gaagaagtgt tacgtgtaag acaacatacg
     6301 cgcttaaacg atgtcacatt agaacatgct ttactgttaa aaactaatga cgagctaaaa
     6361 acaatatgca ggcagtatgg tatcaaagga ttctcaaata aaaacaaaca aaatcttgtt
     6421 gaattaatta tgacagcatt tttcaataat aatcacatca ttgaacaagt gttttctaat
     6481 actactctat ttgagtttga cattctttct agcatacttg aaagtgatgc taactaccga
     6541 ttaaacaatg aactaattga atttgaaaag ctacaattaa taaattttga caacttttta
     6601 actagtcatt tttattatta ttacgacttt gaatatgatt gtcttatttt gccaaatgat
     6661 gtgcgtaaac atattgaaaa tttcgtgaat caagtcggca atggtgattt tgagaaagct
     6721 gtaaatcaat ttgttgatga gcaaacaatt gagaatagtt tagcagagtt tgaaaacgcg
     6781 ttagaattat catttatgga agacgaagtt gacttagata tgttacttga acatgacgat
     6841 tttgaagatt ttggtacaac cgatagctta agaagtgatg caatcactca attgatttct
     6901 aagattaaga aaggattaat agataaaaaa gatattttac ctcttcgtat tatacttggt
     6961 tctgtaaact tgtatggtat tttatcaact aatcatctac tatatctcac acaaagattt
     7021 tatgacgcta cttttagtaa agaagagttg caatatagct tagatacttt agctggtact
     7081 gagctatatt atattgaaga tgacttcgtg tttcaccctg tgttatttga tgttgtcgag
     7141 tctgaggcgt caggcattga tatttctgat gaaggatact atgtaccagc ttctgttgag
     7201 gaattaatct attatgatag acaccactat ttgaaatctg atcaagcaat taaagagttt
     7261 accagttatc tacgcaaaca aatcaatatg aaaaatgata ttgagaagga aaagtatgtg
     7321 aatgaaatca ttatgctttt acgtacaata cctacgtcta aattagtaca accaattatg
     7381 cagttattta tagataatgc tatccttaac aacgttcaaa acgacgcatt attgatagaa
     7441 aaagcaactc atgcacgtaa tcatttacgt ctatggtcat tgcaaggtca tcgtgacatt
     7501 ccaaactaat tcaatgttac tctattaaac atgaatacat attttttcaa ttaaagataa
     7561 actaaggagt tatattacat tatgaaaaag agattactac taagtacatt tttagcatcg
     7621 acattaattc ttacaggttg cgcgtccgat caatctgata acgaagatca tcacacaagt
     7681 actggcatcc atgcacctaa aagcgctaaa aaattagaaa caaaagatat ttttaactca
     7741 gataagaaaa atagtgatat tagtgatgca gagatgaagc aagctataga aaagtatttg
     7801 tctgtaaata gcgatatact tgataacaaa tatattatgc aacataaact agataaacaa
     7861 attgacagtc aaacaaaagt gactgaaaaa caagcagaaa cacttagcca cttatctaac
     7921 ttagctgtta aaaatgattt gcatttcaag aaatttgtca ctgaaaacaa tattcctaaa
     7981 gaatataaga aacccgttga attaatgatg aattacttca aagctttaaa tagtacaatc
     8041 gccaatgtta acgaagatat tgaaaagctt agctatcaac cgcaaaataa aatcaatgta
     8101 gtcgacgtgc caacaaaata tgccggtgat gttaataaaa agcaacaaga taaaattaaa
     8161 gatttcttaa aatctaaagg tatcaagagt gatgttatcg ataaataact gaaagccatc
     8221 attgcgacat gtaacacatc actcatcagt acatgcgctt taattcatga caattcttta
     8281 tctttaaaag tgactaatct ttgagacaat acattttaac caataccata ttgcatcaag
     8341 cagcatatta ctgtttgatg cttttttaat tgccttaaat tctctatcca tcctaaaatc
     8401 cccattagtt gacgcatttg actatatccc taacatattc catacttata ttgactctat
     8461 aaacaaagga tggtcatgat gaaagttcga atcactttta ttattttagc aattttatcg
     8521 acgattgttt gcttgttctt agcagcaatg catccaacag gtccaaacac agtaactttc
     8581 gaacagccat atttattcac actcaacatc attataatgg tattagtcgc attacccgca
     8641 cttattttag ctatattcaa taatatgagt tttagaattt cttctgctat tttacaattt
     8701 ctaggggcta tctcttggtt ctttttatca ttgatattat cgctcacaca atttacacct
     8761 tttacattag cgtcatttat aacttcaatt attttgttca taagcgcaat tatcacatta
     8821 gctattggtg ggaagtcttt tgataagaat gattcccatt aaaatccaaa tacaaaaaag
     8881 gttctgaagg ccgctataaa acacagcttt tcagaacctc tatacttcta ttcaatgata
     8941 tatggtttgc aattttctac ctttaaatcc acagcttctt cccttgaaac tttgttaaaa
     9001 taaaccatca aacaacgaat gacaacttga tgtgcaacaa tgacgatatc atctttttgt
     9061 gtatcttcat tgacaacatg attcataaaa tgttctacgc gttgatatac atcttcataa
     9121 ctttctcctt caggcgcttt ttgtgaaaaa ctatgtcgaa agtctttaaa gtttggatca
     9181 ttgaaatatt tttcatattt cggattcgca ctgatttcat ctttatattc accctcaaat
     9241 acgccaagtg aacgttctct taatagaggg gtagtcgttg atgcaatgtc atatggaaaa
     9301 atatgttcaa acgtttgctg tgttcttaat aagtctgaaa catatacatg tttaatctgt
     9361 ttctctttaa aataatcaca taaatcgtcc gcactttttg tgccagtatc cgttaacggc
     9421 acatctaatt gtccacaaaa ataagatcta aaatgtttat tatcataatt cgattttgat
     9481 tcgccatgtc taactaaata aatcgtcata atattactcc ttatcttatg tatttcatat
     9541 ctaccataac actttgacta ctaattcgat atcaatctta atattctatt ctaaaaaaag
     9601 aattaattca tattttaaat taatgactct aaaaatagta ttgtctttaa tcaagtgatg
     9661 tgttactata tgggtgtagt tcttaaatga ctacacagtc ttttatattc atgaacataa
     9721 tactcctgga tggcgcaacc caacgtcatc ctttttttat ttgtcaaata ctcgtttttt
     9781 tatttccaac ctcttctcat gttaatttat tactttttaa tcctatctta cctacatttt
     9841 caaatattat aatacatatt gaccattttc acgcaaataa aaaagatgac aactgaatgc
     9901 catctttata ttaattcaac ctaagttgaa tattttattt tttacgaacc gctcttaaaa
     9961 ttaacgttac gattgcaata aagataattg aaccgattaa agctggcaag atataaatac
    10021 ttcctaattc aggaccccat tgtccgaata gtttgccacc tacccatgat ccaataatac
    10081 ctgcgataat attacctaaa ataccacctg ggatatcttt acccataata gcaccagcag
    10141 cccatccaat taagccaccg acaattaaca ttccaataaa tccaaacata attttcagtc
    10201 tcctttttct atttattttg cgttattcta agtagtaccc attattagac tttctaaaac
    10261 aaattcaaat tatttttatc caaatatttt taaaagtagt aattgaatat caattttatt
    10321 caatgtagct atcgttattt aaagtctctg taccggcaat atcatataca ttattttttc
    10381 tgccgaattc atagcttgat tattttatgt tataggacta gaatatacac atattataag
    10441 agcatctttg aattttaaat caagaagcga ggttaatgaa caatgaatat gcatatttta
    10501 tataacttac gaactaaaca taatttagaa attgacgaat tagcacaaca attaaatgag
    10561 aaatatggta ctaaatatga agcacatcaa atttgggaat gggagaatca tcaccatgaa
    10621 cctaaattta aagatgccat gcatttagct gacttctttg atgcaccata tgaaatgttt
    10681 ttagaaagta aggttaaaga atatcagaaa catttagaag aagtcgatat tcgcatggat
    10741 aaatagatgc aattaaaccc tcacaacacg ttaggcatat atcctttcaa atctatacgg
    10801 gatatattac attgcgttgt gagggtattt taataattaa tatgaattaa gacatgttgc
    10861 catcgttagt gttacggcat attttattga tcttgctcac tctttaatac tttaccgttc
    10921 ttagcatcaa cagtaacttc ttgtttttta ttaccttttt tcaaatcgat attgtaaaca
    10981 agtttaccat catctttttc aagtgaccat tctttaatat caccatcaaa ttccttttgt
    11041 ccttctttaa tggctttttt gtaatctata gcatcgctat atttaaagtt atcattttca
    11101 ttcactgtat cttctttttc agtctttttg ttaatcactt ttttattttt atcatcaaca
    11161 agtacttctg actcttcacc agatttttgt tgtgtcactt tataagccca ttcaccatta
    11221 gaattttcaa atgaaattcc tttcaacttt tggcctttgt aagtttcttc agcttttttc
    11281 acagcatctt ctgggcttgt tttaacatct tttaatgcaa taacatcttt tgttgtatta
    11341 gtgtcttgat tagtatttga ctctgttgat tttgtttcat cttttggagt atcattgcca
    11401 catgcagtaa gcaccaccgc tgacattgat aacactgcta atgattttaa tttcataata
    11461 tcactctctc ttcctttttt tgaaactcat aacaaaagct tatatgctat atagattgta
    11521 ttaccccttg tttttaattc tattcataat tattacaaat atttttaaat taatcgtcat
    11581 gtgtcacttc atttcgtact catctttaaa atgaaccatg taatataaga atgctattat
    11641 cgactcagtt tgtctaatgc tttctttggt acttcttcct tttctacttc ttcaaaagtt
    11701 tctacatgat gacctttatg tgtaattttc aagtatctat gcggtttaac atcaaatgta
    11761 gcagtataca ttaattgcgt ctctttccct tctttattaa aagcactttg actataactt
    11821 cggagttgat catccattct agtagacacc tctgtcgttt taacataaga atcatcttcc
    11881 tttactaatg ggttaaattg atctgttata ccatgagagt ctattgtttt taaaatgaat
    11941 aaagcagcat aagcgccaat gatagtaaat gcaagatatg ttattgtttt taaaatcgtt
    12001 ttcaaaagat cacctctgac aaaatataaa ataggatgtc tctgaaaaca acttacataa
    12061 ttgtaattaa agtgtaagtc ttaggagaaa atgatggtat ttgaatctga aagatacgaa
    12121 tcgtttttaa actaagttat tttatagaga ctttattatg gaattgaatc gtctacactt
    12181 aattggacaa attctaagag aataaaattt gttaatttaa aatagtaagc aattcaaagt
    12241 tatatgtgta atagataaaa tagatatccc tatagtgatg cgttactaac tatatgtaat
    12301 aacaatttaa aagataatgt agtgaaagaa ataccggatt gtttcgacta tctaattcag
    12361 cataaaaata cccaacgaga aaacgtaatt ataaaaaggt aaaatctata gtgcagtttc
    12421 aaattgctaa tggcatgcgt atcggtgagc tacttgcaat aaaaagagaa aatataaact
    12481 atgaagataa aacgctagat atcgatggta caattaattg gataactgat taaaaatgga
    12541 agcatccgga gtaaaagaga caactagaac aagtaacagc tataggacta taggcctcac
    12601 tatccatagc atcgacttac taagaacact tatgctttga atgataagtt tattaatata
    12661 gggtacatat tcacaaatgc agctggtggt cctatcgatt taaacaaaat aaataacatt
    12721 attaaagggg gggctatcaa agagacaact gagattagtt ctattaagaa acctgcaacg
    12781 acgcatacat tacatcattc gcatatatct acacttgctc aattaggaat taacttaaaa
    12841 gcaatgcaag agcatgtagg tcattcagat tataaaaaaa tctagagata tacacacatg
    12901 ttactaatca gatggcgaaa gatatgctga ataaatttga acgattgggg agttaaaaac
    12961 tatgaacaga aaaacaccta aaactactga tggtttaatg agacatataa gagacaataa
    13021 aggtatccaa ataaatggta gcacggaaaa aaaccagtta cgaaacatcg gctattttca
    13081 tggattcaaa gggtataatt tctttttaaa caaagaagaa gaacttaatt ttgaaaaatt
    13141 cagcgaatta cacgctttat acagttttga tacagaaatt aaaaaccttt tctataaaca
    13201 cgttatgttc tgtgaaacgg ctattaaaaa tagattgcta gaaatagttt gtgtaaactc
    13261 tggctttgat ttagatagtt tatttcaaaa atcattaaca tattataaaa gttatagtcc
    13321 cggtagttca aaatataaaa aacatttaaa aaatactact agactaagaa gagatattta
    13381 cgacatgatc catgaacatt gtaattcaaa accttacatt tatcattaca tacatcaaga
    13441 tagaaccgtg ccactatatg ccgtatttga attattcact ttaggaaatc tggtattttt
    13501 cactcgttgc atgtcccaaa atttaaatat agaagctcaa aaacaactaa aattatactc
    13561 cccagcattt gatcaaagta aagatatact aggtgacatc attgattgta tgaaaggctt
    13621 aagaaatgct atagctcaca atggtgtact ttatgactgt agatttaaaa ctggagagac
    13681 aagtaataga ttaaaagaat atttagcagt aaaaatggat atacacaatt tagatttcga
    13741 cagaataatt gattatctca ttttattaat tctaatttgt tctggtctct cttattctaa
    13801 agcagaactc caaagaatta taaggcaatt cgaacaaaac cttgattttt tacgatcaaa
    13861 gataagcgag tcgacttatg ataaaattat aggcgttcga gcaagaccta aattaagaag
    13921 tttaaagaac tttatcaata accttgattt ttatctaaaa acagattaat attatacata
    13981 aatagcggtg catatctgcg gaatgcactt gaggttaact gttactgacg gttgcctctt
    14041 tttttatgca tctaaattca tattattttt gcaatataaa catatctttg tgcaaattcc
    14101 gaacacaaaa cattcacatc ctcttttttg cccttcatct tttatcccaa aacacaaaaa
    14161 caaccccata agcctatgct tacggggttt tacaataaac taaatattat tgttcttctt
    14221 ttatatactg ctattttatg aataatcatt agtgttcaaa aagctgttaa atcaatgttt
    14281 catactcata ttgttcattt tgaaagtcta tgaacattca cactatttcg taactttgcg
    14341 aactttttgc gaacatacta cccttgcccc tagtttcaca attacacctt ttttcgcctt
    14401 tataacaacc acactcctag atcaataggt tgtaaggttt gaatgtacct atagcactga
    14461 acattttaat gggctatact tctcaacaat tcacatcact tcataattaa cgtattgcaa
    14521 tatgtattca ccgcctgcaa agtatgaggt catattttta tatcaatgta caaaaaactg
    14581 ttttgccctt aaaacaacca cattcctgat taataggtag tttagtttga gcattttata
    14641 attaacataa aaaatagaca agtaccgaag tacctgcctt atttctaaat ccacaggaat
    14701 tcattaatat aataattata attgtaactt aattaatgtc tattatagtt atatataata
    14761 gaagcaaaac ctaaaatgac tataaaagct atcaaaacat aaactgaaaa attatcagtt
    14821 ctaatataaa acttaaaaag taagaacatt acaaaagcaa ctataaaata gacaggaata
    14881 aacgactttc gccaaagtat agagtttttc atgaattcac tctcctatca accgaaacat
    14941 gacgctgcgg cacctgtggc accaccagaa accccaccaa taatagcacc tgcaacagtt
    15001 cctaaacctg gtataactga agctgcacta gcaccgccta gcccaccagt gccagcgcct
    15061 ccaactgttc ctagaccaca tttaacccag ttagtttttc tctcttgact agatttaatt
    15121 acttgaacat caaatccatc tttaaccttt ttataaacca aaacaacatc gttacctttt
    15181 ttatctttag cattagttgg catcactttt gacgtttttc catcagataa tgttaactta
    15241 ccatttttgt caacagtccc tttaataccc ttttcaaatg aaagatgcaa ggtactttca
    15301 tttttttgat tcagtttccc gatattatca cttttttgtt cagcatgagc gttgtcatcc
    15361 attacaagag aagatcctgc tattgatccc ataactacga ctgtacttaa taatactttt
    15421 gataacattt gctgtttcat aataaaaact ccttttaaaa tttttacatt ttaattttat
    15481 cagttcttac tataacgctt taccaaaatt tcttaagtgc aataaaatca ttcctaattg
    15541 taaaaaatgg cgtgtgtcta aaattttata gtaaaaatat aattatttaa tccatattta
    15601 tattgctttc tcgctatctc ctccgaacgt tgtgcttgtt ccatttgtga ttgaagttgt
    15661 gcgttttctt catcgcgttg tttgagttgg cgcttaaatt cttcacgttc tttattggtc
    15721 atttcgtatg gtgttttcat ctctcctgaa gacgtttgtt gtttttcttc tcgatgctct
    15781 actggaatag ttgctatctg ataaagcgct ttaagtccta aatttgcgct cgagtgcaat
    15841 tttgattgtt catttgcaac tttgataaac ctgctagctt ggtacttatc taacccaact
    15901 ttttcaagcc acttaccaaa ctctccgtgc gctaagtcat tttctttcac atgcttcaat
    15961 cttcggccag tctcgaatat cgactgacca gcgatgtttg aagtcgtatt tcacgacgtc
    16021 acacttacga ttagcatatc tagttacaga atcttatccc ccactgcaac acagggcgtt
    16081 tctcagcgtc ttaaaataaa aaaacgccac tcgtaagtga cattaaaaaa tatcttttat
    16141 aacatatcca gtatttttgt ttgatctgga aaattcattt ttgtatatag gtcatctata
    16201 gtaattgtat agaaagaatg tgaataatta cttattagtt tatccatatt tttcaccaat
    16261 tttatgtaat catgtttatt caaaaataga catagtgaaa tcaacaaatc gaatacataa
    16321 ctaccattca ttgtaggaac gtattcttta tcgtatattt tgttataatt ggcgaatata
    16381 ttagctctac ttttagccct gtctatttta taatcataca atctttcttc atgcgcacac
    16441 acgttacgaa acatatgtgc ttgttgtagt atactgtcta catctgatgg agttatttga
    16501 acacgtgttt tataatctct ttccaatttc cttttatagt ctttagcaac ttccaatcga
    16561 agatcatcat ccaaattgga atacattttt gaaacattac ctaaagttaa ataattcacc
    16621 aatatccata gtggcactcc attatgagta ttaatataat gttttaatgg tttatttttt
    16681 ctattactca taaccgagct aaatgtagca accattttca cgatgctatc tgtcttactt
    16741 gtgtcagatg aataattttt aaagtataaa tatgaatgtg gttctctata tttttcgcta
    16801 aaataatatg aaattcttga tttaatatga gtttcaaata ctaacaaata ctctaataaa
    16861 acattcctaa attttctatc tagtttgtat aaagaaaaga cttctttaaa atgagtacct
    16921 tgcttatatt tatcaggaac caaaaaatta ccattaacat ctagttctaa aaataaatct
    16981 ttataaccat ttatgatatt ataataattt tcattttcta aatctctttt agcactactc
    17041 ggtacttcca ttcctcttct tcttagaatt ttcaattgtt tattatgact ttcaaatggc
    17101 ttcataaatt tacccctata aaagtagcca taacccgaat agagttatgg ctagatcgtt
    17161 atatatataa tacatttaat tttatcggtt gtaaataaat aagaaataac aaaaagagat
    17221 attttacaca attacctcct ttattttatt gctactcctc aaacccacca atattatcaa
    17281 taaacactgg tgttgttaca tttaagtcta ctttctcagt aaataagcta tggtatcaaa
    17341 ctaattagta ttaatttata aatagcatag cttcattttc ttcaatcctc taacggtata
    17401 tcatccacaa tcacagtatg gttaggatta gcgttagata catcttttac agttttatct
    17461 aattcctcat cgtctccgtc ccattcacca atgttaatga atataggaac atccccgttg
    17521 atatcatgct tatctgtaaa taacttatgg tatttaccca acatatcacg agcttttaaa
    17581 cgatcacttg gcttaattgg cacctctatc agttcaacat gttcattata gactaactgt
    17641 actttgccac tttgtggatt ctctttatat tccccacgct tgaccacaac ttctttcgtt
    17701 tctgtttcgt caccgactgc cgcattcgta agcacatgta gtaactcttt tgcagttaat
    17761 acattctcat ctataatctt atctttttgt tcttgtatat attgcttgat gtgtggcttc
    17821 tttaataacc tacaccctgt cacatgtgca ctatttgcgc tataacctgc ttttatggca
    17881 ctttgtgtca cattaagtgt tcttatatac tcattcacaa aacgcgcttg ctttgccgtt
    17941 aactcactca ttctatcacc tccacaattt tatctaataa ggtttcatac cataatctta
    18001 cagattgttc tgaacactct aagacactac taatatcttt ataactacgt ccttgtatta
    18061 aagaatcgaa aatataaaac tctttatcat tagctacttg gtcaacaatc atttctaagt
    18121 gattctttac aatatgatca tcaatgttat cgtctgccat ccattcatta gaattttcat
    18181 cacctattga aaagaattca tcagtattta tatcatcatc tattaataca tcacttctag
    18241 ttcgctcatg ataatcacaa acgaagcctt ttatttgctg tttatccatt gttacaccac
    18301 ttttacatgt gaagattggt gataagcatt tactcgtgca atcttgctat tttcaattgc
    18361 tatatttctt tgtttttgac gttctgaacg ttgtttaata cttgcttgat acaaatcaac
    18421 ctgtaagcgt tcaatgacat tgtagggctt atatcgtcca tttgaacgca tatattttac
    18481 aacttgcttc tgctcttttt ctgtataatg atttagtact gttttcaaca acaccatatt
    18541 acttatagat cgatttttat agttttgtaa tcttgccttt gtttcaataa ttttgat
//
LOCUS       DAMZPP010000045        18546 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMZPP010000045 DAMZPP010000000
VERSION     DAMZPP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18546
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.045"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..579)
                     /gene="rsmG"
                     /locus_tag="POH73_001185"
     CDS             complement(<1..579)
                     /gene="rsmG"
                     /locus_tag="POH73_001185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDI6887150.1"
                     /translation="MTVEWLAEQLKEHNIELTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNYLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGN"
     gene            complement(579..2456)
                     /gene="mnmG"
                     /locus_tag="POH73_001186"
     CDS             complement(579..2456)
                     /gene="mnmG"
                     /locus_tag="POH73_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDI6887151.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILEILEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(2523..3902)
                     /gene="mnmE"
                     /locus_tag="POH73_001187"
     CDS             complement(2523..3902)
                     /gene="mnmE"
                     /locus_tag="POH73_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDI6887152.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(4046..4399)
                     /gene="rnpA"
                     /locus_tag="POH73_001188"
     CDS             complement(4046..4399)
                     /gene="rnpA"
                     /locus_tag="POH73_001188"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDI6887153.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(4520..4657)
                     /gene="rpmH"
                     /locus_tag="POH73_001189"
     CDS             complement(4520..4657)
                     /gene="rpmH"
                     /locus_tag="POH73_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDI6887154.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            5388..6749
                     /gene="dnaA"
                     /locus_tag="POH73_001190"
     CDS             5388..6749
                     /gene="dnaA"
                     /locus_tag="POH73_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDI6887155.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKEATKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            7027..8160
                     /gene="dnaN"
                     /locus_tag="POH73_001191"
     CDS             7027..8160
                     /gene="dnaN"
                     /locus_tag="POH73_001191"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDI6887156.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            8541..8786
                     /gene="yaaA"
                     /locus_tag="POH73_001192"
     CDS             8541..8786
                     /gene="yaaA"
                     /locus_tag="POH73_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDI6887157.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            8783..9895
                     /gene="recF"
                     /locus_tag="POH73_001193"
     CDS             8783..9895
                     /gene="recF"
                     /locus_tag="POH73_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDI6887158.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            9905..11839
                     /gene="gyrB"
                     /locus_tag="POH73_001194"
     CDS             9905..11839
                     /gene="gyrB"
                     /locus_tag="POH73_001194"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDI6887159.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEDKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            11876..14536
                     /gene="gyrA"
                     /locus_tag="POH73_001195"
     CDS             11876..14536
                     /gene="gyrA"
                     /locus_tag="POH73_001195"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDI6887160.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDSSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESETDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEIISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDADEENEDEQSTVSEDGTEQQREAVVNDETPGNAIHTEVIDSEV
                     NDEDGRIEVRQDFMDRVEEDIQQSSDDDEE"
     gene            complement(14624..15436)
                     /locus_tag="POH73_001196"
     CDS             complement(14624..15436)
                     /locus_tag="POH73_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDI6887161.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            15762..17276
                     /gene="hutH"
                     /locus_tag="POH73_001197"
     CDS             15762..17276
                     /gene="hutH"
                     /locus_tag="POH73_001197"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HDI6887162.1"
                     /translation="MTLYLDGETLTIEYIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQMNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQAEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANATFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYDEFRSIVPSITYDRQFHKDIEAVAQYLKQSIYQTTACH"
     misc_binding    17349..17564
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17654..>18546
                     /gene="serS"
                     /locus_tag="POH73_001198"
     CDS             17654..>18546
                     /gene="serS"
                     /locus_tag="POH73_001198"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDI6887163.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDSKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNA"
BASE COUNT         6301 a         3052 c         3290 g         5903 t
ORIGIN      
        1 gttaccacct aacacactaa ttgcaaattt tgcttcttct aattcttctt caccttttga
       61 agattttaat gcaacaaact gtccaccttt tttaactagc ggtaaacaca attcactcaa
      121 cacggataat ctagctactg ctcttgcagt aacaacatca taagactccc tgtagacacc
      181 cttaccaaat gtttctgctc tatcgtgtat aaagctgaca tcctgtaatt gtaattctga
      241 cgctaaatag tttaaaaatt gaatacgctt atttaatgaa tcaacaatcg tcacttttaa
      301 ctgcggaaac attattttta acggaatact tggaaaacca gctccagcgc ctacatcaca
      361 tatacttata ggctgattaa aatcaaaata aaaactaggt gcaatggaat cataaaaatg
      421 tttcaaatat acatcgtgtt catctgtaat acttgtcaaa ttcatctttt cattccattc
      481 aacaagtaaa cgataatatg tttgaaactg ttgtttttga gtctcagtta attcaatatt
      541 atgttctttt aattgttctg ctaaccattc tacagtcatt agtcactcac cctttggagt
      601 ttaccttgtt ctaagtaaat caataatata gatatgtctg ctggatttac ccctgatatt
      661 ctagaagctt gtgcaatatt taaaggtttt acttctgata atttttctcg cgcttcagtc
      721 gccaaactat caatcttact ataatctaag tcttctggaa ttttcttctc ttccatacgt
      781 ttaactttct caacttgttg tagtgattta ttgatataac cttcatattt tgtttgtatt
      841 tctacttgtt cttcaacatc tgcattcaat tgatgttctt cttctaaaat ttctaaaatt
      901 atatcgtaag tcatttcagg tctgcgtaat aaatcgatag ctaaaatacc atcttttaag
      961 cgagaaccac catgttgttc aataatcgct tgcgtatgtt cgtttggttt aatacgaata
     1021 tctgataaac gcttaatttc cgcatcaatt tgctgacgtt tttcattaaa acgtgcatat
     1081 cgttcttcag aaatcatacc aagttcatat cccatatccg tcaatctcaa atcagcatta
     1141 tcatgacgta gtaacaaacg atattctgca cgtgatgtta gtaaacgata aggttcatta
     1201 gtacctttag ttacaagatc atcgattaat acacctatat atgcatctga acgacttaat
     1261 atcttttcgc ctgtgtttaa cactttacct gcagcgttaa tacctgccat caatccttgt
     1321 cctgctgctt cttcataacc agatgtacca ttaatttgac ctgcagtgta taggttttta
     1381 atcattttcg tttcaagtgt aggccataac tgcgttggca caatcgcatc atattcaatt
     1441 gcgtagccgg cacgcatcat atctgctttt tcaagacctg gtatcgtctc taacatttga
     1501 cgttgcacat gttcaggaag acttgtagac aatccttgca catatacttc atttgtatta
     1561 cgaccttcag gctctaagaa aagttgatgt cgcggcttat cattaaatcg aacaaattta
     1621 tcttcaattg aagggcaata acgtggcccg gttcctttaa tcatccctga atacattgca
     1681 gatagatgta aattatcatc gataactttg tgtgtttcag cattagtata cgttagccaa
     1741 catggcaatt gatctaatat atattctgtt gtttcaaagc tgaatgcacg acccacatcg
     1801 tcacctggtt gtatttcagt cttcgaatag tcaattgttt ttgaatttac acgcggtggt
     1861 gtacctgttt taaaacgaac aatatcaaaa ccaagttctc ttaaattgtc tgataatgtg
     1921 attgatggta attggtgatt tggtccactt gaatacttca tattacctaa aatgatttca
     1981 ccacgtaaaa atgttcccgt tgtaataatt actgctttag ataaatactc tgtaccaata
     2041 tttgtacgta cacctttaac ttcattatct tctataataa gttcgtctac cataccttgc
     2101 attatatgca aattttcttc atcttcaatc acgcgtttca tttcttgttg ataaagtact
     2161 ttatctgctt gcgctcttag tgctcttaca gcaggtcctt taccagtatt taacattctc
     2221 atttgaatgt gtgttttatc gattgttttt gccatttgtc cacctaaagc atcaatttca
     2281 cgaacaacga tacctttagc tggtccacct acagatgggt tacatggcat aaatgcaata
     2341 ttatctaaat ttattgttag cattaatgtt ttagcaccac gtcttgcaga tgctaaacct
     2401 gcttctacac ctgcatgtcc cgcacctata acgattacat catattcttg aaccacaata
     2461 taaacctcct tatttgatat cttactagcc ttcttaagac ggtattccgt ctatttcaat
     2521 tactatttac ctaagcagaa ttgactgaat aactgatcga tgagttcatc acttgcagtc
     2581 tcaccaataa tttctcctaa tatttcccaa gttctagtta aatcgatttg taccatatcc
     2641 ataggcacac cagattctgc tgcatcaatc gcatcttgta tcgtttgtct tgcttgtttt
     2701 aataatgaaa tatgtcttga attagaaaca taagtcatat cttgattttg tacttctcca
     2761 ccaaagaaca aatctcgaat ttgtatttct aattcatcaa taccttcttg ttttaacatt
     2821 gaagtttgaa ttaatggcgt atcgcctatc atatctttaa cttcattaat atctatattt
     2881 tgctctaaat ccattttatt aacaattacg attacatctt catttttaac cacttcatat
     2941 aatgtgtaat cttcttgagt caatgcttcg ttattgttta atacaaataa aattaagtcc
     3001 gcttggctaa gagcctttct agagcgttca acaccaatct tctctactat atcttctgtc
     3061 tcacgtatac cagcagtatc aactaatctt aatggcacgc cacgaacatt gacgtactct
     3121 tctaagacat ctctagtcgt acctgctacc tcagttacaa tcgctttatt atcttgtatt
     3181 aaattattta acatcgatga tttacctaca tttggtttac caacaataac tgtagataaa
     3241 ccttcacgca taattttacc ctgcgcaccg gtatctaata aacgattaat ttcctgtttg
     3301 atttctttag actgctctaa aagaaattca gtagtcgcat cttcaacatc atcgtattca
     3361 ggataatcaa tattcacttc cacttgagcg agtatctcta atatagattg acgttgtttt
     3421 ttgattaagt cacttagacg accttcaatt tgattcatcg caactttaga agctctatct
     3481 gttttcgagc gaataaagtc cataactgcc tcagcttgag ataaatcaat acgaccattt
     3541 aaaaaggcac gttttgtaaa ttcacctggt tcagccattc tagcgccata tgtcatagta
     3601 agttccagca ctctattaat cgttaaaata ccaccatgac aattaatttc tataatatct
     3661 tcgcgtgtaa atgtttttgg tgctcttaac acagacacca taacttcttc aaccacttct
     3721 ttagactctg gatcaataat atgaccgtaa ttaatcgtat gtgatggaac atcatttaaa
     3781 agatgttttc ctttatataa tttgtcagca atttcaacgg cttgcggtcc agacaatcga
     3841 acaattccaa ttgccccttc acccattggt gttgaaatac tcgtaattgt atctaaatcc
     3901 atattgctac tcgcctcctt caacgatgtg aatacatttt aaagtaaatt attataactc
     3961 taaggtaagt cttacgtttg tctgaggtaa gactacgggg tgtgttgagt ggttaatgtt
     4021 ttcctctctt tcccctaccc tatccttact taatcttttt attaaaaact ttggcaattt
     4081 taagtacgtg ctcaagacta ttctgtattt gtaaagtggt catatcttta gctggctgtc
     4141 ttgctattac aataatatct ttggccaata tatgcgactt atgtactttg aaattttcac
     4201 gtattgctct tttaatcttg tttcttaaca ctgcattacc tagtttttta gaaacactaa
     4261 tacctaagcg aaaatggtct atttctttat tattacaagt gtatacaaca aattgtctgt
     4321 tggctacaga atgacctttt ttatatattc tctgaaaatc tgcattcttt ttaattcggt
     4381 aagctttttc caataacatc actcgcttat ttatcgtttt tatttgaagc tatatttaaa
     4441 cttctattga gcttataaca taaatttcta tttattctta atttaaacga aaaaaaagat
     4501 cactgatgag tcagtgatct tatgcagata aaactttacg gcctttacga cgacggcgcg
     4561 ctaaaacttt acggccattt tttgtgctca tgcgttttct gaaaccatga actttactat
     4621 gtttacgttt atttggttga taagtacgtt ttaccatgca aaacacctcc atctttatac
     4681 agttatctaa tataccagat tatgcagact ctgatcactt taatagataa gattttgcgt
     4741 ttcttagatt aaaattctcg tattagctca ttgattatct agtcataatt caagcaacta
     4801 ctacaatata acaaaatcct ttttataacg caagttcatt ttatactact gctcaatttt
     4861 tttactttta tcgattaaag atagaaatac acgatgcgag caatcaaatt tcataacatc
     4921 accatgagtt tgatccaaag catgagtgtt tacaatgttt gaatacctta tacagttctt
     4981 atacatactt tataaattat ttcccaagct gttttgatac acacactaac agatactcta
     5041 tagaaggaaa agttatccac ttatgcacac ttatactttt tagaattgtg gataattaga
     5101 aattacacac aaagttatac tatttttagc aacatattca caggtatttg acatatagag
     5161 aactgaaaaa gtataattgt gtggataagt cgtccaactc atgattttat aaggatttat
     5221 ttattgatat ttacataaaa atactgtgca taactaataa gcaggataaa gttatccacc
     5281 gattgttatt aacttgtgga taattattaa catggtgtgt ttagaagtta tccacggttg
     5341 ttatttttgt gtataactta aaaatttaag aaagatggag taaatttatg tcggaaaaag
     5401 aaatttggga aaaagtgctt gaaattgctc aagaaaaatt atcagctgta agttactcaa
     5461 ctttcctaaa agatactgag ctttacacga tcaaagatgg tgaagctatc gtattatcga
     5521 gtattccttt taatgcaaat tggttaaatc aacaatatgc tgaaattatc caagcaatct
     5581 tatttgatgt tgtaggctat gaagtaaaac ctcactttat tactactgaa gaattagcaa
     5641 attatagtaa taatgaaact gctactccaa aagaagcaac aaaaccttct actgaaacaa
     5701 ctgaggataa tcatgtgctt ggtagagagc aattcaatgc ccataacaca tttgacactt
     5761 ttgtaatcgg acctggtaac cgcttcccac atgcagcgag tttagctgtg gccgaagcac
     5821 cagccaaagc gtacaatcca ttatttatct atggaggtgt tggtttagga aaaacccatt
     5881 taatgcatgc cattggtcat catgttttag ataataatcc agatgccaaa gtgatttaca
     5941 catcaagtga aaaattcaca aatgaattta ttaaatcaat tcgtgataac gaaggtgaag
     6001 ctttcagaga aagatatcgt aatatcgacg tcttattaat cgatgatatt cagttcatac
     6061 aaaataaagt acaaacacaa gaagaatttt tctatacttt taatgaattg catcagaata
     6121 acaagcaaat agttatttcg agtgatcgac cgccaaagga aattgcacaa ttagaagatc
     6181 gattacgttc gcgctttgaa tgggggctaa ttgttgatat tacgccacca gattatgaaa
     6241 ctcgaatggc aattttgcag aagaaaattg aagaagaaaa attagatatt ccaccagaag
     6301 ctttaaatta tatagcaaat caaattcaat ctaatattcg tgaattagaa ggtgcattaa
     6361 cacgtttact tgcatattca caattattag gaaaaccaat tacaactgaa ttaactgctg
     6421 aagctttaaa agatatcatt caagcaccaa aatctaaaaa gattaccatc caagatattc
     6481 aaaaaattgt aggccagtac tataatgtta gaattgaaga tttcagtgca aaaaaacgta
     6541 caaagtcaat tgcatatcca cgtcaaatag ctatgtactt gtctagagag cttacagatt
     6601 tctcattacc taaaattggt gaagaatttg gtgggcgtga tcatacgacc gtcattcatg
     6661 ctcatgaaaa aatatctaaa gatttaaaag aagatcctat ttttaaacaa gaagtagaga
     6721 atcttgaaaa agaaataaga aatgtataag taggaaactt tgggaaatgt aatctgttat
     6781 ataacagcac taataataac aatcattttt tacatttcta tatgctaatg tggcaagatg
     6841 agcaaaactc attttgtgga taatgtttaa aattcataca cgccatacac aagttatcaa
     6901 catgtgtata acttcgccaa atctatgttt ttaagactta tccaccaatc cacagcacct
     6961 actactatta ctaagaactt aaaacttata taattatata taaacgactg gaaggagttt
     7021 taattaatga tggaattcac tattaaaaga gattatttta ttacacaatt aaatgacaca
     7081 ttaaaagcta tttcaccaag aacaacatta cctatattaa ctggtatcaa aatcgatgca
     7141 aaagaacatg aagttatatt aactggttca gactctgaaa tttcaataga aatcactatt
     7201 cctaaaactg tagatggcga agatattgtc aatatttcag aaacaggctc agtagtactt
     7261 cctggacgat tctttgttga tattataaaa aaattacctg gtaaagatgt taaattatct
     7321 acaaatgaac aattccagac attaattaca tcaggtcatt ctgaatttaa tttaagtggc
     7381 ttagatccag atcaatatcc tttattacct caagtttcta gagatgacgc aattcaattg
     7441 tcggtaaaag tgcttaaaaa cgtaattgca caaacgaatt ttgcagtgtc cacctcagaa
     7501 acacgcccag tactaactgg tgtgaactgg cttatacaag aaaatgaatt aatatgcaca
     7561 gcgactgact cacaccgctt ggctgtaaga aagttgcagt tagaagatgt ttctgaaaac
     7621 aaaaatgtca tcattccagg taaggcttta gctgaattaa ataaaattat gtctgacaat
     7681 gaagaagaca ttgatatctt ctttgcttca aaccaagttt tatttaaagt tggaaatgtg
     7741 aactttattt ctcgattatt agaaggacat tatcctgata caacacgttt attccctgaa
     7801 aactatgaaa ttaaattaag tatagacaat ggggagtttt atcatgcgat tgatcgtgca
     7861 tctttattag cacgtgaagg tggtaataac gttattaaat taagtacagg tgatgacgtt
     7921 gttgaattgt cttctacatc accagaaatt ggtactgtaa aagaagaagt tgatgcaaac
     7981 gatgttgaag gtggtagcct gaaaatttca ttcaactcta aatatatgat ggatgcttta
     8041 aaagcaatcg ataatgatga ggttgaagtt gaattcttcg gtacaatgaa accatttatt
     8101 ctaaaaccaa aaggtgacga ctcggtaacg caattaattt taccaatcag aacttactaa
     8161 aaataaatat aaataaagga tgacgtgatt aattaaaacg tcatccttta ttttttggca
     8221 aaaataattc tagattcgta tgtaaaataa atttgacagc attttaaaca gcaaataaaa
     8281 gacgccaatt aaatttatga caaatgtatc cagaatttaa taagtgtgct tatatgccct
     8341 ttaaattcaa aattttaata gtcaataaca agttgaatat aaaagttaaa cgccgttaaa
     8401 tagcgttaaa aaattgaaaa tgacagtatt gccaaaaaat aagaattaat tatttatatg
     8461 taaacggttt ccacctctat tttaaatgaa atttgtgaca aaaaaaggta taatatatta
     8521 atgacataca aagaaatgga gtgattattt tggttcaaga agttgtagta gaaggagaca
     8581 ttaatttagg tcaatttcta aaaacagaag ggattattga atctggtggt caagcaaaat
     8641 ggttcttgca agacgttgaa gtattaatta atggagtgcg tgaaacacgt cgcggtaaaa
     8701 agttagaaca tcaagatcgt atagatatcc cagaattacc tgaagatgct ggttctttct
     8761 taatcattca tcaaggtgaa caatgaagtt aaatacactc caattagaaa attatcgtaa
     8821 ctatgatgag gttacgttga aatgtcatcc tgacgtgaat atcctcattg gagaaaatgc
     8881 acaaggaaag acaaatttac ttgaatcaat ttatacctta gctttagcaa aaagtcatag
     8941 aacgagtaat gataaggaac tcatacgttt taatgctgat tatgctaaaa tagaaggtga
     9001 gcttagttat agacacggca cgatgccatt aacaatgttt ataactaaaa aaggtaaaca
     9061 agtcaaagtg aatcacttag agcaaagtcg tctaactcaa tatattggac acctcaatgt
     9121 ggttctattt gcgccagaag atttgaatat tgtaaaaggc tctcctcaaa taagacgacg
     9181 ctttatagat atggagttgg gccaaatttc tgctgtttac ttaaatgatt tagctcaata
     9241 ccaacgtatt ttaaagcaaa agaataatta cttaaagcag ttacaattag gccaaaaaaa
     9301 ggacttaaca atgttggaag tattaaatca acagtttgct gaatatgcaa tgaaagtaac
     9361 tgataaacgt gcacatttta ttcaagagct agagtcgtta gctaaaccga ttcatgctgg
     9421 tatcacaaat gataaagaag cgttgtcgct gaattattta cctagtctta aatttgatta
     9481 tgctcaaaat gaagcggcac gacttgaaga aattatgtct attcttagcg ataatatgca
     9541 aagagaaaaa gaacgaggca ttagcttatt cggaccacat cgagatgata taagttttga
     9601 tgtgaatggc atggatgctc aaacatatgg ttctcaagga cagcaacgta caacggcttt
     9661 gtccattaaa ttagctgaaa ttgagttaat gaatatcgaa gttggggaat atcccatctt
     9721 attattagac gatgtactca gtgaattaga tgattcgcgt caaacgcatt tattaagtac
     9781 gattcagcat aaagtacaaa catttgtcac tacgacatct gtagatggta ttgatcatga
     9841 aatcatgaat aacgctaaat tgtatcgtat taatcaaggt gaaattataa agtaacagaa
     9901 agcgatggtg actgcattgt cagatgtaaa caacacggat aattatggtg ctgggcaaat
     9961 acaagtatta gaaggtttag aagcagtacg taaaagacca ggtatgtata taggatcgac
    10021 ttcagagaga ggtttgcacc atttagtgtg ggaaattgtc gataatagta tcgatgaagc
    10081 attagctggt tatgcaaatc aaattgaagt tgttattgaa aaagataact ggattaaagt
    10141 aacggataac ggacgtggta tcccagttga tattcaagaa aaaatgggac gtccagctgt
    10201 cgaagttatt ttaactgttt tacatgctgg tggtaaattc ggcggtggcg gatacaaagt
    10261 atctggtggt ttacatggtg ttggttcatc agttgtaaac gcattgtcac aagacttaga
    10321 agtatatgta cacagaaatg agactatata tcatcaagca tataaaaaag gtgtacctca
    10381 atttgactta aaagaagttg gcacaactga taagacaggt actgtcattc gttttaaagc
    10441 agatggagaa atcttcacag agacaactgt atacaactat gaaacattac agcaacgtat
    10501 tagagagctt gctttcttaa acaaaggaat tcaaatcaca ttaagagatg aacgtgatga
    10561 agaaaacgtt agagaagact cctatcacta tgagggcggt attaaatctt atgttgagtt
    10621 attgaacgaa aataaagaac ctattcatga tgagccgatt tatattcatc aatctaaaga
    10681 tgatattgaa gtagaaattg cgattcaata taactcagga tatgccacaa atcttttaac
    10741 ttacgcaaat aacattcata cgtacgaagg tggtacgcat gaagacggat tcaaacgtgc
    10801 attaacgcgt gtcttaaata gttatggttt aagtagcaag attatgaaag aagacaaaga
    10861 tagactttct ggtgaagata cacgtgaagg tatgacagca attatatcta tcaaacatgg
    10921 tgatcctcaa ttcgaaggtc aaacgaagac aaaattaggt aattctgaag tgcgtcaagt
    10981 tgtagataaa ttattctcag agcactttga acgattttta tatgaaaatc cacaagtcgc
    11041 acgtacagtg gttgaaaaag gtattatggc ggcacgtgca cgtgttgctg cgaaaaaagc
    11101 gcgtgaagta acacgtcgta aatcagcgtt agatgtagca agtcttccag gtaaattagc
    11161 cgattgctct agtaaaagtc ctgaagaatg tgagattttc ttagtcgaag gggactctgc
    11221 cggggggtct acaaaatctg gtcgtgactc tagaacgcag gcgattttac cattacgagg
    11281 taagatatta aatgttgaaa aggcacgatt agatagaatt ttgaataaca atgaaattcg
    11341 tcaaatgatc acagcatttg gtacaggaat cggtggcgac tttgatctag cgaaagcaag
    11401 atatcacaaa atcgtcatta tgactgatgc cgatgtggat ggagcgcata ttagaacatt
    11461 gttattaaca ttcttctatc gatttatgag accgttaatt gaagcaggct atgtgtatat
    11521 tgcacagcca ccgttgtata aactgacaca aggtaaacaa aagtattatg tatacaatga
    11581 tagggaactt gataaactta aatctgaatt gaatccaaca ccaaaatggt ctattgcacg
    11641 atacaaaggt cttggagaaa tgaatgcaga tcaattatgg gaaacaacaa tgaaccctga
    11701 gcaccgcgct cttttacaag taaaacttga agatgcgatt gaagcggacc aaacatttga
    11761 aatgttaatg ggtgacgttg tagaaaaccg tagacaattt atagaagata atgcagttta
    11821 tgcaaactta gacttctaag cgctgtgaac tgaacttttg aaggaggaac tcttgatggc
    11881 tgaattacct caatcaagaa taaatgaacg aaatattacc agtgaaatgc gtgaatcatt
    11941 tttagattat gcgatgagtg ttatcgttgc tcgtgcattg ccagatgttc gtgacggttt
    12001 aaaaccagta catcgtcgta tactatatgg attaaatgaa caaggtatga caccggataa
    12061 atcatataaa aaatcagcac gtatcgttgg tgacgtaatg ggtaaatatc accctcatgg
    12121 tgactcatct atctatgaag caatggtacg tatggctcaa gatttcagtt atcgttatcc
    12181 gcttgttgat ggccaaggta actttggttc aatggatgga gatggcgcag cagcaatgcg
    12241 ttatactgaa gcacgtatga ctaaaatcac acttgaactg ttacgtgata ttaataaaga
    12301 tacaatagat tttatcgata actatgatgg taatgaaaga gagccgtcag tcttacctgc
    12361 tcgattccct aacttgttag ccaatggtgc atcaggtatc gcggtaggta tggcaacgaa
    12421 tattccacca cataacttaa cagaattgat caatggtgta cttagcttaa gtaagaatcc
    12481 tgatatttca attgctgagt taatggaaga tattgaaggt cctgatttcc caactgctgg
    12541 acttatttta ggtaagagtg gtattagacg cgcatatgaa acaggtcgtg gttcaattca
    12601 aatgcgttct cgtgcagtta ttgaagaacg tggaggcgga cgtcaacgta ttgttgtcac
    12661 tgaaattcct ttccaagtga ataaggctcg tatgattgaa aaaattgcag agcttgttcg
    12721 tgacaagaaa attgacggta ttactgattt acgtgatgaa acaagtttac gtactggtgt
    12781 gcgtgtcgtt attgatgtgc gtaaggatgc caatgctagt gtcattttaa ataacttata
    12841 caaacaaaca cctcttcaaa catcatttgg tgtgaatatg attgctctag tgaatggtag
    12901 accgaagctg attaatttaa aagaagcgtt agtacattat ttagagcatc aaaagacagt
    12961 tgttagaaga cgtacgcaat acaacttacg taaagctaaa gatcgtgccc acattttaga
    13021 agggttacgt attgcgttag atcatatcga tgaaattatt tcaacgattc gtgaatcaga
    13081 aacagataaa gtcgcaatgg aaagtttgca acaacgcttc aaactttctg aaaaacaagc
    13141 tcaagctatt ttagacatgc gtttaagacg tctaacaggt ttagagagag acaaaattga
    13201 agctgaatat aatgagttat taaattatat tagtgaatta gaagcaatct tagctgatga
    13261 agaagtgtta ttacagttag ttagagatga attgactgaa attagagatc gcttcggtga
    13321 tgatcgtcgt actgaaattc aattaggtgg atttgaagac ttagaggacg aggacttaat
    13381 tccagaagaa caaattgtaa ttactttgag ccataataac tatattaaac gtttgccggt
    13441 atctacatat cgtgctcaaa accgtggtgg tcgtggtgtt caaggtatga atacattgga
    13501 agaagatttt gtcagtcaat tggtaacttt aagtacacat gaccatgtat tgttctttac
    13561 taacaaaggt cgtgtataca aactaaaagg ttatgaagtg cctgagttat caagacagtc
    13621 taaaggtatt cctgtagtga atgctattga acttgaaaat gatgaaatca ttagtacaat
    13681 gattgctgtt aaagaccttg aaagtgaaga taacttctta gtgtttgcaa ctaaacgtgg
    13741 tgtcgttaaa cgttcagcat taagtaactt ctcaagaata aatagaaatg gtaagattgc
    13801 gatttcgttc agagaagatg atgagttaat tgcagttcgc ttaacaagtg gtcaagaaga
    13861 tatcttgatt ggtacatcac atgcatcatt aattcgattc cctgaatcaa cattacgtcc
    13921 tttaggccgt acagcaacgg gtgtgaaagg tattacactt cgtgaaggtg acgaagttgt
    13981 agggcttgat gtagctcatg caaacagtgt tgatgaagta ttagtagtta ctgaaaatgg
    14041 ttatggtaaa cgtacgccag ttaatgacta tcgtttatca aatcgtggtg gtaaaggtat
    14101 taaaacagct acgattactg agcgtaatgg taatgttgta tgtatcacta cagtaactgg
    14161 tgaagaagat ttaatgattg ttactaatgc aggtgtcatt attcgactag atgttgcaga
    14221 tatttctcaa aatggtcgtg cagcacaagg tgttcgctta attcgcttag gcgatgatca
    14281 atttgtttca acggttgcta aagtaaaaga ggatgcagac gaagaaaatg aagatgaaca
    14341 atctactgta tctgaagatg gtactgaaca acaacgtgaa gcggttgtaa atgatgaaac
    14401 accaggaaat gcaattcata ctgaagtgat tgattcagaa gtaaatgatg aagatggacg
    14461 tattgaagta agacaagatt tcatggaccg tgttgaagaa gacatacaac aatcatcaga
    14521 tgatgatgaa gaataataaa aaaataagac ttccctatat gtaggggggt cttattttta
    14581 tgctagaaag taatgctata ctatattcaa tgattagtaa tgactaactt tctaattgtt
    14641 tcattgcgta aggtatttca ttgataagtc ttgatggtgg caccacatac atatcttttg
    14701 caagattttc gccaataaaa ctatgtgtat atgtggcact cataaccgct tcttttaagt
    14761 tatcaaattg accaacaaaa cttgtaatca taccagcaag tgtatcgccc ataccaccag
    14821 tcgccattgc tgggctaccg attgtcaatt taaagtcttc atctttaaag aaaatttcag
    14881 taccgtgttt tttaagtaca acagttgcgc ctaaacgatc aactgcttca cgattacgct
    14941 catatgtctg ttcctcaata ggaataccac ttaatcgttc ccattctttg agatgcggag
    15001 taaaaatcac gcgacatgta ggtaattgtg gtttcaattt actaaagatt gtgatggcat
    15061 cgccgtctac gattaaattt tgatgcggtt gtatattttg tagtaggaat gtaatggcat
    15121 tatttccttt gaaatcaacg ccaagacctg gaccaattag tatactgtca gtcatttcaa
    15181 tcattttcgt caacattttc gtatcattaa tatcaataac catcgcttct gggcaacgag
    15241 aatgtaatgc tgaatgattt gttggatgtg tagctacagt gattaaacca ctaccgctaa
    15301 atacacatgc acgagccgct aacataatgg caccacctaa gttagcagat ccaccaatta
    15361 ataaaatttt gccataatca cctttatgtg aatcttcttt acgcttagga atgttaatag
    15421 aatttaacgt ttccatagtg atataacctc ccatgtaaaa gccttttccg aatttattca
    15481 attttaaaaa tatatagtaa cttttaacaa aatgtattat aaatttctga attcattatt
    15541 atttgtcgtt aaatacaata gaaaatacta tacctgtata tgcaattcgt caatagataa
    15601 attattaaat atgcttacaa caatcttaat atcctttaac gcactacaat agtgctctga
    15661 taataggtta taaatgtacg taaaaccatt gtttcaataa aaatgaaaac gtatacttca
    15721 agaaggatgg gttacttaat ataaacaagg gggtaacata tatgacttta tatttagatg
    15781 gtgaaacact aacaattgag tatattaaat catttttaca acaacaatca aagattgaaa
    15841 ttattgatga tgcgttagaa cgtgtcaaaa aaagtagagc agtagttgaa cgtattattg
    15901 aaaatgagga aacggtttac ggtatcacta caggttttgg gttatttagt gatgtacgta
    15961 tagacccgac gcaatataac gaattacaaa tgaatctgat acgctcacat gcctgtggac
    16021 taggtgagcc attttcaaaa gaagtagcat tagtcatgat gattttacga ttgaatacat
    16081 tattaaaagg tcattcaggt gccactttag aattagtgag acaattacaa ttttttataa
    16141 atgaacgtat tataccgata atcccacagc aaggctctct cggtgcatca ggagatttag
    16201 cgccattatc acatttagca ttagcattaa ttggtgaagg gaaagtattg tacagaggtg
    16261 aagaaaagga tagtgacgat gtattaagag aattaaatag acaacctttg aaccttcagg
    16321 ctaaagaagg tttagcattg attaatggta cgcaagctat gacagctcaa ggtgtcatta
    16381 gttatataga agcagaagat ttaggttacc aatctgaatg gattgctgca ttaacgcatc
    16441 agtctcttaa tggcattata gatgcatatc gacatgatgt gcacgctgtt cgtaattttc
    16501 aagaacagat taatgtggca gcgcgtatgc gtgattggtt agaaggatca acattaacga
    16561 cgcgacaagc agaaatacgt gtacaagatg catatacatt gcgttgtata ccacaaatcc
    16621 atggcgcgag ttttcaagta ttcaattatg ttaaacagca attagaattt gaaatgaatg
    16681 cggctaatga taatccactt atatttgagg aagcaaatgc aacgtttgtt atttcgggtg
    16741 gtaacttcca tggacaacct attgcttttg cattagatca tcttaaatta ggtgtaagtg
    16801 aattagcaaa cgtatcggaa cgtcgtctag agcgactagt aaatcctcaa ttaaatggtg
    16861 atttaccagc atttcttagt ccagagccag gattgcaaag tggcgcgatg attatgcaat
    16921 atgctgctgc aagtctcgtt tctgaaaata aaactttagc gcatccagcg agtgttgatt
    16981 ctatcacttc atctgcgaac caagaagatc acgtatctat gggaactaca gctgctagac
    17041 atggttatca aattattgaa aatgcaagac gtgtgttggc aatcgaatgt gttattgcat
    17101 tacaagcagc agagttgaaa ggtgtcgaag gattatcacc aaaaacacgt cgcaagtatg
    17161 atgagtttcg aagtatcgtg ccatccatta catatgaccg tcaatttcat aaagatattg
    17221 aagcggttgc acagtattta aagcaatcaa tttatcaaac gactgcatgt cactaaatcg
    17281 acatgattga tgtttgaaat cgttacttga aaaagcaaat atagtttgct atattaaaaa
    17341 taacttaata agacattgtt cgtaggacaa gtaatatata gtgttcgata tcagagagct
    17401 tgtggttagt gtgaacaaga atcaacatat atatgaatct acctacttat ttaaaagaac
    17461 aatcggtgat aaccgttatt ttagtgaagt gcaatttagg tttagtgtat ctttataact
    17521 taaattgtta aatagggtgg caacgcgtag accacgtccc ttgtagggat gtggtctttt
    17581 tttattttct aaaaaataaa gacgtgcgtc attccataat aaatgataat tatttgggaa
    17641 aggatgaagg ttaatgttag acattagatt attcagaaat gagcctgaca cagttaagag
    17701 caaaattgaa ttacgcggag atgatccaaa agttgtagat gaaattttag aattggatga
    17761 gcaacgacgt aaattaatta gtgcaacaga agaaatgaaa gcacgtcgta ataaagtaag
    17821 cgaagaaatc gcattaaaaa aacgtaataa agaaaatgct gatgatgtga ttgctgaaat
    17881 gcgcacatta ggtgacgata ttaaagaaaa agatagtcaa ttaaatgaaa ttgatagtaa
    17941 aatgacaggt atcctttgtc gtattccaaa tttaataagt gatgatgtac ctcaaggtga
    18001 atctgatgaa gataacgttg aagttaaaaa gtggggtaca ccacgtgagt tttcatttga
    18061 accaaaagca cattgggata ttgtagaaga attgaaaatg gctgattttg atcgtgcagc
    18121 aaaagtttca ggtgcgcgtt ttgtatattt aacaaatgaa ggtgcgcaat tagagcgtgc
    18181 tttaatgaac tatatgatta caaaacatac aacacaacat ggttatacag aaatgatggt
    18241 accacagctt gtaaacgcag atacaatgta tggtacaggt caattaccta aatttgaaga
    18301 agatttattt aaagtagaaa aagaaggatt atatacaatt ccaactgctg aagtaccatt
    18361 aacgaatttc tatcgtaatg aaattattca accaggtgta cttcctgaaa aattcactgg
    18421 tcaatctgca tgtttccgta gtgaagcagg atcagcaggt agagatacaa gaggattaat
    18481 tcgtttacat caattcgata aagtggaaat ggtacgtttt gaacaacctg aagattcatg
    18541 gaatgc
//
LOCUS       DAMZPP010000046        18474 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMZPP010000046 DAMZPP010000000
VERSION     DAMZPP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18474
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.046"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..662
                     /locus_tag="POH73_001199"
     CDS             <1..662
                     /locus_tag="POH73_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HDI6887164.1"
                     /translation="EVSKSLTEDLLTLRSYLIEQNYPFIIEIVPSESDMMIVYDARDM
                     IKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYGSKYGPDLESLLKHYKIK
                     LETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSKQKKFIPAGSVVLEGKKC
                     GIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFRPINENELEVGAFKDVNH
                     N"
     gene            646..1650
                     /locus_tag="POH73_001200"
     CDS             646..1650
                     /locus_tag="POH73_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDI6887165.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEQMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            2124..4064
                     /gene="ltaS"
                     /locus_tag="POH73_001201"
     CDS             2124..4064
                     /gene="ltaS"
                     /locus_tag="POH73_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HDI6887166.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
     gene            4336..6219
                     /locus_tag="POH73_001202"
     CDS             4336..6219
                     /locus_tag="POH73_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDI6887167.1"
                     /translation="MGMEAYKIEHLNKSYADKIIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAERELTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LFEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREESEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQHSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            6231..8012
                     /gene="recQ"
                     /locus_tag="POH73_001203"
     CDS             6231..8012
                     /gene="recQ"
                     /locus_tag="POH73_001203"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDI6887168.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            8233..9210
                     /locus_tag="POH73_001204"
     CDS             8233..9210
                     /locus_tag="POH73_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6887169.1"
                     /translation="MIKFKNVTKRYDKHVAVDNISFDINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQQIEVLYQHLSESERVIVMQEDGVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            9203..10717
                     /locus_tag="POH73_001205"
     CDS             9203..10717
                     /locus_tag="POH73_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDI6887170.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYDQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKIKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            10957..12015
                     /gene="hisC"
                     /locus_tag="POH73_001206"
     CDS             10957..12015
                     /gene="hisC"
                     /locus_tag="POH73_001206"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDI6887171.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLDGILKEVNDDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPSHVPVLIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEHFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNGKMLEVLSNFKYE"
     gene            12174..12716
                     /locus_tag="POH73_001207"
     CDS             12174..12716
                     /locus_tag="POH73_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HDI6887172.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLREPGFFRHLKVMPHAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(13211..14128)
                     /locus_tag="POH73_001208"
     CDS             complement(13211..14128)
                     /locus_tag="POH73_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDI6887173.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKAKDYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILSANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPTMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            14219..14320
                     /locus_tag="POH73_001209"
     CDS             14219..14320
                     /locus_tag="POH73_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887174.1"
                     /translation="MVITEIDVYRVGRENIVIVVYNEHIKFLFILIS"
     gene            complement(14398..15903)
                     /locus_tag="POH73_001210"
     CDS             complement(14398..15903)
                     /locus_tag="POH73_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDI6887175.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVILAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAFSPLIIKAMKGIR"
     gene            complement(16243..16743)
                     /gene="queF"
                     /locus_tag="POH73_001211"
     CDS             complement(16243..16743)
                     /gene="queF"
                     /locus_tag="POH73_001211"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDI6887176.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEQMAEHRLMNHDLYP
                     EKIDNR"
     gene            complement(16763..17629)
                     /locus_tag="POH73_001212"
     CDS             complement(16763..17629)
                     /locus_tag="POH73_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI6887177.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGKLSSQPLLLTRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQTQVLYLLGAGLAAAAG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKVIKK"
BASE COUNT         6784 a         2624 c         3195 g         5871 t
ORIGIN      
        1 aagaagtatc taaaagttta accgaagatt tattaacact tcgctcatat ttaattgaac
       61 aaaattatcc atttattata gaaattgtgc catcagaatc agacatgatg attgtctacg
      121 acgcaaggga tatgattaaa caccataata tacaatcacc ttttttatac atgaaagcac
      181 taatagaatc gattcattta aacataaaac atgattttaa ccagcaagat ttgattgaaa
      241 taccaattgt gtatggttcg aaatatggtc cggatttaga atcactttta aaacattaca
      301 aaatcaagct agaaacattt attgaattac attctaaggc gcaatatttt gtttcgatga
      361 tgggatattc acctgggttt ccttatttaa ctggattaaa taagaaattg tatattaatc
      421 acacgagtaa acagaaaaaa ttcattccag ctggttctgt agtacttgaa gggaaaaaat
      481 gcggtattgt aactacggat acaattaatg attggttagt tattggttat acaccattat
      541 cactttttaa tccgaaagaa tcagatttcg cacgcttaaa gttaggcgat aatattaaat
      601 ttagacctat caatgaaaat gaattagaag taggagcgtt taaagatgtc aatcataatt
      661 gaaaaaagtg gcttattcag tagctttcag gactttggta gaaggggata tgaacatgat
      721 ggtgtaattc catgtggtgc acttgatact ttagcacatg aaattgctaa tcgattagtt
      781 gcaaatgaca agaatgaagc aactttggaa atgactaata aaatggcaac gattcgtttt
      841 acagaaccta cgctgattgc attagcaggg ggtaatgtca aagcttacac tgagcaaatg
      901 actatatctc catataaatt gtatttgtta gataaaggcg atgttttaaa gtttagagaa
      961 acaagttata catcgcgagt gtatttagct gtgggaggtg gatttgaatt agatgcatgg
     1021 ttaggatcta attcaaccga ctttaatgta aaaattggtg gttttaaagg aagaacatta
     1081 caagatggcg atgaaataaa gcttaagaga gattatacag ctcgtcatca taagttattt
     1141 gaaaaccttg ctcacacgaa acaaacagat tggggtattg atgggtacgc cttgtcattt
     1201 aattatatgt ctgatgtatt tcatgtcgtt aaaaataaag gtacggaaga ttttaaagaa
     1261 gatgccattc aaagatttgt gaaacatgat tataaagtaa cgagcaaagc aaatcgcatg
     1321 gggatgatgc ttgaaggtga aaaaatcaaa gctttttatg aagatatgcc accgtatcag
     1381 actgtcaaaa aaggaacgat acaaattaag cgtgatggca cacctattat cttattaaat
     1441 gatcattaca cgttaggtag ctacccgcaa atcggtacaa tcgcaagtta tcatttaacg
     1501 aaattagcac aaaaaccgca aggatcacgt ttgaaatttc aatttataga tattttaacg
     1561 gctgaaaaga acctcgttaa gtatagtaac tggttaaacc aattattcca tggaatagaa
     1621 tatagaatgc aattagaaat gatgaaataa tatttggtac gtagctcata ctagagtccg
     1681 atgcaaataa tttcctccta atgtataatg aaataatact gtgttttatc tgcgaaatgt
     1741 attatattct aattcgtttc acagtaaaat gaaaagataa agtgtgtttt tacttgaatt
     1801 ttgactaaaa ttactctata tttattaatt gagctatgct tattattaca atttgattac
     1861 aaattttaaa tttgttaatt gaatgataat attaaataaa gaaacttaca caagcaaata
     1921 tgagttgtag cccaaaatac ttgttaaatc aaagttgaaa gctacaaata atgaaaatta
     1981 taaacttgaa tctgaaagta attactataa ttatgacaat gttaactttt aaacgcactt
     2041 attaattaac tacataatgt taatatctaa tttattcaag tactttcgca agatttatta
     2101 tctaaataac gggggaaaga atcatgagtt cacaaaaaaa gaaaattagt ctttttgcgt
     2161 tcttcttatt aaccgtaata acgattacct tgaagacgta tttttcttat tatgttgatt
     2221 tttctttagg tgttaaaggt ttagtacaaa acttaatatt attgatgaat ccttatagtt
     2281 tagtagcact ggttttaagt gtgttcctat tctttaaagg caaaaaagca ttttggttta
     2341 tgttcatagg cggcttctta ttgacgttcc tattatatgc caatgttgtg tactttagat
     2401 tcttctcgga ttttttaacg tttagtactt taaaccaagt aggtaacgta gaatctatgg
     2461 gtggtgcggt tagtgcatca ttcaaatggt atgactttgt ttatttcatt gatacgttag
     2521 tttacttatt cattttaata tttaaaacaa aatggttaga cacaaaagca tttagtaaga
     2581 aatttgttcc tgtcgtaatg gcggcttcag tagcattatt cttcttaaac ttagcttttg
     2641 ctgaaactga cagaccagaa ttattaacac gtacatttga ccataaatat ttagtgaaat
     2701 atttaggacc gtataacttt acagtatacg atggtgttaa aactatcgaa aataatcaac
     2761 aaaaagcgtt agcatctgaa gatgacttaa caaaagtatt aaattatacg aaacaacgtc
     2821 aaacagagcc taacccagaa tattatgggg tagcaaagaa gaaaaatatt attaagattc
     2881 acttagaaag tttccaaacc ttcttaatta ataaaaaggt taatggtaaa gaagtaacac
     2941 cgtttttaaa caaattatca agtgggaaag agcaattcac atacttccct aactttttcc
     3001 atcaaacagg tcaaggtaaa acatctgact ctgaatttac aatggataac agtttatacg
     3061 gtttaccgca aggttctgcc ttttcattaa aaggagataa tacgtatcag tcattaccag
     3121 caattttaga tcaaaagcaa ggctacaaat ctgatgtcat gcacggtgac tataaaacat
     3181 tctggaacag agaccaagta tataaacact ttggtatcga taaattctat gatgcaacat
     3241 actatgacat gtcagataaa aacgttgtaa acttaggctt gaaagacaaa attttcttta
     3301 aagattctgc taattatcaa gctaagatga aatcaccatt ctattctcat ttaattacat
     3361 tgactaacca ctatccattc acattagatg aaaaggatgc aactattgag aagtcaaaca
     3421 caggtgatgc aacagttgat ggttatattc aaacagcacg ttatttagac gaagcattag
     3481 aagaatatat taatgacttg aagaaaaaag gattatatga caattcagtg attatgattt
     3541 atggtgacca ctatggtatc tctgaaaacc ataacaatgc catggaaaaa ctattaggtg
     3601 aaaaaatcac accagctaaa tttacagatt taaacagaac tggtttctgg attaaaatcc
     3661 ctggtaaatc tggtggtatc aataatgaat atgctggtca agtcgatgta atgccaacaa
     3721 ttttacattt ggctggtata gatacgaaga actatttaat gttcggtact gatttattct
     3781 ctaaaggtca taatcaagta gttccattca gaaatggtga ctttataaca aaagattata
     3841 aatatgttaa tggtaagatt tattctaata aaaataatga actcataact actcaaccag
     3901 ctgatttcga aaagaataaa aagcaagttg aaaaggatct cgaaatgagt gacaacgtgc
     3961 ttaatggtga tttatttaga ttctacaaaa atccagactt caaaaaggta aatccttcga
     4021 agtataaata tgaaacagga cctaaagcaa actctaaaaa ataatattta aacatgaacc
     4081 cggattgata aaatatcaat ccgggttttt atttatgttc ttttatatta tgttttacat
     4141 tatatgtgtt gataaaaagg tatattataa aaatcattag catagtcatc aaagccttat
     4201 caagttttat ttagagttga agcaacaata tattaaatta tgacacttaa cagtatcgat
     4261 ttcttttgat ttcaaaaagg tgtataatgt atagaactga tttaagtacg tagagatttc
     4321 aacagaagaa ggaagatggg tatggaagca tataaaattg aacatttaaa taaatcttat
     4381 gccgataaga ttatattcga taatctagat ttatcaattt cagaaggtga aaaaataggt
     4441 ttagtaggca taaatggtac agggaaaagt acgttgttaa aagtaattgg tggtattgat
     4501 gatgatttta cagccaatgt tatgcatcca aatcaatatc gaattcgtta ttcgtctcag
     4561 aaacaggatc ttaatgaaga tatgactgtt tttgatgcag tattaagttc tgatacaaca
     4621 actttacgca tcatcaagca atatgaacag gcagtacaag cttatgcgga tgatcaaagt
     4681 gataaattgt tcaagcgaat gatggatgcg caagatgcta tggatcaaca tgatgcttgg
     4741 gactataacg ctgaaattaa aacgatcctc tcaaaactag gtatacatga tactactaaa
     4801 tacattaaag aattatccgg cggacaacaa aaacgtgttg tacttgctaa aacattaata
     4861 gaacaaccag atttattgtt attagatgaa cctacgaacc atttagactt cgaatcaatc
     4921 agctggttga tcaattatgt gaagcaatat cctcatacag ttttattcgt aacccatgat
     4981 cgatattttt taaatgaagt ttccactaga attattgaac taaacagagg taagttagcg
     5041 tcatatcctg gtaactatga atcttatatt gaaatgcgcg ctgaaagaga actaacactt
     5101 caaaagcaac aacaaaagca acgagcttta tataaggaag aacttgcttg gatgagggct
     5161 ggagctaagg cacgtactac aaagcaacaa gctagaatta atcgatttaa tgacctagaa
     5221 aatgaagtta accagcaata taaagacgat aaaggtgaat tgaatcttgc ttattcaaga
     5281 ttaggtaagc aagtgttcga attagaagac ttatcaaagg ctattaatga taaagtatta
     5341 tttgaacatc tgacggaaat tattcaaaaa ggtgagcgta ttggtgttgt tgggccaaat
     5401 ggagctggta aaacaacact cttaaatatt ttgagtggag aagaccaaca attcgaaggt
     5461 aaattgaaga ctgggcagac ggttaaagta gcttatttta agcaaacaga tgagaccctg
     5521 gatagagata ttcgtatgat tgattattta agagaagaaa gtgagattgc aaaagaaaaa
     5581 gatggaacct cggtatctat tacacaactt cttgaacgat ttttatttcc aagtgcaact
     5641 catggtaaaa aagtttataa attatctggt ggagagcaaa agcgtttgta tttattacgt
     5701 ctactcgtac accagccaaa tgttctgttg ttagatgaac cgacaaatga tttagatact
     5761 gagactttaa caatacttga agattatatt catactttcg gtggtacagt gattaccgta
     5821 agccatgatc gctacttctt aaataaagtt gcacagtcat attggtttat tcatgatggt
     5881 cagatggaaa agattatcgg aacttttgaa gattatgaaa gttataaaaa atcattagat
     5941 aaaaataaat ccacattgaa gcaacactct aaatcttcta caactgtacg taagaaaaat
     6001 ggtttatcat ataaagaaaa attagaatat gaacaattga tgaaacgcat agaacaagcg
     6061 gaagtaagaa tggaagaaat tgatgtgctc atgattgagg caagtgcaga ttatgggaaa
     6121 attaaagaat taaacgaaga aaaagaacaa cttgaaattc aatatgattt agacatcaca
     6181 agatggagtg agttagaaga aattaaagaa caacaataag gggtcaattt atgatgcaac
     6241 aaacattatc gcattacttt gggtatgaaa cgtttcgacc aggacaagaa gaaattatta
     6301 gcaaagtatt agaccatcgt aatgtgcttg gtgtcttacc aactggtgga ggtaagtcta
     6361 tatgctatca agtaccaggt ttattgttag gtggtacaac aattgtaata agtccactaa
     6421 tatcattaat gaaagatcaa gtggatcaat taaaagcgat gggaattcaa gctgcttttt
     6481 taaatagtag tttgactcaa aaagagcaac aacgtattga aaaagcatta tcaaatggag
     6541 aaattcaatt tttgtatgtt gcaccagaac gatttgaaaa ccgatatttt ttaaatatgc
     6601 ttcagcgtat aaagattcat ttagtcgcgt ttgatgaagc gcattgtata tctaaatggg
     6661 gacatgattt tagaccgagt tatcaaaacg ttatttctaa agtatttacg ttacctcaag
     6721 attttacaat aatagcgttg acagcaactg ccacggttga agtacagcaa gatattagag
     6781 aaaagttaaa tatcgctcaa actgatcaaa ttaaaacgag tactaagcgt agaaacttaa
     6841 tttttaaagt aaatcctact tatcaacgtc aaaaatttat attggattat attaaaacac
     6901 acgatgaaga tgcaggcatt atttattgtt ctacacgtaa gcaagttgaa gagcttcaag
     6961 aagccttaga aagccagaaa attgaaagtg ttatatatca tgcaggtttg agcaataaag
     7021 aaagagaaga agcgcagaat gatttcttat ttgatcgtgt taaagtagtc gttgctacaa
     7081 atgcttttgg catgggtatt gataaatcca atgtacgttt tgttattcat tataatatgc
     7141 ctggagattt agaatcttat tatcaagaag cgggtcgtgc aggtcgtgat gggttgaaaa
     7201 gtgaatgtat tttgttattt agtgaacgcg atatcaattt acacgagtat tttataacag
     7261 tctctcaagc tgatgatgac tataaagata aaatgggcga aaagttaact aaaatgattc
     7321 aatatacaaa aacaaaaaaa tgtttagaag caacaattgt ccattatttt gaaccgaatg
     7381 aaaaattaga agaatgtgaa caatgtagta attgtgttca acaagataaa tcatataata
     7441 tgacacaaga agctaagatg attattagtt gcatcgctcg tatgaaacaa caagagagtt
     7501 atagtgttat cattcaagtg ctaagaggag agtcaacaga ttatattaag tataaaggtt
     7561 atgatcaaat ttcaacccat ggtttaatga aaggctacac aacatcagaa ttaagtcact
     7621 taatagatga attaagattc aaagggttct taaatgaaaa tgatgaaata ttaatgtgtg
     7681 atacttcaat taaaaaatta ctcagtaatg aagtagaagt attcacaaca ccatttaagc
     7741 aaaaagcgac tgaaaaagta tttataaata cggttgaagg ggttgaccga gtattattca
     7801 gtcaattggt agaagttcgt aaaaagttaa gtgacaaatt aacgatagca cctgtaagta
     7861 tattttctga ttacacgttg gaggaatttg ctaaacgtaa gcctgcttcg aaacaagata
     7921 tgattaatat tgatggcgta ggtagttata aattaaaaca ttattgtcca gcatttttag
     7981 aaacgattca aaattataaa gccaaagtat aatgaaggca cctagtaaac attccttgtt
     8041 ttactgggtg ttttttgaat ttaaagtaaa ataacactat ttcttaactt gagattacct
     8101 attattcatt attttgttaa aaaagttcaa ggataaatca tttaaatttg aaaattgtaa
     8161 taaacgtggt taaataggga atacatacat ataatctcgg ttttatgcta tgagaaaaaa
     8221 agaggtggca aagtgattaa gtttaaaaat gtaactaagc gttatgacaa acatgttgct
     8281 gtcgataaca ttagtttcga tattaatgag ggtgaattct ttgtactaat tggaccttca
     8341 ggttgtggaa aaactacgac attaaaaatg attaatcgac tcattcactt aagtgaaggt
     8401 tatatttatt ttaaagataa accaataagt gattatccag tatacgaaat gcgttgggat
     8461 attggatacg tattgcagca gattgcatta ttcccacata tgacaatcaa agaaaatatt
     8521 gcacaagtgc cacaaatgaa aaagtggaaa gaaaaagata tagataaaag agtagatgaa
     8581 ttacttgaaa tggttggatt agaacctgaa aaatataaaa acagaaaacc tgatgaattg
     8641 tcagggggac aacgacaacg tgtaggagtt atacgtgcgt tagcagctga tccaccagtt
     8701 attttaatgg atgaaccgtt tagtgcatta gacccaatca gccgagaaaa acttcaagat
     8761 gatttaattg aattacaaac taaaattaag aagacaatca tatttgttac acatgatatt
     8821 caagaggcga tgaaacttgg tgataagatt tgtcttttga atgaaggtca tattgaacaa
     8881 attgacacac cagaaggatt taaaaataat cctcaaagtg aatttgttaa acaatttatg
     8941 ggtagtcatt tagaagatga tgcgccatgt gttgaagaga atgcgattat ccgtgacttg
     9001 gatattatga aaccaatcga tgaggttaca tctatgagcg cttatccaat tgtttatgac
     9061 aatcaacaaa ttgaagtatt gtatcaacat ttatcagaga gcgagcgtgt cattgtcatg
     9121 caagaagatg gcgtaggtca atatgttatt gataggaaag atatcttcaa atatttgtcc
     9181 cagaaaaagg aggtagctca acatgactaa ctttttcgac atattgagtg agcgtaaggg
     9241 gcaactattt tcgacaatga tagaacatat tcaaatatca tttatcgcat tattgattgc
     9301 aactgctatt gcggtaccat taggtatttt attgacgaag actaaaacga tatctgaaat
     9361 cgtaatgaat attgcggcaa ttcttcaaac cataccatcg ttggcattat taggtttaat
     9421 gattccttta tttggtatcg gtcgtgtgcc agcaattatc gcacttgtag tgtatgcatt
     9481 gttaccaatt ttaaggaata cgtatactgg aattaaagaa gttgatccat cgctcattga
     9541 agcggctaaa ggtataggta tgaagccatt tagacgttta actaaagttg aacttccaat
     9601 agcaatgcct gttataatgg ctggtgtaag aacggctatg gtattaatta taggtacagc
     9661 aacactagca gcattaattg gtgcaggcgg actaggagat ttaattttat taggtataga
     9721 ccgtaacaat gcatcattga tattattagg tgcaattcca gcagccttat tggcaattat
     9781 atttgattta attttaagat ttatggctaa attatcttat aaaaagttat tgatgacgtt
     9841 aggtgttata gtgatgatta ttatactggc catcgctatt cctatgtttg cacaaaaagg
     9901 tgataaaatt acgttagctg gaaagcttgg ctccgagcct tcgattatta caaatatgta
     9961 taaaatttta atagaagaag agaccaaaaa tactgtagaa gtgaaagatg gtatgggcaa
    10021 aacagcattt ttatttaatg ctttaaaatc tgacgatata gatgggtatt tagagtttac
    10081 tggaacagtt ttaggtgaat taacaaaaga accattgaag tcaaaagaag agaaaaaagt
    10141 atatgatcaa gctaagcaaa gtcttgaaaa gaaatatcaa atgactatgt taaaaccaat
    10201 gaaatataac aatacgtatg ctttagctgt aaaacgtgat tttgctaaac aacataatat
    10261 acgtacaatt ggtgatttaa ataagattaa agatcaactt aaaccaggat ttacattgga
    10321 atttaatgat cgtccagatg ggtacaaagc tgttcaaaag gcttataatt taaatttaga
    10381 taacatacgt acaatggaac ctaagttgag atatcaagcg attaataaag gtaatattaa
    10441 tttaattgat gcatattcaa ctgacgctga attaaaacaa tatgatatgg ttgtgttaaa
    10501 agatgataag cacgtatttc caccatatca aggagcacca ttatttaaag aaagcttttt
    10561 aaagaaacat ccagaaatta agaaaccgtt aaacaaacta gaaaacaaaa tatctgatga
    10621 agatatgcaa atgatgaact ataaagtaac agttaaaaat gaagacccat atacagttgc
    10681 gaaagattat ttaaaagcaa aagggttaat caaataacga ccaacgccac ataagatgag
    10741 gggcacgcca ttatatctta tgtggcgttg ctatatttac atctatgttt atgttcaatt
    10801 taaacatgca atactgatta aaaaatatga catgttaaac acaatgtaag ctagtaatgt
    10861 gtgaaaatag tagcattgca ttttagaaac atagagcgat ataatgaata taagtttttt
    10921 aaaatttcag ttaattctaa ggaggttgtt tttattatga aagaacaact taatcaacta
    10981 tcagcatatc agcctggttt atcaccaagg gcattgaaag aaaagtatgg cattgaagga
    11041 gatttatata aacttgcatc aaatgaaaat ttgtatggac catcgcctaa agttaaagaa
    11101 gcgatatcag cacacttaga tgagttatat tattatcctg aaacaggatc accgacatta
    11161 aaagcggcga ttagtaaaca tttaaatgta gatcaatcac gcattttatt tggtgcggga
    11221 ttagatgaag ttatattaat gatttctaga gcagtattaa cgccagggga cactattgtt
    11281 acaagtgaag cgacattcgg tcaatattat cacaatgcga ttgttgaatc agctaatgtg
    11341 atacaggtac ctttaaaaga tggtggcttc gacttagatg gcattttaaa agaagttaat
    11401 gatgacacgt cattggtatg gttatgtaat ccaaataatc ctacaggtac gtattttaat
    11461 catgagagct tagattcgtt tttatctcaa gtaccatcac atgttccggt actcatagat
    11521 gaagcttatt ttgaatttgt gacagcagag gactacccgg atacacttgc tttgcaacaa
    11581 aaatatgaca atgcattctt attacgtaca ttttcaaagg cgtatggatt agcgggttta
    11641 cgtgtaggat atgtggtagc aagcgaacat gcgattgaaa aatggaacat cattagacca
    11701 ccatttaatg tgacacgtat atctgaatac gcagcagttg cagcacttga agatcaacaa
    11761 tatttaaaag aggtaacaca taaaaatagt gttgaacgtg aaagatttta tcaattacct
    11821 caaagtgagc atttcttacc aagtcaaacg aattttatat ttgtaaaaac aaagcgggta
    11881 aatgaacttt atgaagcact tttaaatgta gggtgtatta cgcgaccatt tccaactggt
    11941 gttagaatta caattggttt taaagaacaa aatggtaaaa tgttagaagt tttatcaaac
    12001 tttaaatacg aatagtaagt gggcaccaca tggtatgaat atgtgacaaa atgcagtgtg
    12061 aatgatgagg tgagagtatt cagtgtaaaa agcaacaata gatgatattg ttttgtatca
    12121 attgcttttt tgctatactg aatcaatact gatattttga ggagaagatt aaaatgaccc
    12181 gtaaatcaat cgcgattgat atggatgaag tattggcaga tacattagga gaaatcattg
    12241 atgctgtcaa ttttagagcg gatttaggta ttaaaatgga agctttgaat ggtcaaaaac
    12301 ttaaacatgt tattcctgaa catgatggat taattacaga agtattaaga gaaccaggct
    12361 tcttcagaca ccttaaagtg atgccgcatg cacaagaagt tgtaaaaaaa ttaactgaac
    12421 attatgatgt atatattgct acagcagcaa tggatgtacc aacatcattc agtgataaat
    12481 atgaatggtt actagagttc tttccattct tagatccaca gcattttgtt ttttgtggta
    12541 gaaaaaacat cgttaaagct gattatttaa tagatgacaa tcctagacag cttgaaattt
    12601 ttactggtac accgattatg tttacagcag tgcataatat taatgatgat cgatttgaac
    12661 gcgtaaatag ctggaaagat gtagaacagt attttttaga taatattgag aaataaaaat
    12721 atgtcactcg aagcctttca tgtagcaaat aaaatgcata tactggggcc ccaacataaa
    12781 gaaatacttt ttctttagaa attagtattt cttatgcatg agtgtaactc atgcaatcct
    12841 atttttaagt acacattagc tgtgactaat gataaagaat cgctacataa tcaaccatta
    12901 gtcgttcttt atcatttcca tcccactctc aataaatgtt agtctatctc attattataa
    12961 atcggatgaa tgtgttaatc tatggcagat tacacttcat ccgatttttt atagaatttg
    13021 aaaaaggcac ataaaccact atgatttaaa atacaacatc aatcatttta gtggcatgcg
    13081 ccaaaattat atgtctgttt ttgaaacagg gtaatagctt aaagctaata ccaacaaata
    13141 ttacgtacgt tgaatcttat gactacactt taaacctaat ataatatcgc gttaatatca
    13201 ttccggatgc ttacaaatca ttgacagtaa gcaactgaat ggcatttggt ataacctcaa
    13261 tatcaatagg tgtttctaat gaaatttcgc catcaatatc aactttcatt gttggatctg
    13321 ttgtaagtga aatcttttta ccaggtatat gctcaatacc ttgagtaatt tcattccaat
    13381 tcatactatc acgcttttta aaaatatcat ttaaaatact gaaactttgt tcattaaaaa
    13441 tgaaagtgtt cagttcacca tcttgaggag acaaatcagt caatggtatt cgactaccac
    13501 caatgaatgg accatttgct gatagtatca tggtcgtttc gccagaatat gtcttatcat
    13561 caattgataa ttgataatta aattgtgttg gatttagcag tgttttgaca gttgatccaa
    13621 tataactcaa tttaccaaat atatcttttg aaccatcttg tacgttttca gcgttttgaa
    13681 caattagacc taagccaaca aagttgagtg catattgatt atttatttta attacatcgt
    13741 atgtaccaac ttgtgcagaa atcatttgtt cactagcttg tttatgatta ggtgctatat
    13801 ttagcgtttt tgtaaaatca ttaaaagtac cgcctggtaa aatgccaata gggagttgaa
    13861 ggtcgtgtgt cataacacca tttataagtt cgttaaccgt gccatcaccg ccaagaataa
    13921 ataatatatc tacatctttt gcataatctt tagctttaat ttcttggcaa tatttaatta
    13981 tgtcaccttc gttttcactc aattgaatag aaagatgctt acaaattgaa cttaatgccg
    14041 ttgtaacttc cccgatacct tgattaatat tttttaatcc actgtgttca tggtaaaaga
    14101 ggacaccatg tgtatattta ttttccatag tttagcctac tttctaaaaa ttggttcatt
    14161 aaatatatat acccactttt aattgttaat accaaaaata tgtttttaaa tagagaaaat
    14221 ggtaataact gaaattgatg tctatagagt gggacgagaa aatatagtta tagttgtcta
    14281 taacgagcat attaagtttt tatttatact gatatcttga atttaattat tagaaaccaa
    14341 taaaaaaaca gtaagccatt taaatgactt actgtttttt gaattaggcc aacaatatta
    14401 acgaatacct ttcattgctt taataattaa aggtgagaat gctaatacaa ttgttgtaac
    14461 aataattgca acaacaccta agaagataaa gtaatttgtt tgacctagtg gttctattaa
    14521 cttaactaat gtaccattga ttgcttgtgc agaagcgtta gttaagtacc aaatactcat
    14581 catttgggca ttaaatgctt taggtgctaa cttaacagca gcactattac ccgttggtga
    14641 taagcaaagt tcaccgataa cacagataat gtacgataaa ataacccagt taactgagaa
    14701 gtttgatgaa cctgatgcat aacctacaat accaattagt atgtatgacg cacctgctaa
    14761 gaacgtacca attgcaaatt tcactggcag gctaggttgt ttagttccaa gcttttgcca
    14821 taaaagtgaa ataattggag ctaataataa aataaataat gggttaattg attggaaaat
    14881 cgcttcacca aagtttgttt tccaaccaaa taagtttaat ttcatatctg aatgttcaat
    14941 tccataaatg ttcaatacat tagacccttg ttcttgaata gcccagaaca ccattccaag
    15001 aataaataat ggaataaatg ctttaacacg agaacgttca gtatcagtga catctttact
    15061 tctaataatt aaagtgaagt aaatgattgg taatgcaata cctaatacta aaacagtatt
    15121 actaactaag ttaaatgata atgagttagt taatgcacca ataacgataa ttaatacaat
    15181 tgctaagata acacttccga taataagacc atactttttc ttttcagctg gtgtcaatgg
    15241 gttagtaggt ttcataccaa cgctacctaa gtttttgcgg ttgaaaagta cataccatac
    15301 taaacctaat gccataccaa cagctgcaat caagaatcca ccgtggaagt ttttaacatt
    15361 aacaaagtgt tgcaaaataa taggtgataa taatgcaccc atattaactg acatatagaa
    15421 aataacaaaa cctgcatcca tacgtctatc attttcagga tataaacggc caacgatatt
    15481 tgaaatgttt ggcttcatta aacctgaacc aataatgatg aagaacattg atgtgaataa
    15541 gccgattaat gcaaatggta agcttaaaca aatatgtccg ataataataa agactgcacc
    15601 taataaagta gcgcctctag tacctgtaat tctgtcagca atccatccgc ctggtattga
    15661 tgtcatatag attaatgaac cataaactga cataattgac atagctgttg ttttatcaat
    15721 tccaaggcca ttatctgtta cggcaaagta catgtagaaa atgagtaggg cacgcatgcc
    15781 ataataacta aacctttccc agaactctac aaagaagagt acgcctagcc ctcgaggatg
    15841 cccgaaaaat cctgtttgag gtatgtcttg aatttgattt ccatgggagt tttgttgtgt
    15901 catttataca tcccatcctt tcttccccta atacaatagt ttaagtaaat ttgattgtgc
    15961 cttatcaact aaatatttca caaatcacaa aaaaataaaa atattgagaa tattctgtta
    16021 ttaagcgcgc acaattaatt agagataata taaaaatata cttattgacg ttaaagtgca
    16081 ataggtttct ataaaaatgt tgtttaattc gtgtactaat attcttttgg tgtactttgg
    16141 tataaaatgt tattttaatg taacctatta tgtatgctaa atgaaaaaca caccaataag
    16201 ttaggaaaat caacttattg gtgtgtttat taaatgatac atttaacgat tatctatttt
    16261 ttcgggatat aaatcatgat tcattaaacg atgttcagcc atttgttcgt atttagaatt
    16321 tggtcgtcca tagtttgtat aagggtcaat agaaattcca ccacgtggtg tgaacttgcc
    16381 ccaaacttca atataatgtg gatccataag ctctatcaag tcattcataa ttatattcat
    16441 acaatcttca tggaaatcac cgtgatttct gaaactaaat aagtaaagtt tcaacgattt
    16501 tgattcaacc attttaacat ttggaatata agaaatatag atagttgcaa aatctggttg
    16561 cccagtaatc ggacataatg atgtgaattc tggacagttg aattttacga aatagtcacg
    16621 accttgatgc ttattatcaa acgattctaa tacatcagga cgatagtcaa aattgtacgt
    16681 attgtcttga tttcctaata aagttatatc ttgtaattca tcttgttgac ggccatgtgc
    16741 cataaaaagc gctcctttaa atttattttt ttattacttt ggcgtctcgg cgtgcttttt
    16801 caaacatgta ataacttgca ccgataataa cgacgtaacc taatgttgca tagaaatctg
    16861 gagattctcc aaatagaata aatccaagta ttgctgtgaa aattatagat gcatatgtga
    16921 aaatagaaat atctttcgca gcagcaaaac tatatgccaa tgtaacgcca atttgacctg
    16981 cagctgcagc taaaccagca cctaatagat aaagcacttg cgtttgactc attggttcat
    17041 aggtatatgc agtgaaaggt attaaaacga tgacagaaaa taaggagaag taaaatacta
    17101 tagtatatgg tgcttctcta gtactaagtg cgcgtacaca cgtatatgcc gatgcagcaa
    17161 aaatacctga aaatagtcca gctagtgatg gaatcataga tgatgaaaat tcaggtttca
    17221 ctattaaaag catacctaaa atagcaatta tcattgctgt tatttgatat ttccttacct
    17281 tttcatgtaa gaaaacaata cttaataaaa tcgtccagaa aggattgagt ttcattagtg
    17341 aatcagcatc gctaagtacc atatggtcaa tggcgtaaat atttaataat acacctatga
    17401 gtccaagtgt tgatcgtgtt aataataatg gttggcttga gagtttacca aacattggtt
    17461 gatgatattt atatataaaa aataatggaa taaacattgc tactaagttt cgtgctaatg
    17521 atttttgaaa aacaggaaga tcacctgcaa gtctgaaaaa cactgacata aaactgaaac
    17581 caatagccga aattaaaatg gcaatgatac cttttacttt aggattcaat tttatcgcct
    17641 cttttatata aaattaacgt atttatatta gcataaaaca acatgttgtg cataaatagt
    17701 tgaaatttac tataaaaaga ctataataga ctttatcgaa caagtgttct gtgtttattt
    17761 gtcggaataa tagggcatta cacttttatg aatgtttgtg ttattacata aaacaaatat
    17821 caattcagta tcaagctaat aagctttttc ttgatttctg ttgatacaat tgagattgac
    17881 acagatttta aaaaatcaag tgatatctac tgaaaaaatt tttttaattt gttcaagttt
    17941 ttctaattaa gtattggtgc ctagttggaa cgttttacga acattcgatt agaaaatgac
    18001 actttaaatc atagtgtgtc ttatgtataa tgaaacacat aatatagtgt tggttaaacg
    18061 aaaaagacac aatatcttgt gttttgtatg caaatgcttt atttatgaag aaattacatt
    18121 taaaagtaat ttaacacaga aatttaatag ttattatcaa tgaatagtca tattttttag
    18181 aaaatgtact gagcaaatgg aaggtatcta atgatctaaa cactacatat agtgattttt
    18241 atacattcaa cccatataag ctactatttt ctcaaatata aatctatgca attggtttac
    18301 atttgagaaa ataagtagct tcattatagt taatacaatg ctgagataac catagtagag
    18361 acgttgttaa agcatttttt aattgtaatg actacttaat ttaaaagggt tgaagaaaga
    18421 aggtgatcca atgaaaataa tatatttttc atttactgga aatgtccgtc gttt
//
LOCUS       DAMZPP010000047        18417 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMZPP010000047 DAMZPP010000000
VERSION     DAMZPP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18417
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.047"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..1707)
                     /locus_tag="POH73_001213"
     CDS             complement(<1..1707)
                     /locus_tag="POH73_001213"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDI6887178.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DHASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHIGGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVKDIKVDVTTSQKIAREILIDRNGNAE
                     SIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKDIIDTIESICKRDPLAGKTVTGGIITINDSAWQISFT
                     INRQQQFKDQPKNEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVPTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSTFDLRVLMDAVYELNDHQDLREITKDSKMQK
                     LAL"
     gene            2058..2531
                     /locus_tag="POH73_001214"
     CDS             2058..2531
                     /locus_tag="POH73_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDI6887179.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYSKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
     gene            2793..4385
                     /locus_tag="POH73_001215"
     CDS             2793..4385
                     /locus_tag="POH73_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDI6887180.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISEDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
     gene            complement(4714..6240)
                     /gene="spa"
                     /locus_tag="POH73_001216"
     CDS             complement(4714..6240)
                     /gene="spa"
                     /locus_tag="POH73_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HDI6887181.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNKFNK
                     DQQSAFYEILNMPNLNEEQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDGNKPGK
                     EDGNKPGKEDGNKPGKEGGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIK
                     PGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL
                     "
     gene            complement(6661..7413)
                     /gene="sarS"
                     /locus_tag="POH73_001217"
     CDS             complement(6661..7413)
                     /gene="sarS"
                     /locus_tag="POH73_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HDI6887182.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMNDAQQDHAEQLLAQVNQLLADKNHLHLVFE"
     gene            complement(7782..8780)
                     /gene="sirC"
                     /locus_tag="POH73_001218"
     CDS             complement(7782..8780)
                     /gene="sirC"
                     /locus_tag="POH73_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HDI6887183.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMVGSSFIPLQRVLMYF
                     INPNDSIDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAIAMVILLVFQTKGHIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTFILGMVIVAVIPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLISSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(8777..9772)
                     /gene="sirB"
                     /locus_tag="POH73_001219"
     CDS             complement(8777..9772)
                     /gene="sirB"
                     /locus_tag="POH73_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HDI6887184.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALTGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYLMKKGRQRL"
     gene            complement(9788..10780)
                     /gene="sirA"
                     /locus_tag="POH73_001220"
     CDS             complement(9788..10780)
                     /gene="sirA"
                     /locus_tag="POH73_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HDI6887185.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDSKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            11011..11991
                     /gene="sbnA"
                     /locus_tag="POH73_001221"
     CDS             11011..11991
                     /gene="sbnA"
                     /locus_tag="POH73_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HDI6887186.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            11988..12998
                     /gene="sbnB"
                     /locus_tag="POH73_001222"
     CDS             11988..12998
                     /gene="sbnB"
                     /locus_tag="POH73_001222"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HDI6887187.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDTPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            13019..14773
                     /gene="sbnC"
                     /locus_tag="POH73_001223"
     CDS             13019..14773
                     /gene="sbnC"
                     /locus_tag="POH73_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HDI6887188.1"
                     /translation="MQNHTAVNTAQTIILRDLVDALLFEDIAGIVSNSEITKENGQTI
                     LIYKRETQQIKIPVYFSALNMFRYESSQPITIEGRASKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTQMIHGDTANIDELESLTAPIKEQATDMLHDQGL
                     SLDDYVLFPVHPWQYQHILPNVFAKEIREKLVVPLPLKFGDYLSASSMRSLINIAAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAERLLRQLIEKDAMLAKYVTVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KKDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            14766..16022
                     /gene="sbnD"
                     /locus_tag="POH73_001224"
     CDS             14766..16022
                     /gene="sbnD"
                     /locus_tag="POH73_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HDI6887189.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNVEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVIGQIIGSLSGAAITSYTTPATTFIVMGIVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            16012..17748
                     /gene="sbnE"
                     /locus_tag="POH73_001225"
     CDS             16012..17748
                     /gene="sbnE"
                     /locus_tag="POH73_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HDI6887190.1"
                     /translation="MQNKELIQHAAHAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGQFAYWSAMGHHMYHPEVWLIDGKSKKLTTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYAEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPIHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPTSKLSQVIEQSGLAPE
                     AWLECYLDRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPEVCYVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWKLV
                     AHRLMTWKKEYANNAVFIDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            17657..>18417
                     /gene="sbnF"
                     /locus_tag="POH73_001226"
     CDS             17657..>18417
                     /gene="sbnF"
                     /locus_tag="POH73_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HDI6887191.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMYQLVSS
                     LIYENIVVYKASYQDGVGYFTIEGNDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHP"
BASE COUNT         5870 a         2975 c         3431 g         6141 t
ORIGIN      
        1 taatgcgagt ttttgcattt tcgaatcttt agtaatctcg cgcaaatctt gatggtcatt
       61 cagttcgtat acggcatcca ttaagacgcg aagatcaaat gtactattga tgacctctgg
      121 aataccacga tctatattta gtaattgata aacagcttcc atggcagtac gaaccgaata
      181 ttctgttgta aatacagtat ctcgctctgt ttctgcaaag ttaccaataa atgctaaatt
      241 ttgtgattga tgcgggacga ctaaaggtct gtcgccgata gcacgcgtca tgaaataaga
      301 ggtaatatat ggcatataaa caggaatcgt attagatgca tgttttgcta agtcttcaat
      361 tttgtcagtt ggtacaccta agtgatatag ccattcttga catatttcat taccactaca
      421 ttctgtgatt ggctttttaa tataatcgcc gtttacatct gaatataagg cataaatcca
      481 cgtagatatt tcatttttag gttggtcttt aaactgttgc tgacgattga ttgtaaaact
      541 gatttgccat gcagaatcat tgattgtaat aattccgcca gtaaccgttt tgcctgcaag
      601 agggtcacgt ttacaaatgc tttctattgt atcgataata tctttattgt ttgttgtcga
      661 tgttgcagaa acaaaccaac tttttttagg aatattttgg caaaacttat caggattacc
      721 aaattcagga ctttgtcgtg ctaaattttt ccatagtgtc caactaccac ctaattcgtc
      781 agttggtggc gctggtgtat cattatcacc ataagtagag ctttctgtaa tactaccgtt
      841 tgtcacaaag acaagatcgt ttatagtcag tttaatagat tctgcattac cattacggtc
      901 aattaatatt tctcgggcaa ttttttgact tgtcgtaaca tctactttaa tatcttttac
      961 ttttacatcg tattcaaatt gaaccccatg cgattttaaa tattcaacca taggtaatac
     1021 taaggattca tattgattat atttagtgaa ttttaaagct gaaaagtctg cgagaccacc
     1081 aatatgatga acgaatcgca ttagatagcg acgcatttcc attgcagaat gccacggttc
     1141 aaatgcaaac atcgttttcc agtaaatcca aaagtttgaa ttaaagaaat cgtcggaaaa
     1201 tacatctgtt attttgacat catctaaatc ttcttcattc gttaagcata aatctaaaat
     1261 ttctttaatc gccgttttag tcaaagtgaa gtctccatct gtgactaaac gttgaccctg
     1321 tttctcaata acacgacagc gagaatagtt agggtcttct ttgtttaacc aatagaattc
     1381 atctaatact gacgcatgat caatttcaag tgaagggata gatctgaata aatcccacaa
     1441 acattcaaag tggttctcca tttcacgacc acctcggaca acatagcctt ttaaaggcat
     1501 attttcacca tcaagactac cgcccgcttt gggtaactct tctaaaatat gaatcttaga
     1561 accttccatt tgaccatccc ttattaaaaa acaagctgca gcaagtgaag ctagaccaga
     1621 tccgattaag taagcggatt tgttttctac attttcaggt tttttagggc gcgcaaatgc
     1681 ttcataattt ccataactgt aatacatatc caccaagtcc tttcgtatta gaatacactc
     1741 agactatacc cctttgtgaa ttgcaaatat tagtatttat attggtaaat ttgacgaaat
     1801 tagatcattt ttatattttt gatttttaaa attttatcac tcgaatgttt tttgattctg
     1861 aggtgcggga cttggaataa ttcgaatcat tatgtggcat gttaaacagc taaatataag
     1921 ctatgtacat ttaacaagcg gatactcctg acgcaattat agtatcatta catattgttt
     1981 aatcgtttgg ggtaatgtaa ttaaaaggta aaataggcag agtgttatac ataaaatgta
     2041 aagaaagagg cgatagaatg tctaaaatca ggtcttttac aatattaagt ctacttattt
     2101 acttagctat gatgtgctat acagtagtga cttattcaaa attaccaacc aaagtaccta
     2161 ttcactataa tttagcaggg gatgctgata actttgctga taaatgggtg ctgcttttga
     2221 ttaatagcgc atttatagtg atttggctta tatttttcat tgcaggtaga tactatgaac
     2281 gatttgccaa atggtcacat tataatcata caccacgtga aattcgagcg attaaattat
     2341 ttttaagtac gttaaattta gagattatga gctatatgtc tatcttcaca gtattagaaa
     2401 tttggcaaat acaacaccat catcaattca atttactatg gtttaatatg atatttatca
     2461 ttatcattgg tttgacgctt gtcatatttt gtttacttcc tacaattcat aaaatgagag
     2521 attctcaata aaaatataaa ctgtcatgat gcagttttga agcggattcc tgagattatt
     2581 ataaataata tctagtattt ataattaaaa tgatatatta tttattatag ttaaatattg
     2641 tgtatatttt gtgaactttt tgtaaaaatt cgattgcctg tcacatatag gagtgttaca
     2701 ttttaaatat gtgatcatcg caaaatataa gttgaaatag gttgtagatt aatcaggatg
     2761 ataaatattt tatataaaga gagggagtca ttatgacact acttactgta aatccattcg
     2821 ataatgttgg attatcagcc ttagttgcag cagtacctat tattttattt ttattatgct
     2881 taaccgtttt taaaatgaaa ggcatttatg cagcattgac aactttggtt gttacattga
     2941 ttgtggcttt atttgtattt gaattaccag cgcgtgtatc agcaggtgcg attacagaag
     3001 gcgttgttgc cggtattttc ccaataggat atatcgtttt aatggcagtt tggttatata
     3061 aagtttctat taaaacagga caattttcta ttattcaaga tagtattgca agtatttcag
     3121 aggaccaaag aatccaacta ttattaattg gattttgttt caacgcattt ttagaaggtg
     3181 cagcaggatt tggtgtgcca attgcgattt gtgcagtatt attaattcaa cttggatttg
     3241 aaccattaaa agcagcgatg ttatgtttaa ttgctaatgg tgcggcgggt gcctttggtg
     3301 caattggttt accagttagt attattgata cgtttaactt aagtggaggc gttacaacat
     3361 tagatgttgc gagatactca gcattaacac ttccaatttt aaactttatt attccatttg
     3421 ttttagtatt cattgtagat ggtatgaaag gtattaaaga aattttacct gtcattttaa
     3481 cagtgagtgg tacatatact ggattacaat tattattaac aatattccat ggtccagaac
     3541 tagcagacat tattccatca ctagcaacaa tggtggtgtt agcatttgtt tgtcgtaaat
     3601 ttaaaccgaa aaacattttc agattggaag cgtctgaaca taaaattcaa aaacgaacgc
     3661 ctaaagaaat tgtctttgct tggagtccgt tcgtcatttt aactgccttt gtattagtat
     3721 ggagtgcacc attcttcaaa aaattattcc aacctggagg tgcacttgaa agtttagtaa
     3781 taaaattgcc aattccaaat actgtgagtg atttatcgcc taaaggaatt gcgttgcgtc
     3841 tcgatttaat tggtgcaact gggacagcga ttttattaac agtaattatt acaattttaa
     3901 ttacgaagtt aaaatggaaa agtgcaggtg ctttattggt cgaagcaatt aaagaattat
     3961 ggttaccgat ccttacaatt tcagctatcc tagctattgc taaagttatg acatacggtg
     4021 gtttgactgt agcaattgga caaggtattg ctaaagcggg agcaattttc ccattattct
     4081 ctccagtatt aggttggatt ggtgtgttta tgactggttc agttgtaaat aacaatactt
     4141 tattcgcacc tattcaagcg acagtggcac aacaaatttc aacaagcggt tcattacttg
     4201 tggcggctaa cactgcaggt ggtgtagcag cgaaacttat ttcaccacaa tcaattgcca
     4261 ttgcgactgc agctgttaaa aaagttggtg aagaatctgc attattaaaa atgacgttaa
     4321 aatacagtat tatatttgtt gcctttattt gtgtttggac gtttatacta acgttaatat
     4381 tctaaatata aataatgttg tcacttggat tcaaatgaca ttttaaatct aattattcat
     4441 gaatcgaact agtacgaaat gcaatgagca tcttgtctag ttcgattttt taatgtctaa
     4501 aaatgtcgta tatgtaatca gagtagaaag tgttgaggcg tttcagaagt tgtttagaaa
     4561 agtaagtaaa ataaaaaatg cactgagcaa caaaagatgt tgctcgtgca tttagatgat
     4621 tcttatcatt tcaaataaga atgtgttaat caacgtatat aagttaaaat tggtttggat
     4681 aaaatgatat ctatcgttgt gtattgtttg tttttatagt tcgcgacgac gtccagctaa
     4741 taacgctgca cctaacgcta atgataatcc accaaataca gttgtaccga tgaatggatt
     4801 ttcttcacca gtttctggta atgcttgagc tttgttagca tctgcatggt ttgctggttg
     4861 cttcttatca acaacaagtt cttgaccagg tttgatcatg tttttatcag ctaatttgtt
     4921 atctgcagca attttgtcag cagtagtgcc gtttgctttt gcaatgtcat ttactgtatc
     4981 accaggttta acgacatgta ctccgttgcc gccttcttta ccaggcttgt tgccatcttc
     5041 tttaccaggc ttgttgccat cttctttacc aggcttgttg ccgtcttctt taccaggttt
     5101 gttgccatct tctttgccag gttttttgtt gtcttcttta ccaggtttgt tgccgtcttc
     5161 tttgccaggt tttttgttgt cttctttacc aggtttgttg ccgtcttctt taccaggctt
     5221 gttgttgtct tctttgccag gcttgttgtt gtcttcctct tttggtgctt gagcatcgtt
     5281 tagcttttta gcttctgcta aaatttcttt gctcactgaa ggatcgtctt taaggctttg
     5341 gatgaagccg ttacgttgtt cttcagttaa gttaggtaaa tgtaaaattt catagaaagc
     5401 attttgttgt tctttgttga atttgttgtc agcttttggt gcttgtgcat catttagctt
     5461 tttagcttct gctaaaaggt tagcgctttg gcttgggtca tcttttaagc tttggatgaa
     5521 accattgcgt tgttcttcat ttaagttagg taaatgtaag atttcataga aagcattttg
     5581 ttgttctttg ttgaatttgt tatcagcttt cggtgcttga gattcattta actttttagc
     5641 ttctgctaaa aggttagcac tttgacttgg gtcatctttt aagctttgga tgaaaccatt
     5701 gcgttgttct tcgttcaagt taggcatgtt caagatttca tagaaagcat tttgttgttc
     5761 tttgttgaaa ttgttgtcag ctttcggtgc ttgagattcg tttaattttt tagcttcacc
     5821 taaaacgtta gtgctttggc ttggatcgtc tttaagactt tgaatgaaac cattgcgttg
     5881 ctcttcgttt aagttaggca tgttcaagat ttcatagaag gcgctttgtt gatctttgtt
     5941 gaacttattt tgttgcgcat cagcttttgg agcttgagag tcattaagtt tttgagcttc
     6001 acctaaaacg ttagcacttt ggcttggatc atctttaagg ctttggataa aaccattacg
     6061 ttgatcagcg tttaagttag gcatatttaa cacttgataa aaagcatttt gttgagcttc
     6121 atcgtgttgc gcagcatttg cagcaggtgt tacgccacca gatataagta atgtacctaa
     6181 agttacagat gcaataccta cacctagttt acgaattgaa taaatgtttt tctttttcaa
     6241 attaataccc cctgtatgta tttgtaaagt catcataata taacgaatta tgtattgcaa
     6301 tactaaaatc tatatttata attaaattta aaggtaagtt ttacaactta taaaataaaa
     6361 attttgtcca aagatttatt caggaagtaa aaggcttaaa accgcaaaat cacgctattc
     6421 cgattaataa aaacatataa aatatataga tattttttat aatgaaagcg atttattaca
     6481 tatgagttaa aaaatatttt taagaataaa atggaatttt gaatgtgctg aggtttaatt
     6541 tcggaaatgg ttttaagaga tatagaaaat agaaagttgt tcataatgtg ctgaggtttt
     6601 attaaaaaat agaaaacaca agtgcacttt agaatacagc acacttgcgt aatagagata
     6661 ttattcaaaa acaagatgta aatgattttt atctgccaat aattgattca cttgagctaa
     6721 taattgttca gcatggtctt gctgcgcgtc attcatatga attaaaattt ttctttcatc
     6781 ttcagttgag cgttctttta ttagatagcc ttgctttttt aaattattga gagctctaac
     6841 agtttgaggg tatttatggt ggattgtttc aattaaatct ttaagaagaa cgatgttttt
     6901 attttgagaa gtgataatag ctagaattgt gaattctaca aaacttaatg ttagatgctt
     6961 tttgataata ttcttgaaat acattgtata catcatcaag tttagaaatt ctttactatc
     7021 ttttggtatc atctgtgatt cactttgatc tgcaagatta aattgtttaa tgatttgatc
     7081 aaacaatgta acacgttctg caattttctc tcgttgttct tcagatattg aaatgtaagt
     7141 attacgctca tcaattttac ttcgaacttt actaatatat gaatgtttca caagtacttt
     7201 tatatgctgt actaaatccg attgtttata acataaatct gaaacaatct tcttaaatgg
     7261 aagtgtgttt tcttgctgat gaaataaata agtcagtaat ataaattctt ttatagtcat
     7321 atcgacttca ggcttgactt ttttcttaaa acgaaacata tatgcttcaa tgattataaa
     7381 atctctaatt ttgtcatggt tattatattt cattgttcta tctccttgta tatgcacttt
     7441 attttaatat aacacaatat aataagaaaa ataatccata atttacaatt aaaaataata
     7501 tgatattttt attaaaattt gtgtttttga ataaatgaca ttatcaaaca ctatatttaa
     7561 ataagaaata gttatcgata tttgaaaaat cgaagaatat tgattgatat tatagaatat
     7621 gagtcggtgt tatttgtata taaagtatta atatgtaatt aagaaagtcg acgcttgtta
     7681 acattaacaa caactatatg tataaaaaaa cctcgcaact gttagttagc tgaatcattt
     7741 tgctaatgtt gttgtgaggt cattttgttt taataatatc tttataactt tttcacggtt
     7801 aataataagt atatgaagaa tggtgcacca aacgcagcaa tgaatacacc tgccggcact
     7861 tctttaggca agaatagggt gcgtccaatt aagtctgcga taacaattga tatggcacca
     7921 atcattgctg acattagtaa ctttttagca taacttccgc gaacgattgt tttcgcgatg
     7981 tgtggtgcaa ttaaaccgac aaacccaata tttcctacta aactgattgc catagatacg
     8041 agtattgtag aagagattaa ttgaatcagc ttcatacgtt gtacatgtaa acctaagcca
     8101 atcgctacag ggtcatcaag tatagatatt ttcattttag gtataacaag aaataacaac
     8161 ggaataacag ctacgattac catacccaaa atgaatgtat ctttaaacgt tgcaccgtaa
     8221 agacttccga ctaaccaagt ataagctttg gctgcagata gttgctttgt tgtaataagt
     8281 agcccttgta caagtgcaat aaataatgtt tgcattgaaa taccgatgat tatgagtgtt
     8341 gtcggtcgaa tgtgaccttt cgtttgaaac actaatagaa tcaccattgc aattgcgcca
     8401 cctaatacag caaatagagg aagtaaatgt atcgttaaat ggctgaaaaa tgcaataaag
     8461 acaacagcac ttaagctagc accacctgtg attccgataa tatctggtga ggcaattgga
     8521 tttttcaaga cattttgtaa cattaaaccg ctcatcccta gtgctgcacc agctaatatc
     8581 gcaagtgtaa tgcgaggtaa gcgtaatact tctaaagtga attgatctat actgtcattt
     8641 ggatttataa agtacatcag tacgcgttgt aatggtataa agcttgaacc aaccatcata
     8701 cttaccactg aaacgatggc taaaaagatt aacgcgaaga tgagatggta attgtccttt
     8761 ttattaatct tttcggtcat aagcgttgac gtcctttctt cataagatag attaagacaa
     8821 tagcgccaat gacagcggta acgacaccga taggcaactc tagtggctta ataataatac
     8881 gagcaacaat gtctgagata atcattagga ttgcgccggt taatgcagta aaaggaatta
     8941 aatacttata gtttggtggt aataatcgtt tactaatatt cggtacaata agtcctacaa
     9001 agacgattga tccagctaca gcaaccgaga tgcctgctag catactaatg agcattataa
     9061 tcatccattt gattaatttt atgttttgtc cgagtccagt ggcaatgtcg tcacttgtca
     9121 tcaagatgtt gatgtgtgca gccatgctaa atgcaattaa aataagtatc aatacaagcg
     9181 gaataatcca tgggatatcc caaatgttac gtaatgaaac ggagccactc aaccagaata
     9241 ataggccttg taagtctgtt tcattcataa tgagaatgcc ttgagtaaaa gctgtaaata
     9301 acatcgcaat cgcggcaccc gctaaaatga cacggtgagg tgaaaatagg gtttgtctaa
     9361 acatccctag tgcaacgact aagactgtaa caacaatagc acccaaaaac gcgatgacaa
     9421 caatcatttt aaaagattga atttggataa atgtaatact aaagatgaca aaaaatactg
     9481 cgcctgcatt gacaccgaaa agccccggcg atgctattgg gtttcgtgta agtgcttgca
     9541 tcaacaaacc tgagactgct agtgcggcgc ctgtcaatag cgcaatgatt gttctcgacg
     9601 cccgtgcacc agtgacaaca tcatgtaaat cgttttcact atcaaagttg aataacgcct
     9661 gtatcaccgt acctggtgac acaagcgtat ttccaatcat taaacttaag atagctacta
     9721 ttgcaagaca taaaccagca ataacgattt ggtattttgg tttaagtagc atcgtaaaac
     9781 tccttaatta ttttgattgt ttttcaatat ttaacttttc atataaatcg tcaataagtt
     9841 ttaatgatga tttatatccg ccagctaagt tccaagtgat ttcatctaaa tcatcagata
     9901 cttggttgtt tttaactgcg tctaaatttt tccactcttt acttgaagtc cattcgcttt
     9961 cagtcttttt aactaatgca gcatctttcg catttggatc tgattttact acaaaaatat
    10021 gatcagcgtt cattaatgga atgctttctt tagatgtaag ttggataata tctttaccat
    10081 tatcaacttg tttttgtaag tctttattac gtttgaatcc taaatcattt aagatttcac
    10141 cagcatatcc accagcataa attcttgtat gatcagcacg gaagttaaca actgaagctt
    10201 tcaatggcca tgcatcttta tactttgctt ttgcatcttt ttggaatgca gctactttat
    10261 catcgtactt tttaagtaaa tcttcagctt ctttttcttt ccctaaagct ttccccatta
    10321 acttagttgt atctttgaat ttgaaaactg tatcagtaga aactgttggt gcgattttag
    10381 ataattgatc gtaaactttt tcatttctaa cttttgacgc gacaattaag tccggtttta
    10441 atttagagat ttcttctaag ttaggtgcag gttcttgacc tacaatctta gtatctttta
    10501 aatcattttt tatgtattcg aatttcggtt tttgtgtcca tgattctaca gcacctacag
    10561 gtttaacacc taaagataca gcgacgtcag tggcaccttg atatagtgta acaacgcgct
    10621 ttggtttccc tttaatttca gttgtaccca ttgcatgttt aattgaagtt gtttccttat
    10681 ctttgctatc agatgattgc ttatttgaat tcccactaca tcctgctaaa acaagtagga
    10741 aagcaagcgt aacaacaagc attttaatta ctttattcat tgactaatta gcctccttcg
    10801 tgatgtatga caatgagaat cattatcacg gtttagtatg aattaaattt tttcctaagt
    10861 caataaaata tttatgattt acatgcaact tataattatt tgacatataa atgcataaaa
    10921 aatataatcc taattacttg atagtgagaa tcattatcaa ttaggtaaca cacaatatta
    10981 tagaatttta aatttgagga ggaagcgctt ttgattgaaa aaagtcaagc atgtcacgat
    11041 tcattgttag attctgtagg gcaaacacct atggttcaac ttcatcaact atttccgaaa
    11101 catgaagtgt ttgcaaagtt agagtatatg aatcctggag gcagcatgaa agatcgacct
    11161 gccaagtaca tcattgaaca tggtattaaa catggtttaa tcactgagaa tacacattta
    11221 attgaaagta cttctggtaa tttaggcatt gcgttggcaa tgatagctaa aatcaaggga
    11281 ttaaaactca cgtgtgttgt tgatcctaaa atatcaccaa caaatttgaa aattattaaa
    11341 agttatggtg ccaatgtaga aatggttgaa gaacctgatg cacatggggg ttatttaatg
    11401 actcgtattg caaaggtgca agaactgtta gccactattg acgatgcata ttggattaat
    11461 caatatgcga atgagttaaa ttggcaatcc cattatcatg gtgcaggcac agagattgtt
    11521 gaaacaatta agcaacctat agattatttt gtcgcgccag tcagcacgac aggtagcatt
    11581 atgggtatga gtagaaaaat taaagaagtg catccaaacg cacaaattgt tgctgttgat
    11641 gcgaaagggt cagtcatttt tggtgacaaa cctattaata gagaattacc tggtatcggt
    11701 gctagtcgtg tacccgaaat attgaataga tcagaaatta atcaagtgat ccatgtagat
    11761 gattatcaat ctgctttggg ctgtcgaaaa ctgattgatt atgaaggtat atttgctgga
    11821 ggttcaacag gttcgattat tgcagcgatt gagcagttga taacgtcaat tgaagaaggt
    11881 gcaacaattg tcacgatttt accagatcga ggcgatcgtt acttagattt agtttattca
    11941 gatacatggt tagaaaaaat gaaatcaaga caaggagtta aatcagaatg aatagagaga
    12001 tgttgtattt aaatagatca gatattgaac aagcgggagg aaatcattca caagtttatg
    12061 ttgacgcatt aacagaagca ttaacagcgc atgcgcacaa tgattttgta caaccgctta
    12121 agccgtattt aagacaggat cctgaaaatg gacacatcgc agatcgaatt attgcaatgc
    12181 caagtcatat cggtggtgaa cacgcaattt caggtattaa gtggataggt agtaagcacg
    12241 acaatccatc gaaacgtaat atggagcgtg caagtggtgt cattattttg aatgatccag
    12301 aaacgaatta tccaattgca gttatggaag caagtttgat tagtagtatg cgtactgcag
    12361 cagtttcagt gattgcagct aagcatttgg ctaaaaaagg atttaaagac ttaacaatca
    12421 ttggttgcgg gctaatcgga gacaagcaat tacaaagtat gttagagcaa ttcgatcata
    12481 ttgaacgcgt ttttgtttac gatcaattct ctgaagcgtg tgcacgcttt gttgatagat
    12541 ggcaacaaca gcgtccggaa attaatttta ttgcgacaga aaatgctaaa gaagcagtat
    12601 caaatggtga agtagtcatt acatgtaccg taacggatca accatatatt gaatatgatt
    12661 ggttacaaaa gggtgcattt attagcaaca tttctatcat ggatgtgcat aaagaagtct
    12721 ttattaaagc tgacaaagtc gtagtagatg actggtcaca atgtaatcga gaaaagaaaa
    12781 ctattaacca attggtgtta gaaggtaaat tcagcaaaga agcacttcat gctgaactag
    12841 ggcaacttgt gacaggtgac acaccaggac gtgaagacga tgatgaaatc atattactta
    12901 atccgatggg tatggctatc gaagatattt caagtgctta ctttatttat caacaggcac
    12961 aacaacaaaa tattgggaca acattgaacc tatattaaga atgcgaggtg tctgaacatt
    13021 gcagaatcat acagcagtca atacagcaca aacgataata ttaagggatt tagttgatgc
    13081 attattattt gaagatatag ccggtattgt atcgaatagt gagattacta aagaaaatgg
    13141 acaaaccatt ttgatataca aacgtgaaac acaacaaata aagatacctg tttattttag
    13201 tgctttaaat atgtttcgtt atgaaagttc acaaccaatt acgatagagg gaagggcgtc
    13261 taagcaacct ttaacggcag ctgaattttg gcaaacaatc gctaatatga attgtgattt
    13321 aagtcatgaa tgggaagtgg ctcgcgttga agaaggactg actactgctg ccacacagct
    13381 tgctaaacaa ttatcagaat tagatttagc gtcgcatcct tttgtgatgt ccgaacagtt
    13441 tgcaagttta aaagatcgtc catttcatcc attagctaaa gaaaaaagag gattaagaga
    13501 agcggattat caagtgtatc aagctgaatt aaatcaatca tttcctttaa tggttgcagc
    13561 agttaaaaag acacaaatga ttcatggcga tactgcaaat attgatgaat tagaatcatt
    13621 gacagcacct ataaaagaac aagcgacaga tatgttacat gatcaagggt tatcactaga
    13681 tgactatgta ctatttcctg tacatccttg gcaatatcaa catattttgc cgaacgtctt
    13741 tgcgaaagag attagggaaa aattggttgt accattacca ttaaaatttg gagattatct
    13801 atcggcttca agtatgcgtt cattaattaa tatcgcagcg ccgtataacc atgttaaagt
    13861 gccatttgca atgcagtcat taggcgcatt aaggctaacg cctacgcgtt acatgaaaaa
    13921 cggagaacaa gccgaacgat tattacgtca acttatagaa aaagatgcca tgttagctaa
    13981 gtatgtcaca gtttgtgatg aaacagcttg gtggtcatat atgggtcaag ataatgatat
    14041 tttcaaagat caattaggtc atttaactgt tcagctaaga aaatatcccg aagtgctagc
    14101 caaaaatgat acgcaacagc tagtgtcaat ggcagcactc gcggcaaatg atcgcacctt
    14161 atatcaaatg atttgtggaa aagataatat ttctaaaaaa gatgtcatga cgttatttga
    14221 agatatcgca caagtctttt taaaggtaac actatcattt atgcaatacg gcgcattacc
    14281 agagttgcat ggtcaaaata tattgttgtc atttgaagat ggacgtgtac aaaaatgcgt
    14341 gttacgtgat catgatactg tcagaattta taaaccatgg ctaacagcac atcagctttc
    14401 attgccgaag tatgtcgtca gagaagatac acctaatacg ctaattaatg aagatttgga
    14461 aacattcttt gcttattttc aaacattagc tgtatcggta aatctatatg ccattattga
    14521 tgcaattcaa gatttatttg gtgtaagtga gcatgaactt atgtcgttgt taaaacaaat
    14581 tttaaaaaat gaagtggcaa ctatttcctg ggttacaact gatcagctag ctgtcagaca
    14641 tattttattt gataaacaga cgtggccatt taaacaaatt ttattaccat tgctatatca
    14701 acgtgatagt ggtggaggta gtatgccttc aggtttaact accgtaccaa atccaatggt
    14761 gacatatgat taatcagtct atatggcgca gtaactttcg cattttatgg ctcagtcagt
    14821 ttatagcgat tgctggactg acagtacttg tgccattatt gccaatttat atggcatcac
    14881 tacaaaatct atcagtcgta gaaatacagt tgtggagtgg tatagcgatt gctgctccag
    14941 ctgtaacgac gatgatagct tcgccgatat gggggaagct aggtgataag atcagtcgaa
    15001 aatggatggt gctacgagcg ttacttggtt tggcggtatg tttattttta atggcattgt
    15061 gtactacacc attacagttt gtacttgtga ggttattgca gggactattt ggtggtgttg
    15121 ttgatgcatc aagtgcgttt gcgagtgcag aggcgccagc tgaagatcgt ggaaaggtat
    15181 taggaagact gcaaagttca gtcagcgcag ggtctcttgt gggaccatta attggcggtg
    15241 ttacagcttc gatattaggt tttagtgcgt tactgatgag tattgccgtt attactttta
    15301 ttgtctgtat tttcggtgca ttaaaattga ttgaaacgac acatatgcca aaatcacaaa
    15361 caccaaatat taataaaggt attcgccgtt catttcaatg tctattatgc acacaacaaa
    15421 catgtcgatt tattatcgtt ggcgttttag caaactttgc tatgtatggc atgctaactg
    15481 cattatcacc acttgcttca tcagtgaatc atacagcgat agatgaccgt agtgtgattg
    15541 gatttttaca gtccgcattt tggacggctt cgatattaag cgcgccttta tggggtcgct
    15601 ttaatgataa atcatatgtt aaatcagtat atatatttgc cacgattgca tgtggttgta
    15661 gtgcgatact gcaaggttta gcgacgaatg tagaattttt aatggctgca agaatacttc
    15721 aaggattaac atatagtgca ttgattcaaa gtgtcatgtt tgtcgtcgtg aatgcgtgtc
    15781 atcaacaact taaaggcaca tttgttggta cgacaaatag tatgttagtt attggtcaaa
    15841 ttattggtag tcttagtggc gctgccatta caagttatac tacaccagct actacgttta
    15901 tcgttatggg catagtattt gctgtaagta gtttattttt aatttgttca accatcacta
    15961 atcaaatcaa cgatcacaca ttaatgaaat tatgggagtt gaaacaaaaa agtgcaaaat
    16021 aaagaattaa tacaacatgc agcgcatgcg gctatcgaac gtattttaaa tgaatatttt
    16081 agagaagaaa atttatatca agtaccacct caaaatcatc aatggtctat acaattatct
    16141 gagctcgaaa cgttaacagg tcaattcgct tattggtctg cgatggggca tcatatgtat
    16201 catccagaag tatggcttat tgatggtaaa agtaagaaat taacaacata taaagaagcc
    16261 attgcgcgta ttttgcaaca tatggctcaa agtgcagata atcaaacggc agtgcaacaa
    16321 catatggcgc aaattatgtc tgacatcgat aatagcattc atcgcacggc gcgttatttg
    16381 caaagtaaca caatagacta cgcagaggat cgctatatcg tttcagaaca atctttatat
    16441 ttaggtcatc catttcatcc gactcctaag agtgcaagtg ggttttcaga agcagattta
    16501 gagaaatatg cacccgaatg tcatacatca ttccaattgc attatttagc tgtgcatcaa
    16561 gatgttctgc tcacgcgcta tgtagaaggt aaagaagatc aggttgagaa agtgttgtat
    16621 caattagcag atatagacat atcagagata cccaaagatt ttattttatt accaatacat
    16681 ccttatcaaa tcaatgtgtt gcgacagcat ccacagtata tgcaatatag tgaacaaggt
    16741 ttgataaaag accttggcgt ttccggtgat tcagtgtacc cgacgtcttc ggttagaact
    16801 gtattttcaa aagcattaaa catttatttg aaattaccga tacacgttaa aatcactaat
    16861 tttatacgta cgaatgacct tgaacagatt gaacggacaa ttgatgccgc gcaagttatc
    16921 gcatcagtca aagatgaggt tgaaacaccc cattttaaat tgatgtttga agaaggatat
    16981 cgtgcattgt taccgaatcc attagggcaa acagttgaac ctgaaatgga tttattaaca
    17041 aatagtgcca tgattgttcg tgaagggata ccgaattacc atgctgataa agatattcat
    17101 gtattggcgt cattatttga aacgatgcct gattcaccga cctctaagtt atcacaagtg
    17161 attgagcaaa gtggtttagc accagaagca tggcttgaat gttatttgga tcgtacatta
    17221 ttgccgatat taaagctgtt tagtaacaca ggcattagtc tagaagcaca tgtacaaaat
    17281 acattaattg aattaaaaga tggcatacca gaagtgtgct atgtcagaga tttagaaggt
    17341 atttgtctat ctagaacgat tgctactgaa aaacagcttg tgccaaatgt tgttgcagca
    17401 tctagccctg ttgtatatgc acatgatgaa gcgtggcatc gtcttaaata ttacgttgta
    17461 gtaaatcact taggacattt agtatcaact attggtaaag cgactagaaa tgaagttgtg
    17521 ttatggaaac ttgtagcgca tcgtcttatg acttggaaaa aagaatatgc gaataacgca
    17581 gtatttattg actgtgtaga agatttatat caaacgccga ccattgcggc taaagcgaat
    17641 ttgatgagta aattgaatga ttgtggtgca aaccctattt atacacatat accaaatcca
    17701 atttgtcata acaaggaggt atcgtattgt gaatcaaaca attcttaatc gtgtaaagac
    17761 tagagtgatg taccaattgg tatcatcgct tatttatgag aatattgttg tgtataaagc
    17821 gtcatatcaa gatggtgtcg gttattttac aatagaagga aatgattcag agtatcgttt
    17881 tactgctgaa aagacacata gctttgatcg tatacgtatc acatcaccaa ttgagcgtgt
    17941 cgtaggagat gaggcagata caacaacaga ctatacacaa ttactgagag aggttgtatt
    18001 tacatttcct aaaaatgatg aaaagctaga acaatttatt gtcgagttat tacagacaga
    18061 actaaaagat acgcaaagta tgcagtatcg agaatcaaac ccaccagcaa cacctgagac
    18121 atttaacgac tatgaatttt atgcgatgga agggcatcag tatcatccaa gttacaaatc
    18181 acgtttagga tttacgttga gtgataattt gaaatttggt cctgactttg taccaaacgt
    18241 taaactgcag tggttagcta tcgacaaaga taaagtagaa acgacggtat caagaaatgt
    18301 tgtagttaac gaaatgttac gtcaacaagt tggcgataag acttatgaac attttgtaca
    18361 gcaaattgaa gcgtctggca aacatgtaaa tgatgttgag atgatacctg tacaccc
//
LOCUS       DAMZPP010000048        18376 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMZPP010000048 DAMZPP010000000
VERSION     DAMZPP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18376
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.048"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..267
                     /locus_tag="POH73_001227"
     CDS             <1..267
                     /locus_tag="POH73_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6887192.1"
                     /translation="SEISGGQKQRVAIAKALYTNPSIILADEPTAALDTENAIEVIKI
                     LRDQAKKRKKACIIVTHDERLKAYCDRSYHMKDGVLNLENETVE"
     gene            475..1506
                     /locus_tag="POH73_001228"
     CDS             475..1506
                     /locus_tag="POH73_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI6887193.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPMTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            1849..2967
                     /locus_tag="POH73_001229"
     CDS             1849..2967
                     /locus_tag="POH73_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDI6887194.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKDLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            2942..3865
                     /locus_tag="POH73_001230"
     CDS             2942..3865
                     /locus_tag="POH73_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDI6887195.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     VTTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVVAMK
                     RIGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAVLGAKIAVAVNKLL"
     gene            3876..5096
                     /locus_tag="POH73_001231"
     CDS             3876..5096
                     /locus_tag="POH73_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDI6887196.1"
                     /translation="MNILFAITGIAFALFVAFLFSYDRKNIDFKKTLIMIFIQVLIVI
                     FMMNTTIGLTILTALGSFFEGLINVSKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPKL
                     SRAKLYTIATSGMSAVSMTMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     TDVEIDNLTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(5201..6733)
                     /locus_tag="POH73_001232"
     CDS             complement(5201..6733)
                     /locus_tag="POH73_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDI6887197.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLLIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYAHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(6773..7654)
                     /locus_tag="POH73_001233"
     CDS             complement(6773..7654)
                     /locus_tag="POH73_001233"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDI6887198.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            7812..8672
                     /locus_tag="POH73_001234"
     CDS             7812..8672
                     /locus_tag="POH73_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDI6887199.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQIPTPDNKSELITDKV
                     YEIVTGYMKQYHLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIDGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(8949..9749)
                     /locus_tag="POH73_001235"
     CDS             complement(8949..9749)
                     /locus_tag="POH73_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI6887200.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            9889..10557
                     /locus_tag="POH73_001236"
     CDS             9889..10557
                     /locus_tag="POH73_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDI6887201.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     IMED"
     gene            complement(10683..11996)
                     /locus_tag="POH73_001237"
     CDS             complement(10683..11996)
                     /locus_tag="POH73_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDI6887202.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KFGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAIDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIII
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPITNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQAVLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
     gene            12413..14488
                     /gene="lip2"
                     /locus_tag="POH73_001238"
     CDS             12413..14488
                     /gene="lip2"
                     /locus_tag="POH73_001238"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HDI6887203.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVTSHEAQASEKIPTT
                     NAAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDMLQSSKHQST
                     QNSKIIRTQNDNQVKQDSERQGSKQSHQNNATNKTERQNDQIQNTHHAERNGSQSTTS
                     QSNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKEQDATTDKHPNQQDT
                     HQPAHQIIDAKQDDNVHQSNQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQA
                     PKTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNK
                     YKVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGK
                     TYKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTG
                     GHNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGF
                     KQLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLNGSAKLNNMTSMNPNITYTTYTGVS
                     SHTGPLGYENPDLGTFFLMDTTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVT
                     NDEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATE
                     IKGTQLKAS"
     gene            complement(14730..15557)
                     /locus_tag="POH73_001239"
     CDS             complement(14730..15557)
                     /locus_tag="POH73_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6887204.1"
                     /translation="MTEIKDKVITKDAFELPYTIIKAKHQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIDDIFTSFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSGINTEPFKMANSYYAKIAQSVNETVLAQLTS
                     PTPIIQDQIAQRFLIYGYARGTGEWINMINIADYTDSKYNISPNELKTLPPVFIAHCN
                     GDYDVPVEESEHIHNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITTA"
     gene            complement(15615..16829)
                     /locus_tag="POH73_001240"
     CDS             complement(15615..16829)
                     /locus_tag="POH73_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDI6887205.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNASTKEGYITKADL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRETPEETRGSDLGYTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGEHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLSEQRPHDIDLEFSMADLENLAIPRAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDTSSKDNFS"
     gene            16925..17023
                     /locus_tag="POH73_001241"
     CDS             16925..17023
                     /locus_tag="POH73_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887206.1"
                     /translation="MESIFKIKIELMNVICRSENMNMKKKNTSEKI"
     gene            17137..18138
                     /locus_tag="POH73_001242"
     CDS             17137..18138
                     /locus_tag="POH73_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDI6887207.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSETSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKYFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEHFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEQVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            18172..>18376
                     /locus_tag="POH73_001243"
     CDS             18172..>18376
                     /locus_tag="POH73_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDI6887208.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGT"
BASE COUNT         6272 a         2898 c         3574 g         5632 t
ORIGIN      
        1 tcagaaattt caggtggtca gaaacaacgt gtggcgatag ccaaagcgtt atatacgaat
       61 ccgtcgatta ttttagcgga tgaacctacc gcggcgttag atactgaaaa tgcgattgaa
      121 gtcattaaaa ttctacgtga tcaagccaaa aaaagaaaga aagcatgtat tattgttaca
      181 catgatgaac gacttaaagc atattgtgat cgttcatatc atatgaaaga tggcgtcctt
      241 aatcttgaaa atgaaacagt agaatagttt tattaagccg gtacatcatg tgccggtatt
      301 tttatgttta tgtattattt gaataaactt tcacattcaa ttaataataa ttattatcga
      361 aaattagaaa tattccgtga aatataatat tttttgtagt aaaatggcct ctaagtattc
      421 aatatttaaa tatggggatt gaatataaaa ttatcgtaat gggggtcaat ggttatggat
      481 ttattaatag gtactttatt tttatttttg gtcttagtga tttttacatt atttacatat
      541 aaagcaccta atggtatgcg cgccatggga gcattagcaa atgcagcaat cgcaacattt
      601 ttagtggaag catttaataa atatgttggt ggtgaagtgt tcggtattaa atttttagaa
      661 gagctaggag acgctgcggg aggtctaggt ggtgtcgctg ccgctggatt aacagcatta
      721 gctatcggtg tgtcaccagt atatgcatta gttatagcag ccgcgtgcgg tggtatggat
      781 ttattaccag gtttctttgc gggttatatg attggatatg tgatgaaata tacagagaaa
      841 tatgtgccgg atggtgtcga cttaattgga tcgattgtca tcttagcgcc attagctcgt
      901 cttattgcag tattattaac gccagtagtg aatagtacat tgattcgaat tggtgatatt
      961 atccaaagta gtacgaatac gaatccaatt atcatgggta tcattttagg tggtattatt
     1021 acggttgtcg gcacagcgcc attgagttca atggcattga cagcattatt aggtttaacg
     1081 ggtgtaccta tggctattgg tgccatggca gcatttagtt cggcatttat gaatgggacg
     1141 ctattccatc gcttaaaatt aggtgatcgt aagtctacga ttgcagtaag tattgaacct
     1201 ttatcacaag cagatattgt atcagccaat ccaattccaa tctatattac aaatttcttt
     1261 ggtggtgcga ttgctggttt aattattgct atgtcaggtt taattaacga tgcgacaggt
     1321 acagctacac cgattgcagg atttttagta atgtttggat ttaatcatcc aatgactatt
     1381 gtaatttatg gtgtagtaat ggcgattgta ggtgcgcttg caggttatct tggttcaatc
     1441 gtatttaaaa aatatccaat tgttactaag caagacatga ttaatcgagg tgcagttgac
     1501 gcatagcatc atcatattga atagtaaaaa caaataaaac atagtaacgt gattcagtcg
     1561 atgaaaaaat cgataatgag tcacgttttt ttatagaaaa atataagaca taaaaatgtc
     1621 ataatttata gtcgacaaat atcacgccac ataaacactt atcattttcg taggtgcctt
     1681 ttactttatg gaatgaactt actttttacg aaattatgcg tattttataa acaaatatca
     1741 ttgatataac ggtaaatgta agcgtttaca acagaaataa ctgcatgcta cgatattttt
     1801 gtaaattcac tgattcaagt attttaagtc aatatgagga gggatgttat gagcgattct
     1861 gagaaagaaa ttttaaaaag aattaaagat aatccgttta tttcacaacg tgaacttgct
     1921 gaggcaattg gattatctag acccagcgta gcaaacatta tttcaggatt aatacaaaag
     1981 gaatatgtta tgggaaaggc atatgtttta aatgaagatt atcctattgt ttgtattggc
     2041 gcagcgaatg tagatcgtaa gttttatgtg cataaagatt tagttgcaga aacatcaaat
     2101 cctgtaacgt caacacgctc tattggtggc gtagcaagaa atattgctga gaacttaggt
     2161 aggcttggcg aaacggtcgc ttttttatct gctagtggac aagatagtga atgggaaatg
     2221 attaaacgat tgtccacacc atttatgaat ttggatcatg ttcaacaatt tgaaaatgcg
     2281 agtacaggtt catatacagc tttaattagt aaagaaggcg acatgacata tggcttagca
     2341 gatatggaag tgtttgacta cattacgcct gaatttttaa ttaagcgttc acacttattg
     2401 aaaaaggcta agtgcattat tgtcgatttg aatttaggca aagaggcatt aaacttctta
     2461 tgtgcctata ccacgaaaca tcaaatcaaa ttagttatca ccacggtttc ttccccaaaa
     2521 atgaaaaata tgcctgattc attacatgct attgattgga ttatcacgaa taaagatgaa
     2581 acagaaacat acttaaattt aaaaatagaa tctactgatg atttaaaaat agctgctaaa
     2641 cgctggaatg atttaggtgt taaaaatgtt attgtgacaa atggcgtgaa agaactcatt
     2701 tatcgaagtg gtgaggaaga aatcatcaag tcagttatgc catcaaatag tgtgaaagat
     2761 gttacaggtg caggtgattc attctgtgct gcagtagtgt atagctggtt aaatgggatg
     2821 tctactgaag atatattaat tgctggtatg gttaatgcaa agaaaacgat agaaacgaaa
     2881 tatacagtta ggcaaaacct agatcaacag caactttatc acgatatgga ggattataaa
     2941 aatggcaaat ttacaaaagt atattgagta ttctcgagaa gttcagcaag cacgggagaa
     3001 caatcaaccg attgtagcat tagaatcaac aattatttcg catggtatgc cgtacccaca
     3061 aaatgttgaa atggtaacaa cagtagagca aattatcagg aataatggtg ccattccagc
     3121 aaccatagcc attatagatg gcaaaattaa aattggttta gaaagcgaag atttagaaat
     3181 actggcaact agtaaagacg ttgctaaagt atctagaagg gatttagcag aagttgttgc
     3241 gatgaagcgt attggtgcta ctactgtagc gacgacgatg atatgtgctg caatggctgg
     3301 tattcaattt tttgttactg gaggtattgg gggtgtccat aaaggtgcag aacatacgat
     3361 ggacatttca gcagacttag aagaattgtc taaaacaaat gtcactgtta tctgtgcagg
     3421 tgccaaatca attttagact tacctaagac gatggaatat ttagaaacaa aaggcgttcc
     3481 agttattgga tatcaaacga atgaattgcc agcattcttc actcgcgaaa gcggtgttaa
     3541 gttaacaagt tcggttgaaa cgccagaacg acttgctgac attcatttaa caaaacagca
     3601 gttaaatctt gaaggtggca ttgttgttgc taatccaatt ccatatgagc atgccttatc
     3661 aaaagcatat attgaggcaa tcataaatga agctgttgtt gaagccgaaa atcaaggtat
     3721 taaaggtaag gacgccacac cgttcttgtt agggaaaatt gtagaaaaaa cgaatggtaa
     3781 aagtttagca gcaaatataa aacttgttga aaacaatgcg gtgttgggtg ctaaaattgc
     3841 tgtcgctgtt aataaattat tgtaggtgat gatacatgaa tattttattc gctatcacag
     3901 ggatagcatt tgcactattt gttgcgtttt tattcagtta tgatcgtaaa aacatagact
     3961 tcaaaaagac gttaataatg atatttattc aagtgttgat tgtgatattt atgatgaaca
     4021 caacgattgg tttaacaatc ttaactgcat taggttcttt ttttgaaggg ttaataaatg
     4081 ttagtaaagc aggtataaac tttgtttttg gagatataca aaataaaaat ggctttacat
     4141 tctttttaaa tgtgttgctg ccattagtgt tcatttctgt attaataggc atttttaatt
     4201 atattaaggt attaccattt attatcaaat atgtaggtat tgctattaat aaaataacga
     4261 gaatggggcg tttagaaagt tactttgcta tttcgacagc gatgtttgga cagccagaag
     4321 tgtatttaac tataaaagat attataccaa agttgtctag agcaaaatta tatacaattg
     4381 cgacatctgg tatgagtgct gttagtatga caatgttggg ttcatatatg cagatgattg
     4441 agcctaagtt cgtagttaca gcagtaatgt taaatatttt tagtgcgctt atcatcgcca
     4501 gtgtaatcaa tccctataaa tctgatgata ctgatgttga aattgataac ttaacgaaat
     4561 ccacagaaac taaaacattg aatggaaaaa cagggaaacc taagaaagtt gcctttttcc
     4621 aaatgattgg tgatagtgcg atggatgggt ttaaaatcgc tgttgtagta gccgtaatgt
     4681 tgttagcatt tatttcatta atggaagcaa ttaatatcat gtttggtagt gttggtttga
     4741 actttaaaca gcttattggc tatgtgtttg caccaatcgc attcttaatg gggattccat
     4801 ggagcgaagc tgttccagct ggctctttaa tggcgaccaa attaattaca aatgagtttg
     4861 tagcaatgct tgattttaaa aatgtcctgg gtgatgtatc tgctcgaaca caaggtatca
     4921 tttcagttta cttagtaagc ttcgctaatt ttggtacggt tggtatcatc gtaggttcaa
     4981 ttaaaggcat tagtgataaa caaggagaaa aagttgcatc ctttgcaatg aggctgttac
     5041 ttggttcaac tctagcttca atcatttcag gatcaatcat tggcctagta ttataaatta
     5101 atcgaagtat ctaaattaaa ttcatggcaa agctaaaccc catcaccaag gtggcgcaac
     5161 agcgcatcat aacttagtga cggggtttta tcataacaat ctactttttc gtagccgttt
     5221 ttgaaatgta tgttgatggt ttatcttttt caaaaattgt taatcccgtt atatcttttt
     5281 tatgttttga agggacaatg aagctaagta tataagcaaa gacaaaagca actgtaaatg
     5341 aaatggtaga tacatagaaa ggtgagttac ctttgccaac accattatag acataagcaa
     5401 agatgatacc caatattaat ccacaaataa caccgaatgt attcgtacgt ttagtgaaaa
     5461 taccaactgc aaatacacca gccaatggaa cgccgaataa tccagttaca aacaagaata
     5521 aatcccataa gtcatttgaa ttagaagcaa ttaagtatag tgacattcca aaaccgaaaa
     5581 tacctgcaat gatgataatg aaacgtgcaa agttaacttc gtgtcgttcg ctaccttttc
     5641 cgaagaagcg ttgcttaatg tcgattgaaa tacaagcaga tatagaattt aaactagatg
     5701 aaatggtaga ctgtgcagct gcgaaaatgg ctgcaataag taatcctgct acaaatggtg
     5761 gcatctcagt caaaatgaaa tatggcacta cagatgatgt attgaagcct tttggtaaaa
     5821 cagcttcatg tgcataaaat gaatacaaca ttgtacccat accataaaat aagggtgctg
     5881 aaattaaagc taggatacca tttgtccata acgatttatt tgtttctttt aaactatcag
     5941 aagcttgata acgctgcacg acgtcttgac tcgccgtgta ttgatacaaa ttgttgaaaa
     6001 tatttcctag gaaaataatt ggaatggcag ctgctgcagt attcagtttc caattgtctg
     6061 cactaattaa ttttttgtgc tcaatcgcat ctgcaaagac agtgccaaaa ccgcctttaa
     6121 tgttcataac accaagaatg atgataacta aagcgccgcc taatagaatg acgccttgaa
     6181 tgaaatcact ccaaaccaca ccttcgaaac cacctaaaaa tgtatataaa atacatagta
     6241 aaccaacgag tgatgcaacg atataagggt tcatgtctga tacagatgta atcgctaatg
     6301 ttggtaagta gataacgatt gctacacgcc ctagatgata aacgacaaat aataatgagc
     6361 caataacacg aatgctaggt ccgaaacgtg cttctagata ttcatatgca gatgttacct
     6421 ttaacttttt aaagaatgga acatagaaat aaatgagtaa tggaataatt gctacgatag
     6481 cgatgttacc tgcaatatat gaccaatctg ttaaaaatgc tttctctggt gtagacataa
     6541 acgtaattgc acttaatgta gtggcataaa ttgaaaaccc aactacccaa gatggcaagc
     6601 gaccacttgc agtaaagaaa ctattcgtac tttggctcgc gcgcttagta aaataaacgc
     6661 caatgagtaa catagctagt agatatatga taacggcaac ccagtttagt gtgccaaacc
     6721 caacttcttt catgggcaac atccccttta caatgtattg attctttgat gtctataaat
     6781 cgtattttgc aatgagttga tctaatgttt gtcgatgtgc ttcgttaaaa ggtttgaatg
     6841 gacgtttcgg taatcctgca tcaatgccac ggtgacgtag tatttctttc aatgttggat
     6901 aaatccccat tgataacact gtttcgatga tgtcgtttga atcatgttgt agttggtaag
     6961 cttcttgaat ttgaccttgt cttgctaagt cgaagatttt tctagcgcgg cgaccattaa
     7021 cgttatatgt agaaccaatt gcaccatcta cgccagaaat cgtagcttga actaacattt
     7081 catcaaagcc agataagatt aatttgtctg ggaatgcttt tctaatacgt tcgagtagga
     7141 agaagtttgg cgctgtatat ttaacaccaa caattttttc gtgattaaaa agctcgctga
     7201 attgttcaat agaaatattc acacctgtta aatctggtat tgcataaata atcatattgt
     7261 tctgtgttgc ttcgataata tcgaaatagt aatctctaat ttcttcaaaa gtaaatggat
     7321 agtagaatgg tgttacggca gaaagtgcat cataaccgag ttctgtggca tattttccga
     7381 gttcaatggc ttcatttaaa tctaacgaac ctacttgagc aatcaatttc actttatccc
     7441 caactgcctc tttggcaact ttgaaaactt gcttcttctg ttctgtattt aataaaaagt
     7501 tttcgcctga gctaccattt acataaagac cgtctaattc ttcagtttca atggcatttt
     7561 gagcaatttg tttaagtcct tgttcattta cttgaccatt ttcatcaaaa ggaacgagta
     7621 acgctgcata taaacctttt aaatctttgt tcattatgaa gtccctccaa aaatcatttg
     7681 ataatatagt ttacagctat aattgtaaac gctatcataa aatgtaacaa tatctttttg
     7741 aaaattgtag tcatatttat gtataattaa tgaaaatttt tttcaaaatc aatagaaatg
     7801 gagtgagtaa ggtgtattac atcgcaatcg atattggagg cactcaaatt aaatcggcag
     7861 ttattgataa gcaattgaat atgtttgact atcaacaaat accaacgccg gacaacaaaa
     7921 gtgagcttat tactgacaaa gtatatgaga ttgtaacagg atatatgaag caatatcatt
     7981 tgatccaacc tgtcataggt atttcatcag caggcgttgt tgatgaacaa aaaggcgaaa
     8041 ttgtatacgc agggccaacc attccgaatt ataaaggtac taattttaag cgattattaa
     8101 aatcactgtc tccttatgtc aaagtaaaaa atgatgtaaa cgctgcatta ctaggagaat
     8161 tgaaattaca tcaatatcaa gcagaacgga tcttttgtat gacgcttggt acaggcattg
     8221 gtggtgcgta caaaaataat caaggccata ttgataatgg tgaacttcat aaggcaaatg
     8281 aagttgggta tttattgtat cgtccaactg aaaatacaac gtttgagcaa cgtgctgcaa
     8341 cgagtgcatt gaaaaagcgc atgattgacg gaggatttac gagaagcaca catgtgccag
     8401 tattgtttga agcagctgaa gaaggtgatg atattgcaaa acaaatattg aatgagtggg
     8461 cagaagatgt agcagaaggg attgcccaaa tacaagtcat gtatgatcca gggcttatat
     8521 taattggtgg cggtatatct gaacaaggag acaatctcat taaatatatc gagccgaaag
     8581 ttgcacacta tttaccaaaa gactatgttt atgcaccaat acaaacgact aagagtaaaa
     8641 atgatgcagc attatatggc tgtttgcaat gatggttgaa agaaggagtc attctaaaat
     8701 agaatttgaa accgttacga gagatgagag ctgttgttag ttccacagat tatgttatat
     8761 ccatgaccaa tctaaactat atcaaccaac agtgtgacac gggcatatta aattgaagaa
     8821 gctgagatat taaaatttta gaaaatgtaa aaaatatttg gtattgaaat taaaaaagca
     8881 cctagcaact cgttgggaca atcacgatga ttgtctacag ttgcaggtgg atttgaatat
     8941 actactagtt atttgttgtc taggataata gatttagtat gttgataagt ttgactcaga
     9001 ttcgtatttt ctaataaatg atagctcacg atatcgatta aaaagagtgt cgcaatttgt
     9061 gtgttgataa attgatggtc ggtattacgc gattgatccg ttgttaaaag tactaaatct
     9121 gcacaatctg taagtttact accttcgaaa tttgtgatgg caacgacata tgcaccatga
     9181 gatttggcga cttccgctgc agaaattaat tccgaagtat taccactatt tgacatagca
     9241 ataaacatat ccgaatgaga tagtagggat gccgatattt tcattaaatg tgaatcggta
     9301 gtaacattac cttttagccc catacgaatc atacgataat aaaattcagt cgctgataaa
     9361 ccagagctac ctagtccagc aaagagtata tgtcgacttg attgaagttt gtcgataaag
     9421 gtttggataa tgtcgttatc aataaattca ccagtttgtt gaatgatttg ttgatgatat
     9481 ttatgaattc tttgaataat tgggctattt tcaataactg tctctgtcat ttcttgttga
     9541 atattaaatt ttaaatcttg gaaattctca taatccagct tatgactaaa gcgtgtcatc
     9601 gttgctggtg atgtaccaat cgcatgggct aaggagttaa tcgttgaaaa ggcatcgcta
     9661 tagccatttt gtcttatata attgacgatg cgtttatcag tttttgtaaa taaatgttga
     9721 taacgttgaa cacgattctc aaatttcatt gtgtcacccc ttcatcttaa tgattactat
     9781 tatatatgaa aaatattttc aagatagtaa aaagcattga taaaaattat cttaatgata
     9841 tattgtaaat gactttacgt gaaaaaacga cttatggagt gaggaataat gttaccacat
     9901 ggattaatag tatcttgtca ggcactacca gatgaaccat tgcattcatc ttttattatg
     9961 tcgaaaatgg cattagctgc gtatgaaggt ggtgctgttg gtattcgtgc aaatactaag
    10021 gaagacattt tagcaattaa agaaacggta gatttaccag ttattggcat tgtgaaacgt
    10081 gactatgatc actcagatgt tttcattaca gcaacgtcaa aagaagttga tgaactgata
    10141 gaaagccaat gtgaagtcat tgcattggat gcaacgttac agcaacgtcc gaaagaaacg
    10201 ttagacgaat tagtatcata tattagaaca catgcaccga acgttgaaat catggctgat
    10261 atcgcgaccg ttgaagaagc taaaaatgcg gcacgacttg gctttgatta tattggcacg
    10321 acgttacatg gctatactag ttatacgcaa ggacaattac tttatcaaaa tgacttccaa
    10381 tttctaaaag atgtactaca aagtgttgat gcaaaagtta ttgcggaagg taatgtcatt
    10441 acaccggata tgtataaacg cgtgatggac ttaggcgttc attgttcagt cgttggtggt
    10501 gcgataacac gaccaaaaga aattacgaaa cgttttgttc aaattatgga agattaaatg
    10561 ataacgataa aaaacgagat gaccatcatt aattaaaggt acctaattat cttaggtggc
    10621 tgaatgaatg taatgggttc atctcgtttt gtttgtttat gatagtgatt ttattttcaa
    10681 ctttatccaa aaataagtaa agcgacgggg atggtgatta atagcgacaa tgccacgcgt
    10741 aaaaaccaaa tgatgatgag tttccagaca ggtattttaa tttcagttgc tagtatacat
    10801 ggcactaatg ctgagaaaaa gataatggct gatacgctta ctacaccgac gacaaattta
    10861 gtactcattg cagctttagt tactaacaaa gatggtagaa acatttctac aatagaaatc
    10921 gctgaagctt ttgcaagtac agcctgatca gcaattggga aaatataaat aaatggatag
    10981 aatatgtagc caagccaatc aatgaatggt gtatagttcg ctacaatcag ccctaaaaaa
    11041 ccaatcgata atatagaagg taaaatacca acagtcattt ctaaaccgtc tttcaaattg
    11101 tcccaaacgt tcttcacgag ggatggtgtt aatgcatttt gtttcatcgc ctctgcatat
    11161 gcagttttca gtctgcttcc ttcaatagca acttcttgtt ctccttcttg tccgttataa
    11221 tattctgttg attcattggt gattggcggt agccatgcag taattgcagt cacgacaaat
    11281 gtgatgacta aagttatcca aaagtataaa ttccaatgag gcattaatcc taaagtttta
    11341 gcaatgataa tcataaaagt tgctgaaact gttgaaaagc cagtcgcaat aatcgtggct
    11401 tctcgtttgt tgtacatccc ttgcttatag acacgattag taatcaataa tcctaaggaa
    11461 taactgccga caaacgaagc cactgcatcg atagcggatt ttcctggtgt tttaaaaata
    11521 ggtctcataa taggctccat ataaacaccg acaaattcta ataagccata acctactaat
    11581 aaagaaagcg caattgcacc tactggaatt aagatactta atggcatcat taatttttca
    11641 aacaaaaacg gaccatagtt agctttaaat agtattgatg gaccgaattt aaatacatac
    11701 attataccga tcattgcacc tgcaacttta aataatgtaa tgaccaagtt tgtgattgaa
    11761 gtcataaaag tacgtctcac tattggtaac gctgtaccaa ttaaaatcat aatcagtgca
    11821 acatagggca taagtggacc tatgattgag cgaatggcta gatgaacatg atcgacgaaa
    11881 atagtgttgt taccattaat cgtaaaagga ataaagaaac atagtatgcc cactaaacta
    11941 tagacaaaaa aacgccatgc acttggttgt tgtgcattag aatgatattg attcattaaa
    12001 gcaacccctt tgtttaaatg aatacacaaa actgtatgat gcatcttccc cttaatgaga
    12061 tgaatcattg ttttaattta gaaaaatctg aaaacttact ataattgtat agtttggatt
    12121 attttcagac caatacaaat taactaatta tatatagatt gaaactatat agcttaataa
    12181 aatatttatc ttaaatgttg ttgtgttgat tcaacgtcac aattaaagta tttataaatt
    12241 atttgaggat acagatattt gtaaataatt agtatcgatt taatatcgta ttattaaatt
    12301 tttattaatt ttgtagtctt aatcaaaaaa taatatatgt catgttatat tgaaagtgca
    12361 gttgtttttc attctcaaga ggggatcaaa aaaatacttt tgaggtgatt atatgttaag
    12421 aggacaagaa gaaagaaagt atagtattag aaagtattca ataggcgtgg tgtcagtgtt
    12481 agcggctaca atgtttgttg tgacatcaca tgaagcacaa gcctcggaaa aaataccaac
    12541 gactaatgca gcggcacaaa aagaaacact aaatcaaccg ggagaacaag ggaatgcgat
    12601 aacgtcacat caaatgcagt caggaaagca attagacgat atgcataaag agaatggtaa
    12661 aagtggaaca gtgacagaag gtaaagatat gcttcaatca tcgaagcatc aatcaacaca
    12721 aaatagtaaa ataatcagaa cgcaaaatga taatcaagta aagcaagatt ctgaacgaca
    12781 aggttctaaa cagtcacatc aaaataatgc gactaacaaa actgaacgcc aaaatgatca
    12841 gattcaaaat acccatcatg ctgaacgtaa tggatcacaa tcgacaacgt cacaatcgaa
    12901 tgatgttgat aaatcacaac catccattcc ggcacaaaag gtaataccaa atcatgataa
    12961 agcagcacca acttcaacaa cacccccatc taatgataaa actgcaccta aatcaacaaa
    13021 agaacaagat gcaaccacgg acaaacatcc aaatcaacaa gatacacatc aacccgcgca
    13081 tcaaatcata gatgcaaagc aagatgataa tgttcatcaa agtaatcaga aaccacaagt
    13141 tggcgattta agtaaacata tcgatggtca aaattccccg gagaaaccga cagataaaaa
    13201 tactgataat aaacaactaa tcaaagatgc gcttcaagcg cctaaaacac gttcgactac
    13261 aaatgcagca gcagatgcta aaaaggttcg accacttaaa gcgaatcaag tacaaccact
    13321 taacaaatat ccagttgttt ttgtacatgg atttttagga ttagtaggcg ataatgcacc
    13381 tgctttatac ccaaattatt ggggtgggaa taaatataaa gttatcgaag aattgagaaa
    13441 gcaaggctat aatgtacatc aagcaagtgt tagtgcattt ggtagcaact atgatcgtgc
    13501 tgtagaactt tattattata tcaaaggtgg tcgcgtagat tatggcgcag cacatgcagc
    13561 taaatacgga catgaacgct atggtaagac atataaagga atcatgccta attgggaacc
    13621 tggtaaaaag gtacatcttg tagggcatag tatgggtggt caaacaattc gtttaatgga
    13681 agagttttta agaaatggta acaaagaaga aattgcctat cataaagcgc atggtggaga
    13741 aatatcacca ttattcactg gtggtcataa caatatggtt gcatcaatca caacattagc
    13801 aacaccacat aatggttcac aagcagctga taagtttgga aatacagaag ctgttagaaa
    13861 aatcatgttc gctttaaatc gatttatggg taacaagtat tcgaatatcg atttaggatt
    13921 aacgcaatgg ggctttaaac aattaccaaa tgagagttac attgactata taaaacgcgt
    13981 tagtaaaagc aaaatttgga catcagacga taatgctgcg tatgatttaa cgttaaatgg
    14041 ctctgcaaaa ttgaacaaca tgacaagtat gaatccaaat attacgtaca cgacttatac
    14101 tggtgtatca tctcatactg gtccattagg ttatgaaaat cctgatttag gtacattttt
    14161 cttaatggat acaacgagta gaattattgg tcatgatgca agagaagaat ggcgtaaaaa
    14221 tgatggtgtc gtaccagtaa tttcgtcatt acatccgtcc aatcaaccat ttgttaatgt
    14281 tacgaatgat gaacctgcca cacgaagagg tatctggcaa gttaaaccaa tcatacaagg
    14341 atgggatcat gtcgatttta tcggtgtaga tttcctggat ttcaagcgta aaggtgcaga
    14401 actcgccaac ttctatacag gtattataaa tgacttgttg cgtgttgaag cgactgaaat
    14461 taaaggaaca caattgaaag caagttaaat tcatattcgt aatttaatat gctatgtatt
    14521 ttgtgctact aacatggcac ggaagatata agtagcatca cagtgttgaa ttttaaaata
    14581 gtaaagtgaa ataaagcgcc tgtctcatta gcgaaaacta aagggacagg cgtatctgtt
    14641 tatgagctta ataaattgta tgaataatat ggttgattga ataactgttt atcatgatga
    14701 taaattgggt ttttaaaaat aatggtatat tacgccgttg ttatagcgtt taagaaatca
    14761 acaactttac gataaatagt gattgcttcg tcattaggtc tacgatcaaa atcatgctcg
    14821 tttttattca cgcgttcaaa tgttgagtgt ggcacatgat tgtggatgtg ttcgctttct
    14881 tcaacaggga catcataatc gccattacaa tgcgcaatga acaccggggg aagtgtttta
    14941 agttcatttg gtgagatgtt gtattttgaa tcagtataat cagcaatgtt aatcatattg
    15001 atccactcac ctgtaccacg tgcataaccg tagattaaaa aacgttgggc gatttgatct
    15061 tgaataatcg gtgttggtga agtaagttga gcaagtactg tttcattcac actttgagct
    15121 attttagcgt aatagctatt tgccatttta aacggttcag tattgatgcc actataacca
    15181 taaaaatcaa taacaccatc aatatctctg tctcgtgcaa ttaatagact taaatatgca
    15241 cctgatgatc tgccaaaggt aaaaataggg caattagaat attgtgattg aatcgcatcg
    15301 aatgatgtga agatgtcatc gatgatgcaa tcaaggctta cttctggtaa taaacgataa
    15361 cttagttgaa ttaaatcgta atgttccgta aggatatcga tatactgtgg cgataaatca
    15421 ttggcctttc caaacattaa accgccacca tgaatgtaga caatggcacc ttttgttggt
    15481 tgatgcttcg ctttaataat tgtgtagggt aattcaaatg catctttagt aattacttta
    15541 tctttaattt cagtcacgac tatacagact ccttattttt gaaattgatg tcattataac
    15601 attgtcttaa atttctatga aaaattatct tttgatgatg tgtcatgata attctgttct
    15661 aattgacgta aagcgtcacg ggtatgcttc tttagacctt ccccataatc catcatttta
    15721 acaatatctt taaaagcggc acgcggaatg gctaaatttt ctaaatctgc catagaaaat
    15781 tcaaggtcaa tatcatgtgg tcgctgttca gaaagtttat gcacaaagtc aggttctgtg
    15841 acaaaaggcg aagacatgcc gaccatatct gcatgttgta aagcatctaa agcagactct
    15901 ggagaattaa tcccgccact tgcaattaaa gggatgcgac cagctaaatg ttcatagaca
    15961 atttggttaa ctggccgacc gaaatgttca cctggtgtac gagacgtatt ttgataaata
    16021 tgtcgacccc agctagcgat tgctaaatat tggatgtttg aaacgtccat gacccaatcg
    16081 attaattgat tgaactcgtc aatggtatat cctaaatcac tacctctggt ttcttctggc
    16141 gtttctcgaa atcctaaaat aaaattgtca ggtgcttctt tatcaatcac ttcttgtacc
    16201 gcacgcataa cttctaaaca taatcttgca cgatttttta atgagtcggc accgtaatgg
    16261 tctgtacgtt tattcgaaaa agttgagaaa aatgtttgaa tcagcaaacg ttgtgcaatc
    16321 gaaatttcca caccatcaaa acctgcttta atcgcgcgta atgtagcatc gcgatactgc
    16381 tgaatgatgc tattgatttt ctcatgagac atggcgataa catcgtgttc aatcggtgaa
    16441 tgcaacgtca taggacttgg tccatacacc tttccaaaat ttaaaattgc ttgatttgaa
    16501 aaacgaccag catgcgctag ctgaataata gcgaggctac catgttgttt catcgtagat
    16561 gccatgttag ttaatccagg gatacaagca tcatgatcaa tattaaagcc gtattcaaac
    16621 aattgaccat aaggttcaat gtaagcagcg ccggtgactt gcataccagc tgaattagag
    16681 cgacgtgcag cataagccaa gtctgctttt gtaatatagc cttcttttgt tgatgcgttt
    16741 acggtcattg gtgataatac aaagcgattc gaaattttga tgccattagg taagtggatt
    16801 gattgtaaaa gtggtttgta tcggtacata ctatgattcc ttttctattc aatattgttt
    16861 tcaaagtacc atggaaagaa tgaataatca atgatgaaca gtcttgatag aatagaattg
    16921 gtacatggaa agcattttta aaattaaaat tgaactaatg aatgtcattt gtaggtctga
    16981 aaatatgaat atgaaaaaga aaaatacaag cgaaaagata taaaagttaa ttgaaaaacg
    17041 ttatcatata cgtgggtata tgaagaggga atggtattaa gaacgttaaa atgttatgtc
    17101 ggtttgatat gacaggataa gtttggagat gacggattgg ttaaattaag cgtattagac
    17161 tatgccttaa tagatgaagg taaggatgca caaaaggcgt tgcaggattc agtgacactt
    17221 gcaaaattag cagatcgact tggctttaag cgaatttggt ttacagaaca tcataatgta
    17281 ccagcgtttg cgtgtagtag tccagaactt ttgatgatgc atacattggc gcagacaaat
    17341 cacatacgag ttggttctgg tggtgtgatg ctgccgcact atcgacctta taaaattgct
    17401 gagcatttta gaatgatggc agcgttatat ccaaatcgta ttgatttagg tattggtaat
    17461 aatccgggta ctactatggt aaagcaagct ttagatggaa taaatcctac atatgatagt
    17521 tacgatgaat cgatttcgtt attacgtgat tatcttacaa taaaggataa gccaagtgcg
    17581 catacgttag gtgtccaacc acacattgat cattttccag aaatgtggtt attaagtagt
    17641 agcgaaacat ctgccaaaat agctgccgaa ctaggtatag ggctttctgt tggaacattt
    17701 ttgctaccag atataaatgc gatacatgca gcgaaggata acattgatat ttacaaaaaa
    17761 tatttccaag catcaacgat taaaatggac gcaaaggtga tggcatctgt atttgtcatt
    17821 gtagctgata acgaagcgga agtagcagca ttacaacatg ccttagatgt ttggttatta
    17881 ggtaaattac aatttgcaga atttgaacat tttccatcgg tagacactgc gcaaaagtat
    17941 aagctcaatg atcgagacaa agagatgatt caagcacatc aagcacgtat cattgcaggt
    18001 acacaagaac aggttaaagc acaattagat gatttcattg ctacgtttga agttgatgag
    18061 gtgttagtag caccgcttat tccaggcatt gaacagcgtt gtaaaacatt aaaattactc
    18121 gcggaaatct atttgtagca ttttaaatag aagagaaagg atgaagataa gatgaaaaag
    18181 ttagccaatt atttatgggt agaaaaagta ggagatttgt atgtgtttag tatgacacct
    18241 gaattgcaag atgatattgg gacagtaggt tatgttgaat tcgtgagtcc agatgaagtt
    18301 aaagtggatg atgaaattgt gagtatcgaa gcatcgaaaa cggtcattga tgtgcaaacg
    18361 ccattgtcag gaacga
//
LOCUS       DAMZPP010000049        18154 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMZPP010000049 DAMZPP010000000
VERSION     DAMZPP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18154
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.049"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..393
                     /locus_tag="POH73_001244"
     CDS             <1..393
                     /locus_tag="POH73_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDI6887209.1"
                     /translation="TKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQIEDATVNVT
                     IKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILHFVSGDGGK
                     LKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            390..2051
                     /locus_tag="POH73_001245"
     CDS             390..2051
                     /locus_tag="POH73_001245"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDI6887210.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVAAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            2315..2950
                     /locus_tag="POH73_001246"
     CDS             2315..2950
                     /locus_tag="POH73_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HDI6887211.1"
                     /translation="MLGTDELYKLLYRHMGPQNWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIRPSGFYKSKSLTIKTLLTWLARYHFNYQ
                     EINERYKGELRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYNKLGYENTKS
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNCDFLEPYFKK"
     gene            3269..4156
                     /locus_tag="POH73_001247"
     CDS             3269..4156
                     /locus_tag="POH73_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6887212.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSDDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHEKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            4306..5256
                     /locus_tag="POH73_001248"
     CDS             4306..5256
                     /locus_tag="POH73_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6887213.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQIIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVIGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            5311..6030
                     /locus_tag="POH73_001249"
     CDS             5311..6030
                     /locus_tag="POH73_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6887214.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKIDNKGENYGSIY"
     gene            6014..6814
                     /locus_tag="POH73_001250"
     CDS             6014..6814
                     /locus_tag="POH73_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6887215.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSEDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLMLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTASFINKHHDEKHV"
     gene            6950..7456
                     /locus_tag="POH73_001251"
     CDS             6950..7456
                     /locus_tag="POH73_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887216.1"
                     /translation="MKKLLTASIIACSVVMGVGIANTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            7618..8334
                     /locus_tag="POH73_001252"
     CDS             7618..8334
                     /locus_tag="POH73_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887217.1"
                     /translation="MRINDKILLENIEDYFNHKGLSPHLIDDIKEKVITDIKNSEKKD
                     QDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFIINFILISYLYDKQYVQFQAITGMS
                     LFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMVMGTIIAIISLLLIILQAFNITWGVIP
                     ITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDAMIHNPQSAQTFSLIIW
                     ILLVVLVIYFTIRLSSRTRL"
     gene            8503..9291
                     /locus_tag="POH73_001253"
     CDS             8503..9291
                     /locus_tag="POH73_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6887218.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVITYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFLSYYLNFLKSVS"
     gene            complement(9541..9915)
                     /locus_tag="POH73_001254"
     CDS             complement(9541..9915)
                     /locus_tag="POH73_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDI6887219.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
     gene            complement(10863..11792)
                     /locus_tag="POH73_001255"
     CDS             complement(10863..11792)
                     /locus_tag="POH73_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI6887220.1"
                     /translation="MLLFYIIGVLVGMLIPIQTSVNSRLSLYTKSPFYTSFISFSVGT
                     ICLIVLNIFINPDVFSLHFFSNQTFNYTWFVGGLLGVGYLTGNLLLLPKLGATLTVIA
                     TVAGQIIMGVIIDTFGLFGATIHDFNLIKAIGVLLLIIGIVIMNQFNKNNLLLTDQKY
                     LLFWLLLGFIFGFFPPIQTTINSLLASHTHSPAFASLVSFTIGSITLLILTAIFNRSL
                     KLKTSHLKFGKLKPIYFTGGILGMAFVTSNIILMPHLGAALTTLIGMFGQILMGILID
                     HFALFGSPKIAMTSRKTIGLLCILTGIIFLRLF"
     gene            complement(11985..12209)
                     /locus_tag="POH73_001256"
     CDS             complement(11985..12209)
                     /locus_tag="POH73_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDI6887221.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNIVTEQVARDSMNALVDLN
                     ILKSNSGAITKVHSAQIIDKTTTVLFEAKK"
     gene            complement(12226..12429)
                     /locus_tag="POH73_001257"
     CDS             complement(12226..12429)
                     /locus_tag="POH73_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887222.1"
                     /translation="MSKTNHITIVLSFTKLDTNGKQTEFKRRFANINPEVSNDQIKTF
                     SKLIERLTGETYNNIEVIKSVSI"
     gene            12584..13144
                     /locus_tag="POH73_001258"
     CDS             12584..13144
                     /locus_tag="POH73_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6887223.1"
                     /translation="MNKVEVIKCNDDINKMYDALKARSDRDYLFFKLAIHSGMKITEL
                     LTITVEDTKRLIEKGTLSELCKAHYHSLIKIRLPETLSNELLHYIENKSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQAAIKAGIENVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            13163..15565
                     /gene="mnhA2"
                     /locus_tag="POH73_001259"
     CDS             13163..15565
                     /gene="mnhA2"
                     /locus_tag="POH73_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HDI6887224.1"
                     /translation="MSLVYLLIAILVIMAMILLTSKRRAMAKYAGYIALTAPVIASIY
                     FLLQVPSVIKQHYLSVSIPWMTSLDINVDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYLYLTLFMFSMLGIVLADNTILMYVFWELTSVSSFLLISYWYNNGDSQ
                     FGAMQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHALFIPMIIMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYIYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFAALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMMIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIIIVCMGVIASIFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVPALRGV
                     SGGNHQIDPLVPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     GYEMVYRQFEKFTTKRFKRVMQDRLNQYIIMTLGIFMVIIGYGYIRIGLPKVHQLHVS
                     EFGPLEVILAIVTVIIGLSLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSKVNKKREIIKISVSLMMALIVVSLIFIAQQADGLAS
                     ISNFYLRADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            15552..15977
                     /gene="mnhB2"
                     /locus_tag="POH73_001260"
     CDS             15552..15977
                     /gene="mnhB2"
                     /locus_tag="POH73_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HDI6887225.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIMPTFFGKPFLSQYETTLT
                     LPILGHIHVTTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            15974..16318
                     /gene="mnhC2"
                     /locus_tag="POH73_001261"
     CDS             15974..16318
                     /gene="mnhC2"
                     /locus_tag="POH73_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HDI6887226.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSNKSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            16308..17804
                     /gene="mnhD2"
                     /locus_tag="POH73_001262"
     CDS             16308..17804
                     /gene="mnhD2"
                     /locus_tag="POH73_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HDI6887227.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWTAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNGLIHPLLV
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPFFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVHFKKIPQYRKG
                     ILSILVVVVIAIGIAAPVLLKITNDATELNTNDQLYQKLVNPHLKGEE"
     gene            17805..>18154
                     /gene="mnhE2"
                     /locus_tag="POH73_001263"
     CDS             17805..>18154
                     /gene="mnhE2"
                     /locus_tag="POH73_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HDI6887228.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     AITFLTILIIITPGS"
BASE COUNT         6381 a         2424 c         3182 g         6167 t
ORIGIN      
        1 acaaagcaag tgttgaaaca gcacaacgaa aaagaaaaat ttgaatttac tactgaagga
       61 acttggcaac aaaggcaatc taactttatt cggtatgtag aacaaattga ggatgcaaca
      121 gttaatgtta caataaaagt ggatgatgat agcgttaagt tgattcgtaa aggcgacatt
      181 aatatgaatt tgcattttgt tgaaggacaa acgacaacaa ctttttacga tatatcggct
      241 ggacgaattc cactagaagt taaaacatta cgcattttac atttcgtaag tggagacggt
      301 ggcaagttaa agattcatta tgaattatat caagataatg aaaaaatggg ttcttatcaa
      361 tatgaaatta actataagga gataggcgaa tgaatattat tgatcaagtg aaacaaacat
      421 tagtagaaga aattgcagca agtattaaca aagcaggatt agcagatgag attcctgata
      481 ttaaaattga agttcctaaa gatacaaaaa atggagatta tgctactaat attgcgatgg
      541 tactgactaa gattgcaaag cgtaatcctc gtgaaattgc tcaagcgatt gttgataact
      601 tagatactga aaaagcacat gtaaaacaaa ttgacattgc tggtccagga ttcattaatt
      661 tttacttaga taatcagtat ttaacagcaa ttattcctga agcaattgaa aaaggtgatc
      721 aatttggaca tgtaaatgaa tcaaaaggtc aaaatgtatt gcttgagtat gtttcagcta
      781 accctacagg agatttacat attggtcatg ctagaaatgc agcagttggt gatgctttag
      841 ctaatatttt aactgcagct ggctataatg taacacgtga atattatatt aatgatgctg
      901 gtaatcaaat tactaactta gcgcgttcga ttgaaacacg tttctttgaa gctttaggtg
      961 acaatagtta ttcaatgcca gaagatggct ataatggcaa agatattatt gaaataggta
     1021 aagatttagc agagaaacac cctgaaatta aagattattc tgaagaagca cgtttgaaag
     1081 aatttagaaa attaggcgta gaatacgaaa tggctaaact gaaaaatgat ttagcagagt
     1141 tcaatacgca ttttgataat tggtttagtg aaacatcttt atatgaaaaa ggcgaaattc
     1201 ttgaagtttt agctaaaatg aaagaattag gttatacgta tgaagctgat ggcgctacat
     1261 ggttacgtac aactgatttt aaagacgaca aagacagagt attaattaaa aatgacggta
     1321 catatacgta tttcttacca gatattgcgt accacttcga taaagttaaa cgtggtaatg
     1381 acattttaat cgatttattt ggtgctgatc atcatggtta tattaaccgt ttgaaagcat
     1441 ctcttgaaac gtttggtgta gatagtaatc gtttagaaat tcaaatcatg caaatggttc
     1501 gtttaatgga aaatggtaaa gaagtgaaga tgagtaaacg tactggtaat gcgattacat
     1561 taagagaaat tatggatgaa gttggcgttg acgctgcacg ttatttctta actatgcgta
     1621 gtcctgatag tcactttgat tttgatatgg aattagcgaa agagcaatct caagacaatc
     1681 cagtttacta tgctcaatat gcacatgcgc gtatttgttc aattttaaaa caagcgaaag
     1741 agcaaggaat tgaggtagct gctgcgaatg attttacaac gattacaaat gaaaaagcga
     1801 tcgaattgtt gaaaaaagta gctgatttcg aacctacaat tgaaagtgct gctgagcata
     1861 gatcagcaca tagaattact aattatattc aagatttagc ttctcatttc cataaattct
     1921 ataatgctga aaaagtgtta acagatgata ttgaaaaaac aaaagcacat gttgctatga
     1981 ttgaagcggt cagaattaca ttgaaaaatg cattggcaat ggtcggtgta agcgcacctg
     2041 aatcaatgta agaacattta tatacactcc aacgtagagt ttctcgaaag atactttgtg
     2101 ttggagtgtt tttttatttg gtatgtgaca tattggggaa tgcttagtat gtgaataagg
     2161 ttaaaggaat acagttggtt tgcgcagcaa ctgcaaaaga gcccctaatc gctaaagctc
     2221 aaggggggaa aggaatacag ttggcttgct tgcgcacaac tgcataagag cccctaatcg
     2281 ctaaagctca aggggctcta aaaggaggat gtaaatgtta ggaactgatg aattgtataa
     2341 attgttatat aggcatatgg gacctcaaaa ttggtggcct gctgataatg acattgaaat
     2401 gatgttaggt gcaattttag ttcaaaatac tagatggcga aatgcagaaa ttgcattgaa
     2461 tcagattaaa gaacacacgc attttaatcc aaatcatata ttagaactac ctattgaaac
     2521 gttacaatca ttgatacgtc caagtggctt ttataaaagt aaatcactga cgattaaaac
     2581 attattaaca tggttagcac gatatcattt caattatcaa gagattaatg agcgatataa
     2641 aggtgaatta agaaaagaat tattatcttt gaaaggtatt ggaagtgaaa cagcagatgt
     2701 cttacttgtt tatatattcg gacgtattga atttattcca gatagctata caagaaaaat
     2761 atataataaa ttaggatatg aaaacactaa aagttatgat caattaaaaa aagtagtcac
     2821 attaccaaat cattttacaa atcaagatgc taatgaattt catgctctgt tagatgtatt
     2881 tggtaaacat tactttagag ataaagatat aaagaattgt gattttttag aaccttactt
     2941 taaaaagtaa acgctgtgaa gttagataga tgagtttata tgaaatataa aaaataattt
     3001 actattttct tttagtatgt ggacttatat aataaataga agcatataaa gaaaaaagtt
     3061 gtttgtttgt gcagcaactg cataaaagcc tctaatgact aaaggtgaag aggaacgcag
     3121 ttggatgcta ccgcacaact gcataaatcc ctctaatcgc taaagcgaaa agtgggatta
     3181 aaggaacgca gttggatgta accgcacaac tgcataaaag cctctaatca ctaaagttca
     3241 agaggcttta aaaaggagat gtgataatgt gaagaaatcg ttaattgctt ttattttgat
     3301 ttttatgctt gttctgagtg gctgtggtat gaaagataat gataaacaag gtagcgacga
     3361 taatggctcg tctaaatcgc cgtaccatag aattgtttcg ttaatgccta gtaatactga
     3421 aattttatat gaattaggat taggtaaata catagttggt gtttcaacgg ttgatgatta
     3481 tccaaaagat gtgaaaaagg gtaagaaaca atttgatgct ttgaatctaa ataaagagga
     3541 acttttaaag gcaaagccag atctaattct tgcgcatgag tcgcaaaagg caactgctaa
     3601 taaagtattg tcatcattag agaaacaagg catcaaagta gtgtatgtta aagatgcaca
     3661 atcaattgat gaaacttaca acacatttaa gcaaattggg aaattaacgc atcatgaaaa
     3721 gcaggctgaa caacttgttg aggaaactaa ggataatatc gataaagtca tagattcaat
     3781 tcctgctcat cataaaaaat caaaagtatt tattgaggtt tcatcaaagc ctgaaatata
     3841 tacagcaggg aagcatacat ttttcaatga tatgttagaa aaattagaag cccaaaatgt
     3901 ttatagtgac attaatggtt ggaaccctgt aacgaaggaa agtattatta aaaagaaccc
     3961 agatatatta atttcgacgg aagctaagac aagatcagat tatatggata tcatcaaaaa
     4021 aagaggtgga ttcaataaaa ttaatgctgt caagaataca cgtattgaag ttgtaaatgg
     4081 tgatgaagta tcaagaccgg gtccacgtat tgatgaagga ttaaaagaat taagagatgc
     4141 aatttataga aaataaacca ttctaattat gccccttatt gctacatgta ataaaaatta
     4201 catgtttgag ataaggggtt ttaaaatata tttagtgaat gatagcaacg cgagtatgtg
     4261 attgctataa tgaatgtaat tatcgatgaa agaaaagaga atgctatgac atttaataaa
     4321 gtattattga gctggatagt catattgatt ataacaacta gcatatatct attttggcag
     4381 ttgggggata tcaatgatgt atttaaccag tctattttaa tcaatgttag attaccgaga
     4441 ttattagaag cattgttgac aggtatgata ttaactgttg caggtcttat atttcaaaca
     4501 gttttaaata atgcattggc agatagcttt acattaggat tggcaagcgg tgctacattt
     4561 ggttcaggat tagcattatt tttaggatta acaacgttat ggattcctgt attttcaata
     4621 acatttagtt taataacatt aataactgta ttagtcatta cgtcggtatt gagccaaggc
     4681 tatccagtta gaatcttaat attaagtggt ttaatgattg gtgcactatt caattcactt
     4741 ctatattttt tgattttatt aaaacctcgc aaattaaata caattgccaa ttatctgttt
     4801 ggtggttttg gtgatgcaga atactcaaat gtatccataa tagcaatcac atttatcatt
     4861 gcactgtttg gtatatttat cattcttaat caactaaagt tattgcaatt aggagaacta
     4921 aaaagtcagt cactaggctt aaatgttcaa ataattacat atatcgcgtt atgtatagct
     4981 tctatgataa cggcgataaa tgtcgcatat gttggcataa ttggattcat tggtatggtg
     5041 ataccacaac tcattagaaa atggcagtgg aaacaatcat taggaagaca attggctttg
     5101 aatattgtaa ttggaggaca aataatggtt atggcagatt ttattggtag ccatatattg
     5161 tcacccgtac aaataccggc aagtattatc attgcattaa ttggtatacc agtgttattt
     5221 tacatgctaa tatctcagtc gaaacggtta cactagcaca tgacatttgc taaaataaaa
     5281 ataactataa acataaagag ggcataagcg atggatttga atcaaattaa agcagttgta
     5341 tttgatttag aaggtacgtt gttggacaga gttaaatctc gagagaaatt tatcgaagag
     5401 caatatgaac gatttcatga ctacttaatt catgttcaac tggcagattt taaaaaagca
     5461 tttattgagc tagatgacga tgaagataat gataaacctg atttatataa agaaatcatt
     5521 aaacgtttcc atgtagatag gttaacttgg aaagacttat ttaacgattt tgaaatgcat
     5581 ttttatcgtt atgtatttcc ttattacgat actttgtata cactagaaaa gctatcgcaa
     5641 aaaggctttc aaattggtgt tatcgcaaat ggtaaatcta agattaaaca atttcgatta
     5701 cattcacttg gtttgatgca tgttattaat tatttatcaa catcagaaac agttggtttt
     5761 cgtaaaccac atcctaaaat ttttgaagat atgattgatc aactaggggt attacctgag
     5821 caaattatgt atgtcggtga tgatgcattg aatgatgtag ctccagcacg agctatgggc
     5881 atggttagtg tatggtataa acaagaagat gcagaaattg aaccactcga agaagaagtt
     5941 gattttacaa ttacaacagt ggaagaatta ttaaccattt taccaataaa aattgataat
     6001 aaaggagaaa attatggatc tatttactag aaaagatgga acatcgatac attacagtac
     6061 attaggtgaa ggctatccta tcgtattgat tcatactgta cttgataatt attctgtgtt
     6121 taataaatta gcagcagaat tagcaaaatc atttcaagtt gtgttaattg atttacgtgg
     6181 acatggctat tctgataaac ctcgtcacat tgaaataaaa gatttttctg aagatattgt
     6241 tgaattactt aaatatctat acattgaaga agttgcattt gtatgccatg aaatgggtgg
     6301 aatcattggt gcggatattt cagtacgtta tcctgaattt acatcatcac ttatgttggt
     6361 aaatccaaca tctattgaag gtgaattacc ggaagaacgt ttatttagaa aatatgccca
     6421 tattattcga aactgggatc ctgaaaaaca agataaattt ttaaataagc gtaagtatta
     6481 tcgtccgaga aaaatgaatc gattcctcaa acatgtcgta gatacaaatg aaatatcaac
     6541 taaagaagaa attcaagcag ttaaagaggt attcaaaaac gctgatattt ctcaaactta
     6601 tagaaatgtc gtagtaccga caaaaattat tgcaggagaa ttcggtgaaa gaacaacaag
     6661 attagaagct aaagaagtag ctgatttaat ccaaaatgcg gactttgaag tatatcaaga
     6721 atcaagtgca ttcccatttg ttgaagagca agaaagattc gttgaagata cagcttcatt
     6781 tatcaacaaa catcacgatg aaaagcatgt ttaattttta gtgataagtg aaatataaaa
     6841 gtagtaatta ttatttgtaa tataattgta atatgactgt tgttttagaa atgattgttg
     6901 tataatggtt gtgagcacaa gacgatataa ataaaaggag agagtgctta tgaaaaagct
     6961 actaacggca agtataattg catgttctgt tgtaatggga gtaggcatag cgaacactag
     7021 tgccgaagca gcaagtggca actctattga tactgttaaa caattaatta agggtgacca
     7081 gtcattagaa aatgtgaaaa ttggcgaatc tattaaagat gttttaacta agtacaaaaa
     7141 tccaatgtat tcttacaatg aagatggaac tgaacattat tacgaattcc atactaaaaa
     7201 aggtatgtta ttagtaacta ctgatggtaa gaaaaacaat ggtaaagtaa ctcatatttc
     7261 aatgatgtat aatgatgcta atggtccaac atatcaagct gttaaaaatt atgttggcaa
     7321 agcagtaaca catacggaat atagcaaagt tgctggtaat tttggatata ttgaaaaagg
     7381 caaaacgact tatcaatttg cctcagcacc aaaagataaa aacataaaat tatatcgtat
     7441 tgatttggaa aaataataat taaattaatc gtgcttaacc gacaagttaa atcgcaatac
     7501 ttgtcggtta ttttattaca tggcataata cagtgaatat taatttttat gatttgcgaa
     7561 ggggatatta aaccaataca ataaataaag atgtttcatt ttaaaggagt actaccaatg
     7621 cgtattaacg ataaaatttt actagaaaat atagaagatt actttaatca taaaggttta
     7681 tcaccgcatt tgattgatga tattaaagag aaagtaatta ctgatataaa aaattctgaa
     7741 aagaaagatc aagattatat tgaatataaa agaaaatctc cagcacaaat catattaatg
     7801 attcaaagga atttgtttgc tttacaaatg aatccagtta ttttctttat tataaatttc
     7861 attctcatat cctatttata tgataaacag tatgttcagt ttcaagctat tactggaatg
     7921 agtctatttt attgtttagt gatttttcca atgactattg ttgtatacct aagggtgtct
     7981 caaaagaatt acttgcgtag taataaaata gaaatggtta tgggtacaat tatcgctatc
     8041 atatccctgt tattaattat attacaagca tttaatatta cttggggcgt tataccaatt
     8101 acaaattttg gacatcaatt tttctttttc attggtatta ttttagtaat tgccggcata
     8161 ttttataagc gacttgagtt ttcgggaatc ggcttattat tttgtcaaaa aaccgtagat
     8221 gcaatgattc ataatccaca atcagcccag actttttcat taattatatg gatattatta
     8281 gtagttctag ttatatattt cacaattaga ttatcttcac gtacaagatt ataaatatga
     8341 taaaactatt cacttgatta attgtattaa ttgagatgaa tagttttttt attgttggaa
     8401 taacttttgg taatttataa ataatttaaa aaaattgttt ataaaatgga agcgtacata
     8461 gaatgaaggt tgggtatata gtttattgag ggaggtgtca caatgaataa agtcacaatt
     8521 aatcctcaaa tccaattaac ttatcaaatt gaaggtaaag gggatcctat aatattactt
     8581 catggattgg atggtaattt agctggattt gaagatttgc aacatcaact agcatcatca
     8641 tataaagtaa ttacctacga tttaagaggt catggcaagt cttctaaaag tgaatcatac
     8701 gatttaaacg atcacgttga ggatttaaaa attttaatgg agaagttaaa tattcatgag
     8761 gcacatattc taggacatga tttaggtggg gtagttgcta agttatttac agataaatat
     8821 gcttatcgtg taaaatcatt aactaccatt gcatcgaaga aagatgactt aatacacagc
     8881 tttactcaat tgttaattca atatcaagat gatatagcgg gttttaataa gtctgaagcg
     8941 tatattcttt tattttctaa attgtttaga aatcaagaga agacgatgaa atggtatcaa
     9001 aaacaaagaa tatatagcat taagtctgag gatgatagtg cggtggcaat tcgttcatta
     9061 attttgcata aagatgaacc tatgtattta aaaaaacgta catgtgtacc tactttgtta
     9121 attaatgggg aacatgatcc tttgattaaa gataagaatc attttaaatt agaagcgcat
     9181 tttttaaatg taacgaaaaa aatcttcgaa cattcaggac atgcaccgca tattgaagaa
     9241 ccagaagcat ttctaagtta ttatctaaat tttttaaaaa gcgtatcata atatgtgata
     9301 tataaagcta gggcataaag cccttaggca atgtgaaaaa gctgattact attcattatt
     9361 tgatagaaat cagctttttt gaaatgtatt tgatatatac tgctcgttaa gcggctatct
     9421 tccttatatt aagtgccatt agtgcaaaac cgcttaacaa ttaggtaaaa agagcataaa
     9481 aaaaggaagt ttaatagaat gtatcatcta tcaaacttca ccaaattgcg ctaaacaaaa
     9541 ttatagttca atttcgttgt ttgcttcagt gattcgttta tttactcgac tcaataatga
     9601 ttcgattttt ttacgttgtt gtgcattaac aagaattaat acagttcttt catcatgctc
     9661 attacgtttt ttatcgaagt aatcttcttg agataaaatt ttaactgctt taacaacttg
     9721 tggttgtttg tagtttaaat gattaataat atctttaaga tagtattctt tctctttgtt
     9781 ttcgctgatg tatgtcaata cagcgaattc ttcaaagcta attgaaaatt cctttttaat
     9841 taaacttttt aatttgtcag cataagtgac cattgataac aactcaaagc aatcattgat
     9901 ttttgtaatt gccatggtta aaacctccct atttgatgca tcttgctcga tacatttgcc
     9961 cgataatata ttgatatcta atctttattt attatagata tgttagtcat aattttgcat
    10021 taaataagtt ttattaaata tatttaatgc tctattattt agttaattat aactaattaa
    10081 aaatgagaag taaacaaaaa agtgtttata aaacaaatta ttcattagac acagtgattg
    10141 tatttctggg ttagcatttg gattagtcaa aaagatcagc attttttatt atttacctta
    10201 atttaatcgt cagtacaagg taatttattt aatgaaacga tttagcgcaa ttaaatgttt
    10261 caatattcaa tgtgtatcta aagtgattct ttgtcatagc ttaacgcttt caagttaact
    10321 tcttctaata tttataccca tttttcgaac ttttatcact aaaaaagaaa aatttttata
    10381 atttttttaa tttagtaatt cccagtgcag ttttaatgat aagaaaaata cactatttta
    10441 atgacaaaag taaaactata gcactttaaa gtaaatgtat agtaatccga ttgataacaa
    10501 gtaatcaata ttagaaagta cgattatggt tcataattta aagcatcttc aatctttaaa
    10561 gtcgactcaa tgaatgaatt aaagttgtac attttgactt atttttaatt aattgaataa
    10621 ccaactatga aatattagca cgaataacat aaattttgct attaatttgg tgtaaattat
    10681 taaaaaataa cattaattta ggtcttaaga atgagttgac tatcgaattt gctaaattaa
    10741 aatttgttgt gtcaaagcat actacccata ttagtactta ttaaaactaa tttagaccac
    10801 aaaaaaatag cttatcactt ttaaaaataa ttacaagtta aaatgataag ctaaatgtta
    10861 aattaaaata atcttaaaaa tataatccct gtcaatatac acaacaggcc tatagttttt
    10921 cttgatgtca tagctatttt aggtgaacca aatagggcaa agtgatcaat gagtatgccc
    10981 ataagtattt gaccaaacat accaataagt gttgttaatg ctgcacctaa atggggcatt
    11041 aaaattatat tagatgttac aaaagccatg cctagtatac cgccagtaaa atagataggt
    11101 tttaatttac cgaattttaa atgacttgtt tttagtttta aagaacgatt aaaaatagcg
    11161 gttaaaatca atagcgttat tgacccaatt gtaaatgata ctaatgatgc aaaggctggt
    11221 gaatgagtat gacttgctaa taaactgtta attgtcgttt gaataggtgg aaagaaacca
    11281 aaaataaatc ctaagagaag ccaaaacaat aaatactttt gatcagttag taataaatta
    11341 ttcttgttaa attgattcat tatgacgata ccgataatga gtaacaatac tccaattgct
    11401 ttaattaaat taaaatcatg aattgtagca ccaaataatc caaatgtatc aataatgaca
    11461 cccataataa tttgacccgc aactgttgca attacagtta atgttgcacc taattttggc
    11521 aataacaata aattgccagt taaatagcca acaccgagta atccgccaac gaaccaagta
    11581 tagttgaatg tttgattact aaaaaagtgt aatgagaaaa cgtcaggatt tataaaaata
    11641 tttaagacga ttaagcatat agtacctact gaaaatgaaa taaatgatgt atagaaaggt
    11701 gatttagtat acagtgatag tcttgaattg acagatgttt ggataggaat aagcattcca
    11761 actaggacac caatgatata gaaaagaagc atatgaataa agtctcactt tccaaagtta
    11821 ataataaaaa acatatgtat agattatcac tatagagaaa aaatacaact tatttttaaa
    11881 agagataaaa gtaaagcgag tgccataata ataccccagt caatcaagaa gaaagactgg
    11941 ggcgttaatg attatattca atgaatgact acctatgttt gagactattt ctttgcttca
    12001 aataaaacgg tcgtcgtttt atcaataatt tgagcagaat gtactttggt gatagcgcca
    12061 ctattagact ttaaaatatt taaatctact aatgcattca tactatcacg tgcaacttgc
    12121 tcggttacga tgttgtttaa ttgtggtaat tgcaatttga caggtttgtc taatgaagat
    12181 ttaaaactta attcaagtgt tttggtcatg aaagtgtcct cctgattaaa ttgatacaga
    12241 tttgatgact tcgatgttat tgtatgtttc tccagtaagt cgctcgataa gtttgctaaa
    12301 tgttttaatt tggtcgtttg atacttcagg attaatattg gcgaatcgac gtttaaattc
    12361 tgtttgttta ccatttgtat ctagttttgt aaatgataat acaattgtaa tgtgatttgt
    12421 tttactcata ttttaaaacc tcctttcaca ctatatatcg aaagaaaaaa ataattgggt
    12481 taattttatt attcttgttt aaattctata aaaaaggtta taattatatg tgacttaaat
    12541 acttaatgtt tatgattaag acacttaaag caaggaggga catatgaaca aggtagaagt
    12601 aattaaatgc aatgatgata ttaataaaat gtatgacgcg ctcaaagctc gatctgatag
    12661 ggattatttg ttttttaaat tagctataca tagtggtatg aaaataacag aattattaac
    12721 aattactgtt gaagatacta aacgactgat agaaaagggg acactaagcg aattgtgtaa
    12781 agcccattat cactcattga ttaaaattag attgccagaa acattatcta atgagttact
    12841 tcattatatt gaaaacaaaa gtctgtcaaa tgaagatgta ttatttcaat cattgagaac
    12901 taatcaagta ttatctaggc aacaagccta tcgtattatt catcaagcag caattaaggc
    12961 tggcatagag aatgtaggac taacgacatt gcgtaagaca tttgcgtatc atgcatatca
    13021 aaagggcata cctattcctg tcattcaaaa gtatttaggg catcaatcag ctatagaaac
    13081 attgaatttt atcggtttag aaaatgaatg tgaacacagt atttatattt cattacaatt
    13141 ataacgaaaa aggaggctaa taatgagttt ggtttatcta ttaattgcta tactagtaat
    13201 tatggcgatg atattattaa catctaaacg tagagcaatg gctaaatatg caggatacat
    13261 agcgctgact gcacctgtaa tagcatctat ttattttttg ttacaagttc catcagtaat
    13321 taaacagcat tatttgtctg tttccattcc gtggatgacc tcattagata ttaatgtaga
    13381 tttacgttta gatggtttga gtttaatgtt ttcccttatt atttccctta ttggaatcgc
    13441 tgtattcttc tatgcaacac aatatttatc ctctcgtaaa gataatttac caaggtttta
    13501 cttatactta acgctattta tgtttagcat gctcggtatt gtattagctg ataatacaat
    13561 attaatgtat gtgttttggg aactaactag cgtatcatca tttctattaa tttcatattg
    13621 gtataacaac ggagatagtc aatttggtgc gatgcagtca tttatgatta cagtatttgg
    13681 tggtttggca ttattagttg gttttatcat gttgtatatc atgacaggaa cgaataacat
    13741 cacagagata ttaggacaag cagatcatat taagaaccat gctttattta tccccatgat
    13801 tatcatgttt ttattaggtg catttacaaa gtctgcacaa ttcccatttc atatttggtt
    13861 accaagggca atggcagcgc ctacacctgt aagtgcatat ttacattcag ccactatggt
    13921 taaagctggt atctttttat tacttagatt tacacctttg ttaggtctaa gcaatatgta
    13981 tatttatatc gtaacctttg tcgggttaat aactatgtta tttggatcaa tcacagcttt
    14041 aaaacaatgg gatttaaaag gaatcctagc atactctaca atcagtcaac ttgggatgat
    14101 tatggctatg gtgggtatag gtggcggata tgctcaacac caacaagacg caatagcatc
    14161 tatttatgta tttgtgttat ttgcagcatt gttccatctt atgaatcatg caattttcaa
    14221 gtgtgcgctt ttcatgggtg tagggatttt agatcatgaa gcaggttcaa gggacatacg
    14281 aattttaagt ggcatgcgtc aattatttcc aaaaatgaat cttgttatga tgattgcggc
    14341 gctatctatg gctggtgttc catttttaaa tggattttta agtaaagaaa tgttcttaga
    14401 tgctttaaca caaactggac aattgtccca atttagtttg atttcaatga ttatcatcgt
    14461 atgtatgggt gttattgcta gtatttttac attcacatat gcactttaca tggttaaaga
    14521 agtattttgg actaaatatg attctaaagt gtttactaaa aaaaatatcc acgaaccatg
    14581 gttgtttagt ttaccctctc ttatattaat ggtgctagta cctgtaatct tttttgtgcc
    14641 aaatatattt gggaagggga ttatcgttcc agcattaaga ggtgtatcag gtggtaatca
    14701 tcaaattgat ccattggtac cacatgtttc gcaatggcat ggatttaaca taccgcttct
    14761 tttaaccatc atcattattt tattgggtag tgtactagca atcaaagtag attggaaaaa
    14821 agtgttcaca ggtaaaatta gacaaatttc agtttcaaaa gggtacgaaa tggtttaccg
    14881 acaatttgaa aagtttacta cgaagcgatt taaacgtgtt atgcaagatc gtttaaatca
    14941 atacattatt atgacactgg gcatatttat ggtaatcatc ggatacggtt atattcgtat
    15001 aggactacca aaagttcatc agttacatgt ttccgaattt ggaccgttag aagttatatt
    15061 agcaattgtt acagttatca ttggtttgtc attaatattt atacgtcaac gactaaccat
    15121 ggtcatttta aatggggtca tcggttttgt tgtaacttta ttctttatag caatgaaagc
    15181 acctgattta gcattgacac aacttgtagt tgaaacgatt acgacaattt tatttattgt
    15241 tagcttttca agattaccca acgtaccaag gtctaaagtt aataaaaaac gagaaataat
    15301 taaaatttca gtatctctta tgatggcgct gattgttgtg tcacttattt tcatcgcgca
    15361 acaagcagat ggattggcat cgatttctaa tttttatctt agagcggata aattaacagg
    15421 tggcaaaaac attgtgaatg cgatattagg agactttaga gcattagata cattgtttga
    15481 aggtttagta ttaattatta ctggattagg tatttacaca ttattaaatt accaagatag
    15541 gaggggacaa gatgaaagag aatgatgtcg ttttaagaac ggtcacgaaa ctcgttgtat
    15601 ttattttatt gactttcgga ttctacgtct tcttcgctgg ccacaataac ccaggtggtg
    15661 gctttatagg tggtttaata tttagttccg cgtttatttt aatgtttcta gcttttaatg
    15721 tcgaagaagt attagaaagc ttaccgatag attttagaat tttaatgatt attggtgcat
    15781 tagtttcatc tattactgcg ataatgccaa cattttttgg aaaacctttt ttgtcgcaat
    15841 atgaaacaac attgacacta ccaattttag ggcacattca tgtaacgaca ataacactgt
    15901 ttgaattggg cattttgttc tcggttgtcg gggttattgt aacggtgatg ctgtctctta
    15961 gtggaggtcg atcatgaatt taattttatt actagttata ggatttttag tatttatagg
    16021 gacatatatg atattatcta taaatttaat tcgtattgtc ataggtattt caatttatac
    16081 acatgctggt aaccttatta tcatgagtat gggcacttac ggctctaata aatcagaacc
    16141 gctgattaca ggtggaaatc aattgtttgt cgacccactg ttgcaagcta ttgtattaac
    16201 tgcaatagtt ataggttttg ggatgactgc gtttttactt gtcctcgttt atagaactta
    16261 taaagtaact aaagaagacg aaattgaagg cttaaggggg gaagatgatg ctaagtaatt
    16321 tgttaatatt accaatgtta ttaccattcc tttgtgcctt aatccttgta tttttaaaaa
    16381 ataatgaccg tatttctaaa tatttatact taggtacaat gactatcacc acaattattt
    16441 cattaatgct attaatttat gttcagcggc atcgtccaat tacattggat tttggtggtt
    16501 ggactgcacc atttggaata cagtttttag gagattcgtt aagtttaatc atggttacaa
    16561 ccgcttcgtt tgtgattact ttaattatgg catatggatt tgggcgtggc gaacataaag
    16621 caaatcgtta tcatttacct tcatttatat tatttttaag tgttggtgtg ataggctctt
    16681 ttctaacatc agatttattt aatttatatg tcatgtttga aattatgtta cttgcgtcat
    16741 ttgtactcat tacacttgga caatctgtag aacaattacg tgccgcaatt atttatgttg
    16801 tcttgaatat tattggttca tggctattct tattaggtat aggtttactt tataaaacag
    16861 taggtacatt aaacttttca catattgcaa tgcgtttgaa tgacatggga gataatcgca
    16921 ctgttacaat gatttcatta atcttcttag tcgcttttag tgcgaaggca gcgctggtcc
    16981 tttttatgtg gctacccaaa gcatacgcag tgttaaatac tgagcttgca gcattatttg
    17041 cagcgttaat gaccaaagtg ggcgcttatg cattgattcg cttctttaca ttattatttg
    17101 accaacataa tggtcttata catccattgc tagtaacaat ggcagctata actatggtca
    17161 tcggcgctat tggtgtcatt gcttataaag atattaaaaa gattgcagct taccaagtca
    17221 taatctcaat aggatttatc attttaggtt taggaacaaa cacgtttgca ggtattaatg
    17281 gtgcaatatt ttatttagta aatgatattg ttgtaaaaac attgctattc tttattattg
    17341 gtagtttagt ttatattact ggttatcgac aatatcaata tttaaatggt ttagcaaaaa
    17401 aagaaccatt ctttggtgtt gcgtttatta taatgatttt tgctattggc ggcgtgcccc
    17461 catttagtgg ctttccgggg aaagtactta ttttccaagg tgcattgcaa aatggcaact
    17521 atattggact agcgttaatg attattacta gtctaattgc tatgtacagc ttgtttagaa
    17581 tacttttcta tatgtatttt ggagataaag atggcgaaga agttcatttt aagaaaatcc
    17641 cacaatatcg taaaggtatt ttaagtattt tagtagttgt ggttatcgca atcgggattg
    17701 cagcgcctgt gctgttaaaa ataactaatg atgcaacaga acttaatacg aacgatcaat
    17761 tatatcaaaa gcttgttaat ccacatttga aaggagagga ataaatgaat caaattgttt
    17821 taaatattat cattgcattc ttatgggttt tatttcaaga tgaagatcat tttaaattct
    17881 cgactttctt ttctggatat ctaattggtt taattgtcat ttatatatta cacaggtttt
    17941 tcagcgatga tttttatgtt agaaaaattt gggtagctat taaattttta ggtgtttatt
    18001 tatatcaatt aataacatct agcattagca cgattaatta tattcttttt aaaacaaaag
    18061 atatgaaccc tggattactt tcttatgaaa caagactaac aagtgactgg gcaataacat
    18121 ttttaacaat tttaattatt ataactccag ggtc
//
LOCUS       DAMZPP010000050        17958 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMZPP010000050 DAMZPP010000000
VERSION     DAMZPP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17958
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.050"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            108..821
                     /locus_tag="POH73_001264"
     CDS             108..821
                     /locus_tag="POH73_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDI6887229.1"
                     /translation="MTHNIEKRINKLKTSGNPKFKKLDSDIHYLLKRFEGEKNHKGFY
                     PKFKQGEIVFVDFGINVNKEFSNSHFAIVMNKNDSNTEDIVNVIPLSSKENKKYLKMN
                     FDLKWEYYLRLFLNLISAQNNPAILKEVFDKKYQKNNTEFITKDYFSEFISDSLEIEN
                     KLNKIDRNINNIVSAIDKVKKLKGNSYACINSFQPISKFRIRKVLPQKIKNPVIDSSD
                     IMLLINRINNNILQIPDIR"
     gene            1012..2049
                     /locus_tag="POH73_001265"
     CDS             1012..2049
                     /locus_tag="POH73_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI6887230.1"
                     /translation="MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLHAINNALKSAC
                     RVNNIPIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            2100..2930
                     /gene="sph"
                     /locus_tag="POH73_001266"
                     /pseudo
     CDS             2100..2930
                     /gene="sph"
                     /locus_tag="POH73_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(3187..4206)
                     /locus_tag="POH73_001267"
     CDS             complement(3187..4206)
                     /locus_tag="POH73_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leukocidin/hemolysin toxin family protein"
                     /protein_id="HDI6887231.1"
                     /translation="MIKQVCKNITICSLALSTALTVFPASSYAEIKSKITTVSEKNLD
                     GDTKMYTRTATTSDTEKKISQSLQFNFLTEPNYDKETVFIKAKGTIGSGLKILNPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNSTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRG
                     GLTGNITKEKNYSETISYQQPSYRTLIDQPTTNKGVAWKVEAHSINNMGHDHTRQLTN
                     DSDDRVKSEIFSLTRNGNLWAKDNFTPKNKMPVTVSEGFNPEFLAVMSHDKNDKGKSR
                     FIVHYKRSMDDFKLDWNKHGFWGYWSGENHVDQKEEKLSALYEVDWKTHDVKLIKTIN
                     DKEQK"
     gene            complement(4228..5283)
                     /locus_tag="POH73_001268"
     CDS             complement(4228..5283)
                     /locus_tag="POH73_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leukocidin family pore-forming toxin"
                     /protein_id="HDI6887232.1"
                     /translation="MKNKKRVFIASSLSCVLLLLSAANTEANSANKDSQDQTKKEHVD
                     KAQQKEKRNVNDKDKNTPGPDDIGKNGKVTKRTVSEYDKETNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHRNETNASWLKYPSEYHVDFQVQRNPKTEILDQLPKNK
                     ISTAKVDSTFSYSLGGKFDSTKGIGRTSSNSYSKSISYNQQNYDTIASGKNNNRHVHW
                     SVVANDLKYGNEIKNRNDEFLFYRNTRLSTVENPELSFASKYRYPALVRSGFNPEFLT
                     YISNEKSNEKTRFEVTYTRNQDILKNKPGIHYGQPILEQNKDGQRFIVVYEVDWKNKT
                     VKVVEKYSDQNKPYKEG"
     gene            5715..6938
                     /locus_tag="POH73_001269"
     CDS             5715..6938
                     /locus_tag="POH73_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDI6887233.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLKDLFDKYDIKS
                     EILKVNEHRANFVAEIGSGSPILALSGHMDVVDAGNQDNWTYPPFQLTEKDDKLYGRG
                     TTDMKGGLMALVIALIELKEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDG
                     LMIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNQFKEKYAE
                     LKKNDTKHELDVAPMFKSLIGKDISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINNVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVDKDDIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
     gene            7375..8682
                     /locus_tag="POH73_001270"
     CDS             7375..8682
                     /locus_tag="POH73_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDI6887234.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNAGFALFKNNLIDYSNDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLH
                     SKLVLTTTSILIIIGAITFFLLEQFNTMQNMGLVEKIGNSFFQSITTRTAGFNSIDIA
                     SINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISD
                     KYIKLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYH
                     GITKIIIIFVMLCGKVGLLTLLRTFIPPKSPKNYRYTKGQIYL"
     gene            complement(9260..9601)
                     /locus_tag="POH73_001271"
     CDS             complement(9260..9601)
                     /locus_tag="POH73_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887235.1"
                     /translation="MLDKISNTLKKKLYQKGETHSGNDNLIIQGYLIAQIEKNYSEEA
                     LATKSINANDLLTLAYVKVLEGANIFGGSYIWIEYEDVDRLREFYRKFGFTEIKDHTS
                     ENNLKMAILKI"
     gene            complement(9591..9887)
                     /locus_tag="POH73_001272"
     CDS             complement(9591..9887)
                     /locus_tag="POH73_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887236.1"
                     /translation="MTVKVISLSELLTGDKQEVKRKIPSVLNILNSFETISISGSESA
                     HDVDLFLKNKSIAFDRQNLSRTHLVFSQFKNKQILVGYFTISNKPLVFYKTYVR"
     gene            complement(9884..10063)
                     /locus_tag="POH73_001273"
     CDS             complement(9884..10063)
                     /locus_tag="POH73_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887237.1"
                     /translation="MATISITTDYKFTKKSAQKLIDAMEINENNSNVNKTNIKATKIK
                     STCEIENLLKDYRSN"
     gene            11182..12117
                     /locus_tag="POH73_001274"
     CDS             11182..12117
                     /locus_tag="POH73_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDI6887238.1"
                     /translation="MRLQKAPLVTSGLILGLLGLGNLLKDISLVLNAICGIFALFIWV
                     HLLCTILNNFKNVKEQLNTPLVSSVFTTFFMSGFLGTTYLNTYFSDVTIITSLITPLW
                     LLCLMGIMIHMIIFSIKYLKGFSLENVYPSWTVLYIGIAIAGLTAPISGFYLIGKLSV
                     IYGFLATCIVLPIVFKRLKTYPLQTSIKPNTSTICAPFSLVAAAYVITFPKANDLIVI
                     ILLVLAQFFYFYIIFKLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLTYPEIWEW
                     LLFFEITLATLIVLRVFLGYVHFFFKTSPKASYNS"
     gene            complement(12972..13199)
                     /locus_tag="POH73_001275"
     CDS             complement(12972..13199)
                     /locus_tag="POH73_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HDI6887239.1"
                     /translation="MKQNDLAHGEFGNWLKNINLDRTQAHRFIKVSEEIKDVGTYQHL
                     GLRALSEIASLPVPVRIKVHITSNGKTKIPY"
     gene            13618..14322
                     /gene="tst"
                     /locus_tag="POH73_001276"
     CDS             13618..14322
                     /gene="tst"
                     /locus_tag="POH73_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_111772164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic shock syndrome toxin TSST-1"
                     /protein_id="HDI6887240.1"
                     /translation="MNKKLLMNFFIVSPLLLATIATDFTPVPLLSNQIIKTAKASTND
                     NIKDLLDWYSSGSDTFTNSEVLDNSLGSMRIKNTDGSISLIIFPSPYYSPAFTKGEKV
                     DLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLPTPIELPLKVKVHGKDSPLKYWPKFD
                     KKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKFEYNTEK
                     PPINIDEIKTIEAEIN"
     gene            complement(14477..15046)
                     /locus_tag="POH73_001277"
     CDS             complement(14477..15046)
                     /locus_tag="POH73_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDI6887241.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYSANSAHVTGCRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGDVPIFINIGEWDGD
                     DEDLDKTVQEVSNANPNHTVIVDDIPLED"
     gene            complement(15043..15384)
                     /locus_tag="POH73_001278"
     CDS             complement(15043..15384)
                     /locus_tag="POH73_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDI6887242.1"
                     /translation="MDKKQIKDFVCDYHKRTRSDVLIDDEINTDEFFSIGDENSNEWM
                     ADDNIDDHIVKNHLEMIVDQVANDKEFYIFDSLIQGRSFKDISNVLECSEQSVRLWYE
                     TLLDKIVEVIE"
     gene            complement(15387..15914)
                     /locus_tag="POH73_001279"
     CDS             complement(15387..15914)
                     /locus_tag="POH73_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDI6887243.1"
                     /translation="MKLLKTKNCLYYRNGDNKLSEYQLLTQFNPAFINKKIKMCEFQI
                     ESMYHMSASTTTCDEIMGVVSVSYPIEKLVIKIIETKAGLQNYKNRSINNMALLKKVL
                     NHYTEKEQKQVVKYMRSNGRYKPYNVIERLQVDLYQASIKQRSERQKQRNTAIENSKI
                     ARVNAYHQSSYVKVV"
     gene            complement(15965..16183)
                     /locus_tag="POH73_001280"
     CDS             complement(15965..16183)
                     /locus_tag="POH73_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid morphogenesis B protein"
                     /protein_id="HDI6887244.1"
                     /translation="METKYELNNTKKVANAFGLNEEDTNLLINAVDLDIKNNMQEISS
                     ELQQSEQSKQKQYGTTLQNLAKQNRIIK"
     gene            complement(16201..16779)
                     /locus_tag="POH73_001281"
     CDS             complement(16201..16779)
                     /locus_tag="POH73_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDI6887245.1"
                     /translation="MKTESYFKEYNQFVIDQQKAIQELKQERNALESKIKIDKSTYKQ
                     LIMDGQDDKADNLYQATDADEKKLKALNKRLETKKSVSKEVKYQKTIELLKHQSELSS
                     LYESEKQSALGKLKKVVDAYNEIIDEIEDINDRYEDEHQQYASIYSQEQLYDDKEARE
                     ALNGYFRENIFTSYINGNDLPYEHNNKLFLKR"
     gene            complement(16791..17132)
                     /locus_tag="POH73_001282"
     CDS             complement(16791..17132)
                     /locus_tag="POH73_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDI6887246.1"
                     /translation="MQSIAEKETYHLPTEHLQVFNVIKNTSNKYITKTKILNQLGYEY
                     NSSNERWLRRVINSLVYDYGYPIGCSYKPSERGYYIIMTEQEKQQAMRSIKKLADGSM
                     KRYEALKRIKV"
     gene            complement(17668..>17958)
                     /locus_tag="POH73_001283"
     CDS             complement(17668..>17958)
                     /locus_tag="POH73_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012990921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDI6887247.1"
                     /translation="LQTYAESNNSDVFEYLENGFDTDTLEGIQSSNTDVIANIEMLYQ
                     LATGINEPAPELVEGLRLVTEFVQDENATQEDYKALEHKLTELKSSYYSLNK"
BASE COUNT         5995 a         2935 c         2491 g         6537 t
ORIGIN      
        1 gataagttaa ccaaagaaga ttcattatga ttttactaat caaaaaacgt ctacaagtgt
       61 agacgttgaa tggtggtgag agtgtgagcg agaataaagg agaaatgatg acgcataata
      121 tagaaaaacg cattaataaa ttaaaaactt ctggaaatcc aaaatttaaa aaattagatt
      181 cagatattca ctatttactc aagagatttg aaggtgaaaa aaaccataaa ggtttttatc
      241 caaagtttaa acaaggagaa atagtttttg tagatttcgg tataaacgtt aataaagaat
      301 tctctaattc acactttgca atagtgatga ataaaaatga ttctaatacg gaagatatag
      361 taaatgttat tcccttatct tctaaagaaa acaaaaagta tttaaagatg aattttgatt
      421 tgaaatggga gtattattta agattgtttt taaatttaat tagcgcgcaa aataatccag
      481 ctatattaaa agaagttttc gataaaaaat accaaaaaaa caacacagaa ttcatcacta
      541 aagattattt tagtgaattt atatctgata gtttagaaat tgaaaataaa ttaaataaaa
      601 ttgacagaaa cattaataac atagtatcag caattgataa ggtaaaaaaa ttaaaaggta
      661 atagttacgc ttgcataaat tctttccagc cgattagtaa gtttcgcata agaaaagttt
      721 taccccaaaa aattaaaaat ccagtaatag attcttcgga tattatgtta ctgataaata
      781 gaattaataa taatatattg cagattcctg atataagatg atataatttt aatatattaa
      841 aggtttatcc tttaaaacac gtatatattc gttaccattt ttggtaatta accatgtaat
      901 cttataacta taagtggcgt ctgtatttta tacaggcgtc tttttttata caattttcat
      961 gggtagcccg cctaccctta ttattttttg ccaattttga ggagggaacg catgaaaaca
     1021 cgttgttacg atggtaaaaa atggcaatat gaatttaagt atgaaggaaa aagataccgt
     1081 aagaaaggtt ttagaacaaa gcgtgaagct aattctgctg gactagacaa gttaaatgag
     1141 ttaagaagtg gttttaatat agataactat ataactcttg aagaatactt cgaaaattgg
     1201 attaaaacgt ataaacaacc tgttgttaaa gaaaatacct accgtcatta tagaaatgca
     1261 ttacaacata tacaaaaaca taaaataggt aaaatggagt tatcaaagat aaatagacaa
     1321 gtttatcaga aattcataaa cgattattca aaagaacacg caaaagaaac tataagaaaa
     1381 acaaacggtg ctattcggtc agctttagat gacgcattat atgatgggct tatttttaaa
     1441 aatcccgctt ataaagttaa ttataaagcc ggaaaaccta cgaagtcaga acaagaaaaa
     1501 ttcatctcgg taactgaata tgaaatacta aaagatcacg tcagaaagaa gagaactcgt
     1561 tcatcattag cgctattcat aatgatttgt acgggttgtc gtgtcagtgg tgcaagaaat
     1621 ataaagattg agcatatcaa ccaagtgaaa aacactatat ttattgacga gcgaaaaacc
     1681 gatacttccc ctagatatat cagtatcgct aaatctgata tgaaacacat tatggacgtc
     1741 ataagtacat ttgcaattag ctatgatggt tacattttca aagaagccgg atctataatt
     1801 aaccttcatg ctatcaataa tgctttgaaa tcagcctgta gagtcaataa tataccaatt
     1861 attacatcgc acgcattaag acacactcat tgttcttatt tactagcaaa aggtgtatct
     1921 atacattaca tttctaaaag attaggtcat aaaaatatag caataactac atccgtgtat
     1981 tctcatttgt tagaagaaaa atttaatgaa gaggacaaaa aaacaactaa aattttagaa
     2041 agtatgtaat ttagggaccc attagggact ccaaacccaa taaatactgt tgttacaagg
     2101 tttctatgta tccaaactgg gggcaatata aacgcgctta tttaatcgga caatcttctt
     2161 atattaaaaa taatgatgtc gtaatattca atgaagcatt tgataatggt gcatcagaca
     2221 aattattaag taatgtgaaa aaagaatatc cttaccaaac acctgtactc ggtcgttctc
     2281 aatcaggttg ggacaaaact gaaggtagct actcatcaac tgttgctgaa gatggcggcg
     2341 tagcgattgt aagtaaatat cctattaaag aaaaaatcca acatgtattc aaaagcggtt
     2401 gtggattcga caatgacagt aataaaggct ttgtttatac aaagatagag aaaaatggta
     2461 agaacgttca cgttatcggt acacatacac aatctgaaga ttcacgttgt ggtgctggac
     2521 atgatcgaaa aattagagct gaacaaatga aagaaatcag tgactttgtt aaaaagaaaa
     2581 atatccctaa agatgaaacg gtatatatag gtggcgacct taatgttaat aaaggaactg
     2641 aagagttcaa agatatgctt aaaaacttga atgtaaatga tgttttatat gcaggtcata
     2701 atagcacatg ggaccctcaa tcaaattcaa ttgcgaaata taattatcct aatggtaaac
     2761 cagaacattt agactatata tttacagata aagatcataa acaaccaaaa caattagtca
     2821 atgaagttgt tactgagaaa ccgaaaccat gggatgtata tgccttccct tactactatg
     2881 tatacaatga tttctcagat cattatccaa tcaaagccta tagtaaatag cactcaacaa
     2941 aaaactcgct tcgttctaaa aagtcgaagc gagttatatt gttaaaattt gaattgacta
     3001 actttaaact gatatctcat cgttttcatt tctttaactt ccactatacc tctacatgcc
     3061 tcatatgcac ctaacaatat caagttaggt tatccataat aaaagataca gtgtctcaag
     3121 ctatctatcc caaaacaaat aaatagttac ctactacgtg atacgcagca ggcaacttgc
     3181 gaaaacttat ttctgttctt tatcattaat tgttttaatt aattttacat catgtgtctt
     3241 ccaatcaact tcatataatg ctgataattt ttcttctttt tgatctacat ggttttcacc
     3301 agaccaatag ccccagaaac catgtttgtt ccaatctaat ttaaagtcat ccattgatcg
     3361 tttataatga acgataaatc ttgatttacc tttatcattt ttatcgtgtg acattacagc
     3421 taaaaattct ggattaaatc cttctgacac agttacaggc attttatttt taggcgtgaa
     3481 attatctttc gcccataggt ttccgtttcg agttaatgaa aagatttcac ttttcactct
     3541 atcatcactg tcattagtta gttgtctcgt atgatcatgt cccatattat tgatagaatg
     3601 tgcttctact ttccaagcta cacctttatt tgttgtaggt tgatcaataa gtgtgcggta
     3661 agatggttgt tgataactaa ttgtctctga ataatttttc tcttttgtaa tatttccagt
     3721 taaaccacca cgattaattg aaaaatctcc acctgtttta tagccatacg tatatttaac
     3781 ttctcttgat tcatcttgat ttttaggcgc aaaatcagtc acatttgtac tgttgttgtt
     3841 atcatcaacg ttttgaatcg agactgaata agaaccaggc catcttaatg tactattcca
     3901 ataaccattt ggatttaaaa ttttcaatcc actgccaatt gtacctttcg ccttaataaa
     3961 tactgtctct ttatcataat ttggctcagt aagaaaatta aattgtaagc tttgtgagat
     4021 ttttttctct gtatcactcg ttgtagccgt acgtgtatac atcttcgtat caccatcaag
     4081 gttcttctcg gaaactgtag taattttaga tttaatttct gcataagaag atgctggaaa
     4141 tacagttaaa gctgttgata gcgctaaact acagattgta atatttttac atacttgttt
     4201 aatcatttgt catccgtccc ttctacttta tccttcttta tatggtttat tttgatcaga
     4261 gtatttttca acgactttaa ctgttttatt tttccaatct acttcataaa ctacaataaa
     4321 tctttgacca tctttatttt gttctaaaat tggttgtcca taatgtatac caggtttatt
     4381 tttcaaaata tcttgattac gtgtatatgt aacttcgaat cgtgtttttt catttgactt
     4441 ttcattagaa atataagtta agaattctgg gttaaatccg cttcttacaa gcgcaggata
     4501 tctatatttt gatgcaaaac ttaattcagg attttctaca gtagataatc tagtatttct
     4561 atagaataag aattcatcat ttctgttctt aatttcattc ccgtatttta aatcgttggc
     4621 aacaacagac cagtgtacgt gtctattatt atttttaccg ctcgcaattg tgtcgtaatt
     4681 ttgttggtta taactgatag atttagaata gctattagat gatgttcgtc caataccttt
     4741 tgttgaatcg aatttaccac ctaagctata agaaaatgta ctatctactt tcgcagtgga
     4801 aattttattt tttggcagtt gatctaaaat ttcagtttta gggtttcttt gtacttgaaa
     4861 atcaacgtga tattcactcg ggtattttaa ccatgatgcg tttgtttcat ttcgatgaga
     4921 ttcaaacttt aaatttgaat gaattgaccc ttgttttttg acaagtaaga cattcttgtc
     4981 atatgttgga tcatcgataa agtcgaattg taaattttgt aaaatatttg tctctttatc
     5041 gtattcagat acagtacgct ttgtaacttt accgttttta ccaatatcat caggtcctgg
     5101 tgtattttta tctttatcat tcacattacg cttttctttt tgttgagcct tatcaacatg
     5161 ttctttctta gtttggtctt gtgagtcttt attcgctgaa ttcgcttctg tatttgctgc
     5221 tgataacaat aaaagtacac atgataatga tgacgcaatg aatacgcgct ttttattttt
     5281 cattgttata accttctttc ttatatttaa tattgttgat aggtatcgac atgtgaataa
     5341 tatcacaaaa actaagaata tatatttaac tatttattaa attattttgt ttatattatt
     5401 aaatactttt ttcgatataa aattaatgtg tgtacaaagt gattaaatta gtaaatcgca
     5461 ttatttagta gttaatcacc atggatctat atgttataca tgcttaaact aaattttttt
     5521 catacaatcc gactggacga ttcacaatga aaaaaacact tactatcagt gattattaga
     5581 ctactgatac aaggtgcata ttttaagatg taatcatgaa catagcacgt cactttgtta
     5641 cttaaaatta atgtttaaat acaataatta tgtttatata aatatatata ctactttgaa
     5701 aaggtgtgag cttaatgaca acttttagtg aaaaagaaaa aattcaatta ctagcagata
     5761 ttgttgaact acaaactgaa aataataatg aaatagacgt ttgtaattat ttaaaagatt
     5821 tattcgacaa gtacgatatt aaatctgaaa ttttgaaagt taatgaacac cgcgccaatt
     5881 tcgttgcaga aatcggtagt ggctctccca tacttgcatt gagcggtcat atggatgttg
     5941 ttgatgcagg aaatcaagat aattggactt atcccccttt tcaactcaca gaaaaagatg
     6001 acaaattata tggtcgaggc acaacagata tgaaaggcgg tctgatggct ttggtcatcg
     6061 cactcattga attaaaagaa caaaaccaat tacctcaagg aaccattaga ttactcgcta
     6121 ctgctggtga agagaaagaa caagaaggcg ccaaattatt agcagataaa ggctatttag
     6181 atgatgttga tggtctaatg attgctgaac caactggatc tggaatttat tatgcacata
     6241 aagggtctat gtcatgtaaa gtaactgcaa ctggtaaagc tgtccatagc tcagttccat
     6301 ttattggtga caacgcaatt gatacactgc ttgaatttta taatcaattt aaagaaaaat
     6361 atgcagagct taaaaaaaat gatactaaac atgagttaga tgttgctccc atgttcaaat
     6421 cattgatagg aaaagatatt tctgaagagg atgcaaatta tgcatctggt cttacagctg
     6481 tatgttcgat tataaatggc ggtaaacaat ttaactctgt accagatgaa gcttcacttg
     6541 aatttaacgt aagaccagtt cctgagtatg ataacgactt tatagaatcg tttttccaaa
     6601 atatcattaa taatgtggat agcaataagc tttcactcga tattccaagc aaccatcgac
     6661 ctgtaacaag cgataaaaat agcaaattaa ttactacgat taaagatgta gcttctagtt
     6721 atgtagacaa agacgatata tttgtttcgg cgcttgtagg tgcaacggat gcttccagct
     6781 tcttaggaga taataaggac aatgttgatt tagccatttt tggaccaggt aatccattaa
     6841 tggcacatca aatcgatgaa tatattgaaa aagatatgta tctgaaatat attgatattt
     6901 tcaaagaggc ttccattcaa tatttaaagg agaaataaga ggtatactgt cagctgccct
     6961 attcgcgtgc tggcagtttt ttattgcgaa ttttaagttg atagaaatag cgatgaatat
     7021 tgtttgaatg taaatattca aatatcaaca agcatatttc cattgaatag gtgatgtaaa
     7081 actgaaacta tagcgttgat ttgtcatact taaattgcat tgtctgttga aattgggaat
     7141 ccaatttctc tttgttgggg cccccccaac ttacattgtt tgtagaattt cttttcgaaa
     7201 ttctctgtgt tggggccccg ccaacttgca ttgttcgcag aatttctttt tgaaattctc
     7261 tgtgttgggg ctcctgacta gaattgaaaa gtaatacaca tcagtaacat ttctattttc
     7321 atttatgata ttatctaatt attaatttat aatataaagg agtatgcaat taaaatgaac
     7381 aaagtccaca aacctttata tttttattta atgcttttct tctctacaac tatcataggt
     7441 gcacttttat tgtatttacc tttcactggt aaaaagccaa tatctttttt agatgcccta
     7501 tttatagctt caagtgcatt tacagttact ggcttgtccc cagttgatat aggatcacag
     7561 tttaatatac ttggtgagat agtaatacta ttattaatac aaataggtgg tctaggtatc
     7621 gtaaccgtaa ccctattgac actagtattt ttaaatagaa agatatcaat gaaaaataga
     7681 tttttgatta tggttacatg gaatattgac gaacctggtg gtgttattaa gctaattaaa
     7741 cacttggcta tttatagttt agtcactgaa ttaattggta tgatttgttt gtgtttatct
     7801 tttataccaa aatttggtat aggcaaagga ttatttttaa gcttattcac atcagtatca
     7861 gcttttaata atgctggatt tgcccttttt aagaataact taatagatta ttctaatgat
     7921 ccaattgtca ttatcacaat ctcaatactc ataatatttg gaggtattgg acattttgtc
     7981 gtaatagact ttattaattg taaaaaattg agtaaattat ctttacattc taaattagtc
     8041 ttaactacaa ctagtatcct aataattata ggagctatta cattcttttt attagaacag
     8101 tttaatacta tgcaaaatat gggactagtt gaaaaaatcg gaaattcttt tttccaatca
     8161 ataacaacac gaacagcggg ctttaacagt attgatatag caagcattaa caaatctact
     8221 gctttaatgt taatgctact tatgtttatt ggtggtgccc ctctcagtgc agctggagga
     8281 attaaaataa ctacttttgc agtagcgttt atttttgtat taaattatat acgtaaagaa
     8341 aataatgttt cagtattcaa taaagaaata tctgacaaat atataaaact atctattgtt
     8401 accattaata tctcatttct atttatcagt atcattactt ttatattatc gataattaat
     8461 ccgaacatat cattaatcaa gttattattc gaagtggttt ctgcatttgg aacagtcggg
     8521 ttaagtatga accttaccac agaatatcat ggtattacta aaataattat tatattcgtt
     8581 atgctttgcg gtaaagtagg actattaact ttattaagaa catttatacc accaaaaagt
     8641 ccaaaaaatt accgctacac taaaggacaa atttatctat aaaataacta tccatcttta
     8701 acaagaaatg aactagtgtt ttaatctatt acatttaaaa aactacctaa acaggtagtt
     8761 tttttgtttt ttatttattt taggacgaca aaacgtctta tatctttaaa aaagcaaatg
     8821 cataagtaca cctttaaaaa gatctactca aaactaaact atacaatgat ttaaatattt
     8881 aaaacttatt ttttactacg ttaacctcaa ttgttgaaaa gatgaatgaa aatgataatt
     8941 taataatgta tacttttgat tgtttaaaca tgtacatttt ctaaaattct agagataatt
     9001 ttattgctgt gaacaacaat gaaattatct tcagacataa atacggattt tgaagttact
     9061 actctttcat atttacaaaa attaataatt gttatgatga acttaaacat gaaaataagc
     9121 aaaagtgaaa tagctagaca catgggtgtt gatagaagag cagttgataa atattcaaat
     9181 gtctttgacc tttattttat aaattcttcc ataaaaataa ccacacttct aaatcaatag
     9241 gtgggtgtgg ttttgtgctt tatattttaa gaatagccat cttcaaatta ttttcacttg
     9301 tatgatcttt gatttctgta aatccaaatt ttctataaaa ttctcttaat ctatctacat
     9361 cctcatactc tatccaaata tatgaaccgc caaatatgtt cgcaccttct aaaactttca
     9421 catatgctaa agttaataaa tcgtttgcat ttatggattt tgtagccaat gcttcttcag
     9481 aataattttt ttctatttga gcaattaagt atccttgtat gattaaattg tcatttccac
     9541 tgtgagtttc acccttttga tataacttct tctttaacgt gtttgatatt ttatctaaca
     9601 tacgttttgt aaaaaactaa gggtttattg ctaattgtaa aatagccaac tagtatttgt
     9661 ttgtttttga attgtgaaaa aactaaatga gttctagaca ggttttgtct atcaaatgct
     9721 atagacttat ttttcaaaaa taaatcaaca tcgtgtgctg attcacttcc tgaaattgat
     9781 attgtctcaa atgaatttag tatatttaaa actgaaggta tctttcgctt aacctcttgc
     9841 ttatcacctg ttaacaattc tgaaagtgat attactttta cagtcaatta cttcgataat
     9901 ccttcaatag attttcaatc tcacaagttg attttatttt agtcgctttt atgtttgttt
     9961 tgttcacatt actattattt tcgttaatct ccattgcatc tattagtttt tgcgctgatt
    10021 tttttgtgaa tttataatca gtagtaatac ttattgtagc catactactc acttccttat
    10081 tttacttcat ttgacaacca agtatacatt gtctacgttg tcttcttgtt tacaatgtat
    10141 catgaaagca tcttttcgac aatagattca ataaatttct ttagtgtata tgattttaca
    10201 cttaaaccaa tttgtttctt agcataaaaa cacccatgct gtaactagtg tgggtgtttt
    10261 caaaatgcag acagcttctt attacaatac gcaagtgagt cctctgcaaa gccgaattga
    10321 ttaccggaaa tctaattaca ttacaacata ctagaagaaa ctccttatat actcaaaagg
    10381 tatactaaac ttctctatat aatctatatg attttaacat tgaaaaaaac ccccattatg
    10441 aatctaaata acacatcagc acataataaa gacgccttcc aaaagttttg gtaactaatg
    10501 gaaaacgtct taaaaacata ttttcatacg ctctaatatt tatttggaac gaatcatact
    10561 cgtgcataat gtaattttct agttagtcaa aactagaaac tgttttacat cattcctggc
    10621 attaagggca gtatgaaata gatataaatc atatacaaaa atcctattaa atcagcatcg
    10681 tgtattcttt atgcattcaa tttttttagt ttttttattt cattatgacc aatttatgac
    10741 caccaattat cacacacata aatctctata agtatatttg caatgataga tatgattatc
    10801 catataatgc tttttcattg caatataatg cttatctcag catcttatat cacctaataa
    10861 agttagtgta aaatattaaa attgataaaa ctatataaat ttgtccaccc atttttgatt
    10921 aataaataag cgtttgatgc ctgcaacgtg tgattatatt aatattaata cgtataaaca
    10981 tcgtcatatt tttttatatt caacgttgta atcacgtatt atactgaata taaaatatca
    11041 aaaatagata aatacatata tttcccgaaa caacatcata ttaatccaca ttaaacatac
    11101 aaaactcatt ctgcttatta atagttctaa gatatcatgt tataattagt gaataaaatc
    11161 acaaaaggaa ggttttaaat catgagactt caaaaagcac ctttagtaac atcgggattg
    11221 atcttaggtt tgttggggtt gggcaatctc ttgaaagaca tatcacttgt tttaaatgct
    11281 atttgtggta tctttgccct ctttatttgg gttcaccttt tatgtactat tctcaataat
    11341 tttaaaaatg ttaaagaaca attgaatacc cctctagttt catctgtgtt cacaacattt
    11401 ttcatgtctg gatttttggg aaccacttat ttaaatacgt attttagtga tgtaaccatt
    11461 atcactagct taataacacc cttatggctc ttatgtctta tggggattat gatacatatg
    11521 attatttttt caattaaata tttaaaaggg ttctcgctcg aaaatgttta tccatcatgg
    11581 acagtgcttt atattggtat tgctattgct ggattaacag caccaatcag cggattttat
    11641 ttaattggaa aattatcagt tatatatggt tttttagcaa catgtattgt cttaccaata
    11701 gtattcaaac gattaaaaac gtatccattg caaacgtcta ttaaaccgaa cacttccaca
    11761 atttgtgcac cattttcttt agttgctgcg gcatatgtta taacttttcc taaggcaaat
    11821 gatctaatcg ttattattct tttggtattg gctcagtttt tttattttta tattatattt
    11881 aaacttccaa agttgctaaa agagccgttt tcaccagtat tttcagcttt cacttttcct
    11941 ttagtaattt cagcaactgc cttaaaaaat agtttacctg tactaacgta cccagaaatt
    12001 tgggaatggc tcttattttt tgaaattaca ttagcaactt taatagtatt aagagtcttt
    12061 ttaggctatg ttcatttctt ttttaaaact agcccgaagg ctagctataa ctcttaatct
    12121 aatgtagcac ttttaaaaat atcttatagt aatgacaaaa tgataaagag aaatatgaca
    12181 agacatatct taataaatct ttgctcaaaa tcaaccagcc attcagctgc cgattttact
    12241 tgcttcatat aaaactgggt atagggtaaa aaacttaatt ctatacccta ttattttttt
    12301 ctctttatcc accaaccctt aaacttgctt gaatgtttgc attattaaaa ttagctttaa
    12361 actaaagatc tgaggtttca attaccattt ggtaatccaa gacaatgtaa tatttatttg
    12421 taattgtttt agtattttct taactgttag ataactttca attttataaa ttactataca
    12481 cattaaaaat aaaatcatta aaagtactaa tactaatgaa agtcaaacac tttcctcact
    12541 ttatcatttt tcaaccattc cgccatatat taagtatcta taatcaatac cctaaattta
    12601 tacatacacc taatatgttt acataaaaat aggcaagtac cgaagtacct gcctgttatg
    12661 cacatttaaa tcttgagaga aatgttaaaa agttctagta aaataatagc acattttatc
    12721 tttaaatgta aatagaaagc aggtgtgtta cacacctgct taaatagaca tgactatgtc
    12781 atttcaactg atttctcccc attagtcgcc tagtacctga ctagttgggg cagaaccatt
    12841 ccatgttcta ataggcaagt aataacgttg cccctcccgt ttatataata cccataaata
    12901 atatttatat aactaattta tgtaatttta caaggctcaa aattgagttc tgcagttaaa
    12961 ttcataaaca tttagtatgg aattttagtt ttgccgtttg atgttatgtg tactttgatg
    13021 cgtactggta caggtaagct agctatttcc gataaagctc tcagacctaa atgttggtat
    13081 gtaccaacat ctttaatttc ttcagaaact ttaatgaaac gatgagcttg tgttctatct
    13141 aagttgatat ttttcaacca attaccaaat tctccatgtg ctaggtcatt ttgtttcata
    13201 tggttttaat cttcgaccaa tctcaaagat agattgacca gcgatgttta aagccatatt
    13261 tcacgacgtt atgtttacag ttaaagtatt tgtgcgtggt tcccatccct tactgcaaca
    13321 caggacgttt ctcagcgtaa aaaacgccac tagaaagtga ctttaaagaa tataactaat
    13381 tcaaacttat attaattaat attctttaaa tgaccactca cactttgttt tttgctattt
    13441 gtaactttaa aatgttgttt aaatctatat tttttgatat agctccctat gtaacaaaca
    13501 ctttttaatt aatatatatt taaacaataa tttagagatg gttaattgat tcatttaaat
    13561 aatatttata cattctatat gtaaacgttt acacatttga atgaaggaga attaaaaatg
    13621 aataaaaaat tactaatgaa tttttttatc gtaagccctt tgttgcttgc gacaatcgct
    13681 acagatttta cccctgttcc cttattatct aatcaaataa tcaaaactgc aaaagcatct
    13741 acaaacgata atataaagga tttgctagac tggtatagta gtgggtctga cacttttaca
    13801 aatagtgaag ttttagataa ttccttagga tctatgcgta taaaaaacac agatggcagc
    13861 atcagcctta taatttttcc gagtccttat tatagccctg cttttacaaa aggggaaaaa
    13921 gttgacttaa acacaaaaag aactaaaaaa agccaacata ctagcgaagg aacttatatc
    13981 catttccaaa taagtggcgt tacaaatact gaaaaattac ctactccaat agaactacct
    14041 ttaaaagtta aggttcatgg taaagatagc cccttaaagt attggccaaa gttcgataaa
    14101 aaacaattag ctatatcaac tttagacttt gaaattcgtc atcagctaac tcaaatacat
    14161 ggattatatc gttcaagcga taaaacgggt ggttattgga aaataacaat gaatgacgga
    14221 tccacatatc aaagtgattt atctaaaaag tttgaataca atactgaaaa accacctata
    14281 aatattgatg aaataaaaac tatagaagca gaaattaatt aatttaccac tttttctgta
    14341 ataattatta ataaagggag tccgttaatt ataataagcg ttctcccttt tatctttata
    14401 acgctttaat ataaagctag ggtgaattag taaactaatc ctcactaact caatatgata
    14461 atcgttttta ctgtaatcaa tcctctaacg gtatatcatc cacaatcaca gtatgattag
    14521 gattagcgtt agatacctct tgtaccgtct tatctaaatc ttcatcatcg ccatcccatt
    14581 caccaatatt aatgaatata ggaacatccc cgttgatatc atgcttatct gtaaataact
    14641 tatggtattt acccaacata tcacgagctt ttaaacgatc actaggtttt attggtacct
    14701 ctatcagttc aacatgttcg ttatagacta gctgtacttt gccactttgt ggattctctt
    14761 tatattcccc tcgcttgact acaacttctt tcgtttctgt ctcatcaccg actgccgcat
    14821 tcgtaagcac atgtagtaac tcttttgcgg ttaatacatt ctcatctata atcttatctt
    14881 tttgttcttg tatatattgc ttgatgtgtg gcttctttaa taacctacat cctgtcacat
    14941 gtgcactatt tgcgctatag cctgctttta tggcactttg tgttacatta agtgttctta
    15001 tatactcatt cacaaaacgc gcttgttttg ccgttaactc actcattcta tcacctccac
    15061 aattttatct aataaggttt cataccataa tcttacagat tgttctgaac actctaagac
    15121 attgctaata tctttaaaac tacgtccttg tattaaagaa tcgaaaatat aaaactcttt
    15181 gtcattagct acttggtcaa caatcatttc taagtgattc tttacaatat gatcatcaat
    15241 gttatcgtct gccatccatt cattagaatt ttcatcacct attgaaaaga attcatcggt
    15301 atttatttca tcatctatca acacatcact tctagttcgc ttatgataat cacaaacgaa
    15361 gtcttttatt tgctttttat ccattgttac accactttta catatgaaga ttggtgatat
    15421 gcatttactc gtgcaatctt actgttttca attgctgtat ttctttgttt ttgacgttct
    15481 gaacgttgtt taatacttgc ttgatacaaa tcaacttgta agcgttcaat gacgttgtaa
    15541 ggcttatatc gtccatttga acgcatatat tttacaactt gcttctgctc tttttctgta
    15601 taatgattta gtaccttttt caacaacgcc atattattta tagatctatt tttatagttt
    15661 tgtaaccctg cttttgtttc aataattttg ataactaatt tttcaatcgg atatgagaca
    15721 gacacgaccc ccattatttc atcacatgtt gtggtcgacg cactcatatg gtacatactt
    15781 tcaatttgga attcacacat cttaattttt ttattaataa atgctgggtt aaattgcgtt
    15841 aatagttgat actcagatag tttattgtcg ccattacgat aatataaaca attcttcgtt
    15901 ttaagcagtt tcatttattc acccctataa acagagccta cccgaattgg ataggcaatc
    15961 attgctattt aataatcctg ttttgcttag ctaaattttg tagcgttgta ccatattgct
    16021 tttgcttaga ctgttctgat tgttgtaact cacttgaaat ctcctgcata ttgtttttaa
    16081 tatccaaatc aactgcattt attaatagat ttgtatcttc ttcatttaaa ccaaatgcat
    16141 ttgcgacctt tttagtatta tttaactcgt attttgtttc catttaatta ccctttcttt
    16201 ttaacgtttt aaaaacaact tgttattgtg ttcgtatggc aaatcattac cattaatata
    16261 tgatgtaaat atattttctc taaagtagcc attcaatgct tccctagcct ctttatcatc
    16321 atataattgt tcttgactat aaatactcgc atattgctga tgctcatctt catatctatc
    16381 attaatatct tctatttcat caatgatctc attatatgca tcgactacct tttttaattt
    16441 acctaaagct gattgttttt ctgattcata taatgatgac aactcgcttt gatgttttaa
    16501 taattcaatt gtcttttgat atttaacttc tttcgacaca cttttctttg tctctaagcg
    16561 tttattaagt gcttttagtt tcttttcatc agcatctgtt gcttgatata ggttatctgc
    16621 tttatcatct tgtccatcca tgattaactg tttatatgtg gacttatcta tctttatttt
    16681 actctccaat gcattacgct cttgtttcaa ttcttgtata gccttttgtt gatctattac
    16741 aaattggttg tattctttaa agtacgattc agttttcatt tttatcccct ttacacttta
    16801 attcgtttca aagcttcata gcgtttcata ctgccatcag ctaatttctt aatacttctc
    16861 atcgcttgtt gcttttcttg ttctgtcata atgatgtaat aaccacgttc actaggttta
    16921 taactgcacc cgataggata gccataatca tatactaatg aattgattac tcttcgtaac
    16981 catcgttcat tgcttgaatt atattcatat cccaattgat ttaagatttt agttttagta
    17041 atatacttat tggacgtatt ttttatcaca ttgaaaactt gcaggtgttc ggtgggtaaa
    17101 tgatacgtct ctttttctgc gatactttgc atttctacac ctctttcttt taattatttc
    17161 atacctaaat tataccattt ttacaggtct aaaacaaact tacgttcgct ttatagcgcg
    17221 ttttatcaat tgtttagctt atcatatata acacttataa aatcatgtta taaacttaac
    17281 gttaggcttt tcacattaac ctaatataga acttaagttc gataaaataa cacgaacaaa
    17341 cagcgaacaa acttaacttt taggcctatg ccaaaaacac aaactttagc ttgtattagc
    17401 gttaacaaag ttctcacacc ttgcacaaat cttgccattt tttcaattct caaagactgt
    17461 ataccttccg atgttaaaag ctaacacctt tatataacct tattattttc aaagccataa
    17521 aacagctcaa tatcaacatt tcacactttt ttgaatttcg cctacctatc tattaactca
    17581 ctattgttgc aacctttaca ttttaaaact tctatacctt atacttttat attaggagcc
    17641 acacaccacg tgagactcca tatttaatta cttattcaaa ctgtaataag atgacttcaa
    17701 ctcagttaac ttatgctcta aagccttgta atcctcttgt gtcgcattct catcttgtac
    17761 aaactcagtt actaatctca acccctcaac taactctggt gctggttcat tgattcctgt
    17821 agctaactga tacaacattt caatattcgc tatcacatca gtattactcg attgaatgcc
    17881 ctcaagtgta tcggtatcaa atccattttc taggtactca aacacatcac tattatttga
    17941 ttctgcatat gtttgtaa
//
LOCUS       DAMZPP010000051        17849 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMZPP010000051 DAMZPP010000000
VERSION     DAMZPP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17849
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.051"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(179..1615)
                     /locus_tag="POH73_001284"
     CDS             complement(179..1615)
                     /locus_tag="POH73_001284"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDI6887248.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVNYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDFGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGRGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(1631..2422)
                     /locus_tag="POH73_001285"
     CDS             complement(1631..2422)
                     /locus_tag="POH73_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI6887249.1"
                     /translation="MGNKYKDYAQDILTAVGGVENIVNVSYDTVRMTIHMHHAIPSTA
                     NEVTQIDGVASVDENETQLVIVFNEEVKYVYQQLQLLMDDAKHQEDTNHDAVDTQETE
                     QKAQAKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     VSMIVPTKHAVGIQSEDGVDIVIHIGVNTVDLEGKGFECFVKQNDRVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            2569..3273
                     /locus_tag="POH73_001286"
     CDS             2569..3273
                     /locus_tag="POH73_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6887250.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSAGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(3499..5790)
                     /locus_tag="POH73_001287"
     CDS             complement(3499..5790)
                     /locus_tag="POH73_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HDI6887251.1"
                     /translation="MSMQSSYKRLVKVLLTILVIVFSILLVGGWYIFKNEAPRPTKIV
                     DQQGHTLVTKGELISGQAIYEKYGLTDYGSYLGNGSYLGPDYTAEALHHYLIGMRKYY
                     AQDIYHKQLHKLNHAELAVVKDKVMKEIRINRYSEKEDQLVLTPGQVYGLKYLQDYYK
                     KEFVNNPKQVGLNENMIKQFQNDDYMVAGNKVEHLSQFFFWGAWLSSTDRPGKTFSYT
                     NNWPYDVDAGNTLPSAGILWTAISVTLLIAGLAFIIYIQKRYEFDMKPTYESERELPK
                     IEVDSKITDSQRKVGKYLVVVMLLFLVQILLGELLAHYYVENKFFGIEIQRLFPFNIA
                     KTWHVQLVIFWVATTWLAAGIYIVPKVLGKEPKKQGVLVDILFWALIIVVMGSMIGEW
                     GHILGWIDSHWWLFGHFGWEYIELGKFWQILFIVGMVLWVIILCRGFLPAIRNKVQAN
                     HDKRHLLTLLFVGAIAIPLFYLASLFIMPNSHVTFADYWRWWIVHLWVEGIFEAFAVV
                     LIGFLMVNMKLTTIETTIRALYFQLILLLGTGIVGMGHHYYWQGDHSIWLALGSCFSA
                     LEVVPLCLLIWEAYTHYRLYKKSDIAFPYKGTFIFLASTGLWNAIGAGALGFLINTPA
                     VNYFEHGTQWTAAHAHGSMAGVYGMFSIAIALYVFKNITKKEFWTPKVEKWIKISCWA
                     LNVGLAGMVFITLMPVGYIQLKDALEHGYWHSRLTSFYEQPLVKAIMWGRMPWDIIFT
                     VGVIILVVIFIRGYRHLKPKYRV"
     gene            complement(6131..6832)
                     /gene="lrgB"
                     /locus_tag="POH73_001288"
     CDS             complement(6131..6832)
                     /gene="lrgB"
                     /locus_tag="POH73_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HDI6887252.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(6825..7268)
                     /gene="lrgA"
                     /locus_tag="POH73_001289"
     CDS             complement(6825..7268)
                     /gene="lrgA"
                     /locus_tag="POH73_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HDI6887253.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(7381..8121)
                     /locus_tag="POH73_001290"
     CDS             complement(7381..8121)
                     /locus_tag="POH73_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HDI6887254.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNSANAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPAYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(8124..9878)
                     /locus_tag="POH73_001291"
     CDS             complement(8124..9878)
                     /locus_tag="POH73_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI6887255.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRNVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(10129..10803)
                     /gene="scdA"
                     /locus_tag="POH73_001292"
     CDS             complement(10129..10803)
                     /gene="scdA"
                     /locus_tag="POH73_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HDI6887256.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAASLEK
                     KNVDLNELLQRLNEVEQTNTPGSLNPKFLNVSSLIQYIQSAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGQLLVKMSELTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(10943..12667)
                     /gene="tarS"
                     /locus_tag="POH73_001293"
     CDS             complement(10943..12667)
                     /gene="tarS"
                     /locus_tag="POH73_001293"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDI6887257.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFEGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISMKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTA
                     HITYPLNK"
     gene            complement(12700..14388)
                     /locus_tag="POH73_001294"
     CDS             complement(12700..14388)
                     /locus_tag="POH73_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6887258.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(14410..15435)
                     /locus_tag="POH73_001295"
     CDS             complement(14410..15435)
                     /locus_tag="POH73_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDI6887259.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(15428..16144)
                     /locus_tag="POH73_001296"
     CDS             complement(15428..16144)
                     /locus_tag="POH73_001296"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HDI6887260.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            complement(16420..17589)
                     /locus_tag="POH73_001297"
     CDS             complement(16420..17589)
                     /locus_tag="POH73_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6887261.1"
                     /translation="MIKTTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQHAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVQNQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGHASQRICQTIFK"
BASE COUNT         5688 a         3382 c         2560 g         6219 t
ORIGIN      
        1 attgcctgtt gaaattggtg ttccaatttc tctgtgttgg gggcctgcca actcgcacat
       61 tattgtaagc tgtatttccg ccagcttcta tgttgggggc ccgccaatat aacactttag
      121 ggcctaccat attaatttat gtctcgggct ctaaccctaa aatacttata atgtaccttt
      181 ataatttatc tccatttgat gctatcactt gttgatacca atagaacgaa tcttttttca
      241 agcgttccat cgtgccacga ccatcattat ctcgatcaac atatattaaa ccatagcgtt
      301 tcttcatttc accagttgta aatgaaacaa tatcaatgat tccccacggt gtataaccga
      361 ttaaatctac accatcttga tcaactgctt ctatcgctgc tgtaatatgt gcttttaaat
      421 attcaatgcg ataatcatca tgaatatgtc catctaccac ttcatcaact gcaccaaaac
      481 cattttccac aataaaaagt ggcaactgat aacgatcata taacacattc aatgtatatc
      541 ttaagccatc tggatcaatc gcccaacccc aatcactcgt tgcgatatgc ggattctcca
      601 cagaatgatt caaaccaccg ttgacgatgt tgttttcaac tgtagtatca acatcatgtt
      661 ttacagccgt agacatgtaa taactaaagc caatataatc aaccgtgccc tgctgtaaaa
      721 tactgtcgtc cccttcttgc catccaaaat catatccttt tcgtgccaac atttttttag
      781 catagcttgg ataataacct cttacttgaa catccgggaa aaagaaacgt aagcgattcg
      841 caatttgtgc ttccatcata tctttcggat gacacgaata tggataaatg ggtacatgtg
      901 aaatcattgt tccaatctta aatttcggat taatctcttt accaagacga accgctaaag
      961 cactggctaa aagttcatga tgtgctactt gatacaagac ttcttcagga ttatcatttt
     1021 ctgtcaatgc taccccagaa ttcgtccata aaaagatagg atttgatgtg tccatctgat
     1081 tatttatttc attaaacgtc atccaatatg tcactttatc tttatatctt tcaaatacaa
     1141 cacgcgcaaa atgcacaaaa taattgacaa cttctctatt tctaaatcca ccataatgtt
     1201 tcgctaaatg aagtggcatc tcaaaatgtg ataacgtcac aacaggttca ataccttgtg
     1261 caattaattc atcaaagata cgatcataaa aggcaagtcc ttcttcattt ggcacatctt
     1321 catccccatt cggaaagata cgtgtccaag caatcgacgt acgtaaacat ttcaatccca
     1381 tttctttaaa caaggcaata tcttccttat aacgatgata aaaatcaata ccttcatgat
     1441 ttggataata gtgattggga tctatagatt ctgtaatctg acgtgcttta ccatgtgcac
     1501 cactcgtcat aacatcaatt acacttaacc ctttaccacc tttatcatat ccaccttcaa
     1561 attgattggc ggcaagagcg ccaccccaca taaaattttg cggtaatttt gtcattatta
     1621 caacacactc ctagttttta aatattttaa aaataacatc ttcagtcgta atgatttcat
     1681 tcattgtcag ttctactttt cctaaatcgg cagaattgct aataacgaca ataacgtcag
     1741 cattgtagcc ttgttgttgt atatattgct ggtcgaattg taacaacgtt tgccctgctt
     1801 caacacgatc attttgcttt acaaagcact cgaatccttt accttccaag tcaactgtat
     1861 tcacaccaat atgaatgact atgtccacac cgtcttctga ttgaatacca actgcatgct
     1921 tagttggtac aatcatagat actaaaccat tgaacggtgc gattactttg gattcttcat
     1981 gagctttgat tgccaagcct tcgcctacca ttttctctct aaaaattgaa tctcttactt
     2041 ctttaagtaa aatacgacga ccagcgattg gtgctttcac taaaattggt gttgtcactt
     2101 ttgcttgcgc tttctgttct gtttcttgag tatccacagc atcatgattc gtatcttctt
     2161 gatgcttggc gtcgtccatt aacaattgta attgttgata tacatatttt acttcttcat
     2221 taaatacgat aactaactgt gtttcatttt catcaactga cgccacgcca tctatttgtg
     2281 ttacttcatt agcagtagaa ggaattgcat gatgcatatg aatggtcatg cgtaccgtat
     2341 catacgatac attaacaata ttttcgacac cccccacagc tgtaaggatg tcttgagcat
     2401 aatctttata tttattgccc ataagtatta ccccctcaac ttgataatag aatggaaata
     2461 tatgacttat cataattata attgtaccgg ttcaatttgt aaacgccgat acaattataa
     2521 tattttgtgc tataataatt acagacaaag tgaaacgagg acagaatatt gttaaagtat
     2581 gaacatattg ctaagcaact taatgcgttt atacatcaat ctaatttcaa acccggtgat
     2641 aaattgccaa gcgtgacgca attaaaagaa cgttatcaag taagtaagag tactatcatt
     2701 aaagcattag gcttattgga acaagatggt ttgatctatc aagcacaagg cagtggtatt
     2761 tatgtgagaa atattgcaga tgcaaatcgt atcaatgttt ttaagaccaa tggtttttct
     2821 aaaagtttag gcgaacatcg aatgacaagt aaggtacttg tttttaagga gattgcaacg
     2881 ccacctaaat ctgtacaaga tgagctccaa ttaaatgcag atgataccgt ctactattta
     2941 gagcgattaa gattcgtgga cgatgatgtt ttatgtatcg aatattctta ttatcataaa
     3001 gaaatcgtga aatatttaaa tgatgatatt gctaagggct ctatcttcga ttatttagaa
     3061 tcaaacatga aacttcgtat tggtttttca gatattttct ttaatgtaga tcaactcact
     3121 tcaagtgaag cttcattact acaattatct gcaggtgaac catgtttacg ttatcaccaa
     3181 acgttttata caatgactgg caaacctttt gattcatctg acatcgtatt tcattatcgt
     3241 catgcacagt tttatattcc tagtaaaaag taatacaaac atcaaaacgt ctatattcca
     3301 gttataaacc ggagtataga cgttttatag gacataattt ctttaaaaac agtgagaagt
     3361 acattttcaa tcagaaaatt actttttact gttctttata tatacataat ctaagaactt
     3421 atagtttgaa tcctattgta taatatctct tcgtctcaaa cgttaaaaca cactaagatc
     3481 actcagtaat aagatgatct atactctgta ttttggtttt aagtgtctgt atccccttat
     3541 aaaaataact actaatataa tgacacctac tgtgaatata atgtcccaag gcattctacc
     3601 ccacatgatt gcttttacaa gtggttgttc ataaaatgac gttagtcttg aatgccaata
     3661 accatgttct aatgcatctt taagttggat ataacctact ggcattaatg taatgaacac
     3721 catacctgcc aagccaacat ttaaagccca acatgatatt ttaatccatt tttcaacttt
     3781 cggtgtccaa aattcttttt tagtaatatt tttaaaaaca tacaacgcaa ttgcaattga
     3841 gaacatacca tacacgccag ccatagaacc atgggcatgt gctgcagtcc attgcgtacc
     3901 atgttcaaag tagttaactg ctggtgtgtt aattaaaaag cctaatgctc cagcaccaat
     3961 tgcattccac aatcctgttg aagcaagaaa aataaatgta cctttgtaag gaaaagcgat
     4021 gtcacttttc ttatataagc gatagtgtgt gtatgcttcc caaattaata aacaaagtgg
     4081 cacgacttct aatgctgaaa aacaagaacc cagtgctagc catatggaat gatcaccttg
     4141 ccaatagtaa tgatgaccca taccaacaat acctgtacct agtaatagga ttaattgaaa
     4201 atataatgca cgaatcgtcg tttcaatggt agttaatttc atattaacca ttaagaaacc
     4261 aatcaacact acggcaaatg cttcaaatat accttcaacc cataagtgaa caatccacca
     4321 tcgccagtaa tctgcaaacg tcacatgtga attcggcatt ataaataatg atgcaagata
     4381 aaataacgga attgcaattg ctccaacaaa caatagcgtc aataagtgac gtttatcatg
     4441 attcgcttgt actttgttgc gaatagctgg taaaaaccct cgacaaagaa tgataaccca
     4501 taagaccata cctactataa ataatatttg ccaaaattta cctaattcta tatattccca
     4561 accaaagtgc ccgaatagcc accaatgaga atcaatccaa cctaaaatat gaccccattc
     4621 tccaatcatg ctgcccatca caactatgat taaggcccaa aacaagatat ctactaatac
     4681 gccttgtttc tttggttctt tgcccaatac tttaggtaca atgtatattc ctgctgctaa
     4741 ccaagtcgta gcaacccaga aaataactaa ttgcacatgc catgtcttcg caatattaaa
     4801 tggaaagagc ctttgtattt ctataccaaa aaatttattc tctacataat aatgtgccag
     4861 taattcgcct aataatattt gaaccaaaaa tagtaacatg acaacgacta aatatttccc
     4921 aacttttcgc tgactatcag tgattttact atctacttca atctttggta gttcccgttc
     4981 cgactcatac gtcggcttca tgtcaaattc ataacgcttt tgaatatata taatgaatgc
     5041 caagcctgca atcagtaatg ttacagatat agcagtccat aaaattcctg cactaggcaa
     5101 cgtatttcct gcatccacat cgtatggcca attatttgta tatgaaaatg tttttccagg
     5161 tctatctgtt gatgatagcc aagcacccca gaaaaagaat tgacttaaat gttcaacttt
     5221 atttcctgcg accatataat catcattttg aaactgttta atcatatttt catttaaccc
     5281 tacttgttta gggttattaa caaactcttt tttataataa tcttgcaaat attttagacc
     5341 gtatacttgt cctggcgtta atacaagttg atcttctttt tcagaataac gattaatccg
     5401 tatttctttc atcactttat ctttaactac ggctaactct gcatgattta atttatgaag
     5461 ttgcttatga taaatatctt gtgcataata tttacgcata ccaatcaggt aatgatgtaa
     5521 tgcctctgct gtataatctg ggcctaagta ggaaccattg cccaggtagg agccataatc
     5581 tgttaaccca tatttctcat atatcgcttg cccactgatt agctcgcctt ttgtaacaag
     5641 cgtatgacct tgttgatcta ctatctttgt tggacgaggt gcttcatttt taaaaatata
     5701 ccaaccacct actagtaaaa ttgaaaacac aatcactaaa atcgttagta gcactttaac
     5761 taaacgttta tatgaacttt gcatactcat ttataaaatc tcctcttaat tgcattctta
     5821 catataagca tacgccgttt ttttataaag tgttgatgac attaagtaat ttcaagaatt
     5881 tgtcgaaaaa tatttaattg aaatttcaca aatgatataa cttacatttt aattcttagt
     5941 taaattaatt cgttctatca tttttcaaaa gccgcaatac ataaaacgaa cgcctaaata
     6001 ccataaaacc tagtaaaaag taataaatac ataaaaacgt ctatatccca gttataaacc
     6061 ggagtataga cgttttttta cgataataac aatggctcaa attgctatta tcttgcttag
     6121 gtttttcgtt ttagaagaat attgctacaa agacaggcac aactgctaca acaactacac
     6181 caactaacac taaagctata cttgccattg attcttctac aggtcctaat tctttggctg
     6241 gtgctacacc taatgtgtga ccacttgttc caagtgctaa tcctcgggca atagggttag
     6301 taattcggaa aagcttcaag aatttattac ctagggcata aataatgaca ccatttaaaa
     6361 taactgctaa tgatgttaat tcttttatac caccgatacc agctgatact ggtaacgcaa
     6421 tcgctgtagt tgctgcttga ggtaacattg ataaaataac atcattggca aattgtgcta
     6481 acttcgcaaa agttaaaata attaataacg ctacaactgt accgatacca atacctccga
     6541 tgatacgatg ccaatgttta acaagcactt cacgcttttt atataacgga atcgcaaaac
     6601 agattgttgc tggttctaag aagaagtaaa taatgtctcc acctattttg taagttttat
     6661 acggaattcc tgttaaatag aggaaggcca caccaaatac catactgaca aatagcggtg
     6721 cgaataagaa gaaacgattt gttttttcaa ataatatggt tgctaagaaa aatggtataa
     6781 cggataacag tattccgaag taaggtgtgt ttagtgctaa gtggttaatc atgagcttgt
     6841 gcctcctcta ttttgatctt ttttgtgact ttgtcacctt tagatctcga agtaactttc
     6901 ataataattt gtgtgacata gccagtacaa ataagtaata gtattgttga gacgattatt
     6961 agtccaatga ttaaaaatgg tgcttggcta atgacaccta aagagttaac aactgagata
     7021 ccggctggta cgaagagtaa gccaatgtta tttgttagtg tcgttcctac tttttcgact
     7081 tcgcctaact taacagcacc agtacataat aatacaaata ataatactaa accgattact
     7141 gatgcaggca taggaattgg cataaatgat tcaattattt tcgatacaaa gagtactaaa
     7201 gcaattacaa tgacttggtg aaaaaagtgt gctggttttg atgcgtcttt ttgttgtttc
     7261 acgaccattg cctcctacgt ttgatttaac taaagtatag atggctcact ttgatttgcg
     7321 tgaattttag tccgaaatac aaaatatcat aggtaaaatg cataaaaaaa aggattactg
     7381 ttaaagtaat cctatcgacg ctttaaaatc tttcataaat gaacgtccaa cttgcatctt
     7441 gacaccattt gtcaatatta ccatataagt gtagttaaac cattgttgca cttctttaat
     7501 gtgtttcgtg ttaataatat atgaacgatg aatacgtatg aaataagcgg gattcaatcg
     7561 tttttcataa cgattcaatg gctctgttgt ttcgtattta tgattcgttg tatgtatggt
     7621 tgtaatacca ttatgtgtgc caatcccaat aatattttgt tgctttaaca tgtgaatttt
     7681 atcgtcaatt tcaacaggta aactttgatc aaaattcgcc gacatatcat tcgcaattgc
     7741 atttgcgcta ttatcatctt tggctttagt cgcacgaact ttattgactg cttgttcaat
     7801 acgtttttga ccaaacggtt tcaaaatata gtctgtcgca tttaattcaa atgcctgtac
     7861 tgcgtattgg tcatgtgcag ttgcaaaaat aatcgcaggt ggctctttca tcttttgaat
     7921 tttagctcct aattcgatac cattttcatc cattaaattg acatctaaaa atataatgtc
     7981 atattgattt atcagtagtg cttccaatgt ttcttttaca ttttctgcct cattaatttc
     8041 ttcaaaacca ccaatttcat ttaataaata tgttaattca ttacgtgcta atggctcatc
     8101 atctatgatt aatgctttca tatttattcc tcctcttgtc tttcataagg aagtacacac
     8161 caaaaagtgg taccgctcga tgtcgattca aattgtaatg ctgcggattt tccaaataat
     8221 ccttttaggc gtaagtttaa attttctaaa gcactaccag ttccagattc tgattctaca
     8281 gatgtttctc ccaacaaatg cattttatct ttagaaatac cctgaccatt atcttgtaca
     8341 ataatacgta catgtgttgc agtttcttta atcactgaca cgtcaatatc gttaccttgc
     8401 tttcgattcg taaacgcatg tttgatagca ttttcaacta aaatttgaat taaaaatggt
     8461 ggtacaagca cattgcgata tttgtcttca acattaatat tgatattaaa tcttcctgga
     8521 taacgtgctt gttcgagtga taagtatgca cgcacttgac ttaactcttt atctaaagta
     8581 atcgtatgtt gctttgagcc ttgtaaattc gctctgaaaa aataactcaa ttctaatagt
     8641 aactctcgtg ccttttcgct atttattcta actaaagctg aaatcgtgtt aattgaattg
     8701 aagaaaaaat gtggactcac ttgtgcctgt aatgacttaa tctcagcatc tttcaataac
     8761 ttactttgcg tttcggcttc accaagttca atttggctac taaaaatatt tgccaatcct
     8821 tctgcaagtt gacgttccac aaaagttaaa tcattagggt ttgtaaaata catcttcaat
     8881 gtaccgacga tagaaccatg catctcaagt ggtatcacga tagctgctct aagcgggcaa
     8941 ttcggatgac tacaaccaat ctcttcttta gtatgaactt ctttcaactt ccctgatttc
     9001 aatacatctt tagacagact tgttaatatt tcatttgttg gtatgtgatg atcactacct
     9061 gcacctacat gcgataaaat ttcatttttg cttgtaattg ctacggcaga tactttcatt
     9121 aaatttttaa taatcatcgc aatttgctgt gccgattctc tattcaatcc ttctttaaaa
     9181 tacggcaatg tctggttcat caattgcagt acatcatgtg tttgaacagc cttcatttgc
     9241 tcctcttgct ttaatgttga aatgataata gacataaaaa tcgccgtacc aacgctatta
     9301 acaataatca ttggtagtgc aattaatgat atgaggtcaa ccgcatatgc tttgtcgtgg
     9361 gaaaatgtta aaatgctcaa catttgaatc atttccataa caattccaat catggcactt
     9421 tttgcaatac tcgggtaacg cttgcgtctt tgagcttgta agccaaaata accagcaatt
     9481 ataccgataa atatagatga tataagataa acttgtgcat ccgccccacc catatacact
     9541 ctgaaaatac ctgaaataac gccaacaaat agacctacaa aagggccacc aactaatcct
     9601 gcgacaccta tcgttaatac acgtgtgtta gctaaagata catcatcatc taaacggaag
     9661 tacacacttc ctgacaaact atgttgatga tcgatgacga taccagttaa attagacatt
     9721 aaggcaaaca aactgaaaat aatacataat tgccaacgtg ctttccatgt acgtcgacga
     9781 ttcattaagt ttttaaaata tggaatattc atcaacacat aggccaaaat aataattaaa
     9841 cctacacgct caagtaataa cattgttagc gatagcacga tagcacctca gtgaattata
     9901 attatgttta ttttatcata atagtgcatg taaaattcat gaataaatta ttaaaaaatc
     9961 acgctacgat ttaattatct acagcattaa aatttcatat catttcttat actatattga
    10021 tcatttttct tttcaaataa tgatgttcat gtcactatta tgacttttat acatagcgaa
    10081 aaactgctgg acttattttt gccaacagtt tctaatcgcg ttatttagtt atgatacttt
    10141 tttaaataat acgtgattct ctaaatgtac gtgttcatgt gttaacactt caagtgcttt
    10201 taatctctga taaacgagtc gccaagtacc acacgcttct atcggaggtt cataactaga
    10261 tgttaattcg ctcattttta ctaacaattg tcccgttgca atgtggtctg aaactaaatc
    10321 atctatcaca gtattaatat cgtctactac ctcaccttgc tcatatttaa tgagttttgg
    10381 aaaatcgaca tcgtcttctt tttgcatatg ctctaacatg ccatttttaa atgtatcgta
    10441 tgtttctttt aactcaacta aatatggatg gttaggtcca tgtactttcg ataacttcgt
    10501 cacataaggt gttaaatttt taaattcttc tcttagaggt tcatgatatg ctgattgaat
    10561 atattgaata agtgatgaaa catttaaaaa tttaggatta agcgaacctg gtgtattcgt
    10621 ttgttcaact tcattgagac gctgtaataa ttcgttcaaa tctacatttt tcttttctaa
    10681 ggatgctgct tctatactta cttgtccgcc gcaacaaaaa tctattccca cacttctaaa
    10741 aatatccgct gctttcggat aatcagttac tacatctgct actatgtcat ttttatttat
    10801 cattatagat aacctcctca tttccagtgt atacaattta atgtatttga aaatgataaa
    10861 cgaggaaatg tcataatcat gtcataaaca gtaaaataaa accctcaaat catgttagtt
    10921 cacaatgatt tgaggggttt tattatttat ttagtggata agtgatatgt gcagtattta
    10981 acttaacaat atttttgaag tctcgcttct taatatcttt gctaagtaag atttgaactt
    11041 cgttttctga tgtttggctt aacattccca tcaccatgtc gtcttttagc acgacactag
    11101 ccataccttc ttcaaataca taaccacgat ccagaccagt taaaatgatt ttgtttttac
    11161 cttttaactt cattgagatt gaatccgtta atgtaatagc tgtaaagtat aacgaaatgt
    11221 tattaaagtc ttttgtgaaa tacggtgtta atctataaaa atcattattc aaatgaatta
    11281 atgcacttgt ttctgcagta taagcatatt tattacgttg attaccaaca cgcacttcaa
    11341 atacttccgt acctatttgg acttctaaga aaacatccca aattttttct ttaggtaaat
    11401 acgctttaat atcttctaac ataatgttaa aacgatattg tttattcgca ttgacactta
    11461 aaggtacata cattgtgtgc ttcttgtttc gtgaatgtaa tttaagtctg acatcataat
    11521 gtccttcgcc aatcataaat tcatttagcg ttagctctgt aacaatttta tgattatcaa
    11581 atttaaatgc tgtcattttc acttttggct tagcgatatc aatgccttca aagtatggtt
    11641 cgccttcatt gaattgaatg tttaatttac catctacaat atcaaaacgg tagtattgac
    11701 cttgacggta actttcttct acagttctat agttgtctat atctttcgct ctcgcgtagt
    11761 gcaacaatgg tcgtaattta ctcatcacca atgcatctac acgttctgga actgcttgta
    11821 taaagtctcc tagagcatta atccattgtg gttgatcttc tagtttaact ttaagtgaga
    11881 agccattcgt acgagaaaaa ctaaaatgac gatttaaaaa ttctgctaag atttgatctt
    11941 tatgggcttc ttctaaatct gcatttaata tttctacagc aatcaatctc atgacttcgt
    12001 aaaagtcttc aggtgaaaca tacgcactac tcatatgttc accttcacgc tttgtagcat
    12061 aataatacgc tttatcactt aacacactga ttcgatttgc attcaaatat gctttcattg
    12121 taaataattg atcttctgca ctctttaatt cttctggaaa ataaatgtca ttatctttta
    12181 gtaatgctgt tctatagatt ttagtcgggc ttaaagtata gataattctt gaatttaaca
    12241 aagtaacttc aggtgctgtt tctttaaaca tagattgtgg tacaccacga ccattaacac
    12301 ctttcatttt aatcaataag acatctgagt gatgttcatc aataaatgct gctgcatcct
    12361 ttaaagtctc tttgtttata tagtcatcgg agtccacaaa taatacaaat tcaccttctg
    12421 cttgttttaa cgccacattt ctaggtttac ctggaccacc agaatttgtt tcgagttgac
    12481 ttactttcaa gttcaattta ttgcgatact tttcaactat ttgtaacgtt tgatctgttg
    12541 aacagtcatc aactacaacc acttcaaatt cagttttcgg aaaatcttgt ttcgcaaggc
    12601 tattgagtaa ttctgttata tacttttctg aattgtatgt tggaactatt actgaaaatt
    12661 tcatcattat acctctccca ctttgactac tatataaact tagctaccaa ataaatttct
    12721 gactaaacgc tcacttgatc gaccatcttg atatttaaaa tgtttatcta agaatggaat
    12781 gactttttct ccttcataat cttcattgtc caaggcgtcc attaatgcgt caaatgattg
    12841 cacaatttta cctggaacaa atgattcata tggctcataa aaatcacgcg tcgtaatata
    12901 atcttctaaa tcaaatgcat agaaaatcat tggcttttta aatactgcat attcatatat
    12961 taaagatgaa tagtcactaa ttaataaatc tgttatgaac agtatatcat taacttctct
    13021 aaagtcagaa acgtcaacaa aatattgttt atgtttgtct gcaatattaa gtctattttt
    13081 cacaaatgga tgcattttaa ataatacaac cgcgttattt ttttcgcaat atcttgctaa
    13141 acgttcgaaa tcaattttga aaaatgggta atgtgctgta ccatgaccac tacctctaaa
    13201 tgttggtgcg aaaagaatga ctttcttacc tttaataatt ggtaattcat cttccatctc
    13261 ttgtttgatc tgtgtcgcat aagcttcatc aaatagtaca tcagtacgtg gaacacctgt
    13321 aggcactaca tttttctctt taataccaaa tgcttcagcg tagaatggaa tatcggtttc
    13381 agatgataca taagcttttg tatagctacg atgatttaat gaatcaataa atggtccacc
    13441 ctttttacca gtacgactaa agccaactgt tttaaaggca ccaacggcat gccatacttg
    13501 aataacttct tgagaacgtc taaaacgcac tgtataaatc aatgggtgaa agtcatcaac
    13561 aaagatgtag tctgccttcc caagtaaata tggcaatcta aacttgtcga tgatgccacg
    13621 tctatctgta atattcgctt taaaaacagt gtgaatatca tactttttat ctaaattttg
    13681 acgtaacatt tcgttataga tgtattcaaa gtttccagac atcgttggtc tagagtctga
    13741 tgtgaacaac accgtattcc cttttttcaa gtggaaaaat ttcgtcgtat taaatatcgc
    13801 tttaaaaata aattgtcttg tattaaatga ttgtttgcgg aaatacttac gtaattcttt
    13861 atatttacga acgatataaa tacttttaac ttccggagtc gttacaacaa catcaaggac
    13921 aaattcatta acatcgctag aaatttcagg tgtaacagta taaaccgttt tctttgaaat
    13981 gccgcctttt ctaaattctt ttaggtaagt ctgcaataag aaattgattt taccattttg
    14041 tgtttctaat tcgttgtatt cttcttcttg ttctggcttt agattttgat atgcatcatt
    14101 aatcacatct gggtttaact gtgcaatata atcaagttct tgctcattca ctaataagta
    14161 cttatcttca ggtaagtaat aaccattatc taagatagct acattgaaac gacaaacgaa
    14221 ttgattccca tctattttga catcattcgc tttcattgta cgtgtctcag ttaaatttct
    14281 taatacaaaa ttactatctt ctaaatctag gttttcacta tgtccttcaa cgaataattg
    14341 aacacgctcc caatagattt tatctatata tatcttactt ttaaccaacg ttaattcatc
    14401 cttttctatt tacataatcc attttaatac tgttttaccc caagatgtag acaggtctgc
    14461 ttcaaaagct tctgtaagat cattaattgt tgcaatttca aattcttgac cttttaacaa
    14521 cgctaattta tctacaatat ctgggtattg aatgtataag tctacaacat cttggaaatc
    14581 ttttgaacca cttcgactac taccaatcaa cgttagtcct ttttccaata cgagacgtgt
    14641 attaacttct actgggaact cacttacacc taacagtgca atgcttcctt ctggtgaaat
    14701 ataatcaatc atttgattta ttgctgattg gctaccacgc ccaccaacgc actcaaatgc
    14761 atgatcaaat gttaagcctt caggtatttt attaataaag aagacatcat caacaaatga
    14821 gaagtgactc aatttataat ctgttttacc aaagacatat attttagact ctggatataa
    14881 tttacgtaat aaaatggctg taatgtaacc taagttacca tcaccccaaa taccaaatgt
    14941 atttttattt gaaatagatt tcttttcaaa acgacgaata gcatgcaaac ttactgttac
    15001 aagctctgta tatgaaataa tacttaaatc aatatcatca ggtagtggta cagctctatc
    15061 atgatttagc aacacaaaat cttgcataaa cccatcatgt ccacttgatc tgaagtagct
    15121 cgattttaaa tagttttcag caatgacatc gtctttttct gtcggtgtat tcggtaccat
    15181 aactactttt gtacctttat taaacacacc tttactgtca aatacgacct caccaacacc
    15241 ttcatgaatt aaagacattg gcaatttctg agataagaca ttctcatcac ggctaccagt
    15301 ataatatctt tgatcggcag cacaaattga catatataaa ggacgtacaa tgacatagtc
    15361 actgtaaata tctacgttgt tatacgtaac ttcaaattgt ctaggtgcaa ctaattgata
    15421 tacttgatta atcatcggca ataccacctc gaataatagc attcgctact tttaaatcgt
    15481 aaggtgttgt tactttaatg ttatataact caccttttac aagtcgaacc ggtttgtttg
    15541 tttctacaat aatcttacaa gcatcagata aaatactctt ttgctcatca ctcaactgtg
    15601 catagctttc ttttaataaa ttaatattaa acgattgagg tgtttgacct tggtacattt
    15661 cattacgcac tggaattgca tcaatcgttt gattatcttt agatgtaaca atcgtatcta
    15721 tagcatcaat cactgtatct actgcaccgt attctaaagc agcttgaata ttttctttaa
    15781 taatacgatg cgttaaaaat ggtctaactg catcatgtgt cacaatgaca tcgtcatcgt
    15841 taataccatt tgttgattca atatgtttaa cgatattcat aattgtatcg ttacgatcgc
    15901 taccaccttg aatgacttca attctttcat cagaaatttt gaattttcta agtgtatctt
    15961 tcgtatgcgt catccattgt tgtggcgtcg cgataataat tttttcaaaa tcattaatta
    16021 aaataaattt ttctaatgta tggattaaaa tcggtttgtt gtctaaatct aaaaattgtt
    16081 taggtaaagg tacgttaccc attcttgagc ctatacctcc agctagaata ccagcgtatt
    16141 tcataaaata cttcctccat tcaactatat ctatatttaa ttatttaaat ttcgttgcat
    16201 ttttcaattg aaaactcatt ttaaaatcaa aactctaaat gtctgtgtat tacttaaaat
    16261 tatacatatt ttgcttatat tttagcatat tttgtttaaa actatattac attatatcag
    16321 acgttttcat acacaaataa taacatacaa gcaaacattt cgtttattat ttatatcact
    16381 taactaatta atttataatt ttttattgtt tttaagttat cacttaaaaa tcgtttggca
    16441 aattcgttgt gacgcgtgtc catcttctaa tgaacagaat ttttgataaa ataccgttcg
    16501 tgcttcaata tactcatttg cagtctcatc gatttgtttt aatgcatcaa tgagtgctgt
    16561 ttgattttga acaattggac ctggcaactc ttttttataa tccatgtaaa aacctctaag
    16621 ctcatcgcca tatttatcta agtcatacgc atagaaaatt tgcggacgct ttaatacacc
    16681 gaagtcgaac atgacagatg agtagtcggt aactaacgca tcgctgatta agtataaatc
    16741 cgaaatgtct tcataatctg aaacgtcttt cacaaaatca tcatgttcat caatacgtgt
    16801 cacaactaaa taatgcatgc gtaataaaat gacataatca tcatccagcg cttgacgcaa
    16861 agcttctata tcaaagttaa cattaaattg atatgaacct tctcgaatcg cttcatcgtc
    16921 acgccaagtt ggcgcataca taatcacttt tttatctaat ggaatattta atcttgtctt
    16981 aataccgtta atatattcag tatcattgcg cttatgtgat aatttatcat ttcttggata
    17041 acctgtttcc aaaatcttat ctcgactaac atgaaatgca tgttgaaata tcgatgtaga
    17101 atatggatta ggtgacacta gataatccca acgttggctt tcttttttaa agccatcttg
    17161 gtaattttga gtatttgttc ctagcatttt aacattacta atatccaaac caatcttttt
    17221 caatggcgtg ccatgccatg tttgtaagta cgtcgttcgc ggtgatttgt ataaccaatc
    17281 tggtgtacgt gtgttaatca tccacgcttt cgctcttggc atcgctaaaa accatttcat
    17341 tgaaaacttt gtaacatatg gtacattgtg ctgttggaat atgtgttcat atcctttttt
    17401 tacaccccat attaattggg catcgctatg ttcagttaag tattcatata atgctttggg
    17461 attgtcgctg tattgtttac catgaaagct ttcaaaataa attagattct tgtttggcaa
    17521 ttttgatagt aatttaaaag tcgtatatat actgtgttct atcaattttt taattgtagt
    17581 tttaatcatg tcgtacctcc gacgtgtttt tgtaattata ttaatatgta tgagcaagct
    17641 cattgtaacc atgcctatta tagcatttca gcataaaata catttaacca ttacacttgt
    17701 cgttaattat catacgaaat acatgattaa tgtaccactt taacataaca aaaaatcgtt
    17761 atccattcat aacgtatgtg ttttcacatt tatgaattag ataacgattg gattgattat
    17821 tttattttac aaaatgacaa ttcagttgg
//
LOCUS       DAMZPP010000052        17369 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMZPP010000052 DAMZPP010000000
VERSION     DAMZPP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17369
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.052"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(120..1457)
                     /locus_tag="POH73_001298"
     CDS             complement(120..1457)
                     /locus_tag="POH73_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDI6887262.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIIIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            complement(1623..1736)
                     /locus_tag="POH73_001299"
     CDS             complement(1623..1736)
                     /locus_tag="POH73_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887263.1"
                     /translation="MGIVPEVVMWRDVGDMDGVVCGEMLAQVYSVEPEILM"
     gene            complement(1914..2648)
                     /locus_tag="POH73_001300"
     CDS             complement(1914..2648)
                     /locus_tag="POH73_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDI6887264.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFITYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVVKIPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENIIRFFNALT"
     gene            complement(2685..3359)
                     /locus_tag="POH73_001301"
     CDS             complement(2685..3359)
                     /locus_tag="POH73_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDI6887265.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISVA
                     AKTMTKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            3397..3813
                     /locus_tag="POH73_001302"
     CDS             3397..3813
                     /locus_tag="POH73_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887266.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVGRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            complement(4042..4404)
                     /locus_tag="POH73_001303"
     CDS             complement(4042..4404)
                     /locus_tag="POH73_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDI6887267.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(4566..7271)
                     /locus_tag="POH73_001304"
     CDS             complement(4566..7271)
                     /locus_tag="POH73_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HDI6887268.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPKMTIESVLNHFSIAQEDYQAYVEGHLLALPGWAGILYYRSQQHHFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVTKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVTENDNANQS
                     HSVSLNATQAVDENNSELNQVGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRVSLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQAGVYIPETTVFAAAEHHTSTDTLAWVYVPDT
                     LSALALDAYESLNDVMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGKLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVVQAPDFVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            complement(7284..8768)
                     /locus_tag="POH73_001305"
     CDS             complement(7284..8768)
                     /locus_tag="POH73_001305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HDI6887269.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVSIYIATGEWRVPQHIAHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILAWSLLVSWNQMVAF
                     SKGRMARLVGMILISIVTFIYIITHNYFYDVLQNITTHATTPPTISVIISVVILIFGS
                     LLSIWVARHRYSKTFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            9287..9352
                     /locus_tag="POH73_001306"
     CDS             9287..9352
                     /locus_tag="POH73_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HDI6887270.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            9384..9449
                     /locus_tag="POH73_001307"
     CDS             9384..9449
                     /locus_tag="POH73_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HDI6887271.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            9501..9569
                     /locus_tag="POH73_001308"
     CDS             9501..9569
                     /locus_tag="POH73_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HDI6887272.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNY"
     gene            9633..9695
                     /locus_tag="POH73_001309"
     CDS             9633..9695
                     /locus_tag="POH73_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HDI6887273.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(10354..11556)
                     /locus_tag="POH73_001310"
     CDS             complement(10354..11556)
                     /locus_tag="POH73_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDI6887274.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKVLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTDLDEGAITKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            11675..11935
                     /locus_tag="POH73_001311"
     CDS             11675..11935
                     /locus_tag="POH73_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887275.1"
                     /translation="MSTLLYNRDKDISHERESRCINMLFVILVLYIIGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            complement(12224..12538)
                     /locus_tag="POH73_001312"
     CDS             complement(12224..12538)
                     /locus_tag="POH73_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887276.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMNAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(12557..12880)
                     /locus_tag="POH73_001313"
     CDS             complement(12557..12880)
                     /locus_tag="POH73_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887277.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            complement(12877..14184)
                     /locus_tag="POH73_001314"
     CDS             complement(12877..14184)
                     /locus_tag="POH73_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887278.1"
                     /translation="MRCWQIKKDTEPTSAISFMAYEIENANNHNLSKMAIKEQLDRSA
                     ERGMFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNIQGEAFKKLKEGEFS
                     RQNVTNALETVKDGYADIKILYSPASDQNYRYANTQEFINKIKSKYDIDFITGHSLGG
                     RDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITRFVAENDA
                     LTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLKGYAEENNKSF
                     VKHSNNAISKLASIELLRANMMTANGGGLSSSQQKVLDSLTALTIAQSFSQLIDDEIN
                     QIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNEVLNALDEGQVNESSMVREPEQMI
                     SAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            complement(14197..14985)
                     /locus_tag="POH73_001315"
     CDS             complement(14197..14985)
                     /locus_tag="POH73_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6887279.1"
                     /translation="MSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEEQIKKSFAKTL
                     DMYPIKNLEDLYDKEGYRDGEFEKGDKGMWVLYSSIVSEFKGESLKSRGMILKLDRNK
                     RTAKGSYIIRELKEDKNHDVQKNEKKYPVKLVNNKIIPTEDVKNEDLKREIENFKLFS
                     QYGEFKSLNTDRITNISYNPNAPNYSAEYKINDDDNNIKQLKNRFNIKSNKNPKLLFK
                     GAGNIKGSSVGYKEIQIIFNRNKEESVSCIDSIEFKPSEGDYNE"
     gene            complement(15183..15977)
                     /locus_tag="POH73_001316"
                     /pseudo
     CDS             complement(15183..15977)
                     /locus_tag="POH73_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            complement(15996..16796)
                     /locus_tag="POH73_001317"
     CDS             complement(15996..16796)
                     /locus_tag="POH73_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6887280.1"
                     /translation="MRYLNRVVLYIIVMVLSVFIIGCDKSSDTSEKPKEDSKEAQIKK
                     SFEKTLDMYPIKNLEDLYDKEGSRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNEGMVL
                     YLDRNTRTAKGHYFVKTFYEKDKFPDRKKYKVEMKNNKIILLDKVEDPKLKKRIENFK
                     FFGQYANLKELKNYNNGDVSINENVPSYDAKFKMSNKDENVKQLRSRYNIPTDKAPVL
                     KMHIDGNLKGSSVGYKKLEIDFSKGEKSDLSVIDSLNFQPAKVNEDDE"
     gene            complement(16828..>17369)
                     /gene="lpl5"
                     /locus_tag="POH73_001318"
     CDS             complement(16828..>17369)
                     /gene="lpl5"
                     /locus_tag="POH73_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HDI6887281.1"
                     /translation="IYTDFAKSNKSDELDDEGMVLNLDRNTRTAKGYYFVKKFYEKDK
                     FSDRKNYKVEMKNNKIILLDKVNDPNLKERIENFKFFGQYANFKDLENYNNGDVSINW
                     NVPSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHIDGDLKGSSVGYKRLEIDFS
                     KEDRDISVIDYLSYKPAKK"
BASE COUNT         5426 a         3396 c         2435 g         6112 t
ORIGIN      
        1 ttttgttatt ttgcaattat ctaagtttcg attaattcag aaccagtaca aaattttcaa
       61 ttccaaacaa aaaaacacct gagcaacaca aatacttgtg tgtcagatgc ttctatatat
      121 taactaaata attgcacgat aaagactaaa ataataacga caggcatcgc atacttaatt
      181 aagtaatacc aaccactgaa taatctaaat cgatctttac caaaatattg ttgtaataac
      241 tttttatcta ataattgtcc tacgacaagc gtagtaccta atgcgcctaa tggcatcaat
      301 acattcgaaa cgatgaaatc catattatca aaaatcgttc ccgcaccgaa tcttacatct
      361 tttaagatac caaaagataa ggttgctgga atactaatga taaatactaa tatactaccg
      421 atcactgcga cttttttacg ttttgtattg tcattcttcg tgaagttaga aacatttaat
      481 tctaataaag aaatagatga cgttaaagcc gcaaataaga acagcactaa gaatcccaaa
      541 tagaataatg tgcctagatg catttgacta aagaccattg gcagtacttt aaataataat
      601 ccaggccctt cttgtggctc atagccaaaa ctatgtaacg ccggaaatat agctaggcct
      661 gccaatacag atacaaagat attcataaca acgatagaaa tagctgatga cttaatcgtc
      721 atgtctttag aggcataact cgcataagta atcatacctg tagttcctaa tgataacgta
      781 aagaatgatt gacctagcgc aaacaagatt ccatcagcag taatctctga cactcttggt
      841 tgtaaaataa atttcacacc ttctaaggca ccatctaatg ttaaagactt aatcacaatg
      901 atgattaaaa agacaaacag caacggcatc ataactttcg atgccttttc taatcccttt
      961 tcaacaccta acatgacaat aatcatcgta gcgaatatga atataccttg ccctagaacg
     1021 gttaaccaag gatttgatat taccgcttca aaattcattt cttggagatg attgatgcgt
     1081 tgaaatataa ctaattgcca taatacttgt ccgatgtaaa tgacaatcca accaccaata
     1141 acactataga agccaaataa aataaacact gccaaattac cgttccagcc aatgatattg
     1201 agccattttt taccagttaa tttactatat atttgtgttg tatatgtccg tcccattttc
     1261 ccaacagtga attccataat gagtaatggt aatccaacaa aaatggtgaa tattaagaac
     1321 atagctagaa aggcaccgcc gccatagatc cctgccatat atgggaattt ccacatggca
     1381 ccaagaccga ttgcagaacc cgcactagct aaaataaatc cagttgatga cttccattgt
     1441 gattgttgtc ttttcatcat tcacatctcc tttaaagcgt taaagcgtct acgtgtttaa
     1501 gtaacactct aacatgtatg taaataacat gcaatacacc tgcatcaaaa ttgtttcatt
     1561 aatacggtag cgcctgagtt tatcataata taggaaatcc cttgtaacgt gttgtcatta
     1621 cgctacatta aaatttctgg ttcaacgctg tatacttgcg ccaacatctc gccacataca
     1681 acaccatcca tatccccaac atctcgccac ataacaactt caggtacaat gcccacaaaa
     1741 ttcccacaac atcacaatac aaatcaactt caggtataac tacataatat ttccacgaca
     1801 tcacaataca aattaacttt gtctaagttc cctatactat cacgccacaa aaaacactca
     1861 aatgacaaat ataataacat cgtcactcaa gtgttaattt ttaaaatctt caattatgtt
     1921 aaagcattga aaaagcgaat aatattttct tctaaaatat ctctgccttc tccatgcgtc
     1981 attaaatgat gagaatcctt cagaccattt aattctttgt ctactgatcc taaatgttca
     2041 taaataaagt gtgcacttgt ttcataggat ggcgcatctt taccaccaaa taatatatta
     2101 gctggaattt taacaacatt taaatttgtc atgatatcat caataaaatg ctgaaacttc
     2161 gtaatttcac cttcataatc atcaattgct gctaattgcg cactcgatgc ttgctgatct
     2221 agatttaaaa tattcgacat gcgttgacta taataaacta aatgttctat taagccatca
     2281 tcactctttt cctttggtgc tgacatgaca gcgatacgtt tcaaaggata gtgttgcgcc
     2341 aattttaatg tcattaatcc acctaaagac acacccgttg cactgataga ttcataacct
     2401 tcattgacta aaaattggta agctttctca acttcttccc accaatcatc tacattatat
     2461 gttatgaaat ctttcaataa taaaccatga cctggataat tcggtgcata acaactaaat
     2521 ccttggtcat ttaactcagc tgcaagatgc ttcacatccc gatttgtacc tgtaaatgaa
     2581 tgtaataata atatcgcatg tccatttgtg ccttttaaat aagatggact cggtgtttta
     2641 attctcattt ttctatatac ctccactatg tctaaagatg tttgctaaac gcgttgtcgt
     2701 cgatgattaa taaagtatgg gtataccatt aagaataacg ctacccaaat gattgctagt
     2761 gacgtgccgc caatgacatc tgaaaagtaa tgcgcatgaa aataaaggcg acaaaataat
     2821 atgctaagcc ataatattcc cataaccagc gcactcaaca cttttgtcat tgtcttagca
     2881 gcaactgaaa taataatgat cattaaggcg aaatataata atgtgctggc gttagaatgt
     2941 ccactcggaa atgaaaagcc tgtatcaacg gctaaatgat tatatggtct tggacgtaaa
     3001 acagtatctt taattaattt gttcatgatg acacctgaaa ccaaatatgt cacaaaccaa
     3061 accgctaaat gcctctgttt aataaacagt atgattgtga cgataatgga aatcaacacg
     3121 acacctttga catctccaat ttccgcacta aacgtcatat agtaattaaa taaattgtta
     3181 acatactggc gttgtggctc accgaaataa tctgtaaacc atgttaatga tcccatatct
     3241 atatttttaa gccattcttg atttgtcact acactgtaaa acatacctat aaatacaatc
     3301 agcgcgatta aaaataaagg cactgtcatt ttcggtgatg ttaatttttt atctatcatc
     3361 ttacaatctc ctcgtatcat cattttcatt ttacaaatgt tatccataat atcaatgtgc
     3421 cacaaatttc actttaccga caatatcaaa attataaagt tcatattgtt atgtatattg
     3481 caaataaaac attgtataat tgaaataaca atattttgct attttcaatt tagtacgatc
     3541 tatatttatt atacagaggg ggtagggcgt atcaatagag taatttttgt ctatatagcg
     3601 ttaatcatta cattagttag attctttacc cctatacatc catcgttttc aaatttgatt
     3661 tactggatat ttgtattata ttttattcct attatactat gcgttatcgg tttcaaggcc
     3721 gaaaaactta ttgcaacaat ggtcattata cctaattttt taggtatact ttatcgatta
     3781 tatgcctacg tcacacatat actctttatg taaaaggata gtgggcatgt ctcgcaacaa
     3841 atatgcttgc gttgacttgt caccattcac aaattttatg acatacacct ttcacgggct
     3901 atattcaaac caatgcatac atacacatga taaacacatc atataataga aaaacagcac
     3961 acccaaatat atggcgttgc gctgtttaac caagcatact tctatagctt taataagcct
     4021 gcagaagcat acctaacctt cttaaatatg cttttccaaa ttatcctcaa gtttgaatac
     4081 gataatacgt tcacctgtaa ctgtacttaa atcactatgg aagctcatca ctttgatacc
     4141 tgtaatttta aaaatgatat cattcaaatc ttgctcaccg gattcaacta attcagaacg
     4201 tgttcgttta atatttaata atccttcatt cgtactacac acacgatatt ctgctggcgt
     4261 taagatacct tgtaaactaa taatcaccat atctcttaaa atgtctgatt taaccgacaa
     4321 ggaacctctg ccaaggaaat ctttttccca ttgcgtaatg gctttactga tttcagcttc
     4381 gatttcacct ttcgttcttt tcatatcact aactccaata atattaaaat tgattacttc
     4441 atctttgtat cgttatcacg acatcaacta taacattaag tttatcattt ttagtatatt
     4501 ttaagaagct agaacattgt agatatgatg atatattagt tacttagcat cgcaacattt
     4561 catcgttaaa tccaactttt aaaacgccct tcctcattaa cgctcattaa acgcagccaa
     4621 tgattagaca ccttcctagc gaaatgctca ttattcgcga gtagtctagc tacaacaaaa
     4681 tcgggtgcct gaacaacgac aagcaaacga attggcgaat gatacatcgt tcgatcagca
     4741 gccataacag attgccatga taagccatac atcagatcac tcgcattacc ttgcatgaca
     4801 ccaacacctg acgtgacggt ttgtgtcgct ttattcccac ttccgtaaaa atgcggcgca
     4861 actgtcgacg cataatattg taaattaatc cattgtgcca caagcgccgg accagaaatg
     4921 atggtattta acaatttgcc atctttatct ttacgccaat cataattgtg taaaaatgtc
     4981 cgcccttcta aatcaatgcc ttttgttaat tggcgtcgtc caattataaa tgatgcattt
     5041 ttagccaagc cccattctgg acgtacctca ctccaatcac ttgcaaaccg ctgcgcttct
     5101 tccactggat gattcacacg accaatcgtt ggcagtttgt ccaaacgttc gcgattcgcg
     5161 tgttcagaaa tcatcggcat gacgtcattc aatgattcat atgcatctag agctaaagct
     5221 gataatgtgt ctggcacata tacccatgcc aatgtatcag tagacgtatg atgttctgct
     5281 gccgcaaaaa cagtagtctc tggaatatac acgcctgctt gttttaatcc ttgtctgaca
     5341 tttggacgat tacatatcat cgctaataac ttagcattaa aaccgcttga tgcaccacca
     5401 caagccccac attcaagtga tgcgtgatgt ggattattgt gagaatgact agcgtgacct
     5461 gctaacacaa cgaacggcgc aaatgcttcg gttaaatcca tcaatttcaa cgcttgtaac
     5521 gcgaaatcaa tttgctcttg ttcagtaaat ccaacaggta agtctgatgt tctgtcaaac
     5581 tcacgatcaa tcgttaactt tgtttcaggc ttttttaacc atttttgttt tattttttgt
     5641 aaagacacgc gactttttct aggcataatc gaattgacaa tggtactcaa gcttaaaaat
     5701 ggcccactta attcaggcaa taacagacta ggcataacat tatttttcat caatttaaat
     5761 gtgtaaaaca tcgatgacat tgtctgttgc tgttgtcgat aaacattcat atcgtagcgg
     5821 tctgcaaatt ctttaatgcg atatgccggc ggtaccatga caggtaatga atcatgtttg
     5881 aattgttcgt ctacggcatc tttttgaata ggtaatccaa aaaaacctgc aataccaatc
     5941 gtttcaaagg gccctgctgc ttcgatatgt ctacgaaatg gttctgaacg aacatctata
     6001 caaaatgcaa tttgcgcttt cgttgatgtg cccacctgat ttagctcgct attattttca
     6061 tcaactgctt gtgtggcatt taacgatact gaatgtgact gattagcgtt gtcattttca
     6121 gtaacattta cttggtttac atctaatgcg cccgccacac tttcatgacc tgctttaatt
     6181 ttttgtttta actgagattc gtatgtcatt tcccaggcaa tcaaccataa atttttaaat
     6241 acatttttat tcatagttgc tgcaaaatga ataaacgttt gaatttcatt gacgtcatgt
     6301 tgtagtaata catcgctagg catatcactg tagtaacacc atgatgcaac agtttgctta
     6361 aaccaatttt ccgatctgct ttcacaatct ttagtgactg acttaaactc atcaccaact
     6421 agcaattgtt cgacaactaa ccgaatggcc aaataatccg ttaacaaatg ttgttcaaag
     6481 tgatgctgtt gtgagcggta gtataatata cctgcccaac ccggtaacgc caaaagatgt
     6541 ccttcaacat aagcttggta gtcttcctgc gctattgaaa aatgatttaa tactgactct
     6601 atcgtcattt taggatcatt gggtaagcct ttaatcactt ggcgctgtgc tttagtaaaa
     6661 ctatggtcat gttgcgctaa atgcaaccat gcatggtaaa aactttgctc acgcttcggc
     6721 attgtccaac tcgatagaaa ttgatcgata taaagtttcg tccatttaat catttgacga
     6781 ttcacttgtt cgctaagtgg ctcaccttgt tcatctatta ttgcatcact catcggacgt
     6841 acatcatagt gatgatatga ttcagccata tcacgttttg atttttctaa tagtagatca
     6901 gcaacaacat caacatttga atgattcatg tatgatgcag gtacgtcttt taatgtttta
     6961 atgttatcaa tataaagatt gatgtagtgt tgcgggatat tgtagtgatg ttcaagtagc
     7021 atatcagtaa caagttgatt aaagacactt tcgtctaatt caccgcgtgc cacagcactt
     7081 tctattaatg ctttatttgg gaaaatatcc acatcgcgaa catcacgtaa ccattttgcg
     7141 acatcttcaa acgtatccgc ttctaatcct tcccatggat ttcgtgctgc aaaaatcgaa
     7201 atcggtgata atggtgtaat aacacgtttc gcattttcaa tgactgaatt gatatttaac
     7261 tgtgttgtca tacctttcac ctcctataaa tacttcttca aataattcgg atgactttct
     7321 atcgctttcg agcgtgcttc tcctagatta actaaccaca cgtacaatac cgcaaaagtc
     7381 ttagagtatc gatgacgcgc tacccagata cttaataaac taccaaatat taatatgaca
     7441 acactaatga tgacactaat tgtaggcggc gttgtcgcat gtgttgttat attttgtaat
     7501 acatcgtaaa aataattatg tgtgatgatg tagataaatg tcacaattga aatcaaaatc
     7561 atgccaacca gacgtgccat acgcccttta ctaaaggcta ccatttgatt ccaagataca
     7621 agtaatgacc atgctagaat gagtgcactt aacacttcat atgcacttct gtcactactc
     7681 atccaaaaca gaaatgccac gataatagct aatacacgtc ccatgacaat ccagccataa
     7741 gcgtctttag cagatgcttg ttttggaata ttgaatcgct tcacgataga acctgattgt
     7801 aaaaataatg ttgctttaaa aataccgtgc aatattaaat gtataatcgc tgctgaatat
     7861 acaccaagtg cgcattgaac taacataaag cccatttgac tcatcgtaga gcccaccaat
     7921 tggcgtttat agtcaacttg aaccaagcta atacccgatc ctaacaatac agaaatgctt
     7981 gaaaggataa gtaataatga taacgcaaat ccattatcaa atatcggcgc aaaacgagtt
     8041 agaataacac cacctgcatt cacaattcct gcatgcataa ttgccgatac tggcgttggt
     8101 gctgttacag attcaatcaa ccatcgatga aaaggaaatt gtgctgccgg tatcatgaca
     8161 gctaatacaa gtagtacatt cgtcaacaat gaccatgtcg gatgcgctat atgttgtggt
     8221 acccgccact cgccagttgc aatataaata gatacaattg ctccaacgaa tgcaagccaa
     8281 ccacataaaa atgtcatgct tgataatttc gcagactcac gtggcacttt ccaaaaacga
     8341 ttaacgttca tcagcaatgt taaacataat aatgtcatac cccagcagag tgccatcagt
     8401 cttaagtctt cagacatcca tgctaaagat gcaaacgatg taatcgcagt gaacaatgga
     8461 aagtaatgtc tataatgatg atcacctagt aaatatcgca ttgaaaactt ttgaataata
     8521 aagccaagcg ccattacaaa gccagctaat aaccaagata aacgatctat tttaaatgga
     8581 cctaagacat gttgaccatg aataccgaaa aagccaatga ctgcaaataa tactggcatg
     8641 actagtatgt ataaatgtaa tttaatatat ctcattggca taactggtgc taaaaacaac
     8701 aagccactta tcaatgcaat gataagcgca ataacaaaca gtgaaaatag caattgaaaa
     8761 cttaacactg cataacctcc ttatttctaa tctctcgcat aatgcttatg tataaaaaga
     8821 aaaacctaca atagtagatt ctgtacataa tggcagaaaa tttactattg caggtttcag
     8881 tttaactaga cactgcacca tggtatgttg atataccttg ttacactgtt ctctttaagc
     8941 gtgctcccat gcacatatgt atataaaatg ttacttctgt ctgttcaatt catcttcata
     9001 aatatgcttt gcctagacga gacctaacgt gttattcgtt ttaaacttat aacataaaat
     9061 ataattaaat ttctgcttca tgtcaaattc atgaccttaa ccccactaaa ctaatgattg
     9121 taaagatttt gtaaatgcac ctgtacagtt aggcagtatt tcccgtcctt ttaaaataaa
     9181 aaattcgcag ttatgatcat aacaattcaa gttaggaaaa aaatcaatta cgcacaagat
     9241 aactatgtac aatgaagtta actcataagc aaaggaggta atcttaatgg gtatcatcgc
     9301 tggcatcatt aaagttatca aaagcttaat cgaacaattc actggtaaat aagttttcat
     9361 aacaaacaaa ggaggtcttt cacatgggta tcattgcagg aatcattaaa ttcattaaag
     9421 gattaattga gaaattcact ggtaagtaag ttataaaaat atcatagata tgaacatctt
     9481 atttgaaggg ggccattcac atggaattcg tagcaaaatt attcaaattc tttaaagatt
     9541 tacttggtaa atttttaggt aactactaat ctcaaacatt aacgatcaac aactcatcac
     9601 tatgttaaat caacatacag gaggacaaaa cgatggctat tgtaggtact atcattaaaa
     9661 tcatcaaagc aattatcgac attttcgcaa aataatttaa gcgaattgaa tacttaaaat
     9721 tctcaggcca ctataccaat agggatggtg gctttgcttt gtgttgattt attgatgtga
     9781 gatgagtctg ggtagtttgt tgcaaataaa tggtcttggt gttttttgta taggatgttc
     9841 ttagtgggac gtacggaata ttcgtgatct ttgtagtctg acgcgttata tttttgtggc
     9901 gtgttttatg tttgatactc gaggtctgag acattcatga tttggcatgt gaaatcttaa
     9961 tgatcttcat gatctagcgc atgatatatt ggacgcgtgc agaatttcat tgcacgttta
    10021 ggctgaaaca ctcgtgtgac tgtaagcagt aatagtacat tgatagctgc atttgcttca
    10081 ctcattttta tgacctttaa acaatgattg tactttcaat taacagttgg tacgatggtt
    10141 ttgtcatttt tcatcaacgc aaatataaaa aggactaaga cacatacatg tcctagccct
    10201 atggataaaa tgcaaatttc tgctttatca aaactatcac actttagata gattgaaaac
    10261 aaaaagatcc taagaacatc ttaacttttt attaattgtc ataaattgca aacaattaag
    10321 ccacaattta aaaatgatta cacttcattc aacttatcgt gccggtctaa tttgccattg
    10381 atatggatct tcaaattgtt gccaatctgc atcaatttct tgcgcattga ctaagcatgc
    10441 atcgagttct tttgtaattg caccttcatc taagtctgta ccaataatga caaattgtgt
    10501 atgacgatcg ccatattctg gatcccattc agctgcgacg tcttggcgtt ctgctaatat
    10561 ttgcgtttgt tgcgcttcag acatactagc cacccaatat gtaactggat gaatattgca
    10621 agatgaccct gcttgagata ataaacatgc tacatgattg tattgtgcta accatacgat
    10681 accttttgat cgaacgacat tatttggcat gctttctaac caatcattga accttttagc
    10741 atggaaaggt agacgacgtt tatatacaaa cgatgatata ccatattctt ctgtttcagg
    10801 tgtatgcgat gcatgcccac cagactcaag ttctttgatc catcctgctg actcgctcgc
    10861 tttttcaaaa tcaaaacgct gcgtattcaa gacttctttt aaatctactt cagaatttgt
    10921 tgtcttaata attttagcag tcggttgcaa tgcacttaac actttttcta acttcgctag
    10981 ttcttcttca cttattaagt caattttatt aataatcaat acatcacaaa attcaacttg
    11041 gtcaattaat aaatcagcaa tcgaacgctc atctgtttca tcaacgcttt gatcacgatc
    11101 catcaataaa tcttctgagt tgatgtcatg gacgaagcgg ttagcatcca caactgtaac
    11161 cattgtatct aaacggcaaa tcgctgtaag atcaatgcca agttcatcat caatatatga
    11221 gaaagtttgt gcaacaggta ctggctctga aatccctgtt gactcaataa caatttgatc
    11281 gatgccacct tttttcacta aacgctcaac ttcttttaat aaatcgtctc taagtgtaca
    11341 acagatacaa ccgttagaaa gttcgactaa tttttcgtct gtgcgcgata gtcccccacc
    11401 atctgcgaca agatctttat cgatattaac ttcactcata tcatttacaa ttactgcgat
    11461 acgtcgacct tcacgatttt gtaaaatatg atttaacaac gttgttttcc ccgagcctaa
    11521 ataaccactt aataccgtaa ctggaatttt agccatatat ataccctcac ttcaatttat
    11581 ttgtaaatag gaataattcc gttttacatt atataggagc atttcacctt tcgcaatctt
    11641 cgataataaa aaaatagtat acttaattaa attattgagc actttacttt ataatagaga
    11701 caaagatata tctcacgaga gagaatcgag gtgtataaac atgttatttg tcattttagt
    11761 tttatatatc attggtattg catttattct actcagtgtt tttggttcaa agactgaagg
    11821 attatctacg aaacatactt tatataccat tggcagtgct attataacga ttgctatttt
    11881 tatttcaatt ggctatgcca ttcaatactt aactgcagcg ctttatggtt tgtaagttga
    11941 agatgattta tggcgggtag ttcaggagag gttaacttgc gttgattttg ataaagtggt
    12001 catagctttt ggtacttgag gattttgttg ttgctgtttg gaatgtggat atgtttaatg
    12061 cgggacagta atttaagttg tgtttttaca attgtgagtg tgatatttcg attaagagag
    12121 cgttacttta catggaaata aaataaatta aaaagaagcg gcctagtgtc agttgtgaat
    12181 acactgaaca ttggtcgctt tatttagtat aatgatatgt agtttagcta ttaatttttt
    12241 tcaggtcatc cttaatgctg tctatctcag acatggcact tttaacccaa tctccttgag
    12301 ctgcgccttt aaaattagct ttaaaagcct cgcaatgttg cgccatttgt tcaattaata
    12361 ctttttcttc accttttaat ccgttttcaa tatctttgta tttatgctta tgttcaggtg
    12421 caataactgt gcgaatattt tctttttgag cattcatttt agatatgaga ttaagtgttt
    12481 ctactgtagt acttatatct ggcattctta aggtcatatc tggttctatt agagtcattt
    12541 aatctcctcc aaattatcag tcacttagtt tatctaactg cttttcataa gactttttta
    12601 agtcttcttt atattcttct aatttccatt cttgctttct aattatctca ttgatttctt
    12661 gctcatacgc ttctatattt ctcgtcattt tcctttgggc atcttcacta taattcattt
    12721 cgtgcttcaa atattgaaaa acatcataac tacgttcatt ttcttttcga gttctatgcc
    12781 taagttcatt taaattttgc agttcattct ctatacgttt tttctcggat tcatatgatt
    12841 cttctaattc atttttatta ttaatttgat taaacatcat agtaatccac ctatttgtga
    12901 tgcaagcact tgatctttat ctacgatttc attaatactg agtctaactc tcataatata
    12961 atttgataca gaagacccta tcgttgaaag ttgtctttct tttgcagata tcatttgttc
    13021 aggttctctt accatactac tttcattcac ttgaccttca tctaaagcat taagaacttc
    13081 atttacactc aaatcttcac ctacagcttt tccagctttt tgagcgtctt cccaattttt
    13141 tccaaatttc tttttctttt cattatacat ttttttgatt tgattaattt catcgtctat
    13201 cagttgactg aatgactgcg caattgttaa agctgttaaa ctgtctaaaa ctttctgctg
    13261 cgaagaagat aatcctcctc catttgcagt catcatattg gctctaagta attctatact
    13321 agctaattta gagatagcat tatttgaatg ctttacaaat gacttattat tttcttctgc
    13381 ataacctttt agttttttaa gttctttttt tatagctttt tgttcttctt cggtcagaaa
    13441 gccttccatt tcatgacctt taccattttt aaagacttta tcattaccga aaaaaacata
    13501 atgcttatat ttcttcagat tttctgtcaa tgcatcattt tctgcaacaa acctagtaat
    13561 attaccttta taatttttat ataattctaa caatcgtttt ccatctgggg aattaggatt
    13621 caaactagtt atagaaatag gagctggatt ataaaccaca atgttcggaa taccattact
    13681 cattcctaga actaccgcat ctcttccacc tagtgaatgt ccagtaataa aatcaatgtc
    13741 atacttactt tttattttat ttataaattc ttgtgtattc gcatatctat agttttgatc
    13801 agatgcagga gaatataata ttttaatatc tgcatatcca tctttaactg tttccaaagc
    13861 attggtaaca ttttgtcttg aaaattcacc ttcttttaac tttttaaagg cttcgccttg
    13921 tatattagtc ccagtcatcc caagagttac tttgcctgta tctttattta aaaaagcaga
    13981 agttgttact ccagtaagag aatcagtgta actatcaaca tattcaagat tagaagggaa
    14041 catgcccctt tctgcgcttc tatctaactg ttcctttatt gccatcttac tcaaattatg
    14101 attattagca ttttcaattt catacgccat aaatgatatt gcgcttgtcg gttcagtatc
    14161 ttttttaatt tgccaacatc taatttatag acactcttac tcattatagt ctccttcact
    14221 cggtttaaac tctatactat caatacaaga aacactttct tctttgttcc tattgaaaat
    14281 tatctgtatc tccttatatc caacagaaga cccttttata ttacctgctc ctttgaacaa
    14341 taacttagga tttttattag acttaatatt gaatcgattt tttaattgtt tgatgttatt
    14401 atcatcatca ttaattttat attcagctga atagttaggc gcattaggat tataagaaat
    14461 atttgttatc ctatccgtat ttaaactctt aaattcacca tattgagaaa ataatttgaa
    14521 gttttcaatt tctcttttta aatcttcgtt ttttacatct tcagttggaa ttattttatt
    14581 attcaccaat ttaactggat actttttttc gtttttttga acatcatgat ttttatcttc
    14641 tttcaattct cttataatat aacttccttt agcagttctc ttatttctat ctaactttaa
    14701 tatcattcct cgtgatttta aactctcgcc tttgaattct gatacaatag atgaatacag
    14761 aacccacatc cctttgtcac ctttttcaaa ttcgccatct cgatagcctt ctttgtcgta
    14821 taaatcctcg agattcttaa ttggatacat atctaacgtt ttcgcaaaac tctttttgat
    14881 ttgttcttct tttgaatctg ttttatttgt ttgttcctct tttttctgtt catctttcat
    14941 atttccacat cccccaataa aaatgattaa aagtaataca ctcatgtaca aagcaatttt
    15001 tttatatact ccatcatttc acatctctct gctttatttt tatacacatt tgaatatttc
    15061 atacaactta ataaaatagt gattattaca cttttatgtt atcaaacaaa actaacttat
    15121 tcaatttaat acatgtaatt catttaactt tattattaaa tgttctaacg ttatttttta
    15181 tattagtaat tatatttatc ctcgcttgga ttaaagtaga cactatctgt gaagtaaata
    15241 ttttctccct tattttcaac aaaggtaaat tcaatatttt tatatccaac tgatgagcct
    15301 tttagattac ctgacccttt caataataat ttaggcgctt tttgcgtcgg gatattatat
    15361 cgcttacgta attgttctac attgtaatca ctgtttttca attgatattg tgcagagtat
    15421 attggtgctt ctgggttata tgtcacttct ccgtctttat agttttccaa ttctttgaaa
    15481 ttcccgtatt gtacaaagaa tttaaattct tcaatttctt tttttacttt ttcatcaacg
    15541 atttgtttta atggaatgat tttattattt tccattttta caggatacct ttttgtatga
    15601 ttgtgtgtca ttccatcgct atcttcaaca acttctctaa caatataatt ccctttagcc
    15661 gttctagtat ttctgttaat ttctaaaact actcccctag attccagatt ttctcctttt
    15721 aattggattt tcatttcaga tctaataacc caagtccctt tatcaccttt tttaaattct
    15781 ccgtctcgat atccttattt gtcgtataaa tcttcgaggt ttttgattgg atacatttct
    15841 aatgttttcg caaagctctt tttgatttgt tcttcttttg agtctccagt aatcttatta
    15901 tcaccatcac aagatgtaac aaagatgatt aaaatcaaca aactaatgca caatccaatc
    15961 ctttttatag acttcattgt tacacaccat cctcatcatt catcatcttc attcaccttc
    16021 gccggctgga aattcaaaga atctattact gacaaatcgc ttttttcacc ttttgaaaag
    16081 tcaatttcca actttttata acccacagaa cttcctttca aattaccatc aatatgcatt
    16141 tttaataccg gtgctttatc agtaggaata ttataacgac ttcttaattg cttaacattt
    16201 tcatctttat tgctcatttt aaattttgcg tcataacttg gaacattctc attaattgag
    16261 acatcaccat tgttgtaatt tttcaattct ttaaggtttg catattgtcc gaaaaattta
    16321 aagttttcta ttctcttttt tagttttgga tcttctacct tgtctaataa gataatttta
    16381 ttgtttttca tttcaacttt atattttttt ctatctggga atttatcctt ttcataaaat
    16441 gtcttaacaa aataatgtcc ctttgctgtc cgtgtatttc tatctaagta taagaccata
    16501 ccttcattac tcaatccacc ttgtttatta cttttggcga aatctgtata tatcgtccac
    16561 atccctttat cgcctttttt aaactcacca tcacgagatc cctctttgtc gtataaatcc
    16621 tcgagattct taattggata catatctaat gttttctcaa aactcttttt aatttgtgct
    16681 tcttttgaat cttcttttgg cttttctgaa gtatcgcttg atttatcaca acctattata
    16741 aaaacactca aaaccataac aattatgtac agtacaactc tatttaaata tctcatcatt
    16801 tcacacccta atatttaatt atcaacacta ttttttcgct ggcttataac ttaaataatc
    16861 aatgactgaa atatccctat cttcttttga aaagtctatt tctaaccttt tatatccaac
    16921 agaacttcct tttaagtccc cgtcaatatg catttttaac attggagctt tatcagtagg
    16981 aatgttataa cgacttctta attgcttaac attttcatct ttattgctca ttttatattc
    17041 cacgtcataa cttggaacat tccaatttat tgacacatcg ccattgttgt aattttccaa
    17101 atccttaaaa tttgcatatt gtccaaaaaa cttaaaattt tctattcttt ctttaaggtt
    17161 tggatcgttt accttgtcta ataaaataat tttattgttt ttcatttcaa ctttataatt
    17221 ttttctatct gaaaatttat ccttttcata aaatttctta acaaaataat atcccttagc
    17281 cgttcgagta tttctatcca gatttaaaac cataccttca tcatccaatt cgtctgattt
    17341 attgctttta gcaaaatctg tatatatcg
//
LOCUS       DAMZPP010000053        17275 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMZPP010000053 DAMZPP010000000
VERSION     DAMZPP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17275
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.053"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            66..971
                     /locus_tag="POH73_001319"
     CDS             66..971
                     /locus_tag="POH73_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDI6887282.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEMAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGADVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QHIDYFVAGIGSGGTYTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERSLVDGIFTIKDQDAFRNVKSLAKHEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKEIFNYEENDYE"
     gene            964..2106
                     /locus_tag="POH73_001320"
     CDS             964..2106
                     /locus_tag="POH73_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDI6887283.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIASLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGVEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTLYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            2397..3422
                     /locus_tag="POH73_001321"
     CDS             2397..3422
                     /locus_tag="POH73_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDI6887284.1"
                     /translation="MIEFRQVSKSFHKKKQTIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGQ
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSPSLIRRLNDKQVGDFKDYKIFVEETQ
                     MTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFDGTAINQYFKEKN
                     IQFEEVH"
     gene            3426..4085
                     /locus_tag="POH73_001322"
     CDS             3426..4085
                     /locus_tag="POH73_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6887285.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPIINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVVIVQVIQTLGNVLARFIRR
                     H"
     gene            4122..4964
                     /gene="gmpC"
                     /locus_tag="POH73_001323"
     CDS             4122..4964
                     /gene="gmpC"
                     /locus_tag="POH73_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HDI6887286.1"
                     /translation="MKRLIGLVIVALVLLAACGSNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGVYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSDSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            5298..6302
                     /gene="aaa"
                     /locus_tag="POH73_001324"
     CDS             5298..6302
                     /gene="aaa"
                     /locus_tag="POH73_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HDI6887287.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNSTSNSSRPSTNSSGGSYY
                     TVQAGDSLSLIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVTGNASTNSGSTTTTNRG
                     YNTPVFNHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(6489..6758)
                     /locus_tag="POH73_001325"
     CDS             complement(6489..6758)
                     /locus_tag="POH73_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887288.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            6908..7303
                     /locus_tag="POH73_001326"
     CDS             6908..7303
                     /locus_tag="POH73_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDI6887289.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESPVHALL
                     REVKEELNLTLTMDEIEYIGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYDGKHDK"
     gene            7293..7778
                     /locus_tag="POH73_001327"
     CDS             7293..7778
                     /locus_tag="POH73_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6887290.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRKRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(7947..8729)
                     /locus_tag="POH73_001328"
     CDS             complement(7947..8729)
                     /locus_tag="POH73_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDI6887291.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMIQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(8726..9844)
                     /locus_tag="POH73_001329"
     CDS             complement(8726..9844)
                     /locus_tag="POH73_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDI6887292.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMTDGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            complement(9959..10843)
                     /locus_tag="POH73_001330"
     CDS             complement(9959..10843)
                     /locus_tag="POH73_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6887293.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYNESDV
                     SHMLLPLIQTFHLQNDTHVIPNLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLGPFAESVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSCIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            11024..15523
                     /gene="gltB"
                     /locus_tag="POH73_001331"
     CDS             11024..15523
                     /gene="gltB"
                     /locus_tag="POH73_001331"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDI6887294.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKH
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKFYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGNLEDALEVLGREAVDAVKQGAQILVLDDSGLVDGNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKERQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATGGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            15541..17004
                     /locus_tag="POH73_001332"
     CDS             15541..17004
                     /locus_tag="POH73_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HDI6887295.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDFVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLTAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGIT
                     FVNGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQVLEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
BASE COUNT         5831 a         2537 c         3569 g         5338 t
ORIGIN      
        1 attaaaattg agacatgaag aaaggtttca ttcatttgat cggcatgtca gatgagggtg
       61 catctatgat tacttatgat ttaattggca atacaccatt agtactgtta gaacattata
      121 gtgatgataa agttaaaatt tatgccaagc ttgaacaatg gaatcctgga ggcagtgtta
      181 aagacagact cgggaaatat ttagtagaga tggcaattca agaagggcgt gtgcgtgcag
      241 gtcaaactat tgttgaagcg actgctggca atacaggcat agggttagct attgcagcaa
      301 atagacatca tttgaaatgt aagatctttg cgccgtatgg tttttcagaa gaaaagatta
      361 atattatgat agcgcttggt gcagatgttt caagaacgag tcagtctgaa ggtatgcatg
      421 gggcacaatt agctgcacgt tcatatgctg aaaaatatgg tgccgtttat atgaatcaat
      481 ttgaatccga acataatccg gatacatatt ttcatacatt gggacccgaa ttgacttcag
      541 cattacagca tattgattat tttgtggctg gtattggctc tggcggtaca tatacaggta
      601 ccgcacgtta tttaaagcaa catcacgtgc aatgttatgc cgttgagcca gaagggtccg
      661 tgttaaatgg agggccagct catgcacatg acactgaagg tatcggttct gagaaatggc
      721 cgatattttt agagagaagt cttgtagatg ggatatttac gattaaagat caagatgcct
      781 ttcgaaatgt caaaagtttg gcaaagcatg aaggattatt ggtaggcagt tcttcaggtg
      841 cagcattaca aggtgcattg aatttaaaag cgcaattatc tgaaggtacg attgttgtcg
      901 tatttccaga tggtagcgat cgatatatgt ctaaggaaat atttaattat gaggagaatg
      961 attatgaata agaaaacgaa attaattcac ggtggacaca caacggacga ctatacaggt
     1021 gcagttacaa caccaattta tcaaacaagt acatatttac aagatgatat tggtgattta
     1081 cgtcaaggat atgaatattc tcgtactgcg aatccaacaa gaagttctgt agaaagcgtt
     1141 attgcgtcat tagaaaatgg caaacatggc tttgcattta gttcaggtgt tgcagcaatc
     1201 agtgcagttg ttatgctgtt ggacaaaggt gatcatatta ttttaaattc agatgtatac
     1261 ggcggtactt atcgcgcatt gacaaaagta tttacacgat ttggcgttga agtggatttt
     1321 gtagatacaa cgcatacaga ttcaattgta caagcgatac gcccaacaac aaaaatgtta
     1381 tttattgaaa caccttctaa tccattatta cgtgttactg acattaaaaa gtctgctgaa
     1441 attgcgaaag aacacggttt gatttcagtc gttgataaca catttatgac actttattat
     1501 cagaatccat tagatttagg catcgatatt gtcttacatt ctgcaacgaa atatttaggt
     1561 ggacatagtg atgtcgttgc tggtttagtt gcaacatcgg atgacaagct tgcagaacgt
     1621 ttagcattta tttcaaattc aacaggtggc attttaggac ctcaagatag ttatttactt
     1681 gtgaggggga ttaaaacatt aggtttacgt atggaacaaa ttaatcgcag tgttattgaa
     1741 attattaaaa tgttacaagc acatccagct gtgcaacaag tgttccatcc aagtattgaa
     1801 agtcatttaa atcatgatgt ccatatggct caagcggatg gccatacagg tgtgattgct
     1861 tttgaagtga aaaatacaga aagtgccaaa caattgatta aagcaacatc gtattacaca
     1921 ttagctgaaa gtttaggtgc agtggaaagt ttaatttcag tacctgcatt gatgacacat
     1981 gcatctattc cagcagatat tcgagctaaa gaaggtatta cagacggact tgtaagaatt
     2041 tctgtaggta ttgaagatac tgaagattta gtagatgatt taaaacaagc actagatacg
     2101 ttataaataa tagcagcact ggcatatatt ttgagttcaa cgtttgttgt tcttgaatat
     2161 gctagtgctt ttttgtgtgg tgttaatatt tcgtaattgc attgtggagg attgctggtc
     2221 atttttattc atttaatttg ataaatgtca tagtagtctc acaattcgtc attgtcacaa
     2281 ggtattattt tttaattttt tagaaaattc ggcaaattgt attgacgata tatgcaattt
     2341 tacatataat gctcttattc ctagtggatt aataagaatt tgtaggaggg gcgatgatga
     2401 ttgagtttcg acaagttagt aaatcatttc ataagaaaaa gcaaacaata gatgctttga
     2461 aggacgtatc atttacggtc aatcgcaatg atatttttgg tgtgattgga tatagtggtg
     2521 caggaaaaag tacgttggta agactcgtga atcatcttga agctgcctcg aatggacaag
     2581 tgattgtaga tggacatgat attacgaatt atagcgataa aatgatgagg gatattaaga
     2641 aagatatcgg tatgatattt cagcatttta atttactaaa ttcagctacg gtatttaaaa
     2701 atgtagctat gccgctcatt ttaagtaaga aaagcaaaac agaaattaag caacgagtaa
     2761 cggaaatgct tgaatttgta ggattgagtg ataaaaaaga ccaatttcct gatgaattat
     2821 ctggtggtca gaagcaaagg gtggctattg caagagcgct tgttactaat ccgaaaatac
     2881 tcctatgcga tgaagcaaca agcgcattgg atccagcaac gactgcttcg atattgacgt
     2941 tattaaagaa tgtcaatcaa acctttggca ttacaattat gatgattaca catgaaatgc
     3001 gcgttattaa agacatttgt aatcgtgttg ctgtaatgga aaaggggcaa gtggttgaaa
     3061 caggaactgt taaagaggtg tttagtcatc ctaaaacgac gattgctcaa aattttgtgt
     3121 ctacagttat acagactgag ccaagtccat cattgattcg tcgattgaat gacaaacaag
     3181 ttggcgattt taaagattat aaaatcttcg tcgaggaaac tcagatgaca caaccgatta
     3241 taaatgactt gattcaaatt tgtggcagag aggttaaaat tttattttca tccatgtcag
     3301 aaatacaagg taacaccgta tgttatatgt ggcttcgatt taatatggat caacaatttg
     3361 atggcacggc aataaatcaa tatttcaaag agaaaaatat tcaatttgag gaggtgcatt
     3421 aacatatgtt tggttctgat ttagacagtg cacagttatt acaagcatta tacgaaacac
     3481 tatatatggt atctattgct ttatttttag gagcagtgat tggtattcca ttaggtgtct
     3541 tattggtaat tactcgaaaa caaggcatat ggcccaatat agtgatacat caagttttaa
     3601 atcctataat caatatttta aggtcactac catttattat tttgttgatt gcgattgtgc
     3661 cattcacaaa attagtagta ggtacttcca ttggtacgac tgctgccatc gtgcctttaa
     3721 cagtatatgt ggcaccttac attgcaagac ttgttgaaaa ctcattattg gaagtagacg
     3781 aggggattat tgaagcggcg aaagcgatgg gcgcttcacc actacagatc attagatatt
     3841 ttttaattcc tgaagcgtta ggttcattag tattagcaat taccactgcg attattggac
     3901 ttattggaag tacggcgatg gcaggcgctg ttggcggtgg tggtatagga gacttagctt
     3961 tagtgtatgg ttatcaaaga tttgatacga cggtcattat tattaccgtt attgtattag
     4021 tcgttattgt ccaagtgatt caaacgctag ggaatgtcct agctagattc atacgtagac
     4081 attgatgata tatagtgaag attttgaaag gaattgatag aatgaaaaga ttgattgggt
     4141 tagttatcgt agcacttgta ttattagcag cgtgtggtag taacaatgat aaaaaagtaa
     4201 caattggtgt cgcgtcaaat gacactaagg cttgggagaa ggttaaagaa ttagctaaaa
     4261 aagatgatat tgatgtggag attaagcact tctccgatta caatttaccg aataaagcat
     4321 taaacgatgg tgatattgat atgaatgcat tccaacattt tgcattttta gatcaatata
     4381 aaaaggcgca taaaggaaca aagatttcag cattaagtac aacagttttg gcaccgttgg
     4441 gcgtttactc agataaaatt aaagatgtca aaaaggttaa agatggtgct aaagttgtca
     4501 ttccaaatga tgtgtcaaac caagcacgtg cacttaaact attagaagca gctggtttaa
     4561 taaaactgaa aaaagatttc ggattagcag gtacagtgaa agatataacg tcaaatccaa
     4621 aacatttaaa aattactgca gtagatgcac aacaaactgc acgtgcttta tctgatgtcg
     4681 atattgcagt tattaataac ggtgtagcaa ctaaagctgg gaaagatcca aaaaatgatc
     4741 cgattttctt ggaaaaatca gattcagatg ctgtaaagcc atatattaat attgttgcag
     4801 ttaacgacaa agacttggat aacaaaacat atgctaaaat cgtagaattg tatcattcaa
     4861 aagaagctca aaaagcgttg caggaagatg tcaaagatgg agagaaacct gttaatttat
     4921 ctaaagatga gattaaggca atagaaacgt cattagcaaa ataaattata ttacgtccta
     4981 caagcatagt tcatgcttaa gtttgtaggg cgtttttgtt tgcctataaa aatgtttcaa
     5041 aagacagaaa gggattgtaa tcattttata gtgaaatatt atgaaattgt aataatttag
     5101 atattgtaaa atctaataag ttgtaataat tttaagaggt aattataaaa tttgatgaca
     5161 cagtatatga tttttttgta atcataatgt catcaaacat caacctatta tacataatta
     5221 aatcgtataa tgatgtagta ttcataaatt cggataaaaa gaatgttagg aaagttatgc
     5281 aagaggagga ttttaaagtg caaaaaaaag taattgcagc tattattggg acaagcgcga
     5341 ttagcgctgt tgcggcaact caagcaaatg cggctacaac tcacacagta aaaccgggtg
     5401 aatcagtgtg ggcaatttca aataagtatg ggatttcgat tgctaaatta aagtcattaa
     5461 acaatttaac atctaatcta attttcccaa atcaagtatt aaaagtatct ggctcaagta
     5521 attctacgag caatagtagc cgtccatcaa caaattcagg tggcggatca tactacacag
     5581 tacaagcagg cgactcatta tcattaattg catctaaata tggtacaact taccaaaaca
     5641 ttatgcgact taatggttta aataatttct ttatttatcc aggtcaaaaa ttaaaagtat
     5701 caggtactgc aagctcaagt aattctacga gcaatagtag ccgtccatca acgaattcaa
     5761 gcggtggatc atattacaca gtacaagcag gtgactcatt gtcattaatc gcttcaaaat
     5821 atggtacaac atatcaaaac attatgagac ttaatggctt aaataatttc tttatttatc
     5881 cgggtcaaaa attgaaagta actggtaatg catctacgaa ctcaggatct actacaacga
     5941 caaatagagg ttacaataca ccagtattca atcaccaaaa cttatataca tggggtcaat
     6001 gtacatatca tgtatttaat cgtcgtgctg aaattggtaa aggtattagc acttattggt
     6061 ggaatgcaaa taactgggat aacgcagcgg ctgcagatgg ttacacaatt gacaatagac
     6121 ctactgtagg ttcaatcgct caaacagatg taggttatta tggtcatgtt atgtttgtag
     6181 aacgcgtaaa taacgatggt agtattttag tttcagaaat gaactattca gctgcaccag
     6241 gtattttaac ttacagaacg gtaccagctt accaagtaaa taattataga tatattcact
     6301 aaagtcttac gtatataaat atatataatg aattcctatt acatttcaca agctgaaaag
     6361 ttggatttaa tcctaaactt tatggcgtga ctatcaaagt cgtgattatt gtaataggaa
     6421 tttttgtata agaaaaagga ttgatcgaat gaacgtcatg ctctatgttc gaccaatcct
     6481 tttctgattt agtattcatg ctttactttg cgaatgcttg taaataatct agcaccgttt
     6541 gttattaaag taacaactgc cactgctttt ttgaacttgc gtggtgactt aattgaaatg
     6601 taaaagtcta acacggttcc tacagtgctt aaacctaatg aaagatatac taatttttta
     6661 tttttagcat gatatttata gccattgtag ccgtcgacta tgaaacctgc gacatttagt
     6721 aaacttgata aacgttgtga tttggaacgt tttgccataa taataattcc ccctagtatc
     6781 ttctgtttat atggaataat tgggtgcgct gaattgctaa cgttttgcgc tataactact
     6841 catatatgat aacataattg tacagtataa tttgaaaaat tgatttcaca aagttggggt
     6901 gtcaaagatg attaaatgtg tctgtttagt agaagaaaca gctgataaaa tattgcttgt
     6961 tcaagtaagg aatcgcgaaa agtattattt cccaggtggt aaaatagaag aaggggaatc
     7021 accagtacac gcgctgttaa gagaagtaaa agaagaatta aatttaacat taacaatgga
     7081 tgaaattgaa tatatcggga caattgtagg tcctgcatat ccacaacagg atatgttaac
     7141 tgagttaaat ggatttcgcg cattaaccaa aatcgattgg gaaaacgtaa ctatcaataa
     7201 tgaaattacg gatatacgct ggattgataa agataatgat gcgttgattg cgcctgctgt
     7261 caaagtttgg attgaaacgt atgatggtaa acatgacaaa taatgacacc atcatgttac
     7321 gacattatgt cccacaagat tattcgatgt tagaagcttt tcaattaagt gaaagtgatt
     7381 tgaagtttgt taaaacgcca gaggaaaata ttacagctgc aatgtctgat aatgaaaggt
     7441 atcccatcgt tgtaatggat ggcaggcaat gtgtggcctt ttttacatta catcgtggaa
     7501 aaggtgtcgc accatttagc gataaccaag atgcagtatt tttcaggtca tttagtgttg
     7561 atcaacgtta tcgtaaaaga ggaataggta aagtggtaat ggaaaaattg gcgtcattta
     7621 tcacttcaac atttcaggat attaatgaga ttgtgttaac ggttaatact gacaatccac
     7681 atgccatggc actttatcgc caacaaggat atcaatatat gggagatagt atgttcgtcg
     7741 gaagacctgt tcatattatg gcgctaacta taaaataaat taaatttaaa agcatcttta
     7801 ctcatcgtcg accacaacaa ttaatgatga ataaaggtgc tttttgttat agaccaccgg
     7861 acaatttact atagtaaaaa gcgacctagg gaacaattga catatatcca caggtcgctt
     7921 accttaagtt atattgctag ttgcgattaa ttgatagact catcattttt gcgctgtcga
     7981 gatggtcttt ttattaaaaa tgccgtaatc caagccgtaa tcggaatact gattgcaacg
     8041 gcaataccgc ctaaaataat agaaataaat tcttgggcaa atattttcga gtttataata
     8101 tgaccaaatg aatatttaag tttgaaaaac caaataaata aagcaagttg gccaccaaaa
     8161 aaggcaaggt aaatcgtgtt cgcagatgtc gctaaaattt ctctaccaac acgcatgcca
     8221 gattggaata attcgtattg cgtaagtgtt ggattcactt gatgcaattc ataaatgggt
     8281 gaactaatgg taatcgttaa atctatcaca gctgcaataa cagcaagaat aatagtgaac
     8341 accataaatt gaatcatatc aatgccaata ttcattgaat acacatatgt ttcatcttgt
     8401 tgttcggttg aaaagccttg tagatgaccg aagtagaccg ataaataaat gagtgtaatc
     8461 aacaatattg ttgtaacgat agtgctgata aatgcagctt gtgttttaac attgtaacta
     8521 ttgagtacga ataaattaca agcgccaata ataatgcaga aaaagaatgt gacgacataa
     8581 atcggtacgc caaaaataat caatacaata ctaataatta aaatagcgaa atttaaaaat
     8641 agggttaaat aagagatgaa tcccttttta cctccgaaaa ttatcatcag aaagaggagc
     8701 aataacgcca atataaatac agcattcatt gtttcgccct ccttaatgtt tcaaatattt
     8761 ccataaacaa tattgtgata ggaattgtaa gtacgatacc tataccgcct gttagtgcgc
     8821 gcgcaatttc taacgaccaa ttcatcgaaa tagtataagt cactgtattg gcatttttta
     8881 aaaagattaa aaacataggt agtgcaccgg ataaatatga gaataataag atgttagtca
     8941 ttgttcccat aatatcttgg cctatgtttc gcccagcaag cgcccatctc ctcattgaaa
     9001 tgtgcggcgt acgctgtaaa atttcatgca taccactagc aattgtaatt gcaacatcca
     9061 taatagcgcc aagtgaacct attaacactg aggctaggaa gatatctttc ggtggtaatg
     9121 ataaaaagtt catcgtttca tatttaatgc ctttaccatc tgtcatatat atgattaatt
     9181 ctgttaaacc tatactcaaa aaagttccga taattgtact ggctatggta atgagtgtac
     9241 gcatatgcca gcctgtaacg agcaataaag tgagtattgt tgaacagatc atggcaatgg
     9301 tcatgagtaa gaataaatta atattgctat gttgaatatg aatgtaaatt gcgattaata
     9361 tggcaataga attcaagatt aatgataaaa tcgattgcag tccgactttg cgaccaacca
     9421 ataatacagt taataagaac aaaccagtga tgataaccgt taaggtatca cgcttctttt
     9481 ctataatata agcatcactc ggcttgttag aaatatgtaa taatactttt tcgtgtgtgc
     9541 gaaatgcctc agaatctgct tgcgatttga cgtactgatg attaatcgtc gtcgtttctc
     9601 cagcaaattg accatttaat attttgactt ttaattgatt tttatattta atatcacgat
     9661 tattttgtgc atcttttgta ggtgtcgaag aaacatgttt gacatctata atttgaccaa
     9721 ttggtttgtt gtaaaagttc tcattattga atgtgaataa aatagcacca atgaatgcga
     9781 tgcagaacaa acctaaaatt atattaaatg gctttgtaaa taaatttcta tatttcaaaa
     9841 acaaaacccc aattctatga atgaattaat atggtgatta tacgccctta attttttatt
     9901 ttcaaagata ttactgtgaa atgtataacg aaaatcatca ttgatagagt cgaatggctt
     9961 aatggaatgt agacgtttta gtcattaatt gttgaatgag tgttaataag ataccaatat
    10021 cactcttcgt ataaggttct ttcgtaatag cacatatcgt tctttttaat tcagtatggt
    10081 ctaattttat atctatacat gatttagatt ctggtaaatg tatattttgt gatgaaatga
    10141 tgtaaccttc tttttgacga aggagatact gcgcaagtgg ttggctactg attgtgtata
    10201 catctgattt agtaatctta cgcaattgtt tttttacaga ttcggcaaat ggtcccaagc
    10261 aataaatatg actatgctca aactgaatta atggtgggtg tgtcgccatc gtgattggat
    10321 cgtctgaagg cgcatataaa tgatagtgct cttcgaataa aggtagcata tgtaattgtt
    10381 tgtgtttacg tatttctggt gtaagttccg tgaaaccaat gtctatattt ccatttaata
    10441 cgctatttat aattgtgtca tgttctaata aattagggat gacatgtgta tcattttgta
    10501 aatgaaacgt ttggataagt ggtagtaaca tgtgggatac gtcactctca ttatagccaa
    10561 tgtagatgct tttattttta gttaatccat gactttgaaa ttgttcaatc gtgctatcta
    10621 aatgttcaat aatacgcaga gcttcattaa ataataattt cccttcagat gtgagcgtaa
    10681 tattacgtcc ttgcttttta aataatgaca cattaagttc ttgttctaat aatgtaattt
    10741 gacggcttat cgctgattga gcaatgttta gttcaagtgc tgtttcggag atatgttctc
    10801 ttttagcgac ctcgataaaa tatcttaatt gtttaatttc catagcgata taggcacctc
    10861 caaaaatgag tgttttgtaa ctattatagc aatattattg ataaatgttc tattttttag
    10921 atgaatatct tctattttat atattgaaca gataaatttt ttagattata gtaattatca
    10981 ttaataacta atatcagaat attctaaaaa aggggtgtgc atcatgcaca atgagaaatt
    11041 aattaaaggc ttatatgact atcgtgagga acatgatgcg tgtggtattg gtttttatgc
    11101 gaatatggac aataaaaggt ctcacgacat cattgataaa tcgcttgaaa tgttgcgacg
    11161 tttagatcac aggggcgggg tcggcgcaga tggcatcact ggtgatggcg caggtattat
    11221 gactgaaata ccttttgcat ttttcaaaca acatgtaacg gactttgata tcccaggtga
    11281 aggtgaatat gccgtggggt tatttttttc caaagaacgc attttaggtt ctgaacatga
    11341 agtagttttt aaaaaatatt ttgaaggcga agggttatca attcttggtt atcgtaatgt
    11401 accagttaat aaagatgcca ttgctaaaca tgtagcagat acgatgccag tcattcaaca
    11461 agtgtttatt gatattaggg acattgaaga tgttgaaaag catttgtttt tagcgagaaa
    11521 acaattagag ttctattcga ctcagtgcga tttagaattg tattttacga gcttatcacg
    11581 caaaacaatt gtatataaag gttggttacg atcagaccaa attaaaaaat tctatacaga
    11641 tttatcggat gatttatatc aatcaaagct agggttagtg cattcgagat ttagtacgaa
    11701 tacattcccg agttggaaaa gggcgcatcc taaccgtatg ttaatgcata atggtgagat
    11761 taacacgatt aaaggtaatg tgaactggat gcgagcacgt caacataaat taatcgaaac
    11821 attatttggt gaggatcaac ataaagtgtt tcaaattgtc gatgaggatg gtagtgactc
    11881 tgctattgta gataatgcgc tagagttctt atcgttagcc atggagccag aaaaggcagc
    11941 gatgctactc atacctgaac cttggttata taatgaagcg aatgatgcaa atgtacgtgc
    12001 gttttatgaa ttttatagtt atttaatgga gccgtgggat ggtcctacaa tgatttcgtt
    12061 ctgtaacggt gacaaacttg gcgcgcttac agatagaaat ggattacgtc caggtcgtta
    12121 tacgattact aaagataact ttattgtctt ttcatctgaa gtgggtgttg tggacgtacc
    12181 tgaaagtaat gttgctttta aaggtcaatt gaatcctgga aagttattgc ttgttgattt
    12241 taaacagaat aaagtcattg aaaataatga tttaaaaggt gcgattgccg gagaattacc
    12301 atataaagcg tggattgata accataaagt tgattttgat tttgaaaata tacagtatca
    12361 agattcgcaa tggaaagatg agacgttatt taaattacaa cgtcaatttg catacacgaa
    12421 agaagagatt cataagtata ttcaggaact tgtagaaggt aagaaggatc ctatcggtgc
    12481 aatgggatat gatgcgccaa ttgcagtgtt gaacgagcga ccagaatcac tatttaatta
    12541 ctttaaacag ctgtttgcac aagttacgaa tccaccaatt gatgcgtatc gtgaaaaaat
    12601 cgtaacgagt gaactttctt atttaggtgg cgaaggtaac ttactagcac ctgacgaaac
    12661 ggttttagat cgtattcaat tgaaaagacc ggtattgaat gaatcacact tagcagcgat
    12721 tgatcaggag cattttaaat taacatattt atcaacggta tatgaaggga atttggaaga
    12781 tgcgttagaa gtattaggcc gagaagcagt ggatgctgta aagcaaggcg ctcaaattct
    12841 agtgttagat gatagtggat tagttgatgg caatggcttt gcaatgccga tgttactcgc
    12901 aataagtcat gtgcatcaat tacttattaa agcagattta cgtatgtcta caagtttagt
    12961 cgctaaatct ggtgagacac gagaagtgca tcatgttgct tgtttactcg catatggagc
    13021 gaatgcaatt gtgccatacc tagcgcaacg tacagttgaa caactgacat tgacagaagg
    13081 gttacaaggc accgttgtcg ataatgttaa gacatatacg gatgtattgt cggaaggtgt
    13141 tattaaagta atggcgaaga tgggaatttc gacagtgcaa agttatcaag gtgcacaaat
    13201 atttgaagcg attggcttgt ctcatgatgt gattgatcgt tattttactg gaacacagtc
    13261 taagttatct ggtatttcga ttgatcaaat tgatgctgaa aataaagaac gtcaacaaag
    13321 tgatgataat tatcttgcat caggtagtac attccaatgg agacaacaag gtcaacatca
    13381 tgcttttaat ccggaatcta ttttcttatt gcagcacgca tgtaaagaaa atgactatgc
    13441 gcaatttaaa gcatactctg aagcggtaaa caaaaataga acagatcaca ttagacattt
    13501 acttgaattt aaagcatgta caccgattga catcgaccaa gttgaaccgg taagtgacat
    13561 tgtcaaacgt tttaatacag gggcgatgag ttatggatcg atttcagcgg aagcacatga
    13621 aacgttagca caagccatga accaattagg tggaaagagt aatagtggtg aaggtggcga
    13681 agatgcaaaa cgttatgaag tacaagttga tggaagcaac aaagtaagtg cgattaaaca
    13741 agttgcttct ggacgttttg gtgtaactag tgattattta caacatgcca aagaaattca
    13801 aattaaagtt gcgcaaggtg caaagcctgg tgaaggtggt caattacctg gtactaaggt
    13861 atatccgtgg attgcgaaga caagagggtc aacgccaggt atcggtctga tttcaccacc
    13921 gccacatcat gatatttatt caatagaaga tttagcgcaa ctgatacatg atttgaaaaa
    13981 tgcgaataaa gatgcagata tcgcggtaaa attagtttcg aaaacaggtg ttggtaccat
    14041 tgcatctggg gtggcgaaag catttgcaga taaaattgtc atcagtggtt acgatggtgg
    14101 tacaggggct tcacctaaaa cgagtataca gcatgccggt gttccttggg agattggttt
    14161 agcagaaaca catcaaacat taaaactaaa tgacttaaga agtcgtgtta agttagaaac
    14221 agacggtaag ttattaactg gtaaagatgt agcgtacgca tgtgcgcttg gagcggaaga
    14281 atttggattt gcaactgcac cattagtggt gttgggctgt attatgatgc gtgtatgcca
    14341 taaagataca tgtccagtag gagttgcaac tcaaaacaaa gatttacgtg ctttatatag
    14401 aggtaaagca catcatgttg ttaattttat gcattttatt gcacaagaat taagagaaat
    14461 tttagcatct ttaggtttga aacgtgtaga agacttagtt ggaagaactg atttattaca
    14521 acgatcatca acattaaaag cgaatagcaa agcggctagt attgatgttg aaaaactgtt
    14581 atgtcctttc gatgggccaa acacaaaaga aattcaacaa aatcataatc ttgagcatgg
    14641 atttgattta acaaatttat atgaggtaac gaagccatat attgctgaag ggcgtcgcta
    14701 tacaggtagc tttacagtaa ataatgaaca acgtgatgta ggggttatta caggtagtga
    14761 gatttcgaaa caatatggag aagcaggact tcctgaaaat acaattaatg tttatacgaa
    14821 tggtcatgct ggtcaaagtc ttgcagcata tgcaccgaaa ggcttaatga ttcatcatac
    14881 tggagatgcg aatgactatg ttggtaaagg attatctggt ggtacggtca ttgtcaaagc
    14941 accttttgaa gaacgacaaa atgaaattat tgctggtaac gtctcattct atggtgcgac
    15001 aggtggtaag gcatttatta acggtagtgc aggagaaaga ttctgtatta gaaatagtgg
    15061 tgtagatgtt gtcgttgaag gtatcggcga ccatggatta gagtacatga ctggtggaca
    15121 tgtcattaat ttaggtgatg taggtaagaa cttcggtcaa ggtatgagtg gtggtattgc
    15181 ttacgttatc ccgtctgatg tagaagcttt tgttgaaaat aatcaactag atacgctttc
    15241 gtttacaaag attaaacacc aagaagaaaa agcattcatt aagcaaatgc tggaagaaca
    15301 tgtgtcacat acgaatagta cgagagcgat tcatgtgtta aaacactttg atcgcattga
    15361 agatgtcgtc gttaaagtta ttcctaaaga ttatcaatta atgatgcaaa aaattcattt
    15421 gcacaaatca ttacatgaca atgaagatga agcgatgtta gctgcatttt acgatgacag
    15481 taaaacaatc gacgctaaac ataaaccagc cgttgtgtat taaggaaagg gggagatacg
    15541 atgggtgaat ttaaaggatt tatgaagtat gacaaacagt acttaggtga attatcactg
    15601 gtagaccgtt tgaagcatca taaagcatat caacaacgat ttactaaaga agatgcctct
    15661 atccaaggtg cgcgatgtat ggattgtgga acgccgtttt gtcaaaccgg acaacagtat
    15721 ggtagggaaa caataggttg tccaattgga aactacattc ctgaatggaa cgactttgtg
    15781 tatcatcaag attttaaaac tgcttatgaa cgcttaagcg aaacaaataa ctttcctgac
    15841 tttacagggc gtgtatgtcc tgcaccatgc gaaagtgctt gtgtgatgaa gattaataga
    15901 gaatcgattg cgattaaagg tattgaacgc acaattattg atgaagcttt tgaaaatggt
    15961 tgggtagcgc cgaaagttcc gagtcgccgt agagatgaaa aagtggcaat cgttggaagc
    16021 ggtccagcag gattaactgc tgctgaagaa cttaatctac taggatatca agtgactatt
    16081 tatgaacgtg ctagagaatc aggcggttta ttaatgtatg gtatacctaa tatgaagctt
    16141 gataaagatg tggttcgacg tcgtattaag ttaatggaag aagcgggcat tactttcgtt
    16201 aatggtgttg aagtaggcgt tgatattgat aaagcaacgt tagaatctga gtatgatgcc
    16261 attatattat gtactggtgc acaaaaaggt agagatttac ctttagaagg acgcatgggt
    16321 gatggtatac atttcgctat ggattattta actgaacaaa cgcaactgtt aaatggagaa
    16381 attgatgata taacaataac tgcaaaagat aagaatgtca ttatcattgg tgctggtgat
    16441 acaggggcag actgtgtagc gacagcatta agagaaaatt gtaagtcgat tgttcaattt
    16501 aataaatata cgaaattgcc agaagcaatt acgtttacag aaaatgcatc atggccttta
    16561 gcaatgccgg tgtttaaaat ggactatgcg caccaagagt acgaagctaa gtttggtaag
    16621 gaaccacgtg catatggtgt tcaaacaatg cgttacgatg ttgacgataa aggacatata
    16681 cgtggtttgt atactcaagt tttagagcaa ggcgaaaatg gtatggtcat gaaagaagga
    16741 cctgaaagat tttggcctgc tgaccttgta ttattatcaa tcggcttcga aggtacagaa
    16801 ccaacagtac cgaatgcttt taacattaaa acggatagaa atcgaatcgt ggctgatgat
    16861 acaaactatc aaactaataa tgaaaaagta tttgctgctg gagatgctag acgtggtcaa
    16921 agtttagttg tatgggcgat taaagaaggt agaggcgtag cgaaagcagt agatcagtat
    16981 ttagcgagta aagtttgtgt ataatctttg tgtgacaatg atgatgttcg cgttgacgtt
    17041 gtatggtgct gaattgaaaa ggaagcgact tagtatctat caacgtcaca tgccatcttt
    17101 gtaacctaaa aataaaggtt tgtaagacaa ctaatagatt aattataagt agtgattttt
    17161 tacattcgtt ttataggtca actgtagtgg aagacaatga tttgtggtaa tcatgtaatg
    17221 cttaaaaaca atattgactt ttacagaacg ttcatatatg ataaatattg tgttt
//
LOCUS       DAMZPP010000054        17114 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMZPP010000054 DAMZPP010000000
VERSION     DAMZPP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17114
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.054"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..1080
                     /locus_tag="POH73_001333"
     CDS             <1..1080
                     /locus_tag="POH73_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6887296.1"
                     /translation="PLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVYRQ
                     QDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTMAD
                     IQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPNDN
                     IFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQGS
                     EFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDSEAFDIGLKTNGQARLTQLC
                     TLLKNYFNSEDEDMLENTATNDTGASQTTIDDQVEAPMSSNASEEVTSDSTKTDTNVL
                     TEATMFKIDPMINMGEITPYDFIER"
     gene            1423..4053
                     /gene="alaS"
                     /locus_tag="POH73_001334"
     CDS             1423..4053
                     /gene="alaS"
                     /locus_tag="POH73_001334"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDI6887297.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAVSGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIVSAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDNQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            4116..4376
                     /locus_tag="POH73_001335"
     CDS             4116..4376
                     /locus_tag="POH73_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDI6887298.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            4380..4808
                     /gene="ruvX"
                     /locus_tag="POH73_001336"
     CDS             4380..4808
                     /gene="ruvX"
                     /locus_tag="POH73_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDI6887299.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            4823..5131
                     /locus_tag="POH73_001337"
     CDS             4823..5131
                     /locus_tag="POH73_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDI6887300.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            5416..6054
                     /locus_tag="POH73_001338"
     CDS             5416..6054
                     /locus_tag="POH73_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDI6887301.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLVTHHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            6057..6980
                     /locus_tag="POH73_001339"
     CDS             6057..6980
                     /locus_tag="POH73_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDI6887302.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            6992..8260
                     /locus_tag="POH73_001340"
     CDS             6992..8260
                     /locus_tag="POH73_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDI6887303.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            8260..8883
                     /gene="udk"
                     /locus_tag="POH73_001341"
     CDS             8260..8883
                     /gene="udk"
                     /locus_tag="POH73_001341"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDI6887304.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            8911..9387
                     /gene="greA"
                     /locus_tag="POH73_001342"
     CDS             8911..9387
                     /gene="greA"
                     /locus_tag="POH73_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDI6887305.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            9713..10447
                     /gene="pxpB"
                     /locus_tag="POH73_001343"
     CDS             9713..10447
                     /gene="pxpB"
                     /locus_tag="POH73_001343"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDI6887306.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITPSKLIENLELNKFNEKNVHAVNQTNSIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     EFIQIERDISDGNFNVDDWVVIENVN"
     gene            10437..11447
                     /locus_tag="POH73_001344"
     CDS             10437..11447
                     /locus_tag="POH73_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDI6887307.1"
                     /translation="MSIKILQPGLFSTVQDLGRKGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSVNASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHTINVPPIAESYATHTRSSIGGFKGRKLLANDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            11449..11898
                     /locus_tag="POH73_001345"
     CDS             11449..11898
                     /locus_tag="POH73_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HDI6887308.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKVNDNSDVSTNDYHDIKSPMVGTFFLQDSKELTEPIVKVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            11912..13273
                     /locus_tag="POH73_001346"
     CDS             11912..13273
                     /locus_tag="POH73_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDI6887309.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNDAVNHVSEIEKLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDNAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQNDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLRATLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            13273..14025
                     /gene="pxpA"
                     /locus_tag="POH73_001347"
     CDS             13273..14025
                     /gene="pxpA"
                     /locus_tag="POH73_001347"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDI6887310.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNEEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIASVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            14037..15269
                     /locus_tag="POH73_001348"
     CDS             14037..15269
                     /locus_tag="POH73_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDI6887311.1"
                     /translation="MGRNLKLKHRESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQT
                     AVFTSQFLASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISIL
                     IAFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMI
                     LGIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAH
                     RILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVF
                     EHAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLKERSNLIVIVFIVIST
                     MIFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAV
                     LVTILTGIFSFKEVLQLF"
     gene            15559..16778
                     /locus_tag="POH73_001349"
                     /pseudo
     CDS             15559..16778
                     /locus_tag="POH73_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
BASE COUNT         6226 a         2267 c         3352 g         5269 t
ORIGIN      
        1 cctttagatg cacaattgat atttgtaggt gatgaggacc aattgccttc tgtaggtcct
       61 ggtcaagtat ttaaagattt aattgaatcg aaagctatac cacgagttaa tctgactgaa
      121 gtatatcgtc aacaagatgg ttcaagcatt atagaattag cacatcgaat gaagttgggt
      181 cagaaaattg atattacaca gcgttttcat gatagaagtt ttattaactg tcaggcaaac
      241 caaataccaa cagttgttga gaaggtagtt actagtgctg ttaataaagg gtatactatg
      301 gctgatattc aagtgcttgc accaatgtat aaaggaaatg ccggtattaa acgtttaaat
      361 caagttttac aagatatttt aaaccctaaa aagaaagaca cacgagaaat tgagtttggt
      421 gatgtagtct ttagaaaagg tgacaaagta ttacaacttg ttaataggcc aaatgataac
      481 atattcaatg gtgacatagg agttattgtt ggtatttttt gggcgaaaga aaatgccctt
      541 aataaagatg tgcttgttgt ggattttgaa ggtaatgaaa ttacatttac gaaacaagat
      601 atgatggaat taacacatgc atattgtaca tcaatccata aatcacaagg ttcagaattt
      661 cctattgtta tcatgcctat tgttaaacag tattttagaa tgttacaaag accgatatta
      721 tatactggtt taactagagc gaaaaccagt ttagttttac ttggtgattc agaagcattt
      781 gatattggat tgaaaacaaa tggtcaagcg cgtttaacac aattatgtac attgttgaaa
      841 aattatttta attcagaaga tgaagacatg ctggaaaata cagcaactaa tgatacagga
      901 gcttcgcaaa cgacaataga tgatcaagta gaagcgccaa tgtcatcgaa tgctagtgaa
      961 gaagttacgt ctgattcaac aaaaacagat acaaatgtat taacggaagc tacaatgttt
     1021 aaaattgacc caatgattaa tatgggagaa atcacgccgt atgactttat agaacgttga
     1081 caacaagcgt aaatgtaact aaaatagaat taaattcgta taaagatgag tagttaatga
     1141 tttgtttatc agagatgtac tggttggtga aaagtacaac aaacattagt gaacgctact
     1201 ttatgcacaa ctgtatagtc gatttaaagt gataaatgta ttgaattgca tttataacta
     1261 gggtggtacc gcgataacgt tcgtcccttt tgaggatggg cgttttttat tttttaataa
     1321 taaaaagtga tattgattta tactagtttt tcaaatcgac tatctgtgtc atcaattttt
     1381 atggaatggt aatttaaaaa cagtaaatag gatgtgtaaa tgatgaaaaa gttaaaagcg
     1441 agtgaaatta gacaaaaata tctagatttc tttgttgaaa aaggacatat ggttgaacct
     1501 tctgcaccat tagtgccaat tgatgatgat acattattat ggattaattc aggtgtagca
     1561 acattaaaga aatattttga tggacgtgaa acacctaaaa agccaagaat tgtaaactct
     1621 caaaaagcta ttcgtacaaa tgatattgaa aatgttggtt tcacagcgcg tcaccataca
     1681 ttctttgaaa tgttaggtaa cttctcaatt ggtgattact ttaaacaaga agcgattgaa
     1741 tttgcttggg aatttttaac gagtgataaa tggatgggta tggagccaga taaattgtac
     1801 gttacgattc atccggaaga tatggaagca tacaacattt ggcataaaga tattgggctt
     1861 gaagaaagtc gtattattcg cattgaaggt aacttctggg atattggtga aggaccttca
     1921 ggaccgaaca ctgagatttt ctatgatcgc ggagaagcat atggacaaga cgatccggca
     1981 gaagaaatgt atccaggtgg agaaaatgaa cgctatcttg aagtatggaa cttagtattt
     2041 agtgaattca atcataataa agatcatagt tacacaccat tacctaataa aaacattgat
     2101 accggcatgg ggcttgagcg tatggcctca gtttctcaaa atgtacgtac taactatgaa
     2161 acagatttat ttatgcctat aatgaatgaa atcgaaaaag tatcaggtaa acaatattta
     2221 gtaaacaacg aacaagatgt ggcatttaaa gtaattgctg accacattcg tacgattgca
     2281 tttgcaattt ctgatggtgc attacctgcc aatgaaggta gaggatatgt attacgtcga
     2341 ttgttacgtc gtgccgttcg ttttagtcaa acgttaggaa tcaatgagcc atttatgtac
     2401 aaacttgttg atattgttgc agacattatg gaaccatatt atccaaatgt taaggaaaaa
     2461 gcagatttca ttaagcgtgt tataaagtct gaagaagaac gattccatga aacattagaa
     2521 gatggtttag cgattttaaa tgaattaatt aaaaaagcta aagcgacaac aaatgaaatt
     2581 aatgggaaag atgcatttaa attgtatgat acgtacgggt tcccaattga attaactgaa
     2641 gaaatagcag tgcaagcagg attgaaagtt gatatgacaa cattcgagtc agaaatgcaa
     2701 caacaacgtg atcgtgcacg tcaagcacgt caaaattctc aatcaatgca agttcaaagt
     2761 gaagtattga aaaatattac atctgcaagt acttttgttg gttatgatac tgcgacagct
     2821 caaacgacac taacacactt gatatataat ggtgaagaag tttcacaagt tgaagcgggt
     2881 gaaacagtat acttcatgtt aacggaaaca ccattttatg cagtcagtgg tggacaagtt
     2941 gcggatacag gtattgtata taatgacaat tttgaaattg ctgttagtga agtaaccaaa
     3001 gcgccaaatg gtcaaaactt gcataaagga gtagtacaat ttggccaagt aaatgttggc
     3061 gctacagtgt ctgctgaagt gaaccaaaat gatcgacgtg acattcaaaa gaaccatagt
     3121 gcaacacatt tattacatgc agcgttgaaa tcagtactgg gtgatcatgt taaccaagct
     3181 ggttcattag tagaagcaga tcgtttacgt tttgatttct ctcattttgg tccaatgact
     3241 aatgatgaaa ttgatcaagt tgaacgctta gtaaatgaag aaatttggaa aggtattgac
     3301 gttaacattc aagaaatgga tattgtttca gctaaagaaa tgggcgcaat ggcattattc
     3361 ggtgaaaaat atggtgatgt tgtgcgtgta gtaaatatgg caccattttc aattgaatta
     3421 tgtggtggta ttcatgtccg caatacttct gaaattggct tattcaaaat agtaagtgag
     3481 tcaggtacag gagctggtgt gcgtcgtatt gaagcattaa caggtaaagc agctttctta
     3541 tatttagaag atattcaaga gaaatttaat acgatgaaat cacagctgaa agtgaaatct
     3601 gataatcaag tagtcgataa gttaacacaa ttgcaagatg aagaaaaagc attattaaaa
     3661 caattagagc aacgtgacaa agaaatcaca tcacttaaaa tgggtaatat tgaagatcaa
     3721 gttgaagaaa tcaatggcta taaagtattg gttactgaag tggatgtacc aaatgcgaaa
     3781 gcaattcgct cgacaatgga cgattttaaa tctaaactac aagatacaat tatcattctt
     3841 gcaagtaatg ttgatgataa agtatcgatg gttgcaactg tccctaaatc tttaacaaat
     3901 aatgttaaag ccggtgatct tatcaaacaa atggcaccaa tcgttggtgg taaaggtggc
     3961 ggtcgtccag atatggctca aggtggcggt acacaacctg aaaatatctc aaaatcatta
     4021 agctttatta aagattacat taaaaatcta taatttaaag ccataatatt gtatgatgat
     4081 agtgagttat atttgtaatt aaaggagtgt cgcaaatgga aaactttgat aaaacaatga
     4141 aattcgacta tgaagaactt ccaacacaag atgtaagaga tgttttaaat aatgtttatc
     4201 gaacattaga tgaacgagga tataatgccg taaaccaaat tgtaggttat ttattatcag
     4261 gtgaccctgc gtatattcca cgccaaaatg aagcaagaaa tcaaattcgt catattgata
     4321 gagatgttat tatggaagag cttgtttctt actatttaaa agagcaaaat aaataattta
     4381 tgttacaaca taaaatttta ggactcgatg tcggtagtag aacggtagga attgcaatta
     4441 gtgatataat gggttggacg gcacaaggat tagacacact ccgaatcaat gaagaaaata
     4501 atgaattagg tattgaccaa ttagtagaca ttattaaaaa acataatgtg ggtacagtcg
     4561 taataggact acctaaaaac atgaataatt caataggatt tcgaggcgaa gcttcgttaa
     4621 catataaaga aaaattatta gaagcttatc cttctattga gattgtgatg tgggatgaaa
     4681 gattaagcac aatggctgct gaacgatcat tattagaagc agatgtttca agacaaaaaa
     4741 gaaaacaagt gattgataaa atggcagcgg tatttatttt acaaggctat ttagattcac
     4801 tacattaaaa ggagatttta ctatgactga acataatcat gattcacaac tagaaattaa
     4861 taacgaagaa gaattattaa ctttattcga tgaagaggga aatgaagttt tataccgaaa
     4921 agttttagaa ttttatcatc ctgaattcaa aaaagagtat gttatcttag ctgaagaagg
     4981 tgctcaatca gatgaagacg atatgattga gcttgtacca atgatcaatg aaccagatga
     5041 gtcaggtgac ggtggtaagt tagtaccaat cgaaactgat gaagaatggg acatgattga
     5101 agaagttgta aatactgaaa tggaagaata attaaaaaga ttttactctt gctaacctta
     5161 agatggggag gcaagagtat ttttttaagt caaacaatgt ataaataatt aacgcttata
     5221 attttattga aaatatggta aaatatctaa agttaaattt tatatgaatg attcgcttct
     5281 ataaataaaa tagctttgcg tctaacacga aatcagtcat ttaattggtt ggcaaaatta
     5341 taatcaattg tgaaggctga tttattttat tgtcataatc atattgactt attttacata
     5401 aaggaagttt aaaacatgga tgacctaaat aaaaaatatt taatagattt acatcaacat
     5461 caaaatagtt caatcgaagt tttgcgtgaa tttgccgaag taaatgaagt gccaatcgtg
     5521 gatcgtttaa cattagattt aattaagcaa ttaattcgta tgaataatgt taaaaatatt
     5581 ttagaaattg gtacagcaat cggctatagt tctatgcaat tcgcttctat atctgatgac
     5641 attcatgtca caacgataga gcgtaatgaa acgatgattc aatatgctaa acaaaattta
     5701 gttactcatc attttgaaaa ccaggttcga attattgaag gtaatgcttt agagcaattt
     5761 gaaaatgtaa atgacaaagt ttatgatatg atatttattg atgcagcaaa agcgcaatca
     5821 aagaaatttt ttgaaatata tacaccactt ttaaagcacc aaggtctcgt aattacagat
     5881 aatgttttat atcacggttt tgtatcggat attgggattg ttagatcgag aaatgtaaga
     5941 caaatggtta aaaaggttca agattataat gagtggttaa taaagcaacc aggatataca
     6001 acgaattttt taaatataga cgatggatta gcgatttcaa ttaaaggaga atgactatga
     6061 cagaattact tgtcacaccc aagtcattaa gccatatgga aacacttatt gatttaggtg
     6121 cagatgcttt tgtaattggc gagcaaaagt ttggtttaag acttccaggg gaattcaata
     6181 gacaacaaat gacagaggct gtcgcattag ctcacaaaaa cgataaaaaa gtatatgcgg
     6241 cggtgaatgg attatttcat aattatcatt tagatgctgt agaagattat attaattttt
     6301 tacatgaaat acgagttgac agaattattt tcggggatcc agcagtagta atgtatgtga
     6361 aggcgcaaga aaatccaata ccacttcatt ggaatgcaga gacgttagta actaatcatt
     6421 ttcaatgtaa ttactggggt aaaagaggcg catcaagagc tgtcttagca cgtgagctga
     6481 atttggatga aattattaat ataaaagaaa attcaaatgt ggaaattgag gtgcaagttc
     6541 aaggaatgac ttgtatgttc caatcaaagc gtatgctact aggcaattat tatacgttcc
     6601 aagatcgtca aatgaaaatt gaacgtcgca atgatgaaca atctttattg ctttatgatg
     6661 aagaaagaca aaataattac ccagtttacg aagattacaa tggtacgcac attatgtcac
     6721 caaacgacat ttgtttaatt gaagaattag caccattttt tgaggcgggt atagattcat
     6781 ttaaaatcga tggtattcta caaacggaag aatatattaa tgtggtaacg gaacagtatc
     6841 gtcaagcgat agatttgtac aatgaagatc ctgaaatcta tgaagatgag aaatttatgt
     6901 tgatggaccc aattgaagaa attcaacctg atcatcgacc atttgacgaa ggtttcttat
     6961 ataaacaaac agtatattaa ggaggttaat catgaagaca atagaagaga ttaaatcaac
     7021 tcctaaaaca gttatgaaga aaccagaatt attagcacct gctggaaact tagaaaagtt
     7081 aaaaatagca gtacattatg gcgctgatgc cgtattttta ggtggtcaag aatatggatt
     7141 acgttcaaat gctgataatt tcacgatgga agaaatagct gaaggtgttg aatttgcgaa
     7201 ccgttacggt gccaaaattt atgttacgac aaatattatt gcacatgatg agaatattga
     7261 aggtctagaa tcatatttgc gtaatttgga aaagactggt gcgacaggta tcattgttgc
     7321 agatccttta attatcgaaa catgtaaaga agttgcgcca aaacttgaaa ttcatttatc
     7381 tactcaacaa tcactttcta attacaaagc tgtagaatat tggaaagaag aaggattgga
     7441 tcgtgttgta ttagcacgtg agaccggcgc gatggaaatg cgtgaaatga aggaaaaagt
     7501 agatattgaa atcgaagcat ttattcatgg tgctatgtgt atcgcctatt caggtagatg
     7561 tacattaagt aatcatatga ctgcaaggga ttccaacaga ggcggttgct gtcaaagttg
     7621 tcgttgggat tatgaattat tagaagttga tgataatggt gaacttgatg tattttataa
     7681 tcaaggtgaa gttacaccat ttgcgatgag tcctaaagat ttaaaattaa tcgaatctat
     7741 tcctcaaatg atggatattg gtgtggactc attaaaaatt gaaggacgta tgaagtcaat
     7801 tcactatatt gcaacagttg tctcagtata tcgtaaagtc attgatgcgt atgcggcaga
     7861 tcctgacaac tttaagatta atccggaatg gttaattgag ttagataaat gtgcaaatag
     7921 agacactgca cctgcattct ttgagggaac acctggttat gaagaacaga tgtttggtca
     7981 acaacaatct aaaaaatcac catttgattt ttgtggtttg gtattagatt ataatgaaga
     8041 tacaaaaatt gcgactattc aacaacgaaa taactttaaa ccaggtcaag aaatagaatt
     8101 ctttggtcca gaaattgaaa catttacaca agtagtagaa gcaatttatg atgaagaagg
     8161 taacagctta gacgcggcgc gccatccatt acaaatcgtt caaattaaag tggatcgccc
     8221 gatatatcca aacaacatga tgagaaagga aattggctaa tgaaagctac tacaatcatt
     8281 ggcatagctg gtggatctgg ctcaggaaaa acaactgtaa ctaacgaaat tatgaaaaac
     8341 ttagaaggtc atagtgtcgc tttacttgct caagattact attataaaga tcaaaagcac
     8401 ttgactttcg acgagcgcct agaaaccaat tatgaccatc catttgcatt cgataatgat
     8461 ttattaattg aaaatcttaa agacttgaaa aatggtaaag cagtagaagt accgacatat
     8521 gattatgcta gtcatacaag aagtgacatt accattgatt ttaaacctaa agatgttatt
     8581 atcgtagaag gtattttcgc tttagaaaat aaggtattac gtgatatgat ggatgttaaa
     8641 atatatgttg atacagatgc agacttgaga atattacgcc gtttaacacg agatactaaa
     8701 gagcgtgggc gttcaatgga ctctgttatc aatcaatatt taagtgttgt tagacctatg
     8761 catgaccaat ttattgaacc gactaagaaa tatgctgata taattattcc tgaaggtggg
     8821 agcaataaag ttgcaataga tattatgaca acaaaaattc agtctttagt tagcaagcaa
     8881 tagtataatt cagtaaagga agatgacaat atggaaaatc aaaagcaata tccaatgact
     8941 caagaaggtt ttgaaaaatt agagcgtgaa cttgaagaat taaaaacagt taagcgtcct
     9001 gaagttgtag agaaaattaa agttgcacgt tcatttggtg acttatcaga gaactctgag
     9061 tatgatgcag caaaagatga acaaggattc atcgaacaag atattcaaag aattgagcat
     9121 atgttaagaa atgcattaat cattgaagat actggagata acaacgttgt taaaattggt
     9181 aaaacagtaa cgtttgtaga attaccaggt gatgaagagg aaagttatca aatcgttggt
     9241 tcagctgaat cagatgcatt taatggtaag atttcaaatg aatcaccaat ggctaaagcg
     9301 ttaattggta aaggtttaga tgatgaagtg cgtgttccac tacctaatgg tggcgaaatg
     9361 aacgtaaaaa ttgttaatat ccaataaatg atcaaataga ttgtttaatt gcgcgattga
     9421 agtgtgttaa tgatacttcg gtcgtgcttt tttatattca aaataaaaaa atcggacaga
     9481 tgaaaagtga aaattttcca tcagaccgat tgatagcaga atctaaatta gtgtaagagt
     9541 ttcaatatta actactccat tttaaaaaag caaaatattt ccgaagaaac aaattgatta
     9601 taatttgtta ttgtatgcga tttgaattac ttattaaaca acttgtgctt ctcaatgata
     9661 caatgaaaag gtattcaata ctggtttatt gcaatacaaa gggggattta gagtggatgt
     9721 aagatttatt aacgagcaga cgattatgat ttattttgaa aataaaatat ccgaagagac
     9781 atatcgaaat gtcacagcaa tggtacggtg gatacgagaa aaagaaatat tagaaattca
     9841 agatattgtc ccttcgtatc gagcagttct aatatatttt gatgaacaag ctataacacc
     9901 ttctaaatta attgaaaatt tagaacttaa caaatttaat gaaaaaaatg ttcatgctgt
     9961 gaatcaaacc aatagtatta ttaaaatacc tgttcaatat ggtggtacat atggacctga
    10021 tattgaagaa gtagcaaagc ataatcgaat aactgttgag caagttattg aaaaacatac
    10081 aagtaaacct tatttaatat atatgctagg atttatgcct ggatttccat acttaggcgg
    10141 acttgatgaa caactacata cacctagacg taatcagcca aggttaaaaa ttcatgcagg
    10201 ttctgtaggt attgcgaata atcaaacagg actatatcca tcagattctc ctggcggatg
    10261 gcaaattatt ggacgcacac cgttaaaagt atttagcagc gagcgtgaac caatgtcaat
    10321 gtatgaagca ggagaatgga tacaatttta tgcaatagat gaacaggaat ttatacaaat
    10381 cgaaagagat atatcagatg gcaactttaa tgttgatgac tgggtggtga tcgaaaatgt
    10441 caattaagat tttacaacca ggtctctttt caacggtaca agatctagga agaaaaggtt
    10501 atgaacatat tgggttttca ggcgcaggtg ccatggatca atttagtttt aaggttgcgc
    10561 agtcattaat taacaatgat ggtccagcga ttgaatatac tttgattggt cctaccattc
    10621 aatttaatac gcaaaataca tttgttataa ccggtggtag tgttaatgcc tcgctaaata
    10681 ataaaactat atcaatgaat tctgtcatat tagctgagaa aggtgacatt ttaaaaatag
    10741 gtgctataac caaaggtgca cgcggttatc ttacttttgg tcatactatc aacgtacctc
    10801 caattgctga aagttatgca acacataccc gaagtagtat aggaggattc aaaggtagaa
    10861 agttattagc taatgatgta ataacagtga aaataaataa tgactttaaa gaaaacattg
    10921 gaaagactat tcatttacag gacgatttat tgccagaaaa taatattata cacattcttc
    10981 aaggacctca attcgaggca ttttctgaag aggctagagc gaaaattgta aatcatccat
    11041 atttaattac cgaacaatca gaccgtatgg ggtatcgttt ggaaggtgac agcgttgcac
    11101 ctattaatca agcagatatc atttctgaac cggttgctct tggcagtgtt caagtaccaa
    11161 atgatggtca acctattatt cttcttaatg ataaacaaac gataggtggt tataccaaaa
    11221 ttgcaactgt atgcaaattt gatctgccaa agttagcaca gatgaaacca caagatacaa
    11281 ttcaatttaa atggatatca ttccaagaag ctgtagataa gaatcgggaa caaatgtctc
    11341 tgtttaatga aattcttaag agtcatcaaa agactccaat ttttgataca tctagcttgc
    11401 gacatacttc gaaaaaatta gcaacaattt taaaagggga tttgtaaaat gaatattgaa
    11461 aaaatcgaac aaataatcaa attagtgaag gaaaatgatg ttaagaaatt taaatataaa
    11521 aattttgaag atgaaataga aattgacttc actgattcga atcatttggc tgcacacagt
    11581 aatcaatcaa atcaaagtat gaacaataat gatttgacag cttcaaaagt gaatgataac
    11641 tccgatgttt cgacaaatga ttatcatgac attaaatcac caatggtagg tacattcttt
    11701 ttacaagaca gtaaagaatt aactgaacca attgtgaaag tcggtgacaa agttaacaag
    11761 ggagatatta taggatatgt tgaagcgatg aaagtattaa acgaggtaac aacagatgtt
    11821 gctggagaaa tcactgaaat agtagctgat catggcacaa atgttgaata cgaccaagtt
    11881 ttggtacgta ttaagtaaag ggggtaagta aatgcttcgt tgtttaattg cgaacagagg
    11941 tgaaattgct gtaaggatta taagagcttg cagagaatat gggattgaaa ctgtagcagt
    12001 ttatgcaaaa ggggacgagc aaagcttgca tgtacattta gcggatcaag ctatatgtat
    12061 tggagaagct aatgctttag atagttattt aaatattgac cgcatcatat ctgctgcaca
    12121 aatcactggt gctaatgcaa ttcacccagg atatggcttt ttatcagaat caacaaaatt
    12181 tgctcaaacc gttgaggaac aaggcatagc gtttataggg cctactaaaa aaacgatgga
    12241 aatgatgggg gataaaataa cagcaagaca aacagttcat catgcaggtg ttcctgtgat
    12301 tccaggatca aatgatgctg tgaatcatgt gtctgaaatt gagaagcttg ccaaagacat
    12361 cggttatcca gttgttatca aagctgccag tggcggtggt ggtaaaggta tacgtattgt
    12421 aaagaaagct gaagatttag aaaaggcatt taaagaagct aaaagtgaag gaaaaaaata
    12481 ctttgatgat gatcgtgttt atgttgaggc ttttatacct gttgcaaaac atgttgaagt
    12541 tcaagttatg ggggatgggc aggataatta tgtacattta ggggaacgtg attgttctgt
    12601 acaacgtaaa aatcaaaaat taatcgaaga atctccttgt gcagcattaa ctgaagaaag
    12661 acgacaacaa atatgtaacg atgctgttaa agttgcacgt gcagcaaatt atagaagtgc
    12721 aggtacgatt gaatttttag taactgataa cgcacattac tttattgaaa tgaatgctcg
    12781 aatacaagtt gaacatactg ttacagagat gcgagcagaa cgtgatttgg ttgcagcaca
    12841 attgtattta ttggaacata atcatttacc tttttcacaa aacgatattc aatttaatgg
    12901 tcatgtgata gaagcgagaa taaatgccga aaatcctgaa aagaaatttc aaccaacacc
    12961 aggtaaagtg acagcgttac atttacctca aggctttaat gtgcgagtcg attctttact
    13021 atatcacggt tatcaagttt caccatatta tgattcttta gtagctaaag taattgtaaa
    13081 atctcatgat agagcttcag caattgataa attgagagct accttagatg aaatggtgat
    13141 agatggattt tctacaactg ccgatttttt atatgcagtt ttaaattatc cactatatcg
    13201 tgatggagat gcaaaagatg ttgatattaa atttttagaa aaacatcaaa ttgttaaagg
    13261 ggtggaatta taatgcgagt tgatttgaat tgtgatttag gcgaagcatt tggaaattat
    13321 tcctttggtg gtgatcatca aattattccg ttaattacaa gtgcgaatgt tgcttgtggt
    13381 tttcacgctg gtgatgaaaa tgtaatgaat gaaacggtaa aacttgccaa agcacataat
    13441 gttgcagtag gtgcacatcc tggtttacct gatttgaaag gctttggcag acgaaatata
    13501 gatatctcta acgaggagat ttataatttg atgatttatc aattaggtgc attacaaggg
    13561 ttttgtcgca ttcatcaagt taaaattaat catgttaaac cacatggtgc attgtatcag
    13621 atgggtgcaa aagacagaga aatagcaagc gttatagcac aagctgttta tgactttgat
    13681 ccatcactag tgttagtagg attagcaaat tcatatctaa tttcagaagc aaagaatgtc
    13741 ggattaatta cagcttctga agtgtttgct gatagacgat acgaagatga tgggcagctc
    13801 gttagtagaa aagaaagtga tgctgtgatt actgatactg acgaagcact taagcaggtt
    13861 ttgaagatgg tgaaggaaaa taaagttatt tcaaaaaaca ataaggaagt aacgttacaa
    13921 gcagatacaa tttgtgtgca tggtgatgga gaacatgcat tattatttgt ttcgaaaatt
    13981 agagaaattt taatgaaaga aggcattgat attcaatcct tatagggggc taaaaaatgg
    14041 ggagaaattt aaagcttaaa catcgcgaaa gtgattttga atttactaaa aaccataaga
    14101 gattattgtt aggttctgta tttctaatgg caacctctgc aataggacca gcatttttaa
    14161 cacaaacggc agtatttaca tcgcaatttt tggcgagttt cgcatttgcc atattactgt
    14221 ctattatcat tgacattggt gcacaaatta atatatggcg catattagtt gtaactggtt
    14281 taagaggtca agaaatatca aataaagttg ttcctgggct tggtactgtt atctcaatat
    14341 tgattgcatt tggtggtcta gcttttaata ttggtaatat tgctggtgcc ggtttaggtt
    14401 taaatgcaat ttttggatta gatgtaaaat ggggcgcagc tattactgca atctttgcaa
    14461 tattaatctt tgtaagtaaa agtggacaaa aaattatgga cgttgtttca atgattcttg
    14521 gtattgtgat gattttagtt gtggcatatg tgatgtttgt ttctaatcca ccttatggtg
    14581 atgcttttgt gcatacattt gcgccagaac atccaatgaa attagtcttg ccaatcatta
    14641 cgttagttgg tggaactgta ggtggttata ttacctttgc aggtgcacat cgtatattag
    14701 actctggcat taaaggtaag caatatttac catttgtaaa tcaatcagca attgcaggta
    14761 ttttaactac aggtattatg agaacgttac ttttcctagc ggtattagga gttgttgtaa
    14821 caggtgtgac actaagttct gaaaatccac cagcgtcagt ttttgaacac gcaattggac
    14881 caattggaaa gaatattttt ggtattgtgt tatttgctgc agctatgtca tcagtaattg
    14941 gctcagcata cacaagcgca acatttttaa aaacacttca taaatcactt aaagaaagaa
    15001 gtaatttaat tgtgattgtg tttatcgtta tttcaacaat gattttctta tttattggaa
    15061 aaccaatcag ccttttaatt atagcaggcg cgataaatgg atggatttta cctattacgt
    15121 taggtgcaat tttgattgca agtaaaaaga aatcaattgt aggtgattat aaacacccaa
    15181 attggatgtt catctttggt atcgttgcag tacttgttac aatattgaca ggtatattct
    15241 cattcaaaga agtgcttcag ttattttaaa agacatttcg actaatagca tcactgtaaa
    15301 tgttgttagt attaggccga aatgtgtttt gattatcggt gattcattta tacagcataa
    15361 ttagatatat tttaagagag agtggtgcat gacaagtgta ctgctctttt tgttatgatg
    15421 aaaaatcttt ttctattgat tgagaaaata gttatttata tagtttttaa agaaaaaaag
    15481 tgataatatt tagctaatct aatgaaaatg gatttattgg actaaaaata cattttaaat
    15541 gagtagagga ggctgcaaat gggttacaga actttaaaaa gtatttttca tgaacacaat
    15601 gaaagtaaaa tgaaggaaga gtatattaag cgatttaatt ctttagcatc tttcaatact
    15661 aatattaata tcatacctat ggaaaatgga aaaaaagtta atgatttgga atatcctcta
    15721 ttctttatgg tgactaaaaa tctatcaaaa aacaagaatt aatatcaatt aatagtagaa
    15781 aaattgatag agcacttaat tctttaccat atgcagctag agaacaatat tttaatgatt
    15841 tattaatcga tgaattacaa agtactaatg aaattgaaaa tgtatttagt actaaacaag
    15901 agattgcaca tgcgttaaat aaccaagcat cagactttct taagttcagg ggactcgtgg
    15961 atcaatataa agagatagaa cttaataaaa aaattaaagt tgataatgta agagacatta
    16021 gagcgattta tgataaatta gtttcaaatg aaattaacga acaagataag ttagatggag
    16081 agctatttcg taaaaatttt gtcggtgtgc atgatgggtc aacgaataaa tatatacatg
    16141 ttgggttaca acctgaaacc aaaattgttg aatatatagg tgaaatgcta acatttttaa
    16201 aatattttga tgcgcctcag ccgttcaaaa tcatggctag tcattatatg tttgaatata
    16261 tacatccttt ttatgatggc aatggtagag ttggaagatt tataattgcc aagctcttga
    16321 gtgattatta tgataattat actgctttga cattttcata tgttataaat aggaataaat
    16381 cgaaatatta caaggcgttt atgactgctt caaatcattt gaattgtggt gatttgacgg
    16441 aatttattga cacaatgctt gagttattga tagcaggaca agaaagaata cttgatgagt
    16501 taataccaaa aatggatgcg actgaaaaat taacgcttta tttaactagt cattataaaa
    16561 agatagatta tgaatttttg tatctccttt caatggataa attgtttgga aataaaagaa
    16621 atagattaac attaattgat ttagaaaaca tattgggagt aggaagagta aaaattaata
    16681 atacaattaa aaaatttggt aactacttag ttaaaattaa aagtagacca acaatttatg
    16741 agataagtga tgaatttcta aattcgatta taaaataaat gtaatatgtg attcagctat
    16801 acttattgta tttgtaagat tgcgtaattg cttcattaaa aaatatattt gttgagagtg
    16861 gtgcatgaca agtgtactgc tctttttgtt gtgtatcagt attgtgatgt tttaattcga
    16921 atattatatg aatcattatg aaatttaata aaaataattg aaataattat cattttttct
    16981 gatatactgt taaacggttt ggaattttta agtttacact gtattggttg atataactca
    17041 actaataatt gcgaacagag tatttcaaat tgaaaagtat tatgagtgtg atacataaat
    17101 caaaattgta ggct
//
LOCUS       DAMZPP010000055        17083 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMZPP010000055 DAMZPP010000000
VERSION     DAMZPP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17083
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.055"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            301..900
                     /locus_tag="POH73_001350"
     CDS             301..900
                     /locus_tag="POH73_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HDI6887312.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLEE
                     IQARREEIKYYIAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            935..1044
                     /locus_tag="POH73_001351"
                     /pseudo
     CDS             935..1044
                     /locus_tag="POH73_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1124..1207
                     /locus_tag="POH73_001352"
     CDS             1124..1207
                     /locus_tag="POH73_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887313.1"
                     /translation="MGVCPPVTMDACALLQKFDFCNNISHF"
     gene            1570..2796
                     /locus_tag="POH73_001353"
     CDS             1570..2796
                     /locus_tag="POH73_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6887314.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMTHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAELSGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDTIFVVDRDNHLLGFLDIEDINQGIRGHKSLRETMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQAEYVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            2793..3428
                     /locus_tag="POH73_001354"
     CDS             2793..3428
                     /locus_tag="POH73_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6887315.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIILTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLILGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            3445..4386
                     /locus_tag="POH73_001355"
     CDS             3445..4386
                     /locus_tag="POH73_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6887316.1"
                     /translation="MKKIKYILVVLVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGV
                     LKEAPIKDPKKAMIATQQGFKKKFDQTFFNSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            4386..5081
                     /locus_tag="POH73_001356"
     CDS             4386..5081
                     /locus_tag="POH73_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6887317.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            5375..6775
                     /locus_tag="POH73_001357"
     CDS             5375..6775
                     /locus_tag="POH73_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6887318.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLVA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLIAVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEMWYIVSSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            7001..7936
                     /locus_tag="POH73_001358"
     CDS             7001..7936
                     /locus_tag="POH73_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDI6887319.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEAMKPHIDNETIVVC
                     TMNGLKHEEIIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            8258..9109
                     /locus_tag="POH73_001359"
     CDS             8258..9109
                     /locus_tag="POH73_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI6887320.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYKTWK
                     DGMNPIK"
     gene            complement(9216..10715)
                     /locus_tag="POH73_001360"
     CDS             complement(9216..10715)
                     /locus_tag="POH73_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDI6887321.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDDVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTNKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQNNNKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGKIILRKSKLTTFLSWLTLCLCIAIALILYALPY
                     LILGSNNWSFVLTWLPIEIKLTLTTAFIALFSMLITLLLILHTTTIKKP"
     gene            10947..12779
                     /locus_tag="POH73_001361"
     CDS             10947..12779
                     /locus_tag="POH73_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDI6887322.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSANNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     MPQTETTVLSQLAMQIFDDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PPRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGM
                     VIKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKI
                     NKHYRDIAEQLRSDIDVHNVEVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVH
                     VSFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITV
                     VIPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(12919..14997)
                     /locus_tag="POH73_001362"
     CDS             complement(12919..14997)
                     /locus_tag="POH73_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDI6887323.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAIEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITQG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKD"
     gene            complement(15135..16544)
                     /locus_tag="POH73_001363"
     CDS             complement(15135..16544)
                     /locus_tag="POH73_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6887324.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            16830..>17083
                     /locus_tag="POH73_001364"
     CDS             16830..>17083
                     /locus_tag="POH73_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDI6887325.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKNLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQ"
BASE COUNT         5753 a         2462 c         3251 g         5617 t
ORIGIN      
        1 tattgatgat gcttcccacg caccaaatag ccaaccagat cctattaaag agcctaaccc
       61 taataagact aattgggata gattgatttt ttcgttgtgc aattgcttgt ctttaatcat
      121 aaatactcct tcgttattgc cgaaaattca aaaaatagta tgcgaaaaat tatacgctta
      181 ttataataat agtcaaatgc attttttgta agaatgcaaa agcatttgta aaattagttt
      241 tttataaaat taggatatat aatacaatgt gtattgaaaa ataaaatgga agtgatgact
      301 atgagtgaga aacaatcgaa tgaacaagtt gaggcatttt taaataaaga gagtcaatgg
      361 caagaagaat ataaatattt gagagcgtta atctttaatg aaacagaatt agaggaagcg
      421 tataaatgga tgcatccttg ttacacgttg aataataaaa atgtagtact tatccatggc
      481 ttcaaaaatt atgttgcact attatttcat aaaggtgcca ttttggagga taaatatcat
      541 acactcattc aacagactga aaaggtgcaa gcagctcgtc agttacgatt tgaaaatcta
      601 gaagagattc aagcacgtcg agaagaaatt aaatactata ttgctgaagc aattaaagct
      661 gaaaaagctg gtaaaaaagt tgaaatgaag aaaacagagg aatatgttat tcctaaagag
      721 ttagaagcta agttcgaaga aatgccacag ctagagtcgt cattttataa attgacacca
      781 ggcagacagc atcaatacat ttatcacatt ggacaagcta aacgcagtga aacaagacaa
      841 aagcgtgttg aaaagtatat taaccaaata ctagaaggta aagggatgca tgataagtaa
      901 ttaatgagta aaagcatacc ggttatacaa caacatacaa gatgacacga aacaaccaat
      961 ggctcatgct gttggttgtt ttttaggtgt gcctatcatg ggcaacactt tgacggtgga
     1021 attccgttac aggcttggga gtaggaactg ttagcataag gcaagggtgt ctacaatgaa
     1081 tgatgaagat attgaaatat aaggaggact ttgtgagtgg cgggtgggcg tttgtccgcc
     1141 tgtaacaatg gatgcgtgtg cattattaca aaaattcgac ttttgcaata atatttcaca
     1201 tttttgacac ttttttgcta taaaacaacc aattgagcga taataaattc gcttttgaaa
     1261 aatatgagtt atctatttag ttgcaaaaga taaaatgata atgtttaata acatcatata
     1321 gagtatgtta gttttaaatg tcgaatatac gaatgtgcaa acaaagtaat cggtagaaat
     1381 tcaacataca tagcgccgtt tactgttaag tattcacatt acagatgaaa aatataaaat
     1441 tctacataat caagaccatg atgtgtactt gtttaactta tgactctatt tgtttaacaa
     1501 ttgcgatgat ggtcttttta ttttatgcgt atcattcgtc atatttttta tgaggaagga
     1561 gaaatgatta tgttaagtat taagcattta acgaaaatct attctggtaa taaaaaggca
     1621 gtagatgaca tctctttaga tattcagtct ggggaattta tcgcatttat tggaaccagt
     1681 ggaagtggca aaacgactgc tttaagaatg ataaaccgta tgattgaagc gacagaagga
     1741 caaattgaaa ttgatggtaa agacgttcgg agtatgaatc ctgtcgaatt acgtagaaat
     1801 attggctatg ttattcaaca aattggctta atgactcata tgacgattaa agagaatatt
     1861 gtgttggtac ccaaattgtt gaaatggact aaagaagaaa aggataaacg tgcaaaggaa
     1921 ttaattaaac ttgtggattt accggagtca tttttagagc gttatccagc agaactatca
     1981 ggtgggcaac aacaacgtat cggtgtcgta agagcacttg cggccgaaca agatattatt
     2041 ttaatggatg aaccttttgg agcattggat cctattacga gagatacgtt acaagattta
     2101 gttaaaacgt tacaacgaaa attaggcaag acgtttatct ttgtaacaca tgatatggat
     2161 gaagcgatta aattagcaga caaaatttgt attatgtcag aaggtaaggt ggtgcaattt
     2221 gatacaccgg acaatatttt aagacatccc gcaaatgatt ttgtacgtga ttttatagga
     2281 caaaatagac tgattcaaga ccgtcccaat gacaagactg tagaaggtgt aatgattaaa
     2341 ccaatcacga tacaagcaga agcaacactg aatgacgccg ttcatattat gagacaaaaa
     2401 cgtgttgata ctatttttgt agtagatagg gataaccatt tactaggatt cttagatatt
     2461 gaagatataa atcagggtat acgtggacac aaaagtttac gagaaaccat gcaacaacat
     2521 atttataccg ttcaaattga ttctaagtta caagattctg tacgtacgat tttaaaaaga
     2581 aacgttagga atgtacctgt cgtagatgat caacagcgtt tagtaggact gattacgcgt
     2641 gccaatgttg ttgatattgt atatgacacg atttggggcg atagtgagga tacagtgcaa
     2701 gcagaatatg tgggggaaga cactgcgtcc tcaaaagtgc atgagcaaca cactactaat
     2761 gtcaaagtac gtgacatagg agatgataaa tcatgatcga gttcctacat gaacatggtg
     2821 gacagttgat gtcgaaaaca ctggaacatt tctatatttc tatagtggca ttattacttg
     2881 ccatcattgt tgcagtacct ataggcattt tattatcaaa aacaaagcga actgccaata
     2941 ttatattaac tgtggcaggt gtcttacaaa ctattccaac actagctgta cttgctatta
     3001 tgataccgat ttttggtgtt ggtaaaacgc ctgcaattgt agcgctattt atttatgtat
     3061 tattacctat tttaaataac acggtactcg gcgttcaaaa tattgatagt aacattaaag
     3121 aagctggaaa aagtatggga atgacacaat ttcaattgat gaaggatgtt gaattgccgt
     3181 tagccttacc gcttatacta ggcggtattc gcttgtcgtc tgtgtatgta attagttggg
     3241 ctacacttgc aagttatgta ggtgcgggtg gattaggtga tttcattttc aatggtttaa
     3301 atttatatga tccactgatg attgtaactg caacggtact cgttactgca ctagcattag
     3361 gtgttgatgc cttattagct ttagttgaaa aatgggtagt tcccaaaggc ttaaaagtat
     3421 ctggataatt aggaggctaa gataatgaag aaaattaaat atatacttgt cgtgcttgtc
     3481 ttatcgctta ccgtattatc tggatgtagt ttgcccggac taggtagtaa gagcacgaaa
     3541 aatgatgtca aaattacagc attatcaaca agcgaatcgc aaattatttc acatatgtta
     3601 cggttgttaa tagagcatga tacacatggc aagataaagc caacattagt aaataattta
     3661 gggtcaagta cgattcaaca taatgcctta attaatgggg atgctaatat atcaggtgtt
     3721 agatataatg gcacagattt aacgggagtt ttgaaggaag caccaattaa agatcctaag
     3781 aaagcaatga tagccacaca acaaggattt aaaaagaaat ttgatcaaac atttttcaat
     3841 tcgtatggtt ttgcgaatac gtatgcattc atggtaacga aggaaactgc taaaaaatat
     3901 catttagaga cagtttcaga tttagcaaag catagtaaag atttacgttt aggtatggat
     3961 agttcatgga tgaatcgtaa aggcgatggc tatgaaggat ttaaaaaaga gtatggtttt
     4021 gactttggta cagtgagacc aatgcaaata ggtctagtct acgacgcatt aaactcagag
     4081 aagttagacg ttgcattagg ttattctaca gatggtcgaa ttgccgcgta tgatttgaaa
     4141 gtacttaaag atgataaaca atttttccca ccttatgctg cgagtgctgt tgcaacaaat
     4201 gaattattac gccaacaccc agaacttaaa acgacgatta ataagttgac aggaaagatt
     4261 tcaacttcag agatgcagcg cttgaattat gaagcggatg gtaaaggtaa agaacctgct
     4321 gtcgtcgcag aagaattttt gaaaaaacac cactattttg ataaacagaa aggtggtcat
     4381 aagtaatgga aggtaattta ttacagcaat tattcaatta ttatgttacg aactttggct
     4441 atctatggga cttatttttc aaacacttat taatgtctgt ctatggtgtg ctgtttgcag
     4501 ctttaattgg tattccattg ggaatcttgc ttgcaagata cacaaaactt tctggatttg
     4561 taattacaat tgcaaatata attcaaacag ttccagtcat tgcaatgtta gctattttaa
     4621 tgttagtcat gggcttaggc tcagaaacag tagttttaac agtgttttta tatgcgttac
     4681 ttccaattat aaaaaacact tatactggta tagctagtgt tgatgcgaat attaaggatg
     4741 ctggcaaagg tatgggaatg acacgcaatc aagtgctacg aatgattgaa ttaccgttat
     4801 ctgtttcggt tattatcggt ggcattcgta ttgccttggt tgttgcgata ggtgttgttg
     4861 ccgttggatc atttatagga gcacctacgc ttggtgacat tgtgattcgt ggtacaaatg
     4921 cgacggatgg cacaacgttt attttagcag gtgcgattcc gattgctatc attgcaatcg
     4981 tcattgatgt actattaaga tttttagaaa aacgattaga cccaacgaca cgacatcgta
     5041 aaaatcaatc taatcatcgg ccgcaaagta ttaatatgta atagtagaag atgtttataa
     5101 tttagcgatt ccgtttcatg atttataaaa aatgaggcta ctcaaggaga tcaaataatc
     5161 tttgagtagc ctttttatag gttgtgtttg tatgcgttta cactaaaata gaaattatta
     5221 tcatgaaagt ttttggataa aaagcgttga ttattgtaaa aatactaaaa aatgagatgt
     5281 tttatttata attttctgca aatttatgat attgtttctt aatatatcat attaaaaatt
     5341 tgtttttctt aaacatagga ggcttatcta attcatggac acatcaaaac aatttagagg
     5401 tgacaaccga ttgcttttag gtatcgtttt aggggttatt accttctggc tattcgcgca
     5461 gtcacttgtt aatcttgttg tcccattaca atcaacatat agtagtgacg ttggaacgat
     5521 aaatatcgct gttagcttat ctgccttatt tgctggtttg tttatcgtag gtgctggtga
     5581 tgttgctgat aaatttggtc gcgtcaaaat tacttatgta ggattgatat taaatgttgt
     5641 aggttcatta ctcatcatta ttacaccttt gccagcattt ttaattatag gtagaataat
     5701 tcaaggtttg tctgcagcat gtattatgcc atccacactt gctattatta acgaatatta
     5761 tattggtaca agaagacaac gtgccttaag ctattggtct attggttctt ggggtggtag
     5821 tggtatttgt acgttgtttg gtggcttagt ggctacatat ataggttggc gttcaatatt
     5881 tgttgtttca attctattaa cattattagc aatgtactta atcaaacatg cacctgagac
     5941 taaagcagaa ccaatcaaag gtatgaaagc agaagctaaa aagtttgacg ttatcgggtt
     6001 agtcatttta gtagtgacaa tgttaagttt aaatgtaatc attacacaga cgtcccattt
     6061 tggtttagtt tcaccgttaa ttctaggttt aattgtagtg tttatctgtt cattagttgg
     6121 ttttgtatat tacgaaaata aaataaagca cccactcgtt gatttttcaa tttttaaaaa
     6181 tagaggatac agtggtgcaa ctatttcaaa cttcttatta aatggtgtag caggtggtgc
     6241 acttatcgtc attaacacgt attatcaaca acaattagga tttaattctt cgcaaacggg
     6301 ttatatttca ttaacgtatt taatagcagt gttgtctatg attcgtgtag gtgaaaagat
     6361 tttatctcaa catggtccga aacgcccact attactagga agtggcttta cagtgattgg
     6421 gttaatctta ttgtcgttaa catttttacc agaaatgtgg tatatcgtat ctagtatagt
     6481 tggatattta ttgtttggta ctggtttagg attatatgct acaccatcaa ctgatacagc
     6541 agttgctagt gcgccagatg ataagtcggg tgttgcttca ggcgtatata aaatggcgtc
     6601 atcattagga aatgcatttg gagtagcagt atctggtacg gtttatactg tgttagcagc
     6661 taatttaaat ttgaacttag gtggtttcac aggtatgatg tttaatgcct tgctagcaat
     6721 tgttgcattt ttagtcattt tactattagt tcctaaaaat caaacgaatt tgtaaaactg
     6781 aaatgaaagc aagttattat gtagggattt taaaggaaat tttgtgaaag taagtttatc
     6841 atacacactt aatgttgcgt attgacgttt aatgttaggt gtgttctttt atagacgata
     6901 aaagctgtgt gtatattaag cgaatgattt tcaaattgac gctaatatgc gaaagtagta
     6961 ttttaaaaat gaacaacaac gatgaagagg ggtttatagg atgaaaattg caattgctgg
     7021 atcgggtgca ttaggtagtg gctttggtgc caaactattt caagcaggat atgatgtcac
     7081 acttattgac ggatatacat ctcatgttga agcggttaag caacatggat taaatataac
     7141 gattaatgga gaggcattcg agttaaacat tccaatgtat cattttaatg atcaaccgga
     7201 cgaaagcatt tacgatgttg tctttctatt tccaaagtct atgcaattaa aagaagtgat
     7261 ggaagctatg aagccacata ttgataatga aacgattgtc gtatgtacga tgaatggttt
     7321 gaagcatgaa gaaatcattg cgcagtatgt tgctcaatca caaattgtca gaggtgttac
     7381 gacttggacg gcaggtcttg aaagccctgg acacagtcat ttacttggta gtggaccagt
     7441 tgaaataggt gaactagttg atgaaggtaa agaaaatgtt ataaaagttg ctgatttact
     7501 taacgaagcg gaattgaatg gtgtcattag taaagattta taccaatcga tttggaaaaa
     7561 gatttgtgtt aatggtacgg caaatgcatt aagcacagtg ttggagtgta atatggcatc
     7621 actgaatgaa agtagttatg cgaagtgttt gatttataaa ttaacgcaag aaatagtgca
     7681 tgtagcgacg attgataatg ttcatttaaa tgttgatgaa gtatttgaat atttagttga
     7741 tttaaatgaa aaagttggtg cgcattatcc atccatgtat caagatttaa ttgttaataa
     7801 tagaaagact gaaattgatt atattaatgg cgcagttgca acattaggta aacaacgtca
     7861 tattgaagca ccagtcaatc gctttattac tgatttaatt catactaaag aaagtcagcg
     7921 acatgcacag gattaattag tgtttttagg tgtgattgca aggaaatgac aatcacgtga
     7981 tattacggtc attattaaaa ttgaaatgta ataaataaag aacagcagta aagtactttc
     8041 aaattgaaat gatcttggtg ctgtttttct tgattggtct tcgtcataat tcagatttgt
     8101 cataggtcta cgacatacta ctagcatttg ctagacagtt tttacgacga cactttgaaa
     8161 aaaattgagg caaatcattt ggaagtctta cgtgaatttt gtacactcat caagcaagta
     8221 attatattaa aagacaaata gagaaaaggt gtttataatg agtaaaattt ttgtaactgg
     8281 tgcaacgggc cttattggca ttaaattagt tcaaagacta aaagaagagg ggcatgaggt
     8341 tgctggtttt actacatctg agaatggtca acaaaagcta gctgctgtta atgtaaaagc
     8401 atatattggt gatatattaa aagctgatac cattgatcaa gcgttagcag attttaaacc
     8461 agaaatcatt atcaaccaaa ttacggattt aaaaaatgtt gatatggcag caaatacgaa
     8521 agtgcgtatt gaaggttcta aaaacttaat tgatgcggcg aaaaagcatg atgttaagaa
     8581 agtaattgcc caaagtattg cctttatgta tgaaccgggc gaaggattag caaatgagga
     8641 aacttcactt gattttaact caactggaga tagaaaagta acggttgatg gtgtggttgg
     8701 tctagaagaa gaaacggccc gtatggatga atacgttgtt ttacgttttg gctggttata
     8761 tggtccaggt acttggtacg gaaaagatgg catgatttat aatcaattta tggatggtca
     8821 agtgacactt tcagatggcg taacatcatt tgtgcatctt gatgatgcag ttgaaacatc
     8881 tattcaagct attcattttg aaaatggtat ctataatgta gcagatgatg cacctgttaa
     8941 aggttctgaa tttgcagaat ggtataaaga acaacttggt gttgaaccaa atattgatat
     9001 tcaacctgcg caaccatttg aacgtggcgt aagcaatgag aagtttaaag cgcaaggtgg
     9061 tactctgatt tataaaactt ggaaagatgg catgaatcca attaaataat cggtttctgc
     9121 atatagcatg ttaataatga agacttgttc aaatcgctaa tcctcataca taaatatgta
     9181 tgtttcggat tgatgtgcga ttgaacaagt cttttttatg gtttcttaat tgtcgttgta
     9241 tgtaatatta acagaagtgt aattaacata ctgaataatg caataaatgc tgtagttaac
     9301 gttaatttaa tttctattgg cagccaagtc agtacaaaag accagttatt gctaccgaga
     9361 atgagatatg gtaatgcata taatatgagt gctatagcga tacataaaca taaagttagc
     9421 caacttaaaa atgtggtaag ttttgatttg cgcaggatga ttttcccgtg acgcatttgc
     9481 caagcacgat aagctgagaa tataaatgct aataaaataa gtgttgtcat caaaacggta
     9541 acaatattaa attgatcttt atattgattg agcatagacg caactgtcga atatcgcttg
     9601 tgatttacaa tttgtgtgtt aagatgctca actaatttgg gtacatattc cgaatttaga
     9661 tttgcaagca caacaattcc ataattttgt tttggattta gtaaaataaa tgatgaaaag
     9721 ttatcgagcg ttcctgaatg aaacactaaa tgttcatcat tattagtaaa ccagccggaa
     9781 gcatatgcat tggcattagg ttcaccaatt gttgatgata aatttttatg tgattgttga
     9841 actaatgatt tgtatttatc aggtggatta agttggaatt ttatccaatg ttccaaatct
     9901 tcagttgatg tcatcatata tgctgatggt gtatcccaaa ggttaaattc aggtttagag
     9961 acgacaggtg tcgaaccttg taattcgtag ccaatagcat catgttttga tttgttattg
    10021 ttttgtttga atgatgtatg tgtcatatgc aaaggcttga gccatgaatt tgtaatatat
    10081 tttgtatagg attgcttcgt aacgttttgg ataattaaac ctaataaatc atagttcata
    10141 tttgagtatt caaattcttc tccgggctta tgatgtaatt catcacccat aattgcacgg
    10201 gttacatcat ttaaacgatt atttttattt gttacggagt cttcgcttgt aatatcacta
    10261 ggtataccac ttgtttgagc caaaagttgc ttaatcgtaa tagtttcatt ttgaccatta
    10321 tagttcattt taaaatgagg cacatgtttg gatacgtcat catttaagtt taatcgacct
    10381 tcttgagcta attttaaaat tgcaagacct gtgaaagctt tcgtattaga agcaatttca
    10441 tactttgttg tgggagaagc tttgactttt ttatcaacat cttgataacc ataaccttta
    10501 tttaaaaaaa ctttgccatt ttttactatt aaaattgatg ctccaggaat atgtcccttt
    10561 tgtaaatcat gttcgataat tgtatctatt tgttgttgcg aatcattggt taaccgtgtc
    10621 ttcgtattgc tatttaatgt tatatatata gcaattgaaa tggcgactaa aatgataata
    10681 gaaatggata gaaaatacag ttttttagta gtcataaaca ttccttttta tatgttaaat
    10741 ttacttaagt gcctaatgag atttatttta ttataccagt tctattgaat aaattaatta
    10801 aagaggtata tttaccatta tttttgacac cttatatgtt aataggtgta taattacata
    10861 aaaaatatta attaactctc tatacttttt ggaaggatag gcgtatagtg atatccatca
    10921 aaaagtataa aaaggaatag gagattatgt tcaatcaatt taaaagactt attatagggc
    10981 aacctaaaaa aaacagagaa ctaaaagacg aaaagatatc gaagtttaaa gggttagcga
    11041 ttctttcgtc ggatgcatta tcttcagtgg cttacggtcc cgaacaaata ctgattacat
    11101 tatcagtagt gggtgcagtt gcaacttggt atactttacc gattgcaggt gctgttctga
    11161 tcttattagc tgctttaatt atgtcttata ggcaaattat ttatgcatat cctaaaggtg
    11221 gcggcgcgta tatggtgtcc aagaccaatt taggagaaaa atggggattg cttgcaggag
    11281 gctcattatt agtcgattac atactgactg tcgctgttag tatatcatct ggtgctgatg
    11341 catttgttgc tgcattccca agtttatatg ggcataaagt actgattgca tgtttacttg
    11401 tactttttat tttaatatta aatttacgtg gtttaactga atctgccacc gtattatcct
    11461 atccagtata tcttttcatt atagggttag tgatattaat atttataggt actttccgtg
    11521 tggcgacagg tgatattcaa ccacatatgc atgcatcagt aggaactgcg gttcctggag
    11581 taacattatt cttgctatta aaagcatttt catcaggggc ttcatcatta acaggtgttg
    11641 aagctatttc aaatgcggta accaatttta gagaaccaag tgctaataat gctgtaaaaa
    11701 cgttaattgc tatgggttca attttagcgt ttttattagt tggtatcgtt ggtttagcat
    11761 acgtttatgg aattatgcca caaacggaaa cgacagtttt atcacaatta gctatgcaaa
    11821 tatttgatga taatgctgcc ttctattttg tacaggcaac gaccgtgatg attttggtat
    11881 tagcagccaa cactggattt acagcttttc cgatgttagc ggcttcaatg tctaaagata
    11941 aatatatgcc tcctagaatg tttactgtta gaggtgatcg cttaggctac tctaactcga
    12001 ttattatttt aggtgtatta gccattattt taattattgt atttgatggt atgactgaag
    12061 atttaattcc attgtatgcg gttggtgtat tcattccatt tacactagca caattcggta
    12121 tggttattaa atggattcat gaacgtccta agaattggtt aagtaagttg tctgtgaact
    12181 tacttggtgg tatcgtgaca tttattgttt ttatgatttt actcataact aaatttagcc
    12241 aagtttggcc gatacttatt ttcttgccgt tcgtggtgat tttcttcttg aaaattaaca
    12301 aacattatcg agatatcgca gaacaattac gttctgatat tgatgttcat aatgtagaag
    12361 tagtagatcg taatttagca attgttccga taacaagcat tacaactgca gttgataagt
    12421 ctatttatta cgcacaaatg cttgcgaata atgatgttat tgcagtacat gtatcatttg
    12481 gagatgaaga tgaaaaagca ttccaagaga aatggaaacg tcatttccca gatgtaagat
    12541 tagttatttt acactcggaa tatcgcagta ttatacgacc aatttcacgt tttatcgata
    12601 agattaatcg taaagccaac gatcaaaact atatgattac agtagtgata ccagaattta
    12661 ttactaagaa acgttggcat aatttattac acaatcaaac tagtttgcgt atgaaattgt
    12721 atttaattta tcaaaagaat gttaacgtat gtacaattcc atttaaactt aaaaaataga
    12781 ttctataatc aaatcggatt gatgaaaggt tatttacttt tcatcaatcc gattttttga
    12841 tgttgactat aaaaaccccg tacatatgag atgataatga ctttgtctca tatgtacggg
    12901 ggtgttgtta tgtcttgctt aatctttgga ctgtaaagac aagacaattt cgttgtaatt
    12961 gatattttct cgaatttgag ctgcaacgat attgttgatt ttatttttgc gaattaattc
    13021 atctaaaaga tgacgttgta agtacaatgc ttccaatttg tggttatgga acatagcttg
    13081 cggtatgatt tgattgctgt tttcttgacg ttcttttcga cgttgtgcac gccactttaa
    13141 tgagcgactc atatcatagt aatgattaat gactaagcta acctcaagta catttgtact
    13201 attttgttct tcttttaagc gtaaaataat attgtgattc acaatacgca taattttttg
    13261 aacatctttg aatgaatttt caaattcttg cttcttagat tctttgttta aggctttgta
    13321 gtgcttcatc gctttaagtc taagccataa catcttgaag aagtttgcaa atttttcaac
    13381 aaatgatgca gtacggtgtg tctctgttaa ttcgacaata ttgcggtaat tattaatatc
    13441 ctggtatgtt gccttacctt tatcgattaa acgctcaagt gtagatgttt cgattacttt
    13501 tgcaatatct tctaaacgtt tgagttcttt tgtattttga ttatcaggtt ctgaatttaa
    13561 taaaaataac aattctccat agtattggtt taatactggg cgataatttg tatcgttttt
    13621 gtctttttta ctttcgtttt taaaatgctg gatcactttt tgaacaatga aaattttggc
    13681 agattgataa gtcatacctt taaaagtagt atcttcggca gatggtgtaa ttaaaggtaa
    13741 aacaatttgc gctaaaatta aactaattaa taccatgaaa gatgcaataa acaatagatc
    13801 attacggtat tcaaaagctt gcccttgtgt aataataaac ggcaatgtaa gtgccattga
    13861 aagtgaaatt gtaccgtgaa taccacacat cgtcataata aatgcgtaac gcacacgaga
    13921 aggtggtgtt tcatgtgaat catgttcctc gtctagataa gattgtatat ttttcgggaa
    13981 ataaaaatct ttgtaccaga aataaaccca aacaaatcga catgcataaa ttgcaatagc
    14041 gattaatata gtgatcacta ttaaaaatga aaagttgtca ggctctgttt ggaaaatatc
    14101 tatgactaca gtaggaatca taaaacctaa tacaacaaat acaaaaccat ttaaggcata
    14161 actaaatgtg ttccaaattt gatggtaatt catttgtagt tccgtttgtg ctctaattaa
    14221 acggtcgcgt tctaaaccat ggattagacc tgcgattaca actgcaatga tacctgaagc
    14281 atgaacttct tctgctaaaa agtatacgac aaaaggagtt aataattgaa taaaagttaa
    14341 ggtattgtta tcttttaaac ctttattagc ggttaagtct atacgtattc taacgacaac
    14401 gaatccaata attgcaccaa taagtacacc tagtattgtt gaaatgataa attgttcaat
    14461 agcttggaat aatgaaaagg taccagttac taatgctgta acagcgattt tgaaagaaat
    14521 gatacctgca gcatcattga gtaaagattc accttctaaa atcgtcatag aacctttagg
    14581 taataatttt ccgcgtgtaa tagcagatac tgctactgca tcagtaggac ataaaattgc
    14641 tgctattgca aaagcggctg gcattggtaa ggcaggccaa atccaatgta taaaatagcc
    14701 aacaccgact acagttgcaa acactagtgc cattgacatt aatagtatag gtttgcgata
    14761 ttctaataat tttgttcgag agacgtgggt accttccaca aaaagtagtg gcgcgataac
    14821 ggcaaacata aatacttcag aattgaattg gaaatcaact tgtattggaa taatgaaaat
    14881 aacgacacct aatgcaattt gaataaaggc agtaggaatt tgtggaaatc gattattgat
    14941 aaccgaacta ataatcacag caaaaataaa aattaaaaat gcttctaata gtgccataca
    15001 atactcctca aaattttaat agttaatatt ttatcacttt taaggcataa tgacatagat
    15061 atattgataa aatgaagtta ttttcaaaaa aactctagta tcggttgaac tgatactaga
    15121 gcgagatgtt taaattattg attgtcatat ctgaaatgac cgctgtcatt ttgtcgttgt
    15181 tcatatgcga gcttttcagc attcgttttg tattttttat aaaagaaaaa taaaaatata
    15241 aaccagaatg gcgaaatata aatagctgct cttgtttcgt cactaaagaa taataaaatg
    15301 aatacaaaga agaagaaagc taaaataatg taagcaatag gcttaccacc aatcaactta
    15361 aatttactgt ttttatgtgc ctcaggatgc tttttcaaat acattagata agcgacaata
    15421 atcattgccc aaacaactaa aaacaacaca gttgataacg ttgtaacgta tatgaatagt
    15481 tgaattgcat taggaaaaat gtagtttaac aaggctgcta taagtaatgc aattgatgaa
    15541 actaacattg ataaataagg cacgccatga ctattcgttt tatttaaaac tttaggacct
    15601 aacccttgtt gtgacagtcc gaataagata cgactattcg aatagattcc actatttgta
    15661 gcagaggccg cggcagttag cacgacaaag ttaacgacac ctgctgcaaa tggcacgccg
    15721 attaatgtaa agagtttaac gaaagggcta ctatttggat cgatatcatt ccaaggtatg
    15781 actgacataa ttactaatag accaccgata tagaataata aaatacggat aggtacatta
    15841 ttaattgctt tcggtaaagt cttttcagga tctttcgttt cgccagcagt tacacctata
    15901 agttcaatac caatgaatga atatacagca atttggaatg acattaagaa accaaatgtc
    15961 cctccaggga acatgccgcc gtgactgatt aagtttgtga atgatgcatg tccataatgt
    16021 gttttaaatg agaaaaagat taatacaaga ccaacgataa ccatcgcaat aattgtaaca
    16081 actttaataa tagagaacca aaactccagt tcaccaaata atctggcacc taataagttg
    16141 aagctaatca agattaaaac aataaatagc acggtaatcc aatttgggac ttgtggatac
    16201 caaaatgcaa agtattgtcc catcgcagtt aggtctgaca tacttgatac aatccaacat
    16261 agccagtaag tccagccaat gacaaagcca ccaaaagggc ctaaatattc atttgcaata
    16321 tcaacaaatg aattaaatct tgtattgctc aacaacaatt cgcctaatgc gcgcataaaa
    16381 gcgaatagta caaccccaat aatcatgtat gtgaataaca gtgatggacc agttaaggat
    16441 attgtttgac ctgatcctag gaataaccca gttccaattg cgcccccaat tgctatcaat
    16501 tggatgtgtc tgttattcaa ctctctatgc aattttcttg ccataatttt cctcctcagg
    16561 ataaagcaaa taaaaaagtt gtgtgtaagc gttttcttaa ttataaatac taaagaaatc
    16621 catttaataa agcaattatt tttttaaata taagaaaaaa catttgatag ttaagggtat
    16681 gataactaaa acttatcgat ttttcgaaaa gatataagtt tttatgcaaa aatataaaat
    16741 aatcatctaa atgaagcagt taactatata tattagataa gctatggcat aataaataac
    16801 attttaattt gagtaaagaa ggtatgggta tgaagatagg gacaatcgcc gatttacata
    16861 ttgatcgtca taataaaaaa acatccgaag attacttaga ggcattggtt gaaatagtta
    16921 agtataaaaa tttggatata ttgttgattg cgggggatat ctcaaatcat tatcaattga
    16981 cgcaccaatt tataacgcaa ttgacaaaac aattagatat acctgtgaaa tttgttcctg
    17041 gaaaccatga tttatgggaa gttgaaagta tgactacgca aga
//
LOCUS       DAMZPP010000056        16771 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMZPP010000056 DAMZPP010000000
VERSION     DAMZPP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16771
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.056"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..789
                     /gene="rsmB"
                     /locus_tag="POH73_001365"
     CDS             <1..789
                     /gene="rsmB"
                     /locus_tag="POH73_001365"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDI6887326.1"
                     /translation="TTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQ
                     PIIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQ
                     VDASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRH
                     KPEIKYTQSKQHIESLVDLQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLK
                     NNKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            792..1886
                     /gene="rlmN"
                     /locus_tag="POH73_001366"
     CDS             792..1886
                     /gene="rlmN"
                     /locus_tag="POH73_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDI6887327.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            1893..2636
                     /locus_tag="POH73_001367"
     CDS             1893..2636
                     /locus_tag="POH73_001367"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HDI6887328.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            2633..4627
                     /gene="pknB"
                     /locus_tag="POH73_001368"
     CDS             2633..4627
                     /gene="pknB"
                     /locus_tag="POH73_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HDI6887329.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            4855..5730
                     /gene="rsgA"
                     /locus_tag="POH73_001369"
     CDS             4855..5730
                     /gene="rsgA"
                     /locus_tag="POH73_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDI6887330.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFDVQNINEGYIHQVYERKNELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARILVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWSAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            5731..6375
                     /locus_tag="POH73_001370"
     CDS             5731..6375
                     /locus_tag="POH73_001370"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDI6887331.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            6382..7023
                     /locus_tag="POH73_001371"
     CDS             6382..7023
                     /locus_tag="POH73_001371"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDI6887332.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(7404..7592)
                     /locus_tag="POH73_001372"
     CDS             complement(7404..7592)
                     /locus_tag="POH73_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDI6887333.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
     gene            8035..8409
                     /locus_tag="POH73_001373"
     CDS             8035..8409
                     /locus_tag="POH73_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDI6887334.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            8424..10070
                     /gene="fakA"
                     /locus_tag="POH73_001374"
     CDS             8424..10070
                     /gene="fakA"
                     /locus_tag="POH73_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HDI6887335.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKLDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLADDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            10260..12320
                     /gene="recG"
                     /locus_tag="POH73_001375"
     CDS             10260..12320
                     /gene="recG"
                     /locus_tag="POH73_001375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDI6887336.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFLNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDLDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLI
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENRTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEIDVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            12523..13095
                     /gene="fapR"
                     /locus_tag="POH73_001376"
     CDS             12523..13095
                     /gene="fapR"
                     /locus_tag="POH73_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDI6887337.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILGITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            13100..14086
                     /gene="plsX"
                     /locus_tag="POH73_001377"
     CDS             13100..14086
                     /gene="plsX"
                     /locus_tag="POH73_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDI6887338.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNQDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKIAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            14079..15005
                     /gene="fabD"
                     /locus_tag="POH73_001378"
     CDS             14079..15005
                     /gene="fabD"
                     /locus_tag="POH73_001378"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDI6887339.1"
                     /translation="MSKTAITFPGQGAQKVGMAQDLYNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPSGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            14998..15732
                     /gene="fabG"
                     /locus_tag="POH73_001379"
     CDS             14998..15732
                     /gene="fabG"
                     /locus_tag="POH73_001379"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDI6887340.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            16038..16271
                     /locus_tag="POH73_001380"
     CDS             16038..16271
                     /locus_tag="POH73_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDI6887341.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            16387..>16771
                     /locus_tag="POH73_001381"
     CDS             16387..>16771
                     /locus_tag="POH73_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDI6887342.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPS"
BASE COUNT         6150 a         2252 c         3285 g         5084 t
ORIGIN      
        1 acgacatcaa caactgtgcg tgccaacctg acgcgagcat cattagatga tattattgaa
       61 aagttgcaag acgaaggtta tgacgttgaa aaagatcatg acttacctta ttgtctccat
      121 ataggaggac aaccaattat tcattctcgt tcatttaaag atggattcgt ttcaattcaa
      181 gataaaagct caatgtttgt tgcacacatt atgaatgtag accgacatga tcacgtatta
      241 gatgcatgta gtgcacctgg cggtaaggct tgtcatattg ctgaagtttt aatgccagaa
      301 gggcaagttg acgcttcaga tatacatgat cacaaaatag acttaattaa ttttaatata
      361 aaaaaattac gattaacaaa tattaaagct tttcaacatg atgcgacaaa accttatgat
      421 aaaacatacg ataagatact tgttgatgca ccatgtagcg gattaggtgt tatgagacac
      481 aagccggaga ttaaatatac tcaaagcaaa caacatattg agtcactagt tgatttacag
      541 cttgaaatat tggaaaatgt aaaaaacaat gtaaaaatag gtggcgaaat catctattcc
      601 acatgtacaa tagagcaact agaaaatgaa aacgtgattt atacgttttt gaaaaataat
      661 aaaaacttcg aatttgaacc gtttcaacat ccgataactg gagagttggt caaaacgtta
      721 caaatcatgc cgcaagactt taattcagat ggattcttta tcactaagat aaaaagaaag
      781 gacaattagg aatgataact gctgaaaaga aaaagaagaa taaatttctt ccaaattttg
      841 acaagcaatc aatatattca ttgcgatttg acgaaatgca aaactggctc gttgaacaag
      901 gtcaacaaaa atttcgagcg aaacagattt ttgaatggtt atatcaaaaa agagtagatt
      961 cgattgatga aatgacgaac ttatcgaaag acttacgaca gcttttaaaa gataacttta
     1021 ctgttacaac tttaacaact gtagtaaaac aagaaagtaa agacggtaca attaaattct
     1081 tatttgaatt acaagatggc tatacaattg aaactgtttt aatgagacat gattatggaa
     1141 attcagtatg tgtaacgaca caagtaggtt gtcgtatcgg atgtacgttt tgtgcttcta
     1201 cacttggcgg cttaaaaaga aaccttgaag ctggcgaaat tgtttcacaa gttttaacag
     1261 ttcaaaaagc ccttgatgct acagaagagc gcgtatctca aattgtcata atgggtatcg
     1321 gtgaaccatt tgaaaattat gatgaaatga tggacttttt aagaatcgtc aatgatgata
     1381 acagtttaaa tattggtgca cgtcacatta cagtatcaac atcaggtatc attcctagaa
     1441 tatatgactt tgcggacgaa gatattcaaa ttaattttgc tgtaagctta cacgccgcaa
     1501 aagatgaagt gcgatcacgc ttgatgccaa ttaaccgtgc atataatgtt gagaagttaa
     1561 tcgaagcaat tcaatattat caagaaaaaa caaatcgtcg tgttactttt gaatatggtc
     1621 tgtttggtgg tgtgaatgac caactagaac atgcaagaga attagcacat ttaataaaag
     1681 gcttaaactg ccatgttaac ttaattcctg tcaaccatgt tccagaaaga aattatgtaa
     1741 aaacggctaa aaatgatatc tttaaatttg aaaaagaatt aaagagacta ggaattaatg
     1801 ccacaatacg tcgtgaacaa ggttcagata ttgacgcagc ttgtggtcaa ttaagagcaa
     1861 aggaacgaca agtagaaacg aggtaaagac aaatgctaga ggcacaattt tttactgata
     1921 ctggacaaca tagagataag aatgaagatg cgggtggtat tttttataat caaactaatc
     1981 aacaactttt agttctgtgt gatggtatgg gcggccataa agcaggagaa gttgcaagta
     2041 aatttgttac agatgagttg aaatcccgtt ttgaagcgga aaatcttata gaacaacatc
     2101 aagctgaaaa ttggttgcgt aataatataa aagatataaa ttttcagtta tatcactatg
     2161 cacaagaaaa tgcagaatat aaaggtatgg gtacaacatg tgtttgtgca cttgtttttg
     2221 aaaaatcagt tgtgatagca aatgtcggtg attctagagc ctatgttatt aatagtcgac
     2281 aaattgaaca aattactagt gatcactcgt ttgttaatca tcttgtttta acgggtcaaa
     2341 ttacgccgga agaagcattt acacatccac aacgtaatat tattacgaag gtgatgggca
     2401 cagataaacg tgtgagtcca gatttgttta ttaagcgatt aaatttttat gattatttat
     2461 tattaaattc agatggattg actgattatg ttaaagacaa tgaaattaag cgtttgttag
     2521 taaaagaagg tacaatagaa gatcatggtg atcaattaat gcaattggca ttagataacc
     2581 attcgaaaga taacgttact ttcatactcg cggctattga aggtgataaa gtatgatagg
     2641 taaaataata aatgaacgat ataaaattgt agataagctt ggcggcggtg gcatgagtac
     2701 cgtttatctt gcagaagata cgatacttaa cattaaagtt gcaattaagg cgatttttat
     2761 accacctaga gaaaaagaag aaacattaaa acgttttgaa cgagaagtac ataactcatc
     2821 acagctatca catcaaaata tagtaagtat gatcgatgtt gatgaagaag atgactgtta
     2881 ctacttagta atggaatata ttgaaggtcc gactttgtct gagtatattg aaagtcatgg
     2941 gccattaagt gttgacacgg cgattaattt tacgaatcaa atattggatg gcattaaaca
     3001 tgcgcatgat atgcgtattg tacatagaga tattaagcca caaaatatat taattgacag
     3061 caataaaacg ttgaaaatat ttgattttgg aattgctaaa gctttaagtg agacgtcttt
     3121 aactcagact aatcatgtgt taggtactgt gcagtacttt tcgccagaac aagcaaaagg
     3181 tgaggcaacg gatgaatgta cagatattta ttctataggt attgtgttat atgaaatgct
     3241 tgttggtgaa ccaccattta atggagaaac tgcagttagc attgcgatta aacatattca
     3301 ggattctgtg ccaaatgtga caaccgatgt acgtaaggat attccgcaat ctttaagtaa
     3361 tgtcatttta cgcgctacag aaaaagacaa agcgaatcgt tacaaaacaa ttcaagaaat
     3421 gaaagatgat ttgagtagtg ttttacatga aaatcgagcg aatgaagatg tctatgaact
     3481 cgataaaatg aaaacgatag cggtaccatt gaaaaaagaa gatctagcaa agcatattag
     3541 tgaacataag tcgaatcaac ctaaacgtga aacgacgcaa gtacctattg taaatgggcc
     3601 tgctcatcat cagcaattcc aaaagccaga aggtaccgtg tacgaaccaa aacctaaaaa
     3661 gaaatcaaca cgaaagattg tgctcttatc actaatcttt tcgttgttaa tgattgcact
     3721 tgtttctttt gtggcaatgg caatgtttgg taataaatac gaagagacac ctgatgtaat
     3781 cgggaaatct gtaaaagaag cagagcaaat attcaataaa aacaatctga aattaggtaa
     3841 aatttctaga agttatagtg ataaatatcc tgaaaatgaa attattaaga caactcctaa
     3901 tacaggtgaa cgtgttgaac gtggtgacag tgttgatgtt gttatatcaa aaggccctga
     3961 aaaggttaaa atgccaaatg ttattggttt acctaaggag gaagccttgc agaaattaaa
     4021 atcgttaggt cttaaagatg ttacgattga aaaagtatat aataatcaag caccaaaagg
     4081 atacattgca aatcaaagtg taaccgcaaa tactgaaatc gctattcatg attctaatat
     4141 taaactatat gaatctttag gcattaaaca agtttatgta gaagactttg aacataaatc
     4201 ctttagcaaa gctaaaaaag ccttagaaga aaaaggattt aaagttgaaa gtaaggaaga
     4261 gtatagtgac gatattgatg agggtgatgt gatttctcaa tctcctaaag ggaaatcagt
     4321 agatgagggg tcaacgattt catttgttgt ttctaaaggt aaaaaaagcg actcatcaga
     4381 tgtcaaaacg acaactgaat cggtagatgt accatacact ggtaaaaatg ataagtcaca
     4441 aaaagttaaa gtttatatta aagataaaga taatgacggt tcaacagaaa aaggtagttt
     4501 cgacattact agtgatcaac gtatagacat tcctttaaga attgaaaaag gaaaaacagc
     4561 aagttatatt gttaaagtag acggtaaaac tgtagctgaa aaagaagtca gctatgatga
     4621 tgtttaaata taattgaagt aaatgtaccg aggtttctat ttggaagtct cggtattttt
     4681 atgttgagat tgcggtagtt ttaaaatgct tcatgtcatc atatacaggt gagcattgtc
     4741 tatgaattct gtatcactct cacaatattt atatcatttg aaatgatcat ttatatatta
     4801 attttccagt ctttgatata ataaatgcag taaaattaat cgagaggtgc cattttgaag
     4861 acaggtcgaa tagtgaaatc aattagtggg gtatatcaag tagacgttaa tggcgaacgt
     4921 ttcaatacaa aaccacgagg attatttaga aagaaaaaat tttcaccggt agttggagat
     4981 atagtggaat ttgatgtaca aaacattaac gaaggctata ttcatcaagt gtatgagcgg
     5041 aaaaatgagt tgaaaagacc acctgtaagt aatatagata cactagtaat tgtaatgagt
     5101 gctgtcgaac caaatttttc aacgcaatta ttagatcgat ttttagttat tgcacattcg
     5161 tatcagttaa atgcgagaat tttggtgaca aaaaaagata aaacaccaat tgaaaagcag
     5221 ttcgaaatta atgagttgtt gaaaatatat gaaaatattg gctatgagac tgaatttatt
     5281 ggaaatgatg atgatcgaaa aaaaattgta gaagcttggt cagctggact tatagtactt
     5341 agtggtcaat caggtgtcgg taagtccact ttcttaaatc attatcgtcc agaacttaat
     5401 cttgagacaa atgatatatc aaaatcatta aatcgaggaa agcatactac aagacatgtc
     5461 gaactattcg aacgtcaaaa cggttatatt gcagacacac ctggattcag tgctttagat
     5521 tttgatcata tagataaaga tgaaataaaa gattattttc ttgaattaaa tcgatatggt
     5581 gaaacatgta agtttaggaa ttgtaatcat atcaaagaac ctaattgtaa tgttaagcat
     5641 caattagaga tagggaatat tgcgcaattt agatacgacc attatttaca actatttaat
     5701 gaaatttcaa atagaaaggt tagatattaa atgacaaaac tatatccatc attattatct
     5761 gttgattttt tggatttaca acatgaatta aaacgacttg aagaagcagg tgtcgacgga
     5821 gttcattttg atgttatgga tggtcaattt gtgcctaata tatctattgg tttaccaata
     5881 ttagatgcag taagaaaagg cacaacatta cctatagacg tacatttgat gattgaaaat
     5941 ccagaaaagt atattgcatc atttgcagaa catggtgccg atatgatttc aattcatgtc
     6001 gaatcaacgc ctcatattca tcgtgctatt caaatgatta aacatttaga taaaaaagct
     6061 ggtgtagtaa ttaatcctgg tacaccaata tcacaaattg aacctatttt agatattgtt
     6121 gattatgtac tagtgatgac agttaaccca gggtttggtg gtcaatcatt tattgatcaa
     6181 tgcgtagaaa aaatagcggg tcttaatgct attaaaatgg aacgtcaatt aaactttgat
     6241 attgaagttg atggaggcgt aaataccgat acagcgaaag tatgtgttga aaatggtgct
     6301 acaatgctag taacaggttc atttttcttt aaacaagagg attataaaaa agtcacacaa
     6361 gcattgaaag gttgagtgta tatgcatata aatttattat gttctgatcg acacttgccg
     6421 caagatattt gggccaaaag taatgaaggt aaatggggcg gcgttgatag aggtgctttg
     6481 attttattga agcatcaaat tatccctttt ttctcagtgg gagactttga ttcagtcagt
     6541 aaagaagaac gccaacttct aacagaacag ttacaaatca aaccagttca agctgaaaaa
     6601 gctgatacgg atttagcttt agcggttgat aaagctgttg cacttggatt tgatagtatt
     6661 acaatttatg gtgcaacagg cggacgatta gatcactttt ttggggcaat tcagttatta
     6721 ttgaaaaaag catattataa acatgatgtt catatagaag ttatcgatca acaaaataaa
     6781 attgaattat tgcctaaagg tcaacataca gttgaaaaag ataagagtta tccgtacatt
     6841 tcatttatac cgatgactga tgatgtagaa ctttctctag caggttttaa atataattta
     6901 gctagacaaa tgcttaatat aggttctact ttaactattt caaatgaaat tgagtctttg
     6961 caagcgaaag taactgtaca tgatgggttg attttgcaaa ttagaagtac agatttaaat
     7021 taagtatatt caaatatgcg aagattttta ataaagtcca tgagttattt aataaagtga
     7081 cgtttatagt atagaattta tagcataata atagatgaat cacgctgtaa ttaatgaaga
     7141 ttatggcgtg atttattatt gaaaggaaaa ttgtaggtcc tagtacattg atttatgtgc
     7201 caaaatcgat attgaaccca taagtccaat ttgacagaga accattatta aacgttttaa
     7261 ccaaattata ttgatagttc gaatttcact atgaaccatt atgtattgtt ttgtaacaaa
     7321 aaagaactaa gaggttgagc tcttagttct taagtaaatg cgtcctccac ataaggtcac
     7381 attttttatg tgcaatatat ttattaaact ctagttactt taccagattt taaagcacgt
     7441 gcagaaaccc aaactttttt aggtttaccg tcaactagga ttctaacttt ttgaaggtta
     7501 gcgttccatc tacgtttagt agagtttaaa gcgtgtgaac gtctgttacc agtcgaagct
     7561 ttacgacctg ttacgaaaca ttgtttaccc atatgagtac ctcctttaat aaatataaat
     7621 acacataact acttatatac ttaattaaga tagcatagtt tcatttgaaa aacaatgaat
     7681 aattttcaca taaaagtcaa aaatactgag ttttgtgata taattgtaga ctgtgaagtt
     7741 atgtagtatg atattttaga gaaataagtg aatgaaacat ttaaactttt tatttaatga
     7801 tactacatct attaaaagaa aatgcctata cataatcgtc atgagtgtaa attcagttta
     7861 ctcaaaatat aataggtgat ttaagcgtaa gtcattctga taagttgtac cattgatatt
     7921 ttataaaaat aatcaatgaa acttaaagcg tttatgacta cactaagtgg taaaatttta
     7981 aaagtgatta ggggaaacga ataataaact ttgaaattta ggagggacaa agatatgaca
     8041 ttagagattt caaatgatta cggtaaaatt gatatttcaa acgaagtgat tgcttcggtt
     8101 gtaggtggaa aggccgttga atgttatggt attgtaggta tggcatctag acaacaagtt
     8161 agagatggta ttgcggaaat actaggacat gaaaattatg ctaaaggcat caaagtaact
     8221 gaaaataatg gcgtagtgga tatagatatg tacattattg taagttatgg tgtgaaaata
     8281 tctgaagttg ccaataatgt acaatcaaca gtgaaatata ctttggaaaa atcacttaat
     8341 gtatcagtaa attcaatcaa tatatatgta caaggtgtac gtgtgaataa tacaggcaag
     8401 aaagcttagg aggacaactt gaaatgatta gcaaaattaa tggtaaatta tttgccgata
     8461 tgattataca aggggcacaa aatttatcta acaatgcaga tttggtagat tctttgaatg
     8521 tgtatccagt gccagatggt gatacaggaa caaatatgaa tcttactatg acttcaggtc
     8581 gcgaagaagt agagaataat ttgtcgaaaa atatcggcga attaggtaaa acattctcga
     8641 aaggtttact aatgggtgca agaggtaact ctggtgtcat cttgtcacaa ttattcagag
     8701 gattttgtaa aaatattgaa agtgaatctg aaattaattc aaaattgtta gctgaaagtt
     8761 ttcaagctgg tgttgaaacg gcatataaag ctgttatgaa accagttgaa ggtacaatac
     8821 ttacagttgc aaaagatgct gcgcaagctg caatagaaaa agcaaataat actgaagatt
     8881 gtatagaatt aatggagtac attattgtaa aagccaatga atcacttgaa aacacaccaa
     8941 acttattagc tgtacttaaa gaagttggtg ttgttgatag tggcggtaaa ggtttgttat
     9001 gcgtttacga aggattctta aaagcgctta aaggtgaaaa agttgaagcc aaagttgcaa
     9061 agctagataa agatgaattt gtacatgatg aacatgattt ccatggtgta attaatactg
     9121 aagatattat ttatggctat tgtactgaaa tgatggttcg ttttggaaag aataaaaaag
     9181 cctttgatga acaagaattc aggcaagata tgagtcaatt tggtgattct ttattagtca
     9241 ttaatgatga agaaattgtg aaagttcacg tgcataccga atacccaggt aaagtgttta
     9301 attatggtca acaatatggt gaattaatta aacttaaggt tgaaaatatg agagaacagc
     9361 atcgtgaagt gattcgaaaa gaacagcaca cagctaaacc gaaaatggaa acggttgaaa
     9421 cagcaattat tactatttct atgggtgaag gtatttcaga gatatttaaa tcaatgggtg
     9481 ccacacatat cattagtggt ggacaaacga tgaatccttc tacagaagat atcgttaaag
     9541 tcattgaaca atcaaaatgt aaacgtgcaa ttattttacc gaataataaa aatatcttaa
     9601 tggcaagtga acaagcagcg agtattgttg atgcagaagc agttgttatt ccaacgaaat
     9661 ctattcctca aggtataagc gcactattcc aatatgatgt ggacgcaaca cttgaagaaa
     9721 ataaagcgca aatggctgat tcagtaaata acgttaaatc tggttcatta acgtacgctg
     9781 ttcgtgatac gaaaattgat ggcgttgaga ttaaaaaaga cgcgtttatg ggcttgattg
     9841 aagataagat tgtaagcagc caaagtgatc aattaacaac ggttactgag ttgttaaatg
     9901 agatgttagc agatgatagt gaaatattga ctgtgattat tggtcaagat gcagagcaag
     9961 cagttacaga taacatgata aactggattg aagagcaata tccagatgta gaagtggaag
    10021 ttcatgaagg tggacaacca atttatcaat atttcttttc agtagaataa aaatttaaaa
    10081 taaaaaacta ccaatgataa atcatcagtt ggtagttttt tattttgcta ttttagtgat
    10141 attgcgggtt aaaagtatcg ttctcgagtt gctaacaatg tcatgttcaa cttagtcatg
    10201 ataaaataaa taacatacta aatgatacgt aaaatcaaat aaaacatagg tgatttattt
    10261 tggctaaagt aaacttaata gaaagtccat attctctttt acaattaaaa ggtataggtc
    10321 ctaagaaaat agaagtattg caacaactaa atattcatac agtggaagat cttgttcttt
    10381 atttgccaac tagatatgaa gataatacag tgattgattt gaatcaagca gaagatcaat
    10441 ctaacgttac gatagaagga caagtatata cagctccagt agttgcattt tttggaagaa
    10501 ataaatcaaa attaactgtt catttaatgg taaataatat tgctgtcaaa tgtatttttt
    10561 tcaatcaacc gtatttaaaa aagaaaatcg aattaaatca aactataact gttaaaggta
    10621 agtggaatag ggttaaacag gaaattactg gtaatagggt tttcttgaat tcacaaggta
    10681 cacaaactca agaaaacgca gatgttcaat tagaaccagt ctatcgtatt aaggaaggta
    10741 ttaaacaaaa gcaaatacga gaccaaatta gacaagcgtt aaatgatgtg acaattcatg
    10801 aatggttaac tgatgaacta agagaaaaat ataaattaga gaccttggac tttactttga
    10861 acacgttaca tcatcctaaa agtaaagagg atttattacg tgctcgtaga acctatgcat
    10921 ttactgaact gtttttattc gaattacgta tgcaatggct aaatagatta gaaaagtcat
    10981 ctgacgaagc aattgaaatt gattatgact tagaccaagt taaatcattt attgatcgtt
    11041 tgccttttga actaactgaa gcacagaaat ccagtgttaa tgaaattttt agagatttaa
    11101 aagcaccaat acgtatgcat cgattacttc aaggtgatgt aggttcagga aaaacagtag
    11161 ttgctgcaat ttgtatgtat gcgttaaaaa cggctggtta tcaatcagca ttgatggtac
    11221 caactgaaat tttagcagag caacatgctg aaagtttaat tgctttattt ggagattcta
    11281 tgaatgttgc gttgttaact gggtcagtaa aaggtaagaa acgaaaaata cttttagaac
    11341 aacttgaaaa tcgtacgatt gattgtttaa ttggaaccca tgcattgatt caagatgatg
    11401 tgattttcca taatgttggt ttagtaatta cagatgaaca acatcgattt ggtgtgaatc
    11461 aacgccaact tttaagagaa aaaggtgcaa tgacgaatgt gttatttatg acagcaaccc
    11521 cgataccaag aacactagca atatcagttt ttggtgagat ggatgtgtct tcaattaaac
    11581 aattaccaaa aggtcgtaaa cctatcatta ctacttgggc aaagcatgag caatacgata
    11641 aagttttgat gcaaatgacc tcagagttga aaaaaggtcg tcaagcatat gttatttgcc
    11701 cgcttataga aagttctgag catctcgaag atgttcaaaa tgttgtcgca ttgtacgagt
    11761 ctttacaaca gtattatggt gtttcccgtg tagggttatt gcatggtaag ttgtctgccg
    11821 atgaaaaaga tgaggtcatg caaaagttta gcaatcatga gatagatgtt ttagtttcta
    11881 ctactgttgt tgaagtaggt gttaatgtac cgaatgcaac ttttatgatg atttatgatg
    11941 cggatcgctt tggattatca actttacatc agttacgcgg tcgtgtgggt agaagtgacc
    12001 agcaaagtta ctgtgtttta attgcatccc ctaaaacaga aacaggaatt gaaagaatga
    12061 caattatgac acaaacaacg gatggatttg aattgagtga acgagactta gaaatgcgtg
    12121 gccctggaga tttctttggt gttaaacaaa gtggattgcc agatttctta gttgccaatt
    12181 tagttgaaga ttatcgtatg ttagaagttg ctcgtgatga agcagctgaa cttattcaat
    12241 ctggcgtatt ctttgaaaat acgtatcaac atttacgtca ttttgttgaa gaaaatttat
    12301 tacatcgcag ttttgactaa ttaccatgct gatttgtcaa tttaagtgca acacttcgtt
    12361 aattgagtga tatgacactt gaactattta aatgtaaagt ggtattttaa aaatttataa
    12421 attttcgact aaataatagc taaataatac agttatttgt tgagtcggtt aaatagaaag
    12481 tgttatgata tgtgaggaat gtttaagact aggtactaaa aaatgagggg tgagacgttg
    12541 aaactaaaga aagataaacg tagagaagca atcagacaac aaattgatag caatcccttc
    12601 atcacagacc atgaactaag cgacttattt caagtgagta tacaaacaat tcgtttagat
    12661 cgcacttatt taaacatacc agaattaagg aagcgtatta aattagttgc tgaaaaaaat
    12721 tatgaccaaa taagttctat tgaagaacaa gaatttattg gtgatttgat tcaagtcaat
    12781 ccaaatgtta aagcgcaatc aattttaggt attacatcgg attctgtttt tcataaaact
    12841 ggaattgcgc gtggtcatgt gctgtttgct caggcaaatt cattatgtgt tgcgctaatt
    12901 aagcaaccaa cagttttaac tcatgagagt agcattcaat ttattgaaaa agtaaaatta
    12961 aatgatacgg taagagcaga agcacgagtt gtaaatcaaa ctgcaaaaca ttattacgtc
    13021 gaagtaaagt catatgttaa acatacatta gttttcaaag gaaattttaa aatgttttat
    13081 gataagcgag gataaaatta tggttaaatt agcaattgat atgatgggtg gcgacaatgc
    13141 gcctgatatc gtattagaag ccgtacaaaa ggctgttgaa gactttaaag atctagaaat
    13201 tatacttttc ggtgacgaaa aaaagtataa tctgaaccat gaacgaatcg aatttagaca
    13261 ttgttctgaa aagattgaaa tggaagatga gcctgttaga gcgattaaac gtaaaaaaga
    13321 tagctcaatg gtaaaaatgg ctgaagctgt gaaatctggt gaggcagatg gatgtgtgtc
    13381 agcaggtaat actggtgctt taatgtcagc tggtttattc attgttggac gtattaaagg
    13441 tgtagctaga ccggctttag tagtaacatt gccaacgatt gatggaaaag gttttgtctt
    13501 tttagacgtt ggtgcaaatg ctgatgctaa gcctgaacac ttattacagt atgcgcaact
    13561 aggggatatt tatgctcaaa aaattagagg tattgataat ccgaaaatct cattattaaa
    13621 tataggtacc gagccagcta aaggtaatag tttaacgaaa aaatcatatg agttattaaa
    13681 tcaagatcat tcattgaatt ttgttgggaa tattgaagcg aagacattaa tggatggcga
    13741 tacagatgtt gtagttaccg atggctatac tgggaacatg gtccttaaaa atttagaagg
    13801 tactgcaaaa tcaatcggta aaatgttaaa agatacgatt atgagtagta ctaaaaataa
    13861 aatagcaggt gcaatattga agaaagattt agcagaattc gctaaaaaga tggattactc
    13921 agaatacggt ggttccgtat tattaggatt ggaaggtacc gtagttaaag cacacggtag
    13981 ttcaaatgct aaagcttttt attctgcaat tagacaagca aaaatagcag gagagcaaaa
    14041 tattgtacaa acaatgaaag agactgtagg tgaatcaaat gagtaaaaca gcaattactt
    14101 ttccgggaca aggtgcccaa aaagttggta tggcacaaga tttatataac aacaacgatc
    14161 aagcaactga aattttaact tcagcagcaa acacattaga ctttgatatt ttagagacaa
    14221 tgtttactga cgaagaaggt aaattgggtg aaactgaaaa cacgcaacca gctttattga
    14281 cgcatagttc ggcattatta gcagcactaa aaaatttgaa tcccgatttt actatggggc
    14341 atagtttagg tgaatattca agtttagttg cagctgacgt attatcattt gaagatgcag
    14401 ttaaaattgt tagaaaacgt ggtcaattaa tggcgcaagc atttccttct ggtgtaggaa
    14461 gcatggctgc agtattgggc ttagattttg ataaagttga tgaaatttgt aagtcattat
    14521 catctgatga caaaataatt gaaccagcaa acattaattg cccaggtcaa attgttgttt
    14581 caggtcacaa agctttaatt gatgagctag tagaaaaagg taaatcatta ggtgcaaagc
    14641 gtgtcatgcc tttagcagta tctggcccat tccattcatc gctaatgaaa gtgattgaag
    14701 aagatttttc aagttatatt aatcaatttg aatggcgtga tgctaagttt cctgtagttc
    14761 aaaatgtaaa tgcgcaaggt gaaactgaca aagaagtaat taaatctaat atggtcaaac
    14821 aattatattc accagtacaa ttcattaact caacagaatg gctaatagac caaggtgttg
    14881 atcattttat tgaaattggt cctggaaaag ttttatctgg cttaattaaa aaaataaata
    14941 gagatgttaa gttaacatca attcaaactt tagaagatgt gaaaggatgg aatgaaaatg
    15001 actaagagtg ctttagtaac aggtgcatca agaggaattg gacgtagtat tgcgttacaa
    15061 ttagcagaag aaggatataa tgtagcagta aactatgcag gcagcaaaga gaaagctgaa
    15121 gcagtagtcg aagaaatcaa agctaaaggt gttgacagtt ttgcgattca agcaaatgtt
    15181 gccgatgctg atgaagttaa agcaatgatt aaagaagtag ttagccaatt tggttcttta
    15241 gatgttttag taaataatgc aggtattact cgcgataatt tattaatgcg tatgaaagaa
    15301 caagagtggg atgatgttat tgacacaaac ttaaaaggtg tatttaactg tattcaaaaa
    15361 gcaacaccac aaatgttaag acaacgtagt ggtgctatca ttaatttatc aagtgttgtt
    15421 ggagcagtag gtaatccggg acaagcaaac tatgttgcaa caaaagcagg tgttattggt
    15481 ttaactaaat ctgcggcgcg tgaattagca tctcgtggta tcactgtaaa tgcagttgca
    15541 cctggtttta ttgtttctga tatgacagat gctttaagtg atgagcttaa agaacaaatg
    15601 ttgactcaaa ttccgttagc acgttttggt caagacacag atattgctaa tacagtagcg
    15661 ttcttagcat cagacaaagc aaaatatatt acaggtcaaa caatccatgt aaatggtgga
    15721 atgtacatgt aatatatttg agctaaagct cattgacgca gtggttgact ggtcatccaa
    15781 tggagaaatg cctgacctag tcaactttgc gggggaaatt ctaagcaacc aagatatggt
    15841 tccagaattt ctccctaaga aacagtaatc aataaattga taactaataa gcggaacgcc
    15901 tgagctaaag ctcatgcata agaaacagta atcaataaat tgataactgt ttctaaaatt
    15961 tctacttgtt ttttagaatt taaaatggga aaatataata gtctatgtat aggcattttt
    16021 aaaggaggtg aatcgacgtg gaaaatttcg ataaagtaaa agatatcatc gttgaccgtt
    16081 taggtgtaga cgctgataaa gtaactgaag atgcatcttt caaagatgat ttaggcgctg
    16141 actcacttga tatcgctgaa ttagtaatgg aattagaaga cgagtttggt actgaaattc
    16201 ctgatgaaga agctgaaaaa atcaacactg ttggtgatgc tgttaaattt attaacagtc
    16261 ttgaaaaata ataaatctta catctgggtc gtcagtattg tcgactcagt ttttttcttt
    16321 aattatcaat agttttaacg taaaattaaa gatgattcaa gagcaacaca taaaggagat
    16381 aaaataatgt ctaaacaaaa gaaaagtgag atagttaatc gttttagaaa gcgctttgat
    16441 actaaaatga cagagttagg ctttacttat caaaatattg atttatacca acaagcattt
    16501 tcgcattcga gttttattaa tgattttaat atgaatcgtt tagaccataa tgagcgttta
    16561 gagtttttgg gtgatgcggt attagaattg acggtttcac gatatttatt tgataaacat
    16621 cccaacttgc cagaagggaa tttaacaaaa atgcgtgcca ctattgtatg tgagccctca
    16681 cttgtaatat ttgcgaataa aattggattg aacgaaatga ttttacttgg taaaggtgaa
    16741 gagaaaacag ggggacgtac aagaccatca t
//
LOCUS       DAMZPP010000057        16757 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMZPP010000057 DAMZPP010000000
VERSION     DAMZPP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16757
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.057"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..286)
                     /locus_tag="POH73_001382"
     CDS             complement(<1..286)
                     /locus_tag="POH73_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDI6887343.1"
                     /translation="MKKFIGSVLATTLILGGCSTMENESSKDTNTETKSVPEEMEASK
                     YVGQGFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGD"
     gene            complement(538..2289)
                     /gene="srrB"
                     /locus_tag="POH73_001383"
     CDS             complement(538..2289)
                     /gene="srrB"
                     /locus_tag="POH73_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HDI6887344.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAVREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNTRFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWTYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            complement(2270..2995)
                     /gene="srrA"
                     /locus_tag="POH73_001384"
     CDS             complement(2270..2995)
                     /gene="srrA"
                     /locus_tag="POH73_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HDI6887345.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            complement(3128..3865)
                     /locus_tag="POH73_001385"
     CDS             complement(3128..3865)
                     /locus_tag="POH73_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDI6887346.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            complement(3858..4400)
                     /gene="scpB"
                     /locus_tag="POH73_001386"
     CDS             complement(3858..4400)
                     /gene="scpB"
                     /locus_tag="POH73_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDI6887347.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDISKDQLVELIENYSS
                     HGLIIQRFGTTYVLTTKKEASMYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            complement(4393..5124)
                     /locus_tag="POH73_001387"
     CDS             complement(4393..5124)
                     /locus_tag="POH73_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDI6887348.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDIDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTELSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIVRGVNYHFG"
     gene            5216..5722
                     /locus_tag="POH73_001388"
     CDS             5216..5722
                     /locus_tag="POH73_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HDI6887349.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEKQIAKLNA
                     AKPFSSVILPITPDFERIIKANYPDYDYNHETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            complement(5800..6687)
                     /gene="xerD"
                     /locus_tag="POH73_001389"
     CDS             complement(5800..6687)
                     /gene="xerD"
                     /locus_tag="POH73_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDI6887350.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(6736..7185)
                     /locus_tag="POH73_001390"
     CDS             complement(6736..7185)
                     /locus_tag="POH73_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDI6887351.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            complement(7290..7832)
                     /locus_tag="POH73_001391"
     CDS             complement(7290..7832)
                     /locus_tag="POH73_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDI6887352.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCALTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNVEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            7914..8822
                     /locus_tag="POH73_001392"
     CDS             7914..8822
                     /locus_tag="POH73_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6887353.1"
                     /translation="MQKNILKSGIALSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALFKYQQRDDIVIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNDDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            complement(9038..9286)
                     /locus_tag="POH73_001393"
     CDS             complement(9038..9286)
                     /locus_tag="POH73_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887354.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILSDISVDQISDSTL
                     YYTCEKFKLLVLQGNVLGVQVITNNDELTCEVKYRKMF"
     gene            complement(9302..10057)
                     /locus_tag="POH73_001394"
     CDS             complement(9302..10057)
                     /locus_tag="POH73_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6887355.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     DQELGKTINVIPCDLNDLKSIQALQFEDNTLFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGKIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            10199..11014
                     /gene="proC"
                     /locus_tag="POH73_001395"
     CDS             10199..11014
                     /gene="proC"
                     /locus_tag="POH73_001395"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDI6887356.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
     gene            complement(11119..12039)
                     /gene="rnz"
                     /locus_tag="POH73_001396"
     CDS             complement(11119..12039)
                     /gene="rnz"
                     /locus_tag="POH73_001396"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDI6887357.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPIVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKI"
     gene            12352..13836
                     /locus_tag="POH73_001397"
     CDS             12352..13836
                     /locus_tag="POH73_001397"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDI6887358.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     HHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            14066..14932
                     /locus_tag="POH73_001398"
     CDS             14066..14932
                     /locus_tag="POH73_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDI6887359.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(15015..16664)
                     /locus_tag="POH73_001399"
     CDS             complement(15015..16664)
                     /locus_tag="POH73_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDI6887360.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDLG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEDSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDISHGEVKLFNYDAKVNLKQLLPYEACVIELN"
BASE COUNT         5400 a         3039 c         2437 g         5881 t
ORIGIN      
        1 cgtcgccact acctacaaca ttggtagctt taacttttaa accgaagtta tccataaaaa
       61 attgttcgcc tcgtttggca attttatctt tatgcttctt cgcaaattca atcgcatctt
      121 tttctgcagg cggttggaag ccttgtccta catattttga agcttccatt tcttctggta
      181 ctgattttgt ttctgtattc gtgtctttac ttgattcatt ttccatcgtg gaacatcccc
      241 ctaaaattaa tgtcgtagct aaaactgatc caatgaattt tttcatatga cacctcgaat
      301 aatttaatta ttacaataca tctatatttt accctatttt tataatcaaa taaataactt
      361 tatcaaactt tatattttta tataaattag taatatattt aatagttgat attcgcaaaa
      421 accctaaacc ctaaatccta atccacatac aaaactcccc acatcaataa aaaaacgcat
      481 gctataaatt agagtctcta atcaataaca tgcgttctga aataactata ttcaatttta
      541 ttctggtttt ggtagtttaa taataaatgt tgtgcctttc cctaattcgc ttttaacatc
      601 tatggaacca ccatgctctt cgataatcat tttacaaatg aacaaaccta aaccggtacc
      661 ttgtttacct cgcgttctcg ctgcatcaac tttataaaaa cgatcaaata cttgttgtaa
      721 atgttctggt gcaatgcctg tacctgtatc tttaatgtat aaaatatctt cgctttcatt
      781 ttcatcacaa gtaattgcaa tttcatcacc aggtttcgta taacgtgatg cattatcaat
      841 taagttcgtt agtacttggt ccatgcgatc catatcataa gtccaaacac gcttcttaca
      901 ataattaaaa gtcatattta gacctaaatc atcagcctgt tgacgatact taattttcat
      961 cttatctagt aacgctgcaa taggctgaac ttctttattt acggataacc cttcagcatc
     1021 catgcgagcg acatttaaca attcattaac taaacgattt aaacgtttcg attcatcaag
     1081 gacaatggca agcgattctt ttatttcatc cggttctgta acaataccat ctacaattga
     1141 ttcagtataa ccttgaagta atgatatcgg tgtacgtaat tcatgtgata cattagcaat
     1201 gaaatctttc ttcatttgat ctagattgtg ctcattagtc atatcacgaa ctgtcacaac
     1261 aacaccactt ttacctcctt gttcaatctt gtcgatatag cttgtggtca caacaaagaa
     1321 tcgtgtattc atttctaaat cacgcatttc cgtttgtttt gatttaaaag tatcttcaat
     1381 ttgtcttaat aagaaagctt tagcatcttc atcaatattg tccataatat cattcgccat
     1441 cttattcgat aagataattt gtcgactctc attaatacct aggacacctt ctaccataga
     1501 gttaattaag ctgtctctaa tatttttaga tgtggataat gcgtcgacat gctcttcgat
     1561 ttctgtactc atctgattaa atgcctgtga taattgacca atttcatctt tcgttgtgac
     1621 agaaggttta taagagtaat ccccttcaga tacacgtgta gcttggtctc ttaaacgtct
     1681 taaaggtttt gtaattcttg acgataagaa aaacgcaaag actgttgtaa ttgttaagaa
     1741 aataacagcc gtaattatag tgataatcgt aatagcatta tttgtatctt cgattgattt
     1801 caagtcttta tatataaaga ctccactata tttgctatga ctattcttct gtgcttttgt
     1861 tggatagcct aacaaaatat atgtttgaga tgagcccttt tctttaatcg ttacatttcg
     1921 agtaacagat ttacctttat caaacacatc gtcaaaatgg tcgttgttga ctacttcatt
     1981 caacatttgc tttttaatat tagaaagcga agccgttgat tgacgatgtt tattatttat
     2041 aatcatcaac ccaccaggat tttcaattaa tgtttgacta tattttattg cttcttcttt
     2101 attatgtgat tgttcgacca gtgaacttat acgtctagca tcttctctta cggcattttc
     2161 agtttcttgt gtgaaatagt attgcataaa ggtaattaaa gcaatactta ataaaattaa
     2221 aactgtcgtc actattaaaa taatagttaa ccacagttta attacgacac tatttagccg
     2281 gctcatcatt agatttaacc tcaaatttat acccaacgcc ccagactgtt tgaatcatat
     2341 gcgcagcttc gctagacaca cgatttaact tttctctaag tcgtttaaca tgagtatcaa
     2401 ctgttcttaa atcaccatag aattcataat gccaaacttc ttttaataat tgttcacggt
     2461 caaatacttt atttggtgtt ttagctaaat atattaataa ttcgtactct ttaggagtca
     2521 aattaacttc ttgattatca gcaagtacgc gatgtgcatc attatctatt tctaaatgtt
     2581 taaattcaat cacatcacgt gcgtgaggtt cgctttgttc tacagttgta gattgcgttc
     2641 ttcttagaag tgctttaact cttaagacta cttctcttgg tgaaaatggt ttgacgatat
     2701 aatcatctgc accagactca aaaccttcaa cacggtttgt ttcttcacct ttagcagtca
     2761 acataataat cggtgtttgt ttatgttcac gcaatttagt tgccacctgg ataccatcca
     2821 tttcaggcaa cattaaatct agtagtatgc aagcataatt attctccatt gcaagttcat
     2881 aagcctcttg gccattactt gcctcatgga tttcaaaaga ttctctttct aaatacattt
     2941 taagtaacct tctgattcta tcctcatcat ctacgataag tatttcgttc gacatacagg
     3001 tcatacctcc cacacatgct tttctttaca aaagtattat atcacaggaa agcgcataca
     3061 gttattaaaa acctaaaatt ttgtgaaaaa attctcaaac tttgtgataa atacatattt
     3121 tatttaatta tttcccatgt tctgctaaat gacgcatcac ttttacttca tgaggcgtca
     3181 atacacgtcc ttcaccagca ttcaaaccga caacatttaa aggcccatat tcaatacgag
     3241 acagtttcgt cacttgatga ccaaaatgtt cgaacattct tctgacttga cgattacgac
     3301 cttctgttat tgtaatttca accaatgttg tgtttttatc tttatcttgt ttcttaactt
     3361 tcacttcagc cggttgcgtc ataccatctt ctaattcaat acctttttct agcgctttca
     3421 cttcttctct cattaagtaa ccttttaatt ttgctacata tttcttctta atttgatatc
     3481 ttggatgtgt cattaaatta gtaaattcac catcatttgt aagtaataac aatccagaag
     3541 tatcatagtc taaacgacca acaggataaa tacgcgcttc tatatcttta aagtaatctg
     3601 taaccactgt acgtcctcta tcatcagata cacttgttat cacttgagtt ggtttatgga
     3661 ataaaatgta aattttgtct tctagctcta ttttaatacc ttcaacttca atcgtatctg
     3721 atggcttcac ttttgttcct aattcagtga cagtcgtacc attcactttc acttttcctt
     3781 cagaaattaa agtttctgct ttacgtcttg aagtataacc gctattagct atacgttttt
     3841 gtaatcgttc taattcttta gtcattattt tctccttttt gattgactag attactgaaa
     3901 aaagcatcca tttcttcatc gtcttcttca gttgtcggca aatcttctat attggaaata
     3961 ccaaatacat ttaaaaataa atcagtagta attaattgtt ggctacgctg ttcattgacc
     4021 actttagcct caactagtcc tttggcaatc aatgtcttga ccgcaccatc tgaattgata
     4081 ctacgaatta attcaatatc acttcttgat aatggttggt tataagcaat aattgatagt
     4141 acttccattg ctgcttgtga taatttcatt tgtgactttt gttcaattaa ttgttcaata
     4201 tacattgacg cttctttttt agtcgttaaa acatacgtcg ttccaaatcg ttgtattatt
     4261 aatccatgtg atgaataatt ttcaattaat tctacgagtt ggtctttcga tatatccaaa
     4321 atttctaata gctgtttttc atctaaacct tcatcgccag ctgtaaataa tagcgactct
     4381 aatataccat gattatccaa aatggtagtt cactcctcta acaatgttaa tatcttcaaa
     4441 attacgttgt tgctcaatat taattattcc tgcttttgac atctctaaaa tagctaaaaa
     4501 gtgagtgact acttgttcaa ttggctcaga aaacgtaaac agactaaaga agttaaaatg
     4561 atctttatct ttcaatctcg atgtcacttg ttctgtagct tgttgaatgg taaatgtctc
     4621 ttttcggatt tcaacagatt taggtgtatt taactcaact ctatttttaa ctctttgata
     4681 agctacaatt aattcagtta agtcaatcgt atgatttgga tcccaagatt catctgtttc
     4741 caaatgagat aactctgtcg gtctttttgt aaaataaaaa tctctttctt ctttcatgtc
     4801 atttaaaata gcagtatatt ctttataatt ttgatattct attaaacgcc caactaaatc
     4861 ttcccgtggg tcatcatcaa catctatatc tgatgttgat tgtggtaata gcatcttact
     4921 tttaatcatt aagagttctg acgctaatac taggtattca cttgcaatat taatttcaag
     4981 ctgtttcatt gcatgaacgt actgcatata ctgctctgtt aatgcttgca taggaatatc
     5041 ataaatatct atttcaaatt tttggataag atgcagtaat aaatctaatg gtccattgaa
     5101 agcatctaat ttaacttcat acataatatc tacctcgtat tgcgtcatat gtgcataatt
     5161 ctctaaaata ttatagcatg aaaataactt acttttaaat tggggtgata acaaaatgca
     5221 acaggcactg ataaatttct attatcaatt tcatactaag caacattatt tcctatgtca
     5281 tgacatttta gaagatgcat ggaaagctga aaataactac agtaagcaag atgcagttgt
     5341 tagtttaatc ttgtttgcaa ctgcttgtta ccattatcgc cgcaataatt taaaaggcgc
     5401 ctataagtct tttaataaat ctaaagaaat aatacaaaat gctaaagata gagatgcgtt
     5461 ataccttaat ttaaatgatt atcagttgtt aattgagaaa cagattgcaa aattgaatgc
     5521 agcaaagcct ttttcatctg ttatattacc aataactcca gactttgagc gcatcatcaa
     5581 ggccaactat ccagattatg attataatca cgaaacagca acagatcctt ttattgtaga
     5641 ccatcacatg cggcgtgatc gttcagaagt gattgcagca aaagaagaag cgattcaatt
     5701 aagaaaacat agacgtaatt aatcaaccat aactcaaaag ccacaatagc acagacattt
     5761 ttagtcatgt gtattgtgac ttttgagtta tggttcactt tatgctctag gatgaaattg
     5821 gttatacatt tttctaattt gagattttga aacatgtgta tagagttggg tagtagatat
     5881 gtcagagtga cctaacatct cttgcactgc tcttaaatct gcgccatttt ccaataaatg
     5941 tgtcgcaaaa gaatggcgca acgtatgtgg cgttagcgtc tttttaatgt ttgcctttac
     6001 accattttgt ttaatcattt tccatattgc ttgtcgtgat aaaggtttac catgcatatt
     6061 taaaaataag acttcagtaa cagtcttttt taaaagttgc ggtctaatcg tttcaatata
     6121 agtagttaag tactcaatga ctgcgtcgcc taatggcaca attctttctt tatcgccttt
     6181 accaaatacg cgtacaaacc ccattattaa gttcacgttc tctagctcta aatgtatcaa
     6241 ttcagataca cgcattcccg ttgcgtacag aagttctaac atcgtacgat cacgataacc
     6301 attaatttta tttaaatccg gcgtttctaa taaagccaac acttcgtcaa catttaaaac
     6361 gtcaggcaat tttttgtcat attttggtga atctaataat accgttggat ctttcgccgc
     6421 atatttttct cttatagcaa attgatgaaa actacggatt gttgaaataa atcgcgcaat
     6481 ggatttagca gattgccctt ggtctattaa atgccccaaa cactcttgaa ttaattgtcg
     6541 atctataaaa tcaatatgcg agatatgatg ttcagtcata taatcttgat actttttcaa
     6601 atcacgtcta taagcaccaa ttgtgttaga acttagtcct ttttcaattt gtataaaacg
     6661 caaatattct tcaataattg tttccataac cacacctcta aaaaaagcga aggttaattt
     6721 aacctacgca atttactatc ctttaccttt agcttggcac gtttcacaca caccatggaa
     6781 agttaaacga tgatctaaaa ttttaaaatt gaactcattt tcaactcgat tttcaacttc
     6841 tggtaacaaa tcttcatcga tttcatctac acgaccacat tccatacata ctaaatgatg
     6901 gtggaaatgt tttgcgcctt cttttcttaa atcaaaacga gcgacgccat caccaaagtt
     6961 aattttgtcg acaactttta gttcagctaa caactctaac gttctgtata ctgtcgccaa
     7021 gccaatttca ggcgctttgt cttttacttt cagatatacg tcttcagcac ttagatgatc
     7081 tttttcattt tcaattagaa ctctaacagt agcttcgcgt tgtggcgtta gcttatatga
     7141 tgattgttgt aattgttgct taacgcgatt taatcgttct tccaacgatg tccactcccc
     7201 tactaataat taaaatcatt acaaattatt tcaaacttca caattaaaac taacagtttt
     7261 ctcaataaaa tgcaagcttt tctcatttgt tatttagaat gattataatt taataatagg
     7321 tgttgcaatg caatgatagt cttcgcatct tctatttctt tattcattag catcgattta
     7381 acattctcaa tcggaacttt aatgacttcg acaaattcat cttcatctaa gtgaactgtg
     7441 ccttcttcta cattatccgt aaaatatatt gataattgtt catcacaaaa acctggtgaa
     7501 ccatacatat ctacaacatg tgttaactct ttggcaatat atcctgtttc ttcttctaat
     7561 tcacgcttag ccgcttcgac tctatcttca tcatcttcta atttaccagc tggaatttct
     7621 aacaatggtt tttctactgg tttacgatac tgtttcacta atacgacttc ttttttaggt
     7681 gtcaatgcac aaacagcaac tgcaccatta tgataaacta attctcttgt tgacgtttca
     7741 ccgtttggta atgtcactgt atgaatttct acatctacaa ttttgccatt ataaataact
     7801 gttcgatcaa ttgttttttc atttaaatcc attataatca cgttccttta aattcatatt
     7861 atatattgat acactatgct tgttagctaa agtgtatcga aaggagaaca gacatgcaaa
     7921 aaaatatatt aaaaagtggt attgcattat ctgaattagg tctaggttgc atgagtttag
     7981 gtacagattt aaaaaaagcc gaacaaatta tagattgtgc tgttgaaaat ggtatcactt
     8041 attttgatac agcagacatg tacgataaag gtataaatga atcagttgtt ggtaaggcac
     8101 tttttaaata tcaacaacgc gatgatattg tcattggtac aaaagtaggc aatcgtttaa
     8161 caaaagatgg cagtacaaca tgggatccga gtaaatccta tattaaagag gcggttaaag
     8221 gttcactaaa gcgtttaggt attgatcata tcgatttata tcaacttcat ggcggaacca
     8281 ttgatgatcc attagacgaa acaataagcg catttgatga attgaaacaa gaaggaatta
     8341 tacgtgctta cggtatttct tctattcgcc caaatgtaat tgattattat ttaaaacata
     8401 gtcaaatcga aacgataatg tctcaattca atttgattga taatcgtcca gaatcattat
     8461 tagatgcaat tcacaacgat gatgttaaag tattggcaag aggacctgtg tctaaaggat
     8521 tattaacttc aaacagtgtt aatgtgctcg acaataaatt taaagatggt atttttgatt
     8581 attctcatga tgaattgggt gaaacaatag cctctattaa agaaattgaa agtaatttat
     8641 ctgcattgac atttagttat ttaacatcac atgacgtgct tggttccatc attgtaggtg
     8701 caagtagcgt cgaccaatta aaagaaaata ttgaaaacta tcatactaaa gttagtttag
     8761 atcagattaa aacagcaaga gctcgtgtaa aggatttgga atataccaat catttagtgt
     8821 agaagtcatt ttcagtaata aaaacagcag tatgaggcgt ttcattataa aaatgcctta
     8881 ctgctgttgt ttatgtaaaa ttcgctataa tttatgatta agattgctca cttataatag
     8941 taattaaaga gttgtcctta cgcatcagta tttagtaatt ccgccctgcg gcagtgcctt
     9001 aagcaaactt ctgccacttc atcttttaat aattttatta aaacatcttt ctatatttca
     9061 cttcgcatgt taattcatca ttattagtta ttacttgtac acccaacaca tttccttgca
     9121 acacaagtag tttgaatttt tcacaagtat aatataatgt actgtctgaa atttggtcta
     9181 cagaaatatc gcttaaaata tccagcactg taaattcttc aaatactgat agttgttccg
     9241 catatcgtac acaaagtctt accacactct ccgattgaca gttcattgcc atcccaccta
     9301 tttatgcttt atttttaaat aatttaggga aacatcgttc aaaaaatcta ggcgcaattt
     9361 gatacatttt caacgcatga tgcatccatt taggccgatt aatttccaat tgttttgttt
     9421 taatgccata aatgatatct tctgcaagct gattagcatc aagcataatt ttccccatct
     9481 ttttagcata cttcattgat gggtcggctt tttgatgaaa aggtgtatca atcgggccaa
     9541 cattaactgt catgatatgt aagtttggtg actctagtct taaagcattc attaatgcat
     9601 aaaaccctgc tttcgatgcc ccataatgtg cagcatttgc ttgtgtagaa aatgcagctt
     9661 gacttgaaat acctacaata tgtgcgttag atgttaaata tggtctcaac acagtatata
     9721 aaacattaaa actaattaaa ttaagctgat acgtttcgat catttctgaa aaactatggt
     9781 ctgaaataga tttgaaataa cctaaacctg cactataaat gaatccatcg aataatgtat
     9841 tgtcttcaaa ttgcagtgcc tgtatcgact tcaaatcatt taagtcacaa ggaataacat
     9901 ttatagtttt ccccaattcc tggtcaaaga ttctagttgc tttatcaaca tcacgcacca
     9961 acaacgttac atgcacttta ttttctagta actttcggac aatcgataaa cctaaaccac
    10021 tcgtaccacc agtcactata aaatgttgtc ctttcatcaa ttaaccttcc ttttccatta
    10081 tatagaatgc aatttatcaa ctttacataa ttgagacaag ttgattatct ttcctaatat
    10141 atatacaata ataagaaaat ataacataca aatcaaaaac taaagggatg tgacgttaat
    10201 gaaactcgta ttttatggag ctggtaatat ggcacaagct atatttacag gaattattaa
    10261 ctcaagcaac ttagatgcca atgatatata tttaacaaat aaatctaatg aacaagcttt
    10321 aaaagcattc gctgaaaaac taggtgttaa ctatagttat gatgatgcga cattattaaa
    10381 agatgcagat tatgtatttt taggtaccaa accacatgac tttgatgctc tagcaacacg
    10441 catcaaacca catattacaa aagacaattg cttcatttca attatggcag gtattccgat
    10501 tgattatatt aaacagcaat tagaatgcca aaatccagtt gctagaatta tgccaaacac
    10561 aaatgcgcaa gttggacact cagttactgg cattagtttt tcaaacaact ttgaccctaa
    10621 atctaaagat gaaattaacg atttagttaa agcatttggt tcagtaattg aagtatcaga
    10681 ggatcattta catcaagtaa cagctatcac cggaagcggc ccagcatttt tatatcatgt
    10741 attcgagcaa tacgttaaag ctggtacgaa acttggacta gaaaaagaac aagttgaaga
    10801 atctatacgc aaccttatta taggtacaag taagatgatt gaacgttcag atttgagcat
    10861 ggctcaatta agaaaaaata ttacctctaa aggtggtacg acacaagctg gccttgatac
    10921 attgtcacaa tatgatttag tatctatttt cgaagattgt ctaaatgctg ccgtcgaccg
    10981 tagtattgaa ctttctaata tagaagacca ataaaaacaa acccgccaac acatgtatgc
    11041 atcatcgcaa gcactgtgtt tgacgggtta tttttataaa tttttactat ttggcaagca
    11101 ttgtttatta ctttgtcatt agattttaaa actatcaaaa tctttcacaa aattaaaatt
    11161 cggcgtatct tcattttgta tcaatgtttg ataaatttca tttatatctt ctgtattata
    11221 gcgattgctc aaatgtgtaa tcaacgtacg tttaacattg gcttctttta tcaatgcaaa
    11281 tacgtcttca atatggctat gatgataatt gttggctaaa tgcttttcac catctatata
    11341 ggtcgcttca tgtaccatca catcagcatc tctagaaatc acacgttcat tagaacatgg
    11401 ttttgtatca ccaaaaattg ctacaattgg accctgtttg gactcacctc taaaatcttt
    11461 tgattgataa acttgaccat tatgttcaaa tgtatcatga gattttactt cttgatattt
    11521 aggacctggt tcaagaccaa tgttttttaa cgcttcaaca ttgattgtac ctgtagtttc
    11581 aggtgccatt actctatatc catatgatgg aataccatga ttaagtaaat gcgcctctac
    11641 agtaaaacca tcatgatgat atgttagatg atcatcgatt tcaatatatg taattggata
    11701 gtttaaatgt gactctgata aattcataga catttccaca tatgctttaa ttccttttgg
    11761 tccaactaat gtaagcggct tttgttcacc gccctgaaaa gaacgactag aaagtaatcc
    11821 tggcaaacca aaaatatgat cgccatgcat atgagtaata aatatatgtg tcacttttcc
    11881 taatttaatt gcatgatgta aaatttggtg ctgtgtacct tcaccaacgt cgaaaagcca
    11941 tatggaattg gaatatggtt ctaaatttaa ggcgattgct tgtgtatttc tctcttttgt
    12001 aggcaaacct gcactcgttc caaaaaatgt aacttccata tatgcccctc cttttcttca
    12061 attcatttta tcataaaatt tgtatcaatt tagttttaat ttatacttga atcaaaatct
    12121 gttaaaaatt cgtctttgct agttaatttt tctaacttta acgtagacat aactatataa
    12181 attttgataa ttacgttata cttatcatta ataagtatca cattaaacat gatacatgaa
    12241 tcgatatttc atttaagaca ctgcatactg tcgagcatat tgaatgacct gctgaatgga
    12301 ttatcttata ataataaatc atatatctaa ttaagaattg aggttttaat cttgagtact
    12361 aaaaacaaac acatcccttg tttaatcaca atctttggtg caactggtga cttaagccat
    12421 cgtaagttgt ttccatcaat attccatctc taccaacaag acaatttaga tgaacatatt
    12481 gccatcatcg gtattggacg tcgtgacatt actaatgatg atttccgtaa tcaagtaaaa
    12541 tcatcaattc aaaagcacgt aaaagataca aacaaaattg acgcgtttat ggaacatgtc
    12601 ttctatcata gacatgatgt tagtaatgaa gaaagctatc aagaattatt agattttagt
    12661 aatgaattag acagccaatt tgaattaaaa ggtaatcgac tattctattt agcaatggca
    12721 ccacaattct ttggcgttat ttctgattat ctaaaatctt ctggtcttac tgatacaaaa
    12781 ggatttaaac gccttgttat cgaaaaacca ttcggtagtg atttaaaatc agccgaagca
    12841 ttaaacaatc aaattcgtaa atcatttaaa gaagaagaaa tttatcgtat tgaccactat
    12901 ttaggaaaag acatggttca aaatatcgag gtattacgtt ttgcgaatgc gatgtttgaa
    12961 ccattatgga ataacaaata tatttcaaac atccaagtta catcttctga aatactaggt
    13021 gttgaagatc gtggtggtta ttatgaatca agtggtgcgc taaaagatat ggtgcaaaac
    13081 cacatgttac aaatggttgc attattagct atggaagcac ctattagttt aaatagtgaa
    13141 gatatccgtg ctgagaaagt aaaagtactt aaatcactgc atcatttcca atctgaagat
    13201 gttaaaaaga actttgttcg tggtcaatat ggcgaaggct atatcgatgg taaacaagtt
    13261 aaagcatacc gtgatgaaga tcgcgttgca gatgactcta acacacctac atttgtttca
    13321 ggtaaattaa caattgataa ctttagatgg gctggcgtac cattctatat ccgtactggt
    13381 aaacgtatga aatctaaaac aattcaagtt gtcgttgaat ttaaagaagt accaatgaac
    13441 ttatactatg aaactgataa actgttagat tcaaacctat tagtaatcaa tattcaacca
    13501 aatgaaggtg tatctttaca tctaaatgct aagaaaaata cacaaggtat cgaaactgaa
    13561 cctgttcaat tgtcttattc aatgagcgct caagataaaa tgaatactgt agatgcatat
    13621 gaaaatctat tattcgattg tcttaaaggt gatgccacta actttacgca ctgggaagaa
    13681 ttaaaatcaa catggaaatt tgttgatgca attcaagatg aatggaatat ggttgatcca
    13741 gaattcccta actatgaatc aggtactaat ggtccattag aaagtgattt attacttgct
    13801 cgtgatggta accattggtg ggacgatatt caataattga attaaagcgc acatgttaaa
    13861 caaaaataaa tgagcgaatg acaaggattg attacgaatc aagtatatca attgttctat
    13921 cattcgcttt ttttctagac tatattatga aattatattt tacaatgccc aaaactattt
    13981 taataatcat tgaacaaatg ggtgtataat ttatagaaat aatgtagaat aaaaataaat
    14041 gattgaatta attggagtga aagttttgga cgttatcaag caaatacaac aggcaattgt
    14101 ttatattgaa gatcgtttat tagagccttt caatttgcaa gaattaagtg attacgttgg
    14161 tctttcgcca taccatcttg atcaatcatt taaaatgatt gtcggcttat ctccagaagc
    14221 ttatgcacgc gcgcgtaaaa tgacactcgc tgcaaatgat gtgattaatg gtgctacacg
    14281 acttgtagat atcgctaaaa aatatcacta tgcaaattca aatgattttg caaatgattt
    14341 tagtgatttt cacggcgtat cacctattca agcttctact aaaaaagatg aattacaaat
    14401 tcaagagcga ttatatatca aattatcaac tactgagaga gcaccttatc catacagatt
    14461 agaagagaca gatgatattt cattggttgg atatgcacga tttatagaca ctaagtattt
    14521 gtcacatcct tttaatgttc cggatttttt agaagacttg ctcattgatg gtaaaattaa
    14581 agagttacga cgatataatg acgttagtcc atttgaacta tttgttatta gttgtcctct
    14641 tgaaaatggt ttagaaatat ttgtaggtgt accaagtgaa cgttatcctg cacacttaga
    14701 aagtcgattt ttacctggca aacattgtgc gaaattcaat ttacaaggtg aaattgatta
    14761 tgcaactaat gaagcttggt actatattga atcaagtttg cagttaacat tgccatatga
    14821 acgaaatgat ttatatgttg aagtgtaccc tctcgatatt tcatttaatg acccattcac
    14881 taaaattcag ctttggattc ctgttaaaca gagtccttat gacgaagatt aaataattaa
    14941 aaacaaagaa gccccctaat aaatctatag gtctacaaat ggccttagat tctattaggg
    15001 ggcatattaa tatgttaatt tagttcgata acacatgctt catatggaag taactgtttt
    15061 aaattaactt tggcatcata attaaatagc ttcacttctc catgacttat atcaaatggt
    15121 acagttaatt ctgcttcgtg gttagtaaga ttacctacaa taagaacttg cttttcattt
    15181 aatgttctcg tgtacgcaaa aacttgtgaa ttttcagcat ctactaaatc aaattgacca
    15241 tatacgtata catcattaga ctttcttaat tgaattaaat ctttataaaa ttgtaatact
    15301 gaatcctcat cttctaattg ttgtgcaaca ttgatagttt tataattcgg attcactggg
    15361 aaccacggtt caccatttgt aaatcctcca tttaacgtat catcccattg cattggtgtg
    15421 cgagaattat ctcggttctc atctttatat ttcgcaagta aagcgtctac atctccacct
    15481 tgagctttca caatttgata gtcattttta acagcaacat cgttaaatgt ttcaatactt
    15541 tcaaatggat aattcgtcat accaatttct tgaccttgat aaatgaatgg cgtaccttgt
    15601 tgcaagaaat aaactgctgc atgacttgtt gctgattcat accaatactt gtcatcgtca
    15661 ccccacgtcg atacacgtcg tggttggtca tgattttcaa taaacaacgc attccaacct
    15721 ttattttcaa gttgtttttg ccatctattt aatacagact tatacgaatt tacatcaaag
    15781 tgagaatcac cactattcca cagtcccaaa tgttcaaatt ggaatatcat attaaattta
    15841 ccattttctt ccccgaccca gtcatcagca tcatcagggc ttacaccatt cgcttcacca
    15901 acagtcataa tgtcatactt acttaatgag cgatctttca tctcttgtaa ccaagtttgt
    15961 atacctggct gattcatatc tacatcaaat gctggggcat atgttttacc ctcaggtaca
    16021 ggtaagtcac ccgcttcaaa cgtcttctta atatgcgtaa ttgcatctac tctaaatcca
    16081 tcaatgcctt tatcaaacca ccagttcatc atttcaaata cagcatctct aacttccgga
    16141 ttaccccaat tcaaatcagg ttgtttttta ctgaatagat ggaaataata ttgctcagta
    16201 ttagcatcat attcccatgt agatccatta aatatacttt cccagttgtt aggttcagag
    16261 ccatctggct ttggatcttg ccaaatgtac caatcacgtt tgggattgtc tttactagat
    16321 ttggattcta taaaccaagg atgttcatca gatgtatgat tcacaactaa atctaaaata
    16381 agcttcatac ctctatcatg aacacctttt aataaacgat caaagtcttc catcgttcca
    16441 aattcatcca taatctcttg gtagtcacta atatcataac cattatcatc attaggtgat
    16501 ttaaacattg gactgagcca aatgacatcg atacctaaat cttttaagta gtccaattta
    16561 tcaatcattc caggtaaatc cccaatacca tcgtgattac tatcattaaa acttcttgga
    16621 tatacttgat atgctactgc ttctttccac cattgcttat tcattttaaa actcctttgc
    16681 tatcgctgtg ttgattttct tatttttaat tctgtatcta taatgacgag ttcaataaca
    16741 tcctgtgctt tattttt
//
LOCUS       DAMZPP010000058        16750 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMZPP010000058 DAMZPP010000000
VERSION     DAMZPP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16750
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.058"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            290..826
                     /locus_tag="POH73_001400"
     CDS             290..826
                     /locus_tag="POH73_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6887361.1"
                     /translation="MANTTIRLANENDAVALHQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVKYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEETFLRDTLKAAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            1054..1806
                     /locus_tag="POH73_001401"
     CDS             1054..1806
                     /locus_tag="POH73_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HDI6887362.1"
                     /translation="MNGNIIVAHGMRRGRQNQALEAFISELVKDDIHHYHIAFLESEH
                     QDLETVMTTLIQSGVKQLKIVPLLLFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMTEIIMQRIDEVRVDEMQQVGVVVVAHGNINGKFTKAHEELQTCVQHLQINKPT
                     YARTLYGEISFIHDLEDIARQYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHITP
                     SINFDPILKQIINDRLESLNDSNENIKALTKS"
     gene            1852..4257
                     /locus_tag="POH73_001402"
     CDS             1852..4257
                     /locus_tag="POH73_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6887363.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHDIKLITNDPVVDVDRANQNVTTANGIEVAY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPTDLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARKIFEV
                     EKV"
     gene            4261..4575
                     /gene="nirD"
                     /locus_tag="POH73_001403"
     CDS             4261..4575
                     /gene="nirD"
                     /locus_tag="POH73_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDI6887364.1"
                     /translation="METKEKIKVTTLDELTPLIGKKVIVKGKEIGLFLTESGTIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            4566..5543
                     /gene="cobA"
                     /locus_tag="POH73_001404"
     CDS             4566..5543
                     /gene="cobA"
                     /locus_tag="POH73_001404"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDI6887365.1"
                     /translation="MSVNGYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYSKSTTEIIDVGKKPYAKHIQQEKINECIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNNFNIAFEIVPGVTSASAAVATMQTGLTMRAVAKSVTFSTGYFKDSEENEVD
                     VNALVNGGTLAIYMGVKRLEKIIAQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNIV
                     GKLEHYAIEAKPGICIIGEVVGYTENVSTTSNPTQQFYVVSGSKPDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDTFIKQQENVTYISTDRADTNTVLCH"
     gene            5850..9539
                     /locus_tag="POH73_001405"
     CDS             5850..9539
                     /locus_tag="POH73_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDI6887366.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTDIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFIIMLDEDENGYKAGRFLRASDLGQTTEQSEWKPVIHDTISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESNGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILNISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNVERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            9529..11088
                     /gene="narH"
                     /locus_tag="POH73_001406"
     CDS             9529..11088
                     /gene="narH"
                     /locus_tag="POH73_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDI6887367.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGNHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGISYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTNQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTKPVKEALQRMAMMRSYMRSQVTQQPFDTSRLERLGLTERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            11081..11656
                     /gene="narJ"
                     /locus_tag="POH73_001407"
     CDS             11081..11656
                     /gene="narJ"
                     /locus_tag="POH73_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDI6887368.1"
                     /translation="MINFDNFKKYQESFGYIAQQLCFPEKLTFHPKTFEETISKDHPG
                     YDDLIAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            11649..12326
                     /gene="narI"
                     /locus_tag="POH73_001408"
     CDS             11649..12326
                     /gene="narI"
                     /locus_tag="POH73_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDI6887369.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKSWMDAAGVSEHLYHIGAVYIGSIFGIITLV
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNVQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKIHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            12346..12798
                     /gene="nreA"
                     /locus_tag="POH73_001409"
     CDS             12346..12798
                     /gene="nreA"
                     /locus_tag="POH73_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HDI6887370.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMIIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRSLPKIFDNDDIQRKFGIFNDDK"
     gene            12822..13856
                     /gene="nreB"
                     /locus_tag="POH73_001410"
     CDS             12822..13856
                     /gene="nreB"
                     /locus_tag="POH73_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HDI6887371.1"
                     /translation="MINEDSIQLEALLKKYYEHSIEKIVFADDSGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTAKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGVKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            13879..14532
                     /gene="nreC"
                     /locus_tag="POH73_001411"
     CDS             13879..14532
                     /gene="nreC"
                     /locus_tag="POH73_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HDI6887372.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTSDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(14786..15526)
                     /locus_tag="POH73_001412"
                     /pseudo
     CDS             complement(14786..15526)
                     /locus_tag="POH73_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(15505..16419)
                     /locus_tag="POH73_001413"
     CDS             complement(15505..16419)
                     /locus_tag="POH73_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDI6887373.1"
                     /translation="MYKINIIRSDSVYKNILKTPLNDRDSIFTKEILVPFKKKFEVQQ
                     IPIHNDAKQTMSAIQFLDAFQKSPKDLRMSDQMSIQYLNNDFWSNCEKYLKVAIDQFA
                     NYSISSQVSNYHFTVLLGDSQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTNWSRDNV
                     KIKNTIYNHLHLKHIFESMPFLYGDDINKLQGRSIVGLSHAAGYACGYHLVKYFLQKT
                     NIPIEVATTLPAQKIINEVNDFWHTHTL"
BASE COUNT         5779 a         2489 c         3431 g         5051 t
ORIGIN      
        1 cgcagtcagc tactgccaat ataacattgt agagtctagg acattatgaa ctgcacgctt
       61 caaagtagac attgaaaaat gaaagctttg aagcgtgcat atcattaatt catgtcctag
      121 acttttactt tgctttcaat aatgaaataa ttgatgatca catacttatc ttatatcaat
      181 gaggcatgtt atctgacaca tgtgctaaat gttggtacgc tataaaagat aagcatagct
      241 aaaattaaag atgattcatt taaaataaac agaaagtgtg tggattcaaa tggcaaatac
      301 gacgattcga ctcgcaaatg aaaatgatgc agtagcacta catcaattaa tgcatgaggc
      361 gtttacaccg ttgagagaac ttggcataga ctggccttcg gtaaatgcaa atttagatgc
      421 tgtgaaagaa aatatagata aaaatactac atttgtcatg acaattgatg atgaaattat
      481 atctacgatt acggttaaat atccttgggg aagtgtgaaa agcatttcgg gctatccttt
      541 tgtctggtgg tttgcgacaa atcctgagta tgaagggaaa ggctatggta gtcaattact
      601 tacttatgtt gaagaaacat ttttacgaga tacattaaag gcagctgctg taactttagg
      661 tacatctgca agattgcatc cgtggttact taaaatttat gaaaaaagag gttatgaaat
      721 atacagcgaa catgaaaatg atgatggcga tttaggtgtg atcatgcgca aagtattgat
      781 acccgagcgc tttgacgaaa caatattagg ccaaccacca ttttaagata ataatataca
      841 ctacaagcga ccgatatatt gatgcttgct caaataaatg ttgagtaggg attcatatat
      901 ggtcgctttt ttgatttaac aaaaagggaa aatttataag gaatatgcat gactacaggc
      961 atcagggaat tccctgattt tacattataa tgtgattctt ttcacaaaaa cgaaatgatt
     1021 tggtatacaa tacgtgtgaa agggttggta aatgtgaatg ggaatatcat tgttgcacat
     1081 ggcatgaggc gcggtcggca gaatcaagcg ttagaagcgt ttatatctga attagtaaaa
     1141 gatgacatac atcattacca tattgcattt ttagaaagtg agcatcagga tttagaaaca
     1201 gtaatgacaa cgttgattca aagtggcgtt aaacagctta agattgtacc tttgcttctc
     1261 tttagtgcca tgcactatct caaggatata ccgaatatcg ttcatgaaat gaaacgacgg
     1321 tatccagaca taaaggtaga agtaagcgag ccacttggca cacatccttt aatgacagaa
     1381 ataatcatgc aacgtattga tgaggtgcgc gtggatgaaa tgcaacaagt cggtgttgtc
     1441 gttgttgcgc atggcaacat taatggcaag tttacaaaag cacatgaaga gttacagaca
     1501 tgcgttcaac atttgcagat taataaacct acttatgcga gaacgctata tggtgagatt
     1561 agttttatac atgatttaga agatattgcg agacagtatc aaaaattaat tgtcgtacct
     1621 ttatttttat atgacggaag acttgttaac aaagtgaagc agcaaatgaa tgacatggtt
     1681 attaatacag atatacacat cacaccttca attaacttcg acccgatttt aaaacaaatt
     1741 attaacgata gattagaaag tcttaatgac tccaatgaaa acataaaagc actaacgaaa
     1801 agttaacacc tttttttgaa ataaatatta tgtagtaaag gggcatattt tatggcaaag
     1861 caaaaactag taatgattgg taacggtatg gcgggcattc gaacaatcga agaaatatta
     1921 gagcgtgcca atgatttata tgatattacg gtaattggta aagaacctta tccaaactat
     1981 aaccgcatca tgctttcaaa tattttacaa aataaaatga cagttgaaga aacaattatg
     2041 aatccatatg agtggtatga agaacacgat attaagttaa ttaccaatga tccagtagtt
     2101 gacgtggata gagcaaatca aaatgtaacg actgcaaatg gtattgaagt agcatacgat
     2161 aaattaatat ttgcgacagg atctaaagca tttgtcattc cagtgccagg ttcgacatta
     2221 ccaagtgtta ttggctggag aacaattgat gatactgaac aaatgatgaa cattgctaag
     2281 actaaaaaga aagcaattgt aattggtgga ggcttactag gtttagagtg tgcacgtggt
     2341 ttattagatc aaggtatgga agtaacggtg ttacatttag ctgaatggtt gatggaaatg
     2401 cagctagacc gtaaagctgg aaatatgctt aaagcagatc tagaaaagca aggtatgaag
     2461 tttgaaatgc aagccaacac aactgaaatc ttaggagaag atgatgttga aggtgttaaa
     2521 ttggctgatg gacgcgagat tccgacagac ttagttgtta tggcagtagg tattcgacca
     2581 tacacagaag tagccaaaga atcaggtcta gatgttaatc gtggtattgt tgtcaatgat
     2641 gtgatgcaaa caagtgatag taatgtatat gcagttggtg aatgtgcaga acataacggc
     2701 aaagtttatg gactcgttgc accactatat gaacaaggta aagtattagc tgatcattta
     2761 acaaataaag aaacgaacgg atacaaggga tcaacaacat ttacgtcatt aaaagtttct
     2821 gggtgtgacc tatatagtgc tggtcaaatt gttgaaaatg ctgaaattaa aggtattgaa
     2881 atatttaata gcgttgataa taactataaa aaaatctttt taaaagacgg taatgtagtt
     2941 ggtgcagtac tatatggtga tatcgatgat ggttcacgct tttataacat gatgaaaaaa
     3001 ggtgaatcta ctgaagacta cacactggta tcattgctta ctaaaggtgg agaagaggca
     3061 tcgctatcaa ttgctgatat ggctgatgat gaaacaattt gtggctgtaa tggtgtcgat
     3121 aaaggcacga tagtaaatgc aattacggaa aatggtttta ctacagttga agaagttacg
     3181 gctaaaacga aagcaggtaa ttcatgtggt aaatgtaaac cgcaaattgc tcaaatctta
     3241 cagcacacgt tgggcgatga ctttgttgct gctaaacctg caggtatatg tggttgtact
     3301 gatttgacac gtgatcaaat tgttacgcaa atacgagcga aaggtttaaa aacatctaaa
     3361 gaagttcgac atgttttaaa ctttaaaaac aaaggtggat gtccaaaatg tcgacctgcg
     3421 attaactatt atttgaatat ggtttatcca catgatcatg aagatgaaag agaatcaaga
     3481 tttgctaacg aacgttacca tgcgaatatt caaaatgatg gtacattttc tgttatacct
     3541 caaatgcgtg ggggtgttac agatgcagac caactgattc gtctaggaga agtggctaag
     3601 aaatatcatg tgccactagt taaagtgaca ggttcgcaac gtgttggttt gtatggagtt
     3661 aaaaaagaag aattaccaaa tatatgggaa gacctaggta tgcgttcagc atcagcttat
     3721 ggtaagaaaa cacgctcagt taaaagctgt gttggtaaag agttttgtcg atttggtacg
     3781 caatacacga cacgacttgg cattcgttta gaaaaaacat ttgaatacat cgatacacct
     3841 cataaattca aaatgggtgt atcaggctgc ccaagaagtt gtgttgaatc aggtgttaaa
     3901 gattttggta ttatctcagt tgaaaatgga ttccaaatct atatcggtgg taatggtggt
     3961 acagaagttg aaaaagctga atttttaaca actgtagaaa cggaagatga agtaatcaaa
     4021 ttatgtggtg ctttgatgca atattatcgc gagacaggta tttatgctga aagaacagca
     4081 ccgtggttaa gaagactagg gtttgaaaat gttaaagaag tgttgcttga tccagaaaga
     4141 cagaatgaat tatttgaacg tattatggat gctaaaaaag cagtcgaagc tgaaccttgg
     4201 gaagcaatca ctagtaatgc gcaagctcga aaaatctttg aagtggagaa ggtgtaatac
     4261 atggaaacga aagagaaaat caaagtgaca actttagatg aattgacacc cttaattggt
     4321 aaaaaggtta ttgtcaaagg caaagaaata ggattgtttt taactgaaag tggtacaatt
     4381 cacgcgattc acaatatttg tccgcataaa caagggccat tgtcagaggg aacagtgagt
     4441 ggtgaatatg tattctgtcc actccatgat caaaagattg atttaaatac tgggattgta
     4501 caagaacctg atgaaggttg tgtagatgta tatgaggtag aagttacaga cgggaacgta
     4561 tatatatgtc tgtaaatggt tacggcaagg tttacttgat aggtgcgggc ccaggtaatc
     4621 cgaattattt gactaaaaag gctgagcgtt taatacgcga agcggatgtt atactatatg
     4681 atcgattagt gaatccgttg attttacaat attcgaagtc gacaactgag attatagatg
     4741 ttggtaaaaa gccatatgca aaacacattc agcaagaaaa aataaatgaa tgtatcgtgg
     4801 aagcagcacg tcgatataac aaggttgtga ggcttaaagg tggcgaccca gcaatatttg
     4861 gacgagttca agaagaagtt gacacactaa acaactttaa tattgcgttt gaaattgtac
     4921 ctggtgtgac atctgcgagt gctgcagttg ctacgatgca aacaggatta acgatgcgtg
     4981 ctgttgctaa aagtgttaca ttttcgacgg ggtattttaa agattcagaa gaaaatgaag
     5041 tagatgtcaa cgctttagta aatggtggta cgttggcgat atatatggga gttaaacgtt
     5101 tagagaaaat tatcgcccag atacaacagt acacagatat tgattatccg atagcgattg
     5161 tttttcaagc atcttgtttt aatgaattcg tcgttaaagg gcgcttaagt aatattgttg
     5221 ggaaactgga gcattacgca attgaggcaa aaccaggtat atgtattata ggcgaagtag
     5281 tgggttatac agaaaatgtc tcaacaactt ctaatccgac tcagcaattt tatgtagtaa
     5341 gtggttctaa acctgacgct cttatgctct gtgaacattt atatgacgaa ggttatggct
     5401 gtttgctaaa cccaaatgat acatcgaatg gtacatatca ttcgtcgcaa tatgattact
     5461 atgatacgtt tattaagcag caagaaaatg taacatatat ttcaaccgat cgtgcagata
     5521 ctaatacagt gttatgtcac taatttataa aaataaatga ataagtaagg tttcaaccga
     5581 gagaatatat tcgtgttgaa gccttatttg tcgttgtgcc aaatttgaac gatttagatt
     5641 ggcaataagc atgaccacac atgattgtgt attatttcaa tgaaatcccg ttattgatag
     5701 ggaaattcct taagtaatta ggtaattccc taatatttta tatttgtgaa aaaatgtaca
     5761 atctaattaa agcaatagtc ttgggcattt ttagtattta aaaacacgac atctacacat
     5821 cgtattttta tacgtaggag gatataaata tgggaaaatt tggattgaat ttctttaagc
     5881 caacagaaaa atttaatggg aattggtcga tcctagaaag taaaagtaga gaatgggaaa
     5941 aaatgtacag agaacgttgg agtcatgata aagaagtaag aacaacacat ggtgttaact
     6001 gtactggatc atgttcttgg aaagtctttg tgaaaaatgg tgtgattacc tgggaaaatc
     6061 aacaaactga ctatccaagt tgtggtccgg atatgcctga atatgaaccg agaggctgtc
     6121 ctcgtggtgc atcattctct tggtatgaat acagcccact tcgaattaaa tatccatata
     6181 ttcgtgggaa actttgggat ttatggactg aagcattaga agaaaacaat ggcaatcgtg
     6241 tggctgcatg ggcttctatt gttgaaaatg aagacaaagc caaacagtat aagcaagccc
     6301 gtggtatggg tggacatgtg cgttcaaatt ggaaagatgt tacggatatt atcgcagcac
     6361 aattactata tacaattaaa aaatatggtc ctgacagaat tgccggattc acaccgattc
     6421 cagcgatgtc tatgattagc tatgcagcag gtgctcgatt catcaattta cttggtggtg
     6481 aaatgcttag tttttatgac tggtatgcag atttaccacc ggcctctcca caaatttggg
     6541 gagaacaaac agacgtacct gaatcaagtg actggtataa cgcatcatac attattatgt
     6601 gggggtctaa tgtaccacta acgcgtacgc cagacgcaca ttttatgaca gaagttcgct
     6661 acaaaggaac aaaagttatt tcagtagcac ctgattatgc agaaaatgtg aagtttgctg
     6721 ataactggtt agcaccgaat cctggttcag atgctgcaat tgctcaagca atgacacatg
     6781 ttattttaca agagcattat gttaatcaac cgaatgaacg ctttattaat tatgcgaaac
     6841 aatatacaga catgccattt atcatcatgt tagatgaaga tgaaaatgga tataaagcag
     6901 gtcgattttt aagagcaagt gacttaggac aaacgacaga gcaaagtgaa tggaaaccgg
     6961 taatccacga cacaatcagt gacagtttag tggtacctaa tggcacaatg ggtcaacgtt
     7021 gggaagaagg taagaaatgg aacttaaaac tagaaacaga agatggttct aaaattaacc
     7081 ctacattatc aatgacagaa ggtgggtatg aattagaaac aattcaattc ccatactttg
     7141 atagtgatgg agatgggata ttcaatcgcc caattccaac tcgacaagtc actttagcaa
     7201 atggtgacaa agtccgtatt gctacaattt ttgacttaat ggcaagtcaa tatggcgtgc
     7261 gtcgttttga tcataaattg gaatcaaatg gatacgacga tgcagaatca aaatatacac
     7321 ctgcttggca agaagccatt tcaggcgtaa aacaaagtgt tgtcattcaa gtagctaaag
     7381 aatttgcgca aaacgctatc gatactgaag ggcgttcaat gattatcatg ggtgcgggta
     7441 ttaaccattg gtttaactca gatacgattt atcgttcaat cttaaactta gttatgttat
     7501 gtggctgtca aggtgtgaat ggtggcggtt gggctcacta tgtgggacaa gaaaaatgtc
     7561 gcccgattga aggatggagt actgtcgcat ttgcgaaaga ctggcaagga ccaccacgtt
     7621 tgcaaaacgg aacaagttgg ttctattttg cgacagacca atggaaatat gaagagtcaa
     7681 atgtagatcg tttaaaatct ccattagcta aaacagagga gttaaagcat caacatccag
     7741 ctgattataa tgtgttagca gctagacttg gttggttacc atcatatcca caatttaata
     7801 aaaatagctt gttgtttgca gaagaagcta aagatgaagg tatagattct aatgaagcga
     7861 ttttgcaacg tgcaattgat gaagtgaaat caaaacaaac acaatttgca attgaagatc
     7921 ctgatttgaa aaagaatcat ccaaaatcat tatttatatg gcgttcaaac ttaatttcaa
     7981 gttctgcaaa aggtcaagaa tactttatga agcatttact tggcacaaaa tcaggattat
     8041 tagctacacc aaatgaagat gaaaagccag aagaaattac gtggcgtgaa gaaacaacag
     8101 gtaagttaga tttagtggtc tctttagact tcagaatgac ggcaacaccg ttatattctg
     8161 acattgtttt gccagcagcg acttggtatg aaaaacatga tttatcatca acggatatgc
     8221 atccatatgt acatcctttc aatcctgcta ttgatccatt atgggaatca cgttcagact
     8281 gggatattta taaaacattg gcaaaagcat tttcagaaat ggcaaaagac tatttacctg
     8341 gaacgtttaa agatgttgtg acaacaccac ttagtcatga tacaaaacaa gaaatatcta
     8401 caccatatgg cgtagtgaaa gattggtcga agggtgaaat tgaagcggtg cctggacgta
     8461 caatgcctaa ctttgcaatt gtagaacgcg actacactaa aatttacgac aaatatgtca
     8521 cacttggtcc tgtacttgaa aaagggaaag ttggagcaca tggtgtaagt ttcggtgtca
     8581 gtgagcagta cgaagaacta aaaagtatgt taggcacgtg gagtgataca aatgatgatt
     8641 ctgtaagagc gaatcgtcca cgtattgata cagcacgtaa tgtcgctgat gccatactaa
     8701 atatttcatc agctacgaat ggaaagttat cacagaaatc atatgaggat cttgaagaac
     8761 aaactggtat gccgttgaaa gatatttcta gtgagcgtgc tgctgaaaaa atttcgttct
     8821 tgaatataac atcacaacca cgtgaagtta taccaacagc agtattcccg ggatctaata
     8881 aacaaggccg acgttactca ccatttacaa cgaatgtaga acgtttagta ccttttagaa
     8941 cgctaacagg tcgtcaaagt tattatgttg atcatgaagt tttccaacag tttggtgaaa
     9001 gcttaccagt atataaaccg acattgccgc caatggtatt tggtaataga gataagaaaa
     9061 ttaaaggtgg tacagatgct ttggtactgc gttatttaac gcctcatgga aaatggaata
     9121 tacactcaat gtatcaagat aataagcata tgttgacact atttagaggt ggtccaacgg
     9181 tttggatatc aaatgaagat gctgaaaaac acgatatcca agataatgat tggctagaag
     9241 tgtataaccg taatggtgtt gtaacggcaa gagcagttat ttcgcatcgt atgcctaaag
     9301 gtacaatgtt tatgtatcat gcacaagata aacatattca aacgcctggg tcagaaatta
     9361 cagatacacg tggtggttca cacaacgcgc cgactagaat ccatttgaaa ccaacacaac
     9421 tagtcggagg atacgcacaa attagttatc actttaatta ttatggacca attgggaacc
     9481 aaagggattt atatgtagca gttagaaaga tgaaggaggt taattggctt gaagattaaa
     9541 gcgcaagttg cgatggtatt aaatttagat aaatgcatag gatgccatac gtgtagtgtg
     9601 acatgtaaaa acacttggac aaatcgtcca ggtgctgagt acatgtggtt caacaacgta
     9661 gagactaagc cgggcgtagg gtatccgaaa cgttgggaag atcaagaaca ttacaaaggt
     9721 ggttgggtac taaatcgtaa agggaaactt gaattaaaat ctggaagtag aatttcaaaa
     9781 attgcattag ggaaaatttt ctataatcca gatatgccac taattaaaga ttattacgag
     9841 ccatggaact ataattatga acatttaaca actgcgaaat caggtaatca ttctccagtt
     9901 gctagagcat attcagaaat tacaggggat aatattgaaa ttgaatgggg acctaactgg
     9961 gaagatgact tggctggtgg tcacgttaca ggtccaaaag atcctaacat ccaaaaaata
    10021 gaagaagata ttaaattcca atttgacgaa acttttatga tgtatttacc acgtttatgt
    10081 gaacattgct tgaatccaag ttgcgttgcc tcatgccctt caggtgctat gtataaacgt
    10141 gatgaagatg gcattgtatt agttgaccaa gacgcatgtc gtggatggcg ttactgtatg
    10201 acaggttgtc catataagaa agtttacttc aattggaaaa cgaacaaagc tgaaaagtgt
    10261 acattctgtt tcccgagaat cgaggcgggt atgcctacgg tatgctctga aacttgtaca
    10321 ggacgtatgc gatacttagg tgttttatta tatgatgcag atcgagttca tgaagcggct
    10381 tcagcagtag acgagaaaga tttatacgaa aaacaattag atatattttt aaatccattc
    10441 gacgaagaag tcattgcaca agctgaaaaa gatggcatta gttatgattg gattgaagct
    10501 gcacaaaact caccaattta taagttagcg attgaatata aactagcatt tccattacat
    10561 cctgaattta gaacgatgcc gatggtttgg tattgcccac cacttagccc aattatgagc
    10621 tatttcgaag gtaaaaatac aaatcaaaat ccggatgcga ttttcccagc cattgaagaa
    10681 atgcgcttgc cgattgaata tttagcgaat attttcacgg caggagatac aaaaccagta
    10741 aaagaagcat tgcagcgtat ggcaatgatg agaagttaca tgcgttccca agttacacaa
    10801 caaccatttg atacatcacg tttagagcga ttaggtttaa ctgaacgcca aactaaagat
    10861 atgtatcgct tattaggact tgcaaaatat gaggatcgat tcgttattcc aacgtcacat
    10921 aaggaaacat atttagatac gtatcatgct caaggtagta cgggatataa ctatggtggc
    10981 gaacactttg gtgataactg tgaaggttgt ggtgttgcag taggttcagg aaaaactggt
    11041 caagaaattt ataatgagaa tttctatgga gggattttcc gtgattaatt tcgataattt
    11101 taaaaagtat caagaaagtt ttggatatat agcgcaacag ctatgctttc cggaaaaatt
    11161 aacatttcat ccaaagactt ttgaagaaac aatttcgaag gatcatccag ggtatgacga
    11221 tttaatagca tttagaaatg taatgatgac attttcatta tcagaaatca aagcgattta
    11281 tactgataca tttgatttta caaaaaaagc gccattatat atgacgtata acaagtttga
    11341 tacacaaaaa gaacgtgggc aaatgttagc gaaactaaag gtattgtacg agatgtttgg
    11401 actagaaatg gtagacaatg aactttctga ttatctaccg ttgatgttgc aatttttaca
    11461 agtggcagaa tggcgaaatg atccaagagc tgaagagaat attcaattaa tcattatgat
    11521 tattgaggat ggaacatatg tgatggcgaa tgcacttggt gaagataaca atccatatgc
    11581 ctatgtgatt caagcactta gaaaaacatt gaaattatgt ttgacatcac caaaaggagt
    11641 gaaacatcat gcttaatcag tttttatggg ttatatttcc gtatttatgt ttagttgtct
    11701 ttgtcgcagg tcatattgca cgttatcgtt atgataaatt ttcgtggaca gcaaaatcta
    11761 gtgaattaat tgaaagaaaa agattgatgt ggggtagttt gctatttcac ttaggtatca
    11821 ttccggtatt ttttggacat gttgtaggat tattaatacc taagtcgtgg atggatgctg
    11881 ccggtgtgtc tgagcattta tatcatattg gtgctgttta tatagggagt atctttggta
    11941 taattacatt ggtagggatg tttttattaa cagcgagacg tgttactacg aaaagtattc
    12001 gcagattaag ttcagcgtcc gatatctttg ttaatttctt attattactt attgtattta
    12061 tgggatgtta tgcaacatta gtaacaaacg tacagcatcc agattttaac tatagaactt
    12121 cattatcaat ttggtttaga caattattta tgttgaaacc agatgcatca ttaatgagtg
    12181 gtgtaccaat tgcatttaaa attcatatat tattaggttt cactattatg gcttgttggc
    12241 cattcacacg tttagtacat gtttggagtg tacctttgac atatataaat cgcagatata
    12301 ttatttaccg taagaataaa gtgtaagtat aagaggtgaa aggatttgac accagaagca
    12361 attattgaag atcgtagatt tcaagagaca ttagataaaa tacgcaaaga agaaggttat
    12421 gattttgcgg caatcgcttt ctatgaatct aacaaaccat catctccaat aaaatggcac
    12481 tatgtttcag gtaataaaaa taatcggttt aaattaatta ttttaagaaa aggccgtgga
    12541 ttagctggaa ctgttatgaa aacagggaag cgtatgatta tagctaatgt cggtttagca
    12601 ttgggaccag aagaaaagat tgactatcca attttattaa gcgaatcatt aacagcagtc
    12661 ttagctgtac cgctttggta caaaaatcaa gtgtatggtg tactattatt tggtcaaaga
    12721 gatggacgtt ctttaccaaa aatatttgat aacgatgata ttcaacgcaa atttggaatt
    12781 ttcaatgatg ataaataatg agatgaatta gggtgtaggt catgattaat gaggacagta
    12841 tacaattaga agctttatta aaaaaatact atgaacattc aatcgaaaaa atagtatttg
    12901 ccgacgatag tggtaaaatt attgcgatga atgatgcagc taaagatata ttatccgaag
    12961 aagataatta tagtgcagta gcgaatgcga tttgtcacag atgtgaaggg tatacgaatg
    13021 cctatgacgt acaatcatgt aaagattgtt tcttagagtc catgcaagta caagctacga
    13081 attttcaagt gtttatgaag acgaaagatc aaaaagtgat gccgtttact gcaacttatc
    13141 agcttattga tcaggataga gggattcatg catttacgct tcaaaatgtc tcatcacaaa
    13201 ttgaacaaca agaaaaattg catcaacaac gtatgatgcg taaaacgatt tccgcacaag
    13261 aaaatgagcg aaaacgaatt tctagggaat tacatgatag tgtgattcaa gaaatgttaa
    13321 atgtagatgt tcaattgcga ttgttgaaat atcaagaaga tacagcaaag cttttggaag
    13381 acgcagaaaa cattgaatac attgtcgcta aattaatcga tgatatacgt aatatgtcgg
    13441 tggaattaag accagcctct ttggatgatt taggccttga ggcagcgttt aaatcatatt
    13501 ttaaacaatt tgaagaaaat tatggggtta aaatcatata tacttccaat attaaaaata
    13561 cccgttttga tagtgatatt gaaacggttg tttaccgagt agtacaagaa gctattttaa
    13621 atgcgttgaa atacgctgat gtaaatgaaa taaatgtagg tatccgtcaa actgggcgcc
    13681 atttggttgc tgaagtaata gacgcgggaa atggttttga tccaagttct aagcctaaag
    13741 gttctggact cggtttatat ggaatgaatg aacgagctga attagtcagt ggtagcgtca
    13801 acatcgaaac gaaaattggt gagggcacca atgttacact gaacattcca atttgaaaca
    13861 tacattgggg gaataaaatt gaaaatagtc attgccgatg atcacgctgt tgtccgcacg
    13921 ggattctcta tgattttaaa ttatcaaaat gatatggaag ttgttgcaac agctgcagat
    13981 ggtgtcgaag cttatcaaaa agtaatggaa tataaacctg atgtgttact aatggattta
    14041 agcatgccac caggtgagtc aggtcttatc gctacgagta aaattgctga cagttttcca
    14101 gaaactaaaa ttttaatatt aaccatgttt gatgatgaag agtacttgtt ccatgtgttg
    14161 cgtaatggtg cgaaaggtta catattgaaa aatgcacctg atgaacaatt attgttagct
    14221 attcgaactg tatataaagg tgaaacatat gtagatatga aacttacaac atctttagtg
    14281 aatgaatttg tatctaattc aaatcaggac actgcaaaca catcagatcc ttttaaaatc
    14341 ttatcaaaac gagaactaga aatattgcca cttattgcca aaggttacgg gaataaagaa
    14401 attgcagaga agttatttgt ttctgtaaag acagtagagg cacataaaac gcatattatg
    14461 acaaagcttg gcttgaaaag taaaccagag ctcgttgaat atgcattgaa aaagaaatta
    14521 ttagagtttt agagtttgaa attaatacca tacaaagcgc cagaggaaat gattatcttc
    14581 tggcgctttt gtatgtttcg aaatatattt ttttataacc gtatggaagc taatactttc
    14641 tccttttgga aagccatact tattttattg gaaacggaat aacttttaat atagttattg
    14701 atatagagaa taaatgagta gttgtctatt ttggacatga catgttagtt agggtgtatt
    14761 cagtgtttta gtctctaagt gaggattaaa aattgtcttt cttattctca ttgctcaaaa
    14821 aataattaac agcttctgaa atatatgatg ctaaattttg attatcatat gaattaaagt
    14881 aatctttgaa acgcgtatca ttttcatatg ttttagcaat acagcataat gtttcatcat
    14941 caaaatcagc gattgtgttc ataaaagatt tccattcttc cacgatatca ttacagtctt
    15001 ggattggata atgacttaaa gacatttgat tgaattgatt gaagatcatt gtcaatttct
    15061 gtctgatatc ctgtgattgt aagttgtctt gacggttaat aaatgattga tatgccttcg
    15121 tatgaccata ctttatagat gcttctttat catattgtgt ctgtagtgac ttgtttaaaa
    15181 tgctggtatc tattagagag tgtcctttaa taaattcaca tacagagcgt ctaattaatt
    15241 ataagtcaga aatcttttta tctaacttgc ttacttgctc tgataataac tttcgcaatt
    15301 gttcattatc atatgaaatg tattgtttta ttttagcgat atcaaaatca aaagacttga
    15361 gtattaaaat tttttgtaat ttttctaagt cttgctgttt ataaacgcga taatttttat
    15421 catttttatt tggaactaat aatcctattt catcgtaata atgtaaagtt cgtttagtta
    15481 cacctgtaat ttcaataata tcttttaaag tgtatgtgta tgccaaaaat cattcacctc
    15541 atttattatt ttttgtgctg gaagtgttgt ggcaacttca ataggtatgt ttgttttttg
    15601 taaaaagtat ttcaccaagt gatagccaca tgcatacccg gcagcatgag ataagccaac
    15661 gatagaccta ccttgaagtt tattaatatc atcaccatag agaaaaggca tcgattcaaa
    15721 tatatgcttt aaatgtaaat gattataaat agtattttta atttttacgt tatcacgact
    15781 ccaattagta tttgttaccc acggtccaat atgcgcttta ccatacaatg attctacata
    15841 gttttcagct aatccttctg cgataatcaa ttcaatcaaa cttccgccat cccaatcaat
    15901 atattgatag cgcatattat gatttacttc atgtgcaatt aagcttttca tagaattaat
    15961 tgtacttgta ctcggcacta aataaatcat aatataacct ggtatgccac catcaccacc
    16021 gcgattttta tttagataca taagtggttt ttggctatct ccaagtaata ctgtgaaatg
    16081 atagttagaa acttgagatg agatactata atttgcaaat tgatctatag caacttttaa
    16141 atatttttcg caattactcc agaaatcatt attcaggtat tgtattgaca tttggtcaga
    16201 catgcgtaaa tctttgggag acttttgaaa ggcatctaaa aattgaattg cactcattgt
    16261 ctgcttggca tcattatgaa taggaatctg ttgcacctca aatttctttt taaaaggtac
    16321 taatatttct ttagtgaaaa ttgaatcacg gtcgtttaat ggtgtcttga gtatattctt
    16381 atagacggaa tctgatctaa taatattgat tttatacatg ataaacctcc ttacgttgtc
    16441 agcataaagg gtaacgtaac gtgattttca agcagtaatt gtaactaatt gaaaaaaatt
    16501 taagaaatgc atgtgagtga tccttaataa tctgattaaa atgatgacaa aatagtgtct
    16561 aaatgcatcg taaagggaca ttgtataggg gaaattacac cataaataag ggaaaatcct
    16621 tatgttcact ttttcacaat cataataaaa taaatgtgta gtcaatactt tgtctatatt
    16681 ggggagtggg acagaaatga tattttcgaa aaatttattt cgtcgtcccg ccccggcaag
    16741 gttgactaga
//
LOCUS       DAMZPP010000059        16595 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMZPP010000059 DAMZPP010000000
VERSION     DAMZPP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16595
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.059"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..1038
                     /locus_tag="POH73_001414"
     CDS             <1..1038
                     /locus_tag="POH73_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6887374.1"
                     /translation="HLTTKGSLSRDYAVNKDNKAFYFLLRDDDYFAKVVNGQARNTGE
                     RVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDIDQLRKEKDKDDNSIY
                     DKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYLTHSSAGILSKKFVN
                     QVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATFQRNPGFNETEKGSY
                     GPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKSDKNLSIIRKNGRKS
                     VFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIASPITPLVDTGNEQRQ
                     DLEKVEKAINQ"
     gene            1076..3082
                     /gene="ggt"
                     /locus_tag="POH73_001415"
     CDS             1076..3082
                     /gene="ggt"
                     /locus_tag="POH73_001415"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDI6887375.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVVEPFASGIGGGGYLLYYEQSTGSITAFDARETAPAHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHSANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLANYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLQSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSKL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVTGGINEIAPYKRPLSNMAPTIVMHHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIARGHAME
                     HKPDAYIGDVHGLHVDLNTRDASGGADDTREGTVMGGEVLSIRKQPLPYRQMYGSNVY
                     RVYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFSEVSAHIEDLRSYENAGENYIDIT
                     WLARKKGYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(3619..4245)
                     /locus_tag="POH73_001416"
     CDS             complement(3619..4245)
                     /locus_tag="POH73_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDI6887376.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPEDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVLVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            4454..5032
                     /locus_tag="POH73_001417"
     CDS             4454..5032
                     /locus_tag="POH73_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDI6887377.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKLEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGKQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            5415..6512
                     /gene="ugpC"
                     /locus_tag="POH73_001418"
     CDS             5415..6512
                     /gene="ugpC"
                     /locus_tag="POH73_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDI6887378.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            6525..7796
                     /locus_tag="POH73_001419"
     CDS             6525..7796
                     /locus_tag="POH73_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDI6887379.1"
                     /translation="MSKILKYITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            7799..9067
                     /locus_tag="POH73_001420"
     CDS             7799..9067
                     /locus_tag="POH73_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI6887380.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYVINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     SKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            9069..9908
                     /locus_tag="POH73_001421"
     CDS             9069..9908
                     /locus_tag="POH73_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI6887381.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            10085..11161
                     /locus_tag="POH73_001422"
     CDS             10085..11161
                     /locus_tag="POH73_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI6887382.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAACA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRPDSQFLYKSAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHDAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            11186..12226
                     /locus_tag="POH73_001423"
     CDS             11186..12226
                     /locus_tag="POH73_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI6887383.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNCKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            12281..13249
                     /locus_tag="POH73_001424"
     CDS             12281..13249
                     /locus_tag="POH73_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDI6887384.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVAESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPTEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(13608..14102)
                     /locus_tag="POH73_001425"
     CDS             complement(13608..14102)
                     /locus_tag="POH73_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HDI6887385.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            14335..15714
                     /gene="uhpT"
                     /locus_tag="POH73_001426"
     CDS             14335..15714
                     /gene="uhpT"
                     /locus_tag="POH73_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDI6887386.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            complement(16073..>16595)
                     /locus_tag="POH73_001427"
     CDS             complement(16073..>16595)
                     /locus_tag="POH73_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6887387.1"
                     /translation="LSSYDDFEYMKAGIQHHVLDYLLKPVDHAQLEVILGRLVRTLLE
                     QQSQNGRSLAPCHDAFQPLLKVEYDDYYVNQIVDQIKQSYQTKVTVSDLIQHIDVSES
                     YAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHYEIADKVGFSEYKMFSYHFKKY
                     LQMSPSDYCKQAK"
BASE COUNT         5583 a         2507 c         3274 g         5231 t
ORIGIN      
        1 cacttaacaa cgaaaggttc gttaagtcgt gattatgctg tgaataagga caataaagcg
       61 ttttatttct tattaagaga tgatgattat tttgcgaaag tggttaatgg acaagcacgt
      121 aatactggag agcgtgtatc ggctgaagat gttaagtttt ctttagatag agcacgtgat
      181 aaaaagtctg tgcctaacaa taatacttac aatatgcaca aacatataaa tgacatcaag
      241 atattaaaag atgaggacat cgatcagttg cgtaaagaga aggacaagga cgataattcc
      301 atctatgata agttgattaa agcgtataac gtcaaatcgt taacgacaga tggtcaaaaa
      361 gtaaataata aagacggtat ttatcaaatt gttaaaatta cgacagatca atcgatgcct
      421 cgagaggtaa attacttaac acactcttcg gcaggcattt tatctaaaaa atttgttaat
      481 caagtaaatc aagaatatcc aaaaggatat ggggatagca gtaccattcc tgcaaattca
      541 gatggaaaaa atgcgcttta tgcaagtggc gcatacatta tgacacagaa aaatgcatat
      601 caagcaacgt ttcaacgtaa tccaggattc aacgaaacag aaaaaggtag ttatggacca
      661 gctaaaatta aaaatattac attgaagttt aatggtgacc cgaataatgc attgtcagaa
      721 cttagaaatc attcaattga tatgttggca gatgtgaatc aaaaacattt tgatttaatt
      781 aagtcggata aaaatttaag cattattcgc aaaaatggac gtaagtcagt ctttttaatg
      841 ctaaatatta aaaaaggtat atttaagacg catccaaact tgagacaagc agtagttaat
      901 gcgatagatc aggatcaatt tattaagttt tatcgtggcg ataaatttaa aattgcatca
      961 ccgattacac cacttgtcga tactggtaac gagcaacgtc aagatttaga aaaagtagaa
     1021 aaagccatta atcaataatg ttttaaatat ttaacagaaa gtaggaggat atagtatggt
     1081 cattaactta aatgacaaac agacaaaaac atctaaagaa gggttaattt ccgtatcaca
     1141 tcctcttgcg gctaaaattg gtaaggatgt attagatcaa ggtggcaacg ctatggatgc
     1201 cgtgattgca attcaactgg cattgaatgt ggtagaacca tttgcatcag gtattggtgg
     1261 tggcgggtac ttgctatatt atgagcaaag tactggcagt ataactgcgt ttgatgcgcg
     1321 tgagacagcc cctgcacatg tagacaaaca attttatcta gatgattcag gcgaatataa
     1381 atcatttttt gatatgacta cacatggtaa aactgtcgct gtgccagcaa ttccaaagct
     1441 gtttgattat attcacaagc gttatgctaa attgtcattg gaagatttaa ttaatcctgc
     1501 aattgaacta gccattgaag gtcattcagc taattgggct actgaaaaat attcgcgcca
     1561 gcaacacgcg cgattgacaa agtatcacga aacggcacaa gtatttaccc atgaaaatca
     1621 atattggcgt gaaggtgatt ggattgtaca acccgaatta ggtaagacat ttcaaatatt
     1681 aagagaacaa gggtttaatg cattttataa aggtgacatt gcgaaacaat tagtcaatgt
     1741 tgtcaaagca tgtggtggga caatcacttt agaggatcta gccaattatg atattcagat
     1801 taaagcgcca atcagtgcga catttaaaga ctatgacatt tattcaatgg gaccatctag
     1861 ttctggcggt atcacggtaa ttcaaatatt gaagttatta gaacatgtcg atttacaatc
     1921 tatgggtcca agatctgttg attacttgca tcatttgata caggcgatgc atttagcata
     1981 tagtgatcgc gcgcaatact tggcggatga taattttcat gaggtgcctg tacagtcatt
     2041 aattgatgac gattatttaa aagcacgcag taagctcatt gatagcaata aagcaaatat
     2101 tgatatagag catggtgttg tgtctgattg cattagtcat acagatgttg aagaaaatca
     2161 taccgaaaca actcattttt gtgtgattga taaggaaggt aatattgctt catttacgac
     2221 atcaatcggt atgatttacg gatcgggtat cacgattcca ggctacggcg tgttattgaa
     2281 taccacaatg gatggttttg atgttgtgac gggtgggatc aatgaaattg caccttataa
     2341 acgaccacta agtaacatgg ctccaacgat tgtgatgcat cacgggaagc caatattaac
     2401 agtaggtgca cctggtgcta taagtatcat tgctagtgtt gcgcaaacat taatcaatgt
     2461 attagtgttt ggcatggata ttcagcaggc tatagatgaa cctagaattt atagtagcca
     2521 tcctaatcgc attgaatggg agcctcaatt ttcacaatct acaattttag cattgattgc
     2581 acgtggacat gcaatggaac ataaaccaga tgcctatatt ggagatgtac atgggctaca
     2641 tgttgacttg aatacacgtg acgcatcggg aggtgctgat gatacgaggg aaggtacagt
     2701 gatgggtggt gaggtattat caattagaaa acaaccatta ccatatcgtc aaatgtatgg
     2761 tagtaatgta tatcgtgtat atttcaatga tgtgcagtta cctttattgg cagatcaagt
     2821 gcgatggatg catgacaaat attgggttga tgaaagtgtc gttagaatca tcttctctga
     2881 agttagtgca catattgaag atttaagaag ttatgaaaac gcaggagaaa attatataga
     2941 tattacctgg ttagcacgaa agaaaggtta tcaagtaaca ttgaaggatg acggtttata
     3001 cttaactgat gatacatata cttcagtgaa acggaacaca aatgcatact atagatatga
     3061 tcgagatagt atcacaagat agtttatacg cttgatatga agtttgtagt ggataaggac
     3121 ttttgttgaa agaaaggatt cgatttcgaa ctttagcgtg acgcttgagt gtagtaatgt
     3181 tggaaatgct atacgaaacg ctagtcacct ttgtaaatat acaacacctc acatattcgt
     3241 ttttgatgta catcacaata gttagtctaa aatctaacgt gatgtggttt ggtacagttg
     3301 aacgagttat gtgaggtgtt tttataatgt gggattttaa tagatgattt ggatgtttct
     3361 ataattacag cataagtatt aatgttcgct actaagttta accagaagtt tttatgtgtc
     3421 ttcatacttt catatctact ctcaaaatat atcatcaaca ctatcatatg ttttaaaaat
     3481 atttacatca taactaaagt gaatgtcaaa atttaaattt gtaaaaacaa tagatgtata
     3541 taaaaagttg aaaaggattg taagaaaatc aaaaaaacac tgtatgacgt ttgattagtc
     3601 acacagtgta gataacaatt aaaatgttgt tgccaatttt tcagcattat taatactagt
     3661 tgctttaatt tcttcagttt tatgcggttc ggcattgtgt ccttcaataa tgattgtttc
     3721 atatgatggc acacctaaga atgtcataat tgttcttaaa taacggtcac ccatttcaaa
     3781 atcagcagca ggtccttcag tataatatcc accacgtgat tgaatgtgta atactttttt
     3841 gtcagttagt aaaccttgtg gtccttcagc agaatattta aaagttttac ctgcaattga
     3901 aatagcatca atatatgctt taactacagg tgggaaagaa aggttccaca taggcgttac
     3961 aagcacatat ttatctgcac ttaaaaattc ttctaaaatg tcactcaatc ttgaaacttt
     4021 catttgttca tcatcagtta acgtttcgcc attactcatt ttcccccaac cagttaatac
     4081 atctttgtca ataactggaa tataagtttc aaataaatca atatgtttca cttcatcttc
     4141 aggatgttgt tgttgatatg tttcgataaa tgctttacca gccgccatag aatttgatac
     4201 cagttcatta aaagggtgtg ctgtaatata taatactttt gccatttgaa aattctcctc
     4261 tgtttctgtt attttcttaa gtataattat tatactaggt ataaaattta atatcaatca
     4321 aaatattcaa attaccatca ttttcttcat ctatattttg cagtactact aaagtatgag
     4381 tgcatttaat tatgaaatag ttgatttaga atatatactt aatacccaaa atatatgaag
     4441 gatggatgcc actatgacaa agcgaccaaa acgtattttg gcaacaatta tcatttttct
     4501 ttcactatta tttacgatta tttatataga tgacatacaa aaatggttta accaatatac
     4561 cgataaattg acacaaaatc ataaaggaca aggacactca aaattggaag acttttttag
     4621 agggagtcgg attactgaga cttttggtaa atatcaacat tcaccatttg atggtaagca
     4681 ttatggtatt gattttgcat tgccaaaagg tacaccaatt aaagcgccga cgaatggtaa
     4741 agtaacacgt atctttaata atgaattagg cggcaaggta ttacagattg ccgaagataa
     4801 tggagaatat caccagtggt atctacactt agacaaatat aatgtcaaag taggtgatcg
     4861 agtcaaagca ggtgatatta ttgcatattc aggcaataca ggtaaacaaa cgacaggcgc
     4921 acatttacat tttcaaagaa tgaagggtgg cgtaggtaat gcatatgcag aagatcctaa
     4981 accgtttatc gatcagttac ctgatgggga acgtagccta tatgatttgt agttatagaa
     5041 gagtgcccgc agtctaaaaa atcaagcaat cattgtgtga gtataatact tacataatgg
     5101 ttgctttttt caatgaaaat cgaaatgcta agtcatactt gtttgattta gatattattt
     5161 aaaatgtaag acaaggttgt tagcattggc agtgaaattt cgcacgtaaa aaatcattat
     5221 tgacacacta aaaatagttg tgtgctatat caattttctg tataaaagtt taattctgac
     5281 agtaatgtaa acgtttacaa tttatgattg acattaataa taagtgacta tatgatttat
     5341 gtaagtattt gtgcaaacgt tttcacaaag tgtattgcac aatcaaactg taaacaaagt
     5401 atgggaggca taacatggca gaactaaagt tagagcatat taaaaagacg tatgataaca
     5461 acaatactgt agtgaaagat tttaatctac atattactga caaagaattc attgtatttg
     5521 ttggaccatc gggatgtggt aaatcaacaa cattacgaat ggttgctgga ctagagtcta
     5581 tcacatctgg agatttttat attgatgggg aacgcatgaa cgatgttgaa ccaaagaata
     5641 gagatattgc gatggtattt caaaactatg cattatatcc acatatgact gtttttgaaa
     5701 atatggcatt tgggctaaag ctacgtaaag tgaataaaaa agagattgaa caaaaagtta
     5761 atgaagcagc tgaaatatta ggattaactg agtatcttgg tcgtaaacca aaagcgttat
     5821 ctggtggaca gcgtcaacgt gttgctttgg gcagagctat tgttagggat gcgaaagtct
     5881 ttttaatgga tgaaccatta tcgaatcttg atgcgaagct tcgagtacaa atgcgcacag
     5941 aaatattgaa attacataag cgacttaata ctacgacaat ttatgttacg catgatcaaa
     6001 ctgaagcatt gacgatggct agtcgaattg ttgttttgaa agatggcgac attatgcaag
     6061 tcggcacacc tagagaaata tatgatgccc ctaattgcat ttttgtggcg caatttatcg
     6121 gctcaccagc aatgaatatg ttgaatgcca cagttgaaat ggatggattg aaggtaggaa
     6181 cacaccattt taaattacat aataaaaaat ttgaaaagtt aaaagctgct ggctacttag
     6241 ataaggaaat tattttaggt attcgagctg aagacattca tgaagaacca atatttattc
     6301 aaacctctcc agagacacaa tttgaatctg aagtagttgt atccgaactg ttaggttcag
     6361 aaattatggt acatagcaca ttccaaggaa tggaattgat ttctaaatta gattcaagaa
     6421 cacaagtgat ggcgaacgac aagattacac tagcatttga tatgaataag tgtcactttt
     6481 ttgatgaaaa aacaggaaat cgtatcgtct aagggggagt attcatgtct aaaattttaa
     6541 aatatatcac gttagccgtg gtaatgttat taatcgtaac tgcatgtggc cctaatcgtt
     6601 cgaaagaaga tattgataaa gcattgaata aagataattc taaagacaag cctaaccaac
     6661 ttacgatgtg ggtggatggc gacaagcaaa tggcgtttta taaaaaaatt acggatcaat
     6721 atactaaaaa aactggcata aaagtaaagc ttgtaaatat tggtcaaaat gatcaactag
     6781 aaaatatttc tctagacgct cctgctggaa aaggtccaga tatctttttc ttagcacatg
     6841 ataatactgg aagtgcctat ctacaaggct tagctgctga aatcaaatta tcaaaagatg
     6901 agttgaaagg cttcaataag caagcactta aagcgatgaa ttatgacaat aagcaattag
     6961 cattgccagc tatcgttgaa acaaccgcac ttttttataa taaaaaatta gtgaaaaatg
     7021 caccgcaaac gttagaagaa gttgaagcta atgctgccaa actaactgat agtaaaaaga
     7081 aacaatacgg tatgctattt gatgctaaaa atttctattt taattatccg tttttattcg
     7141 gcaatgatga ttatattttc aagaaaaatg gcagtgaata tgatattcat cagctaggac
     7201 taaattcaaa acatgtcgtc aagaatgctg aacgattaca aaaatggtac gacaaagggt
     7261 atcttcctaa ggcagcaaca catgatgtca tgattggtct ttttaaagaa ggaaaagtag
     7321 gacaatttgt cactggacca tggaacatta atgaatatca agaaacgttt ggtaaagatt
     7381 taggagtaac aacattgcct acagatggtg gcaaacctat gaaaccattt ctcggtgtac
     7441 gtggttggta tttatctgaa tatagtaaac ataagtattg ggctaaagat ttaatgttgt
     7501 atatcactag taaagataca ttacaaaaat atacagatga aatgagcgaa attactggac
     7561 gtgttgacgt aaaatcatct aatccaaatt taaaagtgtt tgaaaagcaa gcacgtcatg
     7621 ctgaaccgat gcctaatatt cctgaaatgc gacaagtttg ggaaccgatg ggcaatgcaa
     7681 gcatatttat ctcaaatggt aagaatccta aacaagcgtt agatgaggcg acgaatgata
     7741 taacgcaaaa tattaagatt cttcatccgt cacaaaatga taagaaagga gattagttat
     7801 gacgaaacgt aaccctaaat tagcggcatt attatctgtt atacctggtt tgggacagtt
     7861 ttataataaa agacccatta aagggacgat attttttatc tttttcatca gttttatttc
     7921 tgtattttat agctttttaa atattggttt ttggggattg ttcacattag ggacagtacc
     7981 taagttagac gattctcgtg tcttacttgc acaaggtatt atttctatct tactcgttgc
     8041 ttttgcaatc atgctatatg tcattaatat tttagatgca tatcgtaatg ctgaacgatt
     8101 taatcgcaat gaggaaataa aggatccgaa ggcacgtatg gttgcaacat gggacaagac
     8161 gttcccatac ttactaatct caccaggtac attcttattg atatttgtag ttgtatttcc
     8221 attaatattt atgtttggag tagcatttac aaactacaat ttatacaacg cgcctccgag
     8281 acacacatta gaatgggttg gtttagataa ctttaaaacg ttattcacaa ttggcgtttg
     8341 gcgtaaaaca tttttcagtg ttattacttg gacattagta tggacgcttg ttgcaacgac
     8401 acttcaaatt gcattagggc tgtttttggc aattattgta aatcaccctg tcgtcaaagg
     8461 taagaaattt atccgcactg tgttaatcct accttgggct gtaccatcat ttgtgacaat
     8521 tttaatattt gtagcgttat ttaatgatga atttggtgcg ataaataatg atatattgca
     8581 acctttatta ggtgttgcac cagcatggtt aagtgatcca ttttggtcaa aagtggcatt
     8641 aattggtatt caagtatggc ttggtttccc attcgtcttt gcactgttca ctggtgtact
     8701 gcaaagtatt tcatcagatt ggtacgaagc agcagatatg gatggtgcgt ctagttggca
     8761 aaagttcaga aacatcacat tcccgcatgt catttacgcc acagcgccat tgttaattat
     8821 gcaatatgca ggtaatttca ataattttaa tcttatttat ctatttaata aaggtggtcc
     8881 accagtgtca gggcagaatg ctggtagtac agatatcttg atatcttggg tgtataatct
     8941 gacatttgag tttaacaact ttaacatggg tgcagttgtg tcattaatta ttggatttat
     9001 tgttgctatt gtcgcattta ttcaattcag acgtacaagt acgtttaaag atgagggagg
     9061 tttataagat gacaaagaag aaaaacatat taaaagcaat cggtatttac agttttatag
     9121 cgatgatgtt tgtcatcatt ttatatccac tactgtggac atttggcatt tcccttaatc
     9181 caggtacgaa cttgtatggt gccaaaatga taccagacaa tgcaacattt aaaaattatg
     9241 cattcttact attcgatgac agtagtcaat acctgacttg gtataaaaat acgcttatcg
     9301 tagcatctgc aaatgcactg tttagtgtga tatttgtcac gttaacagca tatgcttttt
     9361 ctagatatcg ctttgttggt cgtaaatacg ggctgattac atttttgatt ttacaaatgt
     9421 tccctgtatt aatggcaatg gtcgcaatct atattttgct aaatacaatt ggattattag
     9481 attctttatt tggactaaca cttgtatata ttggtgggtc tataccgatg aatgcctttt
     9541 tagtgaaagg ttacttcgat acgattccaa aagaacttga tgaatctgcc aaaattgatg
     9601 gtgctggcca tatgcgtatt ttcttacaaa ttatgcttcc attagctaag ccaattttag
     9661 cagttgttgc tttgttcaat tttatggggc catttatgga ctttatatta cctaaaatac
     9721 tattaagaag tcctgaaaaa ttcacattag cagttggatt gttcaacttt attaatgata
     9781 agtatgcaaa taatttcaca gtgtttgcag caggggcaat tatgattgca gtacctatag
     9841 caatcgtatt cttgttcttg caacgctact tagtatcagg tttaacaaca ggtgcgacaa
     9901 aaggttagtt tgaaattggg agcggggcag aaatgataaa gaaccactaa tgaatttatt
     9961 gtgtagtggc tcttacaaat tagccacagc taatatgtat ctaaaaatag cctagaacgt
    10021 tgattgatgt ttaaggctcg acatacgtac taatgacgat aaagatttaa aggaggacgt
    10081 tatgatgacg attaaagttg gaatcattgg gtgtggtggt attgcgaatg gcaagcacat
    10141 gccaagttta caaaaagttg aaaatgttga aatgatcgca ttttgtgacg tagacatttc
    10201 gaaagcagcg tgtgcggcag aagcatacgg tactgacaat gcaaaggttt atgatgatta
    10261 caaagcattg ttaaaagatg acacgattga tgttatccat gtttgtacgc caaatgactc
    10321 gcattgtgaa attactgtag cagggttgca tgccggtaaa catgtgatgt gtgaaaaacc
    10381 aatggctaaa acgacagcag aagctcaaaa aatgatagat acagctaaat caacaggtaa
    10441 aaaattaaca ataggttatc aaaatcgctt cagaccagat agtcaatttt tatataaatc
    10501 agcgcaacgt ggcgacttag gtgacattta tttcggaaag gcacatgcta ttcgtcgtcg
    10561 agcagtacca acatggggtg tatttctaga cgaagaagct caaggtggag gaccattaat
    10621 cgatatcggt acacacgctt tagatttaac gttatggatg atggataatt atgaaccaga
    10681 atcagtgatg ggttcaacat tccataaatt aaataaacag catgatgcgg caaacgcttg
    10741 gggttcatgg aatccagatg aatttacagt tgaagattct gcgtttggct ttattaaaat
    10801 gaagaacgga gcgacgatca ttttagaatc cgcttgggcg attaattctt tagaagtgga
    10861 tgaggcaaaa tgttcattat caggaacgaa agcaggtgct gatatgaagg atggtctacg
    10921 tattcatggt gaagatatgg gcacacttta taccaaacac gttgaattgg aaaacaaagg
    10981 cgtcgacttt tatgaaggta atgaagtgga tgaagctgaa gaagaagcaa aagcttggat
    11041 tgatgcagtt gtaaatgata ctgaaccagt tgtgaaaccg gaacaagcaa tggtagttac
    11101 aaaaattctt gaagcgattt atcagtctgc aaaatcaggc aaagcaattt actttgaata
    11161 acatcatacg gtaaggaggc acatcatgac aaaattaaaa gttggtgtga taggtgttgg
    11221 tggtattgca caagaccgtc atattccagc attgctgaaa ctcaaagaca cagtctcatt
    11281 agttgcagta caagatatta atacagtgca gatgattgat gttgcgaagc gctttaatat
    11341 acctcatgca gttgagacac ctagcgagct gtttaaactt gttgatgcgg tggtcatttg
    11401 tacacctaat aaattccatg ctgatctttc tatagaggca ttgaaccatg gtgtccatgt
    11461 attgtgtgaa aagccaatgg cgatgacgac ggaagagtgt gaccgcatga ttgaagcggc
    11521 taataaaaat tgcaaattat taactgtcgc atatcattat cgtcacacag atgtggcaat
    11581 tactgctaaa aaagcaattg aatcaggtgt ggttggaaaa cctttagtag cacgtgtaca
    11641 agcgatgcgt aggcgtaaag tgcctgggtg gggtgttttt accaataagg cattgcaagg
    11701 tggcggtagt ttaatcgatt atggttgcca cttgttagac ttatctttgt ggctactagg
    11761 taaagatatg gtgccgcatg aagtgctagg aaaaacatat aatcaattga gcaaacaacc
    11821 gaatcaaatt aatgattggg gaacatttga tcacactaaa tttgatgtcg atgatcatgt
    11881 tactagttat atgacatttg ccaatcgagc aagcatgcag tttgaatgtt cgtggtctgc
    11941 aaatataaag gaagataagg tacacgttag tttatcaggc gaagatggcg gtatcaattt
    12001 atttccattt gaaatatatg agccccgctt tggaactatt tttgaaagca aagctaatgt
    12061 tgagcataac gaagacattg ctggtgagag acaggcgcgt aactttgtca atgcgtgttt
    12121 agggatagaa gagattgtgg taaaaccgga agaagcacgc aatgtaaatg cccttataga
    12181 agcgatttac cgtagcgatc ttgataacaa gagcatacaa ctttaatgat tattatatat
    12241 aatacaaaat tctcaatata aaaagaagga gtgcttttca atgaaaatag gtgtattttc
    12301 agtattattt tacgataaaa atttcgaaga tatgttagat tatgtcgcag aatctggatt
    12361 ggatatgatt gaagttggaa caggtggtaa cccaggagat aaattttgta agttagatga
    12421 gttgttagaa aatgaagaca agcgacaagc atttatgaag tcaatcacag acagaggctt
    12481 acaaataagt ggtttcagtt gtcataacaa tccaatttct ccagatccga cagaagcgaa
    12541 agaagcggat gaaacgttac gtaaaacaat ccgtttagca aatctattag acgtgccagt
    12601 tgttaataca ttttctggca ttgcaggatc agatgatacc gctaaaaagc ctaattggcc
    12661 tgttacacct tggccaacag cctactctga aatttatgat tatcagtgga atgaaaagtt
    12721 gataccatat tggcaagatt tagctgagtt tgcaaaagag caagatgtaa aaattgccat
    12781 agagttgcat gcaggatttt tagttcatac accatatacg atgttgaagt tacgtgaggc
    12841 tacaaatgaa tacatcggtg ctaacttaga tcctagtcat ttatggtggc aaggtatcga
    12901 cccaattgct gcgattcgca tattaggcca agcaaatgca attcatcact tccatgctaa
    12961 agatacgtat attaatcaag aaaatgtaaa tatgtatggt ctaactgata tgcaaccata
    13021 tggtaacgtt gcaacaagag catggacgtt ccgtacagtt ggttatggac atagtccata
    13081 tgtatgggca gatatcataa gccaacttat tattaatgga tatgattatg tattaagtat
    13141 tgaacatgaa gatcctatta tgtcagtaga agaaggtttc caaaaagctt gtcaaacttt
    13201 gaaatctgtt aatatttacg acaagccagc agacatgtgg tgggcataat acgaattcga
    13261 ggttagtctg aagtttgtct gaagtaagac tggtggcagt gttgaataaa tgcatatatc
    13321 gccaagccat tgccaaaaat ttcacacctt aaatcaagtt cattgtttgt aaagaaggtg
    13381 tactttatat aagtatatag cgatggtcat acccattcac aataacaatc ctcactattg
    13441 aaaagagtaa ataagctttt caatagtgag gtatatgata ataaaaaagc ctgttgtcac
    13501 aatggtcata gacacgacat actttaaagg tttctgaata taatatttca gaacgcactt
    13561 taaagatgga cgtcgatggc gactaatgtg atgacaggct ttcattttta aatattcatt
    13621 aatttctctt cttgtttaat acgtacatat aagaaatacg catacggtac taataaaata
    13681 gttgtatatg ttgcgtgtgt taataataat acaccgatta attcaggaat gatgtttaag
    13741 aagtaatttg ggtgttttgt aattttatat aatccagatt taataatagg atggttaggt
    13801 aaaatgaata attttaatgt ccaaatacca cctaaagttt taataaccat aaataacatg
    13861 atataagcaa agattaatat aactaagcca ataccatttg caaagctaaa tgtatcttta
    13921 ttaataaatg cctctacacc agccaataca taaattaaaa cgtgtgttat tgctaaaaac
    13981 ttcgaatttt taacgccata ttcaactgca ccgtctgctt ttaattgttt tgagtgatta
    14041 atagatatct ttaagctgac aagtctgata cagaaaaaga taagtaatat agatagaatc
    14101 atgatgtcct ccgtcattat gtcatatgta taagcgttga ttttgacaac ataaagtatt
    14161 ttatagataa aacttgtcaa atactattaa ctatttatta attttagtac ataaatatgt
    14221 ttctaagtat gtgtttatgt tcagtatttt ggataattta ataattttaa ggatattaag
    14281 cgcttacacc gacgtgatat atttgactta acgaaaatga ttgaggtgac agagatgaac
    14341 ttttttgata tccataagat tccgaacaaa ggcattccat tatcggtaca acgtaaatta
    14401 tggcttagaa acttcatgca agctttcttc gtagtgttct ttgtttatat ggctatgtat
    14461 ttaattcgaa acaactttaa ggcggcacaa ccgtttttaa aagaggaaat tggattatct
    14521 acattagaac ttggttatat cggattagca tttagtatca cgtacggttt agggaaaaca
    14581 ttacttggat attttgtcga tggacgtaac acaaaacgta ttatctcatt cttacttatc
    14641 ttatctgcga ttacagtttt aattatggga tttgttttaa gttactttgg ttcagtaatg
    14701 ggattattaa ttgtactttg gggacttaac ggggtgttcc aatcagttgg tggacctgca
    14761 agttattcaa cgatttcaag atgggcgcca agaacgaaac gtggccgata cttagggttt
    14821 tggaatacat cacataatat cggtggtgcc attgcaggtg gtgttgcact ttggggtgct
    14881 aatgtattct tccatggaaa tgttataggg atgttcattt tcccatcggt gattgcatta
    14941 cttattggta tcgcaacatt atttatcgga aaagatgatc cagaagaatt aggatggaat
    15001 cgtgctgaag aaatttggga agagccggtt gataaagaaa atattgattc tcaaggtatg
    15061 acaaaatggg agatctttaa aaaatatatc ctgggaaatc ctgttatatg gattctatgt
    15121 gtttcaaacg tctttgtata cattgtacga atcggtattg ataactgggc accgttatat
    15181 gtgtcagagc atttacactt tagtaaaggc gatgcagtta atacgatatt ctactttgaa
    15241 attggtgctt tagttgcaag tttattatgg ggctacgtat cagacttatt aaaaggtcgt
    15301 cgtgcaattg tagctattgg ctgtatgttt atgattacat ttgttgtctt attctacaca
    15361 aatgctacaa gtgtaatgat ggttaacatt tcattgtttg cattaggtgc gttaatcttt
    15421 ggtccgcaat tattaattgg tgtatcattg actggttttg ttcctaaaaa tgccatcagt
    15481 gtagcaaacg gaatgacagg ttcattcgcg tatctattcg gtgactcaat ggcaaaagtt
    15541 ggtttggcgg ctattgctga tccaacacgt aacggtttaa acatctttgg atatacatta
    15601 agtggatgga cagatgtttt catcgtcttc tatgttgcat tattcctagg catgattcta
    15661 ttaggaatcg ttgctttcta tgaagaaaag aaaattagaa gtttaaaaat ctaatatcaa
    15721 tcggattaaa agtatcgcca atttattgca gtatagttgg caatcctgcc ccgacggcat
    15781 gtgcgtgaag agatgaaaga tactgcttct acccttgcaa atatatcatc tctatgtctc
    15841 ggggcagatc ataattccct gttatgaagt atccttattt gcccgactta gggtgactca
    15901 atgaatttac tccctacaat aaagacatat agcggtgtca atattgtagg gagtattgtt
    15961 ttatatttag actctctaaa aagcggattg aaagaaaagt gaaaacttct ctatcagtcc
    16021 gctttttcat agaacaaaat ggatgcgcca taatcattag ttatttgcta atctattttg
    16081 cttgcttaca ataatcactt ggcgacattt gtaaatattt tttaaaatga tagctaaaca
    16141 ttttatactc tgaaaagcct actttgtctg caatttcata gtgcttataa tgtcgatcta
    16201 acaattgtag agattgtaaa atacgatagc gatttaaata atcgacaatt gtaataccaa
    16261 catgatcttt aaatgttcgc atcgcatacg attcactaac atcgatatgt tgaattaaat
    16321 ctgaaacagt cactttcgtt tgataagatt gcttaatttg atcaacaatc tgatttacat
    16381 aataatcatc gtattctact tttaataatg gttggaaggc atcatgacaa ggtgctaagc
    16441 tacggccgtt ctgtgattgt tgctctaata aggtacggac aagtcttcct aaaataactt
    16501 ctaattgtgc atggtctact ggttttaata aataatcaag aacatgatgt tgaatgccgg
    16561 ctttcatata ttcaaagtca tcgtaactcg ataat
//
LOCUS       DAMZPP010000060        16457 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMZPP010000060 DAMZPP010000000
VERSION     DAMZPP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16457
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.060"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..512)
                     /locus_tag="POH73_001428"
     CDS             complement(<1..512)
                     /locus_tag="POH73_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6887388.1"
                     /translation="MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTMISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSG"
     gene            complement(525..2123)
                     /gene="cntA"
                     /locus_tag="POH73_001429"
     CDS             complement(525..2123)
                     /gene="cntA"
                     /locus_tag="POH73_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HDI6887389.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            complement(2266..3567)
                     /gene="cntM"
                     /locus_tag="POH73_001430"
     CDS             complement(2266..3567)
                     /gene="cntM"
                     /locus_tag="POH73_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HDI6887390.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKEKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFSQDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMAILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGEMLGIKTPMINQFLTRYEA
                     SCKTYKDMHQDQQLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            complement(3560..4378)
                     /gene="cntL"
                     /locus_tag="POH73_001431"
     CDS             complement(3560..4378)
                     /gene="cntL"
                     /locus_tag="POH73_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HDI6887391.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEVLETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            complement(4389..5210)
                     /gene="cntK"
                     /locus_tag="POH73_001432"
     CDS             complement(4389..5210)
                     /gene="cntK"
                     /locus_tag="POH73_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HDI6887392.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVSQVTTE
                     IQHLVETYVREQQWNHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            complement(5911..6678)
                     /locus_tag="POH73_001433"
     CDS             complement(5911..6678)
                     /locus_tag="POH73_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6887393.1"
                     /translation="MNIRHIRNATAVIEYGGKRVLIDPMLSDKGAFEPFPNSPRQDEN
                     NPLVELPLAIEDIINNIDLVILTHLHIDHFDPKAIEVLPKDIKIYTQNEADASEVEGY
                     DFTNVSVFNDVTHIGEIELIKTDAQHGHGEILKMTGHVHGLVLKHSEEPTLYLAADTV
                     WFDGVEKTLKAHHPDIIVLNGGANQFFEGGPLVMDEHDVLKVAHTLSLAQIVVVNMEA
                     VNHWHLSREELNEFINSNDLGNRVVVPNDGELLTFEK"
     gene            complement(6779..7597)
                     /locus_tag="POH73_001434"
     CDS             complement(6779..7597)
                     /locus_tag="POH73_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6887394.1"
                     /translation="MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSE
                     TVDKIKSNGDKAKAYIVDIASEQQIDNFASEIREQIGHVDVLFNNAGVDNAAGRIHEY
                     PTDVYDKIMNVDMRGTFLMTKMMLPLMMTKGGSIVNTSSFSGQAADLYRSGYNAAKGA
                     VINFTKSIAIEYGRDGIRANAIAPGTIETPLVDKLTGTSEDKEGKAFRENQKWMTPLG
                     RLGKPEEVGKLVVFLASDESSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE"
     gene            7832..9370
                     /locus_tag="POH73_001435"
     CDS             7832..9370
                     /locus_tag="POH73_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDI6887395.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAMMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     VSVVVLLPTILLVTTKLIIPRLGQYDDRLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIAWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            complement(9482..9904)
                     /locus_tag="POH73_001436"
     CDS             complement(9482..9904)
                     /locus_tag="POH73_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDI6887396.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDKL"
     gene            complement(10357..10770)
                     /locus_tag="POH73_001437"
     CDS             complement(10357..10770)
                     /locus_tag="POH73_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6887397.1"
                     /translation="MSKKHVFIIIGVILCICIVATVIHFKMKYDEKEKQKAIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTKLETSPMGSAVIEGYINENKKDDFVAYASPENNFQ
                     FVGDIILSKNLSEIIKIKTKSPDEIKEELDKKEGR"
     gene            complement(10788..11207)
                     /locus_tag="POH73_001438"
     CDS             complement(10788..11207)
                     /locus_tag="POH73_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6887398.1"
                     /translation="MSKKKILILTNIMLIILISVAGIYFKMKYDEKEKQKSIYYKEQQ
                     ERITLYLKHNTIEPNTIKSVHFTNLETSPMGSAMIEGYINENKKDNFTAYATPEHNYQ
                     FGGAMIESERLSELLKPAQKLKSPDDIKKELNKKKGH"
     gene            complement(11459..11885)
                     /locus_tag="POH73_001439"
                     /pseudo
     CDS             complement(11459..11885)
                     /locus_tag="POH73_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
     gene            complement(11912..12322)
                     /locus_tag="POH73_001440"
     CDS             complement(11912..12322)
                     /locus_tag="POH73_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6887399.1"
                     /translation="MLKKKILMLTSIILIILISVASIYFKMKYDEKEKQKSIYYKEQQ
                     ARITLYLKHNTIEPNTIKSVHFTNLETSPMGSAVIEGYINENKEDDFVAYASPENNFQ
                     FVGDLIKSERLSELLKPAHQLKSPDDIKKELNKK"
     gene            complement(12600..13022)
                     /locus_tag="POH73_001441"
     CDS             complement(12600..13022)
                     /locus_tag="POH73_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6887400.1"
                     /translation="MTKKHVFIIIGVILCICIVATVIYLKVKYDEKEKQKSIYYKEQQ
                     ERITLYLKHNTKEPNTIKSVHFTSLKTGPMGDAVIEGYINENKKADFVAYGSPEHHYQ
                     FGGSLIKSKNLSTLLKPAHQTKSPDEIKKELESKKNDR"
     gene            complement(13274..13969)
                     /locus_tag="POH73_001442"
     CDS             complement(13274..13969)
                     /locus_tag="POH73_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6887401.1"
                     /translation="MTVLTDKIAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKDKLQ
                     NVANQLAQDSVKVVPTDVTKKEEVDELIKMAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAKAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITATYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
     gene            14268..14549
                     /locus_tag="POH73_001443"
     CDS             14268..14549
                     /locus_tag="POH73_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6887402.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEIKKLDDVRMPFSSAHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            14485..14668
                     /locus_tag="POH73_001444"
                     /pseudo
     CDS             14485..14668
                     /locus_tag="POH73_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14807..14998)
                     /locus_tag="POH73_001445"
     CDS             complement(14807..14998)
                     /locus_tag="POH73_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887403.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            15433..15555
                     /locus_tag="POH73_001446"
     CDS             15433..15555
                     /locus_tag="POH73_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887404.1"
                     /translation="MSYIIKFTFKFFEMTTYISQLTLDIELVSSELLVILSGFI"
     gene            complement(15559..16353)
                     /locus_tag="POH73_001447"
     CDS             complement(15559..16353)
                     /locus_tag="POH73_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6887405.1"
                     /translation="MIQSKKLTLGICLVLLIILIVGYVIMTKTNGRNAQIKDTFNQTL
                     KLYPTKNFDGFYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNT
                     RTTKGYYFISEMTDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLKKEIENLKFFV
                     QYGNFKDINDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLK
                     GDGDLKGSSVGSKNIEFTFVENKEENIYFTDSVQYTPSEDTRYESN"
BASE COUNT         4928 a         3012 c         2456 g         6061 t
ORIGIN      
        1 aatccagaag tcggcaatat gtttaacttc actgaaacgt aaataataag tattgaagct
       61 atccaatatg atggtaatgc agttagaaag aaagccactg aacgtatcgc acgatcagtg
      121 aactttcctc tttttaatgc actaactaca cctaaaataa ttgatgtaat catcaccata
      181 acacttgaaa tcattgttaa tttcaatgta ttcataaatg ctggaccaat gcgttcagca
      241 acaggatcac ctgtaatgta gcttgtacca aaattaaatt gcatcgcttc aagtaaccaa
      301 tttttatatt gaattaataa tggatcattg aaaccgtact tctcattcgt ttctgcaatc
      361 aactctggtg ttacatttgg cgttccttgt gcatgtaaaa ttgtcacagc tggattttca
      421 tttgtaatat acgtcaatag aaatgtcata aaacttacta caatcatcaa tggaaacatg
      481 agcgcaatac gttttaagat aaatttgaac atctaaacgc tcctttattt atactgcatt
      541 tcattgaatg gtaattcata ctgtgattgt gtgaatgata ctttttctaa atctttcggc
      601 gcaacaactg tcatactacc gtgtgaaata gggataaaaa taccttcatc atcaatttgt
      661 ttcaaaatgt ttttataagc gtctgaacgc tctttaccgt tttggatttt aaatgcgtca
      721 tcaatgctgt tgtatatttt atctttgttc tcaatgcctg atgttgcact ttcataacca
      781 tttttcgctt taaatgctgc aatagtactt tgtggatcgt acaataatcc ccaagtttgg
      841 ttgaacatta agtcataatc accagaagta cgacgttcag caattttatc tgatgtttcg
      901 ccattgatgt ttaacttaat acccattttc ttaaattctg cttgtaagta ttctgcttgt
      961 tctttttgac ttgaagaacc tttgtcatag tacattgcca tttcaaggtt tttaccatct
     1021 ttttgacgaa catcgctgtc tttacctttc ttccaaccag cttcatctaa taatgattct
     1081 gcttttttaa ggtcatactt acgtgttggc atatcgaaat taatgtctgt tacatttttc
     1141 gcaaataatt gagttgctgg tttttcttga ccatctaaaa tttctttggc aattttatct
     1201 ctgtttacca tatgaccaat cgcttgtctg actgttttgt cactgacagc gttatctttt
     1261 ttaccagaat tgaccactaa cattttcgta ttcataggtt gactacgctt aacttgatag
     1321 tcacctgtat ctttcaattg ttttaaagag tctttgtcta agctatctgt acctctatcg
     1381 tctgtgaagg caaagtttgt ttcacctttt ttcattgata ggaatgctgt ttcaccagca
     1441 ggcattactt ttgcttgtac tttgttaagt ttagactttt cgccccagta ttgatcattt
     1501 ttattaaagt ctgcagactc atcttttttg tgttcaccta atttaaatgg accagtacca
     1561 tcgaactttt taacgccatc ttttgttgtg ccgtttttaa agtcttttgg agatacaaat
     1621 acatatggac gaggcatcgc taattcagcc aatgcaggtt gatatgcttc tttcaaattt
     1681 aattcaaccg tgtacttatc tttaacttta acattgtcaa ttaatgttga aatctttaac
     1741 caagaatgca attttttgtt ttcttgaact gcgtcaatat ttttcttaac tgcgtcagca
     1801 tcaaatggcg taccatcatg gaatttaacg tcatctctca aatggaacgt gtatgtcttc
     1861 ccatcttctg acacatccca ttttttagct agtaaaggct taatcccatc tttcgtgtta
     1921 cgtacaagcg gctcgtatat catactttca gcagacatcg atccaccgta aacatgcgga
     1981 ttcatatccc cgatatcttt aaccgtcgta tacgttaatt gcttgttttc ttttttctcc
     2041 tctaaacctt tattaccgcc acaaccagtt aaaattagcc ctgatgcaag taacattgca
     2101 ctcattttag ttagttttct catttgcttt tcctctttct aaattgataa gttgcattac
     2161 ttaatcaaat cgtaatgatt atttttctca attatttgct tatttttatg taaatgtcaa
     2221 acataattca ataatttttt tctattataa aacttcaaac ccttattatg aaagcgttct
     2281 attgatttcc aaaaattttg tgacgagtgc cttatctcct tcaaatagat ttgtattaaa
     2341 ttgagaagat agttgttgat cttgatgcat atccttgtac gtcttacaac ttgcttcata
     2401 gcgagttagg aactgattaa tcattggcgt tttgatacct agcatttccc caatatgctg
     2461 aatcatcgct gttctgtaat aatcctcact tggcattctt ggaatttgaa caacatcctg
     2521 ttcattttta tagacttgct taaatggtac agctgaaaaa tcaaagtaat gtccgttttc
     2581 gtctggctgt gaaaatggat caataaggat tgcggtatat cttacataaa gcagatattc
     2641 ttgtaagata tctggcaaga tttcgaaatg ctcaatatca ccttcatcca aagtttcagg
     2701 acgtactgga taattttcct tcaccataaa ttgaagcagg ttgactgacg gtactctaaa
     2761 tgcttgtaaa atagccatca tttccttcca cattaaacgc atttcacgga ttagtgtcat
     2821 cgttatcggt ccttcaggaa ataacttata cacataaacc ggtacatccg ttccttcgaa
     2881 aatggctttc aatgaaaagt cattcataaa tagtggtggg tgcacataaa gcgagctgtt
     2941 tcgcgtttca gcatgcagtg gcgattcaac gacttctaat tgaatcttca actgctcagc
     3001 taaagcagag attcgttgac acattgttga gtttgaatgt gtcgatccca tgtacaattt
     3061 cttttttaca cctgttgtca acacatgatt aggtgcttct ttatcaacaa tacgtgtatc
     3121 gccaagataa gttgaaaatg aaatcacttc gatatcctga ctgaacttag acataaattg
     3181 ttccacaatc atttgcgatc caaatgtcgg tgaaattaaa atgacatgtt tgacactttg
     3241 caaagtttct aacgacaatt gctgtagtgt gtcataataa gcatctgctg tgcatgccat
     3301 gacaaccgtt tcgtattcac ccttaacgtt tttaacatct ttatacaaac gattaatttc
     3361 aaacttacct tccagatgtt gatgcgcctc gttttgtatt ttgacttcaa attgtttctc
     3421 ttttttatac gcttgatata agcgttttga tttttctgat gttgaggcac gtccaaccat
     3481 atcaatctca taatctgatt ttaaatagca aatattcgct aattggattg cgaccggacc
     3541 agtgcctatc attaataatt tagacatccg taatacctac ctttatagct gcttttttat
     3601 aaagtgctat atcaaaaatt tgctgtggtc tcatatgttt attcacacat tgccacttat
     3661 cttccgctgt ttcttgtgac ggataattaa atattgcttt gatgccatca ccaaagcgca
     3721 ttgcaactac gacattttca tttgttaaat catataattc ttctaaaatg ctgtacttta
     3781 aaggaattgt cgagctgaat atgatatgcg tcacatcttt gatatcttta agttcagata
     3841 ccttttgatc cgtaattgtt atatcttcat ttggtgctaa gacgttaacg attctgcgtc
     3901 ctaggtcaac ggcttgtgga tcaatatcaa taccgataac tgaagcacct gtttcttttg
     3961 ctacttgaat taacgtcatt ggatatgcac ctgaaccaac taacaataat ttgtcattgc
     4021 tagctatact acattgtcca aactcttcat caatacaatg ttctatattg tcaaagtaac
     4081 ctgtggtaga cgcctgtccg tctaatagac gtctagctct aatgacttcc acttgtttaa
     4141 cacactgcgc tgtaatatat tgcagtgatt ttattaattg tgctttttct tcaacgtcac
     4201 gccaagcatt aaattgttgt tcataaatag gatttaaaat aaattcacta tagtcatcca
     4261 tcaatgtttc taatacttcg atatattgat cgtcttgtaa tacacgctcg taatgcgctt
     4321 caaacttttc taaatattgt tgtaatatca atttgatttc attattaaag ttattcattg
     4381 taggctcctt attctatata tgcttttcct gtagctacag ttgtaacctg tcctttaatt
     4441 gaagtttgat atcccaattg atgacatcgc tttgatgtca ctaaaatact gccccctggc
     4501 tgatgtactg taaaatcttt gcatgcgtca ttacgttgat aattattaaa aaccccaatt
     4561 gatgctgtac cagaaccaca gctattttcc caaattaaac tttggatttc tggtatatag
     4621 attaatggct gtaaaaattg acgttgttca tcaaaaagca tcatacctac tgttttatat
     4681 ttgtgattcc attgttgctc acgcacatat gtttcaacca aatgttgaat ttctgttgtt
     4741 acttgactaa ctggaatcac ataatgtaca tatgtttcat aaataatttc tattgctttc
     4801 catgaatgat tacccatatt aattgttgtt ggcacaacac gatgggcttg tggcatttga
     4861 acttcatagt attggcagtc atgaattgcg cattgcacta aatccgaaca gccagatacc
     4921 ttcaccttaa actgttggtc tttaagcaaa tgactttcct gcaaatgatg tatatatgac
     4981 atcgtcgcat taccgcaaaa ttcattaccg ctcataacta agtgaaaatc attgccatca
     5041 tcattttgtg ttgattctat aaagcctacc tgttcacaac atacatgtgt tgcggccatc
     5101 aactgattgg cgatagatgc atattcacta gcatcatgtt ttgaatgaac aagtatcgtc
     5161 atattccccg aaggattata cttagaaaat tctataacct gccgattcat acgacactcc
     5221 tttagatgta tttttagagc ctctgtaatt ttaattacta gaggctctta tgcagttgtt
     5281 gcgaagcaag caactgtatt cctctagctc ttttacctta agtgattttt cagaagatga
     5341 acctaatagt ttcccatcta ttaggtaagt catgctaatc cgcaaaagcg aaaagcatgg
     5401 ctttatatgc agttgttgcg aagcaaacaa ctgtattcct ctatacctgt aattttttag
     5461 aagatgaacc taatagtttc ccatctatta ggtaagtcat gctaatccgc aaagtggaaa
     5521 gcatgactta gtaatcggtt gttgcatcaa catgcaaccg taaaccttat ttaaagttgc
     5581 ttcttgtggc tctacaagcg aatgcagttg tccgttaact acattcactt agatgctttt
     5641 actgttttca cctcaactaa aacttcaaaa aagttgcaga aaacaaatcg caactattac
     5701 gatttgatta tatactatat tagttgagaa tcattgtcaa taaaggttgt aaatgaaagc
     5761 acatttatat tttatgaatc cctccataaa catcgcattc tcagcacatt caagtctttt
     5821 tgaaattttt atcaaacaaa aaagccagct tcaacgtaaa atacgttaaa actagcttta
     5881 agcaatacat ttcatcaaat aaacaaatat ttatttttca aaggttaata attccccgtc
     5941 atttggtaca acaactctat tacctaagtc gttagaattt ataaattcat tcagctcttc
     6001 acgagataaa tgccaatgat tgaccgcttc catattcaca acaactattt gagcaagcga
     6061 caatgtatga gcgactttca atacatcatg ttcatccatc actaacggcc cgccttcaaa
     6121 gaattgattt gcaccaccat taagtactat gatatctgga tgatgcgctt ttaaagtctt
     6181 ctcaacaccg tcgaaccaaa ccgtatcagc agctaaatat aacgttggct cttcagaatg
     6241 tttcagcact aaaccgtgaa catgtcctgt cattttcaag atttcaccat gcccatgttg
     6301 agcatctgtt ttaatcaact caatttctcc aatgtgtgtc acatcattaa ataccgaaac
     6361 gtttgtaaag tcataaccct ccacttcact agcgtctgct tcgttttgtg tataaatttt
     6421 aatatctttt ggcaatactt caatcgcttt cggatcaaaa tgatcaatat gcaaatgtgt
     6481 taaaattact aaatcgatgt tgttaatgat atcttcaatt gctaatggta gttctacaag
     6541 tggattgttt tcatcttgtc tcggtgaatt tggaaatggt tcaaatgcgc ctttgtcact
     6601 tagcattgga tcaatcaaaa cgcgtttacc tccatactca atcacggccg tggcatttct
     6661 tatatgtcta atattcatgt taaatccccc tatttcatat gtctgtcatt gcgacaatat
     6721 gcatacattg cctacttcat gccaaattat tttccactgg atatcgattt taaaacaatt
     6781 attccaatgt ccgcttccac gaatcatcac tcaacatctc accaggccat gtgtaagcca
     6841 taacaccacc gtcaatgcga attgtttcac cagtaataaa tgaactctca tcagatgcta
     6901 aaaagacaac gagcttgcct acttcttctg gtttgcctaa acgtcccagc ggtgtcatcc
     6961 atttctgatt ttctctaaac gctttgcctt ccttatcttc gctagtacct gtcagtttat
     7021 ctactaacgg ggtttcaatt gtacctggtg caattgcatt agctcgaatg ccatctctac
     7081 catattcgat tgcaatggat ttagtaaaat taatcactgc ccctttcgca gcattatatc
     7141 ctgagcgata caaatctgct gcctgtcctg aaaatgaaga tgtgttaaca atagagccac
     7201 ctttagtcat cattaaaggt aacatcattt tcgtcatcaa aaatgttcca cgcatatcta
     7261 cattcataat cttgtcatat acatctgttg gatactcatg aattctacca gccgcattat
     7321 ccacaccggc attattaaat aacacatcaa catgcccaat ctgttctcta atttctgatg
     7381 caaaattgtc aatttgttgt tcgcttgcaa tatccacaat ataagccttt gctttgtcac
     7441 cattactttt aattttatcg acagtctccg ataccgcttc agctatgtct accgccaata
     7501 catacgcacc ttcttgagct aaagcgattg cagaagcttg tccgatacct gtacttgctc
     7561 ctgttacgac tgcaacttta ttttccaaac gtttcattca gtcactccta tacaacatat
     7621 cttaaactta cagtccttat tttccactgt tcaatgcttc ataaacctaa tagctaagtc
     7681 tccaggcagt cttagctact ctctgctata caatttctat tgaataactc aaaatattta
     7741 acaatattag atttttcatt ttctgaaaat tgttactttt gagagtattt tttgttacac
     7801 tttaaatatt taacaaaggg gggaatcatt gatgacatca aaacatcaac aaaaggggtc
     7861 tatcgtcaat cgtttcttga atagtgttga aaaaattgga aataaattgc cagatcctag
     7921 cgttttattc tttttaatgt gtgtgggctt agccattatg acgtgggtta tctcattatt
     7981 taatgtatct gttaagcatc caggtacgca tcaaaccatt tatattaaaa atataattag
     8041 ccatgatgga tttacaatga taatgaatga tacgattaaa aatttctcag agttcccagc
     8101 attaggctta gtactagcaa tgatgattgg tataggtgtt gcagagaaaa caggatactt
     8161 cgacaagtta atgatttctg ttgtgaatcg cgcaccacgt ttcttaattt taccgactat
     8221 tatattaatt gggattttag gtagtacagc cggcgatgct gctacaatta tcttgccgcc
     8281 gctcgcagcg atgcttttta ttaaaattgg ctatcaccct atcgctggac taacgatggc
     8341 atatgcttcc gctgtaggtg gctttgccgc aaatatagtt gttggtatgc aagatgcttt
     8401 agtctattca tttacagaac cagcaacacg tatcgtttca gattctatta aaacaaatgt
     8461 tgctatgaac tggtacttta tcgcagtgag tgtcgttgta ttacttccta cgatattgct
     8521 agttacaact aaactgatta ttcctaggtt aggtcaatat gatgatcgtt taatgcatga
     8581 tgatcatgaa gaaacgtctt cacatattac tgacaaagaa gcacatgctt taaaatgggc
     8641 aaatatcagt ttcatagtca caattatttt attaattatt acagccattc ctgaacatag
     8701 ctttttaaga aatgctaaaa caggcagctt actagacgat gcgccattaa ttaacggtgt
     8761 cggattaatt attcttgttg tatttttagt acctggatta gtttatggga ttttaagcaa
     8821 agaaattaaa aatacaaaag acttaggtaa aatgtttggc gatgctgtag gatcaatggg
     8881 aacatttatc gttattgtgt tttttgcagc acagctactt gcgtatttaa agtggagtaa
     8941 tttaggaatt atcgctgctg ttaaaggtgc caaattatta gaacatcaaa acggtattgt
     9001 attgatacta ggtattattg tactgagtgc aatggtcaac atgttaatcg gaagcgcatc
     9061 ggctaaatgg ggtattttag gaccgatatt cgtaccaatg ttaatcctta tcggctttca
     9121 ccccgcattt acacaagtca tttaccgtgt aggagattca attaccaatc caattacacc
     9181 gatgatgccg tacttacctt tactattaac ttatgcacaa aaatacgata aacgcatgaa
     9241 actcggagcc ttactttcta gtttaatgcc gtattcaatc gcgctaagta ttgcgtggac
     9301 agtatttgtc attatttggt tcttattagg tatccctgtt ggaccaggtg gaccgatatt
     9361 cgttaaataa cagcactcaa ggggataatt taaaatgcca ctatacaaga tttgacctgt
     9421 atagtggcat gatttgttat gatatagagt tcgagatgaa actctgtatt tatatgaaca
     9481 attatagttt atcactaata atatttagtc tgttccaacc gttaatttgg tttacaacaa
     9541 agacaagatc aataatttgt tcttcatcat aaaatgattt tagccggtca ataatttcaa
     9601 acttcttaaa gtctttgatt gaatttaaca tttctgcaaa ttcaagcgtc actttttctt
     9661 gatcagtaaa taaatctaaa tgtctaaaac tcaagacttc atcaattttc atttgtgtta
     9721 cacccaattc ctttaattct tttttatgga tatcaacaca ataatgacaa ccgttaattt
     9781 ttgaaattaa taacttaatc aatgcactaa gttcatcatt aacgggtgat tgatgaataa
     9841 acttttcacc ttggctcatt acagacatta actctccatt ttgtttcttg taattatacg
     9901 gcatttgcaa tctctccttt acattgttta ataccaatcc agtgatacta cacttcgaac
     9961 aatattggat agaatttgaa attctgtata acatcttgtc aatctgtgct ttagttaaaa
    10021 catttagcgc ttatatttaa aaaatatata aaatatgaaa acagattgaa aggatattat
    10081 accttgctct accctttttt ataaaaaatc acacacattt gagaaaattt gataatgtca
    10141 ataaatcacg tcatcgccat tgttattcgg tatataatgt ttttataatg gccaaactat
    10201 tatttcattt atattaatag ggttcacttg tgttaatgta ctgtaaaaaa actggcgaag
    10261 tattggtgat gatgaagtga tttatattaa ttgcgaaaca ccttcgatag caatgaaata
    10321 gattattgga agattaaatc aaaaataaag aagaccctag cggccttctt ttttgtctaa
    10381 ttcttctttg atttcgtcag gtgattttgt ttttatttta attatttccg ataaattttt
    10441 gcttagtatt atatcaccta caaattgaaa gttattttca ggcgatgcat aagcaacaaa
    10501 atcatctttt ttgttttcgt ttatgtatcc ttcaatcact gcacttccca taggacttgt
    10561 ttccaacttt gtgaagtgta cagatttgat cgtattcggt tctttagtgt tatgcttgag
    10621 ataaagtgta atacgcgctt gttgttcttt ataataaatt gctttttgct tttctttttc
    10681 atcatatttc attttaaaat gtatgaccgt agcgactata catatacata aaataacacc
    10741 aataattata aaaacatgtt ttttggacat ttaaccacct ccaactttta gtgacccttc
    10801 tttttgttta gctctttttt tatatcatca ggcgatttta atttctgtgc tggttttagt
    10861 aattcactta atctttcact ttctatcata gcgccaccaa attgataatt atgttctggt
    10921 gtagcataag cagtaaaatt atcttttttg ttttcattta tgtaaccttc aatcatagca
    10981 cttcccatag gacttgtttc caagttagtg aaatgtacag atttgatcgt gttcggttct
    11041 atagtattat gcttgagata aagtgtaatg cgctcttgtt gttctttata ataaattgat
    11101 ttttgttttt ctttttcgtc atatttcatt ttaaaataga tacctgcaac actgattaat
    11161 atgattaaca taatattagt taatattaaa attttctttt tcgacatgta tcaccagctc
    11221 ctaatgtaag gtggaaaatg aaaaattttt atagttctgt aataattaat gttatagttt
    11281 ataaatcata gtaccactat cgtcgtatcc aacaacgtga atattagaat taactattac
    11341 tgagaattga acgatatgat attccgcttc tataaaattc gacaccctac taacataaat
    11401 atcttcgata gcaatcgata ttaaacatgt acaaatcaag ctaaatatta cgtgaaaatc
    11461 actgtttagt gtttttcttt ttgtttattt cttctttaat tttttcagga gattttaaat
    11521 tttcagcgtt cttcaatttt cctaagactt cattacttcc tgtcaatcct ccagtgaatt
    11581 gatagttttc ttcaggtgac ccatatgcaa caaaatcagc ttttttgtgt tcattgatgt
    11641 atccttcaat tactgcatcg cccataggtc ctgtctttaa acttgtgaaa tgcacagatt
    11701 tgatggtatt gggttctttg gtattatgct tgagataaag tgtaatgcgc tcttgttgtc
    11761 ctttgtagta aagtgctttt tgtttttctt tttcgtcata atttcatttt taaataaatg
    11821 actgatgcaa ctatacatat acataaaatg acaccaataa ttataaaaac atgttttttg
    11881 ttcattaaag taactcctaa ctttagtgac tctacttttt atttagttct ttttttatat
    11941 catctggtga ttttaattga tgcgctggtt ttagtaactc acttaatctt tcacttttaa
    12001 ttaaatcacc tacaaattga aagttatttt caggtgatgc ataggcaaca aaatcatctt
    12061 ctttattttc atttatgtaa ccttcaatca cagcacttcc cataggactt gtttccaagt
    12121 ttgtgaaatg tacagatttg attgtgttcg gttctatagt attatgctta agataaagtg
    12181 taatgcgcgc ttgttgttct ttataataaa ttgatttttg tttttctttt tcatcatatt
    12241 tcattttaaa atagatactt gcaacactaa ttaatatgat taatataata ctagttaaca
    12301 ttaaaatctt ctttttcaac atatatcacc agctcctaaa atgtggtggg attttataca
    12361 gtttacatgt ttatgatact acataacacg ctcttacaaa gaaaaactat aattttaatc
    12421 taggtcaagg aaaagcaatt aaaagtgttg atataaagtt catctaagat catcggaata
    12481 ttgcggttat gaagtggtta acagataaat tcagtggaaa ttgtactaaa aattcaactt
    12541 ttcattttta tgtgtaagta tgattaactt gaaagcaaaa ttaaaacgac tgcatagttt
    12601 tagcggtcgt tctttttaga ctctaattcc ttttttatct catcaggtga tttggtttga
    12661 tgtgctggtt ttaataacgt gcttaaattt ttacttttga tcaaacttcc accaaattga
    12721 taatgatgtt ctggagaccc atatgcaaca aaatcagctt ttttgttttc attgatgtaa
    12781 ccttcaatta cggcatcgcc cataggtcct gtctttaaac ttgtgaaatg tacagatttg
    12841 atcgtgttcg gttctttagt gttatgcttg agatagagtg taatacgctc ttgttgttct
    12901 ttataataaa ttgatttttg cttttctttt tcgtcatatt tcacttttaa ataaatgact
    12961 gtagcaacta tacatataca taaaataaca ccaataatta taaaaacatg ttttttggtc
    13021 attaaagcaa ctcctaaaat gtggtggaaa atgaaaaagt ttttatagtt ctgtaattat
    13081 gaacattata gattataaat catagcacta atatcgtcgc cttcactcac tcgattgtta
    13141 aaatcaccag ttactgagaa ttgtgcaagg aataatttcc tatcatgctt ttatatcatt
    13201 attgccttac taatattaac aaaaccgata gctattacat taaatgccta cctcccacac
    13261 ctcaaacacc tttttataca ggacgcactg taatttcatt cacgttaacg tgctttggtt
    13321 gtgttaatgc atataatact gcttctgcaa tatcttgtgg ctcaagtttt ttacgatctg
    13381 ttggattgta tgtggcagtt attgctgtat caaccattcc tggagaaatg cttgttactt
    13441 taacacctgt ctttgccaac tctttttcta atccttgagt aatagtgtga actgccgctt
    13501 tcgtcgcact ataaatcgta ctgcttttcg ttacttcaaa gccagaaata gatgcaatgt
    13561 taataagatg gccacttgat tgttctaaca tagttggtaa tgcagccttt gccgtgtata
    13621 aagtgccttt gatattaaca tcaatcatac tatcccattc atctacttga taatcagtaa
    13681 tcttagacga caacatttgc cccgcactat tgatgacaat atccaaacca ccgaatgttt
    13741 gttgtgccat ttttatcaat tcatcgactt cttctttttt cgttacatca gttggcacta
    13801 ccttcacact atcttgagct aattgattcg ccacattttg tagtttatct ttatttctac
    13861 cggctaagac aactttcgcc ccttcttcat gtagtaatgt tgcaattgct tctccaatac
    13921 cactacctgc acctgtaact actgctattt tatctgttaa tactgtcata atgatcgact
    13981 cctttgattc tttttatttt tttagggtac atcataagta catattacta tttatacttt
    14041 taaaaagcgt atcacaattc atattctggt tatactaact cgatttattt acactgataa
    14101 agtacatttt taagttattc aaaatataca aataatcgtt gttcatattt tttattttta
    14161 aaaatgttga aaaattgtca attctttatt gtaaaagtcg aaagttagta ctaaagttat
    14221 aagtattagt aaacaatggc aaggctgtta gaaatggggg agttattgtg acatatcatg
    14281 agcgtgtgtt agcactgaga gcagaaagta aaagaactgc atttgatttt agattcgagg
    14341 atttatttag caaagaagaa tggctaagta tgtcgcttgc agaaagacaa aaagccgaga
    14401 aagcatttcg acacgaaatc aaaaaattag atgatgtaag aatgcccttc tcaagtgcac
    14461 atgatgccca agtgaaactg tataatgttg tgtattcata caatggcatt aaacgtaatt
    14521 tcaaacaagt tgaaaatgaa ggattctaat atcatttcgt ttatatatcg tggacatgat
    14581 acagttatat ataatcttgt aggtaatcat actaaaattc aatatagaca tgactaaagc
    14641 cacatccaat ataggacgtg gcttttagta tcgttccaga tttgtgggga gttagggttt
    14701 acttaattaa aggggtctgc cctcaattgc tcaccaacga gggcaagtta catcaatcac
    14761 acttcaattg ccgccgacta ggggtagtaa tcattggcaa taagaactag ttagtgacta
    14821 acgatttacg ttccatagca aagtgataca aacgctcagc atcaatgtaa ggcatagaat
    14881 caatagttag gtaaccatct ttgatgtcac caattaatcc ttgatttaca ccagtgttca
    14941 cgtaaatatc atagctactt acgtctgaga aaataagcgc ttcaatctct tttctcataa
    15001 tcatcactcc aatgtttata tattatttat ataaactctc tcgtctctct ctatttatta
    15061 acttcttaca agtctaatat tacatgaatt tctaaataaa taaaggggtt tgtgatgcat
    15121 ttcacaaatt tatcactatt ttggaaaatt aagaaatagt taattatata aaagtttaat
    15181 aagtcagaaa atatgataaa atgtagatgt tctttagaca ttaaaagctt ctaacatgat
    15241 acggaatatg aggttcctgt atcacattag aagctttttt gttgtggtgc ttatattgat
    15301 aatggcatga atgaacacac gctgtcttag tttcaggaat cggctagaat attaaacttc
    15361 cgttctttga atatttttta atttcttact aatatcatcc agttcattct tcgccttttt
    15421 aacccaatct ccttgagcta cattatcaaa ttcaccttta aattttttga aatgactacc
    15481 tatatttcac agctcacttt agatatcgaa cttgtttctt cagagttact tgttatatta
    15541 tcaggtttta tttgatattc agtttgactc ataccttgta tcctcacttg gagtatattg
    15601 tacagaatcc gtaaaataga tgttttcttc tttattttct acaaaggtaa attctatatt
    15661 ttttgatcct actgatgatc cttttaaatc accatcacct ttcaataata gtttcggcgc
    15721 ttgtttggtt ggaatatcat atctttttct taactgttgg acattataat catcattatt
    15781 caattgatat tttgccgaat aacttggtac attaggatta tatgaaatat caccatcttt
    15841 ataatcatta atatctttaa agttgccata ttgtacaaag aacttaaggt tttcaatctc
    15901 tttttttaac ttgtcattcg gtagtggctt cgttggtatg attttattat gttccatttt
    15961 taccggatac cttttttcat catcctttgg tctgccgtta ctgtcatctg tcatttcact
    16021 tataaaataa tacccttttg tggttctagt attgcgattg atatagagca ccattcctct
    16081 cgtttccata tccttacctt ttggctcgat taccatttca gaattaacta tccaagtacc
    16141 tttatcaccc tttttaaatt cttgatctcg aaagccttct ttatcgtaaa aaccatcgaa
    16201 atttttggtt ggatataatt ttaatgtttg attaaatgtg tctttaattt gggcgtttcg
    16261 accatttgtt tttgtcataa tgacataacc tacaatcaat ataatgagta ataccaagca
    16321 tataccgagc gttaatttct ttgattgaat catcatttct ccaccttata tttttaatac
    16381 tgactcaatc tttaatttat acatctaaag agaaaaattt tttaacactt aatggaattt
    16441 tcattccaaa tttctat
//
LOCUS       DAMZPP010000061        16195 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMZPP010000061 DAMZPP010000000
VERSION     DAMZPP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16195
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.061"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            23..739
                     /locus_tag="POH73_001448"
     CDS             23..739
                     /locus_tag="POH73_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6887406.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(1114..2073)
                     /locus_tag="POH73_001449"
     CDS             complement(1114..2073)
                     /locus_tag="POH73_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDI6887407.1"
                     /translation="MRRLFSIGEALIDFIPNITNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAHMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKSVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(2070..3554)
                     /locus_tag="POH73_001450"
     CDS             complement(2070..3554)
                     /locus_tag="POH73_001450"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDI6887408.1"
                     /translation="MTEWTREERYQRIEDVDTDYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYGEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKCDQFNNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILETPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            complement(3703..4653)
                     /locus_tag="POH73_001451"
     CDS             complement(3703..4653)
                     /locus_tag="POH73_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6887409.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTKIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHEINFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            complement(4837..6087)
                     /locus_tag="POH73_001452"
     CDS             complement(4837..6087)
                     /locus_tag="POH73_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDI6887410.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGEGNLWFGNWEYTFLNHVGFATQEDISPHIPLALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDQIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAFFQSKKAN
                     PDIENGFIYTGDVHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            complement(6296..6520)
                     /locus_tag="POH73_001453"
     CDS             complement(6296..6520)
                     /locus_tag="POH73_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDI6887411.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITVQKV"
     gene            complement(6580..7659)
                     /locus_tag="POH73_001454"
     CDS             complement(6580..7659)
                     /locus_tag="POH73_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDI6887412.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITVQSIGLLILTTTGILQIPAHSFPILGTVIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIAIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGAREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVQQQSAKVQQQTQIV"
     gene            complement(7965..8600)
                     /locus_tag="POH73_001455"
     CDS             complement(7965..8600)
                     /locus_tag="POH73_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDI6887413.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            8853..10781
                     /locus_tag="POH73_001456"
     CDS             8853..10781
                     /locus_tag="POH73_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDI6887414.1"
                     /translation="MILLQLNHISKSFDGEDIFNDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNLSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDWLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLHGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPISIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLQLALLMLQRDNVLILDEPTNHLDIDSKEMLEHALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHEKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANVLAIQKQDS
                     EQKLEHAMSKWEELQQKL"
     gene            complement(10926..11054)
                     /locus_tag="POH73_001457"
     CDS             complement(10926..11054)
                     /locus_tag="POH73_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887415.1"
                     /translation="MIKTMSIENMRTYQQRVYNLWIKGVDNVIYCGYQVGKQKIND"
     gene            11149..12759
                     /locus_tag="POH73_001458"
     CDS             11149..12759
                     /locus_tag="POH73_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HDI6887416.1"
                     /translation="MSTNQTFLIFVIAIIILTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLESFIRPNSHFDYEYKLYKDKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRHNIL
                     FLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHINELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELT
                     HPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPAKIYERAKDNVSNG"
     gene            complement(13073..14098)
                     /gene="tsaD"
                     /locus_tag="POH73_001459"
     CDS             complement(13073..14098)
                     /gene="tsaD"
                     /locus_tag="POH73_001459"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDI6887417.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITTTINEALVDADVSMEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLTFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(14091..14555)
                     /gene="rimI"
                     /locus_tag="POH73_001460"
     CDS             complement(14091..14555)
                     /gene="rimI"
                     /locus_tag="POH73_001460"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDI6887418.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDIERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            complement(14528..15190)
                     /gene="tsaB"
                     /locus_tag="POH73_001461"
     CDS             complement(14528..15190)
                     /gene="tsaB"
                     /locus_tag="POH73_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDI6887419.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIDALRAFLEDLNQPFIY
                     IGKDTVKLQDDLQGDVIAQLPNASVMYQLIDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            complement(15171..15632)
                     /gene="tsaE"
                     /locus_tag="POH73_001462"
     CDS             complement(15171..15632)
                     /gene="tsaE"
                     /locus_tag="POH73_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDI6887420.1"
                     /translation="MIKINNLDEMNQFAMFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENSRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            15670..15825
                     /locus_tag="POH73_001463"
     CDS             15670..15825
                     /locus_tag="POH73_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887421.1"
                     /translation="MIIHCSIVSNFIFNLYIVTNFNLNFLSLKQIISSKLIVRKFKIF
                     VDILKQI"
BASE COUNT         5468 a         2960 c         2288 g         5479 t
ORIGIN      
        1 ctagccataa ggatgtgaat gtatgaaaat tttcatttgc gaagacgatc caaaacaaag
       61 agaaaacatg gttaccatta tcaaaaatta tataatgata gaagaaaagc ctatggaaat
      121 tgccctcgca actgataatc cttatgaggt gcttgagcaa gctaaaaata tgaatgacat
      181 aggctgttac tttttagata ttcaactttc aactgatatt aatggtatca aattaggcag
      241 tgaaattcgt aagcatgacc cagttggtaa cattattttc gttacgagtc acagtgaact
      301 tacgtattta acatttgtct acaaagttgc agcgatggat tttattttta aagatgatcc
      361 agcagaatta agaactcgaa ttatagattg tttagaaact gcacatacac gcttacaatt
      421 attatcaaaa gataatagcg ttgaaacgat tgagttaaaa cgtggcagta attcagtgta
      481 tgttcaatat gatgatatta tgttttttga atcatcaaca aaatctcaca gactcattgc
      541 ccatttagat aaccgtcaaa ttgaatttta tggtaattta aaagaactga gtcaattaga
      601 tgatcgtttc ttcagatgtc ataatagctt tgtcgtcaat cgtcataata tcgaatctat
      661 tgattcgaaa gagcgtattg tttattttaa aaataaagaa cactgctatg catcggtgag
      721 aaacgttaaa aaaatataat aagataataa agtcagttaa cggcgtattc aattgtaaat
      781 cttgttggat tttaacaaga taactagcaa atgcactgta tagctggctt tttatttttt
      841 aatagcaaaa atttaattta aagcgtaaat ttaaataaaa cgatacaacg tttcattgta
      901 tacctattcc tattactaca taatttatca atttctattt cactctacaa tcctatagcg
      961 caatttttaa caaaatatac taaatttgaa atcgacagtt tccataaata aaatgctaca
     1021 atatcctacc caataaatct tattttgctc acaaaaaaaa cagtgtagca caagattcaa
     1081 cttgtttcct acactgtttt ataaatgaat tatttaaaat gcttgttcaa cctcttgtaa
     1141 tgttggcaaa ctattaatag caccatactt cgttgtcact attgcagcaa cgcgattgct
     1201 aaacgctaaa atttcctcac cttcattttc aaataactga gttaaattta acacatcggt
     1261 tgctaaaatc ctgctaataa ctgcaccaat aaaggcatcc cctgccccag ttgtatcaac
     1321 agattttacc ttatagccac tatgataatg attaatacca tttttcaaat atacggcagc
     1381 accatctgct cctttcgtat aaatgactac agtgacatta cctgtaaata atgattgaat
     1441 ggcttcattt tcatcatgaa tacctgtaat aaattctaat tcttcatctg aaacttttac
     1501 aatatgcgcc aatggcaaaa aggtatgaat agtttgtctt aaatcttctg cgttatccca
     1561 taatggcaat cttacattag gatcaaatac gacagtacca tttgcattta atgttttggt
     1621 aatcaattga taatgcgcat ctctcattgg actatcgacc aaatcaacag aacaaaaatg
     1681 tactacatcg ttttcattca catcaatatc attcacaaaa cttggttcaa ataacatatc
     1741 agcagagggc ttacgataaa aagaaaaatc tcgctctccc gcttcagtca aactcacaaa
     1801 agctaacgca gtgtttgctt cattcgtgcg atacactttt gatacatcta caccaatact
     1861 tgatatcgtt tcaataatgc tatctccaaa tgcatcatta cctaattgtg taatcatatg
     1921 cgcttgttgt cctaactttt gaactgtaca agctacattg catggcgcgc caccaatttg
     1981 cttcgtaaat gtttgaacat cttttaaatt cgcatttgta atatttggaa taaaatcaat
     2041 taacgcttct cctattgaaa ataaacgtct catttatgtt catcctttaa atcatattta
     2101 gtaaattgta aatatacttg ccctgattct gttgatgttt taatacctag tgcgtcttct
     2161 gtcgggaaaa ttcgagatgt caaaacacgt tccccatcat tacagaaaat ttctatacta
     2221 gatgtatcaa caaaaatttg taattgcttt aatggcgttt ctaatatcgt actacgcgtc
     2281 gtaccttcaa catttgtagg caataaacca ctgtcgctgc gatctaatgt tattttattt
     2341 tcacgcttgt tataagcgat taatgttgaa gaagtcttag acgtacgtaa ttcaaagtat
     2401 acttcggtag catcattatc caaaatatct atgattaatt catactgttt accttcatac
     2461 ggatgtaatt ttcgagtaaa tttatttgcg tagcctaatg ctgtctcttt attgtgacgt
     2521 aatttttcca acgctggata tggacgttgc ttaagttgac cattttctac atttaataca
     2581 cgaggaattg ttaagcaatg ggcccatcct tcattatctg taggatattc gatttccggt
     2641 aaccccatcc atccaattag tactcgtcgg cctttttcat ctaaaaatgt ttgaggtgca
     2701 tagaaatcaa aaccattatc aagctcgaca aaattttcat gttcatatgt taacttttca
     2761 atatcaaact ttccaagtat ataaccactt tgataaatat tgttgaactg atcgcatttt
     2821 ggttcaatac cttgtggaca aataagtatg acatcttgat tatctacatt aaagtaatca
     2881 gggcattccc acatatatcc aaaatcatct aactctgtat ttatttcacc taaataatgc
     2941 caattaatta tatcttcagt attataaagt aataatcgac cttgctgatc attattttgt
     3001 gcgccaatga tcgcataata tttctcacca tatttaaaaa ctttaggatc tctaaaatga
     3061 ctcgtatatc cttctggttg ctggctaatt actggctttg gaaacttttc aactgaaccg
     3121 tcttctttca atcgtgcgat catctgactc gcatggcgtt gccaatgatt atctcgatga
     3181 tttcctgtgt acatataata taaatgcccg ttatattcaa aagcgctacc gctatataca
     3241 ccatggctgt catatttggt atctgggttt aaaattggcc cttcagcttt aaagtttatt
     3301 aagtcatcac tcgtgtagtt ataccaatac tttaagccat gtactgcgcc taatgggaac
     3361 cattgatgtg aaacataata ctttccttta taaaaaataa gtccgttggg atcgtttaat
     3421 aagcccgttt ctggttgtat atgaaattgt tgacgaaatt ttgattgatc aacttgttgt
     3481 tttaatgttt taaaataatc agtatcaacg tcctcgattc gttgataacg ttcttctcta
     3541 gtccattcgg tcataatatt cacccagtcc tccttttata atttatgctg ttaacaattt
     3601 atattctatt atagcaaatt ttacctacgt tctttaactt ttaacctatc catttatact
     3661 tttatggtat cggttccaca tttattttaa aaaatacagc gtctaaatat taacatctac
     3721 tgtgacgcta tatggcatat cttgcttttt aagcatctgt tgtatctctt ccatcgcgca
     3781 ttgaccagct tcaaaataat tataatgaat tgtttttatc gatggagaca ctaattgtgt
     3841 cattggatca ccaccaaaac catatatttg atgtggtttc ataacatctt ttttatcaga
     3901 ataatattta taggcagcta atgcaatcgt atcagttgct ccaacaaccg catctacttg
     3961 ctctacattt tccaaaacat ttgcaacatc tttttgtgct tccacataag taaaatttat
     4021 ttcatgtata ttaggtttaa tttgatattt ggctaactgg tcaagtaaac cacgttttct
     4081 atgtatacca actgcaatat ctttttcact cacactaaac acttcaactt gttgatatcc
     4141 ctgttgacca atccattcac ctataatttg acctgcttta taatcatcat gcacaatact
     4201 atgcagttgt tcatgttgtt gaccaacaat aacgattggt acattcattt tattaatgac
     4261 ttcaatatgt ctctctgtta tgtctgtagc cattaaaaca ataccatcta ctttactgcg
     4321 tgctaatgtt tcaagcgctt gtatttctgc ttcgatattt aaacctgtat aatttaaaat
     4381 taattgtgat tcatattttt ggcattgttt tgccaatcct ttgattgttt catctactgc
     4441 atatgaattc attctaggta taatggcacc aataaggtgt gtttgtctcg ctcttaagct
     4501 ttgagcaaat tgattcggtt gatagtcatg ttctgctata atttttgtta atttttcact
     4561 tgttttttta ctgacagatc cattatttaa aaatctagat actgtacttt ttgaaacgcc
     4621 tgccaatttg gcaatatcag atatattttt catacttatt cacctatcat tatttgtgac
     4681 acttagcctt attttagcat atgtgtaacc gctttaaata atatttctgc ttctttcata
     4741 aaaagatttc aaaataaaat ttctgtataa caaaatgtcc caacatcaac ctttagttaa
     4801 atgtcaggac aatgaatatt ctatgtatat ataaatctaa tatcgaatat gtttattgaa
     4861 tctatttaac tcaccttcaa agtaagcatg ttcaccatga acaatcttgt ctaaccctat
     4921 attcgtttct tgttctgtaa cagataatgg tgtaattaat ttaattactt tcgcaataat
     4981 aaacgtcatg acgatgctaa aaattacaac tgctgttaca cataatattt gcacaagtat
     5041 aatatgcacg tcaccagtgt aaataaaacc attctcaata tctggatttg cttttttact
     5101 ttggaaaaag gctgttaata ctgcaccaat aataccgcca acaccgtgga taccaaatgc
     5161 atctaatgca tcatgatact ttaattttac ctttatgtaa ttgatgacaa tataacaaca
     5221 gataccccct attaaagcca tgattgttgc actaagatat gttacatatc cagctgcagg
     5281 tgtaataaca actaatcctg ctaatgcacc gagtaaaagt ccaagtaaac ttgtcgtctt
     5341 tttaaaaata tattctaaaa ttaaccaacc aatagccccg gcactggcag aaataactgt
     5401 attcgtaaat gcaagcattg caatttgatc aaatgtaaaa gcactgccta cattaaatcc
     5461 ataccaacca atccacacga atataccgcc aatcaatgta atgataagat tatgtggtgt
     5521 tgattcagaa tgtttgtttc cttttccaat cattatagct aatactaaac cagaaacacc
     5581 tgatgtaata tgaacgactg tacctccagc gaaatctaat acaccgagtt tgttaatcca
     5641 accgccgccc caaacccaat gtgctactgg actgtataca agagcagtcc atattactac
     5701 aaataataaa taaggaataa acttcatttt ctcagcgatt gaacctgata atattgagat
     5761 tgcaatcgta caaaacatca tttggaataa cataaataat gccaatggaa tatgcggact
     5821 aatatcttct tgtgtcgcga aacctacatg attaagaaat gtatattccc agttaccaaa
     5881 ccataaattc ccttccccaa aactaattgt aaaacctact gttatccata caaatgtaac
     5941 aagcacaatt gctgccatac tttgcatgac agtatttagc gcatttttag attgaactaa
     6001 cccaccataa aataaactta atcctggtgt cattagccaa actaataatg tacacaaaaa
     6061 cataaatatc gtatcgttaa gattcatact taccactccc ttgtttccat aataatctga
     6121 taatttataa aaagcaaaaa tgcgtaaagt tttattacat cttttggaac aaaatgtgcc
     6181 acgctatata aaaaatgatt tcatgaacta aaacaaaaaa gactacttac tcgcttttaa
     6241 agtagcggaa gtagccatag aatctaatat caattttatt attaaaatga taacgttaaa
     6301 ctttttgaac tgtaattgtc catgaagcat tatcaatttg ttcatagttt gttacaggat
     6361 atccattttc tgcagcccaa tttggaatag cttccgtcgc ttgtgtgcaa tcaaaatcaa
     6421 tttttaattc atctccagat tgcaatgttg ccattttctt ttgcgcttca attaacggaa
     6481 atggacatac cattcctact gtacctaatt cgtgtatcat agctatttct ccttttccaa
     6541 tcacttttat ttgatcaaca tcattggcat aataaccttc tatactattt gcgtttgctg
     6601 ttggaccttt gcagattgtt gttgtacttt tttcattgga cgaacaaaga taaaatgact
     6661 cattgtccat accccaacta tcatcgatgc tagcgcaatc catccttgcc aagtcatcgt
     6721 tgccgtttca accaaaccgt taccgataga acaaccacca gcaactgacg caccaaatcc
     6781 catacatata ccaccaatag cactgtttcg tattgtaatc ttgtctggca atcgccattt
     6841 aaattctctt gctcctctag ctgcaatata tgaaccaatg aaaattccta gaactaaaaa
     6901 gacaccccaa tcaataaatt tagtttcacc tgtaatcaaa aagtgtacta aatttgctga
     6961 tggcgttgtt atacctaaac cgtcatttct tccagttgat gcactcattg gccaagctaa
     7021 gagtgcgata agtccaattg caattgctgc aataaatgga tggtatcgtt tttcgaaaag
     7081 gtaatatcta atacctgtat aacgttgctt tagttttgga acagcaactc gaactttctt
     7141 attattgagc gttcttacaa cgagaaaaat ggtgattata gttaatataa cgactaatcc
     7201 ccaaaacgga atgcccgttg tttgagacat atcactattt acattcgttg gttgattaat
     7261 tttatccatt actggcttta aaatccctgt tttagtgatt gctgcagtaa cagcatataa
     7321 tactaatgca atccaactac caattagccc ttcaccagcg cgataccaag tacctgttgc
     7381 acatcctcca gccaatacta ttccaattcc aaaaataaaa gaacctataa ctgttcccaa
     7441 tattggaaaa ctatgtgcag gaatttgtaa aatacctgtt gtcgtcaaaa tcaataatcc
     7501 tatactttga acagtaatag cgattaataa tgcatagaac atcttattat tcttttgcac
     7561 atacatatct cgaaagccac ctgttaaaca aaatcttgtt ctttgcatca caaaacctaa
     7621 gagtcccccg acaattaagc cactaaaaat catccacata atcctaaccc ccctaatttc
     7681 cgataagttt agtatgtatt ataggcgatg attttcttgt tttcaacctt atacacaata
     7741 tttttgatat ttatattatg ttcatataag aaatgcgatt aattacgatt catcattata
     7801 tctctacgtg ttaatttatt aacctatatt atatttatca acacaacagt tttccctttc
     7861 attttcaaac ataacagaaa aagcctgaga catacttagt cccagactgt tctataatta
     7921 attaaagata taacaaggtt aatcattcaa agctaacatc acatttattc actgtaattt
     7981 ttcataaaga ataataatga ctgtaattct atacctaagt caatttgatg tacttgcaca
     8041 tctgaaggcg tatttattct accaggagtg aagtttaaaa tacctttcac accagcttgg
     8101 acgagttcat ctgcaacttt ctgtgcaact ctttctggtg tagttagaat cacaacatct
     8161 atttcttcct tcttcaatgt tgttattaat tcatcgttat cttttacaat aacgttccct
     8221 attttctggc caataacatc ttcttttacg tcaaacgctt ctgtaatcgt catatcgtca
     8281 tgtattgaaa agttatatgt gagcaaagct ttccctaggt tcccaactcc gacaattgcg
     8341 attttgatca tgtcactctc gcttagttca gatttaaaga aatccaataa actatctata
     8401 ttatatccgt accctttttt acctaattcg ccaaaatatg aaaagtcacg acgaattgtt
     8461 gccgagtcaa tttgtaacgc atcgctaatc gcttttgaat ttacacgatc tataccttta
     8521 gattttaatg aactgacaaa tctataatat aacggcaaac gttttaaagt tgctcgagga
     8581 attttaactt ggtcactcat tcgctatttc ctccttcgtg tttgaatgaa ttatatctat
     8641 gtattcaaac gagttagagc gtaattgtta gattattaaa aaacaaataa ttgattacaa
     8701 tgaataccta aatattatac attattgaat tttaaaaata aatatcttag tttcaagacg
     8761 gcgttttata ctgtaaaatg gttattaatg taataattac gacttttatc ataagttaat
     8821 aaaatttgcg aaagacgaaa ggtgaagatc gaatgatact tttacaactc aatcatatat
     8881 caaaatcgtt cgatggtgaa gatatattta atgatgttga ttttgaagta aaaactggtg
     8941 aacgaattgg tatcgtagga agaaatggcg ccggtaaatc aacattgatg aaaattatag
     9001 ctggcgtaga aaactatgat tctggaaatt tgtcaaaaat taaaaactta aaactaggtt
     9061 atttaactca acaaatgact ttaaattcta atgcaacagt tttcgaagaa atgtcaaaac
     9121 catttgaaca tattaaacga atggaaagct taatcaaaga agaaacagat tggttatcaa
     9181 aacatgcaaa tgattacgat agtgatacat ataaaacaca tatgtcccgt tatgaatctt
     9241 tatcgaatca atttgaacaa ttagaaggat atcaatatga aagtaaaatt aaaacagtac
     9301 ttcacgggtt gaattttagt gaagaagatt tcaataaacc tatcaatgat tttagcggtg
     9361 gccaaaaaac acgtttatct ttagctcaaa tgctattaaa tgaacctgat ttattacttt
     9421 tagatgaacc tactaaccac ttagatttag aaacgacaaa gtggcttgaa gattatctac
     9481 gttattttaa aggtgcaata gtcatcatca gccatgatcg ttacttttta gataaaatag
     9541 ttactcaaat ttatgatgtg gctttaggtg atgtcaaacg ctatgttggt aattacgagg
     9601 aatttataca gcaacgggat ttatattatc aaaaacgaat gcaagaatat gaaagtcaac
     9661 aagcagaaat aaaacgatta gaaacttttg ttgagaaaaa tattacccgt gcttcaacaa
     9721 gcggaatggc aaaaagtaga cgtaagattt tagaaaaaat ggaacgcatt gataaaccaa
     9781 tgttagatgc caaaagtgca aatattcaat ttggctttga ccggaataca ggtaatgacg
     9841 tcatgcatgt aaaaaattta gaaatcggtt atcaaactgc aattaccaaa cctatcagta
     9901 tagaggtctc taaaggcgat catatagcaa tcattgggcc aaatggtatt ggaaaatcga
     9961 ccttaattaa aactattgca aatcaacaaa aagcgcttaa tggcgatatt acttttggcg
    10021 caaatttaca aattggttat tatgatcaaa agcaagcaga atttaaatct agtaaaacaa
    10081 ttttagatta tgtgtgggat caatatccgt taatgaatga aaaagatatt cgtgcagttc
    10141 ttggacgttt cttatttgta caagacgatg ttaaaaagat aattaatgat ttatctggtg
    10201 gtgaaaaagc acgtttacaa ctagcactac ttatgttgca acgtgacaat gtacttattt
    10261 tagatgaacc taccaaccac ctcgatatag attcaaaaga aatgttagag catgcactcc
    10321 aacattttga aggaacaata ctatttgttt cccatgatcg ttactttatt aaccaattag
    10381 caaacaaggt atttgattta acaattgatg gcggaaagat gtatttagga gattatcaat
    10441 attacattga aaaagttgag gaagcggaag cgttgaaagc gcaccaagaa gaacagtcag
    10501 ttaacgtaca agcacatgaa aagtccatgg aacaatcgtc gtatcataac caaaaagaac
    10561 aaagacgtga acaacgcaaa cttgaacgac aaatatccga gtgtgaaaat gaaatagaaa
    10621 ctttagagac tactatctta caaatagacg agcaattaac ccaaccagaa gtttataaca
    10681 atccacaaaa agcaaacgta ttagctatcc aaaaacaaga tagcgaacaa aaattagaac
    10741 acgctatgtc taaatgggaa gaattacaac aaaaactata aaaatataca taactctaac
    10801 tgtaaaatcg ctatagttag agttattttt ttacaaattt taaatttttt tattcacaat
    10861 aaaaacgctg atattatagc tatccacaga gttatcctag ttatccacaa gtaaattaac
    10921 actaatcaat catttatttt ctgttttccc acctgatatc cacaataaat gacgttatca
    10981 acacctttta tccacaagtt atacactctt tgttgataag tacgcatgtt ctctattgac
    11041 attgttttta tcaaaatcca ttgttaagac attttcttta tgaaataata ataatattat
    11101 tattgaagaa tatttttatt attattaaaa ataaataagg agatagttat gagcacaaat
    11161 caaacatttt taatatttgt tatagcaatt attatactta catcggtaat aggaattgtt
    11221 ggacgataca tgagtcgtca acgtctatta aaatctatgg aaacattatg gcaaacgatt
    11281 tctccattag agtcttttat cagaccgaac tcacatttcg actatgagta taagctctac
    11341 aaggataaat ttgaatcaca ttctttagtt gatgataaaa cttggtccga tttaaatatg
    11401 aatgcaatct ttcataagat gaattataat ttaacagcta ttggcgaaat gaaactatat
    11461 gcctgtttac gtggaatgct ttcaattacg aacaaatcat tacttagttt atttaatgat
    11521 aatgctgaat ttagaaaaaa cgtaacatat catttagctt tgattggtaa aactgtttat
    11581 ccaacatttc cagaccaaat tacaccggta aaacgtcata atatattgtt tctatgcccg
    11641 tttttaccag ttatctcatt tgcagttata tttattaatt cacaagttgg tattttatta
    11701 tttcttatga gttgcctatt taatatcatt ttatctgcaa ctttaaagcg tacatatgag
    11761 gatgacttaa agtctatatt ttatgcatcc aatgttttaa aacaaggcta tactatctca
    11821 aaaattaaac atgctccaca acccgaagtc aattttaagc aatttagaac agcacgtcat
    11881 ttaacgagtg ttttagctga agttaatgat gaagatatcg gagctatggt aattaaacta
    11941 gttaaactta ttttcatgct tgattatgtt ttattccatt caattcaaaa aagctataca
    12001 acacatataa atgagctaaa aaattgtttc gattacatag ctgaattaga taatcattat
    12061 gcattagcaa tgtatcgtag aacgctagaa tgctataccg aaccacaaat tgatgattca
    12121 aatgatggca tagtattctc cgagttaaca catccactca tagcagatgc agtagccaat
    12181 gatttctcac tatcacaaaa tatactgtta actggatcta atgcttcggg taaatcaaca
    12241 tttatgaaat ccatagcaat aaacatcatt cttgcatcgg ctatacaaac agttaccgca
    12301 agcaaatttg tatatcaacc tggaattgtg ttcacgtcaa tggcaaatgc cgatgatgtg
    12361 ttatcaggcg atagttattt tatggctgaa ttaaaatcta ttaagcgtat tgttgaaatt
    12421 cctgataatc aaaaaatata ctgctttatc gatgaaattt ttaaaggtac caacacaact
    12481 gaacgaattg ccgcttcaga atcagtacta tcatttttac atgaaaaatc taactttaga
    12541 gttattgcag caacacatga tattgagtta gctgaactct taaaacaacg ttatgaaaat
    12601 tatcatttca atgaggtaat agaaaataat aacatacatt ttgattacaa aattaagcct
    12661 ggcaaagcaa atacacgtaa tgccatcgaa ttattaaaaa tcacttcatt tccagcaaaa
    12721 atatatgaac gagcaaaaga taatgtttcc aatggttagc atgaatcttg aacatcaatc
    12781 aatgttcaag tcgcaacatt gttatattgg cagtctttga ccaaatgaaa atgcgcttgt
    12841 aacaagtttt ttcaatacta gtccgcggcc ccaacacaga agctggcgga aagtcagctt
    12901 tcaatcgtgt acaagttggc ggggccccaa cataataaat cattagtggt tctttatctt
    12961 ttctgtctca ctcccctaat tgcaatataa aaacgtcagc tatcacatga cagaagacaa
    13021 tgaacagttt caataatatt catcgtaatc atgttaataa ctgacgttta ttttattctg
    13081 cagaatactc ttctaaatct atattgctgt gcccatttaa tgctaaatca gcaaatcgac
    13141 cttgctgata caaatagtgg ccggcaacgc ctatcattgc agcattatct gtgcataatt
    13201 taggacttgg gatagttaat tgaatgtcat tgactttgca ttgatccgct aaagattgac
    13261 gtaatccttt attactcgcc acgccaccag caacaattaa tcgctgaaca ccatattctt
    13321 tacaagcttg aatagcttta aacgtgagaa cctctacaac actgttttga aagctcgttg
    13381 ctacgttagc ttcaatgatt ggaatatttt tttgtcgttg attgtgaagt tgattgatta
    13441 cggcactttt caacccacta aaactaaaat cataactatc tttatccaac caaacacgag
    13501 ggaatgaata agtatcttca ccttcagcag ccaaccggtc aacttgtgga ccacctggat
    13561 aatttaaacc aattgttcgt gccactttat cataagcctc acctactgcg tcatctcgtg
    13621 tttcaccaat gacttcaaat gttaaatgat ctttcatata aactaattca gtatgtccac
    13681 ctgaaacaat aagtgcaatt agcgggaatg ttaatggctc ttctatgtga ttagcatata
    13741 tatgtcctgc aatatgatga acaggaataa gcggcttatc gtaagcaaat gccaatgctt
    13801 tggctgcatt aacacctatt agtaacgcac caattagtcc agggccttct gtaaccgcta
    13861 tggcatcaat atcttccatt gatacgtcgg catccactag agcctcgttt attgttgttg
    13921 ttataccttc aacgtgatgt ctacttgcca cttcgggaac gacaccgcca aatcgtttat
    13981 gactttcaat ctgacttaaa actgtatttg ataaaatatc tctgccattt tttataacac
    14041 taacgcttgt ttcatcacaa cttgtttcaa cagctagtat taatatatct ttagtcattt
    14101 aaattcaccc acataaccat tgcgtcctca ccttcaccat aataattttt acgtttacca
    14161 ccatattgaa atcctaaatt ttcatataca tgttgtgcca ctttattatt aactcttact
    14221 tctaaactca tcacatcaca agtgtgactt gcatagttta ttccgtattt taaaagcatt
    14281 tgacctaaac catatcctct ataattatca tcgattgcaa ctgtcgtaat ttgagcttga
    14341 tcgataacaa tccataaacc taaataacca ataatttgtt gttcaaattc taagacaaaa
    14401 tatttcgcaa agttattttg ctctatttca tgataaaatg cgtcaattgt ccaagaacta
    14461 tcattgaaac tccgacgctc aatatcaaag acttgtggca catcttcttt ggtcatctct
    14521 ctaatgttta attgttcttt tgactgttga tccaatttcg ttccgcctca gctaatttat
    14581 ggtatttagg agtaaatgta tgtacgtctg aaggtttatc tatcaattga tacatgactg
    14641 atgcatttgg tagctgcgca atcacatcac cttgtaaatc atcttgtaat tttactgtat
    14701 ctttcccaat ataaataaat ggttggttta aatcttctaa aaaagctcgc aatgcatcta
    14761 tcgacatata ttgatcttct aaaatagtca ctaattgacc attttgccat tggaatatgc
    14821 ctgtataaac tgcttgtcgt cttgcatcaa acacaggaac caataattta tcagtatgat
    14881 cgattgttgc tgccaatgcc tttaatgatg aaacaccata taatttaaca tctaacgcat
    14941 acgctaatgt tttagcaaca gtaacaccga tacgtaagcc agtatatgaa ccaggacctt
    15001 cagcaacaat aatcgcatct aattgctgtt tagctatttt gctctgctca aatagctgac
    15061 taatcgcagg cattaattgc actgaatgat tttgtttaga atccgtagtt atttcagcta
    15121 aaacttcatc gttttgcatc aatgctactg ataatggttg attcgatgta tcaatgagca
    15181 gcgaattcat ggataattgc ctccttaatt tgttcataat gttctccttg cgcgaacaac
    15241 tcaatttgtc ttgaattttc agatattgtt gaaatgttaa tagataaatg cgtcgctggg
    15301 agtaaatctt ttataaattg actccattca ataacagtaa ttgcctgatc ttcgaaaaat
    15361 tcatcaaacc ctaaatcttc atcagaatct tctaagcgat aacaatccat atgatgcaat
    15421 tttaaatttt ttcccctata tgatttgatg atgttaaatg tcggggaatt aatcgtacgt
    15481 cttacaccaa gagcttttcc tataaattgt gttaacgttg ttttacctgc tcctaaatct
    15541 ccgttaagta aaatcaaatc accacttttc aattgctcaa ctaaaaacat agcaaattga
    15601 ttcatttcat ctaaattatt tatctttatc aatgttgatt ctcctatatt atgcttttca
    15661 ttcataaaaa tgattatcca ttgttcaatc gtatctaact ttatatttaa cctttatatt
    15721 gtaacaaatt tcaacttaaa tttcttatct ttgaaacaga ttatctcttc aaagttaatt
    15781 gtaagaaaat ttaaaatatt tgttgacata ctaaagcaga tatagtaaat taaatttatc
    15841 aaatttttag acaattctaa ctattaaagt ggtatatacc attcacggaa ggagtataat
    15901 aaaatgctta atcaatatac tgaacatcaa ccgacaactt caaatattat tattttatta
    15961 tactctttag gactcgaacg ttagtaaata tttactaaac gctttaagtc ctatttctgt
    16021 ttgaatggga cttgtaaacg tcccaataat attgggacgt ttttttatgt tttatctttc
    16081 aattacttat ttttattact ataaaacatg attaatcatt aaaatttacg ggggaattta
    16141 ctatgcgaag cgacatgatc aaaaaaggag atcaccaagc accagcaaga agtct
//
LOCUS       DAMZPP010000062        15916 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMZPP010000062 DAMZPP010000000
VERSION     DAMZPP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15916
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.062"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..317)
                     /locus_tag="POH73_001464"
     CDS             complement(<1..317)
                     /locus_tag="POH73_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDI6887422.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     CFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKVELIDQVGYFIEPQDLFSAMIH
                     EIET"
     gene            complement(481..615)
                     /locus_tag="POH73_001465"
                     /pseudo
     CDS             complement(481..615)
                     /locus_tag="POH73_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(680..1399)
                     /gene="splF"
                     /locus_tag="POH73_001466"
     CDS             complement(680..1399)
                     /gene="splF"
                     /locus_tag="POH73_001466"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HDI6887423.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTMVEGIQQTAKAENSVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKYEAIGVIYAGNKPSGEST
                     RAFAVYFSPEIKKFIADNLDK"
     gene            1503..1620
                     /locus_tag="POH73_001467"
                     /pseudo
     CDS             1503..1620
                     /locus_tag="POH73_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1569..2285)
                     /gene="splE"
                     /locus_tag="POH73_001468"
     CDS             complement(1569..2285)
                     /gene="splE"
                     /locus_tag="POH73_001468"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HDI6887424.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTMVEGIQQTAKAEHNVKLIK
                     NTNVAPYNGIVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRYAGKEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNMIISDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPANKSTK
                     GYGVYFSPEIKKFIADNLDK"
     gene            complement(2449..3164)
                     /locus_tag="POH73_001469"
                     /pseudo
     CDS             complement(2449..3164)
                     /locus_tag="POH73_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038688.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
     gene            complement(3331..4047)
                     /locus_tag="POH73_001470"
     CDS             complement(3331..4047)
                     /locus_tag="POH73_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDI6887425.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVSTTMVEGIQQTAKAEHNVTKIT
                     NTSISPYKGILRVGAATGFVVGKNTILTNRHVVKDVQVGSTVLAHPNGENDTGGYYKV
                     KKVIPYAGSADLAIVEVEEDSVYPKNKKFSENTEILTLASEAKANERVAIVGYPAPYK
                     NKHHMYQSTGTVLSINGDKLVSDAFAEGGNSGSPVFNNKNEVVGVLYSGDQIGHSKKE
                     SYSVYLTPEIKKFIADNTEK"
     gene            complement(4171..4890)
                     /gene="splC"
                     /locus_tag="POH73_001471"
     CDS             complement(4171..4890)
                     /gene="splC"
                     /locus_tag="POH73_001471"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HDI6887426.1"
                     /translation="MNKNLVIKSMAALAILTSVTGISAAVVEETQQIAKAEKHVTQVK
                     DTNVFPYHGVVAFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGNEDISVMNIEEQAVERGSNGFNFNDNVQAFKFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKVGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            complement(4948..5079)
                     /locus_tag="POH73_001472"
                     /pseudo
     CDS             complement(4948..5079)
                     /locus_tag="POH73_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
     gene            5905..6420
                     /locus_tag="POH73_001473"
     CDS             5905..6420
                     /locus_tag="POH73_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887427.1"
                     /translation="MIKQVLRKSLHTVKEHIDKSNFQKNLIFSLVIFPFLFVPYIIID
                     ILVHLSNNQEYFKLLSSISHIFLMFFVTAISLYAILSIPLTSNSCRKSSFRKISKVII
                     YFLVFFYILSHHINIDISKMDSSWHFYALFLCFFIISYKLTEKIMKVCKWIFNEESNN
                     DEGLRTQKLFL"
     gene            6667..8013
                     /locus_tag="POH73_001474"
     CDS             6667..8013
                     /locus_tag="POH73_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HDI6887428.1"
                     /translation="MKKEILKKQWVLWTILVTSFFSIGTPGIAIIPFSLSIYALSKLI
                     LVNKFVEPDLVTLQNLKEKNKSINIENQKIKREIQELKNLKKDLISSIEEGTKELEHI
                     TSYLNDELFKYDIELTYPFDLVEVDSSQINTYIKKLQMKEKELLNLEEVKIFNVSTEN
                     KRHQNAQAKQIIRLFNAETSQLINKVNSKNIESMQNKIFKSYEGINKIFETDNVRIPE
                     TLLDIKLEMLDLMHKYQVKIEDEKIIRREERARLKEIEQAEKEMEKKLKELDKDIRHH
                     NNEIKKLTMYLNNTDLQVEKELYIEKIRELDQSLKNLNSERENVEDRKDNAQSGFVYI
                     ISNIGSFGENVYKIGVTRRLEPMDRINELSSASVPFEFDVHALIFSENAFELKNKLHE
                     YFKKYKVNKVNGRKEFFKVNINEIKDKVLSEHNSTVQFIDEPKAIQYRETLRLTSL"
     gene            8271..8499
                     /locus_tag="POH73_001475"
                     /pseudo
     CDS             8271..8499
                     /locus_tag="POH73_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121211.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8600..8836
                     /locus_tag="POH73_001476"
     CDS             8600..8836
                     /locus_tag="POH73_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887429.1"
                     /translation="MIRFDVNGSDHANSPNNERIPTPHIHIYTEEYNNGGIAIPLKDI
                     EDLELTDEIIESLDFFMKYTNIKHDNVIIEPRLL"
     gene            8857..9633
                     /locus_tag="POH73_001477"
                     /pseudo
     CDS             8857..9633
                     /locus_tag="POH73_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
     gene            complement(10208..10984)
                     /gene="seg"
                     /locus_tag="POH73_001478"
     CDS             complement(10208..10984)
                     /gene="seg"
                     /locus_tag="POH73_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type G"
                     /protein_id="HDI6887430.1"
                     /translation="MKKLSTVIIILILEIVFHNINYANSQPDPKIDELNKVSDYKSNK
                     GTMGNVMNLYMSPPVEGRGVINSRQFLSHDLIFPIEYKSYNEVKTELENTELANNYKG
                     KKVDIFGVPYFYTCIIPKSEPDINQNFGGCCMYGGLTFNSSENERDKLITVQVTIDNR
                     QSLGFTITTNKNMVTIQELDYKARHWLTKEKKLYEFDGSAFESGYIKFTEKNNTSFWF
                     DLFPKKELVPFVPYKFLNIYGDNKVVDSKSIKMEVFLNTH"
     gene            complement(11268..12023)
                     /gene="sen"
                     /locus_tag="POH73_001479"
     CDS             complement(11268..12023)
                     /gene="sen"
                     /locus_tag="POH73_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type N"
                     /protein_id="HDI6887431.1"
                     /translation="MRLFCIAAIIITLLCLINNNYVNADVDKNDLKKKSDIDSSKLFN
                     LTSYYTDITWQLDESNKISTDQLLNNTIILKDIDISVLKTSSLKVEFNSSDLANQFKG
                     KNIDIYGLYYGNKCVGLTEEKTSCLYGGVTIYDGNQLDEERVIGVNVFKDGIQQEGFV
                     IKTKKAKVTVQELDTKVRFKLENLYKIYNKDTGNIQKGCIFFHSNNHQNQSFYYDLYN
                     IKGSVGAEFFQFYSDNRTVSSSNYHIDVFLYKD"
     gene            complement(12062..12847)
                     /gene="seu"
                     /locus_tag="POH73_001480"
     CDS             complement(12062..12847)
                     /gene="seu"
                     /locus_tag="POH73_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type C1/U"
                     /protein_id="HDI6887432.1"
                     /translation="MKLFAFIFICVKSCSLLFMLNGNPKPEQLNKASEFTGLMDNMRY
                     LYDDKHVSEINIKAQEKFLQHDLLFKINGSKIDGSKILKTEFNNNSLSDKYKNKNIDL
                     FGTNYYYQCYFSADNMELNDGRLIEKTCMYGGVTEHDGNQIDKNNSTDNSHNILIKVF
                     ENERNSLSFDIPTNKKNITAQEIDYKVRNYLLKHKNLYEFNSSPYETGYIKFIEGNGH
                     SFWYDMMPESGEKFYPTKYLLIYNDNKTVESKSINVEVHLTKK"
     gene            complement(13001..13729)
                     /gene="sei"
                     /locus_tag="POH73_001481"
     CDS             complement(13001..13729)
                     /gene="sei"
                     /locus_tag="POH73_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type I"
                     /protein_id="HDI6887433.1"
                     /translation="MKKIKYSFILVFILFFNIKDLSYAQGDIGVGNLRNFYTKYDYID
                     LKGVTDKNLPIANQLEFSTGTNDLISESNNWDEISKFKGKKMDIFGIDYNGPCKTKYM
                     YGGATLSGQYLNSARKIPINLWINGKHKTISTDKIATNKKLVTAQEIDVKLRRYLQEE
                     YNIYGHNNNGKGKEYGYKSKFYSGFNKGKVLFHLNDEKSFSYDLFYTGDGLPVSFLKI
                     YEDNKIIESEKFHLDVEISYVDSN"
     gene            complement(13764..14483)
                     /gene="sem"
                     /locus_tag="POH73_001482"
     CDS             complement(13764..14483)
                     /gene="sem"
                     /locus_tag="POH73_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_058008129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type M"
                     /protein_id="HDI6887434.1"
                     /translation="MKRILIIVVLLFCYSQNHIATADVGVLNLRNYYGSYPIEDHQNI
                     NPDNNRLSHQLVFSKDNSTVTAEFKNVEDVKKFKNRAVDVYGLSYSGYCLKNKYMYGG
                     VTLAGDYLEKSRCIPINLWVNGNLKTISTDKVSTNKKIVTAQEIDTKLRRYLQEEYNI
                     YGFNDTNKGRNYGTKSKFFSGFNTGKISFHLNDGTSFSYDLFDTGTGQAESFLKIYND
                     NKTVETDKFHLDVEISYKDES"
     gene            complement(14765..15547)
                     /locus_tag="POH73_001483"
     CDS             complement(14765..15547)
                     /locus_tag="POH73_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HDI6887435.1"
                     /translation="MKKSKAMLNVLLLIINLIAICSVNNAYANEENPKIEDLCKKSSV
                     DDIALHNIDKDYMTNRFTINESTVLTTEKFLDIDLLFKNFTWLDGKSAEFKDLKVEFS
                     SSEISKEYFGKTVDIYGVYYKAHCHGEHQVKTACTYGGVTPHENNKLSEPKEIGVAVY
                     KDNVNVNTFIVTTDKKKVTAQELDIKVRTKLNNVYKLYDRMTSDVQKGYIKFHSHSEH
                     KESFYYDLFYIKGNLPDQYLQIYNDNKTIDSSDYHIDVYLFT"
     gene            complement(15776..15864)
                     /locus_tag="POH73_001484"
     tRNA            complement(15776..15864)
                     /locus_tag="POH73_001484"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15827..15829),aa:Ser,seq:gga)
BASE COUNT         5505 a         2445 c         2068 g         5898 t
ORIGIN      
        1 gtttcaattt catgaatcat cgcgctgaat aaatcttgtg gctcaatgaa gtaaccgact
       61 tggtcaatta attctacttt taagtcttca cggtattctt catctgccca tgcttcttga
      121 tacgtgatgt cttcacctga caaggcatct gcatattctt gttcggcttt ttcagataag
      181 aagcaataga aaatcaagcc taaaatgtaa ttacggaatt cactcgcatc catgttccct
      241 cttaaatcat tcgcaatcga ccataatttt ttatgtaatt cagcttgttg ctgacgttgt
      301 ttttcagtaa tagacatgtg attcctccgc ctttgcataa gtaatttatc tctttgtgta
      361 atagatttat tataacattt ggttttgttg cgatgtttag aattttgatg ttggttgtgg
      421 aaattttgag ttttagcggc gcaattgtta ttgaaaattt ttttggaaat gttgtagctt
      481 tcaaatgctt tcaaaatcat ttatattctc aatgatgtca aaaagttgtt cattcataca
      541 taaataaaac caatcaacaa ttgagttggt gaaaatcaat cattgattgg ctttgatgca
      601 tattaaataa tgcaatatat attaataaat gttagttata gtatattttg tcaggattgg
      661 gtgaatgtct aagttttaat tatttatcta aattatctgc aatgaatttc ttaatttcag
      721 gagagaaata aacagcgaat gctcttgtgc tttcacctga tggcttatta cctgcataga
      781 ttacaccaat agcttcgtac ttactattta atataggtga tccagagcta ccaggctgga
      841 ttattgcatc cgatgacact atattcccat tcactgataa tactttacca gttgattcat
      901 acatttgtag tttatttcca ttaggatttg gataaccaat gactgatata ggttcatttt
      961 ctttagcctc tgatgctata ttaaatttgc tagtgaaatc tttgaatttt ctaccttttg
     1021 gttgtgttga tttttcttca acttgcacaa ccgcaatatc ttctttacca ggataatcta
     1081 caatcttagt aactttataa agtccaccac cattattata aaaaccatta ggatgtgctt
     1141 taatttcatc accgactttc atgtgatagg taacatgttt attagtaatg attgtatgat
     1201 ttccaactac aaatcctgtt cctgcgccca tccatgtaac cccactgtat ggtgcaacat
     1261 ttgtatttgt aatttgtttt acactatttt cggctttggc tgtttgttga ataccctcaa
     1321 ccattgttgt gccaacacca gtcactgatg ttaaaatcgt caatgctgca atacttttga
     1381 tgattatatt tttattcatt tttgtgctcc tataattatt cgtattaata taaggttagg
     1441 tttttgaagt tttatttttt taatcatttc gaataaatag aatcacggag atatgatttg
     1501 aattgttgat tttgttgtta atttttaaaa atcatctatc ttatgtaagg attgatttat
     1561 ctgcttaatt atttatctaa attatctgcg atgaatttct taatttcagg agagaaataa
     1621 acgccatatc cttttgtact tttatttgca gggccgtttc caccaaagtg aacacctaca
     1681 acttcgtatt tactattaaa tacagctgaa cctgagttgc ctggttctac gaaagcatca
     1741 gaaataatca tgttgccttt cactgatagc acttttcctg ttgattcata catttgaaat
     1801 ttatttatat aaggttctgg atagccaaca attgaaatgc gttcattttc tttggcttct
     1861 gatgctattt ttaaaatacc tgtataatct ttaaaattcc tattttttgg atgaacagct
     1921 ttatcttcca catgtagaat ggcaatatct tctttacctg cataacggac aattttttta
     1981 actttataaa aaccgccatt attatattca ccattaggat gcgcaataat atgtgcgcca
     2041 atttccatac ctgcaacgac atgcttgtta gtaacaattg tattttttcc gacaatgaaa
     2101 cctgttccag atcctatcga aacgatacca ttgtatggtg ctacattagt atttttgatt
     2161 agtttcacat tatgttcggc tttggctgtt tgttgaatac cttcaaccat tgttgtgcca
     2221 acaccagtca ctgatgttaa aatcgtcaat gctgctatac ttttgatgat tatattttta
     2281 ttcatttttg tgctcctcta agtattctta taattataat gttaggtttt tgagtttttt
     2341 tgaatcattt cgaataaata gaatcacaga gatatgattt gaattgttga gtttgttgtt
     2401 tattttaaaa aattatttat caatgtaagg atggatttgt atgttcgttt atttttctgt
     2461 gttatctgca atgaatttct tgatctcggg tgtgaaataa actaaatatg atttttttgt
     2521 tgcatcattt ttgacatcga cagcatatgc aattgcaatg acctcatgat ttgaattaaa
     2581 cactggggat cctgaattgc caccttcagc aaatgcatct gaagtcattt gattaccatc
     2641 gatggataat atctttcctg ttgattcaaa catatgatgc ttatttttat atggattagg
     2701 gtaaccaacg attgataaac gatcatttac ttgagcgcca ctcgcaaatt taaggacttc
     2761 agtattttca ctgaattttt tattttttgg ataaacagaa tcttcttcaa cttgaactat
     2821 tgctaaatct tcagatcctg cgtatggaat gattttttta ctttatagta tccaccggta
     2881 tcattttcgc catttggatg agcaagaatt gtgctaccaa cttgaacacc aggtaataca
     2941 tgcttatttg ttaaaattgt attttttcct accacaaatc ctgtagctga tccaattcta
     3001 acaattcctt tatacggaga aatatttgtg ttttgtactt gcttcacatt atgttcggct
     3061 tttgctgttt gttgaatacc ttcaacaatg gttgtgccga cgccagtcac tgatgttaaa
     3121 atcgtcaatg ccgcaatact tttgatgatt atatttttat tcatttttgt gctcctctaa
     3181 gtattcgtat taatataagg ttaggttttt gaagttttat ttttaaaatt atttcgaata
     3241 aatagaatca cagagatatg atgatagttg aattagttat taagtttaaa aatgtaatct
     3301 aagttgaggc atgtaagtga gctttccggc ttatttttct gtgttatctg cgatgaattt
     3361 cttaatttca ggcgttaaat atacgctgta tgactctttt ttagaatgac caatttggtc
     3421 tccactatat aaaacgccca caacttcatt tttattgtta aagaccggtg accctgaatt
     3481 gccaccttca gcaaatgcat ctgatactaa tttatctcca ttaattgata gtactgttcc
     3541 tgttgattga tacatatgat gtttattttt atatggcgct ggatagccaa cgattgcaac
     3601 tctttcattc gctttagctt ccgatgccaa cgttaggatt tcagtatttt cactgaactt
     3661 tttatttttt gggtaaactg aatcttcttc aacttcaacg atagctaaat ctgccgagcc
     3721 tgcatatggg ataacttttt tgactttata gtatccgcca gtatcatttt cgccatttgg
     3781 atgggcaagc actgtactac ctacttgcac atctttaacg acatgtctat ttgttaaaat
     3841 agtgttttta cctactacaa atcctgtagc tgcgcctact cttagtatgc ctttgtatgg
     3901 cgaaatacta gtatttgtta tcttcgttac attatgttcg gctttggctg tttgttgaat
     3961 cccttcaacc attgttgtgc taacaccagt cactgatgtt aatatcgtca atgccgcaat
     4021 acttttgatg attatatttt tattcatttt tgtgctcctc tgtttattca tgatgctttt
     4081 gatatagacg tttgcttttt atgttatatt cgaccgatgt ttttcattaa aattattgaa
     4141 cacatgctca tggtgtatat ttaaatatgt ttattgttca atgtgctttt gaataaaatc
     4201 tttaatttga ggcgtaaaat atacagcacc attgtattca gaaccaactt ttccaatgcc
     4261 gccatataca acacctatga cttcattgtt agaatttaga acgggtgatc ctgaattacc
     4321 tggttcgata tatgcatcaa aatttaaaat attgtcttta attcgtttta tagttcctgt
     4381 agattcaaat tgtttaaaac tgttttgtgc aggtaacggg taaccgataa ctttaatttt
     4441 gtcatcaact ttagcatctt ttgcaaattt aaatgcttga acattatcat taaaattaaa
     4501 gccattggat ccacgttcaa ccgcttgttc ttctatattc atgacggaaa tatcttcatt
     4561 gcctggataa tcggaaatac ttttaatttt ataaatacca ccatttcctt tgtcaccatt
     4621 tggatgagca gtaattctat cgccaacttt ataatccttt gatacatgtt tattagtgat
     4681 aattgtattt ttaccgatta caaagcctgt cgcatcttta aatgcaacga cgccatgata
     4741 cggaaaaaca tttgtatctt taacttgcgt aacatgcttc tctgcttttg ctatttgttg
     4801 tgtctcttca acgactgcag cacttattcc agttactgat gttaaaatgg ctaatgctgc
     4861 catgctttta atgactaaat ttttattcat tttcaagctc ctttacaatt tgagatatat
     4921 tgcaacgctc gttttaaaac aatttgatta tttatctata ttttctgcaa tgaatttttt
     4981 aatctctggt gtgaagtaga cgccataagc atttctattg tcatcatctt tcacatctga
     5041 agcaaaatga atacctacta gttcgttgtt gctatttagg ttgtgtatgc atgtcacaat
     5101 tctccctatc atgactatta ttccatataa ttatgatatt aattatcgac ttgtaaagtt
     5161 atattcttac ttgaaaagtt ataaacataa atgtcaaata atgctaatta tggtaagtta
     5221 atattacatt tagtaacatc aaagttaaca aatatagaag aaaatcctca actttctaat
     5281 tatgaatcag aatattatct tcttgaaaaa aatttaatta agacacaatt tatcaaaagt
     5341 actaaaagta gatgtctata tgaagaaacc acttatcaca tattgacgaa tggagacatc
     5401 ttaaaccaac ccggaaattt gaaattatgg acgtaggcaa ggcttttgtc caaccgtttc
     5461 attgcatgaa tttggaaaaa catgtcagat acctttatga aatttccgaa tatttgcatt
     5521 taagtaaatc ataaatttca gaagtattat gataatatat accattaaaa ccaattacta
     5581 attcattgtt ctaactatgc aggtaacttt ccgcaataag tccaaaataa atctaatgga
     5641 taaaaaataa tcaaaacttt aactttaatc aatattgaac tgcaaaaatt tggcacaagc
     5701 taaaacaatg attaaaccga cagggattgc caatgacaca ggtaaatcag tagaagaaat
     5761 gatgtgtaga agttcacaag taaactggaa aatagcaatt aattaaaatt cgtttcaacc
     5821 tttatacata ctaagcaaaa ttacacactt tataaaaata atattaattt tgtaaaattc
     5881 agcttatttg gaggattaaa agcaatgata aaacaggttc taagaaaaag tttacataca
     5941 gtaaaagaac atatcgataa aagtaacttc caaaaaaact taatattttc acttgtcata
     6001 ttcccattct tatttgtgcc ttacattata atagatatac tcgttcattt atctaataat
     6061 caagaatact ttaagttatt atcatctatt tcacatatat ttttaatgtt ctttgtaaca
     6121 gcaataagtt tatacgctat actttctata cctttaacat ctaattcatg taggaaatca
     6181 agctttagaa agattagtaa agtaataatt tattttttag tattttttta tatcctaagc
     6241 catcatatca atatagatat ttctaaaatg gactcttctt ggcattttta tgcgcttttt
     6301 ttatgttttt ttattatttc atataaatta actgaaaaaa taatgaaggt atgcaaatgg
     6361 atatttaatg aagaatcaaa taacgatgaa ggtttaagaa ctcaaaaatt atttttataa
     6421 cgactgtggt ttttggtaca ataagtatct tctcaacaat tgcaaacgtt ttagtagcaa
     6481 taaaacaatt tctaaattag ttatatcagc taaataataa aaggtagcaa aaattatata
     6541 gtaaaagcga tagtagatta ttaaaaaaat atatagcatt tgttctcaca gcgtcaaaac
     6601 tctaaagcaa ttaagccatc gagagtattt aaatacatat aaattcaaat taaggagaat
     6661 aggtaaatga aaaaagaaat tttaaaaaag caatgggtat tatggacaat cttagtaaca
     6721 tctttcttta gtataggcac accaggtata gcaataatcc cgttcagttt atcaatatat
     6781 gcattatcta aattaatact tgttaataaa tttgtagaac ccgatttagt aacacttcaa
     6841 aacttgaaag aaaaaaataa aagtatcaac attgaaaatc aaaaaatcaa gcgtgaaata
     6901 caagaattga agaatctaaa aaaagattta atatctagta tagaagaagg tactaaagaa
     6961 cttgaacaca ttactagtta tttaaatgat gaattattta aatatgatat agaattaact
     7021 tatcccttcg atttagttga agtagactca tctcaaatta atacatatat taaaaaatta
     7081 caaatgaaag aaaaagaatt actaaatctt gaagaagtga aaatatttaa tgtttctaca
     7141 gagaataaga gacatcaaaa tgcacaggcc aagcaaataa tacgattatt taatgctgaa
     7201 acttctcaat taataaataa agtaaatagc aaaaatatcg aaagcatgca gaacaaaata
     7261 tttaaaagtt atgaagggat aaataaaata tttgaaacgg ataatgtacg tattcctgaa
     7321 actttacttg acataaaact tgagatgtta gatttaatgc acaagtatca agttaagata
     7381 gaagatgaaa agatcataag aagagaagaa cgagctagac tgaaagaaat agaacaagca
     7441 gaaaaagaaa tggagaaaaa actaaaagag cttgataaag acattagaca ccacaataat
     7501 gaaatcaaaa agctgacaat gtatttaaat aacacagatt tacaagtcga aaaagaatta
     7561 tatattgaaa agattagaga attagatcaa tcactcaaga atttaaattc tgaaagagaa
     7621 aatgtagagg acagaaaaga taatgctcaa tcagggtttg tttatataat atcaaatatc
     7681 ggttcatttg gagaaaatgt ttataagatt ggtgtaaccc gtagattaga accaatggat
     7741 agaattaatg aattaagtag cgcatctgtt ccctttgaat tcgatgtgca cgctttaatt
     7801 ttctctgaaa atgcttttga gcttaaaaac aaattacacg aatactttaa aaaatataaa
     7861 gttaataaag ttaacggaag aaaagaattc tttaaggtta atattaatga aattaaagat
     7921 aaagttttat cagaacacaa tagcacagtt caattcattg atgaaccaaa agctatacaa
     7981 tacagagaaa ctttaagatt aacttcactc taaaaatttg ggtagcccgc ctacccttat
     8041 tatttttttc caacttttat gagtatgtca tatatcaata aaaaaacaac caaattgtaa
     8101 aaggttttat ggttttggat atgaatgtaa ttattgtaaa aagtaattat ttattaatca
     8161 atacattgta atattaagtg caacccaata aaaattcata aaaataccca caatgggcac
     8221 tttattgtaa ggtgtttaca accacgaaaa cacatttaag tattgccaat atgagcgata
     8281 actatcttat actaactgag cacgtataga gaaaattatt ctttaagacc tattgcacgt
     8341 aaattagacc gatccgcatc tactatctta cgtgaaatta gcagaaataa tgtaaataag
     8401 ttatatcaat ctgaaacggc tcaaaagaat tatgtaacta caagaaaact ttgtggtcat
     8461 cctactggat ttactccaga acttagcaat atcattaagt aaaatttaaa aataccacta
     8521 ctcttctgaa ttaatcgtga ttcgattttt taaaaccgat tttcttttat aaccaaggat
     8581 ttatgtttta accactgata tgattaggtt cgatgttaat ggtagcgatc atgcaaattc
     8641 accaaataat gaaagaatac ctacgccaca tatacacata tatactgaag aatataataa
     8701 tggtggtatt gcgatcccat taaaagatat tgaagattta gaattaacag atgaaattat
     8761 agaatcatta gacttcttta tgaagtatac taatataaaa cacgataatg ttataataga
     8821 accaagatta ctgtaaaaga aatggcggga tacttattga agactatcga agaaagaatg
     8881 aacgaatatt tcaactggtt aaaacaaaat tatatcttca aagaattaga tagttctaca
     8941 gaaattacta ctccctttaa gaaccatttg aatgatttta ttagaatata tgctgataca
     9001 ctacctaata atgaaatttg tctatcagat aacggattga cgattaatga attagaaatg
     9061 ttgggtattg atataaacac aaaaacgaga actaaactta tacagaatat tttaaatcaa
     9121 tttaatttaa aattagttga taaagaaatt acagcggatg ttaaaaatta gagttttgct
     9181 cagtctaaac ataatttaat tcaaggcata ttgaaaattt atgaccttac tttaactact
     9241 aaatctaacg ttacaaacag attctatgaa gaagtatttg aattcttata tgaccgagaa
     9301 ataagaggtt tagcacaagt ttctgtttct ggagaatcag gtttaaaata ttctattgat
     9361 tatattgttt cagaaacaaa atcacaacca gaaaaactag ttaactttac aaataactta
     9421 gattttaaca aaataactaa tgaagcttat atttaccgtg atatcaaacc aaacagacct
     9481 tccagaaaca aattagaacc tatcatgtta ataattgtaa atgatgtata tcatccgata
     9541 aatgaaagag cacaaactgt agcagaacat gagaaccttg agatattaaa atggtctaat
     9601 aaatcaaaaa tcattgaaac tttaacctca taaattaaca ttagacccct atttgtaatt
     9661 ctttaatttt caaatcatat cgtgcaagta aaaaagtaga tactttttta tttgcacgtt
     9721 ttattttaga acaaaaagag caaggtcaac gaatcagaaa atctttttct tgatacgtat
     9781 ttgacacttg ctcttttaag aatactaaca tttcattgta tttccgttaa attaacggag
     9841 aaggcggatt acctcgatat atttatctct agagtgttaa aaaattgtac attcttaaac
     9901 aatcaaaagt atacgttatt aaattatcat ttccactgga gaatcatggc gactaaaaga
     9961 ttattaaagt aaaaatacat ttaaagtcac gacaatatta aacctctaaa aaacgtcatt
    10021 ttcaaaccgc cattaaacag catttttaaa ccgctattga cataaaacat atttagaata
    10081 aatcattgag aaaaattaaa taattcaacc agcaataaac taactattta catgaatcta
    10141 taaaacactt agaacaaaag ttcgaaaaac gcactttatg attgtatagt gatatcaaat
    10201 atacttatca gtgagtatta agaaatactt ccattttaat actcttagaa tcaactactt
    10261 tattatctcc gtaaatattt aaaaacttat atggaacaaa aggtactagt tcttttttag
    10321 gaaataagtc aaaccaaaaa cttgtattgt tcttttcagt aaattttata tatccagatt
    10381 caaatgcaga accatcaaac tcgtatagct ttttttcttt agtgagccag tgtcttgctt
    10441 tgtaatctag ttcttgaata gtaaccatat tcttatttgt agttattgta aatccaagtg
    10501 attgtctatt gtcgattgtt acctgtacag taattaattt atctctttca ttttcagaac
    10561 tattaaatgt aagaccacca tacatacaac aacctccaaa attttggttt atatccggtt
    10621 cagatttagg tattatacat gtataaaaat atggaacacc aaaaatgtct acttttttac
    10681 ctttataatt gttagctaat tctgtatttt ctaattcagt tttaacctca ttataactct
    10741 tatactcaat tggaaaaatt aaatcatgag ataaaaactg tctagaattg ataactcctc
    10801 ttccttcaac aggtggagac atataaagat tcataacatt tcccatagtt cccttattac
    10861 ttttataatc acttacttta tttagttcgt ctattttagg atcgggttgg gaattcgcat
    10921 aatttatatt atgaaaaact atttctagaa tcaaaataat aattacagta gataatttct
    10981 tcataaaacc tcctctaact tattcagtct atttgacaat gaaacttatt ataaattcac
    11041 gcttatagat agttcaggtt gtagtgtgtc gatagcattc gttttaaaaa taaaaattca
    11101 ctagcaaaat aaattaacat tctatttatc attattacta attacaacaa ataattttga
    11161 atccgtattc aagataatat ataaatttca aattttacta ctattcaaat cgttgtcccc
    11221 tcatataata gtttattttc atgattcagt tgataatcat caatcactta atctttatat
    11281 aaaaatacat cgatatgata attagatgag ctaactgttc tattatcact ataaaattga
    11341 aaaaactctg ctcctactga accttttatg ttatataaat cataataaaa tgattgattt
    11401 tgatgattat tagaatgaaa gaaaatgcat cctttttgta tgttaccggt atctttattg
    11461 tatattttat ataaattttc taatttaaat cgaactttag tatctaattc ttgtactgtt
    11521 actttagcct ttttagtttt tataacaaaa ccttcttgtt ggataccatc tttaaataca
    11581 ttaacgccta taactctctc ttcatctaat tgatttccat catatatcgt aactcctccg
    11641 tataagcatg atgttttttc ttcagttaag cctacacatt tatttccata atacagtcca
    11701 taaatatcta tatttttccc tttaaattga tttgctaaat ctgatgagtt aaactcaact
    11761 ttcaaactag aagttttaag tacggatata tcaatatctt ttaatattat agtattattc
    11821 agtagttgat ctgtactaat tttatttgac tcgtctaatt gccacgttat atcagtataa
    11881 taacttgtta aattaaataa cttactacta tctatatcag attttttctt taaatcattt
    11941 ttgtctacat cagcattaac ataattatta tttataagac ataataaagt tattataatc
    12001 gcagctatgc agaacaatct cataaacttt ttaatatttt ttataatata atctccctca
    12061 attatttttt ggttaaatga acttctacat taatagattt actctcaact gtcttattat
    12121 cattataaat tagtaaatat ttagtcggat aaaatttttc accagattca ggcatcatat
    12181 cataccaaaa agaatgacca tttccttcga taaactttat atagccagtc tcataaggcg
    12241 aactattaaa ttcatataaa tttttatgct taagtaaata gtttctaact ttataatcta
    12301 tttcttgtgc tgttatgttt ttcttattag taggtatatc aaaagataat gaatttctct
    12361 cgttttcaaa aactttaatt aagatattat gagagttatc agttgaatta tttttatcta
    12421 tttgatttcc atcatgctcg gtcacaccgc catacataca cgttttttca attagtctac
    12481 catcatttaa ttccatatta tccgctgaaa aatagcattg ataataatag tttgtcccaa
    12541 acaaatctat gtttttattt ttgtatttat ccgaaaggct attattatta aattctgttt
    12601 ttaaaatttt agaaccatca attttagagc catttatttt aaataataaa tcatgttgta
    12661 aaaacttctc ttgggcttta atgtttattt ctgatacgtg tttatcatca tacaaatacc
    12721 tcatattatc cattagacca gtgaattcac tcgctttatt caattgttct ggtttaggat
    12781 tgccatttaa cataaatagt aagctgcaag acttaacaca tatgaagata aaagcaaata
    12841 acttcattcc aacaactcca tattttaaat ttttataaat tcgatgtatt acaggtaata
    12901 ttaaacgtac acattaccat taaaggcatt aaaatttaaa tagttaatac atatgttaat
    12961 ttcaaataaa ttcgcatttt agtgttaaaa ctaattatca ttagttacta tctacatatg
    13021 atatttcaac atcaagatga aatttttcag attctattat tttattatct tcataaattt
    13081 tcaaaaaact tacaggcagt ccatctcctg tataaaacaa atcatatgaa aatgattttt
    13141 catcatttaa atgaaataaa actttcccct tattaaaacc tgaataaaat ttagatttat
    13201 acccatattc tttaccttta ccattgttat tatgaccata tatattgtat tcttcttgaa
    13261 gatatctcct taatttaaca tcaatttctt gagctgtgac tagtttttta ttagttgcta
    13321 ttttgtcagt agaaattgtt ttatgcttgc cattaatcca aagatttata gggatttttc
    13381 tagcagaatt taagtattgt cctgataaag tggcccctcc atacatgtat ttagttttac
    13441 aaggaccatt ataatcaatg ccaaaaatat ccattttctt tcctttaaat ttacttattt
    13501 cgtcccaatt attagattct gagatcaaat cattggtacc ggttgaaaat tcaagttgat
    13561 ttgcaatagg taagttttta tctgtgacgc cttttaaatc tatataatca tattttgtat
    13621 agaaatttct taagttacct acaccaatat ctccttgcgc atacgaaagg tctttaatgt
    13681 taaaaaataa tataaaaact aatataaaac tgtatttaat ttttttcatg gcatttcctt
    13741 tactaagtta tgattgaagt atttcaactt tcgtccttat aagatatttc tacatctaaa
    13801 tggaatttat cagtttcgac agttttgttg tcattatata tttttaggaa actttcagct
    13861 tgccctgttc ctgtatcaaa taagtcataa gagaatgatg taccgtcatt caaatgaaat
    13921 gatattttcc cagtattaaa tccagaaaaa aacttggatt ttgtaccgta atttcttcct
    13981 ttatttgtat cattaaagcc ataaatgtta tattcttctt gtagatatct tcttaattta
    14041 gtatcaattt cttgagctgt tactatcttt ttattagttg atactttgtc agtagaaatt
    14101 gttttaaggt tgccattaac ccaaagatta ataggaatac atctagactt ctctaaataa
    14161 tcacccgcta aagtaactcc tccgtacata tatttatttt ttagacaata tccactataa
    14221 cttaggccgt atacatcaac agcacgattt ttgaattttt ttacatcttc aacattctta
    14281 aattcagctg ttactgtcga attatcctta gaaaaaacta attgatgtga aagacgatta
    14341 ttgtcaggat taatattttg atggtcttcg attggatagc taccataata gttcctaaga
    14401 ttcaaaactc caacatcagc ggttgcgata tgattttgtg aataacaaaa caataaaaca
    14461 acaatgataa gtattctttt cattttatat ttctcctata attaaatatg tctatcttta
    14521 ttatcctttt attgtaaaaa aacattcttc ttcttaaatc atcctgactc tatcccactt
    14581 atatactatt actcgttaat ctacttttgt tatctatact aaaatagaaa aatttttata
    14641 gtaataaagt tagatttgaa tcattaatcg ttcatcgtca tttgctatgc aactttgtaa
    14701 aaatatgtta atatctatag ttctttatag gattgtaagt tgatatgcat caacttttaa
    14761 tttgttatgt aaataaataa acatcaatat gatagtctga tgaatctatt gttttattat
    14821 cattataaat ttgcaaatat tgatctggta aatttccttt aatataaaat aaatcataat
    14881 aaaatgattc tttatgctcc gaatgagaat ggaatttaat ataacctttt tgtacatcac
    14941 tagtcattcg atcatataat ttatatacat tatttaattt tgttcttact ttaatatcaa
    15001 gttcttgtgc agtaactttc tttttatctg tagtaacgat aaatgtatta acatttacat
    15061 tatccttata cacagctact cctatttctt taggttcact taatttatta ttttcatgag
    15121 gtgttacccc gccataagta caggcagttt tcacttgatg ctcaccatga caatgcgctt
    15181 tataatagac accataaata tctacagttt ttccaaaata ctctttggaa atttccgatg
    15241 agctaaattc cacttttaaa tctttaaatt cagcagattt tccatctaac caagtaaaat
    15301 ttttaaataa taaatcaata tctaaaaatt tttctgtagt taatacagtt gactcattta
    15361 ttgtaaagcg attagtcata tagtctttat caatattatg tagagcaata tcgtctacac
    15421 ttgacttctt acacaaatcc tcaattttag gattttcttc atttgcatat gcattgttta
    15481 cactacatat tgcaattaaa tttataatta ataataatac atttaacatt gctttactct
    15541 tttttataat atccctcctt cacaaaaagc tttcttatag aaatttttca atataaatta
    15601 aaaaattctg gttttaacct ttaaatattc actaatactt tttgattacg attttaaaac
    15661 ttaacaatcc tattaacaat tgaacaaaca cattttaaac taagtgactc agataataga
    15721 caaaaaacct taaaaatagc gcttaaaatg ggtaaaacca ttttaagcgc tataacggag
    15781 agtgagggat tcgaaccctc gagacgcttg tggcgcctac acactttcca ggcgtgctcc
    15841 ttcggccaac tcggacaact ctcctcaaca ataaatatgt aataaaaaaa cagaagcgat
    15901 atttcacttc tgtttg
//
LOCUS       DAMZPP010000063        15448 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMZPP010000063 DAMZPP010000000
VERSION     DAMZPP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15448
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.063"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..653)
                     /locus_tag="POH73_001485"
     CDS             complement(<1..653)
                     /locus_tag="POH73_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI6887436.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVVLSFAWPPIQDGLNSLSNFLL"
     gene            complement(953..2692)
                     /locus_tag="POH73_001486"
     CDS             complement(953..2692)
                     /locus_tag="POH73_001486"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDI6887437.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(2732..3421)
                     /locus_tag="POH73_001487"
     CDS             complement(2732..3421)
                     /locus_tag="POH73_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDI6887438.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLIILFY"
     gene            complement(3414..3809)
                     /gene="cidA"
                     /locus_tag="POH73_001488"
     CDS             complement(3414..3809)
                     /gene="cidA"
                     /locus_tag="POH73_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HDI6887439.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKQRKGIDAYE"
     gene            complement(4023..4901)
                     /locus_tag="POH73_001489"
     CDS             complement(4023..4901)
                     /locus_tag="POH73_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6887440.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            5068..5286
                     /locus_tag="POH73_001490"
     CDS             5068..5286
                     /locus_tag="POH73_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDI6887441.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            5674..6105
                     /locus_tag="POH73_001491"
     CDS             5674..6105
                     /locus_tag="POH73_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6887442.1"
                     /translation="MKYKKILFRLLIAFAVLFSADFIYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(6245..7525)
                     /locus_tag="POH73_001492"
     CDS             complement(6245..7525)
                     /locus_tag="POH73_001492"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDI6887443.1"
                     /translation="MQSLDKNFRHLSRQQKLQQLVDKQWLSEEQFNILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHQIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASKDGQYRGIATWRYDQERQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAQVAEALKQEPRANTQVAERILQDLRSQQ"
     gene            7780..8946
                     /locus_tag="POH73_001493"
     CDS             7780..8946
                     /locus_tag="POH73_001493"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDI6887444.1"
                     /translation="MTLGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VISHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVDFDEQQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(9117..9638)
                     /locus_tag="POH73_001494"
     CDS             complement(9117..9638)
                     /locus_tag="POH73_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDI6887445.1"
                     /translation="MVYKSYYDSPVGRLELVSDGVSLTAVLFENQQDDGTREENTSLA
                     IFKEATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQIPYGTLTTYGAIAKKVG
                     KLLDKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            10001..12106
                     /gene="clpL"
                     /locus_tag="POH73_001495"
     CDS             10001..12106
                     /gene="clpL"
                     /locus_tag="POH73_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HDI6887446.1"
                     /translation="MNNNFFNSDFDSILRRMMQDMQNSNQTSNKKYYINGKEVSPEEL
                     AQFTQQGGNHSAEQSAQAFQQAALRQQGQQSGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISI
                     DISSLEAGTQYRGAFEENIQKLVEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNIMKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFETHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAIDKVT
                     TEKRISELEHDKRKAVSAEEYKKADDIQKEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDANDIERLKNISSRLRSKIIGQDKAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKEALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDTEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(12176..12346)
                     /locus_tag="POH73_001496"
     CDS             complement(12176..12346)
                     /locus_tag="POH73_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDI6887447.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDIDRDCCG
                     TEQHTANHFPGK"
     gene            complement(12359..14353)
                     /gene="feoB"
                     /locus_tag="POH73_001497"
     CDS             complement(12359..14353)
                     /gene="feoB"
                     /locus_tag="POH73_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDI6887448.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKHLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVEAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTVLKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(14365..14592)
                     /locus_tag="POH73_001498"
     CDS             complement(14365..14592)
                     /locus_tag="POH73_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDI6887449.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(14807..>15448)
                     /gene="farE"
                     /locus_tag="POH73_001499"
     CDS             complement(14807..>15448)
                     /gene="farE"
                     /locus_tag="POH73_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HDI6887450.1"
                     /translation="KTGDNDYSIKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKS
                     MGMALAFGVLFDAFVVRMMLIPALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKT
                     VESQESEAKDSKETYDTTFKVYPQGATNVSKHQDVHGQDDARSIVLDDKTMALYQEVK
                     QQSASSLFLYDALMDYQNKHQLNSKQQATNIEQLNKNIEKLNQLLEKNLRNKS"
BASE COUNT         5022 a         2930 c         2382 g         5114 t
ORIGIN      
        1 tttaataaga aattcgataa actatttaat ccatcttgaa ttggtggcca cgcaaaactt
       61 aaaaccacac ctgttgcaat tgctacgacc gatgtcacaa tcggtacaaa tcgtttacct
      121 gcaaagaatc ctaaaaatgg tggtagtgta atattataaa atttgttgta acaccatgcg
      181 gctaaagcac ccatgataat accaccaaac acacccgttt gtaatgttgg aatacctaat
      241 actaaggcat gtgctggttc tttcgctgct tgacttaatt cttttgcccc tttggcatat
      301 gagaaaatgt catcaattgt aatatgcaac actttcccca ttgttgcatt cataattaag
      361 taacctacta gcgctgctaa tgctgcaaca ccgtctcctc ctgctaatcc aagtgctgta
      421 ccaactgcaa ataataatgg caagttatca aatacaactt gtcctgctgc ttccatgacc
      481 gacgaaatca ttacaatgat atcgtttttt aaccatggtg caatttctac taattgttcg
      541 ttgtgcattg cgttaccaaa cgctaataaa ataccagctg ctggtaaaat cgcaacaggt
      601 aacattaatg ctttaccgat acgttgcaat tgtccaaaca atttcttaaa catataaaca
      661 tcccctatct caatttccgc ttccatgtac aaaaaagcag gcatgaacaa acaagactat
      721 tctcatagta cggttgtttg ctcatgcctg cattaccagt aacacgcaag tgatatgtaa
      781 ttcatagcta ttatacatat ttgtaaaagc gttttcaaca actttttcac aattttatca
      841 tcaattcagt acaatcattt gtgataaata aacaaaacgg caattcccat tttgttgaaa
      901 tagaaattac cgtttttata gttcaattca attgtgataa cctttcaatc agttataaga
      961 aacgttttgc tgcaactgaa ataggtggaa tttgatctaa atctaaatgt ttatcttcag
     1021 taatagatct aaatgcccat ttaccataac caagcgcttc ttcatttaca attttacctg
     1081 gtaatggcgc agcattagga tcaacataaa catctacaat tgttggtaca tcttgtgcta
     1141 gtgcctcttc tacaatagca tctacttcgc tagcactttt aattgtataa cctttaccac
     1201 ctgctgcttc agcaaatttg gcatgatcca tatcagagaa atcaaccgca tattctaatt
     1261 caccagcagc ttgttgttca tatttaataa acgctaattg cttattattt agaacaaaca
     1321 ctgttaaagg tagatcatat tgtacagctg ttgcaaagtc ctgcatcacc atttggaatg
     1381 caccgtcacc agcaattgcg atggcttgtc tatttggata tgcaattttt gatgccatcg
     1441 cacctggaag accgcaaccc attgtaccta accaacttga aatgatgaac ttgttattta
     1501 caccaaggtt taagtatcga gttgaccaaa ctgttgctgt acctacatct gctgaaatca
     1561 ctgcatcatc tttaataaat ttattgattg atgccattaa tcgttctgga cgtaatggtt
     1621 tactattatt atttttatct tgttccatcc atttatccca aaccgcttta cgttctaacg
     1681 ttttgtttaa gaatggtctt tcagcaacat gtttaatatt ttcagttaac tgatgcaacg
     1741 caattttact atctccaaca attcctacat taatattgaa acgatgtccg atatttttag
     1801 gatttgtgtc aatttgaatg gctttaatat ttttcttagg taagtaatcc acatatggat
     1861 agtttgtacc aaccataatt aataaatccg cttcctgcat tgtttgataa gatgttttag
     1921 taccgatttt acctaagtta ccgatactat atggatgatc atccggcaag attgttttag
     1981 ctggtaatga atgaatgaca ggaattttag ccatttcaat aaattcacgt agctcatctt
     2041 tcgcatgttt cgcaccaaca ccgattaaca tgacaggctt tttactttta ttaattaatt
     2101 taaccgcttt tttgatgtct ttatattttg gtgatactac tgttggtctt gatgtatcta
     2161 ctggtttatt cgttgtatct ttaatttttt cagttaataa gtcattagga caaataacaa
     2221 cagctacacc tttttgttca tatgccgtac gaattgcttc gttaacgatt tcaaacacat
     2281 tgtcaccttt ttcaatttgg tgattataaa cggctacatc ttcacataat ttttgtaaat
     2341 ttgtttcttg gaatgctttc gttccaagtg ctgtactatt cgtttgtcca gataatatta
     2401 attgcggtac attatccatt ttggcatcgt acataccatt taataaatga attaaaccag
     2461 ggccaccgat acttaatgcc acaccgattt taccagttaa ttttgtataa ccagcagctg
     2521 ctaaacttgc tacttcttca tgacgtacat gataaaattt aaattgatct ctcactgtac
     2581 gtaaactatc gaccactgcg tcgattgaat cacctggaat accatataag tgatctatat
     2641 cccatgcttg taatgcttta actaatgctt catttgcttt tatttttgcc attattacta
     2701 atagcctccc tttcttagtg tctttttcta attaatagaa cagtatgatt agaacaggta
     2761 ttaacaccga actaataact gcagttaaaa tcatcccaat tgaactaaat gcaccagatt
     2821 caatatccat ctccagtgct ttagctgtgc caaatgcatg tgacgcattc ccatacgtta
     2881 aacctttggc gatagaagat tcaaatcttc caaatcgtaa taacatcgaa cctaaaatac
     2941 taccgattaa acccgttgtg ataataaaaa gtacggtcat cgtatctgtg ccaccaagtt
     3001 catgtgacac ttctatacca acagcggctg ttatagatcg gggcaataat gttacaatga
     3061 cgtctttaga atagccaaat gctttaagtg ttaagaacac taacatgaaa tttagcatga
     3121 cgccagttaa tacacttgca aaaatgatag agacattatc tttaattttc tctctatttt
     3181 tataaagtgg gtacgctaaa catacgaccg ttgcgtttaa aatatggttg atccaactgc
     3241 cacctttcat atacccgtta taactaattc caaagataag taagacaaaa ataattccta
     3301 aagatgcaat taatgctgga ttcaaaaatg gatttggata tttttgttgt aatcttttag
     3361 cgaaataata taaaacgaca gtcaataaaa tcattaacaa ggcttgcacg taatcattca
     3421 taagcatcta tacctttacg ttgcttatgt ttaacagaca ttttttcagc aatataacct
     3481 gaagataatg caacgataca tgttcctatg ataatgactg cgaaaaagag tatataattt
     3541 agcgtaattt cggaagcaac atccataata cctacaactg acggtatgaa gaaaaagacc
     3601 atcgtcttta ataaaaagtt tgcaccgtct tctacccaag ttagcggtac aatcttaaat
     3661 tgtaatagta aataaaataa aaatagacca acaatactgc cggctaaggg aagatgaaaa
     3721 atcttttgaa tttctgtgcc aatataagta atcacaatga tgattcctag ttgtagtaat
     3781 aatttgatta ttaattggac tttgtgcatg gcgccatccc tttctaaata tgtctaaatt
     3841 gttacaataa ctattataaa gatggcgtac acaatataaa aatactcttt ttttatggtt
     3901 tgtattacta ttttctatgg aaacgctctc taaatttcac ttcattttca caaaaaaaac
     3961 actcattatt tcaatgatcc aagtaatgag gaatcacaaa taatgagtgc tttattaata
     4021 tgttagccta aacgatcttt caaaaattct atccatttcg ttgtggcatg acttaattgt
     4081 ttatccttct tccaaacaac acctaattcc caatgtacat gcgcatcttc aatgcgtagt
     4141 agcttcacat ctccatttag ttgctctgaa attgatgttg gtaaaatact aatacctaat
     4201 tcattcgtaa ctaaatcttc tataacatgc cattgtgaaa tttgcgctac agtattcgga
     4261 acaaagccaa cgtttttagc attttcaata attttatcat tcaagtaaaa gtctttatta
     4321 aataaaatga agtcttcacc tgctaaatct tcgagtttaa cagtttcata ttttgcgagt
     4381 cgatgctctc tgctcacgat aagtcgcaaa tcttccttat ctagggtagt ataatcgaaa
     4441 atatgatgat ccactggcaa agtggtcacg cctatatcta cttcatcatt gataatttgc
     4501 tgctcaatcg tttttccgcc attttctatt aaattatatg taacatttgg atattgttga
     4561 tggaatgcac caagaatatt tataagaatc ttcatattca tgactgccga catgcccatg
     4621 tttatatgcc ctgtttccag tccattcaag cgttccattt cagatggtaa ataatcgtat
     4681 agtgcaacaa tttctttact tttctcataa aaaatttgac ctgcatcggt aagtattaaa
     4741 tgtcttttac ttctatcaaa taagggcgta cccatttcat tttcaatatc tttaattgct
     4801 ttactaattg taggttgtgc aatatataat gatttggaag cattagtcat gcctccttgc
     4861 tttacgactt caataaaata tttcatatgt ttgatatcca ctaacatcat cctaactatt
     4921 tcaacacgtt tatttcatta tacacaactt cttaaacatt caataaattt agatttataa
     4981 aattgcttta taaaaagttt tatgtaaaat acacattctt ttatggtatt atattaatgg
     5041 ctcaagtttg ttattcgagg tgaggtattg agcttttacg aatttatgca aagttttctt
     5101 ggtgatgaca caccattagg tgaattggtt gattggatta atcaagatag caatttccct
     5161 agagaagtga agagccatag tgaaatattg tcatattttc gaacaaatcc atgtcctgaa
     5221 agcatctcag tacctgtaat taaacgggca ctatcagttt tcaaccaatt cacaaatgta
     5281 caatgataca tgaatacatg gagtcacatc atctcatgtt agcgctctga ttgcagggcg
     5341 ctgtttttaa tgttatcgct tcatttatat gttgtgattg ctattttctt tcagttatag
     5401 aaccacgtac cttttctttt aaatcatttt atattttccc actaatatcc gctgttaatc
     5461 aatcctgaca tccttgtatc actatgacac ttaattgtta gatacataaa tttctacatt
     5521 ttatgaaaaa atccattttt attacaattt aacactttat atgacagctt cattacagtt
     5581 acttttattg ttgattgctt gcattgtttt ctaaaaaaat tttgctatca taaataacgt
     5641 tgaataaaga aaaaattaac ttgggagata aaaatgaaat ataaaaagat actttttcgt
     5701 ttactaattg cttttgcagt ccttttctca gcagatttca tttatcaatc cgtcgaacaa
     5761 acgcatcaat cgcatgccgc agttaattat tatagtaaaa accaatgtac atggtgggca
     5821 tttaaacgtc gcgcacaagt cggtaaacct gtttctaata gatggggcaa tgccaaaaat
     5881 tggtattaca atgcacgtaa atcaaaatat gcgactggtc gtacaccaag aaaatttgct
     5941 gtcatgcaat caactgcagg atattatgga catgtcgcag ttgttgaaca agtatataaa
     6001 aacggtagta ttaaagtttc agaatacaac ttttatcgcc cattaaaata caatacacgt
     6061 gtactaagca aaaaggcagc acgtaacttt aactatattt actaatcaaa aaacttctat
     6121 cacgaacgct tcaaattcct gtatgcgtgt gatagaagtt ttttattttt tgttcatatt
     6181 aatttattta agcgctacga tggactgact taaacttttc tttaaaatcg ctattcgcat
     6241 ttgattattg ttggcttctt aaatcttgta aaatgcgttc agcaacttgt gtattcgcac
     6301 gaggttcttg cttcaatgct tcagctactt gcgcaatttc atcacctttt gcacctacaa
     6361 caatagctaa agatttatat tgcaagctca tatggccttg ctggatacct tcagaaacga
     6421 gcgcgcgaca tgctgcaaag ttttgtgcta aaccaacggc agcaactaca tgacctaatt
     6481 cttgcgctga ctctacattt agtagctcta atgatgcttt agctattggt aatacttttg
     6541 taccaccgcc aacgattgcc aatgtcatag gcacctctat ggtaccaatt aaacgttgac
     6601 gttcttgatc gtatctccat gttgcaatac cacgatattg accatcttta ctcgcgtatg
     6661 catgcgcact tgcttctgca ccacgcgtat catttcctgt tgctaaaaca acggcatgta
     6721 tgccattcat aacaccttta ttatgtgttg cagcacgatg aatatcaact tgtgccaata
     6781 cagaagcacg ttccattcgt ttggcaacct cttctccagt tctctcgccc cttgctaaat
     6841 ctttaacatc aatttcgcct tgaactttaa caacggacgc tgttgcatga ttggataaaa
     6901 tactcattaa aatgtcgctt tgcggaaatt cattttttaa aaatgcagtt atagcctcta
     6961 aaatcgtatt aagcatatta gcgcccatag catctttcgt atcaacaaat acttttaaag
     7021 atagtaactg ttgctcagga aatgtatcaa tagctatacg ttggtaacca ccaccacgcg
     7081 ctttaataga aggatatgcc tcatccgcaa tttgatgaat ttgcttctct aaagctttaa
     7141 tgtctgctga taatttttca gtatcgtcaa cgccatcaaa gacgatttga cctatcataa
     7201 tacgttcaga agataccgtt ttaaatccac cagtctgatt cactagcttt gcaccataac
     7261 tagctgcagc gacaactgaa ggctcttcca ccatcatagg tacaacatat gctttatcgt
     7321 ccacaatgat attcggtaat aatccaacgg gtaatgcacc ttgcgcgatg acattttcaa
     7381 ttaaactatt tgctacttcc tcatcaatta atggatgatt caataaaatg ttgaattgct
     7441 cttctgataa ccattgctta tctaccaatt gttgtaactt ttgttgacga gataaatgtc
     7501 ggaaattctt atctaaactt tgcatggaca tactcctttt acttcacata attttttaac
     7561 atttcaatca ctactatttt taccataaaa taacgttatt cgtcttaaaa ttcaattgaa
     7621 taattgtcgt tttgacttta aaataaagca aggtaaatta aaacgcttac aagaaacgtc
     7681 aaatcatttt taaatttagt gtatttcttt gtataagatt agcatattct gatatgatac
     7741 aagtgttgct tttataaatt tgaaaggatg taaaacctta tgacattagg tatcgacaaa
     7801 ataaactttt acgttccaaa gtactatgta gacatggcta aattagcaga agcacgccaa
     7861 gtagacccaa acaaattttt aattggaata ggtcaaactg aaatggctgt tagtcctgta
     7921 aatcaagaca tcgtttcaat gggcgctaac gctgctaagg acattataac agacgaagat
     7981 aaaaagaaaa ttggtatggt aattgtggca actgaatcag cagttgatgc tgctaaagca
     8041 gccgctgttc aaattcacaa cttattaggt attcaacctt ttgcacgttg ctttgaaatg
     8101 aaagaagctt gttatgctgc aacaccagca attcaattag ctaaagatta tttagcaact
     8161 agaccgaatg aaaaagtatt agttattgct acagatacag cacgttatgg attgaattca
     8221 ggcggcgagc caacacaagg tgctggcgca gttgcgatgg ttatttcaca taatccaagc
     8281 attttggcat taaatgaaga tgctgttgct tacactgaag acgtttatga tttctggcgt
     8341 ccaactggac ataaatatcc attagttgat ggtgcattat ctaaagatgc ttatatccgc
     8401 tcattccaac aaagctggaa tgaatacgca aaacgtcaag gtaagtcact agctgacttc
     8461 gcatcactat gcttccatgt tccatttaca aaaatgggta aaaaggcatt agagtcaatc
     8521 attgataacg ctgatgaaac aactcaagag cgtttacgtt caggatatga agatgctgta
     8581 gattataacc gttatgtcgg taatatttat actggatcat tatatttaag cctaatatca
     8641 ttacttgaaa atcgtgattt acaagctggt gaaacaattg gtttattcag ttatggctca
     8701 ggttcagttg gtgaatttta tagtgcgaca ttagttgaag gctacaaaga tcatttagat
     8761 caggctgcgc acaaagcatt attaaataac cgtactgaag tatctgttga tgcatatgaa
     8821 acattcttca aacgttttga tgacgtagac tttgacgaac aacaagatgc tgttcatgaa
     8881 gatcgtcata ttttctactt atcaaatatt gaaaataacg ttcgcgaata tcacagacca
     8941 gagtaatctg tgtatttgaa agacatttaa atataaccta aaaacagcag taagacagct
     9001 tctcattgaa atcgtcttac tgctgttttt tatttataat gcttcgtctt gaatgaattc
     9061 ataatgccta tttgacacat tattaaagtt ttcctattgc ctgaatcctt tatacgttac
     9121 ggcttcgtgc tatgttttgg tacataaagc tttgacatat cgatattctc caactctaac
     9181 agcttaattt tattattaat cgttccaccg aatcctgtta agctacccgt tttaccgaca
     9241 acgcgatgac atggcacgat aatagataat ggattacttc cgactgcacc tccaaccgct
     9301 tgggctgaca tttttggctt atcaagcagc ttgcctactt ttttggcaat agcaccatac
     9361 gttgttaacg tcccataagg aatctgtctt aattcattcc aaacacactg ttgaaaatga
     9421 ctacctgttg gctttaaagg tattgtgatt tcaggattgt cacctttaaa atacgcgtct
     9481 aaccactgtg tcgcctcttt aaatatcgct aaagacgtat tttcttccct agtaccatca
     9541 tcttgttgat tttcaaacaa aacagcggtc agacttaccc catcactcac aagttccaat
     9601 cgtcctacag gagaatcata gtaactctta tataccataa acacacactc ctttttcatc
     9661 atcatactat tcatttaact ttatgaaaat gattaacact ttatgcttta tcaagatcaa
     9721 ctattcactt ccaaatcaaa attcacatag actaccaaca caatatacca tttcttaatt
     9781 aactatgtat caacacatac gtttcttaac cattgattaa tatttacttt taaattctta
     9841 cttacttcaa aaattacaca aaaagttcat aaagtgccta caatgcgccg tttttaaaac
     9901 tacaatggtg gaaaaataga attgccattg tttatagttt gcatatagtt tcaatatatt
     9961 ttcttttact taatagttta aaattttaga ggtgatgttt atgaataaca attttttcaa
    10021 tagtgacttc gattctattt tacgaagaat gatgcaagac atgcaaaact caaatcagac
    10081 tagtaacaag aaatactata ttaatggtaa agaagtttca ccggaagaat tagcacaatt
    10141 tacgcaacaa ggtggtaatc attctgccga acaaagtgcg caagcttttc aacaagcagc
    10201 actaagacaa caaggtcaac aaagtggcaa tggcaattat ttagaacaaa ttggtcgtaa
    10261 cttaacgcaa gaggctcgtg acggattatt agatccagtc atcggtcgtg ataaagaaat
    10321 ccaagaaact gcagaagtct taagtagacg cactaaaaac aatcctatcc tagtcggaga
    10381 agctggagtt ggtaaaactg cgattgtcga aggtttagcg caagcaatcg ttgaagggaa
    10441 cgtacctgca gcaatcaaag ataaagaaat tatatctata gatatttcat cgcttgaagc
    10501 tggaacgcaa tatcgtggcg cctttgaaga aaacatccaa aagttagttg aaggcgttaa
    10561 gtcatcacaa aatgctgtgc tcttctttga cgaaattcac caaatcatcg gttccggtgc
    10621 cacaggaagt gattcaggta gcaaagggtt atctgatatt ttaaaaccag cattaagtcg
    10681 tggtgaaatt tcaattatag gtgcaacgac tcaagatgaa tatcgcaata atatcatgaa
    10741 agacgctgca ttaacacgtc gttttaacga ggtactggtt aacgaaccaa gcgcaaaaga
    10801 tacggttgaa attttaaaag gaattcgcga aaaatttgaa acacaccatc aagtcaaatt
    10861 acctgatgac gtattaaagg catgtgtaga cttatccatt caatatattc cacaacgcct
    10921 attacctgac aaagcaatcg atgtattaga tattacagct gcacatttat ctgcacaaag
    10981 tcctgctatt gataaagtca cgactgaaaa acgtatttct gaattggaac atgataagcg
    11041 taaagctgta agtgctgaag aatataaaaa agcagatgac attcaaaaag aaattaaatc
    11101 tctacaagat aaattagaaa acagtaatgg tgaacatact gcagtagcaa cggtgcatga
    11161 tatttctgat accattcaac gattaactgg tattcctgta tctcaaatgg atgccaatga
    11221 cattgaacgt ttaaaaaata tttctagtcg tttaagaagt aaaatcatcg gtcaagataa
    11281 agctgtagaa atggtttctc gtgccattcg tcgtaatcga gctggttttg atgatggtaa
    11341 tcgtccaatt ggtagcttcc tatttgtcgg tcctactggt gtgggtaaaa cagagcttgc
    11401 caaacagtta gccattgatt tatttggtaa taaagaggca ctgattcgac ttgatatgag
    11461 tgaatatagt gatactactg ctgtttctaa aatgattggt acaactgctg gttatgtcgg
    11521 ttatgacgat aattcaaata cattaactga aaaagtacga cgcaatccat attcagtcat
    11581 tctatttgat gaaattgaaa aggctaatcc acaaatatta acattattac ttcaagtgat
    11641 ggatgatggt aatttgactg atggtcaagg taatgtcatc aactttaaaa atacaattat
    11701 tatttgtaca tcaaatgctg gctttggcaa tggcaatgac actgaagaaa aagatattat
    11761 gcatgaaatg aaaaaattct tccgccctga attccttaac cgcttcaacg gcatcgttga
    11821 attcttacat ttagataaag atgcattaca agatatcgtc aacttgttat tagacgatgt
    11881 acaagttaca ttagataaaa aaggtattac gatggacgtt tcacaagatg cgaaagattg
    11941 gttaattgaa gaaggttatg atgaagaatt aggcgcacgc ccattaagac gtattgttga
    12001 acaacaagtg cgtgacaaaa taacagatta ttatttagat catacagacg ttaaacatgt
    12061 ggatatagat gttgaggata acgaattagt cgtaaaaggt aaataacgac aatttaacat
    12121 atagcgtatc aaaaaatgag catcaggacg ccctcgcctg tgctcatttt ttaaattatt
    12181 tcccaggaaa atgattcgct gtgtgttgtt ctgttccaca acaatcacga tcaatgtcac
    12241 atgtaccaca ttttccttgt ttcgaacgtt tgaaaaattt cactagtgta tacaaggcat
    12301 atccgaaaat tgctaaaaaa attaaaatgt taataatgac tgacacttta accactcctt
    12361 aaacaaataa atgtccgact tgataaaaaa tgaatgttaa gacatatgca gtgactagag
    12421 gataggcaac tgcaagtgcc gtccatttcc atgaataagt ctctttacgg attgctgcca
    12481 ctgtagaaac acaaggaata tacaatagta taaatatcat aaatgcatac gcagatagcg
    12541 gtgtgaattg attttgaatc acattaacaa ggcctgcatc acctgatgaa tagataatcg
    12601 ccatcgaact tacgataact tcttttgcta aaaatcctgg cactaacgta gcacctgctt
    12661 gccatgttcc aaatccgagc ggttgcacta acataccaaa gaagctacca accatatgca
    12721 aaaaactttg attgatattc acattgatac catgtggtcc tacataactt aatagccaaa
    12781 tgactactga gccgccaaaa ataaatgtac ctgctttacg aacaaagccc ttagcctttt
    12841 cccaagtact acgccacaac gttttaatgg aagggacacg gtatgttggc aactccacaa
    12901 taaagattgc attatcattt tttaaaactg tcttagtaag tactgtactg actaaaaatg
    12961 ccataataat acctaaaaca tacaagctta atactactaa agattgattt tctttgaaaa
    13021 agatacctac gaacaatgca tacactggca gtctagcaga gcatgacatg aatggtgcaa
    13081 ttaatatcgt tgttaaacgc tctttttcat tttcaatact gcgcgcagcc ataatactcg
    13141 gtacgttaca accaaatccg ataatcatcg gtataaaaga cttcccgctt aaaccgaacg
    13201 attccataat acgatccatt aaaaccgcaa tacgtgccat ataacctgaa tcttctaata
    13261 aagatataaa gaaaaagagc acaacaattt gtggtacaaa gactaatact gatcctacac
    13321 cagcaataat gccatctgta attaaatctt gtaaaaatgg tataacgcca agataattca
    13381 taatcgtctt cacactatcc gtaaatgtac cacctataaa tgcatcgagt tgatccgaca
    13441 aaggtgtgcc aatccatgta aatgtagttt gaaagatcaa ccacataatc gctagaaaaa
    13501 taggcatccc tatatattta tgtgttaata tcttgtctat tctagagctg aaatattgct
    13561 tatcttcatc tggatacgtt accacgtctt gcaataacgt ctcaatataa tgattgcgta
    13621 tacgttccat ctctcgacga acagatacag cctctacttg ttcagcaact tgatcacgta
    13681 aactcgacaa tttatttaca acctctgaat taagttcatt tgcaatttgc atgttattta
    13741 gtaagaattg aatcgcaata aatctagctt gatatttatc atgagatgtc tctgtcatta
    13801 ttatttgaca catattttta attgtctctt caatcttctc accataattg attttaaaat
    13861 gcggttgata cccttcccct agatgcttta tttcgccaag taaatgttta gttcctttgc
    13921 ctgtacgtgc cacaactgga aaaataggtg tttttaactt tttcatcaat ttatgataat
    13981 cgatttttat cccgcgcttt gtagctacat caatcatatt taatccgatg tatattggtt
    14041 gattaagttc taacaattgt actgttaatt gcatatttct ttttagttga ctcgcatcaa
    14101 caatgttaat gattcctgaa aaagaatcgt ttaataaata gtctgtcact acagtttcat
    14161 ctttagaaat cggcgataaa tcatatgtac ctggtaaatc aattaattgt cctacatttt
    14221 ctttaagctt ccctactttt ttctctaccg ttacgccact ccagttgcct atatattcat
    14281 acgaaccagt taaagcgtta aacaaagatg ttttaccaac attaggattt cctaaaatac
    14341 aataattttc cattcgtccg gctcctattc ttctaatgca atagaacaag catcgcaatg
    14401 tctaatactt aactgttgtc cgtttacttc aataatacat ggccctttaa ataaacattt
    14461 ttgtttaatc gttatgatag cgtcatctgt taacccaaag gcacttagac gatacaacat
    14521 attctcatta gcaatatcca ttcgctttat tttataagcc ttattcattt caccattttt
    14581 aatgtttaac atactatttc gctctcctat tagaaataat aatcattatc acttaaaaat
    14641 cataaccctt aaaattgtag ctcgcaatac tttatttaaa taattttcat ttttcatgta
    14701 aaatttgtga cattgcaaaa atgttttgta taatcgcttc catgcaaaaa cccccacttc
    14761 aaatgtcatt gatagacatt taaagcggag gcatgtacat gttattttat gatttatttc
    14821 ttaagttttt ttctaacaat tgattgagct tttcaatatt tttattcaac tgttctatat
    14881 tcgtggcttg ttgttttgaa tttagttgat gcttgttttg ataatccatt aaagcatcat
    14941 ataagaacaa tgaacttgct gattgctgtt tcacttcttg gtacaatgcc atcgttttat
    15001 catctagtac tattgaacgt gcatcatctt gtccatgtac atcttgatgt ttcgatacat
    15061 ttgttgcacc ttgtggatat actttaaagg ttgtgtcgta tgtttcctta ctatctttag
    15121 cttcagattc ctgtgattca accgttttat atttttcaag tgcatgtcct tcaatatcaa
    15181 ctcgtggaat aatacgattc aaccatgctg gtaaatacca cgaacctttt ccaaacaatt
    15241 tagttaatgc aggaattaac atcattctga ctacgaaggc atcaaagagt acaccaaacg
    15301 ctaatgccat acccattgat ttaatcatga catcttcttg gaatacaaac gcaaagaaga
    15361 cactaaacat gattaatgca gctgctacaa taacaggacc actttctttc aatcctactt
    15421 tgatagaata atcattatcc cctgtttt
//
LOCUS       DAMZPP010000064        15268 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMZPP010000064 DAMZPP010000000
VERSION     DAMZPP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15268
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.064"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..1486
                     /locus_tag="POH73_001500"
     CDS             <1..1486
                     /locus_tag="POH73_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDI6887451.1"
                     /translation="GFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWA
                     NWVSLVTIIPIEAVSAVQYMSSWPWHWAKPMKHLMENGSISTYGLLAVYLIIVIFSLL
                     NYWSVKLLTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAA
                     TTASGIIFSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQ
                     SMLQHSGWNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRA
                     MEKNGHIPKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYL
                     TGPTTVIALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILG
                     LPIYFFYEYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIV
                     IIIVALIFYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSY"
     gene            1793..3145
                     /locus_tag="POH73_001501"
     CDS             1793..3145
                     /locus_tag="POH73_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HDI6887452.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSATFTEHEDCLYLNIWKQHNDQSKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKGFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSIWTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKLMLDYLDTDDVTSLSTDDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(3207..4394)
                     /locus_tag="POH73_001502"
     CDS             complement(3207..4394)
                     /locus_tag="POH73_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6887453.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGSLGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(4788..5564)
                     /gene="fetB"
                     /locus_tag="POH73_001503"
     CDS             complement(4788..5564)
                     /gene="fetB"
                     /locus_tag="POH73_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDI6887454.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLVLCVFVIIVNASWNTISRSSPVMHHVFLISFVAIFVGTALP
                     LVGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TTKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            complement(5557..6219)
                     /locus_tag="POH73_001504"
     CDS             complement(5557..6219)
                     /locus_tag="POH73_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6887455.1"
                     /translation="MLLEIKDLVYEASDRVILNHINLKVDKGETIAIIGPSGSGKSTF
                     QKLICNLISPTSGTLYFKGKPYDDYEPEILRRHISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDKKRAKQLIKDVGLGHYQLSSKIEHMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDINNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNTEELNQ
                     HE"
     gene            complement(6565..7641)
                     /locus_tag="POH73_001505"
     CDS             complement(6565..7641)
                     /locus_tag="POH73_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDI6887456.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLTNDKYLTAKAFDNRYGCALAVEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKKHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSVRLVTEIVRSLNDETYKNIMW"
     gene            7879..8334
                     /locus_tag="POH73_001506"
     CDS             7879..8334
                     /locus_tag="POH73_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDI6887457.1"
                     /translation="MKKLCSLIVVALVCVIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            complement(8502..10079)
                     /locus_tag="POH73_001507"
     CDS             complement(8502..10079)
                     /locus_tag="POH73_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HDI6887458.1"
                     /translation="MTFLTVMQFIVNIIIIGFLLTVMVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLTNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            10275..11024
                     /locus_tag="POH73_001508"
     CDS             10275..11024
                     /locus_tag="POH73_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDI6887459.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRRMHEDAFVPMIRMQIVASA
                     KYKWHILISFTLSIVLFLLLNHFAIFALNVDSFTVFDIYPLIREYGSISLIAIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSVILETLYYYPYINTWWEFIPALILAS
                     SATYLYLKSNRNFMVSYFYQLAVILVLHIFM"
     gene            complement(11248..12441)
                     /locus_tag="POH73_001509"
     CDS             complement(11248..12441)
                     /locus_tag="POH73_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6887460.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVVARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSLLKKPTPR
                     PANSL"
     gene            complement(12453..13202)
                     /locus_tag="POH73_001510"
     CDS             complement(12453..13202)
                     /locus_tag="POH73_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6887461.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKDVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            complement(13195..14010)
                     /locus_tag="POH73_001511"
     CDS             complement(13195..14010)
                     /locus_tag="POH73_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6887462.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIVGLNPERLRVTGDITFDGKSMLSLSETQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEHGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            complement(14007..14876)
                     /locus_tag="POH73_001512"
     CDS             complement(14007..14876)
                     /locus_tag="POH73_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6887463.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            complement(14873..>15268)
                     /locus_tag="POH73_001513"
     CDS             complement(14873..>15268)
                     /locus_tag="POH73_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6887464.1"
                     /translation="VIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVKSITLML
                     HVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPVIQAYVL
                     IVAVLFIVFNTLADIINALLNPRLREGAR"
BASE COUNT         5078 a         2949 c         2274 g         4967 t
ORIGIN      
        1 tggattccta gtcattggaa ccattgccta taactacatt gaaatcggca caatgtttcc
       61 tcaatcaggt ggcatgagta actatgccca gtatacacat ggctcattat taggctttat
      121 tgctgcttgg gcgaattggg tgtctttggt gacaataata cctatcgaag ctgtgtcagc
      181 tgttcaatat atgagttctt ggccgtggca ttgggcgaaa ccaatgaaac atttaatgga
      241 aaatggctct attagcacat acggattgct agctgtatat ctcatcattg ttattttttc
      301 tttattaaac tattggtccg taaaactttt aacatcattt acgagtttaa tttctgtatt
      361 taaattaggc gtacccatgt taaccatcat catgttaatg ttatcaggat tcgacacttc
      421 aaattacgga cattcagcaa gcacatttat gccttacgga agtgcaccga tttttgctgc
      481 aacaacagca tcagggatta ttttttcatt caattcattc cagacaatta ttaatatggg
      541 ttcagaaatt aaaaatcctg aaaaaaatat cgcaagaggc atcgctattt cactgtcaat
      601 cagtgcagtg ttgtacatca ttttacaaag tacgtttatc acttctatgc ctcaatcaat
      661 gttacaacat agtggatgga atggcatcaa cttcaattca ccatttgctg atttagctat
      721 cttattagga attaattggc tcgcaatttt actatatatt gaagcttttg tatcaccatt
      781 cggtactggc gtctcatttg tcgccgttac aggtcgagtt ttacgagcaa tggagaaaaa
      841 tggacatatc cctaaatttc ttgggaagat gaacgaaaaa tatcatattc cacgtgtagc
      901 aatcatcttt aatgccatca ttagtatgat tatggttacg ttatttagag attggggtac
      961 gctagcagca gttatttcta ctgcaacttt agtagcctat ttaactggcc caacgacagt
     1021 gattgcatta agaaaaatgg gaccaacaat gactcgtcca tttagagcaa aaattttaaa
     1081 agtaatggca ccattatcat ttgtattagc ttcattagct atatattggg caatgtggcc
     1141 aacaacggct gaagtaattt taatcattat acttggctta ccaatctact tcttctatga
     1201 atatcgtatg aattggcgta atacaaagaa acaaattggt ggtagcttat ggattattgt
     1261 atatttaatc gtgctatcaa tactgtcatt tataggaagc aaagaattta aaggcttaaa
     1321 tatgattcac tatccatttg actttatcgt tattattatt gtggcgctta tcttctatta
     1381 catcggtaca acgagttcat ttgaaagcgt ctatttccgt cgcgcaacac gaatcaatac
     1441 aaagatgcgc gagtcactaa ataatgaatc aaagtcatct tattaaatat tttaaaatgc
     1501 agtgccatat atgatttcaa cacacacatt aaccaaccat tgatttcaac atcttggttg
     1561 gttttttatt ttgaaaatgg gttataaata actaacataa caagatagcg agagtgatat
     1621 tgtaatgaaa tgtcactgta gaagaacatc atataatgct gaaaatggtt tgagttaaaa
     1681 cagatatcaa gctcgtctga ttcagccaca aaattgtctt gttatacttg tcacctatca
     1741 tccatagacc gtggtatgat taaattgggg atgataaagg aggttaataa atatgaagat
     1801 taatactaca ggtggtcaaa ttcatggtat tacacaagat ggtttagata tcttcttagg
     1861 cattccttat gcagaaccac cagttcatga caatcgcttt aaacattcta cgttaaaaac
     1921 acaatggtca gagccaattg atgcaactga aatacaaccc atcccaccgc aaccagacaa
     1981 caaattagaa gattttttct cctcacaatc tgcaactttt actgaacatg aagactgttt
     2041 atatttaaat atttggaaac aacataatga tcagtcgaag aaacctgtca tcatttattt
     2101 ttatggtggt agttttgaaa atggtcatgg tacagccgaa ctctatcaac cggcacattt
     2161 agtacaaaat aacgacatta tcgttattac atgcaattat cgtttaggcg cattaggata
     2221 tttagactgg tcatatttta ataaaggttt tcattccaat aatggacttt cagatcaaat
     2281 caatgtcata aaatgggtgc atcaatttat tgaatccttc ggtggcgacg ctaataacat
     2341 tactttaatg ggtcagtctg caggcagtat gagcatttgg actttactta aaatacctga
     2401 cattgagcca tacttccata aagtcgtttt gctaagtggc gcactacgat tagacaccct
     2461 tgagagtgca cgcaataaag cacaacattt ccaaaaattg atgctcgatt atttagatac
     2521 agatgatgtt acgtcattat cgacagatga tattcttatg ctgatggcga agctaaaaca
     2581 atctcgagga ccttctaaag ggcttgattt aatatatgcg cctattaaaa cagattatat
     2641 acaaaataat tatccaacaa cgaaaccaat ttttgcatgt tatacaaaag atgaaggcga
     2701 tatttatatt actagtgaac aaaaaaaatt atcgccgcaa cgctttatcg acattatgga
     2761 attaaatgat attcctttaa aatacgaaga tgttcagacg gcgaagcaac aatctttagc
     2821 gattacacat tgttatttca aacagccgat gaagcaattt ttacaacaac tcaatataca
     2881 agattcaaac gcacaactat ggcttgctga atttgcatgg catgatactt ctagtgcaca
     2941 ctatcgcagt gcttaccata ttctagatat ggtattttgg ttcggaaact tacaaatatt
     3001 agcagcacat caatatccaa caactgctca ccttaaattt ttaagtcgac aaatgcaaaa
     3061 tgatttagct aattttgcta aaagcggtaa aatgccttgg ccgatgtatc ataatgaacg
     3121 tcgctattat cgcacatatc aataacgtta tcgcatctaa aaaagcacac ctgaatttga
     3181 tagcaggcgt gcttttcata tgagaattat gatgatttaa atgttttagc ataatgatta
     3241 tttttcaatg tgaaaacaac gattaatcct aatgagccaa tgatggcact cgtatacgtg
     3301 atagcttgaa cagaaacatg tgtcatgacc aagcctccaa taatgcctcc aacaccaata
     3361 ccagcgttta aactagacat gttccaactc attacttggc ttgtgtcgcc ttcaacatgt
     3421 tgaatcacac cgctttgcac tgctggatta gtactccatt gcatgatatt ccaaataaat
     3481 agtcctgcta acaatagacc tgaaccaggt aagatcaaat tcataagtaa catcatcacg
     3541 ataaaaatag aaacagaaat cattaaccaa cgcttacttg taattttatc agagaatata
     3601 ccacctaatg atgttccaat aacgccagcg attccattta ctagaagtgc taatgaaacg
     3661 aatgacatat catgaccatt agataaaata agtggattta taaagacgaa tgtcactgag
     3721 tttgcaatca atactaaaaa cgtaataatt aaatattttg ctacttcagc aggtcttaat
     3781 attttcgaag taacatgatt ttcatgagat ggtgcctcat gattcacagg gcctcgttgt
     3841 atttcctgat ccttcggtaa atagatcatc attaagaagc caacaataat actcacaata
     3901 attaagaata gaaatgtata gcgccaacct actaaatcgc cgataaccgt tccaattggt
     3961 acaccaaaaa cattggcacc actaaaccct gtatagacaa gtccaatcat tttgccacga
     4021 tttttaggtg ctgataacat cgctgtaata gctaatactt ttacgataat tagtgctgct
     4081 gctgcagatg agataattct accaactact aatattgaaa aatttggcgc tacagcaata
     4141 atgccattac caatgataaa tataagtaat gtccataata atacaggcct tgatgaaaaa
     4201 cggttcgtta atttaacaag aataggtcca catatcgcaa atgttaaagc gtacattgtc
     4261 actaattgac caacgacagc ttctgataca tgtaagtcct gactcatcaa gttcataatt
     4321 cctgcaacca tcatttcaac cattcctacg ataaaaatac ttaaaatgaa tgtaaatagt
     4381 cgcatgattg aaattttaaa actcctttca acaaaatttc cgatattcat ttcccttaga
     4441 ataacgattt attaaaaaat gtgtgcaaca caattttata tataaccgtt ctattatata
     4501 atattttgct ttaattttct gtgcttaatt ggaaaattat tgaaattgtt tttcagtatt
     4561 tgaaaaatta acacaagtat gtcatttaat aattatgtat aattacttca gtttcaacaa
     4621 tgactccaaa aaagcacgca cgctttatat gtcagaacat ataactgact acaaagtatg
     4681 cgtgccttta tgaatatttt cgctgcgcta aataataaca ttactatatg tgcgcgtcac
     4741 ttcattacaa ctatagcgaa gctctaaatc acatccagca attgctatca tgactcactc
     4801 ttaacatccg tatttcgtgc tactaattga tgtcttgcat tgaaaaattg accatagctt
     4861 aaatatgtcg caatcaaagc agacataatg gtcgcagttg tatgaataaa cacgactaac
     4921 aattgaaatt taatcgcttg taaaggtggt acgccaccaa taattaagcc tgtcatcata
     4981 ccaggaatcg acacaagccc atatgtttta accgaatcaa ttgttggcac tatagctaaa
     5041 cgaatacttt cacgtattgc acctttagaa gccaatttag ttgtagctgc aagtgataat
     5101 ttagattcaa tattagtacc atcttgtacg aatgcacgat ctaaattttg gtaagctaaa
     5161 ttaattgcaa tcaagccatt atttgcaagc atgccgccga taggtataac ttcattggct
     5221 gtaaattgaa tggcccctgt cgcaatagta cctacaagcg gtaatgccgt tccaacaaag
     5281 atagccacaa aggaaatcaa aaagacatgg tgcatgacag gtgatgatcg actaatcgta
     5341 ttccatgatg cgttgacaat aatgacaaat acacataata ctaatagcca tttgtcatta
     5401 attttaaaaa tatagtgcag taagaatcct aaaatgatta attgtacgac cgcacgtagt
     5461 gtcgccacaa tcaaatcttt aataatatgt agaccttctt tataagaaat aataattgga
     5521 ataactaata gcaatgccgt cagtcctaat gctgtattac tcatgttggt tcaactcctc
     5581 tgtattagaa atttgaccat caacaatggt tatacgcttt tgaaagtgtc gcatactttg
     5641 gtcatcgctg tgggtaatcc acataattgc cacgccttga tctgctaatt taaaaatgat
     5701 attttctatt ttttctttat tattaatatc taacgcactt gttgattcat ctaatagaag
     5761 gatgtctggg gtatacatta attggcgtgc aatcgcgatc ctttgacgct cgccacctga
     5821 catatgttct attttagaac ttaattgata gtgtcctaat cctacatctt taattaattg
     5881 cttagcacgt tttttatcaa atttatcatt acgcgcaaga gacggaaaaa tcatattgtc
     5941 ttcaatcgtt tcaccaaaca aatcgctttg ttgcattaaa tagctaatgt gtcggcgtaa
     6001 tatttctggt tcgtaatcat catatggttt acctttaaag taaagcgttc cactggttgg
     6061 gctaattaaa ttacatatta atttttgaaa tgtactttta ccactacctg aaggaccaat
     6121 aattgcaatg gtttcacctt tatctacttt taaattaata tgatttagta tgactctatc
     6181 acttgcctca tacactaaat cttttatctc taacaacata tttacccctc ctcgtatcat
     6241 gagttatttc gatataatag ggtaatcctt ttcttgaata gcgtgcttac ctataagtcg
     6301 gcctagtcaa tgcttattac ccattaaaat cgctctaata aaacatatca aatatagtat
     6361 tcccattttt gaaaaattta gtgccatttc attctggaaa atttttttaa tatttatgtg
     6421 aaaattgaag tacactgcag tgacttagtc tcatctttat aaaaataaaa agcctttaac
     6481 atgacatctc atgctaacta agcatctaac ttagttcttg catgacatca tattaaagac
     6541 tttctttatt atttatggat ttgactacca catgatattt ttataagttt catcattcaa
     6601 tgaacggaca atttcagtaa caagacggac agaattttca taatcatcta cattgagtac
     6661 cgatacatta gagtgcatgt atcgtaacgt gacacctatt gtcatcgttg gaataccttc
     6721 atttgcgaca tgaatacttc ccgcatctgt acctccacct ggtgtcgtat cccattgtac
     6781 ttcgatgtta tgtttcttag ctacatcttt aatatgcttt cgcaaacctt ggtgcgcaat
     6841 acttgtagca tccatcataa taacaactgg accaccgcct agtttactat cactcgtttg
     6901 acctgacata cctggtgtat cataagcaat acccacatcg acggctatcg ccaaatctgg
     6961 tttaattgta ttcgctgcca ctttcgcacc acgtaaccca acttcttctt gcactgtggc
     7021 accactgtat aagttaatgc caatattttc atcttttaaa cgttttaata cctcaacagc
     7081 taatgcacag ccatagcgat tatcaaatgc tttcgcagtt aaatatttat catttgtaag
     7141 cacttcaaat tcactatatg gcgtaaccat attgcctact tcaacgccag cttcttcagc
     7201 ttcttccttg ctactaacac caatatctat aaacatattt ttgatttcca ttggcttttt
     7261 acgttcttca ggcgttaaga catgcggcgg tttagaaccg atgatacctc taatttcttt
     7321 gcctgaatct gttgtaatcg ttactttttg agataacatg acttgattcc accatccacc
     7381 aactggcgta aatgaaataa aaccatgttt atcaatcttt gttaccataa aaccaacttc
     7441 atccatatgg ccagaaatca taattgagta ttgaccattc tcagcatttt tctttccaaa
     7501 aatgccaccc aaattatctt caataatttg atcactgaca ggctctatat agttacgcat
     7561 tgcttcttta acttgcattt cataaccagc aatgccgttt acatctgtta aatgtttaag
     7621 taatttgact gactgattca tgttattcat cctcctgtat ttcaacttaa aactattgta
     7681 tcactaactt tcagaaaaac gttccctacc gctttatcgt tttcaggctt aataatttta
     7741 aaaactttgt gagaaatccg cttcaaaaac ctcatattta caaaaaaatt atcaaaattt
     7801 aaatttttga ataacatcat tttttagtaa atgttataat ttctttataa tacaaatatc
     7861 gaaagaaggc gactgacttt gaaaaaatta tgttcattaa ttgtagtagc attagtttgt
     7921 gtcattgcat tatcagcttg tggtaaagaa caaaccaaaa catatgaagg tgatgttagt
     7981 ggtaaacatg tactaacttc tatcacatat aaagatgata aagttttaaa acagtctaca
     8041 atcaacacaa ttaaatatga cgatctaggt atggataaag acgaagccaa aaagttattc
     8101 gccaaatctg aaagtatttt caaagacctt aaaggcgtaa aatacaaagt agactataaa
     8161 gataaaaaag caattgaaca cttagacata gattacacag aagttgacat gaaaaaatta
     8221 aataaacgtc ttggtgtttc aactaaagaa aataaagata ttagttttga aaaacttgaa
     8281 aagcaattaa agcacagagg cttaaaagaa aaagataaaa tggacgacaa atagtttata
     8341 acataaaaat gccctcagat aagactaagg ttacaaacct taattcatat tctgagggct
     8401 ttaatatttg aaattcttgt gtgaccaaca tccactacta atataaaatt atttgcagta
     8461 acgctaaaat ccgctgcttt cattttcccg aaataaataa gttaactaat gagttttaat
     8521 ttataatcat gtatcgtttg caattcacca tcgacttttc gatatacaat atgatcagca
     8581 gtaatttctg tagggcttga tacgccaaca gctgctgcaa tattgaataa gccttcatgc
     8641 aaacttgtta catagtttgt gacacgatat tgcttttctc caacaatcaa tgctttttct
     8701 tttttcgcat ctgtcgttgc aacacctaca ggacatgtat tcatatgaca ttgttgactc
     8761 ataatacaac cgacactaat catcatgcca cgtgcaatat taacaaaatc tgcacctaaa
     8821 cctagtgcaa tcgcaatttt atctggtgtc actaacttac cagatgccgc taatttcact
     8881 ttatctcgaa taccatattt ttctaacatg ccagacacaa taggtagagc tgtaaatagc
     8941 ggtaagccaa caccatcttg taattcttgg aatgttgcac cagtaccacc ttcaccaccg
     9001 tcaatcgtaa taaagcttgg atacttatct aattccacca tcgtacgtac aagtgtttca
     9061 atttctgaaa ctttgcttac tacaattttg aatcctactg gtttttgacc taattgctgc
     9121 aactgatcga cgaaacgaat caaatcttca gcattatgaa taaattcgta acggttaggt
     9181 gaattgattg ttttataagg ttcaacattt cggattttag caatttcttc gtttaccttt
     9241 tcagcttcca tatgaccacc acgagtctta gcaccttgtg ccaacttcag ctcaaatgcg
     9301 cgaacgttag ttaactgtgc gacctcttta aataaacctt cactaaaatt accttcctta
     9361 tcacgaacac caaataaacc gggaccaatt tggaaaatta tatccccatt accttttaaa
     9421 tgatattctg ataagccacc ttcacctgta ttcatccaag tgcccgcttt agctagacct
     9481 ttagataaag ctgtaatggc attttttcct aaggctccat aactcatacc cgattgtcct
     9541 acgatacgtt ttaaaataaa cggatgtttt aaatgttcac ctaattttat tgcatgatca
     9601 tcacttaagt aatacggatc aatctttgtc ggtacacgat attcttcacg actaaataaa
     9661 cgctcattcg cgattttata aatgaatgtt gataacaatg ttgtattatc tactgaaatc
     9721 tcattacgtt gcatcggaaa cattgtgttc tgtatgtaaa agccgtcttg ataatcttta
     9781 gtagtaccga agctggtcat acgagagtta tattttccag ccaaaacgat atttttataa
     9841 tcattacgtg aaaaaggttt cccttcatta tccccagaaa ataaatactg acgtaattcc
     9901 ggtcccattt tttctgaaat atatctaata cgtgctagta aaggataatt ccttaataca
     9961 ctatgttgtg attgtctttt atctttaatt aaccaaataa gcccgataac catgaccgta
    10021 agcaggaagc ctataatgat aatattaact ataaattgca tgactgtaag aaacgtcatt
    10081 acaatccctc ccccaaaaaa tttcaattca atatttatga tacaccttac aaaataaaac
    10141 acaatggaag cgcttcattt tataaaacaa ttttatgata tgtttttcat tttaaatttt
    10201 aatgtataaa acatacaata caaagtaata tgtgctaaag tatctatata atacaactat
    10261 ttaagaggta tactatgtca aatacaaata aacattacat agaagaagaa tacgctaccg
    10321 aacaatcgcg ttttttcaaa cgtgatattg gatttatttt cctttatata tttttggtta
    10381 acatcttgcc aatcattatc gtttttttaa cttttgggtt agtagctgta ttcactcaac
    10441 aagccccatg gggtttctct tttcctattt caatgaacat tagctattta ctcgcagtta
    10501 ttatttcact agctattttt cgtcggatgc atgaagatgc atttgttcca atgatacgta
    10561 tgcaaatcgt tgccagtgct aaatataaat ggcatattct tatatcattt acattaagta
    10621 tcgtactctt tttactttta aatcatttcg ctattttcgc actaaatgta gattccttta
    10681 ctgtctttga catttacccg ctcattagag aatatggttc catcagttta atagctatat
    10741 tttcgttaac cattatatta aatactttga tttgtcaact tattttccgt catattttaa
    10801 ttcaagaatt aagcagatta atacctttaa agattacaat agtattatcc gttatccttg
    10861 aaacactgta ctattatcca tacattaata catggtggga attcattcca gcattgatac
    10921 tagctagtag tgcgacttac ttatatttaa aatcaaaccg taattttatg gtgagctatt
    10981 tctatcaatt agcagttata ctcgttttgc atattttcat gtaaattttt tcagagtatt
    11041 atccattatc catacacatc aaaaagcaca cttaaaagtc agtatcacaa ctcactttta
    11101 agcgtgcttt atttagatat actgtcgttc taaaaacgag ccatatagaa tgtttcatgt
    11161 cctagcatga agcacatgaa tcgccatgct caatatacct tttaaaattt aaatattcat
    11221 taacttaata tgctttaatt cacttcatta aagactattt gctgggcgtg gtgttggttt
    11281 tttcaataaa ctcataatca ttattacaat gactaacata ccacaaatta gatacatcca
    11341 tttaaatcct actaaatcgg aaataggtcc catcaatgcg ccacctaacg atatacctaa
    11401 gtctgcacag gcaataaata aacctaacaa catattacga cctacttttg gtaagacgaa
    11461 acttaagtat gatgttaatg ttgggtacac cattgcttgt gtcattccta ttaatattgc
    11521 actgccatag aaaataattg cacctacttg cggaccaaat gctactacaa atgacgcgat
    11581 tactaataat gatagtacag ataccatata tttaggatgc cacataccat ctgacggaat
    11641 gtatttcctt aagtaaaatc ttgccacaac aactgcgatg gcctgtattg tcaaaaagat
    11701 tcccgcattc gcgaatccta aactcactgt gtataacggt acaaatgtac taactgcacc
    11761 aaatacaatc gatgcaacaa tcatgataat accactgttc aacaactctt tatttttgaa
    11821 aaattgcgca aaaacagtta cagcgttaaa cggcattttt tcaatcttat ctgacgtatc
    11881 gggttcctgt tcagcaaagg tcacgcgata gccaaagaat gttgttgtta atgcgataaa
    11941 gataatgaca attgcaaata gtgaaatatt atttgcattc caaataccta cggcaactaa
    12001 tggtccgatt aagtttggaa tcgttgaaaa tagcgagtac aatgatacac cttcagaacg
    12061 atgttcctcc ggtaatgcat caataatacc tagctgtaaa gacatagaaa agaatgccgt
    12121 acacacacct tgcataacac gtgctacgaa ataaccttct aagccagtaa aaccataaat
    12181 aattaaagct atggcattga taatcaaaat aattcttaat actttaatgg gaccgacacg
    12241 agcaataatt tgtcctgccc atggtcgaaa taccattgct gttaacatgt atgcccccat
    12301 aacgataccg ataaccgtat tcgttgcgcc taaatcatgc cctcgtaaag gtataaacac
    12361 gtttaagatt gcattggcac taaagaacat caatgttaat atatataatc tcaaaaaggg
    12421 ccaagccatt gcacctttca ttatctcact ccttagaatg atagttgttt ttctattaat
    12481 tctcttgtat aagcattgtc acttttatgc aatgcgcttg tcggaatttg ttcttctatt
    12541 tttccgtttt taaaaataat taattgatca cataaatacg tggcagcttg aatatcatgt
    12601 gtgataaaaa tataactcaa ctgacgcgtt tcacgtaaat gaatcaataa atctaatatt
    12661 tgtgtttgaa ttgacatgtc gagtgaacta atggcttcat caaacaaaat atatttaggg
    12721 ttaatacata ttgcgcgcgc aatcgcaaca cgctgtgcct ctccacctga taacatatta
    12781 ggatatttat ccatgtatgc cttagataga ccaacttctt ccaacaatgt aattgcttgg
    12841 acttccataa catctttagg ttgtccatca cattgacaca tcacttcaaa taagatttct
    12901 ctaacagtct gaaatgggtg taatgatgac gtataatctt gaaatacagc accaatttga
    12961 tgacgtctca ctttcttctt atgcatcggt tgatcattta aggttacaca acctttatct
    13021 ggtttctcaa tacctaatat catacgactc aacgtcgatt taccgctacc actttctccg
    13081 ataatcgcaa tcgtcgcacc gattggacac tcaaatgaca cacctttcac gataggtgtt
    13141 cgacgacgct taaaaacatg tgcgctttga tatgactttt caacatcttt aattttaatc
    13201 atgtacatca cccctcatca catgtttaaa atgatcatta atctttttct tagtggataa
    13261 taaatacttc gtataaacat gttctggatg atgcaagact gattcacgcg taccatgctc
    13321 aatcagttga ccatttttca tcacaacaac acggtctgca atcttgttaa taaccgttaa
    13381 atcatgtgaa atgaaaatca tcgcacagtc aaagtgtttt tttatatcta taaatgcttc
    13441 cagtacatca tattgtgtaa ttgtatctaa agccgttgtc ggctcatcag caatgattaa
    13501 ctttggtttc aaagctaacg ctaaagcaat catcaatcgc tgtaacattc ctcctgataa
    13561 catgtaaggg tatgatttta atatacgttt aggatctttc aaacttaaat aatccatata
    13621 ttcaatcaat gtcttttcaa tttcttgtgt agacattgac gtatgtactt tcatagtctc
    13681 aaacatttgt ttaccgacag tagttgatgg atcaaaggca cgactacctt gttgcatgac
    13741 catcgcaata tctttaccac ggtatttttt caattgtgtt tccgataacg acaacattga
    13801 cttaccatca aaggttatat cacctgttac gcgtaatcgt tctggattta aacctacaat
    13861 cgatttgcat gtaattgatt taccgctacc actttctcca ataacgccta aagtttcacc
    13921 cttagttaat gtaaaattca catcactcac gagtggttga tctgtccagg tatctgtaat
    13981 cgtcaaatgc ttaactgtta acaatgtcat gattgcacca ctcctttttt cacagaacga
    14041 agtttatctt tagaagagat gcggggatca atggcaattt gtaaagcatc ggataagaag
    14101 ttaaatgcca tgactataat cactatggca atacctggcg caaacatcat ttcaggatgt
    14161 gtaaacatca cttttctagc ttcgttaagc atcatgcccc actctgcagt aggcgctttg
    14221 acacctaatc ctaaaaatga aaagccagat atttgcaaga tcattgaaca catcgaacta
    14281 ctagagatga tagcaatatc tgctaatgtt aacggcataa tatgtttgtg aataattttc
    14341 atatcattca taccgattgt tttagcaaat cttacatggt cagaagcagt gtactgcata
    14401 acacttgtac gtataacgcg acagaaccat gcccaacgcg tcaaaataaa tgccatgata
    14461 atattttcgg cacccatacc aaacaatgca attaatgcta acgttacaac ataacttggg
    14521 aatgccaaca taacatcaca cgcacgcatg attaaggcgt caacaaaccc ttggaaatat
    14581 cctgataaga atcctaaaat agatccaata agtacagaaa caaatagtgc aacaaagaca
    14641 tataacaaac ttggtctaat cgcataaatt aacctagtta aaatatctct acctaaatgg
    14701 tcagtaccta gtagatgttg aaaactcatg ccagcaaatt tgtttgctgt atcgatatgg
    14761 ttaggatcat aaaatgtcac aagtggtgct gctaatccta aaaagacata taatacaata
    14821 atgcctaaag caatcactgc acctttatct tgtaataatc gttttaaaat tatcatcgtg
    14881 cgccctccct taatcttgga tttaatagcg cattaatgat atctgctaat gtattaaata
    14941 caataaataa taccgctaca attaatacat atgcttgaat gactggaaaa tcatgttcaa
    15001 gtattgcttt taaacttaat tgacctaatc caggccatgc aaagatatac tcgataacaa
    15061 ctagtccacc cattatcatt ggtatagaca tacaaaagat tgataccgca acttgtaaag
    15121 cattacgcaa cacatgcaac attaatgtga tagatttcac accgcttgct cttaaataaa
    15181 gtacataatc ttcatttaat tgttccacca tcgagcgtct aacatttcta aagtaaatac
    15241 cagcataggc aatcgtaata acgatcac
//
LOCUS       DAMZPP010000065        15206 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMZPP010000065 DAMZPP010000000
VERSION     DAMZPP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15206
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.065"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..500)
                     /locus_tag="POH73_001514"
     CDS             complement(<1..500)
                     /locus_tag="POH73_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDI6887465.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEA"
     gene            851..2056
                     /locus_tag="POH73_001515"
     CDS             851..2056
                     /locus_tag="POH73_001515"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDI6887466.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMRAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            2046..3425
                     /gene="argH"
                     /locus_tag="POH73_001516"
     CDS             2046..3425
                     /gene="argH"
                     /locus_tag="POH73_001516"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDI6887467.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLSTYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            3667..4596
                     /locus_tag="POH73_001517"
     CDS             3667..4596
                     /locus_tag="POH73_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI6887468.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNHIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DENVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNKNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4991..6235)
                     /locus_tag="POH73_001518"
     CDS             complement(4991..6235)
                     /locus_tag="POH73_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HDI6887469.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(6344..7534)
                     /locus_tag="POH73_001519"
     CDS             complement(6344..7534)
                     /locus_tag="POH73_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDI6887470.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINKNTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFARDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            complement(7842..8969)
                     /locus_tag="POH73_001520"
     CDS             complement(7842..8969)
                     /locus_tag="POH73_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDI6887471.1"
                     /translation="MKSKYEPLFDKVVLPNGVELRNRFVLAPLTHISSNDDGTISDIE
                     LPYIEKRSQDVGITSNAASNVSDVGKAFPGQPSIAHDSDIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPIAVIEEVLKSKEVYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATAREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADDALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(9331..9708)
                     /locus_tag="POH73_001521"
     CDS             complement(9331..9708)
                     /locus_tag="POH73_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDI6887472.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(10123..10716)
                     /locus_tag="POH73_001522"
     CDS             complement(10123..10716)
                     /locus_tag="POH73_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDI6887473.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            10780..11163
                     /locus_tag="POH73_001523"
     CDS             10780..11163
                     /locus_tag="POH73_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HDI6887474.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHERKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            11294..13699
                     /gene="mnhA1"
                     /locus_tag="POH73_001524"
     CDS             11294..13699
                     /gene="mnhA1"
                     /locus_tag="POH73_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HDI6887475.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWIWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASKANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTVFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            13692..14120
                     /gene="mnhB1"
                     /locus_tag="POH73_001525"
     CDS             13692..14120
                     /gene="mnhB1"
                     /locus_tag="POH73_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HDI6887476.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            14120..14461
                     /gene="mnhC1"
                     /locus_tag="POH73_001526"
     CDS             14120..14461
                     /gene="mnhC1"
                     /locus_tag="POH73_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HDI6887477.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            14454..>15206
                     /gene="mnhD1"
                     /locus_tag="POH73_001527"
     CDS             14454..>15206
                     /gene="mnhD1"
                     /locus_tag="POH73_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HDI6887478.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPLRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGAL"
BASE COUNT         4858 a         2683 c         2400 g         5265 t
ORIGIN      
        1 tgtgcttctt ctttaccgta tctttcttca actaattgtt tgaacaatct aaatgcaact
       61 gctggttctg tagttgtacc agatttagaa ataacgttta cagagaaatc tttgtctgct
      121 aaataatcaa ctaactcttt agtgtatgtt gatgataagt gattaccaac aaatacaatt
      181 tcagggtatt cattgctgtt tctaaatgat gacgttaaca tttcgattgc tgcacgtgca
      241 cctaagtaag aaccaccaat accgatgact actaaaacat cagaattttc tttaatgcgt
      301 tttgatgctt caacaattct tgaaaattct tctttgtcgt aatcaactgg taaatcaacc
      361 cagcctaaga agtcactacc tgcaccagta ccttcatgaa ttgttttgtg aattgattta
      421 acaatttctt gttgttgttt taattcgtgt tcaccgaaaa attctaacgt tttactaaaa
      481 tctaattgaa tatgagtcat ctataaaagc ctcctgtttc atttaatatc ttaattttac
      541 taagtttacg tttgcgattc aattcacaag tttgtactta atcaataaaa ttgatattta
      601 tgtgacgttt cacacctatt tttcaacaaa catttcaaac cggcgtcaaa cactcaaaca
      661 ttttttatac aatttgcatg ttttttaaaa cacttttaaa cattgataaa taagtattta
      721 tacttacaac tctaactacc tctatcatat tttatgaata aatatgtaaa ataatggttt
      781 tattttcatt aaaagtttgc tatcatgtgt tcatacgaaa tattcagata actacaatga
      841 ggtgaatttt atgaaagaga aaattgtttt agcatattca ggaggactag atacaagtgt
      901 ggccgttcaa tggcttatcg acaaaggata cgacgttgta gcttgttgcc tagatgttgg
      961 tgaaggtaaa gatttagaca tcgtttataa aaaagcttta gatatgcgag ctgttgaatg
     1021 tcatattatt gatgcaacaa aagaatttag tgatgagtat gtaagttatg caatcaaagg
     1081 aaatttaatg tatgaaaatg catatccatt agtttctgct ttatcaagac cattaatcgc
     1141 taaaaaatta gtagagattg ctgagaaaac aaattcagta ggtattgcac atggttgtac
     1201 tggtaaaggg aatgaccaag tacgtttcga agttgccatt aaagcattaa acccatcatt
     1261 gaaagcattc gcacctgtac gtgagtgggc atggagtcgt gaagaagaaa ttgattatgc
     1321 aattaaacat aatatccctg tatcaattaa ccatgattca ccttattcta tcgatcaaaa
     1381 cctatggggc agagcgaatg aatgtggtat tttagaagat ccttatgctg caccaccaga
     1441 ggatgcgttc gatctaacaa atgctttaga agaaacacca gatactgctg atgaaatcat
     1501 tttaacgttt gataaaggca tcccagttca aattgatggc aaaacatatg aattagacga
     1561 tttaatttta acgttgaatg cattagctgg taagcatggt atcggaagaa ttgaccatgt
     1621 agaaaataga cttgtaggta tcaaatcaag agaaatttat gaagcacctg ctgcagaagt
     1681 tattttaaaa gcacataaag cattagaaac gattacgtta acgaaagatg ttgcacactt
     1741 taaaccaatc attgagaagc aatttgctga acaactatac aatggacttt ggttctcacc
     1801 tttaactgat agcttgaaat tatttattga tagtactcag caatacgtaa gtggtgatgt
     1861 cagaattaaa ttattcaaag gtaatgctat cgtgaatggt agaaaatcac cttacacatt
     1921 atatgatgaa aaattagcaa cttatacaaa agaagatgca tttaatcaag acgctgctgt
     1981 tggctttatc gatatctatg gtttacctac tcaagtaaat gcgatgttac atggaggcta
     2041 tagcaatgag caataaagct tggggcggta gatttgaagt acaacctgaa gagtgggttg
     2101 acgactttaa cgcatccatt acttttgatc aaacgctcat agatcaagat atcgaaggca
     2161 gcattgcgca tgcaactatg cttgcgaatc aaggcattat tagtcaacaa gacagcgaac
     2221 aaattataca aggactaaaa tctattcaac atgattatca tcaagatcaa attcaattta
     2281 gtgcatcatt agaagatatt catttaaata ttgaacatga attaattaaa cgtattggtg
     2341 atgctggtgg taagttgcat actggacgca gtagaaacga tcaagttgca acagacatgc
     2401 acttgtacac taagaaacaa gtgcaagata tcatcgcatt aattaagtcg ttacaaagtg
     2461 taattgtaga catcgcttcc aataatgttg atacaattat gcctggttat actcatttac
     2521 agcgtgcaca gccaatttca tttgcacatc atattatgac ttatttttgg atgttacaac
     2581 gagaccaaca acgatttgaa gatagtttaa aacgaatcga tattaatcct ttaggtgcag
     2641 cagccttaag tggtaccaca taccctatcg atagacacga gacaacagca ttgttgaact
     2701 ttggcagcct ctatgagaat agcctagatg ctgttagtga cagagactat attattgaaa
     2761 cattgcataa tatttcttta acgatggttc acttatcacg ttttgcagag gaaattattt
     2821 tctggtccac agacgaagct aaattcatta cattatcaga tgcattttct actggctcat
     2881 ctattatgcc acaaaagaaa aatcctgata tggcagaatt aattagaggt aaagttggtc
     2941 gaacgactgg tcatttaatg agcatgctta tgactttaaa aggattacct ctagcatata
     3001 acaaagatat gcaggaagat aaagaaggtt tattcgatgc tgtccataca attaaaggtt
     3061 ctttacgtat tttcgaaggt atgattcaaa cgatgaccat taataaagaa cgactcaatc
     3121 aaactgttaa agaagatttt tcaaatgcaa cggaactagc agattattta gtaactaaaa
     3181 atattccatt tagaactgca catgaaattg taggaaaaat cgtcttagaa tgtatacaac
     3241 aaggtcatta tttattagat gttcctttat caacatatca acaacatcat tctagtatcg
     3301 atgccgatat ttacgattat ttgcagcctg aaaattgctt aaaacgacgt caaagttacg
     3361 gttctacggg tcaatcatcg gtcaaacaac aacttgatgt tgctaaacaa ttactatcac
     3421 aataaatacg ttaatctacc tacccacaat gtctatgaaa attacattgt gggtatttta
     3481 atgctctctt cgtcttgttg aacatcacat ttttaagatt cctaaaatgt ttgataattc
     3541 ttttaaattt atattacaga aatgttataa attgtaaaag aaatgtgtaa agcgttttca
     3601 caagcaggtt tttgtagtat tttaaaattg ttagactaca aataaagaga tgaaaggata
     3661 aagactatga ctaactcttc gaaaagcttc actaaattta tggctgcttc tgctgttttt
     3721 actatgggat ttttatcagt acctactgct ggcgctgaac aaactaatca tattgcaaat
     3781 aaacctcagg ctattcaatg gcatacaaat ttaacgaatg agcgattcac tactatcgca
     3841 catcgtggcg caagtggcta tgcacccgag catacgtttc aagcatatga taagagtcat
     3901 aatgagttaa aagcatcgta tatcgaaatt gatttacaac gtaccaaaga tggccattta
     3961 gttgctatgc atgatgaaaa tgttaaccgt acaacaaatg gacacggtaa agttgaggat
     4021 tatacccttg atgaattaaa acagttagat gcaggaagtt ggtttaataa aaaatatcca
     4081 aaatacgcaa gagcaagtta taaaaatgct aaagtaccca ctttagatga aattttagaa
     4141 cgttatggtc caaatgcaaa ctattatatt gaaacaaagt cacctgatgt atacccagga
     4201 atggaagaac aattattagc ttcattgaaa aagcatcacc ttttaaataa aaataaatta
     4261 aaaaatggac atgtaatgat tcaatcattt tctgacgaaa gtttaaagaa aattcatcgt
     4321 caaaataagc atgtgccatt agtaaaatta gttgataaag gtgaactaca acaatttaac
     4381 gaccaacgct taaaagagat acgctcttat gcgattggat taggtcctga ttatacagat
     4441 ttaactgaac aaaataccca tcatttaaaa gacttaggat ttatagtaca tccttataca
     4501 gtgaatgaaa aagctgatat gttacgatta aataaatacg gcgttgatgg tgtctttaca
     4561 aatttcgctg ataaatataa agaagtcatt aaatagtatt gttaaactag aaaacataaa
     4621 tacaaaaata tagctattac tataaaaaac agcagtaaga tatttccaaa ttgaaattat
     4681 cctactgctg tctttttgct caactatata tagttgagcg tttaattaga aattctctgt
     4741 gttgggggcc caccccaact tgcacattat tgtaagctgg ctttccacca gcttctgtgt
     4801 tggggccccg ccaacttgca cattattgta agctgacttt tcgccagctt ctgtgctggg
     4861 gccccggcta tttttgaaat aagcttgtta caagtgtatt tttattcagt caactactgc
     4921 taaaatataa ttaacgagct tagggctttg ttttctgtcc caagctcgtt aaatcacata
     4981 tgataattaa ttatgcccaa ccacgatatc tagctgcttc tgctgtacgt ttaataccta
     5041 tgatatatgc tgcaagtctc atatctattt ttcgattttg agacaattcg taaatcgtat
     5101 caaatgccgc ttctaatttt tcacgtagct tttcattaac ttcttcttca gaccaataat
     5161 aaccttgatt attttgtacc cattcgaagt atgaaaccgt tacaccacca gcacttgcta
     5221 atacgtctgg aactaataat ataccacgtt cagttaaaat acgtgttgcc tctggtgttg
     5281 taggtccatt agcagcttca acaacgatgc tagctttaat atcatgtgca ttgtcttctg
     5341 taatttggtt tgaaatagcc gctggtacta aaatgtcaca atctaattca aacaattctt
     5401 tatttgagat tgtttcttca aataaatttg ttaccgtacc aaaactatca cgacggtcta
     5461 ataaataatc tatatctaag ccatttggat cgtgtaatgc accgtaagca tcagagatac
     5521 ctacaatttt tgcacctaaa tcatataaga atttagctaa gaaacttccg gcattaccga
     5581 aaccttgaat aacaaccttg gcaccttcaa tttgcatatt acgacgtttt gcagcttgtt
     5641 caattgcaat aactacacct agtgcagttg atctatcgcg tccatgagaa ccacccaata
     5701 caattggttt acctgtgatg aaacctggtg aattaaattt atctaatgca ctatattcat
     5761 ccatcatcca agccataatt tgtgagtttg taaatacatc tggtgctgga atatctttgt
     5821 tcggacctac gaattgtgaa attgctctta catatccgcg tgataaacgt tcaacttcat
     5881 gaatgctcat ttgacgtgga tcacaaacga taccaccctt accaccaccg tatggtaagt
     5941 taacaatgcc acatttcaaa gtcatccaca ttgataatgc ttttacttct tcttcatcaa
     6001 catctgggtg gaaacgcacg cccccttttg ttggtccaac agcatcatta tgttgcgcac
     6061 ggtaacctgt gaatgtttta acagtgccat catccattcg tacagggata cgcacttgta
     6121 acattcttaa aggttcttta attaaatcgt acattccttc gtcaaatccc aatttatgca
     6181 atgcttcttt aataattcct tgagtagaag ttactaaatt attgttctca gtcatgatcc
     6241 ttttcgcctc ttctttacct aatgatttcg ctttcaaaca tattgtaaca taacgtattc
     6301 ctttttaaag cccttacaaa ctgattgtta caactttttg acattattga aatacatgtc
     6361 ttattttttc aagtgcaagg tccaattctt ctttagtaat aattaatggt ggtgcaaaac
     6421 gaatgacagt atcatgcgtt tctttacata ataagccttc ttcttttaat gcttcacagt
     6481 atggtctagc actttcatta agttccacac ctataaacaa accacgtcca cggacttctt
     6541 taattgatgg atgatcaatt tgctttaatt gttctttaaa ataatctcct aattctaaag
     6601 agcgacctgg taaatcctca tcaacgataa catctaatgc agcaattgat gctgcacaag
     6661 caagtggatt accaccaaat gttgaaccat gtgagccagg tgtaaagaca tctaatactt
     6721 ctttatctgc taatacaaca gaaattggga agacaccacc acctagtgct ttacctaaaa
     6781 tatagacatc aggttttaca ttatcccaat ccctagcaaa taatttaccc gaacgaccta
     6841 atcctgcttg gatttcgtca gcaataaata agacattatg ttcatcacat aattctctaa
     6901 ttgctttcaa atatccttct ggtggtatat ttatacccgc ttcaccttga attggttcta
     6961 ctaaaactgc tgcagtattt ttattaattg cagctttcaa tgcatctaca tcaccaaaat
     7021 caacttttct aaacccatct aataatggac cataaccacg ttggtattct gcttctgatg
     7081 ataatgaaac tggcgccatt gttcgaccat ggaagttacc attaaatgca atgatttctg
     7141 ctttatttgg ctcaattcct ttaacatcgt atgcccagcg tcgtgctgct ttcaaagctg
     7201 tttctactgc ttcagcacct gtattcattg gtaaagcttt atctttacct gccagtttac
     7261 aaattttttc gtaccattct cctaagttat cactatgaaa agcacgtgaa actagagtca
     7321 ctttatcagc ttgatctttt aatgcttgaa taattttcgg atgtctatga ccttggttaa
     7381 cagcggaata tgcagataac atatccatat atttattgcc ttcaggatct ttaacccata
     7441 ccccttcagc ttctgaaatg acaattggca atggtaaata attatgtgct ccgtaatgat
     7501 ttgttaactc aataattttt tcagatttag tcatcatatc tcctcttttc atcatttata
     7561 actattatac atgaaacatt atccaaataa ttacattagt tttcaaagca gatacttttt
     7621 cacccgaaaa gatgaattaa tcactaagtt tcattaaatt tgtctatttt gaaaaccctt
     7681 acatttataa tgacataatt acttaaatga ttacaagcaa aagaattgat aattttacac
     7741 ttaatcaaaa gtatatttta ctaagaatat ttttatttat aaatattgaa aaccactaac
     7801 aaattgcata cacaatatca ttagtggtaa cagtcaaaca cttatttatc tttacgtggt
     7861 aatggataga acccttcatt aaattgatgc cataaattag gagttaagtg atgattatct
     7921 tctctctctg gatcaaagta attaataatt tcatcttccc gtccatcttt aattttttca
     7981 acaaattgat aatctagtag tagctctcta ccaatggcta ctaagtcaac accaacattt
     8041 tcaactgcat ctaaagcgtc gtcagctgtg aaaattgaac caataccgat aagtggcata
     8101 cgaccattta tccatttgtg aattaaaggc agtctttctt gtccagcgta tttaccttca
     8161 cgtgccgttg catgcgtatc catcattgaa acatgaatat agtcgattgg catatggcta
     8221 attttattaa cgagttcctc tgtaatttcc attgtgattc ctggagactc cgcttcctct
     8281 ggagataatc tgtatccaac tataaagtct ttattaccat acacttcttt cgatttaagc
     8341 acttcttcaa tcacagcgat cgggaattta tattgatttg cccatacatc atttcttcta
     8401 ttatagtatg gtgatacaaa ttgatgaatt aagtaatgat tcgcgccatg aatttcaaca
     8461 ccatcaaacc ctgcttcaat tgcacgtcgc gttgcttcac caaaatcctt gattgcttgt
     8521 tcaatctctt cattcgtcat ttctctagca ctatgttctt gtttctgacc gaaacttttt
     8581 aaagaaattg gacttggtgc tacgacgtct ccatcaggtg ttaattcagg caatgcttgt
     8641 gcaccgccat gatgtatttg tacgagtgct ttggcaccgt ttttcttcat tgctgtagct
     8701 aatcgtttta gtccttcaat atcactgtca tgcgcgattg acggctgtcc tggaaatgct
     8761 tttccgacat cactcacatt actcgcagca ttacttgtaa taccaacatc ttgtgaacgc
     8821 ttttcaatat aaggaagttc tatatctgaa atagtaccat catcatttga agaaatatgt
     8881 gttaaagggg ctaacacaaa tcgatttctc aactctactc catttggtaa tactacttta
     8941 tcaaacaatg gttcgtattt acttttcatt acttaattcc tccttaaaat tattgagttg
     9001 actataacat gtaatttttt cattctccaa ttatctgttt atatagttgc aattcgttta
     9061 atactttaag ttgtagttct catttctaaa ataaattaaa aaaacaaatc cacatacatc
     9121 attgcaaatc aacgattcta tctaataaag ataatggata tgtcacctta aaccatttgt
     9181 acgtgtaaat agaatcaatc atattacgtt aacgcagaca aaaagagatt agtatctaat
     9241 ataatttaat agatactaat ctcttttata gtctaagaaa ctttcatttc agaccctttg
     9301 tatgattttc ggtacgattt atctgttcct ttagtcgttt cgaattgctc gctttgactg
     9361 ttttatccag attggtaagc gttctttaag tgtttgaaac ccatattttt ctgtttctct
     9421 gatttcatct aatactgatt gtttttcctt cttacgctca taatttttga agtaatcatt
     9481 atcctttaat gatagattga gctttccttc atcatctata gacaaaattt tagctttaac
     9541 aatttggcct tctgatagaa atttcttcaa attatgaacg tagtcatcca taatttctga
     9601 tatatgaatc agtccttcag tatgattagg ggtctcaaca aacgcaccgt atggttgaat
     9661 accagttaca cgcaccttga tatgttggcc aattttgtag ttattcaact taataacccc
     9721 ttatttcgtt tacttaacac tattatcata gcacaaatcg aaatggtgga ctaattaaaa
     9781 catcaaatca tacgaatatt attttataaa tgaataagaa tactacaaac ttggatttac
     9841 aaataaaatc agcaacaaat tcctatctaa atcaatatga tatccagcta ctttgttttc
     9901 aatttattaa ctatttgaat agcaattctc tgtaaaacat aaaaagcaac ctcaactatt
     9961 atctctagaa tacacatcta tttaaaaact gtgctttctt gataaatata ttgagattgc
    10021 tcatcatatt agtttatttg ttttcgttgg aatatatact aatccattca taaattagct
    10081 ttatccgagt ttaaaatgtt ggcagttaat tatgtttaga tattattctt caacatcaat
    10141 agattcaatt acaacatcat gaagtggttt gtcttgtggt cccacttttg tatttgcaat
    10201 atcttctaaa gtagtttcac catcaatgat ttgaccgaat actgtatgtt tttgatctaa
    10261 ccatggtgta ccaccctttt cgccatatgc atcaacgatt ggttgaggcc agccaccatc
    10321 tgcaagttga cttaacatat tttgaggtac ttctttcatt tgaacaatga aaaattgtga
    10381 accatttgta ttaggtcctg agttagccat tgataatgcg ccatataagt taaatgcatt
    10441 taacgaaaat tcatcttcaa aagcaccgcc ataaatactt tcgccaccca taccagtagc
    10501 tgttggatcg ccaccttgaa tcatgaagtc attaatgaca cggtggaatg ttattccatc
    10561 ataataacca ttttttgcat gtgtcacaaa attttcaact gtttttggtg caatatttgg
    10621 aaataatttg aatgtcatgt cacctttatt tgtgtgcata accactttaa tttcaccttg
    10681 ttgtacttct ttgtttaact gtggatagtt agccatttat attctccatt catgttatga
    10741 tacatatata ttaacatact tttaagggag gaaaatagca tgaagctata tcgattttca
    10801 cacaaaacag gtagctatgg tgtcacgatt gttgaagaaa atggagacga tgtccttgtt
    10861 aaagttgaac aagttatcaa acacccgaaa caaggtgatt tacataaccc tactgaaact
    10921 gaaggtgtat ttttccatga acgcaaagca ttaagtcatt ttgaaaaacg ctatacgaaa
    10981 cgttcacaat tacgcgactt caatgttgat gtattgcgct atgaagattc acttcaacaa
    11041 gcaattacag atttagaaac taaacttaaa gctcagcaaa caaaacatgc tgaaatgtct
    11101 ttagctagtt tagcacgctt aaaagaagat tattcacttc aatacaaaga aaattttaaa
    11161 taaaaaatga cgtgtttctt cttatttaaa tacttctgaa aataaagcac aacaaggagt
    11221 gtgtacgttt ttcagaacta tatcattcat gtataatgaa taacataaat attttttaaa
    11281 ggaggacaac atagtgagtt tgttacatat tgcggtgata ttaccgttaa tattcgcact
    11341 cattattcct atcctgtata gattttttaa acgtatacat ttaggatggt tcgtattacc
    11401 tgtccctatc gtcatattta tttatatgct tacattaatt aaaacgacga tgtcaggtaa
    11461 tactgtaatg aaaaccttaa attggatgcc tcattttggt atgaactttg atttatatct
    11521 tgatggctta ggtttactct ttagtttgct tatttcagga atcggcagtt tagtagtatt
    11581 atattcaatc ggctacttaa gtaagtcaga acaactaggg aatttttatt gttatttatt
    11641 actatttatg ggtgcaatgt taggtgtcgt actttcagat aatgttatta ttctatattt
    11701 attctgggag ctaacgtcat tttctagttt tttacttatt tcattctggc gcgaacgaca
    11761 agcatctata tatggtgctc aaaaatcgct tataatcaca gtatttggtg gtctatcatt
    11821 attaggtggt atcatcttac tcgctatacc aacacaatca tttagcattc agtacatgat
    11881 tcaacatgct tctgaaattc aaaatagccc attcttcatt tttgcgatga ttttaatcat
    11941 gattggtgct tttacaaaat ctgctcagtt tccattttat atttggctac cagatgcaat
    12001 ggaagcacct acaccagtca gtgcttatct tcattcagca acaatggtta aagcagggtt
    12061 atatttaatc gctcgaatga caccaatctt cgcagcatcg caaggttgga tatggactgt
    12121 cacacttgtt ggtttaatta cgttattctg ggcttcgcta aacgcaacaa aacaacaaga
    12181 cttgaaaggt atactggcat tttcaacagt atcccaatta ggtatgatta tggcgatgct
    12241 tggaatcggt gccattagtt atcactatca aggcgatgat agcaaaatat atgctgctgc
    12301 attcacggct gcaatattcc atttaatcaa ccatgcaaca tttaaaggtg ccctatttat
    12361 gattactggt gcagtggatc attctactgg tacacgtgat gttaagaaat taggtggctt
    12421 actaacaatt atgcctattt catttacaat cactgttatt acagcattaa gtatggctgg
    12481 tgtaccaccg ttcaatggtt tcttatcaaa agaatcattt ttagaaacaa catttacagc
    12541 aagcaaagca aatttattca gtgttgatac attaggctac ctattcccaa tcattggtat
    12601 agtgggcagt gtcttcacat ttgtatattc aattaaattt ataatgcata tatttttcgg
    12661 acaatataaa cctgaacaat tacctaaaaa agcacacgaa gtatcaatat taatgctttt
    12721 atcaccagct attttagcta cattagtaat tgtatttggt ttattccctg gcattttaac
    12781 taattcgatt attgaaccag ctacatcttc gattaatcat acggtaattg atgatgttga
    12841 attccatatg ttccatggat taacgcctgc ctttttatca acattagtga tttatatttt
    12901 aggtatctta ctaattgtga catttagtta ttgggtgaaa ttgttgcaac gacaacctgg
    12961 taaactaaca ttcaattatt ggtacaacag aagtgcaaat gttattccaa actactctga
    13021 aaagatgacg aatagctatg ttaccgatta ttcacgtaat aatttagtta tcatttttgg
    13081 tgcattaatt ttattaacat ttgtcactgt attctcagta cctttcaaca ttaattttaa
    13141 agacgttagt ccgattagaa ttttcgaagt ttgtatcgta attttattat taagtgctgc
    13201 ctttttaatt cttttcgcta aatcaagatt atttagcatc attatgttaa gtgcagttgg
    13261 atatgctgtg tctgtattgt ttatattctt taaagcaccc gacttagcat tgacacagtt
    13321 tgtcgttgaa tctatttcaa cagcattgtt cttattatgc ttctatcact taccaaattt
    13381 aaatcgttat aatgaaaagc gctcattcca gcttactaat gctttaattg ctggtggtgt
    13441 tggtttgtcc gtcattatta tcggtttaat tgcatacgga aatagacatt ttgaatcgat
    13501 ttctaagttt tatcaagagc acgtttatga tttagctcac ggtaaaaaca tggtaaacgt
    13561 tatactagtt gatttccgtg gtatggatac tttattcgag tcatctgtgc ttggtatcgc
    13621 aggattagct gtatatacaa tgattaaatt acgtaaaaag cgtcaaacgc aaggtaacga
    13681 ggtgaaaaat catgaataga caacaaaatg atctaatttt gcaatttgct gcagttatta
    13741 tctttttcat ggtaatggtc tttggttttt cacttttctt agcaggacat tatacacctg
    13801 gtggcggttt cgtcggcgga ttattattcg caagttcact tgttatcatt acgattgcat
    13861 ttgatataga aacaatgcgt aaaattttcc cattagattt taaaatcttg ataggtatcg
    13921 gattagtttt ttgtattgcg acgcctattg caagttggtt tttaggcaaa aatttcttta
    13981 cgcatgtcac atttgatata ccgttattta tattagaacc tgttcatatg acaacagcag
    14041 tattctttga tttcggtgtt ttatgtgcag tagttggtac tgtcatgaca attattattt
    14101 cgattggaga gaacgaatag tggaaattat tatgattttt gttagtggta ttctcacagc
    14161 aattagtgtc tatctcgttt tgtctaaaag tctgatacga attgttatgg gaactacgct
    14221 attaacacat gcagcaaatt tatttttaat aactatgggc ggacttaaac atggtactgt
    14281 tccaatttat gaagcgaacg taaaaagcta tgttgatcct atcccgcaag cacttatttt
    14341 aacagcaatc gttatcgcct ttgcgacaac agcctttttc ttagtattag catttagaac
    14401 atataaagaa ttaggcacag ataacgttga gagtatgaaa ggagttccag aagatgattg
    14461 aaagtaatat gcttgtgtta acgctcgtca ttccagtaat tacagccata ttacttgtct
    14521 ttattggaaa acgtcctatt ataaagcgtt atgtcgcact aggcggtaca ttattaacct
    14581 tggtcgcggc aattatcaat ttagcaaatg ttgtaaaaca tggaccatta cgagttgaac
    14641 taggttcatg gaaagcgcct tacagtattg tctttgtttt agacatattc agtgccctac
    14701 taattattac tagtattatt attacagcta ttgtgatttt atattcatac caaacaattg
    14761 gtattgaacg tgaaagatat tattattatt tttcagtact atttatgtta ataggtatca
    14821 ttggtgcatt cacaactggt gacattttca acctattcgt atttttcgaa gtatttttaa
    14881 tgtcatctta tttcttatta gtaattggtt ctacaaaaat tcaactacaa gaaacaataa
    14941 aatatgttct tgttaacgtt gtatcgtcat ccttcttcgt aatgggtgta gcgattttgt
    15001 attcagtagt aggaacattg aatctagcag atataagcaa taaattagct aacctttcag
    15061 cgcatgacag tggcttagtc aatatcgttt tcatactatt tatctttgtt tttgctacaa
    15121 aagctggcgt attcccaatg tttgtttggt tacctagtgc atactatgca ccaccaattc
    15181 caatcattgc attctttggt gctttg
//
LOCUS       DAMZPP010000066        15159 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMZPP010000066 DAMZPP010000000
VERSION     DAMZPP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15159
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.066"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..219)
                     /locus_tag="POH73_001528"
     CDS             complement(<1..219)
                     /locus_tag="POH73_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HDI6887479.1"
                     /translation="MNFKLNNTISNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLIT"
     gene            complement(350..1558)
                     /locus_tag="POH73_001529"
     CDS             complement(350..1558)
                     /locus_tag="POH73_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6887480.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(2012..2371)
                     /locus_tag="POH73_001530"
     CDS             complement(2012..2371)
                     /locus_tag="POH73_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HDI6887481.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(2384..2620)
                     /locus_tag="POH73_001531"
     CDS             complement(2384..2620)
                     /locus_tag="POH73_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HDI6887482.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            2937..4001
                     /locus_tag="POH73_001532"
     CDS             2937..4001
                     /locus_tag="POH73_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6887483.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(4061..4384)
                     /locus_tag="POH73_001533"
     CDS             complement(4061..4384)
                     /locus_tag="POH73_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HDI6887484.1"
                     /translation="MEHVSVIVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            4485..4727
                     /locus_tag="POH73_001534"
     CDS             4485..4727
                     /locus_tag="POH73_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDI6887485.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(4989..6164)
                     /gene="dltD"
                     /locus_tag="POH73_001535"
     CDS             complement(4989..6164)
                     /gene="dltD"
                     /locus_tag="POH73_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDI6887486.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKDYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(6161..6397)
                     /gene="dltC"
                     /locus_tag="POH73_001536"
     CDS             complement(6161..6397)
                     /gene="dltC"
                     /locus_tag="POH73_001536"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HDI6887487.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(6415..7629)
                     /gene="dltB"
                     /locus_tag="POH73_001537"
     CDS             complement(6415..7629)
                     /gene="dltB"
                     /locus_tag="POH73_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HDI6887488.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(7626..9083)
                     /gene="dltA"
                     /locus_tag="POH73_001538"
     CDS             complement(7626..9083)
                     /gene="dltA"
                     /locus_tag="POH73_001538"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDI6887489.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLAGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVSR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(9099..9251)
                     /locus_tag="POH73_001539"
     CDS             complement(9099..9251)
                     /locus_tag="POH73_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDI6887490.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            complement(9299..9409)
                     /locus_tag="POH73_001540"
     CDS             complement(9299..9409)
                     /locus_tag="POH73_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887491.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            complement(9662..10621)
                     /locus_tag="POH73_001541"
     CDS             complement(9662..10621)
                     /locus_tag="POH73_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HDI6887492.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLATARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTVGIYGMGDIGKA
                     FARRLQGFNTKILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHNPVN"
     gene            complement(10654..11433)
                     /locus_tag="POH73_001542"
     CDS             complement(10654..11433)
                     /locus_tag="POH73_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDI6887493.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGFLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            complement(11433..11867)
                     /locus_tag="POH73_001543"
     CDS             complement(11433..11867)
                     /locus_tag="POH73_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HDI6887494.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVDIRKQFTYDYTALD
                     IKVIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            11965..12231
                     /locus_tag="POH73_001544"
     CDS             11965..12231
                     /locus_tag="POH73_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HDI6887495.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            complement(12310..12693)
                     /locus_tag="POH73_001545"
     CDS             complement(12310..12693)
                     /locus_tag="POH73_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDI6887496.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(12822..13739)
                     /gene="lipA"
                     /locus_tag="POH73_001546"
     CDS             complement(12822..13739)
                     /gene="lipA"
                     /locus_tag="POH73_001546"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDI6887497.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            complement(13823..15142)
                     /locus_tag="POH73_001547"
     CDS             complement(13823..15142)
                     /locus_tag="POH73_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDI6887498.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEQRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALDWIVTDPLDSIKEEIEH
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVKTSFEEEGKTLMSNPVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLSGVKLKEILAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
BASE COUNT         4807 a         2734 c         2187 g         5431 t
ORIGIN      
        1 tgtaattaaa cgatatgttt gatcttgtac atcaaatgca gttgtataaa tcttcccaac
       61 tttactacca ataatatgtt gatgttttag tctttcaagt gattctgtaa tatcgatatg
      121 attaaaacta atgcgctcta actgatttaa atgttctggt ataccaataa tcaatgtatt
      181 tatttcgttg cttattgtgt tatttaattt aaaattcata atgtacctcc ttagatttga
      241 tatgttactc acttcaaatt gtacaacaat aaagcccttc agtgacactg aaggacttta
      301 taaatgaata atttaatttt atgtgtttag ctgtaactta atttaaagcc tagaatttac
      361 cttttttgaa agctaaaccg ataccaccga ttttgaatac cgcacgtgta tcaatcactt
      421 ttttcatgaa tgctgctttt ttaccagcga taggtttacc aaataccata cctacaccgt
      481 catgtgaacc taaagaacaa acagttccac gatcaacgta ttcgaattct tcagttgatt
      541 caccgtttaa gatgcgttta atgtttttag caacactttc accttgttgc attgcaattt
      601 gtgctgtagt tggtaatgga cgctcttctc cagctgggat aaacgctgaa cagtcaccaa
      661 taacaaaaat gttgtcgtaa ccattgattg ttaaatcttg ctttgtaacg atacgtccac
      721 gtttaacgcc ttcaaatgat tcttccatta atttactacc acgtacacca gcagcccata
      781 ctgaagtacc tgcatttaat tgttgttttt caccatctac ttcaactacg aaaccttttt
      841 cgttacaagc aacgattggt gtagcaattt taaattcaac accgcggtct tctaagtagc
      901 taactgcgtg gttaactaat tcttctgaga acattggtaa cattttaggt gctgcttcaa
      961 cacaagtgat tttaacttta ttttgatcca caccatattt gctacataat tcaggaattc
     1021 tgtctgttaa ttcacctaag aattcaacac cagtgaatcc agcaccacca actaagatag
     1081 ataaatcgtt atcatctttt tcttttgatg ctgcatagtt agcaaatttg tcttcgatat
     1141 gacgtgataa ttcacgtgct gtgataacat tttcaatttg gaaagcatga tctttcatac
     1201 cttcgatgcc gaatgtttca ctaacgaaac ctaatgctac tactaaaata tcaaagtcat
     1261 aaataccttg atttgtttct acctttttag catcacggtc aatttttgtt acttctgctt
     1321 gaacaaagtt cactttgtct ttcttcaaga cactttccac aggatataat acatcttcat
     1381 agtttagtgt acctgctgat gcttcatgta accatgttgc ttcatagtga tattcatttt
     1441 tattaataag cgtaatttct gcttcttctg ttgatatcgc tttttgcaat ttagttacag
     1501 tttgtaaacc tgcataacca gcaccaagta caagtacttt tttacgatct tgagccattt
     1561 aatttcacct aagctttcat atttttttaa ccaaatgctg ataattacta gtaacaactg
     1621 gtcattacta cttttaccac aatgtatact agtttatcat cattttgtta tttaagtctt
     1681 ttaaattatg taaaaacctg tgatgttcac aaaaaagaca tttttaatcc aatactaatt
     1741 ctatagcttt tatgttatat tttcaagcaa gattcagtca gattgtgaaa aaattaatta
     1801 attttttcgt aacctgattc cgattaaaaa tgtatccgtt atcacgatca tttaaaatac
     1861 tattaatagt cagattctta atttttaatg agatttttaa taaaacaaaa tatctttacg
     1921 caaaaatagg acataatcaa atatgtccct aatcgtaatt gatatcaatt tgtatcccca
     1981 aaattgggtt aatcattaaa aagttatttt attagcagtt ttcaggatta cctgcaactt
     2041 ttgcagttct aaatgaacta ccacagccac atgatgcaat tgcattagga ttgtcgattt
     2101 ggaaaccgcc acccattaat gattgcttaa aatcaatagt cgtaccattt aatacgggtg
     2161 catctttttt gtctactaaa acttttaatc caaagtattc taagacttca tcattttcac
     2221 caggcgcttc ttctgcaccc attccgtatg ttaaaccagt gcacccgcca ccattcactt
     2281 taatttttaa atagccatct ggcatttcat ttgctttaag catatctttt acttcgtagg
     2341 cagctgcttc tgttaatata actgttggca tgataactcc tccttaaaaa atccaagttt
     2401 cttttatatg tgcatatata ttttgtaata attcttccgg cgaatcacct tcaacaatat
     2461 caccatttac taaagcatac aacccggctg aacatatacc acaatgtgtc aggcaaccat
     2521 actctaacac atcgacatct gggtcatttt ccagttgatt aaaaacataa tctccacctt
     2581 ttgccatgtt agagagacaa aattctacga tcggattcat acttcacctt cttatttcat
     2641 ttgttacaat attatagcat tttaaaactg gtattttaac atgatgtgct caattagcaa
     2701 caactgatgt ttcttatccc agttatgtaa tagtgcctta gttaggacaa atatttattt
     2761 gtagtcattt tcaaaaaaat atataataag catataaata catatttact tttttagggg
     2821 ttattgatga atcaaagaca cttgcatttt ataactgttc attaacaaat atatctatgc
     2881 actttgatct tttcaaaatt aaagtaaata ttaacatata aaaggggtac ttcattatga
     2941 aaaacttagt tttgttaggc ggcggatatg gtaatatgcg tatcatgtca cgcattttaa
     3001 ctacatcttt accacaagat tatacagtca cattagttga tcgtatgcca tttcatggat
     3061 tgaaaccaga attttatgct ttagctgcgg gcacgaaatc agataaagat gttcgcatga
     3121 aattccctaa tcatccacaa gtgaatacgg tttatggtga aattaacgac atagatttag
     3181 atgctcaaat tgtctcagtc ggtaattcta aaattgatta tgatgagcta attattggtt
     3241 taggatgtga agataaatat cataacgttc caggagccga agaatataca catagtattc
     3301 aaacactctc aaaggctcgg gatactttcc atagtattag tgaactacca gaaggtgcta
     3361 aagttggtat cgttggtgct ggattaagcg gcatagaact tgccagcgaa ttaagagaaa
     3421 gtagatcaga cttggaaata tatctttatg accgtgggcc acgaatttta agaaattttc
     3481 cagaaaaatt aagtaagtat gttgcgaaat ggttcgccaa aaataatgtt accgttgttc
     3541 caaattcaaa tattaataaa gttgaacctg gtaaaatata taactgtgat gaacctaaag
     3601 atattgattt agttgtatgg acagcaggaa ttcaacctgt tgaagttgtt cgtaacttgc
     3661 cgattgatat aaatagtaat ggacgtgtga tagttaacca gtatcatcaa gtaccaacat
     3721 atcgtaacgt ctatgtagtt ggtgattgtg ctgatttacc acatgcgcca agtgctcagt
     3781 tagcagaagt tcaaggtgat caaattgccg atgtgcttaa aaagcaatgg ctaaatgaac
     3841 cattacctga caaaatgccg gaactaaagg tacaaggtat cgttgggtca ttaggagata
     3901 aacaaggttt tgcatatatc atggatcgca ctgtaacagg acgactcgct tctattttga
     3961 aatccggcgt actttggtta tataaatatc ataatggtta aaaattaaaa agcaatgtca
     4021 gttcacacta ttactgacat tgctttttat tatgtgtata ttattcattc acaagctttt
     4081 ggtctataaa tcgagtgatt tgctttgttt gtatataacc atctgctaca tattcatcgt
     4141 tcattgtaat taatggataa aatagttcat cttgttctat tctttcaata aattgtaaat
     4201 catgatctgt taagttgtca ttatcttttg taatatctat atacgtatat ttaaatgata
     4261 tatttgggta ttttcttttt aatagcggct gtagccagtc ataaatatct ttcgatgttg
     4321 gcgcattaac gcaacttgca catataacat ctgccccata tactatcaca ctcacgtgct
     4381 ccatatattt atcccccatt ggtttgatag atttttatta cactatctat tataatatac
     4441 taataaagat tatcaaattc atctctcgaa aggagacttg cctgatgcct actgaagata
     4501 caacgatgtt tgatcaagta gcagaagtta ttgaacgtct tcgcccattt ttattacgtg
     4561 atggtggcga ctgctcattg attgacgtgg aagacggtat tgttaaatta caattacatg
     4621 gtgcatgtgg tacatgccca agttctacaa tcactcttaa agctggtatt gagcgtgcat
     4681 tacacgaaga agtgcctggt gtaatagaag tagaacaagt attctaatat aaagatatta
     4741 agttaacaga acatattatg aataacaaca aagacaggaa tactgaatta tttataatca
     4801 gttattacct gtctttttcg ctgtcaaatt ggactgatgg gaaagttatt ttatactatt
     4861 tatctgatta attattttat aatagccaca tatccaagcg catattacta ttctattcta
     4921 tgaaatttaa aatataaaaa tagcacatac cctcaatcaa aatcgttgag ttatgtgcta
     4981 tttgtatttt aatttttagg tttatctact tcaggttgtg gttcaccttt catatgtttc
     5041 gcaatttgct catccatata aacccaacct ttccaaccga tgtgtacggc atcactgatt
     5101 acatattttt cataatcttt atcagtcata tcgtaaattt taccaccatt atctacaaca
     5161 gtagaatgga tttttttata aactgcttga cgacgttctt tatcgatacc aatgtggtca
     5221 taccatacac cgtttgatgg aatacttaca tattgaacat ctgcaccagc agcacgcatt
     5281 gtttttacaa gtaattctaa atcttggaat tctggagaat taacattgaa ttcatagtca
     5341 cgtctaactt tacgcttact ttcttgaatt aatttccagt attgatctct aataccaaat
     5401 ttattcgatg tagtatcagc tttaccaatt tcaactgctt tttgtttcat ctcatcccaa
     5461 gaagcgtctg gttttgtagc aggtttaaca tgttctaatg gagatttatc cattgcaaac
     5521 aatgatttaa tcgcttctat tttaatcaat tgattctctt taaaaccaga aatataacta
     5581 tctttagttt ctttagggtt tttagcataa gatttcaagt attctttatt gtgtacatgt
     5641 ggaaactgta ataaacgttg tgcataacga cgttttaatt cagtagacat gtttttctgc
     5701 tggaacattt gattaatttg agtttgagac atacgagcat caaagttttg attcgttaaa
     5761 ccatgatttg taaaccattg tggtgaaata ataaatgtta atttcttacc ttttaattta
     5821 tcatactgtg atgcaagttc aactgcgtta attaagtctg tagaaccacc agcacctaat
     5881 aagaatgctt ttttgttggc gttatgctta tttaatgcaa ttgcaggatt aaatgggtca
     5941 tctttaccta attcactaga gccgtatata ggataatact tgtttgattc gtataactta
     6001 tcttgaatga gtgtgccttt tagtacttga tctgtcaatg aagttctatt atcttctaca
     6061 gtcttttcat ttactaatcc tgtaaaccaa ctagcaggta ataatagaaa gacaatgaat
     6121 accgctccac taattaaaat gggtaaaaaa ggttttaatt tcatcgtaac tcttctaatg
     6181 cttcaacgat tttatttggt gttgcccact catctctatc aaagtccata atagatactt
     6241 cgatatcaag tttattttga atctctaata ataatccaac tgtttggaaa gaatcaataa
     6301 taccttcttc aaaaatttct acgtctggat tttcttttac aatatcattt tctgctactt
     6361 ctgctaataa atttaatact tgttctctaa attccataat taaattctcc tttattatat
     6421 aagtttacct gagaagatta aaaagccaaa tgttacaaag tggaatgtaa tcacaatgct
     6481 aagtgctgtt gtgaaaccat tttgccaacg tggcggatgt ttcttgcgcc aacgttcata
     6541 atagccataa cctataaaca atgctgcatg gtataaacca taaacaatgt aatacacttc
     6601 gataccatgc caaattccca ttatgaagaa gttgattaag aatgccacgt tagacattgc
     6661 aaattgactc ttcaataatt ttttacgaga catgtagaat aaagatctca tgtaaataca
     6721 atctctgaac cagaatgata atgtcatatg ccatctattc cagaaatctt taatattttt
     6781 cgctttgaaa ggtttatcga agtttggtgg tgttttaata ccgaataaat aactaaatgc
     6841 tatcgcaaat aaactataac ctgcaaagtc aaagaataaa tataggctgt atgcgtacat
     6901 atataaccac aaattgacaa agccatgtaa gtctaattgt aacggcatga ttgcatatgt
     6961 gttaataaag taagcaacaa tatatttata caagaaacca agcataatca tgtgaattgc
     7021 ttttaatact aattcacgat attcattgcc tgttggtact tttttatcgt ctttaacgaa
     7081 acgtttgtaa cgatcgattg gtccagatga aatcgttggg aagaatgaaa taaattgaat
     7141 taatttccat actttaattt ctttgataga accatcacga atttccataa ttaactgcac
     7201 acttttgaat gtaacataag aaattcctaa gaaaccaaca aattcaatta atttactttc
     7261 atggaagtga atctgatgtc cacctaacca tgtactttgt aacactttca caagtgctaa
     7321 tggcaatatt gataaaacca ttacagttac aaattttgaa aatgaattat tttttggttt
     7381 tgaatgataa taaaacatta tcaataaaac ttgccatacg acgtaaataa taaaactaat
     7441 taattgaaca cttaaatact tttggtcaaa cagattatgt ttatcagaag aaaagattaa
     7501 cacaatcata aatgctgtaa ctacgccatt ataaatgtaa cttcgcttac ctaaaaatcc
     7561 aagtataatg actggtaata atgcaattaa agcaattaag aagaatgtaa aatcaccata
     7621 tggaatcatc cgttaattac ctctgcaatt ttctttctgt caattttacc atttgaagtc
     7681 aatggcaatt gttccatcca ttcaaacttt ctaggaatca tatactctgg taagcgtgat
     7741 ttcaagtcat ttttaatatt tttagtcatt tctgcattgt ccgtaacttc agtcgttggc
     7801 acaattgcac caattaaatg aataacttta tcatttttat atacaggtac aacaatcgct
     7861 tcttttacga actcagactg gcgtaattgt gtttcaattt cttctaattc cattctgtag
     7921 ccattcaatt tgatttggaa atcaatacga ccttgaatga accattgacc attttcaaac
     7981 ttcgctttat caccagtgtg ataagtacga ataccgtcat cgaaattaaa tacttcagca
     8041 gttttttggt cattttttaa gtatcctaaa cttacacttt gaccttcgat aacaagttca
     8101 ccttcatctg tcgtagataa tcttgcgcct ggtctttcaa cgccaacagg taatgtcgga
     8161 tattgatcta agatttcttg tgtaatttga atacttgtaa ctgctaccgt agcttcagtt
     8221 ggaccatatg tgttgtaaat cgtcgcactt gggaaacggc ttactaacgc ttttgctgct
     8281 ctgtgaggta gaatttcacc acagaagaag aattcgttaa gactaccata ctgttcttca
     8341 ttaagcgttg gtaataataa acacatttcc ataaatgatg gtgttgatac ccaaatgtta
     8401 atcggtgttg ctgttagcat ttcatttaat agtttaggtt tattaatcat gtttttatct
     8461 acaagattta atgtaccgcc tgacgctaaa catggataaa tagccattac agataaatca
     8521 aatgaaaatg gcgcttggtt aagccattgt tgttcatttc ctgatttatt aagttctaac
     8581 atccactcag taaattgaac taaacttgca tattcaattt gaacaccttt aggttcccca
     8641 gtagaaccag atgtaaagat tgtgtatact gtgtcgttat ctttaatctg actatcaaaa
     8701 attactgggt cttgagatgt tttaatatct tctattgtaa atacttcacc tgctaaactt
     8761 tcaaacgatt catcagtcgt attaaataca aactctggtt gaaccttgtt aataatcatt
     8821 ttaatacggt cttcaggaat tgaagtgtct acaggtacat atccacatcc tgctttaatg
     8881 gcaccaatca tcccaacaat catatatggt gacatgtgac cgaataaaat catcggtttc
     8941 ttactacctt gtaatcgatg tgctaattta ctagactcat ccattaactg ttgataagtt
     9001 aattcatcag ttgtgtgtct aacagcaatg ctttgtggat ttgcatccgc aaacgcttgc
     9061 agcttgttaa taatatctgt catattaagt ctccctcatt agaactcatt ataaatgaag
     9121 ttattgtgtg tgtcgccact gccataaatt aaatataaag taataaatat tgccaaatac
     9181 aatagtgtta ataaatatgg tttgaatgct tcaacatatt tattaggtgg ctgtttactt
     9241 ttagatttca tattgcacct cttaaagttc ttagtaaaaa cgcctttata aagaccgttc
     9301 aatataaaat acgttttaaa atttgttttt tacaattcat tatatcgata ttcataatga
     9361 aattcaattt taattttata gattcaacat agtaattggt tgtcatcact caattattgt
     9421 aaatgataca ctatgttgag aacatcattc attttaaagg ttatttaaac aataaacaat
     9481 tacagtctat ataacaattt tgttatatac gtcaaaatca aataaactca tcacattaat
     9541 atgacgagtt tataacgtta ttgaattatc atcagcgcaa atatatacat tcgcatgtca
     9601 agcataacat atttaacaat tgctttgctt gtttaccaat gattaaaaac catacttatt
     9661 ttcaatttac tggattatgt ggtacctgat ttgtcataac cgcttctata ttattaatac
     9721 ataattgaat catattgtcc cgtgttgtta ctgatgcgct accaatgtgc ggtgtaatca
     9781 gaacattatc acgtcccatt aatggatgtg tatgatcaat tggttcattt gctaatacat
     9841 ctaaaccaca tgctaatatt tctttattgt ctagtgcatc gattaatgct gtttcatcta
     9901 caatttgccc tctaccgata ttaataaaaa ttgcatcatt tttcatttgt tcaaatgctt
     9961 cagcattaaa tttatgatgt gtttcttttg taagtggcgc tgtacagatg ataaaatcac
    10021 tttctgctaa caacgtttca aaagaaacat atgttgcatt aaagtccgcc tctgcatctt
    10081 tatgtcttga tcgattatga taaagaattt tagtattaaa cccttgcaac cttcttgcaa
    10141 aagctttacc aatatctccc ataccatata ttccaacagt tgaattgaag acatctttac
    10201 ctgacaataa ataaggaccc cagctttgcc atgcatctgc ttctacatat ttttcagctt
    10261 ctacaatacg acgtgctgta gcaagcatta atgtaaatcc taattctgca gttgtttcag
    10321 taagaacatt tggtgtattc gtgacaacca cgttgttcgc tgttgcactt tcaacatcga
    10381 tgttgtcata tcctacagcc atattcgcaa ttacttttaa atttggtgat tgtgctaaaa
    10441 tttctgcatc gatctgttca cttaatgtga taaaacaagc atctgcgtca cgaagtgctg
    10501 tcacaaattg atctttaggc ataggcacta atgatttttg ccacataaca acgtcaccaa
    10561 gtttactcaa ttgttgataa aatttatctg gaattttcct cgaaacaact atttttacca
    10621 aatcaattca cctactttat aaaatgacta tatctatttt tctaattcag ctattgcttc
    10681 atttaaatct ttaaaagaat acgttggtgg cacattttta ttttgcacat cttctaacgt
    10741 agatacacct gtttgtacat gaatcgtatc catacctacg ttaataccag acataatatc
    10801 ggtatcgtac aaatcgccta ccatagcaac ttcggattta tctagtccta aaatttctaa
    10861 tgctttaacc ataataatcg gttctggttt accaataaat tgtggcgata cacctgtcga
    10921 tacacttaca acacttgtaa tagcaccatt ccctggtaaa aatcctcgct cttttggaat
    10981 tgacacatct ggatttgtag aaataaatgt tgccccattt cttacaccta acgttgcaat
    11041 cgcaagcttt tcatatgtaa cttgttcgtc aagtccaata actacataat caacatgctc
    11101 gtcattttta atgacaagtc ccgcttcggt taacgcagta tttaaaccac taccacctaa
    11161 catatatact gatgctcctg gtgattgttc tgaaatataa tctgcagtgg ctaacgctga
    11221 cgttacaacc tcttctggtt tagcatcaat gtgcatttca cgtaattttt cagttacttg
    11281 ctcaggtgtt ttcgttgaat tattagttac gtataaatga ggaatacctt ttacatttaa
    11341 ataatcgatg aattgttttg ctccatcaat ctcatctgtt cccatataca ttgtgccatc
    11401 taaatcgatt aaatacgctt tatactgttt cattattgat tttctccttt accaaaagct
    11461 gttactggta cattttcttg atgtaaaaat gtcgttactt ctgtaataaa ttgtttgtaa
    11521 taaggtaatg cgtcatcaaa cattggcatg ataaccttaa tatctaaggc tgtgtaatca
    11581 tatgtaaatt gtttacgaat atcgacagtt ttattaatcg cctgctgtgt ttctttagta
    11641 ataacatttt ctagttctaa tatatcaatc acatctttat aattaccagg atcccttaaa
    11701 ataaatgcat cgataatcat attccctata tctaccgatg attctatcaa catttgagca
    11761 atgcgttcaa atgcataatg attgtgctta ctctcatgat aatcatcagt taatgcttgt
    11821 aaataggcta atttctgagt tagtttatct ttgtctacaa aatacattcc ttgtcacctc
    11881 tcgcttatat attatcataa ttttgcagta tacttcattt gtcgttatac taactttact
    11941 acttttaaaa ggagtcgcta caaaatgatt gatatgtatt tatatgatga caacgaagag
    12001 agtcaagttc agtttgttgg ttttgttggc gagcatagtc gatatgattt aatgctagtc
    12061 catacaaata gacattatgg taagacactc gtacttaata tgcaaacaaa taaattcggt
    12121 attatcggta ctgatgattt aaaagaagaa ggctatattg ctcatatttt aggtgtaaat
    12181 gctgaagaag gcgatgaaat tacagagtat ttaaatgaag tcattcatta aagataaaaa
    12241 cccctaacta gattacaggt ccatgcttga attgcatgat tcccattttc aatctagcta
    12301 agggttttat taatgttcag tatcttgaat ggttggctta atcttcacat cttgaagttt
    12361 attgtcgtca tctatcacat cagacggatg aacttcttcg attaactttt taacctcttg
    12421 tttagacaaa tgtcttaata caaagtaagg acaaccaaag ttacaatatt caaatatgta
    12481 atcttgaata tttgaaaaac gtttactcat ctctgctttt ttattagaat ctttgtaaaa
    12541 accttttaat cgtaattgat cgtaaccata gtcaccaaca atgtaatcat atttatctaa
    12601 aatatctgaa tacctagcaa taaattgttc ttcattaaaa cattcgcgat aattttctat
    12661 taattcaaag taatgttgat ctacttttat caaattcttc acctatctat gacaatgaaa
    12721 ggctcagttt atactttagt gtttcataaa aataatgatt acttctatga cacgttcgtt
    12781 ttgtacaaac taagccttta tgccttatta atggttaaat attaactatt taactgtgcc
    12841 tcaccttggc gttgcttttc tttagcagct tcatttactt gctcatccgc atgatatgaa
    12901 ctacgtacta aaggtccagc ttgacaatgt ttaaaccctt tatccattgc cacttttctt
    12961 aatttaccaa actctaaagg cgtgtaatat ttttgaacct ttaaatgttt acgtgaaggt
    13021 tgtaaatatt gaccaatcgt taaaatatct acatcattcg cacgtaaatc atccatcgtt
    13081 tcataaattt cttctatagt ttcacctaat ccaaccataa tacttgattt agttgggata
    13141 tccggttgta attcttttga acgacgtaaa aactctaatg ttctgtcgta tgtcgcacgc
    13201 gcacgaactc tcggtgttaa tcgacgaaca gtttcaatat tatggtttaa aatgtcaggt
    13261 cttgaagcca ttaatgtttc taacgcatca tagtccccgc ccatatctga tggtaaaatt
    13321 tcaatcgttg taaatggatt tctttctcta actttacgta ctgtctcagc ataaacattt
    13381 gaaccagcat ctcttaaatc atcacgcgca acagcagtga taacaacgtg tttcaagttc
    13441 attaattcaa ctgattcagc tacacgttca ggctcattta aatcaagttc attaggtaaa
    13501 cctgtcttaa ccgcacaaaa acgacaagct cttgtacata cggcacctaa aatcataaat
    13561 gtcgctgtac gacgtgcacc ccaacattca tgtatattag gacatttagc ttcttcacat
    13621 acagtattaa gatttttttc cctcatcatc ttcttaagtc ctgtatagtt ttcgttggta
    13681 tttaatttta ttttcaacca atccggttta cgtaatattt cctcgttttt tgtcgccata
    13741 acaacacata ccctcctgtt aagtgtatct ttaaacatta tatcgaaatt catgacaaat
    13801 gaaaatccaa taatcttact ttttaatatt ccaataattt ttctttaaat atatctctta
    13861 aaaactctgg cattaaatac tcttttggta attctttcaa tgttgggaaa taacgaccga
    13921 acgtatacat atctatcgta cctaatacat attctttgtc gtcttcgatt tcttctccat
    13981 tcagatagta acgccctgta gaatgaatgt accctaaatt ataaagaata tatcctccaa
    14041 atatattccc tctgaaaccc aaaccttgtg catgttcata catatattct tgtttattac
    14101 ttttagctaa aatctctttt aatttcacac cagaaagtct aacccttacc atattaatcg
    14161 gatgcggtaa catttgatga atgtcatatt cagtcacttc atcttttaca atatctttaa
    14221 cgagtaagcc agcattgatg atagcacatt gagcatgtgt atactcacaa acactttgag
    14281 ctaataagta tgcagcttca gttatgtggt tcatactacg cttaagcact actggatgtt
    14341 gaattactgg attagacatt agcgtttttc cttcttcttc aaatgaagtt ttaacttcag
    14401 gtaatgtttc taaaggaata atctttgcag ttttatgtac tactttatgt gcctcaaaag
    14461 ttaaattaac ctctccaaga taattaccat actttccagc tgccgccatc agtacaccat
    14521 tattaatttc accatgttca aaataatgat gcgtatgact accaaaaatg acatcaattt
    14581 caggcaattc ttggcataat gtttcatcga agaaaatgcc acaatgactt agcacgatta
    14641 atacatcaaa tttacctcgt tgatgttcaa tttcttcttt tatagaatca agtggatcgg
    14701 taacaatcca atctagtgca cgataaaatg gggtaaaagg tgctgtcgct gcaacgaata
    14761 gtattttcac accgtctata tccttaatat aagaagaaac gatattattt ggtaaatgac
    14821 ctgattcatc tataacattg ctacatgtca ctataaattt tgcttcgtcg taaaggtgat
    14881 ttaaagcttc gtgtgaaatg gtcatccctt cattattacc gattgttgca acatcacatt
    14941 ttgcttcatt tagtaatgct acattctttt tacctaaagt tgcttctgtt ataggtgcgg
    15001 ataaatctac atgatcacct agatcaacat ataaagaagg atgattaagt cgtggccgtt
    15061 gttctgccat atatgcttta atgcgttcgt attcatgtaa atgactatga atatcgttcg
    15121 tatgataaat tgtaagcctc aaattgacgt cctcctttt
//
LOCUS       DAMZPP010000067        15147 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMZPP010000067 DAMZPP010000000
VERSION     DAMZPP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15147
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.067"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..1679)
                     /locus_tag="POH73_001548"
     CDS             complement(<1..1679)
                     /locus_tag="POH73_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDI6887499.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEQRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKSKTHNI
                     YSNNTNHRAKDSTTDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKSSEKVESDKQ
                     KYDKYVAKTQTSQNKHLEQEKQKDSVVKQGTASKSSDENVSSTTKSTPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTEASQLHTNEIEDEAVSNDENKKASIQNEDTDDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSDVTSQRQSALQHNVEVNNQDELKNQSRLIADS
                     EEDGATNEEEYSGSQIDDAEFYELNDTEVDEDTTSNSEDNTNRDASEMHVDAPKTQEH
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDGHVVEDS"
     gene            complement(1700..2296)
                     /locus_tag="POH73_001549"
     CDS             complement(1700..2296)
                     /locus_tag="POH73_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDI6887500.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEETGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            complement(2325..3182)
                     /locus_tag="POH73_001550"
     CDS             complement(2325..3182)
                     /locus_tag="POH73_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HDI6887501.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            complement(3282..3593)
                     /locus_tag="POH73_001551"
     CDS             complement(3282..3593)
                     /locus_tag="POH73_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI6887502.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            complement(3658..4734)
                     /locus_tag="POH73_001552"
     CDS             complement(3658..4734)
                     /locus_tag="POH73_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDI6887503.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPAFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            4821..5132
                     /locus_tag="POH73_001553"
     CDS             4821..5132
                     /locus_tag="POH73_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDI6887504.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            complement(5256..6098)
                     /locus_tag="POH73_001554"
     CDS             complement(5256..6098)
                     /locus_tag="POH73_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6887505.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHITADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVPGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDDGESIDAYILRETL
                     VDNN"
     gene            complement(6544..7359)
                     /locus_tag="POH73_001555"
     CDS             complement(6544..7359)
                     /locus_tag="POH73_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6887506.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSS
                     RYYTNWNTIEKQVNHRGWEDLASKTTDDILSVNKIFYTAPAEKQTRLSNDLYSISASI
                     DLGQKLHIVNKYGHDHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMI
                     FIGMPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDY
                     EAITTIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
     gene            complement(7352..8794)
                     /locus_tag="POH73_001556"
     CDS             complement(7352..8794)
                     /locus_tag="POH73_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDI6887507.1"
                     /translation="MKNKEKYLTNFSEAKRKEATQKYNIIKPFILGKQSLSSISKSKG
                     IALSTFYRWNKLYKEQGLTGLIHNTRVDKGEHKLKQNIIDEIKRLALKNKRNSIATIH
                     RKIANYCIENNFYKPSYKQVYSIIKAMPKSVIDFSHQGEKYYQNKYDLIQIRESSRPN
                     EIWQADHTLLDIYILDQKGNINRPWLTIIMDDYSRAIAGYFISFDAPNAQNTALTLHQ
                     AIWNKNNTNWPVCGIPEKFYTDHGSDFTSHHMEQVAIDLKINLMFSKVGVPRGRGKIE
                     RFFQTVNQTFLEQLPGYINNNDTSSDLIDFQNFEEKLRYFLIEDYNQKEHSAIQSTPI
                     NRWNSNHFFPNMPSSLEQLDLLLLEIPKSRKIHSDGIHFQGFRYSNTNLTAYVGEYVL
                     IRYNPNDMAEIRVFYRDEFLCTAISPDLADYSIDIKEIQHARSQRRKHLKQNIASPST
                     TDLIKEEKSYGYSPQETTKNVKKLKRYRYD"
     gene            complement(8766..9359)
                     /locus_tag="POH73_001557"
     CDS             complement(8766..9359)
                     /locus_tag="POH73_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6887508.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNAYGCEKIFSDHISGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEEKEDEKQGEVSN"
     gene            complement(9623..10003)
                     /gene="blaI"
                     /locus_tag="POH73_001558"
     CDS             complement(9623..10003)
                     /gene="blaI"
                     /locus_tag="POH73_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDI6887509.1"
                     /translation="MANKQVEISMAEWDVMNIIWGKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(9993..11749)
                     /gene="blaR1"
                     /locus_tag="POH73_001559"
                     /pseudo
     CDS             complement(9993..11749)
                     /gene="blaR1"
                     /locus_tag="POH73_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
     gene            11856..12701
                     /locus_tag="POH73_001560"
     CDS             11856..12701
                     /locus_tag="POH73_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaZ family penicillin-hydrolyzing class A
                     beta-lactamase PC1"
                     /protein_id="HDI6887510.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKALIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKENKKFLLDLMLNNKSGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(13175..13819)
                     /gene="trmB"
                     /locus_tag="POH73_001561"
     CDS             complement(13175..13819)
                     /gene="trmB"
                     /locus_tag="POH73_001561"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDI6887511.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGEMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKNGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            complement(13834..14625)
                     /locus_tag="POH73_001562"
     CDS             complement(13834..14625)
                     /locus_tag="POH73_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDI6887512.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
BASE COUNT         4799 a         2611 c         2015 g         5722 t
ORIGIN      
        1 cttgaatctt ccacaacatg accatcattc acatcattcg ttttcaatga gttgacactt
       61 aagtttgttt ggtcatcttt tttatgacgt ggcgctaatt caatttcatt atcaaccgtt
      121 ttatcgatat tattaacttg agattcagtt actgcgtgct cttgcgtttt aggagcgtct
      181 acatgcattt cagacgcgtc tctattggta ttatcttcgc tatttgaagt agtatcctca
      241 tctacttctg tatcatttaa ttcataaaat tctgcatcat cgatttgact tcctgaatat
      301 tcttcttcat tcgttgctcc atcttcttct gaatcagcaa ttaatctgga ttgatttttt
      361 agttcatctt gattatttac ttcaacgtta tgttgcagtg ctgattgacg ttgagacgtt
      421 acatccgaaa ccgtaacttc atcatctgac aattgttttg ttgtcgatac ttgattcaaa
      481 ctaacttcct cataattgta tggactttca tctacatgag tgtcatcagt gtcttcattt
      541 tgtattgacg cttttttatt ttcatcattt gatacagctt catcctctat ttcatttgta
      601 tgaagctgac tagcctctgt actattatct gttaagtcac tatcatcatt taatgaacta
      661 tcaccaacat agcgttccgc ttgtttagca tacatttcat caattgcacg ttgtattgca
      721 tcttgctctt cgtttttatg ttcttcacgc ttttgttgca acgctttttt aaatcgacgc
      781 ttttgaagca ctttacgttc tcgttcacgt ctaatttctt caacaatttg tgaagcataa
      841 atgttttcaa ttttgatagt attatcaact tttgaataat taggtgttga ttttgttgtt
      901 gatgatacat tttcatcaga tgatttagat gcagttcctt gcttgacaac actatctttt
      961 tgtttctctt gttctaaatg tttattttga gacgtttgcg tcttagctac atatttatca
     1021 tatttttgtt tatctgactc aactttttct gaagatttac ttacagggat acgaccattt
     1081 tctaactttt taggtttcat tgtgccaaaa atagctgacg gtacctctga agtcttgaaa
     1141 ctttctttgt gataatctgt agttgaatct ttagcacgat gatttgtatt attagaatat
     1201 atattgtgtg tttttgactt gtgcgtttcc ttctcaatac cattaattgc agaaacatat
     1261 gtacctggct tattcgtatg gtaatgtgaa tgatctttat attttatact ttgctgtgat
     1321 attttagaat tcccacgttg ttcactataa ttttgttctt cagttgtttg atttcttcta
     1381 cggcgatgtc gtttttgtga acgagaatcg tggctttgtt tgcgatagtc tcgatggtat
     1441 tgttcttttt catctgaaat agtatctgca gattgttcaa cattttcatt ttcataagct
     1501 acgctcatag gaaaacggaa ttttcccctc ggacgactat aaatatcatt attttgaggc
     1561 agtaatgagt catgatcgtt atctatattt tgtgattctt gacgtctttt tttctttcta
     1621 tgaatcaagt catcatttga atcattatct tcgccgaata atttatcaaa ccagctcata
     1681 ttttcactac cttccttaat tattcaaaaa atgcttgtcc aatttcatag ctgtcattta
     1741 ataccataat acctttttct tcaggtgcat taggtaaatt caattctttc attgaacaaa
     1801 tcataccgct tgaggcaaca ccacgtaatt cagcatcttt aattaccata ccgctaggca
     1861 tcactgcacc tactttagca acaacaactt tctgtccagc ttcaacgtta ggcgcgccac
     1921 atacaatttg taatgtgtca tttccaacat ttacatttag tacacttaat ttatctgcat
     1981 caggatgttt gtctttagtt tcaacgtagc caactacaaa tttcggtgat agatcagcat
     2041 ttaatttata atcaaaacca gcttctgaaa tacgcttttg gaatacattt acaagttcat
     2101 cagttaattt aatatgacct gtttcttcaa ttgttatatc ttttgaaatt tcaaaaatat
     2161 tataacctac aacattacct tcattagtaa tttcaacaac gttacctttt ttattgtagt
     2221 ttaattcacc ttcaactggt tcaatttgta aaaatgcgac atctcctaca tatttaggat
     2281 tgtaaaataa attcattatg acaaaactcc ttatccaatt atttctattt atctttatta
     2341 aatttacgac gatttgcttc taaacgctga atcacgtttg gatctctttt ttgtttatta
     2401 tttttaccta aaataaatat cggttcaaga tgaccctgtt tatatccaaa ggataatgat
     2461 gtaattggaa ctagaccttt agtgaaaaat tccattgtta aatgtgccat cacatcatat
     2521 cctgttttat tgcgtatatc tgcaataatt aacacatctt ggtgtggcac tgctacgagc
     2581 atttcacctt gacattgtgc ctcaatttca tttaaaaatg cagtatttag aatcctactt
     2641 gcatcatacc cgtcatttga gttaataaag taaaaaatat taccttttac ttcatcagtc
     2701 gtatatgaat ttgataattt tctaacatta aacagagaca tttctcttat ttgttgttca
     2761 gttaacttca aatcctctaa catgctttca tcaattagac gatatgattt tcctaaatcg
     2821 actgcataat aaactgctgt ttctgcagta tgttcatcat agataaaagg aacaccttgt
     2881 ttagtttttt tatcaaagct agtcgctcta atgacaggca taatttgact agatgatata
     2941 ctctcaaggg ttttatctgc catttgtgca atagcttcat caacgtaata aacaatttca
     3001 tctacaattt tttctttttt atcttcatat tttgcgacta tagcgttaag tttaatcgtg
     3061 atacctttgt tattatctgt tcgataaata cgcaaagttt cttcttcacg attaaattta
     3121 aaatcaacgt ctaaatggct taaacgttcc tttaatttat ctctcatttg aaaggtattc
     3181 atatgtaaca ctcctaattt cgcaccttat aatagtttac aataaatcaa cacatagtaa
     3241 cagagaaaaa cgagtattac cccgtttttc tctagtctaa attacacgta ttgagctaaa
     3301 aatgcatcta tctgttcaat tgattttcgt tcttttccaa tataacttcc aagcagttct
     3361 ccatttttat atactagaaa acttggaatc cccataatac cattttcaat acaaatatcc
     3421 ataaatttat cacggtctac tgatacgaag tcaaacatag gatatctcgc ttctaattcc
     3481 ggtaaatctg gttctatcac tctacaatct ggacaccagc ctgcagtgaa ttcaaatact
     3541 gtagcacctt gttttaaaga ttcaaattgt tgttctgatt caagttgttt catttttatg
     3601 tctccttaat taatgattat atataactat ttagaaataa tagttgttat tacccgatta
     3661 tttatattgt aatgtttcta tttgattgtt atctaaatta caaatggctt ctgaaagtaa
     3721 agatctagca gcaaaatagt ctcttatatc aaatactgag tctgtactat gaatatatcg
     3781 tgcacataca ccaataactg cagttggaat accaatatta gctttatgaa tttctccacc
     3841 atctgttcca ccaggtgaca tatagtattg atgttcaatg tcatgtgctt ctactaactt
     3901 taataaatag tctctaaatg caggctttaa aatcattgta ccgtctttta tgcgaattaa
     3961 cgtaccttta ccaagttcac cggataatgg ttgattacct ttaacgtcat tggcaggtga
     4021 acaatcgaca acaaatgcaa cgtctgggtc tatcatctct gcagatgctt tcgcacctcg
     4081 taatccaacc tcttcttgaa catttgcgcc aacatacaag tctacatcta attctatatc
     4141 ttttaataat tctagtattt caattgccaa gacacaacca taacgattat cccatgcttt
     4201 agcactatat cgatgttcag ataactgtgt gaatggcgtg tgaggtacaa ttgtatctcc
     4261 tatatctatt ccgcgctcac gcacttcatc ttcattttga gcacctatat ctaatgttaa
     4321 atctttaatt tccggtgcac cttcactacc agtacgaaaa tgtttaggta tattagaaac
     4381 aacaccgata attttatcgc catttctatt tttaattact aagcgttgtc cttgccaaat
     4441 atcatttgca acaccaccta aatttgtgaa ttgaatcatt ccatttttag tgatgtttgt
     4501 aatcatgaat ccaatttcat ccatatgtgc tgcaatcatt acacgttttg catttggatt
     4561 tttagatttt ttaacaccaa aaaaaccacc catacgattt tcaataaatt catctacgta
     4621 cggcgccatt tgctgagtca tataattttt tacttcttct tcaaaacctg gtgccccatg
     4681 aagctcagtt aaagtttgaa ttcgttgtaa tgttactttt ttatttatgt tcataaaagt
     4741 tctcactcct tataagttat tatatcatct tcaatatggt aaactaatga tatgaatttg
     4801 aaatcaaggg agcataaatg atgactaaac tgaaatatat aattccaaca ataattgcag
     4861 tagccattgt aattatttct accatttcaa tcatccaatt tattaatcgt aaacgctata
     4921 atcccgttaa agtacttaat gaagtaaaat catattttat gaatgtcaaa ggctcatata
     4981 tcgtttatga accattcgtt catcctgaaa ctgataaata ccgtttagtt tatcaaggtg
     5041 gaattacaac tattaaaaat ggtcaaaata ttcattatga tttttatgca gatgcatata
     5101 ctggtgaagt cattaacatt gtagagcgtt aatacgaatt ttatccatat ttgatttgga
     5161 tcgtaaaact taaaaatgag ccacacccta attagtaaga taggatgtgg ctcaattttt
     5221 tattcttgaa aatttgttta gtaatacatc tttttttagt tattatcaac taatgtttca
     5281 cgtaaaatat atgcatctat actttcgcca tcatcgctgt attttacagc aaagtagttt
     5341 tcatcatgat aaaacaagaa ccaatattgt tgctggataa aatatggtat catgcgttcc
     5401 ttttcacgaa ttgattgcat aggataatca tcatatgctg ttacccatag aggattttta
     5461 tgtgcagtag tcgggaatat atcacccata tgaactgctt tatctccttg actttcaatc
     5521 gtaatgattg tgtggccaaa gctatgacct ccactatgtt gcatcttgat acccggaacc
     5581 ggttcaaaat gtttttcgaa taaaatcaac ttgttactat aatcgccttt attcttatcc
     5641 cagtaagttg atttacttct tatattaggt gcaataaact catgccactc atcttgttgc
     5701 acaacatgaa tcgcattttc aaaaattgca tgtcccgctt gatcagtcaa accggcagca
     5761 tgatcaaaat gcatatgtgt cattagtaca taatcaatat cctttggcgt taaattataa
     5821 tttgccaaat cagcagttat atgactttct tcatctacac caaaattacg taattgcttt
     5881 tcagataatt taccattacc aatacccgca tctataatca aattatattg agccgtttga
     5941 atcaaaattg gatgtgtcgg taaattgatt tgatttcgtt catttgcatt gtattgcttt
     6001 gaccacaacg gcttcggaac aacaccaaac attgcaccgc catccatttt tgtattgcca
     6061 ccatttagat aatgaataga tatatccccg attttcataa catcacctat tctttctata
     6121 ttcgttatta caattagcat gtgtttatta catcatatca ctattcataa atgcgaacaa
     6181 aaatgaaacg ccccacacta atacacttcc aactacttat aaaaattaat gcaatcatat
     6241 tatatgtaaa tgcatcataa ccatcggact gtaattttaa attaataatt aaaatttcca
     6301 agtaaaatgc aagctttatt ctttcaaatc aaaggtattt agtataacat tgttcatagg
     6361 cgcattgtct cctttagttt gggtttggtc actttaaatt atagaggcaa tagcgctttt
     6421 ttgtattaaa aaacaaaaag ttccaccttt ccaccatatg ttactttact tgatatatga
     6481 gaatgatttt acctagtaaa tgagaaaagc aatgtcctat attaaagtac attgcttttt
     6541 ctattaacga atacctatga ctaaactatc tctagctgtt tctacaactt ctgtactgat
     6601 tttatctaag ccatttatat ccataattct atctatctgc gcaaataaac gatgaatcag
     6661 cctaaaattc ccttttgtaa ttttaataat cgtcgttatt gcctcataat cagtaaaatc
     6721 ttcgagtttt agatcaaatc ctaaatcttg ccacttatat tctaatatat gatgcgtctc
     6781 gtctttactt agattatcaa attcatgtgc aaaacctata cgcgaatata gttgaggata
     6841 acgagatagt tttttctcta tacctggcat accaataaat atcattgcta aattatgttc
     6901 atcataaata gctcttaatt gctctaaatg ttgcacttta aggcgatcaa tctcatctac
     6961 tataattaaa tcaatatatt taaacatatc actataatgt ttactatgat catgaccata
     7021 cttatttacg atgtgtaatt tttgacccaa atcaatactt gcactaatac tatatagatc
     7081 attacttaat cttgtttgtt tttcagccgg tgcagtataa aatatcttat tcaccgataa
     7141 tatatcatca gtcgttttac tagctagatc ttcccaccct ctatgattaa cttgcttttc
     7201 aatagtattc caatttgtgt aatacctaga agataaagtt tttccgacac caggttgacc
     7261 atagcagatc ccaatatatt gatatttgat acaagcatcg caaaattcag caaatcgttt
     7321 atactctttc gtttcaatga aattctgatt tttagtcata acgatacctc tttaattttt
     7381 tgacgttttt ggtcgtttct tgaggtgagt aaccataact tttttcttcc ttaattaaat
     7441 cagtggtgct tggactagca atgttttgtt ttaagtgttt tcttctctga ctacgtgcgt
     7501 gttgtatctc ttttatatca attgaataat cagctaaatc aggggatatt gctgtacaaa
     7561 gaaattcatc tctataaaaa acgcgtattt cagccatatc attgggattg taacgaatta
     7621 atacatactc acctacataa gcagttaggt ttgtattact gtatctaaag ccttgaaaat
     7681 gaataccatc tgaatgaatt tttctagatt taggtatttc taataataat aaatcaagtt
     7741 gttctaaact gcttggcata ttaggaaaaa aatgattact gttccaccga ttgataggag
     7801 tgctctggat agcactgtgc tctttttgat tataatcttc aattaaaaag tatcgtaatt
     7861 tttcttcgaa attttgaaaa tctattaaat cgctagaagt atcattattg tttatatatc
     7921 caggaagttg ttctaagaaa gtttggttaa ctgtctgaaa aaaccgttct attttcccgc
     7981 gaccacgagg gacaccaact tttgaaaaca tcaagttaat tttcaaatca atagcgactt
     8041 gttccatgtg gtgcgaggta aagtcactcc cgtgatctgt gtagaatttt tcgggtatac
     8101 cacatactgg ccaattggta ttattcttat tccatattgc ttggtgtagt gttaatgctg
     8161 tgttttgagc attaggagca tcaaaactta taaagtagcc tgcaattgca cgactatagt
     8221 catccataat aatggttagc catggtctat tgatattacc tttttgatct aatatataaa
     8281 tatctagcaa agtatggtcg gcttgccata tttcattggg tcttgaggat tctcttattt
     8341 gtattaaatc gtacttattt tgatagtatt tttcaccttg gtgagagaaa tcaataacag
     8401 acttaggcat tgctttaata atactataaa cttgtttata acttggttta taaaaattat
     8461 tctctataca ataattagcg attttcctat gaatcgtagc aatactattt cttttattct
     8521 tgagtgcaag gcgtttaatc tcgtctatta tgttttgttt taatttatgc tcacctttat
     8581 caactcttgt attatgaatt agaccagtaa gaccttgttc cttatataat ttattccacc
     8641 tataaaatgt acttaacgca atacctttgc ttttagaaat gcttgataaa gattgcttgc
     8701 ccaagataaa aggttttata atattgtact tttgagtagc ttctttacgt ttggcttcag
     8761 aaaaattagt tagatacttc tccttgtttt tcatcttcct tttcctccaa tttattgagg
     8821 taacgataaa tcgttgtacg tgaaacttgc cactgctctg caattgtctt aattggcgtg
     8881 ccattatcat aaagtgtctt aagtaagttt aaatcttttt gatttaattt ttcgggtcta
     8941 cctccatagc gtcctctagc acgtgctgca attcggccag ctgaagaacg ttctaaaatt
     9001 aaatttcgtt caaattctgc aaacgctgcg aataaatgaa ataataattg tcctgtagaa
     9061 cttgatttat ccatcgttat attttcttct aaactatgga aacttacacc tcgttcgtta
     9121 agctcattaa ctaatgtgat taaatcctcc atattacgtc ctagtctgtc gagtctccaa
     9181 acaacaatcg tatctcctga tctcgcaaat tcaattgctt tatctaatcc aggtctttta
     9241 ctttttgaac cactaatatg gtcgctaaat atcttttcac aaccatatgc gtttaatcgg
     9301 tcctcctgta aattcagatt ttgtaatcca gttgaaactc ttgcataacc tattttcaaa
     9361 atacaaacct cctatataca tatacagtgt accataactt aatttcaagg tcttttgtga
     9421 acatagattt tttgttgggt ttttgaacac gaaaatatat gtattactca cttatttcgg
     9481 cataaaaaga gagtgtacag aaaatggtta ttttctgtac actctcatct ttaataatat
     9541 attttaaatg ttattaacaa aactaattta ataagagtca agcatagttt acatatgtat
     9601 tatgtttgaa acatatctct aattactttt tactaatatc atttaaaatg tctcgcaatt
     9661 cttcaatttc tttgttattt aattcttcat ttttcgcaaa attcagcact aaacttttca
     9721 tgtcccctcc atacagttta ttaagaaagg ttttagcagt tttcatttta atatcgtctt
     9781 ctttaatatt tgatgagtaa aaataaatat tctctgattt gtatcgtttt ataatctctt
     9841 ttttatatag tcttgtgatt aatgttctaa tcgttttatc gctaacttct ttatattttt
     9901 gaatttcaac tacaatttca ttagctgata ctgatttttt accccatatt atattcataa
     9961 catcccattc agccatagat atttcaactt gcttattggc catttaaaac acccatttct
    10021 tttaatattt tttcactgat taattcagca tttttcccag atggttttcc atctgataaa
    10081 tgtgtagcaa aataatactt atcatgattt gtaattacgt aacctacaaa ccacccatta
    10141 ttatacttcc cgtttactat acctgtacct gttttcccat acagttcata cttttcattt
    10201 ttcttaatca ataatgaaga agataattga ttctttgctt ttttactaaa atggttattt
    10261 tgttccatca tatttttaaa aactattact tgttcaagat tagatatttt caaactatct
    10321 tccatccaat agcttttata acttcccaaa tttttattac cataatttaa ttgcttgagt
    10381 tgagtcgcag tatagttctt tggtatttga tcgctaatac gttcgaagta ccaattaact
    10441 gaattttgca ttgctgtatt taaatcttgt tccttattcc aagcatcaaa agggtaatgc
    10501 ttatgattcc atgacatacg tgaattttca tcatttataa tatgtcggtc aagtccaaac
    10561 atagctaaat aaattttata agttgaatta ggagaatacc tttttctact ttctttttcg
    10621 ttataaatat agtatttgtc tttcttcatg ctgtacatga cgaaagatcc actatttgaa
    10681 ccaaaaattt tacttttatc taaaatttgg taatcgttgt gtaagggctt tttataatta
    10741 taatcagtta tggattgccc catcaaaaac tggctttgaa ttaccatgag caaaaaagta
    10801 aaaatacaaa taaaaattag aattaatttg gactgttttt tcagattggc ttcttttata
    10861 ttaattaatc ttcttttgag taatgacttt ttaccattaa atgaatggct taatatattt
    10921 ttattaaaaa atggaacatt taatacggag tccataatag attccgcata agttttaaat
    10981 tcatttttgt taatattatt aaggacaaat ctatcggctt ctacttcatt gtcatgaatt
    11041 atctttcttt ttacaatatg tactaatgga ttgtaactca taataataga gaagatatta
    11101 aagataatta aatgtaaagt atctctattt ttagcatgag catattcatg caatattata
    11161 tattttagtc ttttgtcaat tacactttta aaatatgaac taggaatcaa aataatatat
    11221 ttcccatacc aaaaagttat tggagattga atagtctctg cttttcgaat cacaatattt
    11281 ttttatattg atggttgaaa agtatcgtat ctattttatt tttttcattt tcgtttagat
    11341 aaagtgactg tttctttaaa tatttaagat ataataaggc tttcaaaaat ttaaaactta
    11401 aaataataac taaaactatc caaataactg tgcagatatt atcaattgaa tcccaattaa
    11461 acttatggat atctgttgcg aactcttgaa taggtttggt ggtatttatg ttatggttca
    11521 agtcgtgtga cttactttca actgtgggcg cttgattatt cacattatta aatttaaaaa
    11581 gagagaattt aataggaatg aaaggaatta atcctgcaag aagagttaga taccaaactt
    11641 tataatttaa catataatta aaatagcgtt ttaatatgta cctaaaaaat aataacaata
    11701 gaaatataaa acaaaagctt actatgctca ttattaataa cttagccatt tcaacacctt
    11761 ctttcaaata tttataataa actattgaca ccgatattac aattgtaata ttattgattt
    11821 ataaaaatta caactgtaat atcggagggt ttattttgaa aaagttaata tttttaattg
    11881 taattgcttt agttttaagt gcatgtaatt caaacagttc acatgccaaa gagttaaatg
    11941 atttagaaaa aaaatataat gctcatattg gtgtttatgc tttagatact aaaagtggta
    12001 aggaagtaaa atttaattca gataagagat ttgcctatgc ttcaacttca aaagcgataa
    12061 atagtgctat tttgttagaa caagtacctt ataataagtt aaataaaaaa gtacatatta
    12121 acaaagatga tatagttgct tattctccta ttttagaaaa atatgtagga aaagatatca
    12181 ctttaaaagc acttattgag gcttcaatga catatagtga taatacagca aacaataaaa
    12241 ttataaaaga aatcggtgga atcaaaaaag ttaaacaacg tctaaaagaa ctaggagata
    12301 aagtaacaaa tccagttaga tatgagatag aattaaatta ctattcacca aagagcaaaa
    12361 aagatacttc aacacctgct gctttcggta agactttaaa taaacttatc gcaaatggaa
    12421 aattaagcaa agaaaacaaa aaattcttac ttgatttaat gttaaataat aaaagcggag
    12481 atactttaat taaagacggt gttccaaaag actataaggt tgctgataaa agtggtcaag
    12541 caataacata tgcttctaga aatgatgttg cttttgttta tcctaagggc caatctgaac
    12601 ctattgtttt agtcattttt acgaataaag acaataaaag tgataagcca aatgataagt
    12661 tgataagtga aaccgccaag agtgtaatga aggaatttta atattctaaa tgcataataa
    12721 atactgataa catcttatat tttgtattat attttgtatt atcgttgaca tgtataattt
    12781 tgatatcaaa aactgatttt ccctctatta ttttcgagat ttattttctt aattctcttt
    12841 aacaaactag aaatattgta tatacaaaaa attataaata atagatgaat agtttaatta
    12901 taggtgttca tcaatcgaaa aagcaacgta tcttatttaa agtgcgttgc ttttttctca
    12961 tttataaggt taaataattc tcatatatca agcaaagtga caaccataaa aggtgaagaa
    13021 cctttttata ttcaaaatca aaaaagcgga catatgaaaa gtgaaaaaat tccatctgtc
    13081 gggtttatta cagaaccaat atgctttatc taaagaacca ttatagtacg aaaaaacact
    13141 tatgattgta tgtgtacata ccctcataag tgctctattt ttgtgaatgg aatttcgctt
    13201 ccatacgata aatacgtgac cctttatccg aaaatttctt ttcatattct gttaaaatat
    13261 tactgccatc gtcttcttga tgtaaattta gatttatttt tgtaaaatac attccaaatt
    13321 gagacatact ttctaaactg taggcaaata gtcctctgtt atcagtttta aagtgtaaat
    13381 ctccttcatc atttaagatt tgttgataca acgctaaaaa cgtatgatac gttaaacgtc
    13441 gttttgcatg acgatttttt ggccatggat ctgaaaagtt caaataaata cgcgaaactt
    13501 cgccgttttt aaaatattca tttaattcaa tggcatcatt acaaataatc tttaaatttg
    13561 ttaaacccat ctctttaact ttatccaata ctttataaac gatacttttc tcacgttcca
    13621 ttgaaatata gttaatatga ggattttgag cagctaatgt tgtaataaac tgccccatac
    13681 ccgaaccaat ttcaatgtgt atcggttgcg ttttatcaaa ccattcagtc atttcccctg
    13741 catgttgacc gtccatgtca accaattcag gatgatcttt taaataatct tcagcccatg
    13801 gtttgtatcg aactctcata ttttattctc ctcttaaata aacatgttac tattcataac
    13861 ttcatttagg aatttaagcc aagtgttcat atccttatat cttttttgct cttcatacca
    13921 ttgaacaaga cctatagatt gaattaccgt ataccatttc atacgtttat ttaaattcaa
    13981 gctctcttga actccatatg tttcaagcca ttcagaccat tgttgttgtg gaacatagtt
    14041 gtaaagcagc attccgatat caattgccgg gtctgcaatc attgcacctt cccaatcgac
    14101 taaaaatagt tcatctcgat cggataataa ccaattatta tgattcacat caccatgtac
    14161 aacagtgaaa aaacgcgaat ctaaactcgg tatatgctct tctaaatagg ttaatgattt
    14221 tctcacaata tgatgtgtta aaacttctct tgataaagag gcattaattt tattaagcat
    14281 aatctcagga gtaataggtt ccatttccat acgctttaac atactcaata aaggtctaga
    14341 attgtgtatc ttctttaata aatgtgcaac tcttgtttgc ttcatttcat ttgaagatag
    14401 ttcacggcca tttttccaat gttgcgctgt aacaacctcg cctgtttcta tgcgtttcgt
    14461 ccatactaat ttgggcacaa taccttctgc tgataatgcc gcaataaatg gatttgaatt
    14521 tcgttttaaa aacaactttt gtccatcttg ttcagccata tatgcttcac cagatgcacc
    14581 acctgcagaa tcaagtgtcc atcctaattg ataaaactgc tccaactcgt ccacctcact
    14641 ttcaattaga aaatggctct agaaataggt ttttcaagag ccatatattc taatttataa
    14701 caccatactg gtacaaattt tatgtccaga taattatttt aaatcctcaa ccaatgccta
    14761 cattacacga ctaaatttaa atcgtaatgt ctgtcattga caccatacat tctatagtca
    14821 cttacttgac ataaaatgtt accgtgtcta aaactacatg tttttgtaac ttttgtaggc
    14881 gataaactct agttttcaaa ataattgcta tcccattttc atggttaaca taaatttatg
    14941 aactgtaaca tttacgtact tagtaaaata tgatgcacat catatttgta actcatagaa
    15001 aattttataa tttttatcat tatatttcaa ctgaaaatga gaaacaaaat ggcacttttt
    15061 actaatatgt gttttctaaa caacactttt aagcttcgtt ttaaattata acataattca
    15121 cttacgaaag ttgataaatt taagtaa
//
LOCUS       DAMZPP010000068        14986 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMZPP010000068 DAMZPP010000000
VERSION     DAMZPP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14986
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.068"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..317)
                     /locus_tag="POH73_001563"
     CDS             complement(<1..317)
                     /locus_tag="POH73_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDI6887513.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALAGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIET"
     gene            377..496
                     /locus_tag="POH73_001564"
     CDS             377..496
                     /locus_tag="POH73_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887514.1"
                     /translation="MWWCHDVENFDVDGGNFIFMEIGIGFITRKQKRLNDDID"
     gene            complement(579..1262)
                     /locus_tag="POH73_001565"
     CDS             complement(579..1262)
                     /locus_tag="POH73_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HDI6887515.1"
                     /translation="MKLTALAKVTLALGILTTGTLTTEAHSGHAKQNQKSVNKHDKEA
                     LHRYYTGNFKEMKNINALRHGKNNLRFKYRGMKTQVLLPGDEYRKYQQRRHTGLDVFF
                     VQERRDKHDISYTVGGVTKTNKTSGFVSTPRLNVTKEKGEDAFVKGYPYDIKKEEISL
                     KELDFKLRKHLIEKYGLYKTLSKDGRIKISLKDGSFYNLDLRTKLKFKHMGEVIDSKQ
                     IKDIEVNLK"
     gene            complement(1625..2323)
                     /locus_tag="POH73_001566"
     CDS             complement(1625..2323)
                     /locus_tag="POH73_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HDI6887516.1"
                     /translation="MKLTAIAKATLALGILTTGVMTAESQTVNAKVKLDETQRKYYIN
                     MLKDYYSQESYESTNISVKSEDYYGSNVLNFNQRNKNFKVFLIGDDRNKYKELTHGRD
                     VFAVPELIDTKGGIYSVGGITKKNVRSVFGYVSHPGLQVKKVDPKDGFSIKELFFIQK
                     EEVSLKELDFKIRKMLVEKYRLYKGASDKGRIVINMKDEKKHEIDLSEKLSFDRMFDV
                     LDSKQIKNIEVNLN"
     gene            complement(2664..3359)
                     /locus_tag="POH73_001567"
     CDS             complement(2664..3359)
                     /locus_tag="POH73_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HDI6887517.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAAEKQERVQHLHDIR
                     DLHRYYSSESFEYSNVSGKVENYNGSNVVRFNPKDQNHQLFLLGKDKEQYKEGLQGQN
                     VFVVQELIDPNGRLSTVGGVTKKNNKTSETNTPLFVNKVNGEDLDASIDSFLIQKEEI
                     SLKELDFKIRQQLVNNYGLYKGTSKYGKIIINLKDENKVEIDLGDKLQFERMGDVLNS
                     KDIRGISVTINQI"
     gene            complement(3806..4510)
                     /locus_tag="POH73_001568"
     CDS             complement(3806..4510)
                     /locus_tag="POH73_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HDI6887518.1"
                     /translation="MKMTAIAKASLALSILATGVITSTAQTVNASEHESKYENVTKDI
                     FDLRDYYSRASKELKNVTGYRYSKGGKHYLIFDKNRKFTRIQIFGKDIERIKKRKNPG
                     LDIFVVKEAENRNGTVYSYGGVTKKNQGAYYDYLSAPRFVIKKEVGAGVSVHVKRYYI
                     YKEEISLKELDFKLRQYLIQDFDLYKKFPKASKIKVTMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            complement(4871..5848)
                     /locus_tag="POH73_001569"
     CDS             complement(4871..5848)
                     /locus_tag="POH73_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HDI6887519.1"
                     /translation="MKMTQIAKTSLALSLLTTGASIVTTQSANAEVASALKTEQAIKS
                     KIQKVTTSPSTKVKATQPTPSVTTTTPPSPNVQPQSPQPTPSVTTPPSPNVQPQSPQP
                     TPNPTTPHSSNVETKQPQSPTTKQAQKEINPKYKDLRTYYTKPSLEFEKQFGFMLKPW
                     TTVRFMNVIPDWFIYKIALVGKDDKKYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITK
                     TNSKKVDHKAELSITKKDEKGKISHDDSEYKITKEEISLKELDFKLRKQLIEQHNLYG
                     NIGSGTIVIKTKNGGKYTFELHKKLQEHRMADVIDGTSIERIEVNLKSS"
     gene            complement(6195..7226)
                     /locus_tag="POH73_001570"
     CDS             complement(6195..7226)
                     /locus_tag="POH73_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HDI6887520.1"
                     /translation="MKIRTIAKASIALGLVTTGASIVTTQSANAEVASALKAGQLAKI
                     NVSTSAITTTAQAVNAEQNHTANPEQAAKSNTENVSTSLSTQTEQTPKSNIQKATQPA
                     PSGTTQSLPNAQPQSTQPTPSVTTPPSSNVETPQPTSPTTKQAQKEINPKFKGLRSYY
                     TKSSLEFKNELGIIIKKWTTIRFMNIVPDYFIYKIALVGKDDKKYGEGVHRHVDVFIV
                     LEQNKYGVDKYSVGGITKANRKKVDYKTGISITKEDKKGTISHDVSEYKITKEEISLK
                     ELDFKLRKQLIEQHNLYGNIGSGTIVIKMKNGGKYTFELHKKLQQHRMADVIDGTNID
                     RIEVNLKSS"
     gene            complement(7514..8215)
                     /locus_tag="POH73_001571"
     CDS             complement(7514..8215)
                     /locus_tag="POH73_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HDI6887521.1"
                     /translation="MKMKSIAKVSLVLGILATGVNTVTEQPVHAENKHVQVSQNSKNL
                     KAYYTQPSVEYKNVTGYISSIQPKPGTKFLNMIEGNTVNNLALVGKDKEHYHTGVHRN
                     LDIFYVNEDKRFEGAKYSIGGITKASDKVVDQVAEARVIKEDHTGEYDYDFFPFKIDK
                     EAMTLKEVDFKIRKHLIDHYGLYGEMSSGTVTVQMKYYGKYTIELDKKLQEDRMADIV
                     KVIDIDRIEVKVKKA"
     gene            complement(8498..9178)
                     /locus_tag="POH73_001572"
     CDS             complement(8498..9178)
                     /locus_tag="POH73_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HDI6887522.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNIQSVQAKTEVKQQSEADLKL
                     YYNGPSFEYKKVTGYGFIEGKDRFIDFIYNGQYNKISLVGSDKDKYNEEVNPDIDVFV
                     VREGNGRQADNHSIGGVTKTNRGVYYDYIHSPILEIKKGNEEPQNSLYQIYKENISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMKDGKSHTIDLSQKLEKERMGDSIDGRQI
                     QKILVEMK"
     gene            9650..10495
                     /locus_tag="POH73_001573"
     CDS             9650..10495
                     /locus_tag="POH73_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6887523.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVSVRQLDYFNQESMVKAFKGIDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYASRLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNEFQQLVNDQPQTLQSF
                     LQENI"
     gene            10514..10873
                     /locus_tag="POH73_001574"
     CDS             10514..10873
                     /locus_tag="POH73_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HDI6887524.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSRKTSETFNISVDKLK
                     DKNINVLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            11395..11547
                     /locus_tag="POH73_001575"
     CDS             11395..11547
                     /locus_tag="POH73_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887525.1"
                     /translation="MVPEEKGSITLSKDAATIFATAKFKPFQNRIKNNPQKTNPFLKL
                     HENKKS"
     gene            11805..12131
                     /locus_tag="POH73_001576"
     CDS             11805..12131
                     /locus_tag="POH73_001576"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDI6887526.1"
                     /translation="MTTRERRSFSSEFKLQKVRLYENGKPKNEIIREYDLTTSTFSNP
                     IKQHQNTGSFNHQDNLKSDEKELIKLRKEVQHLKMENDVLKQILLITRRNRNHLTECV
                     SIFNIH"
     gene            13276..13981
                     /locus_tag="POH73_001577"
                     /pseudo
     CDS             13276..13981
                     /locus_tag="POH73_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            14373..14909
                     /locus_tag="POH73_001578"
     CDS             14373..14909
                     /locus_tag="POH73_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887527.1"
                     /translation="MHNKNEFNKKLKDFIGSDKRMALVKGYINEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
BASE COUNT         4761 a         2517 c         2151 g         5557 t
ORIGIN      
        1 gtttcaattt cacgaatcat cgcactgaat aaatcttgtg gctcaatgaa gtaaccaact
       61 tgatcaatta attctgcttt taagtcttca cggtattctt catctgccca tgcttcttga
      121 tatgtaatat cttcacctgc caaggcatct gcatattctt gttccgcttt ttcagataag
      181 aagcgataga aaatcaagcc taaaatgtaa ttacggaatt cactcgcatc catattccct
      241 cttaaatcat tcgcaattga ccataatttt ttatgtaatt cagcttgttg ctgacgttgt
      301 ttttcagtaa tagacatgtg attcctccgc ctttgcataa gtaatttgtc tctttgtgta
      361 atagatttat tataacattt ggtggtgtca cgatgttgag aattttgatg ttgatggtgg
      421 aaattttata ttcatggaga ttgggatagg attcataaca cgcaaacaaa aacggcttaa
      481 cgacgatatc gattgatgca tccttttggt aaggtatacg caccaattta cttttcatcg
      541 ttaagccgct tcaattactt tttattattc ataatgattt actttaaatt cacttcaata
      601 tccttaattt gtttactatc tatgacttcc cccatatgtt tgaattttaa tttagttctt
      661 aaatcaaggt tataaaagct accatctttc agactaattt taatcctacc atcttttgag
      721 agtgttttat aaagaccgta tttttcaatt agatgctttc tcaacttaaa atctaactct
      781 ttcaatgata tttcttcttt tttaatatca taagggtaac ctttcacaaa agcatcttca
      841 cccttttctt ttgtaacatt taatcttggt gtgctgacaa agccggatgt tttattggtc
      901 tttgttacac caccaacagt atatgatatg tcgtgcttgt ctcttctttc ttgaacaaaa
      961 aacacatcta agcccgtatg tcttcgctgt tgatatttac ggtactcatc tccaggcaat
     1021 aatacttgag tcttcatccc tctatattta aaacgtaagt tatttttacc atgtctcaaa
     1081 gcattaatgt ttttcatttc cttaaagttt ccagtgtagt atcggtgtaa tgcttctttg
     1141 tcatgtttgt ttactgactt ttgattttgt ttagcatgac ctgaatgggc ctctgttgtt
     1201 aaagttcctg ttgttaaaat ccctaatgct aatgttactt ttgctaatgc tgtcaatttc
     1261 atatttattt actccaatct taatgtattg gattgttatt attacgtaat ttgaatcata
     1321 tacgcttatt ataacgtcca tcgtttaacg cgataattaa cctcgattta aatatgcgtg
     1381 aattattatt aattaaaaac aaatatattt taatgtccag gttaattaac tttcacgctt
     1441 tacatgtcgt tgtttttccg gatcatcgtt ttctacaact aattgtgtta tttctactct
     1501 ttgaaacgca aaaaacaact taacaacaat aatgtattta acacagttct tattcaaagg
     1561 tacgggtgtc aatgcacatt ttatcgttaa gccgcttttt aattttttat actatcatct
     1621 ttaattaatt caaattcact tcaatatttt taatctgctt actatccagc acatcaaaca
     1681 tacgatcaaa acttaatttt tcacttaaat caatttcatg tttcttttcg tctttcatat
     1741 tgataacaat tctaccttta tctgacgcgc ctttatacaa tctatatttt tcgactaaca
     1801 tttttctgat tttaaaatca agttctttca atgatacttc ttctttttga ataaagaaca
     1861 actcttttat cgaaaagcca tctttaggat caactttttt aacttgcagt cccggatgac
     1921 ttacatagcc aaacacggat ctcacatttt tctttgttat accgccaacg ctatatatgc
     1981 cgcctttagt atctattaat tcaggtactg caaagacatc acggccatgc gtcagttctt
     2041 tatatttatt tctatcgtcg ccaattagaa atactttgaa atttttattt cgttggttaa
     2101 agtttaaaac gttagaccca taataatctt cacttttaac actaatgttt gttgattcat
     2161 agctttcttg agaatagtaa tcttttagca tatttatata atatttgcgt tgtgtttcat
     2221 ccaactttac tttcgcgttt acagtttgac tttctgctgt catcacacct gttgttaata
     2281 ttcctaatgc taatgttgct ttagctattg ctgttaattt cataatttat tgctccaatc
     2341 ttaatgtatt ggattgttat gattacgtaa tttgaaacat ctgcctttat tataaagtgc
     2401 atcgaaacaa actgacatta acgtgcgttt aaataacttt gaatcattgt taatgaacac
     2461 ctaattaata ttcaaagtac gtggttttaa tgagtggtat gacttattta gtatactcgt
     2521 gacttgtgtt atcaaatgta aaaaacagcc taacgacgac gttgctttaa atacgctcct
     2581 ttgataggat atacatattt attcaacgtt tcatcgttaa gctgcctcaa aatttattac
     2641 tttaaagtca ttgattgctt actttaaatt tggttaatag tgactgatat acctctaatg
     2701 tctttactat tcaacacatc gcccatgcgc tcgaattgta atttatcacc taaatcaatt
     2761 tctactttat tttcgtcttt caaattgata atgattttac cgtatttaga tgtaccttta
     2821 tataatccgt aattattaac taattgttgt ctaattttga aatcaagctc ttttaatgag
     2881 atttcttctt tttggattaa aaatgagtca attgatgcat ctaaatcttc accattaact
     2941 ttattaacaa ataaaggtgt attagtttca gaagttttgt tgtttttctt cgttacacca
     3001 ccaacagtag atagtctgcc gtttggatca attaattctt gtactacaaa gacattttgg
     3061 ccttgtagac cttctttata ttgttcttta tcttttccta ataagaataa ttggtgattt
     3121 tgatcttttg ggttaaagcg tacaacgtta gaaccattgt agttttcaac cttaccacta
     3181 acattactat attcgaaact ttctgatgag tagtatcgat gtaaatctct aatatcatgt
     3241 aaatgttgta ctctctcttg tttttctgcc gcttgaactg cttgaccttc tgatgtaatg
     3301 acaccagtag ttaataaacc taatgctaat gttgctttag ctaacgtttt taatttcata
     3361 gtattattct cccaatctat ttttaaattt tgacttaata tatgtttatg tgattaattc
     3421 aattacgtaa tacgaacaat ctactatcat tatataagcc ggatactgcg ctatgaatta
     3481 accgcttttt aactattcct gaaccaatgt taagctaatt aatggaatcc taacagtatt
     3541 aatcattttt tataactcta tgctattttt ttcaccattc acactttctt tcaatttaat
     3601 tatgttttcc acattgtttc atgtcactag tgcgacaccg tgcgactata ggtatcaact
     3661 attttcacag tttttattgt cgttattatc tttcatcgac ccacttcatt tccgatcgta
     3721 acgacacaaa aaagaagcta agcaacctat gctgcctagc tcatctatac tatccatatt
     3781 ttactattat ccatgtttcg ttgaattatc taatattggc ttctattttc tcaatatttc
     3841 tgccatctat gacgtcactc attcggtttg tttgtaactt tttattgagt tcaaacgtat
     3901 aatagccgcc atctttcatt gtcactttta tcttgctagc tttaggaaac tttttataca
     3961 gatcaaaatc ttgaattaaa tactgtcgta atttaaaatc aagttctttc agcgaaatct
     4021 cttccttata aatgtagtat cttttaacat gcacagaaac acctgcacct acttcttttt
     4081 taataacgaa tctaggtgcg cttaagtaat catagtaagc gccttgattt ttcttagtaa
     4141 cgccaccata tgaatacact gtgccgttgc gattttccgc ttctttaact acaaatatat
     4201 ctaatcctgg atttttacgt tttttaatcc tttctatatc tttaccaaaa atttgtattc
     4261 ttgtgaactt tctattttta tcaaagataa ggtagtgctt gccacctttg ctataacgat
     4321 agccagtaac atttttaagt tccttacttg cgcgactata gtaatctctc aagtcgaaga
     4381 tatcttttgt cacattttca tattttgatt catgttcgct cgcatttaca gtttgagccg
     4441 ttgatgttat aaccccagtc gctaaaatac ttagagctaa actggctttc gcaattgctg
     4501 tcatcttcat agttgtatgc tccaatcgta attattagat ttgttcgatt acgtttattg
     4561 aatcatacag ctttattata gttggcgtat gtctctattc atattaaacc ttgtttaact
     4621 atatttgaat catcgttaat taaattaagg aatccataat gttcgttaaa taaaaatgac
     4681 ttttgttgta cttcaacgct tgatagattg gtatttttag acacatttag gacataagca
     4741 tgttatgtgt ggtcaacatc aaatattcgt cttcttatac cactgtgcaa cacgaaagaa
     4801 gctgagcaac atgtaaattt ttgtcactca acttctcggt acatcgttca acggttctat
     4861 gcagtatcca tcacgatgat tttagattca cttcaattct ttcgatacta gtgccatcta
     4921 tgacatctgc catgcgatgt tcttgtaatt ttttatgcaa ttcaaacgtg tacttcccac
     4981 cgtttttcgt tttaataaca attgttcctg aaccaatatt accgtacaaa ttatgttgtt
     5041 caatgagttg ctttctcaat ttaaaatcaa gctctttcaa ggaaatctct tctttagtta
     5101 tcttatattc tgaatcatcg tgtgagattt tgcctttctc atctttttta gtaatgctta
     5161 attctgcttt gtgatcaact tttttactat ttgtcttcgt gatacccccg acagaatatt
     5221 ttttcaattg atatttattg tcctctaaaa cgataaatac atcaatatta tcgtatggcc
     5281 catctttata tttcttatca tcttttccaa ctaaagctat tttataaatg aaccaatctg
     5341 gtataacatt cataaaccta actgtcgtcc atggtttcag cataaagcca aattgctttt
     5401 cgaattctaa actcggtttc gtataatatg ttcttaaatc cttatattta gggtttattt
     5461 ccttttgtgc ttgttttgtg gttggcgatt gcggttgttt tgtttcgaca ttagatgaat
     5521 gcggtgttgt tgggtttggt gttggttgtg gcgattgcgg ttgtacattt ggtgaaggcg
     5581 gagttgttac acttggtgtt ggttgtggcg attgcggttg tacatttggt gaaggcggag
     5641 ttgttgtagt tacgcttggt gttggttgtg ttgcttttac ttttgttgaa ggcgatgttg
     5701 ttactttttg tattttagat tttattgcct gttctgtttt aagtgctgat gctacttctg
     5761 cattagccga ttgcgttgtt actatacttg cacctgttgt taaaagtgat agtgctaaac
     5821 tggttttcgc aatttgtgtc attttcatag ttgtatgctc caatctatta tattcgattg
     5881 ttctttacgt aattagaatc atacaactac attatagaag acgtatcggt ctattcacat
     5941 taaaccatgt ttaactatat ttgaatcatc gttaagtaat ccataatttt cgttaaatac
     6001 agatggtttc gaagcgattc aacgcttgca cattgggcat tgcatcattt tcaagacatt
     6061 gagatgtcac ttttacaaac aagtgaataa tttcccttaa catcattacg caaaacaaaa
     6121 gaagctaagc aatgatgtag gtcattgtcg cttaacttct catttcatcg ttcaacggtt
     6181 ctatgtagta tccatcacga tgattttaga ttcacttcaa ttctatcaat atttgtacca
     6241 tcaataacat ctgccatgcg atgttgttgc aattttttgt gtaattcaaa cgtgtacttt
     6301 ccaccatttt tcattttaat aacgattgtt cctgaaccaa tattaccgta caaattatgt
     6361 tgttcaatga gttgttttct caatttaaaa tcaagttctt tcaaggaaat ctcttcttta
     6421 gtaatcttgt attctgaaac atcatgtgag attgtacctt ttttatcttc tttagtaata
     6481 cttattccag ttttgtaatc aactttcttt ctatttgctt ttgtgatacc accgaccgag
     6541 tatttgtcta cgccatattt attttgttct aaaacgataa atacatcgac atgcctatgc
     6601 acgccttcac catatttctt atcatctttt ccaactaaag ctattttata tatgaaataa
     6661 tctggaacaa tattcataaa tcttattgtt gtccattttt tgataattat acccaactca
     6721 tttttaaatt ctaaacttga tttcgtataa tatgatctta aacctttaaa tttagggttt
     6781 atttcctttt gtgcttgttt tgtggttggc gatgttggtt gtggtgtttc gacattagat
     6841 gaaggcggtg ttgttacact tggtgttggt tgtgttgatt gcggttgtgc atttggtaaa
     6901 gactgagttg ttccgcttgg tgctggttgt gttgcttttt gtatgttaga ttttggcgtc
     6961 tgttctgttt gagttgaaag cgatgttgat acattttctg tgttagactt tgccgcctgt
     7021 tcaggattag ccgtatgatt ttgttctgcg ttcactgctt gcgctgtcgt tgtgattgcg
     7081 cttgttgata cgtttatctt tgctaactgt cctgctttaa gtgctgatgc tacttctgca
     7141 ttggccgatt gcgttgttac tatacttgca cctgttgtta caagccctaa cgctatactc
     7201 gctttcgcaa ttgttcttat tttcatagtt gtatgctcca atcgtaatta ttcgattgtt
     7261 ctttacgtaa ttagaatcat acaacttcat tatagaggac atattggtct attcacatta
     7321 accctcgttt aacaattgtt gaaatattat taagtcattt aagcaaaaaa taatgagtac
     7381 tttcgaggtt tatgtggtgt tactatgtat ctttgaaatg attttaagac ataaaaaaga
     7441 agttaagtaa cgttttatca tcacttaacc tcactattgc aatttcaatt cattacgacg
     7501 accagtctaa gcattatgct tttttaactt tgacttcaat cctatcaata tctatgactt
     7561 tgacgatatc ggccattcgg tcttcttgta actttttatc caattcaatt gtatacttgc
     7621 catagtattt catttggaca gtaactgttc ctgaactcat ttcaccataa agaccataat
     7681 gatcaataag atgttttctt attttaaaat caacctcttt taatgtcatt gcttctttat
     7741 ctattttaaa tgggaaaaag tcataatcat attcaccagt gtgatcttct ttaatgactc
     7801 ttgcttctgc tacttggtcg acaactttat cgcttgcttt tgtgatacca ccaatcgagt
     7861 actttgcacc ttcaaatctc ttatcctcat taacataaaa tatatcaagg ttgcgatgta
     7921 cacccgtatg atagtgttcc ttatctttgc caactaaagc tagattatta actgtattac
     7981 cttctatcat attcaaaaat ttagtgcctg gtttaggttg aatactactg atataacctg
     8041 tcacattttt atattcaaca ctaggttgag tatagtacgc ttttaaattt ttactatttt
     8101 ggcttacttg aacatgttta ttttcggcat gcaccggttg ttccgttaca gtatttacac
     8161 ctgtagctaa aatacccaac actaaactta cttttgcaat tgatttcatt ttcataattg
     8221 tatgctccaa tatattagat tagattgttt tattacgtaa attgaatcat acaaccatat
     8281 tataggagct gtatgctgat attcacatta accctttttt aactattcat aaaatatgat
     8341 taagctagtt aagtaaaaga taatgcgtgc tgcgactgtt tgtgtgagtt ttgtaacttt
     8401 gccctcaaaa aagaagctaa gcaacagtac gtcgcttaac ttccatatat ttccataaca
     8461 agcacgattc aacatagcgc tttgtcgtta gaaagtatta tttcatttct actagaattt
     8521 tttgtatttg tctgccgtcg atagaatcac ccatacgttc tttttcaagt ttttgactta
     8581 aatcgatagt atgtgatttg ccatctttca tagtaattgt aatttgacct tgtttaagac
     8641 catttgaata caagccgtgt tgtttgattg cacgttctcg taatctataa tcaagttctt
     8701 ttaatgagat attttcttta taaatttgat acagactatt ttgtggttct tcattacctt
     8761 tcttaatttc aaggattgga gagtgtatat agtcgtaata cactcctcta ttagtttttg
     8821 ttacgccacc aatcgaatga ttatcagctt gtctaccgtt tccttctcta acgacaaaca
     8881 catctatatc tgggttaact tcttcattat atttatcttt atcagaacca actaaagata
     8941 ttttattata ttgtccattg tatataaaat caatgaatct atctttacct tcaataaatc
     9001 catatccagt tactttttta tattcaaaac ttggtccatt ataataaagt tttaaatcag
     9061 cctcactttg ttgtttaact tctgttttcg cttgtactga ttgtatattc gacgttatta
     9121 cacctgttgc taacattccc aatgctaaac ttgcttttgc tatcgcttta aatttcataa
     9181 tttttagccc caatctattt aaatttgatt tttcaattac gtaatatgaa caatctacct
     9241 acattatatt gaggcgctat tccctttcaa attaaacctc atttaactaa acttgaatca
     9301 ttgttaaggt aattaataaa atgctaactg ttaataatca attcttatcc gtcaagactc
     9361 ttttttatac aattcacact ttaaaccatt ttaattttaa aaactgtaaa actttcagac
     9421 acaaaaatga catcatatcg tcgcagtacc atgtcaaaat tttcaaagat catggtgaca
     9481 cgcatcaact aaaacgcgtc attacggtat ttttaacatt aataacatat gcgtatttac
     9541 tatacacggc gacaataaat acagattgca attttttata aaatcaaatg ttttgaaaat
     9601 catcgaataa gtattatgtt atgtatataa agattaggag atgaagtgaa tgaatattat
     9661 gttaacaggt gctacaggtc atttaggcac acatattaca aatcaagcca ttgcaaatca
     9721 catagatcat tttcacattg gtgttagaaa tgttgagaaa gttccagaag attggcgcgg
     9781 aaaagtttct gttcgacaat tagattattt taatcaagaa agcatggtaa aagcatttaa
     9841 aggcatcgac accgttgtgt ttattccaag tattatccat ccatcattta aacgtattcc
     9901 tgaagtggaa aatttagtat atgccgcaaa gcaaagtggc gttgctcata tcattttcat
     9961 aggttattac gcagatcagc ataataatcc attccatatg agcccttatt tcggatatgc
    10021 atcacgttta ttggcaacaa gtggcattga ttatacgtat gtaagaatgg cgatgtacat
    10081 ggatccactt aaaccatatt taccagaatt gatgaatatg cataaactga tttatccagc
    10141 tggtgatggt cgtattaatt atattactag aaatgatatt gctagaggtg tcattgctat
    10201 tattaaaaat ccagatactt ggggcaaacg ttatttatta tcaggctaca gttatgatat
    10261 gaaggaactt gctgcaattt tatctgaggc atcaggcaca gaaattaaat atgaacccgt
    10321 ttcattagag acatttgcag aaatgtacga tgaacctaaa ggctttggtg cattattggc
    10381 atcaatgtac gacgcaggag caagaggact attagaccaa gaatccaatg aattccaaca
    10441 attagtcaat gatcaaccac agacactgca atcattttta caagaaaata tttaatacta
    10501 aaggagcgtt acagtgaata tcatctcgac aattttaatc atatttgtgg cattagaatt
    10561 tttctatatt atgtaccttg aaacgattgc tacaacttcc agaaagacca gcgagacatt
    10621 taatataagc gtcgataaat tgaaagacaa aaatattaac gtacttttga agaatcaagg
    10681 cgtatataat ggtttaatcg gagttttgct aatatacggt ttatttatca gcagtaatcc
    10741 aaaagaaata tgcgcagcta ttttagtgta tatcattggc gttgctattt atggtggcct
    10801 ttcaagcaat attagtatct ttttcaaaca aggcacattg ccagtattgg cacttatatc
    10861 aatgctttgg taagtattgg tgttttgggg tttggagatg tagtcggagg tttggaggat
    10921 ttgagcgatt tgggtatgga ctttagactc agagtatttc aacctaatta tttcaagcag
    10981 aggtgacagt agcgttgcct ctgtttcctt atagaaaaat tatttaatga agaaaattca
    11041 tatctgtttt aatttttagt tgataaatac tccatatatt agaatggcta ctttatctat
    11101 tgcatcaatc ctttaaaaca aaaaacccat gatttcgaaa ttcccgtatg gtggggttcc
    11161 tactctcatg ggtcagttaa ctaaattatc actatcagtt tataatttca atattattaa
    11221 caatatatgt agtcgcaaaa ggaatgagga catgaggact ttggtgttga ttggcattac
    11281 ataacgcttc caaacaaatt attcggtaac aataagaaac tatttacaca aattattata
    11341 catagtaaaa ttattttata aactttaaat caaattgtac aaggagtgaa tatcatggta
    11401 ccagaagaaa aaggttctat tactttgtca aaagatgcag ctaccatatt tgcaactgca
    11461 aaattcaaac catttcagaa cagaattaaa aataacccac aaaaaacaaa tccatttctt
    11521 aaattacatg aaaacaaaaa atcttaatca ctttatttat agcatttctg atctcagaaa
    11581 tttaataaag gacaacgccc tctttcataa ctatataagt caagaaacca agaaattcca
    11641 aacatcttta aacccttcaa tagcacattt cctaaaacat aaagcaataa caatgaacga
    11701 tcaaaattcc taacgtactt atgatgtgtc agtgaatcgt ctacacttaa ttgaacaaat
    11761 tctatgagaa tagatattgt taatttaaga aagtaggcta ttttattacg acaagagaaa
    11821 gaagatcatt tagttcagag tttaagttac aaaaggttag attatatgaa aatggtaagc
    11881 ctaaaaatga aattatacgc gagtatgatt taacaacctc aactttctca aatccgataa
    11941 aacaacacca aaatactggg tccttcaatc atcaagataa tttaaaaagt gatgaaaaag
    12001 agttaataaa attacgcaaa gaagttcaac atttaaaaat ggaaaatgat gttttaaagc
    12061 aaatattgct aatcacgaga cgaaatagaa atcatttaac agaatgcgta tcaatattca
    12121 atatccatta atgtgcaaag tcttagaagt acttattatg aatcaataaa aaggaataat
    12181 aagagcaaaa aacatgctga attggataat cgttcactcc cttaacaaat atatgtactt
    12241 ttgaataact tgtaatgtaa atctacttga ctgatataaa ccttcatcca ctttatgaac
    12301 aattttattt actaaagttt tttatattta cgataatctt ttacgtttat cttcttattc
    12361 aacggtatct acaaaaacac tctatacgtt tcaattgtat gattgaatgt attatagtgt
    12421 gcgaaaacta tgcaacactg ctttaaactg atctgaacgc atcatgtatc tgctttaaat
    12481 catttacttc atcataataa aatacaatta catatatata atatttttat aattgcagtg
    12541 ctttttgatt aattcgccat gattattttc acagacttta atatcaccat acacagcaac
    12601 accactgaaa catgtatatc gcattttaat ttaatttcat ccattttttg tgcctctaat
    12661 ataacaattt ttgtgcgtca tttaattcgg tcgcaaaact tatatcagaa gatatttttt
    12721 ctatttcttg aaacttttgt ataagttcat caaattcttt taaatgtact aaatgattta
    12781 attaaatata gtgtatatat atggcagcaa caaaattgat tgtgaaacta tttatgaaat
    12841 aaaaaatttt ctaacttcaa atattagagt tgatttagaa actgaacttt ttaaagacac
    12901 attacttgaa gattctaatt tatctgatta tataaagaac caacttccaa cgatatggag
    12961 agactaaaaa atagtttaaa tttatttaat ttcaataaaa actcataata atatttaagt
    13021 aagttagatt gcttttagca ttaggaattc gtttataatt agatgttcaa tataggcttt
    13081 tttacacata catgaactat aaaaagcgga taaataggag gtcattgcga aatttgttaa
    13141 atacaaaatt gatgagttcc acctaataaa attgttggtt ttatagtaac atctattttc
    13201 gtaccattca tgttaatttc agtgctaata atgctgatat aaagaagaat aaattgatta
    13261 ggagaacact atttaatgtc taaaaatatc aatcaagcaa atagtaaatt aaatacgtca
    13321 aacaaaaaac taaaacaagc ttattcaaaa tccgatagta aaaacttgaa agttatatac
    13381 atgcctcatt ggctagagtt ttattcaatt gctatacata gcgatgttac aagcaataaa
    13441 aaaagatatt ataaatcata tagtagagga acagtggtat atgtaaaact agggagtaat
    13501 attggaagtg agttttctgg taatcatttt tgtgtaatat tagataacaa agacaataaa
    13561 ggaaaagaaa cagttacaat agttccactt tcttccaaag gtaataaaaa ttatttgaag
    13621 ttaaacgaat cggtgctaaa tctaacaaca acagatttaa aaaaaacaga ttatcgatat
    13681 cagtagtaaa gtacaagcat tagcccaaaa atatagagat attgacatta aacttagcac
    13741 agaagataaa aagcttttgt ctaacttaaa tcaaaaagct aatgaactgt atagagtgat
    13801 aggtgtttat tccaagcaca aagggaaaga tacatacgtt aatatatcag caattacaac
    13861 aataagcaaa agacgtataa gcaaaataaa tgattctgat cctactggaa cgataataat
    13921 ttcagaagat gacatgaatg aaatcgaaaa gcaattaaag ataaagttct tttctaattg
    13981 aattgtaata ttacatctga tataattggt ttacaaattc ccggtaacca ttccggcttc
    14041 attctcttga agaatgacat attctcagcg ttttagctga aggtcagatg atacgtcatc
    14101 tggcctcttt ttttatataa atgcccttta agtgaaatat acaacaatct gcaaccaata
    14161 tagacatcac aaaacacaca ataaattaca caatgtgtta taagccacat gtcactatta
    14221 tcgtagctag ctgcgtcact caaaacgaat acggaacgcg ttggttattc caatcataat
    14281 attactctgc aaatacacag ccctgtaacc gagtaaatgg atagagattt gaacaaatga
    14341 aaacaatcaa ctaatggaaa ggataaaata ttatgcacaa caaaaatgaa ttcaacaaaa
    14401 aactgaaaga ttttataggt agcgataaac ggatggcttt agtaaagggt tatattaacg
    14461 agtataaatt aacaactgtt ttaagagctt taaataattc cgattacaca aaagggacga
    14521 tacacactcg ttcaataggc aacctaaaac aaatagtaga taatcgaatt attcctaaaa
    14581 atataactca aaacacaatc ttcaaattga gtaatttaac cttagaggtt aacctctatg
    14641 aaagaaacac aatttatcac ggagactttg ctatttacta tcctgtagaa tctgctctaa
    14701 taatagaaac agatactcct aggctaatta atcatataaa taacaaccca gtccaaaaaa
    14761 tatttataat aacaacaaat gactggagct ttaatacact tgaattagaa aaaatagtag
    14821 acgaaactat aatttatgat ttaaaacaag aagatcctaa acaatatgaa attttatata
    14881 aaaataaaca cggaaaattg ccttattgat gaattaaaac gcaaacttag aacaccttac
    14941 cccttttccg tccttattgt aattttaaat catcaagagg ctggga
//
LOCUS       DAMZPP010000069        14944 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMZPP010000069 DAMZPP010000000
VERSION     DAMZPP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14944
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.069"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..1038)
                     /locus_tag="POH73_001579"
     CDS             complement(<1..1038)
                     /locus_tag="POH73_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDI6887528.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTIKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSKIQEHVMHKMDKL
                     LQGLAIANDIEYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLT"
     gene            1209..2396
                     /locus_tag="POH73_001580"
     CDS             1209..2396
                     /locus_tag="POH73_001580"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDI6887529.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMASTELHDKLWDNAQYLKNGLSKLGYDTGESETPITPVIIGEEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     NLI"
     gene            2672..3550
                     /gene="hchA"
                     /locus_tag="POH73_001581"
     CDS             2672..3550
                     /gene="hchA"
                     /locus_tag="POH73_001581"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDI6887530.1"
                     /translation="MSQDVNKLSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPAKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALENDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQSLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            3708..5345
                     /locus_tag="POH73_001582"
     CDS             3708..5345
                     /locus_tag="POH73_001582"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDI6887531.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIEGELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPVNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KVDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKDTMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAANRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYTNVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            5559..6524
                     /locus_tag="POH73_001583"
     CDS             5559..6524
                     /locus_tag="POH73_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDI6887532.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFTRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IDAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPDFKLEYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLVSMTKLMLEAIEQKDTVKNNN"
     gene            6859..7935
                     /locus_tag="POH73_001584"
     CDS             6859..7935
                     /locus_tag="POH73_001584"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDI6887533.1"
                     /translation="MSQAVKIELRETLKEKPDTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKKDGEVALFRPDENFKRLNNSLARLE
                     MPQVNEGELLEGLKQLVDLEREWVPEGEGQSLYIRPFVFATEGVLGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            8186..8869
                     /locus_tag="POH73_001585"
     CDS             8186..8869
                     /locus_tag="POH73_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6887534.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALI"
     gene            complement(8985..9647)
                     /locus_tag="POH73_001586"
     CDS             complement(8985..9647)
                     /locus_tag="POH73_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDI6887535.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMIDKIARIIQTYRQVD
                     TR"
     gene            complement(9640..10257)
                     /locus_tag="POH73_001587"
     CDS             complement(9640..10257)
                     /locus_tag="POH73_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDI6887536.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            10324..10794
                     /locus_tag="POH73_001588"
     CDS             10324..10794
                     /locus_tag="POH73_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDI6887537.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEDCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            10941..11810
                     /locus_tag="POH73_001589"
     CDS             10941..11810
                     /locus_tag="POH73_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI6887538.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKDVMAIGDNLNDVSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            11830..12396
                     /locus_tag="POH73_001590"
     CDS             11830..12396
                     /locus_tag="POH73_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDI6887539.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
     gene            12827..>14944
                     /locus_tag="POH73_001591"
     CDS             12827..>14944
                     /locus_tag="POH73_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HDI6887540.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNEATAPSENKTTEKVDSRQQNNVEQSTTSNQPKVNESDNT
                     SVKETTEEPQNTTSTQPTKKNNDATANKDNLAAQNISTQANDVSATPKTTTIKPRTLN
                     RMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHVNSTTGKTEFWATSSDVLKLKANYT
                     IDDSVKEGDTFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDSTTNTTTYTFT
                     NYVDQYTNVSGSFEQVAFAKRENATTDKTAYKMEVTLGNDAYSEEIIVDYGNKKAQPL
                     ISSTNYINNEDLSRNMTVYVNQPKNTYTKETFVSTLTGYKFNPDAKNFKIYEVTDQNQ
                     FVDSFTPDTSKLIDVTDKFKITYSNDNKTATVDLMNGQTNSNKQYIIQQVAYPDNTST
                     DNGKIDYTLDTDKTKYSWSNSYSSVNGSSTANGDQKKYNLGDYVWEDTNKDGKQDANE
                     KGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLGNGTYSVEFSTLAGYTPTTVNAGT
                     DDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKG
                     VKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLKQTGTNTTEDDKDA
                     DGGEVDVTITDHDDFTLDNGYFEE"
BASE COUNT         5528 a         2076 c         2731 g         4609 t
ORIGIN      
        1 agtcagaaag aatgcacctg gtcgtacttt caaataatgt gaaaaatctt ctccaatcat
       61 cattaaatct gattcattaa agcgtacatg taagtcattt gttgcttctt taataacttg
      121 atatgctttc tcgttgttat ggacaggcaa gtatccttta atataattca agtcatattc
      181 aatgtcattt gcaattgcca aaccttgtag tagcttatcc attttatgca tgacatgttc
      241 ttgtattttc gagtcaaatg tacgaactgt acctttacaa aatgcttgat caggaataac
      301 gctatctgtg gtgcctgctt gaatcattcc aaatgaaagt acagcttgtt taactggatc
      361 aatcgtacgt gaaattattt tttgtgcact taaaatgaac tctgccatga ttactattgg
      421 gtcaatggtt tcatgaggtt tggcaccatg accgccacga cctttaattg tgacactaaa
      481 ttcatctgga gaggccatga ttgcccccgc acgcgaatga atagttccag taggataacc
      541 actccataaa tgtgtaccgt aaattctatc tacattttcc agacatccag catcaatcat
      601 ttcttgagaa ccacctggca tgatttcttc accgtactgg aatattaata caacattacc
      661 ttctaataaa tgtttatgtt catctaaaat ttctgctacg gtaagtaaaa ttgctgtatg
      721 accatcatgc ccacacgcat gcatacatcc tggattttta gacttataag gcacatcgtt
      781 taattcctcg acaggtaacg catcaaagtc agctcttaat gcaatggtag gtcctgtgcc
      841 caaaccttta aatgtggctt tgataccatt gcggccgata ggagtttcaa tatcacaaga
      901 taactggctt aattggttaa caatataatc atgtgtttga aattcttcaa aagataactc
      961 aggatattgg tgtaaataac gtctgagttg aattgtttta ttttctttat tatttgctag
     1021 ttgaaaccaa tctaacaccc taatcactac tttctaaaat attgtttata gtataacatt
     1081 ttatgaaatt atcgtactaa atgattgctt tgagatattt tatctatgaa tgataaggct
     1141 ttcaagttat gtagaattac tgtatgataa aggtattacc aaacaatact taagggggat
     1201 tatatactgt ggttcaatca ttacatgagt ttttagagga aaatataaat tatctaaaag
     1261 aaaatggttt gtataatgaa atagatacaa ttgaaggtgc aaacggacca gaaatcaaaa
     1321 tcaatgggaa atcatacatt aacttatctt caaataatta tttaggacta gcaacaaatg
     1381 aagatttgaa atcagctgca aaagcagcta ttgatacaca tggtgtaggt gcaggcgctg
     1441 ttcgtacaat caatggtaca ttagatttac acgacgaatt agaagaaaca ctagcaaaat
     1501 ttaaaggaac agaagctgca atagcttatc aatcaggatt taattgtaat atggctgcta
     1561 tttcggctgt tatgaataaa aatgatgcta ttttatcaga tgagcttaat catgcatcaa
     1621 ttattgacgg atgtcgctta tcaaaagcta aaattattcg agttaaccat tcagacatgg
     1681 atgatttacg tgcgaaagca aaagaagcag ttgaatcagg tcaatacaat aaagtgatgt
     1741 atatcactga tggcgttttt agtatggatg gtgatgtggc taaattacct gaaattgtag
     1801 aaattgcaga agaatttggt ttattaactt atgttgacga cgctcatggt tcaggtgtta
     1861 tgggcaaagg cgctggtacg gttaaacatt ttggtttaca agataaaatc gatttccaaa
     1921 ttggtacgct ttctaaagca attggtgtcg ttggcggtta tgtagcaggt acaaaagagt
     1981 taatagattg gttaaaagca caatcacgac cattcttatt ctctacatca ttagcacctg
     2041 gggatactaa agcaatcact gaagcagtta aaaagttaat ggcttcaact gaattacatg
     2101 ataaattatg ggacaatgca caatatttaa aaaacggatt gtcaaaatta ggatatgata
     2161 caggagagtc agaaactccg attacaccag taattattgg tgaagaaaaa acaactcaag
     2221 aatttagtaa gcgtttaaaa gacgaaggtg tctatgtgaa atctatcgtt ttcccaacag
     2281 tgccaagagg tacaggacgt gtaagaaata tgcctacagc tgcacataca aaagacatgt
     2341 tagacgaagc aattgcggct tatgaaaaag taggaaaaga aatgaatttg atttaattta
     2401 atatatattt attcccacgg caaatatcgt cgtgggtttt ttcttttata aatgcttagt
     2461 ttattaacag taagttctta taacaatgtt tagtgttgtc aaccattaaa gtttgaattt
     2521 taaaagcatc ttgtagaatt tagttgtatt tttacgatta aattcatttt gattattttt
     2581 gataataagc attttaatag taatacatgt ttatagtgtg tagcatatgt ctatactagt
     2641 agtaactata tagagaaagt aggaataaac tatgtcacaa gatgtaaata aattaagtaa
     2701 gcaaccaacg ccagataaag cagaagataa cgcatttttc ccatcaccat attcccttag
     2761 tcaatataca gcacctaaaa cagattttga tggtgttgaa cacaaaggtg cctataaaga
     2821 tggtaaatgg aaagtattga tgattgctgc tgaagagaga tatgtattat tggaaaatgg
     2881 aaaaatgttc tctacgggta atcatcctgt tgaaatgtta ttacctttac atcatttaat
     2941 ggaagcaggt tttgacgttg atgttgcgac attatctggt tatccagcta aattagaatt
     3001 atgggctatg ccaactgaag acgaggcagt tataagcact tataataaat tgaaagaaaa
     3061 attaaaacag ccaaaaaaat tagcagatgt gattaaaaat gaattaggac ctgattcaga
     3121 ctatttatct gtctttatcc caggcgggca tgctgcagtt gttggtattt ctgaaagtga
     3181 ggacgttcaa caaacattag attgggcatt agaaaatgac cgctttatag ttacattatg
     3241 tcatggacca gccgcactac tttcagcagg tcttaacaga gaaaaatctc cattagaagg
     3301 atactctgtt tgtgttttcc ctgactcatt agatgaaggt gcaaatattg aaataggtta
     3361 tttacctgga cgtttgaaat ggttagttgc tgatttatta actaaacaaa gtttaaaagt
     3421 agttaacgac gatatgacag gaagaacgtt aaaagatcgt aaattattaa caggtgacag
     3481 tcctttagct tcaaatgagt taggaaaatt agcagttaat gaaatgttaa atgcaataca
     3541 aaataaataa ttaaatatta attagaggag ccccatatgt aaatgtatga gggctctttt
     3601 tttggcaaaa ttttagtgat acttgtaaaa tagaacctat tatgagtatg atttaagaaa
     3661 acgcttgcaa aactaataac tgcaactagc gatatggagg aaacatgatg tcttatagca
     3721 ttggaattga ttatggaact gcttcaggcc gtgtgttttt aattaataca actaacggtc
     3781 aagtagtatc aaaatttgtg aaaccatata cacatggtgt cattgagggt gaattaaatg
     3841 gtttaaaaat accacataca tatgcacttc aaaatagtaa tgattattta gaaattatgg
     3901 aagaaggaat atcatatata gtacgtgaat caaaaataga tccagtcaat atagtaggta
     3961 ttggtataga ctttacttca tctactatta tttttactga cgaaaacctt aacccggtac
     4021 ataatttaaa acaatttaaa aacaatccac atgcgtatgt gaaactttgg aaacatcatg
     4081 gtgcatataa agaagcagag aaattatatc aaactgctat tgaaaataat aataagtggt
     4141 taggccatta tggatataat gttagtagtg aatggatgat tcccaaaata atggaagtca
     4201 tgaatcgagc accagaaatt atggaaaaaa cggcttatat tatggaagcg ggcgattgga
     4261 ttgtaaataa attaactaat aaaaatgtac ggtcgaattg tggattaggt ttcaaagcat
     4321 tttgggaaga agaaacaggg tttcattatg atttgtttga taaagtagac cccaaattat
     4381 caaaagtaat tcaagataaa gtatctgcac cggttgttaa tattggtgaa gcagtaggga
     4441 aactggatga taaaatggca cagaaattag gattatcaaa agacactatg gtaagtcctt
     4501 ttattattga tgcccatgct agtttattag gtattggatc tgaaaaagat aaagaaatga
     4561 ctatggtgat gggaacaagc acatgccatc ttatgttaaa tgaaaagcaa catcaagtgc
     4621 caggtatatc aggttctgta aaaggagcaa ttattccaga attatttgct tatgaagcgg
     4681 ggcaatcagc agtaggtgat ttgtttgagt atgtcgctaa gcaagcacca aagtcatatg
     4741 tagatgaagc agcaaataga aatatgactg tatttgaatt aatgaatgaa aagataaaac
     4801 atcaaatgcc aggtgaaagt gggctcattg ctcttgattg gcataatgga aatcgaagtg
     4861 tattaagtga tagcaattta acaggttgta tctttggatt aactttacaa actaagcatg
     4921 aggatattta tagagcatat ttagaagcta cagcatttgg tactaagatg attatgcaac
     4981 agtatcaaga ttggcatatg gaagtagaaa aggtatttgc atgtggcggt atacctaaaa
     5041 agaatgctgt tatgatggat atctatacga atgtactgaa taaaaaacta attgttatgg
     5101 atagtgagta tgcaccagca ataggagcag caatattagg tgcagtcagt ggtggcgcac
     5161 ataattcaat taatgatgca gttgatgcta tgaaagagcc aattttatac gaaattaatc
     5221 cagaagcgga aaaagtacaa aggtatgaaa cattatttaa agcttataag gctttacatg
     5281 atatccatgg ttataaaaaa gctaatataa tgaaagatat ccagagttta agagttgagg
     5341 gataaaaaat ttaatttgac agtaattagc aatattaaac gctataaatt actaaaatca
     5401 attaatacag ctagaatttc catttacaga aatatcagca aaatgtacat gtcatatata
     5461 caataaagcg cttttatatt ataattatta tgaaaatgaa tattatgtcc tcttgtgatg
     5521 gactatggtt atatagaaga gacaaaggag atatttctat gaaaaaaatt atgattactg
     5581 gtgcattagg acaaattggt acagaattag ttgttaagtg cagagaaatt tatgggacag
     5641 ataatgttct tgctacagat attagggaac ctgaagcaga ctcacctgta caaaatggac
     5701 catttgaaat cttagacgta acagatcgtg accgtatgtt tgagttagtt agggactttg
     5761 aagcggatag tctaatgcat atggcagcat tattatcagc aactgctgag aaaaatccaa
     5821 ttcttgcttg ggatttaaat atgggtggat taatgaatgc attagaagct gcaagaactt
     5881 ataatttgca ctttttcaca ccaagttcaa ttggtgcatt tggagactca actcctaaag
     5941 ttaatacgcc acaagtaacg attcagcaac ctacgacaat gtatggtgta aataaagtag
     6001 ctggcgaatt gttgtgtcaa tattatttca cacgatttgg tgtagataca agaagtgtta
     6061 gattccctgg tttaatctcg catgttaaag aaccgggtgg tggcacaacg gactacgcag
     6121 ttgaaattta cttcaaggct gtaagagaag gacattatac aagcttcata gataaaggga
     6181 catatatgga tatgatgtat atggatgatg cgattgatgc aattattaaa cttatggaag
     6241 cagacgatgc gaaattagaa acaaggaatg gttataattt aagtgcaatg agctttgatc
     6301 cagaaatggt taaagaagca atacaagaat attacccaga tttcaaatta gaatatgatg
     6361 ttgaccctat tagacaaggt atcgctaata gttggccgga ttctattgat acaagctgtt
     6421 cacgtggcga atggggattt gatcctaaat atgatttagt gagcatgact aaattaatgt
     6481 tagaagctat tgaacaaaaa gatactgtta aaaataataa ctaatcattt ccattcactt
     6541 taatacacgg aatgatattt taaattactc tttattttaa taaactagtg catgaattct
     6601 aatattatta attatacata ttgaattcgc gagcttagtt catttttaaa taaagagttt
     6661 tttgtattaa aaagatataa ttcaaacaca gattacttgt ctctatgaaa ataatacgtt
     6721 tatactaaaa gatataggct ttctgaaaaa gtgttaatta atttcgaatt tttagaaaat
     6781 ttattgaaca gtaaaatagg gattgttata atttatgtta aacaaattct tatactatat
     6841 tattaggagg caatcaccat gtcacaagca gttaaaattg aattacgcga aacattaaaa
     6901 gaaaaaccag atacatctca attaggattt ggtaagtatt ttacagatta tatgttgagt
     6961 tatgattatg atgcggataa aggatggcat gatttgaaga ttgtacctta tggtccaatt
     7021 gaaatttcgc cagcagcaca aggtgttcac tatggacaat cagtgttcga aggtttaaaa
     7081 gcatataaaa aagatggaga ggttgcactt ttccgtccag atgaaaactt taagcgtctt
     7141 aataactcgt tagcacgctt agaaatgcct caagtcaatg aaggtgaatt gctagaagga
     7201 cttaagcaat tagttgattt agagagagaa tgggttccgg aaggtgaagg tcaatcatta
     7261 tatattcgtc cttttgtttt tgcgacagaa ggggtacttg gcgttggtgc atcacatcag
     7321 tataaattat taattatttt atctccttca ggtgcatatt atggtggtga aactttaaaa
     7381 ccaactaaaa tctatgtaga agatgaatat gtacgtgctg ttcgtggcgg tgtaggcttt
     7441 gcgaaagttg caggtaacta tgcggcaagt ttattagcac aaactaatgc aaataaatta
     7501 ggttatgacc aagtattatg gcttgatggt gttgaacaga aatatatcga agaagttggt
     7561 agcatgaaca ttttcttcgt tgaaaatggc aaagtaatta caccagagtt gaatggcagt
     7621 attctacctg gtattacacg taaatctatt atcgaattag ctaaaaactt aggatatgaa
     7681 gtcgaagagc gccgcgtttc aatcgatgaa ttattcgaat catatgataa aggtgagtta
     7741 acagaagtat ttggtagtgg tactgcagca gttatttcac ctgtaggtac attgagatat
     7801 gaagatcgtg aaatcgttat taataataat gagactggtg aaattactca aaaattatac
     7861 gacgtctata ctggtattca aaatggtact ttagaagata aaaatggttg gagagtcgtt
     7921 gtaccaaaat attaataaaa attgaatatg atcatgaaaa caaaatgtaa aagttaatca
     7981 tgactaaaat ccctttcaat gaagcgaagc atggtaataa attaagttta caatgtttat
     8041 tgttaagtat tgaaggggat ttcacttatt attatattta attcaatatt taaaaagaac
     8101 aatactacga gttcattttc aggggaataa cgaacatcat agaatcagca taatacgaaa
     8161 taaataaata ggagtattga tattaatgga atggatatta tttgataaag atggtacgtt
     8221 aattgaattt gatagaagtt gggaaaaaat aggggtacga tttgtacaat cattgcttga
     8281 gacttttcca gtacataata aagaagctgc tttaagacaa ctcggtgtca ttaaagaatc
     8341 tattgatcca aaatcagtga tgggttcagg atctttacaa caaattattc aggcatttaa
     8401 tgatgtgacg ggacaagata caaccgactg gtccaagtca acaagtcaaa agttggtaga
     8461 tgaacgtatt cctgaaatta attgggtaga aggtgttaaa gaagcactta tcgatttgaa
     8521 agcaaaaggc tatcaactag gtattgttac gagtgatact aaaaaaggtg tagaacaatt
     8581 tttagcacat accaatgcta cctcgttgtt cgatttgatc atttctaccg aagcggatgc
     8641 ctatgagaag ccaaatccta aagtattatc gcctttattt gagcaatata atgtagatcc
     8701 tcagaaagta gctatagtag gagacactgc taatgatatg aaaaccgcta gtaatgcaaa
     8761 tttaggtatg gcaataggtg tattaacagg tattgcaaca aaagaagaat tacatgaagc
     8821 tgatattatt ttaaatagtg cggcagatat tttagaagct ttaatttaaa aagtaagaca
     8881 tagtgtatat gtgaatcaat gtaaccaata gaattgataa tgtaatcgat gatatctatg
     8941 gaatatacat tgaatataaa catatacgct atgtctttag tcttttatcg tgtatctact
     9001 tgtcgatatg tttgaataat tcgagcaatt ttgtctatca taggatttaa agattcgggg
     9061 tccttatgga tatcatattc attaatattg atacgtacca caggacatgc attaaagcta
     9121 ttaatccaat cgtcatagcg tttaaatagc tttttccagt attcagggtc tgtattaatt
     9181 tccatttcgc gaccgcgttc aataatacga tcaatgacct catcatagtt acattctaaa
     9241 taaatcatta catcaggttt aggaaaataa ggtgtcatga ccatggcatt aaataagtct
     9301 gaatatgttt tgaaatcctc tttactcatt gtgccttctt cttcatgcat ttttgcaaaa
     9361 atatcaacat cttcataaat tgatcgatct tggacaaagc caccaccata ttcaaacata
     9421 cgcttttgtt ctttaaagcg ttcagctaag aaataaattt gtaaatggaa actccaacgt
     9481 tcaaaatcgc tgtaaaattt atctaaatat ggattatgtt cgacattttc aaaagacgtt
     9541 ttaaagttta atttatctgc aagtgcttgc gttagtgttg atttcccaac accaactgta
     9601 cctgcaatgg ttataatggc attttgtgga ataccgtaat tattcattgg taatatctcc
     9661 tatcataggt aatataatat gtaatatatc ttcgtaatct tgttcatttt ttagaaaatc
     9721 aatagaagtt gtatcgatta aaactacatt tgaaccatta ctttgtaagg actcataata
     9781 ctcacgataa tcttttttta actttaacag atattcatct tctatttgat gctcaaaact
     9841 acggttacgt ttagcaattc tagattttaa cacatcaagg tctgcatcta aaaagataat
     9901 catattcggc ataatcatat cttcagttaa aatatcataa attttactga atttctgaaa
     9961 ttcaacagaa ctcaaagtat ttttagcaaa tatcttattt ttatgtatat gataatcact
    10021 aactacacct tgatttagtt gtgttacatc ttgaaattgc ttatatctat tgcataaaaa
    10081 gaacatttca gtttgaaaac tccatttaga gatatcttca taaaagtctg ataaaaatgg
    10141 attttctgtg atgatttctt tttcttcata aaaatctaaa gtttgactta atttgtgtgc
    10201 aagtgaagat ttacctacgc caataggacc ttcaattgct ataaaaggtt tgttcatatt
    10261 cacatctcca aatgttaaaa tagacattga gtattgtacc ataagtgagg tatctggaca
    10321 taaatgacaa atgatatata ttttatgaca ttagcgattg aagaagctaa aaaggcagct
    10381 caactaggcg aagtacctat aggtgctatc atcactaaag atgatgaagt tatcgctaga
    10441 gcacataatt taagagaaac actacaacaa ccaacggcgc atgctgaaca tattgcaatt
    10501 gaacgtgcag ccaaagtgtt aggtagttgg cgtttagaag attgcacatt atatgtaacc
    10561 ttagaaccat gtgtcatgtg cgcaggaaca attgtaatga gtcgcattcc aagagtcgtc
    10621 tatggcgcag atgatcctaa aggtggttgt agtggcagtt taatgaattt attgcaacaa
    10681 tctaatttta atcatcgtgc aattgttgat aaaggtgtac ttaaagaagc atgtagcaca
    10741 ttattaacaa cattttttaa aaacttaaga gccaataaga aatccaccaa ttagacgata
    10801 gtacatacta attaaaattt gttagaatta cattatatga taaattaatg acaatataaa
    10861 tgttaataga aaagtatgtg cactgagtat atattcttat gtaagaacaa gattttaaat
    10921 ttacgaaacg aggtaacata atgataaaac taatagccac tgatatggat ggcacgctac
    10981 ttaatgcagc acatgaaatt tctcaaccta atattgatgc gattaaatac gctcaagaac
    11041 aagggataac ggttgttatc gcgacaggtc gagcatttta tgaagcacaa gcaccagttg
    11101 ctgacacaga tttaacagta ccatatattt gtttgaatgg tgctgaagta cgtgatgaaa
    11161 ctttcaatgt aatgagcact tcacacctta ataaatcgtt agtacacaaa attacaaatg
    11221 ttttaaaaga tgcaggtatt tattatcaag tatacacgag tcgtgcgatt tatactgaag
    11281 atccacaaag agatttagac atttacatag atattgctga gcgtgcaggt caacacgcaa
    11341 acgttgagcg tattaaaaat ggtattcaaa gacgcataga taatggtacg ttgaaagttg
    11401 ttgataatta tgatgctatt gaaaacatac ctggtgaatt aattatgaaa atattagcat
    11461 ttgatggaaa tttagaaaaa attgacaaag ctagtaaaat tttagctgaa tctccgaatt
    11521 tagctatatc atcatcttcg agaggaaata tagaaataac gcattcagat gcacaaaaag
    11581 gcattgcgct agaaacaatt gccgaaagat tagggattga aatgaaagat gtcatggcaa
    11641 taggtgacaa tttaaatgac gtatcaatgt tagagaaagt tggctatcca gttgcgatgg
    11701 aaaatggtgc tgaagaagtt aaaaaaatag cgaaatatgt cacagatacg aatgaaaata
    11761 gtggtgttgg aaaagctatt atgaaattat tacgtgaaca acaagtttaa taaaaaagag
    11821 gggtcaaata tgaaaggatt aattattatt ggcagtgcac aagtgaattc acatacaagt
    11881 gcactagcaa gatacttaac tgagcatttt aaaacacatg atattgaagc ggaaatattc
    11941 gatttagcag aaaaaccgtt aaatcaatta gatttttcag gaacaacacc gtctattgat
    12001 gaaatcaaac aaaatatgaa agatttaaaa gagaaagcaa tggcggcgga ctttttaata
    12061 ttaggaacgc caaactatca tggttcatat tctggaatat tgaaaaatgc attagatcat
    12121 ctaaatatgg attattttaa aatgaaacct gtaggcttaa taggaaatag tggtggaatt
    12181 gttagttcag agccattgtc acatttaaga gtaatcgtca gaagtttact aggcattgca
    12241 gtaccaactc aaatagcaac acatgattct gattttgcta aaaatgaaga tggttcatat
    12301 tacttaaatg atagtgaatt ccaattacga gcaagattat ttgtcgatca aattgtatct
    12361 tttgtgaata atagcccata tgaacattta aaataaatta aaaaatatgt aaatatacat
    12421 taaaaatagg tatgtagctt aaggtatttc attgagaatg cttttaagtc acatgcctat
    12481 ttttttgttc taatgcacca atagtaagcc tttgagttta ctattgtgag caatgttatt
    12541 aaataaaata agatgtaata gcgaattgaa aacaagctta gattaaaagg aaagtttaaa
    12601 aatatagata aataatttaa aacaatggaa gaaaaaaagt attaaaaatt gcaattctta
    12661 atctgaaatg catattaata tttactataa ataacattgt aaaataaaag aaaaaaatta
    12721 aaacattgta ctttatcgaa aagtgtatta agatagaaag gcagaaattt ttatatgcaa
    12781 aatattatat ttatcaaatt ttgatatttt aaaggagtat tattaaatga ataataaaaa
    12841 gacagcaaca aatagaaaag gcatgatacc aaatcgatta aacaaatttt cgataagaaa
    12901 gtattctgta ggtactgctt caattttagt ggggacaaca ttgatttttg ggttaagtgg
    12961 tcatgaagct aaagcggcag aacatacgaa tggagaatta aatcaatcaa aaaatgaagc
    13021 gacagctcca agtgagaata aaacaactga aaaagttgat agtcgtcaac aaaataatgt
    13081 tgaacaaagt acaacgtcaa accaaccaaa agtaaatgag tctgataata cttcagttaa
    13141 agaaactact gaagaacctc aaaatacaac ttctacacaa ccaactaaaa aaaataacga
    13201 tgcgacagca aataaagata atttagcggc tcaaaatatt tcaacgcagg ctaatgatgt
    13261 ttcagctact cctaaaacaa cgactattaa accaagaaca ttaaaccgta tggctgtgaa
    13321 cacagtagca gcaccacaac aaggtacaaa tgtaaatgat aaagttcatt tttcaaatat
    13381 tgatattgct attgataaag gacatgtcaa ttctacaaca ggtaaaactg aattttgggc
    13441 gacttctagt gatgttttaa aattaaaagc gaattacaca atcgatgatt ctgttaaaga
    13501 gggcgataca tttacattta aatatggaca gtatttccgt ccgggttctg taagattacc
    13561 ttcacaaact caaaatttat ataatgccca aggtaatatt attgcaaaag gtatttatga
    13621 tagtacaaca aacacaacaa catatacttt tacgaactat gtggatcaat atacaaatgt
    13681 tagtggtagc tttgaacaag ttgcatttgc gaaacgtgaa aatgcaacaa ctgataaaac
    13741 tgcttataaa atggaagtaa ctttaggtaa tgatgcgtat agcgaagaaa tcatcgtcga
    13801 ttatggtaat aaaaaagcac aaccgcttat ttcaagtaca aactatatca ataatgaaga
    13861 tttatcacga aatatgactg tttatgtaaa tcaacctaaa aatacataca caaaagaaac
    13921 atttgttagt actttaacag gttataaatt taatccagat gcaaaaaact ttaaaattta
    13981 tgaagtgaca gatcaaaatc aatttgttga tagctttaca cctgatactt caaaacttat
    14041 agatgttact gataaattca aaatcacata cagcaatgat aataagactg caacagttga
    14101 tttaatgaat ggtcaaacaa atagtaataa acaatatatt attcaacaag ttgcttatcc
    14161 agataataca tcaacagata atggaaaaat tgattataca ttagatacag acaaaactaa
    14221 atacagttgg tcgaacagtt attcaagtgt aaacggctca tcaactgcta atggcgacca
    14281 aaagaaatat aatctaggtg actatgtatg ggaagataca aataaagatg gtaaacaaga
    14341 tgccaatgaa aaagggatta aaggtgttta tgtcattctt aaagatagta acggtaaaga
    14401 attagatcgt actactactg atgaaaatgg taaatatcag tttacaggat tgggaaatgg
    14461 gacttacagt gtagaatttt caacgctagc agggtacaca ccaactactg taaatgcagg
    14521 aactgatgat gctgtagatt ctgatggact aactacaaca ggtgtcatta aagacgctga
    14581 taacatgaca ttagacagtg gtttctataa aacgccaaaa tacagtttag gtgattatgt
    14641 ttggtacgac agtaataaag atggcaaaca agattcaact gaaaaaggaa ttaaaggtgt
    14701 taaagttact ttgcaaaatg aaaaaggcga agtcattggt acaactgaaa cagatgaaaa
    14761 tggtaaatac cgctttgata atttagatag cggtaaatac aaagttattt ttgaaaagcc
    14821 tgctggctta aaacaaacag gtacaaatac aactgaagat gataaagatg cagatggtgg
    14881 cgaagttgac gtaacaatta cggatcatga tgatttcaca cttgataacg gatacttcga
    14941 agaa
//
LOCUS       DAMZPP010000070        14921 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMZPP010000070 DAMZPP010000000
VERSION     DAMZPP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14921
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.070"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(638..2239)
                     /locus_tag="POH73_001592"
     CDS             complement(638..2239)
                     /locus_tag="POH73_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6887541.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNVSFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSFKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            2386..3288
                     /gene="cvfB"
                     /locus_tag="POH73_001593"
     CDS             2386..3288
                     /gene="cvfB"
                     /locus_tag="POH73_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HDI6887542.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDHLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKH
                     KIINIETGKITLTKKGWSRIESKE"
     gene            3599..4582
                     /locus_tag="POH73_001594"
     CDS             3599..4582
                     /locus_tag="POH73_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDI6887543.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEGGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            4773..5699
                     /gene="pstC"
                     /locus_tag="POH73_001595"
     CDS             4773..5699
                     /gene="pstC"
                     /locus_tag="POH73_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDI6887544.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            5701..6618
                     /gene="pstA"
                     /locus_tag="POH73_001596"
     CDS             5701..6618
                     /gene="pstA"
                     /locus_tag="POH73_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDI6887545.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKYSKKF"
     gene            6665..7516
                     /locus_tag="POH73_001597"
     CDS             6665..7516
                     /locus_tag="POH73_001597"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDI6887546.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPTVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            7523..8164
                     /gene="phoU"
                     /locus_tag="POH73_001598"
     CDS             7523..8164
                     /gene="phoU"
                     /locus_tag="POH73_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDI6887547.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYNINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(8304..10118)
                     /gene="pepF"
                     /locus_tag="POH73_001599"
     CDS             complement(8304..10118)
                     /gene="pepF"
                     /locus_tag="POH73_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDI6887548.1"
                     /translation="MSQGLPLREDVPVSETWDLTDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEKSCPYQHYIKQLIKQKPFQLSASVEQLLAILS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTLNEMLMANYLFNKSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYRKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
     gene            10323..10667
                     /locus_tag="POH73_001600"
     CDS             10323..10667
                     /locus_tag="POH73_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887549.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYG
                     YEGAELRRKDKK"
     gene            10615..10800
                     /locus_tag="POH73_001601"
     CDS             10615..10800
                     /locus_tag="POH73_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887550.1"
                     /translation="MKNTAMRALNFAVKIRNNIRQLYAQNHQLLSYTAVTIISKVVEY
                     SAIVVFVLTFLYFTWLT"
     gene            10971..11957
                     /locus_tag="POH73_001602"
     CDS             10971..11957
                     /locus_tag="POH73_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6887551.1"
                     /translation="MFIIKSILYRLIQMIVVLFVISTLAFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKLSDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVLTLSLGMCAYIIRLVRSNLLILLKSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKNKTNTPLISESSDRHA"
     gene            11950..12780
                     /locus_tag="POH73_001603"
     CDS             11950..12780
                     /locus_tag="POH73_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6887552.1"
                     /translation="MHKIFSKNNLIFFVFGAFIFVMIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITVIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPIRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYNESH
                     "
     gene            12767..13540
                     /locus_tag="POH73_001604"
     CDS             12767..13540
                     /locus_tag="POH73_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6887553.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIYNQQINALIGESGAGKS
                     LIAKALLEYLPFDLTCTYDSYQFDGENVSRLSQYYGHTIGYIYQNYAESFNDHTKLGK
                     QLTAIYRKHYKSCKEEALSKIDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LKPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            13533..14234
                     /locus_tag="POH73_001605"
     CDS             13533..14234
                     /locus_tag="POH73_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6887554.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLIQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFTIEELFNV
                     DRHPYTKELVQAFSY"
     gene            14357..14815
                     /locus_tag="POH73_001606"
     CDS             14357..14815
                     /locus_tag="POH73_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDI6887555.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHETEVKGSGN
                     KVYRCYIDMEHPRKSKCNCPLADGRRVICKHMIALLFTASPEAANKHIIMLNEVEEDY
                     HLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEDEFY"
BASE COUNT         5140 a         2288 c         2524 g         4969 t
ORIGIN      
        1 taaactttac tcatgattga tgcgcctcat caaaatatta ttaaattggt atgtgttcat
       61 tatacatatg acaaatatga atgtaaaccg ataatttaga ttttttggaa taacctgaaa
      121 attcaagtta tagcgttgct tatattataa aaggtggtga gaatgaaact tttttgaaaa
      181 ataaaatccg aaatacttat agcgtactca atatgcacat taaataaatg tactctttta
      241 atgcgttgat aactgtattt gtaatttaga gaaggggtgt tcactgtgct gatgtgctaa
      301 aaaataaaaa agacacctcg atgctataaa tattagcatc gagacgtcca gttaatgtct
      361 attaaattga atatagtctc ggacatgaat caatgcccta ggccctgcaa tgttatattg
      421 acagtagttg actgaatgaa aatgactttg tacctagctt ttttcaatac ttgtcagcgt
      481 ctcacataga gaaattggat tcctaatttc tacaaacaat acgagttggg gaataagtcc
      541 caatatagaa ggtgacagta atgtcaactt acaaaaatgt gcaagttggc cgggcctcaa
      601 cacagagatt ttcgaaaaga aattctacaa attatattta ttttaaaacg ccgatttctt
      661 gcaagtattc ttcatatgga atttcttttg aaacgccgcc ttgtttattt aaatcgataa
      721 cacggtttgc aatcgtgttg ataaattcaa agtcataaga agtaaagatg atagaacctt
      781 taaatgattt aaggccatca ttgacagcag taatactttc taagtctaag tggttagtag
      841 gttcgtcaag taaaagtaca ttcgcacttg ataacatcat tttacttagc atacaacgta
      901 ctttttctcc acctgaaagc acactagctt ttttcttaac ttcttcacca ctaaataaca
      961 tacgacctaa gaaaccacgt aaaaatgttt ctgtttgttc atcttcagga gcatattgtc
     1021 ttaaccaatc aacgagattc atatttacac cctcaaagaa ctctgagtta tctttaggga
     1081 agtaacttaa cgatgtagta acaccccatt taaatgaacc ttcgtctggt tccatttcgc
     1141 cagctaatat tttaagtaat gttgtttttg caatttcact atctccaatt aaaatcgctt
     1201 tatcatttgg attcattgtg aaggatacat tatctaatac tttttcgccg tcaattgttt
     1261 tagaaagatt ttgaacgatt aataagtcgt taccaatctc acgctcaggc gtgaatttaa
     1321 cgaaaggata tcttcttgat gatggttgaa tatcatctaa ttcaattttc tcaagttgtt
     1381 ttttacgact tgttgcttgt ttagatttag aagcgttagc tgagaaacga gcaataaagt
     1441 cctgtaactc tttcattttt tcttcttttt tcttgttttg ttcttgagcc atcttttgag
     1501 ctaactgact agattgatac caaaaatcat agttaccaac ataaacttta attttaccaa
     1561 agtctaaatc agcgatatga gtacatacat tatttaagaa atgacggtca tgtgatacta
     1621 cgataacagt attatcaaag ttaattaaga aatcttctaa ccaactgatt gctggaatat
     1681 cgagaccgtt agtaggctca tccagtaata gtacgtctgg ttcaccgaat aaactttgcg
     1741 ctaataatac tttaattttt tggttgtttt ctaattcagc cattttttta tcgtgtaaag
     1801 ttggatcgat acctaaaccg gataaaaggt tagctgcatc agcttcagca ttccaaccat
     1861 tcatttctgc aaattcacct tcaagttcag cagcacggat accatcttca tcactgaaat
     1921 ctggcttcat atagatttca tctttttctt tcataacctc ataaagacgt tcgtgacctt
     1981 taattacaac atcaagcacg cgttcatctt cataagcata gtggtcctgt tttaaaacag
     2041 ctagacgttc atttttacct aatgaaacat gtcctgtttg agaatctaat tcaccagata
     2101 atatttttaa gaatgttgat ttacctgcac cattcgcacc aattaatcca taacaattac
     2161 cttctgtaaa tttaatattt acatcttcaa atagtttacg atctccaaaa cgtaaactca
     2221 catcagttac ttgtaacatg cattttctcc tttttttcat tcgatattct aacggaagaa
     2281 ttatatcata ttatcgtcac agtttcgacc tcatataagt tgtaatgata gaatgactca
     2341 cacatgttat aataataaag aatacaagaa tcgaaggaga ataacatggc attagacaaa
     2401 gatatagtag gttctataga attccttgaa gtagtagggt tacaaggctc aacttacctt
     2461 ttaaaaggac caaacggtga aaacgtaaag ttaaaccaat cagaaatgaa cgatgatgat
     2521 gaattagaag taggtgaaga atatagtttc ttcatttatc caaaccgttc aggtgaatta
     2581 tttgcaactc aaaatatgcc tgatattacg aaagataaat atgattttgc taaagtactt
     2641 aaaacggatc gcgatggggc acgtatagat gttggattac cccgtgaagt gttagtacca
     2701 tgggaagatt taccaaaagt gaaatcacta tggccacaac ctggagatca tttgctagtt
     2761 acattacgaa ttgaccgtga gaatcatatg tatggacgtt tagcgagtga atctgttgta
     2821 gaaaatatgt ttacacctgt acacgacgat aatttaaaaa acgaagtcat tgaagccaaa
     2881 ccataccgcg tattacgaat tggtagcttc ttattaagcg aatcaggtta caaaattttt
     2941 gtacatgaat cagaacgtaa agctgaaccg agactaggtg aatctgttca agttagaatt
     3001 atcggtcata atgataaagg tgagttaaat ggttcatttt taccacttgc acatgaacgt
     3061 ttagacgatg acggtcaagt catctttgat ttactagttg aatatgatgg tgaattacca
     3121 ttctgggaca aatcaagccc tgaagcgatt aaagaagtat tcaatatgag taaaggttca
     3181 ttcaaacgtg caatcggtca cttatataaa cataagatca ttaatataga aactggtaaa
     3241 atcactttaa ctaaaaaagg ttggagtcga attgagtcaa aagaataatc atttttacac
     3301 gtatcgtagg atgcgtgttt ttttattcaa tattaaatcg gacagatgaa gtagattttt
     3361 aatttcatct gtccattttt tgtgttcata tttaaatttg gaaaatttat caggaaacat
     3421 ttcaatttta ctgatgaagc aatatttttc agattaacaa aaaataatat ttacattttc
     3481 ttaacaaaca tttatgtaac atttacagtt tctaaagatg aggttaataa ttcgaggtta
     3541 agataaagat gtaatcaata caaatactat ttgttgttca tacagggagg atatttcaat
     3601 gaaaaaatgg caatttgttg gtactacagc tttaggtgca acactattat taggtgcttg
     3661 tggtggcggt aatggtggca gtggtaatag tgatttaaaa ggggaagcta aaggggatgg
     3721 ctcatcaaca gtagcaccaa ttgtggagaa attaaatgaa aaatgggctc aagatcactc
     3781 ggatgctaaa atctcagcag gacaagctgg tacaggtgct ggtttccaaa aattcattgc
     3841 aggagatatc gacttcgctg atgcttctag accaattaaa gatgaagaga agcaaaaatt
     3901 acaagataag aatatcaaat acaaagaatt caaaattgcg caagatggtg taacggttgc
     3961 tgtaaataaa gaaaatgatt ttgtagatga attagacaaa cagcaattaa aagcaattta
     4021 ttctggaaaa gctaaaacat ggaaagatgt taatagtaaa tggccagata aaaaaataaa
     4081 tgctgtatca ccaaactcaa gtcatggtac ttatgacttc tttgaaaatg aagtaatgaa
     4141 taaagaagat attaaagcag aaaaaaatgc tgatacaaat gctatcgttt cttctgtaac
     4201 gaaaaacaaa gagggaatcg gatactttgg atataacttc tacgtacaaa ataaagataa
     4261 attaaaagaa gttaaaatca aagatgaaaa tggtaaagca acagagccta cgaaaaaaac
     4321 aattcaagat aactcttatg cattaagtag accattattc atttatgtaa atgaaaaagc
     4381 attgaaagat aataaagtaa tgtcagaatt tatcaaattc gtcttagaag ataaaggtaa
     4441 agcagctgaa gaaggtggat atgtagcagc accagagaaa acatacaaat cacaattaga
     4501 tgatttaaaa gcatttattg ataaaaatca aaaatcagac gacaagaaat ctgatgataa
     4561 aaagtctgaa gacaagaaat aataagacgc aatttcaaat gtgtcttgaa acatgatttt
     4621 gatggtgaat cattatttag agtgcaaagc ttgatttatc gagacgctga ttttgacatt
     4681 cagttagtct acaagcttat caacttaaaa tagtggttca tcattatttt acaaatctaa
     4741 ttattttggg agtaatagaa agaggtttga ttatgacttc atctactaat gttaaagctt
     4801 taattgaaaa aaataataat aaaaaaggga agcataatga caaaattata ccagttattt
     4861 tagctgcaat ttcagcgatt tccattttaa caacactagg tatattaatc acattgcttt
     4921 tagaaaccat cacttttttc accagaattc caataactga atttctattt tctactactt
     4981 ggaatcctac cggctcagac cctaagtttg gtatctgggc attgataata gggactttaa
     5041 aaatcacagt tattgcgact atatttgcag ttccagtcgg gcttggtgca gcaatttatt
     5101 taagtgaata tgcaagtgac cgtgcacgac gcataataaa accgatatta gaaattttag
     5161 caggaatacc aacaattgtg tttggtttct ttgcattaac ctttgttaca ccagtattaa
     5221 gatctttcat acctggtctt ggagagttta atgctataag tcccggctta gttgtaggta
     5281 ttatgattgt ccctctcatc acaagtttga gtgaggatgc aatggcatct gtaccaaata
     5341 aaattcgaga aggtgcctat ggacttggag caactaaatt agaagtagca actaaggtcg
     5401 tacttcctgc agcaacatca ggtattgtag cttcaatagt tctcgcgatt tcaagagcaa
     5461 ttggagaaac gatgattgta tcattagcgg caggtagttc accaacagct tcattaagtt
     5521 taacaagttc aattcaaaca atgactggat atattgttga gatagcgaca ggtgatgcaa
     5581 catttggatc aaatatttat tacagtattt atgctgtagg attcacatta tttatcttta
     5641 ccttaatcat gaatttactt tctcaatgga tttctaagcg ttttagggag gagtattaat
     5701 atggaaacga cagataataa tagacaatca ctcgtcgatc aacaacttgt ccaaaaacat
     5761 ttatcatcca gaacggttaa aaataaagtg ttcaaactca tatttttagc atgtacatta
     5821 ttaggacttg tcgtacttat tgcgttgtta actcaaacat tgattaaagg ggtaagtcat
     5881 ttaaatttac agtttttcac taatttttct tcttcaacac catctatggc tggcgttaaa
     5941 ggcgcattaa tcggttcact ttggttaatg ttaagtatca ttccattatc aatcatccta
     6001 ggaataggta cagctatata cttagaagaa tatgcgaaaa acaacaaatt tactcagttt
     6061 gttaaaatca gtatttccaa tttagctggt gtaccatcag ttgtatttgg gttattaggt
     6121 tacactttgt tcgttggtgg tgccgggatt gaagccttga aaatgggtaa cagtatattg
     6181 gcagcagcgc taacaatgac cttactgata ttaccaatta ttattgtttc aagtcaggaa
     6241 gcaattagag ctgtacctaa ctcagtacgc gaagcttctt acggcttagg tgctaataaa
     6301 tggcaaacga taagacgtgt tgtcttacca gcagcgttac ctggtatttt aactggattc
     6361 attttgtctc tttcaagagc actgggagaa acagcgccac ttgtgcttat cggtatacca
     6421 actatattat tggcaacacc tagaagtata ttggatcaat tttcagcatt acctatccaa
     6481 atatttactt gggcgaaaat gccacaagaa gaattccaga atgttgcatc ggcaggcatt
     6541 atcgttttac tagttatctt aatcttaatg aatggcgttg cgattatttt acgtaacaaa
     6601 tatagtaaaa aattctaatt taaacaatca atctaattta tctattaaaa agggagtatt
     6661 aaatatggcg caaacacttg cacaaactaa acaaatatct caaagtcata cgtttgatgt
     6721 ctcacaaagt catcataaaa caccagatga tacaaactca cattctgtta tatattcaac
     6781 acaaaattta gacttatggt atggcgaaaa tcatgcattg caaaatatta atttagatat
     6841 ttatgaaaac caaattactg ccattatagg tccatctggt tgtggcaaat caacatatat
     6901 taaaactttg aatcgaatgg ttgagttagt acccacagtc aaaacagctg gtaaaatact
     6961 atatcgcgat caagacattt ttgatcaaaa atattctaaa gaacaattac gtacaaatgt
     7021 gggcatggtc ttccaacaac ctaatccatt tccaaaatca atatacgata atattactta
     7081 cgggccaaag attcacggta ttaaaaataa aaaagttctt gatgaaatcg ttgagaaatc
     7141 attacgtggc gctgcaattt gggatgaatt aaaggatagg ttgcacacaa atgcatatag
     7201 tttatccggt gggcaacaac agcgtgtttg tatcgcgcgt tgtttagcaa ttgaacctga
     7261 agtcatttta atggatgaac ccacatcagc attagatcca atctcaacat taagagtaga
     7321 agagttggtt caagaactaa aagaaaagta cacaattatt atggttacac ataatatgca
     7381 acaagcagct cgtgtatcag ataaaactgc atttttctta aatggttatg tcaatgaata
     7441 tgatgacact gataaaattt tctctaaccc atcaaacaag aaaacagaag attatatttc
     7501 aggaaggttt ggttgatata taatggcaat aattagacaa cgatatcagg agcaacttga
     7561 tgatttaata aaagaattac gtcggttagg tgcaaatgtc tatgtgagta ttgaaaatgg
     7621 tataaaatca ttaagtattg acgatagagg ctttgcacga caaacagtta aaaacgataa
     7681 acatatcaat caattaaatt ataatattaa tgagcgagtt atcatgttaa ttacaaagca
     7741 acagcccatt gcgagtgatt tgcgtatgat gatttcttca ttaaaaatcg cctccgattt
     7801 agaaagaata ggagataatg catcgagtat tgccaatatt cgattgcgta cgaagattac
     7861 agatgattat gtgttaaccc gtttaaagac aatgggtaaa ctagctatgt taatgttaaa
     7921 ggatttagat caagcattta aaaagaaaga taccgtatta ataagagaaa taattgagcg
     7981 tgatgaagat atcgatgact tatatagtca tattattaac gcaacgtatc ttattgataa
     8041 cgatccattt gtcgctgcac aagcccattt agcagcaaga catctagaac gtattggtga
     8101 tcatattatt aacatcgctg aaagtgttta tttttattta acaggtacac attacgaaca
     8161 ataacttaaa gttattacta taaaatccct tacgataaat atatatttct attattcata
     8221 aaccctcaaa aaaaccaaga ttctcacaat tagtaatgtg aaaatcttgg tttatattgt
     8281 tctactataa gttgtctcgc atcctagtta tttgcttgct caatttcatc tgttaatttt
     8341 tcaacttcat ccactaaatc agaaatatat tgaattgtag atttaagtgg ctgttctgta
     8401 gtaatgtcta cacccgcaat gtttgcaagt tcgacaggtg atacactacc accttttttc
     8461 aatgtttcta accaagcatc aacagctggt tggccttcat ttttaatctt ttgagaaacg
     8521 acagtcccga ttgttaagcc agcagaatac gtatacgaat ataatcccat atagtaatga
     8581 ggttgacgca tccatgttaa ttcagcaccc tcagtcatgt ctactgcatc tccgaaaaat
     8641 tgtttataaa catttagcat tatttcattt aatgtcggcg catttaaaga ttcaccttga
     8701 tctactttgc gatacacttc acgttgataa gcagcttcta ataagtgggt aaccatatta
     8761 tgataatatg ttctagataa aattgagcca ataacccaac gcttaaatct tggattatca
     8821 cttttgttaa ataaataatt ggccatcaac atttcattca atgtagaagg ggcttcaaca
     8881 aagtacattg atgcttctga ttcaagatat ggttgatgtt tttgagctaa tgtaaaatga
     8941 cctgcatgac ctaattcatg tgctaagaca aatgcttcag ccattttacc agtccaagaa
     9001 ataaacacat atgaatgtgt aaagtatgga cttgcacaaa atgcgcctgt atctttacct
     9061 ttattttgtg caaaatcaat ccatcgctga tcgtatgctt cacgtaacat gtttgtatag
     9121 tcatcaccta aaacacttaa cgcaccgaaa atataatttt ttgagtcttc aattgaaatc
     9181 tctggttcat aatcagggtc tacagaaatt tttaagtctt caaaacgcat gttatctaat
     9241 ccgtgaatac gttgtaaaag tttagcatat ttctgcataa ctggtgccaa gtcacgcata
     9301 atcatatcga tttgacggtc aaacatatca cgcgttactt cttgactatg taataaataa
     9361 tcgatgaccg attcaaaccc acgtaaatca gcttcaattt tttcttgttg tacttgcata
     9421 ttatatgtag ccgcggtagt atgctgatat tttcgaatcc catcgctaaa cgatttgaaa
     9481 cttttacgtc taaactcagg atctttatta tcttcataat cattttcaaa cgtagcatag
     9541 tcgacagggt acgttgtacc atcatgttca aatgaatcga atgtaatatc tagcattttt
     9601 gtcgtgccgt ataaatcgta aggactgttt agcgtaggtg ataaaattgc taataattgt
     9661 tctaccgacg cagataattg gaatggcttt tgttttatta actgtttaat atagtgttga
     9721 tatggacatg atttttctaa ttgttgaagt atttcttctg gtaattcaag tatttctgac
     9781 tctacaaatg ataattggct aacaatttta ccgtatgtag tagataattt agcgctcaat
     9841 acttgtgcct caatattact agtatctaca cttaaacgta gttctgcata attacttaag
     9901 cgatctaagg caattaaaat attttctaat tcagctaaag cagtattaat ttgttcgatt
     9961 gaatttaatg ttgttgcata tgtatgatga aattgatttg cttgttgtac tagagcgtca
    10021 atactttcat aatattgttg atcatcttta aataagtctg ttaaatccca tgtttctgaa
    10081 acaggaacat cttctcttaa aggtaaacct tgagacatat aatcactcct ttggtataat
    10141 acattcatta tacataattt tgtctcctaa acgataatgt tacgattccc atgtcgcaag
    10201 gaacaatgat ttttctaata gtagatagaa tattgcttga aactgactgc tattgtttaa
    10261 gtttttaaaa atttataata aaatgtatac aaggaaagta atgaaataaa aggagaatga
    10321 atatgacatt attcgatatg cctaattatt tatggatcac aactttaata atgattttat
    10381 taacaatatt ctgttgctta gttttaaata aatggtttgt atcagcagta attacatttg
    10441 ttatattagg tgtgcttgca ttttttattc caaattttca agatataaaa tatcaaccat
    10501 tattaggata cgcggcgttt ttagcgatca tgagtttatt aattagtttt ctattatggt
    10561 attttacaag aaattggcgt aaagaaagaa aagcgcgtaa attagaaaag caaattgaaa
    10621 aatacggcta tgagggcgct gaacttcgcc gtaaagataa gaaataatat tcgacaacta
    10681 tatgcacaaa atcatcaact tttaagttac actgcagtaa caattatttc gaaagtggtg
    10741 gaatacagtg ctatagttgt ttttgttttg acttttttat attttacatg gttgacttga
    10801 ttctaaatat tatattttat aatgattttg ttaaaaaatt aaatatactt caatttcttt
    10861 ttggataata ctataatgaa cgttagttct taatatgtaa gttgcatata catgcgccat
    10921 tctcatgtaa atagaaagtg ttagtaattt acacaaagag ggggactatt atgttcataa
    10981 taaagtctat tttgtataga ctgatacaaa tgatagttgt tttatttgtt atttctacac
    11041 ttgcattcat tttaatgaaa ttatcaccag gcaatcctgt tgataaaata ttacatcttg
    11101 atgtagcaca agtgtcgacg gaacaaataa atgcaaccaa agataaattg ggattaaatg
    11161 attctttatt ggtacagtgg tggcattgga tgaatcattt attacatttt aatttaggaa
    11221 aaagttttga atcaaaagaa ccggtaacac agatattatt caactatgca cccataacac
    11281 tactcatatc tttttcaaca ttagtagtgt cattatgcat atctataccg ctcggcatta
    11341 tagcagccaa acgatttcat aagttatctg acaaagtgat tagagtgatc tcaactctat
    11401 caattagttt accagcgttc tttatcggta ttattttatt atttattgtc acaaatttaa
    11461 tgaatataga tagcgttata ctaagtcagt ttatattacc tgtactcacg ctatctttag
    11521 gtatgtgtgc atacatcatt cgcttagtgc gttctaattt attgatatta ttgaaaagta
    11581 atatcgtaca agcatcaaga ttacgcggta tgaatgaacg ctatatttta attcatgatt
    11641 tactaaaacc aacaattttg ccgattatcc cattattagg gatttcactt ggtagtctaa
    11701 taggtggtac tgtagtgatt gaaaatttat ttgatatacc tggtattggt tatctattaa
    11761 tggatagtat taaatctcga gattatcctg ttattcaagg atgcgtgtta tttattggct
    11821 tcttcgttgt tattatcaat acgattgctg atttattaac gttattactt gatccgaagc
    11881 agcgtttaca attaggaaat ccgaaaaaca aaaccaatac accattgata tcagaaagta
    11941 gtgaccgtca tgcataaaat attttcaaag aataacctta tcttttttgt attcggcgca
    12001 tttatatttg tgatgattgt actacaattc tttgtcagta gtgaaaatgc aaccaaagtc
    12061 aatttatcac aaacttttga acctattagt tggttgcatt tattaggaac tgatgattat
    12121 gggagagatt tatttacccg aattattatc ggtgcacgtt caacattgtt tgttactgtt
    12181 ttaacattaa tagctatcgt tgtcataggt gttacactag gtctatttgc cggatataaa
    12241 aaggggtgga ttgaacgatt agtgttaagg tttattgatg ttggtctaag tattccagaa
    12301 tttatcatca tgattgcttt agcaagcttt ttccaaccat cgttatggaa tttagttatt
    12361 tcaattacag taataaaatg gatgaattac acaaggttga ctagaagtat agttaatagc
    12421 gaaatgaata agccttatat aaaaatggca caattatttc atgtacctat aagaacaata
    12481 ttaatacgtc atttaacacc taaaattata ccggctatta tcgttttgat ggtcgttgat
    12541 ttcggtaaaa tcattctata tataagttca ctatcattta ttgggttagg tgcacaaccg
    12601 ccaacaccag agtggggcgc tatgttgcaa caaggtcgtg attttatttc gtctcatcca
    12661 attatgttga ttgcacctgc ttcagtcatt gctataacta ttttaatttt taatttaacc
    12721 ggtgatgcgc taagagatag attgctgaaa caacgaggtg aatataatga gtctcattga
    12781 tatacaaaat ttaacaataa agaatactag tgagaaatca cttattaaag ggattgattt
    12841 gaaaatttat aatcaacaga ttaatgccct gattggagag agcggcgctg gaaaaagttt
    12901 gattgctaaa gccttacttg aatatttacc atttgattta acctgcacgt atgattcgta
    12961 ccaatttgat ggggaaaatg ttagtagatt gagtcaatat tatggtcata caattggcta
    13021 tatttatcaa aattatgcag aaagttttaa cgaccatact aaattaggta aacagttaac
    13081 tgcgatttat cgtaagcatt ataaaagttg taaagaagag gctttgtcca aaattgacaa
    13141 ggctttgtcg tgggttaatt tacaaagcaa agatatatta aataaatata gtttccaact
    13201 ttctgggggc caacttgaac gcgtatacat agcaagcgtt ctcatgttga aacctaaatt
    13261 aatcattgca gacgaaccag ttgcatcatt ggatgctttg aacggtaatc aagtgatgga
    13321 tttattacag catattgtat tagaacatgg tcaaacatta tttattatca cacataactt
    13381 aagtcatgta ttgaaatatt gtcagtacat ttatgtttta aaagaaggtc aaatcattga
    13441 acgaggtaat attaatcatt tcaagtatga gcatttgcat ccgtatactg aacgtctaat
    13501 taaatataga acacaattaa agagggatta ctatgattga gttaaaacat gtgacttttg
    13561 gttataataa aaagcagatg gtgctacaag atatcaatat tactatacct gatggagaaa
    13621 atgttggtat tttaggcgaa agtggctgtg gtaaaagtac gctcgcttca ttggttcttg
    13681 gcttatttaa acctgttaaa ggagaaattt acttaagtga caatgctgtg ttaccgattt
    13741 tccagcaccc tttaactagc tttaaccctg attggacgat tgagacctca ttaaaagaag
    13801 cgttatatta ttacagaggt ctaactgata atactgctca ggaccaatta ttaatacaac
    13861 atttatctac ttttgagttg aacgcgcaat tattgactaa attaccaagc gaagtgagtg
    13921 gcggacaatt acaaagattt aatgtcatgc gttcgttatt agcacagcct cgcgttttaa
    13981 tatgtgatga gataacttca aatttagatg ttatagctga acaaaatgta ataaatatat
    14041 taaaagcgca aacgattacg aacttaaatc attttatcgt tatttctcat gatttatccg
    14101 tgttacaacg cttagtcaat agaattatcg ttcttaagga tggcatgata gtcgatgatt
    14161 ttacaataga ggaattattt aatgttgata gacaccctta tacaaaagaa ttagtgcaag
    14221 cattttcata ttagttattt aagaatgcga taattctaga cttgttataa aatatagata
    14281 aatcaagtat attaatctag acacatatcg attttatttt ctatatctaa aaataataat
    14341 aaaaaggagt atcatcatgg gattacttga tattgcaagt attcgttcta tagaaagagg
    14401 ctttaattat tatcaaagtg aatgcgtaat taacttaaaa tcattttcag aaacgcagca
    14461 tgagactgaa gtaaagggca gtggtaacaa agtatatcgt tgttatattg atatggaaca
    14521 tcctagaaaa tccaaatgta attgtcctct tgctgatgga agacgagtga tatgtaaaca
    14581 tatgattgca ttactcttta cagctagtcc agaagcagca aataaacata taatcatgtt
    14641 aaacgaagtt gaagaagact atcacttacg cagaaatatg tggattgatt ctcttaaaga
    14701 aatgattaat gatatgagtg aagaagaact ccgcgacgca tatttaaaca tgttaattga
    14761 acatggagaa atggcagaat tatttggatt agatgaagag gacgaatttt attaaaatag
    14821 cccctatcca ttgataatga ttatcatttg ataggggtgt ttttatttat atgattttaa
    14881 gactttgcaa atagctagtg cattattatc gatgtgtttg a
//
LOCUS       DAMZPP010000071        14879 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMZPP010000071 DAMZPP010000000
VERSION     DAMZPP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14879
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.071"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..168
                     /gene="truB"
                     /locus_tag="POH73_001607"
     CDS             <1..168
                     /gene="truB"
                     /locus_tag="POH73_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDI6887556.1"
                     /translation="IKKRILNGQKFNKNEFDNKIKDQIVFIDDDSEKVLAIYMVHPTK
                     ESEIKPKKVFN"
     gene            183..1154
                     /gene="ribF"
                     /locus_tag="POH73_001608"
     CDS             183..1154
                     /gene="ribF"
                     /locus_tag="POH73_001608"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDI6887557.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVNKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            1269..1538
                     /gene="rpsO"
                     /locus_tag="POH73_001609"
     CDS             1269..1538
                     /gene="rpsO"
                     /locus_tag="POH73_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDI6887558.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
     gene            1907..4003
                     /gene="pnp"
                     /locus_tag="POH73_001610"
     CDS             1907..4003
                     /gene="pnp"
                     /locus_tag="POH73_001610"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDI6887559.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPTERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            4239..5912
                     /locus_tag="POH73_001611"
     CDS             4239..5912
                     /locus_tag="POH73_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDI6887560.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKEKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            6169..8538
                     /locus_tag="POH73_001612"
     CDS             6169..8538
                     /locus_tag="POH73_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDI6887561.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHRDVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLIEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            8543..9256
                     /locus_tag="POH73_001613"
     CDS             8543..9256
                     /locus_tag="POH73_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6887562.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLLPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            9287..10552
                     /locus_tag="POH73_001614"
     CDS             9287..10552
                     /locus_tag="POH73_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDI6887563.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            10552..11838
                     /locus_tag="POH73_001615"
     CDS             10552..11838
                     /locus_tag="POH73_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDI6887564.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEENAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFNTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            11838..12542
                     /locus_tag="POH73_001616"
     CDS             11838..12542
                     /locus_tag="POH73_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI6887565.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTNKFN
                     DDKVHFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSDNGRIIVISSIWGETGASMETIYSTMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            12647..13474
                     /locus_tag="POH73_001617"
     CDS             12647..13474
                     /locus_tag="POH73_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HDI6887566.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            13493..13885
                     /locus_tag="POH73_001618"
     CDS             13493..13885
                     /locus_tag="POH73_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6887567.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            13919..14497
                     /gene="pgsA"
                     /locus_tag="POH73_001619"
     CDS             13919..14497
                     /gene="pgsA"
                     /locus_tag="POH73_001619"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDI6887568.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
     gene            14722..>14879
                     /locus_tag="POH73_001620"
     CDS             14722..>14879
                     /locus_tag="POH73_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDI6887569.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSV"
BASE COUNT         5451 a         1984 c         2828 g         4616 t
ORIGIN      
        1 ataaaaaaac gtattttaaa tgggcagaaa tttaataaaa atgaatttga taacaaaatt
       61 aaagaccaaa ttgtatttat tgatgatgat tcagaaaaag tattagcaat ttatatggta
      121 caccctacaa aagaatcaga aattaaacct aaaaaagtct ttaattaaag gagatagaat
      181 ttatgaaagt catagaagtg acacatccta tacaatctaa acagtatatt acagaggatg
      241 ttgcaatggc attcggattt ttcgatggca tgcataaagg tcatgacaaa gtctttgata
      301 tattaaacga aatagctgag gcacgcagtt taaaaaaagc ggtgatgaca tttgacccgc
      361 atccgtctgt cgtgttgaat cctaaaagaa aacgaacaac ctatttaaca ccactttcag
      421 ataaaatcga aaaaattagc caacatgata ttgattattg tatagtggtt aatttttcat
      481 ctaggtttgc taatgtgagc gttgaagatt ttgttgaaaa ttatataatt aaaaataatg
      541 taaaagaagt cattgctggt tttgatttta cttttggtaa atttggaaaa ggtaatatga
      601 ctgttcttca agaatatgac gcgtttaata cgacaattgt gaataaacaa gaaattgaaa
      661 atgaaaaaat ttctacaact tctattcgtc aagatttaat caatggtgag ttgcaaaaag
      721 cgaatgatgc tttaggctat atatattcta ttaaaggcac tgtagtgcaa ggtgaaaaaa
      781 ggggaagaac tattggcttc ccaacagcta acattcaacc tagtgatgat tatttgttac
      841 ctcgtaaagg tgtttatgcc gttagtattg aaatcggcac tgaaaataaa ttatatcgag
      901 gggtagctaa cataggtgta aagccaacat ttcatgatcc taacaaagca gaagttgtca
      961 tcgaagtgaa tatctttgac tttgaggata atatttatgg tgaacgagtg acagtgaatt
     1021 ggcatcattt cttacgtcct gagattaaat ttgatggtat cgacccatta gttaaacaaa
     1081 tgaacgatga taaatcgcgt gctaaatatt tattagcagt tgattttggt gatgaagtag
     1141 cttataatat ttagagttgc gtatagttat ataaacaatc tataccacac ctttttctta
     1201 gtaggtcgaa tctccaacgc ctaactcgga ttaaggagta ttcaaacatt ttaaggagga
     1261 aattgattat ggcaatttca caagaacgta aaaacgaaat cattaaagaa taccgtgtac
     1321 acgaaactga tactggttca ccagaagtac aaatcgctgt acttactgca gaaatcaacg
     1381 cagtaaacga acacttacgt acacacaaaa aagaccacca ttcacgtcgt ggattattaa
     1441 aaatggtagg tcgtcgtaga catttattaa actacttacg tagtaaagat attcaacgtt
     1501 accgtgaatt aattaaatca cttggtatcc gtcgttaatc ttaatataac gtctttgagg
     1561 ttggggcata tttatgttcc aaccttaatt tatattaaaa agttttttac aaatattaac
     1621 atttattata tgttaagcta atattgagtg aataataagg ttacaatgag ataaagataa
     1681 tataagtaca cctagagtaa taatcaagat attaaaaata aagtatgttt tttaaaaaaa
     1741 tataacttat atttatactg ataagggtgg gacgataagt ctattttgta aataatagat
     1801 ggatatcccg ctctcttttt ttccaattca atattttata actaatatta aaatacgata
     1861 ataaatgata tgatataact attagattca agagaggaga tttataatgt ctcaagaaaa
     1921 gaaagttttt aaaactgaat gggcaggaag atctttaacg attgaaacag ggcaattagc
     1981 taaacaagca aatggcgctg tattggttcg ttatggagat acagtcgtgt tatcgacggc
     2041 aactgcatca aaagaacctc gtgatggaga tttcttccca ttaacagtga actatgaaga
     2101 aaaaatgtac gctgcgggta aaattcctgg tggatttaaa aagagagaag gacgtcctgg
     2161 tgacgatgca acattaactg cgcgattaat tgatagacca attagacctt tattccctaa
     2221 aggatataag catgatgttc aaattatgaa catggtatta agtgcagatc ctgattgttc
     2281 accacaaatg gctgcaatga ttggttcatc tatggcgctt agtgtgtcgg atattccatt
     2341 ccaagggcca atcgccggtg taaatgtggg ttatattgat ggtaaatata tcattaaccc
     2401 aacagtagaa gaaaaagaag tttctcgttt agaccttgaa gtagctggtc ataaagatgc
     2461 agtaaacatg gtagaagcag gcgctagtga gattactgaa caagaaatgt tagaggcgat
     2521 tttctttggt catgaagaga ttcaacgttt agttgatttc caacaacaaa tcgtcgacca
     2581 cattcaacct gttaaacaag aatttattcc aacagagcgt gatgaagcgc tagttgaacg
     2641 tgtaaaatct ttaaccgaag aaaaaggact taaagaaaca gttttaacat ttgataaaca
     2701 acaacgagat gaaaatcttg ataacttaaa agaagaaatc gtcaatgaat ttatcgatga
     2761 agaagatcca gagaatgaat tacttattaa agaagtttat gcaattttaa atgaattagt
     2821 gaaagaagaa gttcgacgtt taattgcaga tgaaaaaatt agaccagacg gccgtaaacc
     2881 tgatgaaatc cgtccattag attctgaagt tggtatttta cctagaacgc atggttcagg
     2941 tctatttaca cgtggtcaaa ctcaagcact ttcagtttta acattaggtg ctttaggtga
     3001 ttatcaatta attgatggtt taggacctga agaagaaaaa agattcatgc atcattataa
     3061 cttcccgaat ttttcagtag gtgaaactgg tccagtacgt gcgccaggtc gtcgtgaaat
     3121 tggacatggt gcgttaggtg aaagagcatt aaaatatatt attcctgata ctgctgattt
     3181 cccatataca attcgtattg taagtgaggt acttgaatca aacggttcat catctcaagc
     3241 gtcaatttgt ggatcaacat tagctttaat ggatgcgggc gtaccgatta aagcaccagt
     3301 tgctggtatt gctatgggcc ttgttacacg tgaagatagc tatacgattt taacagatat
     3361 ccaaggtatg gaagatgcat taggtgatat ggactttaaa gtcgctggta ctaaagaagg
     3421 tattacagca atccaaatgg atattaaaat tgacggttta acgcgtgaaa ttatcgaaga
     3481 ggctctagaa caagcgagac gtggtcgttt agaaataatg aatcatatgt tacaaacaat
     3541 tgatcaacca cgtactgaat taagtgctta cgcgccaaaa gttgtaacta tgacaattaa
     3601 accagataag attagagatg ttatcggacc tggtggtaaa aaaattaacg aaattattga
     3661 tgaaacaggc gttaaattag atatcgaaca agatggtact atctttattg gtgccgttga
     3721 tcaagctatg ataaatcgtg ctcgtgaaat cattgaggaa attacacgtg aagcggaagt
     3781 aggtcaaact tatcaagcca ctgttaaacg tattgaaaaa tacggtgcgt ttgtaggcct
     3841 attcccgggt aaagatgcgt tgcttcacat ttcacaaatt tcaaaaaata gaattgaaaa
     3901 agtggaagat gtattaaaaa ttggtgacac aattgaagtt aaaattactg aaattgataa
     3961 acaaggtcga gtaaatgcat cacacagagc attagaagaa taatatttaa agtcatatga
     4021 cgacaatgta tcgtcatgtg atttttttat gccatttttt acgaagtgac ccattttgaa
     4081 tttgttgtat tgaacatttt aaaacgcttt attattttgt gtacaactgt taattatcct
     4141 gtatgtatag tgattaatag tgtacatcaa gtatttttta acttataatg aatagtgagt
     4201 ttatatatgg acgggtaaca aatttaggag gtaagatttt gagtttaata aagaaaaaga
     4261 ataaagatat tcgcattata ccattaggcg gtgttggcga aattgctaaa aatatgtata
     4321 tcgttgaagt agacgatgaa atgtttatgt tagatgctgg acttatgttt ccagaagacg
     4381 aaatgctagg tattgatatt gttataccag acatttcata cgtacttgaa aataaagaaa
     4441 aattgaaggg tatattcctt acacacggac atgagcacgc gattggtgca gtgagttatg
     4501 ttttagaaca attagatgca ccagtatatg gatctaaatt gacaatagcg ttaattaaag
     4561 aaaatatgaa agcccgtaat attgataaaa aagttcgcta ctacacagtt aataatgatt
     4621 caattatgag attcaaaaac gtgaatatta gtttctttaa tacgacacac agtattcctg
     4681 atagtttagg tgtttgtatt cacacttcat atggtgccat tgtgtataca ggtgaattta
     4741 agtttgacca aagtttacat ggacattatg caccagatat taaacgtatg gcagagattg
     4801 gtgaagaagg cgtatttgtc ttaatcagtg attctactga ggcagagaaa cctggatata
     4861 atactccgga aaatgtgatt gaacatcata tgtatgatgc ttttgcaaaa gtgcgaggtc
     4921 gcttgattgt ttcatgttat gcttcgaact ttatacgtat tcagcaagtt ttaaatattg
     4981 ctagcaagct aaatcgtaaa gtgtcatttt taggaagatc acttgaaagt tcatttaata
     5041 ttgctcgtaa aatggggtat ttcgacattc ctaaagattt gctaattcct ataacagaag
     5101 ttgataatta tcctaaaaat gaagtgataa ttatagctac tggtatgcaa ggagaacctg
     5161 tagaagcctt aagtcaaatg gcgcaacata agcataaaat tatgaatatc gaagaaggcg
     5221 attctgtatt tttagcaatt acggcttctg ctaatatgga agttatcatt gcgaatacat
     5281 taaatgagct tgtacgtgct ggcgcacata ttattccaaa taacaagaag attcatgctt
     5341 caagtcatgg ttgcatggaa gaattaaaaa tgatgattaa tattatgaaa cctgaatact
     5401 ttattcctgt acaaggtgaa tttaaaatgc agatagcaca tgcgaagcta gcagctgaag
     5461 caggtgttgc accagaaaag attttccttg tggaaaaagg agatgtcatt aattacaacg
     5521 gtaaagatat gatattaaat gaaaaggtaa attcaggaaa tattttaata gatggtattg
     5581 gtattgggga tgtaggaaat atcgtgttga gagaccgtca tcttttagca gaagatggta
     5641 tctttattgc tgtagtaacg ttagatccta aaaatagacg tatagctgcg ggacctgaaa
     5701 ttcaatctcg tgggtttgta tatgtacgtg aaagtgaaga cttattacgt gaagcagaag
     5761 agaaagtacg tgaaatagta gaagctggtt tacaagaaaa acgcatagaa tggtctgaaa
     5821 ttaagcaaaa tatgcgtgat caaattagta aactattatt cgaaagtaca aaacgtcgtc
     5881 ctatgattat tccagtaatt tctgaaattt aatcaaaaag tcattaacat aaaagaggtc
     5941 agaacaagtc accgaaatat aatggttgtc atggacaatt tacttatatt ttatgatggt
     6001 caattgaaag gggtaacgat taatctgtta tcttaagtaa attgatacat agatgatatt
     6061 gttctaacct ctttcatcgt ctgtttggac tacatattct aaacatcaaa taggaaatta
     6121 tatagattaa cgtcgtttta actaaagcaa cataaggagg tgcgtcaatt ggcacaagca
     6181 aaaaagaaat cgacagctaa gaaaaaaaca acatcaaaaa aaagaacaaa ttcgaggaaa
     6241 aagaagaatg ataatccgat acgttatgtc atagctattt tagtagttgt attaatggtg
     6301 ttgggtgttt tccaattagg aataataggt cgtctaattg acagcttctt taattattta
     6361 tttgggtaca gtagatattt aacatatatt ttagtactct tagcaactgg ttttattaca
     6421 tactctaaac gtattcctaa aactagacga acggctggtt cgattgtatt gcaaattgca
     6481 ttgctatttg tatcacagtt agtttttcat tttaatagtg gtatcaaagc tgaaagagaa
     6541 cctgtacttt cttatgtgta tcagtcatac caacacagtc attttccaaa ttttggtggc
     6601 ggtgtattag gcttttattt attagagtta agcgtacctt taatttcatt atttggtgta
     6661 tgtattatta ctattttatt attatgctca agtgttattt tattaacaaa ccatcaacat
     6721 cgtgatgttg caaaagttgc actggaaaat ataaaagctt ggtttggttc atttaatgaa
     6781 aaaatgtcgg aaagaaacca agaaaaacaa ttgaagcgtg aagaaaaagc gagacttaaa
     6841 gaagaacaaa aggcacgtca aaatgaacag ccacaaataa aagatgtgag tgattttacg
     6901 gaagtgcctc aagaaagaga tattccaatt tatgggcata ctgaaaatga aagtaaaagc
     6961 cagagtcaac caagtcgaaa aaaacgagtg tttgatgcag agaatagttc gaataacatc
     7021 gtaaatcatc atcaagcaga tcagcaagaa caattaatag aacaaactca taacagtgtt
     7081 gaaagtgaaa acactattga agaagctggt gaagttacga atgtgtcgta tgttgttcca
     7141 ccgttaactt tacttaatca acctgcaaaa caaaaagcaa catctaaagc tgaagtgcaa
     7201 cgtaaaggac aagtactaga gaatacatta aaagattttg gggtaaatgc aaaagtgaca
     7261 caaattaaaa ttggtcctgc agtaactcaa tatgaaattc aaccagctca aggggttaaa
     7321 gtgagtaaaa ttgtaaactt gcataatgat attgcattag ctttagcagc aaaagatgtt
     7381 agaattgaag cgccaatacc tggtcgctct gcagtaggta ttgaagtgcc aaatgagaaa
     7441 atttcattag tttcactaaa agaagtttta gatgaaaaat tcccgtctaa taataaacta
     7501 gaagttggat taggaagaga tatatcaggt gatccaatta ctgttccact aaatgaaatg
     7561 ccacacttat tggtggcagg atcgacgggt agtggtaaat ctgtttgtat aaatggtatt
     7621 attacaagta ttttattaaa tgctaagccg catgaagtta aacttatgtt aatcgatccg
     7681 aaaatggttg aactaaatgt ttataacgga attccacatt tattaattcc ggttgttaca
     7741 aatcctcata aagctgctca agctttagaa aaaattgtag ctgagatgga aagacgttat
     7801 gatttattcc aacattcatc aactagaaat attaaaggtt ataacgaatt aatccgtaag
     7861 caaaatcaag aattagatga gaagcaacca gaattacctt atatcgttgt tattgtagat
     7921 gagcttgcag atttaatgat ggtagctggt aaagaagttg aaaatgcgat tcaacgtatc
     7981 acacaaatgg cacgtgcagc aggtatacat ttgattgtag cgacacaaag accttctgtg
     8041 gatgtaatta caggtatcat taaaaataac attccatcta gaattgcttt tgctgtgagt
     8101 tctcaaacag attcaagaac tattattggt actggcggcg cagaaaagtt acttggtaaa
     8161 ggtgacatgt tatacgttgg aaatggtgat tcatcacaaa cacgtattca aggggcgttt
     8221 ttaagtgacc aagaggtgca agatgttgta aattatgtag tagaacaaca acaggcaaat
     8281 tatgtaaaag aaatggaacc agatgcacca gtggataaat cggaaatgaa aagtgaagat
     8341 gctttatatg atgaagcgta tttgtttgtt gttgaacaac aaaaggcaag tacatcattg
     8401 ttacaacgcc aatttagaat tggttataat agagcatcta ggttgatgga tgatttagaa
     8461 cgcaatcagg taatcggtcc acaaaaagga agcaagccta gacaagtttt aatagatctt
     8521 aataatgacg aggtgtaaaa aaatgtcaga aatgaatgcg gtatataacg ttaaacaata
     8581 cattttaaat ttgattaagc aaaataaatt ggaatatggt gaccaacttc caagtaattt
     8641 atcaattgcc agagaattaa atgtaaaaac cgacgatgtt tatgaagcaa ttcaggcatt
     8701 gattactgaa caagtcatta aagataattt tgaagagggc acaagtgtta agtcactgct
     8761 ccctttcttt tatccattga atgaacttat aagtattggg caaatgatta aaaatgcagg
     8821 atttgaatgc ggaactgaat acttaaattt tgatgagcaa ccagcaacta tgttagatgc
     8881 aaatttgttg agtgttgaag aaggatatcc agtaaccatt atagaacgat tacgaactgc
     8941 cgatggagaa ccggtcgtct attgtttaga taaaattgct aaaaaagaat taacatgtac
     9001 agagtatcaa atgagcaatg gatcgatact aagtgcgata aaagaacaaa gtaatcataa
     9061 tatttgttac gctgatacag aaattgaagc ggtaaattat gaacctcgaa tatccgaagt
     9121 actgaatgct tcgccacacg aaggtttgat tttattaaaa attacgcact acaatgaatc
     9181 agatgaacct attttgtatt cattaaatta tatgaaaaat agcttagttc aatttaaaat
     9241 cactagaaaa atatagatgt aagaagatat agaggaggct atatatttga gtagacaatc
     9301 tcaaccaaat atacatatca aagtttcacc aacaactaaa tttaaaacaa ctactatagt
     9361 ttttaaattt atggcacctt tagaatatga cacaataaca gctagatcat tattaagcaa
     9421 attattagtt cgagcaacta agaaatggcc aaccgataag tcatttaata atcacttagc
     9481 cgatttatat ggtgcgtatg tgaatagtac aatttcaaaa ttcaaagatc agcatgtcat
     9541 tacattttca ttagaaattg ttaatgaacg ttatttaaga aacggtgaat cattatttaa
     9601 tcaaggatta gatttattac aagaaatcat ttggaatcca ttaattgaaa ataaagcatt
     9661 caatgataat tttgttaacc aagagaaaac attattagcc aaaaaaatag aagcaatggt
     9721 agataacaaa gcacaatatt cgtttttaaa attactcgac catatgtttg aaaatgaagc
     9781 atataaatac ttatctacag gacaactaga acaaatccca catattactg ctgaaacact
     9841 atatcataca tatcaatcaa tgattaataa tgatcaatgt tctgtttatg ttgtcggcaa
     9901 tgtagaacct gaaagtgttg agaaacaaat acgtgaaaaa tttgcactta aaccattcga
     9961 taaacatcaa ttccaacatt ctactcatca tttacacgat gaagaagttg attatattgt
    10021 tgaatatgat gacgtggatc aagctaaatt aaatatggga taccgatttc caacacaata
    10081 tggacaaagt ggatatgctg cctttgttgt atttaacatg atgtttggag gagatccttc
    10141 atctgtttta tttaatgaag tgcgagaaaa gcaaagttta gcgtactcta tacattcaca
    10201 aattgatggc aaaaatggct atttatttgt tttgagtggg gtttcaagcg ataagtacga
    10261 aactgcaaaa gacactatta taagtgaatt tgaaaaaata aaagcaggag atttcactga
    10321 agaaaaatta gagttagcta aaaaagtaat catttctcat cgatatgaat ctgaagatcg
    10381 tccgaaaagt attatagaga ttatgcataa ccaaatatta ttagagcaac cacaaagcaa
    10441 agaaacattt ataaatgata tacagaaggt aagtcgcgaa gatattgttt ctgttgctga
    10501 aaaagcattt ttagatacaa tctatgtgtt gacaaaagga ggggataaat aatgaaagag
    10561 cgttattatg aattaataga cgaaagagta ttcgaacaag aattagaaaa tggtttacga
    10621 ttatttatta tccccaaacc aggttttcaa aagacatttg tcacttacac tacacaattt
    10681 ggttcattag ataatcaatt caaacccctt ggacaagacc aatttgttac tgtacctgat
    10741 ggagttgctc actttttaga acataaatta tttgaaaaag aagaagaaga cttatttact
    10801 gcgtttgctg aagaaaatgc acaagcaaat gcgtttacaa gctttgatcg tacaagctac
    10861 ttgttcagtg caactgataa tattgaaaac aacattaaac gtttacttac aatggttgaa
    10921 acgccttatt ttacaaaaga aactgttgat aaagaaaaag gtattattgc agaagaaata
    10981 aaaatgtatc aagaacaacc tggatataaa ttaatgttta atacattgcg tgcaatgtat
    11041 caacaacatc caatacgtgt tgatattgcc ggtagtgtag aaagtatata cgatattaca
    11101 aaagatgatt tgtatctatg ttatgaaacg ttttatcatc catcaaatat ggttttattt
    11161 gttgttggtg atgtggatcc tgaagcaata tgtcgaatag taaaacaaca cgaggatgct
    11221 cgtaataaag ttaaccaacc caaaatcgaa cgaggacttg ttgatgaacc ggaggatgtt
    11281 aaagaagcat ttgttactga atctatgaaa attcaatcac caagactaat gcttggtttt
    11341 aaaaataaac cattacaaga agcgcctcaa aaatatgtac aacgtgattt agaaatgtca
    11401 ttattctttg agttaatttt tggggaagaa acagattttt atcagaattt attaaacgaa
    11461 ggacttatcg atgatacatt tggttatcaa tttgtactag agccgacgta tagtttttca
    11521 atcgtgacaa gcgctactga agaaccagat aaattaaaaa aattattatt agatgagttg
    11581 cgcgataaaa aaggcaattt ccaagatgca gaagcatttg aacttttgaa aaagcaattt
    11641 ataggtgaat tcatatcaag tttaaactca cctgaatata ttgctaatca atatactaaa
    11701 ttgtattttg aaggtgttag tgtgtttgac atgttagaca ttgttgaaaa catcacgtta
    11761 gatagtatta atgaaacgtc atcgttatat ttaaatttag atcagcaagt cgatagtcgt
    11821 ttggagatta aaaagtaatg aaagcattag tattaggtgg ttctggttca attggttctg
    11881 agatagtcaa acaattatta actgatggat ttgaagttta tgtgcaatat tatcgtactg
    11941 atataaatga attaactaac aaatttaatg atgataaagt tcattttata caagcggatt
    12001 tatctcaaac aattgatatt gacaaaacat ttggtgacat taaatcatta gactgtttaa
    12061 tatatgcaag tggtcagtct ttatatggtg ttttacaaga tatgaaagac catgatattg
    12121 atgcatgtta tcagttaaat gtcttgcaat taattcgatt atgtagatat ttcgttgatg
    12181 ttttacgtca aagtgacaat ggaagaatta ttgtaatttc atcaatttgg ggtgagacag
    12241 gagctagcat ggaaactatt tattcgacga tgaaaagtgc acaattaggt ttcgttaagg
    12301 cgcttagtca agagcttgca ctaacatcag tgacagtaaa tgctatcgca cctggttttg
    12361 tagctggtaa tatggcaagt gagtggcaag aagatgaact tcaagcaatg ataactgaat
    12421 taccacaaca gcgattggtt ttaccgagtg aggttgctca tacatgcgcc tatttatatc
    12481 acccaaatgc tagaagtgtt actggaacta tacagaaagt taatggggct tggtatattt
    12541 aaaatataga actattttta tgagcaaatg tttttatgat tgatgttata atatgaatat
    12601 tacaatgaat cataatttgt taattaaaca aggggtgagg ttcaccatga cagttgcaga
    12661 gaaaaaagaa tggtacctag aatacgaaat tgaaattaat agaccgggtc ttttaggtga
    12721 tgtatctagt ttattaggta tgttaggtat aagtattgtt acaattaatg gtgttgatca
    12781 aggtaaacga ggccttttaa ttaaaacaga caatcttgaa aaagttgaac gttttgagca
    12841 aatagctcgt ggtataaatg aaattgaaat aacaaagctt aaaaaaccag aattaagaga
    12901 ccgtcttgca gttagacatg gtagatatat tgagcaagat gcaaaagata agaaaacctt
    12961 tcgatttgag cgtgaagatt taggcttgtt agtagacttt ttagctgaat tgttcaaaga
    13021 agaaggtcat aagttgattg gcattagagg tatgccacgg gttggtaaaa ccgaatcaat
    13081 tgttgcggga agtgtttgtg cacataagag atggttattt attagttcta ctttaataaa
    13141 acaaactgta cgtagctctc taattaaagg ggaatatgat gccaatcatg tatatattat
    13201 tgatggtgca gttactgcca gagaatctaa tccaaaacat caagagcttg ttaacgaagt
    13261 tatgacgtta ccttcaatca aagtcgttga acacccagat ttatttgttg aaacaagtac
    13321 ttgtacaatg gaagattttg actatattat cgaattgaga gaaaatgaaa atcaagaaat
    13381 acattacgaa gaaatgaaga aacagacagt ccaaagtaag aataatttag attttggaga
    13441 tccatttggt ggtggttttg gtttcttcga gtaagtgtaa ggaggctatg aattgaaaac
    13501 ggtcggtgaa gcgctaaaag gtagacgtga aaggttagga atgactttaa cagaattaga
    13561 gcaacgtact ggaattaaac gtgaaatgct agtgcatatt gaaaataatg aattcgatca
    13621 actaccgaat aaaaattaca gcgaaggatt tattagaaaa tatgcaagcg tagtaaatat
    13681 tgaacctaac caattaattc aagctcatca agatgaaatt ccatcgaacc aagccgaatg
    13741 ggacgaagta attacagttt tcaataataa taaagactta gattataaga gtaaatcaaa
    13801 agagccaata caattattag taatcatggg tattacagtt ttaataactt tattgttatg
    13861 gatcatgtta gttttaatat tttaacagaa ataaattagt gagaaatgag gatgtataat
    13921 gaatattccg aaccagatta cggtttttag agtagtgtta ataccagttt ttatattgtt
    13981 tgcgttagtt gattttggat ttggcaatgt gtcatttcta ggaggatatg aaataagaat
    14041 tgagttatta atcagtggtt ttatttttat attggcttcc cttagcgatt ttgttgatgg
    14101 ttatttagct agaaaatgga atttagttac aaatatgggg aaatttttgg atccattagc
    14161 ggataaatta ttagttgcaa gtgctttaat tgttcttgtg caactaggac taaccaattc
    14221 tgtagtagca atcattatta ttgccagaga atttgccgta actggtttac gtttactaca
    14281 aattgaacaa ggatttgtaa gtgcagctgg tcaattaggt aaaattaaaa cagcagttac
    14341 tatggtagca attacttggt tgttattagg tgatccattg gcaacattga ttggtttgtc
    14401 attaggacaa attttattat acattggcgt tatttttact atcttatctg gtattgaata
    14461 cttttataaa ggtagagatg tttttaaaca aaaataaata tttgtttata ctagatttca
    14521 ttttcatatg gaatctagtt tttttaatcc caattttaga aattagccac gcatttgttt
    14581 ataatgatat attgtaaaat aatatttgtt cattttttag ggaaatgtgt tgtagcatct
    14641 gatacattga atttaaaatt gatgtgaatt tttaaatgga atacatgaaa aaatgaatta
    14701 aacgatacaa gggggatata aatgtcaatt gccattattg ctgtaggctc agaactattg
    14761 ctaggtcaaa tcgctaatac caatggacaa tttctatcta aagtatttaa tgaaattgga
    14821 caaaatgtat tagaacataa agttattgga gataataaaa aacgtttaga atcaagtgt
//
LOCUS       DAMZPP010000072        14531 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMZPP010000072 DAMZPP010000000
VERSION     DAMZPP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14531
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.072"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..68)
                     /locus_tag="POH73_001621"
     CDS             complement(<1..68)
                     /locus_tag="POH73_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimJ/RimL family protein N-acetyltransferase"
                     /protein_id="HDI6887570.1"
                     /translation="MFGMKVNEQIRLKILEAHDTEAL"
     gene            428..1423
                     /locus_tag="POH73_001622"
     CDS             428..1423
                     /locus_tag="POH73_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HDI6887571.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFKGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSRLLAIDVGVVI
                     FSIVMMLFVNKLLRGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSVLLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            complement(1483..2049)
                     /locus_tag="POH73_001623"
                     /pseudo
     CDS             complement(1483..2049)
                     /locus_tag="POH73_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            complement(2063..3124)
                     /locus_tag="POH73_001624"
     CDS             complement(2063..3124)
                     /locus_tag="POH73_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDI6887572.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPFKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGVFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNTLPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAKTTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            complement(3138..4064)
                     /locus_tag="POH73_001625"
     CDS             complement(3138..4064)
                     /locus_tag="POH73_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6887573.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGI
                     KSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPQMNDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            4376..5734
                     /gene="glpT"
                     /locus_tag="POH73_001626"
     CDS             4376..5734
                     /gene="glpT"
                     /locus_tag="POH73_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDI6887574.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLVLT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYILIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            complement(5818..6258)
                     /locus_tag="POH73_001627"
     CDS             complement(5818..6258)
                     /locus_tag="POH73_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HDI6887575.1"
                     /translation="MYKSKILLKYIFSEESDVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKAGYFVTCWTKDENNCNQPYSKEAFADYLMIIVIDEELSGYFLFPRELLV
                     EKGILTTFEHKGKMAFRVYPKWCNQLNKRAEQTQKWQCKYFFEY"
     gene            complement(6364..7719)
                     /gene="mepA"
                     /locus_tag="POH73_001628"
     CDS             complement(6364..7719)
                     /gene="mepA"
                     /locus_tag="POH73_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HDI6887576.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVALPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMISMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMSNKGRMKDVIKAVIMSIGVIFVVCMIAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMTSLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGVVIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            complement(7826..8245)
                     /gene="mepR"
                     /locus_tag="POH73_001629"
     CDS             complement(7826..8245)
                     /gene="mepR"
                     /locus_tag="POH73_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HDI6887577.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            8457..10412
                     /locus_tag="POH73_001630"
     CDS             8457..10412
                     /locus_tag="POH73_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDI6887578.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTANTTILNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHIRIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAITVAVQPLINDGIVNSNYTAAILKQLEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLCRQDFLDGNIFKIKQHIALTMTKEV"
     gene            10417..10860
                     /locus_tag="POH73_001631"
     CDS             10417..10860
                     /locus_tag="POH73_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6887579.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            10862..11146
                     /locus_tag="POH73_001632"
     CDS             10862..11146
                     /locus_tag="POH73_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI6887580.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            11161..12507
                     /locus_tag="POH73_001633"
     CDS             11161..12507
                     /locus_tag="POH73_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDI6887581.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAVAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATAILLKGRQKPQQ"
     gene            complement(12601..13626)
                     /locus_tag="POH73_001634"
     CDS             complement(12601..13626)
                     /locus_tag="POH73_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HDI6887582.1"
                     /translation="MNNKVLVTGGTGFVGMRIISRLLEQGYEVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAQHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQASGKRFIATADGQIDLLEIAKLIKEKRPEIAQKVAT
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAIDS
                     MAKFHLI"
     gene            complement(13942..>14531)
                     /locus_tag="POH73_001635"
     CDS             complement(13942..>14531)
                     /locus_tag="POH73_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDI6887583.1"
                     /translation="NNRIYGGYSLLLDVNYEAMDKVLKPNRKKIASKGIKSVRARVGH
                     LREALDEKYRDITIEEFKNLMVTQILGIDDIKEAKRYELSDADWESIEELASKKYKNW
                     DWNYGKSPKYEYNRSERLSSGTVDITISVEQNRIADCRILGDFFGQGDIKDVEQALQG
                     TKMTREDLTHQLKQLDIVYYFGNVTVESLVEMILS"
BASE COUNT         4604 a         2748 c         2243 g         4936 t
ORIGIN      
        1 agcgcttctg tgtcatgggc ttctaaaatt tttaatctta tttgttcatt cactttcatt
       61 ccaaacatac catcacaccc tcatttattt ttcatataat tctgtaaatt atcataataa
      121 taacatatta atattacgcc ttgtatctta aatttgttgt ccttccccaa cttgcattgt
      181 ttgtagaatt tcttttcgaa attctctatg ttggggcccc gccaacttgc actgtttgta
      241 gaatttcttt tcgaaattct ctatgttggg gcctcgacaa taagcattgt agtgcctatt
      301 actttggttg atgcccttga tacctatcac acaacttaat ttaatcacta ctttattaac
      361 cattccaaat ttatatttaa aaatacgaca aaacagtgct aaaatgctat taaaggaggc
      421 actgctcatg gcatcattga aaaataagca ttttatgatt ggactatcat taacatttat
      481 agtcgcatta tttagctttt tagcagctaa attgccaatc ttagataagg ttggcgcatt
      541 aactatcgcg atattaatcg ctattctgta tcgtcacttt aaaggatatc ctgaacaata
      601 tagctcaggt attacatttt catccaaata tttattaaga tttgcaatca tcctatatgg
      661 acttaaactc aatatattcg atattatcgg tcaaggtagt agactattag ccatcgatgt
      721 aggtgtcgtt attttcagta ttgtaatgat gctctttgtc aacaaactgt tgcgtggtga
      781 caaaaacatc gcattattac taggtgtagg tacaggcgtt tgtggtgctg ccgctattgc
      841 tgcagtagct ccaatattca aatcacgtga aaaagataca gctattagta tcggtatcat
      901 cgcattgatt ggtacgatat tttcacttat atatacagct atctatgcta tcttttcaat
      961 gacgacaaat gtttatggcg cttggtctgg cgttagtctt catgaaattg cacacgttgt
     1021 cttagctggt ggctttggtg gtagtgatgc acttaaaatc gcacttcttg gtaaacttgg
     1081 tagagtattc ttactgattc cattaaccat cgtacttatt ttaattatgc gcttccgttc
     1141 atcagaatca tctagcaagg gtcgaataag cattccatac tttttaattg gatttgtcat
     1201 tatggcctta gtgaatacat atgtaactat cccgtcagta ttgttaaata ttttaaatac
     1261 tgtatcaaca atctgcttat taatggccat ggttgcttta ggattaaatg ttgcatttaa
     1321 agatcttaaa aatcgtgcac ttaaaccgct tatgacaatc attattacct caatatgctt
     1381 gtcatcactt gcctttatcg tcgttcactg gctatatagt taatatgaaa agacgctcaa
     1441 acacttacaa atttagtgtg ccgagcgtct tatttttata ctttaaaagt cgtattggtt
     1501 attttcttct tcaagcactt gttgttgtac tttggcagtt cgcattgtgg attgtgccaa
     1561 cgcttttaaa cgaaatacaa catcatcatt gacaatttgt tgatttgaaa agtctttctc
     1621 ttcaatgaat acatacgttt gcatcgtatg tgctttcatg taactcaata ttggttttaa
     1681 atgcatttca ggaattaaat aatgtttact agaacctgct gtcgctacaa gtcctattac
     1741 cttatcatga aacgcattga ctggaagtaa atcaaacaca tttttcaaag aaccagggat
     1801 ggaagcttga aaaattggaa aaccaataaa aatcacatca gcctgcatta acgacgtcgt
     1861 taatttatat acatctcctg tagtatctag ataattcttt ccaacactaa attataattc
     1921 gagatctcgt aaatccatca atgctatttg gtgtccctca ttgatgactt ctagttcatt
     1981 ttttaaatca tccatagcaa ttctcgtttt agaacctact gtggagcctg acaataatac
     2041 aatattcatg atgtcgcccc tcctattttg ataaatgctt tttaatcgct ggtataatgt
     2101 cgttgccaat taactcaata ttcttcataa cattttcaaa tggcacaccg ccaaaatcaa
     2161 gctgtgccat aaaacgttga tgaccatata actcgtgttg atacaatatc ttttcaataa
     2221 tttgttgcgg gcttccaacc attagcgctt ctcgataatc tgacgaatta gcaaattgct
     2281 gtttcggata accaacgcca cgaataaatg acatccctgt attcaaatgt ggataaaatt
     2341 ctctcatagc atcctgagtt gttttagctg tataaaacag actcgctgta cttactggta
     2401 aagacttagg cgaagcatcg aaacctgctt cagttgctgc ttgacgataa gcatctatag
     2461 aacctttaaa gttcattgct gggccaccaa gggttgtaat catcattggc acaccttgtt
     2521 ttcccgcttt gatagcactt gcaggtggac caccaacagc acgccatatt ggcaatgtat
     2581 tatcgattgg tcttgggaat attttcatat ttctaagttc tggacgatat tttccagacc
     2641 aagtaatacg ctcagtttta tttaactcta aaagtaaacc taatttttct tcaaacaatt
     2701 catcatagtc ttttaaatca tagccaaaca agtcaaaaac acctgttctt gatgctctgc
     2761 cagctacaat ttcggctcta ccatgagaaa tcaaatctaa tgtcgcgaag tcttcaaata
     2821 ctcttacagg atctgttgca ctaataatcg ttgaagaact tgaaacttta atatgctttg
     2881 tcgcttgggc aattgcacct aacacaaccg tatgtgcctg tgtcgtaaaa tgttcctgat
     2941 gactttcacc aactgcaaaa acatcaatac ccgcttcatc tgctaattta cttgcttcaa
     3001 taatttcatt aatacgttgc tcataactaa ctttttcacc tttaaatgga ttcaataaat
     3061 gatcacctaa ggaatacaaa ccaaactcaa gaggcgtatt tttattcatt tctaattttg
     3121 ccattaaaca ttctccttta tatttcgtgt aacttacttt caatttgttc acgttctgct
     3181 tctaaaaagt caggcaagtt caattgactt cctaattgtt caacaggtgt atcaatagta
     3241 aatccaggcg cttcagtcgc aaattcatac ataattgaat tatggcgata gtataatgat
     3301 ttaaagaaat agcgatcaat tatacctgaa ttactttgtg gttgttgttg tagtttcttg
     3361 taaattgcat ctaagtcatt catttgtggt gtattaactg caatatgatg gatataacct
     3421 cgtccaggtc tttcacgttg tccctgttgc tcaataacta caaaatccga atataaacct
     3481 tgtggtgcta atgtcagcac atcgaatgat ttattatctc ttttgcgata acctaagaca
     3541 ttttccaaaa attctaccgt acgcgctgca tttctaactc ttaattcgac tggccctatt
     3601 cctaaaattt gatatgcttg aggtatttca ctataagcgt tatgttgcca ttgatgtggt
     3661 acttcaaaac tatcattaac aagcagtatg atttctaagt cgtcttcatc tttaaaaaca
     3721 acaccttctt gtccaagata gtacaaacgt tcagacttaa taccattatt ctcaagacgt
     3781 ttttcaaaat aatgaagtgc cgcttgattt ggtactaata atgaaaatcg ataaatcgtt
     3841 tcagtacctg gctgcttatg acccgcattg ggaatttcaa aaaaacttaa aattgttccg
     3901 gctgtaccta cttcgtcccc ataaaacaaa tgatacattg aaggattgtc ttgattaacc
     3961 gacttttcaa ccaatcgtaa tccaaggaca tttgtgtaaa aatccttatt acgttttgca
     4021 tcttttgtat acatggatat gtgatgatgc cctactatat tcatactatc cctcctatta
     4081 gtataaattt tataccaact tatataaaaa agatagttat atgccattta gcccttttct
     4141 gcaaataaaa atatgtttgc gctccttcgt agatacacag ctagtatgta taacgacata
     4201 ataattgcat aattataaat ttatgtgtag caatttattt tttctaaatg tttataaaat
     4261 acttcaataa ctaaatatgt aagatttcat aaaagtatat tgacaacgct ttcatacatg
     4321 tctaatataa gtttatcgca caaaatgcat ataaatacat attaaggagg attttatgaa
     4381 ttttcttaaa cctgcaaagc atattaagcc tttgccagaa aatcagatag atgataccta
     4441 taaacgatta cgtctccaag tatttcttgg tattttcatc ggttacgctg ggtactattt
     4501 attacgtaaa aacttttcat tagcgatgcc ggcattgcaa gagcaaggtt ttacaaaagc
     4561 agaactaggt tttgcgcttt ctgctgtttc catcgcatat ggatttagta agttctttat
     4621 gggtactgta agtgatcgga gcaatgcacg gatattctta gttcttggat tagtactcac
     4681 tgctatcgtc aatttgttaa tgggatttgt accgttcttt acatcaggta tcggtattat
     4741 gtttgtccta ttattcttaa atggatggtt tcaaggtatg ggctggccac cttcaggccg
     4801 tgttctcgtt cactggttta gtgtaagtga acgcggaagt aagactgctc tttggaacgt
     4861 tgcgcataat gttggtggag gtattatggc acctattgct gcttggggta ttacaacaac
     4921 agcatttatc aactttggtt atttaaaagg ttttgaaggt gtattcattt accctgcact
     4981 cttagcactt atcattgccg caatttcata tatattgatt agagacacac ctcaatctca
     5041 aggtttacct ccaatcgaaa tttataaaaa tgactttgct acaagcgata agaaaacatt
     5101 agaaacagaa ttaactacaa aagaaatttt atttaaatat gtactgaaca ataaatgggt
     5161 atgggcaatt gcctttgcaa atatatttgt ttatttcgtg cgttatggtg tacttgattg
     5221 ggcgccagtc tacttaagtg aagaaaaaca tttcgactta aaagcatcag gttgggcata
     5281 cttcttatac gaatgggctg gaattcctgg tacattatta tgtggttaca tttctgataa
     5341 attattcaaa ggtcgccgtg gacctgctgg tttcttcttt atgttaggtg tcacagtatt
     5401 tgtattaatt tattggttaa atcctccagg caatgcttgg ttagacaatg tctcattaat
     5461 tgccattggt ttcttaatat atggaccagt tatgttaatt ggtttacaag cattagatta
     5521 tgtacctaaa aaagcagctg gcacagcagc tggattaact ggattatttg gttatctgtt
     5581 tggtgctgta atggccaaca tcgtcttagg tgctgtagtt gataaattcg gatgggatgt
     5641 cggttttatt ttattaacag caatcagtgt gtttgcaatg ttgagcttta tcctcacttg
     5701 gaataaagta ggacaagaaa ctgttcatca ttaaatgata aaaaataaag tcatatggtt
     5761 atcttatcga aagatgatat atacatctct tataagttca accatatgac tttttaatta
     5821 gtattcaaaa aaatatttac attgccactt ttgtgtttgc tctgctcttt tattcaattg
     5881 attacaccac ttaggataaa ctctaaaagc cattttacct ttatgctcaa atgtagttaa
     5941 gatgcctttt tctaccaata attccctagg aaataagaaa taaccgctta attcttcatc
     6001 aataactata atcattaagt aatctgcaaa tgcctctttt gagtatggtt gattacaatt
     6061 gttctcgtct tttgtccaac atgtaacaaa ataccctgct ttagtcggtg ttttctttgc
     6121 taacctactt tgatatgttt cctctttaaa gctaaatgtt aatgcttcgt aatcttgatt
     6181 atatttttct tccgttaaat ctttaacgtc tgattcttca ctaaaaatat atttcaacag
     6241 tattttagat ttatacatat gccaatctca ctttattttt tcataatcgt atcatatatt
     6301 tattttttat ttgaaaaata cacttccgac cgaaatatac tcagccagaa gtgtacgaaa
     6361 tatttaacct tctattaatt cagatgtatc aactgtcaaa cgatcacgta ataaatagac
     6421 gattaacatt gctgctaaag cacaaagtga ctcagcaatt aataatgacc aaatgacacc
     6481 tgttagtcca aacaaagcat tcataataaa tagtactgga atgataacca caccttgtaa
     6541 aatggccata attgtagcac cgcgaccttg accagttgct tgaagcatac cagtaaacaa
     6601 gaaacctata ccatttaata acaatgatgt cattgttact ttcaaaataa atgtcgccat
     6661 ctcaacaatg gcttgatcag tagtaaatag tccgaccata tgatgtccaa ttgtaaatac
     6721 cgcaatcata catacaacaa agataacgcc gatagacatg ataactgctt tgataacgtc
     6781 tttcatacga cctttatttg acataaagtt atatgcaatt aatggtacaa caccttcaca
     6841 taagcccatg ataataagtt ctggaaattg cacaagtcta aatgagatac cataacttgc
     6901 aatcgcgaag tttccataat gtgctaaaaa taaatttaaa actaatcctg tgaatcccat
     6961 taagatactc attaaaaatg caggaatacc gattttaaag atttcagaaa gcatttcttt
     7021 attaggtttc gcaagtttaa tattaactga cacaacgtca ctatttttca taaaataaat
     7081 gataaagaac agagcagcag caacattact gattgcagta cccaaagctg caccaacaac
     7141 gtttaaatca aaaccaaaaa ttaaaattgg atctaaaata atatttaagc ctacactagc
     7201 taacatacca atcatagaaa tcattggtgc cccaattgca cgtgcaaatt gttctaatat
     7261 gaagaacaaa attacaaaag gtgcacttaa aaacattact ttcaaataat tacttgttaa
     7321 agctaacgtt tcacctctcg cccctaaaat tgctgcgatt tgatcactga atggtaaagc
     7381 aactaaaatt acgataagtc ctagtgcaat accaccataa atagagaaac tacttacaaa
     7441 tttactctta ctatagtctt tcgcacctaa taaacgtgaa atataagttc ctgcaccaac
     7501 gccaaataaa ttacccaacc ccattaagat agcaaatact ggcagtgtta gtgagatagc
     7561 agaaatcatg tggctatctt ctaaaaatcc tataaagtaa atatttaata tgccataaat
     7621 aacgcttaat aaagtcccta tcatcattgg caatgagaaa tgcatcatcg ctttaaatac
     7681 tggcgatttc tcaaaataat ataattgttc gtctttcatt gttcaatact ccttgtcttt
     7741 tccaataatt agcttactaa caattagata tctaactata acattaaaga taaagtgact
     7801 gatttctacc agtcacgctt accatttatt gtaaactaga taacatttta gttaagtttg
     7861 ctttcatctg ttcattttct tcttcagata actgcgatac gagtgtttgc tccatttcat
     7921 caaatatcga agtgaatgct tctacaagtt taatcccaga ggtagtcagt cctatattct
     7981 ttcttctcgt atcctgtgca tcgacatagc gatagatcag ctttttacgt tcaaggttcc
     8041 ttaataaatt actgacagtt ggacctgttc gttgtaatgc tttagcaata tcattttgtg
     8101 tcagcccatc ttgttgatgt gcataaagat aacctaacgt atgcccttgc tcatttgtaa
     8161 tatcaaattg ctctaacttt tgatcagcct tttgtttcat ctcatgacta atcattctaa
     8221 ataaatacga ataagtgaat tccatttatt gcaccaccat ttcgttagat gtctaactaa
     8281 atatactgcg ccatttttaa attgtcaaca tgaatatcta atttatttta aaagttccta
     8341 taaaatcttg cgccataaac attttgatat gcaatttttc ggattaaaat atgaaagaga
     8401 ttgcatatct acgttcttga gaaatcgctt acaattacaa ataggaggat attacgttgt
     8461 taagttatag acaaattgaa attttgtatc ttttaattca tgagcaaacg tttattccaa
     8521 tcaataccat tgctaatcaa cttggtgtat cacctcgtac cattcaatac gatatagcat
     8581 atattgagca atatgctgaa acttatcatt accaagtcag ccgtaataaa gctgcaggca
     8641 tcaaagtaac aacagctaac acaacgatat taaatgaatt agagcacaat ctaacgaatc
     8701 aaattcattt ttcaaaagat gagcgactca cacatatcgc tttaaagtta ttcgaaacaa
     8761 ctgatcctgt ttcaacaaag caacttgcgc aagatgttaa tgtttcgcgt cggacaattg
     8821 cagatgatat taaaatgatt caagcccaac tagatcaata ccatttaaag ttaaattatg
     8881 tccacaataa aggattcaat attattggtg aggaagatca ttatcgtaaa gcgtatgcac
     8941 actttataca tcaatatatg aaacaagctg caccttttat agaggctgat atctttaatt
     9001 cagaatcaat cgcattagtt cgccgtgcca ttattaagac attaaatagt gaaaattatc
     9061 atttagttca gtcggctatc gatggcttaa tctatcatat acgcattgcc attcagcgtt
     9121 taaatgaaaa tttttcgttc gatataccta tcaatgaaat tgataaatgg cgacatacta
     9181 atcagtatgc tattgcttca aaaatgatag aaaacttaga acgcagttgt aatgtcacat
     9241 ttccagagtc tgaaattatc tttatcacgt tacatttact aggctctaaa atgaccgaac
     9301 atacagcatc ttcaattacc tttgaatacc atgatttatc gcaaaatata catgaattga
     9361 tcacttgtgt tagccaagaa ttaggcattg atatgtcaaa agacaacaag ttgcatacca
     9421 gtcttatcac acatatcaaa ccagctatac atcgtattaa atacgatatg ctacaaccta
     9481 atcctttgag gcaagaagtt atgcgtcgct atcctcaaat cattgaagcc gttagcaagc
     9541 atattagccc aattgaacaa gatgctgcta ttcgcttcaa cgaagatgaa ttaacataca
     9601 ttacaattca cttcgcatca agtatagagc gtgttgcaac acataaacaa tcaatgatta
     9661 aggttgtctt actatgtggt tctggtatag gcacgtcgca acttttaaaa tcaaaactaa
     9721 atcatctgta tcctgagttt cacatttggg atgcctattc catttatcaa ttggaagaaa
     9781 gtcgattatt gcaagataac attgattatg tcatttcaac agtaccttgt gaaatctcag
     9841 ctgtaccagt tattcatgtc gatccattta tcaatcagca atctcgtcaa aaattgaatc
     9901 aaattatcaa tgactcaaga gaacaacgag tcatgaaaat ggcaactgat ggcaagtcac
     9961 tcgcagattt attgcctgaa catcgcatca ttataaataa acaaccatta tcaattgaat
    10021 cggcaattac agtggctgtg caacctttaa tcaatgatgg cattgtcaat tcaaattata
    10081 cagctgcaat tttaaaacaa ttagaacaat tcgggtcata tatggtcatt agtccacaca
    10141 ttgcacttat tcacgctggt actgattatg tacagaatgg tgtaggtttc gcactaactt
    10201 atttcactga agggattatt tttggtagta aagctaacga tcccgttcac cttgtaatta
    10261 cattagcaac ggaccacccc aatgcacatt taaaggcatt gggacagtta agcgaatgct
    10321 taagcaacga cttatgtcga caagatttct tagatgggaa tatttttaaa attaaacaac
    10381 acattgcttt aactatgaca aaggaggttt aataacgtgt cattagacat tttgtcaaca
    10441 acacgcatca ttgtaaaaga acaagtaaat gattggactg aagctataac tatagcttct
    10501 cagccattac tacaagaaca aattattgaa caaggctatg ttcaagcaat gattgatagc
    10561 gttaatgaac ttggacctta tatcgttatc gcacctgaaa ttgcaattgc acatgcaaga
    10621 ccgaacaatg acgtacatca agttggttta agtctattaa agttgaatca acatgtggca
    10681 ttttgtgatg aagatcacta cgcatcactc atttttgtat tgagtgccat cgacaatcat
    10741 tcacacttat ctgtattaca aaatttagca accgtactgg gcgataacca aacagtccag
    10801 caactattaa ctgcaacaaa cgcacaagac attaaaaaca ttttaaagga gcatgattaa
    10861 tatgaaaatt ttagtagtat gtggccacgg tttaggaagt agttttatgg tagaaatgaa
    10921 tgcacaagaa gcgcttaggc aacttaatgc accatctgat atcgaagttg aacatagcga
    10981 cattatgaca gcaagtccag agatggctga cttgtttatt tgtggtagag atttagctga
    11041 aaatgccgaa cgtctagggg atgtcttagt tcttgataat attttagaca aagctgaatt
    11101 acaacaaaag ctctcagaaa aattacaaca acttaacatg atttaaagga ggtacgacct
    11161 atgcaagcaa tccttaattt tatagtcgat attttaagtc aaccagccat tcttgttgca
    11221 ctgattgcct ttataggttt aatcgttcag aaaaaacctg ccgcaacaat cacttcagga
    11281 accattaaaa cgatattggg cttcttaatt ttaagtgcag gtgctgatgt cgtcgttcga
    11341 tctcttgagc cattcggcaa aatattccaa cacgcattcg gtgtgcaagg tatcgtacct
    11401 aacaacgaag ctatcgtctc actagcctta aaagattttg gaacaacagc tgcactcatc
    11461 atggtctgtg gcatgattgt taatatttta attgcccgct tcactaattt aaaatatatc
    11521 tttttaacag gtcatcatac attttacatg gctgcgtttt tagcaatcat tttaacagtc
    11581 agtcatatta aaggctggct aacgattgtt atcggcgcac tcgtattagg attaatcatg
    11641 gcagtcttac ctgcattact ccaacctacg atgcgaaaaa ttacagggaa tgaccaagta
    11701 gcgttaggtc attttggctc aatcagttac tttgccgcag gtgctgtagg tcaattattc
    11761 aaaggtaagt ctaaatcaac ggaagagatt aaatttccaa aaggcttaag tttcttacga
    11821 gaaagtacaa ttagtatctc gattacgatg gcattacttt acttcatcgc atgcttattt
    11881 gcgggcgtta gttatgtaca cgaatctatt agtgatggtc aaaactttat tgtattttca
    11941 ttaattcaag gtgtgacatt tgctgctggt gtatttatta ttttaacggg cgttcgctta
    12001 atcttagctg aaatcgtccc agcatttaaa ggcatttcag aaaagcttgt accaaattct
    12061 aaacctgcat tagactgccc tattgtgttc ccttatgcac aaaatgcagt actaatcgga
    12121 ttctttgtca gctttattac aggtgtcatc ggtatgttta tcttattctt atttggtggc
    12181 gtcgtcattt tacctggcgt agttgcacac ttcttcttag gtgcaacggc tgctgtattc
    12241 ggtaatgcaa gaggcggtat taaaggtgct gttgctggcg ccgctctaaa tggtatccta
    12301 ataacgtttt taccattatt attcttgcca tttttaggcg aattaggtgg tgctgcaaca
    12361 acattctcag atacagactt tttagctgtc ggtatcgtgt tcggtaacgc agtaaaatat
    12421 atgggattat ttggtgcgat tctatttatt ataatcgtag gtgcaacagc aattttatta
    12481 aaaggtcgtc aaaagccaca gcaatagtgt taacgtaaaa atataaaaca ccgtcacata
    12541 ttgagtgaat gcccctttta tcaagaggaa agccacttac ttatggacgg tgtttttgta
    12601 ttatattaaa tgaaatttag ccatactatc aattgctgct aaaattgctt cttcttgtgt
    12661 cgcaatcggt tcccaaccaa gtaatgtttt tgcacgttcg ttacttacat tgcggttcat
    12721 atctaataaa agtttacctt ctttagcttg atgattaaat ttagcaccta gactcaaaat
    12781 aaagtctggc aattttttag tagcaacttt ttgagctatt tcaggtcgtt tttctttaat
    12841 taattttgca atttccaata aatcaatttg cccatcagcc gtcgcaataa atcgtttacc
    12901 gctggcttgc tcattggtca ttgccaaaat gtgtagttct gctacatctc tcacatcaac
    12961 aacatttaat ggaatttgag gtacacgttt cattgaacca ttcaataaat tttctaataa
    13021 atgaaagctt cctgaaacat gtgcatctaa tgatggtcca aaaattgcaa ctggattgat
    13081 tgtggcaaat tctactgtcg tattttcatt ctcaacaaaa tcccacgctg ctttttcagc
    13141 taacaatttt gatttttcat atactgataa gcctggttca tcttcatttg tccaatgact
    13201 ttcatttgtg attgaatttt tatctttatt actaaaacca actgcaccaa agtttgcagt
    13261 cattaccaca cgtttaacac ccgcatgttg tgcagctctt aaaatacgtt gtataccttc
    13321 aattgcaggc ttcgccatca cttctgcatc gtctgtttta ccgaaaaaca ccggagatgc
    13381 cacactcaac acatatttac aatctttcat tgcttcatcc caatgttcat cttgtgataa
    13441 atccgcttcg acaaacatta atcgctctgt ggaaatgcca ttgtcttgca tcgttttaat
    13501 tactttatca gctttactta aatcacgtat cgtcgtttgt acctcataac cttgttctaa
    13561 taatcgtgaa ataattcgca tgccaacaaa accagtacca ccggtcacta atactttatt
    13621 attcatcaca taatctcctc tttgtgtcta ttgattattc attatagcag tcattatatt
    13681 ttgacgtata taaatagact ttggtagcta ttatttaatc ttacttgtat aaatgaaaat
    13741 gaatttattc ctttaaaaac tatcattaac cattttcaaa tacccaatca ctaataaaca
    13801 caaacgctat aaatatgatg tctttgctaa ttaactaaat tcattttcaa tcgtatgttc
    13861 aaatcacata actacatagt caccatttta aaagcaaata cacatgttca acgagagtgc
    13921 gacattaata cctaaacaat attaacttaa aatcatctca accaatgatt caaccgtaac
    13981 attaccaaaa taataaacga tgtctaattg ctttaactga tgcgttaaat cttctcttgt
    14041 catttttgtt ccttgtaatg cttgttccac atcttttata tcaccttgtc caaagaaatc
    14101 acccaaaatt ctacaatctg caatacgatt ttgttcaaca gaaatggtaa tatctacagt
    14161 acctgatgat aatctttcac ttcgattata ttcatattta ggtgacttcc cataattcca
    14221 atcccaattt ttatactttt tactcgccaa ttcttcaatc gattcccaat ctgcatcaga
    14281 taattcatat cgtttcgcct ctttaatgtc atcgattccc aaaatctgcg tcaccattaa
    14341 atttttaaat tcttcaatgg ttatatccct atacttttca tccagtgctt ctctaagatg
    14401 accaacacgt gcacgcacag atttaatccc tttcgatgca atctttttgc gattaggctt
    14461 taacacttta tccattgctt cataattaac atcaagtaat agcgaataac cgccataaat
    14521 acgattgttc a
//
LOCUS       DAMZPP010000073        14199 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMZPP010000073 DAMZPP010000000
VERSION     DAMZPP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14199
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.073"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            242..595
                     /locus_tag="POH73_001636"
     CDS             242..595
                     /locus_tag="POH73_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDI6887584.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            663..1058
                     /locus_tag="POH73_001637"
     CDS             663..1058
                     /locus_tag="POH73_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDI6887585.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIIVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            1311..1880
                     /locus_tag="POH73_001638"
     CDS             1311..1880
                     /locus_tag="POH73_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6887586.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCDLFNVTLDDLVKGTIPFAPDIKAQRSLNLWTYVMLIFMTLAAILTGPLVVY
                     WNWAWGITVIVIYGIGYYASMKIEDFKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNI
                     KTGVLSFIAFVGAIILIALISMHLANKFL"
     gene            complement(2007..2207)
                     /locus_tag="POH73_001639"
     CDS             complement(2007..2207)
                     /locus_tag="POH73_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDI6887587.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            2691..3494
                     /locus_tag="POH73_001640"
     CDS             2691..3494
                     /locus_tag="POH73_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDI6887588.1"
                     /translation="MSRNLIHLSIGVAIFIISFSFYLLNQPNNWAWSSLNICINDSKP
                     SNVKVAILDTGINNTRINNNFVTKRYNAINNHNQITTDSNHATQVASIITYGEHKEKL
                     IGISKSVSIYDVKVLDDNLKGDIDNIIKGITWSIKNDVNIIHMSFGFQRYNKELHDAI
                     KNAKNKGIILVAAAGNNMDDYSDYPARFNEVYSITAIDKNNKPFIYAPTKKIDFKAPG
                     VDVYTKGSKDELLTVNGTSFAAAYFTAYLTNNLEFRNDLPHILKEYPIK"
     gene            3513..4262
                     /locus_tag="POH73_001641"
     CDS             3513..4262
                     /locus_tag="POH73_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887589.1"
                     /translation="MKNKIIILFLAIIITFTYGNFAEASVVIKEANINNSNSKINTNK
                     INENIYSKNNRNYIDNNFKSKDLNIESSLEHDLNTDKITVDASLKDNYNNKLDKTYDV
                     KFLRIVNENDFKAEFTDQDTGEKIIYDTNEVKASIAPVVAVLVGFIAKQGLKKAIQKY
                     GKTVVTSMIRTSPQVAAQAAKKLGYSATKHVSHGKKVFKKNSKGRPQYISVDKDGHRG
                     GAWKGASSIKNLGSKKTRSGTYDANLKRIGD"
     gene            4264..4761
                     /locus_tag="POH73_001642"
     CDS             4264..4761
                     /locus_tag="POH73_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6887590.1"
                     /translation="MIINLETQQIHFDLEHEIPYFQSPEKNRIRLDIDVLNKKQISNI
                     INVIFNNQSKTKCTFLSEYLYPVKFREKTRIGRFFNITNWYEEIHSADEKYVIATIKN
                     IKNKDLIDYSLYIKKGLVEPFIVFYNQENIFYISNAVIDIISVSKDTLGYIKSEFNDH
                     CASPY"
     gene            complement(4829..5786)
                     /locus_tag="POH73_001643"
                     /pseudo
     CDS             complement(4829..5786)
                     /locus_tag="POH73_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(5849..6040)
                     /gene="vraH"
                     /locus_tag="POH73_001644"
     CDS             complement(5849..6040)
                     /gene="vraH"
                     /locus_tag="POH73_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HDI6887591.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALAIIVIVLSTVTTPIIGI
                     PAGLLGGAYYLKRREEKGK"
     gene            complement(6132..8012)
                     /gene="vraE"
                     /locus_tag="POH73_001645"
     CDS             complement(6132..8012)
                     /gene="vraE"
                     /locus_tag="POH73_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HDI6887592.1"
                     /translation="MTFNHIVLKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFIITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTILML
                     IIAYILILFQSSLFLKRRSILSMMKDSVKTDVTTAKVTVIEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNDFNVVASQ
                     DAQQFESKLSQQHITFSKNYYETITVDNVKDQVITLENGSDSGRTNSILSANTKLTGN
                     NAIITNTKSLPNVINIHLNNDLVVKGTKNETFRVTQEDKDKVYPLNLIFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKKKAQAIAKQFGDKIITYDDMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIIVIIVYTLIYIVFALIAFVHSNKLIKK
                     TI"
     gene            complement(8002..8760)
                     /gene="vraD"
                     /locus_tag="POH73_001646"
     CDS             complement(8002..8760)
                     /gene="vraD"
                     /locus_tag="POH73_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HDI6887593.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            9050..9562
                     /locus_tag="POH73_001647"
     CDS             9050..9562
                     /locus_tag="POH73_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HDI6887594.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGNQNWILFMGIVDILFGLIVIFNVGASSAFFIYMFAFW
                     FIFSSIAGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            complement(9864..10664)
                     /locus_tag="POH73_001648"
     CDS             complement(9864..10664)
                     /locus_tag="POH73_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HDI6887595.1"
                     /translation="MNIAKLEDYLQIEPTRYNQPSIEALNYYATRFMLTVPFENIDVQ
                     NGKPISVDIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     QNGSHASLVVSINNVFYVTDVGFGDLPLNAIPITLPDDTQQITDISGTFRAIFNSEDK
                     DIFYVQKYENNHWHTKYEAEFKAREIEEFDQNIEYNQTHPDSVFVKHLLITMPQSFGR
                     ATMFENHLTLTKDGTPEKLAITKDNYKQFLTKYFGLNVTISRLENSDN"
     gene            complement(10906..11922)
                     /locus_tag="POH73_001649"
     CDS             complement(10906..11922)
                     /locus_tag="POH73_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HDI6887596.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGFVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVIAALV
                     IGMIELLQILADKLDLHGAFWAFISSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSK"
     gene            complement(12086..12661)
                     /locus_tag="POH73_001650"
     CDS             complement(12086..12661)
                     /locus_tag="POH73_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6887597.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEDVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVHSSLPFLLTGDVPCNHAPKRQQMIDFIVATAALDIELLRS
                     LVTTEFIWSVPGHFDIYGPQILIQELSNHYKEIASLNIQSIITHGYFGSMHGSQILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            12883..13791
                     /gene="rarD"
                     /locus_tag="POH73_001651"
     CDS             12883..13791
                     /gene="rarD"
                     /locus_tag="POH73_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDI6887598.1"
                     /translation="MNTEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SVIFMIFILAVGQKQRNAFQRDMNQLLAKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
BASE COUNT         4825 a         2112 c         2407 g         4855 t
ORIGIN      
        1 acaggcaatg cgagttgggg tgggacgacg aaataaattt tgcgaaaata tcatttctgt
       61 cccactccct aatttgagct ggatatacct ttatttgaac cctttattgc tagtttatga
      121 aagtatcatg aaagctttat gaacatcgct tgagttgcct ttacagtaga aaatttaagt
      181 tttacacttt gtgtgaatga tacgttttgt attgaattaa ttatagaaag gtacgttgaa
      241 gatgttttca attggaagtg caattcttca ttttgtcatt ggtggtatcg ctgttgcatt
      301 agcttcaatt attgctgata aggtaggtgg taagttagga ggtattatag ctactatgcc
      361 ggcagtcttt cttgcggcta ttatcgcatt agctttggat catcgtggta cgcaattagt
      421 ggagatgtcg atgaatctta gtactggagc aattgtcggt attctgtctt gtatattaac
      481 tgtatttttg acatctctct atattaagca taaaggttat cggaaaggcg caatattcac
      541 agttgtttgt tggtttgtta tttccctcgc aatattcagt attagacatt tatagtttgg
      601 aaaatgcgtg ataattagtt gtattcagtt attaagtaat aaattattgg aggcagaaca
      661 tcatgaaatt aacattaatg aaattttttg tggggggatt tgcagtatta ttaagttata
      721 ttgtatctgt aacactacct tggaaagaat ttggcggtat atttgcaacg tttccggcag
      781 tatttttagt gtctatgttt attacaggta tgcaatatgg tgataaagtc gctgtgcatg
      841 taagtcgtgg cgcagtgttt ggtatgacag gggtattagt ttgtatttta gttacatgga
      901 tgatgttaca tatgacgcac atgtggttga ttagcattat tgttggtttc ctaagctggt
      961 tcatcagtgc agtatgtatt tttgaagcgg tagaatttat agcacaaaaa agattagaaa
     1021 agcatagttg gaaagctgga aaatcgaata gtaaatagtg tgaacgtaat ctcttaaata
     1081 ggactaactt cgcaagcatt gaatagcatg gaaaagttgc atcattaata aataagttgg
     1141 cattgagaaa attacaagcg cgtaatcata caggtctgtc ttaagggagt cttcgaaccc
     1201 tgatgttgta gtatgtcaaa acatttagtc aatcatatag gtgacttgat ttaactttat
     1261 ctgatagtct gattgtaatg attgtactaa ttgaatggag gcgtatgtaa ttgaatctga
     1321 gtaaacaaat taaaaagtat cgggaacgag atggttattc acaagaatat cttgctgaaa
     1381 agttatatgt atctaggcag agtatttcta attgggaaaa tgacaaaagc ttaccagaca
     1441 tacataactt attaatgatg tgtgatttgt tcaatgtaac tttagatgat ttagtaaaag
     1501 ggaccattcc atttgcacct gatattaaag cgcaacgaag tcttaactta tggacatatg
     1561 tgatgcttat tttcatgaca ctagctgcaa ttttaacagg acctttagtt gtttattgga
     1621 attgggcttg gggtataacg gtgatagtca tttatggaat aggttattat gcatctatga
     1681 aaatagaaga ctttaaaaaa gtgcataaaa tggataacta cgatcgaatt gttgctttta
     1741 tgaatggaaa agatcctagt gaagtacaaa cgactaaagc tagaaatata aaaacaggtg
     1801 tactttcttt catagcattt gttggtgcaa tcattcttat agctttgatt agtatgcatc
     1861 tggcaaataa gtttttataa atcatcgtgg tatggcctca tattatttat attatctaaa
     1921 atagcataaa aaaataccaa caagatttag aaccttgttg gtaatcaaag cgattcattt
     1981 ataatgagtc gttttatttt gtaagattaa acagtttgta cgttaactgc ttggtctcca
     2041 cgttgacctt cagtgatttc gaaagtaact ttttgacctt cttctaaagt tttgtagcca
     2101 tcgctagcga tacctgagaa atgtacgaat acgtctccgc cattttcttg ttcgatgaaa
     2161 ccaaaacctt tttctgcgtt aaaccatttt actgtaccgt tattcatata gaaaacctcc
     2221 gtgtgctttt gcacttaata tttgtaacaa attcataact aaaaaagagg atattctaaa
     2281 caaatacact acaatttaat tcacgagctt ttattacgta agtccaacta tacgctcata
     2341 ttggcataat gtacagtgtt ttttgaaaat aaatttaaaa agatttttaa aaaccttaga
     2401 aacgttgatt taaaggggtt tataagcatt tgaaaattta tcaattatat tgatatatcg
     2461 gtctaaatac tgataccatt tttatctttt gtaaaatgat tgacgttgta tatcttatag
     2521 agctctccaa aatatatatc tgttaattta tcaaaaaaat attgacaaat tggtgaatat
     2581 aagaaaatat aaataatata catatatatg tataaattca agttaatgag tttttatctt
     2641 tctaaacagt cttattgcag aaagagttaa ctaaaaatta taggtgtcat atgagtcgta
     2701 atttaataca tttatccata ggggtcgcta tatttataat ttctttctcg ttttatttat
     2761 taaatcaacc aaataattgg gcatggagca gtctaaatat atgtataaat gattcaaagc
     2821 catccaatgt aaaagttgct attttagata cgggtattaa taatacaaga ataaacaata
     2881 attttgttac aaaaagatac aatgctataa acaatcataa tcaaataact acagatagta
     2941 atcacgccac tcaagtagca agtattatca catatggaga acataaagaa aagttgattg
     3001 gtatcagtaa atcagtaagt atttatgacg ttaaagtatt agatgataac cttaaaggcg
     3061 atatagacaa cataataaaa ggcataactt ggagtatcaa aaatgacgta aatattattc
     3121 atatgagttt tggctttcag cgttacaata aagaattaca tgatgctata aagaacgcta
     3181 aaaataaagg tattatctta gtagccgcag cagggaataa catggatgat tatagtgact
     3241 atccagcacg ttttaacgaa gtatattcta tcacagcaat agacaaaaac aataagccat
     3301 ttatatatgc acctactaaa aaaatcgatt ttaaagctcc tggcgtagac gtatatacaa
     3361 aaggatcaaa agatgagtta cttactgtaa atggaacatc ttttgcagcc gcctatttca
     3421 ctgcttatct gacaaataat cttgaattcc gaaatgactt accacatatt ttaaaagaat
     3481 atccaataaa ataacgcatc aaaggagaaa acatgaaaaa caagataatt atcctatttc
     3541 tagcaattat tataactttt acttacggaa actttgcaga agcttctgtg gttataaaag
     3601 aagctaatat taacaattcg aatagtaaaa taaacacaaa taaaattaac gagaatattt
     3661 acagtaaaaa taatcgtaat tacattgaca ataactttaa atctaaagat ttaaacattg
     3721 aaagcagttt agaacacgac ctaaatacag ataaaattac tgtagatgct tcattaaaag
     3781 ataattataa taataaactt gataaaactt atgatgttaa atttttaagg attgttaatg
     3841 aaaatgactt taaggcagaa tttaccgacc aagatacggg agaaaaaata atatatgata
     3901 cgaatgaggt taaagcttca attgcacctg tagtggctgt attagtagga ttcatcgcca
     3961 aacaaggact taagaaagct attcaaaaat atggtaagac agttgtaaca tcaatgattc
     4021 gtacaagccc tcaagttgcc gcgcaggcag ctaaaaaatt gggttattct gctacaaagc
     4081 atgtatccca tggcaaaaaa gtgttcaaga aaaatagtaa agggcgtcct caatatatat
     4141 ctgtcgataa agatggacac cgaggaggag catggaaagg tgcaagcagt attaagaatt
     4201 taggtagcaa gaaaacaaga agtggtacat acgatgctaa cctaaaaaga attggagatt
     4261 aaaatgatta ttaaccttga aacacaacaa atacattttg atttagaaca tgaaattccc
     4321 tattttcaat cacctgagaa aaataggata cgcttagata ttgatgttct caataaaaag
     4381 caaatttcta atattattaa tgttatattt aataaccaat caaagacgaa atgtacattt
     4441 ttgtctgaat acttatatcc tgttaaattt agagaaaaaa caagaattgg tcgttttttt
     4501 aatattacta actggtacga agaaatccat tctgccgatg agaagtatgt tattgcaact
     4561 attaaaaata taaaaaacaa agacttaatt gattattctt tatatattaa aaaaggttta
     4621 gtggagccat ttattgtatt ctataatcaa gaaaatatat tctacattag caatgctgtt
     4681 atagatatca tcagtgtatc caaagatact cttggttata taaagtcaga attcaatgat
     4741 cactgtgctt cgccatactg attttatagc acagagaaaa gccgtagttt cgaagagacc
     4801 aacggctttt ttataaatct ttagattgtc aaattaagct ggacagttta tcatctttga
     4861 tgaaactcat aaatacttcc aatggtgttc tataacctaa agattttcta ggacgatgat
     4921 tgattttttg agcaacactg atgatatgat catccgttac taaattgaaa tccagttcct
     4981 tatctaatcc actgcgtcta agtattccgt tagaatgttc atttaaacct cgctgtgctg
     5041 gcatgcttgg atcagcaaaa aaatatagat atatcgtgac agttacatat tgttttccag
     5101 tttgagaact ccttaccaca atcaaaagta attgatttaa atatatgaga aggtaaagca
     5161 gaaaacattg agttcagagc attttctata tcagttgcct tacgacctaa ggatcgtaag
     5221 gtgataatca tcttggatac tctttctacc aaagtaatga ttgcactacc atgacgacca
     5281 acaatagtat ctccttcgag atgtccaaac tccttattga ataaagggaa ctcttctatt
     5341 ctttcagaaa tatgtctttt aaaagtctat ccacctcttt tttcagaatg gccatttggc
     5401 tttcttttac atttcatagg taaatcatgt acagcaaaaa tgttctcttt aaacattctg
     5461 taaagtgttt taacagaaca attgatcgtg tgttcacctc tgccaataat agtatcaggt
     5521 gtctatccat cagctacgtt ctctcggata taatctgact gttcttctgg aagcactatt
     5581 ctatgtctcc cacaattaga ctttctttgt ttgtattctt gataatattc aagggcagac
     5641 atacccgact taaaagcatt gattacgtta taaattgtct gtctagaacg tttcaatcta
     5701 ctcgaaatct ctttaactga aagattctag tgataatatg agtctatgaa cgtaagttca
     5761 tccgtgttaa ggtgtgtata ggccattctg tactaactcc ttatgttttc tttggtagga
     5821 aatacatatt gagtttagca cagaaggctt atttaccttt ttcttcgcgg cgttttaaat
     5881 aataagcgcc acctaataaa ccagctggaa tacctataat tggtgttgta acggtgctta
     5941 agacaataac aataattgct aaagcaataa cgttatacca agttacagtc aagtttttta
     6001 aatcctcgta tgattgtttt actaattctc taaatttcat gattcaatct ctccttttta
     6061 taaatcagtt aatagttata ctgcattgct taaaatcaaa atctcaatga atcatctttt
     6121 aatcacaagt gttaaatggt tttcttaatc aatttatttg aatgtacaaa agcgattaaa
     6181 gcaaaaacaa tgtaaatgag tgtgtataca atgattacta taataaccgg catgaaggat
     6241 atattaccca tcaatttcat aaatgctatt gcggcgaata ctgcatgtaa tatagcaatc
     6301 aataatggta aaccgaagtt gaaagtaatt tttagtaata atcctttaag catatctgtg
     6361 tgcgtaaagc ctatgcgttt taatattctg aagttactta gttcatcctc agtttcatcc
     6421 atttgtttaa tataaataat acatccagct gctactaaaa atgctaatcc taaaaatgaa
     6481 gtaacgaata ttagaatacc gttagtagca tcgacctctt ttttcatgtc atcataagtg
     6541 atgattttgt ctccaaactg ttttgcaatt gcttgagctt ttttcttttg tgatgtttgt
     6601 ttaatatcat atccataaaa agtatgaacg ttattttgtg ttttcaattg ctgatacttt
     6661 tctggactta cttcaatgac aggtgaatta aagattagat ttaaaggata aaccttgtct
     6721 ttgtcttctt gtgtaacacg aaaagtctcg ttctttgtac cttttactac taaatcattg
     6781 tttaaatgaa tattaatcac gttaggaagt gattttgtat ttgtaatgat ggcattgtta
     6841 ccagttaact ttgtatttgc acttaaaata gaattcgtgc gacctgaatc actaccattt
     6901 tccaaagtaa taacctgatc tttaacatta tctacagtaa tagtttcata ataattttta
     6961 gaaaatgtaa tatgttgttg gcttagttta gattcaaatt gttgcgcatc ttgagaggcg
     7021 accacgttaa aatcattggg tgccatagat gtaagggttt gatcggtatt tgatttagat
     7081 aatgctgcga aacacaatac agttactgta actgcagaaa taattgcaat gatagttaaa
     7141 gacatggcat ttttcttcat tctgtacata atagaagatg taaatacaac atctgtaata
     7201 gatacgcgtc catttttaga ttttttcaaa gttttaaaaa ttagtgacac ggaacttctg
     7261 aagaataaat aggcacctac aaccgttaaa aataaaatga taaacggtga tgtcatagcc
     7321 atagttagtg ctttgaacgt accaaacatt tctgtcgcca tataatatcc tagtgcaatc
     7381 atagcaatac ctaatacgcc tgaaatgact tctataactg taactttcgc agttgtaaca
     7441 tcagtcttaa ctgagtcttt catcattgat aagatactgc gtctttttaa aaataaactg
     7501 ctttgaaata aaatcagtat atacgcaata attagcatta aaatagttaa aacaagggcc
     7561 ataggttcga aatgtatcga taagttaatc gataatgaca tcaatttaga tacaatggaa
     7621 agcaataatt gtgcacctgc aatgccacat aatacaccga caacacctgt gattataaat
     7681 acaatcattt gttcaagtgc taacattttc aaaatgtttt gtcgtgttaa accaatcaac
     7741 tgaaatagcg caaattcacg tgtacgacgt ttcacgaata aatgattggc atacattaaa
     7801 aagatgacaa taatgataaa taaaaatatt gatccgacta aagcaccttt cttaatgata
     7861 gccatcgagt cgtcattatt tacaccttta gtaaactgta aggttgtaaa actgaaatat
     7921 aagacgatgc taaaaaataa tgaaaataaa tacattgcat aatgttttaa gttttgacgt
     7981 aagtttttta aaacgatatg gttaaatgtc atttgagaca ccacctaata ctgattgaag
     8041 atgtacaatg tcttcataaa aggcctgttt agaacgtcct tcctgataaa gttgtgtatg
     8101 aatttggcca tcttttagca tgatgacacg ttctgcaaaa cttgctgcaa ccggatcatg
     8161 tgtaaccatg acaattgtgg tatcaaacga tttattcatt tcttccaaac gttgtaataa
     8221 atcatttgca cttttcgagt cgagtgcgcc tgttggctca tctgcaaata tgatttgtgg
     8281 tttgtgaaca aatgctctcg ctgctgcagt tctttgttgt tgaccaccag ataattcgct
     8341 agggtattta tttcctaggt cataaatacc taatgctgtt gtgattgctt tataattttc
     8401 ttccattgtt gctttcgaca ttttttgaac agataaaggt aacataatgt tttctttaac
     8461 agttaatgtc ggcagaatac tgtaatcttg gaagatgaaa cctaatgatt ctttacggaa
     8521 tttggcaagt gctttttgat taagtttatt aagctcttgt ccgttagcaa tcacgctacc
     8581 gctagaaatt tggtcaattg aacttagtac atttaataag gttgtcttac ctgatccaga
     8641 aggccccata atcgcaacga attcgccttt ttgtatgtca aagttaatat ctttaagtgc
     8701 ttgaaatgtg tgctttttac cgtatgtttt tgaaacatgt tgcactgata atatcgtcat
     8761 agtatcactc cttttgtatt taaattcatt ttaaataata agtagaacag tagcctttat
     8821 ctaaacttac aattcaatgt ttgaatctta catagttgaa agatattaat atatcgtgat
     8881 tttctcgggt gaatttggat tattggaaag cagttcaact aatttgagca tcacagtatt
     8941 tgcactgagg aaatgtttct ataagttaag atgagggaat ttaaacaatg aataatagtg
     9001 acactaatta aaatataaag aaggcgttgt ttgaaaggaa atgattgtta tggaaaaagg
     9061 aaatcaagga attaaatggt ctagtttgat tatgggtgtg ttgttattaa tgttagcagt
     9121 tgttattttt acattcccaa ttgaaaactt ttatgcaatc acatggttaa ttggattatt
     9181 tgtattgatt aatggtgtga ttcaaattgt ttatcgtaga aaagccaaag ctttagttgg
     9241 tggtaaccaa aattggattc tgtttatggg gattgtagat attctatttg gtcttattgt
     9301 catttttaat gttggtgcta gctcggcatt ttttatctac atgtttgcat tttggtttat
     9361 ttttagttct attgctggat tatttacgtt ttcaggtagt ggtagcttaa aactaatttc
     9421 agtgattttt aatttattag gtattgtttt cggtgtcatt ttattattta atccattaat
     9481 gggtatcgtc tttatttcga cgatgattgc tattgcattt gtattcgtag gtgtcattta
     9541 tgttgtagat gcacttgctt aagtaaaatg aagcggttca aaagaagggt gtggcatgaa
     9601 gtttgtgcca tatccttttt gttgtgtttg cacagagtgt caataaagaa atactttttc
     9661 tttagaaatt agtatttttt atgcatgcgt tttacgcatg aatgtctatt tataagtaca
     9721 cacgagctct ggctaatgtg tgagaaccac taaatcatta attgttagtg gcgctgtatc
     9781 attttttccc acatcctaac gaacatgtgt attaaaaaag gacgttacct acattaaagt
     9841 aagttacgtc cgtattttta tgattaattg tcgctgtttt caagtcgact aattgttaca
     9901 tttaatccaa aatattttgt taaaaattgt ttatagttat cttttgtgat agctaacttt
     9961 tctggcgttc catcttttgt taaagttaaa tggttttcaa acattgtagc acgtccaaat
    10021 gattgtggca ttgtaattaa taaatgtttt acaaatactg aatcaggatg tgtttgatta
    10081 tattcaatgt tttgatcgaa ttcttctatt tctctagcct tgaattcagc ttcgtatttt
    10141 gtatgccaat gattattttc atatttttga acatagaaaa tgtccttatc ctcactgttg
    10201 aaaatagcgc ggaatgtccc actgatatca gtgatctgct gtgtatcatc tggtaaagta
    10261 atcggtatgg catttagagg taagtctcca aagccgacat cggttacata aaagacatta
    10321 ttaatagaaa caacaagtga tgcatgtgaa ccgttttgac tacgaccgcc accgggtgta
    10381 tgaatggtag ccgacattaa ttctgggtta aatccttttt gttgtaaata tgctttgaaa
    10441 aatgtgttta actcataaca aaaaccaccg cgtttatcat gaacaatttt attgaaaagt
    10501 gcatcgatat ctacagatat tggtttacca ttttgaacat caatattttc gaaaggcacc
    10561 gttaacataa agcgagtcgc ataataattt aatgcttcaa tactcggttg attataacga
    10621 gttggttcaa tttgtagata atcttctaac tttgcaatat tcattagtat atcgcctcct
    10681 gtattaaaga ctataagaga attgtaaaca gaaatgctaa aattttaaat aataaagcac
    10741 tggattttac aagatctatt ttagatagaa aaatacgctc tcaaaatgag gtcatctcta
    10801 aaagaaacga tttaaagatg actactgaga gcgtattcta aaggagtcag tatgcagggt
    10861 gtctaaaaac gcaataataa aaagatgatt gcaagacaag ttactttatt tagaccattt
    10921 gtgttctatg cgaccaaact tccaaattaa actcgaaata agccaagtaa ttaaaaataa
    10981 agcgactaga atatagccta aataatcaaa ttcgattgaa ctaataaatg cccaaaatgc
    11041 accgtgtaag tctagcttat cagcaagaat ttgcagcaat tcaatcatcc caatcactaa
    11101 tgcagctatg acggatatcg cagtaatcgt tatattgtaa tagattttgc gaataggatt
    11161 gaagaatgcc caattatagg catatttcat tacaacacca tctaaagtat ccaacaaact
    11221 catacctgat gcgaataaaa ttggtaaaga taagattccg ataaatgaaa tggcctgttg
    11281 tgacgcgcct gaagaaagag cgagtaacgc aatttcacta gctgtatcaa aaccaagtcc
    11341 aaataaaaag ccaagtggca atacgtgcca actacgcgtg attaatttga aataaggtcc
    11401 tacaaatcga gaaacaaatc ctctagattc aagtaatgca tcgacttcag cttcttcaat
    11461 gtgttcacga cgtaatttag cgaacaagtt aattaaagag attaaaataa ttagattcaa
    11521 cacaccgata agcactaaaa agaaacctga tactagtgta ccaatcgttc caccaatatc
    11581 ttggaaatgt ggtaattcat ctttagccca ttttacagat acccctaaaa aaacagccat
    11641 taaaaataca acagatgaat gtccaattga aaagtagaaa ccaacaccag atggatcttt
    11701 gcgttgctgt aataatttgc gaaccgtatt atctatcgcg gcgatatgat cagcatcaaa
    11761 tgcatgacgt aaacctaatg tatatgcaag aatccccata ccaaataaga tatgatggtc
    11821 ttttccagca atccataaaa aactaaaccc aataacgtgt aacaaaatga caatagctat
    11881 gtatggtaac cagcttaagc gctcattttt aaaaacagtc aaaatatatc cttctttcat
    11941 aacatgatat ttaatcatac tgtatgttca atgtgcactc tagtaataag tgttcatata
    12001 acaaaaatgt tatgcaaaat tatttgttat ataaaaatat acatgtaaaa gcaaaagaat
    12061 tttttgcgat atataatttg agtcattaac caacaacaat gtaagatgtc actttgctta
    12121 acttggcatc ctttttatga ttttcaaatt caaaaaaatg agcaaaatga atctctttac
    12181 cagtttttaa tatttgacta ccgtgcatgg aaccgaaata gccatgtgtg atgatggatt
    12241 gaatattgag cgaagcaatc tctttataat gattagatag ttcttgaatt aatatttgag
    12301 gtccataaat gtcaaagtgt ccaggcacag accaaataaa ttctgttgta accagtgaac
    12361 gtaataattc aatatctaat gctgctgtag caactataaa atctatcatt tgttgacgtt
    12421 taggggcatg attgcatggc acgtctcctg ttaataaaaa aggtaatgat gaatgaactt
    12481 ccgttttaaa atgtagttgg tgctgaaata aagcttgttc caatattgat atagctttag
    12541 gaccaacgcc atgcatctcc attagagatg actttgtata ttgtgataca tcttctaatg
    12601 tgtatatacc ttgtgaattt aatgcacgtg ttgcaggctt tccaatttta ggtaaagtca
    12661 tttgtatcca tcctttatgt tgtttggtag tctttctggc aatataccct gatttgtaca
    12721 gaattattta taaaattaat attgtttagt ggttggcgaa taaatgtgga aaatataaac
    12781 aaaatgatat ctttatcgaa atattattag taaaatcgtt atattagctt aactaagtga
    12841 gcgtaacatg ttaaaaattt gggagagtaa ggaggtatgt aagtgaatac agaatataaa
    12901 aaaggtatat ttttagcact tagtgcttac attctgtggg gaatactacc tatatattgg
    12961 cagttcgttg atgcaatagg cgcatttgaa attttagcct ttcgtattat attttcagta
    13021 atattcatga ttttcatact cgcggttgga caaaaacaac gcaatgcatt tcaacgagat
    13081 atgaatcaat tgttagccaa gcccattcaa ttactagcaa ttgtcgtagc gggttatgtt
    13141 atcacattaa attggggtac atttatttgg gctgtaacga acggtcacgt actacaaaca
    13201 agtttaggtt attatataaa tccacttgtt agcattttgc tcgcacttat ctttttaaaa
    13261 gaaagattca ataaatttga atggctagcc attttattcg cattcatcgg tgtattatat
    13321 atgacgctca agattggaga attcccaatc gtctctatta tattagcgtt atcctttggt
    13381 acatacggat tattaaaaaa agtagtacat attgatgcca tcagcagtat tacgattgaa
    13441 tgtattgtta ccgcacctgc tggactcata tacgttattt atttatggca gcaacatcag
    13501 atgtcatttg gattgaacat gtcatcattt tggttattat tttctggtgc tattacggca
    13561 ataccactaa tcctattctc agccggggca aaacgtattc cactttcgct aataggattt
    13621 attcaatacg ttggaccaac aataatgttt gtactcggca tatttgtttt caaagagcct
    13681 tttagtatag atcaattaat tacgtttata tttatttgga caggtattgt gttatatagt
    13741 ctttctcaat acattaaatt gaagaaacat ccggtcgcaa aaaccctata aaatgcgagg
    13801 agagataatt atacatgttt tcaacttcct ttgtgctatg ttaaggtgag actgaggtat
    13861 gtgtgcagta atagtgacag ccgtataaat ttgaaacatt aaatataaaa taaagcactt
    13921 tcgttgaatt cataattaaa tgaatttaag cgaaagtgtt ttttgtttga tgatttatga
    13981 tacataagta atgttaatca atgaagcgga ttaatataga aatgtatttt atgtgatgtt
    14041 acgtaatgac ttaaatcttt atacaaattt tcagtgaagt acgcaatgat ttaaaggata
    14101 aacgtatttt aaaaagggga gtcttgattt agcaaagatt gattttaaat acaaattaaa
    14161 aacaggctgg gacataaatc aatgttctat gctctacga
//
LOCUS       DAMZPP010000074        14188 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMZPP010000074 DAMZPP010000000
VERSION     DAMZPP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14188
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.074"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            89..1210
                     /locus_tag="POH73_001652"
     CDS             89..1210
                     /locus_tag="POH73_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDI6887599.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPIKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            1259..2527
                     /gene="icd"
                     /locus_tag="POH73_001653"
     CDS             1259..2527
                     /gene="icd"
                     /locus_tag="POH73_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDI6887600.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALTEFGDQVFTWQQYDEIVEKEGRDAANVAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            2917..3618
                     /locus_tag="POH73_001654"
     CDS             2917..3618
                     /locus_tag="POH73_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6887601.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVEN
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKE"
     gene            3621..5282
                     /locus_tag="POH73_001655"
     CDS             3621..5282
                     /locus_tag="POH73_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDI6887602.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKEIVKQQNIDLLITKNDKKVFSSGKLKPTKTIDNYSQKKDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTEIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
     gene            5783..7270
                     /locus_tag="POH73_001656"
     CDS             5783..7270
                     /locus_tag="POH73_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887603.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLSFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQVIILSMIAALIIYLLLR
                     SDRYLHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFANIIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLISICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVYTV
                     ESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
     gene            7563..10193
                     /gene="polA"
                     /locus_tag="POH73_001657"
     CDS             7563..10193
                     /gene="polA"
                     /locus_tag="POH73_001657"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDI6887604.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASGEDAIEKTFEIETSFDNVDFTSLKEATIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDIINYAELVSWLENPNTKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIYTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGFVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            10209..11081
                     /gene="mutM"
                     /locus_tag="POH73_001658"
     CDS             10209..11081
                     /gene="mutM"
                     /locus_tag="POH73_001658"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDI6887605.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFTNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKHGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            11097..11720
                     /locus_tag="POH73_001659"
     CDS             11097..11720
                     /locus_tag="POH73_001659"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDI6887606.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEGD"
     gene            11890..12915
                     /gene="gap"
                     /locus_tag="POH73_001660"
     CDS             11890..12915
                     /gene="gap"
                     /locus_tag="POH73_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDI6887607.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDDQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPHSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            13125..13595
                     /gene="nrdR"
                     /locus_tag="POH73_001661"
     CDS             13125..13595
                     /gene="nrdR"
                     /locus_tag="POH73_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDI6887608.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            13596..>14188
                     /locus_tag="POH73_001662"
     CDS             13596..>14188
                     /locus_tag="POH73_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase DnaB"
                     /protein_id="HDI6887609.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHSRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESF"
BASE COUNT         5154 a         1873 c         2645 g         4516 t
ORIGIN      
        1 agtttttata tttcacaaac gaaatgcgtt tagctataat attagttgaa agccatttca
       61 taaagaaaca gtaaagggga aatttatcat ggcagaatta caaagaggtt tagaaggggt
      121 tatcgcagcg gagactaaaa taagttcaat tattgaaagt caattgactt atgccggcta
      181 tgatattgat gatctagctg aaaatgcgca atttgaagaa gttattttcc tattatggaa
      241 ctatagattg ccaaatgaag aagagcttgc tcatttaaag gggaaattaa atcaatacat
      301 gacattaaat cctcgtgtgt atacacattt tgaggagtat gttacagatc acgtgcatcc
      361 aatgacagca ttacgtacgt cattatcata tattgcacat ttcgatcctg atgctgaaaa
      421 tgaatcagat gaaaatcgtt atgaaagagc aatgcgtata caggctaaag tagcatcatt
      481 agttacagcg tttgctcgag taagacaaga taaagaacca attaagccta atcctgactt
      541 aagttatgca gcaaacttcc tatatatgtt acgtggggaa ttaccaacag atatagaagt
      601 ggaagccttc aataaagcgc ttattttaca cgctgatcat gagttgaacg catctgcatt
      661 tacagcacgt tgtgcggtat cttcattgtc agatatgtac tcaggtattg tagcagctgt
      721 aggttctctg aaagggccat tacatggtgg tgcaaatgaa caagttatga cgatgttatc
      781 tgagattggg tcaattgaaa atgttgatgc ttacttagat gaaaaatttg ctaataaaga
      841 taaagtaatg ggcttcggtc atcgtgtata taaagatggt gatcctagag cgaaatattt
      901 aagagaaatg agccgtcaaa ttacgaaaga cgctggtcgt gaagaattat ttgaaatgtc
      961 agtgaaaatg gaaaaacgca tggcagaaga aaaaggatta attccaaatg ttgattttta
     1021 tagtgcgagt gtttatcact gtatggaaat acctcatgac ttattcacgc caatctttgc
     1081 tgtaagtcgt tctgcaggat ggattgctca tattttagaa caatataaag ataatagaat
     1141 tatgcgtcct agagcaaaat atattggcga aacgaatcgt aagtatattc cgcttgaaga
     1201 aagaaaataa tcaatacaaa ttaaaaatga agatgtaaaa tttggaggta aaataactat
     1261 gactgcagaa aaaattactc aaggaactga aggattaaac gtacctaatg agccaattat
     1321 cccatttatt atcggtgatg gaattggacc ggatatttgg aaggcagcaa gccgagttat
     1381 agatgctgct gttgagaaag cctataatgg cgaaaaacgc attgaatgga aagaagtgct
     1441 agctggtcaa aaagcatttg atacaactgg cgaatggtta cctcaagaaa cacttgatac
     1501 aattaaagaa tatttaattg ctgttaaagg acctttaaca acaccaattg gtggtggtat
     1561 tagatcatta aatgtagctt tacgccaaga attagattta tttacttgtt taagaccggt
     1621 acgttggttt aaaggagtac catcacctgt taaacgtcca caagatgttg atatggttat
     1681 tttccgtgaa aatactgaag acatttatgc tggtattgaa tttaaagaag gaacaacaga
     1741 agttaaaaag gtaattgatt tcttacaaaa cgaaatgggt gcgacaaata ttcgattccc
     1801 agaaacttca ggtattggta ttaagccagt ttctaaagaa ggtactgagc gattagttag
     1861 agcagcaatt caatatgcta tcgataataa ccgtaaatca gttactttag ttcataaagg
     1921 taatattatg aaatttacag aaggctcatt taagcagtgg ggttacgatt tagcattaac
     1981 tgaatttggt gatcaagtat ttacttggca acaatatgac gaaattgttg aaaaagaagg
     2041 cagagatgct gctaatgttg cacaagaaaa agctgaaaaa gaaggcaaga ttatcattaa
     2101 agattctatt gctgacattt tcttacaaca aattttaact cgtccagctg agcatgatgt
     2161 tgtagcaact atgaacttga atggtgacta catttcagat gcattagctg cacaagttgg
     2221 tggtattggt attgcgccag gtgcaaacat taattatgaa acaggtcatg ctatttttga
     2281 agcaacacat ggtacagctc caaaatatgc aggtttaaat aaagtgaatc catcttcagt
     2341 aattttaagt tctgtattaa tgttagaaca tttaggatgg caagaagcgg cagataagat
     2401 tacagattca attgaagata caattgcttc aaaagttgtt acttatgact ttgcccgttt
     2461 aatggatggt gctgaagaag tttctacatc agcatttgca gatgaattga ttaaaaattt
     2521 aaaataagca aaataaactt aggtgagtct agcaacttta attgctaggt tcaccttttt
     2581 aaatgaattg aagttggcag caggatagaa atgataaaga gtcacgaatg attgattatg
     2641 tagtgtttct tacacattaa ctaaagcgaa tgtgtatata agcttaggaa taaatgagta
     2701 aaactcatgc ataggcagtg ggatagaaat gatattttca caaaatttat ttcgttgtcc
     2761 cagagtatgt catgcaaatt atagaaggaa ataaaagaag tattttattt atgtgtaaag
     2821 taatgtaaaa aatgtgtaaa gataatgtgt aggatgttat ttttataaat agttcggtta
     2881 taatgtaaat ataattataa gttagggagg cataccatgt cgcaaaaagt gttggtagta
     2941 gatgacgaac attcaatcgt aacattactt aaatataact tagaaacagc tggttatgaa
     3001 gttgttgtcg catttgatgg tgatgaggct ttagagaagg tagaaaatga acagccagat
     3061 ttaattattt tagatgttat gctacctaaa aaagatggca ttgacgtatg taagactgta
     3121 agaacaaata aaaatttagt ccctatatta atgttaacgg cgaaaaatga tgaatttgat
     3181 cgggtattag gtttagaatt aggtgccgat gactatatga ctaagccttt ttcacctaga
     3241 gaagttgttg cccgtgtgaa agccatttta agacgttctc aatttgtaaa agaagtagaa
     3301 gagattgacg atgatattac catcggatcg atacgtatta gacccgatta ttttgaagta
     3361 tataagcata atgagctatt ggagctaaca ccgaaagaat ttgaactgtt gctttatttg
     3421 atagaaagac aaggcagagt cattacgaga gagcatatgt taaatacggt atggaattat
     3481 gaatttgcag gagactcaag aattgttgat gtacatatca gtcatttaag agataaatta
     3541 gaagataatc ctaagaagcc acaacttatt aaaactgtgc gtggcttagg ctataaattg
     3601 gaaagaccta aagaataatg atgaagtttc accaccgctt aatgttattg ataagcagta
     3661 tattaattat cagttttgtt acattaggca ttattattca cgcttccatc acatatgttt
     3721 tatcagaaaa acacgaacaa gctctagcaa cagatgcaag atcatatatt tatttagtcc
     3781 aagataatga gattgataaa gttaaagaaa ttgtaaaaca gcaaaatatt gatttattga
     3841 taactaaaaa tgataaaaag gtgtttagta gtggcaaact gaaacctact aaaacaattg
     3901 ataattacag tcaaaaaaag gacgttttta tttttaataa gaaggttaac ggttatcatg
     3961 tatggattaa aggttacaat aacgatatta cagaaatgca ttggacgttg tggaaatatc
     4021 ttatattgac atgtttagtt gtacttattt gtctgtattt tgcaagccgt agttttaagc
     4081 gtactttaat ccgtcccatt caagaagtta cttatgcaac acaattgttg gcaaatggtt
     4141 attatcacat acgtgtacct gaaagtaatg ttgtagaaac taaagcgctt tttgtgtcta
     4201 ccaatgattt agcacgacgc cttcaaaagc ttaataatga gcaaaaaatt caatccaacc
     4261 gtttaaaaac aacgattgaa aatattccaa gtgctatttt aatgatagat cgaaatggaa
     4321 aaatcgttgt tgcaaataaa gcgtattatg aacagttcaa catctcgcat aatattgaac
     4381 aagtaggcta tcacggctat gttaatactg aaattgagca attaatttta gaaagtttta
     4441 aagttgaaaa acctatctat gaacaattgg aagttgcaat aaaccaagtc catgccaagt
     4501 actttgatat ttcttgtgtt ccgattttaa ctagatctca aaaaagtttg caagggattt
     4561 tagttgtgat gcatgacata acaaatctga aacaacttga aaatctaaga cgtgaatttg
     4621 tagcaaatgt ttcacatgaa ttaaaaacac ctattacttc aattaagggg tttgctgaaa
     4681 cacttattga tggtgcaaaa aatgatgcgg aatcattaga catgtttttg aacataattt
     4741 taaaagaatc aaatcgaata gaatctttag taacagatct gttagattta tcgcatattg
     4801 agcaacatac cgagttagat acagactata tgaatttatc agatttaacg cggcgtatta
     4861 ttgataatat gatgactcaa gccaatcaaa aaaatatttc cattcatact gaaattgaaa
     4921 aagatgtcat tgttaaagca caagaaagta aaattgctca agttatcacg aatttgttaa
     4981 cgaatgcaat taactattct tatgaagatg gagatattaa cgttcgtgtg tatcgagatg
     5041 actttcgtgt cattttcgaa gtacaagatt ttggtatagg tattaaatta gaagaccaac
     5101 aacgtatttt tgaaagattt tatagagtag ataaagcacg aagtagagat tctggtggga
     5161 caggtcttgg attatcaatt acaaagcata tagtagaggc ccaccaaggc aatattgaag
     5221 tgaatagtca agttggcaaa ggctcaacgt tcaaagttat tctaaaagat tataaagaat
     5281 aaaaattaaa agtggtaaca gcgcgtgtat ttttcacgaa gttgttgctg cttttttatt
     5341 tcgttcaaat ctctattggt attaaattag agttaatgaa gtggaatata ttcggttgtc
     5401 aattttgtca tttgcatatg tcgttttgta agttataagt aatagattgt tgtgaatcaa
     5461 gcaattttat aatatattta ttataataaa gaaattttag taacacgctt aaaatagatg
     5521 cttaattaaa aagtaataag ttttatttga acggaaataa atcttaattt aaacacttga
     5581 atacacaagt gtaatcattc gtcatagaaa aagacgaggg acgatttaga taaaatgttg
     5641 aaaagtaata ttaaatacct tgtttatcta atttgattga ggaaacatgc ataggtgaaa
     5701 attagaaata cattaaaaat attgaattaa tttttcgacc tacaagttga ggaaaattta
     5761 actagatttg aggattagca aagtgaaaac aattcaatta cacaagacaa ctttgatatt
     5821 aattgcgata ttattgtttt atacttttat gggtatctta ctaccactca tgcacgatga
     5881 tttacaatgg tttagcaatt ataatactga tattttaaaa gtaggatttg catcacttaa
     5941 tggtcgctat atcggaaata tctttgaaat tatagctgta catgtaagct ggttacgttg
     6001 gctttcatat ggtctcatta gtatgggtat catttggatg attatgcaca ttacacgttg
     6061 taaagcgtgg acaagctatt atttgttagc attttctttg atgttaattt taccaagtgc
     6121 tatttatgca gatacatatg gctggtttgc aggattttat aattatgcga catcaacact
     6181 aatttcactc tttatcattt attattgtat taatgcaatt atatataaag aaaagcaacc
     6241 ggtaagtgtt acagtactat tctatgtttt aagcttcttc ggtcaattat ttatggagaa
     6301 tgtgacatta ttccactgta tcattttaat tttagctttt ctgtatgaat ttactgcaaa
     6361 tcgaacactt aattataaat tattgttttc atttatgatt gctactatag gtacaatcat
     6421 tatgttttcc aatccaaatt atcgcaaaat tttatttgaa ggatcagaat atcaacaagt
     6481 ttctaataat caaggtatct tttcaaaatt tgcagaaatg atatcgacat cacttccata
     6541 tggtgtcatt ttcagtcagg ttataatttt aagtatgatt gcagcgctta ttatttatct
     6601 actgcttaga agtgatcggt atttacattt aacaatatta aagcgtcgta tcattatgat
     6661 aggatttatc actttaccgc tttattattt actattttac aatcagtttt tattaaataa
     6721 aaatacagat attggattgg ttggttttgc gaacataatt gtatgtggtt actttgcgtt
     6781 ttcgatattt gtaggtattt atttgtctat taatgatcgt aaaacacaaa ttactttata
     6841 ttccttactt atttcaatat gtgtttcggc gtcgacacta gtcattgtta ctccaattga
     6901 accaggtaat tttttgattg tttatacgat acatgtaatc attttaatta ttttattaaa
     6961 agaatttcgg aaatataagt caatcaatgt taattttatt aaaggtgcat cactagtttt
     7021 agctgtgata tatcttagtg catttattta tgttcattat gaacatgaaa aaagagttca
     7081 gttattaaaa caacaaatta aagatcatcc acgacaagag gtttatacag tggaatcatt
     7141 gccgtttgaa cattatatgc atcatccgtc accaatcaat aaaaattacc aagagtggtt
     7201 taataattat gaagaattac cgaaaatgac caaagttaaa tatattccat ttggttctga
     7261 cgattcataa cttattctta cctataaaaa tttataatag cagcataaaa atattaaaaa
     7321 gtagacataa tcatgaaatg ataatgtgca tctcaaaagt tggactttta acctgacttt
     7381 attgagtgct catcaagaac aatcatgatt atgtcttttt attttttagc ttagtctata
     7441 tagtcacttg ttttatttga aattaggtcg tgtataaata ctctatgaga tagtttcttt
     7501 aattacgaca tcacagtcga atatgctaaa atatatataa attagttctg gaggttaaaa
     7561 ttgtgaataa attagtatta atcgatggta atagcttaag ttttagagct ttttatgcat
     7621 tacctttgtt atcaaacaaa gcaggcattc ataccaatgc agtatatggt tttgcaatgt
     7681 tactagagaa gattttaaaa gaagaaaagc caaatcattt tttagtagcg tttgatgcag
     7741 gtaaaacgac tttcagacat gaaaaatata gtgaatataa aggtggacgc cagaaaacgc
     7801 cgcctgaact aagtgagcaa tttccttata tacgccaatt attagatgct tatcatatta
     7861 aacgttatga attagataac tatgaggcag atgatattat cggaacttta agtaaagaag
     7921 cagataaagc gggctttcag acaattatta ttacgggaga ccgagattta acacaacttg
     7981 caacggataa tgtaaccatt tattacacta aaaaaggtgt tactgatgtt gatcattata
     8041 cacctgactt tattgctgaa aaatacaacg ggttaacacc taatcaaatt attgatatga
     8101 agggattaat gggagatact tctgataata taccaggtgt tgcaggtgtt ggagagaaaa
     8161 cagcaataaa attgctgaac caatttgaca cagtagaagg tgtttatgaa catttagatg
     8221 aaatttcagg taaaaagtta aaagaaaaac ttcaaaacag caaagaagat gcattaatga
     8281 gtaaagaatt agcaacgatt aatgtagata gtccgattga agtaaaactt gaagatacgt
     8341 taatgactca tcaagatgaa caacaagaaa aaatcgaatt gtttaagaag ttagaattca
     8401 aacaattgtt ggctgacatt gatcaatcag caagtggtga agatgcaata gaaaagacat
     8461 ttgaaattga aacgtctttt gataatgttg attttacttc attgaaagaa gcgaccatcc
     8521 atttcgaatt agacggtggc aactatttgc gaaataatat tttaaaattc tctttattta
     8581 caggtgagaa acatattgta attaatgcgg atgacataat taattatgct gaactagttt
     8641 catggttaga aaatccgaat acgaaaaaag tcgtatatga tgctaaaaaa acatatgtag
     8701 catcacatag attgggaatt gatattcaaa atatttcatt cgatattatg ttggccagtt
     8761 atataattga tccatctcgt acgattagtg atgttcaatc agttgtttca ttgtacggtc
     8821 aaagttttgt gaaagacgat gtgagtatat atggaaaagg taagaaattt aaggtacctg
     8881 aagatgatgt tttaaatcca tatgttgctt ctattactga tgcaatttac tttgcgaaac
     8941 caaatatgga taaacaatta gaagaataca atcaggtaga actcttagct gatttagagc
     9001 taccgctagc taagatttta agtgaaatgg aagaaattgg tatatataca gatgttcatg
     9061 atttagaaga aatggaaaaa gaaattcaag aaaaattaga cgtcttgatt cgaaatatcc
     9121 atgatgcagc tggtgaagat tttaatataa attctcctaa gcaattaggt gttgtattgt
     9181 ttgagacatt acaattacct gttattaaaa agacgaaaac aggatattct actgctgtag
     9241 atgtcttaga gcaattgcaa ggtgaacatc ctatcattga ttatatttta gaatatcgtc
     9301 aattatctaa gttgcagtct acttatgttg aaggattgca aaaggtaatt agtgatgatc
     9361 aacgtatcca tacacgtttt aaccaaactt tagcgcaaac tggacgttta tcaagtgtag
     9421 atcctaattt acaaaatatt ccggttagac ttgaagaagg gcgtaaaata agaaaagcct
     9481 ttaaaccaac ttcaaaagat agcgttatat tatcagcaga ttattctcaa attgaattgc
     9541 gtgtattagc acacattaca caagatgaga gtatgaaaga agcatttatc aacggcgatg
     9601 atattcatac agcaactgct atgaaagtat ttggtgtaga agctgatcaa gtcgatagtt
     9661 taatgcgtcg tcaagcaaaa gcggttaact ttggaattgt ttatgggata agtgattatg
     9721 gtttaagtca aagtttaggt attactcgta aaaaagcaaa agcattcatt gatgattatt
     9781 tagctagttt cccaggtgta aaacaatata tgtctgatat tgtaaaagat gccaaagctt
     9841 taggtttcgt ggaaacattg ctacatcgtc gacgctatat tcctgatatt acgagtcgta
     9901 actttaattt acgtggcttt gctgaacgta ctgctatgaa tacgccaata caaggcagtg
     9961 ctgcagatat cattaaactg gcaatggtta aatttgcgca aaaaatgaaa gagacaacat
    10021 atcaagctaa actattatta caagtacacg atgaattaat ttttgaagta cctaagtcag
    10081 aagtagattc atttagtgaa tttgtagaag agataatgga aaatgcattg caattagatg
    10141 ttccattaaa agtagactca agttatggtg caacttggta tgatgcaaaa taaagtgagg
    10201 ttaatggtgt gcccgaatta ccagaagtag aacatgtaaa aagaggaatt gaaccctacg
    10261 ttataaatca gaaaattgaa cacgttatat tttcggataa agtaatcgaa ggtaaagctc
    10321 aaggcaagga aacaattata aaaggaattg aattagatac ttttaaaact ttatcggaag
    10381 gctataccat tactaatgta gaacgaagaa gtaaatatat cgtttttcaa ctagataata
    10441 aacgagaaca acgtacatta attagccatt taggtatggc gggtggtttt ttcattgtag
    10501 atgaacttga agatattatg atacctaatt atcgtaagca ttggcatgtt atttttgagc
    10561 tgtcaaatga caaaaagtta atttattcag atatacgtcg ttttggagaa attagaaatg
    10621 ttgcgtctgt agcatcttat ccttcatttc tggaaatagc gcctgaacct tttacaaatg
    10681 aagcattgac gtattattta aatcgaattc atcaacagtc taataagaat aaaccaatca
    10741 aacaagtgat tttggatcat aaagtaatcg caggttgcgg aaatatttac gcatgtgaag
    10801 cgttgtttcg tgcaggtgtt ttaccagata aaaaagtaaa agacttaaca caccaacaac
    10861 aagaaatggt tttttattat gttagagaag tactagaaga aggtattaag catggcggta
    10921 caagtatttc agattatcgt catgctgatg gaaaaactgg tgagatgcaa ttgcacctca
    10981 atgtatataa gcagccggta tgcaaagtgt gtggaagtca aattgaaaca aaaatcatcg
    11041 cgacaagaaa tagtcattat tgtccagtat gtcaaaaata aagaaagagt gaaattatgc
    11101 cgaaagttat tggtctaaca ggtggaatcg cctcaggaaa atcaacagta tcagaactct
    11161 tatccgtatt cggttttaaa gtagtagatg ctgataaagc agccagggaa gctgttaaaa
    11221 aggggagtaa aggtttagct caagtacgag aagtctttgg tgatgaagca attgatgaaa
    11281 atggtgagat gaatcgtcgt tatatgggtg atttagtgtt taaccatcca gaaaaacgct
    11341 tagaattaaa tgctatcata catcctatcg tgcgagatat tatggaagaa gaaaagcaag
    11401 aatatttaaa acaaggatat aatgtaatca tggatattcc attattattt gaaaatgaat
    11461 tggaaaatac agtagacgaa gtgtgggttg tatacacttc tgaaagtata caaatggatc
    11521 gtttaatgca acgtaataat ttgtcattag aagatgcaaa agctcgtgtc tatagccaaa
    11581 tttctattga taaaaaaagc cgaatggccg atcatgttat cgataattta ggggataaac
    11641 ttgaattaaa acaaaacctt gagagattgt tagaagaaga aggttatatt gaaaagccga
    11701 attacggaga aggagattaa tattactcta taaataagtc attactttac gtatgcgtgg
    11761 atgtatgtaa gtaatgacta ttttttataa aaaagataaa taaatcaacg gaaaacgctt
    11821 tcaaatttca tataatatgc tatactaatt ccataaagta taacacataa aaatcaaggg
    11881 gtgcttttaa tgtcaacgaa tattgcaatt aatggtatgg gtagaattgg aagaatggta
    11941 ttacgtattg cattacaaaa taaaaattta aatgtagtag cgataaatgc tagttatcca
    12001 cccgaaacaa ttgcacattt aatcaattac gatacaacac atggaaaata taatctaaaa
    12061 gtagaaccga ttgaaaatgg attgcaagtt ggagatcaca aaattaaatt ggttgctgat
    12121 cgcaatcctg aaaacttgcc atggaaagaa ttagatatcg atattgctat agatgcaact
    12181 ggtaaattta atcatggtga taaagccatc gcacatatta aagcaggtgc caaaaaagta
    12241 ttgttaactg gtccttcaaa aggtggacat gttcaaatgg tagttaaagg cgtaaatgat
    12301 gaccaattag atatagaagc atttgacatt tttagtaatg cttcatgtac tactaattgc
    12361 attggtccag ttgcaaaagt tttaaataat cagtttggaa tagttaatgg tttaatgact
    12421 actgttcacg ctattacaaa tgaccaaaaa aatattgata atccacataa agacttaaga
    12481 cgtgcacgct catgtaatga aagcattatt cctacttcta ctggtgcggc gaaagcttta
    12541 aaagaagtat taccagaatt agaaggtaaa ttacacggca tggcattacg tgtaccaaca
    12601 aagaatgtat cgctcgttga tttagttgtt gatttagaaa aagaagtaac tgcagaagaa
    12661 gtaaaccaag cttttgaaaa tgcaggttta gaaggtatca ttgaagtcga acatcaacca
    12721 ctagtgtctg ttgattttaa tactaatcca cattcagcta ttattgatgc aaaatcaaca
    12781 atggttatgt cggggaataa agtaaaagtt attgcttggt atgataatga atggggttat
    12841 tcaaatagag ttgtagatgt cgctgagcaa attggagcac ttttgacatc aaaagaaact
    12901 gtaagtgcaa gttaatacaa ttaatttata gcctaactct gacctcggag ttaggttttt
    12961 tatttttaat ataataatat tgtcatgctt attacttaat aagtaacgat ataatgtttt
    13021 ttattgtttc attatttggt taaatgaatt aaattgaggg attctttttt catctctaga
    13081 tttatagtat aattaggcat aatcaaaata aagagggtga tgtaatgaaa tgcccgaaat
    13141 gtaattctac acaatctaaa gttgtagatt caaggcatgc cgatgaatta aatgccattc
    13201 gaagacgaag agaatgtgaa aattgtggaa cacgtttcac tacatttgaa catatcgaag
    13261 ttagtcagct tatagttgtg aaaaaagatg gcacaagaga gcagttttca agagaaaaaa
    13321 tacttaatgg acttgtgcgt tcttgtgaga aacgaccagt tagatatcaa caacttgaag
    13381 acataacaaa caaggttgaa tggcaattac gagatgaagg tcatacggaa gtgtcttcac
    13441 gagatatagg tgaacacgtt atgaacttgt taatgcatgt tgatcaagtt tcatatgtta
    13501 gatttgcatc agtctataaa gaatttaaag atgttgatca attgttagca tcgatgcaag
    13561 ggattttaag cgaaaacaaa cggagtgatg cttaaatggg acgacaagcc tttgaattcg
    13621 gcttaagacc aaaggatcaa ttcaaagtta tgcaacactt tgatttaaat acgaatcatt
    13681 tagaggtatt gaatcgactg tatacaccgc taattggaac acaagcagtg ggtttatacc
    13741 attttatgac tcaattcgtt aaagaaagtc ataatgaaac attgattctt tctcattata
    13801 tttttatgaa tgaacttaaa attaatttac tagagtttag acaacaaatg gacttacttg
    13861 aagcgattgg attgttaaag gcgtttgtca aacatgatga acaagaaaca caatttgttt
    13921 accaattaat tcaaccgcct tcagcacatt tattttttaa tgatccaatg ttgtcaatct
    13981 ttttatatag tgaagtcgag catagtcgat ttcatgagtt gaagaaatac tttgagtacc
    14041 aacaaattga tttgagtgaa tttaaacagg taacgagaca atttactgat gtttttaaag
    14101 tgccgtctac aaaaatagat atcgatacaa gtgatattcc gattaatgaa ccgtaccaag
    14161 gaatcgattt atcaaatgaa tcttttga
//
LOCUS       DAMZPP010000075        14155 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMZPP010000075 DAMZPP010000000
VERSION     DAMZPP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14155
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.075"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..509)
                     /locus_tag="POH73_001663"
     CDS             complement(<1..509)
                     /locus_tag="POH73_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887610.1"
                     /translation="MDNSFLESLKEILEPYDDIESNKDGFVFGGYIDWPSFVDYEDFQ
                     VEEVMSLLGLDIPFGSSKNPFEFYEILKVIQNIEKGLYEAICDEAIIIKEDEEISTED
                     FIDSIMDYKVQIPTVDRLKSSITELFEKYEIPQGIQYEYDEGMPESSKYWSLEMDEDL
                     YFIKEINFN"
     gene            complement(697..1826)
                     /locus_tag="POH73_001664"
     CDS             complement(join(697..1580,1580..1826))
                     /locus_tag="POH73_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558209.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDI6887611.1"
                     /translation="MTRERRLFSSEFKLQMVRLYENGKPRNEIIREYDLTPSPLGKWI
                     KQYQNTGTFNHQDNLSDEEKELIKLRKEVQHLKMENDIFKASSVDHGTKIEVIQKNAH
                     QYSVSAMGNVLKILRSTYYDSIKRKDNKITKDDSNVERAVINIFNANRKVFATRRIKN
                     HLNDKGHTVSRRKIGRIMKKYNLVSVYTKAKYKNHPKETNEKLIKNHLNRAFNREQPM
                     ETLVSDLTYVKVAGTWHYICLFIDLFNREIVGYSAGKNKDANLVSKAISRINHNLEQI
                     KLFHTDRGKEFDNHLIDEVLETFKIKRSLSTKGCPYDNAVAEATMKAMKTEFVKQMQF
                     ENLEQLKTELFDYVNWYNNFRPHSSLQYLTPVAFKNLHMKTV"
     gene            complement(2075..4195)
                     /locus_tag="POH73_001665"
     CDS             complement(2075..4195)
                     /locus_tag="POH73_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6887612.1"
                     /translation="MLELITDFSKKSFKNYSMKEELKFNAINIIYGVNGRGKTSLARG
                     IKEIIEENNPDSLRYFYTDYIHELLLLEDSNKFKGVKATFGTKNVEIENKIIKLKNEV
                     VDMTDTKKLLVEKRRKLRELINEIHKSRKGNLKIPLKSSNKSIEEVIAIYEKNLKDAK
                     KIEHNIESIRNFVANDETLYNKYNSIYEVMIPSLKIETYDVDRLTKILQNNYTDIKIP
                     SFEVIDWLRNGLELHNVEESICKFCGNNLNYNLIKEKIEIYLLNEKKKDFDYLKDVEK
                     SIEQLSDNYEQYLSNIDIFVNELGVQKSVEESLGDSIDEIKRILKQKLGNMENDNLKF
                     PSDDYITLISRLSEIEEECKEKKKIKLKELNKKTENINVIVTGSISLEILENQSIREE
                     LSSIQYEENECKKQEQLNEEINAKISKLHENQSDYNDFKIYLNGVFESINLHIRLKSD
                     ETTQNYYLYHDLENISLNIKDISEGEKNLIAFLFFYFELFEDEKQETIKSNIKTLIID
                     DPINSFDEANRFYVLELIKKVLKSKFNQIFIFTHSWNDFCDITYRLKGEDHNFYEVYK
                     DHQGTSFLEKFKKVKTPYKKLFQEIYELSRKSQKDIVEEDCYYYHSINSIRRVFEEFL
                     SFKLKNSDLAQKSNQPEIEEVYRKMTGNEMSNNKKIKLGSFLTIINVLSHKPYRAIDV
                     IGSAKFLMRYIEDVDKAHYDAMKD"
     gene            complement(4315..4794)
                     /gene="rlmH"
                     /locus_tag="POH73_001666"
     CDS             complement(4315..4794)
                     /gene="rlmH"
                     /locus_tag="POH73_001666"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDI6887613.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(5162..7480)
                     /gene="adsA"
                     /locus_tag="POH73_001667"
     CDS             complement(5162..7480)
                     /gene="adsA"
                     /locus_tag="POH73_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HDI6887614.1"
                     /translation="MKALLLKTSVWLVLLFSAMGLWQVSSAAEQHTPMKAHAVTTIDK
                     ATTDKQQVPPTKEAAHHYGEEAATNVSASAQGTADDTNNKVTSNAPSNKPSTAVSTTV
                     NETRDVDTQQASTQKPTRTATFKLSNAKTASLSPRMFATNVPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDMNKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQSSKGSESGKDAQPIGKDKVMDPA
                     KQPAPSKVVLLPAHRGTVSSGREGSDRALEGTAVSSKSGKQLASMSAPKGSTHEKQLP
                     KTGTDQSSSPAAMFVLVAGIGLIATVRRRKAS"
     gene            complement(7707..8507)
                     /locus_tag="POH73_001668"
     CDS             complement(7707..8507)
                     /locus_tag="POH73_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6887615.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAGHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(8895..9683)
                     /locus_tag="POH73_001669"
     CDS             complement(8895..9683)
                     /locus_tag="POH73_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDI6887616.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPASILNKSVKGIQLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGSEYQLSEISSGSVKYEQTYDNFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNSPKIINH"
     gene            complement(9684..11018)
                     /locus_tag="POH73_001670"
     CDS             complement(9684..11018)
                     /locus_tag="POH73_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887617.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLSSDFTLFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALATVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGANQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNPDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(11011..12837)
                     /gene="walK"
                     /locus_tag="POH73_001671"
     CDS             complement(11011..12837)
                     /gene="walK"
                     /locus_tag="POH73_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDI6887618.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSTKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(12850..13551)
                     /locus_tag="POH73_001672"
     CDS             complement(12850..13551)
                     /locus_tag="POH73_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6887619.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
BASE COUNT         4040 a         2661 c         1950 g         5504 t
ORIGIN      
        1 tcattaaaat ttatttcttt aataaaataa aggtcttcat ccatttctag tgaccagtat
       61 ttagatgatt ctggcattcc ttcatcatac tcgtattgta taccttgagg aatttcgtac
      121 ttttcaaata attctgttat tgaactcttc aacctatcca ctgtagggat ttgaacctta
      181 taatccataa ttgaatctat aaaatcctct gtactaattt cttcatcttc ctttataata
      241 attgcttcat cacaaatagc ttcgtataat cctttttcaa tattttgtat aacctttaaa
      301 atttcataaa attcaaacgg atttttagaa ctcccaaatg gtatgtccaa ccctaataaa
      361 ctcattactt cttcaacttg gaaatcttcg taatcaacga aactaggcca atctatatat
      421 ccaccaaaca caaaaccatc tttatttgat tctatatcat catatggttc taaaatctct
      481 tttaggcttt ctaaaaagct attgtccatt atttatctcc ttttaaaata tcctcaatta
      541 ttggtttaat tccaatagat ataatatctt taattaatct ttgagcatga tatctgttaa
      601 cagaatatgc tatctcattg agaataatcg ttgcaatttc ttttactcta aatgaaaagc
      661 tatccttcaa gatttcattg gtatgtcaac atttttttag acagttttca tgtgtagatt
      721 tttaaatgcc actggcgtca aatactgtaa tgaagaatgt ggtctaaaat tgttgtacca
      781 atttacataa tcaaataatt ctgtttttaa ctgttctaag ttttcaaatt gcatttgttt
      841 tacaaattcg gttttcattg ctttcatcgt tgcttccgca actgcgttat cataaggaca
      901 acctttggta cttaatgaac gtttgatttt aaatgtttct aggacttcat ctatcaaatg
      961 attatcaaat tctttgcctc tatcagtgtg gaatagtttg atttgttcaa gattatgatt
     1021 tattctgctg attgcttttg atactaaatt tgcgtcctta tttttacctg cactgtaacc
     1081 aacaatttct ctattaaata aatctataaa taaacatatg taatgccatg ttcctgcgac
     1141 ttttacatat gtcaaatcac ttactaatgt ctccattggt tgttctctat taaaagcgcg
     1201 attcaaatga tttttaatta gtttttcatt agtttctttt ggatgatttt tgtatttagc
     1261 tttcgtataa acagaaacta gattatattt tttcatgata cgacctatct ttcgtcttga
     1321 tacagtatga cccttgtcat ttaaatgatt tttaattcgt cttgtagcaa agacttttct
     1381 attagcatta aaaatattta tgacggcacg ttctacgttt gaatcatctt tagtgatttt
     1441 attatctttt ctttttatag aatcataata ggtacttctt agtattttta agacgttacc
     1501 cattgctgat actgaatatt gatgtgcatt cttttgaatg acttctattt tcgtcccatg
     1561 atcaacgctg cttgctttaa aatatcgttc tccattttta aatgttgaac ttctttacgt
     1621 aatttaatca gctctttttc ttcatctgat aagttatctt ggtgattgaa tgtacccgtg
     1681 ttttgatatt gctttatcca ttttcctaac ggcgaaggtg ttaaatcata ctcgcgtata
     1741 atttcattcc taggcttacc attttcatat aatctaacca tttgtaactt aaactctgaa
     1801 ctaaataatc ttctttctct tgtcataatc aaatgaaatt ccacaggtat ttacgcttct
     1861 gtcgaagctt ataaataatt cattattcta cctctatttg gagctaatct tctcactgta
     1921 gtaaacatag gtgataagtt actacaatct gcttcgtgta tcaataagtt tctatttctt
     1981 ctaaatttta ttttcagcat acttataata gttcaattta tgttctccat gaattttaag
     2041 ttcttttcaa ataaaaaatt atacgaccat taattcaatc cttcatagca tcataatgag
     2101 ctttatctac atcttcaata tacctcatca aaaattttgc tgaaccaatt acatctattg
     2161 ctctgtatgg tttatgtgac aatacattta taattgttaa aaatgatcct aactttattt
     2221 ttttgttatt actcatttca ttgcctgtca tttttctata aacttcttca atttctggtt
     2281 ggtttgattt ctgagctagg tcactatttt ttaacttaaa acttaaaaat tcttcaaaca
     2341 ctcttctaat gctatttatt gaatgatagt aataacaatc ttcctcaact atgtcctttt
     2401 gactttttct cgataactcg taaatctctt gaaataactt tttataaggc gtctttactt
     2461 tcttaaattt ttctaaaaaa ctagttccct gatgatcttt gtacacttca taaaaattat
     2521 gatcttcccc ttttaatcta taagttatat cacaaaagtc attccaagaa tgggtaaata
     2581 taaatatttg attaaatttg cttttaagta cttttttaat taattctaaa acgtaaaatc
     2641 tatttgcttc gtcgaagcta ttaataggat catcaatgat taacgtttta atgttacttt
     2701 taatggtttc ttgtttctca tcctcaaaaa gttcaaaata aaagaataaa aatgctatta
     2761 gatttttctc tccttcactt atatctttta tatttagtga tatattttct aaatcatgat
     2821 aaagataata attttgtgta gtttcatcag atttcaacct aatatgtaaa ttaatacttt
     2881 caaatacacc atttaagtaa attttaaaat cattataatc cgattgattt tcatgtaatt
     2941 tcgatatttt tgcattaatt tcctcattta gttgttcttg cttcttacat tcattttctt
     3001 catattgaat acttgaaagt tcttctctta tactttgatt ctctaatatt tctaaactta
     3061 tagagccagt aactattaca tttatatttt ctgttttttt atttaattct tttaatttta
     3121 ttttcttctt ttccttacat tcctcttcaa tttcagataa tctgcttatt aatgtaatgt
     3181 aatcatcaga aggaaattta agattgtcat tctccatatt tcctaatttt tgttttaaaa
     3241 ttctcttaat ttcatcaatt gagtctccca aactttcttc tactgacttt tggaccccta
     3301 attcattaac aaatatatca atattagata aatattgttc ataattatcc gataattgtt
     3361 caattgattt ttcgacatct ttaagataat caaaatcttt tttcttttca tttaataaat
     3421 atatttctat tttttcttta atcaaattat aatttaaatt gtttccacaa aatttacata
     3481 tactctcttc aacattatgc aattctaaac cattacgcaa ccaatctata acttcaaaac
     3541 taggaatttt tatatctgta taattatttt gtaaaatctt tgttaatcta tctacatcat
     3601 aagtttcaat ttttaggctt ggaatcataa cttcataaat gctattatat ttattataaa
     3661 gtgtttcatc attcgctaca aaatttctta tactttctat attatgctct atttttttag
     3721 catcttttaa atttttttca taaatagcta ttacttcttc tatactttta tttgatgatt
     3781 ttaatggtat tttaagattc ccttttcttg acttatgtat ttcgtttatt agttctctta
     3841 acttccttct cttctcaact agtaactttt tagtatccgt catatcaaca acttcatttt
     3901 tcagcttgat tattttattt tcaatttcta cattttttgt accaaatgtc gcttttaccc
     3961 ctttaaattt attggaatct tctagtaaaa gaagttcatg aatataatca gtgtaaaaat
     4021 acctcaagct atctggatta ttttcttcta ttatttcttt aatacctcta gctaatgaag
     4081 tttttcccct cccattaact ccgtatatta tatttatagc attgaatttt aattcttcct
     4141 tcatactgta atttttaaaa gacttttttg aaaaatcagt aattaattcc aacattatat
     4201 tccccttttg tataatatat tcacatcacc tttatgatgt tatattttat ttttttgctg
     4261 tttttatcac catattgaat ttatactata ttaatagtta ctccattttt agttttattt
     4321 gtgatacgcc tctccacgca taatcttaaa tgctctgtac acttgttcaa ttaacacaac
     4381 ccgcatcatt tgatgtggga atgtcatttt gctgaatgat agtgcgtagt tactgcgttg
     4441 taagacgtcc ttgtgcaggc cgtttgatcc gccaatgacg aatacaaagt cgctttgccc
     4501 ttgggtcatg cgttggttca attcttgggc caatccttcg gaagatagca tctttccttg
     4561 tatttctaat gtaatgactg ttgattgtgg tttgattttg gctagtattc gttggccttc
     4621 tttttctttt acttgctcaa tttctttgtc gctcatattt tctggtgctt tttcgtctgg
     4681 aacttctatg atgtctatct tggtgtatgg gcctaaacgt ttttcatatt ctgctatggc
     4741 ttgcttccaa tatttctctt ttagtttccc tacagctaaa atggtgattt tcatgtcgtt
     4801 tggtcctcca aattgttatc aactttccag ttatccacaa gttattaact tgttcacact
     4861 gttccctctt attataccaa tattctttgc agtttttgat atttttctga cattcatgcc
     4921 caatttttag tgtataacct gtgtatttgt ggataactct gtgggcattg tgggtatttc
     4981 tctttaaatt gtacacaatt ctggataact atccccattt ctgtggataa ataccttgat
     5041 gtcttattac ttatccccgt ttttatatcc catatttatc aacggttata gctttttttc
     5101 ataaaaaaac actaccgtac ctcttgttta agaaatacag tagtattgtt ttcaatatat
     5161 tttagctagc ttttctacgt cgtacagtcg caattaaacc tatacctgct actaatacaa
     5221 acatcgctgc tgggcttgaa ctttgatcag ttccagtttt tggtaactgc ttctcatgtg
     5281 tgctaccttt aggcgctgac atgctagcca attgtttccc gctcttactt gatacagcag
     5341 ttccttccaa tgcgcgatca gaaccttctc taccactact aacagttcct ctatgcgctg
     5401 gcaacaatac aactttactt ggcgctggtt gtttcgctgg atccatcact ttgtctttac
     5461 caattggttg tgcatcttta ccagactcac tacctttgct actttgttgt cctttagctg
     5521 gttgttcact tactgctggt ttacctaata acatacgttg tggttctgtc gtatcatact
     5581 tagctaagtt agctgttttt aaataacttg ctagtacttg atctaatgaa ataccttctt
     5641 ctctagggcc accgaacata ctatatccgt cgccacctga tgctgtgaag tcattcatcg
     5701 ttacgtgata tacacgtttt aaatcaatat tttcaaactt acctgtctct ttatttaaaa
     5761 tttgaatagc attaattcgt ttgccagacg gtttattcat atcatagtaa acacggattg
     5821 aatcagagat atgtagtaaa ccgccattcg ctgttaatac tgtcttacca tctttttgtg
     5881 ttgttggtgc gcctaaacta tgttcaaaag ccgtccagac gtctgaacct tttacatcaa
     5941 tttgcgcaat cgtatttcca aatggtaata ctgaaattaa atcatagcgt gtcaccttac
     6001 cttttgcgat agaggcacga atacctccac catttgtcac ggcaaagtca gtctttttag
     6061 agaaattctt aacgccatat gcttccatag catctgcaat cgcgtttcct aaatttgttt
     6121 cacgcgttct aacgtcatct ctttctcctt tgaaatcaat cgtattattt ggaataataa
     6181 cctctgctgt ttgtgctcta aatgtttgat cagcttgatt aatttgttca gctaatgctt
     6241 tgttcggtgt tacattttca acgtctttaa cattaatcaa tgatggttta atatttgata
     6301 cctctccatt gcggtaatta aatgtaatct taccgatatt cgcaagtgct gtacctgttt
     6361 gtgctaatgc atcattgtta taaatttgac cattttgaag tacggtatgt gaatgaccat
     6421 caatgactgt aatacgtttc ttcaattgtg gattttgact taattgtttc actaagtaat
     6481 cgccacgcca tgtttcttgt gttgaaggat caatacctaa atgagatata acaacaaatg
     6541 tatctacgtc tttataaata cgcatcattt ctgctgtcac actttgtaat ggatctctaa
     6601 attcaacacc tttaatgcct tcaggccttg ttttcgtctt tgtttctggt gtcgttacgc
     6661 caataattcc ataacgaata ccatttttcg ttacaatcgt tgaaggttta aatgcgcgtt
     6721 ttccatcttt ataaacgtta gtacttaaca tcgggaagtc taacataccc tctaactttt
     6781 tcaactggtc gtatccaaag tcaaattcgt ggttaccgac tgccatagca tcataaccta
     6841 ctgcattcat cgctttagcc atttcttcac ctttagactg gtttgaaagt ggtaaacctt
     6901 ggaaggcgtc tcctgcgtct aacattaaat caggcttttc ttgttctttt actgttttta
     6961 atttagccat accgatgaca cgcccttttt cttcggctag tcggccatgg atatcatttg
     7021 tatgtaatat tttatgtgtt gttgtttgtg gtacattggt agcaaacatt cgtggtgaaa
     7081 gtgatgctgt tttagcattt gataatttga atgttgctgt gcgagttggt ttttgtgttg
     7141 aggcttgttg tgtatctaca tcgcgcgttt cgtttactgt tgttgaaact gctgtagatg
     7201 gtttgttaga tggtgcgttg gatgttactt tgttgtttgt atcatcagct gttccctgtg
     7261 ctgatgctga tacgttggtt gccgcttctt caccataatg atgagccgct tcctttgttg
     7321 gcggtacttg ttgcttatct gttgttgctt tgtctatcgt tgttactgca tgtgctttca
     7381 ttggtgtatg ttgctcagcc gcgctcgaga cttgccataa tcccattgca ctaaaaagca
     7441 aaacgagcca tacacttgtt ttaagtaata aagctttcat ttcgttccct ccattaactg
     7501 tcatacacca atatattatc ataatcctta tattaaaatg attgatttta tgtaaattat
     7561 ttataaatta catttaatat cgaattattt tttgcgtttc aactagaacc ttaatctcaa
     7621 caacagccat ttcccaccaa caacataaaa aaacacccga taaagtcgtt tcacagcagt
     7681 gcggaacttt atcgggtgac tctcatttat attgtatata ttggcgttgg aatagcttta
     7741 tccgtatcac atagcaatac ttctttttcc gtatcaatat cgtgttcgtt caatacttgg
     7801 ccaacactca tacgcgccaa atctttcatg ttattatctt gtgataaatg cgataggtaa
     7861 atacgtttcg tgttacctgt aatcacgtct gtcatcgcat gacccgcatc ctcattagat
     7921 acatgaccca tatcgcctaa aatacgttgt ttcgtcttcc atggataacg acacattctc
     7981 aacatatcga cgtcatgatt actttcaaaa ataaacgcat cgctgccacg tatcatacct
     8041 ttcatacgat cagacacgta acccgtatcc gttaaaatcg taaacttctt atagttatta
     8101 tggaaaatat aaaattgcgg atctatcgca tcatgtgaca cgttaaacga ttcaacatcg
     8161 aaacctgcaa tagatttcgt ttcataagga ttaaaaatga atttctgatc catagggatg
     8221 cgactatctt tcttttcaat tgcctgccaa gtcttttcat tcgcataaat tggcaattga
     8281 tatttacgcg ccaaaacacc taatccttta atatgatcaa tatgttcatg ggttactaaa
     8341 ataccattta aatcttgaat attacggtca atttgactaa acaattcttc cattttcttg
     8401 ccagtcaaac caacatcaac taatagacta cctttttcat tttctacaaa agtggcgtta
     8461 cctgtactac cacttgctaa tacactcatg cgtatcaagc ggctcatcct ttctatttaa
     8521 gcagtcaatc tctctatatg actgcaatat acatcagtgc aaatatacga ttttccctac
     8581 agaaactatc agtatattag ctgttttgta tacctttcct attctgtaac tttagttcat
     8641 cgaccggtct cttataatta aattttgata atcacgtgca atgaatgtcc taattattat
     8701 aacaatgatt agacaaatac taaagttttt aaaatagcag ttacgttaat tttacaatgc
     8761 ggttctactt aatatatgaa taaatgcgca tgttaggacg caaaaacgtc agccataata
     8821 aaccatacca gcaaaaagct agcaccgctt ctcacatatg atgagcttgc aatgctagct
     8881 tattacgatt catattaatg attaataatt ttagggctat tatttgtcgc ttcaacatag
     8941 taagtattcg ttttatcctt accgtcatgc ttcactttaa tttcccagtt tggttgtaac
     9001 aattgaacat tcgtttcgtt cacgactgag tagtaaccga gtctagcatt aatgacttca
     9061 tcaccttttt tcaagtaacg attataatat aatgcctcga ttgcttttct cacaccaatc
     9121 acttgatgct tcttatctgc accatctgtg ggcttaatgt catccatcat tgattgttta
     9181 taactagtcg ctttattatc ttctatatta aagttcaaca tcgctttact attattcaaa
     9241 atcgggaaat tatcatacgt ttgttcgtat tttacagaac cagaactaat ctcacttaat
     9301 tgatattctg aacctttgaa cacacgtttg ttaacataat ctttcaaatc ttttaagtta
     9361 ttgtcactga cctttaccga ttggctaatg tttgcattta ataattttcc accatctgat
     9421 gtggtcaaat ccgaatcgcc cttagcttta gaactaaagt cttttgaacg ccccgtaatt
     9481 tgttctaatt gtatgccttt aactgattta tttaaaatac tagccggtac tttgatttct
     9541 tcttgttgaa aattaacttc attgttactc tcaacttcat taatgtgtga gcgattgact
     9601 ttattaacat aaatcgatac taatacgatg ttgacaagaa taaacacgaa aatgaaaagt
     9661 gtcttcgtca gtttccagtt catttattca agcctcccat cgttataaac ataccattcg
     9721 ccatcatatt ctacatacca acgcggtacg agttcactgt taatctgcac ttcaatgtga
     9781 ttatgatccg gattatcttg catttcgtaa ccgatagcga tgtttgttac tttttcaaaa
     9841 ttaatatcac tattgttcgc taagcttgaa cgtacagact ctaatttcgg caacgattta
     9901 ttatcctcac tatttaacac aacatcggtg cgcagtaacg aacgacgata atcaaagacg
     9961 cccttttcac cccaagtgac ttgaatttga ttagcgcctt ctttattaaa cgttggataa
    10021 ccattaagga aacgttgata tgttagctcg cctgactgat tattcgtact aaacaatctg
    10081 aagtcttcgt ttaagaaacc accatgacca ttaataaaat caaaggttcc tggaatcgtt
    10141 tcttccattt tgcttgaact tgcttcatct tcggacaagt ttttataatg gtatttttca
    10201 tttttatcgt tatagtttgc gacacctgta ttattgttgt aagttgtaac accactcttt
    10261 gaactacgaa cgatggttga atcgtcaaat agtatagcat tcattttctc aacactaatc
    10321 gtgttaaata ccatgcgata tgtttttaac ttttcaggtt tacttggtgc aaaaacatgc
    10381 gtcgtacgat caattgtatc tttgtttgtg atgatatctg tgtatggttg catatctttt
    10441 ttcactgttg ctaatgcatc taaaaaatga tcatttttcg ttgtagttgt taattttacg
    10501 taatcgtggc gatctttgct tatagcataa agcacgatat tatcatcagc atcatgatct
    10561 atgaccaaac gattgaaatt gaaatgattt ggtactttcg cattcatgtt cagtacttgg
    10621 ccaagatatg ttgataacgg taaatcatac gtaaaatcga ataatgtaaa atcactgctc
    10681 aaatcaggaa tcatcaagtt atgatcacga cgaacatgtt ccacggactt cacttcttta
    10741 ttttttaaag gtttcgtcag cttatttaca ttagataccg tcgcaatcgt accttctgga
    10801 tgatcatttt tcgaatgaat aatctgaaat ggcgtaatag ttgtatccat tttggctgtc
    10861 ataggtgtcg ttaaaggttt cgtttcactc ttcttactat ctgtattgtc gacatttgca
    10921 atatcaggcg aaaagttcca taccatatat gtcaatacga cactcatcaa gacgagtagc
    10981 gctaaaatga cagatttaat atgttcctta ttattcatcc caatcaccgt cttcaatgac
    11041 ttcacatgga agtgtgataa agatagatgt gccttgacct tctacactgt ttgcccaaat
    11101 acgaccattg tgagcttcca cgatctcttt cgaaatggct agtcctaatc cagtaccacc
    11161 cattttacgc gtacgtgcct tatctacacg atagaatcgg tcgaatatct tatcgacttt
    11221 attgatagga ataccgatgc cattatcttt aatacgaatc gtcattcgat tataaagtgg
    11281 attttgtttc acgtggaact cgacacgttt atcgcctcta gaatatttca tcgcatttgt
    11341 aatgacatta tcaaatactt gcgtcatttt atcaggatca aattctgtga aaatcgtctt
    11401 tttcggaata tctcgaataa atgttgtatc tttcgtagac atttcatgtc gattaataat
    11461 tttattaatg aacatgttaa agtcgataat ttctttattg atttgatcag actcattatc
    11521 cattttagat aactgtagca agtcattgac cagtcgaatc attcgttctg tttcttcacg
    11581 ggtaacagat aaaaattgtg gcgcaagttc ctcatctttc catgcacctt cttcaagtgc
    11641 ttcaatgtaa ctattcatag aagttaaagg tgtacgtaac tcatgtgata cattggcaac
    11701 aaattcacga cgctcacgtt caacttgttg ttgttcagtc acgtcatgta acacagcgat
    11761 ataacccgtt acaaatcctg tttcctgcac aatcgtacta aagttaacac gtgcgattag
    11821 accttcttct tcatttaaat ctaataagaa actatcatta ttctcttgaa tttcttcaag
    11881 tttaaattca tcttcaagac ttaatacact taacatgtaa tatccgatga tgtcttcttt
    11941 cgccatacca agcatcttaa gtgccatatc attgacaata cgaatacgtc cacgacggtc
    12001 tgttgcaata ataccatcac tcatatgggt gataactgag tccagtctac gtttctcact
    12061 ttcagtatta gcctgcgctt cttgtacacg tttagacaag ttattaaatg ctaaagctaa
    12121 ttcgccaatt tcatcattac cataaatctt cacacgttgc gtatagttac ctctggacat
    12181 ttcaaccgtc tggttacgca tatcggtgat tggtttggta atcgttcgcg ctataaagaa
    12241 tcctaggatg actgtgatta ataatgaaat agctgtacca acaatgaata tttgatttat
    12301 attatttaat tggttataaa cgtcattaat ttttgattcg atataaatat taccaattac
    12361 ctttttatcg actttaacgg ggatattata tacccagaca cggtccttac caccgccata
    12421 atcttttaaa attaaatgat cgtttgattg tcctagtgat agtgcttttt ggacagaact
    12481 atcattcgct ttttgattga ttagactacg gttagactgc ttcgtcgtcg caataataat
    12541 ttggtcttta tctataaaac gaatttctcc aatttcttga cggttggcat actcacttaa
    12601 taaattttga atatcttttt gtgcatttac ggagcccttt tcgtcatata ctttttcaat
    12661 actaatttct aattgcttag cgtactgcgt aatattcttc ttaaaattat caagcagctc
    12721 tttttcaagg ttatttgtaa aatacagccc gataatttgc ataccaatga taatcagtaa
    12781 tacataaaca attacaagtt tagtatgaag ggattgtagt tgttttagcc acttcattcg
    12841 tttcgacctc tactcatgtt gttggaggaa atatccaacg cctctacgcg tcacaatata
    12901 ttcaggatgt gacggatcat cttcaatctt ttcacgtaaa cgacgaatcg ttacatcgac
    12961 tgtacgtaca tcgccaaagt aatcatagcc ccatactgtt tgtaataaat gttcacgtgt
    13021 cattacttgt cccatatgtt ttgacaaata atggaacaat tcaaattcac gatgtgttaa
    13081 ttcaatatct tcgccacgtt ttttaataga atatgcgtct ggataaatca caatatcttt
    13141 aattgtgatt tcattcgtta catttccagt gtcttgtgct ggttgtgagt aatgacgacg
    13201 taagttcgct ttcacacgtg cgattaattc acgcgtacta aacggtttcg ttacatagtc
    13261 atctgcacct agttctaaac caagcacttt atcaatttct gaatctttag cagtaagcat
    13321 tataattggc atttcgtatt ttttgcgcac ttcacgacat acttccatac catcacgacc
    13381 aggtaacatg atatctagta atacgatgtc tggttcttct tcataaatta agtcgactgc
    13441 atcattacca tcgtatgcac agtacacatc gtatccttct ttttttaagt taaattctaa
    13501 aatatcagca atcggttttt catcatcaac tacaacaact tttctagcca tttgcataaa
    13561 cctcttttct taaatcatta atattatttt atcatttttt gttctttatc gtttatgttt
    13621 gtgtaaaaaa tcacaggatg tcgcttacta tttttgttat tgtgcgcatt ttccgtctta
    13681 taacgtatag taacctatta tataatttac catttataag ctattaattc tattttccaa
    13741 tataaactta tatattaata ttttttatta atttctcacg gcggattatg ataatgatta
    13801 gtctttccct ataatatgac cacgaaatca tttttatatt attaaaggtg cgaaaaaatc
    13861 gggattccta ttggtgtgga tgtgaggaaa ataaatacta ttatatttat gttaagtgag
    13921 ctcttttggt ctatcactat gaaaaagtat gttaataaag gcacatggta atctaattaa
    13981 aaaatttgtt ttctatattt gtctttaata cattgcaact gaattaccag aagtttatac
    14041 attataaaga aaatggacga acagataaat caatatgatt atcattcgtc catatgtctt
    14101 ttattaatat aatattaaac gcaaaaaatg cctatcctct tggataggca tttgt
//
LOCUS       DAMZPP010000076        13856 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMZPP010000076 DAMZPP010000000
VERSION     DAMZPP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13856
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.076"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            72..446
                     /locus_tag="POH73_001673"
     CDS             72..446
                     /locus_tag="POH73_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887620.1"
                     /translation="MMLTIFVLSESYVSSLIVSNLPERDENLIDKAYEKIIQQYISKN
                     IRTRNGRKELVKNFYNTDLPAIPHTKLRDALAHEINKVDLKNNRFKYDNSRGNRNENI
                     IEVSIDEIINELEEWFLNLEFP"
     gene            736..2436
                     /locus_tag="POH73_001674"
     CDS             736..2436
                     /locus_tag="POH73_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6887621.1"
                     /translation="MKCIDKTWVSYYKELADKLTDYQNKRYELIEIVKEVYKKTGIKF
                     PTLASDNVLMDIDPFTIFALFNKNSMRETNKVKILTELASELNIKSKIPSVFDSIPTV
                     NNLNATYYNFKDYGKDNDIETLWTLFTAALNFANYPSEDNKLKFIHSFDKNITKTGNG
                     NSKITSGLYWIAPETFANFDSRSIWYIFDTYKLPKELINEQLKPKEKLTGRHYLELIN
                     MLKQVLKCQEVEINDFIDLSYESWHYSEMINEANRNKFDSNIKEIVVSENDNIIQNRS
                     RTTKVIPYGKQEFLDEVFIDESDYDRLVQLLRRKRNVILQGPPGVGKTFLAKRLAYSL
                     IGSKCKERIKLIQFHQSYSYEDFIMGYRPTSSGFELTTGTFYDFCKKADKDRDNEYFL
                     IIDEINRGNLSKIFGELFMLIESDKRDEELILLYSGERFSVPRNIYIVGMMNTADRSL
                     AILDYALRRRFAFYNMQPAFHTTKFKKLLKAINNTELESVINCIEGLNKEIVQDDMLG
                     NGFVIGHSYFSNIECIDKDELSNIIEFEIIPMLEEYWFDEPSKINVWSEKLRNSINND
                     "
     gene            2429..3469
                     /gene="mcrC"
                     /locus_tag="POH73_001675"
     CDS             2429..3469
                     /gene="mcrC"
                     /locus_tag="POH73_001675"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methylcytosine-specific restriction
                     endonuclease system specificity protein McrC"
                     /protein_id="HDI6887622.1"
                     /translation="MINIKNIYYMLSYAFTVLNKKGYQKLATEQFENIFDLYSAILIK
                     GISSQLNSGLHHEYIEQTDSLKVIRGKVDVKNSIQGLGVLSQRINCIYDEFSLNTYMN
                     KILKTTMKCLIKTDISRKNKIKLRKLLVHFNNVDTLDYRNIQWYHSFDRNNQTYKMLI
                     SICYLIFQGVIQTESKGQNDLMVFVDEQQISRLYEKFILEYYKKEFPELVVTSSNIQW
                     SLDNDDNVNMLPVMRSDIMLRYKDKCLIIDAKFYKNTLHNYYDTKKIHSTNLYQIFTY
                     VKNQQLNLKKKAIQVSGMLLYAKTDENIVLNDKFHMSGSQIIIKTLDLNCNFTIIKKQ
                     LNGIVNDIFLLK"
     gene            3900..4058
                     /locus_tag="POH73_001676"
     CDS             3900..4058
                     /locus_tag="POH73_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887623.1"
                     /translation="MITIDSKINKQIAKNLSLEGMYVTREQQIQILHAINNEKEITNE
                     LIRKIAFK"
     gene            complement(4366..4527)
                     /locus_tag="POH73_001677"
                     /pseudo
     CDS             complement(4366..4527)
                     /locus_tag="POH73_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095317.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(4897..5868)
                     /locus_tag="POH73_001678"
     CDS             complement(4897..5868)
                     /locus_tag="POH73_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDI6887624.1"
                     /translation="MTETKAIDTHAHLWNEDYLDKLGKLGSQGTEVAKGINQSASKED
                     LEKRFKMMDDAGVDLQIISATPQSPQWGTKEEAHQSAQEINDLYERLVQQYPDRFLAY
                     GAVSLPYVDQAIEEAQALLKKDEFVGIAIPTIVKDKVSVADKQFEPFFAAMNELNATI
                     YIHPTGCGAQSPLVNDYQLEWVIGAPLESTFITLQLIKNEIPQKYPNIKFHISHLGGA
                     LPFFMTRIKDNYEDWNAFNIDPYEVLNRQFWYDTANFHEPSLINSIETFGKDKFMMGS
                     DFPYFQDEKYTRGVDYIKNSNIDPDTINGILRGNAIEFYQIEENHNH"
     gene            complement(5921..6292)
                     /locus_tag="POH73_001679"
     CDS             complement(5921..6292)
                     /locus_tag="POH73_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDI6887625.1"
                     /translation="MLLRHVINTYVGSKIFKSSLPKVQNSDEMAQQFEQGFGLSRRSM
                     RLAGAFEMIGSLLLFTSIFGKLGKKLVVVGTIMINIVMGTAIYNHYKAGHGYKGAQAA
                     SKFFFLNVLSLLEVLSLTRNK"
     gene            complement(6312..7673)
                     /locus_tag="POH73_001680"
     CDS             complement(6312..7673)
                     /locus_tag="POH73_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HDI6887626.1"
                     /translation="MKQRNRVIAVVMIGAFVGVLNQTLMTTILPEIMKDFTVSSSTAQ
                     WLTTIFMLVNGIMIPITAFLIERFTLRSLFFNATCFLMIGSFICMLGINFPMLLLGRS
                     IQALGAGILMPLTQTLLFIMFPPEKRGMAMGMFGLVIGFAPAIGPTAAGWFVNIFDWR
                     YLFLVVLLIGMIDFVFGYLSLPNITELSKPNLDKLSVILSTVSFGGLLYGFSTAGNLG
                     WSHPMVYITIIAAIVILTVFIFRQLKLESPLLEFRVFKYNDFSVAMILIVLMFMLFIG
                     NLTILPIYMQTMMKWSPLESGLILLPGGLIMGLLSPVTGKLFDKIGGRILSIMGMLTI
                     MIGALLMAQFSQNTTQLYVIISFSVTMLGNAMIMTPMTTQALNALPRQYIAHGTAMNN
                     TIRQVSAAIGTGILVTLMTGLGKTSSLSGSQGLIHGLDITFYVVALIAFIGTIIAFFI
                     RKH"
     gene            7816..8019
                     /locus_tag="POH73_001681"
     CDS             7816..8019
                     /locus_tag="POH73_001681"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887627.1"
                     /translation="MKNAIMKEVVYINFEGLEQYLNGDNTRYNTLSLETLNYYLKQYV
                     LTVPFKNMDVQNGMKVSAEVKDI"
     gene            8029..8394
                     /locus_tag="POH73_001682"
     CDS             8029..8394
                     /locus_tag="POH73_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HDI6887628.1"
                     /translation="MNHQHGDFYYEMNNFFNVYFEYKGFTVNIVSSTIHTHGDGKSLK
                     GSHVSLIIPIDGVDYVTGADFGDLPFSVMPIVPKDNSPVIHDMNGDFHAIYVNDYLFY
                     VRKMGKDNDNNLDHTMKWN"
     gene            8669..9244
                     /locus_tag="POH73_001683"
     CDS             8669..9244
                     /locus_tag="POH73_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6887629.1"
                     /translation="MEKNQRKKRSDAEYNQQIILTTMEDLLEQGEDISTKKMSDIAKI
                     SGVGVGTLYRHFESKTLLCQAIMDKKVDQMFIEIEDILAENTQWPVRDKINVILTKYL
                     DLKEANFTTLNFIEKSSSHSSSVINIPFFERLKNLLIQQFENVHSIADLDFKLNLMLN
                     AFSSDFYYFVKHNQKLTKQQFLDKLLDLYLK"
     gene            9714..10187
                     /locus_tag="POH73_001684"
     CDS             9714..10187
                     /locus_tag="POH73_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6887630.1"
                     /translation="MEYKSYNKMSNNILSILLSEKNFDNKMVVSTGSYILSELPCIVA
                     YKNNDIVGLLTYKVYDEYIEIISLDSFVENKGIGSHLLNYAEIIASDMSKRSISVITT
                     NENIKALYFYQKNKYRITDVIFDAVTEARKIKPSIPEMGENGIEIRDEIVLKKCL"
     gene            complement(10294..11280)
                     /locus_tag="POH73_001685"
     CDS             complement(10294..11280)
                     /locus_tag="POH73_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDI6887631.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTCKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKLEL"
     gene            11589..11790
                     /locus_tag="POH73_001686"
                     /pseudo
     CDS             11589..11790
                     /locus_tag="POH73_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11839..12135
                     /locus_tag="POH73_001687"
     CDS             11839..12135
                     /locus_tag="POH73_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDI6887632.1"
                     /translation="MATEKDVNDLFLNHVNSNAVKTRKMMGEYIVYYDGVVIGGLYDN
                     RLLVKATKSARHQFQDNALVSPYPGAKEMILIPDFAEATNITNLFECIKNDLKN"
     gene            12343..12945
                     /locus_tag="POH73_001688"
     CDS             12343..12945
                     /locus_tag="POH73_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887633.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNKVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSEFLFLLVIFTFGIAGIIFLMPYGLCF
                     LPLYKQKKKKQTFKKYMAYTTIGLSIYLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKKIETVDNTNITER"
     gene            13213..>13856
                     /locus_tag="POH73_001689"
     CDS             13213..>13856
                     /locus_tag="POH73_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDI6887634.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAENFKNGAKQAQ
                     LQSFDEYFEIWNDERFIISEEKSEIGEIIFKFYRHCFRYNEINLKIQDIFDDYSDIHN
                     PNGTHCYGYTFNIDKHGQVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDIAYDEYFSVLNSKKDGLFAHYVAIEYVKDSDLPQP"
BASE COUNT         5098 a         1899 c         2176 g         4683 t
ORIGIN      
        1 agtataaaaa tgaaatagat aaaattaaat ctaaaatttt aaatgaagaa gacgagttag
       61 taaaaaaatc attgatgtta acaatatttg tattatcgga gagctatgtt agttcattaa
      121 ttgtttctaa tttacctgaa agagatgaaa atttaataga taaagcgtat gaaaaaatta
      181 ttcaacagta tataagtaaa aatatacgta caagaaatgg tagaaaagaa ctagtaaaaa
      241 atttttacaa tacagatcta ccagcaatac ctcataccaa attgagagat gcccttgctc
      301 acgaaataaa taaagttgat ttaaaaaaca atcgttttaa atatgataat agtagaggta
      361 atagaaatga aaatattata gaagttagta ttgacgaaat aattaatgag ttagaagaat
      421 ggtttttaaa tttagaattt ccgtaaaaac ctcatcatca accgataagc agaagcgtat
      481 cataagtagc ggaggagttt tttaccttgt gacttatcat aaagtacgat gtttatgtaa
      541 gtgattatca ttatttaagc aggtttttca aattaaataa taacaagaat aaaatgcact
      601 tagcgacatt gaaatttatt aatctagtaa actaatagat ttatagaaaa ttttatttgc
      661 aaggggataa ttttgaaaag tagtattttc tatctttcca taatacattg taattacaac
      721 ggaggggata ttgtgatgaa gtgtatagat aaaacgtggg ttagctatta taaagaatta
      781 gctgataagt taacagatta tcaaaataaa cgttatgaat taattgaaat agtgaaggaa
      841 gtatataaaa aaacgggaat aaaattccct actttagcaa gtgataatgt attgatggac
      901 atagatcctt ttacaatatt tgcattattt aataaaaatt ccatgagaga aactaataag
      961 gtaaaaatat taacagaatt agcttcggaa ttgaatatta agtccaaaat tccgtcagtt
     1021 tttgacagta ttccaacagt caataatctg aatgctacat attataattt taaagattat
     1081 ggaaaagata atgatataga gaccttgtgg accttattta ctgctgcatt aaattttgcg
     1141 aattatcctt cagaagataa taaattaaaa tttatacata gttttgataa gaatattact
     1201 aaaacaggaa atgggaatag taagattacg agtggtttgt actggattgc acctgaaact
     1261 tttgctaact ttgattcacg ttcaatttgg tacatttttg atacatataa actccctaaa
     1321 gaattgatta atgaacaact taaaccaaaa gaaaaactaa caggtcgaca ttatttagaa
     1381 ttgataaata tgttaaagca agttttgaag tgccaggaag tagagattaa tgattttatt
     1441 gatttaagtt atgagtcttg gcattattca gaaatgataa atgaagctaa tagaaacaaa
     1501 tttgattcta atatcaaaga aattgttgtc agtgaaaatg acaatataat acaaaataga
     1561 agtagaacaa ctaaagttat accatatgga aaacaagaat tcttagatga agtttttatt
     1621 gatgaaagtg attatgatag gttagtacaa cttttaagaa gaaagcgaaa tgtcattcta
     1681 cagggaccgc ctggtgtagg aaaaactttt cttgcaaaac gtcttgctta ttcattgatt
     1741 ggcagtaagt gtaaagaacg aattaaatta attcaatttc atcaaagtta ttcatatgaa
     1801 gattttatta tgggatatag acctactagt tcaggttttg aactgactac agggacgttc
     1861 tatgattttt gtaagaaagc tgataaagac cgcgataatg aatatttttt aattattgat
     1921 gaaattaata ggggtaatct gagtaaaatt tttggtgaat tatttatgtt gattgaaagt
     1981 gataaaagag atgaagaact aatattactt tatagcggtg agagattttc tgtacctaga
     2041 aatatttata ttgtgggaat gatgaataca gcagatcgta gtttagcaat tttagattat
     2101 gcattacgca gacgctttgc gttttataat atgcaaccgg cgtttcatac aactaaattc
     2161 aaaaaacttc ttaaagcaat taataatact gaattagagt cagtaattaa ttgtattgaa
     2221 ggattaaata aagagattgt tcaagacgat atgttaggta atggctttgt tattggtcat
     2281 agttatttta gcaatattga atgtatcgac aaagatgagc tttctaatat tatagaattt
     2341 gagattattc ctatgttaga agaatattgg tttgatgaac cttcaaaaat aaatgtatgg
     2401 tctgaaaagt taaggaattc aataaataat gattaatatt aaaaatattt attatatgtt
     2461 atcttatgca tttacagtac ttaacaaaaa aggctatcag aagttagcaa cagaacaatt
     2521 tgaaaatata tttgatttat attcagctat tcttataaaa ggtatatcaa gtcagttaaa
     2581 tagtggactt catcatgaat atatagaaca gacagattct ctaaaagtta ttaggggaaa
     2641 agttgatgtt aagaattcta tacaaggctt aggagtttta agtcaacgta ttaattgtat
     2701 ttatgatgaa ttttcattaa atacatacat gaacaagatt ttaaaaacta caatgaaatg
     2761 tttaataaaa acagatattt ctagaaagaa taagataaag ctccgaaagt tattagttca
     2821 ttttaataat gttgatactt tagattatag aaatattcaa tggtatcatt cgtttgatcg
     2881 aaataatcag acgtataaaa tgttaatatc tatatgctat ttaatttttc aaggagtaat
     2941 acaaactgaa agcaagggac aaaatgatct tatggtattt gtagacgaac aacaaatatc
     3001 tcggctatat gaaaaattta ttctagagta ctataaaaaa gaatttccag agcttgttgt
     3061 aacatcatca aatattcaat ggtcattaga taatgatgat aacgtgaata tgcttcctgt
     3121 aatgagaagt gatattatgt tgagatataa agataaatgt ctaattatcg atgcaaaatt
     3181 ttataaaaat acattacaca attattatga tactaaaaag atccattcaa ctaacctgta
     3241 tcaaatattt acttacgtga aaaaccaaca attaaattta aaaaagaaag caatacaggt
     3301 ctcaggaatg ttgttatatg ctaaaacaga cgaaaatatt gttttgaacg ataaatttca
     3361 tatgagtggt agtcaaatca ttattaagac attagatttg aactgtaatt ttactattat
     3421 taaaaaacag ttgaatggga tagttaatga tatattttta ctaaaataat attaaagatt
     3481 taaaattata aagatattaa ttatgattag tggtttcaaa atgttgtttt ttagaggttt
     3541 aagattgtca taaatttagt tttatttgtt ctcatgttca agtttcttta tatcaacttt
     3601 tattttagta aaccttaaag agtagcaact tcaaaattcg catttatatc taaaaataat
     3661 tttaaaatag acatgtttaa acaatcaaaa gtgtgcattg ttaaatcatc attttcaaaa
     3721 gtttattagc gaaatagata cgttagtctt aaaagtgatt agtcgtataa cagtaattgc
     3781 aatttgtgaa agttaatttt atagttgtat gaccaatttt taaataagat tattaggtaa
     3841 ggcataatag ccctgttaag tgataaacga tacaaaagtg atatatagga ggcgatatca
     3901 tgattactat tgattcaaag attaataaac aaatcgctaa aaacctttca cttgaaggta
     3961 tgtatgttac acgagaacag caaatacaaa ttcttcatgc aattaataat gagaaagaaa
     4021 ttacaaatga acttattcgt aaaatcgctt ttaaataatg aaatatatag caacttttca
     4081 aaaggcatta aactatcttg aaaagttgct tattaaataa taattgctca aataaaaaac
     4141 atataaatgc tcttaccaaa aaaatattcg taactaaaat attacaaagt aattaaaaag
     4201 tgtgattacc caaaaatcct ttttttattc agattgcctt aatattgctc ctaaatgttg
     4261 ctgtgcagta gtaaataata cgaatgtaat taacttgtca atagcggttc aaaaatgctg
     4321 ttttatggcg gtttgaaaat gtcgtttttt agcggtttaa gattgtcata tatttaatga
     4381 tttttcggtt ttataatctt ttaatcgatt tgattctcca gtaattttaa atacttttga
     4441 atggtgaaca aaacgatcaa ttatagctgc tgatgcaatc ttattactaa atgacaattc
     4501 ccaactcgaa aatgaaatat tacttgtaaa attaaaaagt attaaacaaa aaacgtaatt
     4561 atgctactga aatagctcaa aagaattatg aaattcgaaa aaagaattgc aatatgccat
     4621 aatgctatct cctttctaaa ttaactcatc gcttcataaa tacatttatt caaatttgta
     4681 aatcaattaa taccaatgtt gtatcattat aatctatcat ttattcctta cctctataaa
     4741 aaatgtctca aaaaacatat aaacaaatat aaccattcaa tcattaaagc ataaagaacg
     4801 ctacttcatt tccattaata tttatataga aagtaaagta gcgttattct aacttattta
     4861 gtttatacca taatataatg tctctagaat aaaacgctag tgattatggt tttcttcaat
     4921 ttgataaaat tcaatcgcat tgcctcttaa tataccatta atcgtgtcag ggtcaatatt
     4981 actgttttta atatagtcta cgccgcgtgt atatttttca tcttggaagt aagggaagtc
     5041 tgaccccatc ataaatttat ccttaccaaa tgtctcaata gagttaatca atgaaggttc
     5101 gtggaagttt gctgtatcat accaaaattg tcgatttaat acttcataag gatcaatatt
     5161 aaaggcattc caatcttcat aattgtcttt aatacgagtc atgaagaatg gtaaagcgcc
     5221 acctaaatgt gaaatatgga atttaatatt tggatatttt tgcggaattt catttttaat
     5281 caattgtaat gtaataaagg tagactctaa cggtgcacca ataacccatt ctaattgata
     5341 gtcattgact aacggacttt gagcgccaca acctgttgga tgaatataaa ttgtagcatt
     5401 gagctcattc atcgctgcaa agaaaggttc aaattgttta tcagcaacag agaccttatc
     5461 ttttactata gttggaatgg caatgcccac aaattcatct tttttcaaaa gggcttgggc
     5521 ttcttcaata gcttgatcga catagggtag tgagacagca ccatatgcca agaaacgatc
     5581 cggatattgt tgaactaatc tttcatataa gtcattaatt tcttgagcac tttggtgagc
     5641 ttcttcttta gtaccccatt gtggagattg tggtgtcgca gaaataattt gtaaatctac
     5701 gccagcatcg tccatcattt tgaagcgttt ctctaaatct tctttagacg cagattgatt
     5761 aatcccttta gcaacttcag taccttgact acctaatttg ccaagtttgt ctaaatagtc
     5821 ttcattccat aaatgtgcgt gtgtatcaat cgcttttgtt tcagtcatat tatcccctcc
     5881 attaaaaatt gtcattcata atttgcttat tgccaatagc ttatttatta cgagttaagc
     5941 ttaacacttc tagtaagctt aatacattta aaaagaaaaa tttagaagca gcttgtgcac
     6001 ctttataacc atgcccagct ttataatggt tataaatagc tgtacccata acaatattaa
     6061 tcataatcgt accaacaact actaattttt tccctaattt accgaaaata gaagtaaata
     6121 aaagaagtga accaatcatt tcgaaagcgc cagctaatct cattgatcta cgagataaac
     6181 caaagccttg ttcgaattgt tgagccattt catcactatt ttgtactttt ggtaaactgc
     6241 ttttaaaaat tttactacct acatatgtgt taatgacgtg tcttaataac attttaaaat
     6301 cctccaaata attaatgttt gcgaataaag aatgcaatga tagtacctat aaaggcaatc
     6361 aatgcgacga catagaatgt aatatctaga ccatgaataa gtccttgact accacttaat
     6421 gaagaggttt ttcctaatcc tgtcatcaat gtgaccaaga taccagtacc tattgcagct
     6481 gacacttgtc taatcgtatt attcatggct gtaccatgtg caatgtattg acgtggcaaa
     6541 gcatttaaag cttgagttgt cattggcgtc atgatcatag cattacctaa catggtaact
     6601 gaaaatgaaa taatcacata tagttgtgtt gtattttgac taaattgagc cattaataat
     6661 gcaccaatca taatcgttag catccccata atactcaaga tacgtccacc aattttatcg
     6721 aataatttac cagtgacagg cgataacagt cccataatta acccaccagg taataaaata
     6781 agtcctgact ctaacgggga ccatttcatc atcgtttgca tataaattgg taagattgtt
     6841 aaattcccta taaataacat gaacattagt acaattaaaa tcattgccac tgaaaagtcg
     6901 ttatatttaa atactctaaa ctctaacaat ggcgattcca gttttaattg tcggaaaata
     6961 aataccgtta atataactat ggcagcaata atagttatat aaaccattgg atgtgaccaa
     7021 cctaagtttc ctgctgtact aaaaccatat agtaagccac caaagctaac cgtcgataaa
     7081 ataacggata gtttgtctaa gtttggttta cttagttccg ttatatttgg caatgataga
     7141 tacccgaata caaaatcaat catacctatt agtaatacga ctaggaacag atagcgccaa
     7201 tcgaaaatat tgacgaacca accagctgca gttggtccaa tcgcgggtgc aaaacctatt
     7261 acaagaccga acattcccat tgccattcca cgtttttcag gtggaaacat aatgaaaagg
     7321 agtgtctgtg tgagtggcat cagtatacca gcacctaagg cttgaatact acgtcctaat
     7381 aataacattg gaaaattgat tcctagcata caaataaatg aaccaatcat taaaaagcat
     7441 gttgcattaa aaaataaaga acgtaatgta aaacgttcaa ttaaaaatgc tgtgatagga
     7501 atcataatcc cattgactag cataaaaatt gtcgtgagcc attgtgctgt actacttgat
     7561 accgtaaaat ctttcataat ttctggaagt atcgtagtca tcaaagtttg attcagcaca
     7621 ccaacaaaag caccaatcat tacgacagct ataacacggt tccgttgttt caaatctata
     7681 ttaaagcata tacattcctc aaattttgac gcctccctct cttttaagtg gaacctacta
     7741 tcatttgttt tatacggaat acgttccgtt tgtcaattta ataactcaag ggtatgataa
     7801 taggtatctg ataaaatgaa aaacgcaata atgaaggagg tagtttatat aaattttgaa
     7861 ggattagaac aatatttaaa tggtgataat acaagatata acacgcttag tttagagacg
     7921 cttaattatt atttaaaaca atatgtgtta actgtcccgt tcaaaaatat ggatgttcaa
     7981 aatgggatga aagtttctgc agaagtaaaa gatatttaaa ataatgtagt gaaccatcaa
     8041 cacggtgact tttattatga aatgaataat ttctttaacg tgtattttga atataaagga
     8101 tttactgtaa atatagtttc ttcaactatc catacccatg gtgatggaaa aagcttaaaa
     8161 ggttctcacg tgtctttaat catacctatt gatggcgttg attatgttac aggtgctgac
     8221 tttggagact tgccatttag tgtcatgcca attgttccaa aagataatag tccagtcatt
     8281 catgatatga acggtgattt ccatgcaatt tacgtcaacg attatttatt ttatgttcga
     8341 aaaatgggga aagataatga caataacttg gatcacacta tgaagtggaa ttaacacctc
     8401 gtcatattca tgactttgtt tacaatattg aatataatga aattaatcct aatgctattt
     8461 ttgtgaaacg ctagttagta acaatgatta aatctttttg gtcgtgctac aatgttacaa
     8521 aataatataa ctttaactaa gcaacaaaag aaagaataat atgatgtaac acctgagaat
     8581 gatagacaat ttttagaaga agcatttaat ttagatgtta aaattgatag attagaaagt
     8641 taaaaacaac gttaaaaaga ggaatataat ggaaaaaaat caacgaaaaa aacgcagtga
     8701 tgccgaatat aaccaacaga ttatcttaac tacaatggaa gatttactag aacagggtga
     8761 agatataagt actaagaaaa tgtcagatat tgctaaaata tcaggtgttg gagttggtac
     8821 attatatcgt cattttgaaa gtaaaacatt gttgtgtcag gctattatgg ataaaaaagt
     8881 cgatcaaatg tttatagaaa tagaagatat tttagcggaa aatacacagt ggcccgtgcg
     8941 agataaaatc aatgttatat taacaaagta tctagattta aaagaagcaa atttcacaac
     9001 attaaatttt atagaaaaat ccagttcaca ctctagttct gtaattaata ttccattctt
     9061 tgagcgacta aaaaacttat taattcaaca attcgaaaat gttcattcca tagcagactt
     9121 ggattttaaa cttaatctaa tgctcaatgc attttcttca gatttctatt attttgtaaa
     9181 acataaccaa aaattaacaa aacaacaatt tttagacaaa ttactagatt tatatctaaa
     9241 ataatatgtt ctgaaaaata gaagtatttt ttgaatcact aaataacgcg ctattaaatg
     9301 gcttcgctat gcccgtctaa cacattagtg taaaatttta taaattgaat ttttgatgac
     9361 gggtcccttc ctagggtgct ctctcagaca ccccaaccgg cacattattg taagttgact
     9421 tttcgataat attaaagatt taaaataata aagctattaa ttatgattag tggtttcaaa
     9481 atgctatttt tagaggttta agattgccat gaatttcgtt ttatttgttc tcatgttcag
     9541 gtttctttat atcaactttt attttagtaa accaaaaaga gtagcaactt caaaattcgc
     9601 atttatatct aaagataatt ttaaaatgca catgtttaaa gaatcaaaag tgtgcattgt
     9661 taaatcatca ttttcaacta ataaggatta ttaacagaga gaagtgactt attttggaat
     9721 ataaatctta caacaaaatg agtaataata ttttatctat cttgttgagt gaaaaaaatt
     9781 tcgataataa aatggtagtc tcaacaggta gttacatttt gtctgagttg ccatgtattg
     9841 ttgcctataa aaataatgat atcgtggggc tactaacgta taaagtttat gatgagtata
     9901 ttgaaatcat ttctttagat agttttgtag agaacaaggg tattggtagt catttactta
     9961 attatgctga aataattgcc tcagatatga gtaaaagaag tatcagtgtt attactacaa
    10021 atgaaaatat taaggcactc tatttctatc aaaaaaataa atatagaatc acagacgtta
    10081 tttttgatgc tgtaacagaa gctaggaaaa taaagccgtc gattcctgaa atgggtgaaa
    10141 atggcattga aattagagat gaaattgtat tgaaaaaatg tttataacgt ataaatatac
    10201 gttgttttaa atcaccctag ttcataccaa aaagacttta acacaaacaa aaaggaggaa
    10261 acttaaattc ctcctcaacc tttattactc atactataat tctagtttaa cgtcttcgtc
    10321 catttgggct tcaaattcat ctagtagtgc tcgtgcttct gcaattgatt gtgtgttcat
    10381 caattgatgg cgaagttcac tagcgcctct tatgccacgc acatagattt taaagaatct
    10441 acgtaaactc ttgaattgtc gtatttcatc tttttcatat ttgttaaata atgatagatg
    10501 caatctcaac agatctaata gttctttgca tgtgtgttcg cgtggttctt tttcaaaagc
    10561 gaatggatta tggaaaatgc ctctaccaat catgacgcca tcaatgccat atttttctgc
    10621 aagttcaagt cctgtttttc tatcgggaat atcaccgtta attgttaaca atgtgtttgg
    10681 tgcaatttcg tcacgtaaat ttttaatagc ttcgattaat tcccaatgtg catctacttt
    10741 actcatttct ttacgtgtac gaagatgaat agataaattt gcaatgtctt gttcgaagac
    10801 atgcttcaac caatctttcc attcatcgat ttcatagtaa ccaaggcgtg ttttaacact
    10861 taccggaagt ccacctgctt tagttgcttg aataatttcg gcagcgacgt caggtcttaa
    10921 gattaagccg gaacccttac cctttttagc aacatttgct acaggacatc ccatatttaa
    10981 gtcaatgcct ttaaatccca ttttagctaa ttgaatactc gtttcacgga actgttctgg
    11041 cttatctccc catatgtgag cgaccatcgg ctgttcatct tcgctaaaag ttaagcgtcc
    11101 gcgcacacta tgtatgcctt cagggtggca aaagctctcg gtatttgtaa attcagtgaa
    11161 aaacacatcc ggtctagctg cttcacttac aacgtgtcga aagacgatat ctgtaacgtc
    11221 ttccattggc gccaaaataa aaaatggacg tggtaattca ctccaaaaat tttccttcat
    11281 aatatattta taccctcttt ataattagta tctcgatttt ttatgcatga tgatattacc
    11341 acaaaagacg aacttataca aaaggaattt tagttgatac aaccatttga aagggaagtc
    11401 tacgagtagt ctaaaatgaa tgttgtggta agttgatcaa tatacaaatc aaggattatc
    11461 gcgttaaatt gttcattatt aatgatacac tacttattat tatgattcag aattttcttt
    11521 ggctacatat acagtaatgt gatttatacg ttatcttata acaaaggcaa atttataaag
    11581 gtgatattat ggaagattta aagcaatctt taaaaggttt aggttggtat gacttttttt
    11641 atagtaccta tgtttctact attcgtgtat ctgccgaatt ataattttat aactatattt
    11701 attaacattg ttatcattat tttcttttcc ataggtttga ttttaactac gcatataatt
    11761 atagataaaa ttaagagcaa ctcgaaatga ttcattaaca cgaatgtgat taaacataaa
    11821 actgaaggag cgattacaat ggcgactgag aaagatgtaa atgatttatt tttaaatcat
    11881 gtgaattcaa atgctgttaa aactagaaag atgatgggag aatatattgt ttattatgat
    11941 ggtgtggtta taggtggttt gtatgataat agattattgg tcaaggcgac taaaagtgca
    12001 cgtcatcaat ttcaagataa tgcattagta tcgccgtatc ctggtgctaa agaaatgata
    12061 ttaattccag actttgccga agcaacaaat attactaatt tatttgagtg cataaaaaat
    12121 gatttgaaaa actaatcata aattgccatg ctcaactatt ttgaaaagca tcatttccat
    12181 attatcaaat tattgagtat ctcgatttat tgttatacac aaacagtatt tttgttatca
    12241 tagagcttat aaatataata aattgaataa gtaactttac aaaactagaa tgattcaatt
    12301 caatcacaat tatttgaaaa tagaggtgag caggtgaacg atatgttaat tagtctcgta
    12361 atcccagttt ttgctttgtt agttattggt ggtattattt ggatgattat agaaggtata
    12421 gtacatattt caaaaaagaa taaagcaatt gataactttt ttaacaaagt taataaagta
    12481 agtgagacat ataaattcgc tactactttt ttatttctaa tcttggctac ggctggtatt
    12541 tctcaatttt atctatatta tatagtatca gagtttcttt ttttgctggt tatatttacc
    12601 tttggtattg caggtatcat ttttttaatg ccatatggct tatgtttcct accactttat
    12661 aaacaaaaaa agaaaaaaca gacatttaaa aaatacatgg cttacactac gattggtttg
    12721 tcaatttatc taggcttatc tctagttttg gttcacacta cgaaaattta tatggacgaa
    12781 ggtggcgtaa gatactatta cggtagtttt gtaatgaaac aagcgggcgg ttatgcttat
    12841 ttagctttag cagtactttc aacgttgtta attgttgcga aaaaagctac aaataaaaat
    12901 aaaaaaatcg aaaccgtcga caatacaaat ataacggaaa gataattaag agagtgctca
    12961 ttcaggagtg ctcttttttt gatgttcaaa tttagttgga aatgaaggca tagaagaaat
    13021 ttttatagtc atttattgga taatgaatta tggtcatatc gattaatttt attagtgagg
    13081 attttacaaa catttgttga ataaatatgg taatgataat atatgaatat ttttgaaaaa
    13141 acactaatat gattaaatta tttgttaaaa tagcattaag taaaaaacaa acagggaaca
    13201 aggtgggatt tcatgagtca attgctaaat gatacgttat cggcttggtt gttaattgaa
    13261 tctttaagtc caggagaagt aaattttaca gcggaagata tactctcagc tgaaaatttt
    13321 aaaaatggtg caaagcaagc gcaacttcaa agttttgatg aatattttga aatatggaat
    13381 gatgaacgct ttattatatc agaagaaaaa tcagagattg gcgaaatcat ttttaaattt
    13441 tacagacact gtttccgcta taatgaaatt aatttgaaaa ttcaggatat ttttgatgat
    13501 tattcagata ttcataatcc aaatgggaca cactgttatg gttacacatt taatatagat
    13561 aaacacggtc aagtgatagt tgattctata catattccga tgattatgag tgcattgaaa
    13621 gaaattgaaa agaataaaaa tgctaatata gaagaaaaat ttaatgattc tgttgaaaaa
    13681 tttgttcaaa aagtaaaaga aattttagcg gatgaaccaa ttaatgaatt taaattgaag
    13741 aagatggaca tagcttatga tgagtatttt tctgtattaa attcaaagaa agatggatta
    13801 tttgcacatt atgtagcaat agaatatgtg aaagatagtg atttaccaca gccgga
//
LOCUS       DAMZPP010000077        13822 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMZPP010000077 DAMZPP010000000
VERSION     DAMZPP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13822
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.077"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..773
                     /locus_tag="POH73_001690"
     CDS             <1..773
                     /locus_tag="POH73_001690"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDI6887635.1"
                     /translation="MALPTKAMREVATQINDKLTSKKTFIHVAKGIENGTFKRVSEMI
                     EDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKNVSKLTQDLFMNDYLRVYT
                     NDDLVGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTRGLAEISRLGEKLGADPMT
                     FLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSEMNMVVEGIYTTKSVYHLA
                     KEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            944..1216
                     /locus_tag="POH73_001691"
     CDS             944..1216
                     /locus_tag="POH73_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDI6887636.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
     gene            1647..2219
                     /locus_tag="POH73_001692"
     CDS             1647..2219
                     /locus_tag="POH73_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HDI6887637.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDSYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            2222..2947
                     /locus_tag="POH73_001693"
     CDS             2222..2947
                     /locus_tag="POH73_001693"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HDI6887638.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGMKALDVCCGTGDWTIALSKAVGSTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEADFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            2949..3908
                     /locus_tag="POH73_001694"
     CDS             2949..3908
                     /locus_tag="POH73_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDI6887639.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQLSTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKEVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            4000..4449
                     /gene="ndk"
                     /locus_tag="POH73_001695"
     CDS             4000..4449
                     /gene="ndk"
                     /locus_tag="POH73_001695"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDI6887640.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
     gene            4918..6084
                     /gene="aroC"
                     /locus_tag="POH73_001696"
     CDS             4918..6084
                     /gene="aroC"
                     /locus_tag="POH73_001696"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDI6887641.1"
                     /translation="MRYLTSGESHGPQLTVIVEGVPANLEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISDEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLEQLDIEIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDGIAQAMRDKIDEAKNDGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGRIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNTELGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLQQQIADRRQLNVEF"
     gene            6110..7174
                     /locus_tag="POH73_001697"
     CDS             6110..7174
                     /locus_tag="POH73_001697"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDI6887642.1"
                     /translation="MKLQTTYPSNNYPIFVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFNDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFVAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLKGMDKYIAKGI
                     ETKLDIVVADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            7184..8482
                     /gene="aroA"
                     /locus_tag="POH73_001698"
     CDS             7184..8482
                     /gene="aroA"
                     /locus_tag="POH73_001698"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDI6887643.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVDIKEDEDKLVVNSPGYKAFKTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKSMNANIEGIEDNYTPLIIKPSVIKGIN
                     YKMEVASAQVKSAILFASLFSKEATIIKELDVSRNHTETMFRHFNIPIEAEGLSITTI
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGVNPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPIKIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            8489..9733
                     /locus_tag="POH73_001699"
     CDS             8489..9733
                     /locus_tag="POH73_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887644.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFIWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQMEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            9747..10322
                     /locus_tag="POH73_001700"
     CDS             9747..10322
                     /locus_tag="POH73_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDI6887645.1"
                     /translation="MSETLNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKHNIKLMNTNEIFDYIANHNGAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDD"
     gene            10312..10899
                     /locus_tag="POH73_001701"
     CDS             10312..10899
                     /locus_tag="POH73_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HDI6887646.1"
                     /translation="MTINKLWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     QFKIFVPDSPTAILFLVISISLILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMTWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            10971..11651
                     /locus_tag="POH73_001702"
     CDS             10971..11651
                     /locus_tag="POH73_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDI6887647.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            11987..12304
                     /locus_tag="POH73_001703"
     CDS             11987..12304
                     /locus_tag="POH73_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDI6887648.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     KRK"
     gene            12548..13690
                     /gene="bshA"
                     /locus_tag="POH73_001704"
     CDS             12548..13690
                     /gene="bshA"
                     /locus_tag="POH73_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HDI6887649.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQKTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDSDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            13695..>13822
                     /locus_tag="POH73_001705"
     CDS             13695..>13822
                     /locus_tag="POH73_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDI6887650.1"
                     /translation="MDKSLFEHARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDI"
BASE COUNT         5015 a         1862 c         2597 g         4348 t
ORIGIN      
        1 taatggcttt acctactaaa gcaatgcgag aagttgctac tcaaattaat gataagctga
       61 catctaaaaa gacttttata catgttgcta aaggtattga aaatgggaca tttaaacgtg
      121 tgtcagaaat gattgaagat tctatttcac ctgaatataa tgcaggtatt ggcgtgttgt
      181 cagggccaag tcatgcggaa gaagttgtag tcaagcaacc aactacagtt gctgcatcat
      241 caaaagataa aaatgtaagt aaattaacgc aagatttatt tatgaatgat tatttgcgtg
      301 tgtacacgaa tgatgacttg gttggtgttg aacttggtgg tgcattgaaa aatatcatcg
      361 cagtagcaag tggtatcgta gctggaattg gctacggtga taatgcaaaa gctgcattaa
      421 tgactcgtgg cttagcggaa attagcagat taggtgaaaa gttaggtgcc gatcctatga
      481 catttctagg tttaggtggt atcggtgact taatcgttac ttgcacatca acacattctc
      541 gaaatttcac attaggatat aagcttggac aaggtgaatc gatggatcaa gcattatctg
      601 aaatgaatat ggttgttgaa ggtatttata caactaaatc cgtttatcat ttagctaaag
      661 aaaaaaatgt ggatatgcca attacaaatg cattatatag agtattattt gaaaatatct
      721 cagtaaaaga atgcgtaaaa gatttaatgg agcgcgataa aaaatctgaa taaaatgtgt
      781 attattacac atatacagct aataattacg taaaatgaat agaaatggct taaattcaac
      841 gtttttgcca cataaatgat tgcgatttat gtaatgcttg tgttaaagtt ttaccataat
      901 gatataaaca tatcattatc aaacctcagg aggtgaatgt ctaatgaaca aaacagattt
      961 aatcaatgca gttgcagagc aagctgattt aactaaaaaa gaagctggtt cagcagtaga
     1021 tgctgtattc gaatcaatcc aaaactcact tgctaaaggt gaaaaagtac aattaattgg
     1081 tttcggtaac tttgaggtac gtgaacgtgc tgcacgtaaa ggtcgtaacc ctcaaactgg
     1141 taaagaaatt gatatcccag caagtaaagt tccagcattc aaagctggta aagcattaaa
     1201 agatgctgta aaataatttt acttaaaaag ccctgaataa gggcttttta ttttgctttt
     1261 aatacttaca acctgtacat aaattgtaat gttcttctaa gtttttaatc tttggaacat
     1321 tcattgttta agcgttcaaa atattacatg acataatagt tttgataaaa atagcaacta
     1381 caaaatatac atatgaataa tgagattaat tgttagcgtt gagtttactt aaaaggtaac
     1441 catgtctact atagtatttt acgttattta aaaagatgaa taatgtaaat gaagtaaggg
     1501 ttattatgag aattacaaaa gctacataaa ttaccgttag tttaaattga aatttaaaaa
     1561 tgataagtaa tcttaatgct tcaaaagatt ttaatttctt acatataatg ttaatatgaa
     1621 tagtgataat atcgtgaggt gtagacatgg aaacaactgt tagcaaattg gaaagacaaa
     1681 tagaagaaag attaaagggt gtatcagaat atgaatccat taatattaat catcggttag
     1741 gtaagttgct tgattcatat gatattccag atgttgccaa agtagcatgc ctcacaattg
     1801 acacatcaat gagacacctt gatgacatta catataatca tttgtctaaa cattccattt
     1861 tgattggaga tttaattagt gcacattttt atacgctact agcagaaatc aatgatttat
     1921 catttcaaaa tgaaattagt aaagcaattg ttgaaattaa tgaattgaaa tcttcattac
     1981 atcatcaagc tttaaatgat tatgaaattt ctcaagcaat agtaaaaatt gaaacacttt
     2041 tcccatatat tacgttatct catttcggta tcaatattga tgagtcggaa atatataatt
     2101 atttatttga agatatgtct gattactatc catcatactt caagaaatat aatcaaagtg
     2161 aagtaaagca ttacttacac gatatccaaa aaagttattt aaaaagtaga ggtaattaag
     2221 catggctgac aataaagcaa ataaagagca agtacatcgc gtttttcaaa atatttcaaa
     2281 aaaatatgac agattaaata atattattag ttttgagcag cataaagtat ggagaaaacg
     2341 tgtcatgaaa gacatgggtg ttagaaaagg tatgaaagca ttagatgttt gttgtggtac
     2401 tggtgattgg acaatagcat taagcaaagc tgtaggatca acaggagaag ttactggcat
     2461 tgactttagt gagaatatgt tagaagtagg aaaagaaaaa actgcttcaa tggaaaatgt
     2521 taaacttgtt catggtgatg caatggaatt gccgtttgaa gacaattctt ttgattatgt
     2581 aacaattggg tttggattaa gaaatgtgcc agactattta gtcgcgttaa aagaaatgaa
     2641 tagagtactt aaaccaggtg gtatggtggt atgtcttgaa acgagccaac caactttgcc
     2701 agtctttaaa caaatgtatg cattatactt taaatttgtt atgcctattt ttggaaaatt
     2761 atttgcaaaa tcaaaagaag aatatgaatg gttacagcaa tctacgttta attttcctgg
     2821 aaaagaagag ttgaagcgca tgtttgaaga agcggatttc ataaatgtaa gagtacgtag
     2881 ttttacaggg ggcgttgctg caatgcacct tggctataaa gaaaaagata ataccaaagg
     2941 tgattaacgt ggcaaagtta aacatgaaca atgaaattaa gaaagtggaa caacgacttg
     3001 aaaaggcaat aaaaagtaaa gattctgtat tagaacaggc atcattacac ttattgtctt
     3061 ctggtggtaa aagagtacgc ccagcatttg taattctgag tagccaattt ggcaaagatg
     3121 aacaaacgtc tgaacaaacg tatcaagttg cagtcgcatt agagttaatt catatggcaa
     3181 cacttgttca tgatgacgtt attgataaaa gcgacaagcg tcgaggcaag ttaaccatat
     3241 caaagaaatg ggatcagaca actgctattt taactgggaa ttttttattg gcattaggac
     3301 ttgaacactt aatggcagtt aaagataatc gtgtacatca attaatatct gaatctatcg
     3361 ttgatgtttg tagaggggaa cttttccaat ttcaagacca atttaacagt caacagacaa
     3421 ttatcaatta tttacgacgt atcaatcgca aaacagcact gttaattcaa ttatcaactg
     3481 aagttggtgc aattacatct caatctgata aagaaactgt acgaaaattg aaaatgattg
     3541 gtcattatat aggtatgagc ttccaaatca ttgatgatgt attagacttc acaagtaccg
     3601 aaaagaaatt aggtaagccg gtcggaagtg atttgcttaa tggtcatatt acgttaccga
     3661 ttttattaga aatgcgtaaa aatccagact tcaaattgaa aatcgaacaa ttacgtcgag
     3721 atagtgaacg caaagaattt gaagaatgta tccaaatcat tagaaaatct gacagcatcg
     3781 atgaggctaa ggaagtaagt tcgaagtatt taagtaaagc tttgaatttg atttctgagt
     3841 taccagatgg acatccgaaa tcactacttt taagtttgac gaaaaaaatg gggtcaagaa
     3901 acacatagta tttatgaaaa gtattgaaag cgctttacca acctgttaat atataatagt
     3961 aatatacata aacctattaa acacaggggg atacataaag tggaacgtac atttttaatg
     4021 attaaaccag acgcagtaca aagaaatcta attggtgaag taatttcaag aattgaacga
     4081 aaaggactaa aacttgtcgg tggtaaatta atgcaagtac caatggaact tgctgaaaca
     4141 cattatggtg aacaccaagg taaaccattt tataatgatt taatttcatt tattacatca
     4201 gcaccagtgt tcgcaatggt agttgaaggt gaagatgcag ttaatgtatc tagacatatt
     4261 attggcagca ccaatccttc agaagcttca ccaggatcaa ttagaggtga tttaggttta
     4321 actgttggta gaaatatcat tcacggttca gattcattag agtctgctga acgtgaaatt
     4381 aatttatggt ttaatgaaaa tgaaattact agctatgctt caccacgtga tgcatggtta
     4441 tatgaataaa atataagcta taaaccttta tgattaataa aaggaaaaag ggttttgtta
     4501 tgtggttagt gatttatgac cattcataac aaaaccctat ttttatacgg actgattaac
     4561 tttataaaaa atgatcagtc cattttcttt tatataaaga aatatagctt gtatattatt
     4621 tattcgtggt tagctacgat tatatgcggt ataagttgtt cgaataaatt ttaaattttc
     4681 aaatggttgt ttaaacaatc taacaatatt agaaattcat aatacttttc tcgttgtgaa
     4741 tatattataa tgaatcttag ttcgtttaat attaagataa ttctgacatt taaaaagaga
     4801 tataaatcga tttcttaatt gagcttgaaa acaaacattt atgaatgcac aatgaatatg
     4861 ataagattaa caacatatta taatgttatc gtggaagtat gaaaggagcg agtgtgtatg
     4921 agatacctaa catcaggaga atcacatgga cctcaattaa cagttattgt tgaaggtgta
     4981 cctgcaaatt tagaaattaa ggttgaggat attaataaag aaatgtttaa gcgtcaaggc
     5041 ggttacggac gtggacgtcg tatgcaaatt gaaaaagata cagtggagat tgtttcgggt
     5101 gtaagaaatg gttatacatt aggtagccct attacaatgg ttgttactaa tgatgatttt
     5161 acacattggc gaaaaattat gggcgctgcg ccaataagcg atgaagaacg agaaaatatg
     5221 aaacgtacaa ttacgaagcc aagaccggga catgcagatt tagttggcgg tatgaaatat
     5281 aatcatcgtg acttacgaaa tgtattagaa cgttcatctg ccagagaaac agcagcacgt
     5341 gtagcggtcg gtgcactatg caaagtttta ttagaacaat tagatatcga aatatacagt
     5401 cgtgttgttg agataggtgg cattaaagat aaagattttt atgattcaga aacatttaaa
     5461 gcaaaccttg atcgaaatga cgtccgtgta attgatgatg gcatcgcaca agcaatgcgc
     5521 gataaaattg atgaagcgaa aaacgatggt gattcaatag ggggcgtagt tcaagttgta
     5581 gttgaaaata tgcctgttgg tgtaggtagt tatgtacatt atgatcgtaa attagatgga
     5641 agaatagcac agggtgttgt tagtattaat gcatttaaag gtgtaagttt tggagaagga
     5701 tttaaagcag ctgaaaagcc tggtagcgaa attcaagacg aaattctcta caatactgaa
     5761 ttgggctatt atcgtgggtc aaatcactta ggtggtttag aaggcggtat gtcaaatgga
     5821 atgccaatta ttgtaaatgg tgtaatgaaa cctattccta cgttatataa accattaaat
     5881 tcagttgata taaatactaa agaagacttt aaggcaacaa ttgaacgttc tgatagttgt
     5941 gctgtacctg cagcaagtat agtgtgtgaa cacgttgttg catttgaaat agcgaaagca
     6001 ttacttgaag agtttcaatc aaaccatatt gaacaacttc aacaacaaat tgctgaccgc
     6061 agacaattaa atgttgagtt ttaacaacga gaacaattga ggtgtgatca tgaaattaca
     6121 aacaacctat ccttcaaaca attatccaat atttgttgaa catggtgcaa ttgaccatat
     6181 tagcacgtat attgatcagt ttgatcaaag ttttatatta attgacgagc atgtaaatca
     6241 atattttgct gataaattta atgatatttt atcatatgaa aatgtgcata aagtcattat
     6301 tccagctggt gaaaagacga aaacatttga gcaatatcaa gaaacattag aatacatttt
     6361 gtctcatcat gtaacacgta atacagcgat tatagctgtt ggtggtggtg cgacaggaga
     6421 ttttgcagga tttgtagcag caacactatt aagaggtgtc cattttatac aagttcccac
     6481 aacgattttg gcgcatgatt ctagtgttgg cggtaaagtg ggtattaact caaagcaagg
     6541 taaaaacctt atcggtgcat tttatcgtcc aactgctgtt atttatgatt tagacttttt
     6601 aaagacgtta ccatttgagc aaatattaag tggctatgca gaagtttata agcatgcgtt
     6661 attgaatggt gaatcagcga cgcaagatat cgaacagcac tttaaagata gagagatatt
     6721 acagtcatta aaaggtatgg ataaatatat tgctaaaggt attgaaacga agctagatat
     6781 tgttgttgca gatgaaaaag aacaaggtgt acgtaaattt ttaaatttag gtcatacatt
     6841 tgggcatgct gttgaatact atcataaaat accacatggt catgcagtga tggttggcat
     6901 tatctatcaa tttatagttg cgaatgcttt atttgattct aagcatgata ttaatcatta
     6961 tattcaatat ttaatacaac ttggctatcc tttagacatg ataactgact tggattttga
     7021 aacgttatac caatatatgc taagtgataa aaagaatgat aagcaaggtg tacaaatggt
     7081 cttgattaga caatttggag atatcgttgt acaacatgtt gatcaactaa cattacaaca
     7141 tgcatgtgaa caattaaaaa catattttaa gtaggtgaat gaaatggtaa atgaacaaat
     7201 cattgatatt tcaggtccgt taaagggcga aatagaagtg ccgggcgata aatcaatgac
     7261 acaccgtgca atcatgttgg cgtcgctagc tgaaggtgta tctactatat ataagccact
     7321 acttggtgaa gattgtcgtc gtacaatgga tatattccga ttattaggcg tagatattaa
     7381 agaagatgaa gataaattgg ttgtgaattc acctggatat aaagctttta aaacacctca
     7441 tcaagtttta tatactggaa actctggcac aacgactcga ttgttagctg gtttgttaag
     7501 tggtttaggt attgaaagtg ttttgtctgg cgatgtttcc ataggtaaaa ggccgatgga
     7561 tcgtgtttta agaccgttaa aaagtatgaa tgcaaatatt gaaggaattg aagacaatta
     7621 caccccatta atcattaaac catctgtaat taaaggtatt aattacaaga tggaagtggc
     7681 aagtgcacaa gtgaaaagtg ccatcttatt cgcaagtcta ttttctaagg aagcgaccat
     7741 cattaaagaa ttagatgtaa gtcgaaatca tactgagacg atgttcagac attttaatat
     7801 tccaattgaa gcagaagggt tatcaattac tacaatccct gaagcaattc gatacattaa
     7861 acctgcagat tttcatgttc ctggcgatat ttcatctgca gcgttcttta ttgttgcagc
     7921 acttatcaca ccaggaagtg atgtaacaat tcataatgtt ggagtcaatc caacacgttc
     7981 aggtattatt gatattgttg aaaaaatggg cggtaatatt caacttttca atcaaacaac
     8041 tggtgctgaa cctactgctt ctattcgtat tcaatacaca ccaatgcttc aaccaataaa
     8101 aatcgaagga gaattagtgc caaaagcaat tgatgaactg cctgtaatag cattactttg
     8161 tacacaagca gttggcacga gtacaattaa agatgccgag gaattaaaag taaaagaaac
     8221 aaatagaatt gatacaacgg ctgatatgtt aaacttgtta gggtttgaat tacaaccaac
     8281 taatgatgga ttgattattc atccgtcaga atttaaaaca aatgcaacag tggatagttt
     8341 aacggatcat cgaataggaa tgatgcttgc agttgcttct ctactttcaa gcgaacctgt
     8401 caaaatcaaa caatttgatg ctgtaaatgt atcatttcca ggatttttac caaaactaaa
     8461 gcttttagaa aatgagggat aatataaaat ggaagatatc tataaattaa tagacgatat
     8521 caatctacaa aaactagaaa atttagactc tcgtgttaat gaagcaataa ctactgacaa
     8581 cgatgacgca ttatttattc taggagagac actttacaat tttggattaa tgccacaagg
     8641 tttagaagta ttccgcgtgt tatatcacaa atatccagac gaaagtgaat tgctgattta
     8701 ttttattgaa ggtttaatgt ctgaaaatca aactgacgaa gcgttagaat atttaagtta
     8761 tgttgaacca tcacctgaaa agttgatgtt agaagcagat ttatatcaac aaattaatat
     8821 gatggaagtt gctattgata aattacaaga agcacttgaa ctagagccaa atgatccaat
     8881 aatccatttt gcgttggctg aaatgttata ttatgatggt caatatttac gtgctacctc
     8941 tgaatacgaa actgttttag aaactggtga atatcaagtt aatggtgtaa acttattctc
     9001 tcgtatggca gattgtagct tacaaagtgg taactatagt gatgcgattc gcttatacga
     9061 tgaaattaat gaagatgaaa tgacttcaga agattatctc aaaaaagcca tttcttacga
     9121 taaaaatgac atcactcaag aagcaattaa aataatgact acattacttt ctaaagatcc
     9181 tgattatatt caaggctact tgtatttaca atcattatat gaaaatgaaa aaaattatcc
     9241 agatgccatt gaaacgggta aagagggatt acgactaagt caattttata aagagttaat
     9301 gtatacaact ggatgtttgg aaatagaaca cggcgatgct aatgaaggtg tattattgtt
     9361 aaaacaagca ttagaggttg ataatgctta ccaagaacca ttattgattt taagcgattt
     9421 atatcgtaat gaagaagatt atgaagcaat cattgaatta ttaacatatg tagatgaaga
     9481 agatttggat cctacattca tctggcattt agcgttcgcc tatggtcaag aagagcgaga
     9541 taaagaagct caacatttct ttgaattagc atatccaact atgaaaacaa atattgattt
     9601 tatgagtgat tattatttct atttaattga aattggtcaa atggaacaag caattactat
     9661 tttaaatcaa ttgttggaac tagaaccaag taacgaaaat tggcatgacg aattatcaag
     9721 attacaatct taaaatgggg tatttgatgt cagagacact caatcaaatt aaagaaagtt
     9781 ttattgaata tttacttttt caatatcgat ttaagtcgcg tattgcagtt tgggtgctta
     9841 actatataaa agtgaatgag gcgaaattgg caaacattca ttttgtagat acaaaaatta
     9901 atcatcatga aacattagaa attgcagaag tgggaagtca cgcttctgca attcaattta
     9961 ctaagcataa tataaagtta atgaatacaa atgaaatatt tgattatatc gctaatcata
    10021 acggtgcttt tgatattcaa attcacttcg caaacgtgtc aaagcgtgag caaaggctag
    10081 atgatttaat agttgctcaa ctaacggaat cgcccagcta tcaaacttat cttcatgatt
    10141 taaatagtat ggctatagat cggcataaac atgcgttatt aatagattac ttgctacata
    10201 atattgattt aagtttacag atgaatgaaa agcaacgttt ttatcaacta acgcaaattt
    10261 taaatacact caagctagta aataaacata atcaatttga ggacttagca gatgacgatt
    10321 aataaacttt ggcaatatac tctttatcaa cgttcttggc taatgatgct actcatttgt
    10381 aatatattag ggatgattta tggttatata tggtatggtg aacaattaag tcatacgccg
    10441 tggcagttta agatttttgt tccagatagc ccaactgcaa tattattctt agttatttca
    10501 atctcattga tattaataca aaaacaaaat agtattatcg atgcactggc atttgtaaca
    10561 ttattcaaat atgggatttg ggcagttatt atgaatatat tatttattat tgagcaaggt
    10621 gatattacag taaatggcct ggtattaatg ttttcccata gtataatggc tgttcaggca
    10681 atttacttct atcctaggtt taaacgttct atgattggaa ttagtgtagc gatgacttgg
    10741 gtatttttga atgattatat tgactatttt caccttcaat ttccatatta tgattttata
    10801 actacacatg tgtggcaaat tggtgtatta tcatgttgtc tgagtgtttt tggacttttg
    10861 ctttatattg aattaaacaa attattaaag tgtaaatgat ttgataaaaa ctgcaaatga
    10921 cagtaaaata tttataaagg agagtgagtt tattgtcttc aatttttact atgataattt
    10981 atttcgtaat attaatggtg cttcctttat gggcacaaca caaagttaaa tctaactatg
    11041 agaaatattc acaagttaga tcaactagtg gtaaaacggg tcgtgaagtt gcattagaga
    11101 ttttacatgc aaacggtatt tatgatgtcg aagttgttaa aggtgaagga ttcttaacag
    11161 atcattacga tcctaaaaag aaagttgttt cactatctcc tgcaaactat gacagaccat
    11221 cagttgctgg aactgcaatt gcagcccacg aagttggtca tgccattcaa gatcaccaag
    11281 gatattggtt cttaagattc agagcagcat tagtacctgt tgcaaaccta gggagttcat
    11341 taagttatat gatcatcatg ttaggtatta tcttaacagc gataggtagt gcatttggtt
    11401 caactgcttt atggattggt gctggtttaa tgtcattagc ggtattattc tcaattgtta
    11461 cgttaccagt tgagtttgat gcgagttcaa gagcgatgaa acaaattacg gcattgaaca
    11521 ttgtaaatga aaaggaatac aaacatgcgc gtaaagtatt atctgcagca gcaatgactt
    11581 atgtagcagc aactgctgtc gctgtagcag agcttgtacg tattattttg attgcgcgtt
    11641 caagtgatta atcttataga ttaacaatta acttaaatta gacacttatt aactagctaa
    11701 tcacattttg tgtggttgct agtttttttg tgcttatttt tctaatggta gattattcaa
    11761 tatattattt tattgtagtg gtacgaatca taaagttata atctcaattt caattgattt
    11821 aatttagtta ggaatgcatt acatttatat aaactgaatt attaaactac taaaaaaaac
    11881 aacttgtcat ctagtttagc tagagtcagt agattgcact aaacatgcat gagacttcaa
    11941 attgatatat actatattag aaatgcaata taaggagaac gtagaaatga aatcaatggt
    12001 agaaatgcaa cgtgaagttg atgaatatat tggacaattt aaaacaggat atttttcacc
    12061 attagctaac ttagctagat tgactgaaga agtgggcgaa cttgcacgtg aaataaatca
    12121 tacctatggt gaaaaaaaga agaaagattc agaggaagca aatacgatta aagcagaatt
    12181 aggtgataat ttatttgtgt tgttatgttt agcgaattca atgggaatag atatgacaga
    12241 gagctttaat gaaacaatgg aaaagtttaa tacaagagat aaaaatcgat tcaaaagaaa
    12301 gtgaatataa aaatatatgc tttatttgga tttgattaag attaaaaaca tagactaatg
    12361 aattgcatcg catgaaatca tattattttt aaagtaagta taactagata aatgattata
    12421 tatacaacac ctgtttttta gatatttgaa attataaact caacattact ataaacttaa
    12481 atttaaacaa ttatctacgc atcgagaata atagaaagag aaatagaaag aaaggatggg
    12541 caagcgaatg aagataggta taacatgtta cccgtccatg ggtggctctg gaattattgc
    12601 cacagaatta ggaattaaat tagcagagcg aggtcacgaa gtacatttta tcacttcaaa
    12661 tatcccgttt agaataagaa aaccattacc gaatatgatt tttcatcaag ttgaagtgaa
    12721 tcaatatgca gtttttcagt atccaccgta tgatattacg ttaagtacta aaatcgctga
    12781 agttattaaa gaatatgatc ttgatttgtt gcatatgcac tatgctgtac ctcatgcaat
    12841 ttgtgggatt ttagctcgtg agatgtcagg taaagatata aaaattatga caacgctaca
    12901 cggtactgat attactgttt tgggctatga tcattcactc caaggtgcaa ttaaatttgg
    12961 cattgaaaaa agtgatattg tgacaagtgt gagtaaatct ttagcgcaag aaacacatga
    13021 aattatcgaa actaacaaag aaattatacc gatttataat tttgtacgtg aaaatgagtt
    13081 tccaactaaa cataacacag cattaaaatc acaatttggt atagcaccag atgaaaaagt
    13141 gttaatacat gtatcgaatt ttagacaagt aaaacgcata gatactatta tagaaacatt
    13201 tgcaaaagta cgtgaaaaga tacctagtaa attaatttta cttggtgatg gtcctgaatt
    13261 agtgccaatg cgtcaaaaga cgaaagagtt aaacgtagaa gaagatgttt tatttttagg
    13321 gaaacaagat tgcgtaagtg aattttacca attatctgac ttagtattat tattaagtga
    13381 aaaagaaagc tttggactaa ctttacttga ggctatgaaa acaggcgtcg ttccaattgg
    13441 ttcaaatgcc ggtggaatta aagaggttat taaacatggt gaaactggat ttgtcgtaga
    13501 tgtgggtgat agtgattcag cgagtgacta tgctatccga ttacttgaag acaaggtgtt
    13561 atacaacaaa cttcagaaaa acatgctagc agatattgct gaacgttttg gatcagaact
    13621 cattacagat caatatgagt attactatca aaaaatgcta aatgagcata acaaaagtaa
    13681 aggcgagtaa taatatggat aaatcattat ttgaacacgc aaggcctata ttagaacaaa
    13741 ttcaagacaa tggttttgaa gcatattatg taggtggctc tgtaagagat tatgtcatgg
    13801 gaagaaatat tcatgatata ga
//
LOCUS       DAMZPP010000078        13425 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMZPP010000078 DAMZPP010000000
VERSION     DAMZPP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.078"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(725..1171)
                     /gene="perR"
                     /locus_tag="POH73_001706"
     CDS             complement(725..1171)
                     /gene="perR"
                     /locus_tag="POH73_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HDI6887651.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(1268..2218)
                     /locus_tag="POH73_001707"
     CDS             complement(1268..2218)
                     /locus_tag="POH73_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDI6887652.1"
                     /translation="MKVVGLNRMREVETELQQRFSDVDFKFYKKASEIPESDLANLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIVINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSRVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            complement(2224..2679)
                     /locus_tag="POH73_001708"
     CDS             complement(2224..2679)
                     /locus_tag="POH73_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDI6887653.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIVYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKYGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2760..4049
                     /locus_tag="POH73_001709"
     CDS             2760..4049
                     /locus_tag="POH73_001709"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDI6887654.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGDAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHSNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            4342..5436
                     /locus_tag="POH73_001710"
     CDS             4342..5436
                     /locus_tag="POH73_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDI6887655.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(5627..7363)
                     /locus_tag="POH73_001711"
     CDS             complement(5627..7363)
                     /locus_tag="POH73_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HDI6887656.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATEEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(7654..8196)
                     /locus_tag="POH73_001712"
     CDS             complement(7654..8196)
                     /locus_tag="POH73_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDI6887657.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKDRYKKIRQY"
     gene            complement(8499..9536)
                     /gene="mutY"
                     /locus_tag="POH73_001713"
     CDS             complement(8499..9536)
                     /gene="mutY"
                     /locus_tag="POH73_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDI6887658.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFKSEHARREMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGTINIERLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
     gene            9688..10665
                     /locus_tag="POH73_001714"
     CDS             9688..10665
                     /locus_tag="POH73_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6887659.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTHPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVVGLHPLAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQKLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTKDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            complement(10926..11762)
                     /locus_tag="POH73_001715"
     CDS             complement(10926..11762)
                     /locus_tag="POH73_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887660.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRLIALIVFAVLMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGICVLCFLVLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSKDSTDEADT
                     SS"
     gene            complement(11771..13288)
                     /locus_tag="POH73_001716"
     CDS             complement(11771..13288)
                     /locus_tag="POH73_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6887661.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAQDIDLNNISLHI
                     YQGEALGIIGEPESSKAMVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINNHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDLQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPFMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            complement(13303..>13425)
                     /locus_tag="POH73_001717"
     CDS             complement(13303..>13425)
                     /locus_tag="POH73_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1811 family protein"
                     /protein_id="HDI6887662.1"
                     /translation="LTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALLQL"
BASE COUNT         4461 a         2273 c         1873 g         4818 t
ORIGIN      
        1 atagttacgt ttttggaatt aacgttgaca tattgtcatt cagttttcaa tgttcgtttg
       61 tcacttacaa gatttaatta taacgcgtaa gtttttgtaa gtcaagaatt atttttaatt
      121 attttatttc taaaaaataa atttcctaac tcaattaatt caacaaatat tattttatta
      181 tgtatttgtt cgaatttcaa tagtcaaatt tacactttat taatatttta aatcaacaac
      241 ttaaaacatt tgttttttaa atattagtgc cgtttgacga cttttatata atatcaagtt
      301 attctagtag agtcaacata aaacaacact tatttttgtt taaaaattac tgttgaaggg
      361 attattttct attcgtaagt actatatgta tttaaagtgt aattccttac gctttttact
      421 tcagttttac aaacatactt atcctcactt tatggatagt tatttagtaa accttcgttt
      481 gaatattttt atgtttattt ataaaggata ctagttatta gttaatacca cttccgtagt
      541 aagaatgttc ttttcttcta tttaaatatt tttcagaata agcataaaaa agaataagca
      601 taaaaaagaa taagcataaa aaagaagtta aacaactttc atgtcgttta acttcttaaa
      661 tattacgctt acttatatga agtaagctaa cgccttttta atttgtcata ctaccaaagt
      721 taaattattt atcttggcat tctttacaaa ctccataaat ttccattcga tgatgtgtta
      781 cgtcaaagtc agtcatatgc tgagctaatc tttcaatttc atttaactgt ggatattgaa
      841 aatcaacaat cttaccacat tgttcacata taatatgata atgattatgt gtattaaagt
      901 cgaatcgact tgatgagtct ccatatgtta attcttttac aattccaata tctttaaaca
      961 ctcttaagtt attatatatt gtcgcaacac ttatatttgg aaaatcaggt gaaagtgctt
     1021 gataaatttc atcagctgtt ggatgagtat gtgaagaaat taaataacgt aatattgctt
     1081 gtctttgagg tgtaattctt acgcctgctt gtcgcaatga tgcaattgat tcttctagtt
     1141 catgttcaat tgattctatt tcaacactca tctatatcac catctttctt atataataat
     1201 tattattact tactataaat ataactgtag tattgcgcgc gtgtcaatat ttacatgatg
     1261 atttcattta atagcctttt ttagcatcaa cttcattctc aattagacca ttcttattga
     1321 gaaaattaac tagattgttt ttaaaaatat ctaataagtc atactttgct tcataatcat
     1381 taccagttat atgcgctgtt atagttacat tatccaattc atataattca tgattaggtt
     1441 tcaaaggttc attttcaaac acatctaaat atgcatgtcg aataactcta ctttttaata
     1501 cttctattaa gagcgcttct ttaactatgc tacctcgtcc tatatttata aaaagtgctt
     1561 catctttcat taattcaaaa tgttttttct ttagtaaatg aatcgtttct tgcgtttctg
     1621 gtaaagcatt tataacaatg tcagcatttg gtaatgtgct ttcaagtgat tcgatagtat
     1681 atatctcatc aaactcatct ttgttctgac ctgacttgct cagaccaatt aaattcatat
     1741 taaaagcctt tgctaacttc gcagttctag tagcaattgc acctgtacct aaaaataaaa
     1801 ctgtttgtcc tgataggcgt ttaccagtta ttttcgaatc atatatatgt tgtcgttggt
     1861 tatcatatga tagtttcatc tttttataat catctaaaat gaaagccaaa atgtattcag
     1921 ataattgttt agcttgaaca ccttttccat tagttaaaag tatgccgtga tctgcaatat
     1981 aatctaacgg taatgtattt acacccgttg caaaccatgc gatccatttt aaatttggac
     2041 aacgtcgtaa aaatgcctca ttgataccgc catcataacc aactaatata tctaaattag
     2101 ccaagtcgct ctcaggtatt tctgatgctt ttttataaaa tttaaaatct acatctgaaa
     2161 agcgttgttg taattcagtt tcaacttcac gcatacgatt caacccaaca actttcatat
     2221 atgtcacccc aaaatgtttt taagttcttc tatttgtgtt ttaactttaa ccttctcgat
     2281 aacatctaat actttacctt gttcatctat tataaaagtc gttcttacaa tgcccatact
     2341 ttctttgcca aatgattttt ttaactgata tacgccagtt tctttagcta atttaaaatc
     2401 ttcatctact aataaatcga aattcaatcc gtatttttca ataaaatttt ggtgtttttt
     2461 ctttgaatca ccgcttatac catatactgc aacatctaaa tcattgaaca tttctaaatt
     2521 gtctctaaag tcacaagctt ctgtggtgca agtaggtgta ttatctctag gataaaaata
     2581 tacaatcgcc tttttacctt ttaatgtatc atttgtaatg acagttccgt cctgattttc
     2641 taatttaaat attggaaatt gttctccttt ttgcaacata taatcacctt tttcttcatt
     2701 ataataattt tatgatacga tattagatga aaatattaaa ttaaaagagg ttgatataaa
     2761 tgaattttag tgaaagtgaa cgtttacaac aactttcaaa cgaatatatt ctaggcggtg
     2821 tcaattcccc ttctcgttct tataaagctg taggaggcga tgcacctgtt gttatgaaag
     2881 aaggacacgg tgcatattta tatgatgtcg atggcaataa atttattgat tatcttcaag
     2941 catacggtcc aattattacg gggcatgcac atcctcatat tactaaagca attcaagaac
     3001 aagctgctaa aggtgtttta tttggtacac cgactgaact agaaattgaa ttcagcaaaa
     3061 aattacgtga tgcaattcca tctcttgaga aaattcgctt tgtaaattct ggaacagaag
     3121 cagtcatgac aacaattcgt gttgcacgtg catatactaa aagaaataaa attataaaat
     3181 ttgctggatc ttatcatggc cattctgatt tagtattggt tgcagcaggt agcggcccat
     3241 ctcaactcgg ttctccagat tcagctggtg ttccagaaag cgtagcacgt gaagtcatta
     3301 ctgttccttt caatgatatt aacgcatata aagaagcaat tgaattttgg ggtgatgaaa
     3361 ttgccgcagt attagtagaa ccaattgttg gtaactttgg aatggtaatg cctcaacctg
     3421 gatttttaga agaggttaat gaaatttcac atagcaacgg gacactagtg atttatgatg
     3481 aagtaattac tgcattccgt ttccattacg gtgccgctca agatttatta ggtgttatcc
     3541 ctgatttaac tgcatttggt aaaattgttg gcggtggttt accaattgga ggctatggtg
     3601 gacgtcaaga tattatggaa caagtagcac ctctaggacc tgcatatcaa gctggtacaa
     3661 tggctggtaa tccgttatct atgaaagcag gtattgcatt actcgaagta ctagaacaag
     3721 acggtgttta tgaaaaatta gacagcttag gccaacaact agaagaaggt ttacttaaat
     3781 taatcgaaaa acataatatc acagctacaa ttaatcgtat ttacggatct ttaacattgt
     3841 actttacaga tgaaaaagtc acacattatg atcaagttga acattctgac ggcgaagcgt
     3901 tcggtaaatt tttcaaattg atgttaaatc aaggtatcaa tttagcacct tctaagtttg
     3961 aagcttggtt cttaacaact gaacatacag aagaagatat taaacaaact ttaaaagctg
     4021 cagactatgc ttttagtcaa atgaaataac ttttaaggaa atatatttta aattgaatta
     4081 aaataaaaca aatgctcaca tagtagattc attttatatc gttaactatt aacgtttaaa
     4141 ttgatatgtc cctatgtgag tttttatttt acaaatttat actattttaa tctctgacta
     4201 aagttttaca tcgctatcag tattatgtat gatttatttt aacaactata aataagcttg
     4261 aattttgtaa ctagtaagtg tataacagta atgaatacga atgttagata ttcaataaac
     4321 ttaaaagggg cgtcattatt tttgagacta ggagctcgga ttttcaaaac tggtatagcc
     4381 attattttag ctatgtctat cgcttcttta ctaccggatg atgttggtct gaaagcctta
     4441 gctggtgtca gtgctgttgt tgcaatgcaa cctagtattt atcgttcatt taaaacagtt
     4501 tctgatcaag cattgggtaa cattataggt gccatactat ctgttacaat ggtaacaatt
     4561 tttagtgata attttatcat tatgggtgtt accgtcatag tacttattgc tattttattt
     4621 aaatttaatt tagcacatgt tgctactttg gcgagtgtaa cagcattaat cattatggga
     4681 caacataccg gttctttcta tattactgcc ttttacaggt ttgtacttgt aatgattggt
     4741 gtcataagtt catctctagt taactttgtg tttctaccac ctaaatttga aacaaaaatt
     4801 tactataatt cattaaatat atcctcagat atttttatgt ggtttaaatt agtattaaat
     4861 gatacgacgg agtttaataa tattaaacaa gatagtcata atttaaaaca acgtgtcgaa
     4921 aagttagaga aaatttatga ttactatagc gaagaacgtc ctattacaaa aaaacatatt
     4981 caccaacaga atagaaagaa aatacttttc agagaagtag ttcagacgac tagacaagct
     5041 tatgaagtat taaacaaatt gtcgcgttat caaaacgact tatatttact taataacaat
     5101 ttcctattac aaatcaaatt agatttagat tcattaactg cttttcatga gcaaatttta
     5161 gctagccttt ctaaaaaagc acgttataat gtgacacatg ttgattatga gttagataat
     5221 ccgcagaaaa aagatttatt gtctacattt cagcatgagt taataaatca cccttatcaa
     5281 acggaatatt cgtttgccaa tgttatgcaa attgttgcag ccatcgaaga gtatcgacac
     5341 catttagagc atttagaccg cataagaatt agctttttca catatcatag atctgatgca
     5401 gacattgaaa ttgttgaaga agactttgat ttataacatt aaaacaattc ctgtaattaa
     5461 taccacaatg agataaagta atattgaatc tcgaaattaa atttaaaaaa gcagtaagat
     5521 tattttcaat gaagaaaatc tcttactgct ttttctatgt tatgacctca ccatcctgtt
     5581 acaaatcttc cctacaaaat tgtactgaag tcaaacaact aatactttat aaattttgaa
     5641 tgctatataa atgctcgtaa gcaccttgtt ttgcaatcaa ttcacgatgc gtacctgttt
     5701 caacaatatg tccattttca attacgacaa ttttgtcagc atgtgtaata gtggacaagc
     5761 gatgcgctac gataagtgtc gttcgatctt tactcaacac atctaatgct tcttgaataa
     5821 tggattcact ttctaaatca agtgcacttg ttgcttcatc caggataaga attggcggat
     5881 tatttaaaaa tattctagca atcgataatc tttgtttttg accacctgat aatttaacac
     5941 ctcgttcacc tacttcagtg tcatatccct gtggcaagtt catgataaag tcatgtgcat
     6001 tagccatttt cgccgcttca actacttctt cctctgttgc tgttggacga ccaagtaaaa
     6061 tattttcctt aactgtatca gaaaataaaa tattatcctg ttgcaccaat cctatttgat
     6121 ttcttaaact tcccgttaaa aaatctttaa tgttgtgacc atctattaaa atttgcccag
     6181 aagttacatc gtaaaatctc ggtattaagt taattaatgt tgatttacca ccaccactca
     6241 tacctacgaa agcaactgtt tctccttttt caatactcaa attaatatct tttaaaattg
     6301 gagcttcgtt atcgttatat tgaaaactaa catgatcaat atcaatacga ccttgtttaa
     6361 tttcaatagg ttgagcacca acaccatttt taatgtcata atcttcgtca attaattgga
     6421 atacacggtc cattgaagca aaactttgcg ttaaagttgt aaatgatgcg actaaacgac
     6481 gtaaaggccc gaacaataac tccaagtatc caacaaatgc tgcaagtgta cctactgtga
     6541 ttgatccaga aatagcaaga tatgcaccaa caccgatgac aattattggt ccaatatctg
     6601 taactgtatt aattgcggca aaagaatagg cattccatct tgtatgtttc aacgcacgtg
     6661 ttaagaaatt agtgttcttt ttatcaaagt ttttcgcttc attgtcttca atcgcaaaac
     6721 ttttaacgac tgaaatacct tgaacacgtt catgcaagaa tccttgaacc tcagctaatg
     6781 cttgagatct ttcacgcgtc aattttctta atcttccaaa gaaaacgtac accgttaaaa
     6841 tgtaaaatgg gaagataaac agtgctgcta aagtcaattt cacatctaaa aagaacatta
     6901 tggatagtgc aataataatt gttatacaat ctaaccaaat attcattaac ccggttaaaa
     6961 tgaaatcttt tgtttgttca acatcattaa tcactctaga tattacttga cctacttgat
     7021 tattagcata aaatctcgca cttaaagctt gtaaatggtt gtataacttt ttacgtatat
     7081 catacaatat tttattactt gtccattgcg ccaaatattg acgtataaat tcaattggtg
     7141 gtctcactat tacaaaaata aataatgcga taccaatggc aatagttaaa tgatgaactt
     7201 tttcatcagt cgttaatgcg tggttattaa tcacgccatc tattgcatat ttaattaaaa
     7261 gtggtataag cattggtata ccaaacttaa ttatcccaac aataatcgtt gcaaaaatac
     7321 gatatttgta tggcttaaca aattgcaaat atcgtttaat catacaatcc cccctaatct
     7381 attgccctat cctattcata agcataaaaa tgaatagagg ttggatacat aatttgtaga
     7441 tgtaaattct tcttacaatt tacattttta aaattaagat atctcaacct ctgtcatatt
     7501 catgtgatta aatacgttgt cttattcgta tgttatattc tattcaattt aatctatgga
     7561 tactgtgtcc ccacacgaca gcaaaagtta tcatacttct ttacatcact aagtcaatat
     7621 aaatgattta atcagtattt acactttatt tgcttaatat tgtctaattt ttttgtaacg
     7681 gtctttccaa actttgataa aatctggcgc aaatgggccc ttcttttgtt ctatccattg
     7741 ttgaagaatg tccacgttgc gtcttaaaat aatatcaata tcatgcggat aattcatttg
     7801 attcatatgt tgctcatatt catcttcatc taataaatga tactttccat ttggatatac
     7861 tttaatatct aaatcatagt ctatatattt taatgcctct tcatcacaaa caaatggcga
     7921 tgacaaattg caatagtaat aaattccatc ttctctaaac atgcagataa cattaaacca
     7981 atattctgag tgaaagtaaa caattgccgg ttcacgtgtt atccaagttc ttccgtcact
     8041 ttcagttact aacgtatgat cattgccacc aatgacaaca tgatcagtac cctttaatat
     8101 tgttgtttca gaccaaacgc gatgaatctt accatcatgt ttataactct gaattttaat
     8161 gttttcccct tctttaggta tggattctct gaccatactc cacaccacct tctgttaatt
     8221 taaccattat aaattatagc atatttcaga aatagtatta tataaataca tatttttacg
     8281 aaataagatt ttactactta atgattaaac tcggtaatat tgctaagtac tacaacagag
     8341 atttacatgt cccatttaaa gtatataaaa tcatcacttt tatatatcaa cactttaact
     8401 ttttgacatt gttatcctct gagaattgaa gataacattt atacattttt aaaattaatg
     8461 tcactatatt ttccgataat cttatcaatc atcgaatgtt acccatttat aaattgataa
     8521 atctttgaca taggtacagg gaatgtatat tgatctcgat cacttaaatc aaaccaaatc
     8581 atgtcatctg gtaatctttc aatgttaatt gttcctgaaa cggcgtatac ctttatcttc
     8641 catgttaaat gagtaaattg atgcttcaat tcaaaaatag gtgtttctac tggttgaatg
     8701 tcatgaccga ttttttcagt catttcacgt ctagcatgct cacttttaaa cataggaaat
     8761 tgccacatac catgcaataa tttttcgcta cgcttttgca acagatattg accttgatta
     8821 tttctaatta aaaagacgga ttgctcaatt acttttttac ttacattttt agatttaact
     8881 ggtaactttt caaatgtacc tttatcaaat gcctcacagt tttcttgaac tggacaaaat
     8941 aagcataatg gatttttcgg tgtacaaatt aatgccccta attccatcat agcttgatta
     9001 aacgttccag cttctgtagt aacatacggt aataattctt gttcgtacga tttcctcgtc
     9061 gattgtaatt taatatctcg atagtcatca ttcaatctag accatacccg aaaaacattt
     9121 ccgtctacag ttgctagtgg tacattatat gcaatgctca ttactgcagc ttgtgtgtat
     9181 gggccaacac cttttaaagc tttaaattga tcaggatctt tgggaactaa gccttcatat
     9241 ttatcatgaa cttccttaat tgccgtatga aaatttcgag ctctactata atatcctaag
     9301 ccttcccagt actttaacac ttcatcttcc gaagcttgac tcaaaacttc cacagttgga
     9361 aatcgttcaa caaaacgatg ataatagtca ataactgttt taacttgtgt ctgttgtaac
     9421 atgacttcac ttaaccaaat atagtacgga ttggtcgttt gtcgccatgg catttctctt
     9481 tgattttcat caaaccagtg tatcaaattt tctttaaaac tagactgctg atacatttat
     9541 aaaacccttt cctcaccaaa attaattgtc tttactcata atgtttttat tgtacattaa
     9601 aatcatggtt agtatgtaag ttaatttagt tatttgcgaa attggattat aatagtatat
     9661 ataatattat gaaatgagtg aactgatatg gacactgcaa cacatatcgc aattggggtg
     9721 ggccttacag cacttgcaac tcaagatcca gcaatggctt ctacgtttgg tgcaacagct
     9781 acaaccctta tcgttggttc attaattcct gatggggata ctgttcttaa attaaaggac
     9841 aatgcaacat atatttcgca tcatagaggt atcacgcatt ccatcccttt cacaatacta
     9901 tggccaattt taattacatt tttaatattc acgttcttta gtggaaccca cccatttcat
     9961 gtatggatgt gggctcagct cgcagtattt ttacatgtct ttgtagatat attcaattct
    10021 tatggtacac aagctcttag acctatcaca aacaaatgga ttcaattaag tgtgattaac
    10081 acatttgacc ctattatttt cacagttctt tgtattggta ttgtattatg ggttgtaggc
    10141 ttgcatccgc ttgcagtctt ctttcctata atcgctttac taatcattta ttacatgatt
    10201 cgttttaaaa tgagagccgt aattaagcaa caagctttaa aagcaattca acaagagcat
    10261 caccctgtta aagtatttgt tgcgccaaca ataaaattta tggaatggcg tgtcgcgata
    10321 caaactgatg cacatgacta tgttggaaaa gcatatggta gaaatgtggt gtttagtgat
    10381 aaagttgaac gtcaaaaatt atcaacagac tccattttat ggaaagtcaa aggtaataaa
    10441 gatatacgta cttttttaaa cttttcatca atctatcgtt ggcaaacaac aacgttagca
    10501 gatggttcta ctgaaattcg tttgattgat ttgcgttatt taaaaaatga tcattattca
    10561 tttgtggcaa ttgcacacgt aacaaaagat aatgtcatag accactctta tattggctgg
    10621 gtatttacag aagataagtt acaacgtaaa ctgtatgcta aataatttca agttattatt
    10681 cactaaagtt aatctataaa aaatgaacaa ccgggcagaa tgaaaatcaa aacgattttt
    10741 actctgtccg gttttttaat gtaaaactat gaatgctttt acaaaaccta atatttatat
    10801 tgtcgctaac aaactacctt caatgactcg aaatattaaa atcagtatag gaaaacaata
    10861 tctagattat attctaatta tttctgattc tcacagatta atttacacaa caagtcagct
    10921 aaacatcatg aagaagtatc cgcctcgtct gtactatctt ttgaaacatc ttgttgatta
    10981 tcagtttgtg tcactttaga tgttttatta taaattgcgt gtgtcgtata ccttgctaat
    11041 acgaaattta ctgcagctat taaacataag aataaaataa agtaaaaatg atatggaatg
    11101 ttttccatac ttgatatacc aaatatgata gattgtgcta taccattaac aatgtaatac
    11161 attggattca gcattaggat gtgattgata aatacatgat ttggatttgg tataaaaata
    11221 attggcaaca ctaagaaaca caatacacaa atcccataaa atatgatatt tattttttca
    11281 gttaacagtc gaataagacc aaaagtaact gatattaatc ctacaaaaat agttgccatc
    11341 acaataaaat agaatagcgc tatatatgat gtttcgaagt ttactggttt aaccaatgca
    11401 ctaatcatcg tcaaaatgac tagcataata aaacttaaaa tagacataat aactactggc
    11461 gtcgctgcgg aaatattaaa taacttagta actaaaaagt cattcatata atgatgataa
    11521 gagatataaa ttgtgaacat caataccgca aaaacaatca atgcaataag tctaaataac
    11581 catcgcacct ctttaacatc ggtcaaatga ctaaaggaaa atagcgtttc acttgcaata
    11641 ataagtatta aacatgcagc taatacgatt agaaaacttt tccaactttg ttttaaacgt
    11701 tctgttacat accttttagt atgcggtaag tttttaaaat ataaaataat attatctagc
    11761 atctttacac ctacaattca atataaatcc atttgtcttc ttttaaattg gcaatacaat
    11821 atccattccc aactttagta atccaaatgt tattgttaat attaaactta tcttttaatt
    11881 cttttttatc tacgattgga taaacaaaac cgtataactg attattatca ttgaaaagta
    11941 attgtatcgg ttgttgtgta acggatgcaa cgaaacggtt atcatcatca ggaactaaag
    12001 ttttaataac tgaactatgc tttttatgta attgactgtt aaagtatgca tagtaattac
    12061 tataatttga tttcataaat ggcgctaatt ttttaaaact atgcgtttga tataaacctg
    12121 ccgggttaaa atattctaat ttatctttac taacatagta agtcttatta tcagcggtaa
    12181 cacgataatt tttactattt tcaccagtaa ttgtaactac actatatttt ggaattgtga
    12241 cttgtttatc cccttgcaga tccacactgc cattaaaagc aattccataa gctaactttt
    12301 cgccatatgg atctttattt tcattttgca tcgtagcacg attcgtaaaa tctgttatcg
    12361 aaataattcc tatattattg aaaatgagca acatcatcaa tgctaagcct aaaatagtac
    12421 ctgaggctaa agtccaaaaa cgcactaaaa acttaggcgg ttttgcatga ttatagcgct
    12481 caacgcgttt gaaattatag gtcatctccg gtattcttgt acgattcttt ttccaatcta
    12541 aatcaaagtt ttcaatttct tcttttgagt ttagacttaa tcgatcacgt tcatgttctt
    12601 taaaagatgg aataacttgt ttaagtgacc cttccattct taattgacca tgtgaaaacc
    12661 aagctatata gttactcact tgtgaaattt tatcaatatc atcaccaatt gacacaatcg
    12721 ttaaattatt ttcaatataa tcatttgtta attcaatcgc acgttccata aattgtggtg
    12781 ttaaatagtc aataacatga tttaaaataa taatatttga ttttgatgag cgtgcaatac
    12841 ttaatagtaa ttgagcgtat gccgctttcg aaatatggtt aaccggcttc gttttataat
    12901 cgcctaaatg tgcatattga ataatctgtt cagctttatg attattaatt tcatatggaa
    12961 atagttgaac taactgcgct gtataagctt caacagtttg atgaattaac gattgatctt
    13021 caatatatcc gtagaacaaa tcttcagtac aaactacttt acctttatcg ggtttaattg
    13081 cacctgccaa caattgacct accatcgctt tggaagattc aggttcacca attataccta
    13141 atgcttcacc ttgataaata tgtaaactaa tattgtttaa atcgatatct tgagcatcat
    13201 atccaaaagg taaataccat tttttattct gtttattcct atagtagtgt gttactttta
    13261 gtaactttaa aacaattgaa cttcccatct attttcatcc ttctataatt gtaataacgc
    13321 tataggtaag ccttcttcgg gttcatcact attaaatcta aacccccacg caaaaatacc
    13381 ttttaatctt tctactttaa aatattgatt actgccatca gtcag
//
LOCUS       DAMZPP010000079        13340 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMZPP010000079 DAMZPP010000000
VERSION     DAMZPP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13340
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.079"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..1043
                     /locus_tag="POH73_001718"
     CDS             <1..1043
                     /locus_tag="POH73_001718"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDI6887663.1"
                     /translation="SIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSG
                     VTFVRDLYEDLQRRDFTMNAIAMDTANKLYDYFDGQQDINNRIIRTVGIAEERFQEDA
                     LRMIRCLRFQSQLSFDIATETFEAMRTQMADIKFLSIERIVIELTKLMRGINVEKSFN
                     HLKSLKAFNYMPYFEHLDMNQINVTEPIDLELLIAIVSVKFDINYSLKPLKLSNRQVK
                     DINQYIQIMNALPSIITKEQLKMFVYDYDTNLIKNVMVAADVLKANDIQGHEPLIVNL
                     QTIDETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDLLRQIEIAIVTGKVSNEET
                     EILKWVDNHVKI"
     gene            1030..2001
                     /locus_tag="POH73_001719"
     CDS             1030..2001
                     /locus_tag="POH73_001719"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDI6887664.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     DFDESIRYRATSIQLHDKNKLDRYQFLERLLQEIEKRYNQFLTLPFSEIREEYIAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            2025..4718
                     /locus_tag="POH73_001720"
     CDS             2025..4718
                     /locus_tag="POH73_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDI6887665.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAEEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAVLMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFDAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            5040..6332
                     /gene="asnS"
                     /locus_tag="POH73_001721"
     CDS             5040..6332
                     /gene="asnS"
                     /locus_tag="POH73_001721"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDI6887666.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKMLERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            6661..7347
                     /locus_tag="POH73_001722"
     CDS             6661..7347
                     /locus_tag="POH73_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HDI6887667.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKEQNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYMDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            7337..7996
                     /gene="nth"
                     /locus_tag="POH73_001723"
     CDS             7337..7996
                     /gene="nth"
                     /locus_tag="POH73_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDI6887668.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            8001..8342
                     /locus_tag="POH73_001724"
     CDS             8001..8342
                     /locus_tag="POH73_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887669.1"
                     /translation="MTNITKEVFDNLEQKIDVFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDNLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(8892..11042)
                     /locus_tag="POH73_001725"
     CDS             complement(8892..11042)
                     /locus_tag="POH73_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDI6887670.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTAEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQS"
     gene            complement(11039..11716)
                     /gene="recU"
                     /locus_tag="POH73_001726"
     CDS             complement(11039..11716)
                     /gene="recU"
                     /locus_tag="POH73_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDI6887671.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNAFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEIFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
     gene            12204..12554
                     /locus_tag="POH73_001727"
     CDS             12204..12554
                     /locus_tag="POH73_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HDI6887672.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     LLNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTYG"
     gene            12547..13110
                     /locus_tag="POH73_001728"
     CDS             12547..13110
                     /locus_tag="POH73_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDI6887673.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            13124..>13340
                     /locus_tag="POH73_001729"
     CDS             13124..>13340
                     /locus_tag="POH73_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDI6887674.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLR"
BASE COUNT         4804 a         1871 c         2306 g         4359 t
ORIGIN      
        1 aatctatctt tagtcatacg atacctgtag gtaaagaaca tggcaccata aatgtagttt
       61 ttaatgatga aaattatgag gtgacaacat tccgggctga agaagattat gtcgatcacc
      121 gtagaccaag tggtgttaca tttgttcgtg atttatacga agatttgcaa cgaagagatt
      181 tcacgatgaa tgcgatagca atggatacag caaacaaatt gtatgattat tttgatggtc
      241 aacaagatat taataatcga ataataagaa ctgtaggtat agctgaggaa cgattccaag
      301 aagatgcttt acgtatgatt cgatgtttaa gattccagtc acaattatca tttgatattg
      361 caacggaaac attcgaagcg atgcgtacac aaatggcaga tattaaattt ttatcaattg
      421 agcgtatagt gattgaacta actaaattaa tgcgaggtat taatgttgaa aagagtttta
      481 atcatttaaa atcgctgaaa gcatttaatt atatgccata tttcgaacat cttgatatga
      541 atcaaattaa tgtgactgaa ccaattgatt tagaattgtt gattgctata gtatcagtta
      601 aatttgatat taattattca ttgaagcctt taaagctaag taaccgacaa gtaaaagata
      661 tcaatcaata tattcaaatt atgaatgcat taccaagtat tattacaaaa gaacaattaa
      721 aaatgtttgt ttatgattat gatacgaatc tcattaaaaa tgtaatggtt gcagcagacg
      781 tgttaaaggc aaatgatatt caaggacatg aaccattaat cgttaacctt caaacgattg
      841 atgaaacatt acatcgttta cctatgcata atagaaaaga catgatggtt aatggcggtg
      901 tacttatggc acatttaaat gctaaaagtg gtccgtggtt aaaagatctg ctaagacaaa
      961 ttgagatagc gattgtaact ggtaaagtaa gcaacgaaga aactgaaatt ttgaaatggg
     1021 tggataatca tgtcaaaata tagtcaagat gtacttcaat tactctataa aaataaaccg
     1081 aattatatat ctggacaaag cattgcggaa tcacttaata tatcgcgcac tgcagtaaaa
     1141 aaagtgattg accaattaaa gttagaggga tgtaaaatag attcagtaaa tcataaagga
     1201 catttattac aacaactccc agatatttgg tatcaaggta taatagacca atacacaaaa
     1261 agttctgctt tgtttgattt tagtgaagta tacgattcaa tagattctac acaacttgct
     1321 gcgaaaaagt cacttgttgg aaatcaatct tcatttttta tcttgagtga tgaacaaacg
     1381 aaaggtcgcg ggcgatttaa tagacattgg agttcttcaa aagggcaagg actatggatg
     1441 tctgttgtat taagacctaa cgttgcattc tcaatgatat ctaaatttaa tttatttatt
     1501 gcattaggga taagagatgc gattcaacat tttagtcaag atgaagtcaa agtgaaatgg
     1561 ccgaatgata tatatattga taatggtaaa gtgtgtggtt tcttaactga aatggttgct
     1621 aataatgatg gtatagaagc aataatatgt ggtataggta ttaatttgac gcaacaacta
     1681 gaagactttg atgaaagtat tagatataga gcaacaagta tacaattaca tgataaaaat
     1741 aaattagata gatatcaatt tttagagaga ttacttcaag aaattgaaaa aagatataat
     1801 caatttttaa cgttaccttt ttctgaaatt cgtgaagaat atattgcagc ttctaatatc
     1861 tggaatagaa cgttgctatt tacagaaaat gataaacagt ttaaaggaca agcaattgat
     1921 ttagattacg atggctatct aattgttaga gatgaagcgg gtgaatcaca ccgtttaatt
     1981 agtgcagata tagattttta acactaaagc aaggagagat agctatgggt atggcaacct
     2041 atgccgttgt ggatttggaa acaacaggca accaattaga ttttgacgat atcattcaaa
     2101 ttggtataac gtttgtgcgc aataaccaaa ttattgatac atatcattct atgattagaa
     2161 caaatttaga gattccgcct ttcattcaag cgttaacatc cattgaagaa aatatgctac
     2221 aacaagcgcc atatttcaac caagttgcag aagaaatcta cgataagata aaagattgca
     2281 tttttgtcgc tcataatgta gattttgatt taaattttat taaaaaggca tttaaagatt
     2341 gtaatattca atatcgtccc aaaaaagtta tagatacact agaaatcttt aaaatagctt
     2401 ttcctacaga taaaagttat caattaagtg aattagcgga ggcacatggt attaccttag
     2461 caaatgccca tcgagctgac gaagatgccg ctactactgc taaattgatg atattagctt
     2521 ttgaaaaatt tgaaaagtta ccgttagata ctttaaagca attatattat ttaagtaaac
     2581 agttgaaata cgatttgtat gatattttct ttgagatggt taggcaatat gatgcaaagc
     2641 cattggataa atcgtacgaa aagttcgaac agataatata tcgtaagcaa gttgatttta
     2701 aaaagccgac aacaaattat aatggcagct tgaagtcttt atatagcaaa gctgtagatc
     2761 aacttggctt aacatatcga ccgcaacaat tatatttagc ggaaactata ttagatcagc
     2821 tcatgcatag tgaaaaagca atgattgaag catcactagg cagtggtaaa tcattagcat
     2881 atttacttgc ggtcttgatg tataatattg aaactgggaa gcatgtcatg atttctacca
     2941 atactaaatt actgcaaagt caattgttag aaaaagatat tccagcaatg aatgaagcgt
     3001 tgaattttaa aattaatgca ctattgatta aaagtaaaag tgattacatt tcattgggac
     3061 ttattagtca aattttaaaa gatgacacaa gtaattacga agtgaatatc ttgaaaatgc
     3121 aattgctgat ttggattaca gagacgccgt ccggagatat acaggaatta aatttaaaag
     3181 gtggtcaaaa gatgtatttt gaccagaaaa ttgaaacata tgtaccggca cgtcacgatg
     3241 ttcattatta taattttatt aagcgaaatg cacagaacat tcaaattggt attacaaatc
     3301 atgcacattt aattcattct gatgttgaaa attcaatata tcaattgttt gatgattgta
     3361 tagtggatga agcacatcgc ttaccagact atgccttaaa tcaagtgaca aatgagttga
     3421 gttatgctga tattaaatat cagcttggtt tgattggtaa aaacgaaaat gaaaaattgt
     3481 taaaggcaat tgatcaactg gaaaagcagc ggattttgga aaaattagat attgcaccaa
     3541 ttgatatttt tggcttgaag gctagtatga atgagattca tgagttaaat gagcaattgt
     3601 tctcaacgat atttacgatt atcaatgatt ctgatgttta tgatgatgat attcatcgct
     3661 tccataatgt atttactttt gaaacaaaag acattttgaa ggatttacat gcgattatcg
     3721 ataagttaaa taagacgcta gaaatattca atggcattag tcataaaaca gttaaatcat
     3781 tacggaaaca gttgttgtat ttaaaagata aatttaaaaa tatcgaacaa agtttaaaag
     3841 ctggacatac tagtttcatt tcaattaaaa atctatctca aaaatcaaca atacgtttat
     3901 atgtgaaaga ttacgctgta aaagatgtat taacgaaaca agtattggaa aaatttaaat
     3961 cacttatttt tatatctggt actttaaaat ttaatcattc gtttgatgct ttcaaacagt
     4021 tattcaacaa agatgttcac tttaatacat ttgaagttaa cacatcgtta caaagtgcaa
     4081 aaaatacaag tgtatttata ccgagtgatg tagcatctta tcaatataaa aatatagatg
     4141 agtatgtagc atcgattgta agctatatta ttgaatatac gactataacg tcatcaaaat
     4201 gtttagtctt atttacgagt tataaaatga tgcatatggt acaagatatg ctaaatgaat
     4261 tgccagaatt tgaagattat gttgtattaa cacaacagca gaaccaaaat tataaaatag
     4321 tacaacagtt taataatttt gataaagcca tactgcttgg tacgtcaaca ttttttgaag
     4381 gctttgattt tcaagcaaat ggtattaaat gtgtgatgat agcgaagtta ccgtttatga
     4441 acaagcataa tgcaaaatat tggcttatgg attctgaatt cacttcaact ttcaaggaat
     4501 atgtattacc tgatgcagtt acacgtttta gacaaggact tggaagatta attagaaatg
     4561 aaaatgatcg cggcataata gtttcatttg atgatcgact cataaacagt aattataaaa
     4621 acttttttga acaaacactt gaaaattatc gtcagaaaaa aggcgacatt caacagtttg
     4681 gaaaattatt aagacaaatt caaaaaaaga aaaagtaaaa tacaaagtaa aattcttgtg
     4741 acatgacatg tgctatgttc tgttacaaga atttttgttt ggtcgtcaaa atttgagttt
     4801 tataattaaa cttgaatgtt agcacaaaaa acgaccttca tactaataat attgaggctt
     4861 tattatatat tttgtctgaa actgatgatt gtattgtctt aatataaatt aatgttcaaa
     4921 tctgtgctta aatcattacc atttacacgt tatcttttag tctagttcta gaacttgaaa
     4981 gcggattttg ttaaaatgaa tgagtattgt ataacgaaaa aataaatagg agaatggtta
     5041 tgaaaacaac gattaaacaa gcgaaagatc atttaaacca agacgttaca attggtgctt
     5101 ggctaacaaa taaacgttca agtggtaaaa tcgccttttt acaattacgt gatggaacag
     5161 gctttatgca aggcgtagta gttaaatcag aagttgatga agaggtattc aaacttgcga
     5221 aagaaattac tcaagaatca tctctatatg ttacaggcac aattacagaa gataatcgtt
     5281 ctgacttagg atacgaaatg caagtgaaat caattgaagt tatttcagaa gcgcatgact
     5341 atccgattac acctaaaaat catggtacag aattcttaat ggatcaccgt catttatggt
     5401 tacgttctaa aaaacaacat gctgtaatga aaattagaaa tgaagttatt cgtgcaacgt
     5461 atgaattttt caacaaagat ggatttacaa aggttgatcc accaattttg acagcaagtg
     5521 caccagaagg tacaagtgaa ttattccata ctaaatactt tgatcaagat gcgtttttat
     5581 ctcaaagtgg tcagttatac ttagaagctg cggcaatggc acacggaaaa gtattttcat
     5641 ttggtccaac tttcagagct gaaaaatcaa aaacacgtag acacttgatc gagttctgga
     5701 tgattgaagg ggaaatggct ttcacaaatc atgctgaaag tttagaaatt caagaacaat
     5761 atgtaacaca tgtagtaaaa tcagttttag aaaattgtaa actagagttg aaaatgttag
     5821 agcgtgatac atcaaaactt gaaaaagttg cgacaccatt ccctagaatt tcatatgatg
     5881 atgcaattga gttcttgaaa gcagaaggct ttgatgatat tgaatggggc gaagattttg
     5941 gtgcgccaca tgaaacagcc attgctaatc attatgattt accggtattt attactaatt
     6001 atccaactaa aattaagcct ttctatatgc aaccaaatcc tgagaatgaa gaaactgtat
     6061 tatgtgcaga cttaattgca cctgaaggat acggtgaaat tataggtggt tctgaacgtg
     6121 tggatgactt agaattgtta gaacaacgcg ttaaagaaca tggattagac gaagaagcat
     6181 atagttacta cttagactta cgtcgttatg gtagtgtacc acactgtgga tttggtttag
     6241 gtttagagcg tacagtcgca tggatttctg gtgttgaaca cgttcgtgaa acagcgccat
     6301 tcccaagatt attaaaccgt ttatatccat aagttttagg gtcgttactt aaaatttata
     6361 ttatgataat aaaaagaatc gtccagccta tattaatagc tgggcgattt atgcttggaa
     6421 aatgaagaat gataacggct tatattaatc atgaacaaac ttttgtttag taactatatt
     6481 tggaaaataa atagctcgtc tgattataca taaagttcat tgttaaaatc tagttttaaa
     6541 aaaatactgt tatttaaaat ttgtttgcta ctagttaaat atattaagca ttttagtcga
     6601 aaattaaaga aaatgattta tactattaag taatggttaa atttaaaagg gggcacgaac
     6661 atggataaat atcaattaaa agcaagacct gtagtgatac gaagagaatt attagaccat
     6721 tacagtgact taggtttaga tgagcaagat ttagtcattt tgcttaaatt gatttatgct
     6781 tccgaaacat caaacaaaca gccttcaatt gaattacttc agaagggctc aactatgcaa
     6841 ccacgtgata ttacaatggt tattcaaaat ttgattcaac gtgaattgct agaattacaa
     6901 gttcaaaaag atgaagaagg tagatttact gaatatatga atctagatcc ttttttcgaa
     6961 aaattaagtc atatattgaa gcaacaaagt atggaaacga aagagcaaaa tagtaaagaa
     7021 aaatttaaac aattatttag agttttagag gatacatttg caaggccgtt atctccatat
     7081 gaaatagaga cgttaaatca atggattgat gttgataaac acgatactgc aattatacaa
     7141 gcggcattag acgaggcgaa tagtttgaat aaacttagct ttaaatatat ggatcgtatt
     7201 ttacttaatt ggaagaaaaa caatgtcaaa acaattgacg attctagaaa aataagagaa
     7261 aaatttaata aaccgaaaat gacgcacaca gttaaaacgg tacctaaatt tgattggtta
     7321 aacggggaga accttgatgg taagtaagaa aaaagcatta gaaatgattg atgttatagc
     7381 aaatatgttt ccggatgcag aatgtgaact aaaacatgat aatccgttcg aattaactat
     7441 tgctgtatta ttgtcagcgc aatgtacaga cgttctagta aatagagtaa caactgagct
     7501 gtttaaaaaa tacaaaacgc ctgaagatta tttggcagtc agtgatgaag aacttatgaa
     7561 tgatattcgt tctatcggtc tgtatcgtaa caaagctaag aatattaaga aactttgtca
     7621 atctttgatt gatcaattta atggagaaat accacaaaca cataaggaat tagaaagttt
     7681 agcaggtgta ggacgtaaaa ctgctaatgt agtcatgagt gtagcatttg atgaaccttc
     7741 attagctgtt gatacgcatg tagaacgtgt ttctaaacgc ttaggtatta atcgttggaa
     7801 agataatgtg agacaggtag aagaccgttt atgttctgtc atacctagag atagatggaa
     7861 taggagccat catcaactca ttttctttgg aagataccac tgtttagccc gaaaacctaa
     7921 atgcgatatt tgtccattat tagaagattg tagagaagga caaaaacgtt ataaagctag
     7981 tttgaaagaa gcgtgataat atgacaaata ttacaaaaga ggtatttgat aatttagaac
     8041 aaaagattga tgtatttgcc aaaaataaga cattaggttc tagtgaagca aaaccttact
     8101 tggatgaata ccatagtaaa attattgatt attttaagca ggttaatgac ataactggca
     8161 atattgattt tgataattta aatcaatatc ctgttgtgcc aatgaatttt aaagaaagat
     8221 atgattatat gattgaacgt aaatatcatt ttatgggcta tcgacaaatg aagaccttta
     8281 aaactgaatt gattaaaatg aatgctagtt atcaaacaag gttaaagaat aagcaggtat
     8341 agacaagttt gatcatttcc taatgttgaa aagatgtaaa actaattcat gcacacatat
     8401 ttgtatttat gttaggtgtc atgtcattac aacgtcttaa tataaaaaat gtccatctac
     8461 atcataatgg atatcttgag gtatggttta agatatctat ttcgaatcgt aggtggattt
     8521 cttatatcta tgattgtgaa tatacgttgc aaacatgttg atatttcact gattaacgta
     8581 ctcagggaat tgataaacat atcaatttaa aacaacgtct aagatgacta tgagaaaata
     8641 taatcataat tttaaaaatg ggtacaacca cttttgcaat ttcaataatt ttacatagct
     8701 aaagatgtga atatgaaaga ctatttagat gtttcaaaat gtatgtgaat aactgatttt
     8761 acggtttcta ctaatatagc aatagcgtat gaatacatat aaaaaaacct cagctacacc
     8821 ataaatatgg tataactgag ttttttatgt tgagtggatt agttgaatat acctgttaat
     8881 ccaccgctgt ttcaagattg ttgagatcta gtattgttat ttgattgtgc agtaccactg
     8941 ctgtttgctg atgtgtcact accaccgtgt gtactacgat ttgtagtgtt gttatcttga
     9001 ctaccagaaa cattgattga tgggatacta ccacttactg agctaggacg tttaaagtct
     9061 tcgccatctc tagatgaaat ttttgacatc acattttcat ataagaactg tggatattct
     9121 tgttggctat gtcccacaaa tgagttttca ccatattgtt taactttact gaagcccatc
     9181 cacactgaca tagtgtattg aggtgtaaag ccgttaatcc acacgtcttt cgctgcatta
     9241 tcaggtaaat tatattgtga ataagtttca gcaccgtaag taccagtacc tgtcttagca
     9301 cccatattta ctccagatac accatggcca tatgcagaac catatggttt aaatgtacct
     9361 tttagcatct cagctaacat gtatgcagtg taatcactca tcgctttatg gctagtatga
     9421 tcgtattcga ttgtttcacc atcacgagta actacttttt gaattgaatg cgcgttgtta
     9481 taagtaccac cgttagcgat tgcagcaaat gctgatgcta attgtgttgg tgagaattct
     9541 gaagcagaac caccaagtac ttcagatgga ccaatatcgc cttcgtagtt taagccaagt
     9601 ttggcagcga atttcttagg tgcatcatta ccagcatttt gcttaactga ttgccaagct
     9661 tttaaagctg ggatattgaa actttgtcgt aaagcatcat aaatagatac agtaccgtga
     9721 ctcttcgtat catagtttct aaatgtagat ccatcaactt gatatgaaga ttcatcttga
     9781 atcgcatggt ttgttgccca tttcatattt tcaatggcag gtccatacgc taagaaaggt
     9841 tttaaagatg aaccagtagg gtgtggatct gttgcttggt ttctgttaac gacgtctttg
     9901 aaatcacgtc cgccagatat agcaactaaa ccaccagttt tactatcaag aatcgttgca
     9961 ccaacttgtt ggtctttatt cttgtagaag ctaccattat caacatcatt ttgtaatgtt
    10021 ttttgaacat ctttatccat gtttgtataa attttaatac cactttgtaa tacattacct
    10081 aaattttcat ctttgaatgc tttattattc attaattcag attttacaaa gttaacgtat
    10141 gaattatact cagaatcttg atttgtatca atgttttgac gttcttcagc agtacgattt
    10201 actaagttcg cttttaaatc gattttctta gcatcttccc actgtttatc tgtaatgcgt
    10261 ttatgataat gcattaagta taaaacagtg tttttacgat cttcagcagc ttttggatga
    10321 tcataaatat tatagttgtt tggaacctga ggtaaaccgg ctaaataagc ttcttccgct
    10381 aagtttaaat cttttaaatc tttattaaag taatacttag cagcagcttt aatacctgtt
    10441 acgccatcag aatagtaaat tttatttaga tatacttgga agatatcatc tttactatac
    10501 tcttgttcta aacgatatga taagtatgct tcttgagctt tacgtccaat agatttatgt
    10561 tgtgataaaa atgcatcttt aacaacttgt tgtgttaatg ttgaggcacc ttcagaacca
    10621 aatccaccag tcaagttctt accaattgca ccgaataaac gtttataatc aagtgcgcca
    10681 tgttcgtaga aacgattgtc ttcagttgca agtactgcgt ctttcattga tttcggcacg
    10741 tcttttaaat ttacatgctc atgtctttgg ccattatcta atgttttaac aagttctccg
    10801 ttcttgtcat atatctttgc aggaatcgga tcttgtaatt tagcttcggt aaaagcaggt
    10861 gctttccaag cataataagc aaacaataag atacctagta aaagaacaac aaaaaatgca
    10921 ataatcatga agccaataat cttaataatc gttctcttca catttctatt cttttttgag
    10981 ttggatttcc caccattatt accatttttc ttaggctgag aagatccttt gttttccgtc
    11041 atacgcggtc ctcactttca tctaatatca acttatcaac tgcttttaga tagtctaatc
    11101 ttggttgata ctgataagga atatggtaac cattttttcg tatttcatca actgttatag
    11161 actttttaat attatcttta tatctcttcc aaaatatttc gaattttgaa tagggtaaaa
    11221 gataaacttc atctagcgtt ttaaaacgaa tcattaaaaa cacaatacct ttttgttgat
    11281 atgcattttt catatgttcg acttgatggt catgaatatt atttaaagga aaggacgtct
    11341 tgtttttagt ttcctttgct tcaaaatcaa tataataacc ttgataaacg ccgttgtaat
    11401 cagttgttga aggtgtacga aaataagctt cgttaatcac agctttactc cgcttaggat
    11461 agttgacatt aactatttgt actggcgtag gctttttgtg aataactgca atgtcgcttt
    11521 taagataaaa cgcatttgaa tgttcgatat ctttttcaag tgacatacca cgtccaccat
    11581 actcaatatt actaaaggca gcggtctttt tccctccgtc tatagcacta tttttacgat
    11641 atggtttacc atttggataa ttcataaatt tcaccacaca agtttggtag taaacgttgt
    11701 ttattataca caacattata gtgaaatacc aaacttaaat tagtataaat gcgaaagaca
    11761 caatacacca aagcttattt taacgtgatt tcagcattaa cacaataata ttataataca
    11821 tttaccgtta atgttggaat tttacacaca attttaatta attgaaaagt tttgcttata
    11881 aaaatgaata ctcattacaa tatttttgaa ttttgttata aaagtcaaat catgatcagt
    11941 aattattatt tttgaggcaa gtacaagtat gtgttgttaa tcaacagagg tgtatttgat
    12001 ttaaatgatt ttattagatt aaaagtattt tcatcgtata acaacataca cgataaaatt
    12061 ttgtatttat ataggaagtt tttagattta cttgatttaa cagataacac atttgaaaat
    12121 aagttaaatc aaattatata gagtgttaaa acatgacgaa tacagtatat tgtgataaat
    12181 taaaaaatgt aggaggcgtc ctcatgaatg atgtagtcga atcactaatt tatgaagtta
    12241 acaacatgca acaaaatttc gaaaatgtga aatcacaaca acaagatcat gatttttacc
    12301 aaactgtaaa gccatatact gaacatattg atagcttgct caatgagatc aaattacatc
    12361 gtgaatttat tatagaagta ccttatatga attcaaggaa atttgagcta ttgattgcta
    12421 acattgaaca actttctgtc gaatgtcatt ttaagcgaac aagtcgaaag ttatttattg
    12481 aaaagcttaa aagtgttcaa tatgatttac aaaatatatt agatggcgta actaaagagg
    12541 gtacttatgg ttaaaacagt ttatgtaaca ggttacaaat cattcgaatt aaacattttt
    12601 aaagatgacg cacctgaagt acattattta aaacaattta taaaacataa aattgaacaa
    12661 ctgttggatg aaggattaga atgggtgtta atacaagggc aaatgggtat agaattatgg
    12721 actgcagaag ttgttattga attacaacga acatatgatt cgttaaagtt tgctgtaatt
    12781 acaccgttcc aaggacatac agaaaagtgg aatgaacata atcaaagtaa gtatgccaac
    12841 ataattaagc atgcagatta tgttgatagt atttttcata cgtcgtatca aggtcctttt
    12901 caatttaaac aagcagatca atttatgctt gaacattcgg atcaaacatt gctcatttat
    12961 gatgaggaac aagaggcgag tcctaagttc ttcaagcaga tgttagttga ttttatggat
    13021 aaaacaaact atacttgtga tattgtgacg ttcgatgaat taacagcatt catcaatgac
    13081 ttacagtggt ctgaagatca aagtttctaa tgaggtggaa aaaatgtcag atgtttcatt
    13141 gaaattatca gcaaaagata tttatgaaaa agattttgaa aaaacaatgg ctcgtggcta
    13201 tagaagagaa gaagtagatg catttttaga tgacattatt gctgattatc aaaaaatggc
    13261 cgatatgaat aatgaagttg taaaattatc agaagagaat cataaactta aaaaagaatt
    13321 agaagaatta agactacgtg
//
LOCUS       DAMZPP010000080        13063 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMZPP010000080 DAMZPP010000000
VERSION     DAMZPP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13063
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.080"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(8..991)
                     /locus_tag="POH73_001730"
     CDS             complement(8..991)
                     /locus_tag="POH73_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDI6887675.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            1128..1637
                     /gene="msrA"
                     /locus_tag="POH73_001731"
     CDS             1128..1637
                     /gene="msrA"
                     /locus_tag="POH73_001731"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDI6887676.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            complement(1840..4362)
                     /gene="mprF"
                     /locus_tag="POH73_001732"
     CDS             complement(1840..4362)
                     /gene="mprF"
                     /locus_tag="POH73_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDI6887677.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVVITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILN
                     KITWVRWVLYAVSLFLPLFIIYSMVRPPDKNNRYVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDVHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYLLLAIHTSACLLLLLNVVGIYK
                     QSRRAIIYAMISIILIIVATLFTYASYILITWLVIIFALLIVAFRRARRLKRPIRMRN
                     LVAMLLFSIFILYINHIFIAGTFYALDVYTIEMHTSVLKYYFWITILIIAIIVGAIAW
                     LFDYQFSKVRISSNIEECEEIIDQYGGNYLSHLIYSGDKQFFTNEDKNAFLMYRYKAS
                     SLVVLGDPIGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGQFNETYLSKAPIGVMRNENNEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(4843..6051)
                     /locus_tag="POH73_001733"
     CDS             complement(4843..6051)
                     /locus_tag="POH73_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI6887678.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
     gene            complement(6044..6211)
                     /locus_tag="POH73_001734"
     CDS             complement(6044..6211)
                     /locus_tag="POH73_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887679.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            complement(6186..7037)
                     /locus_tag="POH73_001735"
     CDS             complement(6186..7037)
                     /locus_tag="POH73_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDI6887680.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMTLNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
     gene            complement(7537..8997)
                     /locus_tag="POH73_001736"
     CDS             complement(7537..8997)
                     /locus_tag="POH73_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDI6887681.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCFVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
     gene            complement(9247..11649)
                     /gene="parC"
                     /locus_tag="POH73_001737"
     CDS             complement(9247..11649)
                     /gene="parC"
                     /locus_tag="POH73_001737"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDI6887682.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDSSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFISNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSYNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITIITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            complement(11649..>13063)
                     /locus_tag="POH73_001738"
     CDS             complement(11649..>13063)
                     /locus_tag="POH73_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDI6887683.1"
                     /translation="ESAFLLKNLKITLNDLRSGKERQEHYHYEEGIKEFVSYVNEGKE
                     VLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVRTKDGGTHEVGFKTAMTRVF
                     NDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELLQFEGQTKSKLGTSEARSAV
                     DSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKAREDARSGKKNKRKDTLLSG
                     KLTPAQSKNTDKNELYLVEGDSAGGSAKLGRDRKFQAILPLRGKVINTEKARLEDIFK
                     NEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDGAHIQVLLLTFFFKYMKPLV
                     QAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQKELGKGFTLQRYKGLGEMNP
                     EQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQPRREWIEKHVEFGMQEDQS
                     ILDNSEVQVLENDQFDEEEI"
BASE COUNT         4499 a         2372 c         1736 g         4456 t
ORIGIN      
        1 tttattttta atcttcatct aaaaagtctt taatagcttg tttatttgtg tttttattaa
       61 tctgtaatgc actaccatca gtatttgtat tgacatcttc gtatgagttc ttgattggca
      121 cagtcaatga cttaacatct ttttcacctc ggataccaaa actcaaacct gtttggaaga
      181 tccctgaatc aggaatgttt gtattcacat agcctcttaa aatacctgca acttttggta
      241 atttaacaac tgttctaaaa ttaaccattt cttttttcaa agtttgcatt acttgttgct
      301 gacgtcgcac gcgtccgaag tcaccttcag ggtcgtgacg gaatcttgca taaccaagta
      361 attctttacc attcaaccta tggttaccct ttttcaaaga tacaccaata tttttcgaca
      421 tatctttttc gacattaatt ggtacacctt ctggcattaa ttcatcaatc attttctcaa
      481 atccagtaaa atcaactact gcataatatt caggattaat tcctaaattt ttatcaagtg
      541 tttttctaag tagctctgga ccacctaaag cgtatgctga attaattttg tgttttccat
      601 accctggaat atctgcataa atatcacgca tgacagacat cattttcatc tttttattga
      661 taaagtcata ttgaacaacc atgatagaat ctgttcttga ttgtccacct tgtgctttat
      721 ctgcaccgag tacaagaata gaaattttac catcattttt tactggtcca ttaaattgat
      781 gtactttaac atctttcgca tgtttcttgg catattctac accgctattg taactatgta
      841 caatatatac aactaatgcg ataagtaaaa ttacaacaat cagaagaatg ataggtaatt
      901 tcctaatttt cttcttcttt tttcgcttag gcgccgaagt gcgatgttca ctttgacgtc
      961 tatattcgtt gtcattagtt tctttatcca tatttaccta ccttatatct tcaaaaatag
     1021 atttttgtgt actataataa tcacatatta acgttatata aaaacaattc aaaaagcaag
     1081 tgatttctta ataaatcgta ctaaggtcta atgaaaggat gtcaaaaatg aatattaata
     1141 cagcttattt tgccggaggt tgcttttggt gtatgacgaa accatttgac acctttgacg
     1201 gcatagaaaa agtaacttct ggatatatgg gcggacatat tgaaaaccct acttacgaac
     1261 aagtaaaatc aggtacgagt ggacatttag aaactgttga aattcaatat gatgttgcat
     1321 tattctcata caataagtta ttagaaatat ttttctcagt cattgaccca ttagatacag
     1381 gtggtcaata tcaagaccgt ggtcctcaat atcaaacagc tattttctac actaatgatc
     1441 atcaaaaaga actcgctgag acttatatcg agcagcttaa aaatacgatt aatgctgata
     1501 aggcaattgc aacaaaaata ttaccagcgt cacaatttta caaagccgaa gactatcacc
     1561 aagattttta taagaaaaat ccagagcgtt atgcagaaga acaaaaaata cgccaagaat
     1621 acaaaaataa acaataacat ttacacttga tgaaactgat tggactgata ataaaagtta
     1681 ggcgacttaa cttaagttca tttcaatatt aaaatacggt acagatagga aacttaaata
     1741 ttcttatctg tgccgttttt tataaatcat tctttataga aaccaaaaaa tccaagcgct
     1801 tcaggcataa ctgtatacct attagcactt ggattttaat tatttgtgac gtattacacg
     1861 cattacttta gaaagtgatt cccaaagcga attatcttta cgataaacta aaaagcgtgg
     1921 ttcccaattc ggattatatt tagatttata acgacgtaat ccttggaaac gatatagacc
     1981 gttgaaatgt tcaaagacac gacctgcaag tctttctctt aaatatgaat aatgcaattg
     2041 accaacgttc gataacgttg ccatacccat attaaatttt gtataacctt gttctttact
     2101 ccaaagtaac atgtgcaagt ataatccatc cattaacggt aaatctaact ctggcaacca
     2161 tctaattaaa tcgactgaaa tggcatcatt aaagtatgtt ggcattaaac tacaaaatgc
     2221 aattacttca ttattttcat tgcgcatcac accaattggc gctttagata aatatgtttc
     2281 attaaattga ccaacagaga aatgcatttc ctgacgatta tctaaccaca aatcactgac
     2341 atgttgaagt tcatttataa attcagttga aaacggtggt tcaataattt cgaatgaaat
     2401 attaagttca tcgaatttgt ttaaagtcgc tctaaatcca cggcgttttt tacctgaagt
     2461 tgaaaattgc gttaaatcaa taattgcttc ttcacctaat ttgaaaaatt ggttgccgaa
     2521 attatgatat aaaggcatgt gttgatctgt aacttgatag aatataacat catagcctaa
     2581 atactcagcg taattataga atgcttctaa caattcatca aaggcatttt catcaccgat
     2641 tggatcgcca agaactacta acgaacttgc tttatagcga tacattaaaa atgcattttt
     2701 atcttcatta gtaaagaact gtttatcacc actataaatt aaatgactta aataattccc
     2761 accatattga tcaataatct cttcgcactc ttcaatatta gaagatatac gcactttact
     2821 aaattgataa tcgaataacc acgcaattgc accaacaata attgcgataa ttaaaatggt
     2881 aatccagaaa taatatttaa gtacagacgt atgcatttcg attgtataaa catctaaagc
     2941 ataaaatgtt ccagctataa atatatggtt gatgtagagt ataaatatgc tgaacaaaag
     3001 cattgcgact agatttctca ttcgaattgg acgttttaac ctacgtgctc tacggaaagc
     3061 aacaataagt aaagcaaaga taataactaa ccaagttatt aaaatgtatg acgcataagt
     3121 gaataacgtc gcgacaataa ttaagataat cgaaatcatt gcataaataa tggcacgtct
     3181 actttgttta taaataccta caacattaag taaaagtaat aaacaggcac ttgtatgtat
     3241 tgccaataaa agataatacg ttaaatgatt gccatcatat aaagcatcgt atacaatcgt
     3301 caaattattt acaaagaaaa tcatacttgt aaagaatact aaaattgcca atgataatga
     3361 tggtatttta gcaataatat ctttttgata cgacattaaa aacgatgtca catctttagc
     3421 cggaatgaaa tatttagatc cttcaatata tttcttagct gaagtaccaa actcaaatga
     3481 tgaaaggatt aatgcaataa tcactggtac gaaatagtac gcaaagcgat aaagtagtag
     3541 cattaataat actttttctt caggtacacc taacgtttta aagcctaata aaacaactaa
     3601 gtcgaaggca ccaaaaccac ctggaataaa gctgactaat ccagataatg cagcaataat
     3661 gaatattgca ataaatgaca taaatgagac atggacgtca actatgacac cacagaagta
     3721 taatacaact gcagcggcta accattccac acatgacact aatgtacaat acaagcctac
     3781 ataacgattg tttttatcgg gcggtctaac cattgaatat ataataaata atggtaagaa
     3841 caacgacaca gcataaagta cccatctcac ccacgtaatt ttatttaaaa ttaatgatgc
     3901 atcaaaaaca tggaatacga ttaacaatga taacaaactt aaacctgtca acattgaaat
     3961 cagtattaat gaaataaagt gaaccaattt ctttttatca tgcgtatagt ttttgtaaac
     4021 cattgctctt acaccggcac caataaagcc accaaagcct acaatcgcat ttaatgcatt
     4081 aatgatataa cttactctta aaacttttcc taatgaaata tccattttta aagctctaga
     4141 taaaatcaca tcatacattg agagaataac aagcgatgcc ccaccaccaa taaataataa
     4201 gacaagggac atacggttga ttttactaaa ttcaaccaaa gtatctttaa agttaatccc
     4261 agataactct cgatacaacg tgattactac aaaaataaat aaagctgtag caaacgtaat
     4321 ttttaagatt gaaaatattt tgtttttaac ttcctgattc attttttcac atcaattcta
     4381 attatttctg ttataaatca aaatatatca tataaaccat aaaaatagca tatcatttac
     4441 ctttgattta tataacactt ttgtcatatt gacatctgta ttaagaagta aatagaaaat
     4501 atttctatct caaacagaac aatatggtta tctatactga ttcatattta acataaagaa
     4561 aatgatgaat caatacttat gaaaaatatt attaattaac agctgattat gagtgcttct
     4621 taatatatta aaaataccac ttgtttttat tttcgatata ataatgaagt actaatcaag
     4681 tcacaaatac tcgcttcatt atgcatgtgc tttataaatg gctaaacttt cataaaaatt
     4741 tataagctaa acatgtgctg ataatcataa atttgaacac ctaacattat aagcaccata
     4801 aaaataccaa caaacgtata gtctattggt atttttaatt acttattcaa ctattttatt
     4861 actttcttta atatcatatt cacctacatc attaccatag aaacgattgt atcgacgttt
     4921 aaataattgg aataaatgag taactaatac ttttaaaata gcatatcccg gaatacctaa
     4981 aataacgcct acaataccaa gcaatttgcc tgcacacagt aaaatgaaaa tgattgtaag
     5041 tggatgaatc ttaagtgttt tacccatgat atttggtgaa atgaagtgcc cttcaacaaa
     5101 ttgtactaaa gtccatacta ctgctaattt taagagcatc cacggcgatg ttatagcagc
     5161 tatgacaata gctggtgaaa tcgctatagt aggccctaaa tatggcacaa cacttgtaac
     5221 tgcagcaata ctagctaata ctaagctata tttcaacccg ataaccgaat aaccgataaa
     5281 caacagtata ccgatacaga atgaaacgat aatttgtcct tgaatgtatg aaccaacttg
     5341 aacactcatt ttttcaagta gatcatgaaa atcttttcgg aatttcggtg gcataatatt
     5401 cgttgaaaat tctttgaagt gatgtccatc tttaagcata aagaatagta caaatggtgt
     5461 tgtgacaatc acaacgccaa tattagcaat cgtttctgca aacgttgcaa ttttagaccc
     5521 aaagccatct gtaaattcac ttaacataga tggtattttc ttaggtaaag aatttaacca
     5581 atcattaatt tgactataat acgaagagaa aaacgtatta tttgctattt tatcaataga
     5641 attaattaat ttttctagat attgcggtga atttttaact aaactatcta cttgcgaacc
     5701 aataataggt atcaataaat taacaattaa cgtaataaca cctactacag ctagataaat
     5761 aatagaaata cctgcaactc ttggtattct ataacgctcc atcatattta cgattgggtt
     5821 aaatagatag aatagaatta aagagacgat gataggtgct gctatcgtct taaaaacgat
     5881 gataaaagga tcaaaaacgt atgatacttt ttcgaaaata aaaataacaa tacccaacaa
     5941 tactagtgct attaatgaga agactaaatc attcccacca acaaacttca tataccgtga
     6001 ttcggtaaat tttaaaaagt tctttcttat attcttttca ttttcattca attatagatt
     6061 caccaccact taaaatattc ataagatgtt gtataaaaac agtccactta tgtaaaatta
     6121 ctaacatcaa tgataccata caaataccct ttacagtaac gaaacgcccg tgtaatacac
     6181 ttaatttagg attgattaat cctttcttca aaatgatgaa tgtgtaatgt taaatatata
     6241 atttcagact cactgatatt aacatcaaat tgtttttgta tcatagttaa aattttatat
     6301 gctgtgttat agcaaatcgg atagtgattt ttaatcatag acacaaaatc atcttgtgca
     6361 tgtatatatt cttttcttct taatcggcga attaaaaatt gtacatgtct tataaaacgt
     6421 tggtattgta atgattcctt atcaacagtt gtcacaaggt ctgattcaat gatatctata
     6481 ccttttttaa taacattatt gatcaaggtc atctcatgca tagataaatc ttctgtgtta
     6541 gatgcaatat gtaatgcaat aaatcctatc tcatcttcag gaaaatgcac atctaatgct
     6601 gcatttaact gatcaattac ctgtttagca atatgatatg catcactata taactgcata
     6661 gtttccataa caaatggatt actaataact tgattttgtt ttaagcgttt ataagcaaat
     6721 ataatatgat ccgttaatga aactacaagt tgttttgaat caacattcat cgcagtatta
     6781 gaaataaaat tcaacgaatc aataattact tgtaatacat tatcatcagc gatttcaact
     6841 aaacttttat aatgtgcttt ttgttgttca ctctctaatt tataaatttt ctctattgta
     6901 atagtttggt cgtttaacgt cattccctct tttttgttaa aaccaatacc tttaccgatt
     6961 aaaataactt cttgatcatt attagtacat actacgacat tgttgttcaa tgttttagta
     7021 acaatatatt ctcccattat catcacctat ttttctatta ttaagattat atatcggaaa
     7081 tgtctaactt aaaagtataa taatttaaat acttgtgttc tattacacgc ctatcctata
     7141 tgatatatct taatttaatt tcgatgcctc tcaaagtgga ataactttaa taaaaaaatc
     7201 tgactcccat atttgcaaat aatgctattt atttatatat tatctgaatt aatactcaat
     7261 acaaactaaa aagtactatt aaattgtgca aagctaaaat aaaattatat tcatctatcc
     7321 aacaatatgt cttatcaatg gtatagtctt tgcacaccaa tgaaggaaat aaatctcaat
     7381 cttactatat taatatttaa tcaaatctta gattaactag tgtaatgata cagataatta
     7441 ttgagtacaa atttaaaacc ctgagatttt cgcattaatt tgaaaacctc agggtttatt
     7501 tgatttttat aaaatgaatc gttacattaa aaatatttat ttatcagagt tcttatattt
     7561 gttagcgccc caagcactaa ttccaaataa gttaatttct aagttttcag gtttaaaaac
     7621 aggattcttg ccttcttttt tctgcttttg ataatctttc atcaatgcaa aagctacatt
     7681 ggacagtcct ataatggaaa taatgtttac aattgccatt aagcccataa ataagtctgc
     7741 tgtattccat actgtttctg tttttacaac tgcaccgaca aagacaagta ctacaacaaa
     7801 acatctaaaa ataaataata tcacacggtt tgttgataaa aattcaatat tagattgacc
     7861 gtaatagtaa ttacctacca cagatgaaaa ggcgaataat gtaactgcta ttgttaagaa
     7921 aatacctcca gcagaaccta aatgttcatt aagtgctgat tgggtaactg caacaccttg
     7981 aggtgcgtta tcaccaaatt tcagtcctga atataataaa atcatgattg cagttgctgt
     8041 acaaaccaac attgtatcaa agaacacacc taatgattgg attaaacctt gcttgaccgg
     8101 gtgtggcact gcagcagttg ccgctgcatt cggcgcagaa cccataccag cttcattaga
     8161 gaataaacca cgtttaatac cttgaagaac cgcagcacct acagcgccac cagttacttg
     8221 ttcgatacca aatgcacttt taataatcgt acctatcata ggaacaattt gatctaaatt
     8281 aaatagcaat attactaaaa ccataccaat gtaaataata gccataatcg gtacaattaa
     8341 cgaagataac gtagcaatac tacgtacacc accaaatata ataatagcag ttacgattgc
     8401 taaaataata cctgtgatta ctggactaat attatattgc gtatttaacg actccgcaat
     8461 tgtattagat tgcactgtgt taaatacaaa tgcaaatgta attgtaatta aaatcgcaaa
     8521 tacgatacct agccattttt gatttaaacc tttagtaata tagtaagctg gaccaccacg
     8581 gaatccacca tctttatcat gtactttata aacctgagcc aaagtcgctt ctataaatgc
     8641 actcgctgca cctataaatg caataaccca catccaaaat actgcacctg gaccacctaa
     8701 aacaatcgca gtcgcaacac cagcaatatt accagtacca actctcgaac cagcactaat
     8761 cgcaaatgct tggaatggcg aaataccctt cttaccatct tctaaagttt ctggacgttc
     8821 tactaaagct ctaaacattt caggtaacat tcgtaattgg acgaatttag aactaattgt
     8881 aaagaagaat ccagctgtca ataatagacc aattaaatat tgagaccata ttaaatcggt
     8941 accaacatgg acaaattctt taaaccatcc aggaattaaa ctatcgaaat ctttcaaaat
     9001 aaacccctcg catcctctac atgaatcatg caccttctat aaaattagac cgaattgaaa
     9061 cttcagtaaa tatagagata catcatcatt tcttatacaa tacaagagat ttatattagt
     9121 ttggtcaaag tatatcgcta ttttaacgat aagtacttgg tcagcattta atataaatcc
     9181 cttgaattta gtcaaaattt aacattactg tattttatca tttaatttcg ttattgcata
     9241 tagtttttag ctaatataca tgtctattac ttcaccaaaa tcatcggtat ctacaatgaa
     9301 tgagccattt gtatattgtt cagatttatg aatatcatta attaaaccat gttcttcgtt
     9361 tgattttgaa tataatgtat attgactatg ttcacctgtc actacatgtg cagctactat
     9421 acgatgtgga tttttcttta attcttttaa taaagttatt ccacgttgtg ctcttttagc
     9481 aacttgtaag attttaaaac taatacgttt taacgagccg cgttgtgtgg ccatcaatat
     9541 agtatcattt tcagaaacac cttctgtcat aacaacgaaa tcttctgctt taagatttat
     9601 tgatttaaca ccagctgccc ttaatccggt atctgatagt tcacttgtat tataagttaa
     9661 agacatacct ttatttgtaa tgatggtaat taattgatct ttttcaaagc gcataacact
     9721 aatcaaatca tcattttctt taactttagt agcaattaaa ggtttattaa aacgcgttgt
     9781 tttaaataga ggcactgtac ttttcttaat catgccattt tgagtcgcaa aaacataaaa
     9841 tgcatctgta ttaaagtcct tttcattaaa gacattaata accacttcat cttcttcgat
     9901 aggaactatt tgtgatacat gttgccccaa ttctttccaa cgaatatctg ctaatttatg
     9961 aaccggtata aatagataac gacctttatt tgtaaatact agtacggtat cttgcgtatt
    10021 tacttcttga tgtttaagta aactgtcacc atcttttaaa ccaatatctt caacaccgct
    10081 agcattatag ctacgaatag aagtacgttt aatatatcca tgacgtgtca tacttaaaat
    10141 aacttcttca ctaggcacca taacttcttt gtcaatttta atttcttcaa tttctgcttc
    10201 aattaaagac agtcgttcag atttgaattt ctttttaatt tcattcaatt cttctttgat
    10261 gacattcaat aatgcatcat ggttatcaag aatatgacgt aattgtttga ttaatgcttc
    10321 aagttcttta tgttcacctt caagcgcaac tatgtcagtg tttgttaaac gatataactg
    10381 taacattaca attgcttcag cctgttcttc tgtgaactcg tatacttcga taaggttttc
    10441 tttagcgtca cgcttgtttt tagagctacg aatcaattcg attactttat ctaaaattga
    10501 caacgcttta atcaaacctt caacgatatg catgcgtttt tcagcattat ctaattcaaa
    10561 cttcgttcta tttgcaacaa cctcaatttg atgatttaaa taactatcta taatttgacg
    10621 aatacccatc aattttggac gaccatcact aatagcgacc atgttgaaat tatatgaaat
    10681 ctgtaaatca gagtttttat aaagataatt tttgattgat tcactgttca catctttttt
    10741 caattcaatt gctattcgta aaccagttct atcagtttca tcacgtactt caacgatacc
    10801 atcgactttt ttgtcagcac gtaattcatc gatacgtttt actaagctac ttttgttcac
    10861 ttcatatgga atttcagtaa taattaactg tttacgtcca ttgcgtaaag tttcttcttc
    10921 aactttagaa cgaactataa ttctaccttt acctgattca taagcttttt taataccatc
    10981 aataccttga ataataccgc cagttggaaa atcaggacct ttaatatatt tcattaattg
    11041 attgactgta atatccggat tatcaatata tttaagtgtt gcttgaatca cttcagctaa
    11101 attatgtggt ggtatatctg tcgcgtaacc tgcagatata cctgtagaac cattcactag
    11161 taagttagga aatcttgatg gcaataccat tggttcgagc gtcgtatcat catagtttga
    11221 aatgaaggaa actgtctctt tattaatatc acgtaataac tcttcagcta gtaagcttaa
    11281 cttagcttca gtgtaacgca ttgccgctgg tggatcatta tcgatactac cattattacc
    11341 atgcatttct attaagacat gtcgtaactt ccagtcttga cttaaacgga ccattgcttc
    11401 gtacactgag gagtctccat gtggatgata ttgaccaata acatcaccga ctgttttcgc
    11461 acttttacgg aaatttttat cgtgtgtatt accacttgaa tacattgcat ataaaatacg
    11521 acgttgtact ggttttaaac catcacgaac atctggcaat gcacgctctt gaataatata
    11581 tttactatat cttccaaagc gatcacctaa aacatcttca agtgataaat cttgaattat
    11641 ttcactcact agatttcctc ctcatcaaat tgatcatttt caagcacttg tacttcagaa
    11701 ttatctaaaa tactttggtc ctcttgcata ccaaactcaa catgcttttc aatccattca
    11761 cgtctaggtt gtactttgtc acccattaat gttgttacac gtttagatga acgcacttca
    11821 tcttcaactt gtacacgaat taaagttcgt gtttctgggt tcatcgtcgt ttcccataat
    11881 tgttcagggt tcatctcacc caaacctttg taacgttgta acgtgaagcc cttaccaagt
    11941 tctttttgta atttattaag ctcttcgtct gtccaagcgt attcaactcg ctttgttttg
    12001 cctttacctt tttccaattt ataaagtgga ggtaaagcaa taaatacacg acctgcttga
    12061 acaagcggtt tcatatattt gaagaagaat gttaacaata gcacttgaat atgcgcacca
    12121 tcagtatcag catcagtcat aataattaca cgattatagt tactatcttc aattttaaag
    12181 tcagtaccaa cgcctgcccc gattgtgtgg ataattgtat taatttcttc atttttaaaa
    12241 atatcttcca gacgtgcttt ctctgtatta attaccttac cacgtaatgg taatatcgct
    12301 tggaatttgc ggtctcgtcc aagttttgct gaacctcccg cagaatcacc ttcgactaaa
    12361 tacaattcat ttttatctgt atttttactt tgtgcaggtg ttaatttacc agatagcaaa
    12421 gtgtctttac gcttgttttt cttacctgaa cgagcatctt cacgagcttt acgtgcagct
    12481 tcccttgctt gttgtgcttt aattgctttt tttacaagtg atttagacaa ttgtcctttt
    12541 tcttctaaat agaatggtaa tttgtctgca actactgaat caacagcact tcttgcttca
    12601 gaagtaccca atttagattt cgtttgtcct tcaaattgta gtaattcttc tggaatacgt
    12661 acagacacaa cagctgttaa accttcacga atatcattac cgtctaagtt tttatctttt
    12721 gttttaagtt cattaatacg acgtgcataa tcattaaata cacgtgtcat tgctgtttta
    12781 aaaccaactt catgtgtacc accatcttta gtacgtacat tatttacaaa acttaaaata
    12841 ctttctgaat attgatcatt atattggaaa gctacgtcta cctctatacc atttgcttca
    12901 cctgaaaatg tagccacgtc atgcaaaact tcttttcctt cattgacata actaacaaat
    12961 tctttgattc cttcttcata atggtaatgc tcttgacgct ctttaccact gcgtaaatca
    13021 ttaagcgtta tttttaaatt tttcaataag aacgcagact ctt
//
LOCUS       DAMZPP010000081        12766 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMZPP010000081 DAMZPP010000000
VERSION     DAMZPP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12766
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.081"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..2511
                     /locus_tag="POH73_001739"
     CDS             <1..2511
                     /locus_tag="POH73_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HDI6887684.1"
                     /translation="EFNSFFISDIEKARKSPNQTLIDYIEGVEESKRIEVDENKEMFD
                     KFLHPSRLPDGRWPSQTEFRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIF
                     AHLVVERGKELAKLNNPKDAFVKTKTHETDDKYVYLLKESIAKYKMVVASSNNGAVEN
                     ISKDLPKIEEIIRNPEKCKFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFG
                     KSTNINQVLSHMLKQDANDIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEM
                     LKEESIRAYDVYKNCESYSKVEHELNSKKMNVKEKLNHLEIQISCDNKEIEDLDDRIN
                     YNTKQLETLNELIKSIRDSNKGFVNKLKAIFNSEEDERYKKHNAEKQQLLGQQIELEK
                     CKKIKNEDLVSKLKEKEKLIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWS
                     DNNYDERQVTNLWTSDELQYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKSRRKLI
                     DANPDKVHNAWNVMHLIFPVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVG
                     ALFRSKKVVAVGDPIQIEPVVTLESHLIDNIRKNYNVPEYLVSKEASVQSVADNANQY
                     GFWKSDATDSNQKTWIGIPLWVHRRCLKPMFTIANQIAYNNKMVLPSNITNVGKTGWY
                     DVKGNSVQKQFVKEHGEKVLELLANDWFEAIKEGKNEPSSFVISPFSAVQKQVKRILK
                     QQLPTRIDIERTKINQWVDKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPN
                     LLNVAVTRAKKEFYVIGDMQRIQSKPFYETIFKERNVK"
     gene            2594..3076
                     /locus_tag="POH73_001740"
     CDS             2594..3076
                     /locus_tag="POH73_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887685.1"
                     /translation="MKKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIESLIGLRFLKEYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKK
                     "
     gene            3281..4267
                     /locus_tag="POH73_001741"
     CDS             3281..4267
                     /locus_tag="POH73_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HDI6887686.1"
                     /translation="MKKCIKTLFLSIILVVMGGWYHSAHALDSLSKSPENWMSKLDES
                     KHLTEINMPGSHDSGSFTLTDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLQVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYPWPGYDDIVSEIIDSNK"
     gene            4488..5258
                     /locus_tag="POH73_001742"
     CDS             4488..5258
                     /locus_tag="POH73_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6887687.1"
                     /translation="MMKRLNKLVLGINLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVRSSMSIQPNGKDMNVKGMVLYMNRNSRTT
                     NGYYYVDVIERQDKGIHRDNEKKYPVKMVDNKIIPTKDIKDENIKKEIENFKFFAQYG
                     SFKDLSKYKEGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKDIEFTFVEKKGENIYFSDSLHLEPSEDK"
     gene            5352..7589
                     /locus_tag="POH73_001743"
     CDS             5352..7589
                     /locus_tag="POH73_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6887688.1"
                     /translation="MQRDYLIRVETESMLDFKRLNGLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFAESLGVGFQHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHNYHDREL
                     ILLLNDYIEEMNHFIEDIEKVNYKEITFQPTNHQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHSDFQVNEVLAYDVMPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLNQYDSRDYIDAFKVFIHHVALHVSHRKDLKFNLYVTTLHNSLIEMIDYFKALFPN
                     GGLYVHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHHLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGIYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDVTDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            7740..8918
                     /locus_tag="POH73_001744"
     CDS             7740..8918
                     /locus_tag="POH73_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDI6887689.1"
                     /translation="MNQQLIEALKSKEDKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKAMIENGVLDGVDHV
                     LGVHVMSTMETGNVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEALYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEADLEFGVEICEPQPPSED
                     FAYYAKERPSAFIYTGAAVEDGEIYPHHHPKFKISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            8920..10308
                     /locus_tag="POH73_001745"
     CDS             8920..10308
                     /locus_tag="POH73_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6887690.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGADM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRIIQGVSAACIMPSTLAIMKTYYEGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEVTNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVIIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(10794..12455)
                     /locus_tag="POH73_001746"
     CDS             complement(10794..12455)
                     /locus_tag="POH73_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDI6887691.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDVWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
BASE COUNT         4547 a         1850 c         2365 g         4004 t
ORIGIN      
        1 gaatttaata gcttcttcat aagtgatatt gagaaagcaa gaaaatctcc caatcaaact
       61 ttaattgatt acattgaggg tgtagaagaa agtaagcgca tagaagtgga tgaaaataaa
      121 gaaatgttcg acaaattttt acatccttca cgtttgcctg atggacgctg gccatcacag
      181 actgagttta gattgtcatt aatgcaacaa cttgctgtaa accaaattac gagtggtaat
      241 gaaaggataa gttcagttaa tgggccacca gggacaggta agactacttt attaaaagat
      301 atatttgcgc atctagtagt tgaaagaggt aaagagttag ccaaactaaa taatcctaaa
      361 gatgcatttg tcaaaacaaa aactcatgaa acagatgata aatatgtcta tttacttaag
      421 gaatctattg ctaaatataa gatggtagtc gcatctagta ataatggagc tgttgaaaat
      481 atatctaaag atttaccgaa aattgaagaa attataagaa atcccgaaaa atgtaaattc
      541 cctaaatatg aacagaatta tgcaaattta gcacatgaat taaaagattt tgctgaaata
      601 gctgaagatt tgattggtga aagtgcctgg ggcttatttt ctggagtatt tggtaaaagt
      661 actaatatta accaagtatt gagtcatatg ttaaaacaag atgcgaatga tattggcttt
      721 gctaaattac tacaaaatga gaataatcgt atgagttata acgagttaat gagtgaatgg
      781 caatcacatc aacgtgcatt tttagaagag ttgaggcatg ttgaaatgtt aaaagaagaa
      841 tctattagag cctatgatgt ttataaaaat tgtgagtctt actctaaggt tgaacatgaa
      901 ctaaatagca aaaaaatgaa tgttaaggaa aagttgaatc atttagaaat tcaaatatct
      961 tgtgacaata aagaaattga agatttggat gatcgaatta attataatac aaagcaactc
     1021 gaaactttaa atgagttaat taaatccatc agagatagta acaaagggtt tgttaacaag
     1081 ctgaaggcga tatttaattc agaagaagat gaaagatata aaaaacataa tgcagagaag
     1141 caacaattat taggacaaca gatagagtta gagaaatgta aaaaaattaa aaatgaagac
     1201 cttgttagca aactaaaaga aaaagagaaa ttaattaaac aattaactaa agtacagttg
     1261 caattagacg agttaaattc acagttacaa gagttagaag catatcgtat tgagtcaaaa
     1321 attacaattc cagaaaaaga tttttggagt gacaacaatt atgatgagcg ccaagttact
     1381 aatttgtgga cgagtgacga acttcaatac agacgtgcca tgctcttttt aagagcaatg
     1441 atattgcata aattattatt gattgctaat aatacaacta tttattatgc gattaatgat
     1501 tttaaaagta gaaggaaatt aattgatgca aatccagata aagtacacaa cgcatggaat
     1561 gtgatgcatt taatatttcc agtagttagt acgacgtttg caagctttaa atctatgtat
     1621 gggggcatac caaaagattt catagactac ttatttattg atgaagcagg acaagcaata
     1681 cctcaagcag ctgtgggagc attatttaga tcgaaaaaag ttgtagccgt aggtgatccg
     1741 attcaaattg aacctgttgt tactttagaa agccatttaa ttgataatat tcgtaaaaat
     1801 tataatgttc cggaatatct agtttctaaa gaagcttctg tgcagtctgt cgctgacaac
     1861 gctaatcaat atggcttttg gaaatctgat gctactgata gtaatcaaaa aacgtggata
     1921 ggcatacctt tatgggtgca cagacgatgt ttaaaaccca tgttcacgat agctaatcaa
     1981 attgcttata ataataaaat ggtgctacct agtaatatta caaacgtagg taagaccggt
     2041 tggtatgacg ttaaaggaaa ctcagtacaa aaacaatttg tgaaagagca tggcgaaaaa
     2101 gtattggaat tactagctaa tgattggttt gaagcgatta aggaaggtaa aaatgagccg
     2161 agttcatttg taatatcacc attttcagca gtacaaaaac aggttaaacg tatattaaag
     2221 caacaactac cgactagaat tgatattgaa cgtacaaaaa ttaatcaatg ggtcgataaa
     2281 tccattggta ctgttcatac ttttcaaggt aaagaggctc aaaaggtgta ttttgtaata
     2341 ggtactgata atactcaaga tggtgctgtg aactggtcat gcgaaaaacc aaacttgtta
     2401 aatgttgcag tgacaagggc taaaaaagag ttttatgtaa ttggtgacat gcaaagaata
     2461 cagtcgaaac cattttatga gaccattttt aaagaaagaa atgtaaaatg aaatgacagc
     2521 atttctatag gctaatcaag tgatacttaa ttaccgcgca atttatacat tttaaaagga
     2581 gtaaaattga ataatgaaga aatttaacaa ctggatatta aatgcaataa gtggatctca
     2641 aacagacaag aatgaaacaa ctgaagaatt aaaaggggca aaatttatta ttttatatgc
     2701 atattcaatg ctcgttttgc ttgcgttagt aatttctaac atattcattc acattttgga
     2761 gcctaaacta tcaatcacca ctcaaatcat catcgttttg attttaattg aatcactaat
     2821 tggactgcgt ttcttgaaag agtacgatgt taagcgtggc aaagataaag aaaataagaa
     2881 aaatagtaag gatttcgtta aactaaaatc aattttagta gcaattttat ttacatcatt
     2941 ggcgctgaca gcaggcactg tagctgatat atacggtttc actgacttag gaaatactag
     3001 aagtgattta atcgtttgga gcataggtgg tattatattt ggcctcgtat gttacacaat
     3061 ggaagataaa aaataacgat aaggagctgg cgattataaa gctagctcct tttttaactt
     3121 atatatgtaa agaactattc taaggatttt taatcatatg tcaataattc ctataataca
     3181 ttattaaaac atcaattaaa taagttttaa aattttacac atatttttat ttaaaaaaga
     3241 tgtataatta atgtattaaa tatagaaaga agttgatatt atgaaaaagt gtattaagac
     3301 tttgttttta agtatcattt tagtagtgat gggtggttgg tatcattcag cacatgcgtt
     3361 agattcgttg agtaaaagtc cagaaaattg gatgagtaaa cttgatgaaa gcaaacattt
     3421 aactgagatt aatatgccag gctcacatga tagtggctca ttcactttaa ctgacccggt
     3481 aaaatcagtt tgggcaaaga ctcaagataa agattacctt actcaaatga agtcaggagt
     3541 caggtttttt gatattagag gtagagcaag tgctgataat atgatttcag ttcatcacgg
     3601 catggtttat ctgcatcatg aattaggaaa atttctcgat gatgctaaat attatttgag
     3661 tgcctatcca aacgaaacaa ttgtgatgtc tatgaaaaag gactacgata gcgattctaa
     3721 agttacgaag acatttgaag agatttttag agaatattat tataataacc cgcaatatca
     3781 gaatcttttt tacacaggaa gtaacgcgaa ccctacttta aaagagacaa aaggtaaaat
     3841 tgtcctattc aatagaatgg gtggtacata cataaaaagt ggttatggtg ctgacacgtc
     3901 tggcattcaa tgggcagata acgcgacatt tgaaacgaaa attaataatg gtagcttaaa
     3961 tttacaagta caagatgagt ataaagatta ctatgataaa aaagttgaag ctgttaaaaa
     4021 tttattggct aaagcaaaaa cggatagtaa caaagacaat gtatatgtga atttcttaag
     4081 tgtagcgtct ggaggcagcg catttaatag tacttataac tatgcatcac atataaatcc
     4141 tgaaattgca aaaacgatta aagcaaatgg gaaagctaga acgggttggc tgattgttga
     4201 ctatgcaggg tatccgtggc ctggatatga tgatatcgta agcgaaatta tagatagtaa
     4261 taaataagga ttcaataatg atattaagac gagtatgaaa atagttagat tctaattatt
     4321 ttcactactc gtttttattt tgaaaataag taatgattca acaatattat aaattgaaca
     4381 gattgtttgt ggaatttttg ataatattaa agtgaaaaag tgttataaat tgatattaat
     4441 atgtaatttg caaaagtaaa tcattttaaa aagaagagag ttgtaagatg atgaaacgat
     4501 taaacaaatt agtgctaggt attaatcttc tgtttttagt cattagtatt actgctggtt
     4561 gcggcatggg taaagaagcg gaaattaaga aaagttttga gaagacattg agtatgtatc
     4621 cgattaaaaa tctagaggat ttatacgata aagaaggtta tcgtgatgat caatttgata
     4681 aaaatgataa aggtacatgg attgttcgtt cgtcaatgtc gattcaacct aatggaaaag
     4741 atatgaacgt aaaaggtatg gttttatata tgaatagaaa ttcaagaaca acaaatggct
     4801 attactatgt tgatgttata gaaagacaag ataaaggtat acatcgtgat aacgaaaaaa
     4861 agtatcctgt taaaatggtc gataataaaa tcattccaac aaaagatatc aaagatgaga
     4921 acataaaaaa agaaatcgag aactttaagt tcttcgcgca atatggcagc tttaaagatt
     4981 tgtcgaagta caaagaggga gatatttcat ataatccaga ggtgccgagt tattcagcca
     5041 aatatcaatt aacgaatgat gattataatg taaagcaatt acgtaaaaga tatgatatac
     5101 caacaaataa agcaccaaag ttattgttga aaggtacagg taatttaaaa ggctcatcag
     5161 ttggatataa agatattgaa tttacgtttg tagagaaaaa aggagaaaat atttacttta
     5221 gtgatagcct acatcttgaa ccaagtgagg ataaataatc atccacacac acgattcaat
     5281 atgaaattca aatatgttgc tgtaaaataa tgtaaaataa acgtattcat attactgaat
     5341 tgagggatag tatgcaacgt gattatttaa ttcgagtaga aactgagagt atgttagatt
     5401 tcaaaaggct caatggttta atgattggtt ttgttattaa aggtgaggca cacatttatg
     5461 atgaaaataa tatgacgcaa tgcaacagtg gcgacatttt catcattaac caccgcgact
     5521 tgtatcgatt tcaacttcaa caagatggca tcatatgtta tatccaattc caaatgaaat
     5581 atttagcaga caagtttgat gatgtgcatt gtctatattt tcacttaaca gatgcgacca
     5641 caacaaaaaa tatacatcaa ctgagaaata taatggcaag actggtttca acacatattc
     5701 gacataatga gttgtctaaa ttgactgagc aacaacttgt cattcagttg cttatgcata
     5761 tgattcatta tgttccgcgt acatatcatt cgaaccaaag tatcttaaat gatgataaag
     5821 tgaatcaagt atgcgactat atcgagttac attttcatga agacttaagc ctttcggaat
     5881 taagcgaata tgttgggtgg tcagagagcc atctgtctaa aaagtttgca gaatcgctag
     5941 gtgtaggatt ccagcatttc ttaaatacga cgcgaattga gcatgcgaaa ctcgatttga
     6001 catacacaga tgaaacgatt actgatattg cattgcaaaa tggcttttca agtgcagcga
     6061 gctttgcgag aacatttaaa cactttacgc atcaaacgcc taaacaatat cgaggtgatc
     6121 gtccagcaat cactgaaaat cagcaatcgg cacaacataa ttatcacgac cgtgaattga
     6181 tattactttt aaatgactac attgaagaaa tgaatcattt cattgaagat attgaaaagg
     6241 tgaactataa agagattacc tttcaaccaa ccaatcacca actaaatcaa tttaatcata
     6301 ttattcaagt gggctatttg aggaatttgc tcaatacaca gtatcaatca cagttgctta
     6361 catgtcatag tgattttcaa gtcaatgaag tattagcata tgatgtgatg ccatatatta
     6421 tgaaaaagct caatgcgcca ttcacgtatg atgcagaaat ttcgaatata ttttatgata
     6481 ttgatttgtg tttagacttt ttattagatc ataattttag tctaaccatg catttgaatc
     6541 agtatgactc acgagattat atcgatgcat tcaaagtatt tatccatcac gttgccctgc
     6601 atgtcagtca tagaaaagat ttgaagttca acttgtatgt gacgacattg cacaattctt
     6661 tgattgaaat gattgattat tttaaagcat tattccccaa tggtggcttg tacgttcact
     6721 tagatcaagc tacggaaaga catctaccat tgttgaaacg acttgagcca cacatcgacc
     6781 attttgtatt tgatgctaat tcaaatgatg ctgttgattt taataaaatg aatgatgatg
     6841 aatttaaaac cgcgagtcaa atgattatta ataaaacgaa ttaccttatc gacttaatgc
     6901 atcgtcatca cctaaagcgt ccactcattt tactcaattg gaatacattg acgggtgata
     6961 catttattac aaacggcgaa tattttagag gtggtatcat cattgagcaa ttattaaaat
     7021 taagctcaaa ggtagagggg atcgggtatt ggttgaatta tgatttgcac gtcagtcatt
     7081 gtaaaaatga acgggattat atgaattcta ttgaactgtt tcatcaatat aatggaaaac
     7141 gtccggtcta tttcacggca ttgttattta ataaattaac aagcaatatt ttatattctg
     7201 atgatacatg tattgtcacg ggaactgatt caaattttca aatattgtta tatgatgcaa
     7261 agcattttaa tccatactta gcgttggaca atcaaatgaa tatgcgtgcg acggaaatga
     7321 ttcatttgaa cataaatgcc ctagaagaag gtatatataa gattaaacat tttaccttag
     7381 ataaagaaaa tggtgcatta tttaatcttt ggcgaaaaca tcatacgata catggcatgg
     7441 acaaggactc tatagattac gttaatcgaa tgagttttcc aaaattagaa gtgtacgata
     7501 tagatgtgac ggatacgctg gcattaaaca ttaaaatgat tacgaatggg attcacttaa
     7561 ttgaagtaaa acgttaccca agttcataaa atgatcacaa atcgcaaatt ttgttataca
     7621 taatttgtga ttttttgtat tcaaagttaa aattgcaaaa aattaatggt taacatctct
     7681 gttgttggca atataaataa tgatcaatca tatatgatgt aactaaggag atgaatgata
     7741 tgaatcaaca attaattgaa gctttaaaat caaaagaaga caaaatgatt gagatcagac
     7801 gttatttaca tcagcatcca gaattatctt ttcatgagga tgaaacggcg aaatacatcg
     7861 ctgaatttta caaaggtaaa gatgtagaag tagaaacgaa tgtcggacca cgtggaatta
     7921 aagtaacgat tgattcaggg aaacctggta aaacattagc aatccgtgca gactttgatg
     7981 cattacccat tactgaagat acaggattat cttttgcatc acaaaataaa ggtgttatgc
     8041 acgcatgtgg tcacgatgca catacagcat acatgcttgt attagcagag acgcttgccg
     8101 aaatgaaaga tagttttaca ggaaaagtcg ttgtgataca tcaaccagct gaagaagtac
     8161 caccaggtgg tgctaaagca atgattgaaa atggtgtatt agacggtgtt gatcatgtat
     8221 taggtgtaca cgtcatgagc acaatggaaa caggtaatgt gtattacaga cctggttatg
     8281 ttcaaacagg acgcgcattc ttcaaattga aagttcaagg taaaggtggt catggttcat
     8341 caccacatat ggccaatgat gccattgttg caggtagcta cttcgtcaca gcgttacaaa
     8401 cagttgtatc tagacgacta agtccatttg aaaccggtgt tgtcacaatc ggttcatttg
     8461 acggtaaagg tcaattcaat gttattaaag atgttgttga aattgaaggt gatgtacgtg
     8521 gattaacaga tgctacaaaa gcaacaattg aaaaagaaat taaacgttta tcaaaaggat
     8581 tagaagcatt gtatggtgta acttgcacat tagaatataa cgatgattat cctgcattat
     8641 ataatgatcc agagtttact gagtacgtgg ctaagacgct aaaagaagca gaccttgaat
     8701 ttggtgtcga aatatgtgaa ccacaaccac cttcagaaga ctttgcatac tatgctaagg
     8761 aacgaccaag tgcctttatt tatacaggtg cagctgtgga agatggtgaa atttacccac
     8821 atcatcatcc taaatttaag atttcagaaa aatcattact tatttcggca gaagctgtag
     8881 ggacagttgt tttagattac cttaaaggag ataactaaca tgaatgaaac gtatcgcggg
     8941 ggcaacaagt taatcttagg tattgtatta ggtgttatta cattttggtt gtttgcacaa
     9001 tcacttgtaa atgttgtgcc aaatttacaa caaagttttg gtgcagacat gggaacaatt
     9061 agtattgcgg taagtctaac cgcactattt tcaggcatgt ttgttgttgg agcaggcggc
     9121 ctagcagata aaattggacg cgtgaaaatg acaaatatcg gcttattatt aagtattatt
     9181 ggttcagcat taattattat tacaaattta ccagcattgc taattttagg ccgtattata
     9241 caaggcgtat cagcagcgtg tattatgcct tccacattgg ccattatgaa aacttattat
     9301 gagggtgctg aacgtcagcg tgccttaagc tattggtcta tcggttcttg gggtggaagt
     9361 ggtatctgtt cactcttcgg tggggcagtt gcgacaacta tgggttggag atggattttc
     9421 atcttctcaa tcatcgttgc cgtactttca atgttactca tcaaagggac gcctgaaacg
     9481 aaatcagaag ttaccaatac acataaattt gacgttgcag gtctaattgt tctagtagtt
     9541 atgttattaa gtttaaacgt tgtcattact aaaggtgcag cacttggtta tacatcatta
     9601 tggttctttg gtttgattgc aatcgtaatt gtagcattct ttattttctt aaatgttgag
     9661 aaaaaagtag ataatccgct tattgatttt aaattatttg aaaataaacc atatacaggt
     9721 gcaacgattt cgaacttctt attaaacggt gttgcaggta cattaattgt agcgaataca
     9781 ttcgtacaac aaggtttagg ttatacagca ttgcaggcag gatacttatc aatcacttat
     9841 ttaatcatgg tgttattgat gattagagtt ggtgaaaaat tattacaaaa aatgggttct
     9901 aagcgaccaa tgttattagg tacattcatt gtgatcattg gtattgcact tatttcatta
     9961 gtattcttac caggcatatt ttatgttatc agttgtgtcg taggatattt atgttttgga
    10021 ctaggcttag gtatttatgc aacaccttct acagatacag ctatttcaaa tgcaccgtta
    10081 gataaagttg gcgttgcttc aggtatttat aaaatggctt catcacttgg tggtgcattc
    10141 ggtgtcgcaa ttagtggtgc agtatatgcc ggtgcagttg ctgcaacgag cattcataca
    10201 ggtgcgatga ttgcactttg ggttaacgta ttaatgggaa tcatggcatt tatcgcaatt
    10261 ttatttgcga ttcctaatga tgataaacgt gtcaaagatg cgaaataata acgatgacat
    10321 gcccagtgtt ttgataatga tttaaatgaa aataaagcaa attaaatgag gaaatgtata
    10381 gagcagtgca tatagataaa tctatgattg gattaacact aatataaata taaataaaaa
    10441 accatccact taaacttttg ataagttagg tggatggtta tttattgcgt tgttctaaaa
    10501 atttggacat ttatatggaa agaactatat ataatgtgaa ggttatggca attcgcttca
    10561 atgaaggcga cgtgtaaaag tgtatattaa acgtttcggg gtcagactcc gagaacagtg
    10621 tccgtcatgc ttaccgcata ctaatgaagc attattatat cagtggttct agccgattta
    10681 agataataaa ttttgctaaa caaaaaagcc aacccatgaa tgttggattg gctttttaca
    10741 tgcatctgaa tctctaattt taaaaaatat gaatataaat aagacattaa aaattaaatt
    10801 tcagttgttg caatttcttc atctgtaggt acatcatcgt taagaccaac aagtgcttca
    10861 gaaacatttc gtgaatgata accgatacgt tcaagaacac caatcatatc gatatatagt
    10921 aatccgcctt ttgttgtaca ttcaccacga ttaaggcgtt taatatgacc tttgcgtagt
    10981 ttatgttcaa tattaaatga ttctctacta cgctctacaa tttcatcttt tttcgttttg
    11041 tcataaacat ctaacatgtc aatggcttta tcaaatgact cagcaacatg gttgaataat
    11101 ttatccatac cgcgttgtgc atcttctgta atgcgaatat cttcatcatg ttggcgtttt
    11161 aattgagcga catactcttc tgttagctct gctactttta aaatagagcg attgacatca
    11221 aacataactg ctaaacgctc aacgtctgcc ttcgtaatgg cttttgtaga aattctaact
    11281 aaataatttc gaatactatc attgattgtt tcaacagctt gatgcttttg ttcaagcttt
    11341 ttgattaatt ttttgtcgtc ttttgtaatt tcgcgaatgt cttcaaacat tgataagaca
    11401 atctgaccca cattttgtaa ttctttttga gtttcttgta atgcaacacc aggtgcgtga
    11461 taaacaagat ctttgtttaa gtgctgaggt ttatagtcat cagcaatatc tttacctggg
    11521 acaagctttg taactatcca tgctaaacct gctacaaatg gtaattgaat caaagtattt
    11581 gttatgttga agataccatg tgatactgca atcgtcatcg ctggttttaa gtgccataca
    11641 tcttgtaaca aactaatcaa atgaatcaca actggcaaga aaattgtgaa gataattacc
    11701 ccgattaagt taaagatgac gtgtacaagc gccgcacgtt ttgcagcgat tgagccggct
    11761 aaactagcta agatagctgt aatcgttgta ccaatattat cgcctagtaa cacagggatt
    11821 gctgcgttta agctaattaa atcttgttga taaaattctt gtaaaatacc aatcgtcgca
    11881 cttgaacttt gaactagtgc tgttaaccct gcgccgacaa tgacagcaag tattggattt
    11941 gtagacatat caagcattaa ttgcttaaat ccatctaatg atgctaaagg tttaacggca
    12001 tcacccataa attctagacc gaagaataga gaaccgaaac cgaatagtat gcggccaatg
    12061 ttattgattt tagagcgctt aaagaaaaag attaaaaatg cacctaatgc taaaattggc
    12121 attgcatatt cgcctaaatc tataccgata ataaatgctg ttaccgttgt tccgatatta
    12181 gcacccatta tcactccaat ggcttgtttt aacgtcataa atccagctgt taccagtccg
    12241 attgtgataa ctgtcgtacc tgaactactt tgtattaaaa tagttacaac gatacctgca
    12301 ataacaccta atactggatt tgatgtaaat ttgtttaaaa tatctcgtag cctgtctcct
    12361 gctgatgctt gaagtccgtc tcccatgatt tttaagccgt aaaggaaaat acctaaacca
    12421 cctaaaaagg agaaaatgac ttctgtaacc gacatttcca ttatttcacc tcaaataagc
    12481 tttatatttt gattatcgct tataattgta aatttaatgt taagattagg taaaattatt
    12541 taacaatata tgttatttgt atatgacttg taaaatatcg tcacttatta tgtaaatttt
    12601 cagtgtgaaa tggcaggttt gtaatcactt gtttaacaaa atgatgcaat caatcatgca
    12661 attatgtgtc atcaaaaaaa tcatgtgagt gggataatga aataaagttt gtgaacatat
    12721 gatttctatc ccactccatg atttgaaatc accaaataaa aatcta
//
LOCUS       DAMZPP010000082        12545 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMZPP010000082 DAMZPP010000000
VERSION     DAMZPP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12545
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.082"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..702
                     /gene="pde2"
                     /locus_tag="POH73_001747"
     CDS             <1..702
                     /gene="pde2"
                     /locus_tag="POH73_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HDI6887692.1"
                     /translation="VCDTANAPRIDDERYSTGRKLIKIDHHPAVDQYGDINLVNTNAS
                     STSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTGRFLFNNTSEHTMEIAGKLIGHD
                     IDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFCQVKITEDVLEQFGIQPNEASQF
                     VNTIADIKGLKIWVFAVDEGSEIRCRLRSKGQLIINDIAQDFGGGGHPNASGVSVNSW
                     DEFEQLAKALRTKLN"
     gene            723..3920
                     /locus_tag="POH73_001748"
     CDS             723..3920
                     /locus_tag="POH73_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDI6887693.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDTCIANNIKPIFGMTIYVTNGLNNIETVVLAKDNYGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKNVADEHRDIVRVFDSHEDTYLDHRSVLVQGIKHVWIQD
                     VCYQTRHDADTISALAAIRDNTKLDLIHDQEDFGAHFLTENEIHQLDVNPEYFTQADR
                     IAQKCNAELKYHQSLLPQYQTPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRREKVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGTKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVEERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNNKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDLR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPSDIRLI"
     gene            4369..5598
                     /locus_tag="POH73_001749"
     CDS             4369..5598
                     /locus_tag="POH73_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDI6887694.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTMAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            5793..6650
                     /locus_tag="POH73_001750"
     CDS             5793..6650
                     /locus_tag="POH73_001750"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDI6887695.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            6650..7594
                     /locus_tag="POH73_001751"
     CDS             6650..7594
                     /locus_tag="POH73_001751"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDI6887696.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVEQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            7863..8831
                     /gene="pfkA"
                     /locus_tag="POH73_001752"
     CDS             7863..8831
                     /gene="pfkA"
                     /locus_tag="POH73_001752"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDI6887697.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQFINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            8853..10610
                     /gene="pyk"
                     /locus_tag="POH73_001753"
     CDS             8853..10610
                     /gene="pyk"
                     /locus_tag="POH73_001753"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDI6887698.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIEEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVTNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNV
                     LVTIDAAQGKIFEGYANVL"
     gene            complement(10957..12318)
                     /locus_tag="POH73_001754"
     CDS             complement(10957..12318)
                     /locus_tag="POH73_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6887699.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAYKTQFGHASFTNLYEHGVFPKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKTPDLHKKA
                     TYKLFGGKYMGYLIFVFFIFVFGLLFINVETRRAIYFIPIWFILLGFMYLRYKRIAAK
                     SNN"
BASE COUNT         4468 a         1753 c         2393 g         3931 t
ORIGIN      
        1 gtatgtgata ctgccaatgc tccacgaatt gacgatgaac gttatagtac aggtcgtaaa
       61 cttattaaaa tagatcatca tcctgcagtt gatcagtatg gtgatattaa tctagttaat
      121 acgaacgcgt catctacaag tgaaatcatt tacgatttaa tctcacattt taatgatgaa
      181 gcaattgtta ataaagatat cgcgagtgtt ttataccttg gtatcgtcgg tgatactggg
      241 cgattccttt ttaacaatac ctcagaacat actatggaaa ttgctggaaa gttaattggg
      301 catgatattg atcataatgc gcttttaaat aaaatgatgg aaaaggatcc aaaaatgttg
      361 ccgtttcaag gttatgtttt acaacatttc gaacttatgg atgacggatt ttgccaagtt
      421 aaaataactg aagatgtatt ggagcaattc ggtattcagc caaatgaagc atctcagttt
      481 gttaatacaa ttgctgacat caaaggtttg aaaatatggg tgtttgcagt tgatgagggt
      541 agtgaaataa gatgtcgctt acgctctaaa gggcaattga ttattaatga tattgcgcaa
      601 gattttggtg gcggtggtca tccgaatgcg tcaggagttt cagtgaacag ctgggatgaa
      661 tttgagcaac ttgctaaagc tttacgcacg aaacttaact aatagaaagg ggccattcaa
      721 tcatggtggc atatttaaat attcatacgg cttatgattt gttaaattca agcttaaaaa
      781 tagaagatgc cgtaagactt gctgtgtctg aaaatgttga tgcacttgcc ataactgaca
      841 ccaatgtatt gtatggtttt cctaaatttt atgatacatg tattgccaat aatataaagc
      901 cgatttttgg tatgacaata tacgtgacaa atggattaaa caatattgaa accgttgttt
      961 tagcaaaaga taattatggt ctgaaggatt tatatcaact atcatcggaa ataaaaatga
     1021 atgcattaga acatgtgtct tttgaattat taaaacggtt ttctaacaat atgattatca
     1081 tttttaaaaa tgttgcagac gaacatcgtg acattgttcg agtttttgat tcgcatgaag
     1141 atacgtattt agatcatcga agtgttttag ttcagggtat aaagcacgta tggattcaag
     1201 atgtatgtta tcaaacacga catgatgcag atacaatttc tgcactcgca gcgataagag
     1261 ataatacaaa attagactta atacatgatc aagaagattt tggtgcgcat tttttaactg
     1321 aaaatgaaat tcaccaactt gatgtgaatc cagaatattt cacacaagct gacaggatag
     1381 ctcaaaagtg taatgcagaa ttaaaatatc atcaatctct acttcctcaa tatcagacac
     1441 ctaatgatga atcagctaaa aaatatttgt ggcgtgtctt agttacacaa ttgaaaaaat
     1501 tagaacttaa ttatgacgtc tatttagagc gattgaaata tgagtataaa gttattacta
     1561 atatgggttt tgaagattat ttcttaatag taagtgattt aatccattat gcgaaaacga
     1621 atgatgtgat ggtaggacca ggtcgtggtt catctgcagg gtctctggtc agttatttat
     1681 tgggaattac aacgattgat ccgattaaat tcaatctatt atttgaacgt tttttaaacc
     1741 cagaacgtgt aacaatgcct gatatagata ttgactttga agatacacgc cgagaaaagg
     1801 tcattcagta cgtccaagaa aaatatggcg agctacatgt atctggaatt gtgactttcg
     1861 gtcatctgct tgcaagagcg gttgctagag atgttggacg aattatgggg tttgatgaag
     1921 ttacattaaa tgaaatttca agtttaatcc cacataaatt aggaattaca cttgatgaag
     1981 catatcaaat tgacgatttt aaaaagtttg tacatcgaaa ccatcgacat gaacgctggt
     2041 tcagtatttg taaaaagtta gaaggtttac caagacatac atctacacat gcggcaggaa
     2101 ttattattaa tgaccatcca ttatatgaat atgccccttt aacgaaaggg gatacaggat
     2161 tattaacgca atggacaatg actgaagccg aacgtattgg tttattaaaa atagattttc
     2221 tagggttacg aaatttatca attatccatc aaattttgac tcaagtcaaa aaagatttag
     2281 gtattaatat tgatattgaa aaaattccat ttgatgatca aaaagtgttt gaattgttgt
     2341 cgcaaggaga tacgactggc atatttcaat tagagtctga cggtgtaaga agtgtattaa
     2401 aaaaattaaa gccggaacac tttgaggata ttgttgctgt aacttctttg tatagaccag
     2461 gtccaatgga agaaattcca acttacatta caagaagaca tgatccaagc aaagttcaat
     2521 atttacatcc ccatttagaa cctatattaa aaaatactta cggtgttatt atttatcaag
     2581 aacaaattat gcaaatagcg agcacttttg caaacttcag ttatggtgaa gcggatattt
     2641 taagaagagc aatgagtaaa aaaaatagag ctgttcttga aagtgagcgt caacatttta
     2701 tagaaggtac aaagcaaaat ggttatcacg aagacattag taagcaaata tttgatttga
     2761 ttctgaaatt tgctgattat ggttttccta gagcacatgc tgtcagctat tctaaaattg
     2821 catacattat gagcttttta aaagtccatt atccaaatta tttttacgca aatattttaa
     2881 gtaatgttat tggaagtgag aagaaaactg ctcaaatgat agaagaagca aaaaaacaag
     2941 gtatcactat attgccaccg aacattaacg aaagtcattg gttttataaa ccttcccaag
     3001 aaggcattta tttatcaatt ggtacaatta aaggtgtagg ttatcaaagt gtgaaagtga
     3061 ttgttgaaga acgttatcag aacggcaaat ttaaagattt ctttgatttt gctagacgta
     3121 taccgaagag agtcaaaacg agaaagttac ttgaagcact gattttagtg ggagcgtttg
     3181 atgcttttgg taaaacacgt tcaacgttgt tgcaagctat tgatcaagtg ttggatggtg
     3241 atttaaacat tgaacaagat ggttttttat ttgatatttt aacgccaaaa cagatgtatg
     3301 aagataaaga agaattgcct gatgcactta ttagtcagta tgaaaaagaa tatttaggat
     3361 tttatgtttc gcaacaccca gtagataaga agtttgttgc caaacaatat ttaacgatat
     3421 ttaaattgag taacgcgcag aataataagc ctatattagt acagtttgat aaagttaaac
     3481 aaatccgaac taaaaatggt caaaatatgg cattcgtcac attaaatgat ggcatcgaaa
     3541 ctttagatgg tgtgattttc cctaatcagt ttaaaaagta cgaagagttg ttatcacata
     3601 atgacttatt tatagttagc gggaaatttg accttagaaa gcaacaacgt caattaatta
     3661 taaatgagat tcagacatta gccacttttg aagaacaaaa attagcattt gccaaacaaa
     3721 ttataattag aaataaatca caaatagata tgtttgaaga gatgattaaa gctacgaaag
     3781 agaatgctaa tgatgttgtg ttatcctttt atgatgaaac gattaaacaa atgactactt
     3841 taggctatat taatcaaaaa gatagtatgt ttaataattt tatacaatcc tttaacccta
     3901 gtgatattag gcttatataa cttttatcaa ctaataaatt atgatatagt aaactgatgg
     3961 ttagatattt tttaaccatc agtttacgtt tatattagtt ttttatgctt attattttta
     4021 tgagtttcac tttacattat tgattaatcc ataagaataa ttagcacaaa aagcagtata
     4081 cataaattga gtaaagaatt ttgtcgatat atcgtgagaa aaaataataa ctttttgata
     4141 tattggtttt gctatccatt ataataatta aaggtatatc tttagatagt atggtttaat
     4201 gtatctttat aaatattatt ttattatcgt ttatcgctat ttgtgttaca taattaaaac
     4261 tataagttct tactcattca gatataccca atatttttac ttttataatg taatgcgttt
     4321 tatccaagtt attttttaaa aataaatatt gaattagggg ctgatttcat gtcattaaga
     4381 gatgaagcat tggaaatgca caaacgtaat caaggtaaat tagaagttaa accaaatgta
     4441 aaagttacta ataaagaaga attaagttta gcatactcac ctggcgttgc tgaaccgtgt
     4501 aaagatatat atgaagacaa aagaaaagta tatgattaca caattaaagg aaatacagtt
     4561 gcagttatta ctgatggaac agcggtatta ggtttaggta acattggacc tgaagcaagt
     4621 attcctgtaa tggaaggtaa agcagtatta ttcaaaagct tcgctggtat caatggggtg
     4681 cctattgcgt taaatacaac tgataccgaa gaaatcatta aaacagttaa attgttagaa
     4741 cctaattatg gtggtattaa tttagaggat atttcggcac cacgttgttt tgaaattgaa
     4801 gaacgattga aaaaagaaac taatattccg gtattccatg acgatcaaca tggtacagca
     4861 attgtaacaa tggcaggttt ggtaaatgca ttgagagttg ttaacaaaga tattgctaaa
     4921 ataaaagttg tactaaatgg tgctggtgca gcaggaatag ccattgttaa attactatac
     4981 gcgtatggtg taagaaatat ggttatgtgt gactcaagag gcgcaatttt tgaaggacgt
     5041 tcatatggta tgaatcctac gaaagatgtt gtagctaagt ggacaaataa agacaagatt
     5101 gaagggtctc tagaagaagt cgtaaaagac gcagatgtat ttattggagt ttctgtagct
     5161 aatgcgctgt cacaagatat ggttaagagt atggcagata atccaattat atttgcaatg
     5221 gctaatccaa atcctgaaat aatacctgat gatgccaaag cagcaggtgc acgcgttgtt
     5281 ggtacaggac gttcagacta tcctaaccaa attaataatg tattagcttt ccccggtatt
     5341 tttagaggtg cattagaggt tgaagctaca catataaatg aagaaatgaa aaaagcagct
     5401 gtagaagcga ttgctgattt aattgatagt tctgaattaa atgaagacta ctgtatccca
     5461 ggaccgtttg ataaacgtgt agcgccatca gttgctcgta atgttgctaa agcggcaatg
     5521 gaatctggag tagctaggat tgaagttgat ccgcaagatg tgtatgataa aacaatgaaa
     5581 cttacagatt tacaataaaa ataattaacg tttaattaaa gtgcttaaaa gttagcaata
     5641 aagagtcatt aaaatgatga aaatgaaagt ttatgataaa cattcaacag tcaaacgaat
     5701 ataaatcaaa taaatttaaa cccgttttta actggtcaag ttcagtttaa ggctctaaat
     5761 ggttagaaca gaggttattt ggaggttttc ctatgtttaa agattttttt aatcgaacaa
     5821 agaaaaagaa atatcttaca gtacaagact ctaaaaataa tgatgtgcct gcaggtatta
     5881 tgactaagtg tccaaagtgt aagaaaatta tgtacacaaa agaattagct gaaaatttaa
     5941 atgtgtgctt taattgtgat catcatattg ctttaactgc gtataaacgt atagaagcaa
     6001 tttctgatga aggatcattt acagaattcg ataagggaat gacctctgcg aatccattag
     6061 attttccaag ttatttagaa aaaattgaaa aggaccaaca aaagacaggt cttaaagaag
     6121 cagtagtgac tggtacagcg caactagatg gtatgaaatt tggcgttgct gtcatggatt
     6181 cacgttttag aatgggaagt atgggatcgg ttattggtga aaagatatgt cgcatcattg
     6241 attactgcac tgagaaccgt ttaccattta tacttttctc tgcaagtggt ggtgcacgta
     6301 tgcaagaagg tattatttcc ttgatgcaaa tgggtaaaac cagtgtatct ttaaaacgtc
     6361 attctgacgc tggactatta tatatatcat atttaacaca tccaactact ggtggtgtat
     6421 ctgcaagttt tgcatcagtt ggtgatataa atctaagtga gccaaaagca ttgataggtt
     6481 ttgcaggtcg tcgagttatt gaacagacaa taaacgaaaa attgccagat gatttccaaa
     6541 ctgcagaatt tttattagag catggacaat tggataaagt tgtacatcgt aatgatatgc
     6601 gtcaaacatt gtctgaaatt ctaaaaatcc atcaagaggt gactaaataa tgttagattt
     6661 tgaaaaacca ctttttgaaa ttcgaaataa aattgaatct ttaaaagaat ctcaagataa
     6721 aaatgatgtg gatttacaag aagaaattga catgcttgaa gcgtcattgg aacgagaaac
     6781 taaaaaaata tatacaaatc taaaaccatg ggatcgtgtg caaattgcgc gtttgcaaga
     6841 aagacctacg acactagatt atattccata tatctttgat tcgtttatgg aactacatgg
     6901 tgatcgtaat tttagagatg atccagcaat gattggtggt attggctttt taaatggtcg
     6961 tgctgttacg gttattggac aacaacgtgg aaaagataca aaagataata tttatcgaaa
     7021 ttttggtatg gctcatccag aaggttatcg aaaagcacta cgtttaatga aacaagctga
     7081 aaaattcaat cgtcctatct ttacatttat agatacaaaa ggtgcatatc ctggtaaagc
     7141 tgctgaagaa cgtggacaaa gtgaatctat tgcaacaaat ttgattgaga tggcttcact
     7201 aaaagtacca gttattgcga ttgtcattgg tgaaggtggc agtggaggtg ccctaggtat
     7261 tggtattgcc aataaagtat tgatgttaga gaatagtacc tactctgtta tatctcctga
     7321 aggtgcagcg gcattattat ggaaagacag taatttggct aaaattgcag ctgaaacaat
     7381 gaaaattact gcccatgata ttaagcaatt aggtattata gatgatgtca tttctgaacc
     7441 acttggcggt gcacataaag atattgaaca acaagcttta gctattaaat cagcgtttgt
     7501 tgaacaatta gattcacttg agtcattatc acgtgatgaa attgctaatg atcggtttga
     7561 aaaattcaga aatatcggtt cttatataga ataatcaact taagcatttt tatgttaaat
     7621 cgttactggg ttttaccata aattgaagta cattaaaaca ataattcaat atttagatac
     7681 tgaattttaa ctaagattag tagtcaaaat tgtggctact aatctttttt taattaagtt
     7741 aaaataaaat tcaatattta aaacgtttac atcaattcaa tacattagtt ttgatggaat
     7801 gacatatcaa tttgtggtaa tttagagtta aagataaatc agttatagaa aggtatgtcg
     7861 tcatgaagaa aattgcagtt ttaactagtg gtggagattc acctggaatg aatgctgccg
     7921 taagagcagt tgttcgtaca gcaatttaca atgaaattga agtttatggt gtgtatcatg
     7981 gttaccaagg attgttaaat gatgatattc ataaacttga attaggatca gttggggata
     8041 cgattcagcg tggaggtaca ttcttgtatt cagcaagatg tccagagttt aaggagcaag
     8101 aagtacgtaa agttgcaatc gaaaacttac gtaaaagagg gattgagggc cttgtagtta
     8161 ttggtggtga cggtagttat cgcggtgcac aacgcatcag tgaggaatgc aaagaaattc
     8221 aaactatcgg tattcctggt acgattgaca atgatatcaa tggtactgat tttacaattg
     8281 gatttgacac agcattaaat acgattattg gcttagtcga caaaattaga gatactgcgt
     8341 caagtcacgc acgaacattt atcattgaag caatgggccg tgattgtgga gatctagcat
     8401 tatgggctgg attatcagtt ggtgctgaga caattgtagt tccagaagtg aaaacagata
     8461 ttaaagaaat agctgataaa attgaacaag gtattaaacg tggtaagaaa cactcaatcg
     8521 ttcttgtagc agaaggttgt atgactgcgc aagattgtca aaaagaatta tcacaattca
     8581 tcaatgttga taatagagtg tctgtgttag gtcacgttca acgtggtggt agcccaacag
     8641 gtgcggatag agttttagca tcacgattag gtggatatgc ggtagactta ttaatgcaag
     8701 gtgaaacagc taaaggtgtt ggaattaaga acaataaaat tgtagcaaca tcttttgatg
     8761 aaatttttga tggtaaagat cataaatttg attatagtct atatgaactt gctaacaagt
     8821 tatctatata agatttcagg aggaattata aaatgagaaa aactaaaatt gtatgtacaa
     8881 ttggaccagc ttcagaatca gaagaaatga ttgagaaatt aatcaatgct ggtatgaacg
     8941 ttgcacgatt aaacttttca catggtagtc atgaagagca taaaggtaga attgatacaa
     9001 ttcgtaaagt agctaaaaga ttagacaaaa ttgtagcaat tttattagat acgaaaggtc
     9061 cagaaattcg tacgcataat atgaaagacg gtatcattga acttgaacgt ggtaacgaag
     9121 ttattgttag catgaatgaa gttgaaggaa cacctgaaaa gttctctgta acttatgaaa
     9181 acttaattaa cgatgttcaa gtaggttcat acattttact tgatgatggc ttaattgaat
     9241 tacaagttaa agatattgac catgctaaaa aagaagttaa atgtgatatt ttaaactctg
     9301 gtgagcttaa aaacaaaaaa ggtgttaact tacctggtgt aagagttagt ttacctggta
     9361 ttacagaaaa agatgctgaa gatatccgtt tcggtattaa agaaaatgtt gacttcattg
     9421 cagcaagttt cgtacgtcgt cctagtgatg ttttagaaat tcgtgaaatt ttagaagaac
     9481 aaaaagctaa catttcagta ttccctaaaa ttgaaaacca agaaggtatt gataatattg
     9541 aggaaattct tgaagtgtct gatggtttaa tggttgcacg tggtgacatg ggtgttgaaa
     9601 tcccacctga aaaagtacca atggttcaaa aagatttaat cagacaatgt aacaaattag
     9661 gtaaaccagt tattacagct acacaaatgt tagattctat gcaacgtaac ccacgtgcta
     9721 cacgtgcaga agctagtgac gttgccaacg caatctatga tggtacagat gcagtaatgt
     9781 tatctggtga aactgctgct ggtttatatc ctgaagaagc tgttaaaaca atgagaaata
     9841 ttgctgtatc agctgaagca gcccaagatt acaaaaagtt attgtcagat cgtactaaat
     9901 tagttgaaac ttcattagtg aatgctatcg gtatttcggt tgcacataca gctttaaact
     9961 taaatgttaa agcaattgta gctgctactg aaagtggttc aacggcacgt actatctcta
    10021 aatatcgtcc gcattcagac attattgcag tgactccaag tgaagaaact gcacgtcaat
    10081 gttcaattgt ttggggtgtt caacctgtag ttaaaaaagg acgtaagagt acagatgcat
    10141 tgttaaacaa tgcagttgca acagctgttg aaactggtag agtaactaat ggtgatttaa
    10201 tcattattac tgctggtgta ccaactggtg aaactggaac tactaatatg atgaaaatcc
    10261 acctagttgg tgacgaaatt gctaatggtc aaggtattgg acgtggatca gttgttggta
    10321 ctacgttagt tgctgaaact gttaaagatt tagaaggtaa agatttatct gacaaagtta
    10381 tcgttacaaa ctcaatcgat gaaacgtttg taccttatgt agaaaaagcg ttaggcttaa
    10441 ttacagaaga aaatggaatt acatcaccaa gtgcaattgt tggtttagaa aaaggtattc
    10501 caacagttgt aggtgtagaa aaagctgtta aaaacataag caataacgtg ttagttacga
    10561 ttgatgctgc tcaaggtaaa atctttgaag gatatgcaaa cgtactataa tttataaaaa
    10621 acgtctttcc atttatcaac aatggaaagg cgttttttgg ttcatctggt attttatgac
    10681 gtaattaata ggatatttga taatgataat gtatgaatag caatctatat aaatgtttat
    10741 atcttttata catgcacatt atcagcttta aactttcact catattactt tggaaagtta
    10801 ttataaaata gaagtatgga tgtatcttta aaatgataca ttattcaata gatgagaaag
    10861 taaaagtatt gaggcaagta cgcaatttaa tattataagt tgcatataaa aacccctgtt
    10921 gaactttaat atattcaaca ggggtaaaac ttgttgttag ttatttgatt tagcagcgat
    10981 acgtttatat cttaagtaca taaatcctaa tagtataaac caaatcggaa taaagtatat
    11041 cgcacgtctt gtttcaacat tgataaacaa taggccaaac acaaatataa agaatacaaa
    11101 aatgagatag cccatatatt ttccgccaaa taatttatac gtagcctttt tatgaagatc
    11161 tggagtttta cgactataat tgatataagc gatgataatc agtccccaaa caactaagaa
    11221 taatactgta gagattgttg tcacatacgt aaatacctta gtagcatcag ggaaaatata
    11281 gtttaataat gctgccacga gtaacaatgc tgatgaagca aagatcgcta catgtggaac
    11341 gccatactta ttcgtcttag agaaattcgg tggtgcttgt tgttgacttg ataaaccgaa
    11401 cagcatacga ctattcgaga aaataccact gttacatgat gaagcagcag cagttagtac
    11461 tacaaaatta atcaagcctg cagcaaatgg aattccaatt aatgcgaata attttacgaa
    11521 tggactgtta tcaggatcaa cttgttgcca aggaataatc gacatgataa ccgctagagc
    11581 accgacataa aatattaaaa ttctaatagg aacactatta atcgcttttg gtattgtttt
    11641 aacaggatct ttcgtttcac ctgccgtaac accaatcatt tcgattccca cgaatgagaa
    11701 taaggccatt tggaatgaca taaagaatcc agatgctcct ttagggaata caccgtgctc
    11761 gtataggttc gtgaaactcg catgtccaaa ttgagtttta tatgcaaata gaatcataac
    11821 gaaacctacg acaattaaac caatgattgt tgctatttta atgatagaga accaaaattc
    11881 taactctcca aatagtctcg cactcaataa gttaaaagac attaataaca atacgcaaaa
    11941 aagtgcacta atccagtttg gaatttctgg gaaccagaag cttacatact ttgctacagc
    12001 tgttacttca gccatacctg taataatcca acagaaccag tatgtccaac cggttacgaa
    12061 tcctgcaaaa ggcccaatgt atgtattggt tacatctgcg aaagatttaa attcagtatt
    12121 ttgaatgatg atttcaccta aacctcgcat aaacataaat aacataaatc caataatgat
    12181 atatgttagt aatatagaag ggccagttaa tgcaatcgtt tgaccagcgc ctaagaatag
    12241 ccctgtacca attgcgccac caatagcaat taattgaatg tggcgattgc tcagttccct
    12301 ttgtaatttt tcagccataa tacatctccc ttaaatatag atatgtttat tatgcactta
    12361 tattaggata tatacaatta ttttcggtaa aaatgtgtaa aattccatgt taatatactt
    12421 tggattttat aatcatatat aataaccaat tgaaaatttt attctatgct aaaagttggt
    12481 gatgaatata atcttgaaaa agctttaatg gtgctatttg tggccattct gtgacattta
    12541 catag
//
LOCUS       DAMZPP010000083        12303 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMZPP010000083 DAMZPP010000000
VERSION     DAMZPP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12303
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.083"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(124..3576)
                     /locus_tag="POH73_001755"
     CDS             complement(124..3576)
                     /locus_tag="POH73_001755"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDI6887700.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKAAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(4129..5355)
                     /gene="ftsW"
                     /locus_tag="POH73_001756"
     CDS             complement(4129..5355)
                     /gene="ftsW"
                     /locus_tag="POH73_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDI6887701.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            complement(5669..5944)
                     /locus_tag="POH73_001757"
     CDS             complement(5669..5944)
                     /locus_tag="POH73_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDI6887702.1"
                     /translation="MAKQTTMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            6085..6567
                     /locus_tag="POH73_001758"
     CDS             6085..6567
                     /locus_tag="POH73_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887703.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            6569..6736
                     /locus_tag="POH73_001759"
     CDS             6569..6736
                     /locus_tag="POH73_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HDI6887704.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(6875..8722)
                     /gene="typA"
                     /locus_tag="POH73_001760"
     CDS             complement(6875..8722)
                     /gene="typA"
                     /locus_tag="POH73_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDI6887705.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTLINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            8824..9015
                     /locus_tag="POH73_001761"
     CDS             8824..9015
                     /locus_tag="POH73_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HDI6887706.1"
                     /translation="MKQKKSKNIFWVFSILAVIFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(9169..9996)
                     /locus_tag="POH73_001762"
     CDS             complement(9169..9996)
                     /locus_tag="POH73_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDI6887707.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWVVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPVLGEIFKDVVNISRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILNE
                     VDGLASNLLGEPLTISGPNSILVGNRGVHREILGVYLEPHRDALIQLHEQRFKRKSK"
     gene            10153..10767
                     /locus_tag="POH73_001763"
     CDS             10153..10767
                     /locus_tag="POH73_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HDI6887708.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLHELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATSKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLKEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            10951..12303
                     /locus_tag="POH73_001764"
     CDS             10951..12303
                     /locus_tag="POH73_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDI6887709.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQG
                     "
BASE COUNT         3993 a         2257 c         1795 g         4258 t
ORIGIN      
        1 ttttgcgaaa atatcatttc tgtcccactc cctttttata tcaatattta aaattaaacc
       61 taagacaagt aaaaacgaga ttacacgtca attgttgtgt aatctcgttt tattttaatc
      121 attttagtca gttgcttttt caatttcgat taataaatcg cctgtcgcta ttgtgtcacc
      181 attatttaca gttacttgtt taatcacacc gtcaaatggt gcttgaattg ttgtttccat
      241 tttcatagct tcagtaatta gcaacggctg attagctttc acagtttcac ctacactaac
      301 cttgacttca gttactgaac ctggcatttg cgcaccgata tgacttggat tactcttatc
      361 tgcttttggc ttaacgttcg catttgtatg cacattttca tctttaatat aaatacgtct
      421 cgcttgacca ttcatcgcat agtaaatcgt tctattacca ttttcatctg gttcactaat
      481 cgtttctagt ttaataatta atcgtttacc tttatcgatt tcgatttcta ctgtttcacc
      541 attacgcatt ccaaagaaga atgtaggcgt atcaagtaac gataagtttc cgtattgatt
      601 tctagtttga atatattgtt catatacttt tggatataat acataactaa taatatcttg
      661 ctccgtaaca ggaccttgtt gctcttcttc aagcaactca cggacttttt caaaatcaac
      721 tggctctaga tattcacctg gacgagctgt taatgcttct tggcctttta aaataaccgc
      781 ttgtaaatct ttattaaaac catttacagg ttgtcctatt tcacctttga agaacgacac
      841 tactgattct gggaaatcta atttatagcc atctgtaata acggattgtt catcaagatc
      901 attttgtacc atataaagtg ccatatcacc aactacttta gacgatggcg ttacttttac
      961 gatatcacca aataagaaat tcactctgcg atacatatct ttgacttcat caaatctttc
     1021 gcctaaacct aaacttttag cttgttgact taaattcgaa tactgtccac caggcatttc
     1081 atgttgataa atttcagtat tcggtgattt gatatcactt tcaaagtctg aataataagt
     1141 acgtacagtt gaccaataat gactaagtga ctccatacct tcaatatcag ttctaaggtg
     1201 acgtgggaag ccatttaatg cataatataa cgaattggcg cttggctgac ttgttaaacc
     1261 actcattgaa gcaacagcag tatcaatgat atcgacacca gcatctattg cttgtttgta
     1321 cgttaataaa ccattaccac ttgtatcatg agtgtgaaga tggattggta aatctacagc
     1381 tgcttttaac tcaccaatca attcgtaagc agctttaggt tttaataagc ctgccatatc
     1441 tttaatcgct aaaatatgga aaccttcacg ttctaactct ttagctagtt tgacataata
     1501 ctctaaagta taaatgtttg atcgctcagg atttaaaatg tcacctgtat aacaaatagt
     1561 accttctgag attttgcccg cttcttgtac tgcttcattg gcaactttca tttgatctac
     1621 ccagtttaat gaatcgaaaa ttctaaagac atctatgcct gctttagcac tttcttgtac
     1681 gaatttatga ataacattat caggatagtt tttataacca actgcgtttg aagcacgtaa
     1741 caacatttgg aataatacat ttggaatagc tttacgtaga cgttcaagtc gttcccatgg
     1801 gttttccttc aagaaattat atgccacatc aaatgtagca ccgccccaca tttctagtga
     1861 gaaaccatct ttaaatacgt ccgctgtttt ggatgcgata ttaatcatat ccttagttct
     1921 aactcgtgta gctaataatg attggtgtgc atctctaaag gttgtatctg ttagtaagac
     1981 atcatcctgc tttttaaccc attcagctac accttttgga cctacttcat caagcaattg
     2041 tttcgtacca ctaaatgaag cgattttact tgaagataca gttggaattg atgctaattc
     2101 atagtctggt ttcggacgtt tctcaacatt tgggaaacca ttaattgtta cattacctat
     2161 atattctaat gttttagtac ctctatctag agacggctga atgtcgaaaa gttctggtgt
     2221 ttcttcaata aatttagttg tgtaatcacc acttgtgaac tttttattct tcattacatt
     2281 aattaagaat ggaatattag ttttaacacc acgaatacgc atttctcgta atgaacgtac
     2341 cattttttct tctgcttgtt taaatgatat cgcatgtgta gataatttta cgagtaatga
     2401 atcataataa ggtgatatct cagcaccttg gaaaccatct ccagcatcaa gacgtacacc
     2461 aaagccaccg cttgaacgat aagcaatgat tgttccagta tccggcataa aatcatttaa
     2521 cggatcttct gttgtaatac gacattggat ggcatagcct aatgttgtaa tatctttttg
     2581 ttgcggcata ttaatctctt caccaaataa atcggcacct gctgcaacta aaatttgtgt
     2641 cttaacaata tcaattcctg ttaccatctc tgtaattgta tgctctactt gtacacgagg
     2701 gttaacttct ataaagaaga attcgtcacc agatactaga aattcaacag tacctgcatt
     2761 gacatattta atattttcca tcaattgaat tgcagcatca caaatacgtt gacgtaatgt
     2821 tgatgataat ccaactgatg gtgcaacttc tacaactttt tgatgacgac gttgtactga
     2881 acaatcacgt tcaaataagt gtacgatatt tccatgttcg tcacctatga cttgtacttc
     2941 aatatgcttt ggattatcaa tgtatctttc tatgtaaact tcactattac caaatgattt
     3001 ttctgcttct gattttgctc tatggaaagc atcttctaat tcactttctt cacgaacgat
     3061 tctcatacct ttaccgccgc caccacttgt ggctttaatc attagcggga aaccagcttc
     3121 ttctgcaaat tcttttgcta attcatatga tttaattgga ccgtctgtac caggaataac
     3181 tggtaaatct gccttgatag ccgttgtacg agctttaact ttatctccaa acatatctaa
     3241 atgttctaaa tgaggaccaa taaatttaat cccttcttct gcacaacgac gcgcaaattg
     3301 ttcattttca cttaaaaatc catagccagg atgaatcgca tccacattcg cttgttttgc
     3361 tacatcaatg atacgctcaa tatttaaata actttcagca ggacctaaat cacttccaac
     3421 taaataggat tcatctgctt tatatctatg taatgaactt ttgtcttcat tcgaataaat
     3481 tgcaactgtg ctgatgtcta attctgctgc cgctctgaat atacgaattg caatttctcc
     3541 acggttagca acaagtaact tttttatttg tttcaatagc gatacactcc tcaaactatt
     3601 agaattttct aactaattag ataataaaat tttatcttaa agcgctctgt tttgctatag
     3661 ttatgtttca aattttcaaa atttaacatt cttgagacaa ttaaaacctc cgcttcaaaa
     3721 ataataattt caaaaaggat tatgcaacaa caggtagttc acgtttttgt tgtgatacat
     3781 tttcgatttc tacaactcta aaaaattaaa aataaaattg taaaacatca acatttatta
     3841 tcaatagcga taactttatc ttatcatcat gattctaatt tcgccaccac atttagtgat
     3901 ttttagtcat aaaatttagt tataattata cgttgttttg tttataaaat ttgataatca
     3961 tgagtaatct cgtaatatca aaacaaaaag gaagttaagc gttgtttggt tgcttaactt
     4021 ccgttattga actcatcagt ccaatttgac aaagagtata taaatagcca atgtgtagca
     4081 ttgcttaatg acagaagtca tctcgcgcta aatattggct agtatttttt aattaaattg
     4141 tcttcttata tcaacttttt gttgttgttt ctttcgttgc tggtctactt tgatttgttt
     4201 acctacaatc agaagtaatc ccatagcaat acttaaacta atcattgatg atccaccaaa
     4261 gctgataaat ggcaatggca caccagttaa tggaattgtt gccgaaatac caccaatgtt
     4321 tacaaacgtt tggcttccaa agtatgtggc aatcccaaca cacacaagtt tataaaaata
     4381 tgatgatgtt ttatttgcaa actggaaggc acgatataca ataaagaact ctaaagtaat
     4441 aactagcaat cctccgatta aacctaattc ttcgcaaata attgcaaaaa taaaatctgt
     4501 atgtggttct ggtaaatagc ccaatttcat tgcactattt cctaatcctt ttccaaatac
     4561 gccaccgtta cctatcgcaa gcaatgaatt ggaaatatgg tatccagttc ctgattcgaa
     4621 ttggaatgga tctgttagcg tactaaatct ggcagttaaa taacttggta accaaccagc
     4681 cattaatgca atgacaaata ctactaagaa tcctagtact gctggtattc caaatcttag
     4741 gactttgttt accccaattc ctgaataaaa aataatcgca actaaaataa ttaatattag
     4801 taatgtttgc cctacgtctt tttgtaagaa aactaaaaac gtacaaccta atgctaatac
     4861 aataggactt aaaattaatt ttggtttact taatactcta ggcatttttt tactaatcat
     4921 aaatgggata tataatatga ttgcaatttt taataactca gatgcctgta agttcataaa
     4981 tcctaagttt atccaacttt tagaaccatt aatatcttta ccgattacta acgtcagtaa
     5041 taatagcgag acgatagtta taatcatacc tttttgcact ttaatattac tcagtaattt
     5101 aacattcatt aaaaatgcaa taaaaaatac aattataaaa ctcattatga catatgctaa
     5161 ttgtcggttg taaaaatacg ttcctggaac atcgatacca ccagtcaatg tacctttagt
     5221 tgctggaacc atactggcac tatataccat cactaatcca attaaactca atacaatata
     5281 tgtaactaat aacgggtaat caataaactt tgaggtttta ccaatatacc gtaaaatact
     5341 tctaaaattc ttcatatatt cagtcatcca attctacaat ttatacttca aatgatttcg
     5401 ttttatactt taattttaaa aatccatgtt acttattata tacatgacac agtcataatc
     5461 acaaatatta acaggtctct tgaggtaatt tatagaatat ttgtttactt aatatcgtga
     5521 attgctaaaa cccaataaaa gtgactgaca tgtcatcaag aagacaacat aacagccact
     5581 ttaatcaata attaaattat aaatataaaa tcggcgctgc tcaattgtcg atatctcacg
     5641 ccgttttaat atgcttatat attaaacctt aaacaagtgt aaaagcttca tgtaattttg
     5701 aaagttcttt ctcaagacgt aacataattt gtttgccatc ttcgcggtca actaaaccta
     5761 atttaacagc aaaatcaact tctttttgaa gtccaaacat ttgtgtatct agtacttctt
     5821 catataatgg gcatgaaggt aatgttaaat tatctagttg aactttaatc agatgcaaga
     5881 tttcatcagc atctttagtc aattgtttca aagctgcatt tttcattgtt gtttgtttcg
     5941 ccatgacacg ctccccctct atataaattc tcaagtctta ctaaaagttt atcttactaa
     6001 atattaaaaa gcaagtagat ttaaaatgtg tcgttagcaa tttgtgttaa aataatgcta
     6061 tatacattga tagggagtgg gaaaatgata caaattaaag gagctgtaaa gtttcctata
     6121 acattagata gtacgacttg gattttcgat gatagaaaag ttagcatcga agatttagaa
     6181 aaaggtgtat ttgatggttc aaaaccaatc gactttgaag ataatcatga atggaatcgt
     6241 gctatcctag aaggacaaac taatccgcct actttaaatt cagaaataaa atataaaaaa
     6301 cgtgccatgt tagaaggttc atttgtcatt aatatgacac catttttcaa acattccgaa
     6361 ccacatgact ctgcaactac tattcgatta tcaaatgagc atgatcattt agatataccg
     6421 atagatctat tgccctattt atttttccaa tttgcaaaag acggtaaacg actttacagc
     6481 gataatggtg tagatagttt tatttacaat caagaagatg gttattcata tcgaatcaac
     6541 catgtaactc atattgaggt gatttaattt gagacaagtg caatgcatta tctgtgatgc
     6601 aaaagtattt atagatgagc gcacaactga gtctaaacga cttaaaaaca atcctattcg
     6661 aacatttatg tgcgatgatt gtaaaagtcg actagacacc cctaaacaac gtgcgcaaca
     6721 ctatccgctc gattaatatc tgaattaaca agttagaaat ttgaaaatgt gaaagtaaca
     6781 ggaactgata gcattaaaac actttttcaa atattatatg ccaaaagaga agctaagcaa
     6841 ctttcatcgc tcagcttctc ttttattatg ttaattattc attttcttgc atcatttgtt
     6901 tgatacgctt tgcttctttt tcacgaacat ttttgtttaa aattttcttt cttaaacgta
     6961 tgctttctgg agtaacctca acaagttcat catcattaat gaattgtaac gcttcttcca
     7021 atgttagtat acgcggtcta ttcattgttt gtgtttggtc tttcgttgca gaacgtacgt
     7081 tagtttgatg ttttgtttta gtgatattaa cagttaaatc attttcacga ttatgttcac
     7141 caacaatcat accttcataa acttcagtac caggttccat gaagtttaca cctctatctt
     7201 caagtcctaa aatggcataa gtacttgcag aaccttgatc cattgaaatt aacgcaccat
     7261 tacgacgacc gccaatttgc gctttaatac gtggtctaaa ttcttcaaat gtatggttaa
     7321 taataccgta acctcttgtc attgacataa attcagtcgt ataaccaatc ataccacgag
     7381 ccggtacatt aaagattaaa cgtgtaagtc cattatcagt tgtagtcata tcaaccattt
     7441 cacctttacg tgcacctaat gattcaataa ccgcacctgc attttcttgt ggcacttcac
     7501 attgcacacg ttcaaatggt tcacacatta caccatcaat ttctttaata attacttgtg
     7561 gttttgaaac ttgtaattca taaccttcac gacgcatatt ttcaataagg attgataaat
     7621 gcaattcacc tcgaccagca actacccatg tatctggaga atctgtgtta gaaactttca
     7681 aagatacatc tgtttctaat tgttgattta aacgttcttg aatttgacgc gctgttacaa
     7741 agtcaccttc acggccagca aatggtgaat tgttaacttt aaatgtcatt tcaagagtag
     7801 gctcatcaat acgtagaact ggcaatgctt cttgatggtc atgtggtgtt acagtttcac
     7861 caacattaat gtcttccata cctgaaacag caattaaatc tccagcttgt gcttcttcaa
     7921 tttctaaacg ttttaatcca aagtaaccaa agattttagt tacacggaag tttttcactg
     7981 taccgtctaa tttaattagt gatacattat ctccgacacg cattttacct ctgaatacac
     8041 gaccaatacc aatacgacca acataatcat tgtagtccaa caatgctact tggaattgta
     8101 atggctcatc actgttatca attggagctg gtacataatc aataattgtt tcatataatg
     8161 attgtaaatt atcatcttgt ttttcaggat ctaagctagc tgtaccattt actgctgaag
     8221 cataaacaac agggaattct aattgttcat cgtttgcttc taattcaata aataaatcta
     8281 aaacttcatc tacaacaccc tctggacgtg ctgatggttt atcaatttta ttaacaacaa
     8341 caacaggttt taggttttgt tctagcgctt ttttaagtac aaaacgtgtt tgaggcattg
     8401 taccttcata cgcatctact actaagacaa ccccatcaac cattttcata atacgttcta
     8461 cttctccacc aaagtcagca tgtcctggtg tatccaaaat attaataagt gtacctttat
     8521 aatcaacagc cgtatttttg gctagaatcg taattccacg ctctctttcg atatcgttag
     8581 agtccattgc acgttcatcg acatgttcat tttctctgaa tataccagat tgttttaaca
     8641 actcatctac taaagttgtt ttaccatggt caacgtgagc aataattgct atattgcgga
     8701 catcttctct tttattagtc atataaatag tttccttctt tcttaagtaa aatcccactt
     8761 ttaattacaa cttttttatt atatcatata attgagaaaa gataagcaaa aggtggggat
     8821 aggatgaaac aaaaaaaatc taaaaatatc ttttgggtat tttctatatt agctgttatt
     8881 ttcttagtat tatttagttt tgctgttggt gcatcaaatg taccaatgat gattttaaca
     8941 tttatattac tcgttgcaac ctttggaatt ggatttacta caaagaaaaa atatcgagaa
     9001 aacgattggc tataagttat atcgacaata acacgcgttg attaaagacc acacatgttc
     9061 atgatgaaca tttaaatggt tttatattgg cgcgtgtttt tattatttaa gatataaaaa
     9121 cgtaatatgt cacctaattg ttaggactac atattacgct tttggtaatt attttgattt
     9181 tcttttaaat cgttgttcat gtaattgtat taacgcatcg cgatgtggct ctaaatatac
     9241 accgaggatc tctctatgaa caccacgatt accaactaag attgaatttg gaccactaat
     9301 tgttaatggt tctcctagta aattagaagc tagaccgtcc acctcattta aaatgactag
     9361 tcctccagca aaatcccacg gttggagtct aggtgtcata tatgcttcta aattaccggt
     9421 agcgacagaa acaatttcaa gcgctgcact accatatgcc cttgcacttc ttgaaatatt
     9481 tacgacatcc ttaaaaatct ctcctagcac aggtttagtc aaccagttcg gattgatccc
     9541 aataatgctt tgtcttagat tagaatcatt caatggtttc aagggttggc tacctcgata
     9601 tgcaccttcc cctactttag catgatataa gacatcagcc ataacatcat atacaaaacc
     9661 tgcataaggt ttaccatcga tataaatacc aattgaaatc gcgaaatttt cttgttgatg
     9721 aacaaaattc aatgtaccgt ctattgggtc aacaacccat accgtacctt tggaagtatc
     9781 gatgtcatga ccatgccctt cttcacctaa tacttgatga ttgggatatg tttctaaaat
     9841 tgtatcaaaa ataaaatctt ctgttgcttt atctacattt gtaacaaggt cattcggatt
     9901 tgactttgtt tcaattgtta aattttgctc catcaattgt ttaattctaa ttcctgcttc
     9961 ttgaataagt ccttgggcaa atccataaag tgccataaca tcatctccta acatctttat
    10021 tatacatcaa cattttataa aaataacttc ttatgataaa aatgaaaata ctaattttaa
    10081 tgagagcgtc ttaatccgca attgataaaa catatgctac aatattttta aacactatac
    10141 aggaggtact ctatgacaaa atatacattt aaacctaaag atttcaaagc attcaacgta
    10201 gaaggcttag acgcacgaat ggaagcttta aacgaataca tacgaccaca actccatgaa
    10261 ttaggagaat attttagtga cttctttaca agtcaaactg gtgaaacatt ttatcctcac
    10321 gtagcaaagc atgctagaag aagtgtgaat cctcctaaag atacatgggt tgcttttgct
    10381 acaagtaaac gaggctataa aatgttacct catttccaaa ttggtatgtt tgaagatcaa
    10441 ctgtttgtta tgtttggaat catgcatgaa gcaaaagata aagcaacacg tgcaaaagtt
    10501 tttgaaagaa aatttaaagc tattcaacaa ttacctgatg attatcgtgt atgcttagat
    10561 catatgaaac ctgataaacc atttattaaa gatttaacgg atgatgattt aaaagaagcg
    10621 atacaaagag ccatcaatgt gaaaaaaggt gaattcttta tagcgcgtgc aatcacacca
    10681 caagataaaa gattaaaaag tgacaaagca tttattgcat ttttagaaga aaccttcgat
    10741 cagttcttac cattttattc tgcataaata actttgttta aataatagag cacgtaatca
    10801 catccatgat ttcgtgctct ttttacttac cattaaattg aacgttcaac ataataattc
    10861 atacttttaa aaaaattaaa ataaatttag gttgacctaa acgttttatt aggttattat
    10921 attgtccata agaagtagag gtgagtcaaa atgaataata aacgacattc aacaaatgaa
    10981 caattaagtt tagacgaaat aaacaataca attaaattcg atcatcgcag ttcaaataaa
    11041 cagaaatttt tatcatttct tgggcctggg ttattagtcg ctgttggtta catggatccc
    11101 ggaaactgga taacatcaat gcaaggtggc gcgcaatatg gctatacttt gctattcgta
    11161 attcttattt caagtttatc agcaatgtta cttcaaagca tgacagtgag attaggaata
    11221 gcaacaggta tggacttagc tcaaatgaca cgacattatt tatcaagacc tattgctata
    11281 atcttttgga ttattgcaga actagcaatt atcgccacag atattgctga ggttattggt
    11341 agtgctattg ctctcaatct cctatttaac atacctttaa ttgtcggtgc actaattact
    11401 gtacttgatg tatttttact actatttata atgaaatatg gttttagaaa aattgaagct
    11461 attgttggta cattaatttt cacagtgtta ttcatcttta tatttgaagt ctatatttca
    11521 tcaccacagt tgaacgctgt gttaaatgga tttattccac atagtgaaat cattacaaat
    11581 aacggcattc tctatattgc attaggtatt attggagcaa caattatgcc tcataatttg
    11641 tacttacatt catcaattgt acaatctaga acatactcaa gacataacaa tgaagaaaag
    11701 gcgcaagcga ttaaatttgc tacgatagat tcgaacattc agttatcaat cgcatttgta
    11761 gtcaattgct tattattagt gttaggagca tcactatttt tcaactcaaa cgctgacgat
    11821 ttaggtggtt tctatgattt atatcacgcc ttaaaaactg aacctgtact aggtgcaaca
    11881 atgggtgcaa tcatgagtac attatttgca gttgcattat tagcgtcagg tcaaaattca
    11941 acgattactg gtactttagc aggacaaatt gtaatggaag gatttttaag attacacata
    12001 ccaaattggt taagacgttt aattacacgt tcccttgctg tcattcctgt tatcgtatgt
    12061 ttaatcattt ttaaaggtaa tgcagctaaa attgagcaac tacttgtatt ctctcaagta
    12121 tttttaagta ttgctctgcc attctgttta ataccattac aattagctac tagtaataaa
    12181 gacttgatgg gacctttcta taataaaaca tgggttaata tcatttcatg gacacttatt
    12241 attattctaa gtattttaaa tgtctattta atcgttcaaa cattccaaga actacaaggt
    12301 taa
//
LOCUS       DAMZPP010000084        12302 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMZPP010000084 DAMZPP010000000
VERSION     DAMZPP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12302
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.084"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..1065
                     /locus_tag="POH73_001765"
     CDS             <1..1065
                     /locus_tag="POH73_001765"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDI6887710.1"
                     /translation="LKILTEVELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLT
                     GRLSGNIGYVASKVAQEVKPTDYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHE
                     NLENYQNAKKQIYKNQTEEDYLICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDG
                     FIVYKGVRIINTEDLVLPGEHNLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQ
                     YVGTNRTNKYYNDSKATNTLATQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRA
                     MVVFGQTKAKFAKLGNSQGKSVIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYS
                     TFEERGEKFIERFRAHLPSY"
     gene            1081..2400
                     /locus_tag="POH73_001766"
     CDS             1081..2400
                     /locus_tag="POH73_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HDI6887711.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEQGNEENNDSASSHANDNNIDDSTDSNIENEDYR
                     YNQETDDQNESNGIAVDNEQHQSAPNEQNSDSNDEETVTKKERKSKVTQLKPLTLEEK
                     RKVRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEEDPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLARDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSTQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            2506..3912
                     /gene="ftsA"
                     /locus_tag="POH73_001767"
     CDS             2506..3912
                     /gene="ftsA"
                     /locus_tag="POH73_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDI6887712.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESSDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKEESKFKKLMKSLFE"
     gene            3945..5117
                     /gene="ftsZ"
                     /locus_tag="POH73_001768"
     CDS             3945..5117
                     /gene="ftsZ"
                     /locus_tag="POH73_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDI6887713.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
     gene            5375..6166
                     /gene="pgeF"
                     /locus_tag="POH73_001769"
     CDS             5375..6166
                     /gene="pgeF"
                     /locus_tag="POH73_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDI6887714.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDCPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LNNYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            6190..6857
                     /locus_tag="POH73_001770"
                     /pseudo
     CDS             6190..6857
                     /locus_tag="POH73_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
     gene            6854..7417
                     /locus_tag="POH73_001771"
     CDS             6854..7417
                     /locus_tag="POH73_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDI6887715.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            7429..7719
                     /locus_tag="POH73_001772"
     CDS             7429..7719
                     /locus_tag="POH73_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDI6887716.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            7829..8608
                     /locus_tag="POH73_001773"
     CDS             7829..8608
                     /locus_tag="POH73_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDI6887717.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFDLTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKIESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            8632..9228
                     /locus_tag="POH73_001774"
     CDS             8632..9228
                     /locus_tag="POH73_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDI6887718.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVASNTSDNNQQSNDSETTKK"
     gene            9449..12202
                     /gene="ileS"
                     /locus_tag="POH73_001775"
     CDS             9449..12202
                     /gene="ileS"
                     /locus_tag="POH73_001775"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDI6887719.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFNVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
BASE COUNT         4573 a         1726 c         2346 g         3657 t
ORIGIN      
        1 ttgaaaattt taacagaagt tgagttaagt tatctaatct ctgaagcacc aatcatagct
       61 gtaacaggta caaatggtaa aacgacagtt acttctctaa ttggagatat gtttaaaaaa
      121 agtcgcttaa ctggaagatt atccggcaat attggttatg ttgcatctaa agtagcacaa
      181 gaagtaaagc ctacagatta tttagttaca gagttgtcgt cattccagtt acttggaatc
      241 gaaaagtata aaccacacat tgctataatt actaacattt attcagcgca tctagattac
      301 catgaaaatt tagaaaacta tcaaaatgct aaaaagcaaa tatataaaaa tcaaacggaa
      361 gaagattatt tgatttgtaa ttatcatcaa agacaagtga tagagtcgga agaattaaaa
      421 gctaagacat tgtatttctc aacacaacaa gaagttgatg gtatttatat taaagatggt
      481 tttatcgttt ataaaggtgt tcgtattatt aacactgaag atttagtatt gcctggtgaa
      541 cataatttag aaaatatatt agcagctgtg cttgcttgta ttttagctgg tgtacctatt
      601 aaagcaatta ttgatagttt aactacattt tcaggaatag agcatagatt gcaatatgtt
      661 ggtactaata gaactaataa atattataat gattccaaag caacaaacac gctagcaaca
      721 cagtttgcct taaattcatt taatcaacca atcatttggt tatgtggtgg tttggatcga
      781 gggaatgaat ttgacgaact cattccttat atggaaaatg ttcgcgcgat ggttgtattc
      841 ggacaaacga aagctaagtt tgctaaacta ggtaatagtc aagggaaatc ggtcattgaa
      901 gcgaacaatg tcgaagacgc tgttgataaa gtacaagata ttatagaacc caatgatgtt
      961 gtattattgt cacctgcttg tgcaagttgg gatcaatata gtacttttga agagcgtgga
     1021 gagaaattta ttgaaagatt ccgtgcccat ttaccatctt attaaagggt gtgagtattg
     1081 atggatgata aaacgaagaa cgatcaacaa gaatcaaatg aagataaaga tgaattagaa
     1141 ttatttacga ggaatacatc taagaaaaga cggcaaagaa aaagatcaaa ggctacacat
     1201 ttttctaatc aaaataaaga tgatacatct caacaagctg attttgatga agaaatttac
     1261 ttgataaata aagacttcaa aaaagaacaa ggcaatgaag aaaataatga ttctgcttct
     1321 agtcatgcga atgataataa tatcgatgat tctacagact ctaatattga aaatgaggat
     1381 tatagatata atcaagaaac tgacgaccaa aatgaatcga atggaattgc agtcgacaac
     1441 gaacaacatc aatcagctcc aaatgaacaa aatagcgact cgaatgatga ggaaacagta
     1501 acgaaaaaag aacgaaaaag taaagtaaca caattaaaac cattaacact tgaagaaaag
     1561 cggaaggtaa gacgtaaacg acaaaaacga atccaataca gtgttattac aatattggta
     1621 ttgttgattg ctgttatatt aatttacatg ttttcaccac ttagtaaaat tgcgcatgta
     1681 aatataaatg gaaataatca cgttagtact tcaaagataa acaaagtttt aggtgttaaa
     1741 aatgattcga ggatgtatac gtttagtaaa aaaaatgcta ttaatgatct cgaagaggat
     1801 ccattaatca aaagtgttga gatacacaag caattaccaa acacattaaa cgtagatatc
     1861 acagaaaatg aaattattgc tttagtgaaa tataaaggta aatatttacc tttattagaa
     1921 aatggtaaat tgcttaaagg ttcaaatgat gtcaaaatta atgatgcacc tgtcatggat
     1981 ggtttcaaag gtacaaaaga agatgatatg attaaggcgt tatctgaaat gacacctgaa
     2041 gttagacgat atattgccga agtgacatac gccccaagta aaaacaaaca aagcagaatt
     2101 gaattgttta cgacggatgg acttcaagta atcggtgata tttcgacgat atctaagaaa
     2161 atgaaatatt atccgcagat gtcacaatca ttagcaagag atagttcggg taaactaaaa
     2221 acacgaggct atattgattt atcagtcggt gcttcattta tcccataccg tggaaacacg
     2281 tctactcaat cagaaagcga taaaaatgtg actaaatcat ctcaagagga aaatcaagca
     2341 aaagaagaat tacaaagcgt tttaaacaag attaacaaac aatcaagtaa gaataattaa
     2401 aaaattttga tattgtctat gtttatagtt cacaagccat tcaacgtatt gtaaactaag
     2461 gatagtgtat ttttttaata gtaatttgtc aggaggtgcc tatctatgga agaacattac
     2521 tacgtaagta ttgatattgg atcatcaagc gtaaaaacaa tagtaggcga gaaatttcac
     2581 aatggtataa atgtgatagg tacaggacaa acctacacga gcggtataaa aaatggttta
     2641 attgatgatt ttgatattgc gcgacaagca atcaaagaca caattaaaaa ggcatcaatc
     2701 gcttcgggtg ttgatattaa agaagttttc ctgaaattac ctatcattgg aacggaagtt
     2761 tatgatgaat caaatgaaat cgacttttat gaggatacag aaatcaacgg ttcacatatc
     2821 gaaaaagtat tagaaggtat tagagaaaaa aatgatgtgc aagaaacaga agtaattaat
     2881 gtattcccaa ttcgttttat agtcgataaa gaaaatgagg tttcagaccc taaagaatta
     2941 attgccagac attcattaaa ggttgaagca ggcgtaattg ctattcaaaa atcgatttta
     3001 attaatatga ttaaatgcgt tgaagcatgt ggtgttgatg tattagatgt ttattctgat
     3061 gcgtataact atggttcaat cctaacagct actgaaaaag agttaggtgc atgtgtcatt
     3121 gatattggtg aagacgttac gcaagttgct ttttatgaac gcggtgaatt agtagatgct
     3181 gattctatcg aaatggcagg gcgtgatatt acagacgata ttgcacaagg attaaacact
     3241 tcttatgaaa ctgctgaaaa agttaaacac caatacggtc atgcattcta tgattctgct
     3301 tcagatcaag atatcttcac tgttgaacag gttgatagtg atgaaacagt acagtatact
     3361 caaaaagatt tgagtgactt tattgaagcg cgtgtagaag aaatattctt cgaagtattt
     3421 gatgttttac aagatttagg attaacaaaa gtaaatggtg ggtttattgt aactggtgga
     3481 tctgcaaact tacttggcgt aaaagaatta ttatcagata tggtaagtga aaaagttaga
     3541 attcacacgc catcacaaat gggaattaga aaacctgaat tttcttcagc aatttctaca
     3601 atttctagta gtatcgcttt tgatgagtta ttagattatg ttacaattaa ttatcatgat
     3661 aatgaagaaa ctgaagaaga tgttattgat gtgaaagaca aagataacga atctaaatta
     3721 ggcggatttg attggtttaa acgtaaaaca aacaaaaaag atactcatga aaatgaagta
     3781 gagtcatcag atgaagaaat ttatcaatca gaagataatc atcaggaaca taaacagaat
     3841 catgaacatg ttcaagacaa agataaagaa gaaagtaaat tcaaaaaact aatgaaatct
     3901 ctatttgaat gattattggc caataaaact aggaggaaat ttaaatgtta gaatttgaac
     3961 aaggatttaa tcatttagcg actttaaagg tcattggtgt aggtggtggc ggtaacaacg
     4021 ccgtaaaccg aatgattgac catggaatga ataatgttga atttatcgct atcaacacag
     4081 acggtcaagc tttaaactta tctaaagctg aatctaaaat ccaaatcggt gaaaaattaa
     4141 cacgaggttt aggagcagga gctaaccctg aaatcggtaa aaaagctgca gaggaatctc
     4201 gtgaacaaat tgaagatgca atccaaggtg cagacatggt atttgttact tctggtatgg
     4261 gtggcggaac tggtactggt gcagcaccag tcgttgctaa aattgcaaaa gaaatgggcg
     4321 cattaactgt tggtgttgta actcgtccat ttagttttga aggacgtaaa cgtcaaactc
     4381 aagctgctgc tggagtagaa gctatgaaag ctgcagtaga tacattaatc gttataccaa
     4441 atgaccgttt attagatatc gttgacaaat ctacgccaat gatggaagca tttaaagaag
     4501 ctgataacgt gttacgccaa ggtgtacaag gtatctcaga cttaatcgct gtttctggtg
     4561 aagtaaactt agactttgca gacgttaaga caattatgtc taaccaaggt tctgcattaa
     4621 tgggtattgg tgtttcttct ggtgaaaata gagcggtaga agctgctaaa aaagcaatct
     4681 cttctccatt acttgaaaca tctatcgttg gtgcacaagg tgtgcttatg aatattactg
     4741 gtggcgagtc attgtcatta tttgaagcac aagaggctgc tgatattgtc caagatgctg
     4801 cagatgaaga cgttaatatg attttcggta cagttattaa tcctgaatta caagatgaga
     4861 ttgttgtaac agttattgca actggttttg atgacaaacc aacatcacat ggtcgtaaat
     4921 ctggtagcac tggattcgga acaagcgtaa atacttctag caatgcaact tctaaagatg
     4981 aatcattcac ttcaaattca tcaaatgcac aagcaactga tagtgtaagt gaaagaacac
     5041 atacaactaa agaagatgat attcctagct tcattagaaa tagagaagaa agacgttcaa
     5101 gaagaacaag acgttaatcg gttaatatat acacaaataa ttcaacacaa atcatcagat
     5161 aacatatctg atgatttttt tactaatttt tagaacatgt agaaggacat ttaagttttt
     5221 cgaagtcatt aaaagtgttt aagtatcgtg tcaaaattaa gtcgaaaatt atttgcgcaa
     5281 cattttaact ttaaacataa atgttatatt atataattat taactttgta cagttagacg
     5341 aagataattt aaatgaaatg atggtgacga tcgagtgaat gataatttta aaaagcaacc
     5401 gcatcattta atatatgaag aattattaca acaaggtatt actctaggta ttacaactag
     5461 aggcgatggt ttaagtgact atcctaaaaa tgcttttaat atggcgagat atattgatga
     5521 ttgcccatat aatattactc aacatcaatt gcaattagct gaagaaattg cgtttgatag
     5581 aaaaaattgg gtgtttccca tccaaacaca tgaaaataaa gtcgcttgta ttaccaagga
     5641 tgatataggc acaaatatag acactttaac tgatgcgctt catggtattg atgcgatgta
     5701 cacatatgat agtaatgtct tattaacgat gtgttatgca gactgtgtac cagtatattt
     5761 ttatagtaca aaacatcatt ttattgcatt ggcccatgca gggtggcgtg gtacctatac
     5821 tgaaattgta aaagaagtgc taaaacatgt gaactttgat ttgaaagact tacatgtcgt
     5881 tattggacca tctacatcat caagttatga aattaatgat gatattaaaa ataaatttga
     5941 aacattgcca attgatagtg ccaactatat tgaaactaga ggacgagatc gtcatggtat
     6001 tgatttgaaa aaagccaatg ctgcattatt aaataattat ggtgttccta aagaaaatat
     6061 ttatacgaca gcgtatgcta catctgaaca tttagagtta tttttctctt atcgattaga
     6121 aaaaggtcaa acaggacgca tgttagcatt cattggtcaa cagtaaacaa ggaggagata
     6181 tgtttgcgtg tgaaagataa tttacaacaa atctcaacac aaattaatga caaaagtgaa
     6241 aaaataattt ttcaacaaaa ccaaacgtga ttgcagttac aaaatatgtt acaatagagc
     6301 gagctaaaga agcgtatgag gctggaataa gacattttgg tgagaataga ttggaaggct
     6361 tttttcaaaa gaaagaagca ttaccatcag atgcggtgat ccattttata ggatcattac
     6421 aatctcgaaa agttaaggac gttataaacg acgtagatta tttccatgct ttagatcgat
     6481 tgagcttagc caaagaaatt aacaaacgtg cagaacataa aattaaatgt ttcttgcaag
     6541 tgaacgtttc gggagaagct tctaagcatg gtattgcttt agaagatgtt gatcagttta
     6601 tagatgatct taaaaaatat gacaaaatcg aaattgtagg tttaatgacg atggcaccat
     6661 tgacagatga tgaagcatat attagatcgt tatttaaaca gttacgtttg aaaaaagaag
     6721 aaatacaacg actcaattta gaatatgcgc cttgtgatga attatcaatg ggaatgagta
     6781 atgactatct tattgcagtt gaagaaggtg caacgtttgt tagaattggg actaaacttg
     6841 taggagaaga ggagtgagcc acttggcttt aaaagattta tttagtggat tttttgtaat
     6901 agatgatgaa gaggaagtag aagtacctga caaacaacaa caggtaaatg aagcgccagc
     6961 aaaagagcag tcacaacaaa caacaaaaca aaacgcaatc aaatcagtcc ctcaaaaatc
     7021 tgcatcaaga tatacaacaa cgtcagaaga aaggaataac cgtatgtcta attattcaaa
     7081 aaataattca cgtaatgttg taactatgaa caatgctaca ccaaacaatg catcacaaga
     7141 aagttcaaaa atgtgtttat tcgaaccacg tgttttttca gatacacaag atattgctga
     7201 tgagcttaaa aaccgccgtg cgacacttgt caatttacaa cgtattgata aagtatcagc
     7261 gaaaagaatt attgattttt taagcggtac tgtttatgca atcggtggag atatccaacg
     7321 tgtaggtact gatattttct tatgtacgcc tgataatgtg gaagtagctg gaagcattac
     7381 agaccatatt gaaaatatgg aacattcatt cgactaagga gttaacatat ggatataaat
     7441 gtgctagcta caatttttaa atttatcctt tttgttgttg aaatttatta tttcggcatg
     7501 attatatatt tctttacatc ttgggtacca agtattagag aaactaaggt aggttatttt
     7561 ttagcgaaaa tatatgaacc tttcttacaa ccatttagaa aagtaattcc acctattgga
     7621 attatcgaca tatcatcaat cgctgcaatt atcgttttag tattattcca aaaagggtta
     7681 ctccaaatct ttaattggat tttaattcaa ttacaataaa ttaagcgaca taacgaggca
     7741 agggtacatg atactttagc ctcgtttttg atatgtattt ttctaaatat aagggcaata
     7801 gatggtattt tataattttt ttaaggtagt gattaacata gatatttatc aacactttag
     7861 acaggaagaa tacgaattaa ttgatcagct aacggataaa tgtgatcaag cggaacagca
     7921 ttatgcacca gtattaacgc attttttaga tccaagaggg caatatatat tggaagtgat
     7981 ttgtggcagt tatgaagatt taaacgtatc tttttatggc gggcctaatg ctgaaagaaa
     8041 aagagcaatc atttcgccga actattacga acctaaagaa agtgactttg atttaacttt
     8101 aatggaaata gattatcctg aaaaattcgt cactttaaaa catcaacata ttttagggac
     8161 attaatgtct ttaggtatcg aacgcgaaca agttggagat ataattgtga atgaacgaat
     8221 tcaatttgtt ttgacaagta gattggaatc atttattatg ttagaattac aacgtattaa
     8281 aggcgcatca gttaaacttt atactattcc agtaacagat atgatacaat ctaatgagaa
     8341 ttggaaaatt gaaagtgcaa cagttagttc tttaaggtta gatgttgtta ttaaagaaat
     8401 gatacgtaaa tcacgtacga ttgcgaaaca actaatcgaa aaaaaacgtg ttaaagtgaa
     8461 tcacactatt gttgattcag cagattttca attacaagca aatgatttaa tatccatcca
     8521 aggttttggt agagcacaca ttactgactt aggtggtaaa actaaaaaag ataaaacgca
     8581 cattacctat agaacattat tcaaatagta atgatttaag gaggataaca aatgcctttt
     8641 acaccaaatg aaattaagaa taaagagttt tcacgtgtaa agaatggttt agaacctact
     8701 gaagttgcta attttttgga gcaactaagc actgaaattg aacgtcttaa agaagataaa
     8761 aaacaacttg aaaaagtaat cgaagagaga gatactaata ttaagtctta tcaagacgtg
     8821 catcaatctg taagtgatgc tttgatacaa gctcaaaaag ctggtgaaga aactaagcaa
     8881 gctgcagaga aacaagctga agcgattata gctaaggcag aagcacaagc taatcaaatg
     8941 gttggtgacg cggtagaaaa agcacgccgt ttagcattcc agactgaaga tatgaaacgt
     9001 caatcaaaag tatttagatc gcgtttccgt atgttagttg aagcgcaatt agacttatta
     9061 aaaaacgaag attgggatta cttgttgaat tatgatttag acgctgaaca agtgacgctt
     9121 gaaaatattc atcatttgca tgaaaatgat ttaaagccag atgaagttgc atcaaataca
     9181 tcagacaata atcaacaatc caatgattca gaaacaacta agaagtaaga attaaataaa
     9241 gacagacgcg taatatacat ttaacttttc acagcgaatt aggtaatggt gagagcctag
     9301 taaaagcatg tatgttatat cactggcttt ttaatattta aataatgtaa tgagagaact
     9361 ctaagttgag ttaataaggg tggtaccgcg agcaatcgtc ccttttaatt taacttagag
     9421 ttttttaaat ttttaaggag tgaaaaaaat ggattacaaa gaaacgttat taatgcctaa
     9481 aacagatttc ccaatgcgag gtggtttacc aaacaaggaa ccgcaaattc aagaaaaatg
     9541 ggatgcagaa gatcaatacc ataaagcgtt agaaaaaaat aaaggtaacg aaacattcat
     9601 tttacatgat ggcccaccat acgcgaatgg taacttacat atgggacatg ccttgaacaa
     9661 aattttaaaa gactttattg tacgttataa aactatgcaa gggttctatg caccatacgt
     9721 accaggttgg gatacacatg gtttgccaat tgaacaagca ttaacgaaaa aaggtgttga
     9781 ccgtaagaaa atgtcaacag ctgaattccg tgagaaatgt aaagaatttg ctttagaaca
     9841 aattgaatta cagaaaaaag attttagacg tttaggtgtt cgtggtgact ttaatgatcc
     9901 atatattaca ttaaaacctg aatacgaagc tgcacaaatt cgtatttttg gagaaatggc
     9961 agataaaggt ttaatttata aaggtaaaaa gccagtttat tggtctcctt caagtgagtc
    10021 ttcattagca gaagcagaaa ttgaatatca cgataaacgt tcagcatcaa tttacgttgc
    10081 atttaacgtt aaagatgaca aaggtgtcgt tgatgcagat gctaaattta ttatctggac
    10141 aacaacgcca tggacaattc catcaaatgt tgcgattacc gttcatccag aattaaaata
    10201 cggtcaatac aatgtaaatg gcgaaaaata tattattgca gaagccttat ctgacgccgt
    10261 agcagaagca ctggattggg ataaagcatc aatcaaatta gaaaaagaat acacaggtaa
    10321 ggaattggag tatgttgtag cacaacatcc attcttagat agagaatcgt tagtgattaa
    10381 tggtgatcat gttactacag atgctggtac aggttgtgta catacagcac caggtcacgg
    10441 ggaagatgac tatattgttg gtcaaaaata tgaattgcca gtaattagtc caatcgatga
    10501 taaaggtgta tttactgaag aaggcggcca atttgaagga atgttctatg ataaagctaa
    10561 taaagccgtt actgatttat taacagaaaa aggtgcacta ttaaaattag actttattac
    10621 acatagctat ccacacgact ggagaacaaa aaaacctgta attttccgtg ctacaccaca
    10681 atggtttgct tcaatcagta aagtaagaca agatatttta gatgcaatcg aaaatacaaa
    10741 cttcaaagta aattggggta aaacacgtat ttacaatatg gttcgtgacc gtggcgaatg
    10801 ggttatttct cgtcaacgtg tttggggtgt accgttacca gtattttatg ctgaaaatgg
    10861 cgaaattatc atgacgaaag aaacagtgaa tcatgttgct gatttatttg cagaacacgg
    10921 ttcaaatatt tggtttgaaa gagaagcgaa agacttacta ccagaaggat ttacacatcc
    10981 aggcagccct aacggtacat ttactaaaga aacagacatt atggacgttt ggtttgattc
    11041 tggttcatca caccgtggcg tgttggaaac aagaccggaa ttaagtttcc cagcagatat
    11101 gtatttagaa ggtagtgacc aatatcgtgg ttggttcaac tcttctatta caacttcagt
    11161 tgctacaaga ggagtatcac cttataaatt cttactttct catggttttg ttatggacgg
    11221 tgaaggtaag aaaatgagta aatctttagg taatgtgatt gtacctgacc aagtggttaa
    11281 acaaaaaggt gctgatattg cgagactttg ggtaagtagt acggactatt tagctgatgt
    11341 tagaatttct gatgaaattt taaaacaaac atctgatgtt tatcgtaaaa tcagaaatac
    11401 attaagattt atgttaggta atattaatga tttcaatcct gatacagata gcattcctga
    11461 atcagagtta ttagaagttg atcgttactt gctaaatcgt ttacgtgaat ttactgcaag
    11521 tacgattaac aactatgaaa actttgacta cttaaatatt tatcaagaag ttcaaaactt
    11581 tatcaatgtt gagttaagta atttctattt ggattacggt aaagatattt tatatattga
    11641 acaacgtgat tctcatatcc gtcgtagtat gcaaacagtg ttatatcaaa ttttagttga
    11701 tatgacgaag ttgttagcac caatcttagt gcatacagct gaagaagttt ggtctcatac
    11761 accacatgtt aaagaagaaa gtgttcactt agcagacatg cctaaagttg tagaagtaga
    11821 tcaagcttta ttggataaat ggcgtacatt tatgaattta cgtgatgatg tgaaccgtgc
    11881 attagaaact gctcgtaatg aaaaagttat tggtaaatca ttagaagcta aagttacgat
    11941 tgctagtaac gataaattta atgcatctga attcttaact tcatttgatg cattacatca
    12001 attatttatc gtgtcacaag ttaaagttgt agataagtta gatgatcagg caacagctta
    12061 tgaacatggt gatattgtca tcgaacatgc agatggtgaa aaatgtgaaa gatgttggaa
    12121 ctattcagag gatcttggtg ctgttgatga attgacgcat ctatgcccac gatgccaaca
    12181 agttgtaaaa tcacttgtat aattgaaatt gtataaagta cttatacaga tgatataaat
    12241 taaagctctc ttcataatca tgttgtagtt tttgttgaca tgatgaagag agtttttttg
    12301 ag
//
LOCUS       DAMZPP010000085        12181 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMZPP010000085 DAMZPP010000000
VERSION     DAMZPP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12181
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.085"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..133
                     /locus_tag="POH73_001776"
     CDS             <1..133
                     /locus_tag="POH73_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDI6887720.1"
                     /translation="TDNKNLENEGKEDKASGKAKEFVENAKEKATDFIDKVKGNKGE"
     gene            complement(367..1218)
                     /locus_tag="POH73_001777"
     CDS             complement(367..1218)
                     /locus_tag="POH73_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDI6887721.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNSDKHAGETLTLSTGLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
     gene            1546..2307
                     /gene="sufC"
                     /locus_tag="POH73_001778"
     CDS             1546..2307
                     /gene="sufC"
                     /locus_tag="POH73_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDI6887722.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            2405..3712
                     /gene="sufD"
                     /locus_tag="POH73_001779"
     CDS             2405..3712
                     /gene="sufD"
                     /locus_tag="POH73_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDI6887723.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            3818..5068
                     /locus_tag="POH73_001780"
     CDS             3818..5068
                     /locus_tag="POH73_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDI6887724.1"
                     /translation="MNEVAEHSFDVNELIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            5058..5522
                     /locus_tag="POH73_001781"
     CDS             5058..5522
                     /locus_tag="POH73_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDI6887725.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            5673..7070
                     /gene="sufB"
                     /locus_tag="POH73_001782"
     CDS             5673..7070
                     /gene="sufB"
                     /locus_tag="POH73_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDI6887726.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIELFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            7257..7396
                     /locus_tag="POH73_001783"
                     /pseudo
     CDS             7257..7396
                     /locus_tag="POH73_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7402..7716)
                     /locus_tag="POH73_001784"
     CDS             complement(7402..7716)
                     /locus_tag="POH73_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887727.1"
                     /translation="MYFVLAIFTLISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSKDVFKTYGPLATSVVNLILLIVF
                     LF"
     gene            8085..9125
                     /locus_tag="POH73_001785"
     CDS             8085..9125
                     /locus_tag="POH73_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDI6887728.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKEDQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            9139..10206
                     /locus_tag="POH73_001786"
     CDS             9139..10206
                     /locus_tag="POH73_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDI6887729.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPNESIIKRLKEANIKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVVTKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELTHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            10506..11354
                     /locus_tag="POH73_001787"
     CDS             10506..11354
                     /locus_tag="POH73_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDI6887730.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            11367..>12181
                     /locus_tag="POH73_001788"
     CDS             11367..>12181
                     /locus_tag="POH73_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDI6887731.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQIAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKTFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIR"
BASE COUNT         4456 a         1755 c         2262 g         3708 t
ORIGIN      
        1 tactgataat aaaaatttag aaaacgaagg taaagaagat aaagcttctg gtaaagcgaa
       61 agaattcgtt gaaaatgcaa aagaaaaagc aactgatttt attgataaag taaaaggtaa
      121 caaaggagag taattttact ttatattcct aatacactgc acatgccact tagtcggtat
      181 aaccatcggc taggtggttt tttgtattaa aaaagtggat accaaaattt attaataatt
      241 attttaatgt tagaaaaaaa ctaaataaaa actcgttaat gatatccaat aatatgtata
      301 caaaacgaga catatattgc atatgattaa cgagatactg aatatatttt atcaccccta
      361 aaatgattat tcattttcag cggtaattcg acctaaagtc aaacttacaa taaaaccgat
      421 gataaatact actaatgaaa cgaaccacat cacgatatta gttggtaaac ctggaaatac
      481 tgcaaagagg gagccaacaa caaaaccaat gattaatgca aaagtcatta gtttatgatg
      541 tgttaggaaa tactggataa ttttgcttga aataatgaat ccagcaagca cgccaaatcc
      601 gactgcaagt aatataggaa gaccggcaaa gttaagttta acaacttcag atattgctag
      661 catgaccgta ccatagacgc caaatactaa taacataaat gaccctgaaa tacctgggag
      721 taacatagca ctagatgcac acatacctgc aataaagtat ttaataataa gaccagttga
      781 tagagtaagt gtttctccag catgtttatc gctattattc attaatgtaa taacaattaa
      841 gatagcgata ccagctataa ccatcatgta atgtttagtt gtaaatgacg ttttatagtt
      901 agaaattttc aataaatatg gaacgatacc aatgattaat ccaccaaaga aaaacatagt
      961 tggaatatgg tgttggctta ataaataatt aaaaagatta cttagtgatc ccattgccag
     1021 taacattcca attataatag ggattaaaaa tgtaaaactt ggccaaaaac gtcgtgagaa
     1081 tatgccacta attgaagcga taaattgatt gtatatacct aacaataatg caatagtccc
     1141 accgctaaca ccaggtacca agtcactcgt tcccatagca aaacctttta gaatattaat
     1201 ccatttaaat tgttgcatga ataactcctt tcaaacgatt ggaataaaat cataaatagc
     1261 atcataccat attacaaatg tcctagtgaa atgataacat attttaaatt cataaaatcc
     1321 attgagaaat tatgtgttct tattatcatt tatattttta aagagagcgg attagagttg
     1381 tactttagag ttattaaaaa taataaaaag ggtttaagtt gtttatatta aggtataagt
     1441 aagttataat taactgaacg cattattaca aagtcttttt gactacaaat taaaattatt
     1501 ataaactagt taagaaaact ttatatttta cggagggaat ataaaatggc atcaacatta
     1561 gaaatcaaag acctacatgt gtctattgag gataaagaaa tcttaaaagg tgttaacttg
     1621 acaattaaca ctgatgaaat acatgcgatt atgggaccaa acgggacagg taaatcaact
     1681 ttgtcatctg caattatggg acacccaagt tatgaagtaa ctaaaggaga agtactttta
     1741 gacggtgtaa atattttaga attagaagtt gatgaaagag caaaagcagg attattcttg
     1801 gcaatgcaat atccatcaga aattacaggt gttacaaatg ctgatttcat gcgttcagct
     1861 atcaatgcga aacgtgaaga aggacaagaa atcaacttaa tgcaatttat taagaaatta
     1921 gataaaaata tggattttct agacatagat aaagacatgg cacaacgtta tttaaatgaa
     1981 ggtttctcag gtggagagaa gaaacgtaac gaaatcttac aattaatgat gttagaacct
     2041 aagtttgcaa tcttagatga aatcgattca gggttagaca tcgatgcatt aaaagttgta
     2101 tctaaaggta ttaaccaaat gcgtggggaa aactttggtg cattaatgat cacacactat
     2161 caacgattat taaattacat tactcctgat aaagtacatg taatgtatgc tggtaaggtc
     2221 gttaaatctg gtggtccaga attagcaaaa cgtcttgaag aagaaggata tgaatgggtt
     2281 aaagaagagt tcggttcagc tgaataatct tattaataca gtatccatga gatgttcatc
     2341 tatatatgat gaaaatgaac atttatacga aatagtaaat ttcatcaagt aggaggaaaa
     2401 agttatgaca actgatattt tgaacatttc tgaagaacaa cttgttgatt attctaaagc
     2461 ccacaatgaa ccttcttgga tgacagaatt acgtaaaaaa gctttgaaat taacagaaac
     2521 tttagaaatg ccaaaacctg ataaaacaaa attaagaaaa tgggattttg attcttttaa
     2581 acaacacgat gtaaaaggtg atgtttatca atctttatca caattacctg agtcagtaag
     2641 agaaattatt gacgtagatc attctaaaaa cttagtaatt caacataata atacgattgc
     2701 gtacacacaa gttgatgata atgcatcgaa agatggcgtt atcgttgaag gtttagcaga
     2761 tgctcttatg aaccatagtg atttagtaca aaagtacttt atgaaagatg cagtaacagt
     2821 agatgaacat cgtatcacag cgctacacac ggcattagtt aatggtggcg tatttgttta
     2881 tgttcctaaa aatgtagttg tagaacatcc agtacaatac gttgtgttgc acgacgacga
     2941 aaatgcaagc ttttataacc atgtcatcat cgttactgaa gaaagcgccg aagtcacata
     3001 tgttgaaaat tacttatcaa atgcatctgg tgaaggaaat caattaaata ttatttctga
     3061 agtgattgct ggtgcaaatt caaatatcac atatggctca gtagactata tggataaagg
     3121 ctttacaggt catatcattc gacgtggtat tactgaagcg gatgcctcaa ttaattgggc
     3181 attaggttta atgaatgagg gtagccaaat tattgataat acaacaaatt tatttggtga
     3241 tcgttcaaca agttcactta aatcagtagt tgtaggtaca ggcgaacaaa aaattaatct
     3301 aacatctaaa atcgtacaat atggtaaaga aactgatggt tatatcctta aacatggtgt
     3361 tatgaaagaa catgcatcat ctgtatttaa tggtatcggc tacattaagc atggtggaac
     3421 taaatcaatt gctaatcagg aatcacgtgt attaatgtta tctgaacatg ctcgtggtga
     3481 cgcgaatcct attttattaa ttgatgaaga tgatgtacaa gctggtcatg ctgcatcagt
     3541 aggtcgtgtt gatccagatc aactttacta tttaatgagt cgtgggattt ctcaaagaga
     3601 agcggaacgt cttgttatac atggtttctt agatccagta gtacgtgaat tacctatcga
     3661 agacgttaaa cgtcaattga gagaagtaat tgaacgcaaa gtttctaaat aatattttga
     3721 aaataaaagt ttgtaataga tatagactgt cgatattggt ataagactaa tacaacgtca
     3781 gtatttaaat gattaggatt tttatttaag aaaggtcgtg aatgaagtgg ccgaacactc
     3841 atttgacgtt aatgaattaa tcaaggattt tccgatatta gatcaaaaag tcaatggcaa
     3901 acgtttagca tatcttgatt caacagcgac aagtcaaacg cctgtgcaag tgttaaatgt
     3961 tttagaagat tactacaagc gttataattc aaacgttcat cgtggtgttc atacattagg
     4021 atcattagca actgatggtt atgaaaatgc ccgtgaaacc gttcgtcgtt ttattaatgc
     4081 gaagtatttt gaagaaatca tttttacacg cggaacaact gcgtcgatta accttgtagc
     4141 acatagctat ggtgatgcaa atgttgaaga gggcgatgaa attgttgtca ctgaaatgga
     4201 acatcatgcc aatattgttc cttggcaaca gttagcaaag cgtaaaaatg cgacattgaa
     4261 atttatacca atgacagctg acggtgaatt aaacatcgag gatattaagc aaacgattaa
     4321 tgataaaaca aagatcgttg ctattgcaca tatttctaat gtactcggta caattaatga
     4381 tgttaaaacc attgcagaaa tagctcatca acatggtgca attatcagtg ttgatggggc
     4441 gcaagcagca ccacatatga aacttgatat gcaagaaatg aatgctgatt tttatagttt
     4501 tagtggacat aaaatgcttg gaccaacagg tattggcgta ttatttggta aacgtgagtt
     4561 actacaaaaa atggaaccga ttgagttcgg tggcgacatg attgattttg taagtaagta
     4621 tgatgcaaca tgggctgatt tacctactaa atttgaggcg ggtactccat taattgctca
     4681 agcaattggg cttgcagaag ctattcgcta tttagaacgc ataggttttg atgcaattca
     4741 taaatatgaa caagaattaa cgatatatgc ttatgagcaa atgtctgcaa ttgaaggaat
     4801 tgaaatttat ggcccgccaa aggatcgtcg tgcaggtgta ataacgttta atttacaaga
     4861 tgtacatcca cacgatgttg ctacagccgt agatacagaa ggtgtagcgg ttagagctgg
     4921 gcatcattgt gcgcaaccgt taatgaaatg gttaaatgtg tcttcaacag ctagagcgag
     4981 tttttatata tacaacacga aagaagacgt tgatcagtta attaatgcct tgaaacaaac
     5041 gaaggagttt ttctcttatg aattttaata atctagatca attatataga tctgtcatta
     5101 tggatcatta taaaaatcct agaaataaag gtgtattaga taacgggtct atgacagtag
     5161 atatgaataa cccgacatgc ggtgaccgta tacgactaac atttgatata gaagacggca
     5221 ttataaaaga tgctaagttt gaaggtgaag gttgttcgat ttcaatggca agtgcatcga
     5281 tgatgacaca agccgttaaa ggtcattcac ttggagaagc aatgcaaatg agccaagaat
     5341 ttacgaaaat gatgcttggt gaagactatg tgattacaga agaaatggga gatattgaag
     5401 cattgcaagg tgtatctcaa ttcccagctc gtattaaatg tgccacatta gcttggaaag
     5461 cattggaaaa aggtactgtt gctaaagaag gtaaagcaga aggtacgact gaagaagaat
     5521 agaatgctgt taatcataga tgattttgat attagacata taatagtaaa aaaattttta
     5581 tatgatgtca tgtcattgtt ataatatggt ttacatcatg aattaaaaac ttacgcacgc
     5641 cgttgtaaat atatttttaa ggagtgattg aaatggctaa aaaagcacct gatgttgggg
     5701 attataaata tggattccac gacgatgatg tgtccatttt cagatcagaa cgtggtttaa
     5761 ctgagaatat cgttagagaa atttctaaca tgaaaaatga gccggaatgg atgttagatt
     5821 tccgtcttaa atcattaaaa ttgttttata aaatgccaat gcctcaatgg ggtggcgact
     5881 tatcagaatt gaatttcgat gacattactt actatgtaaa gccttcagaa caagctgaac
     5941 gttcatggga tgaagtgcct gaagaaatta aaagaacttt cgataaatta ggaattcctg
     6001 aagctgaaca aaaatattta gctggtgttt ctgctcaata tgaatctgaa gttgtttacc
     6061 ataatatgga aaaagaactt gaagaaaaag gtattatctt taaagataca gatagtgctt
     6121 tacaagaaaa tgaagaatta ttcaaaaaat actttgcttc tgtagtacct gcagcagata
     6181 acaaatttgc ggcgttaaac tcagcagtat ggtcaggtgg ttcgttcatt tatgtaccta
     6241 aaaatatcaa actagatacg ccactacaag cttatttccg tattaactct gagaacatgg
     6301 gtcaatttga acgtacatta atcattgctg atgaaggtgc ttctgtacat tacgtagaag
     6361 gttgtactgc accagtttat acaactagtt ctttacactc tgctgttgtg gaaatcattg
     6421 tgcataaaga tgcgcacgtt cgttatacta cgattcaaaa ctgggcgaac aatgtataca
     6481 atttagttac gaaacgtact tttgtttatg aaaacggaaa tatggaatgg gtagatggta
     6541 acttaggttc taagttaacg atgaaatatc caaactgtgt tcttttaggt gaaggtgcaa
     6601 aaggtagtac attatctatt gcatttgctg gtaaaggaca agttcaagat gccggtgcta
     6661 aaatgattca taaagcgcca aatacatctt caacgattgt ttctaaatct atttctaaaa
     6721 atggtggtaa agttatttat cgcggtattg ttcattttgg acgtaaagca aaaggcgctc
     6781 gttcaaatat tgaatgtgat acattaatct tagataacga atcaacatca gatacaattc
     6841 catataacga agtattcaac gatcaaatat cattagaaca tgaagcgaag gtttcaaaag
     6901 tttctgaaga acaattattc tatctaatga gtcgtggtat ttctgaagaa gaagcgacag
     6961 aaatgattgt tatgggattc atcgaactat ttacaaaaga acttccaatg gaatacgcgg
     7021 tcgaaatgaa ccgtttaatc aagttcgaaa tggaaggtag tattgggtag ctttaaaccg
     7081 cgttgttaag ccattcttaa ctgtcgaaaa cggttattga taccattttg atactgaata
     7141 taacaaaaag ccacattact gtggcttttt ttgttttata actaaatcgg attgatagat
     7201 aagctttgta cttatttata tcagtccgat tttttgattg gtgtaaaaaa taatcattga
     7261 tgctagataa agcgacaaca taaatacaac atgattgtgg cattagagtg ctggtcttta
     7321 ttaaattaat tgaaaactac atcaaatatc ctttaaagat aattcgataa tagttcgatt
     7381 aagtttcgtt gtataagtga gttaaaataa gaaaactatt aataaaatta agttcactac
     7441 agatgttgct aatggaccgt aagttttaaa gacatcttta cttttataac caacaatcgc
     7501 atctaaaaat tgaactaaaa tcattgcaat ggatatagtt atcaaaaata tagcactatg
     7561 aatgactaaa gaaaaaatag ctaataaaaa taaaggtaag cttcgactaa gtgcataata
     7621 tgcatttata ttatggctag atgcacaagc ttgaattgaa taacctaaac tcacactggc
     7681 actaatgagt gtaaatattg ctaaaacaaa atacatgtta atccttcttt ctatatttga
     7741 atatctgtaa acaagtactt gtctaaagat atttaaaaga taattagaat aaatttatga
     7801 gaaacttttt gttatcatta tagcgatttc aaatcattat aactatgtca taaactaaat
     7861 ttgttaaaat ttttcattat gtaaatttat taataacaat taggtcgaat tatagcatca
     7921 ttttactaat aaatacatta aagtagctat gattaaaaat gcatattaat tatcattatt
     7981 aagtctatta tatataatga attttaactg gtttattaaa cgagaacgtc gggaattagg
     8041 taactacaat aaaaataaga tatgacaata aggagactac acgcgtgatc attgccataa
     8101 ttatattgat atttatttcg tttttctttt caggaagcga gacggcatta acggctgcca
     8161 ataaaacaaa atttaaaact gaagctgaca aaggtgataa aaaagcaaaa ggcattgtaa
     8221 agttacttga aaaaccaagt gagtttatta caactattct aattgggaat aatgtcgcga
     8281 atattttatt accaacactt gttacaatta tggctttacg ttgggggatt agcgttggga
     8341 ttgcatcagc tgttttaaca gttgttatca ttttgatttc cgaagtgatt cccaagtctg
     8401 tcgctgcgac atttccagat aaaataacaa ggcttgtata tccaattata aatatttgtg
     8461 tcattgtgtt ccgtcctatc acattacttt taaataagtt gacggacagt attaatcgaa
     8521 gtttatctaa gggtcaacct caagaacatc aattttcaaa agaagaattt aaaacaatgt
     8581 tagcaattgc tggacatgaa ggtgctttaa atgaaattga gacgagtagg ttggaaggtg
     8641 tcattaattt tgaaaattta aaagtaaaag atgttgatac aacacctaga attaatgtga
     8701 cggcatttgc ttcaaatgcg acatacgaag aagtttatga aacggttatg aataagccat
     8761 acactagata tccagtgtac gagggagata ttgataacat tattggggtg tttcattcta
     8821 aatatctgtt ggcttggagt aataaaaaag aagatcaaat tacaaactat tcagctaagc
     8881 cattatttgt gaatgaacac aataaagctg aatgggtatt acgtaagatg actatttcta
     8941 gaaaacattt agcaattgtg ttggacgaat ttggtggtac tgaagcgata gtgtcacatg
     9001 aagacttaat tgaagaatta ttaggtatgg aaattgaaga tgagatggat aaaaaggaaa
     9061 aagaaaaact ttctcaacag caaattcaat ttcaacaacg gaaaaatcgc aacgtatcta
     9121 tataaggagc gaacagctat gtggaataag aaccgactta ctcaaatgtt aagtattgaa
     9181 tatccaatta tacaagcagg tatggcagga agtacgacac cgaaattagt tgcatcagta
     9241 agtaacagtg gtggattagg cacaataggc gcaggttact ttaatacgca gcaattagaa
     9301 gacgaaatag attatgtacg ccaattaacg tcaaattctt ttggtgtaaa tgtctttgta
     9361 ccaagtcaac aatcatatac cagtagtcaa attgaaaata tgaatgcatg gttaaaacct
     9421 tatcgacgcg cattacattt agaagagccg gttgtaaaaa ttaccgaaga acaacaattt
     9481 aagtgtcata ttgatacgat aattaaaaag caagtgcctg tatgttgttt tacttttgga
     9541 attccaaatg aatctattat aaaaagattg aaagaagcaa acattaagct aataggtaca
     9601 gcaacaagtg ttgatgaagc tattgcgaat gaaaaagcag gtatggatgc tatcgttgct
     9661 caaggtagtg aagcaggtgg acatcgtggt tcatttttaa aacctaaaaa tcaattacct
     9721 atggttggaa caatatcttt agtgccacaa attgtagatg tcgtttcaat tccggtcatt
     9781 gccgctggtg gaattatgga cggtagagga gttttggcaa gtattgtctt aggtgcagaa
     9841 ggggtacaaa tgggcaccgc atttttaaca tcacaagata gtaatgcatc agaactactg
     9901 cgagatgcaa ttataaatag taaagaaaca gatacagtcg ttacaaaagc gtttagtgga
     9961 aagcttgcac gcggtatcaa caataggttt atcgaagaaa tgtcccaata cgaaggcgac
    10021 atcccagatt atccaataca aaatgagcta acaagtagca taagaaaagc cgcagcaaac
    10081 atcggcgaca aagagttaac acatatgtgg agtggacaaa gcccgcgact agcaacaacg
    10141 catcccgcca acaccatcat gtccaatata atcaatcaaa ttaatcaaat catgcaatat
    10201 aaataatcga ccgcaatcca caaaagcaca agcaccccca aacattattt tagtgcttgt
    10261 catttttgtg gattgcgttt ctattttacc aatctagtca aacgaaatta tcgagctaaa
    10321 gttcgctgaa atgtttatga tcaaacgaaa tcatcaagct aaagttcact gaaatgatta
    10381 tgatcaaacg aaattatcga gctaaagttc actgaaatga ttataatcta acgaaaacat
    10441 caaactaaag ttcgctgaaa tgattatgat aaaagttata tggtatgatg acattggtga
    10501 tatatatgat aaacatcgga ttaacaggtt ggggtgatca ctattcatta tatgaagatt
    10561 tagaacgcca aaccgataaa cttaaaacat atgctggaca ttttccggtt gtcgaattag
    10621 atgcgacata ctatgcgata caaccggaaa gaaatatatt gaaatggata aaagaaacgc
    10681 ctgatacatt tgaatttgtg gtcaaaattc atcaagcact cacattgcat gcagactaca
    10741 aaacatttgc agatacaagg caagaactat ttgatcaatt taaaaatatg ttagagccct
    10801 tacatacaca gaaaaaatta gcaatggtat tagttcaatt tcctccgtgg tttgactgca
    10861 atgcacaaaa tatcaaatat attttgtatg taagacagca attacaagca tttccaatgt
    10921 gtgtagaatt taggcatcaa tcatggttta gtgatgcatt taaagaacaa acattggcat
    10981 ttttaacaga acatcagatc attcatgcag tagttgatga accacaagtg aaagatggca
    11041 gtgtaccttt agtcaatcga atcacaaatg aaattgcgtt tgtacgttat catggacgta
    11101 atcattatgg ttggactaag aaagatatgt cagatcaaga atggcgagat gtacgctatt
    11161 tatatgatta taatgagcaa gaattaatag acttagcaca aaaggcacaa atattagcgc
    11221 aaaaagctaa gaaagtttac gtcatattta acaataattc tggtggtcat gcagcaaata
    11281 atgccaaaac atatcagcga ttattgaata tagaatatga agggttagca ccacaacaat
    11341 taaaattatt ttaagaggcg acgactatgt tattaacaat tacattatta gttctaatcg
    11401 gtggtttgtc agcgattata gggtcaatcg taggcattgg aggcggtatt attatcgttc
    11461 caacaatggt ttacctcggt gttgaacatg gattactaca taatattaca acgcaaatag
    11521 cgattgggac atcgtctgtc attctaattg tgacaggact ttcttcatcg cttggatatc
    11581 taaaaacaaa acaagttgat attaaaaatg gctctatctt tttatttgga ttattaccag
    11641 gttcattgct tgggtcattc attagtagat atttaacatt tgagtcattt aatttatatt
    11701 ttggaatctt tttaatcttt gtagctattt tattaatggt aagaaataag attaaaccgt
    11761 ttaaaacatt tgataaacca aagtatgaaa aaacttatgt agacgctaaa ggtaaaacat
    11821 atcattatag tgtgccacca ttgtttgctt ttattacaac gtttttaatt ggtatattga
    11881 caggtttatt tggtattgga ggtggcgcac taatgacgcc actaatgctt attgtattta
    11941 gatttccacc tcatgtagct gttggaacaa gtatgatgat gattttcttt tcaagtgtca
    12001 tgagttctat agggcacatt gctcaaggtc acgtagcttg gggttatgca atcattttaa
    12061 ttatatctag ttattttggt gcgaaaatcg gtgtcaaagt gaatcaatca attaaatcag
    12121 atacggtagt aacattattg agaacagtaa tgttgttaat gggtatatat ttaattattc
    12181 g
//
LOCUS       DAMZPP010000086        12163 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMZPP010000086 DAMZPP010000000
VERSION     DAMZPP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.086"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..200
                     /locus_tag="POH73_001789"
     CDS             <1..200
                     /locus_tag="POH73_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDI6887732.1"
                     /translation="SFEVAVPAFITIIMMPLSYSIATGIACGFIFYPITMLISKKHKE
                     VHPIMYVLMVLFILYFIFVHG"
     gene            complement(393..782)
                     /locus_tag="POH73_001790"
     CDS             complement(393..782)
                     /locus_tag="POH73_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887733.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            1139..1447
                     /gene="rpsJ"
                     /locus_tag="POH73_001791"
     CDS             1139..1447
                     /gene="rpsJ"
                     /locus_tag="POH73_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDI6887734.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            1475..2137
                     /gene="rplC"
                     /locus_tag="POH73_001792"
     CDS             1475..2137
                     /gene="rplC"
                     /locus_tag="POH73_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDI6887735.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            2164..2787
                     /gene="rplD"
                     /locus_tag="POH73_001793"
     CDS             2164..2787
                     /gene="rplD"
                     /locus_tag="POH73_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDI6887736.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKVQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            2787..3062
                     /gene="rplW"
                     /locus_tag="POH73_001794"
     CDS             2787..3062
                     /gene="rplW"
                     /locus_tag="POH73_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDI6887737.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            3095..3928
                     /gene="rplB"
                     /locus_tag="POH73_001795"
     CDS             3095..3928
                     /gene="rplB"
                     /locus_tag="POH73_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDI6887738.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            3995..4273
                     /gene="rpsS"
                     /locus_tag="POH73_001796"
     CDS             3995..4273
                     /gene="rpsS"
                     /locus_tag="POH73_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDI6887739.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            4302..4655
                     /gene="rplV"
                     /locus_tag="POH73_001797"
     CDS             4302..4655
                     /gene="rplV"
                     /locus_tag="POH73_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDI6887740.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            4679..5332
                     /gene="rpsC"
                     /locus_tag="POH73_001798"
     CDS             4679..5332
                     /gene="rpsC"
                     /locus_tag="POH73_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDI6887741.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            5335..5769
                     /gene="rplP"
                     /locus_tag="POH73_001799"
     CDS             5335..5769
                     /gene="rplP"
                     /locus_tag="POH73_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDI6887742.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            5759..5968
                     /gene="rpmC"
                     /locus_tag="POH73_001800"
     CDS             5759..5968
                     /gene="rpmC"
                     /locus_tag="POH73_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDI6887743.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            5992..6255
                     /gene="rpsQ"
                     /locus_tag="POH73_001801"
     CDS             5992..6255
                     /gene="rpsQ"
                     /locus_tag="POH73_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDI6887744.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            6287..6655
                     /gene="rplN"
                     /locus_tag="POH73_001802"
     CDS             6287..6655
                     /gene="rplN"
                     /locus_tag="POH73_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDI6887745.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            6691..7008
                     /gene="rplX"
                     /locus_tag="POH73_001803"
     CDS             6691..7008
                     /gene="rplX"
                     /locus_tag="POH73_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDI6887746.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            7035..7574
                     /gene="rplE"
                     /locus_tag="POH73_001804"
     CDS             7035..7574
                     /gene="rplE"
                     /locus_tag="POH73_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDI6887747.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFCK"
     gene            7597..7782
                     /locus_tag="POH73_001805"
     CDS             7597..7782
                     /locus_tag="POH73_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDI6887748.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            7814..8212
                     /gene="rpsH"
                     /locus_tag="POH73_001806"
     CDS             7814..8212
                     /gene="rpsH"
                     /locus_tag="POH73_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDI6887749.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            8237..8773
                     /gene="rplF"
                     /locus_tag="POH73_001807"
     CDS             8237..8773
                     /gene="rplF"
                     /locus_tag="POH73_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDI6887750.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            8804..9163
                     /gene="rplR"
                     /locus_tag="POH73_001808"
     CDS             8804..9163
                     /gene="rplR"
                     /locus_tag="POH73_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDI6887751.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            9184..9684
                     /gene="rpsE"
                     /locus_tag="POH73_001809"
     CDS             9184..9684
                     /gene="rpsE"
                     /locus_tag="POH73_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDI6887752.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            9701..9880
                     /gene="rpmD"
                     /locus_tag="POH73_001810"
     CDS             9701..9880
                     /gene="rpmD"
                     /locus_tag="POH73_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDI6887753.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            9897..10337
                     /gene="rplO"
                     /locus_tag="POH73_001811"
     CDS             9897..10337
                     /gene="rplO"
                     /locus_tag="POH73_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDI6887754.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            10337..11629
                     /gene="secY"
                     /locus_tag="POH73_001812"
     CDS             10337..11629
                     /gene="secY"
                     /locus_tag="POH73_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDI6887755.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            11646..>12163
                     /locus_tag="POH73_001813"
     CDS             11646..>12163
                     /locus_tag="POH73_001813"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDI6887756.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRL"
BASE COUNT         4397 a         1986 c         2439 g         3341 t
ORIGIN      
        1 aaagttttga agttgcagtg ccagcattca ttacaatcat tatgatgcct ttatcatatt
       61 caattgcaac tggtattgcg tgtggattta ttttctatcc aattacaatg cttatctcta
      121 aaaaacataa agaagtacat ccgataatgt atgtattaat ggtactattc attttgtact
      181 tcatatttgt tcatggttaa ctccagacac aaaaaacgac tgccaatgat tggtagtcgt
      241 tttttctttg aagtaaggat aaaataagaa gcgaaataac ataaagcaat attatgaatg
      301 tctttaaata tccgatataa atatacttct tttaaaattg ttgaacgtgt ttcttctata
      361 atatatatac ctaattaaaa aggttttaag aattattctc tatgtccatc tttgtattgt
      421 gtgtaaatga cacgagcatt tttattaggt atataggttg gcgctgataa tacaactagg
      481 acaaatagaa tcattaggaa aaataaaatc caatgaatca aaattcctaa gaaggggata
      541 atagctaaaa aactacctac tataccaaat attggaataa tcgctatagg tctaattgca
      601 ttttgtctat tcactaataa tataatagta agaattagat aaagtaatgc attaaagagt
      661 aaaggctgcc atccaaaact taaaataaac gttgcaccta aaaaaggaat gccatagaaa
      721 aactcacaaa gtattaaaaa gatacctata agtgctaaag ataattttgg tccatttttc
      781 atattcattc accaccgttc ttatgactaa ttatatagaa taattttcaa tgataataat
      841 aaaatgattc aaggtacgaa tttacaataa agaaggaaat aaaaaatatc tcgaaaatag
      901 ttgaactgac taagtatata aagtaaaata taaaagtatg tgttagacag atagaaaaat
      961 tatgtgaaaa gccttgattt atcgatgttt ttcgtgtaat ataactaacg ttggacttta
     1021 agaaaagcga tgaagcgaaa ggttactgac acacccggcc gctttgccat ggcgctgtgt
     1081 aagatagttt tcgtggagaa gtctatcact aaatgtagac gaataaggag ggaaaattat
     1141 ggcaaaacaa aaaatcagaa tcagattaaa agcttatgat caccgcgtga ttgatcaatc
     1201 agcagagaag attgtagaaa cagcgaaacg ttctggtgca gatgtttctg gaccaattcc
     1261 gttaccaact gagaaatcag tttacacaat catccgtgcc gtgcataagt ataaagattc
     1321 acgtgaacaa ttcgaacaac gtacacataa acgtttaatc gatattgtaa acccaacacc
     1381 aaaaacagtt gacgctttaa tgggcttaaa cttaccatct ggtgtagaca tcgaaatcaa
     1441 attataatag acaattttag gaggtggact ttcgatgacc aaaggaatct taggaagaaa
     1501 aattgggatg acacaagtat tcggagaaaa cggtgaatta atccctgtaa cagtagtaga
     1561 agctaaagaa aatgttgtat tacaaaagaa aactgtagaa gttgatggat acaacgcaat
     1621 ccaagttgga tttgaagaca aaaaagcata caaaaaagat gcaaaatcta acaaatatgc
     1681 taataaacca gctgaaggtc acgctaaaaa agctgacgca gcacctaagc gcttcattcg
     1741 tgaattccgc aatgtagacg tggatgctta cgaagtaggt caagaagtct cagtagatac
     1801 ttttgtagct ggcgacgtta ttgacgtaac aggcgtatca aaaggtaaag gtttccaagg
     1861 tgcaattaaa cgccacggac aatctcgtgg acctatgtca cacggttctc atttccacag
     1921 agcaccaggt tctgtaggta tggcttcaga tgcttctaga gtattcaaag gccaaaaaat
     1981 gccaggacgt atgggtggaa acactgtaac tgttcaaaac ttagaagtag ttcaagttga
     2041 cacagaaaac aaagttatct tagtaaaagg taatgtacct ggacctaaaa aaggtttagt
     2101 agaaatcaga acttcaatta aaaaaggtaa taaataataa gaagtgaaag gaggaaatgc
     2161 ataatggcta attatgatgt tttaaaatta gacggaacta aatcaggttc aatcgaatta
     2221 agcgatgcag tattcggtat tgagccaaat aatagcgttt tattcgaagc tattaattta
     2281 caacgtgctt cattacgtca aggtacgcat gctgttaaga atcgttcagc agtaagcggt
     2341 ggcggacgta aaccatggaa gcaaaaagga acaggtcgtg ctcgtcaagg tacaatccgt
     2401 gctccacaat ggcgtggcgg tggtatcgta ttcggaccaa ctccaagaag ttatgcatac
     2461 aaaatgccta agaaaatgcg tcgtttagct ttacgctcag cattatcttt caaagttcaa
     2521 gagaatggct taactgtagt tgacgcattc aacttcgaag ctccaaaaac taaagaattc
     2581 aaaaatgtat tatctacatt agaacaacct aaaaaagtat tagtagttac tgaaaacgaa
     2641 gatgtaaatg ttgaattatc agcacgcaac atccctggcg ttcaagtgac aactgctcaa
     2701 ggtttaaatg ttttagatat cactaatgct gacagcttag taattactga agctgctgct
     2761 aaaaaagttg aggaggtgct cggataatgg aagcaagaga tattcttaag cgccccgtaa
     2821 tcactgagaa atcttctgaa gcaatggctg aagacaaata cactttcgac gttgatactc
     2881 gtgttaacaa aacacaagtg aaaatggcag ttgaagaaat cttcaacgta aaagttgcaa
     2941 gtgttaatat catgaattac aaacctaaga aaaaacgtat gggccgttac caaggctata
     3001 caaacaaaag aagaaaagcg attgtaactc ttaaagaagg atcaatcgac ttatttaact
     3061 aataaaccaa ttaccattaa ggaggtaagc gacaatggct attaaaaagt ataagccaat
     3121 aacaaatggt cgtcgtaata tgacttcgtt agatttcgca gaaatcacga aaactacacc
     3181 tgaaaagtca ttattaaaac cgctaccgaa aaaagcggga cgtaacaacc aaggtaaatt
     3241 gactgtaaga caccatggtg gtggacacaa acgtcaatac cgtgttatcg atttcaaacg
     3301 taacaaagat ggtatcaatg caaaagttga ttctattcaa tatgatccaa accgctcagc
     3361 aaacatcgct ttagttgtat atgcagacgg tgaaaaacga tatatcattg ctcctaaagg
     3421 attagaagta ggtcaaatcg ttgaaagtgg tgctgaagct gacatcaaag ttggtaacgc
     3481 attaccatta caaaacattc cagttggtac agtagtacac aacatcgagc ttaaacctgg
     3541 taaaggtgga caaatcgctc gttcagctgg tgcaagtgct caagtacttg gtaaagaagg
     3601 taaatacgta ttaatcagat taagatctgg tgaagttcgt atgatcttat ctacttgccg
     3661 tgctacaatc ggtcaagttg gtaacctaca acacgaatta gttaacgttg gtaaagccgg
     3721 acgttcaaga tggaaaggta ttcgtccaac agttcgtggt tctgtaatga accctaacga
     3781 tcacccacac ggtggtggtg aaggtcgtgc tcctatcggt agaccatctc caatgtcacc
     3841 atggggtaaa cctacgcttg gtaagaaaac tcgtcgtggt aaaaaatcat cagacaaact
     3901 tatcgttcgt ggacgtaaga aaaaataata taatcaactt atttgggtgt gcggcttaaa
     3961 gctgcacgca cataataaga agggaggcgc ccaaatggct cgtagtatta aaaaaggacc
     4021 tttcgtcgat gagcatttaa tgaaaaaagt tgaagctcaa gaaggaagcg aaaagaaaca
     4081 agtaatcaaa acatggtcac gtcgttctac aattttccct aatttcatcg gacatacttt
     4141 tgcagtatac gacggacgta aacacgtacc tgtatatgta actgaagata tggtaggtca
     4201 taaattaggt gagtttgctc ctactcgtac attcaaagga cacgttgcag acgacaagaa
     4261 aacaagaaga taatatctat taagtagagg aggacatcct aatggaagca aaagcggttg
     4321 ctagaacaat aagaatcgca cctcgtaaag taagactagt tcttgactta atcagaggta
     4381 aaaatgctgc tgaagctatt gcaattttaa aattaacaaa caaagcttca tcaccagtaa
     4441 ttgaaaaagt attaatgtcc gctttagcta atgctgaaca taactatgac atgaacacag
     4501 atgaattagt agttaaagaa gcatatgcta acgaaggacc aacattaaaa cgtttccgtc
     4561 cacgtgcgca aggtcgtgca agtgcgatta acaaacgtac aagccacatt acaatcgtcg
     4621 taagtgacgg taaagaagaa gctaaagaag cttaattaac ttttaaggag ggaatactgt
     4681 gggtcaaaaa attaatccaa tcggacttcg tgttggtatt atccgtgatt gggaagctaa
     4741 atggtatgct gaaaaagact tcgcttcact tttacacgaa gatttaaaaa tccgtaaatt
     4801 tattgataat gaattaaaag aagcatcagt ttctcacgta gagattgaac gtgctgcaaa
     4861 ccgtatcaac attgcaattc atactggtaa acctggtatg gtaattggta aaggcggttc
     4921 agaaatcgaa aaattacgca acaaattaaa tgcgttaact gataaaaaag tacacatcaa
     4981 cgtaattgaa atcaaaaaag ttgatcttga cgctcgttta gtagctgaaa acatcgcacg
     5041 tcaattagaa aaccgtgctt cattccgtcg tgtacaaaaa caagcaatca ctagagctat
     5101 gaaacttggt gcaaaaggta tcaaaactca agtatctggt cgtttaggcg gagctgacat
     5161 cgctcgtgct gaacaatatt cagaaggaac tgttccactt catacgttac gtgctgacat
     5221 cgattatgca cacgctgaag ctgacactac ttacggtaaa ttaggcgtta aagtatggat
     5281 ctatcgtgga gaagttcttc ctactaagaa cactagtgga ggaggaaaat aataatgtta
     5341 ctaccaaaac gtgtaaaata tcgtcgtcaa catcgtccta aaacaactgg tcgttctaaa
     5401 ggcggtaact acgtaacatt tggtgagttt ggtttacaag ctacaacaac gtcttggatc
     5461 acatctcgtc aaatcgaatc tgctcgtata gcaatgacac gttacatgaa acgtggcggg
     5521 aaagtttgga ttaaaatctt cccacataca ccatatacta aaaaaccttt agaagtacgt
     5581 atgggtgctg gtaaaggtgc ggttgaaggc tggatcgcag ttgttaaacc aggtagaatt
     5641 ttattcgaag ttgctggcgt ttctgaagaa gttgcgcgtg aagcactacg tttagcaagt
     5701 cacaaacttc cagtaaaaac taagtttgta aaacgtgagg aattgggtgg tgaaacaaat
     5761 gaaagctaag gaaattagag acttaaccac ttcagaaatc gaagaacaaa tcaaatcttc
     5821 aaaagaagag ctttttaacc tacgctttca gttagctaca ggtcaattag aagaaactgc
     5881 acgtattcgt acagtaagaa aaacgattgc acgtctaaaa actgttgctc gtgaaagaga
     5941 aattgaacaa agtaaggcta atcaataatt aagtaagagg aggttacaaa agtgagcgaa
     6001 agaaacgatc gtaaagttta tgtaggtaaa gttgtttcag acaaaatgga caagactatt
     6061 acagtacttg ttgaaactta caaaacacac aaattatacg gtaaacgagt gaaatactct
     6121 aaaaaataca aaactcatga tgaaaacaat tcagctaaat taggagacat tgttaaaatt
     6181 caagaaactc gtcctttatc agcaacaaaa cgttttcgtt tagtagagat tgttgaagag
     6241 tcagtaatta tttaatacaa gtttagagat aagggaggtt taactaatga tccaacaaga
     6301 aacacgcttg aaagtagcag acaactctgg tgctcgtgaa gttcttacaa tcaaagtatt
     6361 aggtggatct ggtcgtaaaa cagcaaacat cggcgatgtt atcgtatgta ctgttaaaaa
     6421 tgcaacacca ggtggcgttg ttaaaaaagg tgacgttgtc aaagctgtaa tcgtacgtac
     6481 taagtcaggt gttcgtcgta atgacggttc atatatcaaa tttgatgaaa atgcatgtgt
     6541 tatcatccgt gatgacaaag gcccacgtgg tactcgtatc ttcggacctg ttgctcgtga
     6601 attacgtgaa ggtaacttca tgaaaatcgt atcattagca ccagaagtac tttaattaat
     6661 aacaaccaat acaaataagg aggtgcccac atgcatatca aaaaaggtga caacgttaaa
     6721 gttatcgcag gtaaagacaa aggtaaagaa ggtaaagtaa tcgctactct acctaaaaaa
     6781 gaccgtgtcg ttgtggaagg tgttaacatt atgaaaaaac accaaaaacc aactcaatta
     6841 aatcctgaag gtggaatctt agaaacagag gcagcaatcc atgtttctaa tgtacaatta
     6901 ttggacccta aaacaaacga accaactcgt gtaggttaca aatttgttga tggtaaaaaa
     6961 gttcgtatcg ctaaaaaatc tggcgaagaa attaaatcta ataattaata acaagttgaa
     7021 aggaggatcc cactttgaac cgtttaaaag aaaagtttaa cactgaagtt actgaaaact
     7081 taatgaaaaa attcaattat agttcagtaa tggaagtacc aaaaatagat aaaatcgttg
     7141 tgaacatggg tgtaggtgac gcagtacaaa attctaaagt attagacaat gctgttgaag
     7201 aattagaatt gatcactggt caaaaaccat tagtaactaa agctaaaaaa tcaatcgcga
     7261 ctttccgttt acgtgaaggt atgccaatcg gtgcgaaagt aacacttcgc ggtgaaagaa
     7321 tgtatgaatt cttagacaaa ttaatttcag tatcattacc acgtgtacgt gacttccaag
     7381 gtgtttctaa aaaagcattt gacggacgcg gtaactacac tttaggtgtt aaagaacaat
     7441 taattttccc agaaatcgac tatgataaag taagtaaagt tagaggaatg gatattgtta
     7501 tcgtaacgac tgctaacact gatgaagaag ctcgtgaatt gttagctaac ttcggtatgc
     7561 cattctgtaa ataattattt aaaggaggct aattaagtgg ctaaaacttc aatggttgct
     7621 aagcaacaaa aaaaacaaaa atatgcagtt cgtgaataca ctcgttgtga acgttgtggc
     7681 cgtccacatt ctgtatatcg taaatttaaa ttatgccgta tttgtttccg tgaattagct
     7741 tacaaaggcc aaatccctgg cgttcgtaaa gctagctggt aataaaaaag agtctgaaag
     7801 gaggcaacaa tcaatgacaa tgacagatcc aatcgcagat atgcttactc gtgtaagaaa
     7861 cgcaaacatg gtgcgtcacg agaagttaga attacctgca tcaaatatta aaaaagaaat
     7921 tgctgaaatc ttaaagagtg aaggtttcat taaaaatgtt gaatacgtag aagatgataa
     7981 acaaggtgta cttcgtttat tcttaaaata tggtcaaaac gatgagcgtg ttatcacagg
     8041 attaaaacgt atttcaaaac caggtttacg tgtttatgca aaagctagcg aaatgcctaa
     8101 agtattaaat ggtttaggta ttgcattagt atcaacttct gaaggtgtaa tcactgacaa
     8161 agaagcaaga aaacgtaatg ttggcggaga aattatcgca tacgtttggt aataaaaaat
     8221 aaggaggtgc cataacatga gtcgtgttgg taagaaaatt attgacatcc ctagtgacgt
     8281 aacagtaact tttgatggaa atcatgtaac tgttaaaggt cctaaaggtg aattatcaag
     8341 aactttaaat gaaagaatga cattcaaaca agaagaaaac acaattgaag ttgtaagacc
     8401 atctgattct aaagaagata gaacaaacca tggtacaact cgtgctttat taaataatat
     8461 ggtacaaggt gtttctcaag gatacgtaaa agtacttgaa cttgttggtg taggttaccg
     8521 tgctcaaatg caaggtaaag acttaatcct taacgttggt tattctcacc cagtagaaat
     8581 taaagctgaa gaaaacatta ctttctcagt tgagaaaaac acagtcgtta aagttgaagg
     8641 tatttcaaaa gaacaagttg gagcattagc atctaacatc cgttcagtaa gacctccaga
     8701 gccttacaaa ggtaaaggta ttcgttacca aggtgaatac gttcgccgta aagaaggtaa
     8761 aactggtaaa taatagataa actctaaaga aaggagtatt gaaatgatca gtaaaattga
     8821 taaaaataaa gtgcgtttaa aaagacatgc tcgtgttcgt actaacttat caggtacagc
     8881 tgaaaagcca cgtttaaacg tatatcgttc aaacaagcat atctacgctc aaattattga
     8941 tgataataaa ggcgtaacat tagctcaagc ttcttcaaaa gacagcgaca ttgctactac
     9001 agcaactaaa gttgaattag caactaaagt cggtgaagca attgctaaaa aagctgctga
     9061 caaaggcatt aaagaaatcg tatttgaccg tggaggatat ttatatcacg gacgtgttaa
     9121 agcattagct gaagcagcaa gagaaagcgg attagaattt taatttaaag gagggacaaa
     9181 tacatggctc gtagagaaga agagacgaaa gaatttgaag aacgcgttgt tacaatcaac
     9241 cgtgtagcaa aagttgtaaa aggtggtcgt cgtttccgtt tcactgcatt agttgtagtt
     9301 ggagacaaaa atggtcgtgt aggtttcggt actggtaaag ctcaagaggt accagaagca
     9361 atcaaaaaag ctgttgaagc agctaaaaaa gatttagtag ttgttccacg tgttgaaggt
     9421 acaactccac acacaattac tggccgttac ggttcaggaa gcgtatttat gaaaccggct
     9481 gcacctggta caggagttat cgctggtggt cctgttcgtg ccgtacttga attagcaggt
     9541 atcactgata tcttaagtaa atcattagga tcaaacacac caatcaacat ggttcgtgct
     9601 acaatcgatg gtttacaaaa ccttaaaaat gctgaagatg ttgcgaaatt acgtggcaaa
     9661 acagtagaag aattatacaa ttaaggaggg aaaactagtt atggctaaat tacaaattac
     9721 cctcactcgt agtgttattg gtcgtcctga aacacaacgt aaaactgttg aagctttagg
     9781 tcttaaaaag actaacagtt cagtagttgt tgaagataac cctgctattc gtgggcaaat
     9841 caacaaagtt aagcacttag taacagtaga agaaaaataa tcaaggaggt gccgaaatga
     9901 aattacatga gttaaaaccg gcagaaggtt cacgtaaaga acgcaatcgt gttggacgtg
     9961 gtgttgcgac aggtaacggt aaaacaagtg gtcgcggaca caaaggtcaa aaagctcgtt
    10021 caggcggtgg tgtaagacca ggattcgaag gtggtcaatt accattattc cgtcgtttac
    10081 caaaacgtgg ttttactaac ataaatcgta aagaatatgc tattgttaac ttagaccaac
    10141 ttaataaatt tgaagatggt actgaagtaa ctccagcttt attagtagaa tctggtgttg
    10201 ttaagaatga aaaatctggt atcaaaatac taggtaatgg ttcacttgat aagaaattga
    10261 cagtgaaagc tcataaattc tcagcttcag cagcagaagc tattgatgct aaaggtggag
    10321 cacacgaggt gatctaatga ttcaaaccct tgtgaacttc tttagaacaa aagaggttcg
    10381 taacaagatt ttcttcacac tagcaatgtt agtaattttt aaaataggga cttatatacc
    10441 agctccagga gtaaatcctg cagcttttga taatccccaa ggttctcaag gtgccactga
    10501 gttattaaat acttttggtg gcggagcctt gaaacgattt tctatttttg caatgggtat
    10561 tgtaccctac atcactgcat caatcgtaat gcaattatta caaatggata ttgtccctaa
    10621 attctcagaa tgggcaaaac aaggtgaagt aggtagaaga aagttaaata acgttactcg
    10681 ttatttagca atttctttag catttatcca atctataggt atggcattcc aatttaataa
    10741 ttatctcaaa ggtgcgctga ttatcaatca gtcaattatg agttatttat taatagcact
    10801 agttttgaca gcaggaactg ctttcttaat atggcttggt gatcaaatca ctcagttcgg
    10861 tgttggtaat ggtatttcta ttatcatatt tgcgggtatt ttatcaacgt tgccagccag
    10921 tctaattcaa tttggacaaa ctgcgttcgt tgggcaagaa gatacatcat tagcatggtt
    10981 gaaagtatta ggacttttag tgtcactgat actattaact gtaggtgcta tttatgtact
    11041 tgaagccgtt cgtaaaattc cgattcaata tgctaagaaa caaactgctc aaagattagg
    11101 ttcacaggca acttatctac ctttaaaagt gaactcagcc ggtgttattc ctgtaatctt
    11161 tgcaatggcg ttcttcttac tgcctagaac attaacgttg ttctatccag ataaagaatg
    11221 ggcacaaaac attgcgaatg ctgcaaatcc ttcaagtaat gttggtatgg ttgtatatat
    11281 tgtactaatt atactattca catacttcta tgcatttgta caagttaatc cggaaaaaat
    11341 ggctgataac cttaagaaac aaggtagcta tgttccaggt attagacctg gtgaacaaac
    11401 caaaaaatat attactaaag ttttatatcg tttaactttt gtaggttcta tcttcttagc
    11461 cgttatttca attctaccga tattagcaac taagtttatg ggattaccac aatcaattca
    11521 gattggtggt acaagtttac ttatcgttat tggtgtagcg attgaaacaa tgaaatcttt
    11581 agaagcacaa gtatctcaaa aagaatataa aggctttggt ggtagataat tttaggaggg
    11641 cacttatgaa tatcattttg atgggtttac ctggcgcagg taaaggaact caagcaagtg
    11701 aaattgtcaa gaaattccca ataccccaca tttcaactgg tgacatgttc agaaaagcta
    11761 taaaagaaga aactgaatta ggtaaagaag ctaagtctta tatggaccgt ggcgaattag
    11821 ttcctgatga agtgactgta ggtatcgtta aggaaagaat ttctgaagac gatgcaaaaa
    11881 aaggcttttt attagatggc ttcccaagaa caatcgagca agctgaggca ttaaataata
    11941 ttatgtctga gcttgacaga aacattgatg ctgtcatcaa tatcgaagtt ccggaagaag
    12001 aattaatgaa ccgtcttaca ggtcgtcgaa tctgtgagtc atgtggtaca acgtatcatc
    12061 ttgtatttaa tcctccgaag gtcgaaggta tttgtgatat cgatggtggt aaattgtatc
    12121 aacgagaaga tgataatcct gaaacggtag ctaatcgttt gag
//
LOCUS       DAMZPP010000087        12065 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMZPP010000087 DAMZPP010000000
VERSION     DAMZPP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12065
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.087"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..478
                     /locus_tag="POH73_001814"
     CDS             <1..478
                     /locus_tag="POH73_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDI6887757.1"
                     /translation="FVCVIINNKYAISVPDSLQYAAEKLSDRALGYGLHGEQVDGNDP
                     LAMYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEEREALKKADCNEK
                     FKKELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            478..1461
                     /locus_tag="POH73_001815"
     CDS             478..1461
                     /locus_tag="POH73_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDI6887758.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGKKGGVFGTTQGL
                     QQQYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            1474..2748
                     /locus_tag="POH73_001816"
     CDS             1474..2748
                     /locus_tag="POH73_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDI6887759.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASSTVEQTSTSKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDLMSVI
                     ENGGTTAQSDKQVQTQSTSVDRSSNQSSEDDSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNNFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTTEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIVKKPVVINDMIAIRSMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            3098..3535
                     /locus_tag="POH73_001817"
     CDS             3098..3535
                     /locus_tag="POH73_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDI6887760.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGNITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            3549..4529
                     /locus_tag="POH73_001818"
     CDS             3549..4529
                     /locus_tag="POH73_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDI6887761.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEALLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(4656..4835)
                     /gene="prli42"
                     /locus_tag="POH73_001819"
     CDS             complement(4656..4835)
                     /gene="prli42"
                     /locus_tag="POH73_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HDI6887762.1"
                     /translation="MLVISSQVKNFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
     gene            5098..6231
                     /locus_tag="POH73_001820"
     CDS             5098..6231
                     /locus_tag="POH73_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDI6887763.1"
                     /translation="MINEQRLLNTFLELVQIDSETGNESTIQPILKEKFISLGLDVKE
                     DEAAKHPKLGANNLVCTMKSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNETVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARQSK"
     gene            6299..7705
                     /gene="gndA"
                     /locus_tag="POH73_001821"
     CDS             6299..7705
                     /gene="gndA"
                     /locus_tag="POH73_001821"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDI6887764.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            8021..9234
                     /locus_tag="POH73_001822"
                     /pseudo
     CDS             8021..9234
                     /locus_tag="POH73_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9249..10148
                     /locus_tag="POH73_001823"
     CDS             9249..10148
                     /locus_tag="POH73_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HDI6887765.1"
                     /translation="MKNSQNIVCIGAANIDKQLYLKSELVNKSSNPVSSISVSGGVAR
                     NLAYNIGKLSNKKTILLTLLGNDKEADYILNEANKFIDLKHIKIIENMNTGMFTSVID
                     YNGEMICGFADMDIYNLINNKYLNEKIEVIKNADCVVVDSNIPKESIQYLIQLCGKSN
                     IPIGIIPASIKKVRNILKNLAKIDYLIFNKKEAEAFWNVSLNTKSDYKKIKKISKTSE
                     IKNIVITNGNDDIFYTNGELSIFKNVQQSSNIINDTGAGDVFSASVFHYWLENYEVDN
                     YLEMALFNACSTVSTIYNTSMIN"
     gene            complement(10220..10381)
                     /locus_tag="POH73_001824"
                     /pseudo
     CDS             complement(10220..10381)
                     /locus_tag="POH73_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            10625..10996
                     /locus_tag="POH73_001825"
     CDS             10625..10996
                     /locus_tag="POH73_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6887766.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTEILEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKYSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQNGQGNYFAVSD"
     gene            10935..11189
                     /locus_tag="POH73_001826"
     CDS             10935..11189
                     /locus_tag="POH73_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887767.1"
                     /translation="MVNVYLISKMAKVIILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYKGWKRFPVQRLTGGD"
     gene            11190..12065
                     /locus_tag="POH73_001827"
                     /pseudo
     CDS             11190..12065
                     /locus_tag="POH73_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
BASE COUNT         4356 a         1596 c         2223 g         3890 t
ORIGIN      
        1 ttttgtatgt gtcattataa ataataaata tgcaatttca gtacctgatt cattacaata
       61 cgcagctgaa aaattatcgg atagagcact tggttacggt ttacatggtg aacaagtaga
      121 tggtaatgat ccattagcaa tgtataaagc gatgaaagaa gcaagagatc gagctatttc
      181 tggtcaaggt tcaactttga ttgaagcagt aacaagtcgt atgacagctc attcatcaga
      241 tgatgacgac caatatcgta cgaaagaaga gcgtgaagcg cttaaaaaag cagactgcaa
      301 tgaaaagttc aaaaaagagt tgctttcagc tggcattatc gatgatgctt ggttggcaga
      361 aatagaagcg gagcataaag acatcatcaa taaagcgaca aaggctgctg aagacgcgcc
      421 atatccaagt gtcgaagaag cctatgcatt tgtgtacgaa gaagggagtc ttaactaatg
      481 gctaaattat cttatttaga ggcgattcgc caggcacaag acttagcttt acaacaaaat
      541 aaagatgtat ttattcttgg tgaagatgtt ggtaaaaaag gtggcgtatt tggtactact
      601 caaggactgc aacaacaata tggtgaagat agagttatcg atacgccatt agctgagtca
      661 aatattgttg gaactgccat tggtgcagct atggtaggta aaagaccaat tgcagaaatt
      721 caatttgcag attttatttt acctgcaaca aatcaaatta ttagtgaagc tgcgaaaatg
      781 cgttatcgtt caaataatga ttggcaatgc ccattaacga ttcgtgcacc atttggtggc
      841 ggtgttcacg gaggactata tcattcacaa agtattgaaa gtatatttgc ttcatcacca
      901 ggtttaacaa ttgttatacc ttcaacaccg tatgatgcta aaggtctatt attatcttca
      961 attgaatcaa atgatcctgt tttatacttt gaacataaaa aggcttatag attcttaaag
     1021 gaagaagtgc cagaagaata ttacactgta cctttaggaa aagcagatgt taaacgtgaa
     1081 ggtgaagatt taacagtatt ttgttatgga ttaatggtca attattgttt acaagccgct
     1141 gatattttag cagcagacgg tatcaatgtt gaagttgtag atttacgaac tgtttatcca
     1201 ttagataaag aaacaattat tgatcgtgct aaacatacag gtaaagtttt acttgttact
     1261 gaagataatt tagaaggtag cattatgtca gaagtgtcag cgattattgc agagcattgc
     1321 ttgttcgatt tagatgcacc aatcatgcgt ttagctgctc cagatgtacc atctatgcca
     1381 ttttcacctg tattagaaaa tgaaattatg atgaatccag aaaaaatctt aaataaaatg
     1441 cgtgaattag cagaattcta gggagggaaa gtcatggaaa taacaatgcc taagttaggt
     1501 gagagtgttc atgaaggtac cattgaacaa tggttagttt ctgttggtga tcatattgat
     1561 gaatatgaac cattatgtga agttattaca gataaagtga cggctgaagt cccttccacg
     1621 atatcaggaa caattacaga aattttagtt gaagcggggc agacggtagc tattgataca
     1681 attatctgta aaattgaaac tgctgatgaa aagacaaatg aaacaactga agagatacaa
     1741 gcaaaagtgg atgagcatac tcagaaatct actaaaaaag ctagttcaac agtggaacag
     1801 acatctactt ctaaacaaaa tcaaccacga aataatggtc gcttttcacc tgttgtattt
     1861 aaactcgctt cagagcatga cattgattta tcacaagttg taggtagtgg atttgaaggt
     1921 cgtgtaacta agaaggattt aatgtcagtt attgaaaatg gtggtaccac agctcaatct
     1981 gacaaacaag ttcaaacaca atcaacttca gtagatagat caagtaacca atcatctgaa
     2041 gacgatagtg aaaatagcac aataccagta aatggtgtgc gtaaagcaat tgcacaaaac
     2101 atggttaata gtgtaacgga gattccacat gcatggatga tgattgaggt tgatgctaca
     2161 aaccttgtga atacgagaaa ccattataaa aacaacttta aaaataaaga aggatataat
     2221 ttaacgttct ttgctttctt tgtaaaagct gtagcagatg ctttaaaagc atatccatta
     2281 ttaaatagta gctggcaagg aaatgaaatt gtcttacata aagacattaa tatttcaatt
     2341 gctgttgctg atgaaaataa attatacgta cctgtgatta agcatgcaga cgaaaagtca
     2401 atcaaaggta tagctagaga aattaatact ttagcaacaa aagcgcgtaa taagcaattg
     2461 acaactgaag atatgcaagg tggaacattt acagttaata atactggtac atttggttca
     2521 gtatcatcaa tgggtattat aaatcaccct caagcagcga ttttacaagt agaatcaatc
     2581 gttaaaaagc cagtagtaat taatgatatg attgcaattc gaagtatggt taatttatgt
     2641 atttcaattg atcatcgtat tttagatggt ttacaaacag gtaaatttat gaatcatatt
     2701 aaacagcgta tcgaacagta tactttagaa aatacaaata tatattagtg ataacataga
     2761 tgcatctatc gacaacttgt tttatcttgt tcttgtcgat ggatgtattt ttttggcaat
     2821 aaaatatttg caatatattc aaaaaaagat aaagaacaat aatcaacatg gttgaatgca
     2881 tttttgcagt aaagtcaaaa taagacatcg tacttgaaac atattaaaga aaacaggtga
     2941 acaaattagt ttccatgatt ttaagcacaa taatgtttgg tatattgtta aaattgtgtc
     3001 taaatatagg tgtgattcag attagtttat tgaacaatat gttattaatt agtagaatga
     3061 ggatagttta aatataaagg gataggtgat tgaacttatg gacatgaatt tcgatttata
     3121 catgaacggt gttgtagaac aagcaaggaa tgaaattgaa tctgcgggat atgagcaatt
     3181 aactactgca gaagatgttg acaaagttct taaacaagat ggtacaacac tagttatgat
     3241 caattctgta tgtggttgtg caggtggtat cgcaagacca gcagcatcac atgctttaca
     3301 ttatgacgta ttacctgatc gtctagtgac agtatttgct ggacaagata aagaagcgac
     3361 acaaagagcg cgtgaatact tcgaaggtta tgcgccttca agtccgtcat ttgcattagt
     3421 aaaagatgga aatattacag aaatgattga aagacatcaa atcgaaggtc atgatgtgat
     3481 gaacgtaatt aatcaattac aaacattatt caataaatat tgtgaagaaa gataagaggc
     3541 gctaacccat gttaaagtta aatccttaca agattggatt tagaacaata aaaacagcag
     3601 tgggtatgac tttaggtgta attattagta agctgttagg tttagataat tatgcttcaa
     3661 gtgccatatt agtcgtatta tgtattaaac atacaaaagt acattcgcta caagcgatta
     3721 tttcaagatt agtatcatgt tttttagtat tgtttttagg ttcagcaata tttagtttat
     3781 taggccagag tccaattgta ctcggtatta tcgtattgtt atttatacca ttaactgtcg
     3841 tattaaaagt acaagaaggt gtcattacga gttgcgttat attacttcat gtttttaatg
     3901 caaaatcaat tgatgcacat ttaattgtta atgaaacatt attactgtta attggactaa
     3961 gcattgcatt tacaatgaat ttaatgatgc caagtttaga caaacaacta gacgaataca
     4021 aatgtaaaat tgagcaacaa attgctgata tttttagtaa atatagttat atttgtgaaa
     4081 aatatgaaga taccattgcg attgaatttg aagcgttact tttaaatatt aaaaaggcga
     4141 agtctatcgc gttccgagat gttaaaaatc attttgttag aaacgaaaat tcatactatc
     4201 attattttga tatgcgagaa gagcaagtgg aattgttaat gagaatgaaa ccgctcatcg
     4261 aaagtatctg tcataaagat ccacttttaa atgatgttgc gtcactgctg aaagaaattg
     4321 gtctaaatgt gaatagtaat gactatacag caatgcgttt acacaatttg tatgagacgc
     4381 gtttgaaatt ggatgaactg ccattgccaa caacacacaa agcgcttaac tcgagagcaa
     4441 gtattattca aatattaaat gaattagaag aatatttaca aatcaaatcg caatttggtt
     4501 cattgaaaat gcatagtgaa gtagcttaaa aaataatcca tccgtatgac ccatgtttga
     4561 aagtaatcgt tgcaatacga taacttatta atacatgcgt ttataacgga tggattttta
     4621 attgtatgat tttgtatagg tagttgagat atggtttata aaattggaat gatactcgtt
     4681 aataacaatg ctaatacaac tgatacaagc ataacgataa tgataattct taaaacttta
     4741 ttttgcattt tgtgtgcctc caaatgaata acctttgtat taataattac gacataagat
     4801 ataaaatttt taacttgaga cgatataact agcaatcatc ataaaggaat attataaaaa
     4861 acatgagtaa gtttgcttat gtcattttcc tcattatacg atatctttga aaaatcaatc
     4921 aatatatttt tgacaaagct tagtttgagt tacaataaat atatatatta aattgaaaat
     4981 aatatgacac aatttacaag cctgataaat ctgtgtaacg tcaattactg taacatttta
     5041 attcatcaat ttttaccgtt tggcgtactt gaaaattttg ataaaagggg ttccattatg
     5101 attaatgagc aaagattact taatacgttt ttagaacttg tacaaatcga ttctgagaca
     5161 gggaatgaat caacaatcca acctatttta aaagaaaaat tcatatcatt agggttagat
     5221 gttaaagaag atgaagcggc taaacatcct aaattaggtg ccaataatct agtgtgtaca
     5281 atgaaaagta ctatcgaaga aggcgaagta cctaaattat atctaacgag ccatatggat
     5341 actgttgtac ccgcaattaa tgtaaagcca attgtaaaag atgacggcta catatactct
     5401 gacggtacga ctattctagg tgcagatgat aaagcaggat tagcagctat gcttgaagtt
     5461 ttgcaagtga taaaggaaca acaaatccca catggacaaa ttcaatttgt gattactgtt
     5521 ggggaagaat caggtttaat tggtgctaaa gaattaaatt cagagttgtt ggacgcggac
     5581 tttggttatg ctattgatgc aagtgctgat gtcggtacta cggttgtagg tgcaccgacg
     5641 caaatgttaa tttcagctaa aatttttggc aaaacggctc atgcaagtac gccaaaagag
     5701 ggtgttagtg ctattaatat tgcggcgaaa gcaattagtc gaatgaaatt aggacaagtt
     5761 gatgaaatca ctactgccaa tattggtaag tttcatggcg gttcagctac gaatattgtt
     5821 gcagatgaag tcattttaga agcagaggca cgttctcacg atccagaaag aataaaaaca
     5881 caagtcaaac atatgactga tgtgtttgaa actactgcat cagaattagg tgggaaagca
     5941 gtagttacag tggagcaaag ttatccagga tttaaaatta atgataatga aacggttgtt
     6001 aaaattgcac aagaaagtgc tcgtaattta ggattatctg caaatacaat tatttctggt
     6061 ggtggttcag acggcagtat tattaataca tttggtattc cttcagtcat tttaggagtg
     6121 ggttatgaaa aaatccatac aacaaatgag agaatgccga ttaaatcatt gaacttgctt
     6181 gcaagtcaag tgttagaaat aatcaaaata gttgcgcgac aatcgaaatg atttagtgta
     6241 gtgtatgtct attttgtgat acaatactaa tgaaaaatca acaagtgagg taagtaatat
     6301 gacacaacaa attggagtta ttggtttagc cgttatgggt aaaaacctag cttggaatat
     6361 tgaatcacgc ggatatagtg tatctgtatt taaccgctca agtgaaaaaa ctgatttaat
     6421 ggttgaagaa tcaaaaggga aaaatattca tccaacgtat tcattggaag aatttgttaa
     6481 ttctttagaa aaaccacgta aaattttatt aatggttcaa gcaggtaaag ctacggatgc
     6541 aacgattgat agtttgttac ctttattaga tgatggagat attttaattg atggtggtaa
     6601 tacaaactat caagatacta tcagacgtaa taaagcatta gcacaaagtg caatcaactt
     6661 tattggtatg ggcgtttctg gtggtgaaat tggagcgtta actggtccgt cattaatgcc
     6721 gggtggacaa gaagaagctt ataacaaagt tgcagatatt cttgatgcaa ttgctgcaaa
     6781 ggcaaaagac ggtgcttcat gtgttactta tattggacca aatggtgctg gacactatgt
     6841 gaaaatggta cacaatggta tcgaatatgc tgacatgcaa ttaattgcag aaagttatgc
     6901 tatgatgaaa gaattattag gcatgtctca tgaagacatt gctcaaacgt tcaaagactg
     6961 gaatgctggt gaattagaaa gttatttaat tgaaatcact ggtgatattt tcatgaaatt
     7021 agatgaaaat aaagaagcat tagttgaaaa aattcttgat actgcaggtc aaaaaggtac
     7081 aggaaaatgg acttcaatta acgcattaga attaggtatt ccattaacaa tcataactga
     7141 atctgtattt gcacgtttta tctcttcaat caaagaagag cgcgtaaatg cttctaaaga
     7201 attaaatggt ccaaaagcat catttgatgg tgacaagaaa gactttttag aaaaaattcg
     7261 taaagcatta tatatgagta aaatttgttc ctatgcacaa ggtttcgcgc aaatgcgtaa
     7321 agcaagtgaa gataatgaat ggaatttaaa acttggtgat ttagctatga tttggagaga
     7381 aggttgcatt atccgtgcac aattcttaca aaagatcaaa gatgcttacg ataataatcc
     7441 tggattacaa aacttattgc tagatcctta ctttaaaaac atcgttactg aatatcaaga
     7501 tgcgttacgt gatgttgtcg caactggtgt tcaaaatggt gtaccaacac ctggtttctc
     7561 atcaagtatt aattactatg atagttaccg tgcagctgat ttacctgcaa acttaattca
     7621 agcacaacgt gactacttcg gtgctcacac gtacgaaaga aaagataaag aaggcgtatt
     7681 ccatacacaa tggattgaag aataatattg actattgagc ctgagacctt atgaatttag
     7741 gtaatgttaa agagacaatt tctattattt atttgataga aattgtctct ttccttatgt
     7801 atttatcgaa atttactacg catgatatat tcaatgattt tctttctctt gtcaaattaa
     7861 aatttttaac ttttataaat taacgatatc tatcctcata gaatttgtcc gtttaagtgt
     7921 agacgtttta cttacatact attaagatag cattagttaa taattttaat atttagataa
     7981 atagattacg ttttattatt tgatgaagaa gagggataga atgtcatatg cttctgtatt
     8041 gtttaataag aactttactt tttatttaat agggatagct ttttctaaca taggcagtgc
     8101 ttttactacg tttgttttcc gttaatgatt ttaaagctta ctggctctgc atttcaagtt
     8161 ggcatagttt ctgcactaag ttttatacct tatgcaattt taggactgcc agcaggtgct
     8221 ttaattgaca gattgaataa gaagaccata atgaaatgtg ctgatataat taggctcatt
     8281 tcatatttaa gtataccagt attaagtttt tataatatgt taagcatctt tcaaatttat
     8341 gtagttgcaa tcatttcagg tattggatta gtatttcaca gtatttctga ggtttcgata
     8401 atcccaagta ttgtaaaaga agaagatttg gcgagcgcaa acagttatat atacgctact
     8461 caaaatgttt cggaattttt gggccctata attggaggtt tactttatac atatatggga
     8521 tattcaatat tgatttttat agattctatg acttttttac tctcattttt cagtttgata
     8581 ttaattaaaa ttgaaacaaa gagtatattc aatcaagaaa agttgagtgg aaaaaatttt
     8641 ttgagcgatg ttaaagttgg atttgattat ctattatcta attcaactat tagagtaatg
     8701 gctgttgttg taagtttatc caatctaatt atctcacctt attatattta tatagtaatg
     8761 tttgtaaagg aagacatgaa tcaatcatct caagcattgg ggttagtttt aggtatctca
     8821 tcattaggtg cgctaatcgg ttcattatct gcaagttttc tcttgaaatt atttaatttc
     8881 ggaaaactaa ttgtaattat attattttta gatacaattt ttagattaat gttaccattt
     8941 agtacttata tttttatact catcccactt ttagggttga catatatgac acaatccatt
     9001 ttaaatatag cgataatcac actgaggcaa aagaaatgtt cagaacatat gttaggacgt
     9061 gttaatagtg tttttaaaac aatggttttt gcattcagag ctataggcct ttttttaggc
     9121 ggcatattac ttgaaaataa aggtggattt tatgcactta cgatcagcgc tgtgttattt
     9181 ataccattag tgttatatat actgaaaaat agattttata aagtagaaga atagtaagga
     9241 gaaaattaat gaaaaacagc caaaatattg tttgtattgg tgcagcaaat atagataaac
     9301 aattatattt aaaatctgaa ttggttaata aaagttcaaa ccctgtatct tcgataagcg
     9361 tatctggagg tgtagctagg aatttagcgt ataatattgg caaattaagt aataaaaaaa
     9421 ctattctatt aactttatta ggtaatgata aagaagctga ttatatttta aatgaagcta
     9481 ataagtttat cgatttaaaa catataaaaa ttatagaaaa tatgaacaca ggaatgttca
     9541 cttctgtaat agattataat ggggaaatga tatgtggttt tgcagatatg gatatatata
     9601 acttaataaa taacaaatat ttaaatgaaa aaatagaagt aataaaaaat gcagattgtg
     9661 tggttgtaga ctctaatatt cccaaggaaa gtatacaata tttaattcag ttgtgtggaa
     9721 agagtaatat tccaataggt ataatcccag catctataaa gaaggtgaga aacatactaa
     9781 aaaacttagc aaagatagac tatttaatat ttaataaaaa agaagctgaa gctttttgga
     9841 acgtttcttt aaatactaaa agtgattata aaaaaataaa aaaaattagt aaaacttcag
     9901 aaataaaaaa tatagttatc actaatggaa atgatgatat cttttataca aatggagaat
     9961 taagcatatt taaaaatgtt caacagagtt caaatataat taatgacaca ggtgcaggag
    10021 atgttttttc agcatcagta tttcattatt ggcttgaaaa ctatgaagtt gataattact
    10081 tagaaatggc attgtttaat gcctgttcta cagtgagcac tatatataat acttccatga
    10141 ttaattagca acacatgtaa atggcaacta taaaaatggc tgctatgtca aattggactg
    10201 atgaattcaa tataggaagt taggcaacta aacattgtat aactttcttt ttagttattg
    10261 gagcgtaaag ttttgaacat aataatattc gattgcgcaa atgattgtaa cttccataat
    10321 caaatcatgt acgtttaatt aattttatta tgttagttat acattctaat ggacaatgtg
    10381 agctacaccc agttaagttg acatagaaaa tacgaaaact ttgaacgaag tatttcattg
    10441 atatatgtcc cagatgacat gtttgattaa tgaagttcta tgtggctaca acatataaaa
    10501 aattacacaa gtattatctt tgaccttaaa agcatcccgc catattttta agcattttgt
    10561 gaagtaataa aatatttact gtgttatgat agcatgtact atagagaaat agaggtacta
    10621 ttttatggaa aagttaaatc aagttatgtt atatgttgat gatgttgaaa aagcgaaagc
    10681 attttggaca gaaattttag aatttgttgt tgtaagtgaa acaccgttag ctgaggacta
    10741 tgttgcagtt gaagtatcac ctacgaaaga tgctgaaacg tcattgacga ttatggcaaa
    10801 agaatttatt gaaaagtata gcccggaggt gaacctagga acaccatctt taatgtttaa
    10861 agagaaaaac tttgatgcat tatactcaaa attaaatgat ttaggtttaa ctgggcatga
    10921 tatagtagaa atgaatggtc aacgtgtatt taatttccaa aatggccaag gtaattattt
    10981 tgcagttagt gactaaacaa tcgattttaa tatttataca aacctttata ttgattttcg
    11041 gattgaattt cagtatgaag gttttatttt tatcgtttta tgcaatcgtt tgcacaaatc
    11101 ttttgacttt tttagaaagt gtgactaaac tggtattata caaaggttgg aagcgttttc
    11161 cagtacagag attaacagga ggtgattaaa tggttacgat taaagatgtt gcactaaaag
    11221 ccggtgtttc tccttcgaca gtttcaagag ttataaaagg aaatcaacgt ataagcgaag
    11281 cgacaatttc aaaagtgaag aaagttatgg aagaattgaa ttattttcct aataccgctg
    11341 ctagaacttt aattactaac caaacataga aaattggttt agtgttaaaa gggtctgagg
    11401 agcctattcg actgaatcca ttctacatca atgtattgct aggaatttct gaaacgtgta
    11461 accagcatgg ctatggtaca caaacgacag tctcaaataa tatgaatgat ttaatggatg
    11521 aagtttataa aatgattaaa caacgaatgg ttgatgcgtt tatactgctc tattcaaaag
    11581 aaaatgatcc gattaaacaa atgttaattg atgaaagtat gccatttatt gtgattggta
    11641 agcctacatc ggatatagat catcaattta cacatataga taatgataat atattagctt
    11701 ctgaaaattt gacacgacat gttattgaac aaggtgtaga tgaattaata tttattacag
    11761 aaaaaggcga ttttgaagtt tcgaaagaca ggattcaagg atttgaaaca gttgcatcac
    11821 aatttaatct ggattatcaa attattgaga ctagtaatga aagagaagtt atttttaatt
    11881 acatgcaaaa tctgcatacg cgtttgaagg acccaaatat taaacaggca atcatttcgt
    11941 tagatgctat gttacattta gcgattttaa gtgtgctata tgaacttaat attgaaattc
    12001 caaaagatgt aatgacagca acattcaatg attcttattt aaccgaaatt gcgtcaccac
    12061 ctcaa
//
LOCUS       DAMZPP010000088        12000 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMZPP010000088 DAMZPP010000000
VERSION     DAMZPP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12000
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.088"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(125..949)
                     /locus_tag="POH73_001828"
     CDS             complement(125..949)
                     /locus_tag="POH73_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDI6887768.1"
                     /translation="MIKNNKTVEDTYSTKAIVDSISSSVQMKQVMVQQTPGRYMLKSM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLVLVVLTNSELLTSNFMY
                     FTVGWYYKVVSLNKMMWILLYCFLGNILGGFVLFFLMKYAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVIVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(1152..1334)
                     /locus_tag="POH73_001829"
     CDS             complement(1152..1334)
                     /locus_tag="POH73_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887769.1"
                     /translation="MAQRYIKVLILYIFTTLLSSITVTSKCVPNKVVRFILRTVVGYG
                     IFAYGLHYFSNLKKNK"
     gene            complement(1420..1887)
                     /locus_tag="POH73_001830"
     CDS             complement(1420..1887)
                     /locus_tag="POH73_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDI6887770.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSEFNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(2073..3620)
                     /gene="adcA"
                     /locus_tag="POH73_001831"
     CDS             complement(2073..3620)
                     /gene="adcA"
                     /locus_tag="POH73_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HDI6887771.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHGKEEHHHHGGYDPHVWLDPKI
                     NQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISHE
                     SIGYLADRYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTETI
                     RKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAESK
                     HDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKDL
                     KAYYDKGYKTDITNIDIKGNEITFTKDGTKHTGKYEYNGKKTLKYPKGNRGVRFMFKL
                     VDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQI
                     KEEMLAH"
     gene            complement(3913..4179)
                     /locus_tag="POH73_001832"
     CDS             complement(3913..4179)
                     /locus_tag="POH73_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDI6887772.1"
                     /translation="MVRLNITFSPQAFEDYKYFQQNDKKMVKKINELIKSIDRNGALK
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(4179..4430)
                     /locus_tag="POH73_001833"
     CDS             complement(4179..4430)
                     /locus_tag="POH73_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDI6887773.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSDIVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTVTKDIDV"
     gene            complement(4594..4694)
                     /locus_tag="POH73_001834"
                     /pseudo
     CDS             complement(4594..4694)
                     /locus_tag="POH73_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4701..5300)
                     /locus_tag="POH73_001835"
     CDS             complement(4701..5300)
                     /locus_tag="POH73_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDI6887774.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVIYGDYKC
                     PYCKELDEKVMPKFRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKGAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(5319..5681)
                     /locus_tag="POH73_001836"
     CDS             complement(5319..5681)
                     /locus_tag="POH73_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDI6887775.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDDEVHYYAYDFSDKRVSYKQDFDS
                     RRYYQQHDADYHEENMTN"
     gene            5936..7186
                     /gene="fmhA"
                     /locus_tag="POH73_001837"
     CDS             5936..7186
                     /gene="fmhA"
                     /locus_tag="POH73_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HDI6887776.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKQNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDTLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESAEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPILYKLQTYLKHKRR"
     gene            complement(7283..8014)
                     /locus_tag="POH73_001838"
     CDS             complement(7283..8014)
                     /locus_tag="POH73_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDI6887777.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVGKGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVFVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKAEAREKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAREVSNNIVFIHEGVIGEQ
                     GAPEEIFNNPKTEELKRFLNVINEE"
     gene            complement(8011..8730)
                     /locus_tag="POH73_001839"
     CDS             complement(8011..8730)
                     /locus_tag="POH73_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDI6887778.1"
                     /translation="MFLNLNSEQLHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKVLRSIARVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYRQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(8711..9490)
                     /locus_tag="POH73_001840"
     CDS             complement(8711..9490)
                     /locus_tag="POH73_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDI6887779.1"
                     /translation="MKRLLFVVIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKAEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFNDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSKSAFAF
                     SKKVDDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(9611..11050)
                     /gene="mdeA"
                     /locus_tag="POH73_001841"
     CDS             complement(9611..11050)
                     /gene="mdeA"
                     /locus_tag="POH73_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HDI6887780.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGIVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRKLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIEMMNQIKIDTMLHGYHAG
                     FLFALLITVISFFCSFMLQGKKKEVDSHQ"
     gene            11224..11328
                     /locus_tag="POH73_001842"
     CDS             11224..11328
                     /locus_tag="POH73_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDI6887781.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(11481..>12000)
                     /locus_tag="POH73_001843"
     CDS             complement(11481..>12000)
                     /locus_tag="POH73_001843"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDI6887782.1"
                     /translation="LLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDD
                     ARKEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVR
                     VIPFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYEL
                     TDDLEVIDKYYL"
BASE COUNT         3953 a         2202 c         1653 g         4192 t
ORIGIN      
        1 gggggcccca acacagaagc tgacggcagg ccagcttaca ttaatgtgca agttggggaa
       61 cgatgccccc aaaaaggatg attctaaatt cattgttgtc acaacatctt caattattac
      121 ttcattactg ttgctctaaa cgattgcgtt taccatttaa aaatgcataa acaagtccta
      181 caaaaatacc gccacctaca aagttaccaa ggaatgcaaa aacgatattt tttaatacat
      241 gtaaccaaga tacagcatca aggttgaaga acaccatacc tgcatataaa ccagcattga
      301 atacaacgtg ctcatatccc atgtatacaa agactataac accacaagca atgaagaatg
      361 cttttgtcaa tccgcctttg aattgcatag aaataaaaat accgatatta ataaagaagt
      421 tacagaaaat accttttgta aaaatattca accaagtcga atcgactgtc tttttctgaa
      481 ctaaggcagt taaggcttgt gtcatttctg gtgtcataac atgtgcatat ttcattaaga
      541 agaataatac aaaaccacct aaaatatttc caaggaaaca atataataaa atccacatca
      601 tcttattaag agaaacaact ttataatacc agccaactgt aaaatacata aaattacttg
      661 ttaataattc agaattcgtt agtacaacca agaccaatcc taagctgaaa gcaattgctc
      721 ccattaaatt aatgagacca tcaacatgtg tacttgcgaa ttgtgtttta ataccaaaca
      781 taaaaacagt aacaatggac aataagaaac ctgccatcat agatttaagc atgtatcgtc
      841 ctggtgtttg ctgtaccatc acttgtttca tttgaacaga tgatgatatt gagtctacga
      901 ttgctttagt tgaatacgta tcttcaactg ttttattatt ttttatcaca gcacttcccc
      961 catttactat tatcaaataa attttaccta ttaattatat tagtatttag atgtaattca
     1021 tagattaaaa ttcagaaaat tctttttacc aattcaaaac taatatttac tcataaaaat
     1081 agcgagatag atatcgcgac ataaaacaat tggtatcatt ttaattgcgc tcatctcgct
     1141 ttatgtaagt attacttatt cttttttaaa tttgaaaagt aatgtaaacc atatgcaaat
     1201 ataccgtagc ctacgactgt acgcaaaata aatctaacaa ctttatttgg tacacatttc
     1261 gaagtaacag taattgaaga taaaagcgta gtaaaaatat ataatattaa tactttaatg
     1321 taacgttgtg ccatcgaatc acccttcatt caattaataa tattacgtta ttattataaa
     1381 ctgaatggca tataaaattt ttacatattt tcaacaactt tatttcaata aagcatcaag
     1441 ttgatccaat gtttgattca tcccttgctc aacacccatg tttatgactt gttgagcggc
     1501 ttctttagtt ggaaataccg atgttgatgt cactgtcgtt ttcgttgtat tgaactcgga
     1561 aaatgacaaa gtaatcttca tgcttggcat ttttgtatct tttgcacctt gaggtgtcgc
     1621 aaacgagtca atatattcaa ttaaatatgg acgcttaacc gttttatatt ctgctaatgt
     1681 atagcttgtc attgtgggtg ttttaatcgc gtaaaatgca tcaccgcctg aaacagcatt
     1741 aaagcgaaac actttggtac tagcgtcttt tggatgaaac catttttcaa ataatgcctt
     1801 tgtcgtatag gcatcaaaca ccttttcaat cggtgcttct atcgttcttg aaaaaataat
     1861 tttattatct tcaactttaa tcgtcatctt cccactccct atcttttata tttactcatc
     1921 ttaacatgca ttgagtgaca tatgttacca aatcatttgt tataaactat agtaatatta
     1981 atcattccct ctgttgaaat tgataactac gaaataaaaa acacactcta ttcagctatt
     2041 aacagttgag tagagtgtgc aaacctgttg aattaatgcg ctaacatttc ttctttgatt
     2101 tggtctttat ttaattttga aggataatat gttggccagt tatccatttc ttttaataac
     2161 gcgtcattat cattacccat aaagatatgg aagtgttctg cctttttagg tgcaatgttg
     2221 tgatcgctaa attggatgaa tttcggtaag tctttatcat taccatcgac caatttaaac
     2281 ataaatctca cgccacggtt acctttagga tatttcaatg ttttcttacc attgtattca
     2341 tatttaccag tgtgtttcgt accatcttta gtaaatgtaa tttcatttcc ttttatatca
     2401 atgttagtaa tatcagtttt atatcctttg tcataataag cttttaaatc tttagcagat
     2461 ttcttcggat cattttcagc tttatgttcc atcacttcat caagcgtacc gtcttttaag
     2521 taagggtaaa cagattgcca ttcaccagca taatcgctta attcacggtc tttaacttgc
     2581 tcatctttaa aatacccatc agaaattgct ttgtcgtgtt tactttcagc tttgtcgtct
     2641 ttcactttaa caccactgtc taaagcttta ccgatatttt caatattcga tttcattaat
     2701 gattgatagg taatattatc ttttttctgt tgttctttat ttaaagactc catgttgtag
     2761 aattttaaag gcttcgcatc tgtttcttta cgaattgttt cagtcacttt attcgcaaca
     2821 ttatcttcgt aaagaatata tttagcattg ctatctctaa tttctttaac aattttagtc
     2881 aattcttttt gtgatggatc ttcagcattc atgttttgaa tacctttttg aacaaaacca
     2941 taacgatcag ctaagtatcc aattgattca tgtgaaatga atactgcatt accttgctta
     3001 tctttagtaa cttgcttcat atcgttatca attttcttaa gatcgtcgtt taattttttg
     3061 tagtttttct catagtcatc tttatgtttt ggatctttct tcactaattc atctttaatt
     3121 tctttagcga aagtttggtt aattttagga tctaaccata cgtgtggatc atatccgcca
     3181 tgatgatgat gttcttcttt cccatgatca tgtccctcat gttcatgctc ttcaccatgt
     3241 tcgtgttgat cagttaaaag ctttgcttta tctaatttat cttctaaaga cagtttttta
     3301 tctttatctt taatagtaga tgcaactttc ttagcaaccg gatctaaatt atcccctgta
     3361 tacataaaca agtctgactt gcttgcactt aatatatctt tttgtgttgg ttcatagcta
     3421 tgtaaatctg tccctgctgg atagattgat gataccttca cgtgttttcc accaatttgc
     3481 tctgcaaatg attgcaatgg ataaactgtc gttttaattg ttaccttgcc atctttatct
     3541 tttccatcat cattcccaca tgcggctaat gataaagtta cggctggtac aagaagtaac
     3601 atacctaatt tctttttcat agtcaccctc ctaaatagta atctttacgt tttaaaatta
     3661 tatactcatg ctacttaaaa tacaagcact gtttttataa ataatataaa gtcgaatcat
     3721 actttaggta tttaccactt ttcgttttat ctaatacatg actattgtac aaatatgata
     3781 cagaaaactt tatagtattt acgaacgtcg taatataaga aaaaagacaa tctctatcat
     3841 attgctaata gaaattgcct ttcatattgt cctaaaataa cttaccctat ttttatagtg
     3901 tgacttttag atttaataat ggtatttaca tgatgctatt tttatatgat tgtcatctac
     3961 tgtataaacc aatctatgtt cgtgattgat acgtctacta taatacccag tcagattcga
     4021 ttttaacttt tcaggcttac ctataccttt caacgcacca tttctgtcaa tacttttaat
     4081 taactcatta atcttcttca ccattttttt atcgttctgc tgaaaatact tataatcttc
     4141 aaaggcttga ggcgaaaacg taatatttaa cctaaccatt atacatctat atctttcgtt
     4201 acagttttcc cacgttctag atctgcaatt gattgagcta agtgttcagc attatttgga
     4261 ttctgttgga ggtaaagtgt ttccatcatg gagttataat ctgattctga catgattact
     4321 acgtttttat catcagtaga tgttacagtt actatatcac tatcatcatt tacttttgtc
     4381 ataagtgcct ttaaattctg tcgagcgtat gaataatttt taataatcat atttatcgcc
     4441 tccttttaac acaccccaat tgtacaaata tttgtacaat aaaacaagag acacagaaaa
     4501 aaccactaat caagattttg cacggggaag aatcaatacc tatcctgtac tgattcccta
     4561 ctaaacaatg tttacatgat aatttccacc tatttagact tcatacatta aaaagacaat
     4621 ctctatcata ttgctaatag aaattgcctt tcatattgtc ctaataaatt ggtatcactt
     4681 acttatctca acaattaaga ctatttgatt ttatctttta ataatttttc ataactttca
     4741 taatcatatg gatcttcaac tttctcgcca ttaataaatg cagttggtgt cgtttttata
     4801 tgattatctt tagctatttt tttatctttc tctgcacctt tccaagactt gctatctttt
     4861 gtcttgtaat cttttataat tttattttcc gtctctttat ctaaatgcag ttgtttaata
     4921 tgtttatcta atagttcttt tgttaaccat tctttatttt catcttgctg ggcagcaaat
     4981 aattgctttt gaaaatctaa aaatgattta ggtgcataca tcaatactgc atgactcgca
     5041 cgtgaaccaa caattgagtc tttacctaag aaagctaaat tgacaaattg gtattccact
     5101 ttgtgattat ctatataatt tttacgaaac tttggcatga ctttttcatc taattcttta
     5161 caataaggac atttatagtc gccatatatg acaactaatg gtttgccgtt tttcgaagat
     5221 gtcgttgctg attctttttt accacaagct gttaaaatta acatgctcac tataaataat
     5281 gttagtaatt ttttagtcat aataactcct gttcatatct agttcgtcat attttcttca
     5341 tgataatccg catcatgttg ttgataatat cgtctcgaat caaaatcttg cttatatgat
     5401 acacgtttat cactaaaatc atatgcataa taatgcactt catcatcatt actcaaaggt
     5461 ttataagcta aaatgataat tttatcctta tcatagacat aaatattagc atctttgcgt
     5521 tcaaaatgat caacacgatc accgttgcct tttttggcaa gttgttcttg tttttgattt
     5581 tgaattttaa ctgcttcatc tatttctttc acatatttat cattaccaca tcctgatata
     5641 aagactagta ataataatac cgtcgccaca aatcgcttca atgctcccac tcctactatc
     5701 aacttccaac taactttcaa tataattaat actatacacc gtcaaacatc aatgcgctac
     5761 aactactacc aatacattta atataaatat ccaaagaaat aacagttatg tatagtcaaa
     5821 gaaaaatata tttcattttc tatgtgtatt tgcaatcagt ttttgataaa ataaaaaaat
     5881 agcattatat ataaattcaa aaattacagt tcaattcaga gagggatggg ataaaatgaa
     5941 ctttgtaacg ttgacttcgg atgagttcaa tgcgtttaca acaaagcatt tttcacatta
     6001 cacacaatca gctattcatt acaatcatag agttgattta aaaggcgatg tgcatcttgt
     6061 aggggttaaa gatgacaatg gtcaagtgat tgcaggatgc ttattgacag aagcacgcac
     6121 acttaaattt ttcaaatatt tttatactca tcgcgggcca gtgatggatt atacaaatca
     6181 atcattagta gcatttttct ttaaagcatt aacgtcatat ttaaagaaac aaaattgttt
     6241 atatgtcctt gtagatccat atttaattga aaatttacgc aatgcagacg gtgaaattgt
     6301 taagtcttat gacaaccgag catttgttag aacaatggac acattaggct ataagcatca
     6361 aggctttcca gtcggttatg attcaatgag tcaaatccgt tggttatctg tattagattt
     6421 aaaggataaa acagaagacc agttattaaa agaaatggat tatcaaacga gacgtaatat
     6481 taaaaaaaca tatgatattg gtgtcaaaac taaaacgtta acgattgatg aaacgcaaac
     6541 ctttttcgac ttattccata tggccgaaga aaagcatggt ttcaaattcc gtgagttacc
     6601 atactttgaa gaaatgcaaa agttatacga tgaccacgcc atgttaaagt tggcgtatat
     6661 tgatttaaac gagtatttaa aaacgttaca attaaagcaa caacaattaa cagctgaact
     6721 ctcaggtgtt gaagaggcat tggaagaaag tcctaattca aagaaaaata aaacgaaacg
     6781 cacacagcta gaacaacagc tgaatagcaa taagcgtaaa atcgataata caatagaaca
     6841 aattgaacaa gatggtgcag tgttaaactt agcttctgct ttgtttatct acaatgaaca
     6901 tgaagtttat tacttatcta gtggttcaaa tcctaaatat aatgcttata tgggtgccta
     6961 ccatttgcag tgggaaatga ttaagtttgc taaagcgcat catattgatc gctataattt
     7021 ttatggcatt actggtgatt tttcagagtc agcagaagac tatggcgtac aacaattcaa
     7081 aaaaggattt aacgcacacg tagaagaata tattggtgat tttattaaac caatcaaacc
     7141 tatactttac aaattacaaa catacttaaa acataaacgc cgttaacagt ttatgctgta
     7201 acatctagaa tataacaaag aaccacgcat catgtatatg gcatcatcgc catattaaaa
     7261 catgatacgt ggttctattt gtttattctt cattaataac atttaaaaag cgttttagtt
     7321 cttcagtttt tggattgtta aaaatctctt caggtgcccc ttgttctcca attacacctt
     7381 catgaatgaa tacaatgtta ttagatactt ctctagcaaa acgcatttca tgtgtcacaa
     7441 tgaccattgt catgccctca ttcgctaaat ctttaatcac ctttaacaca tcattcacga
     7501 gttcaggatc aagcgctgat gttggttcat caaacaacat cactttagga ttcatagcta
     7561 atgctctcgc aatggcaaca cgttgctgtt gaccacctga taatgcatga ggtcgttgat
     7621 ctttgacatg tgtcaaacca actttctcaa gtaattctag agatttctca cgagcttcag
     7681 cctttttcaa ctttttaact gtaataagac cttccattac attttctaat gccgtcttat
     7741 gcggaaaaag attatagctt tgaaatacca taccagattg tttacgaact tctatttgtg
     7801 attttttatc tttagatgta tatgttttgc cgttaacaaa tactgtacct tcagttggaa
     7861 tttctaacgc attaatcatt cgtaacaacg ttgttttacc tgacccagat cggccaatta
     7921 aggttacaac ctcacctttg ccaacagata aatcaatacc tttgatgact tcaacatcat
     7981 taaatgattt atggatattg ttcaattgaa tcatgagcga taccctcttt caatataaga
     8041 ttcgtagata ccttggatta ttgaaataat aaagcatacc acccagtaca ttagcgcaac
     8101 taacacataa attgttaaat attcatatgt tgtagacgca acttcttgag cttttctaaa
     8161 catttctgct actaaaataa atcctagtaa tgatgtatct ttgattaaac ttaaaaatgt
     8221 attacctagt gcaggtatag atactcgaat tgcctgcggt aagatgatgc gttgtatcgt
     8281 ttgacgatat gtcataccaa tagaatatgc agcttccgtt tgtccttttg gtatagaaat
     8341 aataccgccg cgaataattt ctgaagcata cgcacctaca tttaatgaca agccaataat
     8401 ggcagcgacc acaggcgcca atgtccattg attatctgca tcattggtta ataatctacc
     8461 taattctgga ataccataaa agatgataaa taattgtact atcataggtg tcccacgaat
     8521 aatagataca tagacacgtg caatacttct taaaacttta ctcgttgaaa tgcgcatcaa
     8581 tgctgtaaat aatgcaatga ttaaccctaa tacaaatgtt acgagggtaa tcggaattga
     8641 atatttcacc aaaccttcga gcataggccc aaaagcttgt tttgcagcat caagtgcatg
     8701 tagttgttca ctatttagat ttagaaacat cttgaccaaa ccatttctta cctattttag
     8761 ctaattcacc gttttcctcg atttttttca agccatcatt gaatttttga actgtttcgt
     8821 catccacttt tttagaaaat gcaaatgcag atttactttg ttcagcgtta cctttgattg
     8881 ccttgatctt agcattaggt ttttgttttt tataatctaa gtatgacaga ctgtcattaa
     8941 atgtaccatc aacacgctta gacaataata aatccattga ttggttaaag ccatcaactt
     9001 ttgtaatatc agcaccttta tccttagcta atttaccata attagatgtg aatgtttgtg
     9061 ctaacttctt acctttaaca tcattaaaat ctttaatatc tttttcattt tcacgaataa
     9121 ctaaaacagc acttgagaat gtgtaaggct tagaaaattt atatttcttt tctctatcag
     9181 gattaatacc cacttggttc gcgattacat caaaacgccc tgcgtctaaa cctgcaaaca
     9241 tagagtccca agaagtttcg ttaaatttaa gctttaatcc ttctgcttta gccactgctt
     9301 taataacatc gatatcataa ccagttaatt tgccatcttt atcatggaaa ctaaatggtg
     9361 cataagtacc ctcagtacca acattaattg tcttactatc tttactagct tccttgtctt
     9421 tagacgaatt gttcccacat gcagctaata tgaaaacgaa agctatcacc acaaataaaa
     9481 gtcttttcat agaatatatc tccttattct tattattcta atcggaataa aaatatttta
     9541 atctgaatcg ccagataagt caatatgttg aacttttgct aaaaagcgtt atatagctag
     9601 caacttatat ttattgatgt gaatcaactt cttttttctt tccttgtaac ataaatgaac
     9661 aaaagaaact aatcactgta atcaatagcg caaataaaaa tccagcgtga taaccatgta
     9721 acatcgtatc aattttaatt tgattcatca tctcaatttt gttcatgcca ttatagtctg
     9781 acatagttgg agaaaagttt ttacttgcat gagataaaat agtaattaat gcagctgttc
     9841 cgatagaacc agcaatttgt tgtactgtgt ttgtcataga tgaaccgtgt gcattcattt
     9901 ctggctttaa ttgattcatc gtatgtgtca ttactggaat taatcctaac gcgataccaa
     9961 ccattcgaat cgcataaact gttgccaaca tgattgttga tgtttgttca tccattacaa
    10021 caaaataagc cgtagtaata acaacaatcg ccatacctac taaagcaagc tttctaggac
    10081 caaacttttc atataaagca cccgaagtca tcgacataaa tgccataaca attgctcctg
    10141 gtaataaagt taacccagaa tctaaagctg aacgatgcaa taaattttgc acaaagatag
    10201 gtaaaatcgt ttccgaacca atcatcgata ccattgtgac acccataata atgataccaa
    10261 ctgtaaattg tttattttta aatactgcaa aatttaataa aggtgttgat agccgatatt
    10321 gacgtaatac aaataatgcg actaaaacaa taccaccaat catagatact aatacgatag
    10381 gattatcaaa tcctttttct gaaattgaac tcgttccata caataacccg ccgaaaccta
    10441 gtacagaaaa aatgacagaa ataaaatcaa taggtacttt aattgtagtt ccaacatttt
    10501 ttatcgtttt aaatccaatt aaaaatgtca cagctgcaat tggagcgaca acatggaaaa
    10561 gcgatctcca gttaaaatat tcaacaagat aacctgaaag tgtaggtcca atagctggtg
    10621 ctaaaccaat taccaaacca aatatcccca tatatttacc gcgttcatgc ggttggaaaa
    10681 catccaaaat cgttgtcatc atcaatggca tcataatacc tgcacctaac gcctgaataa
    10741 ttctagcaac taataatgtt gcaaagtgcg gactcaaacc gccgagtaat gtacctaaga
    10801 aaaagataga aatacccact aaaaacactt gtcttgttgt ataacgttgt ataacaagtg
    10861 cggacaaagg aataacaatc ccattagtca ataaaaaagc cgttgtcagc cattgaactt
    10921 gtgtatatga aatttcaaaa tctttcataa tacttggtaa agccgtcact aatagtgttt
    10981 catttaataa tccaaaaaat ccacccatca gcatagtaat aatcatcgtt aatttttgtt
    11041 tcttcgacat ttaaagcttc cccctcacca tctaaaaaac gacacacatt tttagatatc
    11101 cggtaattat acccattttc aaaaaaaata gcacgacttt attacaattt ctttaaaacg
    11161 taatcattac tatttacata tgagtagtta tcggtcataa tatatttaca aggagggata
    11221 gagatgaaat tagatttaca gactgctcgt cgtaatttaa atagccctaa cattaaaaca
    11281 agaaagcgtg ctttaaagat tattaagcaa cacaaaagag cgaaataatt gttttaattt
    11341 gactatattg tatgtctaac tatattttcg agtatcccta gctcgttaat ataaactcta
    11401 tacttaacta tttccttaca ttacttcaca tatttaagag ctgcatatac actcactcct
    11461 tgtgtacatg cagctctttt ttataagtag tatttatcta taacttctaa atcatccgtt
    11521 aattcataaa caagcggtgc acctgtttta atttcataat taatgatatc ttcatctgac
    11581 acatcttcaa gatatttaat caatgcgcga attgaatttc cgtgtgcaga aactaatacc
    11641 gtttgaccat ctagcaaata ttgtgaaata tgatctgtcc aaaatggtat cactcgaact
    11701 aaagtatctt tcagactttc ggaataaggc atcatacgtt tatctaaatg attatatcga
    11761 cgatcagcta agtaagcttc acgttgttct tcggtttcag caggtggttt cacatcataa
    11821 gaacgacgcc aaatatgtac ttgttcctct ccaaattctt ttctagcatc atctttattt
    11881 aagccttgca atccaccata gtgacgctca tttaaacgcc agcttttata tacaggaatc
    11941 cattgttgtt tagattcagt taaaatataa tgcgttgtat ctaaagcacg tgttaataac
//
LOCUS       DAMZPP010000089        11840 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMZPP010000089 DAMZPP010000000
VERSION     DAMZPP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11840
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.089"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            296..1003
                     /locus_tag="POH73_001844"
     CDS             296..1003
                     /locus_tag="POH73_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDI6887783.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCHKRISLTHFFGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSITYPVAFIIISMTTHIFYFLFRSYIG
                     YGDVLLISALSLFFPLQFTIYVIIFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            1000..1686
                     /gene="radC"
                     /locus_tag="POH73_001845"
     CDS             1000..1686
                     /gene="radC"
                     /locus_tag="POH73_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDI6887784.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIQVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREFFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            1754..2038
                     /locus_tag="POH73_001846"
     CDS             1754..2038
                     /locus_tag="POH73_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887785.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIITGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(2154..2351)
                     /locus_tag="POH73_001847"
     CDS             complement(2154..2351)
                     /locus_tag="POH73_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887786.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            2511..2984
                     /locus_tag="POH73_001848"
     CDS             2511..2984
                     /locus_tag="POH73_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HDI6887787.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKLEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKPENAYQ"
     gene            3058..3177
                     /locus_tag="POH73_001849"
     CDS             3058..3177
                     /locus_tag="POH73_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887788.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNR"
     gene            3377..4219
                     /gene="mreC"
                     /locus_tag="POH73_001850"
     CDS             3377..4219
                     /gene="mreC"
                     /locus_tag="POH73_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDI6887789.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            4219..4749
                     /gene="mreD"
                     /locus_tag="POH73_001851"
     CDS             4219..4749
                     /gene="mreD"
                     /locus_tag="POH73_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDI6887790.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRSFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    4776..5029
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5042..5350
                     /gene="rplU"
                     /locus_tag="POH73_001852"
     CDS             5042..5350
                     /gene="rplU"
                     /locus_tag="POH73_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDI6887791.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            5356..5676
                     /locus_tag="POH73_001853"
     CDS             5356..5676
                     /locus_tag="POH73_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDI6887792.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            5688..5972
                     /gene="rpmA"
                     /locus_tag="POH73_001854"
     CDS             5688..5972
                     /gene="rpmA"
                     /locus_tag="POH73_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDI6887793.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            6459..7751
                     /gene="obgE"
                     /locus_tag="POH73_001855"
     CDS             6459..7751
                     /gene="obgE"
                     /locus_tag="POH73_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDI6887794.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLILFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            7764..8219
                     /locus_tag="POH73_001856"
     CDS             7764..8219
                     /locus_tag="POH73_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDI6887795.1"
                     /translation="MDNKDYKKFYLIRGDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            8233..8835
                     /gene="ruvA"
                     /locus_tag="POH73_001857"
     CDS             8233..8835
                     /gene="ruvA"
                     /locus_tag="POH73_001857"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDI6887796.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTEQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            8867..9871
                     /gene="ruvB"
                     /locus_tag="POH73_001858"
     CDS             8867..9871
                     /gene="ruvB"
                     /locus_tag="POH73_001858"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDI6887797.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKRYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            9873..10898
                     /gene="queA"
                     /locus_tag="POH73_001859"
     CDS             9873..10898
                     /gene="queA"
                     /locus_tag="POH73_001859"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDI6887798.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VSDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSTRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            10921..>11840
                     /gene="tgt"
                     /locus_tag="POH73_001860"
     CDS             10921..>11840
                     /gene="tgt"
                     /locus_tag="POH73_001860"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDI6887799.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENC"
BASE COUNT         4167 a         1605 c         2288 g         3780 t
ORIGIN      
        1 aagttaaatc aaaattagac tttgaagcat tataaaacta aaatagccat gaagaatcta
       61 tttaatcaat aggtagcttc atggttattt tttatgctct aaatcgtatg tgtttataag
      121 gttaatttct tgccattatt aaatttgccg agcagtaatg ggctgttaaa gtatccatca
      181 attatgctta gtaatattga aaaatttaaa tgaaaaagag gttcaatatt tggagaatag
      241 tgtatattta cattttggta ataagtaact ataatttacg taaggaggtt gtcatttggt
      301 agtattgtta tcttatagtt gcagttgtat ttttagtttt ctatatcaat ttatatctat
      361 tgaagaaacg tcatttgatt atttacatag aagatcgaaa tgtgattatt gtaattcatc
      421 actcaaatgg tatgaattaa tgccgattat tagtttttta ttattaaaag ggagatgtcg
      481 aaactgtcat aagcgtattt cgctaacaca tttctttggg gaaacctttg ctttaatacc
      541 tatcgtcttt attaagtatg atttcacata cgtaaatgct acgcttttta taactacata
      601 tgtttttctg cttattttta ctatgaccga tatcacttct ttaatgttag attgtcgctt
      661 aattataatt tattgtattg tttctctctc gttaagtatt acttatccag tagcttttat
      721 cattattagt atgaccacgc atatattcta ctttttattt cggtcatata ttggttatgg
      781 tgacgtttta ctaatatctg cactttcttt gtttttccct ctccaattca ctatttatgt
      841 cattatattt acatttgtca ttgctggttt agttgcttta attaccatga tatttaagcc
      901 gattaaacta ttaccccttg ttccatttat atttatttcc ttttttatca attcactttt
      961 ttataatgat atccatcaat ttttaggagg cgtatatttt tgaaaattaa agaaatggta
     1021 acttcagaaa tgccaagaga acgtttgtta agccatggtg caaaaagtct ttcgaataca
     1081 gaattattag ctatattaat taacaccgga agaaaaggat tctcgagtat tgacattagt
     1141 aatgaactgc ttaaatctgc atcgaatctg aatgaattga aaaaatcttc aattaacgat
     1201 ttgatacaag ttaaagggat tggattacaa aaagcaatta cattaaaagc ggcatttgag
     1261 ttaggggaaa ggatggggag aagagctgaa aataatcgta taaaaataac gcaaccaagt
     1321 gatgttgctg attatatgat tccaacaatg aaagatttaa cacaagaaca ttttgtcatt
     1381 ttattgttaa attcaaaaaa tgtagtgatt aaagaaacct gtgtttttaa aggtacatta
     1441 aatagttcga ttgtacatcc acgtgaattt tttagtattg cggtgagaga aaatgccaat
     1501 gcaatcatcg cagttcataa tcatccatcc ggtgatgtaa cgccctcaca agaagatata
     1561 ataacaacaa tgaggttgaa ggagtgtggt ttgattttag gaatagattt attggatcat
     1621 attataatcg gtgataatag atttaccagt cttgtagaag cgggttactt tgatgaaaat
     1681 gattgaagtt gatagaatta atgacgtatc ttgtgtataa tacctacgaa gtactttcat
     1741 tggaggaaaa atagtgactc tatttattat tatcggggtt ctcgtgccaa tggtttatac
     1801 catgcagtta aatattaaaa atgaacctgt aacaaagcgc aatctattaa taacattagc
     1861 tttatctacg ttaggtattt tagtaacggc attagcaggt gtgattgtta cgaaacaagc
     1921 ttttccttta ttaagtgtag caataggctc aattattact ggaatcgttt ggggcctatt
     1981 actaagtggt agctacgcgc tgatacgatt tttatctaac gcatttgggc gtaaataata
     2041 gtaatctgtt catcaagtag tatacgtgct tgaaaacaaa ttaaactcct aatgtggaac
     2101 tagttaaaaa attctaaact agtacattag gagttttgtt atgcagaata gatttaattg
     2161 ttaaattgaa agtgtggtaa aaatccacta tttatttgaa aaaaatcgag caaccatatt
     2221 aagccttgat atcctaagta aatacataat aaaacaagtc cgacataaat taaaaatcgc
     2281 aaaatagaaa gtccaactct aaaaaggagg atgactaata gtgctattaa aataattgtg
     2341 agtatactca atgctacaaa cctcctcata cgcttttaaa tcataacaaa attataagcc
     2401 attttaaatg aacgtgcatc agtcctaaga tatatcatga ttaaggctga agactgagga
     2461 aaatatacat aataaaatat ataattagat gaagacattg gaggcgaact atgagattga
     2521 tatttagtat gattaaaaac attatagcag taatagcaat tgtgataatt gtttatatcg
     2581 ccttgaaata cgcacctttt ttaaaagaac aagattggaa tcctgtaaat cacgataatg
     2641 atcaaatgaa tcaagttacg caacccacta atgatgccca acatgtttat gtaagtggtg
     2701 agaaatattc attaaaagaa aatgatttaa taaaaaatgt accattaagc caaattaaaa
     2761 atgtatttaa aatgatagat aaactagagt ttatggctgt gtctggaatg aatcgcatgg
     2821 cttataatga tcaatatatt ataggtcaaa gaggagacga atttattctt tataaatttg
     2881 gagatgagtc aatgcgtgtt tacaatactg aatttgaaat gcaacaagac ttaaatgaat
     2941 tagggcaaaa tttacaacta aaacccgaaa atgcttatca ataggaaatt attgcataag
     3001 aaatatattt tagttatcct gtcactagaa taaatttaaa aggttggtgt agttcaaatg
     3061 gcagaacaat caaaacagaa acaagctaat gaacaacaaa aggcccagaa cttattcgca
     3121 cgttggagac aacttcagaa ttcgaacagt gaatcctcga acgatacaaa cagataattt
     3181 taaaatttaa atcgattatt taaactacta gtgactggaa tgtttgctag tagttttttg
     3241 tttttaaaat gccacaaatt tttttacgga gttgttcatt ttcaaagtaa ttcgtagtta
     3301 gtgctttatt tcataatggg agtaatataa aatagaaaag gatatacaat attttgacat
     3361 aatagaggtg ttctgggtgc ttaagttttt taaaaataac aaattaattg ttgttttatg
     3421 tgcaattatc gtttttattg cattaattgg gctgtccata cgttcacaat ctcaatcacc
     3481 tcctgaacaa tatataggtg attctgtgtc ttttggacaa cgagttgtga gttatccagt
     3541 taattttgtt gctggtacga ttggggactt ttttaaaaaa ggagattcta aagaatctaa
     3601 aaataagatt agccagttag aatctaagaa ccaacaatta gaagcggaaa atgaaaaatt
     3661 aaaaaaagag cttgatttaa aagatatttc aaaatttgat cctatttcta ctacggtttt
     3721 agcaagaaat ccggatcagt ggatgaatac aattgtaatt gataagggat ctaaatcagg
     3781 tataacttca aatatggctg tgatgacatc acaaggtttt gttggaagag ttactaaagt
     3841 taataaattt tcttcacaag ttgatttaat ctcaactaat acacgtgcgg gtaaattatc
     3901 tgtaaatata caacacggtt ctaaaaatat atttggctta attgatcgtt atgatgaaaa
     3961 gaactcagaa cttgtaatta gtgatattaa taatagagat aatatctcaa aaggtgataa
     4021 agtcgttaca agtggattag ctgatcaact accaagtaat ttatatatag gagaagtgac
     4081 taaggttcaa aatgatcaat acggcttagc taaagaagtt agggttaaga ctggtgcaga
     4141 cttaacagat ttgagccatg tttatgttgc aaaaagagat cctaaaacaa ttcctgatga
     4201 tgaaagcagg gataaataat gcgtacactg tattattttt tgataggtat tttactattt
     4261 tatatagata ctgcaattgg gcttcttata ccaatgcaca taggtaagtt tgaacttgta
     4321 tttgtaccac accttacatt tatgtatatt ttaatgatgg tagtgtatcg tagctttggc
     4381 gtatcattat tgctcagcat atttttaggt gtaatgactg atgtatactt tggtagtatt
     4441 tatggagtgt acttatttgg ctatatattg tttttagcac ttatagatcg attctttaaa
     4501 attttttaca aagatcattc gatgttattc attattatat tagctagcac cttattatta
     4561 gaagtctatg tggcattaat atacggtatg ttaggattca ttcaatttga tattattcat
     4621 tttgtagtct ttagattatt gccaacatta attatgaatt ttgtactgtt gatcatgctt
     4681 tatccgttga ttataaagtt ccttaaaaaa acaaacaatg acattgacat gaaacgccgt
     4741 caatggtaag atgcaatagt tgagtagtta taagtagcta catacaaccg ctcaaatata
     4801 ggttcaagaa catttttaat gaagaaagcg attttcccac agagtgcaaa cgcttgactc
     4861 atttgcagaa gttattgaaa gaaagcgatt gtcaatcaag gcgttgaagg aagaaacgag
     4921 aagagtttat ctttataaat gactcgagtt gcgaccgaaa caacgtagag tgcaaacgct
     4981 tgactcaata acgaaatgtc acctatgtat ggcgtgactt attatacagg aggtgcaaag
     5041 tatgtttgct attattgaaa caggtggaaa acaaatcaaa gtagaagaag gtcaagaaat
     5101 cttcgttgaa aaattagacg taaacgaagg agatactttt acatttgata aagtattatt
     5161 tgtaggtgga gattcagtta aagttggagc gccaacagtt gaaggtgcaa cagttactgc
     5221 tactgttaat aaacaaggtc gcggtaaaaa aatcactgta ttcacataca aacgtcgtaa
     5281 aaattcaaaa cgtaaaaaag gccatcgtca accatacact aaattaacaa tcgataaaat
     5341 caacgcgtaa ttattatgat tactgttgat attacagtta atgatgaagg caaagtaaca
     5401 gacgttatta tggatggcca tgctgaccat ggtgaatatg gtcatgatat cgtttgtgct
     5461 ggagcttcag ctgtattgtt tggtagtgtt aatgcgatta taggattgac atctgagaga
     5521 ccagatatca attatgacga caatggtggt cattttcata taagaagcgt tgatacaaat
     5581 aacgatgaag cgcaactaat tcttcaaaca atgcttgtgt ctttacaaac tattgaagaa
     5641 gaatataatg agaatattag attaaattat aagtgaggtg cattccgatg ttaaaattaa
     5701 acttacaatt cttcgcatct aaaaaagggg taagttctac aaaaaacgga cgtgactctg
     5761 aatcaaaacg cttaggtgct aaacgtgctg acggtcaatt cgtaacaggt ggttcaattt
     5821 tatatcgcca acgtggtact aaaatttacc ctggtgaaaa tgtaggtcgt ggtggcgatg
     5881 atacattatt cgctaaaatc gacggcgttg ttaaattcga acgtaaaggt cgcgacaaaa
     5941 aacaagtttc tgtatatgca gtagctgaat aattttgtct agttaacacc agaagtgaat
     6001 cttctggtgt tttttacttt ttataaaacg tttttcatta ttattttcat tttgttatta
     6061 aaaaatggga attatagaca ttatactaaa tgtgcaactg cataagagcc cctaatcgct
     6121 aaactataag gggacaaagg aatacagttg cagtataaaa agcaaaaagt atcgaatttg
     6181 gtactcggaa atatcagcaa tttgagtaaa gtaaacttaa aagcaactgc ataagagccc
     6241 ctaattattt acaatatata aggggctaaa aggattacag ttgcagcgtt taaagaataa
     6301 actgtaccac aattggtgtt gagaaatata agtattttaa agcaaagatt tataaaagca
     6361 actgcataag agcccctaat tatttacaat atataagggg ctctaatatg ctataattat
     6421 tgggaaaatg aaaattatat gtaaaagagg tgagatatat gtttgtcgat caagtcaaaa
     6481 tatctcttaa agccggtgat ggtggtaatg gtattaccgc atacagaaga gaaaaatatg
     6541 taccatttgg tggaccagct ggcggtgacg gtggtaaagg tgcttcagtc gtatttgaag
     6601 tggatgaagg tttaagaacg ttattagatt ttaggtatca acgtcatttt aaagcaagca
     6661 aaggtgaaaa tggccaaagt agtaatatgc atggtaaaaa tgcggaagat ttagtattaa
     6721 aagttccacc tggcacaatt attaaaaatg ttgaaacaga tgaagtgtta gcagatcttg
     6781 ttgaagatgg tcaaagagct gtagtagcga agggcggtcg aggtggccga ggtaattcac
     6841 gttttgcaac acctagaaac cctgcacctg acttcagtga aaaaggtgaa ccaggtgagg
     6901 aattagatgt atctttagaa ttgaaattat tagctgatgt aggattagta ggtttcccta
     6961 gtgtgggtaa atcgacttta ttatctatcg tttcaaaagc taagcctaaa ataggggcat
     7021 atcattttac aacgattaaa ccaaatctag gagttgtttc aacacctgat caacgaagtt
     7081 ttgttatggc agatttacca ggtttaattg aaggtgcatc tgatggcgtt ggattaggac
     7141 atcaattttt aagacatgta gagagaacaa aagttattgt tcacatgatt gatatgagtg
     7201 gttctgaagg tagagagcct attgaagatt ataaagtcat taatcaagaa ttagccgcgt
     7261 acgagcaacg tttagaagat agacctcaaa tcgtagtagc taacaagatg gatttacctg
     7321 aatcacaaga taatttaatc ttgtttaaag aagaaattgg cgaagatgtg ccagttattc
     7381 cagtttcaac aataacgcgt gataatattg atcaattatt atatgcaata gcagataaat
     7441 tagaagaata taaagatgtt gacttcacag ttgaagaaga ggagtcagtt ggcattaacc
     7501 gagtattata taaacataca ccgtcacaag ataaatttac aatttcaaga gatgatgatg
     7561 gtgcttatgt ggtaagtggt aatgctattg aaagaatgtt taaaatgact gactttaaca
     7621 gcgatccagc agtacgtcga tttgctcgtc aaatgcgttc gatgggtatt gatgatgcac
     7681 ttagagaacg tggttgtaaa aatggtgata tcgttagaat tcttggcgga gaatttgaat
     7741 tcgttgaata ggagcgtaac atgatggaca ataaagatta taaaaagttt tatttaatta
     7801 gaggagatgt cttgcctgaa tccgtggtta aaacattgaa gattaaagat gccttaaaaa
     7861 gtgatccgac attgtccatt tatgatgccg ttaaacagtt tgatctatct agaagtgctt
     7921 tttataaata tagagaaacg atatttccag tagacgataa aatgcttgac catcgagaat
     7981 ttacattaat tttatatgta actgatattg tgggtatgtt ggcacgtgta ctagatgtta
     8041 tatcaaagtt agaactatct gtattaacga ttcatcaaag tattccaatg gaagaaaaag
     8101 caacaataac attatcactg aatgctaaat ctaaagaaac ttcagtagaa gatgttattg
     8161 gcgctttgag aaatttagat tatgtatcaa aagtagaatt aattagtatg agtatgtaag
     8221 gatgtgccta taatgtacgc gtatgtcaaa ggtaagttaa cacatttata tcctacacac
     8281 gtagttgttg aaactgctgg tgttggttat gaaattcaaa caccaaattc ttatcgtttt
     8341 caaaagcatc tagatcatga agttttaatt catacatctt taattgttcg tgaagatgca
     8401 caattattgt atggatttag tagtgaagaa gagaaagata tgttcttgag tttaattaaa
     8461 gtcactggta ttggtccgaa atcagcttta gctattttag cgacaagtac acctaatgaa
     8521 gtaaaacgtg ccattgaaaa tgaaaatgat acgtatttaa ctaaattccc aggaattggt
     8581 aagaaaacgg caagacagat tgtcttagat ttaaaaggta aagtgaaaat cactgaagaa
     8641 gatagcgatt cattattaca agtagacgct acatcgacgg agcaagatca attcgtgcaa
     8701 gaagcaatgt tagcgttaga agcattaggt tattctaaac gagagcttgc aaaagttgag
     8761 aaaacgttaa ataaaaataa atatgactca gttgatgaag ctgttaaggc aggtcttcaa
     8821 ttagttgtat cttaatttta aatagattaa taggggaagt gttgtcatga atgagcgtat
     8881 ggttgatcaa tcaatgcata gtgaagaaac tgatttcgaa ttgtcgctta gacctacgag
     8941 attacgacaa tatattggtc aaaattcaat aaaaagtaat ttagaagtat ttattaaagc
     9001 ggctaaactt cgtcatgaac cattagatca tgtattgctt tttggccccc ctggattagg
     9061 taagacaaca ttatctaata tcattgccaa tgaaatggaa gttaatatac gtacagtatc
     9121 agggccttca ttagaaagac ctggtgattt ggctgcaatt ttatcagggc ttcaacctgg
     9181 agatgttttg tttattgatg aaatacacag actgagtagt gttgttgaag aagtgctata
     9241 ccctgcaatg gaagatttct ttttagatat tatcattggt aaaggcgatg aggctagaag
     9301 tatccgtatc gacttacctc cattcacttt ggtaggtgca acaacacgag ctggtagctt
     9361 aacaggtcca ctaagggatc gatttggtgt gcacttaaga ttagaatatt ataatgaatc
     9421 agatttaaaa gaaatcatta ttagaacagc tgaggtttta ggcacaggta ttgatgaaga
     9481 aagtgctatt gaacttgcta aacgttctag agggactcca agagtagcca atcgactatt
     9541 gaaacgggta agagacttcc agcaagtgaa tgaagatgaa caaatataca ttgaaacaac
     9601 aaagcacgca ttaggtttac ttcaagttga tcaacacgga ctagattaca ttgatcataa
     9661 aatgatgaac tgtattatta agcggtataa tggtggacct gttggtttag atacgattgc
     9721 cgtaacaatt ggcgaagaac gtattacaat tgaggacgtt tatgagccat ttcttattca
     9781 gaaaggcttt ttagaacgta cgccgcgtgg cagaaaagca acaccattag cttatgaaca
     9841 ttttgcaaag tcgaatgagg agagagaata acgtgaatat tgaagaattt gactatgact
     9901 taccagaatc attaattgct caaacgcctt taaaagatcg tgatcatagt cgtttattag
     9961 tcatggatag agaaactggt gaaatgaaac atttacattt taaagatatc attgagtatt
    10021 ttagacctgg tgatacatta gtgcttaacg atacgcgagt aatgccagct agactttttg
    10081 gtttaaaaga agaaactggt gcaaaagttg aaatgttaat gttaactcaa attgaaggta
    10141 atgattggga agtcttactg aaaccagcta agcgtattaa agttggtaat aaattgaatt
    10201 ttggtaatgg caaaattata gctgaatgca taaaagaaat ggatcaaggt ggacgtatca
    10261 tgcgtttaca ttatgaaggt attttacaag aaagattaga tgaattaggg gaaatgccac
    10321 tgccaccata catcaaagaa cgtttagatg atccagatcg ttatcaaaca gtttacgcta
    10381 aagaaagtgg ttcagcggca gcaccaacag ctggattaca ttttactgat gagttattaa
    10441 ctgaaattaa aaataaaggt gttaatatcg catttgttac attacatgtt ggattaggta
    10501 cgtttagacc ggtgagcgtc gacgatgtga gtgaccacga aatgcatagt gaatattatc
    10561 aaatgacaca agaaacagct gatttattaa atgatactaa gtccaaagga catcgcatta
    10621 tatcagttgg tacaacttca acacgtacac ttgaaacaat tcgacgcgat catgataaat
    10681 ttgttgaaac gagtggctgg actaatatat ttatttatcc aggatttgat tttaaagcta
    10741 ttgatggcca gattactaat tttcatttac caaaatcaac attagttatg ctagtatcag
    10801 cgtttagtac tcgtgaaaat gttctgaatg cttataaaac ggcagtaaat ttagaatata
    10861 gattctttag ttttggcgat gcaatgttaa ttatataaaa agaatgtgag gattttgaat
    10921 atgcctgcag taacatacga acacattaaa acttgtaaac aatcaggtgc gcgtttaggt
    10981 atcgtgcaca caccacacgg ttcatttgaa acacctatgt ttatgccagt tggtactaaa
    11041 gcaaccgtta aaacaatgag tccagaagag ttaagacaaa ttgaagcaaa aatcattttg
    11101 ggcaacacat atcatttgtg gttacaaccc ggaaatgata ttatcaaaca cgctggggga
    11161 ttacataaat tcatgaattg ggatggtccg attctaacag attcaggcgg tttccaagtg
    11221 tttagtttaa gtaatttacg taaaattaca gaagaaggcg tggaatttag acatcatact
    11281 aatgggtcta aattattttt gagtcctgag aaatcaatgc aaattcaaaa tgatttaggt
    11341 tctgatatta tgatggcatt tgatgaatgt ccaccgatgc ctgctgaata tgattatgta
    11401 aaaaaatcta ttgaacgtac aacgcgttgg gcgaaaagat gtctagatgc acaccaaaga
    11461 cctgaagatc aagcattgtt cggcattata caaggtggcg aatatgaaga tttaagagaa
    11521 caaagtgcaa aggatttagt agaattagat tttcctggtt atgcaatcgg aggtttgtca
    11581 gttggtgaac ctaaaccggt tatgtataaa atggttgaac atacagagca gtttatgcct
    11641 aaagataaac caagatattt aatgggtgta ggatctccag atgcgttaat cgaatgtagt
    11701 attcgcggca tggatatgtt tgattgtgtc ttaccgacac gtattgccag aaatggtact
    11761 tgtatgacat cgcaaggtcg tttagttatt aaaaatgcaa aatttgcaga tgatttaaga
    11821 ccgttagatg agaattgtga
//
LOCUS       DAMZPP010000090        11716 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMZPP010000090 DAMZPP010000000
VERSION     DAMZPP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11716
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.090"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(267..1028)
                     /locus_tag="POH73_001861"
     CDS             complement(267..1028)
                     /locus_tag="POH73_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6887800.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYHQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            1047..1181
                     /locus_tag="POH73_001862"
     CDS             1047..1181
                     /locus_tag="POH73_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887801.1"
                     /translation="MIIIIINIAYLYAIIWKLTRLQKIVTSFITKHESSSIGLVENYF
                     "
     gene            complement(1279..2193)
                     /gene="rbsK"
                     /locus_tag="POH73_001863"
     CDS             complement(1279..2193)
                     /gene="rbsK"
                     /locus_tag="POH73_001863"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDI6887802.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKTAEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(2221..2625)
                     /gene="rbsD"
                     /locus_tag="POH73_001864"
     CDS             complement(2221..2625)
                     /gene="rbsD"
                     /locus_tag="POH73_001864"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDI6887803.1"
                     /translation="MKKSAVLNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            complement(2640..3521)
                     /gene="rbsU"
                     /locus_tag="POH73_001865"
     CDS             complement(2640..3521)
                     /gene="rbsU"
                     /locus_tag="POH73_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HDI6887804.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGFPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            complement(3753..4751)
                     /locus_tag="POH73_001866"
     CDS             complement(3753..4751)
                     /locus_tag="POH73_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6887805.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNNMMIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            4902..5081
                     /locus_tag="POH73_001867"
                     /pseudo
     CDS             4902..5081
                     /locus_tag="POH73_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
     gene            5131..5523
                     /locus_tag="POH73_001868"
     CDS             5131..5523
                     /locus_tag="POH73_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887806.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKDNYNKYIQGQKQRVIFENGHFV"
     gene            complement(5652..7028)
                     /locus_tag="POH73_001869"
     CDS             complement(5652..7028)
                     /locus_tag="POH73_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6887807.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAT
                     NFGWQTNFIISIGIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSFICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTSLQPSQYIIAVVIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
     gene            7262..8254
                     /locus_tag="POH73_001870"
     CDS             7262..8254
                     /locus_tag="POH73_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDI6887808.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWTSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDVTGHSAEVSFKEGE
                     VVIKDNPIGVLTNHPDLDWHYSNLRQYINISPYPATAKLLEGVSIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNEIVALKLTDDLINRKDMMIFKPEKHITIRKLNDNQ"
     gene            8580..9530
                     /gene="lytM"
                     /locus_tag="POH73_001871"
     CDS             8580..9530
                     /gene="lytM"
                     /locus_tag="POH73_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HDI6887809.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHKHYFYNCYPKNANAN
                     GSGQTYVNQATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHNGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(9582..10241)
                     /locus_tag="POH73_001872"
     CDS             complement(9582..10241)
                     /locus_tag="POH73_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6887810.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFESNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWAYTY
                     Y"
     gene            complement(10255..11046)
                     /locus_tag="POH73_001873"
     CDS             complement(10255..11046)
                     /locus_tag="POH73_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6887811.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGMGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPNIIYE
                     HLEDMKQHQSIDAINKSYHKHIPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVI
                     LHYRLNQQAALQRLSQYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLK
                     SLLLILIYEIGIVFILFHIQTISHRLFMTFIYALAMGIVYLSIFM"
     gene            complement(11043..>11716)
                     /locus_tag="POH73_001874"
     CDS             complement(11043..>11716)
                     /locus_tag="POH73_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6887812.1"
                     /translation="DSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVF
                     IFALSLFTVLKMNQDTVLKARLKMFHFSKERLLIIRALITWFYTMLWSIVGVVWIVFS
                     IPNTFELYNWPTLAIHLSYYVTFLILWLLIIELLTTGLLNSISKVILAIVILVLSGLT
                     IPTIFLQHIANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLV
                     WRYRQ"
BASE COUNT         3959 a         1889 c         2123 g         3745 t
ORIGIN      
        1 gcaatgcaag ttggggaagg acagaaataa attttgcgaa aatatcattt ttgtcctaat
       61 cccgtgatgt taaaatttta agaaaaaaat aatgccacta actaagtatg tatagtgtta
      121 atgtttgagt atttataaaa gtctttaacc aaaattgatg acctactaat caacgataac
      181 gatattgtta agtagatgat tagtgtcaca aagaaattaa agtaacagtc aaacatagat
      241 ataaagtaca gttagtggca ttatatttag tgctcttttt tagcgacaaa agtaatataa
      301 ttcatatctt tacgcaattt agtcatcgtt ttaaacattt tacaaaacat tggtcgattt
      361 tcttttttta aagcattgtt gataatcttt atagttccaa caataccttc gtcataaatt
      421 aaaccttttg gtgtcattaa actcattgga ccagtatgat aatgtacatg attaaaacca
      481 gcttgatgat ataaatctaa ccagccaagt ttcgtctgcg gtgagacatt gacattaatt
      541 gctgcagata atgatttaac tacatgtgtg gcatgtgatt cattaacgat gacaatatca
      601 tgtgttaaca agataccccc aggctttaag actcggtagt actcgcgtaa tgctttttcc
      661 tttatggcga tgggtaacat tgttaacatt gcttcattta aaacgatatc gaattgattg
      721 tcatcaaagg gcaatttaac agcattcgct tgttgaactt gaatatatga ttcaagacct
      781 gctgctgaaa tattttcctg tgctttttct aatgctttct tatttatatc aacgccttga
      841 atgtgacagc cgtatgtatg agctagataa atagatgttg tgcacatatt acatgccact
      901 tctaacactt gcttatcttg tgaaaatgcc ccttgttgta ttaaccaatc tgttgcttct
      961 ttaccaccgg ggcgtagacg agtttttcct aatttagcta aaaatgtatg accagcttct
     1021 ttggacataa cataacctcc ttttaattga taattattat cattaatata gcatatttat
     1081 atgcgataat ttggaagtta acaagattac aaaaaattgt cacttcattt ataactaaac
     1141 atgagtcttc aagcataggt ttagtggaaa attatttcta atagatgatg ttttataaag
     1201 ataaaaaagc acagccatga tatacgaatg ttgcatgtta tatgctaaac cttcatatca
     1261 tagctgtgtt tgattcattt aaacttgatt tacttcttct agtagaggaa tagatgcttg
     1321 cgcgccgtgt ttttgtacag tgagtgagct cgctttatta ccaaaatcaa tagcatctgc
     1381 taagttatct tgcgacttgt ttaagcgact gacaaatgca ccaataaatg tgtcgcctgc
     1441 agcagttgta tcaatcgcat ttactttata agcttcgatg tgttggcttt gatttttagt
     1501 agcaaaatat gtaccttgct tacctagcgt aatcaaaaca gtcttaatgc ctaaagataa
     1561 aaagtaattg gcattgtctt tcatagattg ttcatttgtt actttaatcc cagataacaa
     1621 ttcggcttct gtttcgtttg gcacaataat atcgattaat gacaataatt cattaggtaa
     1681 tgctttcgct ggtgcaggat ttaatacagt cgtcacacca tgtgccttgg caatttcaaa
     1741 tgcagatata atagccggga tgggtacttc taattgtgca acgacaaagt ctgcattgat
     1801 tatagcgtct tttgcgttaa taacatcttc aggtgtcatc gtcatattcg caccaccata
     1861 aacatagatg gtgttttgtc cttctgcatt cacagtgata aaggcttggc ccgtttttgc
     1921 ttcagctgtt ttgataatat atgacgtatc aatatgagcc gctttaaaat cttctaagat
     1981 gaaatcagca acgccatcag tgccaatttt agtaataaat gttgtgtctg cttgcatgcg
     2041 tgcagtggca atagcctggt tggcaccttt acctccgccg aatgcttttt gtgcttcttc
     2101 aacatgtaat gtttcgcctg gttgtgcata tctttcaact gttaaaaatt gatcgacatt
     2161 cgttgaacct aaaataacaa ctttgttggt catgtgtatg ctcctttcaa gttataattt
     2221 ttaaaaagta acattcgatt ctaatgcaat attagagtag ggcgttgtct caccagtacg
     2281 aatattacct ttatttaatg ggtgagctaa gttacttttc atttcttcgt gaggaatgaa
     2341 aatgatttcg atttccgatg aaatcaattg tttaatttgt tgcaattgtg tagggttatg
     2401 ttcttttatt tcttctgcta agtatatttt ttggatttcc atttcttcta acactgtagc
     2461 taagacatca ataaagcgcg gtaagttttt tgtgacagct aggtcgatac gacgatgatc
     2521 atttggaatt ggcatgccag cgtcattaat tgttaataga tcaaaatgac caattgtcgc
     2581 gattgctttt gaaatatgtt catttaaaac agctgatttt ttcatgacat ctacactcct
     2641 tattttataa atactgtaac agaagcggct actaaaatga gtactaagcc gatgattgta
     2701 ataaccattt cttttgacgt tttatgttgt tttaagaaat aaataccagt taatgtagca
     2761 agcacaacgg atgtttgaga aagaataaat ccagttgcta aaccattcat attaggttgt
     2821 gctgaaataa gatatgttaa ggcaccaaat gcaaagaaga aacctgaaat aatttgtaac
     2881 cacgtaattt tattacggaa tggattctct gctttcatat tcataaagcc ataaatgact
     2941 gcaacaatta ccatacccat tgcttgaggt aaaaaggcag ttaggccatc aatagaagtt
     3001 gcttgcggtg cagctgaata taaccagtat ccaaattcac caattaacag aagtaccact
     3061 gcacgacgta aatttttggc gttacttgct tctttgcgtt cactccaaac tgtcatacgc
     3121 gcaccaatta gaataacgac taaagctgta aatccaatga ttttatgacc aatgcctggc
     3181 caatttccta atgcaaagac accccataaa gatgcgccta ataattggaa tgctgttgtg
     3241 actggcatgg cacgagatga gccgactaat tcgaacgctt taaatgtaat gatttgtccg
     3301 aatccccatc ctgcacctga taataaggcg aatagcaaat tggttccagt aggaaagcca
     3361 cttgatgtga ctacggctaa tataatagcg aagattaacg tacctacagt agcaccgata
     3421 atttgatgta caggtttacc accaaacttt gaagcgactg ttgggaagaa gccccagcca
     3481 attaaggggc ctaacccgat aagtaatgca acaatgctca tgttatacac ctcacgattt
     3541 gtttttagta aaacgtttta ccagtgccat cgtaccattt ttatttgtaa aatgcatgcg
     3601 ttttcataaa agctgaaaac gtttaaatca tatttgaaaa ttcatacatt attattttaa
     3661 tttcatagtg aaataattat gattaaaaac cctcagcatt aaagcaacgc taagggccta
     3721 acaatgatga gtatatttcg gaagatacgt agttagtttg aaagatgata gccagttgtt
     3781 gcacgaattt ttaaagtcgt tggtaattca atcatatcaa tggatttatc taagtgctgt
     3841 aatcgttgaa gtaataaggt taaagatgtt ttgccaatat cagttatagg ttgtgccaca
     3901 gtagttaaag gtggcgagac gtacgctgca taatcaatgt cgtcataacc tattaatgag
     3961 atatctttcg ggatactgat gccatgttca attagtcctc gtaaaatgcc aatagcgagt
     4021 tcatcgttaa tagcgaagat tgcagtggca gattgaacca tgatgtcatc aacaatggtt
     4081 agcccaccac gcttagataa ttcagtatgg acgatttgtg gttctggcaa ttgattcgcg
     4141 cgcaaagtat caacaaatcc agcgacacga gtcgacatat tcgccatcat gtcatatggt
     4201 gcaacaatca tcatattgtt gtgaccgagt tctattaaat gttgtgctgc aagttgtccg
     4261 ccttgatatt catttgtccg aacaaaatct gtatagcctt gatggtcatt ttgatccagt
     4321 acgacataag gtacatgatg tttctttaga tagttattta gggcgtccgg ggatgaaatg
     4381 tattgtgcga taattaatcc gtcaatacct cgatcaatta aatgtttaat attgtcatac
     4441 aaatcagttg ctgtagatgt taaaaagcat aaatcaacat cagatggttt atggtcatga
     4501 atactttgca tcagtgctga gaaaaacgga tttgttaagc taggtaaaat gacgccaata
     4561 gtttgaattt tactgccgcg caattgtttt gcatgtttgt taggggcgta gcctaaacgt
     4621 tctgaaacag catgtacgtt ttttatcgtt gttgcggaaa aacgactatc attatgattt
     4681 aaaatatgtg acacagttgt gactgataca ccagcttctc tagcaacatc tttaattgac
     4741 acttttttca tattatattc cttctcttta ttgtgtaatt aatcatacag ttatataccc
     4801 gattattata ttcatcatat catgataaac atttgatgac atatgaagac gtgaatgact
     4861 tttattgtct acaaacagac gcctatagta tgcgattaag gttgaggcga gagaatatat
     4921 tattaaggtt aatacctttt aatggttatg ataactagaa ttttaaaatg tttattgtga
     4981 tgaaaaatac aaaagtcatt attttaaaga ataaaatatt tgtttttgaa aaaagtatta
     5041 aaaaacataa caaaatgatg tatattgaac tatgtgattg aaaaaatttt taattaaggc
     5101 ttgttaaaac ttaagagagg atgtttttaa atgcaattca aattaaaaga agaagagatt
     5161 attagttttt tagaattgaa atatccagaa aaagagttcg aatatggtcg tttgttagtt
     5221 ggacaacata aacgcgatga tttagatgtt tattactttg gcgatacgtt tttaatgtgc
     5281 acgattattt cattcaagac atttgaaatt aaagaaacag tagaattatc atatgatgct
     5341 gttaatcgta ttgtgttaaa agatggatgg ttatttagaa aaatgagaat agaaacaatg
     5401 caaaaagtgt taaaatacgg tacatctaaa ttaatgttaa ctgattttca aaaggacaat
     5461 tataataaat atattcaagg tcagaaacaa cgcgtgatat ttgaaaatgg ccattttgtc
     5521 taattgatag tgaataataa ttagaataag cgaagaacaa tgaaaaatgt tcttcgctta
     5581 tttttataga atttgatgta tatcatttta aagtgataaa gatatttcga aacataattc
     5641 ttctattata tttaagattt aactgttttt ggaatgatca tgtatgcaga caatgagccg
     5701 agtatcatta atacaatgct gacgatgaat gttacagttg cagctacact tggtgcatag
     5761 tttagttgta acatactgaa aactgtagta cttagtgcta taccaaaggc gccacctaat
     5821 gtaccactca ttttatataa tcctgtagct aaaccaactt tttcatttgg catactgaaa
     5881 attgcaattg taaggccggg tgttgcgact aaaccatttc caattgcgca aatgacaaac
     5941 ccaataacaa cagcaatgat atattgtgat ggttgaagtg atgtcatgct aataatagtg
     6001 atgccgatga cagggaacaa cggaccaatg atgagcatca atttgccacc gaaacgtaat
     6061 gttgcttttt cacctaaacg aatcatcgca actgccacaa tggcatatgg caatgtaaca
     6121 agtccagatt gcgcagctga taaaccaagg tgtgtttgag catatatgaa aaagaccact
     6181 gttacgccta gaccgctatt taaaacaaag ttatttaaaa atgcaccaat gaacggacgg
     6241 ttgcgtaata ctgagaaatc aataaaaggt acttcatgtc gacgttcgat gatgatgaat
     6301 atcagcgtag tgatgataaa aatgctcaga caaatgaatg aaaatgtact aaaccaacct
     6361 tgttcgaatc cttgtgttaa caataatgta aagctaccaa tcataacagc gaaaatcgac
     6421 atacctttgt aatcgaatgg atgacggtgg ctatgttgac ttactttttc aggtgtgcct
     6481 tttagaagta atatggcaat gaaagcaatg cctatactaa tgatgaaatt cgtttgccat
     6541 ccgaaatttg tggcaattaa accgccgata acaccagcta ggccaatgcc cccaacagta
     6601 ctaatcatta gataactaat cgctcgtctt aaatgttctc ctttaaattg attatttaaa
     6661 acgccaactg ttgaaggtaa caagatagct gctgatagac cttgtaaaat tctaccgatg
     6721 atgagcagtg cagtgatgtc cgatataatt aatagaagag atgcaaacat actgattatg
     6781 agacccatgt atgtcattct cagttgtcct attttatcag caatatcacc tgcagctacc
     6841 atgaagatac ctgtggcgaa ggaagttaaa ctaatagata aatttaacac ggcaggagag
     6901 gtttgatacg tttgaccaac gagaggtcct atattaataa atgattgtgc aaacaaccaa
     6961 tatgttaatg cagacaacat aatcgcaata ataatattac tgcgtggtga agattgtgtg
     7021 ttattcacag atgtcactcc ttgtaaaaat taaatataga aagtcatgag atgaggtgaa
     7081 cggatgtata gatgatcgtt cactcacaca taagtcatga tttccttaca tatcatacca
     7141 cagttattga gaatgaatat caattaattg cttgtatgat tgatttttta taatatgttt
     7201 aagtgaaaca gctttatttg aaagtgatta aagtaaatga aggtacagag gagtgagaac
     7261 aatgtgcaca ggattcacaa tacaaacttt aaataatcaa gtacttcttg gacgcacgat
     7321 ggattatgat tatccattag atggttcgcc agcagtgacg cctagaaatt atcgttggac
     7381 atctcgcact ggcacgacag gccaaaccca atatggcttt attggcacag gaacagatat
     7441 ggaaggtttt atttatggtg atggtgttaa tgaacatggc gttgccattt caacacaata
     7501 tttccgaggt tatagttcat atggatcaac acacaaagcg gacgcgatga atattacgca
     7561 aaatgaaatt gtgacatgga ttttgggata tacaacaagc attgaagata tgaaacaaca
     7621 agcatcccaa atacatgttg tagcggtata tttaaatgat atcggtgaag ttccgccatt
     7681 gcattatcat gtttccgatg taactggaca ttcagccgaa gtttcattta aagagggtga
     7741 agtggttata aaagataacc ctattggtgt cttaacaaat catccagact tagattggca
     7801 ttatagtaat ttaagacaat atatcaatat ttctccttat ccagcaacag caaagttatt
     7861 ggaaggtgta tcgattgaac ctttaggcaa tgaagcaggt acatttggat tgccaggtgg
     7921 atttacttca actgagcgct ttgtgagaat ggcatttatg aaagcaaaca ttgctcaaaa
     7981 caatgataaa gaaatggatt taatgaatgc attttattta ttagatgcgg taaatatacc
     8041 gattggaatt gtacgtccgc atgatgctga caatcactat acgatgtatc agaccgtaat
     8101 aaatttaact acaagaacgt tatatattaa gtattatggc agcaatgaaa tagtagcatt
     8161 aaagctcaca gatgatctaa ttaatagaaa agatatgatg atttttaagc ctgagaagca
     8221 tatcactatt agaaagttga atgacaatca atagcgattg aaaatggaat ttggttgata
     8281 tgatttgatg gatgattact atcatacatt agcgttgaac cgcgaacatg gacgaatgat
     8341 tagttgttaa ttttgaaaat ttataaaagc ttaagcgaac gcagtgagag atgtcatttt
     8401 aatatcaatg tatggctatt ttgtaatgac aatgtaatga gtttagtaaa acatttcggg
     8461 aatattaaat agttggaaaa tgagaattaa atcctttact cagttgtcta attcttttag
     8521 tatgtgcagt acagtttaat tgaaaactaa ctttaacttt aatggaggat gttttataca
     8581 tgaaaaaatt aacagcagca gcgattgcaa cgatgggctt cgctacattt acaatggcgc
     8641 atcaagcaga tgcagcagaa acgacaaaca cccaacaagc acatacacaa atgtcaacac
     8701 aatcacaaga cgtatcttat ggtacttatt atacaattga ttctaatggg gattatcatc
     8761 acacacctga tggtaactgg aatcaagcaa tgtttgataa taaagaatat agctatacat
     8821 tcgtagatgc acaaggacat aagcattatt tttataactg ttatccaaaa aatgcaaatg
     8881 ccaatggaag cggtcaaaca tatgtgaatc aagcaacagc aggagataac aatgactaca
     8941 cagcgagtca aagccaacag catattaatc aatatggtta tcaatcaaat gtaggtccag
     9001 acgcgagcta ttattcacat agtaacaaca accaagctta taacagtcat aatggtaatg
     9061 gaaaggtcaa ttaccctaat ggcacatcta atcaaaatgg cggatcagca agtaaagcga
     9121 cagctagtgg tcatgcgaaa gacgcaagct ggttaacaag tcgtaaacaa ctacaaccat
     9181 atggacaata tcacggtggt ggtgcgcatt acggtgtcga ctatgcaatg cctgaaaatt
     9241 caccagttta ctcattaact gatggtacag tagtacaagc aggttggagt aactatggtg
     9301 gcggtaatca agtaacgatt aaagaagcga acagtaataa ctaccaatgg tatatgcata
     9361 ataatcgttt aactgtttca gctggtgata aagtcaaagc tggtgaccaa attgcatatt
     9421 caggtagtac aggtaattca acagcacctc acgtacactt ccaacgtatg tctggtggca
     9481 tcggtaatca atatgcagta gacccaacgt catacttaca aagtagataa tacagtaaat
     9541 cccaagttac gatatcatac gcaacttggg attttttcgt tttaataata ggtataagcc
     9601 cattgcttgt cctctaaaac atgttgataa aaaggatctt ggccaattaa cttgatatca
     9661 tctgcaagtg cttcaacttc atctaaatga tgggtagtta atataattaa acatttagat
     9721 ttcataatgt taagtagttg gtggatgtca tgtctagatt ttaaatcaat accaactgtc
     9781 ggttcatcta aaataagaat tcgaggttga cctagtaaac ctactaatat attaatttta
     9841 cgtttattcc caccggacaa tgtagatact ttggcagacg tatcatcaaa gtttaattgc
     9901 tgtaaatatt cgttgatagt tgtatcgtta attggatttt tacaaagtga tttaaaaaat
     9961 ttaatgtttt cagccactgt catgtgctca aataacgcaa tgtcttgtgg cacataaccg
    10021 atgtgatttt gtatttgtct ttgattccat ttttcgccga aatagttgat agttccatca
    10081 ttagcttttt caataccagc aatcatacga agtaatgttg attttccagc gccattatca
    10141 ccaagtaata cggttaaacg attactttca aaggacatgg ttaaatgatt gaaaatctgt
    10201 ttgttgcggt aacgctttga aaggttatta atttctatca tcagttacgc tcctttacat
    10261 gaaaatgctc aagtatacga tacccatagc aagtgcatat ataaatgtca tgaataatcg
    10321 atggcttatt gtttgaatat ggaataagat aaagacgata cctatctcat aaatcaatat
    10381 aagtagcagt gattttaagt aaaatattaa gctgagtggt tgagacaaat atagactaac
    10441 tgccaatagt accaacaata acaaaatagt atgtgtcatt acataagtac tatatagttt
    10501 gaaacggctt aaatgatatt gtgataatcg ttgcaatgct gcttgttggt ttaaacgata
    10561 atgaagtatt acttgaacag cgctaacaaa taaaatcacc gcaaagatta agctaattga
    10621 aatagagtgt tgtgcttgtt tagtaagcga cacaaatttg attttagatt caggtatatg
    10681 tttatgatag gacttgttga tagcatcgat ggattgatgc tgtttcatat cctcaaggtg
    10741 ttcataaata atgttaggaa tttgcatact taaaatagct tcctttttag taacatcatc
    10801 ttcaatatag ctttcatctt catcgacttt tttaatagtt acatagtcag attgtttaat
    10861 tttattgacg aatgattttg atgcagtggt ttggtctaaa tcttgaatgg taatcggtat
    10921 tttgaagttg tcatgtgcta cacggtaacc cataccaata agtacgagtg cgatgacaat
    10981 ggttgttacg agcaagatgt attgtaacca ttgcttgaac acaacaagtt gtatataagg
    11041 cttcattgac gatacctcca aaccaataca gctaaattaa ttatcaaaag tgcgatgaag
    11101 ctaagataga aactagggtg cagttctaaa atgtagttgt ttaaaataat ttctaacaat
    11161 tgatttgtta caactgcgaa cggttgaata ttgaaaacgc catttgctat atgttgtaaa
    11221 aaaatcgtag gtattgttaa accagataac accaggatga caatagctaa tatgacttta
    11281 ctaatactat tcaacaagcc tgttgttaaa agttcgatga ttaataacca cagtattaaa
    11341 aaggtaacat aatagcttaa atgaatggct aacgttggcc aattatataa ttcaaaggta
    11401 ttcggaatac tgaacacaat ccaaactaca ccaacgatac tccataacat agtataaaac
    11461 catgttatca acgcacgaat gattaataaa cgctctttag aaaaatgaaa cattttcaat
    11521 cgcgctttca atacagtatc ttgattcatt ttcaaaactg taaataaaga tagtgcaaag
    11581 atgaataccg ttgttaaaaa tcctgtaatt gcataataac tgcccgtatc gtataaatga
    11641 atcggttcta agttaaatgc acctgaacgg tttaatcctg taatcagcaa atcagtcata
    11701 acattgatac tgtcag
//
LOCUS       DAMZPP010000091        11642 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMZPP010000091 DAMZPP010000000
VERSION     DAMZPP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11642
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.091"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..82)
                     /locus_tag="POH73_001875"
     CDS             complement(<1..82)
                     /locus_tag="POH73_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1722 domain-containing protein"
                     /protein_id="HDI6887813.1"
                     /translation="MKERGHIEQLWREEKYHVLLHSQQSYQ"
     gene            265..1707
                     /locus_tag="POH73_001876"
     CDS             265..1707
                     /locus_tag="POH73_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HDI6887814.1"
                     /translation="MNYKQSAEDILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPV
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIVAITVTLILSKRKANKEVVE"
     gene            complement(1793..2737)
                     /locus_tag="POH73_001877"
     CDS             complement(1793..2737)
                     /locus_tag="POH73_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDI6887815.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(2783..3406)
                     /locus_tag="POH73_001878"
     CDS             complement(2783..3406)
                     /locus_tag="POH73_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6887816.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGIPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIVFNGPLRIMAENRLHKSNLDSLT"
     gene            3567..4844
                     /locus_tag="POH73_001879"
     CDS             3567..4844
                     /locus_tag="POH73_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDI6887817.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGIFVYIYFMQGIQGFDFDHPNRIAIFVALAILGFVGLILGVMVWLGMKS
                     IPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLISAIVRKHSTKRTEVPS"
     gene            5050..5481
                     /locus_tag="POH73_001880"
     CDS             5050..5481
                     /locus_tag="POH73_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HDI6887818.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            5581..5697
                     /locus_tag="POH73_001881"
     CDS             5581..5697
                     /locus_tag="POH73_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887819.1"
                     /translation="MANYAIYFNNININSIMSLNDNNYQFERVNERNEYRES"
     gene            5708..6742
                     /locus_tag="POH73_001882"
     CDS             5708..6742
                     /locus_tag="POH73_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6887820.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKSERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDTHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            6778..7179
                     /locus_tag="POH73_001883"
     CDS             6778..7179
                     /locus_tag="POH73_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6887821.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSQIMEHIMKYIKNVAVESTYV
                     SLIADYPADKLYAKFGFMPTEPDSGGMYIKY"
     gene            7344..7520
                     /locus_tag="POH73_001884"
     CDS             7344..7520
                     /locus_tag="POH73_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887822.1"
                     /translation="MKIDTLRQAIIYYFVALVLSALMIYLFLTNIERVILYSCVFTVV
                     YWAIYAFDVYRKRN"
     gene            complement(7745..8749)
                     /locus_tag="POH73_001885"
     CDS             complement(7745..8749)
                     /locus_tag="POH73_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI6887823.1"
                     /translation="MTETFKAFVIDKDESGKVTPTFKQLSTTDLPKGDVLIKVHYSGI
                     NYKDALATQNHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIVKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSNQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLKPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            8939..9454
                     /locus_tag="POH73_001886"
     CDS             8939..9454
                     /locus_tag="POH73_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6887824.1"
                     /translation="MAGIIKEISKQDASELVELATRTFYDTFGSYYDDNDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIALAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            9770..10399
                     /locus_tag="POH73_001887"
     CDS             9770..10399
                     /locus_tag="POH73_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887825.1"
                     /translation="MKKLVTGLLALSLFLAACGQDSDQQKDSNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSNSGQTTN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKSQALQQLPNFQTALNAANNEANKFGNGHK
                     VYNDYSIEEHNGNYKYVFSFKDPNVNGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(10553..>11642)
                     /locus_tag="POH73_001888"
     CDS             complement(10553..>11642)
                     /locus_tag="POH73_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6887826.1"
                     /translation="MLPKWLYKIAKQRADNKQLQNLGLYTKQPNVLKDHIVFIGDHYQ
                     YIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPKSEKADELINSARVVLVENDIP
                     ETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFYRAKRYNRWLQFDYVIHSADDI
                     SHFYQTAFPSHQANVLAYGNPKHQYLLQKRNESTTQQQYKKSFKINDQKPVLLYAPIG
                     LVSAQQLPLSDALFKAYHVVVQGVDETMLPEEALVAPKYLSAQDLILMSDVVITDYSN
                     IIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLSKIWYTDRQLLINNLISQAIPV
                     IKYPQIQQKEQPLESISQLILSKMTSNK"
BASE COUNT         3979 a         1637 c         2109 g         3917 t
ORIGIN      
        1 tttggtaaga ctgttgactg tgtaacaata catgatattt ttcttcacgc caaagctgtt
       61 cgatatggcc acgttctttc ataaaattac ctccgataat taatgttaat atacttgcat
      121 gtagtgtagc aataaaaatg aatgcgctat caaaagtgat gtaattgttt atataaaatg
      181 ataatagagt gttgcaaata tttttggaac cggttacaat aaagtggaat aaatggaacc
      241 ggttctaatt aggaggctaa atatatgaat tataagcaat ccgcagaaga tattttgaac
      301 gcgataggcg gagaagagaa tttagatgca atggcgcatt gtgcaacgag actacgatta
      361 gttttaaatg atgaaagttt agtaaatgaa gaggcgctaa acaatatgga tgtagttaaa
      421 gggacatttt ctactggggg acaataccaa attattattg ggtctggtac agtcaataaa
      481 gtatttagtg aactggaaaa attaactgga aaagaagcat caaccacttc ggaagtcaaa
      541 gcacaatctg ctaaaaatat gaatccggta cagcgatttg taaaaatgct ttcagatatc
      601 tttgttccga ttataccagc catcgttgct ggtggtttat taatggggtt aaataacatt
      661 ttgactgcga aagatttatt cttttcaggt aaatcattga tagatgtata tagtcaattt
      721 gctggtttag ctgaaatgat aaatgttttt gcgaatgcac catttacatt attaccaatt
      781 ttaattggat ttagtgcagc aaaacgcttt ggtggcaatc cgtttttagg tgctgcatta
      841 ggtatgatat tggttcatcc atcgctaatg agcgcatacg atttcccaaa agcagttgaa
      901 gcaggtaagg ctattccata ttgggatgta tttggtttgc atattaatca agtaggttat
      961 caaggacaag tcttacctat gcttgtagca gcttatatct tagcctcaat tgaaaaaggg
     1021 ttacgcaaag ttattccaac ggtgttagat aatttgttaa caccattgtt atctattttt
     1081 ataacagcat ttctaacatt ttcatttgta ggtccaatca ctcgacaatt aggttactgg
     1141 ttatcagatg gtttaacatg gctttatgaa tttggtggtg caattggtgg attaattttc
     1201 ggattattgt atgctccgat tgttattaca ggtatgcatc atagctttat agctgtagaa
     1261 acgacattaa ttgcagatgc cactaaaaca ggtggatcat ttatattccc aattgcgaca
     1321 atgtctaatg ttgcacaagg tggtgcagca attgcagcgt tctttattat taaacaaaat
     1381 aaaaagttaa aaggtgtggc atctgccgca ggtatttcag cattacttgg tattacagaa
     1441 ccggctatgt ttggtgttaa cttaaaacta agatatccat ttattggcgc tatcgttgga
     1501 tcaggtattg gttcagcata tattgctttc ttcaaggtta aagcaatcgc attaggaact
     1561 gctggattgc ctggatttat ttcaatcaat ccagtacatg caggatggtt acactacttt
     1621 gttggtatga caatatcatt catcgttgct ataacagtta ctttaatttt atcgaaaaga
     1681 aaagcaaata aagaagttgt agaataaaaa aagaggtatg acagtaatca aagtatcagt
     1741 cattgcaaat gcaataggat gaaactttaa gctgtcatac cttttaattt agttataatt
     1801 tctgttttcg ccataagaaa atactaacaa ttacgacaag aattaatgaa atacctacca
     1861 caataatcca agatatataa ctatggtcat caattggcaa cggcacattc ataccgaaga
     1921 aactaaatac gagcgttggt aacgttaata gtaccgtgaa aagtgttaat gtcttcataa
     1981 tcgtattcat atcattagat aataaggaag cgtatgatgt tgtgatactt tctaagatac
     2041 gttgatgtaa ttctgttgtt tcgatggctt ggttattttc aatgattaaa tcttctagta
     2101 attcttcatc ttcttcaaaa cgtttaattg caggtaaacg gaataatttt ttaataattg
     2161 tatcgttacc ttttaaggca gctaaaaagt atactaaact tttttcaact tccattaatt
     2221 tgaaaagttg tttgttagta atgttatttt ttagttcttt ctcaatacga attctacttt
     2281 tattaagtaa tcgtaagtta cgattataat ggtcggcaat agtagttaaa atttctaatg
     2341 caaatctgct gtgaaatttt aaattaatat cacgcttagg taaattttct aaaaattcat
     2401 tttcagcgtc acaaactgtt acgataatac cgttgccaat aataatgcct aatggaatcg
     2461 ttacaaaaga tagaacgcta cgattagttg aattgacgat aggtaaatca ataatgatta
     2521 atgagtaacc agtatcttcg tcatattcaa tacgggaact ttcttctgag tccaaagggt
     2581 cacgtataaa gtcttcaggt atattatatt gttccataag attttcaatt tcttctcggt
     2641 ctggttctac tacattaatc catgacgctg tatgatctaa tggtgttttt ataatatgtt
     2701 tatctatatc ttcagaatgt ctgaatgaag taatcatgat atatagttcc tttctaaagg
     2761 atttaggaaa agcgagtatt acttaagtta gtgaatcaag gtttgattta tgcaaacggt
     2821 tttctgccat tattcttaat ggaccattaa aaacaatatt atgcacatgt ttagccaatt
     2881 catcaactga aatgggctgt ttgtccatta cccagtattt aataattgaa attgttgctc
     2941 cggaaacgta actgtaaaaa tacatctctg gaatgcctcc gatagaatga ttgatactaa
     3001 tataacgttg catattattt ttaatatgtt cgttgatttt caattctaat tgactcgtgc
     3061 gttccaattg taaaatagta tgataaaact ctaaattatc atgaatatgc tggaaaatat
     3121 ttttgagtat attatttaca aaagtattcg caatgtcttc tattgaagaa ccgcgtaaca
     3181 aatcaaattg agtatatgtc gttagttctg aaatatactc atcttccata tctgcgagta
     3241 acatatattt atcctcgtaa tgtaagtaaa atgtccctct attgatatca gcgcgtgttg
     3301 ttatgtcgcg aatggtaatc ttttctaaat ctttttcttg tagtaattta gtgaacgctt
     3361 gtttaatgga ggactttgtc tttctaattc gcctatcttc tttcaaaaat aaatcaacct
     3421 ccttgtgtta ttgaacaatg tgggtgtatt tgtctattaa taaacgtatt gatgaacatt
     3481 gtgtattgtt tattcccact tacatattat aatagacaac atgcgaaata ttcaacacat
     3541 tgtctaataa ggaggtaatt aataaaatga atatatttaa aaataaatta ttatggattg
     3601 caccaatagc aactatgatt atcttggtaa ttttttcttt agctttttat cctgcatata
     3661 atcctaaacc aaaagattta ccaattggta tattaaacga ggacaaaggt acaacgattc
     3721 aagataaaaa tgttaacatt ggtaaaaaat tagaggataa attattagat agtgattcta
     3781 ataaaattaa atgggttaag gttgatagtg aaaaagacct tgaaaaagat ttgaaagatc
     3841 aaaaaatctt tggagtagct attattgata aagacttttc aaaagatgct atgagtaaaa
     3901 cacaaaaagt agttatggat agcaaaaaag aagaaatgca acaaaaagtt gcttcaggtg
     3961 aaattccgcc acaagtagtt caacaaatga aacaaaaaat ggggaatcaa caagtagagg
     4021 ttaagcaggc taaatttaaa acgattgtaa gtgaaggatc aagcttacaa ggttcacaaa
     4081 ttgcatcagc tgtattaact ggtatgggtg ataatattaa tgctcaaatt acgaagcaaa
     4141 gtttggaaac attaacgagt caaaatgtta aagtcaatgc cgcggacatc aatggtttga
     4201 cgaatccagt aaaagtggat aatgaaaaac ttaataaagt taaagatcac caagcaggtg
     4261 gtaatgcacc attcctaatg tttatgccaa tttggatagg ttcaatcgta acgtctatct
     4321 tattgttctt tgcatttaga actagtaaca atatcgtcgt gcaacatcgt atcattgctt
     4381 caattggaca aatgatattt gcagttgttg cagcatttgc aggtatcttt gtttatattt
     4441 atttcatgca aggcattcaa ggatttgatt ttgaccatcc aaatcgtatc gcaatttttg
     4501 tagcacttgc gattcttggt ttcgtgggcc ttattttagg tgttatggta tggctaggta
     4561 tgaagtcaat tccaattttc ttcattttaa tgttctttag tatgcaactt gtaacgttac
     4621 ctaaacaaat gttgcctgaa agttatcaaa aatatgtata tgattggaat ccattcacac
     4681 actatgcaac aagtgtaaga gaactattat acttgaatca tcatattgaa ttaaatagta
     4741 caatgtggat gtttataggg tttatgattt ttggcgctgt atcaagttta atttcagcta
     4801 ttgttagaaa acatagcaca aaacgtactg aagtgccgtc ataatttaat aaataggaat
     4861 atagctatac aagacgtttg tagcgagcgt caaggtatag ctattttatt tgtatagcac
     4921 ttgatgtaaa gttattttag ctgtcatact atgtgctaaa tcaaattcag ttgtgtcata
     4981 tcttcgcttt tttgtcactg ttataatagg tataatcgga tataatgaaa aggtgaggga
     5041 ggattcaaaa tgcgtaggtt attatattca tttctttttt atatgatcat aggtttattt
     5101 agtggctttt tctatagaga gctaacaaaa gcatatgatt ttactggtac aacacaatta
     5161 tcacttgtac acacacatac acttatttta ggtatgttta tgtttttaat attattacca
     5221 ttggagaagt tatttaaatt atcaagttac tacttattta actggttctt ctatgtatat
     5281 aacataggtg ttatcgttac tataggtatg atggtgacaa aaggattctt ccaagtcact
     5341 ggtaaatcat actctccaga agcatttgca ggctttgcag gtattgggca cacaggtatg
     5401 cttgcaggtt tattgttgct atttttcctt ttaagacaag caattcttaa agaaccacgt
     5461 gatgagtctt ttaaaaaata atatgaacta aaaaatatac acatctttca aaggaacatt
     5521 gttgtttcgt actttgaaag gtgtgttttt atattgtgat gggatagtta ttgataattc
     5581 atggctaact atgcgattta ttttaacaat ataaatatta atagtataat gtctttaaat
     5641 gataataatt atcaatttga gcgcgttaat gaaagaaatg aatatagaga gtcataggag
     5701 gcacataatg aaagacgtta caatcattgg tggcggtccg tctggtttgt atgcaagctt
     5761 ttatgctgga ttgcgagata tgtctgtaag attgatagat gttcaatctg aattaggggg
     5821 taagatgaga atttaccctg aaaaaattat ttgggatatt ggcggcattg cgccaaaacc
     5881 ttgtcatgaa attttaaaag atacaattaa gcaaggatta tattttaaac cggaagttca
     5941 tttgaatgaa cgtgtggtag atattaggaa aaaatcagaa cgtcatttcg aagttgaaac
     6001 agaagcggga gaaatatata catcgaaagc agttatcatt gctattggtg caggtattat
     6061 taatccaaaa caattagatg ttaaaggtgt agagagatat caattaacta atttacatta
     6121 tgttgtacaa agttacagac gtttcaaaga taaagatgta ttaatatcag gtggaggaaa
     6181 tacagcatta gattgggcgc atgatattgc taagattgct aaaagcgtga cagttgttta
     6241 tcgaaaagaa gatgtaagtg gtcatgaagc gatgaaaacg ctggtgacag atttaaatgt
     6301 gaaactatgc ccgaaaacac gtattaaata cttggtcggc aatgatgatg aaacgcatat
     6361 aagtgaagtt gttttggaac atgttgaaag tggtgatacg catacagtta aatttgacga
     6421 tgtcattatt agtcatggtt ttgatcgttg taatacatta ttgagtgaaa catcttcaaa
     6481 gctagatatg catgatgatt gtcgtgttaa aggatttggt aatacgacaa ctagtatacc
     6541 tggtatctat gcgtgtggag atattgttta tcatgatgca aaatcacatc taattgcaag
     6601 tgcatttagt gatggtgcaa atgcagcgaa tcttgctaaa acatatatcc aaccagatgc
     6661 gaatgcagag ggttatgttt caagtcatca tgaagtgttt aaagaagcga ataagactat
     6721 tgtaaataaa catttatact aggttgatat attgtgtgat gaaaagtggg gtaaataatg
     6781 gttaaagtga catatgatat tccgacttgc gaggattatt gcgcattaag gattaatgca
     6841 ggtatgagtc caaagacgcg cgaagcagct gaaaaaggat tacctaatgc cttatttaca
     6901 gtaaccttgt atgataaaga taggttaatt ggtatgggta gagtgattgg cgatggagga
     6961 actgtttttc aaattgttga tattgcagtt ttgaaaagtt accaaggtca aggttacggc
     7021 agtcaaatta tggaacatat tatgaagtat attaaaaatg tggctgtcga aagtacctac
     7081 gtaagtctga tcgcagacta cccggcggat aaattgtatg caaaatttgg atttatgcct
     7141 accgaaccag attcaggcgg tatgtatatc aaatactaaa aatgaaaaat cgtaccgata
     7201 aacatactga gttttctaca cagcttaact tagtatgttt ttgtttttct aagtaatgat
     7261 attaaaaaaa tgtgtgtttt atggtaaagt tcaagttgat atgatataga agtttcgact
     7321 ttgtaactaa gggagttaga acaatgaaaa tcgatacatt aagacaagct ataatatatt
     7381 attttgttgc acttgtgtta tcagctttaa tgatctattt atttttaaca aacattgaga
     7441 gagttatttt gtatagttgt gtattcacag tcgtttattg ggcaatatat gcctttgatg
     7501 tatatagaaa gagaaattaa attttatttt tagacacagg ctgggacata aatcaatatt
     7561 ctatgctcta cgaagttata ttggcagtat ttgattagtg ttgcaagtat gataggggcg
     7621 gagaatacat gattccctaa aatgtattaa agtaaagtgc tatttgatga agtatcagaa
     7681 attttataaa ccaaaagaaa ttaaaaaaga catctcgcat aacgggatgt ctttttcatg
     7741 aatactattt atctacaccg aaatcgataa caatgcggcc tttattttca tgtttaatta
     7801 ctttgttaag ttgttctgga agttcatcaa atgtaataac ttgcttgatc tcatgtaaat
     7861 tttcaggctt taaatctttt gcgagacgac gccaaacgcg ctgtcttaat ttcatagcag
     7921 taaatactga gtcaattcct aaaatgttta cgcctcttaa aatgtgaggg aatacagaat
     7981 tagtataagt attaccggca gtcataccaa taactgcaat tgatccacta tgatttaaac
     8041 gctttgtaac ataattaata ccttcaccac caacagggtc tacacaagct tgccaagttg
     8101 atgatgcgag tggcttttta tgatcatctt caacaggaag tcgatcgata acttctttgg
     8161 caccaagttc aagtaattga ttgctaacat cttgtttacc tgtacttgcg ataactttat
     8221 aaccaagttc attaagcatg agtactgcta aagtaccgac accacctgaa gcaccgcgaa
     8281 cgagtacagg accatcttca atattcattc caactttttc aagtctttca attgctaaac
     8341 cggcagtata accagctgtg ccatatatca ttgattcttc taatgttaaa gtatcaggaa
     8401 gcttgacaat ccattctgat tttacacgcg catattcact aaaaccgcca taatggctga
     8461 cacctaggtc ataactcgtt acaattactt gttctccttt ttcaaagcct ggtgcttcgg
     8521 attcaacaat tgtaccagct aaatctattc ctggaatcat aggatacgat tttacgactg
     8581 cattatgatt ttgagtcgct aaagcatctt tataatttat accagagtaa tgtactttaa
     8641 tcagcacatc tcctttaggt aaatcagtag tcgataattg tttgaaagtt ggtgtcactt
     8701 taccgctctc atctttatca attacaaacg ctttaaatgt ttctgtcaca acgatacatc
     8761 ctctcttttt ctcttttaaa aatacggcca ttttactgga ataaatgtat catttattga
     8821 tgttacacac aatcaataca atttgtaatt gatttgtgtt aatgtatttt taaatagttt
     8881 tggcagaaaa atacataagt aatattttga ttcgattact gtttattgga ggatttatat
     8941 ggctgggata atcaaagaaa tttcaaaaca agatgctagt gaattagttg aattagcaac
     9001 tagaacattt tatgacacgt ttggttctta ctatgatgac aatgattttg atcaattttt
     9061 taaagataat tatactgtgg aaaaatttac acaagagatt aaccatgtag attcatttca
     9121 ttatttttat caagaagatg gtgcgaatgt tggttatata aagatgaata ttaatagtgc
     9181 tcaaactgaa gaaatggggg agacctattt agaagtacag cgcatatact ttttgaaaga
     9241 ctttcaaggt ggtggaagag gttcacaatt gatagcattg gccgaaaaaa ttgcacaaga
     9301 acataataaa cataaaattt ggctaggagt ttgggagcat aatcctcgtg ctcaagcatt
     9361 ttataaacgt catggattta aagtggttgg tgaacatcat tttcaaacag gtgatgtaac
     9421 ggatacagat ttaattatgg aaaaagagtt atagcagtaa cttctgttgt cgataaaaag
     9481 aaattgctgt tgtaatcatt gaatatgtat agaaatgtaa aatgtgtaat aaaattgttg
     9541 agtaaaatga ttaaatgtat aaaaaacata aaattcgcaa aaattagata ataacgatta
     9601 aaatttaggg tataataaaa agaaccatag atgatagttg ttgatattgg gggaaataaa
     9661 acaccatatt attgttgacg tctttggtaa caatccatga aatgatacac atggtagata
     9721 tgtatttcaa gcttattcaa tgaatatcga attataggag gagatatgta tgaaaaaatt
     9781 agttacaggg ttattagcat tatcattatt tttagctgca tgtggtcaag atagtgacca
     9841 acaaaaagac agtaataaag aaaaagatga taaagcgaaa actgaacaac aagataaaaa
     9901 aacaaatgat tcatctaaag ataagaaaga caataaagat gatagtaaag acgtaaacaa
     9961 agataataaa gataatagtg caaacgataa ccagcaacaa tctaattcaa atgcaacaaa
    10021 caatgaccaa aatcaaacga ataataacca gtcaaacagt ggacaaacga ctaacaatca
    10081 aaaatcaagt tacgttgcac catattatgg acaaaacgca gcgccagtgg ctcgtcaaat
    10141 ttatccattt aatggtaata aatcacaagc attacaacaa ttgcctaatt tccaaacagc
    10201 tttaaatgca gctaacaacg aagcaaataa atttggtaat ggtcataaag tttataatga
    10261 ttattcaatt gaagaacata atggtaacta taagtatgtt tttagtttta aagacccaaa
    10321 cgtaaatgga aaatattcaa ttgtaacggt tgattatact ggacaagcaa tggttactga
    10381 tccaaactac caacaataat gttaatgtaa ttatgatgca agttaaaaaa ttaaaacggt
    10441 aaactctata atatgaatta gggtttaccg ttttttgcgt attttaaagt aaaaaggttt
    10501 acgttctata gtacttttaa acctcattca cagccactga ggaatttaag cactatttat
    10561 tagatgtcat tttagacaat ataagttgtg aaatagattc taatggttgt tctttctgtt
    10621 gtatttgtgg atatttaatc actggtattg cctgggaaat taaattatta attaataatt
    10681 gtcggtctgt ataccaaatt tttgatagat gacgccaaat atcttcgtta acaccttgag
    10741 actcaatata ttgactgtga tttggagtgt ataatgctac tgttttgtct atagccatcg
    10801 catcaaatat tatatttgag taatcagtga taacgacatc agacattaga attaagtctt
    10861 gagcactgag atactttggt gcaacaagcg cctcttctgg taacatcgtt tcatcaacac
    10921 cctgaaccac aacatgatag gctttgaata aagcatctga taatggtaat tgttgcgcac
    10981 tcactaatcc aataggtgca tataataaaa ctggtttttg atcatttatt ttaaacgatt
    11041 tcttatactg ttgttgagta gtactttcgt tacgcttttg tagtaaatat tggtgtttag
    11101 gattaccata agccaataca tttgcttggt gactaggaaa agccgtttga taaaagtggc
    11161 taatatcatc agcagaatgt ataacataat cgaattgcaa ccagcgatta taccttttag
    11221 cacgataaaa aggtgtttct atatttttaa taggttcttt actatctaaa aataattgca
    11281 ttattggtgt gccttgatgt aattgaatta aagtaccgtt tggttgcaac gtttcaggta
    11341 tatcattttc aactaaaacg acacgtgcgc tgttaatcaa ttcgtcagct ttttcagact
    11401 taggtgatat gaaatgtggt ccgcgacgat cgtcagtaac aaaataacag gcagtcattg
    11461 gattatgttt aacgaagtaa gtaaataaat attttgaatt acctatatat tgataatgat
    11521 caccgataaa aacgatgtga tcttttaaaa cgtttggttg cttcgtatac aggccgaggt
    11581 tttgtaattg tttgttatca gcacgttgct ttgctatttt atataaccat ttaggtagca
    11641 ta
//
LOCUS       DAMZPP010000092        11640 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMZPP010000092 DAMZPP010000000
VERSION     DAMZPP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11640
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.092"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(294..1241)
                     /locus_tag="POH73_001889"
     CDS             complement(294..1241)
                     /locus_tag="POH73_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDI6887827.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
     gene            complement(1374..2171)
                     /locus_tag="POH73_001890"
     CDS             complement(1374..2171)
                     /locus_tag="POH73_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDI6887828.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGFKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            2556..3248
                     /locus_tag="POH73_001891"
     CDS             2556..3248
                     /locus_tag="POH73_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDI6887829.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            complement(3273..4010)
                     /gene="sdpB"
                     /locus_tag="POH73_001892"
     CDS             complement(3273..4010)
                     /gene="sdpB"
                     /locus_tag="POH73_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HDI6887830.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYIKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            4059..4388
                     /locus_tag="POH73_001893"
     CDS             4059..4388
                     /locus_tag="POH73_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887831.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETSYIINENQVFYYEIITLLNKRPLRQVDYGNKIQYLNFYHTELSNAL
                     FAIKFAH"
     gene            4833..5705
                     /locus_tag="POH73_001894"
     CDS             4833..5705
                     /locus_tag="POH73_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI6887832.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
     gene            complement(6088..7467)
                     /locus_tag="POH73_001895"
     CDS             complement(6088..7467)
                     /locus_tag="POH73_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6887833.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EEVEGHPFKMPGGAVSNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            7747..8103
                     /locus_tag="POH73_001896"
     CDS             7747..8103
                     /locus_tag="POH73_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887834.1"
                     /translation="MLKQILPRATKISVIFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFSIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            8100..8285
                     /locus_tag="POH73_001897"
     CDS             8100..8285
                     /locus_tag="POH73_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887835.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(8381..8572)
                     /locus_tag="POH73_001898"
     CDS             complement(8381..8572)
                     /locus_tag="POH73_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887836.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILAIIGYFLLLQGKL"
     gene            complement(8583..9218)
                     /locus_tag="POH73_001899"
     CDS             complement(8583..9218)
                     /locus_tag="POH73_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6887837.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDEYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(9293..10210)
                     /locus_tag="POH73_001900"
                     /pseudo
     CDS             complement(9293..10210)
                     /locus_tag="POH73_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            10478..11008
                     /locus_tag="POH73_001901"
     CDS             10478..11008
                     /locus_tag="POH73_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887838.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(11074..>11640)
                     /locus_tag="POH73_001902"
     CDS             complement(11074..>11640)
                     /locus_tag="POH73_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDI6887839.1"
                     /translation="DTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVVIGL
                     IFVAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDNVDPKRASTGNEY
                     EDKAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVEDTEYFTHQQMAHGLVKSGKS
                     IQVVVGMTVPQVREAFEQMVEDQSSEDK"
BASE COUNT         4071 a         2111 c         1546 g         3912 t
ORIGIN      
        1 tcacctcacc taaaaatcgc tatactattt ataaataccc tttttctgaa aattaataac
       61 ccaagtttga taaatatcta ctgccattta taaggtaaga tttatcttta aattaaattt
      121 gtaatggaat aatttgtaaa aatcaaatca ggcattaaat aaaatagccc ataaatacaa
      181 agtgttatca ccttctattt acgggctatt aattctattc gttattcttt ttacagctca
      241 ttctatctaa ttaatttgtg tacaaatttg ataacttatt ttcccttagt ttactactct
      301 agattatctt ttaataactt agtactttca gcttttgact gctcactagg aatgaagtag
      361 tacaatccgt cactttgaat gccgccttga ccactcaatt gatgtttatt aattgtgtca
      421 ttagcatctt tataattgct tctaatcgta ttcaaatcac ctaatgttaa atctgtttta
      481 acattatttt gaatttcatt cattagacta ttaaaatgtg taatcgatga tgggcttgca
      541 atcttattag ccatcgcttc aagtacaatt tgctgacgtt gttgtcgacc aaagtcacca
      601 ccagcacctt cttctttacg acttctaata aacttcaatg cttgatcacc atttacatgt
      661 gtttgctgtc cttttgtaaa acgaacacca tcaacagtga atgtatcatt acttactaca
      721 tcaacaccgc cgatactatc tatcatatta tgcaaaccat ccatatcgat tgtcgcataa
      781 tgatcaattg gcacattcat caatttttca agtgatttaa cagccatatt tggtccacca
      841 tatgcatagg catgtgcaat tttttcagta gtaccacggc caacaatttc cgctcttgta
      901 tcacgcggta tacttactat ttcagttttc tttgttttag ggttgataga taaaatcata
      961 atactatcac tacgctctcc gccacccttt ttcttacgat cagcatctga atcgacacca
     1021 aataaagcga ttgtgaatgg atcaccatcg tttaaactca cttttttatc tcttaattct
     1081 gaatgattac gatctaacgg attgtgtatc ttattaccag taataaaaat tttagcagct
     1141 acatacacaa cagctacaat cgctaagatt actaaaatac cgaataccca taaaaatatt
     1201 tttttcggta ggctcatttt acttttagac gaacgtttca atcccaccac tcctttacta
     1261 ttccttacat actttgtctg gtttctctat ttattatata gtaaaataat ttttttacta
     1321 tacttctgta gacgtataac tattttttat cattttttat ctctcgagaa tatctatctg
     1381 tatttttgat aaccaccatt tgcatttaaa attttaagta ccgtttcatg acatgcttta
     1441 ttacttataa taaaaggtgc accctttaaa tgatcaattg ccttaccatc taaagttgtc
     1501 atttttagat tcaatagttc tgcaaataaa aactgtgcag caatgtccca aggtttagga
     1561 tttgtattaa tatgtgcccc aaattgacct tttgccactc gcatagaatc taatccgcaa
     1621 gcaccaacta aacgataact aaatgaggcg tcaaataaat cttgcaccgt atccagattc
     1681 atcacttgtg cattaaacga tataatagcg tcttccaatt ttaacgatgg tggttcttcc
     1741 atcttaaatc cattacaaaa agcaccttct cctcgtattg ctttataaag ctttttatgc
     1801 ggataatcat atacgtacga taacattggt ttaccttcat aaaaatacgc caatataata
     1861 caataatctt cttgctgttt tactaaattg gcagttccat caatgggatc cataatccat
     1921 aaatgattaa tttcattcgt aatcatttca ttactttttt cttccgctaa tagttggtgt
     1981 tccggaaaat gtgttgctaa aaattgttga aattgttgtt gaatctgttt atctacattt
     2041 gtaactaaat caaatcgatg acgcttggtt tctgtagtca tctccataat taattgtgga
     2101 atcacattgt ctatttgttt caaccacgaa catattaact tatctatttg ctgtaatgtt
     2161 ttatctgtca tttcgtccac cacttctcat atcattatca ttttattatt accctatatt
     2221 aaaagaatca acaatacaac tgaagacttc ttcattttat gcataaaaaa atcggctagt
     2281 cacgtgctag ccgacaaata gaaaggaaag taagtaataa atattgaaga tgttgtgatg
     2341 taacttgaac gattaaaagc tatctgttat atagctctac ccctttgttt aatcgctccc
     2401 cctgttacaa gtaatatcat agcacaatct tttttaaaat gtaagcgttt tccacaaaat
     2461 ttttccgatt tttttaaaaa gatattgaaa atgtcctcat tgtcactctt atgttatact
     2521 ttgtgtaata tatcatcttt taggaggtgg ctgtcatgaa taaagctgaa aggcaaaatt
     2581 taataattac tgcaattcaa caaaataaaa aaatgaccgc tttagaatta gctaaatatt
     2641 gcaacgtatc caaacgcaca attttaagag atattgatga tttagaaaat caaggtgtta
     2701 aaatttatgc gcattatggg aaaaatggtg gttaccaaat acaacaagca caatctaaaa
     2761 ttgcattaaa cttatctgaa acacaattat cggccttatt tttagtgctt aatgaaagtc
     2821 agtcgtactc gacattacca tataaaagcg aaatcaacgc aattataaaa caatgtttaa
     2881 gtcttccaca aacacgctta agaaagttgc ttaagcgcat ggacttttat attaaatttg
     2941 atgacacaca acatatgaca ctcccaatgt tgttttccga cattttaatt tattgtacag
     3001 aacgaaatgt gatgttagta gatcataggg ttgatgataa tattaaagcc gaaaacgtta
     3061 tatttattgg ccttttgtgt aaacatggac attggcatgc agtcatttat gacattgctc
     3121 aagacaaaac tgccgaactc gaaattgaaa atattataga tatttcgtat tcattcggta
     3181 agacgattca aaccagagac atatccattg ataactatca tcaattttta aaccccatcg
     3241 attcctaaac tatggagtcg acggggtttt atctacgcat aaatacttat tactgatata
     3301 acgaaagaaa caaaattatt tgctatatgt aatgcaattg ttgaacctag gtttcttcca
     3361 gattttatat aagtgaaaac taatatgatg gatagtatga gatatggacc aaactcaaac
     3421 ggcgactttg catcagtcac atgaataaat gcaaataaga acaccgaaac aatactcata
     3481 gctataaaat taaacttctt acctaattct ccaattaaaa tatgtctaaa tacgatttct
     3541 tcaactattg gacctacaat cacaattaat aagaatgcta caggtaaaaa tgcaggcact
     3601 tcaaacattt tatttagctc aagttcattg gctgtttcac tatattgcaa atgtttaggt
     3661 agaaactgtg tcatatattc atatgtataa attaagatga gagcaataat atacgttatt
     3721 gacaatctaa gccaatattt tttgatatac gcaaaaccag ctcgaagcct tgatggcatc
     3781 acttttaaat gaaataaata aaatgcgcca atcccaatcg tatatgctaa agcttgtgtg
     3841 atagtcgcta caaatatcag attactatcg atttcataat aaccaaacaa aattggtcct
     3901 atgtaagctg caattgtgag tgcataaaat ataacaccta taattggaat tataagcaaa
     3961 tctctccatg ctatatcttt aaacgtgtat ttcttttttt cattttccac tgttatatcc
     4021 tttcctgttt aataattgat ttttggaggt acttctacat gataaacgaa actaagtata
     4081 tgagacaaca aattactaat ttgattcaaa tcattgatac gattaaatat aatactttaa
     4141 tgacagattg gaacattcaa acgcatattt ataaatttaa ccaaatagtt actaatgaat
     4201 tgaataagtt caaaggcttt gaaacatcat atataataaa cgaaaatcaa gttttctatt
     4261 atgaaattat aacactactt aataaacgtc ccctccgaca agtcgactat ggtaacaaaa
     4321 ttcaatatct taatttttat catacagaac tatctaacgc attatttgca attaaatttg
     4381 ctcattaacc tatttttcat aaaatgtcat ttaaacaagt tatttattaa aattcacttt
     4441 attacataaa ttatacaatt aaaaagtttc ttcaaattgt aaagatgcat taatcgagtt
     4501 ataatcataa tgattaagat ggtagattta gtcttatata actacattta ctaagattta
     4561 aatagatgag tacataatgt ataaattggg aatatacagt atgacttacg gtaatatatc
     4621 agtatattga tatattattt gcacatggga gtatgctatt ttttatctat acatcttaaa
     4681 aaaatttaca gttttataat tcaatatcat taaacaaaca acatattcag cttatttttc
     4741 attctcttac gttacaagtt gtaaatacca tcacgttaaa caacaatagc caacgctaat
     4801 aaaatatatt gcgttaaagt ggagtgatta atatgtcaaa cgtactaaca gaaatagata
     4861 gtcaatatcc atatatgact aaaaatgaaa aaaagatagc taaatttata ctaaattcac
     4921 ctcaaaaggt gattaaaatg aggtctcaag acttagcaag cttattagat attagtacat
     4981 catcagtcat tcgattcagc aagaaaatta ctgatggcgg ttttcacgac ttgaaaataa
     5041 atatatctaa atatgttcct aaagcatcat caatttacaa tgttgaatta atgaataacg
     5101 aaagcacaga atctttaaga actaaactcc atacacgtac tacacgcgca cttaatcatg
     5161 caaataacga attaaatgat aaaactattg atcaaatatg tcattgttta aaacgttctg
     5221 agacgatttt tatatatggt tttggcgcat cttttgtagt tgctaccgac ttataccaaa
     5281 agttatcaag aataggtctt aatattcaac ttgttcaaga aacacatatt tttgcaacat
     5341 tattagcaac tcacaattcg aacgatagtg ttattcttat tactaacaat ggtacacaaa
     5401 gtgaaatgca atcaatggtt aaagttattg atgactacca tatacctata atcacaatta
     5461 ctagtacaag ggataatccc gtagcacagg catcaaatat tgttttaaca tatgggaaaa
     5521 ctgatgaaaa tgagatgcat atgggagcaa caacttcatt atttgctcaa atgttcacta
     5581 ttgatatttt atattatcgt tatgttgcat taaattatca cgcatcatta gatttcataa
     5641 cacaatctaa aatggcttta gataactatc gtaaacattt atcaaatatt aactttaaac
     5701 attaacagtc ttatataaat tctttaatca tgtcatataa taatttatgc atcattttca
     5761 aagcttacat gtcttttatc ttgtataaca tacgcatatg accaatgatt tgataaggcc
     5821 attctatttc ttatattaaa aacaaacgca gttgattaac ggattcattc catatatcaa
     5881 ttgcgtttgt tattttaata atgctatttt tttaagccca cagtaaaaac taacgatagt
     5941 attgccactt ataagtaaat tcatactaca tctttgtttt aagctatatt tataaaaaat
     6001 gcgcctactc ataacatttc tttggctatt taactttaat aagctagggc caagtttgtc
     6061 ataaaataag cgcacattat tatcatacta ttttgctttt acattatttt tattatatct
     6121 aataaaataa taaatcgtta caatcgttaa gaagatgaca ccaataagca ctgatattct
     6181 agtttcaaca ttaaatacca taccgaccaa tactaatatt aagaataata ttgttaagta
     6241 gttacttact gcgccaccag gcattttgaa tgggtgacct tccacttctt ctgggtgtaa
     6301 ttttctaaaa cgtaagtgac tgaataaaat cataaaccaa ggaatcatac ctggtaaaat
     6361 agacgcacta tatacgtata cgaaaatact cttcgctcca tcgatatata aaggtaaaat
     6421 tacatttaat aaagcaccaa ttaaaatacc aagagatact gcaacaacag tgtacaatgg
     6481 cacaccattt ctcatgattt tagtaaatat tttcggcatt tcacctttat gagcaagtgt
     6541 ataaatcata cgactcgcac tgaaaatacc tgagttacat cctgacattg ctgcagttaa
     6601 tacaacaaag ttaattaatc cagctgcaaa tgtaataccg attttagcaa atgttgcaac
     6661 aaacggactt ccaatgtctc ctaattgatt ccaaggatac acagaaacaa taacaaaaat
     6721 agcacctaaa tagaaaatta aaattctcca gattacacca ttcactgctt taacgatatt
     6781 tttctgagga tctttagttt cacctgcagt aatacctatc agttccacac cttggtatga
     6841 accaattaca attgataatg caaagaagaa gccaacaata ccatttggca taaatccgcc
     6901 atttgtccat aaattagaaa tacctaccgc atggccacca tttccaaaac caaagaaaat
     6961 aagaccaaaa ccagcaataa tcattaaaat aattgttaca actttaatta aagcaaacca
     7021 aaattcaaat tctccaaacg cttttaccga gaataaattc gctgccatta ataataaaat
     7081 agcaataaca ccaggaatcc aagttggcaa ttccgggaac caaaacttca tatattctcc
     7141 tactgcgatg acctcactca taccaacaac aatccattgg aatatattac tccatgctgt
     7201 catataacct gctgcaggat gtatataatc acttgcaaat gttgcaaatg atcctgttgt
     7261 agggtttaaa taaatcattt cccccattgc tctcatgatt aaaaataaaa agatacccgc
     7321 aattaaatat gcaaggataa ctgatgggcc tgtccattta attgtacttg tggcacccat
     7381 gaaaagcccc acgccaatcg taccaccaag tgcaatcatt tgaatttgac gggcactcaa
     7441 tcccctttgt agttcattgt tctccataac ttcttacccc atctctgatt gatactaaat
     7501 aaatccataa aaaaacttat tgccgtaatt atactataaa ttatcgattt tggtacatta
     7561 atatttgcat accatgcaat cgtttacaga actactatag caggcttttg caactttgca
     7621 aacaattatt ttgtgatttt tctaaaaaat taaaaaagtt gtccatgaac cgttgcgtca
     7681 catacaactt cagctattct cggtaattat gatataattt taataatatt aaaaaggagt
     7741 atatcaatgt taaaacaaat actgccacgt gctacaaaaa tatcggtaat ctttgctgta
     7801 gcatttttca tcataaatta tatcggtatg gaaaaacctg acatcctata tttagttgga
     7861 agaactatta ttgcaacact tgctttcata ctcatttgtc ttacagtatt ttctattatt
     7921 aattctccag agcgcaagat taaactcggt acgactttac cgattgcatt aattattggt
     7981 atcattttcg gcgccatatt tttaacggta caaatcggtg tcatcactgg gttaatcatc
     8041 ggtgtgattg ctacgtttat ttgggaattg attgaaaaaa ataaaggagg tcgttcatca
     8101 tgacaacagc attaattggt gtaattgtat taatcttact aattattagc tttgtgccaa
     8161 actaccgtgc tatgaaatca gcaaaagaac aaggaagtaa tgcaacaaga gctacaatta
     8221 tggtaggcat agatattatc ttaattgtac ttatcatcgt gacgttatta ttaaaatata
     8281 tgtaactatt taaaccaccc tatctcatac ataaacatga acgttctacc atttcacata
     8341 catatggtcg tccatttaat gaggtaaggt ggttttctcg ttaaagttta ccttgtaata
     8401 gtaaaaaata gccaattatt gcgagtatta agtaaatcat tgaaccaaca acaaaagatc
     8461 ttacttgcac gcgaaatggt gtctgtttgt taaatatagg accaatcacc aaatttccaa
     8521 cgcctaaaat tactggacca gctagaaaaa acaatataat gtaacccatc atgatacgcc
     8581 tcctatttcg taaagaaacg cgctacaatt tgatcgtaat caatatcttt tgctacataa
     8641 tctacattag aaaagtcctg tgcgcttgtc attttattcg tattaacaat aacatctaat
     8701 ccagctgcaa tcgcggctgt tgcaccattt acagaatctt caatagccaa acattgtgta
     8761 ggcatataat ttaattgttg tactgcagat aaatataatt cagggtcagg tttaacttgt
     8821 tcaacatctt ctctaccaac aactacctct atatactcgt ctagacctaa tgctttaaag
     8881 gtaggcatta tttccgcttt cacactactt gtggcaatcg ccataggaat gtgacgttgc
     8941 tttaaaaatg ccatcaatga tttaatcgta tcaatcatcg gtaatgttgt actagtaaga
     9001 tgatgttctt cataaagttt atctttattt tccgaaccaa tcgctttaat taaatggtca
     9061 tgcaaatctg tagctgctcc tccaatagaa gaacgataaa aatcaatgct tataggatcg
     9121 gcattatgca tctctaaatg tttattaata acattaaata aatgttgttc cgtatctatt
     9181 attgttccat cgaaatcaaa tataactgct ctatacatat atttactcct ctattttttc
     9241 tctttatcaa agcttaacac agtccgatga ccacttcatt ctattagtgc aattataact
     9301 tttttgacca atactttgct aaaataggcc ctgttatatt atgaatgaaa ctaaacactg
     9361 cgcctggtac tgctgcaatt ggattaaaat gcaatgctgc taatgacaca gctaaaccag
     9421 agttctgcat tccaacttca atactgactg ctttttgatc ttgtcgatct aattttaaaa
     9481 gacgagctaa ccaatatcca atcgtatacc ctaatacgtt atgtaacata actactaaaa
     9541 atattaacaa tcctgtagtt aagatttggt gcttactgcc acctacaact attgctaaaa
     9601 ttaatgaaat agcaacaact gatataatcg gcaaagctgt agaagctttt tcagcaattt
     9661 tacgattaat aatttacaaa acaataccta aagcaattgg aattaataca acttgaacaa
     9721 ctgaccacaa catactcagg aaagatactt ccaaccattc atttgcaaat agatatatta
     9781 acgcaggtgt aacgaatggc gctagcaacg tagagaccgt cgtaatagaa acagaaagcg
     9841 ctacgttagc tttggctaaa taactcatta cattacttga tgtcccaccc ggacaacatc
     9901 caactaatat tacgccaaca gcaatatcag gtggtaaatg aaaagacttt gcaattataa
     9961 atgctaatgt gggcataata ctgaattgta ggcagacacc aataattact gctctaggtg
    10021 ctttaaagac cattttgaaa tcattaggtg taattgttaa tcccatacct aacataacaa
    10081 taccaagtaa ataaggtacc cacttaccta atgtcgcaac atgttgtggg aaaataaatc
    10141 caataacagc tgctactaac atccatacca aaaaactctt cgtggcaaat atactcaatt
    10201 tttgaaacat tgttaacaca tcctcgtcga taatattgaa aaatattacc ataagaccag
    10261 taaggttaca attacttttc tgaaaattga gattatttag ccattctctt tttattgcaa
    10321 atattttatc actgataaac aattatttta aacttcattc aaaagtctta gtgaataaag
    10381 aaaaaaagcg tggtaaaatc aacttgaaca aaattaagtc acctttataa atattaaacg
    10441 tgtatttaat gccatgtatt cctcgggagg tcctcacatg ttaattgata aattcgaaac
    10501 atacatcatc aacatcgccg gtttaaatga ccgtactact agaaaaaaat taagtaagtt
    10561 atgcaaaagt gtacaatttt gtgatgcgtt gcaattctca ataaacaaac agtttaatca
    10621 gtatgtatta gaaattagct taccaaagca acaacttcca tacttcattt catttttaag
    10681 ttttcatcag tatagtattt tccaagtact ttcaccaaag aaaataaatg aattacttga
    10741 ttctgataat ttatatcaat ctgcaaagcg tttcgatatc aatattgatg gcttgcaaga
    10801 tgctttcatt aaagacaaag tcatcgatat tatgaatatg ttccaaaatc acactgatat
    10861 cacttatacg ttgaataagt ctcatgcgca tattatatgt acgccagaaa tattcgctaa
    10921 attgttacat acgattgcaa ctagaaatat cgacattcta tctgccaatt atagatcgtc
    10981 atctatgagc aaagctcgta tatcataata aaagacacat ctatacgatg atcatttaca
    11041 atgattgcgt atagtgatgt gtcttttttt gtattattta tcttcggacg attgatcctc
    11101 aaccatttgt tcaaatgctt cacgaacttg tggtactgtc atacctacta caacttggat
    11161 actcttacca ctctttacta atccgtgtgc catttgttga tgcgtgaaat attccgtatc
    11221 ctcaacttta ctttcatcat aaacagttaa gcgtaatctt gttgtacagt tagtaacatc
    11281 tttgatattt tctttgccgc ctaaaccgtc taggtaatac gcagctttgt cttcgtattc
    11341 attaccagtt gaagcacgtt tagggtcaac gttatcacct tttttatttt tatagtcttg
    11401 tttagagaat aacttaacct cttcttctgt tttcttacgt ccaggtaatg gaatatcaaa
    11461 tttcaaaatt aagaatctga ataagaagaa gtaaatcgct acaaatatta agcctataac
    11521 aacttgtgct acataagtgc cccaatgttc tttcccaagt ggtaaccagt ttgttgaaat
    11581 aaagtctaac aaaccaccac ccatgttacc tacaacacca aatgcgtaca ttaaagtatc
//
LOCUS       DAMZPP010000093        11528 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMZPP010000093 DAMZPP010000000
VERSION     DAMZPP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11528
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.093"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..660)
                     /locus_tag="POH73_001903"
     CDS             complement(<1..660)
                     /locus_tag="POH73_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6887840.1"
                     /translation="MEATIAKLDKVVKTYNKHHVLNEISMSINKGELLGLIGPSGSGK
                     TTTIKCLLGMEKIDSGSAEIFAHKMPNRKALNRLGYMGQTDALYENLTAHENLEFYGR
                     LSGLKGNILKDNIEKHIVLVNLEQALNKKVAQFSGGMKRRLSLAITLLAEPELIILDE
                     PTVGIDPKLRQQIWQQFKQMTKDGKSVVITTHVMDEAERCDKVGLIVDGRIFAIDTPT
                     NL"
     gene            794..1357
                     /locus_tag="POH73_001904"
     CDS             794..1357
                     /locus_tag="POH73_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDI6887841.1"
                     /translation="MDQTAFTDSRVTKTINRIHDGMVNLLKTKAYNDITVKDICEKSQ
                     ISRTTFYTHYKTKDDFIYSYLNTLLKEAKKKFLKSETLTQAIFFKKMIYFWLSEGQLI
                     LMLLGDESAYKLHQVIKKSLQQRIEINFVPVLNTKMLTTKEKYFLLIFMCNAIIGVLQ
                     DWVKRGYKESPKEVAEIMNKIFENAFR"
     gene            complement(1534..1989)
                     /locus_tag="POH73_001905"
     CDS             complement(1534..1989)
                     /locus_tag="POH73_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HDI6887842.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWGITKATIVHFITTYVGFLPLAI
                     LAGWFPLTINYLIIFTIIFIVVYTLIWIIQFFKNKNYVDTINEQLKQLK"
     gene            complement(1986..2429)
                     /locus_tag="POH73_001906"
     CDS             complement(1986..2429)
                     /locus_tag="POH73_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6887843.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDHVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRFLKSIKERLSI"
     gene            complement(2592..3965)
                     /gene="hssS"
                     /locus_tag="POH73_001907"
     CDS             complement(2592..3965)
                     /gene="hssS"
                     /locus_tag="POH73_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HDI6887844.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDHKGHKTFYGEPFREDTLSQNA
                     INNVLNNKDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTNDGSIAVFMRPDIGETFS
                     EFRTFLAVLLILLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVDQLIKDIIRHEQFAADEKS
                     LIILADLESIDFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNAYVTTFTI
                     TLPNHSH"
     gene            complement(3958..4632)
                     /gene="hssR"
                     /locus_tag="POH73_001908"
     CDS             complement(3958..4632)
                     /gene="hssR"
                     /locus_tag="POH73_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HDI6887845.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAFTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            4768..5823
                     /locus_tag="POH73_001909"
     CDS             4768..5823
                     /locus_tag="POH73_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6887846.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQESYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYSHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVNEKDLTD
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            5823..6488
                     /locus_tag="POH73_001910"
     CDS             5823..6488
                     /locus_tag="POH73_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6887847.1"
                     /translation="MALVVKDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRSSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSKRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(6718..7686)
                     /locus_tag="POH73_001911"
     CDS             complement(6718..7686)
                     /locus_tag="POH73_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDI6887848.1"
                     /translation="MLLNLLLIFILIVICPLILNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRNRSYNKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPIPEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
     gene            7881..8336
                     /locus_tag="POH73_001912"
     CDS             7881..8336
                     /locus_tag="POH73_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6887849.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            8576..9958
                     /locus_tag="POH73_001913"
     CDS             8576..9958
                     /locus_tag="POH73_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDI6887850.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANITVTLKGDTKLNDSSK
                     KVTINDREMAFSSSKTYGPYPQNKDITISASGKAKGKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLVNENGKQVDKEYELEQGPQD
                     RLQLIKTSEK"
     gene            9989..10116
                     /locus_tag="POH73_001914"
                     /pseudo
     CDS             9989..10116
                     /locus_tag="POH73_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10226..11434
                     /locus_tag="POH73_001915"
     CDS             10226..11434
                     /locus_tag="POH73_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDI6887851.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGIASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
BASE COUNT         4081 a         1571 c         2009 g         3867 t
ORIGIN      
        1 taaattggtt ggcgtatcta tagcaaatat tcttccgtcg acaataagtc caactttatc
       61 acaacgttcc gcctcatcca taacatgtgt tgtgatgacg acactctttc cgtctttggt
      121 catttgcttg aactgttgcc atatttgttg gcgcaattta gggtcaatgc ctacagttgg
      181 ttcatctaat atgattaatt ccggttcggc caacaatgtt attgctaaag ataatcggcg
      241 tttcatgcct cctgaaaact gtgcaacttt tttatttaac gcttgctcta aattcactag
      301 aacgatatgt ttttcaatat tatcttttaa tatatttcct ttcagacctg acaaacgacc
      361 gtaaaactct aggttttcat gagcagttaa gttttcgtat aatgcatcag tttgccccat
      421 atagcctaat cgatttaaag ctttgcgatt aggcatctta tgagcgaaaa tttctgcaga
      481 tccagaatct atcttttcca tacctagtaa acactttatt gttgttgttt taccagaacc
      541 actaggacct attaacccta gcagttcacc tttattgatt gacatactaa tttcgtttaa
      601 tacgtgatgc ttgttatacg tttttacaac tttatccaac ttcgcgatag ttgcttccat
      661 gataacccct ccaaaattga gatacatttc actttattta tatatcatca taagcgtata
      721 ttaagattgc gaataaaata ttaatagata gtgaacatat ttgtttttag tgttcagaaa
      781 aaaggtgggg catatggatc aaacagcttt tacggatagt cgtgtaacga aaacgataaa
      841 tcgcatacat gatggaatgg taaatctatt aaaaacgaag gcatataatg atataactgt
      901 taaagatata tgtgaaaaga gtcaaataag tcggacaacc ttttatacac attacaaaac
      961 taaagatgat tttatataca gctatcttaa tacattgtta aaagaagcaa aaaagaaatt
     1021 tcttaaaagt gaaactctga cacaagcaat attttttaaa aagatgattt atttttggct
     1081 aagcgaaggt cagctcattt tgatgttatt aggtgatgag agtgcttata aattacatca
     1141 agtcataaaa aaatcactac aacaacgtat cgaaattaat tttgtaccag tattaaatac
     1201 aaaaatgtta acgacgaagg aaaagtattt tttacttatt tttatgtgta atgcaatcat
     1261 tggtgtgtta caagattggg tcaaacgagg atataaagag agtcctaaag aagtagcaga
     1321 aattatgaat aaaatatttg aaaatgcatt tcgataggtt tttatcatga tgagttgatg
     1381 tcgtaaagga cagttgcagt tacaatttaa tttgaaaata gtaaaaatat attattcagt
     1441 tcatgcgtat ttaaaataaa tacgacataa agctatacat tgcagaagag actactttaa
     1501 tgcaatatat agcttttata attttaataa aaatcatttt aattgtttca gttgctcgtt
     1561 aatagtgtct acgtagttct tgtttttaaa gaattgaata atccaaatca atgtgtaaac
     1621 cacaataaag ataattgtaa atatgattag gtaatttatt gttagtggaa accaaccggc
     1681 aagtattgct aaaggtaaga atccgacata cgttgttatg aaatgcacta tagttgcttt
     1741 agtaatgccc caatctgtgt atttaaagat aaaatctcca aggaaaaaga cgacgcctat
     1801 gagtaaccat aaaatgatag aaatcaacat tacggtagtt tctgtgaaat gcgtataata
     1861 caatatgcca atagttgatt gtgggttcag tggataatat ttgccgtctg caaataacat
     1921 actaaagaac agtgaaaggg ataaaccaat gattaagcta ataaataatg agtttttcaa
     1981 atttttcata ttgataagcg ctcctttata gattttaaaa aacgtctaga agaataggtg
     2041 tagtgtgcat ctttaagata catacgtata agtccatttg gctctaataa taatttttca
     2101 atgtaatact tgttgacgat ttctgatttg gaaatgcgaa tgaaatgttg tggtaattgt
     2161 ttttctagtt cataaagtcg taattttagt ttgaactttt gattgcttgt aatggctgtt
     2221 acatttttat taataacttg gaatgttata acatctgata cattaatgat atggatttct
     2281 ttgtctatgt atccgactaa tgtatgtgat ttgtctaaat cattgactgc attaataata
     2341 ctttgaacgt gatcattcat tttaggtgca tgtatatcaa tataagattc cgtctcattt
     2401 gcattgataa ataaattgag tttcatcata ggttaatgcc tccttcaaaa ttattaaacc
     2461 ataaacgacc attgatatat ttaaaatgtg ttgaatggta gaaattaaat gttaagtggc
     2521 tagaaagcgc taatcaatat aaaagatacc tcctgaaata aaaacagaaa tgttctttca
     2581 ggaggtagag attaatgtga atgatttggc agtgtaatag taaaggtggt tacatacgcg
     2641 ttactttgtg tgaattggat tgtaccatga tgcaattcaa tgatggattt tgtaattgcg
     2701 agacctaaac cattactatt atcatgtttg ctcactttat agaaacgttc aaataaacgt
     2761 gcttcagctt gtggactaat tggtgaacca tcattactta ttgtgaaaat gatgttgttg
     2821 tgactatgtt gcaaagcgat gtcaatggca ccaccaacat ctgtatactt aatagcattt
     2881 attaataaat tactcaatgc ttgatgtaac aaacgttgat ttcctaggaa atcgattgat
     2941 tctaggtcag ctaaaatgat taacgacttt tcatcagcag caaattgttc atgtcgaatg
     3001 atatctttaa tgagttgatc gacttgaatc ttatcatcga ataataaatg ttggtgatta
     3061 tctaattcgg ataagagcaa caattcagtt gttaatccgc tcagctgcgt tgtaatagta
     3121 taaatatcat ttatatattg ttgtcgtaat gttttatcag aagtttgttg taactcgctt
     3181 aataaatgat gaatatgtgt taatggtgtt ttgatttcat gagatacatt ttgtacaaaa
     3241 tgctgcctca tttgatcaac ttgtcccaat gactctctca tcttattaaa gtgatactgt
     3301 aatgttccaa tttcatcttt gcgtgtttgt ttgataggtg tttcaaaatc accatcaatt
     3361 aagcgttcgg tcgctagctt taactttttt actggacgaa tgattgaata ggttgatgcg
     3421 ataactaatg aaattgagat aaataataac aatattaaca agacggctaa gaaagttcta
     3481 aactcgctaa atgtttctcc aatatctgga cgcataaata ctgctattga accgtcgttt
     3541 gtcttaaagt taataccaac agtattatct gttacattgt cgaaaaatcc agttacgaat
     3601 aatgcgaatg gtttatcttt aataccatgg taatctttat tatttaacac gttgttaata
     3661 gcattttgtg ataaagtatc ttctctaaaa ggttccccat aaaatgtctt atgacctttg
     3721 tgatcaatgg tcattatttg gtaattcatt tgccctaaat gtttgaaata ttgttgaatg
     3781 tgcgttggtt tagctgattg ttcatattgt cttgcttctt taagcgtctt catgattttc
     3841 gcgtcattag atgcttttaa attataatga tagtaaacat ttgttaatac aaaacttatt
     3901 aatgcactaa ataaaataac cgtaatggaa taaatcgcaa ttctagcata gagtgtttta
     3961 aacatgattc tccaccttat agccttgtcc tcttactgtt tcaattgtaa gtgtggcatt
     4021 taattttttt aatctttggc gtagtcgctt aatatgaacg tcaactgttc gttcatctcc
     4081 ttcataatca tagccccaaa ttttttctat tatctgttcg cgagtaaaga tttgcttagg
     4141 acgtgctgca agcataaata ataattgaaa ttccttgttc ggtaacgtca tcgttttatt
     4201 agatacttgg agttccaaat aggattggtt tagcgttaag ttgccaatgg tcatttctga
     4261 atttgaattg atattatatc gacgtaatac cgcacgaatt ctaaaaataa gttccttaac
     4321 ctcaaagggc ttggttacat aatcgtcagt accacttata aacgcacgct ctttgtcact
     4381 aagtgcatcc cgtgctgtta acataataac tggtatatca taatcatttt ttaatgtatt
     4441 acataattga aagccgtcca taccatccat cataatatct accactgcaa tatcgacacg
     4501 ctgtttttct aaaagtttta atgcagcttc accacttggt tgtgtgaatg catcaatgtg
     4561 ctctgtttgt aaatggctag ctatataatt taggattcga ggatcgtcat cgacaacaag
     4621 acattgcacc ataactataa actcccttat ctttttcatt tattatacat gtaaaatatt
     4681 tttgcgtaaa aaaacaattg ttcatattga gttcatattt caaccttata ctgacgctaa
     4741 agaagaaata gggagaagtg aatcgatatg aaattagcga taaaagagat tatgttttac
     4801 aaatttcgtt atattttaat cacattaatc attcttttat taagtattat ggtgttattt
     4861 attagtggtt tagctcaagg gcttggtagg gagaatattt cgttatttga acactttgat
     4921 aatgatgaat atgttgttca aaaaatgaaa gagccgcaaa ttgagaaatc gcaacttagt
     4981 gatacgcaac agaatcaaat taaaaaggtt attcatcaag aatcatataa aatgaatatt
     5041 caaacattga aattatctaa taaagaacaa gatgtgatta caatgaatga cgttaaacaa
     5101 caacgcatac aacttaaaaa aggtgattat cctaaaaatg ctcatgaggt cgctattaat
     5161 gataagttag ctgcagacaa cattagagtc ggagatagat tacattttaa aaataattca
     5221 actagttata gagtttctgg tattttaaac gacacaatgt attcgcatag ttccattgtg
     5281 ctattgaacg ataacggatt taatgcattg aataaggtta atacggcatt ttatccagtg
     5341 aaaaatttaa cgcaacaaca acgtgatgag cttaataaaa taaatgacgt tcaagttgtg
     5401 aatgaaaaag atttaacaga taatattgcg agttatcaag cagagcaagc accgttaaat
     5461 atgatgattg tgagtttgtt tgctattaca gcaattgttc taagtgcatt tttctatgtt
     5521 atgacgattc aaaaaatatc acaaattggc attttgaaag caattggtat taagacaaga
     5581 catttattga gtgcgttagt tttacaaatt ttaacactaa caataattgg ggtaggtatt
     5641 gctgtgatca tcatagtagg actatcattt atgatgcctg taacgatgcc tttttattta
     5701 acaacgcaaa atattttatt gatggtgggg atatttatat tagtagcgat tttaggtgcc
     5761 tcactatcat ttatcaaatt atttaaggtg gatcctatcg aagcaattgg aggtgcagaa
     5821 taatggcatt agttgttaaa gatatcgtca aaaatttcgg agaaggtttg tctgaaacaa
     5881 aagttttaaa aggtattaat tttgaagtgg aacaagggga atttgtcatt ttaaatggtg
     5941 cctctggttc tgggaaaaca acattgctaa cgatattagg cggattgtta agtcaaacga
     6001 gtggtacagt gctttacaat gatgcaccat tgtttgataa acagcatcgt tctagtgatt
     6061 tacgattgga agatattggt tttatttttc aatcttcaca tttagttcct tatttaaaag
     6121 tgatagagca attgacactc gtaggccaag aagcgggaat gaccaaacaa caaagttcaa
     6181 aaagagcaat acaacttttg aaaaatattg gtttagaaga tcgcttgaat gtatatccgc
     6241 atcagttatc tggcggtgaa aagcaacgtg ttgcgattat gagagcattt atgaataatc
     6301 cgaaaatcat tttagcagat gagcctacag caagtttaga tgccgataga gcaacaaaag
     6361 ttgttgagat gatacgtcaa caaattaaag aacaacaaat gattggtatt atgattacac
     6421 acgatcgaag attatttgaa tatgcagatc gagtgattga attagaagat ggcaaaataa
     6481 ctgattagtg gcttgtaaag acgctaaatg ttaataattt aagacatagt agtataaaag
     6541 ttagataaca gaatacgatt tgggtttaca aaaagcacgt cactattata taaaatgatg
     6601 acgtgcttta gtttatttat tttcaatata acaattgtct aatcaaggat taacgaatat
     6661 ttaaagatag tttgacgcaa tattagaaac aacctataat aatagtttat ttgtgggtta
     6721 actattataa ataaaagcag cgtatagaca tataaaccaa ctacttgaac aatataacgt
     6781 taataacaat ctatactgat acattacgcc tagataatct ttaatgagca catgtaagaa
     6841 aaagtgatat ggtgtatggc ttccgacacc atcgatagat aaacctaatt tttgggctag
     6901 tcgtaaggcg cgtaatacat gaaactgact tgttacacaa acaattttaa ctgcttcatg
     6961 atacaaattg ttgatgattt gtttagaata taaaaagttt gtgtatgtat ttatagagtg
     7021 agattccatt agtatatctg ttttatcaac accatgtgca atcaaataac gttgcatagc
     7081 taaagcttca ggaattggtt cgtctggtcc ttgtccgcca gatacaatga tctttgttgc
     7141 tgatgcttgt tgttgatata tatcaagtgc acggtctaaa cgcgctgcaa gcattggtgt
     7201 gacaaattcg gtaaaaatac cagcacctaa cacaattatg atatcaactt ctttgttgta
     7261 tgatctattt ctatatgata ctgtccaaac gagataacaa ataaaggtta gtaacaggga
     7321 aagacataat atagctaacc acatagacaa acctttcaca ataggtgact gaatcgtact
     7381 tataaataga agtgctgatg tgtagagtac aaatttatat gaaaaagata ataatttttt
     7441 aataaataag cgactagaag tatgagaaaa taaatatcta tgtttgaata gcatgataat
     7501 actgattatt ataaatgtaa caaacataga ccaagggaaa gtataggtca tgatgctata
     7561 gatgagtgac aaaaatatcg atatgacaac taagatgtag catgttaaat ttaacgtcag
     7621 agtatagttg agaattaacg gacaaataac gataagtata aatattaata ataaattcaa
     7681 taacatactg acacctcgct tataataaat attaagtata aatgtagatg atttaattta
     7741 ttaaagcaag gagaacgtag caacatgtaa gtcttaattt gttatatttt atatgggtca
     7801 atatttttgt gttttttaga tgtgcgatac aatgattgct gaggatactt tataaaaata
     7861 gtaattaggg agaaataatt atggtaaaac atttacaaga ccatattcaa tttttagagc
     7921 agtttataaa taacgttaac gcattaactg caaaaatgtt gaaagattta caaaatgaat
     7981 atgaaatttc attagagcag tctaacgtat taggtatgtt aaataaagaa cctttgacaa
     8041 ttagtgaaat cacgcaaaga caaggtgtaa ataaggccgc agtaagccga cgaattaaaa
     8101 agttaatcga tgctaaatta gttaagttag ataaaccaaa tttaaatatt gatcaacgtt
     8161 tgaaattcat aaccttaact gacaaaggta gagcatattt gaaagaacgt aatgcgatta
     8221 tgacagatat tgcgcaagat attactaatg atttaaattc tgaagatatt gaaaatgtaa
     8281 ggcaagtatt agaagtcatc aatcatcgca ttaaaacata tagcaatcat aaataggttt
     8341 ttgaaggtgt tcaaatataa gactaaatat gtatgtattg aacaccttct ttttagttat
     8401 aattgagaga gtactttcat aaataatgta ataaggtaca attctaaagt aaagttaatc
     8461 atttcttgaa ttgtattctt gggataatat ttgtaaaata ataatggtaa aataataaaa
     8521 taatttttat gatatacata taacaattga agaggaacag aattgaggtg aaagtatgaa
     8581 atcttgcccg aagtgcggac aacaagcaca agatgatgta cagatatgta cacaatgtgg
     8641 acataaattt gatagtcgtc aagcattata tagaaagtca accgatgaag atatacaaac
     8701 taataacatc aagatgagaa aaatggtgcc ttgggcaatt ggttttttca ttttaatctt
     8761 aattattata ttgttctttt tactaagaaa cttcaattca cctgaagcgc aaactaaaat
     8821 attagtgaat gcgattgaga ataatgataa acaaaaagta gcaacattat taagtactaa
     8881 agataataaa gtagattctg aagaagcgaa agtatacatt aactatatca aagatgaagt
     8941 tgggcttaag caatttgtca gcgaccttaa aaatacggta cataaattga ataagagtaa
     9001 gaccagtgta gcttcttata ttcaaaccag atctggtcaa aatatattac gtgtaagtaa
     9061 aaatggcacg cgttatatct ttttcgataa tatgagcttt actgcaccta ccaagcaacc
     9121 aattgttaaa ccgaaagaaa aaacaaaata tgagtttaaa tctggtggta agaaaaagat
     9181 ggttatagct gaagcaaata aagtgacgcc aataggtaat tttataccgg ggacatatag
     9241 aattccagct atgaaatcaa ctgagaacgg tgattttgca ggccatttaa aatttgattt
     9301 tagacaaagt aattctgaaa cggtagatgt tactgaagat tttgaagaag caaatataac
     9361 tgtaacttta aaaggcgata caaaattaaa tgatagttct aaaaaagtaa ctataaatga
     9421 ccgtgaaatg gcattttcaa gttccaaaac gtatggtcca tatccacaaa ataaagatat
     9481 taccatttca gcttcaggta aagcgaaagg taaaacattt acaacacaga cgaagacgat
     9541 taaagccagc gatttaaaat acaatacaga gataactttg aattttgaca gtgaagatat
     9601 cgaagactat gttgaaaaga aagaaaaaga agaaaacagc ttgaagaaca aattgataga
     9661 attctttgct ggatattctt tagcgaataa tgctgcgttt aatcagtcgg attttgattt
     9721 tgtatcgtca tatataaaaa aaggatcatc tttttatgat gatgtaaaga agcgtgtatc
     9781 taaaggaagt ttaatgatga ttagttcacc acaaattata gatgccgaaa aacatggtga
     9841 taagattact gcaaccgtaa gattagtaaa tgaaaacggt aagcaagtag ataaagaata
     9901 tgagcttgaa caaggtccgc aagaccgctt gcaattaatc aagacatcag aaaaatagag
     9961 tataaaactt aaataagatg catgtgtcat ggaatacaaa caacatcctt tcaaagtatt
    10021 tacaattagt gtgaattact ttgaagggat tgttttttgt gaaaaaatta tggagtgctg
    10081 aggtttatat cattttatac cttttataag ttgtaatgat gttcatgaaa cttgtgagta
    10141 atgctaatat ataaaagagc atatttaaaa ttagtttctg ttcgtgatag ttataaacta
    10201 atcataatat aaaggagata gtcatatgaa tgatttaacg cattttaaca agcgttcacc
    10261 gatatttatt attattttag gatctttgac tgccattgga gcattatcaa tagatatgtt
    10321 tttgcctgga ttaccagata ttagacatga ttttcaaaca acgacgtcta acgcacaatt
    10381 aacattatcg atgtttatga ttggtttagc gttcggtaat ttgtttgctg gtccaatttc
    10441 agattcaact gggcgcagaa agccgcttat tatagctatg attattttta cattagcaag
    10501 tttaggtatt gtttttgtac ataatatttg gctgatggtt gcattaagat ttcttcaagg
    10561 agtgacaggt ggagcagcag cagttatttc aagagctata gcaagtgata tgtatagtgg
    10621 taatgaacta actaagttta tggcattatt gatgcttgtt aatggtattg caccagttgt
    10681 tgcgccaaca atagggggca taattttaaa ttattctgtc tggcgtatgg tctttgtcat
    10741 tctgactatc tttggcttcg tcatggtcat aggttcttta ttaaaagtac ctgaatcatt
    10801 aacagtgaca aatcgtgagt caagtagtgg attaaagaca atgtttaaaa acttcaaaat
    10861 attattgaag acgccacgtt ttgtattacc aatgctcatt caaggtatga catttgtaat
    10921 attatttact tatatttctg cgtctccgtt tattattcaa aaaatttatg gcatgactgc
    10981 aatacaattt agttggatgt ttgcaggaat tggtattacg ctcattatat caagtcaact
    11041 tactgggtac cttgttgatt ttatagactc acaaaaattg atgagaggca tgacgatgat
    11101 tcagattatc ggtgtaattt tagtgacgat tgtattgctt aatcattgga acttttggat
    11161 tttagcgata ggatttatca ttttaatagc accagtgaca ggtgttgcga cacttggttt
    11221 tacaatagct atggatgaaa gtagtagtgg tagagggagt tcatcaagtt tattaggttt
    11281 agtgcagttt ttatttggtg gtattgcatc gccattagtg ggggtaaaag gtgaagataa
    11341 tcctatacct tacataatta ttattatagc tacagcagtt atattaatta ttttacagat
    11401 atataatatg aaggtattta aaacaaatcg atgataaaca tagtttcttc tattataatg
    11461 aattgaaaaa tttaaaaaca aaacttgagc tattcataga cctatgaatt tagcatcaaa
    11521 aataaaaa
//
LOCUS       DAMZPP010000094        11450 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMZPP010000094 DAMZPP010000000
VERSION     DAMZPP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11450
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.094"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            13..120
                     /locus_tag="POH73_001916"
     CDS             13..120
                     /locus_tag="POH73_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887852.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            complement(173..349)
                     /locus_tag="POH73_001917"
     CDS             complement(173..349)
                     /locus_tag="POH73_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDI6887853.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(499..795)
                     /locus_tag="POH73_001918"
     CDS             complement(499..795)
                     /locus_tag="POH73_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HDI6887854.1"
                     /translation="MGWFKKHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIKL
                     DKTLDAIQKEREIDEKNKKENDKNIRDMKMWVLGLVGTIFGSLIIALLRMLMGI"
     gene            complement(853..1140)
                     /locus_tag="POH73_001919"
     CDS             complement(853..1140)
                     /locus_tag="POH73_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887855.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVERSDLSPEVTVNTETV"
     gene            complement(1187..1339)
                     /locus_tag="POH73_001920"
     CDS             complement(1187..1339)
                     /locus_tag="POH73_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887856.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            complement(1329..5114)
                     /locus_tag="POH73_001921"
     CDS             complement(1329..5114)
                     /locus_tag="POH73_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887857.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDTLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTKTLFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDDLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGDNNRYVQIQNDSIELGGIVQRTWRGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG"
     gene            complement(5130..6614)
                     /locus_tag="POH73_001922"
     CDS             complement(5130..6614)
                     /locus_tag="POH73_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDI6887858.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGIKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFTIPVKFTIKVVLTDPYKYSVTGNKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFPDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            complement(6611..11140)
                     /locus_tag="POH73_001923"
     CDS             complement(6611..11140)
                     /locus_tag="POH73_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDI6887859.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTHSYKQRIKELDGTITGYKKNVDDLAKQYGKVSQEQGENSAEAQKLRQEYNK
                     QANELNFLEKELEKTTTEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGLMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGGELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTGKELENATESFLKFSHITGSEGVQAVQLITRAMG
                     DAGIEASEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKNPREEFKKTLAEIEKTPDIASATSLAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSIAVDWFSNLSDGSKRSIVIFSGIAAAIGPVVFGLGAF
                     ISTIGNAVTVLAPLLASIAKAGGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNGAIESVKQTFSNFIQFIQPFVDSVKNIFKQAISAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKAIFEFILNFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGALNIILGLIKFFSSLFVGDWRGVWDAVVMILKGAVQLIWNLV
                     QLWFVGKILGVVRYFGGLLKGLIAGIWDVIRSIFSKSLSAIWNATKSIFGFLFNSVKS
                     IFTNMKNWLSNTWSSIRTNTIGKAQSLFSGVKSKFTNLWNATKEIFSNLRNWMSNIWN
                     SIKDNTVGIASRLWSKVRGIFTNMRDGLSSIIDKIKSHIGGMVSAIKKGLNKLIDGLN
                     WVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEMI
                     EFPNGKRVITPNTDTTAYLPKGSKVYNGAQTYSMLNGTLPRFSLGTMWKDIKSGASSA
                     FNWTKDKIGKGTKWLGDKVGDVLDFMENPGKLLNYILEAFGIDFNSLTKGMGIAGDIT
                     KAAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAAYTAATGRPFHEG
                     VDFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTM
                     VKPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGRLSIGGGGATSGS
                     GATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSR
                     GLFQIIGSTFRANAKRGYTNFNNPVHQGISAMQYIVRRYGWDGFKRAGDYAYATGGKV
                     FDGWYNLGEDGHPEWVIPTDPARRNDAMKMLHYAAAEVRGKNASKNKRPSQLSSVNGF
                     DDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQ
                     ESTKVKFRKGGIAIQ"
BASE COUNT         3288 a         2408 c         1610 g         4144 t
ORIGIN      
        1 taagccgtct atttgatatt tatattatgg tgtgttaatt tatatataga aaaagggcaa
       61 catgcgcaaa catgttaccc taatgagccc gttaaaaaga cggtggctat tttagattaa
      121 agattaaatt aataaccatt taaccatcga aaccagccaa agttagcgat ggttattttt
      181 tattgcttaa ttcaataagc ttgattacta gacctatcaa tgcaataagg aataaaccaa
      241 actgcaacat ggtactaatt gtaatcatta ggcgtctcct ttctaaagat ttcagtaatg
      301 ccaccatagg caccacctcc ttatactcag atagccacca tctatccaac ttgctcactt
      361 ctgcatatta ccataattac aacaataaat aaaaagtcag taccgaagca ctgactaaaa
      421 cttatttaca tttaccgaac caaaaacatg tccagaaact ataaccaaag attagtttaa
      481 acattttatt cacctctctt atatgcccat aagcatacgc aataatgcta taattagcga
      541 cccaaatatt gtcccaacta aaccaagcac ccacattttc atatcacgta tgttcttatc
      601 attttctttc ttattctttt catctatttc tctttctttt tgaatagcat ctaaggtttt
      661 atctaattta atgttaactt gctcttgggt tttttgacct aatttaattt cgttgagtgt
      721 gctgagcatt gttttatcat tctcttctaa ccttctgatg cgccattcat gttcgtgttt
      781 tttgaaccac cccaattcag tacacccgct ttctaaaaga ataaagacta tgagtatcta
      841 actcatagct tttcatactg tttcagtgtt aactgttacc tctggagata aatctgatct
      901 ttcaactact tctttaacta ctttcacacg ttgttttttg ttagttaatt gatataacaa
      961 atttaacgtc tccgcaattt tcttagcgtt ttcttcagat ttaaaatctt gagcatggtt
     1021 aaccatttca gaagttgtaa aacttcctgt gaaatcttga tatactacac gttctgtacc
     1081 ttctttgtcg atttgtacta aaataaacct ttctgtattg ttgataattt cttttgccat
     1141 aattaaatga cctccttaaa tttttgtata aaaatagtgc taaggattac tcttcctcag
     1201 cacattgttg attttcttta ttttcttgta tatacgcttt taacatcgcg ttttcttgtg
     1261 ttaacctcat aatttcctgt gataaataat gaattgtata ttcaggatta gcttgtaatc
     1321 cttgtttgtt atcctgcatt ctttgactcc tccaatttct tgattcttag ttgttgttct
     1381 ttgataacag ggataagatg aatccataga cgatcatacg ctataccttc aatttctcct
     1441 ttgtcatcat acgtgacaaa ctcttttaat cctaaattct ccacctcttc agcaatcaaa
     1501 cctacgtatc tatcaagttt ataggtgtct tccgataatt ttctatcttc tctcagctct
     1561 ctagctaaaa tttcagactc agctttatca aaccacgttc taataggtaa gttaagaata
     1621 gcttttgaat gttccagttg ttcatctcta tcgttatatt gattttcgat agataacttg
     1681 tatttacgcg ctgatgtcga acgcccaatt gtgccagcag aagtaatatg caaattagct
     1741 gcggccgaat aagtacgtct ataaattgag ttagaagcta tcctatctcc tgcatcatct
     1801 gaacctacag acagtaggtc tgtactctgt atatgaatat acctattacc atcacgtcgt
     1861 ttcagcatat taaatttgcc ataccctgct tcgattgttg tatctccacc tgttgcatat
     1921 cgtccattaa caatttgaac aagaccttta tttctttctt tagaaaacct gatacccgca
     1981 ccgtaatcat agttctcatc agaaccaaac ataatataac cgtcactcga ataagcatta
     2041 tctgcattag acagcgtgaa tgcaaatcgg tttaatccag gcactttgtc tgtgtttgga
     2101 tataaataca ccggtgcctg tttgcttttg atattcgatg aagcgtaaga ctccagaaca
     2161 acccgattat tatctgacgt tagtgcaacg acaccaccat aggaattgat tgttatacca
     2221 ttcatgccac tatcactgta agttttatcc caccattgaa tcgtaccaga tgaaccaccg
     2281 tcttcacctt caccatcaat ataagtcgaa ataccaaaat gtgacatata aagtgaaccg
     2341 ccagcggtgt tatttctaaa tcttaggtga ccgtctttca gtcgcgtaaa aatatcgtct
     2401 gttgaacgtt tccctctcca agtacgttgc acaataccac ctagttcaat agaatcattc
     2461 tgtatttgaa catatctgtt attgtcaccg cctttaattc caattctatt aacattgata
     2521 tcaagaccct ctcttgataa attaaggctg ttgacaatat cggttttatc tactttatct
     2581 cgcatatttt ggataagaag gtttatttct ctattaccgt taatatcaat tttatcagca
     2641 tttaatctaa taccacgtgg ccccacattc aaagcttgag ccactccgtt atcatcatat
     2701 ctgattgttg ttccatctgt aacgttttgg acaatctcgt ttaatatatt tgaaagtgta
     2761 cgattggttg cattaaactc ttctttagta gttcttaatt tgatttcctt accattttgt
     2821 ataatttgag aaccatagcg agtcagtgtt ctcctctgtg catctgtgct ttctttgacc
     2881 ttgttgtctg tataagcatt agctttcttt tcagcgtttc tagcctttag ttctgcgttt
     2941 tgttttgcct cttcaagttt agcttgagca tcttgtatag cgcgttgctc ttcttccgaa
     3001 attttaccat cagcatacgc ttgcgattcc ttctctttaa gatcatcttg agcatcaatg
     3061 tatgatttta aagcttcttg cgcttcttga tttgcttgtt caatacttgc tttaatctca
     3121 ggattattgg acaaatcact taactggtca tcagtatatt gtttttgttc ttccaatccg
     3181 tttcgatatt cgtttaacgt aactttatct ttgatttcac cttttaaagt cgttctctca
     3241 gcttcagcag tatctaaacg ttcaacaata ccgtctttgt ctgttttata gtccgatgtt
     3301 tttacatagt cacgtaattg ttcttttgtg gattctctag ctgcttcaat agctgattta
     3361 acaacattag gttctccgac taactgcaaa tcttcattca ccgttaaacc aaattttgtt
     3421 gctattattt ccaacgcttc tttatatttt tcatcagtgt attgtgactg taataattta
     3481 aatctatctg aaatggcgat tttgacatct tctacatctg tataaacatc ttgtaatttc
     3541 tttctatact caagaaataa agtttttgta tctaccaacc gaccaatcgt tgcagtttcg
     3601 ggtgtcatag attctaaatt atttttaatt tgattataaa catcaatcac agcgtctaaa
     3661 cttgcttgta agtccgcttt caaatcatta tctactaagt actcgctatt cagtaattct
     3721 gtagcttctg acaaaagact agcgtgttgt atagataaat taataaaaat attgtttaat
     3781 tcactgaata gcgctttctc tcttgttata ccacctaatt tttcaacatc atttggtgtt
     3841 gcttcaatcc atcgaccatt ccaatatcta cgcaagacag caacatcagg gttacttgta
     3901 tcataccaaa gcgtatcatt gactggattt tctggcggtg tatcactttt atgaattttg
     3961 cgttcaaagt attctaattc accatctaca acatctttta ctatagtatt gatattgcta
     4021 atattgtcgt ttaatttttg gtgtattagg tttaatcgct tgttaaactc ttctcgtaat
     4081 tctgattctt tgaactcttt aggttgaccg aatgtatatg tgctattttc tgaaattatg
     4141 ttatattctt cagcaataac ttctgcctct acatacaatg gcgggttaaa atctctatgt
     4201 tttactctga ctgtatcgcc aattgatata atctcgtgcg gatacgtaac ttccaaatca
     4261 gtagaagtaa tctcatatga cataactgcc gacttacgtt tatttaactc tgttttggct
     4321 aaagaactta atcgtgtttc attcatattt tgatcatctg attgtggttc atatatcccc
     4381 caaatatagc gcataggtag gttgaattga ctttgcgctt cgtcatctgt cacaactagc
     4441 tctaaacgct tccctttgtc attttcaggt cccacagcaa ttaatgctgt tttgatttct
     4501 gacatatcaa tcttcctagt taacccgact aaatctttac catattcaat ttctttacct
     4561 ttgaataagc tgtttttctt tttgagtact acatatctac ctttgacggt attagagcta
     4621 agctcaatat aaaaatctaa aaccatttta taggttgtac ataattgctt taaaacttca
     4681 tatctagttt gataagaagt ccatgacgta gtacgtaagc catcgtattc ggtttgttca
     4741 gaaacttccc aacctgtatc gctcaacaca tctttcaatg cttctgaagt tgtctttttc
     4801 tcaaattttc ctggtgcata cggtttagct gttgttatat cagcaagata agacgctata
     4861 cattctatct ctgtgtagcc gtccatcgta tcttgaaccc agttaataat aaattcacgc
     4921 cattgtttgt ttgaatccct tataataaca cgatgtcgtt cacggaactt ttcagctctt
     4981 tctgatgata tgagcagttc aagcatttct gaattgtcat taacattacg tttatgaatc
     5041 gctctaacta aggaagggtc atcagtagaa aggaaatcta taatcttgtc gttaaaatct
     5101 aaaacatgta tcacactctc atctcctttc tataaatatc tatcttgcca tttaaccgtc
     5161 gtatcaaaga cgttttcagg ttgtatgatt aattcactgt acccagaatc aacattgaaa
     5221 taattacttc caaacgattt ctcgctcaac attggttcct cattgatgac aacacttttt
     5281 gcttgcatat ctattttcac taaatcacct ttttgtataa tgacatccct tgcgcctttc
     5341 ggtttcggta gaatctccgt attgaatgaa cctaatccat tcatctccat ccacttataa
     5401 ccgttatact tcgcactata gatagctatg atagaagctg gacgctgata aaacttaccg
     5461 ccatctatcc actctttctc atccatatca ataggtttac gtctatctgg gtctttaatg
     5521 tgatcaaatt tccaagtttt aatagaaaat ttattaccta ctcttctgag ccgcatataa
     5581 acaacgattc tgtccaagtt atacattatc ggtttattct gatagtcgta tatctttttg
     5641 gggtctcctt tttggttata caacgtaaca acaatatgtc ctatttttct atcatgatat
     5701 ttattttcat aaccaataga agcaagtaac ttaccatcac tatcataaat atgttgtgct
     5761 gttcttccgg cacctttacc tttttgttca acaatacatt tataggtaat ttgaaaatct
     5821 gtcatcgctt tagggagccc tcgtttcgtg ccagcaccaa cccaaccttt tgcatcagga
     5881 aaattagttg ctttatatcc ttcgccaaga ttggatatca caaagtcacc gccgacctta
     5941 ccacctaagt cattacttgg aatatcttca gtaatcatct tagtccaacc tttgaaatca
     6001 cgaaactcac tatgataaac aggaggcatg taatccttaa cttctttggt tacctcatca
     6061 tcaccaacca taaaataatc ttcatcattt ttagtaatca taaagtaact agatggttta
     6121 attgctcggg cttcaacaat taaaggagtg tcagcagtcc cactatttac aactgaaact
     6181 tggtctgaaa tcgcagtatt tttatttcct gttactgaat atttgtaagg gtctgttagt
     6241 actactttga tagtgaactt aacaggtatt gtaaattctt tgtgcagctt tattggtcct
     6301 tcgaaataag cgttccagta ccaatcttta gatttgaatt gtaatttaac ttgttcctcg
     6361 tagttaaaaa actttactaa ttcattcaag acgtcatcat gtgttttaat gccgttgtga
     6421 gataaatagt cattacgtac caccaaaggt atatcaaaac tataagattc aagcctacgc
     6481 cctttatata tagaccccga acgtccatct acattttctg tttttaaaac ataattaaaa
     6541 gagggtattt caaaccctct ttcgacatac aaccaaggaa ttgttttgtt gttcacttta
     6601 atagtgtcta tcattgaata gcaattcctc cttttctaaa ctttactttt gttgattctt
     6661 gcctttctcg cttttctata gacgcgttca cctttttatc aaaagcgtat tcgtcaataa
     6721 tcggctgata atctttatct gcaatcacat cgttagattg cgctatcttc agtaataaag
     6781 ctatttgttg ttgctgttgt tcaatcattt tcaataataa gcttgggtca tcaaacccat
     6841 ttacactaga caattgacta ggtcgtttat tcttacttgc gtttttccct ctaacttccg
     6901 cagctgcata atgtaacatc ttcattgcat catttctacg agctggatct gttggaataa
     6961 cccattctgg atgaccgtct tcacctaagt tataccaacc atcaaaaact tttccgcctg
     7021 tagcatatgc gtaatcaccc gcacgcttaa atccatccca accgtagcgt ctaacaatgt
     7081 actgcattgc tgaaatacct tgatgaaccg gattattaaa gttagtatat ccacgtttag
     7141 cgtttgctct aaaagttgag ccgatgattt ggaataatcc tcttgatggg tctcctcttt
     7201 gagcatttat atcccagtta ttcactgcat ttgactggta gttactttca cgttttgcaa
     7261 cgcgcatcat ttggtcatga atccatttac ctttataacg accacctaaa atactttgcg
     7321 cttgtcggat tactcgactg gcataagttg cgccacttcc agaagtagca ccaccaccac
     7381 ctattgataa tcttcctttt ttcttagcat tccttaaata tggttcaggg tcaaaatggc
     7441 gtccgtttct cctcatttca aaatgtaagt gtggtcctgt actaaatccg gtattaccag
     7501 ttaaaccaac aacatcaccg ggctttacca tcgtgccact aggtggtgat ttgctaaagt
     7561 ttttcaaatg cgcaaatagc atatcgataa cgccactagt aatttttaca taattaccat
     7621 aaccaccaga cataaatggc attcttgtaa gtctgccacc catcggcgtt ctaacttctt
     7681 gatatacaaa tggaaaatcg acaccttcat gaaatggtct tccagttgca gcggtataag
     7741 ctgcggtacg tccataatga taattaattt tgtcagggtc taatattccg ccgactaaat
     7801 cgccaccgcc catagcttct aaattttctt ttatccaatc agtagcactt ttcttaatct
     7861 tagaccatgc agcttttgtt atgtcgcctg caattcccat acctttagtt aaagaattga
     7921 aatcaattcc aaaagcttca agtatataat ttaaaagttt gcctggattt tccataaaat
     7981 ctaaaacatc gccaacttta tcgccaagcc atttggtacc tttacctatt ttatcttttg
     8041 tccagttaaa tgccgatgat gcaccagatt taatatcttt ccacatagta cctaaactaa
     8101 atcttggaag cgttccgttt aacattgaat aagtttgtgc accgttgtat acttttgagc
     8161 ctttaggtaa ataagcggta gtatctgtat taggtgtgat tacacgttta ccgttaggga
     8221 attcaatcat ttcatttcta aaaccatttg gaccatttcc gcgtccctta tccccaactg
     8281 tagcgaatgt gtcacgtgca atcttaccgt tcttaactaa tcttgtagta gtatgtgtgt
     8341 gctctgtacc agtgtgtaac ttaggtattt tatccattcc caacttacca ccgacccagt
     8401 ttaaaccgtc gattaattta ttaagtcctt ttttaatagc gcttaccata ccgccgatat
     8461 gacttttaat cttatctata atggaactca agccatcgcg catatttgtg aaaattccac
     8521 gtaccttact ccataaacgg cttgcaattc ctaccgtatt atctttaatg gaattccaaa
     8581 tatttgacat ccaatttctt aaattactaa aaatttcttt cgtcgcattc cataaattag
     8641 taaattttga tttgacgcca ctaaataatg actgcgcttt tcctattgta ttcgtacgga
     8701 tactgctcca agtattagat aaccaatttt tcatatttgt gaaaattgat tttacgctat
     8761 taaataaaaa tccaaaaata ctttttgttg cattccaaat tgctgataaa gatttactga
     8821 atatacttct tattacgtcc caaattcctg ctatcaatcc ttttagcaac ccgccaaagt
     8881 acctaacaac accaagtatt ttacctacaa accataattg aactaaattc caaattaatt
     8941 gaactgctcc tttaagaatc atcacaacgg cgtcccaaac tcctcgccaa tcaccaacga
     9001 ataaacttga gaagaactta atcaagccaa gtatgatatt taaagcacct tgtattacac
     9061 cttttatgtt ctcccaagta ctgacaatca aggctttaac cgccggccaa ataaattgca
     9121 tcacttgcca aatcgcgaac ataattggtt taattacaaa atttaaaata aattcaaata
     9181 tcgctttaat aaagttgcat atattttgaa gtgcttgaac aatggaaatt ccgttttcat
     9241 taaagaatcc attgatttga ctccaaatat cttttgcgaa atcaactatt gctgatatcg
     9301 cttgtttaaa gatgttttta acagaatcaa cgaaaggttg aataaattga ataaaattac
     9361 taaatgtttg tttaacactt tcaattgcac cattaacaaa atttctaaat gtttcagatt
     9421 tcttataagc aattgtaaat gcgactgcta aaccagccaa tacacctaat acaatgccaa
     9481 ttggaccagt taaagctgtg aagacagttc ctaatatagg tactttagtc gataaaaaac
     9541 taatcaatcc accagccttt gcaatactag ctaacaatgg agctaataca gttactgcat
     9601 tgccaattgt acttataaat gcacctaatc caaaaactac aggaccaatt gcagcagcaa
     9661 taccactgaa aataacaatt gatcttttag aaccatcact taaattggaa aaccaatcaa
     9721 ccgctataga tagctttttg attaattctt ccattacggg agcaaacgca ctttcaatag
     9781 aagcccatac atcagcacct actaatttta atttattcat tgctacttta aatctttcgg
     9841 agccactttc agaatcttta aatgtttggt ttactgtgcc ttgggaatct tcaatagttt
     9901 ttaaaaattc ttgataacta aagcgaccac ctttaatagc gtctgctaaa tcaggacctg
     9961 cctttgcacc aaatgcttca atcgctaaac ttgttgcgct agctatatcc ggcgtctttt
    10021 caatttctgc taatgtcttc ttaaattctt ctcttgggtt tttaccagct ttaccccaat
    10081 ttgatatagc ttttttcaaa ccactgaatg ctatttcagt attaacgcct gacttttccc
    10141 attgagagaa taaagcaatt gattctttca tctcaaagcc catagctctc attggagcgc
    10201 cgtatttagt aatactatca gctaatgtat caacacttat cccactagct tgcgccgctt
    10261 ttgctaccat atccaaaaca ctttgatatt cacttgcttc gatacctgca tcgcccattg
    10321 cacgggtaat taattgaacg gcttgtacgc cttcagaacc tgttatatga ctgaatttca
    10381 agaatgactc tgtggcattt tcaagttctt tacctgtaaa acctaacctt gtattaactt
    10441 ctcctaaaac accgcctaca gtctcagcat ctgctggaaa gttgccataa acatctttaa
    10501 atgaattctg caacttctta agctctccgc cggttgctcc tgttgcttgg gtaactgtat
    10561 ctaaaccttt atcaacttct gcaaaagctt ttcctgatgc tgctgcaata cctaaaacag
    10621 gtgcggtaac accaatcatc aaacctttac caatggattt taaaccatca cccatttttg
    10681 ttaatttagg tcccatactt tcaaaaactt tactggtttt tccccagcca ctttctgcca
    10741 ttctttgagc ttcaacttga gcttttttga actcttcaaa ctcagttgtt gttttttcta
    10801 gttctttttc taaaaaattc agctcatttg cttgtttgtt atattcttgt cgtaattttt
    10861 gagcttccgc gctgttttcg ccctgttctt gagatacctt gccatattgc ttggctaaat
    10921 catcaacgtt tttcttataa cctgtgatag ttccatcaag ttctttaatc ctttgtttgt
    10981 aactatgagt tgatttttcg gtatatttga agttgttacc ggttaacttt aagtcagaat
    11041 ttaaagtttt aaagtttcgt ttgatttctg caaatgatct atttaaattt gctgcatcta
    11101 aatccaaacc tatagataaa ccttttattc tttctcccat tttttacctc ctttctaaaa
    11161 aagttcaaaa aaataaccct aaccaaacgg ttaaggttaa aacgcatcaa ttaaagcctc
    11221 tgctttttct tcagaaatgt cattgttttt attttgatat atggaaagta cataatgaaa
    11281 tggcattttt aaaacttcgt tagcgtcttt accattttca attaagtcca tcatgagagt
    11341 atccatattt ttcaacattg ctttatatgt taaatcttca ggctttattt catgttctgg
    11401 ataaaatttc tagtttcctc agtttgctga ccttgagtaa tgaaaattac
//
LOCUS       DAMZPP010000095        11433 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMZPP010000095 DAMZPP010000000
VERSION     DAMZPP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11433
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.095"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(15..617)
                     /gene="rpsD"
                     /locus_tag="POH73_001924"
     CDS             complement(15..617)
                     /gene="rpsD"
                     /locus_tag="POH73_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDI6887860.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPSQTISVREKSQKLNVIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(861..1325)
                     /locus_tag="POH73_001925"
     CDS             complement(861..1325)
                     /locus_tag="POH73_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDI6887861.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQVVADVHQFKGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            1462..3156
                     /gene="ezrA"
                     /locus_tag="POH73_001926"
     CDS             1462..3156
                     /gene="ezrA"
                     /locus_tag="POH73_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDI6887862.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQCEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(3230..3365)
                     /locus_tag="POH73_001927"
                     /pseudo
     CDS             complement(3230..3365)
                     /locus_tag="POH73_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792848.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3515..4663
                     /locus_tag="POH73_001928"
     CDS             3515..4663
                     /locus_tag="POH73_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDI6887863.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     DQLLQQAKVQINDMVNTNMTYDVVFTSGATESNNIALKGVAYRKFDTAREIITSVLEH
                     PSVLEVVRYLEEREGFKVKYVDVTKDGTIDLQHFNDLMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAEVIKNYPKAHFHVDAVQAFGKISMDFNNVDSISLSGHKFNGLKGQGVLLVNH
                     IQNIEPIIQGGGQEYGVRSGTVNLPNDIAMVKAMKMANENLEALNAFVTELNNDVRQF
                     LSQYQGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            4663..5886
                     /gene="thiI"
                     /locus_tag="POH73_001929"
     CDS             4663..5886
                     /gene="thiI"
                     /locus_tag="POH73_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDI6887864.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITNRLSKIFGIKSISPVLKVEKSIEAMSAATIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGAYAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            5930..6700
                     /locus_tag="POH73_001930"
     CDS             5930..6700
                     /locus_tag="POH73_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDI6887865.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSAIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAILFAALFILIGFYDGFVGGGTGS
                     FMLFVLLIFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLVMAASMIVGSY
                     AGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            6798..7292
                     /gene="tpx"
                     /locus_tag="POH73_001931"
     CDS             6798..7292
                     /gene="tpx"
                     /locus_tag="POH73_001931"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDI6887866.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSEASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            7415..8362
                     /locus_tag="POH73_001932"
     CDS             7415..8362
                     /locus_tag="POH73_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6887867.1"
                     /translation="MAEQQTIMERLFHSLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGTGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSRDFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKSVEVLLANIPDFKNPSQFQGFMTELNQWMDTNRPKK"
     gene            8449..9651
                     /locus_tag="POH73_001933"
     CDS             8449..9651
                     /locus_tag="POH73_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDI6887868.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVDGEKVKTVQDIKDHVEAVDIMLDAFKKHNIINDINDIDGTGHRVVHGGEKFPESA
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLQRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTYGGLK"
     gene            complement(9898..10398)
                     /locus_tag="POH73_001934"
     CDS             complement(9898..10398)
                     /locus_tag="POH73_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDI6887869.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            10539..>11433
                     /gene="ald"
                     /locus_tag="POH73_001935"
     CDS             10539..>11433
                     /gene="ald"
                     /locus_tag="POH73_001935"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDI6887870.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAV"
BASE COUNT         4025 a         1589 c         2146 g         3673 t
ORIGIN      
        1 tgtattgaac ggtattatct tgagtagtac tcaacgatta attgttcgtt aatttcagca
       61 ggtaattcgc tacgttctgg taaacgtacg aaagtaccag ttaagctgtc agcatcaaag
      121 tttaagtact caggtacgaa attgttgatt tcaactgatt caacgatgac gtttaatttt
      181 tgagattttt cacgaactga aattgtttga ctaggtttaa cagaataaga tggaatatca
      241 acacgtttac catctactaa gatatgaccg tggttaacta attgacgtgc ttgacgacga
      301 gtacgagcta aacctaatga ataaacaaca gcgtctaaac gacttgctaa taagatcatg
      361 aagttttcac cgtgtacacc gaatttttta ccagcgatgt caaatgtgtt acggaattgt
      421 ctttcagtca ttccatataa gtaacgtaat ttttgttttt cacgtaattg taaaccatat
      481 tctgataatt ttttacgttg gtttggacca tgttgtcctg gtgcgtaagg acgtttttct
      541 aattctttac cagtaccgct taaagagata cctaaacgac gagatttttt ccagtttgaa
      601 cctctgaatc gagccataat aagactcctc ctttaattct tttttgttgt tatgaataaa
      661 caaaaaagag tgttgcatgc ttactaagat atattgtttt atgtgtcttc accttatagc
      721 gacagttacg tgacacgtcc gcattgggaa caacatagcg ctcaataata tacaactgct
      781 actttcgtta attcacacaa agtttattgt aacattttcc tgctaactgt caacacttta
      841 ttgaaaatgc tgatgtcctt ttatgcgagt tgcttttcaa taattttaac aattgcttca
      901 agatgttcct tgtcattgtc atcaaatcga tccgttatag gggcatcgat atctaagaca
      961 ccgataattt tatcatcttt aaaaattgga acgacaattt cagacttact attagcatca
     1021 caagcgatat gtcctttgaa ttgatgaaca tcagctacaa cttgtgtacg acgttctgaa
     1081 acggctgtac cacatacacc ttttccaatt ggaatgtgga cacaagcggg gtgtccttgg
     1141 aaaggtccaa gtataagttc attttgttcc aataagtaaa agccgaccca attaatttga
     1201 tctagattat catttaataa ggcagacatg ttacttaaaa tagcaatcat gtgatgttca
     1261 tcttcaataa ggcttgctgc ttgtttcttt aataatgtgt agtttgttgg gttaattgtt
     1321 gtcatattta aaacctctta ttttcataga ttttataata gtatagaatt tcgaaaatat
     1381 tatggcaata aattcatttt ataatttatc cttaagcaaa attacgttat aatagtaatg
     1441 ataaattagg aggagaagca tatggtgtta tatatcattt tggcaataat tgtgattata
     1501 ttgattgctg taggtgtatt attctattta cgttcaaata aaagacaaat aatagaaaaa
     1561 gcaatcgaac gtaaaaatga aattgaaacg ttaccttttg atcaaaacct tgcacaatta
     1621 tctaagttga atttaaaagg tgaaacaaaa acgaaatacg atgcaatgaa aaaggacaac
     1681 gtagaaagta caaataagta tctagctcct gtggaagaaa aaatccataa tgctgaggct
     1741 ttattagata aatttagttt caacgcatct caatgtgaaa ttgatgatgc aaatgagttg
     1801 atggatagtt acgaacaaag ctatcagcaa caattagaag atgtaaatga aattattgcg
     1861 ttatacaaag ataatgatga attatatgac aaatgtaagg ttgattatcg tgaaatgaaa
     1921 cgtgatgttt tagcaaatcg tcatcaattt ggtgaggcag caagtcttct tgaaactgaa
     1981 attgaaaaat tcgagccaag gttagagcaa tatgaagtac taaaagctga tggtaattat
     2041 gtacaagcgc acaaccatat agctgccttg aatgaacaaa tgaaacagct aagatcttat
     2101 atggaagaaa taccagaatt aattagagaa actcaaaaag aattacctgg tcaattccaa
     2161 gatttaaaat atggttgccg tgatcttaaa gttgaagggt atgatctgga tcacgtaaaa
     2221 gtagacagta cattacaaag cttaaaaaca gagcttagtt tcgttgaacc attaattagt
     2281 cgcttagaat tagaagaagc taatgataaa ctagctaata tcaatgataa gttagatgac
     2341 atgtatgatt taattgaaca tgaagttaaa gctaaaaatg atgtcgaaga aacaaaagat
     2401 atcattacgg ataacttatt caaagcaaaa gacatgaatt atacattgca aacagaaatt
     2461 gaatatgtac gtgaaaacta ctatataaat gaatctgatg ctcagagtgt tcgtcaattt
     2521 gaaaatgaaa ttcaaagttt aatttctgta tatgatgata ttttaaaaga aatgtctaaa
     2581 tctgctgtac gatatagcga ggttcaggat aatttacaat atttagaaga tcatgtcaca
     2641 gttattaatg acaaacaaga aaagctacaa aatcatctga ttcaattgcg tgaagatgaa
     2701 gcagaagcag aagacaatct gttacgagta caatcgaaga aagaagaagt gtatcgtcga
     2761 ttacttgctt ctaacttaac aagcgttcct gaaaggttta tcatcatgaa aaatgaaatt
     2821 gatcatgaag ttcgtgatgt taacgaacaa tttagtgaac gtccaataca cgttaaacag
     2881 ttaaaagata aagtgtctaa aattgtgatt caaatgaata catttgaaga tgaagcaaat
     2941 gatgttcttg ttaatgctgt ttatgcagag aaattaattc aatatggaaa tagatatcgt
     3001 aaggactata gcaatgttga taagagctta aatgaagctg aacgattatt taaaaataat
     3061 cgctataagc gtgcgattga aattgcagag caagctcttg aaagtgttga gccaggtgtc
     3121 actaaacata ttgaagaaga agttattaag caatagaaaa tagtatgtag ttatacttaa
     3181 ataatatgag ctctctgtca aattggactg atgagtttaa taattgaagt tagccaacga
     3241 tacgttgtct agcttctttt tttatatgga tatatgaaag ggacaataaa tataaatagc
     3301 aattgtttaa agataaacgt aatcaaatgt gaagtttaaa ttaatataag tagtgaaaaa
     3361 agcatcgtgc tatgcattga gtgaaatagt tcaattctta ttaaaatggg tggaaaaatg
     3421 attaattttt gttatagcct ttatttttta tgtgataata gtataatgct tgagtaaaaa
     3481 caattttaaa agattgatga ttaatgaaag agaagtgttt attttgatat atctagataa
     3541 tgcggcaacg acgaaagcat ttgaagaagt gttagatact tatttaaaag taaatcaatc
     3601 aatgtattat aatccgaata gtccgcataa agctggtttg caagcagatc aattattaca
     3661 gcaagcaaaa gttcaaataa atgatatggt caatactaat atgacatatg atgttgtttt
     3721 cactagtggt gcaacggaat ctaacaatat cgcgcttaaa ggtgtggctt atcgtaaatt
     3781 tgatacagca agagaaataa ttacatctgt tttagaacat ccgtctgttt tagaagttgt
     3841 cagatatttg gaagagcgcg aaggctttaa agttaaatat gttgatgtaa caaaagatgg
     3901 cacgattgat ttgcaacatt ttaacgattt aatgtccgat aaagtcggtt tagtaacatg
     3961 tatgtatgta aataatgtaa caggtcaaat tcaacccatt ccacaaatgg ctgaagttat
     4021 aaaaaattat cctaaggcac attttcatgt agatgcggtt caagcattcg gcaaaatttc
     4081 aatggatttc aataacgtag atagtattag tttaagtggc cacaaattta atgggttaaa
     4141 aggtcaaggc gtcttacttg ttaatcatat tcaaaatatt gagccaatca ttcaaggtgg
     4201 cggtcaagag tatggtgtta gaagtggtac agtcaattta cccaatgata ttgctatggt
     4261 taaagcgatg aaaatggcta atgaaaacct tgaagcattg aatgcatttg ttactgagtt
     4321 aaataatgac gtccgtcaat ttttaagcca atatcaaggt gtttatatta attcatcaac
     4381 ttcaggttca ccattcgttt taaatattag ttttcctggc gtaaaaggtg aagtattagt
     4441 taatgctttt tcaaaatatg acattatgat atctacgacg agtgcttgtt catctaaacg
     4501 taataaatta aatgaagtat tggctgcaat gggattatca gacaaatcta ttgaaggtag
     4561 tataagatta tcatttggag ctactacaac taaagaagat atagcgaggt ttaaagaaat
     4621 atttatcatc atttatgagg aaattaagga gttgctaaaa taatgaagta tgatcacttg
     4681 cttgttagat acggggagtt aacattaaag ggttcaaata gaaaaaaatt tgtaaatcaa
     4741 ttaagaaata atgtaaataa gtcattaaaa ggacttgatg gatttgtcgt taaaggcaaa
     4801 cgagatcgta tgtatattga acttgaagac catgcagata taaatgaaat aacaaatcga
     4861 ttatcaaaaa ttttcggtat taaatctatt agtccagtat taaaagtaga aaaatcaata
     4921 gaggcaatga gtgcagcgac aattaaattt gcgcagcaat ttgaagaaaa cagcactttt
     4981 aaaattgatg tgaagcgtgc cgataaaaat ttcccaatgg atacgtatga attacaacgt
     5041 gaattgggtg gtaccgtatt gaaacaaatt gaaaatgttt ctgttaatgt caaacgtcca
     5101 gatcatgaaa ttcgagtgga agttagatta gatgcaattt atatgtatga agaagttgtt
     5161 ccgggttcag gtggattacc agttggtact ggtggtaaga cgttactaat gctttcaggc
     5221 ggtatagact caccagttgc tgggatggaa gtgatgagac gtggcgtaac aattgaagcg
     5281 attcatttcc atagtccacc atttacaagt gatcaagcaa aagaaaaagt tattgaattg
     5341 acacgtattt tagctgaacg tgttggacca attaaattgc atattgtacc atttacagaa
     5401 ttgcaaaaac aggtaaataa agttgtacat ccaagatata caatgacttc aacgagacgt
     5461 atgatgatgc gtgttgctga taaattagta catcaaatag gcgcttatgc catcgttaat
     5521 ggtgaaaatt taggtcaagt tgccagtcaa acacttcata gtatgtatgc aattaataat
     5581 gtaacttcta cccctgtatt acgtccttta ttaacttacg ataaagaaga aattattatt
     5641 aaatcgaaag aaattggtac atttgaaaca tctattcaac cttttgaaga ttgttgcaca
     5701 attttcaccc ctaaaaatcc agtaaccgaa ccaaactttg ataaggtagt tcaatatgaa
     5761 agtgtctttg attttgaaga gatgattaat cgtgctgttg aaaatattga aacacttgaa
     5821 ataactagtg attataaaac tattaaagaa cagcaaacaa accaattaat aaacgacttt
     5881 ttataaataa aatcttagag tgaatttaaa cataagggga tgttaaacta tggatttgaa
     5941 tttaacgatg attataatca taattttatt tggttttatc gcggcgttta tagattcggt
     6001 tgtagggggt ggtggtttaa tttctacgcc agcattatta gcaatcggtc taccaccatc
     6061 tgtggcttta ggtacaaata aattggcaag ttcatttggt tctttaacta gtgcgataaa
     6121 gtttataagg tccggtaaag tggacttata tgttgttgcc aaattatttg gttttgtatt
     6181 tttggcatct gcatgtggcg catatattgc aaccatggtt ccgtcacaaa tattgaaacc
     6241 tttaatcatt attgcacttt cgtcggtgtt tatattcaca ttacttaaaa aagattgggg
     6301 caatacacgt acgtttactc aatttacatt taagaaagcc atactatttg cagcactttt
     6361 tattttaatc ggcttttatg atggatttgt aggtggagga acaggttcat ttatgctttt
     6421 tgtattgtta atctttggtt ttgatttttt aagcgctgca ggaaatgcta aagttttgaa
     6481 ttttgcttct aatataggtg cgcttgtatt atttatggta ttaggacaag tagattatgt
     6541 aataggttta gttatggctg cgagtatgat tgtagggtca tatgctgggg ctcattttgc
     6601 tatcaaacaa ggtgttggct atgtgaaagt attatttatt attattactg cgattttaat
     6661 tttaaaaaat gcttttgatt atattcaaca atttgtataa aattaaattc gcttttgatt
     6721 aaagcatgaa aattgtaatc aaaccataaa ttgtcgtatg atgtagttag aattttaaaa
     6781 tgcaggaggt caagtatatg actgaaataa cattcaaagg tggaccaatc cacttaaaag
     6841 gtcaacaaat taatgaaggt gattttgcac ctgattttac agtgttagat aatgacttaa
     6901 atcaagtaac attagcagat tatgctggta aaaagaaatt aattagtgtg gtaccatcaa
     6961 ttgatacagg tgtttgtgat cagcagactc gcaaattcaa ctctgaagct tctaaagaag
     7021 aggggattgt gcttacaatc tcagcagact taccattcgc acaaaaaaga tggtgcgctt
     7081 cagcaggttt agacaatgtc attacattaa gtgaccaccg tgacttatca tttggtgaaa
     7141 actatggcgt tgttatggaa gaacttcgct tattagctcg tgcagtattt gtattagatg
     7201 cagataataa agttgtttat aaagaaatcg ttagtgaagg tactgatttc ccagattttg
     7261 atgctgcttt agctgcatac aaaaatattt aatcattaaa gagataaatc ttaaaatgta
     7321 tacatcgtgt ccatcgttgt caacagcatt aaaatagaat tgttttctat gattgctaag
     7381 acctatgggc actttttatt ggagagggac gaatatggca gaacaacaaa caattatgga
     7441 acgcttgttt cattcattag atgaaaaagc taaaacatta aataatgaaa atggccaaag
     7501 ttttattgaa aatcttgggc tagcaatgga acaagtttat acaaatgaac gaggattgtt
     7561 agaacaatcg acgttacaag atcgacgtaa agcattccaa tttgcatatt taagtttaat
     7621 gcaggaagaa aagatacaag caaatcatca aattacacca gattcaattg gattgatatt
     7681 aggattttta gttgagcgat ttatgaacaa tcaagaagaa ttacatattg ttgatattgc
     7741 aagtggtacc ggtcatttaa gtgctactgt aaaagaagtg ttacctgaaa ttgcggttat
     7801 gcatcattta attgaagttg atccagtttt atcacgtgtt agtgtacatt tagcaaactt
     7861 cttagaaatt cctttcgatg tgtatcctca agatgccatt atgccactac cgttagaaga
     7921 agcagatatc gttattggtg attttccagt aggctattat ccaattgatg aaagaagcag
     7981 ggactttaag ctaggtttcg aagaaggaca tagttattca cattatttat taatagaaca
     8041 agcaataaat gcattaaaag atgctggata tgcctttcta gtggtaccaa gtaatatttt
     8101 tacaggtgaa catgtaaaac agcttgaaaa atatattgca acagagacag aaatgcaagc
     8161 atttttaaat ttaccaccaa ctttatttaa aaatgaaaaa gcgcgaaaat ctatattaat
     8221 tttacaaaag aaaaaatcgg gtgaaacaaa gtcagttgaa gtattattgg caaatattcc
     8281 tgatttcaaa aatccttcac aatttcaagg atttatgaca gagttaaatc agtggatgga
     8341 cacaaatcgt cctaaaaaat aatctgtaat agtatagtca taaaactgta tgaatgataa
     8401 aatgaaaatg aaatagtata aaaagcaaac gatggaggat cattacttat gtcaaaatta
     8461 atcttggcta tcaacgctgg tagttcatca ttaaagtttc aattaatcag aatgcctgaa
     8521 gaggaattag taacaaaagg attagtagaa agaatcggat taaaagattc tatttttaca
     8581 attgaagtag acggggaaaa agttaaaaca gtacaagata tcaaggatca tgttgaagca
     8641 gttgatatca tgttagacgc atttaaaaaa cataatatca ttaatgatat taacgatatc
     8701 gatggaactg gtcaccgtgt tgtacatggt ggtgaaaaat tccctgaatc agcagcaatt
     8761 actgatgaag ttgaaaaaga aattgaagaa ttaagcgaat tagcaccgct tcacaaccca
     8821 gctaacttga tgggtattcg tgctttccgt aaattattac cgaatatccc tcatgtggcg
     8881 atttttgata cagcattcca tcaaacaatg cctgaaaaag catatctata tagcttgcca
     8941 tatcattatt ataaagatta cggtattcgt aagtatggct tccatggtac aagtcataaa
     9001 tttgtgtcac aaagagcggc agaaatgtta gataaaccaa ttgaagattt acgcattatt
     9061 tcttgtcata ttggtaacgg tgcatcaatt gcagctatcg atggcggtaa atctattgat
     9121 acatcaatgg gcttcacacc acttgctggt gtaacaatgg gtactcgttc tggtaatatt
     9181 gaccctgctt taattccatt tattatggaa aaaactggca aaactgctga acaagtattg
     9241 gaaattttaa acaaagaatc aggtttatta ggattatcag gtacatctag tgatttacgt
     9301 gacttatcag aagaagctga atcaggtaaa gcacgttcac aaatggcttt agatgtattt
     9361 gcttctaaaa ttcacaaata tattggatca tatgctgcga gaatgcatgg tgttgatgta
     9421 atcgtattta ctgcaggtat tggtgaaaat tcagtggaaa ttcgtgcaaa agttcttgaa
     9481 ggtttagaat ttatgggcgt ttactgggat cctaagaaaa atgaaaactt acaacgtggt
     9541 aaagaaggat ttattaacta tccgcattca cctgttaaag ttgtcgttat accgacagac
     9601 gaagaaagta tgattgctcg tgatgttatg acatacggtg gtctaaaata attttttaat
     9661 aaatattaag cgtctatctc cgctacaatt ataagtagga ggtagacgct ttttgtattg
     9721 tttgaaaatt aactagtgta atgtcttttt tcacgaatat cttaaaaaaa ttaagtagtc
     9781 gaagaagatt atgacaacat tattaagtga gttttattta taaacaataa aaaaccaagt
     9841 gcagaaagtt gcacttggtt cataatgatc aagtagttat ctaatgatca tctatattta
     9901 attttgatat ttttcacgta attgagttgt tgcaacttgt ggttggaagt ctgctggtaa
     9961 ctcttcagta cgaacaacta acacgtcaca tggagcatga cgaacgatag attctgatac
    10021 agaaccaaca ataaatcttt ccacggcatt taagcctgat gtaccactca taatcaagtc
    10081 tgcattaatt tcatgtgcaa gctttttagg aatgatagat ttaggagagc caaactctag
    10141 acgcgtttct acatctttaa caccagcgtt agtagctact tctttataac catttaataa
    10201 ttcttctgca aaatgcttag atttttcagt aaattgagca tcataaactt cataagaaga
    10261 atacgttctt gaatcaatta cattcacaat tgttaacttc gcatcgttac gtttagcaac
    10321 accaactgct ctgttaaatg cccattccgc ttcatgtgaa ccgtcaactg cgattaaaat
    10381 atttttgtaa gtaatcatag taagtgcctc cttgttcttt cttaatttta ttttaccaca
    10441 attttcggaa aagtcagtgt aataaccttt acaattttgt ggatttttga ttgcgctttc
    10501 aaaatgttcg cgttaagatg attatggagg tggatggtat gaaaattggt ataccaaggg
    10561 agattaaaaa taatgaaaat cgtgttggtt tatcaccaag tggtgtgcac gctttagtag
    10621 aaagtggaca tactgtttta gtggaaacaa atgcgggttc aggatcgttc tttgaagatg
    10681 tagattacaa agaagcaggt gctgagattg ttgctgaaca agcaaaagtt tgggatgtgg
    10741 atatggttat taaagttaaa gaacctcttg aatcagaata tccatatttt aaagaaggac
    10801 tagtattatt tacttatctt catctagcga atgaagaaaa attaacacaa gctttgatag
    10861 atagaaaagt aattagtatt gcatatgaga ctgtgcagtt accagaccga tctttaccat
    10921 tattatcacc aatgagtgag gtagcaggaa gaatgtcagc tcaagttggc gcagagttcc
    10981 tacaaaaact taatggtggt atgggaattc tactaggtgg cgttccagga gtacctaaag
    11041 gtaaagtaac tattatcggt ggtggtcaag caggaacaaa tgcagctaaa attgcactag
    11101 gactaggtgc agatgttacg attttagatg ttaatccaaa gcgtttacaa caattagatg
    11161 atttattcgg tggacgtgta catacaatta tgtcaaatcc gttgaatatt gagttgtatg
    11221 ttaaacaaag tgatttagta ataggtgcag ttttaattcc aggtgctaaa gcgccaagac
    11281 ttgtaacaga agacatgatt aaacaaatga aaaatgggtc agttattatt gacattgcta
    11341 ttgatcaagg cggtattttt gaaacaactg ataaaattac gacacatgat gatcctacat
    11401 atatcaaaca tggtgtggtt cattatgcag ttg
//
LOCUS       DAMZPP010000096        11425 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMZPP010000096 DAMZPP010000000
VERSION     DAMZPP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.096"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>11425
                     /locus_tag="POH73_001936"
     CDS             <1..>11425
                     /locus_tag="POH73_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GA module-containing protein"
                     /protein_id="HDI6887871.1"
                     /translation="QTTQSLNTAMTGLKRGVANHNQVVQSDNYVNADTNKKNDYNNAY
                     NHANDIINGNAQHPVITPSDVNNALSNVTSKEQALNGEAKLNAAKQEANTALGQLNNL
                     NNAQRQNLQSQINGAHQIETVNTIKQNATNLNSAMGNLRQAVADKEQVKRTEDYADAD
                     TAKQNAYNSAVSSAETIINQTTNPTMSVNDVNSATSAVTTNKNALNGDEKLAQSKTDA
                     ASAIDALPHLNNAQKADVKSKINAASNIAGVNTVKQQGTDLNTAMGNLQGAINDEQTT
                     LNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQNKTKDQVTEAMNQVNSAKNNLDGT
                     RLLDQAKQTAKQQLNNMTHLTTAQKTHLTNQINSGTTVAGVHTAQSNANTLDQAMNTL
                     RQSIANKDATKASEDYVDANNDKQTAYNNAVAAAETIINANSNPEMNPSTITQKAEQV
                     NSSKTALNGDENLATAKLNAKTYLNTLTSITDAQKNNLISQISSATRVSSVDTVKQNA
                     QHLDQAMASLQNGINNESQVKSSEKYRDADTNKQQEYDNAITAAKAILNKQHGPNTAQ
                     NAVEAALQRVKTAKNALNGDAKLIAAQNAAKQHLGTLTHITTAQRNDLTNQISQATNL
                     AGVESVKQSANSLDGAMGNLQTAINDKSGTLASQNFLDADEQKRNAYNQAVSNAETIL
                     NKQTGPNTAKTAVEQALNNVNSAKHALNGTQNLNNAKQAAITAINGASDLNQHQKDAL
                     KAQANGAQRVSNAQDVQRNATELNTAMGQLQHAIADKTTTLASSKFVNADSTKQNVYT
                     TKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQELNGDERLRVAKQNANTAIDALT
                     QLNTPQKAKLKEQVGQANRLEDVQSVQTNGQSLNNAMKGLRDSIANETTVKASQNYTD
                     ASPNNQSTYNSAVSNAKGIINQTNNPTMDASAITQATTQVNNAKNGLNGAENLRNAQN
                     TAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTAAKNAATELNTQMGNLEQAIHDQN
                     TVKQGVNFTDADKAKRDAYTSAVSRAETILNKTQGANTPKQDVEAAIQSVTSAKNALN
                     GDQNVTNAKNAAKHALNNLTSINNAQKRDLTTKIDQATTVSGVEAVSNTGTQLNTAMA
                     NLQNGINDKTNTLASENYHDADSDKKTAYTQAVTNAENILNKNSGSNLDKAAVENALS
                     QVTNAKGALNGNHNLEQAKSNANTTINGLQHLTTAQKDKLKQQVQQAQNVAGVDTVKS
                     SANTLNGAMGTLRNSIQDNAATKNGQNYLDATESNKTNYNNAVDSANGVINATSNPNM
                     DANAINQIATQVTSTKNALDGTHNLTQAKQTATNAIDGATNLNKAQKDALKAQVTSAQ
                     RVANVTSIQQTANELNTAMGQLQHGIDDENATKQTQKYRDAEQSKKTAYDQAVAAAKA
                     ILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKLAEAKAAAKQNLGTLNHITNAQRT
                     DLEGQINQATTVDGVNTVKTNANTLDGAMNSLQGSINDKDATLRNQNYLDADESKRNA
                     YTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRAKAALNGADNLRNAKTSATNTING
                     LPHLTQLQKDNLKHQVEQAQNVAGVNGVKDKGNTLNTAMGALRTSIQNDNTTKTSQNY
                     LDASDINKNNYNTAVNNANGVINATNNPNMDANAINGMANQVNTTKAALNGVQNLAQA
                     KTNATNTINNAHDLNQKQKDALKTQVNNAQRVSDANNVQHTATELNGAMTVLKAAIAD
                     KERTKASGNYVNADQEKRQAYDSKVTNAENIINGTPNATLTVNDVNSATSQVNAAKTV
                     LNGDNNLRVAKEHANNTIDGLAQLNNAQKAKLKEQVQSATTLDGVQTVKNSSQTLNTA
                     MKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAVTAAKAIINQTSNPTMEPNTITQA
                     TSQVTTKEQALNGAQNLAQAKTTAKNNLNNLTSINNAQKDALTRSIDGATTVAGVNQE
                     TVKATELNNAMHSLQNGINDEAQTKQTQKYLDAEPSKKSAYDQAVNAAKAILTKASGQ
                     NVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAKQTLGTLTHINNAQRTALDNEITQ
                     ATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTLQSQNYQDADDAKRTAYSQAVNAA
                     ATILNKTSGGNTPKADVERAMQAVTQANTALNGIQNLERAKQAANTAITNASDLNTKQ
                     KEALKAQVTSAGRVSVANGVEHTATELNTAMTALKRAIADKADTKASGNYVNADANKR
                     QAYDEKVTAAENIVSGTPTPTLTPSDVTNAATQVTNAKTQLNGNHNLEVAKQNANTAI
                     DGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQTLNTAMKGLRDSIANEATIKAEQ
                     NYTDASPNNRSEYDSAVTAAKAIIGQTSSPTMNAQEINQAKDQVTAKQQALNGQENLT
                     NAQINAKQHLNGLSDLTNAQKDAAKRQIEGATHVSEVTQAQNNADALNTAMTNLKNGI
                     QDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILNKVQGPNTAKDNVESALQNVQRAK
                     NDLNGNQNVANAKTTAKNALNNLTSINNAQNEALKSQIDSATTVAGVNQVSATASELN
                     TAMSNLQNGINDEAATKAAQKYTDADSDKQTAYNDAVTAAKTLLDKTAGTNDNKAAVE
                     QALQRVNTAKTALNGDARLNQAKNTAKQQLATMSHLTDAQKANLTSQIERGTTVAGVQ
                     GIQANAGTLNEAMNQLRQSIASKDATKASEDYHDANTDLQNAYNDAVTNAEGIISATN
                     NPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQTAKSDIGRLTDLNNAQRTAANAEV
                     DQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDNTKRSVNYTDADQPKQQAYDTAVT
                     QAEAITNANGSNANETQVQAALNQLNQAKNDLNGDNKVAQAKETAKRALASYSNLNNA
                     QSTAATSQIDNATTVAGVTAAQNTANELNTAMGQLQNGINDQNTVKQQVNFTDADQGK
                     KDAYTNAVTNAQGILDKAHGQNMTKAQVEAALNQVTTAKNALNGDANVRQAKSDAKAN
                     LGTLTHLNNAQKQDLTSQIEGATTVNGVNGVKTKAQDLDGAMQRLQSAIANKDQTKAS
                     ENYIDADPTNKTAFDNAITQAESYLNKDHGANKDKQAVEQAIQSVTSTENALNGDANL
                     QRAKTEATQAIDNLTHLNTPQKTALKQQVNAAQRVSGVTDLKNSATSLNNAMDQLKQA
                     IADHDTIVAGGNYTNASPDKQGAYTDAYNAAKNIVNGSPNVITNAADVTAATQRVNNA
                     ETGLNGDTNLATAKQQAKDALRQMTHLSDAQKQSITVQIDNATQVTGVQSVKDNATNL
                     DNAMNQLRNSIANKDEVKASQPYVDADRDKQNAYNTAVTSAENIINATSQPTLDPSAV
                     TQAANQVNTNKTALNGAQNLANKKQETTANINQLSHLNNAQKQDLNTQVTNAPNISTV
                     SQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTDADPEKQTAYNNAVTAAENIINQA
                     NGTNANQSQVEAALSTVTTTKQALNGDRKVTDAKNNANQTLSTLDNLNNAQKGAVTGN
                     INQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKDTTLGSQNFADADPEKKNAYNEAV
                     RNAENILNKSTGTNVSKDQVEAAMNQVNTTKAALNGTQNLEKAKQHANTAIDGLSHLT
                     NAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMDKLRQSIADNATTKQNQN"
BASE COUNT         4745 a         2076 c         2107 g         2497 t
ORIGIN      
        1 caaacgactc aaagcttaaa tactgctatg acaggtttaa aacgtggcgt tgctaatcat
       61 aaccaagtcg tacaaagtga taattatgtc aacgcagata ctaataagaa aaatgattac
      121 aacaatgcat acaaccatgc gaatgacatt attaatggta atgcacaaca tccagttata
      181 acaccaagtg atgttaacaa tgctttatca aatgtcacaa gtaaagaaca ggcattgaat
      241 ggtgaagcta agttaaatgc tgcgaagcaa gaagcgaaca cagcattagg tcaattaaat
      301 aacttgaata atgcacaacg tcaaaactta caatcgcaaa ttaatggtgc gcatcaaatc
      361 gagacagtta atacgattaa gcaaaatgca actaacttaa atagtgcaat gggtaactta
      421 agacaagctg ttgcagataa agagcaagtg aaacgtacag aagattatgc ggatgcagat
      481 acagctaaac aaaatgcata taatagcgcc gtttcaagtg ctgaaacaat cattaatcaa
      541 acaacaaatc caacgatgtc tgttaatgat gttaacagtg caacttcagc tgttactact
      601 aataaaaatg cattaaatgg tgatgaaaaa ttagcacaat ctaaaacaga tgctgcaagt
      661 gcaattgatg cattaccaca tttaaataat gctcaaaaag cagatgttaa atctaaaatt
      721 aatgctgcat caaatattgc tggtgtaaat actgttaaac aacaaggtac agatttaaat
      781 acagcgatgg gtaacttgca aggtgcaatc aatgatgaac aaacgacatt aaatagtcaa
      841 aattatcaag atgcgacacc aagtaagaaa acagcataca caaatgcggt acaagcagcg
      901 aaagatattt taaacaaatc aaatggtcaa aataaaacga aggatcaagt tacagaagcg
      961 atgaatcaag tgaattcggc taaaaataac ttagatggta cgcgtttatt agatcaagca
     1021 aagcaaacag cgaaacagca gttaaataat atgacgcatt taacaactgc tcaaaaaacg
     1081 catttaacaa atcaaattaa tagtggtact actgtcgctg gtgttcatac ggctcaatca
     1141 aatgcgaata cattagatca agcgatgaat acgttaagac aaagtattgc caacaaagat
     1201 gcgactaaag caagtgaaga ttacgtagat gctaataatg ataagcaaac agcatataac
     1261 aacgcagtag ctgctgccga aacgattatt aatgcgaata gtaatccaga aatgaatcca
     1321 agtacgatta cacaaaaagc agagcaagtg aatagttcta aaacggcact taacggtgat
     1381 gaaaacttag ctacggcaaa actaaatgca aaaacatact taaacacatt gacaagtatt
     1441 actgatgctc aaaagaacaa tttgattagt caaattagta gtgcgacaag agtgagtagt
     1501 gttgatactg taaaacaaaa tgcgcaacat ctagaccaag ctatggctag cttgcaaaat
     1561 ggtattaaca acgaatctca agtgaaatca tctgagaaat atcgtgatgc tgacacaaat
     1621 aaacaacaag agtatgataa tgctattact gcagctaaag cgattttaaa taaacaacat
     1681 ggtccaaaca ctgcgcaaaa tgcagttgaa gcagcattgc aacgtgtgaa aactgcgaaa
     1741 aatgcattga atggtgatgc caaattaatt gcagctcaaa acgcagcgaa acaacatcta
     1801 ggtactttaa cgcatatcac tacagcacaa cgcaatgatt taacaaatca aatttcacaa
     1861 gctacaaact tagctggtgt tgaatctgtt aagcaaagtg cgaatagttt agatggtgct
     1921 atgggtaact tacaaacggc tatcaacgat aagtcaggaa cattagcgag ccaaaacttc
     1981 ctagatgctg atgagcaaaa acgtaatgct tacaatcaag ctgtatcaaa tgccgaaaca
     2041 attttaaata aacaaactgg accgaataca gcgaaaactg cagtcgaaca agcacttaat
     2101 aatgttaata gtgcgaaaca tgcattaaat ggtacgcaaa acttaaataa tgcgaaacaa
     2161 gcagcgatta cagcaatcaa tggcgcatct gatttaaatc aacatcaaaa agatgcatta
     2221 aaagcacaag ctaatggtgc tcaacgcgta tctaatgcac aagatgtaca acgtaatgcg
     2281 actgaactga acacggcaat gggtcaatta caacatgcca tcgcagataa gacgactacg
     2341 ttagcaagca gtaaatttgt taacgccgat agcactaaac aaaatgttta cacaactaaa
     2401 gttaccaatg ctgaacatat tattagcggt acgccaacgg ttgttacgac accttcagaa
     2461 gtaacagctg cagctaatca agtaaacagc gcaaaacaag aattaaatgg tgacgaaaga
     2521 ttacgtgttg caaaacaaaa cgccaatact gctattgatg cattaacgca attaaataca
     2581 cctcaaaaag ctaaattaaa agaacaagtg ggacaagcca atagattaga agacgtacaa
     2641 tctgttcaaa caaatggaca atcattgaac aatgcaatga aaggcttaag agatagtatt
     2701 gctaacgaaa caacagtcaa agcaagtcaa aactatacag acgcaagtcc gaataaccaa
     2761 tcaacataca atagcgctgt gtcaaatgcg aaaggtatca ttaatcaaac taacaatccg
     2821 actatggatg ctagtgcgat tacccaagct acaacacaag tgaataatgc taaaaatggt
     2881 ttaaacggtg ctgaaaactt aagaaatgca caaaacactg ctaagcaaaa cttaaatacg
     2941 ttatcacact taacaaataa ccaaaaatct gcaatctcat cacaaattga tcgtgcaggt
     3001 catgtgagtg aggtaacagc tgctaaaaat gcagcaactg agttaaacac gcaaatgggc
     3061 aacttggaac aagctatcca tgatcaaaac acagttaaac aaggtgttaa cttcactgat
     3121 gcagataaag ccaaacgtga tgcgtataca agtgcggtaa gcagagcaga aacaattctg
     3181 aacaaaacgc aaggtgcaaa tacgcctaaa caagatgtag aagcggctat tcaaagtgtt
     3241 actagcgcta aaaatgcatt gaatggtgat caaaatgtca caaatgcgaa gaatgcagct
     3301 aaacatgcat taaataattt aacttcaatt aataatgcac aaaaacgtga cttaacaact
     3361 aaaattgatc aagcaacaac agtatctggt gttgaagcgg tatctaatac aggtacgcaa
     3421 ttgaatacag cgatggctaa cttgcaaaat ggtattaatg ataaaacgaa tactttagcg
     3481 agcgaaaact atcatgatgc tgattcagat aagaaaactg cttatactca agccgttacg
     3541 aacgcagaaa atattttaaa taaaaatagt ggatcaaatt tagacaaagc tgccgttgaa
     3601 aacgcgttgt cacaagtgac aaatgcgaaa ggtgccctaa atggtaacca taatttagag
     3661 caagctaaat caaatgcaaa cactactata aacggacttc aacatttaac aactgctcaa
     3721 aaagataaat tgaaacaaca agtgcaacaa gcacaaaatg ttgcaggtgt agatactgtt
     3781 aaatcaagtg ccaacacatt aaatggtgct atgggtacgt taagaaatag cattcaagat
     3841 aacgcagcta cgaaaaatgg ccaaaactat cttgatgcta cagaaagtaa caaaacaaac
     3901 tataacaatg ctgttgatag tgctaatggt gtcattaatg caacaagcaa tccaaatatg
     3961 gatgctaatg caattaacca aatcgctaca caagtgacat caacgaaaaa tgcattagat
     4021 ggtacacata atttaacgca agcgaaacaa acagcaacaa atgcgattga tggtgctact
     4081 aacttaaata aagcgcaaaa agatgcgtta aaagcacaag ttacaagtgc gcaacgtgtt
     4141 gcaaatgtaa caagtatcca acaaactgca aatgaactta atacagctat gggtcaatta
     4201 caacatggta ttgatgatga aaatgcaaca aaacaaactc aaaaatatcg tgatgcagag
     4261 caaagtaaga aaactgctta tgatcaagct gtagctgctg cgaaagcaat tttaaataaa
     4321 caaactggat caaattcaga taaagcagca gttgaccgtg cattacaaca agtaacaagt
     4381 acgaaagatg cattgaatgg ggatgctaaa ctggcagaag cgaaagcggc agctaaacaa
     4441 aacttaggca ctttaaacca tattacgaat gcacaacgta ctgacttaga aggccaaatc
     4501 aatcaagcga cgactgttga tggcgttaat actgtgaaaa caaatgccaa tacattagac
     4561 ggcgcaatga atagcttgca aggttcaatc aatgataaag atgcgacatt aagaaatcaa
     4621 aattatcttg atgcggatga atcaaaacga aatgcatata cgcaagctgt cacagcggct
     4681 gaaggcattt taaataaaca aactggtggt aacacatcta aagcagacgt tgataatgca
     4741 ttaaatgcag ttacaagagc gaaagcggct ttaaatggtg ctgacaactt aagaaatgcg
     4801 aaaacttcag caactaatac gattaatggt ttaccacact taacacaatt acaaaaagac
     4861 aacttgaagc atcaagttga acaagcgcaa aatgtagctg gtgtaaatgg tgttaaagat
     4921 aaaggtaata cattaaatac tgccatgggt gcattacgta caagtatcca aaatgataat
     4981 acgacgaaaa caagtcaaaa ttatcttgat gcatctgata tcaacaaaaa taattacaat
     5041 actgctgtaa ataatgcaaa tggtgttatt aatgcaacga acaatccaaa tatggatgct
     5101 aatgcgatta atggcatggc aaatcaagtc aatacaacaa aagcagcgtt aaatggtgta
     5161 caaaacttag ctcaagctaa aacaaatgcg acgaacacaa ttaataacgc acatgactta
     5221 aatcaaaaac aaaaagatgc attaaaaaca caagttaaca atgcacaacg tgtatctgat
     5281 gcaaataacg ttcaacatac agctactgaa ttgaacggcg cgatgacagt acttaaagca
     5341 gctattgctg ataaagaaag aacaaaagca agcggaaatt atgtcaatgc tgatcaagaa
     5401 aaacgtcaag cgtatgattc aaaagtgact aacgccgaaa atatcattaa tggtacaccg
     5461 aatgcgacat taacagtcaa tgacgtaaat agtgcaactt cacaagtgaa tgcagctaaa
     5521 acagtattaa atggtgataa caacttacgt gttgcgaaag agcatgccaa caatacaatt
     5581 gatggcttag cgcaattgaa taatgcacaa aaagcaaaat taaaagaaca agttcaaagt
     5641 gcaactacat tagatggcgt tcaaactgtt aaaaatagtt ctcaaacgtt gaatacagcg
     5701 atgaaaggct taagagatag tattgcgaat gaagcaacaa ttaaagcagg tcaaaactac
     5761 actgacgcaa gtccaaataa tcgtaacgag tacgacagcg cagttactgc agcaaaagca
     5821 atcattaatc aaacatcgaa cccaacgatg gaaccaaata ctattacgca agcaacatcg
     5881 caagtgacaa ctaaagaaca agcattaaat ggtgcgcaaa acttagctca agctaagaca
     5941 actgcgaaaa acaacttgaa taacttaaca tcaattaaca atgcacaaaa agatgcgtta
     6001 acgcgtagca ttgatggtgc aacaacagta gctggtgtaa atcaagaaac tgtcaaagca
     6061 actgaattaa ataacgcaat gcacagttta caaaatggta tcaatgatga ggcacaaaca
     6121 aaacaaactc agaaatacct agatgcagag ccaagtaaga aatcagctta tgatcaagca
     6181 gtaaatgcag cgaaagcaat tttaacaaaa gctagtggtc aaaatgtaga caaagcagca
     6241 gttgaacaag cattacaaaa tgtgaatagt actaagacag cgttgaacgg tgatgctaaa
     6301 ttaaatgaag ctaaagccgc tgcgaaacaa acgttaggta cattaacaca cattaataat
     6361 gcacaacgta cagcgttaga taatgaaatt acacaagcaa caaatgttga aggtgttaat
     6421 acagttaaag ccaaagcgca acaattagat ggtgctatgg gtcaattaga aacatcaatt
     6481 cgtgataaag acacgacgtt acaaagtcaa aattatcaag atgctgatga tgcgaaacga
     6541 actgcttatt ctcaagcagt aaatgcagca gctacaattt taaataaaac atctggcggt
     6601 aatacaccta aagcagatgt cgaaagagca atgcaagctg ttacacaagc caatactgca
     6661 ttaaacggta ttcaaaactt agaacgtgcg aaacaggctg ctaacacagc gattacaaat
     6721 gcttcggact taaatacaaa acaaaaagaa gcattgaaag cacaagtaac aagtgcagga
     6781 cgcgtatctg tagcaaatgg tgttgaacat actgctactg aattaaatac tgcgatgaca
     6841 gctttaaaac gtgccattgc tgataaagct gacacaaaag ctagtggtaa ctatgtcaat
     6901 gctgatgcga ataaacgcca agcatatgat gaaaaagtga cagctgcaga aaatatcgtt
     6961 agtggtacac caacaccaac gttaacacca tcagatgtta caaacgcagc aacgcaagta
     7021 acgaatgcga agacgcagtt aaacggtaat cataatttag aagtagcgaa acaaaatgcc
     7081 aacacagcaa ttgatggttt aacttcttta aatggtccgc aaaaagcaaa acttaaagaa
     7141 caagtgggtc aagcgacaac gttgccaaat gttcaaactg ttcgtgataa tgcacaaaca
     7201 ttaaacactg caatgaaagg tctacgagat agcattgcga atgaggcgac gattaaagca
     7261 gaacaaaact acacggatgc aagtcctaat aatcgtagtg aatacgatag tgcagtcact
     7321 gcagcaaaag caatcattgg tcaaacatca agtccaacaa tgaatgctca agaaatcaat
     7381 caagcgaaag accaagtgac agctaaacaa caagcgttaa acggtcaaga aaacttaaca
     7441 aacgcacaaa taaatgcgaa acaacatttg aatggcttaa gtgacttaac taatgcacaa
     7501 aaagatgcag caaaacgcca aatcgaaggt gcaacgcatg ttagtgaagt aacacaagcg
     7561 caaaataatg cggacgcatt aaatacagct atgacgaact tgaaaaatgg tattcaagat
     7621 caaaatacga ttaagcaagg tgttaacttc actgatgcag atgaagcgaa acgtaatgca
     7681 tatacaaatg cagtgacgca agctgaacaa attttaaata aagtacaagg tccaaatact
     7741 gcaaaagaca atgttgaatc tgcgttacaa aatgtacaac gtgctaaaaa cgatttgaac
     7801 ggtaatcaaa atgttgcaaa tgctaagaca actgcgaaaa atgcattaaa taatttaaca
     7861 tcaattaata atgcacaaaa cgaagcgttg aaatcacaaa ttgatagtgc gacaacagtt
     7921 gcaggtgtaa atcaagtgtc tgcaacggca tctgaattaa atacagcaat gagcaactta
     7981 caaaatggta ttaatgatga agcagctaca aaagcagctc agaaatatac tgatgcagat
     8041 agtgataaac aaactgcata caatgatgct gtaacagcag ctaaaacgtt attagataaa
     8101 acagctggca caaatgacaa taaagcagcc gttgaacaag cattacaacg tgtgaatact
     8161 gctaaaactg cattaaatgg tgacgcgcga ttaaatcaag cgaagaacac agctaaacaa
     8221 caattagcga caatgtcaca tttaactgat gctcaaaaag caaacttaac atcgcaaatc
     8281 gaacgcggta cgactgttgc aggtgttcaa ggtattcaag ctaatgccgg tactttaaat
     8341 gaagcaatga atcaattgag acaaagcatt gcttctaaag atgcgactaa agcgagcgaa
     8401 gattatcatg atgcgaatac agatttacaa aatgcataca atgatgcggt aactaatgct
     8461 gaaggtatta ttagtgcaac gaataaccct gaaatgaatc cagatacgat taatcaaaaa
     8521 gcgagccaag tgaacagtgc gaagtctgca ttgaacggtg atgaaaaatt agcagcagca
     8581 aaacaaactg cgaaatcaga catcggtcgt ttaacagact tgaacaatgc acaacgaact
     8641 gcggcaaatg ctgaagtgga tcaagcacca aatcttgcag ctgtcacagc ggctaaaaat
     8701 aaagcaacat cgttaaatac agcgatgggt aatttgaaac atgcacttgc tgaaaaggat
     8761 aatacgaaac gtagtgtcaa ttacacagat gcggatcaac ctaaacaaca agcgtatgat
     8821 actgcggtta cacaagcaga agcaattact aatgctaatg gcagcaacgc gaatgaaaca
     8881 caagttcaag cagcgcttaa ccaattgaat caagctaaaa atgacttgaa tggtgataat
     8941 aaagttgctc aagctaaaga aactgcgaaa cgtgcattag cttcatatag taacttgaat
     9001 aatgcgcaat caactgcagc aactagtcaa attgacaatg caacgacagt agcaggcgta
     9061 actgctgcac aaaatactgc taatgagtta aatacagcaa tgggtcaact tcaaaatggt
     9121 attaatgacc aaaacactgt taaacaacaa gtgaacttta cagatgctga ccaaggtaag
     9181 aaagatgctt acacaaatgc tgttacgaat gctcaaggta ttttagataa agcacacggt
     9241 caaaatatga cgaaagcaca agttgaagct gcattaaatc aagtaacgac tgctaagaat
     9301 gctttaaacg gtgacgcaaa tgtaagacaa gcaaaatcag atgcgaaagc aaacttaggc
     9361 acattaacac acttaaataa tgcacaaaaa caagatttaa catcacaaat cgaaggcgca
     9421 acaacagtca acggtgtaaa tggtgttaaa acgaaagcac aagacttaga tggtgcaatg
     9481 caacgattac aatcagcaat cgcaaataaa gatcaaacta aagcgagcga aaattacatc
     9541 gacgcagatc caactaacaa aacagcattt gataatgcca tcacacaagc agaatcatac
     9601 ttaaataaag atcacggtgc gaataaagat aagcaagctg ttgaacaagc aattcaaagt
     9661 gtaacgtcta ctgaaaatgc tttgaacggt gacgcaaact tacaacgtgc taaaactgaa
     9721 gctacgcaag ctatcgataa cttgacacat ttgaatacac cacaaaaaac agcattaaaa
     9781 caacaagtga atgctgcgca acgtgtatca ggtgtaactg atctgaaaaa tagtgctaca
     9841 tcacttaata atgcgatgga tcaattaaaa caagcaattg ctgatcatga cacaattgta
     9901 gctggtggta attacactaa cgcgagtcct gataagcaag gtgcttatac tgatgcatat
     9961 aatgctgcga aaaacattgt aaatggttca cctaatgtga ttacaaatgc agcagatgtt
    10021 acagcagcaa cacaacgtgt caataatgct gaaacaggtt taaacggtga tacaaactta
    10081 gcaactgcga agcaacaggc taaagatgca ttacgtcaaa tgacacattt atctgatgca
    10141 caaaaacaaa gtattactgt tcaaattgat aatgcgacac aagtaactgg cgttcaaagt
    10201 gtgaaagaca acgcgacaaa tcttgataat gcaatgaatc aacttcgaaa tagtattgcg
    10261 aataaagatg aagtaaaagc gagtcaacca tatgttgatg cagatagaga taaacaaaat
    10321 gcatacaaca cagctgttac aagtgctgaa aatatcatta atgcaacgag tcagccgaca
    10381 cttgatccat ctgcagtaac acaagcagct aatcaagtga acactaacaa aactgcgctt
    10441 aatggtgcac aaaacttagc aaataaaaag caagaaacaa ctgccaacat caaccaatta
    10501 agtcatttaa ataatgctca aaagcaagat ttaaatacgc aagtgacaaa tgcaccaaat
    10561 attagcacag taagtcaagt gaaaactaaa gctgaacaat tagatcaagc aatggaacgt
    10621 ttaatcaacg gaatccaaga caaagatcaa gtgaaacaaa gtgttaactt tacagatgca
    10681 gatccagaaa aacaaacagc atacaacaat gcggtaactg ctgctgaaaa tattattaat
    10741 caagcaaatg gtacaaatgc gaaccaatca caggttgaag cagcactttc aactgtaaca
    10801 actactaaac aagcattgaa tggtgataga aaagtaacag atgctaaaaa caatgcaaac
    10861 caaacattat ctacgttaga taacttaaac aatgcacaaa aaggtgctgt tactggaaac
    10921 atcaatcaag cgcacactgt tgctgaagta acgcaagcca ttcaaacagc tcaggaacta
    10981 aatacagcga tgggcaactt gaaaaatagc ttgaatgata aagacactac acttggcagt
    11041 caaaactttg cagatgcaga tccagagaag aaaaatgcat acaatgaagc ggttcgtaat
    11101 gctgaaaata ttttaaataa atctacaggt acaaacgtgt ctaaagatca agttgaagca
    11161 gctatgaatc aagtgaatac tacaaaagca gcgcttaatg gtactcaaaa ccttgaaaaa
    11221 gctaaacaac acgcaaatac agcgattgac ggtttaagcc atttaacaaa tgcacaaaaa
    11281 gaggcattaa aacaattggt acaacaatcg actactgttg cagaagcaca aggtaatgaa
    11341 caaaaagcaa acaatgttga tgcagcaatg gacaaattac gtcaaagtat tgcagataat
    11401 gcgacaacaa aacaaaacca aaatt
//
LOCUS       DAMZPP010000097        11344 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMZPP010000097 DAMZPP010000000
VERSION     DAMZPP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11344
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.097"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..513
                     /locus_tag="POH73_001937"
     CDS             <1..513
                     /locus_tag="POH73_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDI6887872.1"
                     /translation="KDRIIMLGSQIDDNVANSIVSQLLFLQAQDSEKDIYLYINSPGG
                     SVTAGFAIYDTIQHIKPDVQTICIGMAASMGSFLLAAGAKGKRFALPNAEVMIHQPLG
                     GAQGQATEIEIAANHILKTREKLNRILSERTGQSIEKIQKDTDRDNFLTAEEAKEYGL
                     IDEVMVPETK"
     gene            695..2329
                     /locus_tag="POH73_001938"
     CDS             695..2329
                     /locus_tag="POH73_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDI6887873.1"
                     /translation="MKGIELLNNPFLNKGTAFTNEERKQLGLEGLLPANVRTLEQQAE
                     QCYEQFKAKQTDFEKRLFLMAIFNRNRTLFYKLTSEHLVEFMPIIYDPVIAQSIEQYN
                     ENFSRPQDAVFLSVDRQDQIEQELRNVANGRDIRLIVVTDAEGILGIGDWGVDGVDIA
                     IGKLIVYTAAAGINPAQVLPVSLDVGTNNEQLLNDELYLGNRHKRVDDETYYTFVDKF
                     VSAVRKEYPNALLHWEDFGRGHAKNILDKYEDTLPTFNDDIQGTGIVTLAGVLGALNI
                     SKVDYTNQTFLVYGGGTAGMGITNILKDELIKQDVSEDKANQHFYIMDKQGLLFDDMD
                     DLTEAQQVFAKNRNEFSNTVNWKNLKEVIENIKPTVLIGTSTQTGAFTEDAVKEMLKH
                     TERPMIFPLSNPTKLAEATAQDLLKWTNGKALIGTGIPYNDIEYNGVNYSIGQANNAL
                     MYPGLGLGLIASKAKKVNQQILSAASHALGGMVNPDEPGAAVLPPVEKIHQCSRAIAK
                     AVAQSVIDQGLNAETIDDIDEAIEAEIWYPEYKSYI"
     gene            complement(2536..3438)
                     /locus_tag="POH73_001939"
     CDS             complement(2536..3438)
                     /locus_tag="POH73_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDI6887874.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNNKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKVLFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWVPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLISK"
     gene            4141..4770
                     /locus_tag="POH73_001940"
     CDS             4141..4770
                     /locus_tag="POH73_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HDI6887875.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            5264..6277
                     /locus_tag="POH73_001941"
     CDS             5264..6277
                     /locus_tag="POH73_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDI6887876.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKDYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            6330..7340
                     /gene="gap"
                     /locus_tag="POH73_001942"
     CDS             6330..7340
                     /gene="gap"
                     /locus_tag="POH73_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDI6887877.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            7479..8669
                     /locus_tag="POH73_001943"
     CDS             7479..8669
                     /locus_tag="POH73_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDI6887878.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            8791..9552
                     /locus_tag="POH73_001944"
     CDS             8791..9552
                     /locus_tag="POH73_001944"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDI6887879.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            9555..11072
                     /locus_tag="POH73_001945"
     CDS             9555..11072
                     /locus_tag="POH73_001945"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDI6887880.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVEDQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            11202..>11344
                     /gene="eno"
                     /locus_tag="POH73_001946"
     CDS             11202..>11344
                     /gene="eno"
                     /locus_tag="POH73_001946"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDI6887881.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEA"
BASE COUNT         4106 a         1716 c         2152 g         3370 t
ORIGIN      
        1 aaagaccgta ttattatgtt aggttcacaa attgatgaca acgtagcaaa ttcaatcgta
       61 tcacagttat tattcttaca agcacaagac tcagagaaag atatttattt atacattaat
      121 tcacccggtg gaagtgtaac agctggtttt gcgatttatg atacaattca acacattaaa
      181 cctgatgttc aaacaatttg tatcggtatg gctgcatcaa tgggatcatt cttattagca
      241 gctggtgcta aaggtaaacg ttttgcgtta ccaaatgcag aagtaatgat tcaccaacca
      301 ttaggtggtg ctcaaggaca agcaactgaa attgaaattg ctgcaaatca cattttaaaa
      361 acacgtgaaa aattaaatcg cattttatca gagcgtactg gtcaaagtat tgaaaaaata
      421 caaaaagaca cagatcgtga taacttctta actgcagaag aagctaaaga atatggctta
      481 attgatgaag tgatggtacc tgaaacaaaa taattcaaag taaagagtag actaagcagt
      541 ctgctctttt tgtatgatta aaccaattta taaataaata gttcaaaata ttttgagtaa
      601 aagcaaaaaa taacgggtaa aaatatttat aagaatttaa ttagacacgt tacgtctgcg
      661 gttctacaat ctaaaaaata taaaggatga ttttatgaaa ggcattgaat tacttaacaa
      721 tccattttta aacaaaggta cagcttttac taacgaagaa agaaaacagt tagggcttga
      781 aggattatta cctgcgaatg ttcgaacttt agaacaacaa gcagaacaat gttatgaaca
      841 attcaaggcg aaacaaaccg attttgagaa acgtttattt ttaatggcaa tttttaatcg
      901 taatcgtaca ttgttttaca aattgacttc tgagcattta gttgaattta tgccaattat
      961 ttatgaccct gttattgcac aatcgattga acaatataat gagaatttca gtagacccca
     1021 agatgcagta tttttatctg ttgatagaca agaccaaatt gaacaagaat tgcgcaacgt
     1081 tgcaaatggc agagatattc gattaatcgt agtaacagat gcggaaggta tattaggtat
     1141 cggcgattgg ggcgttgatg gtgtagatat tgcgatagga aaattaatag tatatacagc
     1201 ggcagctgga ataaatcccg cacaagtatt acctgtatct ttagatgtag gtactaataa
     1261 tgaacaatta ctgaatgatg aactttattt aggaaatcgt cataaacgtg tcgatgatga
     1321 gacatattac acatttgtag ataaatttgt gagtgcagtg cgtaaagaat atcctaatgc
     1381 actattacat tgggaagatt ttggtagagg gcatgctaaa aatattttag ataaatatga
     1441 agatacttta ccaacattta atgatgatat tcaaggtaca ggtatagtta cattagccgg
     1501 cgtactaggt gcattaaata tttctaaagt agactatacg aatcagacgt tcttagttta
     1561 tggtggtggt acagctggta tgggtattac caatatttta aaagatgaac taattaaaca
     1621 ggatgtttca gaagataaag caaatcagca tttctatatt atggataaac aaggattgtt
     1681 gtttgatgat atggatgact taactgaagc tcaacaagta tttgctaaaa ataggaatga
     1741 attttcaaat acagtaaact ggaaaaattt aaaagaagta attgaaaata ttaagcctac
     1801 tgttttaatc ggtacatcaa ctcaaacagg tgcatttaca gaagatgcag taaaagagat
     1861 gttaaaacat acagaacgtc caatgatttt cccattgtct aatcctacaa aattagcaga
     1921 agcaactgca caagatttat taaaatggac taatggaaaa gcattaattg gaactggtat
     1981 cccatataat gacattgaat acaatggtgt gaattacagt attggtcaag caaataatgc
     2041 attgatgtat cctggtttag gtttgggtct tattgcgtca aaagcgaaga aagtaaatca
     2101 acagattcta tcagctgcga gtcatgcgct tggtggcatg gtcaatccag atgaaccagg
     2161 agctgcagtc ttaccacctg ttgagaaaat acatcaatgt tctcgtgcca tagccaaagc
     2221 ggttgcacaa agtgtcattg atcaaggatt aaacgctgaa actattgatg atattgatga
     2281 agcaattgaa gctgaaattt ggtatcctga atacaaatca tacatttaat tttataaagt
     2341 agtaaaaggt catttgaaca ttttgtttca agtggccttt tcgtatttac tttaaaatta
     2401 atcaaccaat attcataagt gactttaaag ttatatctat atgtaatatc agcaaataga
     2461 aaagtctaga gagatttaac gaattatctc ctagactttt tgcttgaagt ggagtaggag
     2521 agatcaatgt cccactcact tactaattaa atcttcaagt gccattttta aattactata
     2581 tttaaattgg aatcccaatg cttgaatttt attaggtaat actttttgag tatccaatac
     2641 tactgttgac atttgaccaa gtatgagacg cattgcaaga cttggtaccc aagtttcgtg
     2701 aggcttatgc atagctcttg ctaaagtgta gccaaataaa ttttgacgct caggtatagg
     2761 tgcagttaaa ttaaacggac cactagctga ctcgttattt attaaaaata aaatagcttg
     2821 aattaaatca ttgatatgaa tccatgaata ccattgttga ccagaaccta atttaccacc
     2881 aatgtaatat tcgtatggta gtttcattgt ttgtaacgca ccgccttcat tcgataaaat
     2941 tataccgaaa cgaccgatga caactcgtgt acctaattgt tcaaattgtt gtgcgaaacg
     3001 ttcccattga tacacaatat ctgataagaa atcaaatggt aaagttttat aaacttctgt
     3061 gtaactcata aataaatcag gaggatagta accagtggca ctagcattaa ataaaacttt
     3121 aggtgcttta ttacgtgatt taaacaattc atataaagct tgcgtagatt gaattctact
     3181 tagcattagc gtttgtttat attccggtgt ccatcgttta ttcaatgtag cacctgctaa
     3241 gttgatgacc acatcgatat tttgaggaac tttgtgttcc cacccagatt tagcccagtt
     3301 gacatatgaa attttcttat tatttgaaat ttggtcgtgt cgcgttaata tcgtgatatg
     3361 tgaatctgat tttttgattt cattaactaa ttgagatcca accataccag tcccaccagt
     3421 aattaagtat tgtttcatta tcattcaccc catgtaattt tgtatttagt tactatttaa
     3481 taatcattat tgttgttcaa aggttataca ttatttagac aataatatgt caataacttt
     3541 ttttgaattg tattttaata aaaatgatat aagttataat aaaggtgtac cttcgataac
     3601 gaataaacat ctcttaaaag tatgtgtaaa acgctgcatg atacaaacga aggtaaaaat
     3661 ttgactccct ttagtagtgg acccgtacgt ttatcgtgcg ggtcgttttt atttatatct
     3721 tattcccgtt atacatcaat tcaaagcatt aattttttaa acaaatttaa gaatagatag
     3781 taatataaca atctaaacat aaaaactttt aacacaacac ttaaactaat gctttaattt
     3841 tcaatacgta gctataattt gttgtaaaat caaaaaggtt aatatgttaa ttttcaaaaa
     3901 aaggctcaaa atatgtttga tttagttatt aaatgttaag atatataaga ctactatttc
     3961 tttgtaaaaa tgaatccgat ttacgagtga gtaatagtga atatagtttt aagttgaaga
     4021 aggcaaaaag agtaaatgtt tattaatgtt tgtagaaaca aggtaagcaa attagttgtg
     4081 aaaatgttaa tagttacgtg ataatttcta tatttaaatt agtttgaagt gagggagagt
     4141 atgtcgaatc aaaattacga ctacaataaa aatgaagatg gaagtaagaa gaaaatgagt
     4201 acaacagcga aagtagttag cattgcgacg gtattgctat tactcggagg attagtattt
     4261 gcaatttttg catatgtaga tcattcgaat aaagctaaag aacgtatgtt gaacgaacaa
     4321 aagcaggaac aaaaagaaaa gcgtcaaaaa gaaaatgcag aaaaagagag aaagaaaaag
     4381 caacaagagg aaaaagagca gaatgagcta gattcacaag caaaccaata tcagcaattg
     4441 ccacagcaga atcaatatca atatgtgcca cctcagcaac aagcacctac aaagcaacgt
     4501 cctgctaaag aagagaatga tgataaagca tcaaaggatg agtcgaaaga taaggatgac
     4561 aaagcatctc aagataaatc agatgataat cagaagaaaa ctgatgataa taaacaacca
     4621 gctcagccta aaccacagcc gcaacaacca acaccaaagc caaataataa tcaacaaaac
     4681 aatcaatcaa atcaacaagc gaaaccacaa gcaccacaac aaaatagcca atcaacaaca
     4741 aataaacaaa ataatgctaa tgataagtag tatttagtca aacaaaaatg aaccagtatg
     4801 acagacaaca caattaatta ggttgtctcg aatattggtt cttattttta taattgttaa
     4861 ttaggggaga gatgatactt aaatagttag ttgtttattt tgcagatagt gaaatttatt
     4921 ttttcagtca actactggca atgtaacttt tcagattcta gagcattgat ttatgtccta
     4981 gtctcaaata ataagcaaat gattagagaa tcagtataag ggtatacatt tgactacagc
     5041 gaataaaata aaacgttttg ttgtataata aatcgaaaat atattgcaag cgctttatca
     5101 aattatttag aaaatttaag ttttatgctt gcaatttttg aaatagaata gtactattgc
     5161 aagtgtaaag aggttaattt ttgtcccacg tgggacttaa aaaggcaacc actggttgtg
     5221 acatatcctt atttacattt ataaatataa ggaggaggta gtagtgaaag acttattgca
     5281 agcacagcaa aagcttatac cggatctcat agataaaatg tataaacgtt tttctattct
     5341 tactactatc tcaaaaaatc agcctgtcgg acgtcgaagt ttaagcgaac atatggatat
     5401 gactgaacgt gtactgcgtt ctgaaacaga tatgcttaag aaacaagatt tgataaaagt
     5461 taagcctacc ggaatggaaa ttacagctga aggtgagcaa ctgatttcgc aattgaaaga
     5521 ttactttgat atctatgcag atgacaatcg tctgtcagaa ggtattaaga ataaatttca
     5581 aattaaggaa gttcatgttg ttcctggtga tgctgataat agccaatctg ttaaaacaga
     5641 attaggtagg caagcaggtc aattacttga aggcatatta caagaagatg cgatagttgc
     5701 tgtaactggc ggatccacga tggcatgtgt tagtgaagca attcatttat taccatataa
     5761 tgtattcttc gtaccagcca gaggtggact aggcgaaaat gttgtctttc aggcaaacac
     5821 aattgcagcc agtatggcac aacaagctgg cggttattat acgacgatgt atgtacctga
     5881 taatgtcagc gaaacaacat ataacacatt gttgttagag ccatcagtca taaacacttt
     5941 agacaaaatt aaacaagcaa acgttatatt acacggcatt ggtgatgcgc tgaagatggc
     6001 gcatcgacgt caatcacctg aaaaggtcat tgaacaactt caacatcatc aagctgtcgg
     6061 agaggcattt ggttattatt ttgatacaca aggtcaaatt gtccataagg ttaaaacaat
     6121 tggacttcaa ttagaagacc ttgaatcaaa agactttatt tttgcagttg caggaggcaa
     6181 atcgaaaggt gaagcaatta aagcatactt gacgattgca cccaagaata cagtgttaat
     6241 cactgatgaa gccgcagcaa agataatact tgaataagag ataaaaagtt taatactttt
     6301 taaatatcat tttaaaggag gccattataa tggcagtaaa agtagcaatt aatggttttg
     6361 gtagaattgg tcgtttagca ttcagaagaa ttcaagaagt agaaggtctt gaagttgtag
     6421 cagtaaacga cttaacagat gacgacatgt tagcgcattt attaaaatat gacactatgc
     6481 aaggtcgttt cacaggtgaa gtagaggtag ttgatggtgg tttccgcgta aatggtaaag
     6541 aagttaaatc attcagtgaa ccagatgcaa gcaaattacc ttggaaagac ttaaatatcg
     6601 atgtagtatt agaatgtact ggtttctaca ctgataaaga taaagcacaa gctcatattg
     6661 aagcaggcgc taaaaaagta ttaatctcag caccagctac tggtgactta aaaacaatcg
     6721 tattcaacac taaccaccaa gagttagatg gttctgaaac agttgtttca ggtgcttcat
     6781 gtactacaaa ctcattagca ccagttgcta aagttttaaa cgatgacttt ggtttagttg
     6841 aaggtttaat gactacaatt cacgcttaca caggtgatca aaatacacaa gacgcacctc
     6901 acagaaaagg tgacaaacgt cgtgctcgtg cagcagcaga aaacatcatc cctaactcaa
     6961 caggtgctgc taaagctatc ggtaaagtta ttcctgaaat cgatggtaaa ttagatggtg
     7021 gtgcacaacg tgttcctgta gctacaggtt cattaactga attaacagta gtattagaaa
     7081 aacaagacgt aacagttgaa caagttaacg aagctatgaa aaatgcttca aacgaatcat
     7141 tcggttacac tgaagacgaa atcgtttctt cagacgttgt aggtatgact tacggttcat
     7201 tattcgacgc tacacaaact cgtgtaatgt cagttggtga ccgtcaatta gttaaagttg
     7261 cagcttggta tgataacgaa atgtcatata ctgcacaatt agttcgtaca ttagcatact
     7321 tagctgaact ttctaaataa ttttagtata gtttttattc aaatacgcta gtgctcagaa
     7381 ctatttagca ttaattaaag cttatgagta agcggggagc acaaacgctt ctccgcttat
     7441 ttttatataa aatttcctaa ttacaaggag gaaacaccat ggctaaaaaa attgtttctg
     7501 atttagatct taaaggtaaa acagtcctag tacgtgctga ttttaacgta cctttaaaag
     7561 acggtgaaat tactaatgac aaccgtatcg ttcaagcttt acctacaatt caatacatca
     7621 tcgaacaagg tggtaaaatc gtactatttt cacatttagg taaagtaaaa gaagaaagtg
     7681 ataaagcaaa attaacttta cgtccagttg ctgaagactt atctaagaaa ttagataaag
     7741 aagttgtttt cgtaccagaa acacgcggcg aaaaacttga agcagctatt aaagacctta
     7801 aagaaggcga cgtattatta gttgaaaata cacgttatga agatttagac ggtaaaaaag
     7861 aatctaaaaa tgatccagaa ttaggtaaat actgggcatc tttaggtgat gtgtttgtaa
     7921 atgatgcttt tggtactgcg catcgtgagc atgcatctaa tgttggtatt tctacacact
     7981 tagaaactgc agctggattc ttaatggata aagaaattaa gtttatcggt ggcgtagtta
     8041 acgatccaca taaaccagtt gttgctattt taggtggagc aaaagtatct gacaaaatta
     8101 atgtcatcaa aaacttagtt aacatcgctg ataaaattat catcggcgga ggtatggctt
     8161 atactttctt aaaagcgcaa ggtaaagaaa ttggtatttc attattagaa gaagataaaa
     8221 tcgacttcgc aaaagattta ttagaaaaac atggtgataa aattgtatta ccagtagaca
     8281 ctaaagttgc taaagaattt tctaatgatg ccaaaattac tgtagtacca tctgattcaa
     8341 ttccagcaga ccaagaaggt atggatattg gaccaaacac tgtaaaatta tttgcagatg
     8401 aattagaagg tgcgcacact gttgtatgga atggacctat gggtgtattc gagttcagta
     8461 actttgcaca aggtacaatt ggtgtatgta aagcaattgc aaaccttaaa gatgcaatta
     8521 cgattatcgg tggcggtgat tcagctgcag cagcaatctc tttaggtttt gaaaatgact
     8581 tcactcatat ttcaactggt ggcggagcgt cattagagta cctagaaggt aaagaattgc
     8641 ctggtatcaa agcaatcaat aataaataat aaagtgatag tttaaagtga tgtggcatgt
     8701 ttgtttaaca ttgttacggg aaaacagtca taagatgaca tcgtgtttca tcacttttca
     8761 aaaatattta caaaacaagg agtgtcttta atgagaacac caattatagc tggtaactgg
     8821 aaaatgaaca aaacagtaca agaagcaaaa gacttcgtca atgcattgcc aacattacca
     8881 gattcaaaag aagtagaatc agtaatttgt gcaccagcaa ttcaattaga tgcattaact
     8941 actgcagtta aagaaggaaa agcacaaggt ttagaaatcg gtgctcaaaa tacgtatttc
     9001 gaagataacg gtgcgttcac aggtgaaacg tctccagttg cattagcaga tttaggcgtt
     9061 aaatacgttg ttatcggtca ttctgaacgt cgtgaattat tccacgaaac agatgaagaa
     9121 attaacaaaa aagcgcacgc tattttcaaa catggaatga ctccaattat ttgtgttggt
     9181 gaaacagacg aagagcgtga aagtggtaaa gctaacgatg ttgtaggtga gcaagttaag
     9241 aaagctgttg caggtttatc tgaagatcaa cttaaatcag ttgtaattgc ttatgaacca
     9301 atctgggcaa tcggaactgg taaatcatca acatctgaag atgcgaatga aatgtgtgca
     9361 tttgtacgtc aaactattgc tgacttatca agcaaagaag tatcagaagc aactcgtatt
     9421 caatatggtg gtagtgttaa acctaacaac attaaagaat acatggcaca aactgatatt
     9481 gatggggcat tagtaggtgg cgcatcactt aaagttgaag atttcgtaca attgttagaa
     9541 ggtgcaaaat aatcatggct aagaaaccaa ctgcgttaat tattttagat ggttttgcga
     9601 accgcgaaag cgaacatggt aatgcggtaa aattagcaaa caagcctaat tttgatcgtt
     9661 attacaacaa atatccaacg actcaaatcg aagcgagtgg cttagatgtt ggactacctg
     9721 aaggacaaat gggtaactca gaagttggtc atatgaatat cggtgcagga cgtatcgttt
     9781 atcaaagttt aactcgaatc aataaatcaa ttgaagacgg tgatttcttt gaaaatgatg
     9841 ttttaaataa tgcaattgca cacgtgaatt cacatgattc agcgttacac atctttggtt
     9901 tattgtctga cggtggtgta cacagtcatt acaaacattt atttgctttg ttagaacttg
     9961 ctaaaaaaca aggtgttgaa aaagtttacg tacacgcatt tttagatggt cgtgacgtag
    10021 atcaaaaatc cgctttgaaa tacatcgaag agactgaagc taaattcaat gaattaggta
    10081 ttggtcaatt tgcatctgtg tctggccgtt attatgcaat ggaccgtgac aaacgttggg
    10141 aacgtgaaga aaaagcttac aatgctattc gtaactttga tgccccaact tatgcaactg
    10201 ccaaagaagg tgtggaagca agttataatg aaggcttaac tgacgaattc gtagtaccat
    10261 tcatcgttga ggatcaaaat gacggtgtta atgatggaga tgcagtgatc ttctataatt
    10321 tccgacctga tagagcggca caattatcgg aaatttttgc gaacagagca ttcgaaggat
    10381 ttaaagttga acaagttaaa gacttattct atgcaacatt cactaagtat aatgacaata
    10441 tcgatgcggc tatcgtcttc gaaaaagtcg atttaaataa tacaatcggt gaaattgcac
    10501 aaaataacaa tttaactcaa ttacgtattg cagaaactga aaaatatcct cacgttactt
    10561 actttatgag tggtggacgt aatgaggaat ttaaaggtga acgtcgtcgt ttgattgatt
    10621 cacctaaagt tgcaacgtat gacttgaaac cagaaatgag tgcttatgaa gttaaagatg
    10681 cattattaga agagttaaat aaaggtgact tggacttaat tattttaaac tttgctaacc
    10741 ctgatatggt tggacatagt ggtatgcttg agccgacaat caaagcaatc gaagcggttg
    10801 atgaatgttt aggcgaagtg gttgataaga ttttagacat ggacggttat gcaattatta
    10861 ctgctgacca tggtaactct gatcaagtat tgacggatga tgatcaacca atgacgacgc
    10921 atacaacgaa cccagtacca gtgattgtaa caaaagaagg cgttacactt agagaaactg
    10981 gtcgtttagg tgacttagca cctacattat tagatttatt aaatgtagaa caacctgaag
    11041 atatgacagg tgaatcttta attaaacact aatattgtaa aagatgttaa gtaaacgctt
    11101 aatgacactt attttttgaa aataatagta atatcatttt gttaaatgaa agaataaagc
    11161 tataataatt atagaataac tatttaaagg agattataaa catgccaatt attacagatg
    11221 tttacgctcg cgaagtctta gactctcgtg gtaacccaac tgttgaagta gaagtattaa
    11281 ctgaaagtgg cgcatttggt cgtgcattag taccatcagg tgcttcaact ggtgaacacg
    11341 aagc
//
LOCUS       DAMZPP010000098        11312 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMZPP010000098 DAMZPP010000000
VERSION     DAMZPP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.098"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..513
                     /locus_tag="POH73_001947"
     CDS             <1..513
                     /locus_tag="POH73_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDI6887882.1"
                     /translation="HNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRMEQHTLEN
                     DITVINDAYNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEK
                     HIDVLYTFGNEAKYIYDSGQQHVEKAQHFNSKDDMIGVLTSDLKAHDRVLVKGSRGMK
                     LEEVVNALIS"
     gene            1030..2550
                     /locus_tag="POH73_001948"
     CDS             1030..2550
                     /locus_tag="POH73_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6887883.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELIEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(2906..3601)
                     /locus_tag="POH73_001949"
     CDS             complement(2906..3601)
                     /locus_tag="POH73_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6887884.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTAENATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(3601..6258)
                     /locus_tag="POH73_001950"
     CDS             complement(3601..6258)
                     /locus_tag="POH73_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDI6887885.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKLGYIEPHQRDETNALAEQLPKIATNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KSRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KRRDFFNKPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSYIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYAITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNKTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVDLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SDEQIEFAVIDEGPGISIEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIDTDFNEGDI"
     gene            6396..6506
                     /gene="kdpF"
                     /locus_tag="POH73_001951"
     CDS             6396..6506
                     /gene="kdpF"
                     /locus_tag="POH73_001951"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDI6887886.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            6530..8206
                     /gene="kdpA"
                     /locus_tag="POH73_001952"
     CDS             6530..8206
                     /gene="kdpA"
                     /locus_tag="POH73_001952"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HDI6887887.1"
                     /translation="MEIILFLTMMVMIAYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGVITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESGVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIHGANMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPLGGLGPMVLMMLNVVFGGEGVGLMNLLIYVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMISSSLVN
                     KKSYHQDKHTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            8225..10252
                     /gene="kdpB"
                     /locus_tag="POH73_001953"
     CDS             8225..10252
                     /gene="kdpB"
                     /locus_tag="POH73_001953"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDI6887888.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNPKQLIKNPIMFVVEVGMLL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMMEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESICMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFAELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            10272..10832
                     /locus_tag="POH73_001954"
     CDS             10272..10832
                     /locus_tag="POH73_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDI6887889.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RVKHHVKFDNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHISTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            11041..>11312
                     /locus_tag="POH73_001955"
     CDS             11041..>11312
                     /locus_tag="POH73_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887890.1"
                     /translation="MSDYTKLSKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPQYLIYILFVLCTLTVLKLIIKPLWQYHCRFYQVDQLSVQYRTSF"
BASE COUNT         3744 a         1625 c         2076 g         3867 t
ORIGIN      
        1 cataatatga aaaatgcgac gattgccatt gcggttggtc atgaattagg tttgacatat
       61 aacacaatct atcaaaattt aaaaaatgtc agcttaactg gtatgcgtat ggagcaacat
      121 acattagaaa atgatattac tgtgataaat gatgcctata atgcaagtcc tacaagtatg
      181 agagcagcta ttgatacact gagtaccttg acagggcgtc gcattctaat tttaggagat
      241 gttttagaat taggtgaaaa tagcaaagaa atgcatatcg gtgtaggtaa ttatttagaa
      301 gaaaagcata tagatgtgtt gtatacgttt ggtaatgaag cgaagtatat ttatgattcg
      361 ggccagcaac atgtcgaaaa agcacaacac ttcaattcta aagacgatat gataggagtt
      421 ttaacaagcg atttaaaagc gcatgaccgt gtattagtta aaggctcacg tggtatgaag
      481 ttagaagaag ttgtaaatgc tttaatttca tagagattag tcgagcgacc ttttacttat
      541 aaaaatgatt tgaattaata ctaaaagatt acaaagatga ggtggttttg tgtgtaaata
      601 caaaattgcc tttttctttt tatgttaaat ctataaattt gaaactaaat caaggttaat
      661 tctatgtaca cactatatat aggaagtagt ttgaatgttt atataatatt ttacaaaaag
      721 atgtagtatt ataatgtcta atttcacatg tgtttcagta aaatttgttg tggaatgtta
      781 acgatatacg tattttataa aaattttttt ataatgatta ttcgaatgat gcgtaacgct
      841 tacatcttat ctaatgctag ctttttgaca aaaatatgac aatcaattaa tgtgattcta
      901 ataaatattc gcaaattgct ttattgcgat taaatttttt tggtggtact atatagaagt
      961 tgatgaaata ttaatgaact tatatgcaaa agtatattga gaaataaaca ggtaaaaagg
     1021 agaattattt tgcaaaattt taaagaacta gggatttcgg ataatacggt tcagtcactt
     1081 gaatcaatgg gatttaaaga gccgacacct atccaaaaag acagtatccc ttatgcgtta
     1141 caaggaattg atatccttgg gcaagctcaa accggtacag gtaaaacagg agcattcggt
     1201 attcctttaa ttgagaaagt agtagggaaa caaggggttc aatcgttgat tttagcacct
     1261 acaagagaat tggcaatgca ggtagctgaa caattaagag aatttagccg tggacaaggt
     1321 gtccaagttg ttactgtatt cggtggtatg cctatcgaac gccaaattaa agccttgaaa
     1381 aaaggcccac aaatcgtagt cggaacacct gggcgtgtta tcgaccattt aaatcgtcgc
     1441 acattaaaaa cggacggaat tcatactttg attttagatg aagctgatga aatgatgaat
     1501 atgggattca tcgatgatat gagatttatt atggataaaa ttccagcagt acaacgtcaa
     1561 acaatgttgt tctcagctac aatgcctaaa gcaatccaag ctttagtaca acaatttatg
     1621 aaatcaccaa aaatcattaa gacaatgaat aatgaaatgt ctgatccaca aatcgaagaa
     1681 ttctatacaa ttgttaaaga attagagaaa tttgatacat ttacaaattt cctagatgtt
     1741 catcaacctg aattagcaat cgtattcgga cgtacaaaac gtcgtgttga tgaattaaca
     1801 agtgctttga tttctaaagg atataaagct gaaggcttac atggtgatat tacacaagcg
     1861 aaacgtttag aagtattaaa gaaatttaaa aatgaccaaa ttaatatttt agtcgctact
     1921 gatgtagcag caagaggact agatatttct ggtgtgagtc atgtttataa ctttgatata
     1981 cctcaagata ctgaaagcta tacacaccgt attggtcgta cgggtcgtgc tggtaaagaa
     2041 ggtatcgcgg taacatttgt aaatccaatt gaaatggatt acattagaca aattgaagat
     2101 gctaatggta gaaaaatgag cgcacttcgt ccaccacatc gtaaagaagt acttcaagca
     2161 cgtgaagatg acatcaaaga aaaagttgaa aactggatgt ctaaagagtc agaatcacgc
     2221 ttgaaacgca tttctacaga gttgttaaat gaatataacg atgttgattt agttgctgca
     2281 cttttacaag agttaataga agcgaacgat gaagttgaag ttcaattaac ttttgaaaaa
     2341 ccattatctc gcaaaggccg taacggtaaa ccaagtggtt ctcgtaacag aaatagtaag
     2401 cgtggtaatc ctaaatttga cagtaagagt aaacgttcaa aaggatactc aagtaagaag
     2461 aaaagtacaa aaaaattcga ccgtaaagag aagagtagcg gtggaagcag acctatgaaa
     2521 ggtcgcacat ttgctgacca tcaaaaataa tttatagatt aagagcttaa agatgtaatg
     2581 tcttgagctc ttttttgttt tcaataactg attctatgta gatatcaaaa tactaacatt
     2641 ttaaaggtta aatatttaat tggattgaga gctgtgtgcg gttatatcat tctgtttaaa
     2701 tataaatatg aatctagaat ataacaacca ctaaaactaa gaataattga ccaaaaataa
     2761 attttatgaa agtaatttcg gtatcatgtg cttgtaaaaa acaatagggc attttgctga
     2821 cactagtgcg caataaataa taagttgaat ataaaaaaga tcactgccaa tcattcgttt
     2881 aatggcagcg atctttttta tttaattatt tctctttcca ctgcaacatt tgataaccaa
     2941 tgcgtggatg tgttttaata atatcttttg cgtcctcgtg acattgtgaa agtttttgtc
     3001 ttaatgatgt catatgcact cttaaactag gcatttcagt cttattaaca tagccatata
     3061 ttcgttttaa tatcatctca taagtgagta cttttccttt atgatttgac aatagttcta
     3121 acaagctaaa ttcatttggt gtcaaatgta cctcctgatt attaataaca acagatttgg
     3181 agccaaagtc gatgcttaac aaaccgttag taaatacaat gttagtttct tgatgtgact
     3241 tagcgattct ttcgatgact cgaattcgtg cccgaagctc atcaacatta aaaggcttag
     3301 tcatatagtc attcgcaccg ttatctaaag cttgaataat tgtttgttct tcttgtcttg
     3361 cacttattac aatgatagga atgtcagtat gttgcctgat ttctgaaatc aaacataatc
     3421 catctttatc tggtaaacct aaatctaata aaatgacatc tggtttatca atttgaattt
     3481 taaagtgtgc ttgtgtggca ttttcggctg tagttacatt gtaataatct aaagttaatg
     3541 caacatcaag taaatgtgtg attgcgtgat catcttcaat tatcaatatt ttagattgca
     3601 ttatatgtct ccttcattaa agtctgtatc tatattgaaa tagaatatac tgccatgtgg
     3661 ttggttcggt ttatattgta ggtttgattg atgtttgtgt aggatagtct gtactaaata
     3721 taagcctagt cccatgcttt ctttttggtt atctttaaaa tatttatttg accctgtgta
     3781 aaaaggctcg aatatctttt gttgttcttc tatactaatt ccaggtcctt cgtctataac
     3841 agcaaattcg atttgttcat cgctagcata acgaatagat aaattgattt tggtgtcagt
     3901 agaagtgtgt ttaactgcat tttcaatcaa attgaataaa gcttgtaaaa tcaacttact
     3961 gtcaatgtgt ataaactgta aatctactga ggatgataca gttatacgct tttttaaatg
     4021 gcgacgttct aaaatcatat cgatttcttc tactaattca ctaacgagat aaggttgcaa
     4081 ttttatctga acatttgatg actgtaattt tgttaatgat aaaatatttg tcactaatag
     4141 atataaatac tgactttctt gaaaactatg cacaagtaat tgttcctttt ctatgataga
     4201 catatcttta ctatgtgaca ctaaaatatc taaatttccc ataattgttg ttaacggtgt
     4261 acgtatgtca tgcgaaattg atcttaaaaa atttgaatgt gtcagttgac gttcagcctg
     4321 taacatggat tctcttgttt gtttaagcaa cgtcacattt tcaacggcga gagaaagttc
     4381 atttaacatt gattctaata ttgatgcatc atatggatta atcacttgag aactttggta
     4441 atcaatggct agaatgcctt taatcggaga tgtgccaatt ggtatcaacc atttattaat
     4501 gcctggaaat gtatctgttg ttgcaccagc ttgtctttca tttttaatta cccagcttaa
     4561 tgcttgttca tgctgttgag tcttattatc gatatggttt tgcaatggta ttgttttaat
     4621 tactttggat tgattgataa cgtatatagt aattgattgt tgcaataatt gattaatttg
     4681 gtatccagca tttattagta agttttcaac tgtataagtt tgtttaatcg aatcattaaa
     4741 ttgaaataat aaatctgtac gataaagttg ctttttagta atggcgtatt ggaatttaat
     4801 ttgttttaat aaagcactcg ttaaaatact tgttaaaatg ctgacgataa atgtaatagg
     4861 atagtcaaag cggtatactt caaatgtata tctaggttcc gtaaaaaaat aattaaaaac
     4921 aaatacgtta ataattgctg ctaaaaagcc aatgatgtag gaccgcgtcc aaatggataa
     4981 caaaatgatg ccgatgagaa aaatcattaa aataatcgta ctagactcat gcttatcaag
     5041 ttgataaatc cacagtccca tcattacaca aattatttga atcagaatca tttttaacat
     5101 atctatggcg aaccgtttgc ctttaggacg atagggtgat ttgttcattt tcaattcaac
     5161 aggtatttgt ttgattggaa cgatttcgat tttatagcta tgttcaaagg acattaaatg
     5221 gtcaattaaa ggtttattga aaaagtctcg ccgcttattt ctaatatgtt gtccgataat
     5281 taatttggtt acatcttgat ttttacacca ttcgtctaat cctaatgcaa cggtttggct
     5341 ataaactact tttacttttg ctcctaaaga ttttgcaagc atgagatgtt gatgcacttg
     5401 cttttgacta tctttatatt gcctgctttt ttcgaataca tctatataaa tagcagtgaa
     5461 ttttgcatgt tctttttgag caatatggaa tgcctcttta attactgctt cattataaat
     5521 actcccacta attgccacag caatatgagg tttgagtgac gttttatgat tgtgtcggac
     5581 tttttcttta tcactcatca agtcggcaac tgttcttaac gtcaatgtac gcagttcgct
     5641 taggtgggca tacgtaaaga aattactaaa tgctacatct agcctatcct ttttatatac
     5701 cttgccagct tttaagcgtt taattaattg ttcaggtgag atatctacga cttctaatac
     5761 atcggcgctc attatgaaat agtcgggtac acgttcttta acatgtacac cggtcatcag
     5821 ttcaatttga ctacttaaac tttcaatatg ttgaatgttc aaagtagtat gaacatcgat
     5881 accatgattt aaaatttctt caatatccat atatcgtttc tcatgacgat ctctagaaat
     5941 gttcgtatgt gctaactcat cgataagtac tattgtcggc gattcttcga ttatgcggtc
     6001 gacatctaaa tattgaaaat gatgactacc atgtttagta aaattggttg cgattttagg
     6061 taattgttca gctaatgcat ttgtttcatc acgttgatgc ggttcaatat atcctagttt
     6121 aatatcaacg ttactttgaa atagttcaat ggcatttgaa agcatctcaa atgttttacc
     6181 aacacctgga ctgtaaccga tgtaaattgt taaagatcca cgcttttttc ctatgtttag
     6241 cgattcagtg tttgacataa ccttcacctc gatagcaata tcataatatt cttcacaatt
     6301 aaatgctata aaatttattt ctgcatacac atcttaatga tttcttaata actaagtttg
     6361 aaaattaatg tttctctcat aaacaagttg ttacgatgtt gttgtgttgt aaggaggtta
     6421 tggcaatgct catcgtttta ttattggtag ttattgcatt agttttgtat ttattttatg
     6481 ccttaattcg tagtgaaaaa ttttagaaat atattaagtg agggttatca tggaaataat
     6541 tttgtttcta acaatgatgg ttatgattgc atatgttttt agtggatatc tatatcgcgt
     6601 agcattggtg caatcgtcaa gagtagattt aatatttact agatttgaaa atatgtgttt
     6661 taaaattatc ggcacagatt tagaacacat gtcagctaaa acatatgtca aacattttct
     6721 ggcatttaat ggatttatgg gggttataac atttgtattg ctaatagttc agcaatggct
     6781 ttttttaaat ccaaatcata atttaaacca atcgatagat ttagcgttta atacagcaat
     6841 atctttttta acaaatagta atttacaaca ctataacggt gaatcaggtg tgacatattt
     6901 aacgcaaatg attgtaatga catatttaat gtttacatct agtgcatcag gttacgccgt
     6961 ttgtatagcg atgttgagac gtttaaccgg attgactaat atcattggta atttttatca
     7021 agatattgtt cggtttattg tccgagtgct tttaccatta tcatgtttaa ttagtatttt
     7081 attgatgact caaggtgtac cacaaacgtt gcatgctaat ttaatgattc ggactttaag
     7141 cggacatatt caacatattg catttggacc tattgcatca cttgaatcaa taaaacatct
     7201 tggtacgaat ggtggaggat ttttagcagg aaattctgca acaccttttg aaaatccaaa
     7261 tatttggagc aattttatag aaatgggcag tatgatgtta cttcctatgt caatgttgtt
     7321 cttatttggt cgcatgttaa gtagacatgg taaacgagta catcgtcatg cgctgatatt
     7381 atttgtcgca atgtttttca tttttatagc aattcttaca ttaactatgt ggagtgagta
     7441 tcgtggtaat ccaatactag caaatttagg aattcatgga gcgaatatgg aaggtaaaga
     7501 ggtacggttt ggagcaggtt tgtcagcact atttacagtt attacgacgg catttacaac
     7561 gggttctgtt aataacatgc atgatagctt aacgcctcta ggtggattag gaccaatggt
     7621 attaatgatg ctaaatgttg tatttggtgg cgaaggcgta ggactcatga atttattgat
     7681 atatgtctta ctgacagtgt ttatatgcag tttgatggtt ggtaaaacgc cagaatattt
     7741 aaatatgcca attggcgccc gtgaaatgaa atgtattgtc ttagtctttc tcatacatcc
     7801 aattttaatt ttagtatttt cagcacttgc ttttatgatt cctggagcaa gtgaaagtat
     7861 aacgaatccg tcttttcatg gtatctcaca agttatgtat gaaatgacat cagctgctgc
     7921 gaacaatgga tcagggtttg aaggactgaa agatgataca acattctgga atatctctac
     7981 aggaatcatt atgttgcttt ctcgttatat accaattatt ttgcaattaa tgatttcatc
     8041 aagtttagtg aataaaaaat cataccacca agataaacat acaatagcga ttgataaacc
     8101 ttattttggt gtatcactaa tcgtatttat cgttttacta agtggtttga catttattcc
     8161 agtactatta cttggtccta ttggtgaatt tttaacttta aaataaagta aagggatgga
     8221 aaaaatgcat catgtaaata aatatttcaa tcaaacaatg gtgatagaag ctttgaaaat
     8281 gagtttttat aagctgaatc ctaaacaatt aataaaaaat ccgataatgt ttgtcgtcga
     8341 ggttggaatg cttttaacgt tgatattaat atgttttcct gacatatttg gaactagcta
     8401 tctatcgcga ggttatttga ttacaatttt tatcatatta ttaataacta tattgtttgc
     8461 taatttttca gaagcctttg ctgaaggtcg tggaaaagcg caagctgata gtttaagaca
     8521 agcacaatca aatttaactg ctcgattgat agaagaaaat ggtgcgtatc ggatagtgaa
     8581 cgccacagag ttaaaagccg gacaaaatat tcgggtagaa aatggagaaa caatccctgc
     8641 tgatggcgtt gtaataaatg gattagcaac cgttgatgaa tctgcaatta ctggtgagtc
     8701 tgcacctgtg ataaaagaat caggtggcga ttttgatgga gtcattggcg gtacacttgt
     8761 aacatctgat tggttagaaa tacgtgttga gagtgaagcg ggtacatctt ttttagataa
     8821 aatgattgcc ttagttgaag gggcggaaag aaataaaaca ccaaatgaaa ttgcactttt
     8881 tacattatta acaacgttaa caattatatt tttagtcgtt attgtaacat tatatccaat
     8941 agcatcttac ctgcatttaa ttttacctat tgcgatgctc attgcgttaa cagtatgttt
     9001 aataccaacg acaatcggtg gtttgttatc ggcgattggt attgcaggca tggatcgtgt
     9061 tactcaattc aatgtattag cgaaaagtgg gcgtgctgta gaagtatgtg gagatgtcga
     9121 tgtaatgatt ttagacaaga caggcacaat cacttatggc aatcgaatcg ctagtgaatt
     9181 tttaccagtg aatcaacaaa tgatggaaaa attgatagtc gctgcttata tgtcatcaat
     9241 ttacgatgat acgccagaag gaaaaagtat tgtgcgatta gcgaagcaga tgtatataaa
     9301 tgagctgcct aaagacattg atggcacata taagcctttt acagctgaaa ctagaatgag
     9361 cggaatcatt acaaatgaaa tatcggtatt caaaggtgca cctaatagta tgataaatct
     9421 tgtcaaacaa caacaaggca atattcctct taatattgag tctatctgta tggatgtatc
     9481 gagtaaaggt ggcacaccat taatagtcat tgaaaacaac gtcatgcttg gcgtgattta
     9541 tttaaaagat gtgattaaag atggtcttgt agaacgattt gctgaattga gaaaaatggg
     9601 cattgaaaca gttatgtgta cgggcgataa tgctctaaca gcagctacaa ttgcaaaaga
     9661 agcgggtgtt gatagatttg ttgcagagtg taaacccgaa gataaaatta aagtcatcaa
     9721 agatgaacaa gcgaaaggcc atattgttgc gatgacagga gatggtacaa atgatgcacc
     9781 tgcgctagca caagcgaata taggattagc tatgaactca gggacaatta gtgctaaaga
     9841 ggcggccaat ctaattgatt tagattcgaa tcctacaaag ttaatagagg ttgtaaaaat
     9901 aggcaaacaa ctattaatga cgagaggtgc attaacgaca tttagtttgg ctaatgacgt
     9961 agcaaaatac tttgcaattt tacctgcact aatgatgtca acgattcctg aaatgacttc
    10021 actaaatatt atgcatttat cgtcacctaa atctgcaatt atatcagctt tgatattcaa
    10081 tgcattaatc attgttgcct taattccaat tgccatgaaa ggtgtaaaag taaagggtta
    10141 cagcattgat agaattttta taaataatat gttgatttat ggattaggtg gattaatcgt
    10201 cccgttttta ggcattaaat taatagatat gattgtacaa ttttttgtgt gaagaggaag
    10261 gagtctgtac catgaatacg ataagaaata gtatttgttt aacgataata actatggtat
    10321 tgtgtggatt tttattcccg ttagctatca cgttaattgg tcaaatattt ttttatcaac
    10381 aagcaaatgg tagtttaatt acgtatgata atcgtatagt tggatcaaaa ttgattggac
    10441 agcactggac agagacacga tattttcacg ggagaccgag tgcagttgat tataatatga
    10501 atcctgaaaa attatataaa aacggcgtat catcaggtgg aagtaatgaa tcgaatggaa
    10561 acaccgaatt aatagcacgc gtgaaacatc atgttaaatt tgataattca aatgtaacaa
    10621 tagatgctgc aacaagttct gggtcgggct tagatccgca tattacggtt gaaaatgcat
    10681 tgaagcaagc acctcgtatt gctgatgctc gtcatatatc tacttcaaga gtggccgact
    10741 tgattcaaca tcgtaagcag cgtggtgtgt taacaaatga ttatgtgaat gtgcttgaac
    10801 taaatattgc tcttgataag atgaaagatt gaatatagat gaaagacatc atgttgataa
    10861 aattataaca tggtgtcttt tttaggtatt caagtacttt ctgcttaact aaatttttag
    10921 atgtaccttt aggaagaata aattagcatg aaatattcca tatgctataa ttatcaacat
    10981 ataaaaataa aatgaaatag attataaatc cgtataattt ttatttgggg agaggggtat
    11041 atgtcagatt atactaagtt atctaagagt cctaagtcag cattgttgta ttactacatt
    11101 acgaatggct tagaatttat attgagtgta gctgtgtatg tcatttttta ttttatatgg
    11161 ttaagatttg aatggcctca gtacttaatt tatattttat ttgttttatg tactttgact
    11221 gttttaaaat taatcattaa gcctttatgg caatatcact gtcgctttta tcaagttgat
    11281 caactcagtg tacaatatcg tacatcattt tt
//
LOCUS       DAMZPP010000099        11267 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMZPP010000099 DAMZPP010000000
VERSION     DAMZPP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11267
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.099"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..795
                     /locus_tag="POH73_001956"
     CDS             <1..795
                     /locus_tag="POH73_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase II"
                     /protein_id="HDI6887891.1"
                     /translation="NNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIYQ
                     QLADSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPIVGMVGLIENVDYLNDFQ
                     PQVGDKLYVIGDTKDDFGGSQLEKLIYGKVNHEFESIDLSSEVKKGESIKTAIRQGVL
                     SHVQTVGKGGLLITLAKLSAHYGLGLKSLIDITNAQLFSETQGRYVVSVKSGKTLNID
                     NAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            774..2258
                     /locus_tag="POH73_001957"
     CDS             774..2258
                     /locus_tag="POH73_001957"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDI6887892.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPLLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKIKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKM
                     LAQESPVKADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHYFDSEGNLNV"
     gene            2251..3279
                     /locus_tag="POH73_001958"
     CDS             2251..3279
                     /locus_tag="POH73_001958"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDI6887893.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIETNEMYNIFNMGIGYTVIVDEKDAPRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            3282..3848
                     /gene="purN"
                     /locus_tag="POH73_001959"
     CDS             3282..3848
                     /gene="purN"
                     /locus_tag="POH73_001959"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDI6887894.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFGSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRQCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            3863..5341
                     /gene="purH"
                     /locus_tag="POH73_001960"
     CDS             3863..5341
                     /gene="purH"
                     /locus_tag="POH73_001960"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDI6887895.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPAAVAVKHMNPCGVGIGDTLEQAFHHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALNILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            5363..6610
                     /locus_tag="POH73_001961"
     CDS             5363..6610
                     /locus_tag="POH73_001961"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDI6887896.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQGILDFAKQQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCEFPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGELAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNEAIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVHDAQRDAYEK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(6878..7684)
                     /locus_tag="POH73_001962"
     CDS             complement(6878..7684)
                     /locus_tag="POH73_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDI6887897.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDVIFIIVLIAIIILAYYCSITLPITGIDDVRLGRVG"
     gene            complement(7677..9077)
                     /locus_tag="POH73_001963"
     CDS             complement(7677..9077)
                     /locus_tag="POH73_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6887898.1"
                     /translation="MLKVSDLRLKYPSGQRKVFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQVP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQHLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLENVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(9092..9667)
                     /locus_tag="POH73_001964"
     CDS             complement(9092..9667)
                     /locus_tag="POH73_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDI6887899.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(9744..9844)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(10251..10385)
                     /gene="graF"
                     /locus_tag="POH73_001965"
     CDS             complement(10251..10385)
                     /gene="graF"
                     /locus_tag="POH73_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HDI6887900.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     gene            10537..>11267
                     /locus_tag="POH73_001966"
     CDS             10537..>11267
                     /locus_tag="POH73_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887901.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHY
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVT"
BASE COUNT         3886 a         1667 c         2140 g         3574 t
ORIGIN      
        1 aacaatccat atgaaggtgg aaagatggta gtagctgaag cttatcgtaa tttaattgcc
       61 gtgggtgcaa caccattagc aatgactgac tgcttaaatt atggttctcc tgaaaagaaa
      121 gaaatttatc aacaattagc agattcaacg aaaggtatgg cagaagcttg cgacatactt
      181 aagacacctg ttgtttctgg aaacgtttct ttatacaacg aaacgaaagg cacttctatt
      241 ttccctacac caattgttgg tatggtaggt ctgattgaaa acgtagacta tttaaatgat
      301 tttcaacctc aagttggaga taagttatat gtaattggtg atactaagga cgactttggt
      361 ggtagtcaac ttgaaaaatt gatttatggc aaagtaaatc acgaatttga atctatagat
      421 ttgagttcag aagttaaaaa aggtgaatca atcaagaccg ctattcgtca aggggtatta
      481 tcacatgttc aaacggttgg taaaggtggc ttactgatta ccttagctaa actaagtgcg
      541 cattacggtt taggattaaa gtctttaata gatataacaa atgcacaatt gtttagtgag
      601 acacaaggcc gatatgttgt ttctgttaaa tcaggtaaaa ctttaaatat tgataatgca
      661 atagaaattg ggcttttaac agatagtgat aatttcaaag taacaacacc atatacagag
      721 attagtgaaa atgtttcaga tattaaacaa atatgggaag gggcaattgc tcaatgttta
      781 actactcagg attaaacgaa gaatgtggcg tgtttggtat ttggaatcat cctgaagcag
      841 cgcaactaac atatatggga cttcatagtt tgcaacatcg tggtcaagaa ggtgcaggta
      901 tagttgtttc tgatcaaaat gaattaaaag gcgagcgagg attaggctta ctaactgaag
      961 cgattaaaga tgaccaaatg gaacgattaa aaggatatca acatgcaatt ggtcacgtcc
     1021 gttatgctac ttcaggtaat aaaggtattg aaaatattca accgcttctg tatcactttt
     1081 atgatatgag tgtaggtatt tgtcataatg gtaacctcat taatgctaaa tcattacgtc
     1141 aaaatttaga aaaacaaggt gctatcttcc attcgtcttc agatacagaa gttattatgc
     1201 atttgattcg acgcagtaag gcgccaactt ttgaagaagc attaaaagaa agtcttcgaa
     1261 aaattaaagg tggttttaca tttgcaattt taacaaagga tgcgttatat ggcgcagtag
     1321 atccaaatgc tatcagacca cttgttgtag gtaaaatgaa agatgggaca tacatccttg
     1381 caagtgaaac atgtgcaatt gatgtgttag gtgcagaatt cgttcaagat attcatgcgg
     1441 gtgaatatgt cgtgattaat gataaaggta ttacagttaa atcttataca catcatacga
     1501 caactgcaat ttctgcgatg gaatatattt attttgcgag accagattca acgattgctg
     1561 gtaaaaatgt tcatgcagtg cgtaaggctt caggtaaaat gttagcacaa gaaagtccgg
     1621 taaaagctga tatggtcatc ggtgtaccta attcatcatt atctgcagcg agcggttatg
     1681 ctgaagaaat cggtttgcca tatgaaatgg gactagttaa aaatcaatat gttgcaagaa
     1741 catttattca accaacgcaa gagttacgtg aacaaggtgt aagagttaag ctatctgcag
     1801 tgaaagatat tgtagacggt aaaaatatta ttcttgttga tgattccatt gttcgaggta
     1861 cgacaattcg acgtattgtg aaaatgttaa aagattctgg tgcaaataaa gtgcatgtgc
     1921 gtatagcatc accggaattt atgttcccaa gtttttatgg aatcgatgtt tcaactacgg
     1981 cagaattaat ttctgcaagc aaatcacctg aagaaattaa agattatatt ggcgctgatt
     2041 cgttagcata tctatctgta gatgggttaa ttgaatcaat tggtttagat tatgacgcgc
     2101 catatagtgg cttatgtgta gaaagtttca ctggagatta tcctgcaggg ttatatgatt
     2161 atgaagcaaa ttataaagca catttaagtc atcgacaaaa gcaatatatt tctaaaaaca
     2221 aacactattt tgatagcgag ggaaatttaa atgtctaaag catatgaaca atctggtgta
     2281 aatattcatg ctggttatga agctgtagaa agaatgtcta gtcatgttaa acgtacgatg
     2341 cgtaaagaag ttatcggtgg tttaggtgga ttcggtgcta catttgattt atcacaatta
     2401 aatatgacag caccagtttt agtttctgga acagacggag taggtacgaa attaaaacta
     2461 gctatcgact atggtaaaca tgattcgata ggtatcgatg cagtcgcaat gtgtgttaat
     2521 gatattttaa cgacaggtgc agaaccatta tattttttag attacattgc tacaaacaaa
     2581 gttgttcctg aagttattga acaaattgtt aaaggtatta gtgatgcatg cgttgaaacg
     2641 aatactgcac ttatcggtgg agagactgct gaaatgggtg aaatgtatca cgaaggcgaa
     2701 tatgatgtag ctggatttgc tgttggagca gttgaaaagg atgactatgt agatggttca
     2761 gaagtgaaag agggacaagt tgttataggg cttgcgtcaa gtggcattca ttcaaatgga
     2821 tacagtttag tgcgcaaatt aattaatgaa tcaggcattg atttggcatc aaacttcgat
     2881 aatcgtccat ttatagatgt ctttttagaa ccaactaaat tatatgttaa acctgtactt
     2941 gctttgaaaa aagaagtttc tattaaggca atgaatcata ttactggtgg aggtttttat
     3001 gaaaatattc cacgtgcatt gccagccgga tatgctgcta gaattgatac tacatcattt
     3061 ccaacgccaa aaatatttga ttggttacaa caacaaggca atatagagac aaatgaaatg
     3121 tataacattt ttaacatggg tattggctat acggttatcg ttgatgaaaa agatgcacca
     3181 cgtgctttga agattttagc agaacaaaat gtggaagcct atcaaattgg tcatattgtg
     3241 aaaaatgagt caactgcaat tgaattgttg ggggtataac aatggttaaa attgcgattt
     3301 ttgcatcagg ttcaggaagt aactttgaaa atatagttga gcatgttgaa tcaggaaaac
     3361 ttgaaaatat tgaagttacg gcgctatata cggatcatca aaatgcgttt tgtatagata
     3421 gagcaaaaaa gcacgatatt cctgtttata ttaatgaacc aaaacaattt ggttcaaaag
     3481 cagcgtatga acaacattta gtaacattat taaatgaaga taaggtagag tggattattc
     3541 tagctggcta catgcgtcta attggtccag atttattagc ttcatttgaa ggtaaaatat
     3601 taaatataca tccatcacta ttgccgaaat ataaggggat tgacgcaata ggccaagcat
     3661 atcatagtgg cgatactatt actggttcga cagtacatta tgttgatagt ggtatggata
     3721 cgggagaaat tattgaacag agacaatgtg atattagacc ggacgattca aaagaacaat
     3781 tagaagagaa agtaaaaaaa ttggaatatg agttatatcc aagtgttatt gctaaaattg
     3841 taaaataagg agtatgtatg tgatgaagaa agctattttg agcgtatcaa ataaaacagg
     3901 tattgtagag tttgctaaag cgttaacgca attaaattat gaattatatt caactggtgg
     3961 tactaagcgt atattagatg aagcaaatgt accagttcgt tctgtttcag acttaacaca
     4021 ttttccagaa ataatggatg gccgtgttaa aacattacat ccagctgttc atggtggtat
     4081 tttggctgat cgaaataaac cgcagcattt aaatgaatta tcagaacaac atatagattt
     4141 aattgatatg gtagtagtta atttatatcc attccaacaa actgttgcta accctgatgt
     4201 gacgatggac gaagcaattg aaaatattga tattggtggt ccaacaatgt tacgtgctgc
     4261 agctaaaaac tataaacatg taacaacaat tgtacatcct gcagattatc aagaagtatt
     4321 gacgcgatta agaaacgatt cgttagatga gtcatataga caatcattaa tgatcaaagt
     4381 ttttgagcat actgcagaat atgatgaagc gattgtacgt ttctttaaag gggataaaga
     4441 aactttaaga tatggagaaa atccacaaca atcagcgtat tttgtgagaa cttcgaatgc
     4501 aaagcacacg attgcaggcg ctaaacaatt acatgggaaa caattaagct ataacaatat
     4561 taaagatgca gatgctacac tagctttagt taaaaagttt gatacacctg ctgcagttgc
     4621 agttaaacac atgaatccat gtggtgttgg tatcggtgac acgttagaac aagcatttca
     4681 tcatgcgtat gaagcggata gtcaatcaat atttggtgga attgttgcat taaaccgagc
     4741 tgtaacacct gagttagcag agcaattgca tagtatcttt ttggaagtca ttattgcacc
     4801 aaaatttaca gatgaagcat taaatatttt aaaacaaaag aagaatgtaa gattattaga
     4861 aattgatatg actatagaca gtaacgaaga agagtttgtt tcagtatctg gcggatattt
     4921 agttcaagat aaagacaatt atgtcgtgcc aaaagaagaa atgaaagttg ttacagaagt
     4981 tgcacctact gatgaacaat gggaagcaat gttattagga tggaaagttg taccatcagt
     5041 aaaaagtaat gcaattattt taagtaataa taaacaaact gtaggtatag gtgctggaca
     5101 aatgaatcgt gtcggtgctg ctaaaattgc gttagagaga gctattgaaa tcaatgatca
     5161 tgtagcgtta gtatctgatg gatttttccc tatgggagat acagttgaac ttgcagcaca
     5221 acatggtata aaggcaatta tccaaccggg tggttcgatt aaagatcaag attcaattga
     5281 tatggctaat aaacatggta ttgcaatggt agtcacaggc actcgacatt ttaaacacta
     5341 attttaaagg ggcgtaacat aaatgaatgt attagtaatt ggtgctggtg gacgagaaca
     5401 tgcacttgca tataaactta atcaatcgaa tctagttaaa caagtgtttg tcattccagg
     5461 taatgaggca atgacaccta tagctgaagt acacactgaa atttcagaat ctgatcatca
     5521 agggatacta gattttgcta aacagcaaaa tgttgattgg gtagttatag gtccagaaca
     5581 accgctaatt gatggattag cagacatttt acgagcgaat ggtttcaaag tgtttggtcc
     5641 aaataagcaa gcagctcaaa tcgaaggctc aaagttattt gctaaaaaga taatggaaaa
     5701 atataatatt ccaactgctg attataagga agtagagcga aaaaaggatg cattaacata
     5761 tattgaaaac tgtgaattcc ctgttgttgt caagaaagat gggttagctg ctgggaaagg
     5821 cgttattatt gcagatacaa ttgaagcagc cagaagtgct attgagatta tgtatggtga
     5881 tgaagaagaa ggtactgttg tgttcgaaac gtttttagaa ggtgaagagt tctcgctaat
     5941 gacatttgtt aatggtgaat tagcagttcc tttcgactgt attgcacaag atcataaacg
     6001 cgcatttgat catgatgaag gaccaaatac tggtggtatg ggggcttatt gtccagtacc
     6061 acatattagt gacgatgttt taaaacttac aaatgaaaca attgcacaac ccattgcaaa
     6121 ggcaatgctt aatgaaggtt atcaattctt cggtgtatta tacattggtg ctattctaac
     6181 taaagatggt ccaaaagtaa tagaatttaa tgcccgtttt ggtgatcctg aagctcaagt
     6241 attattaagt cgcatggaaa gtgatttaat gcagcatatt attgatttag atgaaggaaa
     6301 acgtactgaa ttcaaatgga aaaatgaagc tattgtaggg gtcatgttgg catcaaaagg
     6361 atatcctgat gcatatgaaa aagggcataa agtaagtggc tttgatttaa atgaaaacta
     6421 ttttgttagt ggattaaaga agcaaggtga tacctttgtt acttcaggtg gtagagttat
     6481 acttgccatc ggaaaaggtg acaatgtaca tgatgcacag cgagacgcat acgaaaaagt
     6541 atctcaaata caaagtgacc atttattcta tcgtcatgac attgcgaata aagcactaca
     6601 acttaaataa gtatatttaa aaatactaag attagctatg aacgaatcta taacgataga
     6661 tttgttcata gcttttttag ttgtagagtc taggacattg atttctgtac caaatttgtg
     6721 attatcatat gttgtaatac aaaagaggcg ccacaacatg tttggatgaa caaaataaca
     6781 tgtttgtggc acctcttttg tttagtatgg aataaatggt tttctttttc tatacaatga
     6841 atttctaatt tagtatctat acaattatgg ataaaattta acctacacga ccaagacgaa
     6901 catcatcaat gcccgtgatg ggtaaggtga ttgaacaata atatgccaaa ataataatgg
     6961 caattaaaac tataataaag attacatctt tatatgagaa aggtacgttg taatagtaag
     7021 tacgaggacc atctctaaat cctttcgact ccatcgcaac tgataattga tgtgcctttc
     7081 taatgttttg acttaataga ggtatgatta aatgcttaaa tcgctttaat cctctataat
     7141 ttgctgcgtc tatcatctga tagcgcattt ttaaagatct gcgaagctgt attaaagaac
     7201 taatcattaa aggtatcata cgaatggcag ccatgaatgc ataagcaact tttgatttaa
     7261 cctttaaatg ttgcattaaa ctataaaaaa tcatgacaac ctgagatgtg agtgcgatta
     7321 agataccgaa aaatgaaata gcaatagttc ttaatgatac atgtaaacca cgaactaaac
     7381 tttctgttgt aatatggata aatccgaatt tcaaaattgt atggctacca ttcccgtata
     7441 aaatcatgaa cagggaagag agtaatgcaa agccaatact tatagttata aaaattgctg
     7501 taattttaaa ctgagtacca ttaaacatca ataagaaaac taacattaag atagtgatat
     7561 aaagcataaa atcgaaatta tgcacaaata taataaagaa aaatagtata attccaagaa
     7621 atagtttcgt tataatgttg acatcatcaa caaatgattg ccgaaccttc cattgctcat
     7681 acattcgtat caccatcaca atctagtaac gcaccatctg aaattttaag tcttcttgat
     7741 gggtaacgtt caattatttc atcgtcatgt gtaaccatga caatactttg tcccaaatta
     7801 attcgttttt ggaaaagttt gatcaactgg aatgtattat ggctatcaag tccaaatgtc
     7861 ggttcatcta aaaagatgat atcagcttta gaacttagtg cggtagctac gctaaggcgt
     7921 cgtttttgac caatagacaa ctcataagga tgttgatctt taacattttc taaatctaaa
     7981 agttttaaaa gttgtatcgt ttcatcatca ctttgatctt tagaaaggtg attaaaatga
     8041 atgttaattt catcataaac cgaatttgtt ataaattgta actctgggtt ttgataaact
     8101 agatacatgt gttttgctgc atgtttaatt tttgttaaat gctgattttc aaaataaaca
     8161 tcaccttgat atttaatcaa ttgcataatt gattcaagca aggttgtttt accactacca
     8221 tttgcccctg taattgtaat ccactcacct agaccaattt ctaaatctga gaatgagagc
     8281 aacgttgatt taccgcgaat aatacgtcca tttttaaatt gtaataagtg tgagtttgtt
     8341 gttggaaagt caacacgact tggtgcgaat tcccatgcac gtggatgcca cacaccatat
     8401 tcactgagta aatgaacata cttctgtaat atgatttcag gacattcatc ggcaatgata
     8461 tttccgttat aatccatcaa aatgacgcgg tcgacatgat tccagatgtg tttaacttta
     8521 tgttcaacga ttacaacagt ttgatcttcc caaagttcaa ttagtttagt ccataaatct
     8581 tccgttgctt gaacatctaa cattgctgtc ggttcatcta aaaacaatgt ttttgattgt
     8641 tgaagaatgg tttcaacaat tgccaatttc tgtttcatcc cgccacttaa atctttgata
     8701 tacgtttcag gggtaacatt taaattgacc atatttaaag cattgataat taacgcatcc
     8761 atgtcttcac gtggtacttg tctattttct aaaacgaatg caagttcttc gtatactttt
     8821 ggcatacaaa actggctatc agggtcttgg aaaataacgc cacttaatgg gtcaacgatt
     8881 agttcatcat atttcatagg taattcaatt aaattaggaa caataccact taatacattc
     8941 agaagtgtac ttttaccgca accagaagga ccgagtaaaa gtactttttc tttgtcttga
     9001 atagtgatat ttaaatgatc gaaaacttta cgctgaccac ttggatattt taatcgtaaa
     9061 tcacttactt ttaacactct gaatgctcct cttataagtt gtcgtaatct tctttagcag
     9121 ctggcctaaa taattttgtt acgcctgtct tatctaaagc ttttactaaa aggtaagata
     9181 ggacgccggc gactactgca ccactaatta atctaaatac gatgaataat gttaagttcc
     9241 aacctgcaac ttcatttaaa taaccataga aataatcgat cgggaaagcc gcgatagctg
     9301 tacaaaaacc tgctaacata gctaccataa ctgaacgaga ttgatattta aaaattgcaa
     9361 agacaagttc acacgctaaa ccttgtatga aagcgtaaac gattgtcgga atatcgaaac
     9421 gacccataat gatagtttcg ccggcacctg cagcaaattc agccaataaa gcaatacctg
     9481 gttttggaat aattagatag cagacaatcg ctgccatgaa ccaaaccccg tttgttaatt
     9541 gttcgaggtg aaggcctgta gcttgcacac cattgtaaac aaaccaccat aaattgtaaa
     9601 taactgcgaa tactactgaa ataagtacgg ttactagaat ttcagatagc tttaaacctt
     9661 ttgacatttt tacatcctcc taataaaaaa acgcacaacc atccatagga aagttatgcg
     9721 ttcacaatat atattagtaa aacatatgta tagtaacact ttcctacgct agttcaagct
     9781 agatcaggtt caaagggttt gagggcaagc ctcatctcag tataaaacac ccctagtgtg
     9841 tgcgatttat ttaattaatt atactgtaag acgtttataa acttatgtca ataggttgtc
     9901 ttcatgaaat ttcgtttaat tcgatttaaa atttataata ttagcattag atttaaatta
     9961 aagatgtagt agaaatgtta gtaattaaag atataaaaat atgtgacatg taataatatt
    10021 aagttgataa gtgaagaggg atacttatca atcatacctc tttaacaaca gtgaacaacc
    10081 cgtgcataat ggcttgtgaa ttattgtttg taaggtaaaa gagggatacc agcggcgagc
    10141 gcaagcaaaa aagccccaaa caactaaaag ttataaggta aaagagggat acttgcgccg
    10201 agcgcatgca taaaatcccc taacaacgaa aagttgtaag gggatttaaa ttaatttagt
    10261 gtatcttgga tatcttgttt tgtttgatta atatcttctg ttttttcttc ttttttatct
    10321 tttagttttt cttcaacttc tttagctttt tctgctgctt ttttattttg attttcattt
    10381 gacatgatta attcctccca aattggataa ttatttatat ataaatctta cccggttgta
    10441 ctttcgttaa acttttctaa gtctatagca ctatttattc atttatctaa agacaacaac
    10501 attagattaa tatataatga ttttgaggtg aacataatgt cttttcttag gaaacacgcc
    10561 gaaattattt ttagctattt aatcggtatc gtttcactct tcactggtct cattatttta
    10621 attaacttgc ctttaattaa acaattaaat ggtggtaaaa aagttgatac acatgttcat
    10681 aatgtgtggg aatttctgaa tgcatttttc agtgaaatta ttaaagtaat gagtcgattt
    10741 ataggtaatt tccctatagt tagtgcaatt gtgataatta tattcggtat tttagttatg
    10801 ttgattggtc atacattact tagaactatt aagtatgact atgatatttc tatctttttc
    10861 ttagttatcg gtatcatgta ctttattatt acgcttatat taatgacgca agtatatgga
    10921 ttctttgcgg taattttcat tattccattt acaattcata ttggatatat cgtttataaa
    10981 gatgaattga atcaggaaaa tgttaaaaat cattacatgt ggattattgt aagttatggt
    11041 ataagttatt taattacaca aattgcattg tatggcagaa ttgatgctaa tgaaatagaa
    11101 tcaattgata tcttaagtgt caatgctttc tttataatta tgtggttact tggtcaaatg
    11161 gctatttgga atttcttgtt cttgcgccga gctttacctt taacaaagca agaattaggt
    11221 gaagaggagc cagaattatc aagaacaagt aaagggaatg tcacgaa
//
LOCUS       DAMZPP010000100        11233 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMZPP010000100 DAMZPP010000000
VERSION     DAMZPP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11233
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.100"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..186
                     /locus_tag="POH73_001967"
     CDS             <1..186
                     /locus_tag="POH73_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887902.1"
                     /translation="RIERLQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVK
                     IIEARTMSNLRGVESDV"
     gene            179..1756
                     /locus_tag="POH73_001968"
     CDS             179..1756
                     /locus_tag="POH73_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDI6887903.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFVIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQMIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSST
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIAAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKMKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITVVIIDLFVIKGYLYVKHAGFKMTDEQIT
                     IQNFVLLKRNIYYFKAQHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYASS
                     KDVQILKSWYLRGENDE"
     gene            1749..2240
                     /locus_tag="POH73_001969"
     CDS             1749..2240
                     /locus_tag="POH73_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDI6887904.1"
                     /translation="MSDYKFMSPHAKKVMRLSAVLFWIPIAFVLLIAFNILNWLFWEI
                     LDNYISIVSSVIIILLVAIFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHIENNK
                     NEE"
     gene            2244..2603
                     /locus_tag="POH73_001970"
     CDS             2244..2603
                     /locus_tag="POH73_001970"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDI6887905.1"
                     /translation="MIHGIGVDLIEIDRIKVLYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            2669..3817
                     /gene="alr"
                     /locus_tag="POH73_001971"
     CDS             2669..3817
                     /gene="alr"
                     /locus_tag="POH73_001971"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDI6887906.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYHRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVAAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            3902..4072
                     /gene="mazE"
                     /locus_tag="POH73_001972"
     CDS             3902..4072
                     /gene="mazE"
                     /locus_tag="POH73_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDI6887907.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            4069..4431
                     /locus_tag="POH73_001973"
     CDS             4069..4431
                     /locus_tag="POH73_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDI6887908.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVVHQKN"
     gene            4780..5781
                     /locus_tag="POH73_001974"
     CDS             4780..5781
                     /locus_tag="POH73_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HDI6887909.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN"
     gene            5900..6226
                     /locus_tag="POH73_001975"
     CDS             5900..6226
                     /locus_tag="POH73_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HDI6887910.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            6228..6707
                     /gene="rsbW"
                     /locus_tag="POH73_001976"
     CDS             6228..6707
                     /gene="rsbW"
                     /locus_tag="POH73_001976"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HDI6887911.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKEKNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            6682..7452
                     /gene="sigB"
                     /locus_tag="POH73_001977"
     CDS             6682..7452
                     /gene="sigB"
                     /locus_tag="POH73_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HDI6887912.1"
                     /translation="MAKESKSANEVSPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHK"
     gene            7887..10037
                     /locus_tag="POH73_001978"
     CDS             7887..10037
                     /locus_tag="POH73_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDI6887913.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVENAIRGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTAVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVAKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLKEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIHSIDKNKDKVSLTMIDPHE"
     gene            10030..10485
                     /locus_tag="POH73_001979"
     CDS             10030..10485
                     /locus_tag="POH73_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDI6887914.1"
                     /translation="MNNEILQRMVENLSEEKFGRTFQHRAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
     gene            11132..11214
                     /locus_tag="POH73_001980"
     tRNA            11132..11214
                     /locus_tag="POH73_001980"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11165..11167,aa:Leu,seq:gag)
BASE COUNT         4177 a         1454 c         2145 g         3457 t
ORIGIN      
        1 cgaattgaac gtttacaata cttgtcgatt aaatctaatc caattagtaa agtgcttaat
       61 ttgtataaag ttggatttat gactgcaggt catactattt atttaccgat gatgtctcat
      121 gacgatgtta aaataatcga agcgagaact atgtcgaatt tgagaggggt tgaaagtgat
      181 gtctgaacct caaaaattac atccgatttc atattttaat ggcataatca atgctatcaa
      241 acaaaacata gtcgtctttt ttattttcgt tatctttcaa ttgaaagatt ttgattatac
      301 gaatcccgaa tcatatttat ggataggtat tgtatttgta ttctttttaa tatcatacat
      361 ttctcaaatt gtcggaatta tgaacacgcg atattggatt gaggataatt atttcatttt
      421 aacaacaggt atttttaata aaaagcgtaa agaattaaat ataaaacgta ttcagtccgt
      481 tgatatgact caaggggtcg tcaatcaaat gataggtggc gtggatttac aaatcaaaac
      541 gccaagtgat ggtattgtct taagtgttat ttcgaaaaag caaggtgaat atcttgaacg
      601 atatattgat caattgcaaa cagagttgaa gacagaagtg gcgcaaaagt ctagtactga
      661 gaagaatgac aactgtaatc atgacattga aggtaatgtg gaagacatcg cagaaggtcg
      721 cttagatgaa gtgcagaaca ctgatagtat gctaaatcag aaaaataaac gcattcctat
      781 atatcagatg aattttaaag aatcactatt tatggcaatg accagtggcg ccattggtgt
      841 aacgttagca gctttagtac cgatttacgg cgctgtaaga gaattgatcc catggtcgaa
      901 gctaaatcat gaaattagtc aatgggttca aattgtaagt atttcagtat taattatcat
      961 tttactagta ttaatagcgg catatattat tggaacgatt ataacgatga taagatttta
     1021 tggcttttcg gtgatgcttg aaaatgagca acttaaaatt gaatatggac tctttaacaa
     1081 gatgaaagtg actgtaccaa cgaaacgatt gcaagctgtt gttgaacgtc agtcatatat
     1141 acgaagatta tttggctaca ctgcaataca ttttatcatc acaagtgatt ttgaaaataa
     1201 aaaaatcgaa gatgatgaca atgattcagg tagtgtgccg atattgccgt ttattaaacg
     1261 agacaaagca tatcaaatta tccagaatct agtaccagat ttatcttatc agcaagtcaa
     1321 tgaaggtatg cctttgtcag gtttccacag acacttttta atacctagta ttattgtcat
     1381 agctatggca ggaattggct gttacttttg gaatcaatta ttattgttat ttacgattat
     1441 aacagtagtc attattgatt tatttgtaat caaaggatat ctttatgtca aacatgcggg
     1501 ttttaaaatg acagatgaac aaattactat ccagaatttc gtgctattga aacgaaatat
     1561 ttattatttt aaagcgcaac acattatagg atttgacact tggcaacata cattacttaa
     1621 acgcaacaat ttaaataatt ttagttttat tgttgctaaa ggtgtgggag cccaatcgat
     1681 taacttaaaa tacgcaagta gcaaagatgt gcaaatattg aagtcgtggt atttgagagg
     1741 tgaaaatgat gagtgattat aagtttatgt caccacatgc taaaaaagtg atgaggttaa
     1801 gtgctgtgct gttttggata ccaatagcgt ttgtactttt aatagcattt aatattttga
     1861 actggctatt ttgggagata ttagataact atatatctat tgttagtagt gtgattataa
     1921 tattacttgt tgctattttc acgcttgtca tagcgccagt ctatcgatat aagcactgca
     1981 gatatgcatt tgaaaaacac catttgcgtg tacgaaacgg cattttattt ttggatgaaa
     2041 aagtaatacc atattttcgt attcaaaata ttgatatcga tgttggtcct ataatgagac
     2101 gctatcgatt agcaactttg acactttata cagcgggtgg tcaagctgaa attgaattaa
     2161 tagatagaag tgaagcacgt aggttgaaac gctggttaaa taacgagaga catatagaaa
     2221 acaataaaaa tgaggaataa tcaatgatac atggaattgg tgtagattta atcgaaatag
     2281 atcgaataaa agtgttatat agtaagcaac caaaattggt tgagcggatt ttaactaaaa
     2341 atgaacagca caaattcaac aatttcacac atgagcaacg taaaattgaa tttttagctg
     2401 gcaggtttgc tacaaaagaa gcgttcagta aagcattagg cacaggctta ggaaaacatg
     2461 tcgcatttaa tgatatagat tgttacaacg acgaacttgg caaaccaaag atcgattacg
     2521 aagggttcat cgtacatgtt agtatctcac acactgagca ttatgcgatg agccaagttg
     2581 ttttagaaaa gtcagcattt taaatttgtt atacaatgat aattaaaatc tagttttaaa
     2641 acgtgtggga attacaggag gaagtaatat gtcagataaa tattatagat ctgcgtatat
     2701 gaatgtagat ttaaacgctg ttgcatcaaa tttcaaagta ttcagtacat tgcatccaaa
     2761 taaaacagtg atggctgtcg ttaaagccaa tgcctatgga ctaggtagtg ttaaagtagc
     2821 acgtcattta atggaaaatg gtgccacatt ttttgctgta gcaacgttag atgaagcgat
     2881 agaacttaga atgcatggga ttactgctaa aattttagtc ttaggtgtgt taccagctaa
     2941 agatattgat aaagcgatac aacaccgagt tgccttaacg gttccgtcta aacagtggtt
     3001 gaaagaagca attaaaaaca tttctggtga gcaagagaaa aagttatggt tgcatattaa
     3061 attagataca ggaatgggac gtttaggtat taaagatact aaaacgtatc aagaagtgat
     3121 tgaaatcatt caacaatatg agcaacttgt atttgaaggc gtgtttacac actttgcctg
     3181 tgctgacgaa ccaggagata tgacaactga acaatatcac cgttttaaag atatggtcaa
     3241 tgaagcaatt aaacctgaat atatacattg tcagaactca gcaggttctc tattaatgga
     3301 ttgccaattc tgtaatgcaa taagaccagg aatttccctt tatggatatt atccatcaga
     3361 gtatgtacag caaaaagtta aagtacacct taaaccaagt gtgcaattaa ttgctaatgt
     3421 agttcaaaca aagacgctac aagcgggtga gtctgtaagt tatggtgcaa cttatacagc
     3481 tactgatcca acaacaatag cattgttacc tattggatat gcagatggct atttacgcat
     3541 tatgcaaggt agctttgtaa atgtaaatgg tcatcaatgc gaagttattg gtcgcgtatg
     3601 tatggatcag acaattgtaa aagtgccaga tcaagttaaa gctggagatt cggtgatttt
     3661 aatagataat catagagaaa gtccacagtc ggtagaggtg gcagctgaaa agcaacatac
     3721 tattaattat gaagtgcttt gtaacttgtc gagacgtttg ccgcgaatct atcatgatgg
     3781 tgatcaacgt tttgtaacaa atgaattgtt aaaataatat ggtcagtgca tataaaattt
     3841 gttatcatta gagtaattaa aggtcattta ataacttttg gaatcaattg gaggttctca
     3901 tatgttatct tttagtcaaa atagaagtca tagcttagaa caatctttaa aagaaggata
     3961 ttcacaaatg gctgatttaa atctctccct agcgaacgaa gcttttccga tagagtgtga
     4021 agcatgcgat tgcaacgaaa catatttatc ttctaattca acgaatgaat gattagacga
     4081 ggagatgttt atttagcaga tttatcacca gtacagggat ctgaacaagg gggagtcaga
     4141 cctgtagtca taattcaaaa tgatactggt aataaatata gtcctacagt tattgttgcg
     4201 gcaataactg gtaggattaa caaagcgaaa ataccgacac atgtagagat tgaaaagaaa
     4261 aagtataaat tggataaaga ctcagttata ttattagagc aaattcgtac acttgataaa
     4321 aaacgattga aagaaaaact gacatactta tccgatgata aaatgaaaga agtagataat
     4381 gcactaatga ttagtttagg gctgaatgca gtagttcatc agaaaaatta ggcgtctatt
     4441 atatgtattt ttcagagata aataaaatat tgatataaaa gacaataact ttataataat
     4501 tataactatt tctaaattct gtacgaagaa atttcttata aacaaagatt ttagcaaata
     4561 ccagttatga tattcatatt ttttattata aaaggatgtc ttaagttttt taggctttag
     4621 gtattccatc ctaaagtttt tttagcttaa aagtatcatc tacagcaaaa ttgcaaacga
     4681 caaaattgat aagtgcaatt aaataaatgt tagtaagtga atcataatta tccttgctta
     4741 agcatttgct ttgtaaggga agtgaggagg caactaatcg tggaagaatt taagcaacat
     4801 tataagggtt taattgatga aagtttaacg tgccaagata aagtagaatt gataaaaaag
     4861 tgtgagaaat acactgacga agtgattcgt aaggacgtct tgcctgaaga cattgtcgat
     4921 attcacaaaa actatatatt gacgttaaac ttaacgcgtg aagatgtgtt caagacatta
     4981 gatgtcttac aagaaatcgt taaaggcttt ggttatagtt atcgagatta tcaaagattg
     5041 gtagataaac ttcaagttca cgataaagag atagacttag cttctagctt acaacaaaca
     5101 atgcttaaaa cagatattcc acaatttgat agtattcaaa ttggcgttat ttcagtggcg
     5161 gcacaaaaag taagtggaga ttattttaat ttaattgacc ataacgatgg cacaatgagc
     5221 tttgctgttg cagatgtcat tggaaaaggt ataccagctg ctttagcaat gagtatgata
     5281 aagtttggca tggattctta tggacactca caattaccga gtgatggctt aaaacgttta
     5341 aatcgtgttg ttgaaaagaa tattaatcaa aatatgttcg tcacaatgtt ttatggttta
     5401 tatgaagaaa tgaaccattt attatattgt agttcagctg gtcatgagcc tggatatatt
     5461 tatcgcgctg aaaaagaaga gtttgaagaa atttcagtta gaggtagagt gttaggaatc
     5521 agttcacaaa cacgatatca acaacaagaa attccaatat accttgatga tttaattatc
     5581 attttaacgg atggtgtgac tgaagctaga aatagtgaag gtacctttat agataaacaa
     5641 aaacttttag aatatattaa aaaacataaa catatgcacc cacaagatat tgttcaaatt
     5701 atctatgaag caattttaaa acttcaaaac ccaaataaaa aagatgatat gactattttg
     5761 attataaaaa gagtaaatta atttaaaaaa gaagattaga aattatttcg atgggtatat
     5821 aataatttga aatataaata tggtggatac agcgcttaaa atgaagataa atatttttaa
     5881 taagtaggag tgtaatgaaa tgaatcttaa tatagaaaca accactcaag ataaatttta
     5941 cgaagttaaa gtcggtggag aattagatgt ttatactgtg cctgaattag aagaggtttt
     6001 aacacctatg agacaagatg gaactcgtga tatttatgtt aatttagaaa atgtgagtta
     6061 tatggattcg acaggtttag gtttattcgt aggtacatta aaagcattaa accaaaatga
     6121 taaagagcta tacattttag gtgtgtcaga tcgtatcggt agactatttg aaattactgg
     6181 tcttaaggat ttaatgcatg ttaatgaagg aacggaggtc gaataacatg caatctaaag
     6241 aagattttat cgaaatgcgc gtgccagcat cggcagagta tgtaagttta attcgtttaa
     6301 cactttctgg cgttttttcg agagctggtg ctacatatga tgatattgaa gatgccaaga
     6361 ttgcagttag tgaagctgtg acaaatgcag ttaaacatgc atacaaagaa aaaaacaatg
     6421 tgggcattat taacatatat tttgaaattt tagaagataa aattaaaatt gttatttctg
     6481 ataaaggtga cagttttgat tatgaaacaa ctaaatcaaa aataggtcct tacgataaag
     6541 atgaaaatat agacttttta cgtgaaggtg gcctaggttt atttttaatc gaatctttaa
     6601 tggatgaagt gacagtatat aaagaatctg gtgtgacaat cagtatgact aagtatataa
     6661 aaaaagagca ggtgcgaaat aatggcgaaa gagtcgaaat cagctaatga agtttcacct
     6721 gagcaaatta accaatggat taaagaacac caagaaaata agaatacaga tgcacaagat
     6781 aagttagtta aacattacca aaaactaatt gagtcattgg catataaata ttctaaagga
     6841 caatcacatc acgaagattt agttcaagtt ggtatggttg gtttaatagg tgccataaat
     6901 agattcgata tgtcctttga acggaagttt gaagcctttt tagtacctac tgtaatcggt
     6961 gaaatcaaaa gatatctacg agataaaact tggagtgtac atgttccgag acgtattaaa
     7021 gaaattggac caagaatcaa aaaagtgagc gatgaactaa ccgctgaatt agagcgttca
     7081 ccttctatca gtgaaatagc tgatcgatta gaagtttcag aagaagaagt gttagaagca
     7141 atggaaatgg gacaaagtta taatgcgtta agtgttgacc attccattga agctgataaa
     7201 gatggttcaa ctgttacgct attagatatt atgggacaac aagatgacca ttatgactta
     7261 actgaaaaac gtatgatttt agaaaaaata ttacctatat tatctgatcg cgaacgagaa
     7321 atcatacaat gtacgtttat tgagggtttg agtcaaaaag agacaggtga gcgtatcggt
     7381 ttaagtcaaa tgcatgtttc cagacttcaa agaacggcaa ttaagaaatt acaagaagca
     7441 gcacataaat agaatttgct tattaataat gcgttttata atgaaaaatc catataatta
     7501 tcccttgatt attaaattga aatcgaggga tatttttaat ttaattaaga ttttcgaatt
     7561 aatacattat taacgtagtt taatgtgtat ccacataaat gtcgcgatat agtattaata
     7621 atttaagtga agaagatatc taattgtcag tttaattaag tggattgcta ttagaataaa
     7681 aaaagtaggt ttagattatg aaatttagga aatgatggtg tgtcattttc aataatctta
     7741 gtgcgtttta agatatagta tgacctaata attcgttttt aaagttttgg aagttaaaat
     7801 tgcattaagt atcatacctt aatagaagta ttttagaata tgttaaaata aatgagtaaa
     7861 tttaagaaaa agtgtgggtt aagtaaatgg acaatcaatt gattaattca atcatagaga
     7921 aatatcaatt tagtaaaaaa caaattgaag cagtgttaac actgcttgaa gaaaaaaata
     7981 cagtaccatt tattgcgagg tatcgaaaag agcaaactgg tggactagat gaagttcaaa
     8041 taaagcaaat tgatgacgaa taccaatata tggtcaattt acaaaaacgt aaagaagaag
     8101 ttatcaaaaa tatagaacag caaggattac ttactgagga attaaagaaa gatattttaa
     8161 aacagaacaa attacaacgt gttgaagacc tatatagacc ttttaaacaa aagaaaaaga
     8221 ctagggcgac tgaggcgaaa cgtaaaggat tagaaccatt agcgatatgg atgaaggcac
     8281 gtaaacatga agtatcaatt gaagaaaaag cacaacaatt tataaatgaa gaagtgcaat
     8341 cggttgaaaa tgctatcaga ggtgcgcaag atattattgc tgaacaaatt tcagataatc
     8401 ctaaatatag aacaaaaatt ttaaaagata tgtatcatca aggtgtgtta actacatcta
     8461 aaaagaaaaa tgctgaagat gaaaaaggta tttttgaaat gtactatgca tatagtgagc
     8521 caattaaacg cattgctaat catagagttt tagctgtgaa tcgtggcgaa aaagagaaag
     8581 tattatctgt aaagtttgaa ttcgatacga cagcagtaga ggacttcatt gcacgtcaag
     8641 aaatcaatca taataatgta aatcgcagtt atattttaga ggcgattaaa gatagcttga
     8701 aacgcttaat tgtcccttcg atagagcgtg aaatccatgc tgatttaact gaaaaagctg
     8761 aaaatcatgc aatagatgtt tttagtgaaa acttaagaaa tctattactg caacctccaa
     8821 tgaaaggtaa acaaatatta ggcgtagatc ctgcatttag aacaggttgt aaattagcag
     8881 tcattaaccc atttggtact tttatagcaa aaggtgtgat ttatccgcat ccaccagttg
     8941 ctaaaaaaga agcagcagag aaggattttg tacaaatggt taaagcgtat gatgtgcaat
     9001 taattgcaat tggcaatggt acagcaagtc gtgaaacaga acaatttgtt gcagatttaa
     9061 ttaaaaagca tcagttgcca gtacaattca tcattgtcaa tgaagcgggc gcctcagtat
     9121 actcagcatc agaaattgct agagatgaat ttcctgattt tcaagtggaa gaaagaagtg
     9181 ccgtatcaat tggacgacgc gttcaagatc cattaagtga attagttaaa atcgatccta
     9241 aatctattgg tgttggacaa taccagcatg atgttaatca aaaagcacta gaaaatgcat
     9301 taactttcgt tgttgaaaca gcagttaacc aagtaggtgt tgatgtcaat acagcatcat
     9361 catcattatt acaatatgtg tctggtctaa gttcgcaaat tgctaaaaat ataattgcat
     9421 atagagaaga aaatggcgca ataaagcata ataaagagtt aagcaagatt aagcgtttgg
     9481 gtgctaaaac ttttgagcag agtataggat ttttaagaat agtagacggg tcagagccat
     9541 tggataatac atcgattcat cccgaaagct ataaagtaac gtatcagtta ttagataaac
     9601 tcggatttgg tggtaatgat ttaggtagtg atgcattaaa agctaaatta aattcattgg
     9661 atatggatga actggcgatt gaattacaag tcggtgtacc tacattagag gatattatta
     9721 aatccttgaa agcacctaat cgagatccaa gggacgagtt tgaaacacca atccttaaat
     9781 ccgatgtgct atcaattgaa gatttaaaag aaggaatgaa attgagtggc acagttagaa
     9841 atgtagtaga ttttggagca tttgtagata taggtgttaa acaagatgga cttgtccatg
     9901 tttcgaaact ttctaagaaa tttgttaaaa acccaatgga tattgtcagt gttggtgata
     9961 tcgtagacgt ttggattcat agtattgata aaaataaaga caaagtatca ttaacaatga
    10021 ttgatccaca tgaataacga aatacttcag cgaatggtcg agaacctttc ggaagaaaaa
    10081 tttggacgta cctttcaaca tcgtgcatat tttaataaaa ggttacgtac aacaggcgga
    10141 cgttatcttt taaagtctca tgatattgaa attaatccaa aacaatatga gcattatgga
    10201 gaagatgctg ttgtaaaaat tatactgcat gaactgtgtc attatcactt gcatattgct
    10261 ggtaaaggtt atcaacataa agatcaagat tttaaacgat tgagccaaca ggttggagcg
    10321 cctagatttt gtaacagcat tgaaagctat caacaacggg caaattatga atattattgc
    10381 actaaatgtc atgcaaaata tatacgtatc cgtaaagttg atacgaatcg tatgcgttgc
    10441 ggacattgca atggaaaatt aagaatgaaa agacaattga aataagtaca atacaaatag
    10501 agctaattca tgtatatgta agattattta tgagttgtat ggaatttata tagaatttat
    10561 cagtgagtta ttttgattgg taaacatagt aattgaataa gaagaaattg tgtttataaa
    10621 atgtttaaag ccgagattgt cgtaatttaa tacttcgcaa tcgcggcttt tctactttta
    10681 atataggtaa tggcataaat tgtgacttga taggatgaaa ttaaatagat tagattaaaa
    10741 atcttggcgt attttagtaa tgaattatat ataaacttta cccaattaaa gctttaaagt
    10801 ggcacaaaag ataattatac atttttaatg acattttgat tgaatttatc tattgactgt
    10861 acagcttata gttagtatga ttaaacacgt tccttaaaaa agaacaaatt aaataaactt
    10921 tgatttgaaa gacagtcaat tctaaattta atttataaaa tatgttgaca ataagttctt
    10981 aatgttttat tatgaataac gttgtgttaa caactgataa cacaaattaa tttaatgtaa
    11041 aaaaacgatt aaaagttatt gacaaatatt tttaatcata atataataaa taacgttctg
    11101 aaaaacgaag atgttttaaa tgcctatatc cgcggtcgtg gcggaatggc agacgcgcta
    11161 ggttgagggc ctagtgggag aaatcccgtg gaggttcaag tcctctcggc cgcatcaaaa
    11221 ttcttaatta aat
//
LOCUS       DAMZPP010000101        11169 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMZPP010000101 DAMZPP010000000
VERSION     DAMZPP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11169
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.101"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..1512
                     /gene="ilvD"
                     /locus_tag="POH73_001981"
     CDS             <1..1512
                     /gene="ilvD"
                     /locus_tag="POH73_001981"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDI6887915.1"
                     /translation="KEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADAA
                     ETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTLS
                     SMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNGT
                     ALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLHT
                     LAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKDG
                     TLHPDRITVTGKTLRENNEGKEIKNFDVIHSLDAPYDAQGGLSILFGNIAPKGAVIKV
                     GGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAPT
                     SSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTNR
                     TLKVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            1540..3309
                     /gene="ilvB"
                     /locus_tag="POH73_001982"
     CDS             1540..3309
                     /gene="ilvB"
                     /locus_tag="POH73_001982"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDI6887916.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVKTIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDTAFAYQGPALIEVR
                     ISPTEAVNPMVPSGKSNHEMEGL"
     gene            3309..3563
                     /locus_tag="POH73_001983"
     CDS             3309..3563
                     /locus_tag="POH73_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDI6887917.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            3685..4704
                     /gene="ilvC"
                     /locus_tag="POH73_001984"
     CDS             3685..4704
                     /gene="ilvC"
                     /locus_tag="POH73_001984"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDI6887918.1"
                     /translation="MEEFIMTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDN
                     GYDVVIGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNL
                     EKHNALAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQD
                     ASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGSVSKLIQSGFETL
                     VEAGYQPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVK
                     ENMKAVLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKS
                     KSIEK"
     gene            4734..6263
                     /locus_tag="POH73_001985"
     CDS             4734..6263
                     /locus_tag="POH73_001985"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDI6887919.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYIRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVIKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6266..7312
                     /gene="leuB"
                     /locus_tag="POH73_001986"
     CDS             6266..7312
                     /gene="leuB"
                     /locus_tag="POH73_001986"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDI6887920.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFAN
                     IRPTTVVKGASSLSPLKAERVEGTDLVIVRELTSGIYFGEPRYFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7326..8696
                     /gene="leuC"
                     /locus_tag="POH73_001987"
     CDS             7326..8696
                     /gene="leuC"
                     /locus_tag="POH73_001987"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDI6887921.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSNDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            8697..9269
                     /gene="leuD"
                     /locus_tag="POH73_001988"
     CDS             8697..9269
                     /gene="leuD"
                     /locus_tag="POH73_001988"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDI6887922.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKNAREHLAKYVEIEVDLPNQTVSSPDKSFHFEIDE
                     TWKNKLVNGLDNIAITLHYESLIEKYEKSL"
     gene            9284..10552
                     /gene="ilvA"
                     /locus_tag="POH73_001989"
     CDS             9284..10552
                     /gene="ilvA"
                     /locus_tag="POH73_001989"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HDI6887923.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKSYSPNTKIIGVEPSGA
                     SSMYESVVVNNQVITLPNIDKFVDGASVARVGDITFEIAKKNVDNYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDYD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
     gene            complement(10629..11045)
                     /locus_tag="POH73_001990"
     CDS             complement(10629..11045)
                     /locus_tag="POH73_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887924.1"
                     /translation="MKIFIGTMLTFLFVCSLIVCGFFAYKIYFPTDAENKSEIPNHMQ
                     ENKHEKENTHNINNNGSEANENFNNNKNRIENIEDKQKAYALMSIPRHNLPESEKAIY
                     DKVISTAIQNINNGVGNEEDFRLKEIYDGIQSVQPQ"
BASE COUNT         3779 a         1851 c         2122 g         3417 t
ORIGIN      
        1 aaagaagcaa ttagagaagc cggtgccatt ccatttgaat tcaatacaat tggtgttgat
       61 gatggaatag ctatgggaca tatcggaatg cgatattctc taccatcacg tgaaattatt
      121 gcagatgcag ctgaaactgt aattaacgct cattggtttg acggcgtatt ttacattcct
      181 aattgtgaca agattacacc cggtatgatt ttagcagcca tgaggacaaa cgtaccagct
      241 atcttttgct ctggtggacc aatgaaagct ggcttatctg cacatggtaa agcattaaca
      301 ctttcatcaa tgtttgaagc agtcggcgct tttaaagaag gatcgatttc taaagaagaa
      361 tttttagata tggaacaaaa tgcctgccct acttgtggtt catgtgctgg gatgtttact
      421 gcaaattcaa tgaactgttt gatggaagtt ttaggtctag cattaccata caacggtact
      481 gcacttgcag tcagtgatca gcgacgcgaa atgattcgcc aagcagcttt taaattagtt
      541 gaaaatatta aaaatgattt aaaaccacgt gatatcgtta ctcgcgaagc aattgatgat
      601 gcatttgcac ttgatatggc tatgggtggt tcaacaaaca cggtactgca tacgttagcc
      661 attgccaatg aagctggtat tgattatgac ttagagcgca ttaatgctat tgccaaacgc
      721 acgccatatt tatcaaaaat agcacctagt tcatcgtatt caatgcatga tgtgcatgaa
      781 gctggtggcg tcccagcaat tattaatgaa ttgatgaaga aagatggcac gttacaccca
      841 gatagaatca cagttactgg caaaacgtta cgtgaaaata acgaaggcaa agaaattaag
      901 aactttgatg tcattcactc tcttgatgca ccatatgatg cacaaggcgg tttatctatc
      961 ttatttggta atatcgcccc taaaggcgca gttattaaag ttggcggcgt tgatccatct
     1021 atcaaaacat ttactgggaa agcaatttgt ttcaattcgc atgatgaagc tgttgaagca
     1081 atagacaatc gtaccgttcg tgcaggccac gtcgttgtca ttagatatga aggacctaaa
     1141 ggtggaccag gtatgcctga aatgttagca cctacttcct ctattgttgg tcgtggctta
     1201 ggtaaagatg ttgcattaat tactgatggt cgtttttccg gtgccacaag aggtattgca
     1261 gttggtcata tttcccctga agctgcatct ggtggaccaa ttgccttaat tgaagatgga
     1321 gatgagatta ctattgattt aacaaatcgt acattaaagg taaaccagcc tgaagatgtt
     1381 ctggcgcgtc gccgagaatc tttaacacca tttaaagcga aagtaaaaac aggttatcta
     1441 gctcgttata ctgccctagt aactagcgca aatacaggtg gcgtcatgca agtccctgag
     1501 aatttaattt aatttatttt tatattggag atggttaaaa tgtctaaaac tcaacatgaa
     1561 gtaaaccaaa atattgaccc tttaaaaatg gctgaatcac ttgaacctga acaactaaat
     1621 gaaaaaactt taaatgacat gcgttcagga tcagaagtgc tagtagaagc tctacttaaa
     1681 gaaaatgtgg attatttatt cggttatcct ggtggtgccg tactaccttt atacgacaca
     1741 ttttatgatg gtaaaatcaa acatatttta gcaagacacg aacaaggtgc tgttcatgct
     1801 gcagaaggtt atgcacgtgt atctggtaaa acaggcgtcg ttgtagttac aagcggtcca
     1861 ggtgcaacta atgtaatgac aggtattacg gatgcacatt gcgactcttt accactagtt
     1921 gtattcactg gacaagttgc tacaccaggc attggtaaag atgcattcca agaagcggat
     1981 attctatcta tgacttcacc aattacaaaa caaaattatc aagtgaaacg tgttgaagat
     2041 atccctaaaa tcgtacacga agctttccat gtagctaatt ctggacgcaa aggtcctgta
     2101 gtgattgatt ttccaaaaga tatgggtgtt ttagctacaa atgtggattt atgcgacgaa
     2161 atcaatattc caggttatga agttgttaca gaaccagaaa ataaagacat tgacactttc
     2221 atctcacttt taaaagaagc gaaaaagcct gtcgtattag ccggcgcagg tattaatcaa
     2281 tcaaaatcaa atcaattatt aacacagttt gttaataaac atcaaatccc aacagttact
     2341 actttactag gtctaggtgc tgtaccttat gaggatacac tatttttagg tatgggaggt
     2401 atgcatggtt cttatgctag taacatggca ttaactgagt gtgatttact cattaattta
     2461 ggtagccgct tcgatgatag attagcaagc aaacctgatg cctttgcacc taacgccaaa
     2521 attgtacatg tagatattga tccttcagaa atcaataaag ttattcatgt agatttaggt
     2581 attattgcag actgtaaaag atttttagaa tgtttaaatg ataaaaatgt taagactata
     2641 gaacacagtg actgggttaa acattgtcaa aataataagc agaaacatcc atttaaactt
     2701 ggtgaagaag atcaagtatt ttgtaagcca caacaaacaa tcgaatatat cggcaaaatt
     2761 acaaatggtg aagcaattgt tactacagac gtgggacaac atcaaatgtg ggcagctcaa
     2821 ttttatccat ttaaaaatca cggacaatgg gttacaagcg gtggtttagg aacaatggga
     2881 ttcggtattc cttcgtcaat tggtgccaaa ttagctaatc ctgataaaac agtcgtatgc
     2941 ttcgtcggtg acggtggttt ccaaatgaca aaccaagaaa tggcactatt acccgaatat
     3001 ggtttagatg tcaaaatcgt actaatcaat aatggaacat taggtatggt taaacaatgg
     3061 caagataagt tctttaatca acgcttctca cactcagtat ttaatggtca acctgatttt
     3121 atgaaaatgg cagaagcata tggcgtcaaa ggtttcttaa tcgataagcc agaacaactg
     3181 gaagaacaat tagatacagc gtttgcttat caagggccag ctttaattga ggttcgtatt
     3241 tcccctactg aagctgtaaa cccaatggtt ccaagtggca aatcaaatca tgaaatggag
     3301 ggcttataat gacaagaatt cttaaattac aagttgcgga tcaagtcagc acgctaaatc
     3361 gaattacaag tgcttttgtt cgcctacaat ataatatcga tacattacat gttacacact
     3421 ctgaacaacc tgggatttct aacatggaaa ttcaagtcga tattcaagat gatacatcac
     3481 ttcatatttt aattaaaaaa ttaaaacaac aaattaatgt tttaacggtt gaatgctacg
     3541 accttgttga taacgaagct taattttaag acaaaggcaa tgatgcgcta attagttata
     3601 gatatatcat aggctgctag ttaacatctg ccactattac aaagctatat ttcagaattt
     3661 tcgaaaaaca aaacatttaa ttatttggag gaatttatta tgacaacagt ttattatgat
     3721 caagatgtaa aaacggacgc tttacaaggc aaaaaaattg cagtagtagg ttatggatca
     3781 caaggccacg cgcatgcaca aaacttaaaa gacaatggat atgatgtagt catcggtatt
     3841 cgcccaggtc gctcttttga caaagctaaa gaagatggat ttgatgtgtt ccctgttgca
     3901 gaagcagtta agcaagctga tgtaattatg gtgctattac ctgatgaaat tcaaggtgat
     3961 gtatacaaaa acgaaattga accaaattta gaaaaacata atgcgcttgc atttgctcat
     4021 ggctttaaca ttcattttgg tgttattcaa ccaccagctg atgttgatgt atttttagta
     4081 gctcctaaag gaccgggtca tttagttaga cgtacatttg ttgaaggttc tgctgtacca
     4141 tcactatttg gtattcaaca agacgcttca ggtcaagcac gtaatattgc tttaagttac
     4201 gcaaaaggta ttggtgcaac tcgtgcaggt gttattgaaa caacatttaa agaagaaact
     4261 gagacagatt tatttggtga acaagcagta ctttgcggta gtgtatcgaa attaattcaa
     4321 agtggctttg aaacattagt agaagcaggt tatcaaccag aattagctta ttttgaagta
     4381 ttacatgaaa tgaaattaat cgttgatttg atgtatgaag gcggtatgga aaatgtacgt
     4441 tactcaattt caaatactgc tgaatttggt gactatgttt caggaccacg tgttatcaca
     4501 ccagatgtta aagaaaatat gaaagctgta ttaactgata tccaaaatgg taacttcagt
     4561 aatcgcttta tcgaagacaa taaaaatgga ttcaaagaat tttataaatt acgcgaagaa
     4621 caacatggtc atcaaattga aaaagttggt cgtgaattac gcgaaatgat gccttttata
     4681 aaatctaaaa gcattgaaaa ataagataga cctacaatga ggagttgtta aatatgagta
     4741 gtcatattca aatttttgat acgacactaa gagacggtga acaaacacca ggagtgaatt
     4801 ttacttttga tgaacgcttg cgtattgcat tgcaattaga aaaatggggt gtagatgtta
     4861 ttgaagctgg atttcctgct tcaagtacag gtagctttaa atctgttcaa gcaattgcac
     4921 aaacattaac aacaacggct gtatgtggtt tagctagatg taaaaaatct gacatcgatg
     4981 ctgtatatga agcaacaaaa gatgcagcga agccggtcgt gcatgttttt atagcaacat
     5041 cacctattca tcttgaacat aaacttaaaa tgtctcaaga agacgtttta gcatctatta
     5101 aagaacatgt cacatacgcg aaacaattat ttgatgttgt tcaattttca cctgaagatg
     5161 caacgcgtac tgaattacca ttcttagtga aatgtgtaca aactgcagtt gacgctggag
     5221 ctacagttat taatattcct gatacagtcg gctacagtta ccatgatgaa tatgcacata
     5281 ttttcaaaac cttaacagaa tctgtaacat cttcaaatga aattatttat agtgctcatt
     5341 gccatgacga tttaggaatg gctgtttcaa atagtttagc tgcgattgaa ggcggtgcga
     5401 gacgaattga aggcactgta aatggtattg gtgaacgagc aggtaatgca gcacttgaag
     5461 aagtcgcgct tgcactatac attcgaaatg atcattatgg tgctcaaact gcccttaatc
     5521 tcgaagaaac taaaaaaaca tcggatttaa tttcaagata tgcaggtatt cgagtgccta
     5581 gaaataaagc aattgttggc caaaatgcat ttagtcatga atcaggtatt caccaagatg
     5641 gcgtattaaa acatcgtgaa acatatgaaa ttatgacacc tcaacttgtt ggtgtaagca
     5701 cgactgaact tccattagga aaattatctg gtaaacacgc cttctcagag aagttaaaag
     5761 cattaggtta tgacattgat aaagaagcgc aaatagattt atttaaacaa ttcaaggcca
     5821 ttgcggacaa aaagaaatct gtttcagata gagatattca tgcgattatt caaggttctg
     5881 agcatgagca tcaagcactt tataaattgg aaacactgca actacaatat gtctctagcg
     5941 gccttcaaag tgctgttgtt gttattaaag ataaagaggg tcatatttac caggattcaa
     6001 gtattggtac tggttcaatc gtagcaattt acaatgcagt tgatcgtatt ttccaaaaag
     6061 aaacagaatt aattgattat cgtattaatt ctgtcactga aggtactgat gcccaagcag
     6121 aagtacatgt aaatttattg attgaaggta agactgtcaa tggctttggt attgatcatg
     6181 atattttaca agcctcatgt aaggcatacg tagaagcaca tgctaaattt gcagctgaaa
     6241 atgttgaaaa ggtaggtaat taattatgac ttataacatt gttgccctac ctggtgatgg
     6301 aatcggtcca gaaattttga acggatctct atcattgctt gaaattataa gtaataaata
     6361 taattttaat tatcaaatag agcaccacga atttggtggt gcctctattg atacattcgg
     6421 cgagccttta actgagaaaa ccttaaatgc gtgtaaaaga gcagatgcta ttttactggg
     6481 tgcaatcggt ggacctaaat ggacagatcc taacaatcga ccagaacaag gattattaaa
     6541 attacgtaaa tcattaaatt tatttgcaaa tatacgtccc actaccgttg tcaaaggcgc
     6601 tagttcttta tcacctttaa aggcagaacg cgttgaaggc acagatttag ttatagtccg
     6661 tgaattgaca agtggtattt attttggaga acctagatat tttaataatc acgaggcctt
     6721 agattctctt acttatacaa gagaagaaat agaacgcatt gttcacgtag catttaaatt
     6781 ggccgcttca agacgaggaa aactaacatc agttgataaa gaaaatgtat tagcttctag
     6841 taaattgtgg cgcaaagtcg taaatgaagt gagtcaatta tatccagaag taacagtgaa
     6901 tcacttactt gttgatgctt gtagtatgca tttaatcaca aatccaaaac aatttgacgt
     6961 catcgtttgc gaaaacttat ttggcgatat tttaagtgat gaagcttcag tgattcctgg
     7021 ttcacttggt ttatcacctt ctgctagttt tagtaacgat ggtccaagat tgtatgagcc
     7081 tattcatgga tcagcaccag atattgcagg taaaaacgtt gccaatccat ttggaatgat
     7141 tctatcttta gcgatgtgtt tacgtgaaag cttaaatcaa ccagatgctg cagatgaatt
     7201 agaacaacat atttataaca tgatagaaca tgggcaaacg acagcagatt taggcggcaa
     7261 actgaatact actgatattt tcgaaattct atctcaaaaa ttgaatcact aagggggaga
     7321 tgtaaatggg tcaaacatta tttgacaagg tgtggaacag acatgtgtta tacggaaaat
     7381 taggcgaacc gcaactatta tacattgatt tacaccttat acatgaagtt acttctcctc
     7441 aagcatttga aggacttagg cttcaaaaca gaaaattaag acgcccagat ttaacatttg
     7501 caacactcga tcacaatgtt cctactattg atattttcaa tattaaagat gaaattgcaa
     7561 acaaacaaat cacaacatta caaaaaaacg ccatagattt tggggtgcat atttttgata
     7621 tgggttctga tgaacaaggt attgttcaca tggtgggacc tgagacagga cttacacagc
     7681 ctggcaaaac aatcgtttgt ggtgactctc acacagcaac acatggagca tttggtgcta
     7741 ttgcatttgg aattggaaca agtgaagttg aacatgtttt cgcaactcaa acgctatggc
     7801 aaacaaaacc caaaaactta aaaatcgata ttaatggcac cttaccaaca ggcgtctatg
     7861 ctaaggacat tattctgcat ttaattaaaa cgtatggtgt tgattttggt acaggctatg
     7921 ctttggaatt tactggtgaa acaattaaaa acctttcaat ggacggtcga atgactattt
     7981 gtaacatggc tatcgaaggt ggtgccaaat acggcataat ccaacctgat gatataacat
     8041 ttgaatatgt taaaggtcga ccatttgccg ataacttcgc taaatcagtt gataagtggc
     8101 gtgagctata ttctaatgac gacgcgatat ttgatcgtgt aattgaactt gatgtttcaa
     8161 cattagaacc acaagtgaca tggggaacta atcctgaaat gggtgttaat ttcagtgaac
     8221 cattccctga aatcaatgat atcaacgatc aacgtgcgta tgattatatg gggttagagc
     8281 caggacaaaa agctgaagat atcgacttag ggtatgtttt cctcggttca tgtacaaatg
     8341 ctagactatc agatttgatt gaagctagtc atattgttaa aggaaataaa gttcacccaa
     8401 atattacagc tatcgtcgta ccaggttctc gtacagtaaa aaaagaagct gaaaaattag
     8461 gtctagatac tatctttaaa aatgcaggat ttgaatggcg tgagccagga tgttcaatgt
     8521 gtttaggcat gaatcctgac caagtacctg agggcgtaca ttgtgcatct acaagtaatc
     8581 gaaactttga aggacgacaa ggcaaaggtg caagaacaca tttagtatcc cctgctatgg
     8641 cagcagcagc agctattcat ggtaaatttg tggacgtaag aaaggtggtt gtttaaatgg
     8701 cagcaatcaa acctattaca acatataaag gtaaaatagt ccctcttttc aacgacaata
     8761 tcgatacaga ccaaatcatt cctaaagtac acttaaagcg tatttcaaaa agtggctttg
     8821 gtccatttgc ttttgatgaa tggcggtact tacctgatgg ttcagataat cctgatttca
     8881 atccaaataa gcctcaatat aaaggtgcat ccatattaat tactggagat aattttggat
     8941 gtggttcaag tcgtgaacat gcagcttggg cacttaagga ctatggcttt catattataa
     9001 ttgcaggaag tttcagtgac attttttata tgaattgtac aaaaaatgca atgttaccaa
     9061 ttgttttaga aaaaaatgca cgtgaacatc ttgctaaata tgttgaaatt gaggtcgatt
     9121 taccaaatca aactgtgtca tcaccagaca agagtttcca ttttgaaatt gatgaaactt
     9181 ggaagaataa acttgtaaat ggcttagata acattgcaat caccctacac tatgaatcat
     9241 taatagaaaa atatgaaaaa tcactttaag ggagttgaat attatgacag tcaaaacaac
     9301 agtttctacg aaagatatcg atgaagcatt tttaagactt aaagatattg tcaaagaaac
     9361 acctttacaa ttagaccatt acttatctca aaagtatgat tgtaaagttt atttaaaacg
     9421 agaagattta caatgggtac gttcttttaa attaagaggt gcttacaatg ctatttctgt
     9481 tttatcagat gaagctaaaa gtaaaggtat tacatgtgcg agtgcaggta atcatgctca
     9541 aggtgttgcc tatacagcta aaaaacttaa tttaaacgct gttatcttta tgccagtcac
     9601 tacaccttta caaaaggtaa atcaagtaaa attctttgga aatagtaacg ttgaagttgt
     9661 actaactggt gatacatttg atcactgttt agctgaagct ttaacttata caagtgaaca
     9721 tcaaatgaac tttatagatc cattcaataa tgttcataca atttctggac aaggtacgct
     9781 tgctaaagaa atgctagaac aagcaaagtc tgacaatgtt aactttgatt atctatttgc
     9841 cgcaattggt ggtggtggtt taatttcagg tattagtacc tattttaaat cctattctcc
     9901 caacacaaaa attatcggag ttgaaccttc aggtgctagt agtatgtacg aatctgttgt
     9961 cgtaaataat caagttatca cattacctaa tatcgataaa tttgtcgatg gagcttctgt
    10021 agccagagtt ggcgatatta cttttgaaat tgccaaaaaa aatgtagata actacgtaca
    10081 agtagatgaa ggtgctgtat gttcaacaat tttggatatg tattcaaaac aagcaatagt
    10141 agcagaaccc gctggcgctt taagtgtaag tgcgcttgaa aactataaag atcatattaa
    10201 aggtaaaaca gtggtttgtg tcattagtgg tggtaataat gatattaatc gaatgaaaga
    10261 aattgaagaa cgttcattac tatatgaaga aatgaagcat tactttatct taaatttccc
    10321 tcaacgtcca ggtgcattga gagaatttgt aaatgacgta ttaggacctc aagacgatat
    10381 tactaaattt gaatacttaa aaaaatcttc tcaaaataca ggtactgtca ttattggtat
    10441 tcaacttaaa gattatgatg atttaataca actcaaacaa cgtgtaaatc atttcgatcc
    10501 ttccaatatt tatattaatg aaaataagat gttatattca ttgttaattt aacacatatt
    10561 aagaaaagca gtcataaatt gatttctaat tgaaatcatc ttatgactgc tttttattat
    10621 gctttacatt attgaggttg cacgctttgt ataccatcgt atatttcttt gagcctaaag
    10681 tcttcttcat tccccacccc attatttata ttttgaatgg cagttgaaat tactttgtca
    10741 taaatagcct tctcactttc tggtaaatta tgtcgtggta tactcatcaa agcatatgct
    10801 ttttgtttat cttcaatatt ttctatacga ttcttattat tattaaaatt ctcattagct
    10861 tcactaccat tattattaat attatgcgtg ttttcttttt catgtttgtt ttcttgcata
    10921 tgattcggga tttctgactt attttcagca tctgtaggaa aatatatttt ataagcaaaa
    10981 aatccacaca caatcaatga gcatacaaat aaaaaagtta acatcgtacc aataaatatt
    11041 ttcaaatccg ttctcctttc aaccattcat tcttacatgt atatttagta ttaaaaaatc
    11101 cttatagtct caacttgcct ggcaacgttc tactctagcg gaacgtaagt tggctaccat
    11161 cgtcgctaa
//
LOCUS       DAMZPP010000102        10941 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMZPP010000102 DAMZPP010000000
VERSION     DAMZPP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10941
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.102"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..2272)
                     /gene="clfA"
                     /locus_tag="POH73_001991"
     CDS             complement(<1..2272)
                     /gene="clfA"
                     /locus_tag="POH73_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HDI6887925.1"
                     /translation="MNMKKQEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSM
                     TQTENTSNESKSNDPSSVNAAPKTDNTNVSDSNTTTNTNSDETNVAQNPAQQETTQSA
                     STNATTEETPVTGEVTTTATNQANTPATTQSSNTNAEESVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDISTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGKDITNQLTNVTVGIDSGDTVYPHQAGYVKLNYGFSVPNEAVQGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLT
                     MPAYIDPENVTKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDAQNTSIKVYKVDNASDLSESYYV
                     NPDNFEDVTDSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRST
                     LYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASD
                     SDSASDSDSASDSDSTSDSDSTSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSVSDSDSDSDSDSESDSDSDSDSD
                     SDSDSDSESDSDSDSES"
     gene            complement(2536..3066)
                     /locus_tag="POH73_001992"
     CDS             complement(2536..3066)
                     /locus_tag="POH73_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6887926.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNNLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPNYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            complement(3205..3726)
                     /locus_tag="POH73_001993"
     CDS             complement(3205..3726)
                     /locus_tag="POH73_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887927.1"
                     /translation="MINIISAIGSIGTFIMALFYFVSVSVQLYQMKISFLPALGFNQI
                     LLEREGDQLNIMNSATEEHHHKDYIKLYNLGGGAAKKIAIEVLLGNDKVIQKKYVNIL
                     PSKEGYMLSINKNVYEELERTIENNGYEADLNVRMTYYHNVSHKQQEVILKGQIDRFN
                     TYNNKEIYDLQFI"
     gene            complement(3734..3889)
                     /locus_tag="POH73_001994"
     CDS             complement(3734..3889)
                     /locus_tag="POH73_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887928.1"
                     /translation="MIVKKLLKAIKQNFLYEQSNITFKMILFGYKRLSKMIFQFEINH
                     MNFLREG"
     gene            4325..5053
                     /locus_tag="POH73_001995"
     CDS             4325..5053
                     /locus_tag="POH73_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HDI6887929.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKNYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSGLDEV"
     gene            complement(5341..5955)
                     /locus_tag="POH73_001996"
     CDS             complement(5341..5955)
                     /locus_tag="POH73_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDI6887930.1"
                     /translation="MNDLSLSSFLKRSNKFMQFNCFICLILIIVFYIIGMNIQDFSDF
                     PSKDLNHKVTYNLNGFLEIFVNNAFIVPFVSLILSIIPIPYLYFIPTISTIYSLSVII
                     GVTFSYKLNEGIAIFIGILPHGILEIYLTSIELSMLFLLNAYIRKNSMNLFRKRKETL
                     PNFFVLLKSIVKCYLLIFLPVAFLCALIEITVTPTVYKFLTNII"
     gene            complement(6469..6830)
                     /gene="ssrA"
                     /locus_tag="POH73_001997"
     tmRNA           complement(6469..6830)
                     /gene="ssrA"
                     /locus_tag="POH73_001997"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(6921..7385)
                     /gene="smpB"
                     /locus_tag="POH73_001998"
     CDS             complement(6921..7385)
                     /gene="smpB"
                     /locus_tag="POH73_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDI6887931.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            complement(7407..9779)
                     /gene="rnr"
                     /locus_tag="POH73_001999"
     CDS             complement(7407..9779)
                     /gene="rnr"
                     /locus_tag="POH73_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDI6887932.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERSKTDRYQKKHSSKGQSKLIKGTLSQNKKGFAFLRPKDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLEEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGHTQLTVSIADVSYYVTEDSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPHVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     KEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIESERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGHKKKGKQRKGKNQRKNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            complement(9813..10553)
                     /locus_tag="POH73_002000"
     CDS             complement(9813..10553)
                     /locus_tag="POH73_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDI6887933.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPEEILQSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDKEMHHFKPTET
                     LKELSEALDTIKDQVDEVLDPILVIQAENDNMIDPQSANYIYEHVDSDEKDIKWYSQS
                     GHVITIDKEKEQVFDDIYQFLESLDWSE"
     gene            complement(10682..10915)
                     /gene="secG"
                     /locus_tag="POH73_002001"
     CDS             complement(10682..10915)
                     /gene="secG"
                     /locus_tag="POH73_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDI6887934.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
BASE COUNT         3552 a         2031 c         1527 g         3831 t
ORIGIN      
        1 ctgattctga gtcactgtct gagtctgatt cactatccga gtctgagtcg ctgtctgaat
       61 ctgagtcgct gtctgagtct gattcactat ccgagtctga gtcactgtct gaatctgaaa
      121 cactgtctga gtcgctgtct gaatctgaat cactatctga gtcggaatcg ctgtctgaat
      181 ctgagtcgct ttctgagtct gagtcgctat ctgaatctga gtcactgtct gagtcggaat
      241 cgctatctga atccgagtca ctgtcggaat ctgaatcgct atctgagtct gagtcactat
      301 ctgaatctga atcgcttgct gaatcggaat cactcgttga atctgaatca ctcgttgaat
      361 ctgaatcact cgctgaatct gaatcacttg ctgaatctga atcactcgct gaatctgaat
      421 cacttgctga atctgaatca ctcgctgaat ctgaatcact tgctgaatct gaatcgctcg
      481 ctgaatctga accactatct gatgtagaat cactacccga atctgaaccg ctatctgaat
      541 tagaatcgct gccagaatct gaaccactat ctgaacctgg gtcagaatct gaatcctctg
      601 gaattggttc aatttcaccc ggctcatcag gttgttcagg aacaacaggt ttatcgatac
      661 cgtcaccaga acctgatccg ttattaaatg ctacttcatt atcccatgac attgatcgcc
      721 aaattatatt cgaattatat ccatataaag ttgaacgtaa agctaaatca cctttactat
      781 taggatcaat atgcccatta acaactacaa tatatggtgt tgttatttga tcatcaggcg
      841 tattgaactc tactttatat tgatttggat ttgggaatgt aatattcaca ctatcagtga
      901 catcttcaaa gttatctgga ttcacataat aactttcaga caagtctgat gcattatcaa
      961 ctttatatac tttaatacta gtattttgtg catctattaa tgcattacta tccgtatttg
     1021 gttttaaatt acctgttaaa accggcgcaa taacgttatc tccacttgga ttgacataaa
     1081 ttgtctgacg atacgtatta tttgttttat cgatttggtc aattgtacct ttaatagata
     1141 agttataaaa cttaccatat ttttcataat ctactaatac tgttttgttt gctgttgtac
     1201 tacctatgcc agtagccaat gtcacattac ctgtctttgt aacattttca gggtcaatat
     1261 aagcgggcat ggtcaaagtt gctttaacat catctttagt atttacatag tctgtaaatg
     1321 tataaataac attaccatca ctatcgatta caccatttgc caatacttga tctccggcca
     1381 taattggtgg cactttagca gttgaagtta caccatttaa gtttaattct ttgggcacag
     1441 ttattttgaa tgtgtcacct tgaacagcct catttggtac tgagaaccca taattcagtt
     1501 tgacatagcc tgcttggtgc ggataaactg tatctccaga gtcaatacca actgtcacat
     1561 tcgtcaactg attcgtaata tctttgccag cagccggtgc atctgcagct acagccgcta
     1621 aactaaatgc tctcattcta ggcgcacttg tattaaccgc ttgattaact acatctttat
     1681 tacttgcatc tgtactctgt ggagctgatt cattgtttga aggtgttgct tcagttgaaa
     1741 tgtcttgcgt tgttgaaaca ttttccgcat ttgtagaatt ttgaggtgaa tttacagatg
     1801 atactgtatt agtatcatta gaagtcgttt cattacttgt ttgattcact gattcttccg
     1861 catttgtatt gcttgattga gttgttgccg gtgtattagc ttgattcgtt gccgtagtag
     1921 taacttcacc agttaccggt gtttcttctg tagttgcatt tgttgatgct gattgtgtcg
     1981 tttcctgttg tgctggattt tgcgctacat tcgtttcgtc actattagtg tttgtcgttg
     2041 tattagaatc actcacgttt gtgttgtctg ttttaggtgc agcattaacg ctacttggat
     2101 cattactttt gctctcatta ctcgtatttt ccgtttgtgt catactattt tcacttgcat
     2161 ctgcttcttt actgctgagt agtccaaaac cgattaacgt acctacaagc actgaagcca
     2221 cgccaatcga ttttttacga attgcgtgtt tttcttgttt cttcatattc attttattcc
     2281 ctctttttaa aaagtcattt tatattaact gtataccctt ttaatatata tttaatctct
     2341 attaatgcaa ttatacacca aaattgcata atggcaatta atttgtttta gaattttatt
     2401 aactacaata aatatttaca acgaaaagtt tttcattttc tctgttttaa aaattgcaaa
     2461 cgacaatgcg cgatttcatt gtaaacaatt tgtattatta atatgaactc tgcctaatgt
     2521 aatcctagct tgaaatcata ttttttcaaa agcagatgtg taatttatgg tacctgtttt
     2581 tcccgctaaa ctgttcactt ttaattcttt aattaaaaac gcttcatctg gtataccatc
     2641 atacggtgga taaatgtcgt acatacttgc gcgttgatat cctagattgg cataataatt
     2701 cggccatcct aatacactga taaaattata tccttttaat attgcttctc gttctaatgc
     2761 ttgaatcaat cgcttcccaa tacctttatt ttgatgatga atatcaacag atacaggtgc
     2821 taacactaat ccaatttccc gttgtgcctc gttatcaaga taaacttcac ttagtaaacc
     2881 gtgccctaca acttcatttt gaagaacagc tactaattct aagttattgt catacgtatc
     2941 acttagacga atttggtcta ctagttctga ttcattacca taaccatgtt cactattttc
     3001 aaatgccgtt ctaattaact ggtcaacttg agcaaagtca tgttgatgta tttgtctaat
     3061 ttgcatcttt gcccctcctt ttagttctat cttatagaaa aatgcattac aacataaagt
     3121 aatattttat agtaatgacg ccattatttc attaaaattg aatgatattt tttgcaataa
     3181 acaactatcc tcttaaatca attttcagat aaactgcaaa tcataaattt ctttattatt
     3241 ataagtatta aaacggtcga tttgaccttt taatataact tcctgttgtt tgtgacttac
     3301 attatgataa taagtcatac gtacattcaa atcagcttca taaccattgt tctcaatcgt
     3361 tctttctaat tcttcgtaca catttttatt aattgataac atgtaccctt ctttactagg
     3421 taaaatattc acgtactttt tctgaatgac tttatcatta cccaataaaa cctctattgc
     3481 aattttttta gcagcaccgc cacctaaatt atatagttta atataatctt tatgatgatg
     3541 ctcttctgta gcggaattca ttatattaag ttgatccccc tccctttcta ataaaatttg
     3601 gttaaaacct aaagctggca gaaagctaat tttcatttga taaagttgaa ctgaaactga
     3661 tacaaaataa aataaagcca taataaatgt tccaatagat cctatagctg aaataatatt
     3721 aatcattaag atatcaccct tctcttaaga aattcatatg attaatttca aactgaaaaa
     3781 tcattttact taatcgttta tatccaaaaa gaatcatttt aaacgttata ttcgactgct
     3841 catataaaaa attttgcttt atagccttaa gtaatttttt aactatcaag tacgcttatt
     3901 attacggcaa tctatttgca ttgactaaaa aatacaataa cgcttttgag ggcacccatc
     3961 tttgaccagt atttcaaaat taataaaaca tcttcttcaa tacatattca tctcatacaa
     4021 ataagttgaa tttttttatt ttttataaac gtaatttctt aacaaatcaa aattttacat
     4081 ttaaatcttt cgatatatta caagtttttt aaaaaagtaa tatatttcta tcaataaaag
     4141 taatataata ttagtaatta tagttccatt aatttattgc aacaatacat agaatcatta
     4201 ttacaagaat tatttaatat catcttagaa tctcacaaaa tatcaacttt gtttaattac
     4261 tcaaaacgat aagtcgttta gagtttttaa aataaatttt tgcgaaataa aggagacatg
     4321 ttaaatgaaa aaagtaatgg ggatattatt agcaagtaca cttatcttag gtgcttgtgg
     4381 acatcatcaa gatagtgcaa aaaaagagag cactagtcac aaaaagaaag aaaatgacaa
     4441 tgaagaatta aatgaagaac ttaaagaatt taaaagcaaa aaaaatatgg atataaaaat
     4501 taaaggcgat actattgtta gtgacaaatt tgaagctaaa ataaaagaac cgtttatcat
     4561 caatgaaaaa gatgagaaaa agaaatatat cgcttttaaa atggaaatta ctgctaaaaa
     4621 agacgataaa gatttaaatc catcttctat ttctcatgac tatattaata tcactcaaga
     4681 tgataaaaat acagtaaata aattaagaga tggttatctt ttaagtgata aaaattataa
     4741 agattggaca gaacataacc aagatcaaat taaaaaaggc aaaactgcac aagccatgtt
     4801 tatctatgag ttaagaggtg atggaaacat taatttaaat gtccataaat actcagaaga
     4861 taaaacagtt gattctaaat cattcaaatt tagtaaactt aaaaccgaag atttttctca
     4921 tagagcggaa acaagggaag aagtagaaaa gaaagaaaaa gaatttgaag aagagtacaa
     4981 aaaagaacaa gaacgagaga aagaaaaaga aaagcaaaaa gatgacgacc acagtggttt
     5041 agatgaagta taaatttatg aatgctcgca ttcagcgtta taatctttgt tgttattatt
     5101 gtcattataa attagcttac ttacactcat aatgacacac taatttaatt gttaaatata
     5161 acaaaaaaga gtagctcgcc tactcttttt tgttgttttg aaatggtcat ttttgaaatg
     5221 actcgattat agtttcacga ataccatgca acatttttcg tgaactcata ttttacaaat
     5281 caatatttat cgatcaaaaa taaacctata cacatagtta gctatgccct tttaaaagtt
     5341 ctatattata ttagttaaaa atttgtaaac agttggtgta acagtaattt caattaaagc
     5401 gcataaaaat gccacaggta aaaatattag caggtaacac tttactattg attttaataa
     5461 aacaaaaaaa tttggcaatg tctcttttct ttttctaaat aaattcattg aattttttct
     5521 gatgtacgca tttaataaga ataacattga tagttcaatg cttgttaagt atatttctaa
     5581 aataccatga ggcaaaatac caataaaaat agctatccct tcgtttaatt tatatgaaaa
     5641 tgtaacccca ataataaccg acaatgaata aattgtagat attgtaggaa taaaatacaa
     5701 atatggtatt ggtattatcg ataatataag tgacacaaaa ggaactataa aagcattatt
     5761 aacaaatatc tccaaaaacc cattaagatt ataagtcact ttatgattta aatctttact
     5821 aggaaaatca ctaaagtcct gtatattcat accaattatg taaaatacta ttattagaat
     5881 caaacaaata aaacaattga attgcataaa tttgttactg cgtttcaaaa atgaagataa
     5941 actcaagtca ttcattgatt atgcccccat ctataaatac tatttcacta aatagatacg
     6001 ttcactcgca aacgatatca tccttaaaaa tctattaaaa accagctata ttaaaacctt
     6061 taatttacca gctaaaacta tcacaacttt tcctcattac atttaaacaa tatatcaaat
     6121 ttaagtcaat taaaagaaaa atataatatt tataaaaatc ttgattctaa ttctaaacta
     6181 tatatttaat tctcttattt atgatacttc tttagcttaa ttaattttgc aaataaaatt
     6241 tacattttaa aactcaaagc tcaacttttt tgacacttat ttgacacata ttctttcaac
     6301 ccttaacgca aaatcttata tgtacacaag tattaatttt tcattcatga aaatttgaac
     6361 ctaatcagat actatcatat caacgttcac agctaattta accttcctca caagaagaga
     6421 taaaaaacaa ataaaaaagc ccacatccgc aaaggttgta ggctacaaat atggagacgg
     6481 cgggatttga acccgcgtcc agaggtcctg atacacatct ttctacgtgt gtagtttatc
     6541 gaaaagtttc gcatcatgaa aagtgataaa caaccaacat gatgctagct tgattaagtt
     6601 tcttctaaac agacttcaaa cggcagtgtt tagcatatcc tattaaggtt gaatcgcgtt
     6661 aacagcacat aggaaatgct gttaggcgat gcagagtgcg attaggcagc tactgcgaaa
     6721 ttattgtttg atttgccagt tattataaac tgtgtgtgtt gatgacgaag acaaccctcc
     6781 gacacgctta atgagctcgg gggacccctg tcgaatccag aaacgtcccc gaaataataa
     6841 atcaaacaaa tcatactttt gcaatttaca aaagcacata taactatatc aaatatagcc
     6901 cgattaagca actaaatcgc ttaataacgg gctttcatat cgcgcgcaac atctcgtttg
     6961 actgcttttt ctttcaaagc ttgacgttta tcatattttt tcttacctcg tgcaacacca
     7021 agtaatactt tacaatgtcc atgcttcaaa taaagcttta acggcacaat cgaataacca
     7081 atctcacgtg tttgatcacc caatttaatg atttcacgct tatgcaataa taattttcga
     7141 gaacgaagag gatcgtgatt aaaacgattc ccttcttcgt atggtgctat atgcatatta
     7201 ttcaaataca tttcaccgtt tttaacttgc gcataactat ctttaaggtt agcactacct
     7261 cggcgaattg attttatttc tgtgccttgc aatacaattc ccgcttcaat cgtgtcttca
     7321 atattataat catgtctagc tttacgattt tccgctaatg tacctggtga tttcttctta
     7381 gccattcata ctcacctcat tgctgcttat tttttcttac gacgtgcttt ctttttcaca
     7441 cttttatctt tataaaatgg cttatgttta ctgtttcctg atttgtcatt tttacgttgg
     7501 tttttacctt tacgttgctt accttttttc ttatgacctt tatcatcaga tttcgattta
     7561 tctaatgatt taccacgcgt tttagcttga attgtcttac ctcgtgctgg gcgctgtgat
     7621 cgatcatttt tcggtaaagg catacctaca atttgaaaat caattaatcg ttcatctaca
     7681 tcaacatgcg tcactttaac cttaactgtg tcaccaattc taaatacttt agcttgacgc
     7741 tcaccaatta atgccatttg acgctcttca aaacggtaat aatcatcagt catattcgca
     7801 atatgaacca taccttctat cgtatttggc aattcaatga acataccgaa gttagctact
     7861 gagctgacaa taccttcaaa ttcatcacca atatgttgaa tcatatattc tgcttttttc
     7921 aattcatcag tatcacgttc tgactcaata gcacgacgtt cacgtttaga agtatgttca
     7981 gctaactcag gcaatttgtc ttcccaacgc ttcacttctt tgttatccat tgatttctca
     8041 attaaatact tacggattaa acgatgaact gttaaatcag gataacgtct tattggtgat
     8101 gtaaaatgcg tataatattc agctgataag ccaaaatgtc ccaagttcac atcatcataa
     8161 tgcgcttgtt gcattgaacg taacatcatt gttgaaatga ccatttgttc aggtcgacct
     8221 tctacttctt cttggacctt ttgaagtgtt gttggatgaa tatcttcgcc tgttccttta
     8281 atcataatgc caaagttagt aataaaatca aagaattgtc ttaagcgatc tgatttaggt
     8341 tgctcatgca ctcggtaaat aaatggtaca tctaacttac taaaatgttc agcaactgtt
     8401 tcatttgcaa ttaacataaa tgactcaata agacgttcgc cttcaccacg ttgtctcaat
     8461 tgaacatctg taggtatacc atcttcgtta actagtactt tcgcttcgct aatatcaaaa
     8521 tcaatttcac cacgacgttt tctcatttga atcaaacgat tagataaatc ttgtgctaaa
     8581 tctaacatag gcgtaatttc tttatattgc tcgcgaatgt taggatcctt ttcagtaata
     8641 atctggttaa ctgcatcata cgtcattcga taatcagaat gtataacact atcaaaaatt
     8701 tcatgtttaa caacacgacc acttgcatca atttccatgc gacagcttaa cgttaaacga
     8761 tcaacgtgag gatttaatga acaaatacca ttgcttaatc gatgtggaat catcggaatc
     8821 acacggtcaa caagatatac acttgtcgct ctatcataag cttctttatc taaagctgaa
     8881 tcttccgtta cataataact gacatcagca atacttacag ttaattgcgt atgaccgttc
     8941 gctaactttt taacactgat tgcgtcatct aagtctttag catccgcacc atcaattgtg
     9001 attgtcaatt catcacgtaa atcatgacgg cctttaattt cagtattttc aatatgatca
     9061 ggtactgctt cagcttcttc taatacttca tcaggaaact caatttcaat accatgttga
     9121 taaataatag ataaaatatc tacacctgga tcatttttat gtcctaatat tgctgaaata
     9181 tgcccttcag gattatctga accatcagca tacttagtaa tttgaactag taccttatgc
     9241 ccatctactg cacctaaact ttgaccttta ggaatgaaaa tatcttgcat aatacgttta
     9301 tcatctggaa taacaaagcc gaaatgtcta gcttcactat acgtaccaac aacttgagtt
     9361 acagaatgct tctcaatcga cttaacttcc ccttcaattt tacctttatg ttcaccttta
     9421 gattgatgta tttctacaat tacagtatct ccgtctaagg cacgattaat ttttgttggg
     9481 ggaataaata tatcttccat atcctcatct ttaggtctta ggaaagcaaa acctttcttg
     9541 ttttgactta acgttccttt tatcaatttc gattgacctt tggaactatg ctttttttgg
     9601 tatctatctg ttttcgaacg ttcgattaat cctgattgtt ctaactccac aagcacctta
     9661 attaaatctc taaacgagtc ggcactgctt aaacctaatg catcttgaaa atctgaaact
     9721 gacataggtt catattcagg ttgattaata atctcttcta ttgattgctt taaattcatt
     9781 atgcccctcc tttctaatgt taaaatctct ttttattctg accagtctaa tgactctaaa
     9841 aattgataaa tatcatcgaa aacttgttct ttttctttat caattgtaat aacatgtcct
     9901 gactgactat accatttgat atctttttcg tcagagtcta catgttcata tatataattt
     9961 gcggattgtg gatcaatcat attgtcgttt tctgcttgaa tcactaaaat tgggtcaagc
    10021 acctcatcaa cttgatcttt aatcgtatct aatgcttcac ttaattcttt taaagtttca
    10081 gttggtttga aatgatgcat ttctttgtca atcgtttctt gatctttacc ctcatacttt
    10141 ttaaaattgc gtgcatattc taaaaagcct tcataaatgg caccttcagt tttgccaccc
    10201 ataggagcac acatcgttac aatacccttt acatctcgat ttaagcttaa ttttaatgca
    10261 aaatcgccac ctaatgacaa gccagcaaca acgatttcat cataaccttg ttcaacaagg
    10321 tagtcatagc catctaaggc atctttaaac caaacaaaag gacttgattg aagtatctct
    10381 tctggtggtg ctgcatgacc ttcatattgt ggtgcatacg atgtataacc tttcttttgt
    10441 aaaaatcgac ctaattgacg aacatcagac gaattgcctg taaaaccatg tagtaataac
    10501 acggcacgtt taccttcctc aaaaaagaaa ggctttggta actttatttg cataaatcta
    10561 acgctccttt ttcacaatta acaaacgtac ttaattaaac aatatgtttc taataattga
    10621 acatgagtcc tttattattc taattataaa taaaaaagcc cgacatttac atcgccggac
    10681 cttacatacc aagataactt atgcaaatca taagtacaaa aaataatatt gataaaataa
    10741 ttgttaatct atttaagaat aaatcgacgc cacgttgttt ttgtttaccg aataactgct
    10801 cagcaccacc actgatggca cctgaaagtc cactgctttt accttcttgg agtagtacaa
    10861 cagttattaa tgcaatacaa tcaatgatta ataatacgat taaaaatgta tgcataaatt
    10921 gtcctccgtt ccttatatac g
//
LOCUS       DAMZPP010000103        10896 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMZPP010000103 DAMZPP010000000
VERSION     DAMZPP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10896
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.103"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            26..262
                     /locus_tag="POH73_002002"
     CDS             26..262
                     /locus_tag="POH73_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HDI6887935.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            531..2516
                     /gene="uvrB"
                     /locus_tag="POH73_002003"
     CDS             531..2516
                     /gene="uvrB"
                     /locus_tag="POH73_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDI6887936.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            2524..5370
                     /gene="uvrA"
                     /locus_tag="POH73_002004"
     CDS             2524..5370
                     /gene="uvrA"
                     /locus_tag="POH73_002004"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDI6887937.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNINLSE
                     QDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            5981..6913
                     /locus_tag="POH73_002005"
     CDS             5981..6913
                     /locus_tag="POH73_002005"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDI6887938.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            6919..7761
                     /locus_tag="POH73_002006"
     CDS             6919..7761
                     /locus_tag="POH73_002006"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDI6887939.1"
                     /translation="MGGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALV
                     KAGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPGEIIKIWHGGIAIHGGLI
                     GGFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGPVSRAFLEQL
                     HLPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIG
                     RFFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPT
                     RKVK"
     gene            7769..8254
                     /locus_tag="POH73_002007"
     CDS             7769..8254
                     /locus_tag="POH73_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI6887940.1"
                     /translation="MRKFLSKEYHRTNPLWHVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQILNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            8262..9701
                     /locus_tag="POH73_002008"
     CDS             8262..9701
                     /locus_tag="POH73_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDI6887941.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMANQKFKQREYKRAAEYFEKVLEL
                     SPDDLEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLKEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYHNELFVSENDLMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            9768..10703
                     /gene="trxB"
                     /locus_tag="POH73_002009"
     CDS             9768..10703
                     /gene="trxB"
                     /locus_tag="POH73_002009"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDI6887942.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSAPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
BASE COUNT         3894 a         1477 c         2183 g         3342 t
ORIGIN      
        1 taaataaaag aaaagaggaa aatgaatgat atggtatttt agcgcagcat tctttccatg
       61 tgtcttggta gtattgttta gtgtaataac aagaagtaaa tgggtcggta ctattctgac
      121 attaatttta attggtgcct caatctataa agagtatttc cataacgagt ggattatttt
      181 tattgatgtg gtgtcattat tagctggtta tttaattata gatcaactcg aatttcataa
      241 acatcaagat gaagatcgct aagattaact ttaaaataat gtttcaaaca aatttgttga
      301 aacaaaatga tgattaatat aatgtgtatt tacatactaa aaataacaag ataaacgatt
      361 tgatttaagg caagcatagt tagcacaact atgtttgttt ttctttgttc gacattttta
      421 cgaacaaacg tttgcttttt gtgtgactac tttgctaaaa tatgtaatga gaaagcgaag
      481 agttgagcgg aaatataaat aatacgttga aagaggaggc atatgtgact atggttgaac
      541 attatccttt taaaatacat tctgattttg agcctcaagg tgaccaaccg caagcaatta
      601 aggaaatcgt ggaaggtatt aaagcgggga aaagacatca aactttatta ggtgcaactg
      661 gcacagggaa aacatttacg atgagtaatg ttattaaaga agttgggaaa ccaacgttaa
      721 ttatcgcgca taacaaaaca ttagcaggac aattatatag tgagtttaaa gaattttttc
      781 ctgaaaacag ggtggaatac tttgtaagtt actatgatta ttatcaacct gaggcatacg
      841 taccgtctac tgacactttt attgaaaaag atgcctcaat caatgatgaa attgatcaac
      901 tacgacattc tgctacaagt gcattatttg aacgcgatga tgtaattatt attgctagtg
      961 taagttgtat atatggttta ggtaatcctg aagaatataa agatttagta gtaagtgttc
     1021 gagttggtat ggaaatggat agaagtgaat tacttagaaa acttgtagat gtgcaatata
     1081 cacgaaatga catcgatttc caacgaggaa cgtttcgagt gcgtggtgat gttgttgaaa
     1141 tattcccagc ctctaaagaa gaactttgta taagggttga gtttttcggc gatgagattg
     1201 accgtattcg agaagttaac tacctaactg gtgaagtgtt gaaagaaaga gaacattttg
     1261 cgatattccc agcttctcac ttcgtaacac gtgaagaaaa gttgaaagtt gcgattgaac
     1321 gtattgaaaa agaattggaa gaacgattga aagaattacg agatgagaat aaattactag
     1381 aagcgcaaag gttagaacag cgtaccaact atgatttaga aatgatgcga gagatgggat
     1441 tctgttcagg aattgaaaac tattccgtac atttaacttt gcgaccactg ggttcgacac
     1501 catatacttt attggattac tttggcgatg attggttagt aatgattgat gaatcacatg
     1561 tgacattacc gcaagttcga ggcatgtata acggagacag agcgcgtaaa caagttttgg
     1621 tggatcatgg atttagatta ccgagtgcat tagataaccg tcctcttaaa tttgaagaat
     1681 ttgaagaaaa gacaaaacaa cttgtgtatg tatctgcaac gcctggacca tacgaaattg
     1741 aacatacgga taagatggtt gaacaaatta ttcgtcctac tggtttactg gatcctaaga
     1801 ttgaggttag acctactgaa aatcaaattg acgatttatt aagtgaaatt caaacaaggg
     1861 ttgagcgtaa tgaacgcgta cttgttacaa cgctcactaa aaagatgagt gaagatttaa
     1921 ccacatacat gaaagaagcg ggtattaaag ttaattatct gcattcagaa atcaagacat
     1981 tagaacgaat cgaaataatt agagacttac gaatgggtac atatgatgtt atcgtaggta
     2041 ttaatttatt aagagagggt attgatatac cagaagtttc tctagttgtc atattagatg
     2101 cagataaaga aggattttta cgttctaacc gctcattaat tcaaacaata ggtagagctg
     2161 cgcgtaacga taaaggtgaa gtcattatgt atgccgataa aatgactgat tcgatgaagt
     2221 atgcaattga tgagacacaa cgtcgtcgag aaatacagat gaaacataat gaaaaacatg
     2281 gtattacacc taaaacaatt aataaaaaaa tacatgattt aattagtgct actgttgaaa
     2341 atgacgaaaa taatgacaaa gcacaaactg tgatacctaa gaagatgacg aaaaaagaac
     2401 gtcaaaagac aatcgacaat atagaaaaag aaatgaaaca agcagcgaaa gatttagatt
     2461 tcgagaaagc tacagaatta agagatatgt tatttgaatt aaaagcagaa gggtgacaag
     2521 taaatgaaag aaccatccat agtagtaaaa ggtgctcgtg cgcataactt gaaagatatt
     2581 gatatcgaac tacctaaaaa taaattaatt gttatgacag gattatctgg gtcaggtaaa
     2641 tcgtcattag cattcgatac tatatatgct gaaggacaac gacgttatgt tgaatcatta
     2701 agtgcctatg cgcgtcaatt tttagggcaa atggacaaac cagatgttga tactattgaa
     2761 ggattatcgc cagcaatttc aatagatcaa aaaacaacaa gtaaaaatcc aaggtcaact
     2821 gttgcaacag taacagaaat atatgattat atacgtttgt tatatgcacg tgttggtaaa
     2881 ccttactgtc caaatcacaa tatagaaatt gaatcgcaaa cagtacaaca aatggttgac
     2941 cgcattatgg aattagaggc acgtacaaag attcaattat tagcacctgt catcgctcat
     3001 cgtaaaggta gtcatgaaaa gctaatcgaa gatattggta aaaaaggtta tgtacgttta
     3061 agaatcgatg gcgaaattgt tgatgtaaat gatgtaccta cattagataa gaacaagaat
     3121 catacaatag aagttgttgt agaccgatta gttgttaaag atggaattga aacacgacta
     3181 gctgactcta tagaaactgc gttagagctt tcagaaggac aattaacagt cgatgtcatt
     3241 gacggggaag accttaagtt ttcagaaagc cacgcttgtc ctatatgtgg attttcaatc
     3301 ggagaattag aaccaagaat gtttagcttt aacagtcctt ttggtgcttg tccaacatgt
     3361 gatggcttag gtcaaaagtt aacagtcgat gtagacttgg ttgttcccga caaagataag
     3421 acgctaaacg aaggtgcaat agaaccatgg ataccgacga gttctgattt ttatccaaca
     3481 ttgttaaaac gtgtttgtga agtttataaa atcaatatgg ataaaccttt taaaaagtta
     3541 acagaacgtc aacgtgatat tttattgtat ggttcgggtg acaaagaaat tgaatttaca
     3601 tttacacaac gtcagggtgg tactagaaaa cgaacaatgg ttttcgaggg tgtagttcct
     3661 aatataagta gacgattcca tgaatctcct tcagaatata cacgtgaaat gatgagtaaa
     3721 tatatgactg aactaccttg cgaaacttgt catggaaagc gattgagtcg tgaagcgtta
     3781 tctgtttatg taggtggttt aaatattggt gaagtagtcg aatactcaat tagtcaagcg
     3841 ctgaactatt ataaaaacat taatttgtca gaacaagatc aagcgattgc aaatcaaata
     3901 ttgaaagaaa ttatttcccg actcactttt ttaaataatg tgggacttga atatttaacg
     3961 ctaaacagag cttcaggtac actttcaggt ggtgaagcac aacgtattcg attggcaacg
     4021 caaattgggt cgcgtttgac tggtgtctta tatgtattag atgagccatc aattggactg
     4081 catcaaagag ataatgatcg attaattaat acacttaaag aaatgagaga tttaggaaat
     4141 actttaattg tagttgaaca cgatgatgat acaatgcgtg cggctgatta cttagtggac
     4201 atagggcctg gtgctggtga gcatggagga cagattgtgt ctagtggtac acctcaaaag
     4261 gtaatgaaag ataaaaaatc tttaacagga caatacttga gtggtaagaa acgtattgaa
     4321 gtacctgaat atcgcagacc ggcttcagat cgtaaaattt ctatacgtgg agctagaagt
     4381 aacaacctta aaggggttga tgtggacata ccactttcaa tcatgacggt cgttacaggt
     4441 gtatcaggtt ctggtaaaag ctcattagta aatgaagtat tatacaaatc attagctcaa
     4501 aaaatcaata aatctaaagt aaagccagga ttgtacgata aaattgaagg tattgatcaa
     4561 cttgataaaa ttattgatat tgatcaatca ccgataggta gaacgccacg ctctaatcca
     4621 gcaacatata ctggcgtgtt tgatgatata cgtgatgtgt ttgcgcaaac aaatgaagct
     4681 aaaattcgag gatatcaaaa aggtcgtttt agttttaatg taaaaggtgg acgctgtgaa
     4741 gcttgtaaag gtgacggtat tattaaaatt gaaatgcatt ttttacctga tgtttatgtt
     4801 ccttgtgaag tgtgtgatgg taaacgatat aatcgcgaga cactagaggt tacttacaaa
     4861 ggtaaaaata ttgctgacat tttagaaatg actgttgaag aagcaacaca attttttgaa
     4921 aatattccta agattaagcg caagctacaa acactagttg atgttggtct tggatacgtc
     4981 acattaggtc aacaagctac aacattatca ggtggtgagg ctcaacgtgt gaaacttgca
     5041 tctgaacttc ataaacgttc aactggtaaa tctatttata tcctagacga accgacaaca
     5101 gggttacatg ttgacgatat tagtagatta ttaaaagtat taaaccgatt agttgaaaat
     5161 ggtgacactg ttgtaattat tgaacataac ctagatgtta tcaaaacagc agactatatt
     5221 atagacttag gtcctgaagg tggtagtggc ggtggtacta ttgttgcgac tggaacaccc
     5281 gaagatattg ctcagacaaa gtcatcatat acaggaaagt atttaaaaga agtacttgaa
     5341 cgagataaac aaaatactga agataaataa gattaaaaga agtgaaggat gttgtaaatt
     5401 tatccttcac ttctttttat taatttagta atgaatagta gaaagaaaag atgcgtaaaa
     5461 agaattatgt taagataagg tcaatctaga gtagctaaac ataaatcgaa actgggagcg
     5521 ggacagaaat gataaagaat cactaatgat ttattatgta gtggttcttt gtcattagcc
     5581 acagctattg tgtacttaaa aataggaatg catgagtgca actcatgcat aagaaatact
     5641 aatttctaaa gaaaaagtat ctctttatgt tgggggcccg ccccaacttg cattgtctgt
     5701 cgaaattgag aatccaattt ctctgtgttg gggccccgcc aactaattcg aatttatcat
     5761 tttagagctt aggtcattga tttatggctc ggacttttat ggcgatatga aacatgtaaa
     5821 ttaagcaagc aataaattaa tgattgatat tgacttgtaa aataataaca ataatgaaca
     5881 attaatattt attttagctt ttcaatgtag attggtgtta tatttttgat atgataagaa
     5941 gagatgtaag agtagggata aatacaattg aggtgaaccc atgttaacga cagaaaaact
     6001 agttgaaaca ttaaagttag atttaatcgc tggtgaagaa gggctatcga agccaattaa
     6061 aaatgctgat atatcaagac cgggcttaga gatggcaggt tatttttcac attatgcgtc
     6121 agatagaata caactattag gaacaacgga actatcgttt tacaatttat taccagataa
     6181 ggatcgcgca ggtcgtatgc gtaaactatg cagaccagaa acgcctgcaa ttattgtgac
     6241 acgtggattg cagccaccag aagaattagt tgaagctgca aaagaattaa atacgccact
     6301 tatagttgct aaagatgcca ctacaagttt aatgagtcgc ttaacgacgt ttttagagca
     6361 tgcacttgca aagacgacat ctttacatgg agttttagta gatgtttacg gtgttggtgt
     6421 actaattacc ggtgattcag gaataggtaa aagtgagact gcgttagaat tagtgaagcg
     6481 tgggcataga ttagtagcag atgataatgt agaaatacgt caaattaata aagatgaact
     6541 aatagggaaa ccaccaaagt taatagaaca tctattagaa atacgtggac taggtattat
     6601 caatgttatg actttatttg gcgcgggttc aatattaact gaaaaacgaa ttagattaaa
     6661 tattaatttg gaaaactgga acaagcaaaa gttatatgac cgcgtaggtc ttaatgaaga
     6721 gacgttaagt attttagata ctgaaataac taaaaaaaca atacctgtaa gacctggtag
     6781 aaatgttgcg gtaattattg aggtcgctgc aatgaactat cgattaaata tcatgggcat
     6841 taacactgcc gaagaattta gtgaaagatt aaatgaagaa attatcaaga acagtcataa
     6901 gagtgaggag taggttgaat gggtggtatt gtatttaact atatagatcc tgtggcattt
     6961 aacttaggac cactaagtgt acgatggtat ggaattatca ttgctgtcgg aatactactt
     7021 ggttactttg ttgcacaacg tgcactagtt aaagcaggat tacataaaga tactttagta
     7081 gatattattt tttatagtgc actatttgga tttatcgcgg cacgaatcta ttttgtaatt
     7141 ttccaatggc catattacgc ggaaaatcca ggtgaaatta ttaaaatatg gcatggtggt
     7201 atagcaatac atggcggttt aataggtggc tttattgctg gtgttattgt atgtaaagtg
     7261 aaaaatttaa acccatttca aattggtgat atcgttgcgc caagtataat tttagcgcaa
     7321 ggaattggac gctggggtaa ctttatgaat cacgaggcac atggtggacc tgtgtcacgc
     7381 gcttttttag aacaattaca tttgcctaat tttataatag aaaatatgta tattaacggc
     7441 caatattatc atccaacatt cttatatgaa tccatttggg atgtcgctgg atttattatc
     7501 ttagttaata ttcgtaaaca tttaaaatta ggagaaacat tctttttata tttaacttgg
     7561 tattcaattg gtcgattctt tatagaagga ttacgtacag atagcttaat gctcacaagt
     7621 aatattagag ttgcacaatt agtatcaatt cttttaattt taataagtat aagtttaatt
     7681 gtatatagaa ggattaagta taatccaccg ttgtatagca aagttggggc gcttccatgg
     7741 ccaacaagaa aagtgaagta gtgatatttt gagaaaattt ttatcaaaag aatatcatcg
     7801 tacaaaccct ttatggcatg tataccgtct tgttaaattt tcgaaagttt ttaagaatgt
     7861 aatcatcatt gaattttcga aatttattcc aagtatggta ctgaaaagac atatatataa
     7921 acaaatttta aatattaata tcggtaatca atcgtcgata gcttataaag taatgttaga
     7981 tattttttat ccagaactga ttacgattgg tagtaacagt gttattggtt acaatgtaac
     8041 aattttgacg catgaagcat tagttgatga atttcgttat ggaccagtga cgataggatc
     8101 taacactttg attggtgcaa atgctaccat tttacccggt ataacgattg gtgacaatgt
     8161 aaaagttgca gctggtacgg ttgtttcaaa agatataccg gataatggat ttgcatatgg
     8221 caaccctatg tatataaaaa tgattaggag gtgacaattt tatggcgcaa aagaataata
     8281 atgtaattcc aatgactttt gatgatgcat tttatcgtaa aatggctaat cagaagttta
     8341 aacaaagaga atataaacga gctgctgaat actttgaaaa agtgttagaa ttgtcacctg
     8401 atgatctgga aattcaaatt gattatgcac aatgtctagt gcaacttggt attgctaaaa
     8461 aagcagaaca tttattttat gacaatatta tttataatag gcatctagaa gatagctttt
     8521 atgaattgag tcagctcaac attgaagtta acgaaccaaa caaggcattc ttgtttggta
     8581 ttaattatgt tattgttagc gacgaccaag attatagaga tgaattagat caaatgtttg
     8641 atgtgaaata tcaaagtgaa gaacaaattg aacttgaagc tcaattgttt gtagttcaaa
     8701 tactattcca atatcttttt tctcaaggtc gattaaaaga tgcaaagaat tatgtcttac
     8761 atcaaccaca agaagttcaa gatcatcgtg tcgtacgtaa tttactggca atgtgttatt
     8821 tatatctcgg cgaatatgat acggctaaag cattgtacga agcactatta aaagaggata
     8881 gtacagatat atatgcatta tgccattata ctttgctact ttataacact aaggaaaatg
     8941 aacaatacca aaagtattta aaaatattaa ataaagttgt acctatgaat gacgatgaaa
     9001 gttttaaatt aggtattgtt ttaagttatt taaagcagta tcgtgcttca caacaattgt
     9061 tgtacccttt atataaaaaa gggaaatttt tatcaattca aatgtacaat gctttagcat
     9121 ataattatta ttatttaggt gaagaagacg aaagtcatta ctactgggat aaattgaagc
     9181 aaatttctaa agtggaaatt ggacatgctc cttgggtaat tgaaaatagc aaagaagttt
     9241 ttgaccaaca tattttgcca ttacttcaaa gtgatgacag tcattatcgt ttatatggta
     9301 tttttttatt ggatcaatta aatggtaaag aaattgtgat gacggaaagt atttggcagg
     9361 ttctggaaaa tctaaataat tatgagaaat tgtatttaac gtatttagtt caaggtttaa
     9421 cgctcaataa attagacttc attcatcgcg gcttgttaac gctttaccat aatgaattat
     9481 ttgtaagtga aaatgattta atggttgcat ggattaatca aggtgaactc ataattgctg
     9541 aaaaagtaga tttaactgat gttgagccat atatcggtgc gtttatttat ttgtatttta
     9601 aaaatcaacc tcgaaacgtt acaaagaagc aaattacaac atggttaggc ataacacaat
     9661 ataaactgaa caaaatgatt gaatttctct tgagcatata gatttatgaa aagttagatt
     9721 tattatataa tgcgcataat gattaataat gaggaggcgt taataaaatg actgaaatag
     9781 attttgatat agcaattatc ggtgcaggtc cagctggtat gactgctgca gtatacgcat
     9841 cacgtgctaa tttaaaaaca gttatgattg aaagaggtat tccaggcggt caaatggcta
     9901 atacagaaga agtagagaac ttccctggtt tcgaaatgat tacaggtcca gatttatcta
     9961 caaaaatgtt tgaacacgct aaaaagtttg gtgcagttta tcaatatgga gatattaaat
    10021 ctgtagaaga taaaggcgaa tataaagtga ttaactttgg taacaaagaa ttaacagcta
    10081 aagcggtcat tattgctaca ggtgcagaat acaagaaaat tggtgttccg ggtgaacaag
    10141 aacttggtgg acgcggtgta agttattgtg cagtatgtga tggtgcattc tttaaaaata
    10201 aacgcctatt cgttatcggt ggtggtgact cagcagtaga agagggaaca ttcttaacta
    10261 aatttgctga caaagtaaca atcgttcacc gtcgtgatga gttacgtgca caacgtattt
    10321 tacaagatag agcattcaaa aatgataaaa tagactttat ttggagccat actttgaaat
    10381 caattaatga aaaagacggc aaagtgggtt ctgtgacatt aacgtctaca aaagatggtt
    10441 cagaagaaac acacgaggct gatggtgtat tcatctatat tggtatgaaa ccattaacag
    10501 caccatttaa agacttaggt attacaaatg atgttggtta tattgtgaca aaagatgata
    10561 tgacaacatc agcaccaggt atttttgcag caggagatgt tcgcgacaaa ggtttacgcc
    10621 aaattgtcac tgctactggc gatggtagta ttgcagcaca aagtgcagcg gaatatattg
    10681 aacatttaaa cgatcaagct taaatcgaag tcgaattaag atgttgcgtt gtaaattatt
    10741 tggatattta ttttaatagt gacatcacat tgttaaaata atgtcttact tttaaattaa
    10801 agcaaattat atagaaaact agaacttagt acgtatcatt tgtgcgtttc aatgagttct
    10861 agttttttta tatgttatat taaacttata acttta
//
LOCUS       DAMZPP010000104        10847 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMZPP010000104 DAMZPP010000000
VERSION     DAMZPP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10847
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.104"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..253
                     /locus_tag="POH73_002010"
     CDS             <1..253
                     /locus_tag="POH73_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDI6887943.1"
                     /translation="HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRL
                     FTSEVILQGEAIAEGKGKTKKESEQRAAESAYKQLKQIK"
     gene            400..3966
                     /gene="smc"
                     /locus_tag="POH73_002011"
     CDS             400..3966
                     /gene="smc"
                     /locus_tag="POH73_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDI6887944.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVNDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLVERKKNQSETNARYEEEQENLIELLE
                     NISSEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEVKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIHGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRAVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETHLESIKASLKRLEDEIERYTKLS
                     KEGKESLTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQSQQTKHQLNDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEATLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAMFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            3966..5216
                     /gene="ftsY"
                     /locus_tag="POH73_002012"
     CDS             3966..5216
                     /gene="ftsY"
                     /locus_tag="POH73_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDI6887945.1"
                     /translation="MSFFKRLKDKFATNKENDEVKSLTEEQGQDKLEDTHSEGSTQDV
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLISRYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGAK"
     gene            5203..5535
                     /locus_tag="POH73_002013"
     CDS             5203..5535
                     /locus_tag="POH73_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDI6887946.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKKHLSNPEQIQRY
                     IQQLEDLE"
     gene            5561..6928
                     /gene="ffh"
                     /locus_tag="POH73_002014"
     CDS             5561..6928
                     /gene="ffh"
                     /locus_tag="POH73_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDI6887947.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTKLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            7363..7638
                     /gene="rpsP"
                     /locus_tag="POH73_002015"
     CDS             7363..7638
                     /gene="rpsP"
                     /locus_tag="POH73_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDI6887948.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            7826..8329
                     /gene="rimM"
                     /locus_tag="POH73_002016"
     CDS             7826..8329
                     /gene="rimM"
                     /locus_tag="POH73_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDI6887949.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            8329..9066
                     /gene="trmD"
                     /locus_tag="POH73_002017"
     CDS             8329..9066
                     /gene="trmD"
                     /locus_tag="POH73_002017"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDI6887950.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQARVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            9169..9519
                     /gene="rplS"
                     /locus_tag="POH73_002018"
     CDS             9169..9519
                     /gene="rplS"
                     /locus_tag="POH73_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDI6887951.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(9763..>10847)
                     /gene="yfhO"
                     /locus_tag="POH73_002019"
     CDS             complement(9763..>10847)
                     /gene="yfhO"
                     /locus_tag="POH73_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HDI6887952.1"
                     /translation="YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQ
                     NLLSLWNVNDRIRVNHDDNLPYGFKINSEHKDNKVRWIHSKNTIHYPSAHITNKVFSN
                     KELKSPLDKEQAMLQGIVSNNTKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQV
                     KQNNGGLTVQLPKSVSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRF
                     VTPVTIRIKASDRIRLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVRKTKQGYT
                     ITKNKNSSGYIVLPTAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPP
                     YYYLLITITIFGIICSIIFTRWARQK"
BASE COUNT         4066 a         1471 c         2071 g         3239 t
ORIGIN      
        1 acatgtagaa caaaatgagt tattaggcgt ggtagatttt aaaacacaat tccaagaata
       61 tgtgcaccag caaaataaag gtgatgtaac ctataattta ataaaagaag agggaccggc
      121 acatcatcgt ctattcactt cagaagttat tctgcaaggg gaagcaatag ctgaaggtaa
      181 agggaaaacg aaaaaagaat cagaacaacg cgctgctgaa agtgcctata agcaattaaa
      241 gcaaattaaa tagaaattta tatgatacga catataggtg ttcgatttag gatttatgga
      301 agaattttta atattttatg gaacaacttc gaaatttaat atacaaatac taaaatatcg
      361 tatacatgtg ttctttaaaa ttgtgataag gagtttagga tggtttattt aaaatcaata
      421 gatgccattg gatttaagtc ttttgcagat caaaccaatg ttcaattcga taaaggtgtg
      481 actgcaattg ttggtccaaa tggaagcggt aaaagtaata ttacagatgc tattaaatgg
      541 gtgttgggtg aacaatcggc taaatcatta cgtggctcaa aaatggaaga tattatcttc
      601 tcaggtgcag aacatcgcaa agctcaaaat tatgctgaag tacagttaag attagataat
      661 cattctaaaa agctcagtgt tgatgaaaac gaagttattg taacaagaag attgtatcga
      721 agtggtgaaa gtgagtacta cataaataat gaccgtgcaa gattaaaaga tattgccgat
      781 ttatttttag attctggatt ggggaaagaa gcatatagca ttatctcgca aggtagagtt
      841 gatgaaatac taaatgctaa accaattgat agacgtcaaa ttattgaaga atcggctggt
      901 gtacttaaat ataaaaaacg taaagctgaa tcattaaata aacttgacca aacagaagat
      961 aatttaacga gagtagaaga cattttatat gatttggaag gtcgcgtaga acctctaaaa
     1021 gaggaggcag ctatagctaa agaatataag acactttcac accaaatgaa acatagtgac
     1081 attgtagtta cagtgaacga tattgatcaa tatacaaatg acaatagaca attagatcaa
     1141 cgtttaaatg atttacaagg tcaacaagca aataaagaag ctgacaagca acgtttaagc
     1201 caacaaattc aacaatataa aggtaagcgt catcaacttg ataatgatgt tgaatcgctt
     1261 aattatcaat tagtaaaagc tacggaagcc tttgaaaaat atacgggaca attaaatgtt
     1321 ttagtagaac gtaagaaaaa tcaatctgaa acaaatgcac gatatgaaga agaacaagaa
     1381 aatttaattg agcttttaga aaatatatca agtgagattt ctgaagctca agatacttat
     1441 aagtctctga aaagtaaaca aaaagaactc aatgctgtca ttcgtgaact tgaagaacaa
     1501 ctatatgttt cagacgaagc acacgatgaa aaattggaag aaattaaaaa cgaatactat
     1561 acattaatgt cagagcaatc agatgttaac aatgatattc gttttttaaa gcatactata
     1621 gaagagaatg aggttaaaaa atcaagacta gattctcgat tagttgaagt ctttgagcaa
     1681 ttgaaagata ttcagggtca aataaaaacg actaaaaaag aatatcaaca gaccaacaaa
     1741 gaactttctg ctgtagataa agaaattaaa aatatagaaa aagacctcac tgatacaaaa
     1801 aaagcacaaa acgaatacga agagaaattg tatcaagcat atcgatatac cgaaaaaatg
     1861 aaaacacgta ttgatagttt ggcaacgcaa gaggaagaat atacttattt tttcaatggc
     1921 gtcaaacata ttttgaaagc taaaaataaa gaattaaagg gtattcatgg tgcagttgcg
     1981 gaaattattg atgtgccatc taaattaact caggcaattg aaacagcatt aggtgcttca
     2041 ttacaacatg tcattgtaga ttcagaaaaa gatggacgcc aggctattca atttttaaaa
     2101 gaacgtaatt taggtcgtgc gacgttttta ccattaaatg ttatacagag tagagcggta
     2161 gcgactgata ttaagtctat tgctaaagag gcaaacggat ttattagtat tgcttcggaa
     2221 gcagttaaag tagcaccaga atatcaaaat attatcggga atttattagg taatacgatt
     2281 atcgttgatc atttaaagca tgcaaatgaa ttggcacgtg cgattaaata tcgaactcgt
     2341 attgttactt tggaaggtga tattgtaaat cctggtggtt ctatgactgg tggtggcgct
     2401 cgtaagtcaa aaagtattct gtctcaaaaa gacgagttga caacaatgag acaccaatta
     2461 gaagattact tgcgtcaaac agaatcattt gaacaacaat ttaaagagtt gaagataaaa
     2521 agtgatcaat taagtgaact gtattttgaa aaaagtcaaa agcataatac acttaaagag
     2581 caagtgcatc attttgaaat ggagctcgat agattaacta cacaagaaac acaaataaaa
     2641 aatgatcatg aagaatttga atttgaaaaa aatgatggtt atacgagtga caaaagtcga
     2701 caaactttga gtgaaaaaga aactcattta gaaagtatta aagcatcttt aaaacgacta
     2761 gaagatgaaa ttgaacgcta cacaaaactt tctaaagaag gtaaggaaag cttaactaaa
     2821 acacaacaaa cgttacatca gaaacaatct gatcttgctg tggttaaaga gcgtattaaa
     2881 acacaacaac agacaataga tcgattaaat aatcaaagtc aacaaaccaa acatcaatta
     2941 aatgatgtta aagaaaaaat agcattcttt aattcggatg aagtgatggg cgaacaagct
     3001 tttcaaaata ttaaagatca aattaatggt caacaagaaa cgagaacacg cttatctgat
     3061 gaattagata aattgaaaca acaacgtatt gagttgaatg aacaaatcga tgcgcaagaa
     3121 gctacactac aagtttgtca ccaagatatt ttagctatcg aaaatcacta ccaagatatt
     3181 aaagctgaac aatcaaagct agatgtatta attcatcatg cgatagatca tttaaatgat
     3241 gaatatcaat tgactgttga acgtgcaaaa tctgaatata cgagtgatga atcgattgac
     3301 gcattacgta aaaaagttaa attaatgaag atgtcgattg atgaactagg tcctgtaaac
     3361 ttaaatgcaa ttgaacaatt tgaagagtta aatgaacgtt atacattttt aagtgagcaa
     3421 cgtacagatc ttcgtaaagc taaagaaaca ttagagcaaa ttataagtga aatggatcaa
     3481 gaggttactg aaagatttaa agaaactttc catgctattc aaggacattt tacagctatg
     3541 ttcaaacaat tgtttggtgg aggcgatgca gaattgcaat taactgaagc cgattattta
     3601 acagctggta ttgatattgt ggtacaacca ccgggtaaaa agttgcaaca tttatcgtta
     3661 ctaagtggtg gtgagcgtgc attaactgct attgctttac tatttgcaat tttaaaagta
     3721 agatctgcac cttttgttat attagatgag gttgaagctg cattagatga agcgaatgtt
     3781 attagatacg caaaatattt aaatgagtta tcagacgaaa cacaattcat tgttattaca
     3841 caccgtaaag gaacaatgga atttgcagat aggttatacg gtgtaacaat gcaagaatca
     3901 ggtgttacta aacttgtgag tgtgaattta aatacaatag atgatgtgtt gaaggaggag
     3961 caataatgag cttttttaaa cgcttaaaag ataagtttgc aacaaataaa gaaaatgatg
     4021 aagttaaatc cttaacagaa gaacaaggtc aagacaaatt agaagataca cattctgaag
     4081 gttcaacgca ggacgtaaat gatttagcag aaaatgctga agtgaaaaag aagccacgca
     4141 agttgagtga agcggatttt gatgacgatg gcttaatatc aattgaagat tttgaagaaa
     4201 ttgaagctca aaaaatgggt gctaaattta aagcaggact cgaaaaatct cgtcaaaatt
     4261 tccaagaaca attaaataat ttgatatcga gatatcgtaa agtagatgaa gacttttttg
     4321 aagctttaga agaaatgtta atcactgcag acgtcggttt taatacagtg atgacgttaa
     4381 ctgaagaatt acgtatggaa gcacaacgac gtaatattca agatactgaa gatttgcgtg
     4441 aagtcattgt tgaaaaaatc gtagagattt accatcaaga agatgataat tcagaagcta
     4501 tgaacttaga agatggtcgt ttaaatgtca ttttaatggt tggtgtgaat ggtgttggta
     4561 aaacaacaac aattggaaaa ttagcttacc gatataaaat ggaaggtaaa aaagtaatgt
     4621 tagctgcggg cgatactttt agagcgggtg ctattgatca attgaaagtt tggggcgaac
     4681 gtgttggtgt agatgtaatt agccaaagtg aaggttctga tccagctgct gttatgtatg
     4741 atgcgattaa tgccgctaaa aacaaaggtg ttgatatttt aatctgtgat accgctggac
     4801 gtttacaaaa taaaacaaat ctaatgcaag aattagaaaa agttaagcgt gtaattaatc
     4861 gagcagtgcc agatgcgcct catgaagcat tactatgttt agatgctaca actggtcaga
     4921 atgcgttgtc acaagctaga aactttaaag aagtaacaaa tgttacaggt attgtattaa
     4981 cgaaattaga tggtacagcc aaaggtggta tcgtattagc cattcgtaat gaattgcaca
     5041 ttccagttaa atatgtaggt ttaggtgagc aattagatga cttacaacca tttaaccctg
     5101 aaagttatgt ctacggctta ttcgctgata tgattgaaca aaatgaagaa ataacaacag
     5161 ttgaaaatga tcaaattgta acagaagaaa aggacgataa tcatggggca aaatgattta
     5221 gttaaaacgt tacgaatgaa ttatttgttt gatttttatc aatccttatt gacgaataaa
     5281 caacgtaatt atttggaatt attttatctc gaagattatt ctttaagtga aatcgcagat
     5341 acttttaatg tgagtagaca agcagtttat gataatataa gaagaactgg cgatttagtt
     5401 gaagattatg aaaagaaatt ggaattatac cagaaatttg agcaacgccg agaaatatat
     5461 gatgaaatga aaaaacattt aagtaatcca gaacaaatac aacgttatat tcaacaatta
     5521 gaagacttag aatagatata aattgaggga ggacatcgat atggcatttg aaggcttatc
     5581 agaacgcctg caagcgacga tgcaaaaaat gcgtggtaag ggtaaactta ctgaagctga
     5641 tataaagata atgatgcgtg aagtaagatt agcgttactt gaggctgacg taaactttaa
     5701 agtggtaaaa gaatttatta aaacagtatc agaacgcgca ttaggttccg atgtaatgca
     5761 atcattaaca ccagggcaac aagttattaa aatagttcaa gatgaattaa cgaagttgat
     5821 gggtggagaa aatacatcga ttaatatgtc aaataaacca cctactgttg ttatgatggt
     5881 tggtttacaa ggtgctggta aaacaacaac tgcaggtaaa ttagcattat tgatgcgtaa
     5941 aaaatacaac aaaaaaccaa tgttagttgc agcagatatt tatcgtccag cagcgataaa
     6001 tcaattacaa acagtaggaa aacaaattga tattcctgta tacagtgaag gggatcaagt
     6061 aaagccacaa caaattgtaa ctaatgcatt aaaacatgct aaagaagaac atttagactt
     6121 tgtaatcatt gatacagcag gtcgattaca catcgatgaa gcattgatga acgaattaaa
     6181 agaagtaaaa gacattgcta aaccaaacga aattatgtta gttgtcgatt caatgacggg
     6241 tcaagatgct gtcaatgttg cagaatcatt tgacgatcaa cttgatgtca caggtgttac
     6301 cttaactaaa ttagatggtg atacacgtgg tggtgcagct ttatctattc gttcggtgac
     6361 acaaaaacca attaaatttg ttggtatgag tgaaaagtta gatggtttag agctattcca
     6421 tcctgaacgt atggcatcac gtattttagg tatgggtgat gtgttaagtt taattgaaaa
     6481 agcacaacaa gatgtggatc aagaaaaagc aaaagattta gagaaaaaga tgcgtgagtc
     6541 atcatttact ttagatgatt ttttagaaca acttgatcag gtgaaaaatt taggaccact
     6601 ggatgatatt atgaaaatga ttccaggtat gaataaaatg aaagggctag ataagcttaa
     6661 tatgagtgaa aagcaaattg atcatattaa agcgattatc cagtcaatga cgccggctga
     6721 aagaaacaat ccagacacat tgaatgtatc acgtaaaaag cgtattgcta aaggttctgg
     6781 acgttcatta caagaagtca atcgtttgat gaaacaattt aacgatatga agaaaatgat
     6841 gaaacaattc actggtggcg gtaaaggtaa aaaaggtaaa cgcaatcaaa tgcaaaatat
     6901 gttaaaaggt atgaacttac cattttaatg tttaatttaa catgctgatt tttgttgcaa
     6961 tgagatgtgc atcccgaaag ttatactaaa gaatataact ttatggggtg catattttta
     7021 gatacacaat aataattaaa tactgaaatt gctgatttgc tagaacatga ggttattgtt
     7081 gctaatatta ataagttata ttgggagaac gtgtgcggta gaagtggtac cattcatgtc
     7141 cttacgggat aaattaacta aacacgtatg tggtgaaggc tactatgaat ttttataacc
     7201 tattaacagt ctaatttagt aatcacatag ttatttttgt aaggaaaagg ttcaataatt
     7261 ttctaggtaa agaaaaaact ctttacaaac attcacacac ctgttaatat tatttcttgt
     7321 agaaaataaa aattaaaaca tgacttaaag gagattttat aaatggcagt taaaattcgt
     7381 ttaacacgtt taggttcaaa aagaaatcca ttctatcgta tcgtagtagc agatgctcgt
     7441 tctccacgtg acggacgtat catcgaacaa atcggtactt ataacccaac gagcgctaat
     7501 gctccagaaa ttaaagttga cgaagcgtta gctttaaaat ggttaaatga tggtgcgaaa
     7561 ccaactgata cagttcacaa tatcttatca aaagaaggta ttatgaaaaa atttgacgaa
     7621 caaaagaaag ctaagtaatt tagcgtaaaa ttgttctaac aataagaata actcgtttac
     7681 actgacagtt attactcaat gatacgttgg gaatatcaca tgttagtaat atagaacgta
     7741 ttgggtacca taatggtgcc ctttttcttt gaattatttt caattaaaat agaagtgttc
     7801 aaagcataga gttggaggta atagaatgag agttgaagtt ggtcaaattg ttaacacaca
     7861 tggtattaaa ggtgaaatta aagtaaaatc caattcagac tttacagacg ttcgttttca
     7921 acccggtcaa gtgctgacag ttgtgcataa caataacgac cttgaatata ctgttaagtc
     7981 acatagagtg cataaagggc ttcatatgct cacatttgaa ggtattaata atattaatga
     8041 tattgagcat ttaaaaggga gttctattta tcaagagcgt gatcatgaag atatcgtact
     8101 tgaggaaaat gaattttatt attcagatat tataggatgt acagtttttg atgatcaaga
     8161 aacaccaata ggtcgtgtaa ttaatatatt tgaaacaggt gcgaatgatg tgtgggtgat
     8221 taaaggatct aaagaatatt tgattcctta tattgctgat gttgtaaaag aagtggatgt
     8281 tgaaaataaa aaaattatca tcacgccaat ggaaggattg ttggattaat gaaaattgat
     8341 tatttaactt tatttcctga aatgtttgat ggtgttttaa atcattcaat tatgaaacgt
     8401 gcccaagaaa acaataaatt acaaatcaat acggttaatt ttagagatta tgcaattaac
     8461 aagcacaacc aagtagatga ttatccgtat ggtggcggac aaggtatggt gttaaagcct
     8521 gaacctgttt ttaatgcgat ggaagactta gatgtcacag aacaagcacg cgttatttta
     8581 atgtgtccac aaggcgagcc attttcacat cagaaagctg ttgaattaag caaggctgac
     8641 cacatcgttt tcatatgcgg acattatgaa ggttacgatg aacgtatccg aacacatctt
     8701 gtcacagatg aaatatcaat gggtgactat gttttaacag gtggagaatt gccagcgatg
     8761 accatgactg atgctattgt tagactgatt ccaggtgttt taggtaatga acagtcacat
     8821 caagatgatt cattttctga tgggttatta gagtttccgc aatatacacg tccgcgtgaa
     8881 tttaagggtc taacagttcc agatgtttta ttgtctggaa atcatgccaa tattgatgca
     8941 tggagacatg agcaaaagtt gatccgcaca tataataaaa gacctgactt aattgaaaaa
     9001 tatccattaa ctaatgcaga taagcaaata ttagaaagat ataaaatagg attgaaaaaa
     9061 ggttagtatt gtgctaacat aatctgagtg tggaaacaca aaaatcacta tgatccgctg
     9121 ctatatattt gtcgaggcaa gaacataggt tgaagaggag aatttataat gacaaatcac
     9181 aaattaatcg aagcagtaac taaatcacaa ttacgtacag acttaccaag tttccgtcct
     9241 ggtgatactt tacgtgtaca cgtacgtatc attgagggta ctcgtgagcg tatccaagta
     9301 ttcgaaggcg ttgtaattaa acgtcgtggc ggtggcgttt ctgaaacgtt tacagttcgt
     9361 aaaatttcat caggtgttgg cgtggaacgt acattcccat tacacacacc aaaaattgaa
     9421 aaaatcgaag ttaaacgtcg tggtaaagta cgtcgtgcta aattatatta cttacgtagt
     9481 ttacgtggta aagctgctag aatccaagaa attcgttaat cagcatttaa acaaagctat
     9541 gtatgagtca aattcgactc aatcaataaa gccatctagg tcacttttat ggagtgatat
     9601 agatggcttt ttttgctgtt ttattaatgt aaagttgata atgtaattgt ttttcccttc
     9661 tttattcata acgtgtttgc tcaaaatggt tatataaaca aaaatctact aagtgccatg
     9721 aaaaaggact tagtagattt attaaatatg tgcgtagcaa cattattttt gtcttgccca
     9781 tctcgtgaaa ataatactac atataatgcc aaatatagta attgttataa gtaaataata
     9841 gtatggtggg gtatagctca attgaatctt tgttatattt ttaggtgctt taatgccggt
     9901 cataacacca tttacttgtt caactttaag actttgatca cctgatgtcg ctttcatacc
     9961 ttgattatat gctgttggca aaacaatata cccagatgaa tttttatttt tagtaatagt
    10021 ataaccttgc tttgtctttc taactttgac agcctctaat gaatttgaag cgtctttaag
    10081 cgtggtataa tcttcgccgt atatcccttt taaatttaca cgatacttac ctttaggcaa
    10141 tgataatcta attctatctg aagctttaat gcgtatcgtt acgggtgtta caaagcgtcg
    10201 atatttataa gtgagtttat ttctttcttg tgtatattca ttcactttaa catcatgagc
    10261 tttatccggc gaaagtaatt ctaaatccat ttcaaaatac aaatctttaa attgattaga
    10321 aactgatttt ggcaactgta cagttagacc accattattt tgtttaactt gtaataaatg
    10381 ttttgtagga gattgccagg ctgcactatt taatttaatt gttgaatctg atagtaaatt
    10441 tttattggct ttaaaatgtg tattaacatc tttagtattg ttagaaacaa tcccttgcaa
    10501 cattgcttgt tctttatcta atggagattt taattcttta ttggaaaaga ccttatttgt
    10561 aatatgtgca cttggataat ggatggtatt tttagaatga atccaacgaa ctttattgtc
    10621 tttgtgttca gagttaattt taaatccata tggtaagttg tcatcatgat tcactctaat
    10681 tcgatcatta acattccaaa gtgatagtaa attttgacga ttgccaagta gtctataagt
    10741 gctgttttta tcgattggca tattaatttg gagtgtcttg tcataatatt ttaaaatgtc
    10801 tccattaaaa atactagaat ataatgaaat gccattataa tgatata
//
LOCUS       DAMZPP010000105        10693 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMZPP010000105 DAMZPP010000000
VERSION     DAMZPP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10693
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.105"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..3160)
                     /gene="sasC"
                     /locus_tag="POH73_002020"
     CDS             complement(<1..3160)
                     /gene="sasC"
                     /locus_tag="POH73_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HDI6887953.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDHNV
                     QGGSNQALPGNSQNTNADTNRDIVNDSQNTPNAHATDNTSTNQALTNHQNVDVANQVG
                     PAPIQPSASPAQNNNNSNANSTATEPAANTNNNLASNNNTLNVPNNTDNNDSARHLTL
                     KEIQEDVRHSSDKPELVAIAEEASNRPKKRSRRAAPTDPNATPADPTATPADPTAGNG
                     SAPVAITAPYTPTTDPNANNIGQNAPNEVLSFDDNNIRPSTNRSVPTVTVVDNLPGYT
                     LINGGKVGVFSHAMVRTSMFDSGDAKNYQAQGNVIALGRIRGNDTNDHGDFNGIEKTL
                     TVNPNSELIFEFNTMTTKNYQGMTNLIIKNADNDTVIGEKVVAYGPIWRLLKVPENVS
                     HLKIQFVPKNDAITDARGIYQLRDGYKYYDFVDSIGLHSGSHVYVERRTMEPTATNNK
                     EFTVTTSLKNNGNFGASFNTDDFVYKIQLPEGVEYVNNSLTKDFPSGNSGVDINDMNV
                     TYDAANRIITIKSTGGGTGNSPARLMPDKILDLKYKLRVNNVPTPRTVTFNDTLTYKT
                     YSQDFINSPAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNDLKRR
                     AQTILDENRNNVPLNKRVSQADIDSLANQMQHTLIRSVDAENAVNRKVDDMEDLVNQN
                     DELTDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPN
                     AKQAIRDKAAKQREIINHTPDATQDEIQDALNQLTTDETDAIDNVTNATTNADVETAK
                     NNGINTIGAVAPQVTHKQAARDAINQATATKRQQINSNREATQEEKNAALNELTQATN
                     HALEQINQATTNDDVDTAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANP
                     DATQEERQAAIEKVNAAVAVANTNILNANTNADVEQVKTNAIQGIQAIEPATKVKTDA
                     KNAIDQSAETQHNAIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKD
                     QGTQNIVVIQPATQVKTDARNAV"
     gene            complement(3484..3795)
                     /locus_tag="POH73_002021"
     CDS             complement(3484..3795)
                     /locus_tag="POH73_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDI6887954.1"
                     /translation="MKSITTDELKTKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNAFNKKETYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
     gene            complement(3817..6234)
                     /locus_tag="POH73_002022"
     CDS             complement(3817..6234)
                     /locus_tag="POH73_002022"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDI6887955.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNSEGKVEVFTTRPDTIYGASFLVLSPEHALVDSITTDEYK
                     DQVKAYQTEASKKSDLERTDLAKDKSGVFTGAYAINPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEKKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLGIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            complement(6523..7704)
                     /locus_tag="POH73_002023"
     CDS             complement(6523..7704)
                     /locus_tag="POH73_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6887956.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMVLLVFALILLMVFKENNTQPKKID
                     A"
     gene            7815..8768
                     /locus_tag="POH73_002024"
     CDS             7815..8768
                     /locus_tag="POH73_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDI6887957.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMQTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            8765..9328
                     /locus_tag="POH73_002025"
     CDS             8765..9328
                     /locus_tag="POH73_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6887958.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLVKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINALLSLMSTEGIIVLVIYHGHSEGQLEKQALLDYLSTLEQ
                     KHAQVLKYQFLNQRNHAPFICAIEKIS"
     gene            complement(9447..9848)
                     /locus_tag="POH73_002026"
     CDS             complement(9447..9848)
                     /locus_tag="POH73_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6887959.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(10421..>10693)
                     /locus_tag="POH73_002027"
     CDS             complement(10421..>10693)
                     /locus_tag="POH73_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6887960.1"
                     /translation="DTMAHIHDIQPMPTLLMYGTKDLIVETRAIDEFKEKYQTPELYF
                     KAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
BASE COUNT         3277 a         2009 c         1614 g         3793 t
ORIGIN      
        1 ttacagcatt gcgagcatcc gttttaactt gtgttgccgg ttgaatcaca actatatttt
       61 gtgtgccctg atcttttgct tgtgcaactt cttgattcgt atctgctgca ttaatatttt
      121 gcttcgctgt ggctacagct tgatcaagca attgttgtgc tgcttgttgc tcttctaagg
      181 tcgcatcatt attattaaat atcgcattat gttgcgtttc cgcactttga tcaatagcgt
      241 ttttagcatc tgttttaacc tttgtagctg gttcaatggc ttgtatacct tgaattgcat
      301 ttgtctttac ttgctcaaca tcagcattgg tattagcatt taatatatta gtattcgcaa
      361 cagctacagc agcatttact ttctctattg ctgcttgtct ttcttcttga gtcgcatcag
      421 gatttgcatt gatctctgcg atatgctgtt gtgcatcatg tgatacggca tctcttgctg
      481 cttgcttgac aacagttaca ggcgcaatag gattaatggc attcagacca tcacctttgg
      541 cagtatctac atcatcattg gttgtcgctt gattgatttg ttctaatgcg tggttcgtgg
      601 cttgcgttaa ttcattcaat gctgcatttt tctcttcttg tgttgcttct ctattgctat
      661 ttatttgttg tcgtttcgtt gctgtcgctt gattaattgc atctcttgca gcttgtttgt
      721 gtgtcacttg tggcgcaact gcaccaattg tattaatacc attattttta gctgtttcaa
      781 catcagcatt ggtagtagca ttcgtaacat tatcaatagc atctgtttca tccgttgtta
      841 attgatttaa tgcatcttga atttcatctt gagtagcatc tggcgtgtga ttgataattt
      901 ctctttgttt cgctgcttta tcacgtatag cttgtttagc atttggttta acaactggtg
      961 ttgcagtgtc tccacttaaa gtctgtatac cttgatcttt aattctagta acgccatcat
     1021 cagtcgtttg gtcaccaata ttcccaataa tttcattttt atgttcctcg atgacttgaa
     1081 tcgctgcttg tttttcttca tctgtcagtt catcattttg gttaactaaa tcttccatgt
     1141 catcaacttt tctattaacg gcattttcag cgtcaacact gcgaattaac gtatgttgca
     1201 tctgatttgc taatgaatcg atatctgctt gagaaactct tttgtttaaa ggtacattgt
     1261 tacggttttc atctaaaatt gtttgtgcgc gtcttttaag atcattaaaa atatctaatg
     1321 atgcaaatgt ataatccgct tgttgaattc gtctatcgac ttcggcttgc aatgcgtctt
     1381 tattcatgat gatatcaatt gtatatggat ttgtacttac agtatgactt tcagcaggtg
     1441 aattaataaa atcttgtgaa tatgttttat acgttaatgt atcgttaaat gttactgttc
     1501 ttggtgttgg cacattgttc acacgtagct tatacttcaa atccaatatt ttatcaggca
     1561 ttagtcgtgc cggcgaattc cctgtacctc caccagtact tttaattgta ataattcgat
     1621 ttgctgcgtc atacgtcaca ttcatatcat taatatcaac acctgaatta ccgctaggaa
     1681 aatctttagt caatgaatta tttacatatt caacaccttc aggtaattga attttatata
     1741 caaaatcatc tgtattgaat gaagcgccaa agttaccatt attctttaat gacgttgtaa
     1801 ctgtaaattc tttattattt gttgctgttg gctccattgt acgtctttca acatagacat
     1861 gtgacccaga atgaagaccg attgagtcta caaagtcata gtatttatat ccatctcgta
     1921 attgataaat accacgtgca tctgttattg cgtcattttt aggtacaaat tgaattttta
     1981 gatgactaac attttcaggt acttttaata agcgccaaat cggaccataa gcaactactt
     2041 tttcaccaat aacagtatcg ttatcagcat ttttaatgat taaatttgtc ataccttgat
     2101 agtttttagt agtcatagta ttaaattcaa agattaattc agaattcgga tttactgtta
     2161 atgttttctc gataccatta aaatcgccat gatcatttgt atcatttcct ctaatacgac
     2221 ccaatgcaat tacattgcct tgcgcttgat agttcttggc atctcctgaa tcaaacatgc
     2281 tcgttcttac cattgcatga ctaaacaccc ctactttacc accattaatc agtgtgtagc
     2341 ctggtaaatt atcaacaaca gttactgtag gcacagaacg gttcgtactt ggtctaatgt
     2401 tgttatcatc aaatgaaagc acttcgttag gtgcattttg tcctatatta ttggcattgg
     2461 gatcagttgt tggcgtgtat ggcgctgtaa ttgcaactgg tgcactaccg tttcctgccg
     2521 ttggatctgc tggtgtagcc gttggatctg ctggtgttgc attaggatct gttggcgcag
     2581 cacgtctgct tctctttttc ggtctattag atgcttcttc agcaatcgca actaactctg
     2641 gcttatcaga cgaatgacga acatcttctt gaatttcttt taaagtcaga tgacgcgctg
     2701 aatcattgtt atctgtatta ttaggcacgt ttaatgtatt gttatttgat gctaaattat
     2761 tatttgtatt cgccgctggc tctgttgctg ttgaattagc attagaatta ttattatttt
     2821 gcgcaggcga cgcgctaggc tgtattggag caggcccgac ttgatttgcc acatcaacgt
     2881 tttgatgatt agtcaatgct tgatttgttg atgtattgtc tgttgcatgt gcattaggag
     2941 tattttgcga atcatttact atgtctcgat tagtatcggc atttgtattt tgtgagttgc
     3001 caggtaatgc ttgatttgaa ccaccttgca cattatgatc cgtagttaaa gcttgtgcac
     3061 catttgggtt tgaaagtaat aaaactgtcc cgattaaagt agagaatatc cctactttat
     3121 attttctaat actatattta tttttcttta acaaattcat ttcgttttcc tcctggttgt
     3181 ctattctctg ctgtgttgtt tagaattatg ctaaaaatat aagctccccg attatgttta
     3241 taaccatatt attttgtaca acattttaag ttcactcaat tttatcatca tcattttata
     3301 aaataaagtt ttattttatt acaatttagt ttccgttttg taattaatca cttaaaaaat
     3361 cataacaaga acgctttaga ttgttttgta acagaaagca attcatagca aaaaacacca
     3421 agcaaccgaa acgttacttg gtgtcattac aaatgtaata ttattttaaa ttatgtcact
     3481 actttaaata ctttttattt ccaaaccttc atcgccccaa gcgtgcatgc cgccttcgac
     3541 atttacggca tcaatgccat ttgcctctaa atattctaca actttagcgc ttcgaactcc
     3601 accagcacat acaatatagt atgtctcttt tttgttaaat gcatttaaat tgtcgggaat
     3661 ggtatccatt ggaattaact ttgcattagg tatatatccc attgctgttt cttcgtcagt
     3721 acgaacatca acaatttgaa ctggtttaga ttctaaaagt ttggttttta attcatctgt
     3781 agtaattgac ttcattttaa agtcctcctt ataacattat ttagctacaa tattgactaa
     3841 tttttgagga acagcgatga ctttcatgat gtctttacct tcaatactcg ctttaacatt
     3901 gtcattagat aaggcaattt cttgcatttc ttcttttgat gtatctttag caattttaat
     3961 tttagctctc aatttaccat taacttgaac aacgatttct acttcatcat ctacaagtag
     4021 tgcttcgtca taagttggcc aaggttggta cgtaatagac tcttcgtgtc ctaattttga
     4081 ccataattct tcaccgatat gtggtgcaat aggtgctaac attttaacga agccttcaat
     4141 gtaaggttta taaacttcat caactttata acactcatta ataaatacca ttaattgact
     4201 aatagcagta ttaaatccta atgtttcaaa gtcttctgtt acctttttaa cagtttggtt
     4261 ataaacttta tctaaagatt tattatttgt agttactatt tttgaactta atgttccatc
     4321 ttcatttacc attaaacgcc atacacgatc taagaatcga cgagacccat ctaatccttt
     4381 ttcactccat gcaattgcag catctaaagg tcccataaac atttcgtaaa gacgtaaagt
     4441 atctgcccca tgagattgta ctatatcatc aggattgatt acatttcctt tagatttact
     4501 catcttctca ttaccttctc ctaaaatcat accttggtta aataattttt ggaaaggttc
     4561 ttttgtaggt acgataccta aatcataaag tactttatgc caaaatcttg catacaataa
     4621 gtgaagcact gcatgttcta cgccaccgat atataaatca acaggtaacc aatgttttaa
     4681 tttttcagga tctgctaaca tattttcatt tttaggatcg atgtaacgta aataatacca
     4741 acaactacct gcccattgtg gcattgtatt tgtttcacga cgccctttca tacctgtttt
     4801 ttcatctaca acatttacaa atgaatcaat attagctagt ggtgactcac cagtccctga
     4861 tggcttgatt tcatctgttt caggtaacaa caatggtagc tcttcttcag gaacagttgt
     4921 cattgttcca tcttcccaat gaatgacagg aattggttcg ccccaataac gctgacgact
     4981 gaataaccaa tctcttaatt tgtaattaac tttcttttcg ccagcacctt ttttctctaa
     5041 taattgaata gctttagtaa ttgccgcttc attttctaaa ccatcaagtt caccagaatt
     5101 aatatgttta ccttcaccag tgtatgccgc ttcttcaaca tttccacctt caatgacttc
     5161 aatgattggc aaatcaaact ttttagcaaa ttcataatct ctgtcatcat gcgctggtac
     5221 tgccataatt gcaccagtac catatgttga taatacataa tcagcaatcc aaatttgtac
     5281 tttttcacca gataaaggat taattgcata tgcaccagta aacacacctg atttatcttt
     5341 tgctaaatct gtacgttcta aatcagactt tttagaagct tctgtttgat acgctttcac
     5401 ttgatcttta tattcatcag ttgtaattga atcaaccaat gcatgctcag gacttaagac
     5461 taagaatgat gcaccataga ttgtatctgg tctagtcgta aatacttcta cttttccttc
     5521 cgaattatct acatcaaatg aaactttggc accttctgaa cgtccaatcc agttacgttg
     5581 catatctttt aaagactcag gccaatctaa atcatctaaa tctgctaata attgatctgc
     5641 atattctgta attttaagta cccattgttt cattggttta cgataaactg ggtgaccgcc
     5701 acgttcagat acaccgtcaa tcacttcttc gttagataag acagtaccta atgcaggaca
     5761 ccagttaact gcaacttcgt caacgtatgc taaacctttg ttatataact gtatgaaaat
     5821 ccactgtgtc catttatagt attctggatc tgttgtatta acttcacgat cccaatcata
     5881 actgaaccct aattctttaa tttgtcgttt aaaagtttgg atatttttct ttgtaaattc
     5941 acgtgggtcg ttaccagtat ctaaagcata ttgctctgct ggtaatccga atgcatccca
     6001 ccccatcgga tgtaatacat tatatccttg cattctctta tatcttgaaa tgatatctgt
     6061 tgctgtatag ccttcaggat gtccaacatg taaaccagca cctgatgggt atggaaacat
     6121 gtctaaagca taaaatttct tttgacctaa gttatcattt gttttaaatg ttttattttc
     6181 gtcccaatag tcttgccatt tcttttcaat ttgattgtgg ttgtaattca acacatttcc
     6241 tcctattcaa taaaaaaaga cacctctaac tattctattt acatagggac gacatttgcc
     6301 gcgttaccac cctatttcac aaaaattgtg cactcatttt aatagtaaga gatgtcattc
     6361 attcaattta aaattaatac gatttacatt tagtccttaa cactagctct cagcatcact
     6421 agctttctgt agaaaagctc caaacgaaat taaattatac aaattaatat tatgttataa
     6481 cgacatttat ttcaagtcta taacgttaat tctatttact cattatgcat ctattttttt
     6541 aggttgcgta ttattctcct tgaaaaccat taataatatt aatgcaaata caagtagtac
     6601 catcataccg ataaacatca tgcgcatatt aaatgcatca actaatacac caccaagaaa
     6661 tggaccgaat gcttttccta ctgtagcagc tgaattcaca aaaccttggt actgtccttg
     6721 cttaccatct ggcgctaact gattggctat agttggaact gcaggccata caaacatttc
     6781 tccaaaagtt aaaataatca taccgacaac aaatattgta aagttttcgg caaaactcgt
     6841 gacaaagaac gacaacataa aaatgatgat gccgacaaac atttgcttct ttaagtttcc
     6901 ttttaacaga tagagaattg gtttaattaa tggttgtgct actaaaatca ttattccgtt
     6961 aattgtccat aaaacactat attgtgccat tgaaatatta atcgattgtg taaatgatgc
     7021 gattgtagac tcccattgaa tatacgcaac ccaacaaatt gcaaacattg cacaaattag
     7081 tactaatgaa ataaatcttg ctttattctt tttaccagta atatctaaat gagttggata
     7141 tttaactttc gcattaattt caatattaaa ttgcgttacc gcgacaagtg caaacacaac
     7201 atacataata agattggcta aaaagatata gttaaagcta aattctgcga caaagccgcc
     7261 cattgcagcg ccgacagcaa caccaatatt ttgcgctaag tatatcgcat taaacgtttg
     7321 tcttccgcca ttcggccaca ctgctccagc cattgcgtat atcgcaggaa taatcattcc
     7381 gccaccaaag cctaacatta caagccatac agcataccaa ggccacccgt gaaagaaatt
     7441 aagtagcgtt gtactacaaa gacaagtgaa agttccaatt aaaatcgtct tgtatccacc
     7501 taatttatca aatagtgaac cacctaataa gtttccaata accatgccaa atgaatttat
     7561 cattagcact aaaccagcaa cagttaaact ttttccaagt tcttgtttca tataaattgt
     7621 atttaaaggc cacaaaaaac tggaaccagt aatatttaac gccatgccaa ttactagcca
     7681 ccagactgat ttaggtatat tcatatttca tagttcctct ctcttttttg tcaaactata
     7741 ataaacacta taacattaaa atatgttaaa atctgtactt agaactcttt tttttataga
     7801 aaggaaaatg acttatgggc aatcatttcc aatacgcttt tgaaaacaaa cgttatcaca
     7861 catggaatta ccatttaaaa aataaatttg gacaaaaaat atttaaagtt gcattggatg
     7921 gcgggtttga ctgtcctaat cgtgatggca ctgtagcaca tggtggatgt acattttgtt
     7981 ctgctgcagg tagtggagac tttgcaggta atcgtgcaga ttcaattgca gtacaattta
     8041 aagaaattaa ggaaaagatg catgagaaat ggcacgaagg aaaatatatt gcttattttc
     8101 aggcatttac aaatacacat gcaccagttg aagtgttaaa agaaaaattc gaacctgtac
     8161 ttaaagaacc gggtgttgtg ggattatcta ttggtacgcg tcctgactgt ctaccagacg
     8221 atgttgtcga atatttagca gatttgaatc aacgaacata tttatgggtt gaattgggac
     8281 tacaaacaat ccatcaatca acatctgatt taatcaatcg tgcccatgat atgcaaactt
     8341 attatgatgg cgtggcaaaa ttacgtaaac ataatatcaa tgtatgtaca cacatcatta
     8401 atggcttacc tggcgaagac tatgacatga tgatggctac tgccaaagaa gttgcacaaa
     8461 tggatgtaca aggtattaaa attcatttac ttcatttgtt aaaaggtaca ccgatggtaa
     8521 aacaatatga taaaggttta ttaactttta tgactcaaga agaatacaca aacctagttg
     8581 tagaccaatt agaagtgatt ccccctgaaa tgatcgttca ccgaattacc ggtgatggtc
     8641 caatagatat catggtcggt ccaatgtgga gtgttaataa atgggaagta ttaaatggca
     8701 tcgacgctga attagcacgt agaaattctt atcaaggctt gcgttacaag tctaaggtga
     8761 agcaatgaaa ttagaacgaa tactcccctt ttcaaaaaca cttattaaac aacatataac
     8821 accagaaagt attgttgtag acgcaacttg cggtaacggc aatgacactt tatttttagc
     8881 cgaacaagta ccagaaggac atgtttatgg tttcgacatt caagatttag ctttggaaaa
     8941 tacacgtgat aaagttaagg atttcaatca tgtttcttta gtaaaagatg gacatgaaaa
     9001 tattgaacat catataaatg atgcacataa aggtcatatt gatgcagcca tttttaacct
     9061 aggttatttg cctaaaggtg ataaatctat cgtgacaaag cctgacacga caatccaagc
     9121 tattaatgcg ttattatctt taatgtcaac cgaaggtatt atcgtattag ttatctatca
     9181 tggtcatagt gaaggccaac ttgaaaagca agctttgctt gattatttga gtactttgga
     9241 gcaaaagcat gcgcaagttt tgaaatatca atttttaaac caacgtaatc atgctccatt
     9301 catttgtgcc atagaaaaaa tttcttaaac atttgctgcg ctatactttt ttataattta
     9361 ggattgagta gctaacattt gaaaaaaata tcaacgacaa aaagaggttt tcaacttaat
     9421 gaaaacctct ttttatgcta ttaaacttac acagcaataa ttgcgtttaa actattttgc
     9481 attgctgttg ctctacttgc aatcgcatca ctgatgaaat tcaacaactc tactttctct
     9541 tgttgtaact tttcattgaa atgaataata actgttcttt catcgtcaac aggacgctct
     9601 ttgtaaatcc attctaattc aactaaatta ttatacgttc tagtacgctt atacggttta
     9661 acttcaacaa atctgtccat ttctttaagc gtcatagaac ctttttgcca taaagttagt
     9721 aaaattaaaa tttcttctct agacattttg tactcgcttt caatctcgct gaaaattgag
     9781 ttaatgtcac ccaaaagtgt ttctaattgc aaaatcccaa atacagtatc gttatttact
     9841 tttttcatgc tatacatctc ccaattaata atcacatatt tagctaccat acaagtatta
     9901 acaaacatcc caacaatccc aaaacttata tgtgctaact tatgcataaa actacaagtg
     9961 taaataaact tgctttctat tcaattgtct aagttgaata aaattaaata agtataaaaa
    10021 ccaataatca ctagagttaa tatttagaaa taagataata gtacataaaa caaatcactc
    10081 tattacatat taattttata atttatatac aaagaaaata tatcaatatt accattaaat
    10141 tgcaacaatt ctacatatat ttcctcaaaa aagatacgca ttgaattttt attacatata
    10201 actctcatga aaattgatta aactaaatta attagttacc tttaaattta acaaattaaa
    10261 tcaaaccgaa tgagactaaa ttggttattt ttcaagatat gatttgcata aatcaaaaca
    10321 tttcttacaa atcaaatgtg tataaaatgt gatatacatt ctcaacctta aaaaagttta
    10381 aaaaaaagat gagcatctac tgtctcacct ttttagaata ttaaatttct acaatttcgt
    10441 catcttcaac aataaagccc attgtattga cgctattatt taagaaagtc agaatataac
    10501 gcattacttc atcgcgttct ggctcattgt gaacctcgtg gtaaaaacct tgccaagctt
    10561 taaaatataa ttcaggtgtt tgatattttt ctttaaactc atcaattgcc ctagtttcaa
    10621 caattaaatc cttcgttcca tacattaata gtgttggcat tggttgaatg tcatgaatat
    10681 gagccatcgt atc
//
LOCUS       DAMZPP010000106        10688 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMZPP010000106 DAMZPP010000000
VERSION     DAMZPP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10688
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.106"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..193)
                     /locus_tag="POH73_002028"
     CDS             complement(<1..193)
                     /locus_tag="POH73_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDI6887961.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGV"
     gene            complement(186..1742)
                     /locus_tag="POH73_002029"
     CDS             complement(186..1742)
                     /locus_tag="POH73_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI6887962.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYIANTPRLSEIKKD
                     SRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            complement(1739..2707)
                     /locus_tag="POH73_002030"
     CDS             complement(1739..2707)
                     /locus_tag="POH73_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDI6887963.1"
                     /translation="MKSKIYILLLFLIFLSACTNTRHSESDKNVLTVYSPYQSNLIRP
                     ILNDFEKQEHVKIEIKHGSTQVLLSNLHNEDLSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIQGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQDHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            3295..5544
                     /gene="pflB"
                     /locus_tag="POH73_002031"
     CDS             3295..5544
                     /gene="pflB"
                     /locus_tag="POH73_002031"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDI6887964.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            5567..6322
                     /gene="pflA"
                     /locus_tag="POH73_002032"
     CDS             5567..6322
                     /gene="pflA"
                     /locus_tag="POH73_002032"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDI6887965.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            6426..6593
                     /locus_tag="POH73_002033"
     CDS             6426..6593
                     /locus_tag="POH73_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6887966.1"
                     /translation="MRQVQQWLKEDAFVSSYADKECDALLKMGFQYHRMGYYLLLAYE
                     YESFQIFIDPE"
     gene            6644..8407
                     /locus_tag="POH73_002034"
     CDS             6644..8407
                     /locus_tag="POH73_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HDI6887967.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWMEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDRFLVSSILFVVLKSSLFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDVVGLKMRQNGHEAKLVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHSENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(8571..8915)
                     /locus_tag="POH73_002035"
     CDS             complement(8571..8915)
                     /locus_tag="POH73_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HDI6887968.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSHLNLDAYQKRDILAAHYIAKSAIRTKNLDQMTKAKHRLESIY
                     DSISNPLHSQNN"
     gene            9105..>10688
                     /gene="coa"
                     /locus_tag="POH73_002036"
     CDS             9105..>10688
                     /gene="coa"
                     /locus_tag="POH73_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009557200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HDI6887969.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSKESRVNENSKY
                     DTPIPDWYLGSILNRLGDQIYYAKELTNKYEYGEKEYKQAIDKLMTRVLGEDHYLLEK
                     KKAQYEAYKKWFEKHKSENPHSSLKKIKFDDFDLYRLTKKEYNELHQSLKEAVDEFNS
                     EVKNIQSKQKDLLPYDEATENRVTNGIYDFVCEIDTLYAAYFNHSQYGHNAKELRAKL
                     DIILGDAKDPVRITNERIRKEMMDDLNSIIDDFFMDTNMNRPLNITKFNPNIHDYTNK
                     PENRDNFDKLVKETREAIANADESWKTRTVKNYGESETKSPVVKEEKKVEEPQLPKVG
                     NQQEDKITVGTTEEAPLPIAQPLVKIPQGTIQGEIVKGPEYLTMENKTLQGEIVQGPD
                     FPTMEQNRPSLSDNYTQPTTPNPILKGIEGNSTKLEIKPQGTESTLKGTQGESSDIEV
                     KPQATETTEASHYPARPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETN
                     AYNVTTNQDGTVSYGARPTQ"
BASE COUNT         3746 a         1632 c         1914 g         3396 t
ORIGIN      
        1 cgacgccatt tttacgtggc atacgaatat cagtaattac taattctggc tgatgttgtt
       61 gaattagtga taatgcttca acaccatctt tagcagtgta tattgtattg aaatgatagt
      121 ctccccaagg aatgatttgc tttaaacctt ctcgaataat tcgctcatca tcacaaataa
      181 ctaccttaaa catctacatt cccccttgaa agtggtattt tataacaaat taacgtacct
      241 tgattacgct ttgaaaaaat atggagtcgt gcatgtgaac catattgaat cattgcttta
      301 ttgtgtaaat gatttaatcc caaatgctta gtatcaaata catcattatt aagagattgg
      361 cgtacatatt gcaggcgaga tgacgacatc ccaataccat tgtcgcaaac taaaacatgt
      421 aaattctgac gtgccaatgt caggcgtata gtaatgtcca atgactcagt atctctacca
      481 tgtttaatag cattttctat gagtggctga agcatcattt taccaattgt ctggtgacgc
      541 gcttcttcag aactttcgat atggagctta atcatgtcat caaaacggat gttttgtatc
      601 gcaacatact gttcaatgta gttcaactct tcgtttaatt ccactgtatg tgagtttgta
      661 cgtaatgagt aacgtaacat ttgcgataat tgttggacca cagtttgtgc taatttcgga
      721 gataacgtaa ttaaatattg tattgtttgc atcgtattga ataggaaatg aggttggaat
      781 tggcgttcta tttcctttaa ctgaatatca cgcaagcgac gttctgtatg ctcgatagaa
      841 tggatcagtt gctcatttga ttcaaataaa tcgtaaatat aattattaat ttcttctagt
      901 tcactgttgt tttttaaagg cgtatatgta cctagatgac gatttttggc atagtaaatt
      961 ttttgaataa tcgtttcgat atcttttgtt tgtcgtttag ccatattatc tgcactaatg
     1021 aaaccaaata ttactagtaa aacaagaact acggccataa caattaacaa cgtgatacca
     1081 tcttcaatgt tttcatgtat atctttataa ataatgagac gatggtcagc atggtttaat
     1141 tttacagatt cattcataaa tccgaattgt tgcggtctat acttttcacc tatagtaaaa
     1201 cggtcatcgt tggcgtataa aatattgtca tattgatcaa cgataagtgc gaattgtcgg
     1261 ctatctttct taatttcact taaacgtggg gtgttagcta tataaatttt aagcatatat
     1321 gtactatttt tgaatttaag ctgatgcgtt gaaaataaat acatattttt agtgtttaaa
     1381 tgttcataat tattggttat aaactgattt ggtccagata attcataata aagtgttgcg
     1441 ggctgttggt gtattaattt taataattca cgttttgtag cggtaacatc atgatgattt
     1501 gttaaatcga gctcttgaaa cgaattatta tgctgtgtaa taaacgtctg aatttgcttt
     1561 tcagtatgat gtaaagatga ctgactttca tcaacatgtt gatgaatcgt acgatgctca
     1621 atccaaatat agatggcata gaagcttact agtccaataa taatcactaa aaatactgga
     1681 aaaatagtag acgcaaataa cgatcgtctc aattgatgtc tataaggttt gtatgccgtc
     1741 attgaatcat ctccaaaaat ttatgatgcg gaatgtccgg taatttagat ttcggtatta
     1801 aaggtatgtt cttaagattt tcgatagact gatcgctttg ttcactaaca tcttttcgaa
     1861 ttgacttggc atcgaactct gcaactaatc gttgttgtac tgagcggctt gttaaatatt
     1921 gcactaactt tttacgctta ggatgagggt gtgcattttt aactaaagca ataccatcaa
     1981 catttaacat tgttccttca attggataaa cgattgatac aggataacct ttgtttttcc
     2041 atgtgcgtgc atcttgttcg tagcttagac ctgcataata tttacctttt gcaacatctt
     2101 caatgacttt agacgtcttt gacagttgca tcgcgtggtc ttggaattga tgcacatcac
     2161 ttactcgatg atgcatgcta taaatagcgc gcatatgttg atagcctgtc gttgttgtat
     2221 ttggatttga gtacgcaatt ttacctttaa gtataggttg taataaatct tgataacctt
     2281 gaatcttaat atctccttgt aaatctgaat taactactat aactgttggc attaatagaa
     2341 agctagtaac atatttattg ttcgagcgat aatcctctaa ttgctgtgtt acagatgtat
     2401 cttgataggg aacaaaatct tctggatgat caattgtttc tgacaacaca ccacccataa
     2461 agacatcacc acgctccgaa agatcttcat tatgcaagtt tgaaagcagt acttgagtag
     2521 atccgtgttt aatttcaatt ttgacatgct cttgtttttc aaaatcattt aaaattggac
     2581 gaatcaagtt tgattgatac ggagaataaa ctgttaatac atttttatct gattcagagt
     2641 gacgcgtatt agtgcatgct gataaaaaaa tgagaaataa tagcaagata taaatttttg
     2701 atttcatgat atcccatcaa ttctatgtat attttaatac aataatttta gcaataaatg
     2761 acgcataagt aatgttaaat atttagaaat gtttatagat gacttgttaa gacgttgcaa
     2821 atgttgtgat agcacataat ttttgtttgt caagacgact taccgaagct gtaaagtcaa
     2881 actgttatat tttatttgaa gctgttatat aaaaattggc aagatattga acggttcaaa
     2941 agtaaatttt tacgtcaata aaagtattta atcctgtctc ttcatatata aaagtaaatc
     3001 tttctaagta ttgatttaac gcttattaac aatcattttt tataaacaaa tatatactcc
     3061 taaattaact tttaaagtaa tgaaaatagt gaacattata actgttgtgt aacagaatgc
     3121 aattagcata ttactgttac acaaattagt acagtttcta tgttttgaca tacatttgat
     3181 gaaaattgta cataatttat gtgaaaaaaa tcacaacaaa catgctacaa tgactatgaa
     3241 aacgttaaca tagcatttca aattcacaac attatacaga tggaggcgtt tagtatgtta
     3301 gaaacaaata aaaatcatgc aacagcttgg caaggattta aaaatggaag atggaacaga
     3361 cacgtagatg taagagagtt tatccaatta aactacactc tttatgaagg taatgattca
     3421 tttttagcag gaccaacaga agcaacttct aaactttggg aacaagtaat gcagttatcg
     3481 aaagaagaac gtgaacgtgg cggcatgtgg gatatggaca cgaaagtagc ttcaacaatc
     3541 acatctcatg atgctggtta tttagacaaa gatttagaaa caattgtagg tgtacaaact
     3601 gaaaagccat tcaaacgttc aatgcaacca ttcggtggta ttcgtatggc gaaagcagct
     3661 tgtgaagctt acggttacga attagacgaa gaaactgaaa aaatctttac agattatcgt
     3721 aaaacacata accaaggtgt attcgatgca tattctagag aaatgttgaa ctgccgtaaa
     3781 gcaggtgtaa tcactggttt acctgatgca tacgggcgtg gacgtattat cggtgactat
     3841 cgtcgtgtag ctttatatgg tgtagatttc ttaatggaag aaaaaatgca cgacttcaac
     3901 acgatgtcta cagaaatgtc agaagatgta attcgtttac gtgaagaatt atcagaacaa
     3961 tatcgtgcat taaaagaatt aaaagaactt ggacaaaaat atggtttcga tttaagccgt
     4021 ccagcagaaa acttcaaaga agcagttcaa tggttatact tagcatacct tgctgcaatt
     4081 aaagaacaaa acggtgcagc aatgagttta ggtcgtacat caacattctt agatatctat
     4141 gctgaacgtg accttaaagc aggtgttatt actgaaagcg aagttcaaga aattattgac
     4201 cacttcatca tgaaattacg tattgttaaa tttgctcgta cacctgatta caatgaatta
     4261 ttctctggag acccaacttg ggtaactgaa tctatcggtg gtgtaggtat tgacggacgt
     4321 ccacttgtta cgaaaaactc attccgtttc ttacactcat tagataactt aggtccagca
     4381 ccagaaccaa acttaacagt attatggtca gtacgtttac cagacaactt caaaacatac
     4441 tgtgcaaaaa tgagtattaa aacaagttct atccaatatg aaaatgatga cattatgcgt
     4501 gaaagctatg gcgatgacta tggtatcgca tgttgtgtat cagcgatgac aattggtaaa
     4561 caaatgcaat tcttcggtgc acgtgcgaac ttagctaaaa cattacttta cgctatcaat
     4621 ggtggtaaag atgaaaaatc tggtgcacaa gttggtccaa acttcgaagg tattaacagc
     4681 gaagtattag aatatgacga agtattcaag aaatttgatc aaatgatgga ttggctagca
     4741 ggtgtttaca ttaactcatt aaatgttatt cactacatgc acgataaata cagctatgaa
     4801 cgtattgaaa tggcattaca tgatacagaa attgtacgta caatggcaac aggtatcgct
     4861 ggtttatcag tagcagctga ctcattatct gcaattaaat atgcacaagt taaaccaatt
     4921 cgtaacgaag aaggtcttgt agtagacttt gaaatcgaag gcgacttccc taaatacggt
     4981 aacaatgacg accgtgtaga tgatatcgca gttgatttag tagaacgctt tatgactaaa
     5041 ttacgtagtc ataaaacata tcgtgattca gaacatacaa tgagtgtatt aacaattact
     5101 tcaaacgttg tatacggtaa gaaaactggt aacacaccag acggacgtaa agctggcgaa
     5161 ccatttgcac caggtgcaaa cccaatgcat ggccgtgacc aaaaaggtgc attatcttca
     5221 ttaagttctg tagctaagat tccttacgat tgctgtaaag atggtatttc aaatacattc
     5281 agtatcgtac caaaatcatt aggtaaagaa ccagaagatc aaaaccgtaa cttaactagt
     5341 atgttagatg gttacgcaat gcaatgtggt caccacttaa atattaacgt atttaaccgt
     5401 gaaacattaa tagatgcaat ggaacatcca gaagaatatc cacagttaac aatccgtgta
     5461 tctggttacg ctgttaactt cattaaatta acacgtgaac aacaattaga tgtaatttct
     5521 cgtacattcc atgaaagtat gtaacaaaat ttaaggtggg agcactatgc ttaagggaca
     5581 cttacattct gtcgaaagtt taggtactgt cgatggaccg ggattaagat atatattatt
     5641 cacacaagga tgcttactta gatgcttgta ttgccacaat ccagatactt ggaaaattag
     5701 tgagccatca agagaagtca cagttgatga aatggtgaat gaaatattac catacaaacc
     5761 atactttgat gcatcgggtg gcggtgtaac agtcagtggt ggcgaaccat tgttacaaat
     5821 gccattctta gaaaaattat ttgcagaatt aaaagaaaat ggtgtgcaca cttgcttaga
     5881 cacatcggct ggatgtgcta atgatacaaa agcatttcaa aggcattttg aagaattaca
     5941 aaaacataca gacttgatat tattagatat aaaacatatt gataatgaca aacatattag
     6001 attgacagga aagcctaata cacacatcct taacttcgcg cgcaaactgt cagatatgaa
     6061 acaacctgta tggattcgcc atgtccttgt gcctggttat tctgatgata aagacgattt
     6121 aattaaacta ggggaattta ttaattctct tgataacgtc gaaaagtttg aaattctgcc
     6181 atatcatcag ttaggtgttc ataagtggaa aacattgggc attgcatatg aattagaaga
     6241 tgtcgaagcg cccgatgatg aagctgttaa agcagcctac cgttatgtta acttcaaagg
     6301 gaaaattccc gttgaattat aaatacaatt cagaccgaaa agaaagcata tgcaacttca
     6361 agagtgaagg ggcatatgct tctttttcaa ttgagtattg attattaaca agacgtagta
     6421 aatatatgag acaagtccaa caatggttga aggaagacgc atttgtaagt agctatgctg
     6481 ataaagaatg tgatgccttg ttaaagatgg ggttccaata tcatcgaatg ggttattatt
     6541 tgctacttgc atacgaatat gagtcttttc aaatttttat tgaccctgag taatgaaaaa
     6601 tattaagatg aaacttaata ttaaagcaat gcggagcgtg attatgaaga gaattagtaa
     6661 agatatatgg gcagtattta aattactgta tcaaaataaa gggcgtttta gcattaatgc
     6721 cttactattg cagttaatca tgatttttat tagtagtaca tacttaattt tactatttaa
     6781 tatgatgtta aaagtagctg ggcaaagcca acttacgatt aacaattgga tggaaatcgt
     6841 aagtcaccct gccagtgtga tacttcttat tatattcata ttaagtgttg cctttctgat
     6901 ttatgtagag ttttcattgt tagtttatat ggtttatgcc ggctttgatc gacagattat
     6961 tacatttaaa tccattttta aaaatgcctt tgtcaatgtg cgtaaactca taggtgtacc
     7021 agttattttc tttgtcattt atttaatgtt aatgataccc attgccaacc taggactaag
     7081 ttcagtatta acaaaaaata tttacatacc taaattttta acggaagaac ttatgaaaac
     7141 gacgaaaggt ataatcattt acggtacctt tatgattgct gtatttatat taaattttaa
     7201 attaatattt accttaccgt taacgatttt aaaccgccaa tcgttattta aaaatatgag
     7261 actaagttgg caaattacga agcgaaataa gtttcgactt gttatagaaa tagttatatt
     7321 ggaactcatc attggtgcga ttttaacatt aattatttca ggagcaacat atcttgctat
     7381 ttgtgtagat gaagaaggag ataggttttt agtctcatca attttatttg ttgtattgaa
     7441 aagctcattg ttcttctatt atttatttac gaaattatca ttaatcagtg tgttagtact
     7501 gcacttaaaa caagagaatg tattagacca gccgggctta gaatttaaat acccaaaacc
     7561 gaaacggaag tctaggttct ttataatttc aatggtgctt gcagtgacgt gttttatcgg
     7621 ttataacatg tacttacttt acaataatac tatcaataca aatatctcca ttattggcca
     7681 tcgtggtttc gaagataaag gcgttgaaaa ttctattccg tcattgaaag ctgctgcaaa
     7741 agcgaatgtc gaatacgttg agttagatac aattatgacg aaagataaac aatttgttgt
     7801 tagtcatgat aacaatttga aacgtttaac aggtgttaat aaaaatattt ctgaatctaa
     7861 tttcaaagat gtcgtcggtt tgaaaatgcg tcaaaatgga catgaagcaa aacttgtatc
     7921 cttagacgaa tttattgaaa cggctaaaca atcaaatgtg aaactactag ttgagttaaa
     7981 gccacatggt aaagaaccag cagattatac acaacgtgtt attgatattt tgaaaaagca
     8041 tggtgttgaa catcaatatc gtgtgatgtc attggattat gatgtgatga ctaagttgaa
     8101 aaaagaagcg ccatatctca agtgtggtta tatcattccg ttgcagtttg gtcattttaa
     8161 agaaacatct ttagatttct ttgtcatcga agatttttct tattcgccaa gacttgttaa
     8221 tcaagcacac tcggaaaata aagaagtcta tacttggacc attaacggcg aagaagattt
     8281 aacgaaatac ttacaaacca atgttgatgg tattatcaca gatgacccag cattagctga
     8341 tcagattaaa gaagaaaaga aagacgaaac atacttcgat cgttctataa gaatattatt
     8401 tgagtaatat aaacaaagac ctctaaagtt atcaagatga tatcttcaga ggtcttttta
     8461 acgttgccat ctatggtata ggcaatcgtt tcattcgttt ataatcatat gacaaggatt
     8521 tataaggaaa tttggcgtca caaacactta catgatttat tggtgaatta ttaattgttt
     8581 tgtgaatgca aagggttaga aattgaatcg taaatacttt ctaatctatg tttcgcttta
     8641 gtcatttgat ccaaattttt agtgcgtata gctgatttag caatatagtg tgcagctaaa
     8701 atatcgcgtt tttgatacgc atctaaattt aggtgcgata atttatttaa gtcagtgttt
     8761 gctattaatt catgtaattg atctacaagc gcttgatgtt gatacgtatg tgatgtagtt
     8821 tcagatttgc ttgctaattt aataccagtc gtatcaagga gcgccgcttt aataccagca
     8881 actaaatatg ttttgatttt catttgtgtt gtcatgcttt gttactcctt tgatgtacat
     8941 taatcaaaaa aattatacac tattgtatat tgcaaagcta attaactata acaaaaagat
     9001 agttaatgct ttgtttattc tagttaatat atagttaatg tcttttaata ttttgtttct
     9061 ttaatgtaga ttgggcaatt acattttgga ggaattaaaa aattatgaaa aagcaaataa
     9121 tttcgctagg cgcattagca gttgcatcta gcttatttac atgggataac aaagcagatg
     9181 cgatagtaac taaagattat agtaaagaat caagagtgaa tgagaacagt aaatacgata
     9241 caccaattcc agattggtat ctaggtagta ttttaaacag attaggggat caaatatact
     9301 acgctaagga attaactaat aaatacgaat atggtgagaa agagtataag caagcgatag
     9361 ataaattgat gactagagtt ttgggagaag atcattatct attagaaaaa aagaaagcac
     9421 aatatgaagc atacaaaaaa tggtttgaaa aacataaaag tgaaaatcca cattctagtt
     9481 taaaaaagat taaatttgac gattttgatt tatatagatt aacgaagaaa gaatacaatg
     9541 agttacatca atcattaaaa gaagctgttg atgagtttaa tagtgaagtg aaaaatattc
     9601 aatctaaaca aaaggattta ttaccttatg atgaagcaac tgaaaatcga gtaacaaatg
     9661 gaatatatga ttttgtttgc gagattgaca cattatacgc agcatatttt aatcatagcc
     9721 aatatggtca taatgctaaa gaattaagag caaagctaga tataattctt ggtgatgcta
     9781 aagatcctgt tagaattacg aatgaaagaa taagaaaaga aatgatggat gatttaaatt
     9841 ctattattga tgatttcttt atggatacaa acatgaatag accattaaac ataactaaat
     9901 ttaatccgaa tattcatgac tatactaata agcctgaaaa tagagataac ttcgataaat
     9961 tagtcaaaga aacaagagaa gcaatcgcaa acgctgacga atcttggaaa acaagaaccg
    10021 tcaaaaatta cggtgaatct gaaacaaaat ctcctgttgt aaaagaagag aagaaagttg
    10081 aagaacctca attacctaaa gttggaaacc agcaagagga taaaattaca gttggtacaa
    10141 ctgaagaagc accattacca attgcgcaac cactagttaa aattccacag ggcacaattc
    10201 aaggtgaaat tgtaaaaggt ccggaatatc taacgatgga aaataaaacg ttacaaggtg
    10261 aaatcgttca aggtccagat ttcccaacaa tggaacaaaa cagaccatct ttaagcgata
    10321 attatactca accgacgaca ccgaacccta ttttaaaagg tattgaagga aactcaacta
    10381 aacttgaaat aaaaccacaa ggtactgaat caacgttaaa aggtactcaa ggagaatcaa
    10441 gtgatattga agttaaacct caagcaactg aaacaacaga agcatcacat tatccagcga
    10501 gacctcaatt taacaaaaca cctaagtatg tgaaatatag agatgctggt acaggtatcc
    10561 gtgaatacaa cgatggaaca tttggatatg aagcgagacc aagattcaac aagccaagcg
    10621 aaacaaatgc atacaacgta acgacaaatc aagatggcac agtatcatat ggcgctcgcc
    10681 cgacacaa
//
LOCUS       DAMZPP010000107        10414 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMZPP010000107 DAMZPP010000000
VERSION     DAMZPP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10414
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.107"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(280..684)
                     /locus_tag="POH73_002037"
     CDS             complement(280..684)
                     /locus_tag="POH73_002037"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDI6887970.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            complement(704..2116)
                     /gene="atpD"
                     /locus_tag="POH73_002038"
     CDS             complement(704..2116)
                     /gene="atpD"
                     /locus_tag="POH73_002038"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDI6887971.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            complement(2138..3004)
                     /locus_tag="POH73_002039"
     CDS             complement(2138..3004)
                     /locus_tag="POH73_002039"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDI6887972.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            complement(3035..4543)
                     /locus_tag="POH73_002040"
     CDS             complement(3035..4543)
                     /locus_tag="POH73_002040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDI6887973.1"
                     /translation="MAIKAEEISALLRSQIESYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            complement(4565..5104)
                     /locus_tag="POH73_002041"
     CDS             complement(4565..5104)
                     /locus_tag="POH73_002041"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDI6887974.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLK
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELVKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            complement(5104..5625)
                     /locus_tag="POH73_002042"
     CDS             complement(5104..5625)
                     /locus_tag="POH73_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDI6887975.1"
                     /translation="MTETANLFFLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            complement(5824..6036)
                     /gene="atpE"
                     /locus_tag="POH73_002043"
     CDS             complement(5824..6036)
                     /gene="atpE"
                     /locus_tag="POH73_002043"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDI6887976.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            complement(6079..6807)
                     /gene="atpB"
                     /locus_tag="POH73_002044"
     CDS             complement(6079..6807)
                     /gene="atpB"
                     /locus_tag="POH73_002044"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDI6887977.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            complement(6828..7280)
                     /locus_tag="POH73_002045"
     CDS             complement(6828..7280)
                     /locus_tag="POH73_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDI6887978.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQY
                     IQYYLYLLIILGGLYLFTHHAFILGLIIGVTGSAINTVIFESYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYFNKSHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            complement(7344..8474)
                     /gene="wecB"
                     /locus_tag="POH73_002046"
     CDS             complement(7344..8474)
                     /gene="wecB"
                     /locus_tag="POH73_002046"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDI6887979.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLNE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENLGQPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVRDVAHKILGGHDRIELIEPLDVIDFHNFAKQSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVDAGTLKVVGTHEQDVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDFN"
     gene            complement(8498..9127)
                     /gene="upp"
                     /locus_tag="POH73_002047"
     CDS             complement(8498..9127)
                     /gene="upp"
                     /locus_tag="POH73_002047"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDI6887980.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            complement(9155..10393)
                     /locus_tag="POH73_002048"
     CDS             complement(9155..10393)
                     /locus_tag="POH73_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDI6887981.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALEMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
BASE COUNT         3060 a         2121 c         1593 g         3640 t
ORIGIN      
        1 ggggccccaa caaagagaaa ttgtattccc aatttctaga gacaatgtaa gttggggtgg
       61 gacgacgaaa taaattttgc gaaaatatca tttctgtccc actcccattc ctatttttaa
      121 atacacatta gctgtgttta atgataaaga accactacat cataaatctt taatggttct
      181 ttatcaattc cttccccact ctcatgtttt gtatctttaa attaatataa ttcaaaaaag
      241 ggtcgaagat atgaaatcac atcttcgacc ctttatttac tattttaatt cagccacacg
      301 caaacggtta tttgctctct ctaaagctct ttcggctcta tgaatatcag tattgtcgtc
      361 atcattttcc aagtgagact ctgctcttgc tttagctaat ttagctcttt caacatcaat
      421 ttctcttgca gtttctgcag tctgaacaat gattgaaact ttatcttttc taacttcaac
      481 aaagccatcg cttacagcaa tatattcagt tccatcgtga aatttcactt ttacaaagcc
      541 tgtttttaaa gcagctacag ttggaatatg tccactcatg acacctatct caccagctgt
      601 tgtttgcata acaacgagtt caacattatc acgattgtaa acagaaccat taggagtgac
      661 aatatctagg tttaatgtat tcattatcca ttcctcctaa ttgttatact tcaacaccca
      721 tatctttagc ttttgcaata acatcatcca tgctaccaac taaacggaat gcatcttctg
      781 gaatatggtc atatttacca tctaagatat ctttaaagtt tgcaactgtt gtcttaacag
      841 gtacataaga acctttttga ccagtaaatt gttccgctac gtggaagttt tgagataaga
      901 agaattgaat tctacgtgcg cgttcaactg tttgtttatc ttcatcagat aattcgtcca
      961 tacctaagat agcaatgata tcttgtaatt cacggtattt ttgaagtgtt gattgtacat
     1021 cacgagctac ttcataatgt tcttgaccta caattgatgg ttccaatgct cttgatgtag
     1081 acgctaatgg atccacggct ggataaatac ccatttcagt taatttacgt tctaagtttg
     1141 tagttgcatc taaatgggca aacgctgtcg ctggcgctgg gtcagtatag tcatcggcag
     1201 gtacgaatac cgcttgaata gaagttactg atccttttgt tgtagacgta atacgttctt
     1261 gtaattgtcc catttcagta gcaagtgttg gttggtaacc tactgcagaa ggcatacgac
     1321 ctaataatgc agatacctca gaaccagctt gtgtaaatct gaaaatgtta tcgatgaata
     1381 ataatacgtc ttgaccttgt tcgtcacgga aatattcagc cattgttaaa ccagataatg
     1441 caacacgcat acgtgcacca ggtggctcat tcatttgccc gaataccatg gctgttttct
     1501 taattacacc actatcactc atttcgaagt ataaatcgtt accttcacga gtacgttcac
     1561 ctacaccggc gaatacagaa ataccaccgt gctcttgagc gatgttgtta attaattctt
     1621 ggattaatac tgttttacct acaccggcac caccgaacaa tccgatttta ccacctttaa
     1681 tataaggtgc tagtaaatct actactttaa tacctgtttc taaaatttga acttctgttg
     1741 aaagttcatc gaatgctggt gcttgacgat ggataggatc gcggcgaaca gaatcactaa
     1801 tttcttcttt aaggtcaatt gtttcaccta gtacattaaa tacacgacct aatgtttcat
     1861 caccaacagg tacactaatt tctttgcctg tatcttttac atccatgcct ctttggacac
     1921 catcagttga atccatcgca attgtacgaa caacgtcgtc acctaattgc agcgcaactt
     1981 ctaatgttag ttgtattgta ccttcttctt taggcacatc aataaccaag gcgttattaa
     2041 ttttaggaac ttcgttatgt tcaaatcgaa catcaattac aggacccata acttgagtta
     2101 cacggccaat tcccatgcta ttttcctcct ttaaatatta ttcaagcgct gcggaaccac
     2161 caacaatttc agtaatttgt tgcgtaattt ctgcttgtct cgctctgtta tattctaatg
     2221 ataagtcatc aataagttca gttgcattat cagtggcatt tttcatcgca gtcatacgtg
     2281 ttgcatgctc acttgctttt gcgtctaata ttgttccgta aatcaaactc tcaacatatt
     2341 gaggcaagat tacacttaag atagattctt tatctggctc aaattcataa gaagacaaat
     2401 gaccatgtcc cttactagaa tcctcttgag ataatggtaa tacttgtcta gatgtaggct
     2461 tgttttcaag aacgctgaca taatgactat agtatatatt taattcatca atttcttctt
     2521 cactgtataa gtctatagca tggttagcta gtgcttgaac agatttgaaa gaaggttgat
     2581 caggtacgtc tacttgagaa tactcaatgt cataacctct attttttagg aaatcaacac
     2641 cttgttgccc taaaactaca atactgtatt cgctactatc ttgatgtttc gcttcaatat
     2701 cagtaatcaa ttttttaagc acgtttgcac tatatgcacc tgctaaacct ttatcactcg
     2761 tgataactaa atatccactt ctagtaattt tacgaggtct tagcattgga tggtttgtat
     2821 tgctacttgc ccctgcaact gcagtaattg catcttgcat tttatccata tatggtgtga
     2881 attgttttgt atttttttca gctctacgaa gttttgaact tgataccatg ttcatcgctt
     2941 tcgtaatctg cttcattttt ttggttgatt ttattcgagt atctatttct ttaagagaag
     3001 ccactatctc accaccttat actaaacttg tttattattc agatttgcta aagctttttt
     3061 tgaattcgtt aattgctgtg tcaaacttct cagcatctgg taagccacca gtttctctga
     3121 tttcatttaa cagttcagta gcatttgatt ctgcccagtg gtttaactcg tcttcaaaac
     3181 gtgtgatatc tacaactgga atatcatcta aatatccttt tgttaatgca taaataatca
     3241 acacttggtg ttcaactggt aatggtttgt tttgatcttg ttttaagact tcaacagtac
     3301 gtttaccacg ttctaattta cttgcagtaa attcatcaag gtctgaaccg aattgtgcaa
     3361 atgattcaag ttctctatat gacgctaagt caagacgtaa cgtaccagca actttcttca
     3421 ttgctttaat ttgtgcagat ccaccaacac gagatacaga ttgtccggca ttaatcgctg
     3481 gtcttacacc tgagaagaat aaatcagatt gtaagaagat ttgtccatct gtaattgaaa
     3541 taacgtttgt tggtacataa gctgaaatat caccagcttg tgtttcgata attggtaatg
     3601 cagtaattga accgccacct aagtcatcgt ttaattttgc tgctctttct aataatctac
     3661 tatgtaagta gaatacgtca cctgggtatg cttcacgacc tggaggtcta cgtaataata
     3721 atgataactc acgataagct gctgcttgtt tagttaaatc atcatatacg attaaaacat
     3781 gtttaccgtt aaacatgaat tcttcaccca ttgttacacc tgaatatggt gcaatatata
     3841 ataatggaga aggttcagaa gctgatgctg ctacaacaat agtgtagtct aaagcgcctg
     3901 cttgtcttaa cttttcaaca tttgctctta ctgttgaatc tttttgacca atagcaacat
     3961 agatacaaat cgtaccttga tctttttggt tcaaaattgt gtcaattgca attgttgttt
     4021 tacctgtttg acggtcaccg atgattaact cacgttgacc tctaccaatt ggtactaaag
     4081 catcaattgc tttgatacct gtttgtaatg gctcatctac tgatttacga tccattacac
     4141 cagtagcttt tttctctact ggacgtgttt tagttgtgtt aatcggtcct tgtccatcaa
     4201 taggttgtcc taatggatta acaactcttc cgattagttc ttcacctact ggtacttcca
     4261 tgatacgacc agtacgttta acttcgtcac cttcagtaat acctgtgtat ggtcctaaaa
     4321 taaccacacc cacgtttgac tcttcaaggt tttgggctaa accaagtacg ccgttatgga
     4381 attctactag ctcaccagcc ataacgtcat ttaatccgtg aattaatgca ataccatcac
     4441 caatttgtaa tacagtacct acatcagtta cggacatttc tgactcataa ctttcaattt
     4501 gtgagcgaag taatgcactg atttcttcag cttttatggc catctatgtc actcctcttt
     4561 ataattaatt aactcttcta aattttcttt gtaattggac aagatcattt ctaacactac
     4621 catctaatac agttgtgccg actttaactc taaatccacc aattaaatct ggattaattt
     4681 ttgtatctac aataacttta gataacttcg tttgttgagt tactagtttg acaatcttaa
     4741 ctaactcttc ttgactcaat tcatatgttg actcaattgt tgcaaaatct tgattgtagt
     4801 gtccgttata taagctttgg aacgccttga atacgtcagc tattaatgag atatgtctat
     4861 tatctgctaa cacgtacatc atatttttaa tgtatggatt aatgtcagta aatacaccat
     4921 taatcaactc acgacgttgt tctgctgttt gagttggatt actgtcaacc attttcaatt
     4981 gctcaatttt atcttttact gcttcgttta taactgttaa ttcttcatta atagtttcta
     5041 aattatttgt atctaatgac acgtcaaata atgctttagc atacttgtta gctactttta
     5101 ccattattta tcgcctgcct cttttagata cttgtcaacc aatgcttttt ggtcttgttc
     5161 agaaatttct tttctaagaa ctttagaagc aattaacact gatagttcag atacttgatt
     5221 attaatatct gcaatggcac gttctttttg gctattgatt tcactttgtg ctgtttcaat
     5281 cataccgttt gcacgtacgt ttgcttcatg aataatttgt tcttgctgtt gacgtgcttg
     5341 aaccttagca tcttctaaaa tcttttgaac ttcttcttgt gtttctttaa gtttttgttt
     5401 attttcttct tcaagtttct gtgcatttaa cttagcttgt tctgcgtcat cgatatctct
     5461 gttaatatct ctttcacgtt tatccattac atctttcaat ggaccccatg cgaacttttt
     5521 aagtaacgca agtaatacga tgaaagttag gacctgtaca atcacagtac cccactcaac
     5581 gcctccagct gcaccaagaa agaataagtt agctgtttca gtcacgggtt catgacactc
     5641 ctttcattaa aataatcgct agtgttatat agaaaaaaac gacgaggtta ggacctactg
     5701 aatttatgcg ataggcatgg tgcgttcata tctcaactat ttaataaagt tcaaagatat
     5761 tactatatat ggcatcatga attatgtcct atgcgctttg tcccgacctc ttttatctgt
     5821 taattatcca gcaaatgtca tgaatgcaat tactacaccg atgataggta atgcctcaac
     5881 taaacctaca ccaatgaaca tgatacccat taattgacca cgtgcttctg gttgacgtgc
     5941 tacaccttca actgttcttg aaacgattaa accgttaccg atacctgctc ctaatgctga
     6001 taaaccaatt gcgattgctg ctgcgattaa attcataatt taatttcctc ctgtgattgt
     6061 atataattat tgaaattttt agtgttcatc tgccacttta tgtgacatat aaaccatcga
     6121 aagcataata aagatatatg cttggattgt tcctacaaat attgaaaatg cttgccaaac
     6181 aattaatcct gggatactaa taatccaacc ccatgctggt tcgttaaaga ataagccagc
     6241 aagtaatgtt aatagtatct cacctgcaaa tatgttaccg tacaaacgca gaccaagcgt
     6301 taatgttgaa gtgaactctt caaaaacatt aataattgcc aatggccaaa acggctgtac
     6361 ataaccttta agatattgtt tcgtaccacg cattttaatt ccataaaagt gagttaacag
     6421 tattatcgtt gtagacaacg ttaaagtcac tgttgcatca gctgttggtg atttccacca
     6481 caatgtgtga tctttcgtta ctattgaaaa cggaagacct aacatattag ctataaaaat
     6541 gtacagaatc agcgttactg ctaagaagtg gaattgacca ccttttttcc aagccatgtt
     6601 accttcaatg attcccctca cgaaatcaaa aatccattca acgaaatttt gtttgccagt
     6661 tggtcttttt ttcaaattac gcgtacaaat gatagcaagt agaaaaacaa gaaacgccgt
     6721 aacaagtatc atcaatatac ttgataaatt gaaaacgata tcaaaaccga ataaattcca
     6781 actcacgagc ggggatttgt gatccataat ctcacctctt tcttaattta tttgctcacc
     6841 tttagtaaag gacgtatgat aactacaaca tatgaaatca ttaaaccaat aattataccg
     6901 attatactta catgcgattt attaaagtac caaatcatac aggcaataat tgcaactaag
     6961 tatcgccaca tatttccagt tgaaatatgc atagtgtctg gtcttttagc tttggctaag
     7021 tagctctcaa atataacagt attgattgca gaaccagtaa caccaatgat taatcctaag
     7081 ataaatgcat ggtgtgtaaa aaggtataat cctcccaata ttattaataa atacaaataa
     7141 tattgaatgt actgtttaaa aatgttgtag aaacgactca ttttatatgc ctccgtgtga
     7201 ctgatatcaa tcatgaaaac cgttccactc atacaaacta aatgataata taggcgattt
     7261 gtggtgtcaa ttctattcaa attgttgtaa aaaaataagc atgttcagac acaatttaga
     7321 cacatttaga caaaaagtct tttttaattg aaatcgcttg gtttttcagt gattaaattc
     7381 aaataatact taatatgatt aactattctt tctgaagcaa atccatcacc gtaaggatta
     7441 gatgcttcag acatttgatg atataatctc tcatcatcga ttaattcttt tgcagcttga
     7501 tatacgtctt gttcatgcgt accaacaact ttcaacgtac cagcatcaac accttcaggt
     7561 cgttctgtaa cgcttcgcaa tactaggaca ggcttattaa aagatggcgc ctcttcctgt
     7621 atgcctcccg aatccgtcaa aataaaatag gattgtttag caaaattatg gaagtctatt
     7681 acatctaagg gttcaatcaa ttcaattctg tcatgccccc ctaaaatctt atgagccaca
     7741 tctcggactt ttggattttt atgcattgga tatactaacg ctaaatcggt gtactcatct
     7801 atcaagcgtc taactgcttt aaatatattt tccataggtt gacctaaatt ttcacgacgg
     7861 tgtgctgtca taagaatgaa ctttttgtca tggtatttat ccatgatgtt agatttataa
     7921 ttgtcatcaa ctgtatattt catagcatca atcgctgtat taccagtgac aacaacactt
     7981 tctgaatatt tcccttcatt taacaaatgc gatgcagcat ttttagtagg tgcaaaatgt
     8041 aagtcagcta atacaccaac taattgtcta ttcacctctt ctggaaaagg tgaatattta
     8101 tcataacttc taagccctgc ttcaacgtgt ccaatcggca cttggttata aaatgccgct
     8161 aaaccacctg caaatgtcgt catcgtatca ccatgtacaa gtaccatgtc tggtttttct
     8221 aattgaatca cttgttctaa ttgagtgatt gatttagaag ttatctcaga aagtgtctgt
     8281 cctgatttca taatattcaa atcgtatttt ggtttgattt caaaggtact taatactgaa
     8341 tcaagcattt ctctatgctg tgctgtaaca acaacaattg gctcgagcat tttttcttgt
     8401 tccaaagctt taactaaagg agccatcttt atggcttcgg gtcttgttcc aaatatggtc
     8461 ataatctttt tcatcaaact acttatctcc gattcttcta tttagtacca aacaatctat
     8521 ctccagcgtc acctaaccct ggtgtgatat atgctttgtc atttagcttt tcatcaagtg
     8581 cagcaatata aatatctaca tctggatgtg cttcatgcat cttttctacg ccttctggtg
     8641 ctgcaattaa acacatgaag cgaatatttt tagcgccacg tttcttcaat gaagtaatag
     8701 cttcaattgc tgatgcgcct gttgctaaca taggatcaac aacaatgatt tgtctttcag
     8761 taatatcttg aggtaactta gcaaaatact ctacagcctt taatgtttcg ggatctcgat
     8821 acaaaccgat atgtccaact ctcgctgcag gtactaaact taaaatacca tcagtcatac
     8881 ctaaaccagc tcttaaaatt ggaacgatag ctaatttttt accagctaat cgtttagccg
     8941 tcattttagt tacaggcgtt tcaatatcaa catcctgaag ctctaagtct ctagttactt
     9001 catatgccat caacatacca acttcgtcta caagttctct aaattcttta gtacctgtat
     9061 ttacgtctct aatatagctt agtttgtgtt gaattaatgg atgatcgaaa acgtgtactt
     9121 tactcataaa aattactcct atctttgtgt atgtttattg atatagagga tattcagctg
     9181 ttaatttcgc aacgcgttct ttagcttgtt gtaatttttc ttcatcttta ctatttttca
     9241 atgctaaact gatgattttt gcaacttcct caaaagcctt ttcatcaaat ccacgcgttg
     9301 ttgcagcagg tgtacctaaa cgtataccac tcgttacaaa aggtttttct tgatcgaacg
     9361 gaatggtatt tttgttacat gtgataccaa ctgaatctaa agtctcttca gcttctttac
     9421 cagtaagtcc tatagaccct tttacatcaa cagctactaa gtgattatct gtaccaccag
     9481 aaacaattct aaatccttca ttaattaatg cttctgcaag aacttttgcg tttttaacca
     9541 cttgttgttg atacgttttg aaattatttt ctaacgcttc tccaaaagca actgcttttg
     9601 ctgcaataac atgctcaaga ggtccacctt gaataccagg gaaaattgtt ttatctatgt
     9661 cttttttata ttcttcctta cataaaatca taccaccacg tggtccgcgt aatgttttgt
     9721 gtgttgtagt tgttacaaaa tcagcatatt ctactggatt tggatgtaaa cctgccgcta
     9781 ctaatcctgc aatatgtgcc atgtctacca ttaacttagc gtttacttca tctgcgattt
     9841 ctttaaactt tttgaaatca attgttcttg aatatgctga tgctcctgcc acaataagct
     9901 taggcttatg ctctaacgct aatttacgaa cttcatcata attgattcgt tctgtgtctt
     9961 tatctactcc atattcaacg aaattgtaga atttaccact aaaattaaca ggcgctccat
    10021 gtgtcaagtg accaccatga ctcaaattca tacctaaaac tgtgtcgccc atttctaatg
    10081 caactaagta aacagccatg ttcgcttgtg aacctgaatg tggttgaaca ttgacatgtt
    10141 cagctccaaa caacgcttta gcacgatcaa ttgcgatgct ttcagtaaca tctacaaact
    10201 cacagccacc ataatatcgg cgtcctggat agccttcagc atacttatta gtcaacactg
    10261 aaccttgtgc ttccataacc gcttccgata caaaattttc cgatgcgatt aactctatgt
    10321 tgctattttg tctctgaaat tctctctcga ttgcttctgc gataacttta tcttgcttgg
    10381 tgatataaga cataaaatct ccccttcttt caaa
//
LOCUS       DAMZPP010000108        10343 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMZPP010000108 DAMZPP010000000
VERSION     DAMZPP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.108"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..1068
                     /gene="sbnF"
                     /locus_tag="POH73_002049"
     CDS             <1..1068
                     /gene="sbnF"
                     /locus_tag="POH73_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HDI6887982.1"
                     /translation="QVDLAEERLNGTVLWLGESDELYHPQQSIRTMSPIDTTKYYLKV
                     PISITNTSTKRVLAPHTIENAAQITDWLKQIQQQDTYLKDELKTAFLGEVLGQSYLNT
                     QLSPYKQTQVYGALGVIWRENIYHMLIDEEDAIPFNALYASDKDGVPFIENWIKQYGS
                     EAWTKQFLAVAIRPMIHMLYYHGIAFESHAQNMMLIHENGWPTRIALKDFHDGVRFKR
                     EHLSEAASHLTLKPMPEAHKKVNSNSFIETDDERLVRDFLHDAFFFINIAEIILFIEK
                     QYGIDEKLQWKWVKGIIEAYQEAFPELNNYQHFDLFEPTIQVEKLTTRRLLSDSELRI
                     HHVTNPLGVGGITDATTISET"
     gene            1043..1819
                     /gene="sbnG"
                     /locus_tag="POH73_002050"
     CDS             1043..1819
                     /gene="sbnG"
                     /locus_tag="POH73_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HDI6887983.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDED"
     gene            1819..3021
                     /gene="sbnH"
                     /locus_tag="POH73_002051"
     CDS             1819..3021
                     /gene="sbnH"
                     /locus_tag="POH73_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HDI6887984.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERTILDTISQYVEGFEVASQGEIAKGLVFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPNIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHQF
                     PLKHINLGGGIGVNYADLTSQFEWDNFVENFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREIQIEAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            3025..3789
                     /gene="sbnI"
                     /locus_tag="POH73_002052"
     CDS             3025..3789
                     /gene="sbnI"
                     /locus_tag="POH73_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HDI6887985.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            3985..4611
                     /locus_tag="POH73_002053"
     CDS             3985..4611
                     /locus_tag="POH73_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6887986.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISNGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVSTSDTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEFKNPTTKNLEGLNEEVHKDEATTKVDSDNTEPPIESKDHVSKKD"
     gene            4822..5598
                     /locus_tag="POH73_002054"
     CDS             4822..5598
                     /locus_tag="POH73_002054"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HDI6887987.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            5742..5863
                     /locus_tag="POH73_002055"
                     /pseudo
     CDS             5742..5863
                     /locus_tag="POH73_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5933..6913
                     /locus_tag="POH73_002056"
     CDS             5933..6913
                     /locus_tag="POH73_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDI6887988.1"
                     /translation="MEDLEKVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LKLLETIKKYNSHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAVGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHDFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            6876..7565
                     /locus_tag="POH73_002057"
     CDS             6876..7565
                     /locus_tag="POH73_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDI6887989.1"
                     /translation="MKKLQRKKWKCREIGIEAVRNKGYAYQRKLDTLEEVKKGYFPIK
                     RAIDLVLSIVLLFLTFPIMFIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMCK
                     NAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVEL
                     FSSEIIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIRT
                     LMVVITGEGSR"
     gene            7775..8941
                     /locus_tag="POH73_002058"
     CDS             7775..8941
                     /locus_tag="POH73_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDI6887990.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHHDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVSPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRVVFTAHGWAFTEGVKPAKKFLYLVIEKLMSRITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINDSTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENDVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVTDNGICIV
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            8922..10160
                     /locus_tag="POH73_002059"
     CDS             8922..10160
                     /locus_tag="POH73_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDI6887991.1"
                     /translation="MENQHNSKYLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMLVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMIYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            10150..>10343
                     /locus_tag="POH73_002060"
     CDS             10150..>10343
                     /locus_tag="POH73_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDI6887992.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLY"
BASE COUNT         3501 a         1507 c         2026 g         3309 t
ORIGIN      
        1 caagttgatt tggctgagga aaggcttaat ggcacagtac tatggttagg ggaaagtgat
       61 gagctatatc accctcaaca atcgattcgt acgatgtcgc caatagacac gacaaaatat
      121 tatttaaagg taccaataag tataacgaat acttcaacga aacgagtgtt ggcacctcat
      181 acaattgaaa atgcagcgca aattacggat tggttaaagc agatacagca acaagatacg
      241 tatttaaaag atgaattaaa gacagctttt ctaggggaag tcttaggaca gtcttattta
      301 aatacacaac tttcgcctta taaacaaact caagtttatg gtgcgttagg tgttatatgg
      361 cgtgaaaata tatatcatat gttaatcgat gaagaggatg cgataccatt taatgcactt
      421 tatgcgagtg ataaggatgg tgtaccattc attgaaaatt ggattaaaca atatggttct
      481 gaagcttgga caaagcaatt tttagctgta gcgattcgtc caatgattca tatgctttat
      541 tatcacggta ttgcctttga atcgcatgca caaaatatga tgctcattca tgaaaatggt
      601 tggcctacac gtattgcctt aaaagatttc catgatggtg ttcgttttaa gcgtgagcat
      661 ttaagtgaag ctgcttcaca cctgacatta aagccaatgc cagaagcaca taaaaaagtg
      721 aatagtaatt catttattga aacagatgat gaacgtttag tacgtgactt tttacatgat
      781 gcatttttct ttattaatat cgccgaaatc atcttattta ttgaaaagca atatggtatc
      841 gatgagaagc tgcaatggaa atgggttaaa ggcatcattg aggcgtatca agaagcattt
      901 ccagagctga ataactatca acattttgat ttgtttgaac ctacgattca agttgaaaag
      961 ttaacgacac gtcgattatt aagtgactcc gagttaagaa ttcatcatgt tacaaatcca
     1021 ttaggtgtag gaggtattac tgatgcaaca actatctctg aaacatagat taaacaatgg
     1081 tgattcagtt tatggcattt ttaattctat accggaccca ttgatgatag aggttatcgc
     1141 agcaagcggg tatgactttg ttgtgattga tacagaacac gtggcgatta atgatgagac
     1201 actagcgcat ttaattcgtg cagctgaagc agcgcatatt ataccaattg tacgtgtcac
     1261 tgcagtgata gatagagata tcattaaagt gttggatatg ggtgcgagag gtattattgt
     1321 gccacacgtt aaagatcgtg agacagttga gcatattgtg aaattaagtc gttattaccc
     1381 gcaaggatta agaagtttga atggtggtcg catggcaaga tttggacgta caccattact
     1441 tgatgcaatg gagatggcta atgagcatat tatggtgatt gccatgatag aagatgttga
     1501 aggggttatg gccattgacg atatagcaca agtcgaaggt ttagacatga tagtcgaagg
     1561 tgccgcagat ttatcccagt cacttggcat accatggcaa acgcgtgatg atcaagtaac
     1621 atcacatgtt caacatattt ttgaagttgt gaatgcacat ggtaaacatt tttgtgcatt
     1681 accacgtgaa gatgaagata ttgcaaaatg gcaggcacaa ggtgtacaaa catttatttt
     1741 aggtgatgat cgcggaaaaa tatatcgcca tttaagtgca tctctagcga cgtctaaaca
     1801 gaagggggat gaagactaat gcgtatagtt caacctgtta ttgaacagtt aaaagcacaa
     1861 tctcatccag tttgtcatta tatctatgat ttagtcggac tggaacatca tttgcaacat
     1921 attacatcgt cattgcctag taattgtcag atgtactatg caatgaaagc aaatagtgaa
     1981 cgaacaatcc tagatacaat tagtcagtat gttgaaggat tcgaagttgc atctcaaggt
     2041 gaaatagcaa aaggtcttgt ttttaaacca gcaaatcata ttatttttgg tggccctggt
     2101 aagacagacg aggaactaag atatgcagta agtgaaggtg ttcagcgtat tcatgttgaa
     2161 agtatgcatg aattacaacg gctaaatgcc atcttagaag atgaagataa gacacaacac
     2221 attttattgc gtgttaattt agcaggacca tttcccaatg caacgttgca tatggcagga
     2281 cgcccaacac aatttggtat ttctgaagac gaagttgatg atgtcattga agctgcgcta
     2341 gcaatgccaa atattcatct agatggattt cattttcatt ctatttctaa caatttagac
     2401 tcgaatttac atgtcgatgt agtgaaactt tattttaaaa aagcaaaggc atggtctgaa
     2461 aaacatcaat ttcctctcaa acatatcaat cttggtggtg gcataggcgt taactatgca
     2521 gatttaacta gccaatttga gtgggataat tttgtagaaa attttaaaac acttatcgtt
     2581 gagcaagaaa tggaagatgt gacattgaac tttgaatgtg ggcgctttat tgtggcacat
     2641 attggttact atgtgacaga agtgctagac attaagaaag tacatggtgc ttggtatgcc
     2701 attttaagag gaggtacgca acaatttaga ctgccggtat cttggcagca taaccatcct
     2761 tttgacattt atcgctataa ggacaatcca tattcatttg aaaaagtttc aatttcgaga
     2821 caggacacaa cgttagtcgg tcaattatgt acaccgaaag atgtctttgc tagagaaata
     2881 cagatagagg caatcagtac aggcgacgtt attgttttca aatatgcagg tgcatacgga
     2941 tggtctattt cacatcacga tttcttaagc catccacatc ctgaatttat ttatttaacg
     3001 caaacaaagg aggatgaata actattgaat catattcatg aacatttaaa attggtacca
     3061 gtagataaga ttgatcttca cgaaacattc gaacctttaa gattggaaaa aacgaaaagt
     3121 agtattgaag cagatgattt tatacgtcat cctattttag tgacagcgat gcaacatggt
     3181 agatatatgg ttatagatgg tgtgcatcgg tatacaagtt tgaaagcgtt aggatgtaag
     3241 aaagttccag tgcaagaaat ccatgaaaca caatattcaa ttagtacatg gcaacataaa
     3301 gttccatttg gtgtgtggtg ggaaacgtta caacaagaac atcgcttacc atggactact
     3361 gagacaagac aagaagcgcc atttattacg atgtgtcatg gtgatacaga acaatatttg
     3421 tatacaaaag atttaggcga agcacatttt caagtatggg aaaaggttgt cgcaagttat
     3481 agtggttgtt gttctgtaga gagaattgca caaggtacat atccttgtct ttctcaacaa
     3541 gatgtactca tgaagtatca gccattgagt tataaggaaa ttgaagcggt tgttcataaa
     3601 ggggaaactg tgccagcagg tgtgacacgc tttaatattt caggacgatg tcttaatctt
     3661 caagtaccac tggcattact taaacaagat gatgatgttg aacaactgcg caattggaag
     3721 cagtttttag cagataagtt tgccaatatg agatgttata ctgaaaaagt atacttggtg
     3781 gagcaatagt tttactgtga tgttgaggga aatatgatga tttagcctat taatagcgaa
     3841 aatataataa aacaatatag tgtggagaac ttttgatatt ttataaatat tgaagttctc
     3901 catttttgta ttttgcatat aaaaattaaa taaaataagg tatattaagg taaagtataa
     3961 atattaaata aatggggaat agacatggaa aaaaatgtag aaaaatcatt cataaagata
     4021 ggtttatatt ttcaaatagc ttatatagta ctcatggcta taactttatg tgggtttgta
     4081 atttgctatg gactaatttt cggccttttc tatttattgt caggtagcag agctgattat
     4141 ttaatagtaa cgatagttat atcggcaata atttctatat ttgtaattat actttcaatc
     4201 gtacctgtca tcgtattggc atctgactta tttaaagaaa ggatttcaaa tggtgtcata
     4261 ttaattgtat tggctattat cgctttagta ttatgcaact ttgtatctgc aatactctgg
     4321 tttgtttcag ccatatctat tttaggtaga aaaaaattag tatctacatc agatactacc
     4381 actattcaaa aaagtaaagg gaacgcaaat caagcatcac ataaagacac gtgtaaaaag
     4441 gaacttgata gtcaagacat gatggaacat cctgagttta aaaatcccac gactaaaaac
     4501 ctagaaggat taaacgaaga agtacataaa gatgaagcta caactaaagt tgacagtgat
     4561 aatacggaac cgccgattga atcaaaagac catgtctcga aaaaagattg atgacaaact
     4621 aatcgagaga cttaaaaaat aatattcaac ataaacactt ttaaaacgac atttaaacgc
     4681 attgccaatc actaaaggta gtgcgtttaa ctatacctta aatatctgaa tattttgtta
     4741 aatggagtta cctttgttgt actattcaaa tgaagaggag taaaatgtaa ttaaaggaaa
     4801 gtaatttgag gagtgatctt tatgacaaac aacaaagtag cattagtaac tggcggagca
     4861 caagggattg gttttaaaat tgcagaacgt ttagtggaag atggtttcaa agtagcagtt
     4921 gttgatttca atgaagaagg ggcaaaagca gctgcactta aattatcaag tgatggtaca
     4981 aaagctattg ctatcaaagc agatgtatca aaccgtgatg atgtatttaa tgcagtaaga
     5041 caaactgcag cgcaatttgg cgatttccat gtcatggtta acaatgccgg ccttggacca
     5101 acaacaccaa tcgatacaat tactgaagaa cagtttaaaa cagtatatgg cgtgaacgtt
     5161 gcaggtgtgc tatggggtat tcaagccgca catgaacaat ttaaaaaatt caatcatggc
     5221 ggtaaaatta tcaatgcaac atctcaagca ggcgttgagg gtaacccagg cttgtcttta
     5281 tattgcagta caaaattcgc agtgcgaggt ttaacacaag tagccgcaca agatttagcg
     5341 tctgaaggta ttactgtgaa tgccttcgca cctggtatcg ttcaaacacc aatgatggaa
     5401 agtatcgcag tggcaacagc cgaagaagcg ggtaaacctg aagcatgggg ttgggaacaa
     5461 tttacaagtc agattgcttt gggcagagtt tcacaacccg aagatgtttc aaatgtagtg
     5521 agcttcttag ctggtaaaga ctctgattac attactggac aaacaattat tgtagatggt
     5581 ggtatgagat tccgttaata atcatccatt aataataaat aaatccttat tgttaagttt
     5641 aatcacttag cagtaaggat tttttagtgc acttagaagg gattgtatta gtagaaaatt
     5701 aataacagaa gctcttaagt gagttatgat gtcacagtct aatgcatcag ttgaaagcat
     5761 tactaaattc tatcaagcat agttacgcta aaacatcata aaacaccact atctaattta
     5821 tctacataaa aattcacaaa gttatcgcat tttattttta taaataaaaa ataccgataa
     5881 aaagcttaca atactttatg tttttgtgat atatttttaa tgtataaatg aggtggaaga
     5941 tttggaaaaa gttttgataa ctggtggggc tggttttatt gggtcacatt tagtagatga
     6001 tttgcaacaa gattatgatg tttatgttct agataactat agaacaggta aacgagaaaa
     6061 tattaaaagt ttggctgacg atcatgtgtt tgaattagat attcgtgaat atgatgcagt
     6121 tgaacaaatc atgaagacat atcaatttga ttatgttatt catttagcag cattagttag
     6181 tgtcgctgag tcggttgaga aacctatctt atctcaagaa ataaacgtcg tagcaacgtt
     6241 gaaattatta gaaaccatta aaaaatataa cagtcatata aaacgattca tctttgcatc
     6301 atctgcagcc gtttatggtg atttacctga tttacctaaa agtgatcaat cattaattct
     6361 accattatca ccatatgcaa tagataaata ttacggcgaa cgtacgacat taaattattg
     6421 ctcgctatat aacattccaa cagctgttgt taaattcttt aatgtatttg ggccaaggca
     6481 agatcctaag tctcaatatt caggtgtgat ttcaaaaatg ttcgattcat ttgaacataa
     6541 caagcctttt acattttttg gcgatggtct gcaaacgaga gactttgttt atgtttatga
     6601 tgttgttcaa tctgtgcgct taattatgga gcataaagat gcggttggac atggttataa
     6661 cattggtacg ggtacattta cgaatttgct agaggtttat cgcattattg gtgaattata
     6721 tggaaaatca gtcgagcatg attttaaaga agcaagaaag ggagatatta agcattctta
     6781 tgcagatatt tctaatttaa aagcattagg atttgttcct aaatacactg tggaaacagg
     6841 cttaaaggat tactttaatt ttgaggtaga taatattgaa gaagttacag cgaaagaagt
     6901 ggaaatgtcg tgaaattggc attgaagctg tccgtaataa gggttacgcc tatcaaagaa
     6961 aattagacac actagaagaa gtaaaaaagg gctattttcc aatcaaacgt gcgattgact
     7021 tggtattaag tatcgtttta ttatttttaa catttccgat tatgttcata ttcgccattg
     7081 ctatcgtcat agattcgcca ggaaacccta tttatagtca ggttagagtt gggaagatgg
     7141 gtaaattaat taaaatatac aaattacgtt cgatgtgtaa aaacgcagag aaaaacggtg
     7201 cgcaatgggc tgataaagat gatgatcgta taacaaatgt cgggaagttt attcgtaaaa
     7261 cacgcattga tgaattacca caactaatta atgttgttaa aggggaaatg agttttattg
     7321 gaccacgccc ggaacgtcca gaatttgtag aattatttag ttcagaaata ataggatttg
     7381 agcaaagatg tcttgttaca ccaggattaa caggtcttgc gcaaattcaa ggtggatatg
     7441 acttaacacc acaacaaaaa ttaaaatatg acatgaaata tatacataaa ggtagcttaa
     7501 tgatggaatt atatatatca attagaacat tgatggttgt tattacaggg gaaggctcaa
     7561 ggtagtctta atttgcttaa taagtttaaa taaaagttac attttaaaga ttgtgaccaa
     7621 ttgttacagt ataacgagga atcccttgag acagtatcaa atggcattaa gaaatatttg
     7681 ccatcattga tttgcatggc tataaatact attcatctga tgagatagcc atgttaagaa
     7741 attgaaagta tagcattaaa ggggtttgta acagttgaaa attatatatt gtattactaa
     7801 agcagataat ggtggtgcac aaacacatct cattcaactc gctaaccatt tttgcgtaca
     7861 ccatgatgtt tatgtcattg taggcaatca tggaccaatg attgaacaac tagatgcaag
     7921 agtcaatgta attattatcg aacatttagt aagtccaatt gactttaaac aagatatttt
     7981 agctgtcaaa gtgttagcac agttattctc gaaaattaaa cctgatgtta tccatctaca
     8041 ttcttccaaa gctggaacgg tcggacgaat tgcgaagttc atttcgaaat cgaaagacac
     8101 acgtgtagtt tttactgcgc atggatgggc ttttacagag ggtgttaaac cagctaaaaa
     8161 atttctatat ctagttattg aaaaattaat gtcacgtatt acagatagca ttatttgtgt
     8221 ttcagatttc gataaacagt tagcgttaaa atatcgattt aatcgattga aattaaccac
     8281 tatacataat ggaattgcag atgttcccgc tgttaagcaa acgctaaaaa gccaatcaca
     8341 taacaatatt ggcgaagttg ttggaatgtt gcctaataaa caagatttac agattaatga
     8401 ctcgacaaaa catcaatttg ttatgattgc aagatttgct tatccaaaat tgccacaaaa
     8461 tctaatcgcg gcaatagaga tattgaaatt acataacagt aatcatgcgc attttacatt
     8521 tataggcgat ggacctacat taaatgattg tcagcaacaa gttgtacaag ctgggttaga
     8581 aaatgatgtc acatttttgg gcaatgtcat taatgcgagt catttattat cacaatacga
     8641 tacgtttatt ttaataagta agcatgaagg tttgccaatt agcattatcg aagcgatggc
     8701 tacagggttg cctgtcatcg ctagtcatgt tggtggcatt tcggaattag taactgataa
     8761 tggtatatgt atagtgaaca atcaacctga aactattgcc aaagtcctgg aaaaatattt
     8821 aatagacagt gattacatca aaatgagtaa ccaatctaga aaacgttatt tagaatgttt
     8881 tacagaggag aaaatgatta aagaagtgga agacgtttat aatggaaaat caacacaata
     8941 gtaaatatct aacattgtta cttatcggtt tagcggtttt tattcagcaa tcttcggtta
     9001 ttgccggtgt gaatgtttct atagctgact ttatcacatt actaatatta gtttatttac
     9061 tgtttttcgc caaccattta ttaaaggcaa atcatttttt acagtttttc attattttgt
     9121 atacatatcg tatgattatt acgctttgtt tactattttt tgatgatttg atatttatta
     9181 cggttaagga agttcttgca tctacagtta aatatgcatt tgtagtcatt tatttctatt
     9241 tagggatgat catctttaag ttaggtaata gcaaaaaagt gatcgttacc tcttatatta
     9301 taagcagtgt gactataggt ctattttgta ttatagctgg tttgaacaag tcccctttac
     9361 taatgaaatt attatacttt gatgaaatac gttcaaaagg attaatgaat gaccctaact
     9421 atttcgcgat gacacagatt attacattgg tacttgctta caagtatatt cataattaca
     9481 tattcaaggt ccttgcatgt ggtattttgc tatggtcttt aactacaacg gggtctaaga
     9541 ctgcgtttat catattaatc gtcttagcca tttatttctt tattaaaaag ttatttagta
     9601 gaaatgcagt aagtgttgtg agtatgttag tgattatgtt aattctactt tgttttacct
     9661 tttataatat aaactactat ttattccaat taagcgacct tgatgcctta ccgtcattag
     9721 atcgaatggc gtctattttt gaagaggggt ttgcatcatt aaatgatagt ggatctgagc
     9781 gaagtgttgt atggataaat gccatttcag taattaaata tacattaggt tttggtgttg
     9841 gcttagtgga ttatgtacat attggctcgc aaattaatgg tattttactt gttgcccata
     9901 atacatattt gcaaattttt gcggaatggg gcattttatt cggtgcgtta tttatcatat
     9961 ttatgattta tttattattt gaattattta gattcaatat ttctgggaaa aatgtaacag
    10021 caattgttgt aatgttgacg atgctgattt actttttaac agtatcgttt aataactcaa
    10081 gatatgtcgc ttttatttta ggaattatcg tctttattgt tcaatatgaa aagatggaaa
    10141 gggatcgtaa tgaagagtga ttcactaaaa gaaaatatta tttatcaagg gctataccaa
    10201 ttgattagaa cgatgacacc gctgattaca atacccatta tttcacgtgc atttggtccc
    10261 agtggtgtgg gtattgtttc attttctttc aatatcgtgc aatacttttt gatgattgca
    10321 agtgttggcg ttcagttata ttt
//
LOCUS       DAMZPP010000109        10271 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMZPP010000109 DAMZPP010000000
VERSION     DAMZPP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.109"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..3423
                     /gene="sasC"
                     /locus_tag="POH73_002061"
     CDS             <1..3423
                     /gene="sasC"
                     /locus_tag="POH73_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HDI6887993.1"
                     /translation="RNAVNEKAREAITNINATPGATREEKQEAINRVNTLKNRALNDI
                     GVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLTDLATAKKQEINQNTNATTEE
                     KQVALNQVDQDLATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQTNQ
                     HYSAKLVEINATPDATDDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAENNI
                     DAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAFNQIN
                     QNQTNDQVDATTNQAINAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDNEK
                     EVALQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKIKPAAREKINQK
                     ANELRAQINQDKEATAEERQAALDKINDLVVKAMTNITNDRTNQQVNDSTNQALDDIA
                     LVTPDHIVRAAARDAVKQQYEAKKHEIEQAEHATDEEKQVALNQLANNEKRALQNINQ
                     AIANNDVKRVESNGIATLKGVEPHIVVKPEAQEAIKASADNQVESIKDTPHATTDELD
                     EANQQINDTLKQGQQDIDNTTQDAAVNDVRNQTIKAIEQIKPKVRRKRAALDNIDESN
                     NNQLDAIRNTLDTTQDERNVAIAALNKIVNAIKNDIAQNKTNAEVDQTEADGNNNIKV
                     ILPKVQVKPAARQSVSAKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQINHA
                     DKTAQVNQNSIDAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQNA
                     AIVQVEKELIKAKQQIAGAVTNADVAYLLHDGKNEIREIEPVINKKATAREQLTTLFN
                     DKKQAIEANVQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTATLNLQTIHDL
                     DVHPIKKPDAEKTINDDLARVTHLVQNYRKVSDRNKADALKAITALKLQMDEELKTAR
                     TNADVDAVLKRFNVALGDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKVKA
                     LIDQYVADGNRMVDEDATLNDIKKDTQLIIDEILAIKLPAEVIKASPKVGQPAPKVCT
                     PIKKEDKQEVRKVVKELPNTGSEEMDLPLKELALITGAALLARRRSKKEKES"
     gene            complement(3540..4808)
                     /locus_tag="POH73_002062"
     CDS             complement(3540..4808)
                     /locus_tag="POH73_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6887994.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQNDNVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIIDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIENQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKRISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDTFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAEIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
     gene            5212..6873
                     /locus_tag="POH73_002063"
     CDS             5212..6873
                     /locus_tag="POH73_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDI6887995.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNVHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVAIKLALNYPLIMLFHTPGAVLSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVEAVFFIANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            6870..7565
                     /locus_tag="POH73_002064"
     CDS             6870..7565
                     /locus_tag="POH73_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDI6887996.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKISVRGELIEYVENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEEDIQAFKEGITLSDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            7582..8004
                     /locus_tag="POH73_002065"
     CDS             7582..8004
                     /locus_tag="POH73_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDI6887997.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFEEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            8210..9619
                     /gene="sapep"
                     /locus_tag="POH73_002066"
     CDS             8210..9619
                     /gene="sapep"
                     /locus_tag="POH73_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HDI6887998.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLAEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAKAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            9623..>10271
                     /gene="dat"
                     /locus_tag="POH73_002067"
     CDS             9623..>10271
                     /gene="dat"
                     /locus_tag="POH73_002067"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HDI6887999.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAED"
BASE COUNT         3815 a         1543 c         1877 g         3036 t
ORIGIN      
        1 cgcaatgctg taaatgaaaa agcgcgagag gcgataacaa atatcaatgc tacacctggc
       61 gcgactcgag aagagaaaca agaagcgata aatcgtgtca atacacttaa aaatagagca
      121 ttaaatgata ttggtgtgac gtctactact gcgatggtca atagtattag agacgatgca
      181 gtcaatcaaa tcggtgcagt tcaaccgcat gtaacgaaga aacaaactgc tacaggtgta
      241 ttaacggact tagcaactgc aaaaaaacaa gaaattaatc aaaatacaaa tgcaaccact
      301 gaagaaaagc aagtagcatt aaatcaagta gaccaagatt tagcaacggc aattaataat
      361 ataaatcaag ctgatactaa tgcagaagta gatcaagcac aacaattagg tacaaaagca
      421 attaatgcga ttcagccaaa tattgtaaaa aaacctgcag cattagcaca aaccaatcag
      481 cattatagtg ctaaattagt tgaaatcaat gctacaccag atgcaacaga tgatgagaaa
      541 aatgctgcga tcaatacttt aaatcaagac agacaacaag ctattgaaag tattaaacaa
      601 gcaaatacaa atgcggaagt agaccaagct gcgacagtgg cagagaataa tatcgatgct
      661 gttcaagttg acgttgtaaa aaaacaagca gcgcgagata aaatcactgc tgaagtagcg
      721 aagcgtattg aagcggttaa acaaacacct aatgcaactg acgaagaaaa gcaggctgca
      781 gttaatcaaa tcaatcaact taaagatcaa gcgtttaatc aaattaatca aaaccaaaca
      841 aatgatcagg tagacgcaac tacaaatcaa gcgattaatg ctatagataa tgttgaagct
      901 gaagtagtaa ttaaaccaaa ggcaattgca gatattgaaa aagctgttaa agaaaagcaa
      961 cagcaaattg ataatagtct tgattcaaca gataatgaga aagaagttgc tttacaagca
     1021 ttagctaaag aaaaagaaaa agcacttgca gctattgacc aagctcaaac gaatagtcag
     1081 gtgaatcaag cggcaacaaa tggtgtatca gcgattaaaa ttattcaacc tgaaacaaaa
     1141 attaaaccag cagcacgtga aaaaatcaat caaaaagcga atgaattacg tgcgcaaatt
     1201 aatcaagata aagaagcgac agcagaagaa agacaagcgg cgttagataa aatcaatgat
     1261 ttagttgtta aagctatgac aaatatcacg aatgatagaa caaatcagca agttaatgac
     1321 tcaacaaatc aagcgcttga cgacattgca ttagtgacgc ctgaccatat tgttagagca
     1381 gctgctagag atgcagttaa gcaacaatat gaagctaaaa agcacgaaat tgagcaagcg
     1441 gaacatgcga ctgatgaaga aaaacaagtt gctttaaatc aattagcgaa taatgaaaaa
     1501 cgtgcattac aaaacattaa tcaagcaata gcgaataatg atgtgaaacg tgttgaatca
     1561 aatggtattg ctacgttaaa aggcgtagaa ccgcacattg tggttaaacc tgaagctcaa
     1621 gaagccataa aagcgagcgc agataaccaa gtagaatcta taaaagatac accacatgct
     1681 acgacagatg aattagatga agcaaaccaa caaataaacg acacacttaa acaaggtcaa
     1741 caagatatag acaatacgac acaagatgca gctgtcaatg atgttagaaa ccaaacgatt
     1801 aaggcaatcg aacaaattaa accgaaagtt agacgcaaac gtgcagcgtt ggataacatt
     1861 gatgaaagta ataataatca actcgatgca atacgaaata cgctagatac aacgcaagat
     1921 gaacgaaatg ttgctattgc tgcgttaaat aaaattgtta atgcaattaa aaatgatatt
     1981 gcacaaaaca aaacgaatgc agaagtggat caaactgagg ctgatggtaa caacaacatc
     2041 aaagtgattt tacctaaagt tcaagttaaa ccagcagcgc gtcaatctgt cagcgcaaaa
     2101 gctgaagctc aaaatgcact tattgatcaa agtgatttat ctaccgaaga agaaagatta
     2161 gctgctaaac atttagtaga acaagcactt aatcaagcta ttgatcagat caatcacgca
     2221 gataagactg cgcaagttaa tcaaaatagt atcgatgctc aaaatattat ttcaaaaatt
     2281 aaaccagcga caacagttaa agcaacagca ttacaacaaa ttcaaaatat cgctacaaat
     2341 aaaattaatt taattaaagc aaataacgaa gcgacagatg aagaacaaaa tgctgcaata
     2401 gtacaagttg aaaaagagtt aattaaagct aaacaacaaa ttgctggtgc agtgactaat
     2461 gctgatgtgg catatttatt gcatgatggg aaaaacgaaa ttcgtgaaat cgaacctgtt
     2521 attaataaaa aagcaactgc gcgagaacaa ttaacaacat tattcaacga taagaaacaa
     2581 gcaattgaag cgaatgttca agcaacagta gaagaaagaa atagtatttt agcacagtta
     2641 caaaacattt atgacactgc tattggacaa attgatcaag atcgtagcaa tgcacaagtt
     2701 gataaaacag caacattaaa tctacaaaca atacatgatt tagacgtaca tcctattaaa
     2761 aagccagatg ctgaaaaaac gattaatgat gatcttgcac gtgttacaca tttagtgcaa
     2821 aattatcgaa aagtaagtga tcgtaataag gctgatgcat taaaagctat aactgcatta
     2881 aaattacaaa tggatgaaga attaaaaaca gcacgcacta atgctgatgt tgatgcagtt
     2941 ttaaaacgat ttaatgttgc attaggcgat atagaagcag taattactga aaaagaaaat
     3001 agcttactgc gcattgataa cattgctcaa caaacatatg cgaaattcaa agcgatcgca
     3061 acaccagaac aattagctaa agtaaaagca ttaattgatc aatatgttgc agatggcaat
     3121 agaatggttg atgaagatgc gacattaaat gacatcaaaa aagatacgca actcattatt
     3181 gatgaaattt tagcaattaa attacctgct gaagtgataa aagcgtcacc aaaagtgggg
     3241 caacctgctc caaaagtttg tacgcctatt aaaaaagaag ataaacaaga agtgcgaaaa
     3301 gttgtaaaag aacttccaaa tactggttct gaagaaatgg atttaccatt aaaagaatta
     3361 gcactaatta caggcgcagc attattagct agaagacgtt ctaaaaaaga aaaagaatca
     3421 taataaacaa aaagacctta atataacgac actttttagt gactcagtta tattaaggtc
     3481 atttttaatt gaatgaaaag gttgatctac tagagagaat cgaacccaat tttatactat
     3541 tattccattg atgcatgtga gtaatgaccg gcatataatc cagcgacatg tcctgttacg
     3601 agtgcacttg taatattata accaccagta taaccatgta tgtctaatac ttcaccacat
     3661 aaaaataatc caggaactaa tttagacatc atggttttag gttgaatttc ttttagtgac
     3721 acaccaccac ctgtgacaaa tgccttatct ataggtaatg tcccgtttac cttaaataca
     3781 aaccctttaa acatatttac taagtcgttc aattgttgat ttgataagtg atgtgatgtg
     3841 gtattttcat cgatttcagc ttgttccagc atgaacaata agtagcgctc ttcaattagt
     3901 ccatgcaagc tgtttttaat gattttatct ggtgtgtccg ataataatga tgtgatgtgt
     3961 tgttttaatt gttcatggtt taattcagga aatgtatcga ttgccattga aatatgctgt
     4021 gtcttttgat ttttttgttc tttataaaca aactgactac atcttaatgc agctggacca
     4081 ctgataccaa aatgagtaaa taacatatcc atttgatgac tgatgcgttt tttaccattt
     4141 ttcttaagta ctgacaattc aacatctttt aaacttaagc cttttagacg attggattta
     4201 atgaaaggtt cagctgatgt aattggaact tcggtcggga ataactccgt aatggtatga
     4261 cctaaatctt gtgcaaactt ataaccatca cctgttgaac cagtttgagg gacacttgta
     4321 ccacctgtag caattactag cgaatggctt tcataactat tattttgagt atgtacagtg
     4381 aaagtttggt ctgtattaac ttcgattcta ctaacagctt cttcttcttt aatcgttaca
     4441 tgttgatttt ctatagttgt cactaaagta tcaacgacgt cttgtgcctt attggaaact
     4501 ggaaacatac gaccgtgatc ttcttctttt aatttaacac ccctagactc aaaaaaatct
     4561 atgatggatt cattatcaat aattgaaaag ggactatata aaaatttccc atttccagga
     4621 atgttcttaa taatttcagc atagggtaat cgattagtta cgttacatct accgccacca
     4681 gatattttga gtttacgacc tagacctttc tttttttcga tgagtagaac gttgtcattt
     4741 tgttcgcttg cagctactgc cgccattaag ccgctaggtc cgcctccgat aataattgtt
     4801 tgatacatat acgcttcctc caaatttgtt actggacatt caattgctat tttatagtac
     4861 ttttttaaac tttgctatat ctcttgtgtt atgaaatgac actcgcgctg aatatattta
     4921 taatgtgtgt ttcataaaag gataagcatt tattcgaacg ctattattat tttaagagtt
     4981 gtccgccatt tattggcatt agtagtataa tgacaatatg aaaatttatg aatttcaaag
     5041 caactttgag tattcagtga caacacgtta ttatttttgt tatgatattt cattgaatat
     5101 acgacaatga aataaagttt tgtgatatat tcataataat gaaaatgatt ttacagagaa
     5161 atagcttttt atttacaaaa cattaataaa catgagatag ggagatacgt aatgagtgaa
     5221 agtaaagaaa tggtgcgtgg gaccttttta attacaatta gtattttgat tacaaaggtg
     5281 ttaggcgtac tttttatcat tccattcaac tatttaattg gtggacaaga aaatatggcg
     5341 ccgttcacat acgcttatgc accatataat attgcaattg cagttgcgac agcaggtgta
     5401 ccattagctg cttctaaata tgtagcgaag tataatgcga ttggtgcata taaagtcagt
     5461 cagaaatttt ataaatctag ttttattgtc atgagtatta ccggtgtttt aggtttctta
     5521 gtactttatt tcctagcacc atatatttct gaattaactt tagctagaaa tgttcacgat
     5581 aaaaatggtt ggtcagttga tgacattaca tggattatta gaatcattag tatggtagtc
     5641 atcttcattc ctgtattagc aacatggaga ggtatattcc aaggttataa atctatggga
     5701 cctactgcgg tatcggaagt aactgagcaa attgcacgcg tgattttcat attaatcgga
     5761 agttatttag tactaaatgt ctttgatggt tctatcttat tagccaatgg tattgctact
     5821 tttgcagcag cagttggtgc gattattggt atattcacac tctggtatta ttggagaaag
     5881 cgtaagcata atattgatcg tatggtcgaa tctgattaca cagatataga tgtgtcatat
     5941 ggcaagatgt ataaagaaat tattgcttat agtattccgt tcgttattgt aagtttgaat
     6001 tatccattat ttaacttagt agatcaattt acacataacg gtgctttatc tttagttggt
     6061 ataccttcac agttacaaga tatattcttt aatatgctaa atatgtcaac taataaaatt
     6121 gtaatgattc cgacatcatt aagtgctggc tttgcagtaa gtttaattcc ttatattaca
     6181 aaaacatttg cagaaggtcg cttacatgaa atgcatcatc aaattagaac atctattggt
     6241 gtattaatgt ttataactgt tccagctagt attggaatta tggccttggc acaaccgtta
     6301 ttcacagtat tctatggtta cgatccaatt gttttaggtc atgaccctaa ccatgatggt
     6361 agtcgtttgc tattctatta tgcgcctgta gcaatattaa tttcactatt aagtgtaacg
     6421 gcgtcaatgt tacaaggtat cgataagcag aagttaacgg tttatgttat tttagcttct
     6481 gttgcaatta aattagcatt aaactaccca ttaattatgc tattccatac gccgggggct
     6541 gttttaagca caagtatcgc attattattc gcaattggtt gtaacttcta tattcttaaa
     6601 aaatacgcga aatttaaatt tagctatagc tggattcatt tcgcaaaaat attcttatac
     6661 tcatttatta tgatgttagg tgtagaagca gtattcttca ttgcgaacct attcttagag
     6721 ccaacaaaac ttggatattt aattattatc attttaggtg taacagttgg tattctaatt
     6781 tacggtacaa tcacaattaa aacgcgtctt gcggacgaat tcttaggtga aattcctgaa
     6841 aaattaagac gtagagttag gtttttacga tgagaataga taaattttta gcaaatatgg
     6901 gcgtcggaac acgtaatgag gtcaaacagt tacttaaaaa aggccttgta aatgtcaacg
     6961 aacaagtaat taaatcacca aaaacacata tagaaccaga aaacgataag atatcagttc
     7021 gtggagaatt aatagaatac gttgaaaatg tctatattat gttaaataag ccaaaaggtt
     7081 atatatcggc aactgaagat catcattcaa aaaccgttat tgatttaata cctgaatacc
     7141 aacatttaaa tatttttccg gttggtcgtc ttgataaaga tacagaaggg cttttattga
     7201 taacgaatga tggtgatttt aatcatgaat taatgagtcc aaacaaacat gtttctaaaa
     7261 aatatgaagt tatttcagca aatcctatca ctgaagaaga cattcaagct tttaaagagg
     7321 gtataacact gtctgatggc aaggttaagc ctgcaatatt aacttatatt gataatcaaa
     7381 cgtcacacgt aacaatttat gaaggaaaat atcatcaagt taaacgaatg tttcatagca
     7441 ttcaaaatga ggtattacat ttaagacgta tcaaaattgc tgatttagaa ttggatagca
     7501 atttagattc aggagaatat cgtttattaa ctgaaaatga ttttgacaaa ttaaattata
     7561 aataaaaggg agacgattga tatggcaaaa ggtaatttat ttaaagcgat tttaggtata
     7621 ggtggcgctg tagcagctgt acttgttaca cgtaaagata gtcgtgacaa gctgaaagca
     7681 gaatataata aatacaaaca agatcctcaa agctataaag ataatgctaa ggataaagcg
     7741 acgcaattag gaacaattgc aaatgaaaca attaaagaag taaaaacaaa tccgaaagaa
     7801 tatgctaata gattaaaaaa taatccaaaa gcatttttcg aagaagaaaa atcaaaattt
     7861 accgaatatg acaataagac tgacgaaagt attgaaaaag gtaaatttga tgatgaaggt
     7921 ggcgcagcac caaataataa tttacgtatc gtcactgaag aagatttaaa aaagaataaa
     7981 aatgcattgt ctgataaaga ataaggcata caattaataa gttggtattg aactggcata
     8041 ttaaattaaa ttaatacaga aaaggaacaa agcagttatc attaatttta tgttaaatcg
     8101 atagcgttgt tttgttcctt tttattattt tctgacatct tattgtagtt tttatatcat
     8161 ttcatgtaaa ataggattta acttatagaa aaaaggaagt cgatttcgaa tgtggaaaga
     8221 aaaagttcaa caatacgaag atcaaatcat taatgactta aaaggattat tagcaattga
     8281 aagtgtgaga gatgatgcaa aagcatcaga agacgcacca gttggtccag gtcctcgtaa
     8341 ggcattagac tacatgtatg aaattgcaca tagagatgga tttacaacac atgatgtgga
     8401 tcatattgca ggaagaattg aggcaggtaa aggaaatgac gtattaggta tcttatgtca
     8461 tgttgacgtt gttcctgctg gtgatggatg ggatagtaat ccgtttgagc cggttgtaac
     8521 agaagatgct atcatagcta gaggtacact tgatgacaaa ggtccaacaa ttgctgctta
     8581 ttatgcaatt aagatattag aagatatgaa tgtggattgg aagaaacgta ttcatatgat
     8641 tattggtacg gatgaagaat ctgattggaa atgtacggat cgctatttta aaacagaaga
     8701 aatgccaaca ttaggttttg caccagatgc agaatttccg tgtattcatg gtgaaaaagg
     8761 cattacaaca tttgacttag ttcaaaataa gcttgctgaa gatcaagatg aacctgatta
     8821 tgaattaata acttttaaat ctggtgaacg ttacaacatg gtacctgatc atgcagaagc
     8881 aagagtgctt gttaaagaaa atatgacaga tgttattcaa gactttgagt attttttaga
     8941 acaaaatcat ttacaaggtg atagtactgt tgatagtggc attctagttt taacagttga
     9001 aggtaaagcg gttcatggta tggacccatc tatcggtgtg aatgcgggtc tttacttact
     9061 aaaattctta gcatcattaa atcttgataa taatgcaaaa gcgtttgtag catttagtaa
     9121 tcgctactta tttaattcag attttggtga aaagatggga atgaaattcc atacagatgt
     9181 catgggtgat gtgacaacta acattggtgt tattacatat gataatgaaa atgcgggtct
     9241 tttcggtatc aacttgcgct acccagaagg atttgaattt gaaaaagcta tggatcgttt
     9301 tgcaaatgag attcaacaat atggctttga agtgaaatta ggtaaagtcc aaccaccaca
     9361 ttatgttgat aaaaatgatc cttttgtaca aaagttagtt actgcatata gaaatcaaac
     9421 aaatgatatg actgaacctt atactatagg tggcggtact tatgcgagaa acttagacaa
     9481 gggtgtagca tttggcgcaa tgtttagtga ttctgaagat ttaatgcatc agaaaaatga
     9541 atatatcact aaaaaacagt tatttaacgc aactagtatt tacttagaag caatttattc
     9601 attatgcgtg gaggaataat atatggaaaa aattttttta aatggtgagt ttgtaagtcc
     9661 aagtgaagca aaggtttcat acaacgacag aggatacgta tttggcgatg gtatttatga
     9721 atacattcga gtatacaatg gtaagttatt tacagtaaca gaacattatg aaagattttt
     9781 acgtagtgcc aatgagattg gtttagattt aaattattct gtagaagaat taattgaact
     9841 atctcgtaaa ttagttgata tgaatcaaat tgaaactggg gcaatttata ttcaagcaac
     9901 gcgaggtgta gctgaaagaa atcatagctt cccgacacct gaagtagaac cagcaattgt
     9961 tgcttatact aagagttatg atcgtcctta tgatcattta gaaaatggtg tgaatggtgt
    10021 taccgttgaa gatatccgat ggttacgttg cgacattaaa agcttgaacc tattaggaaa
    10081 tgtattagca aaagaatatg ctgtgaaata taatgcagtt gaagcaatcc aacatcgagg
    10141 cgaaactgtc actgaaggat cttcaagtaa tgcttatgca attaaagacg gtgtgattta
    10201 tacacatccg attaacaact atattcttaa tggtattacc cgaattgtaa ttaaaaaaat
    10261 tgccgaagac t
//
LOCUS       DAMZPP010000110         9941 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMZPP010000110 DAMZPP010000000
VERSION     DAMZPP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9941
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.110"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(50..1366)
                     /locus_tag="POH73_002068"
     CDS             complement(50..1366)
                     /locus_tag="POH73_002068"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDI6888000.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRKYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEIDAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYVIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(1418..2242)
                     /locus_tag="POH73_002069"
     CDS             complement(1418..2242)
                     /locus_tag="POH73_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI6888001.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIERNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            2356..2664
                     /locus_tag="POH73_002070"
     CDS             2356..2664
                     /locus_tag="POH73_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDI6888002.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
     gene            3244..5058
                     /locus_tag="POH73_002071"
                     /pseudo
     CDS             3244..5058
                     /locus_tag="POH73_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
     gene            5261..7870
                     /gene="clpB"
                     /locus_tag="POH73_002072"
     CDS             5261..7870
                     /gene="clpB"
                     /locus_tag="POH73_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDI6888003.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDSDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(7929..8798)
                     /locus_tag="POH73_002073"
     CDS             complement(7929..8798)
                     /locus_tag="POH73_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6888004.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSIND
                     VHTSIKLIEMNQGISFLPIYITKNSNYNISVINTKILQAPISFTYIYSKKENPEILAF
                     IKSFKKYIANEQL"
     gene            8909..>9941
                     /locus_tag="POH73_002074"
     CDS             8909..>9941
                     /locus_tag="POH73_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDI6888005.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHI
                     KEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDATRTSREKLKEYMEIISKYQVRT
                     VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIE
                     TTFLGIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPI
                     IGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPISNISSKKILHNKFK
                     SIVNTEEIDE"
BASE COUNT         3518 a         1369 c         1723 g         3331 t
ORIGIN      
        1 caaaattcta acctagacaa ttaagattag attgaatcgc atgattcatt tatttagctt
       61 tgtaaccaat catattgatt aaatctttag ggtggctata tggtggtgca taagccactt
      121 caaactcagt taactcatct acagttagct ggttcatcat tgccatcgat agtacatcaa
      181 tacgtttatc tgcgccttct tttcctactg cagctgctct taaaatctga cggtttgaag
      241 tgtcataata tactcttaag tgtaaagggg aatttcctgg gtaataattc gcgtgtgcac
      301 cttgagtgac ttccaccatt ttatagtcaa attgctttag ttcgtttggt ttaacgccga
      361 cactcgcaaa tgtataatca aagaacttca caatattgtt gcctaagaag cctttgaatt
      421 caatagtgtc atttccagca atttgttcgg caacaatact tgctgcacgg tgagcgcccc
      481 aagctaaagg aacactagcc ggtagatcga catgtcgata atgtgatgtt gcaatatcgc
      541 ctattacata aatgtttgga acatttgttt caaatttatc gtttaccggt atgaaacctt
      601 ttcgatcaag tttgatattt gaactttcga taaattttga attggggtga gtaccgacac
      661 cttcaataat catatcgtaa tgttcaactt ttcctgattt aaatgtaatt tcatttccat
      721 tgatagcatc aatttcctca tttaaacggt atggaatctc ccgcttatct aattcatcaa
      781 gtataggttg attcatatcg gcatccatta atttatttat cttatcagat cgatgaatta
      841 aagtagggtg taaaccacgt tcataaagat tttcaagaac ttctaatgaa acataccctg
      901 cacctacaac caatacttta tcaacttgat ttgctttgat gaattgatcg atagcatcag
      961 tgtcttctaa atttctaagt gtaaatgtaa tatcactttc aaagccaagg ctatttgcac
     1021 ttgcaccagg gcttaaaatg agtttatcgt atgattcttc aaattgttcg ttggtctttc
     1081 tatttaatac agatacagtt tgtctttcat cattgattgc aataacttca tgataagttt
     1141 ttactgtaat ttgttttcta tcataaaatt tttcaggtgt atacgctaaa gcatattttc
     1201 tatcttcaac aacttcgcca atgacataag gcaatgcaca attagcaaag ctcatatcac
     1261 gatctttttc aaaaataata atgtcacttt ctttatctaa acgtcgaatt tggctggcac
     1321 atgttgctcc gccagcgact gctccgacta cgactatttt gggcatcgta acttacctcc
     1381 tattgtttta aaatattata aaatgattag ttataaatta acagtaatat ctaatattta
     1441 aattaaagaa atcatttaaa tatcgaccaa ttccatcttc attattgttg aatgtaatat
     1501 tgttcgctac atctttaagt tcttgcaaac cattttccat agcaacaccg tgacgcgcgt
     1561 actcaatcat ttctatatca ttatcttcat caccgaatgc aataatatta tttcgttcaa
     1621 tatttaaaaa ttgtctaact tgttcaatgc ctcttgcttt attaattcca agttttacaa
     1681 tttcaatgac agggaatggt gcgccccagc gtcgatgctc aatatgatcg gcataaaaat
     1741 gagtaagcat atttttgatt tcaggtattt tactttcttc ggcttcaatt aaaattgagg
     1801 taggggattc tttcaagtgg acaagtaaat taccagtttg aattcttgga ttacccattg
     1861 aaaaaccttc aaataatctt ggatcatgat tattaatgaa aacataatct ttcacttctg
     1921 caataatatt cgatacttga tattgttgta atccttgaat aatattttgt gcgatgccta
     1981 aatctaaaat ttcatggcaa gttttgaagt ttttatcttt agggtgatgt acgtaagcgc
     2041 cattaaaatt aacaattggt gtcgttaaat ttaattcatg ataatacatt tgacttgcac
     2101 gataaggtct gccagtcgca atcataattt ggtgtccacg ttgttgtaat tcatttaata
     2161 cttgtttagt atatgatgaa atttctttgt tatcgtttaa taatgttccg tctaagtcta
     2221 gacatattaa atgtggttgc atatctctct cctcataagt tgcttagttc atatagatac
     2281 atcatagcat tttattggcg ttttgtcgga tatttgatat attatgaata atatgacatt
     2341 aaagaggtga tatggatgga agaggcattg aaagatagta tcttaggtgc attagaaatg
     2401 gtaattgacc ctgaattagg aattgatatc gttaatttag gtttagtata caaagtgaat
     2461 gttgatgatg aaggcgtatg tacagttgat atgactttaa catcaatggg atgtccaatg
     2521 ggacctcaaa ttattgatca agttaaaaca gtattagcag agattcctga aattcaggat
     2581 actgaagtga atatcgtatg gagtccacct tggacaaaag atatgatgtc acgttacgct
     2641 aagattgcac ttggtgtgag ctaacataaa cttgcattac ttgtagaaat tggaaatcca
     2701 attggggctc cgctcccaac ttgcattgct tgtagaattt cttttcgaaa ttctctgtgt
     2761 tggggccccg cagtcaatat aacattgtag agtataattc attgtgaata agctcccttc
     2821 aaagtagaca ttgaagggag ccttatttac gttctaggtt ctgactttat tttgaaaatt
     2881 tatatctaac ttgtttcaga aaatatgaat taagacagtg acttgcactt ctaagagtgc
     2941 acaattcatt tcctatttga atattttctt gaaaatttct ataataaatt gaatttatat
     3001 tacagttata ttacaataca taatacttca tagtttttaa gttgccgagt tgcattacaa
     3061 attggatttc gaaataagaa tcatcattgg taatttttta atttaacagt attttagtca
     3121 atatgatttt gatgatgtcg taattcaata atatacatat gcatttcaat attcttttaa
     3181 acagcataac taaatttaat taagcatcta agaagaacag ttttaaaagg gaagaatgca
     3241 aaaatgaaca aaacaaaggg ttttacaaaa tataagaaaa tgagatatat gccagggctc
     3301 gatggtttga gggcaatcgc tgttctagga attattattt accacttaaa taagcaatgg
     3361 ttgacaggtg gctttttagg tgtggataca ttttttgtga tctctggtta tttaattaca
     3421 agcttattac tcaaagagta tgatgacaca ggtatcatta aattgaaaag cttttggata
     3481 cgtcgtttaa aacgtttatt accagcagtc atagttttat taatggttgt agggacagca
     3541 accttattat taaaatcaga taatatcatt agggttaaac atgatattat tgctgcgata
     3601 ttttatgtat caaactggtg gtatatagca aaagatgtta attattttga gcaattttca
     3661 tttatgccat taaagcattt atggtcttta gcaattgaag aacagtttta catatttttc
     3721 ccagttattt tggttacatt attgttaaca attaaaaagc gatacaaaat aggatttatt
     3781 ttttggggag tatctataat ttctttaggg ttaatgatgt ttatctacag tattaatggg
     3841 gatcattcac gagtgtattt tggtacagat actagattac agacattgtt actgggtgtt
     3901 attttagctt ttttatggcc gccgtttaaa ttgaaaaatg atccacctaa agttgtaaaa
     3961 tatgttattg atagcatagg tagtttatca tttatagtac ttatattatt atttttcatt
     4021 attaatgatg agacgaattg gatatatgat ggtggtttct atttaatatc catattaacg
     4081 ttatttatta ttgccagtgt cgttcatcca tctacatgga tagcgaagat attttcaaat
     4141 ccagtgttag tatttatcgg gaaaaggtct tatagtctat atttatgaca ttttgcagta
     4201 attagtttcg tacatagtta ctatgtagac ggacagatac ctgtatatgt gtactttata
     4261 gatataagtt taacaattat atttgcagag ctatcatatc gctttataga aactccattt
     4321 agaaaagaag gtattaaagc tttaaattgg cgatcttctt atataccaca atttataaga
     4381 atggtaattg tagtaacctt gttaattcca tttatgttga ttttagtagg tgcattcaat
     4441 aaatatggta aagacattat aggagaaaaa gcgaatagct ttgataccac tattgaagat
     4501 aattattcaa tgcggatagc accaattgat aacattcaca ttgatggctt agtaagtgag
     4561 aagaaaaagg aatcttccga tgtatataat aatattaaac ctcttttaat cggtgattca
     4621 gtaatggttg atatcggtga gtcatttaag tcatcagttc ctaagtctag aattgatgga
     4681 aaagtagggc gtcaattgta tcaaacctta cctttagtta aagcgaatta ttcacaatat
     4741 aaaaaatcat ctgatcaagt cgtattagaa ttaggtacaa atggcgactt tactgtcaaa
     4801 cagctcgacg atttacttaa tcaatttgga aaagccaaga tttatttagt taatacacgt
     4861 gttccaagaa tttatgaggc aaatgtaaat cgattattag ctgacgcggc gaaacgaaag
     4921 tccaatgtca cattaattga ttggtataag cgatcacaag gacatagtga atattttgca
     4981 ccagacggtg taaatttaga gtacaaagga gtcctagctt taaaagatga aatattaaaa
     5041 gcacttaaaa agaaataaca tatgtttaat aagtctagtt aatgtgtaac gtaacattag
     5101 ctagattttt ttaattcaaa aaattattta caaatattag gaaatttaag tgtaaaagag
     5161 ttgataaatg attatattgg gactataata taattaaggt caaagaaagt caaagtcaaa
     5221 agtgcaactt gaccaactct acaaattaga ggtgaaatta atggatataa ataaaatgac
     5281 atatgctgtt caaagtgctt tacaacaagc agttgaactg agtcagcaac ataaattaca
     5341 aaatatagaa attgaggcaa ttttaagcgc tgccttaaat gaaagtgaaa gcttatataa
     5401 aagtatttta gaacgagcaa atattgaggt agatcaatta aacaaagctt atgaagacaa
     5461 actaaacacg tatgcatctg tagaaggtga caatatacaa tatggtcaat atattagcca
     5521 acaagcaaac caattgataa ctaaggctga atcatacatg aaagaatatg aagatgaata
     5581 tatttcaatg gagcatattt tacgttcggc aatggacatt gatcaaacaa caaaacatta
     5641 tataaataat aaagtagaag ttatcaaaga aattattaaa aaagtaagag ggggaaatca
     5701 cgtgacatca caaaatccag aagttaatta cgaagcatta gctaaatatg gccgcgactt
     5761 agtagaagaa gttagacaag gtaaaatgga tcctgttata ggaagagatg aagaaattcg
     5821 aaatacgatt cgtattttaa gtcgtaaaac taaaaacaac cctgtgctca ttggtgaacc
     5881 aggtgttggt aaaactgcaa ttgttgaagg attagcgcag cgtatagtta agaaagatgt
     5941 gccagaatca ttattagata aaactgtttt tgagttagat ttaagcgcat tagtagcggg
     6001 cgctaaatat cgtggtgaat ttgaagagag attaaaagca gtcctaaaag aagttaaaga
     6061 gtctgatggt agaattatat tatttattga tgaaatccat atgcttgtag gtgctggtaa
     6121 aacagatggt gccatggatg caggcaacat gttaaaacca atgttagcac gaggagagtt
     6181 acattgtatt ggtgcaacaa ctttaaatga atatcgagaa tatattgaaa aagattcggc
     6241 attagagcgt cgtttccaaa aagtagcagt tagtgagcct gatgttgaag atacaatttc
     6301 aattttacgt ggtttaaaag aacgatatga agtatatcat ggtgtgcgta ttcaagatag
     6361 agccttagtt gctgccgctg aattgtctga tcgttacatc actgatcgtt ttttaccaga
     6421 taaagcgatt gatttagttg accaagcatg tgcaacaatt cgtacggaaa tgggatcaaa
     6481 tccaactgaa ttggatcaag ttaatagacg tgtcatgcaa ttagaaattg aagaaagcgc
     6541 acttaaaaat gaatctgaca atgcgagcaa acagagatta caagaactac aagaagagct
     6601 tgccaatgaa aaagagaaac aagcagcact tcaatctcgt gtagaatcag aaaaagaaaa
     6661 aatagcaaat ttacaagaaa aacgtgcgca actagatgaa agtagacaag cgttggaaga
     6721 tgcacaaaca aataacaatt tagaaaaagc agctgaacta caatatggaa caattcctca
     6781 attggaaaaa gagcttagag aattagagga taatttccaa gatgagcaag gtgaagattc
     6841 agatcgaatg attcgtgaag ttgtaacaga cgaagaaatt ggtgatattg tcagccaatg
     6901 gacaggcata ccagtttcaa aattagttga aacagaacgt gaaaaattac ttcacttaag
     6961 tgatatcttg cataaacgtg ttgtaggtca agataaagcg gttgacctgg tttcagatgc
     7021 agtagttaga gctagagcag gtattaaaga tccaaacaga cctattggta gtttcttatt
     7081 cctaggtcca actggggtag gtaaaactga attagctaaa tcattagctg catcattatt
     7141 tgattctgaa aaacatatga ttcgtattga tatgagtgaa tatatggaaa aacatgcagt
     7201 atcaagattg ataggggcac ctccaggata tattggacat gatgaagggg gtcaattaac
     7261 tgaagcggtt cgtcgtaatc catactcagt tattttatta gatgaggttg aaaaagcgca
     7321 tactgacgtc tttaatgtat tattgcaaat tttagatgaa ggccgtttaa ctgattctaa
     7381 aggacgtagc gttgatttta aaaatactat tattattatg acaagtaata ttggatctca
     7441 agttttacta gaaaacgtaa aagagactgg tgaaattaca gaatcaacag aaaaagctgt
     7501 tatgacaagt ttaaatgcat atttcaaacc agaaattttg aatcgtatgg atgacatcgt
     7561 attatttaaa cctttatcta ttgatgacat gagtatgatt gtagacaaaa tattaacgca
     7621 attaaatata agattactag aacaacgaat ctcaattgaa gtttctgatg atgctaaagc
     7681 ttggctaggt caagaggctt atgaacctca atacggtgca agaccattaa aacgttttgt
     7741 acaacgccaa attgaaacac cattagcacg tatgatgatt aaagagggat tcccagaagg
     7801 tacaactatt aaagttaatt taaattcaga caataactta acgtttaatg ttgaaaaaat
     7861 tcatgaataa aattgaacca agaatgtgat gattaatcgc attcttggtt ttttagattt
     7921 gaaatttatt ataattgttc attggcaata tactttttaa atgatttaat aaacgccaat
     7981 atttctggat tttctttttt gctgtatata tatgtaaatg aaataggtgc ttgtaaaatt
     8041 ttcgtgttaa taaccgaaat attgtaatta ctattttttg ttatataaat aggcaggaat
     8101 gatatacctt gattcatttc gattaattta attgaagtat gcacatcatt gatagataga
     8161 aattgtgctt tttcataaat atttaaaata ttatttttaa gtgatgacca atattctgga
     8221 tggttatcac ttattatttt gtatttttca aataaagatg cctcagttag aagatgatta
     8281 ttctctttat tgggagcaat caatacaatt ttaccttcgc atactttttc agaatgaact
     8341 tctcttagtt taggttgatt tctgctaatc ccgatgtcat acgtatgatt attaatatct
     8401 ttttcaatat tttcattttt gacatggaga gaaacatcga taaacggatg ctcgttaaag
     8461 aaagatttta aaaatttggg cataatgaat gtcgcgatat atgaagacac gacaacattt
     8521 aatttcgatt gaaacatcgt ctttttaagt tggatatgtt tgatgccact ttcatattgt
     8581 tcaataaaac tttgcgcaat tggaagaaat gtatgaccat cttcagtcaa gataatttga
     8641 tttttataag tttcaaatag tttcacattg agatgctgtt ctaaattttt aatttgctta
     8701 tgtatagaag gtatagtgag attaatttct tcactagcta atcgatagtt taacgtcttc
     8761 gctaaaatga caaatgtata gtaccaatcc aaattcatga acttacctct ttcaaaaaaa
     8821 gttaataatt cattagataa tatttaattt ttattgcata aaaataatat taatattata
     8881 tcagaggtta tcgagggggt gaagtaatat gattcgaatt caagataata caataaggga
     8941 tggtatgcaa caaagtaatg ttcgaaaaag tctaattata aaaaaagaag tattgaaaca
     9001 aattaacaag ttaaatataa attctgttga agtaggcatg tgtacaacta tcgaggatga
     9061 atttaatatt catcaattca gagacatttt aagtcctgaa aaagaattag tagtattgac
     9121 taggcttaat gaaaaagaaa taaaaaaaat agtcaaatta aaaattcata atttagtggt
     9181 aaaaatacta ttgccaatat ctgacttgca tataaaagaa aagcttaatt tttcaaataa
     9241 atattatatt cagaaaatca aagactgctt ggatatatta aagaaagata aaaaaggagt
     9301 agatatttgt tttgaagatg caacaaggac ttctagagaa aaattgaaag aatacatgga
     9361 aattatttca aaatatcaag ttagaacagt tacatttgcg gacactgtag gatgttcgac
     9421 accattagaa tacggagata tttttaatta ctttgtaaaa aaatattcta acataatttt
     9481 ttctgctcat tgtcataacg atctagggtt ggctactgca aatacattag ctgcaatttt
     9541 aaatggtgcg aagcaaatag aaactacatt tttgggaatt ggtgagagag cgggtaatgc
     9601 tcctattgag gaaataatta ctattttgac aaaaaaacaa atagaaagta cggaattcac
     9661 tttacccgac gtatataaaa ctagtattaa tatttctaaa attctcgatt ttcaaatatc
     9721 agaaaacaaa cctataattg gtgaaaatat atttaaacat gaatcaggaa ttcatcaaga
     9781 tggtactaaa aaaaatataa atatgtatca atatttagtt cctagtgatt taggattgaa
     9841 aaattcacaa gttgttcagt ttccaataag taatatttct agtaagaaaa tcttgcacaa
     9901 taaatttaaa tcaatagtta acactgaaga aattgatgaa a
//
LOCUS       DAMZPP010000111         9794 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMZPP010000111 DAMZPP010000000
VERSION     DAMZPP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.111"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..233
                     /locus_tag="POH73_002075"
     CDS             <1..233
                     /locus_tag="POH73_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDI6888006.1"
                     /translation="SVEKTGRAVVVQEAQRQAGVGAAVVAELSERAILSLEAPIGRVA
                     AADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            324..1616
                     /locus_tag="POH73_002076"
     CDS             324..1616
                     /locus_tag="POH73_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDI6888007.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATNEEVAETPAAPAAVSLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            1620..3026
                     /gene="lpdA"
                     /locus_tag="POH73_002077"
     CDS             1620..3026
                     /gene="lpdA"
                     /locus_tag="POH73_002077"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDI6888008.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            3197..3472
                     /locus_tag="POH73_002078"
     CDS             3197..3472
                     /locus_tag="POH73_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDI6888009.1"
                     /translation="MEYEYPIDLDWSNEEMILVINFFNHVEKYYESGVTAGDFMDAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            3616..4155
                     /locus_tag="POH73_002079"
     CDS             3616..4155
                     /locus_tag="POH73_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6888010.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEVYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            4168..5262
                     /locus_tag="POH73_002080"
     CDS             4168..5262
                     /locus_tag="POH73_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6888011.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            5255..6052
                     /locus_tag="POH73_002081"
     CDS             5255..6052
                     /locus_tag="POH73_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6888012.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVSVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAALITIITLAISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            6058..6867
                     /locus_tag="POH73_002082"
     CDS             6058..6867
                     /locus_tag="POH73_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6888013.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRKKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            6867..7940
                     /locus_tag="POH73_002083"
     CDS             6867..7940
                     /locus_tag="POH73_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6888014.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDKGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLEQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKMSLK"
     gene            8014..9033
                     /locus_tag="POH73_002084"
     CDS             8014..9033
                     /locus_tag="POH73_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDI6888015.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQELERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            9316..9735
                     /locus_tag="POH73_002085"
     CDS             9316..9735
                     /locus_tag="POH73_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDI6888016.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVCL
                     MALNLIAIVLWIIVMIMLISKKESKETTHKDDEYIYH"
BASE COUNT         3532 a         1340 c         1815 g         3107 t
ORIGIN      
        1 cttcagttga aaaaactggt cgtgcagttg tagttcaaga agcacaacgt caagctggtg
       61 ttggtgcagc agttgtagct gaattaagtg aacgtgcaat cctttcatta gaagcaccta
      121 ttggaagagt tgcagcagca gatacaattt atccattcac tcaagctgaa aatgtttggt
      181 taccaaacaa aaatgacatc atcgaaaaag caaaagaaac tttagaattt taatacattg
      241 taaaagttaa cgaagttagc gtattttagt ctcattgatt aaaatgaaat gtttaattta
      301 cgaaatctta ggagggcaaa aacgtggcat ttgaatttag attacccgat atcggggaag
      361 gtatccacga aggtgaaatt gtaaaatggt ttgttaaagc tggagatact attgaagaag
      421 acgatgtttt agctgaggta caaaacgata aatcagtagt agaaatccca tcaccagtat
      481 ctggtactgt agaagaagtt atggtagaag aaggtacagt agctgtagtt ggtgacgtta
      541 ttgttaaaat cgatgcacct gatgcagaag atatgcaatt taaaggtcat gatgatgatt
      601 catcatctaa agaagaacct gcgaaagagg aagcgccagc agagcaagca cctgtagcta
      661 ctcaaactga agaagtagat gaaaacagaa ctgtaaaagc aatgccttca gtacgtaaat
      721 acgcacgtga aaaaggtgtt aacattaaag cagtttctgg atctggtaaa aatggtcgta
      781 ttacaaaaga agatgtagat gcatacttaa atggtggtgc accaacagct tcaaatgaat
      841 cagctgcttc agctacaaat gaagaagttg ctgaaactcc tgcagcacct gcagcagtat
      901 cattagaagg cgacttccca gaaacaactg aaaaaatccc tgctatgcgt agagcaattg
      961 cgaaagcaat ggttaactct aagcatactg cacctcatgt aacattaatg gatgaaattg
     1021 atgttcaagc attatgggat caccgtaaga aatttaaaga aatcgcagct gaacaaggta
     1081 ctaagttaac attcttacca tatgttgtta aagcacttgt ttctgcattg aaaaaatacc
     1141 cagcacttaa tacttcattc aatgaagaag ctggtgaaat tgtgcataaa cattactgga
     1201 atatcggtat tgcggcggac actgatagag gcttattagt acctgtagtg aaacatgctg
     1261 accgtaaatc tattttccaa atttctgatg aaattaacga attagctgtt aaagcacgtg
     1321 atggtaaatt aacagccgat gaaatgaaag gtgcaacatg cacaatcagt aatatcggtt
     1381 cagctggtgg acaatggttc actccagtta ttaatcaccc agaagtagca atcttaggaa
     1441 ttggccgtat tgctcaaaaa cctatcgtta aagatggaga aattgttgca gcaccagtat
     1501 tagcattatc attaagcttt gaccacagac aaattgatgg tgcaactggc caaaatgcaa
     1561 tgaatcacat taaacgctta ttaaataatc cagaattatt attaatggag gggtaaaaca
     1621 tggtagttgg agatttccca attgaaacag atactatagt aatcggagca ggtcctggtg
     1681 gatacgttgc agcaattcgt gcagctcaat taggacaaaa agtaacaatc gttgagaaag
     1741 gtaatcttgg tggtgtttgc ttaaacgtag gatgtattcc ttcaaaagca ttactacatg
     1801 cttctcaccg ttttgttgaa gcacaacatt ctgaaaactt aggtgttatt gctgaaagtg
     1861 tttctttaaa cttccaaaaa gttcaagaat tcaaatcatc agttgttaat aaattaactg
     1921 gtggtgttga aggcttactt aaaggtaaca aagtaaacat cgttaaaggt gaagcatatt
     1981 tcgtagataa caatagctta cgtgttatgg acgaaaagag cgcacaaaca tacaacttta
     2041 aaaacgcaat cattgcaaca ggttcaagac caattgaaat tcctaatttc aaattcggta
     2101 aacgtgttat cgactcaaca ggtgctttaa atttacaaga agtaccaggt aaattagttg
     2161 tagttggtgg aggatacatt ggatcagaat taggtacagc atttgctaac tttggttcag
     2221 aagtaaccat ccttgaaggt gctaaagata tcttaggtgg cttcgaaaaa caaatgacac
     2281 aacctgttaa aaaaggtatg aaagaaaaag gtgttgaaat cgttactgaa gcaatggcta
     2341 aatcagctga agaaacagat aacggagtta aagttactta tgaagctaaa ggcgaagaga
     2401 aaacaatcga agctgattat gtattagtaa ctgtaggtcg tcgtccaaac acagacgaat
     2461 taggcctaga agaattaggt gttaaattcg ctgaccgtgg attattagaa gttgataaac
     2521 aaagccgtac gtctatcagc aatatctatg caattggtga tatcgttcca ggtttaccac
     2581 ttgctcacaa agctagctat gaagctaaag ttgctgctga agcaattgat ggtcaagctg
     2641 ctgaagttga ttacattggt atgccagcag tatgctttac tgaacctgaa ttagctacag
     2701 ttggttattc agaagcgcaa gctaaagaag aaggtttagc aattaaagct tctaaatttc
     2761 catatgcagc aaatggtcgt gcattatcat tagacgatac taacggattt gttaaactta
     2821 ttacacttaa agaagatgat actttaatcg gtgctcaagt agttggtact ggtgcatcag
     2881 atattatctc tgaattaggt ttagcaattg aagctggtat gaatgctgaa gatatcgcat
     2941 taacaatcca tgcacatcca acattaggtg agatgactat ggaagcagct gaaaaagcta
     3001 tcggataccc aatccataca atgtaataac tgattatcta taaagattca gtttttaaaa
     3061 gctgtagcat atgctacggc ttttttgttt taggtaataa agtagtgtaa ggaaattgat
     3121 ttgagatatc gttaacatgt gacatgcatg ttatactagc gatgctaaca aaagaattga
     3181 aatggagggt tcaacaatgg aatatgagta tccaattgat ttagactgga gtaatgaaga
     3241 gatgatttta gtgataaatt tctttaatca tgtagagaag tattatgaat ccggcgtgac
     3301 ggcaggagac tttatggatg catataaaag atttaaagaa attgtgcctg ctaaagcaga
     3361 ggaaaaacaa atttttaata ctttcgaaaa aagtagtggc tataatagtt acaaagcagt
     3421 tcaagatgta aaaactcact ctgaagaaca aagagtaaca gctaaaaaat aattcgttcg
     3481 aaattaacac aatttaatag gattttttct ttaaaactat tgctaataaa gctatatttt
     3541 gataccttta tcaagtgtta aacaaaatgt ttgataaaag taaacttaat atagcttttt
     3601 taggtggaaa gataaatgaa cataggtaat aaaattaaaa atcttagaag aattaaaaat
     3661 ttaacgcaag aagaacttgc tgaacgtact gacttatcga aaggctacat ttcacaaata
     3721 gaaagtgaac atgcctcacc aagtatggaa actttcttaa atattataga ggtgttaggg
     3781 acgacgccaa gtgaattttt taaagacagt gaaaatgaaa aagtattata caagaaggaa
     3841 gaacaagtta tttatgatga gtatgatgaa ggttatatat taaattggtt agtttcaaag
     3901 tcaaatgaat atgatatgga gccattaata ttaactttaa agcctggcgc atcatataaa
     3961 aattttaatc catcagagtc tgatacgttt atttattgta tgtcaggtca gataacactt
     4021 aatttaggca aagaggtata tcaagcacaa gaagaagacg ttttgtattt taaagcacga
     4081 gataatcatc gtttgtcaaa tgaatcaaac aatgaaacac gaatacttat tgtagcgaca
     4141 gcttcatatt tatagggggg atcttatttg gaaccgttat tatcattaaa atcagttagt
     4201 aaaagctatg atgatcttaa tatcttagat gacatagata ttgatattga atcaggatac
     4261 ttttatacat tattaggtcc ttcaggttgt ggtaaaacaa caattttaaa attaattgca
     4321 gggtttgaat atcctgacag tggtgaagtg atttatcaaa acaaaccaat tggtaattta
     4381 ccaccaaata aacgtaaagt gaatacagtc tttcaagatt atgcattatt tccacactta
     4441 aacgtctatg ataatatcgc tttcggtttg aaattaaaaa aattatcaaa aaccgaaatt
     4501 gatcaaaaag taactgaggc attaaaatta gttaagcttt caggttatga aaaaaggaat
     4561 attaatgaaa tgagtggcgg acaaaagcaa cgtgttgcaa ttgcacgtgc tattgtaaat
     4621 gaaccagaaa tattattgtt agatgaatct ttatccgcat tagatttgaa attgcgtact
     4681 gaaatgcaat atgaattacg agaattgcaa tctagattag gtattacatt tatatttgta
     4741 acacatgatc aagaagaagc tttagcacta agtgactttc tttttgtatt aaaagatggg
     4801 aaaattcaac aatttggtac accaacagat atatatgacg aaccagtgaa tcgatttgta
     4861 gctgatttta ttggagaatc taatattgtt gaagggcgca tggttagaga ttatgtcgtg
     4921 aatatttatg ggcaagattt cgaatgtgtc gatatgggta ttcctgaaaa taaaaaagta
     4981 gaagtcgtta ttcgaccaga agatatatca ttaatcaaag ctgaagaagg attatttaaa
     5041 gcaactgttg attctatgtt gtttagaggg gtccactatg aaatatgttg tatagacaat
     5101 aaaggttatg aatgggtaat acaaacgact aaaaaagccg aagtaggcag cgaagttggt
     5161 ctttattttg atcctgaagc cattcatatt atggttcctg gtgaaacaga agaagagttt
     5221 gataaacgta ttgaaagcta tgaggaagta gacaatgcgt aatactaata aatttctttt
     5281 aatcccgtat ttactatgga tggttatatt tattattgta tcagttgtat tactcattta
     5341 tttttcattt ttagatatca atggacattt tagtttcacg aattatcaac aaatttttac
     5401 tacaaaatat ttaaaaatgt ttgcatattc aattttatat gccgccttaa taacaattat
     5461 tacgttggct atcagttatc cagctgccta ttatattact cgctcgaaat ttcaaaatat
     5521 cttattaatg ataatgatta ttccaacatg gataaattta ttgttgaaga catatgcttt
     5581 tataggttta ttaagtcacg atggtgtgat taatcaattt ttccacttat ttaatttacc
     5641 atcattcaat ttgttattta caactggtgc ctttttagta gtggcaagtt atatttatat
     5701 accatttatg attttaccta tatttaatag catgaaagca attcctaata atttattgca
     5761 agcctcaagt gatttaggtg ctagtccatt ctatactttt agaaaagtaa tcatgccgtt
     5821 aacaaaagaa ggtgttatga ctgggattca agtaaccttt attccatcac tttcattatt
     5881 tatgattaca agattaattg caggtaataa agtcataaat ataggtacgg caatagagga
     5941 gcaattttta acaattcaaa attatggtat gggatcaact atagctatat tcttaattgt
     6001 atttatggca ttcattttaa tcattacaaa atcatctaat gggagagggt gaatcatatg
     6061 aaatggtatg gaaagctgta tatcgggata cttttagcga ttttatacat cccaatattc
     6121 tttttaatgt tctattcatt caattcggct ggtaatatga ttcactttga acattttaca
     6181 ttagagcatt atcaatcatt atttcaaaat gatcatttaa tgtcggtcat ttttaacacg
     6241 atagctgtag cacttttagc agcctcaatt tctacagtta ttggtacatt tggtgccatt
     6301 gctatttatt atttaagaaa aaaaaagttt aaagtaactt tactaacatt gaataatgtc
     6361 ttgatggtat catccgacgt tgtcataggt gcatcattct taattatgtt tacaacgatt
     6421 ggccatttta ctggtcttgg tttaggattt tggacagttc taatatctca tattgcattt
     6481 tgcataccta tagttgtgat tatcgtctta ccacaactgt atgaaatgaa taataatatg
     6541 ttaaatgctg caagagattt aggagcgact gaaccacaat tattaagcaa cattattatt
     6601 cctaatattt taccttctat tataggagga ttctttatgg ctttaactta ttcactagac
     6661 gatttcacag taagtttctt cgttactggt aatggcttta gtgtgttatc agttgaagtt
     6721 tatgctatgg cgagaaaagg aattagtatg gaaattaatg cgatttcaac attattgttt
     6781 gctgttattg tattaggaat actaggatat tatttgattc aatacgtgat aaataagaaa
     6841 aaactaatca agcgaggtgt aaaataatga aacgtttttt acaactcatt ataggtgcat
     6901 tagttgtggg tatgctttgt cttactttaa gtcattggtt taaatctaaa gaacaagtgc
     6961 atacaaatca aaaaatttac gtatacaatt ggggcgaata tattgatcca gagttaatta
     7021 agaaatttga aaaagaaact ggcattcaag tcgtttatga aactttcgat tcaaatgaag
     7081 cgatggaagc caaaattcgc aatggtggta cacattatga tgttgctttt cctagtgaat
     7141 atacagttca aaaattgaaa agagatcatt tattgttacc aatagatcat aataaagtac
     7201 ctaatattaa aaatttagat tcagattata tgaatatgtc atttgataaa ggcaataaat
     7261 attctttacc ttatttcttt ggaactgtag gtattttata taataaagaa aagtatccaa
     7321 atgaatcatt tgatagttgg aagtcattgt ataatcctaa atttaaaaac caaattttac
     7381 tagttgacgg tgctagagag attataggca tgagtttgaa taaacttggg tataatctta
     7441 atgaccgtaa ttcgcaccat ttaaaagaag cagagcgaga tttaactaaa ttagcaccac
     7501 aagtaagagg tgtcgtaggt gatgaaatta ccatgatgct tgaacaaaat gaaggtaaca
     7561 ttgcggttgt ttggagtggt gttgcagcac ctctagtgca agaaggggat aaatataact
     7621 atgttattcc taaagaagga tcgaatttat ggttcgacaa tatggtaatt ccaaaaacgg
     7681 cacaaaataa agagggtgca tataaattta tgaatttttt attagatgct aaaaataaca
     7741 agcaaaatac tgaattcgta ggctatgcaa cgccaaacaa ggctgctcga caattgttgc
     7801 ctaaagagat taaagacgac catcgtttct atccgactaa gaaagagcaa gaacgccttg
     7861 aagtttataa agatttagga cctgaagttt taagtgaata caatgaaaac tttttgaatt
     7921 tcaaaatgtc attaaaataa aatcataaat agtagttatt tactgtatat ttagttataa
     7981 taacgggata agtactataa ggagttgtaa cgaatgactg gagaacaatt tactcaaatt
     8041 aaacgtccag taagtagatt aactgaaaaa gttctaggtt ggttatgttg ggtgatgtta
     8101 ttagtgctta ctgtcattac gatgtttata gcacttgttt cattcagtaa taacacatcg
     8161 attgctaatc ttgaaaatac attaaacaat aatgcattta tccagcaatt attagctgga
     8221 aatggctata atacaacaca atttgtaata tggttacaaa atggtatatg ggctattatc
     8281 gtttacttta ttgtttgttt gttgatttca tttttagctc tcatatctat gaatataaga
     8341 atcttgtcag gtttcttatt cttaatatca gcaattgtaa cgattccttt agttttactt
     8401 atcgttactt taatcattcc gatattattc tttattattg cgatgatgct atttataaga
     8461 aaagataaag ttgaaatggt tgcgccacaa tattatgaag agtacaatgg accgatttat
     8521 gattatcgtg aacctgtgta tgagcgccca caaccgaaag atgattatta tgatgtgcct
     8581 aaatatgaaa aagaattgga taaatcaaac actgtatatg atcaagaact ggaaagagat
     8641 aaatatgatc aatttcctaa acgtgcagtt gaaagtgaat ataatcatga tgagcgcact
     8701 gaggaagaac catcagtatt atccagacag gctaaataca aacaaaaaag tactgaagaa
     8761 ctaggtattg aagatgatgg ttattatgca gaacctgaag ttgatccaaa agaattgaaa
     8821 gcacaacaaa agcgagaaaa agctgaaatt aaagctaaga aaaaagaaaa acgtaaagct
     8881 tataaccaac gaatgaaaga acgtaggaaa aatcaaccta gcgcagttag tcaacgtcga
     8941 atgaattttg aagagcgacg tcaaatttac aacaatgata tttctgaaga acgcaattca
     9001 agtgaagtta aggacaaaaa agagcaagaa taaatattga tgatacccag attagtaagt
     9061 agaggtcttt tgcttattaa tctgggtttt tatatgaggt taattatcga taacgtttaa
     9121 ttaaagtgtt taggtgtcat aattttaaat gacgatttct ctttaccata cagctaaatt
     9181 accatcaatc tgaattcaga tgtttattat aaaaattaga tgaaaaaaat gttaatatac
     9241 aagtaaatta atgtacttat atctataggc tcgtagtata cttattaaaa tattaaacaa
     9301 agaaaggatt ttaagatgaa tagaaaacct gaattaatca tggcttggat tgcaaatagt
     9361 attagtatta tttatttatt agttatgggg ctatcctatt tttctttaaa aagtggtaat
     9421 gcatcacaac gtgaagaatt agcgaagcaa ttatctcaga acggtggcaa ggtttcttta
     9481 gatatgcttc agacaacaat gggtgcatta gcaattattt tattaatttc aacactttat
     9541 ggtatatttg cgacaatttg tattaaagga cgtagaaaat tatcgattat actttttgtt
     9601 atcgcgataa ttgtatgttt gatggctctt aatttaattg caattgtctt atggattatc
     9661 gtgatgatta tgttgatttc taaaaaagaa tcaaaagaaa ctacccataa ggacgatgag
     9721 tatatttatc attaatatgt tcatagcaaa agaaaaacca ttaaaatgtt aagttgttaa
     9781 ttattagata caac
//
LOCUS       DAMZPP010000112         9399 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMZPP010000112 DAMZPP010000000
VERSION     DAMZPP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.112"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..504
                     /locus_tag="POH73_002086"
     CDS             <1..504
                     /locus_tag="POH73_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020807974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDI6888017.1"
                     /translation="LVGTVLSGFEYRAQKEKYDNLYKFFKDNEKKYQYTGFTKKAINK
                     TQNNGYENEYFYIVANIPTLQEYRKYYEPLIKKNNLNFKKGMKEARKGVGYKAEIEVH
                     TTLFSKSSNFSKDKKLDDVIDLSESTKKLHLNFENTKIFLQLAKSTISTNRVNYSDNE
                     SIRIEVE"
     gene            562..1470
                     /locus_tag="POH73_002087"
     CDS             562..1470
                     /locus_tag="POH73_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDI6888018.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSNSSLRSEDKGDDMSTLVGTVLSGFEYRAQKEKYDNLYKFFK
                     DNEKKYQYTGFTKEAINKTQNSGYENEYFYITTIPYNLTEYRDYFEPLLNKSDSEFSK
                     ELSNVKKQLKDKSKLSVTTTLFSKKKNYTKKSNSENVIKMAEEIKKEKEIPNGIDLSI
                     KFSDNKINTVKPNYNGKSTSEYGVFD"
     gene            1675..1839
                     /locus_tag="POH73_002088"
     CDS             1675..1839
                     /locus_tag="POH73_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888019.1"
                     /translation="MTILTQLSDRIKKTIPTTDKTTNQSNLPNQSTQITNQNYPNNAR
                     KSPMKHSPIT"
     regulatory      1922..2056
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2118..2663
                     /locus_tag="POH73_002089"
     CDS             2118..2663
                     /locus_tag="POH73_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDI6888020.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIIVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(2769..3017)
                     /locus_tag="POH73_002090"
     CDS             complement(2769..3017)
                     /locus_tag="POH73_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDI6888021.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            3125..4078
                     /locus_tag="POH73_002091"
     CDS             3125..4078
                     /locus_tag="POH73_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6888022.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLENITEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTM
                     QQDFRFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEFEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTRLLEQFTIEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVEQEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            4068..5447
                     /locus_tag="POH73_002092"
     CDS             4068..5447
                     /locus_tag="POH73_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDI6888023.1"
                     /translation="MLHDILRNKFGFENFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLEIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQDVLTTLHSFYSIGALKQVFKKSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            5600..7039
                     /gene="ebpS"
                     /locus_tag="POH73_002093"
     CDS             5600..7039
                     /gene="ebpS"
                     /locus_tag="POH73_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HDI6888024.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEGYYNKNAFAMDKSHPEPIEDNDKHETIKEAENNTEHSTVSDKSEA
                     EQSQQPKPYFATGANQANTSKDKHDDVTVKQDKDESKDHHSGKKGAAIGAGTAGVAGA
                     AGAMGVSKAKKHSNDAQNKSNSGKVNNSTEDKASEDKSKEHHNGKKGAAIGAGTAGLA
                     GGAASNSASAASKPHASNNASQNNDEHDHHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKDDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQQQQRQGGGQRHTVNGQENLYRIAIQYYGS
                     GSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            7177..7263
                     /locus_tag="POH73_002094"
     CDS             7177..7263
                     /locus_tag="POH73_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888025.1"
                     /translation="MAYQLNFLHSHQKDKTPQSTNLTRKNKK"
     gene            7424..8410
                     /locus_tag="POH73_002095"
     CDS             7424..8410
                     /locus_tag="POH73_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HDI6888026.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(8525..>9399)
                     /locus_tag="POH73_002096"
     CDS             complement(8525..>9399)
                     /locus_tag="POH73_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDI6888027.1"
                     /translation="LHQDVINQYAQIDELNPFNVPSPHMTIQHVKQLKDIILEAVTNK
                     YYDGFVITHGTDTLEETAFLLDLILGIEQPVVITGAMRSSNEIGSDGLYNYISAIRVA
                     SDEKARHKGVMVVFNDEIHTARNVTKTHTSNTNTFQSPNHGPLGVLTKDRVQFHHMPY
                     RQQALENVNEKLNVPLVKAYMGMPGDIFSFYSREGIDGMVIEALGQGNMPPSALEGIQ
                     QLVSLNIPIVLVSRSFNGIVSPTYAYDGGGYQLAQQGFIFSNGLNGPKARLKLLVALS
                     NNLDKAEIKSYFEL"
BASE COUNT         3559 a         1441 c         1596 g         2803 t
ORIGIN      
        1 ttagttggta cagtgttgag tggctttgaa tatcgagcac aaaaagaaaa gtatgacaat
       61 ttatataaat ttttcaaaga taatgaaaag aaatatcaat atacaggatt tacaaaaaag
      121 gcaattaaca agacacaaaa taatggctat gaaaatgaat atttttatat agttgcaaat
      181 ataccgacgc tccaagaata tagaaaatat tacgaacccc taataaagaa gaataatctg
      241 aattttaaaa aaggtatgaa agaagcaagg aaaggagtag gctataaagc tgaaatagaa
      301 gtacatacta cattgttttc gaaaagtagt aacttttcaa aggacaaaaa attagatgat
      361 gttatagatt tgtctgaaag tacgaaaaag ttacacctta attttgaaaa tacgaaaata
      421 tttttacaac tagcaaaatc tactattagt actaatcgag ttaattacag tgataatgag
      481 tctataagga ttgaggtcga atgacttgaa atcagctaat ttctctatat tctaaacaaa
      541 cacataaatg ggtgatgagc tatgtttaaa agaactaaac taatcttaat agcaacacta
      601 ctgctatcag gatgttcaac taccaataac gaatccaaca atgaaacaaa atcagtacca
      661 gaagaaatgg aagcttccaa atatgtagga caaggctttc aaccacctgc agaaaaagat
      721 gcgattgaat ttgcgaagaa gcataaagat aaaatagcta aacgtggtga acaatttttt
      781 atggataact tcggtctaaa agttaaagct accaatgttg taggtagtgg cgacggtgta
      841 gaagtattcg tgcattgtga tgaccacgac atcgtattta atgcgagtat tccatttgac
      901 aaatcaatta ttgacagtaa tagctcatta agaagtgagg acaaaggcga tgatatgagt
      961 actttagttg gtacagtgtt gagtggcttt gaatatcgag cacaaaaaga aaagtatgac
     1021 aatttatata aatttttcaa agataatgaa aagaaatatc aatatacagg atttacaaaa
     1081 gaggcaatta acaagacaca aaatagtgga tatgaaaatg aatattttta tattacgact
     1141 ataccgtata atttaactga gtatagagac tattttgaac cattattaaa caaaagtgac
     1201 agtgaatttt caaaagaatt gtcaaatgtt aagaagcaat taaaagataa gtctaaactt
     1261 tcggtaacta ctactctatt tagtaaaaaa aagaactata ctaaaaaaag taacagtgaa
     1321 aatgtaataa aaatggcaga agaaataaaa aaagaaaaag agataccaaa cggtatagat
     1381 cttagtataa aattttcgga caataaaata aatacagtta aaccaaatta taacggtaaa
     1441 agcacttcag aatatggtgt gtttgactaa gaataaaata aatgatgaat atttagcgga
     1501 gattagtgta tattggctag attaagtatt gaaagacaat tctactattg ttgtgaaggc
     1561 aaataagtag aaggttttaa gtataacttc tggcgattta aataataata tatatggaag
     1621 tgctgataca aaacttttta acctactaga ttcttataat ttgctttcca ttttatgacg
     1681 attttgaccc aattgagtga tagaatcaaa aaaaccatcc caacaacaga caaaaccaca
     1741 aaccaatcaa acctacccaa tcaaagtacg caaatcacca atcaaaacta cccaaacaat
     1801 gctcgcaaat caccaatgaa acattctcca attacgtaac tatgatctaa ttaaagcata
     1861 gttattgagg ttttgtgata tatagtataa aattaatgag aattaaattt aataatgtaa
     1921 aattcatctt cggggtcggg tgtaattccc aaccggcagt aaataaagcc tgcgacctgc
     1981 tagtatgtat catattagtg gctgatctag tgagattcta gagccgacag tatagtctgg
     2041 atgggagaag atggaggttt tttgttgtgc aataatcctc ctattcttac gagatgaatg
     2101 gaaggagaaa attgaatatg caacaaaata aacgtcttat cacaataagt atgttgagcg
     2161 cgattgcgtt tgtattaact tttatcaagt ttcctatacc atttttgcca ccatacttaa
     2221 ctttagattt tagtgatgta ccgtcactac tagctacatt tacgtttgga ccagttgccg
     2281 gtatcatagt tgcactggtt aaaaatttat tgaactactt atttagtatg ggcgatccag
     2341 ttggaccatt tgctaacttt ttagcaggcg caagtttctt attaactgct tacgccatct
     2401 ataaaaataa acgttcaaca aaatctttga ttactggatt aatcattgca acaatcgtta
     2461 tgactattgt gttgagtatc ttgaactatt tcgttctatt acctttatac ggtatgatat
     2521 ttaacttagc tgatatcgca aataatctta aagtaatcat tgtttcagga attataccat
     2581 tcaatattat taaaggtatc gtcatttcta ttgtatttat tttactatat agaaggcttg
     2641 cgaatttctt gaaaagaatt taatcaaatt aaagcaaaat aatatacaca taataataaa
     2701 aagcaggtga ctatcaataa acgatagctt gcctgctttt tctatgaaac atttgtctaa
     2761 aaaatcaatt attcaaattt taaagcgtcc ccatcaaatg attcgtctgc aattttaata
     2821 gaatctgtag ggcatccatc aattgcatct tccatatctt catataattc ctcaggtact
     2881 tctgcggtac cttggttatc gtcaaggatt acgaaagcaa taccttcgtc gtcgtaatca
     2941 tatatatctg gcgctgctgc accgcatgca ccacatgcaa tacaagtatc catatcaacg
     3001 attgtatatt ttgccaatgt cttcgcctcc tttgataaaa atgctaaaat agtaatgtga
     3061 ctaaaatttt agacagcatc atttttattt tcaaattatc cgttttacag agtgagggtt
     3121 aaacttgcaa cacattataa aaacagcatt acaacaaaca tttaactata aaacaaataa
     3181 aagtatttac aatatcttag ttggtaagaa atctcaccaa accttttttg acgcttgtag
     3241 tcaacaacag ttgtcattat atcacagttt accactatta aaatatccgt cttttgagct
     3301 atttttagaa aatataactg aatttaatgc tgaaatggaa atcatgttgc atcctagata
     3361 tacatttgaa agtatggggc aaacatttca agcaattcaa ctattagtgc aaaccatgtc
     3421 taatactatg cagcaagatt ttcgtttcgt accaatctct caaaataata agatacaaga
     3481 aactgtaaaa attgtctata actatattaa agaaaaccaa ttacaaattg actttgaaaa
     3541 tgaactacat aacttgttta aagcaataac tttaaaaggg ccatgttact tacattatta
     3601 tttgcaaggc tatgatgaac caatgtatac gagacagcaa gttagtttaa tagaaaaact
     3661 atctcaacag caattgtttg aattcgaaat gaataattta gtgacaatga tgtttgaatt
     3721 agaaagtgga gaatatacta ttttatcaaa aataataatg aaacctacat tattaaatca
     3781 aacctatatt acttatacaa gattgcttga acaattcacg atagaagata tagcggctca
     3841 acaacaagtt aaaatcaata ctatcgaaga tcatgtactt gaaatcttaa tcaaaggtta
     3901 tatgtctaat tacgatgatt atgttgaaca agaagatcaa ctgcaatttt tgaattttta
     3961 tcaacaacat cgtggtgaac gattaaaatt ttacaaagaa caatttgaca cgttatcata
     4021 ttttcaatta aaagtattaa tcgttggttt tgaaagaggt gatctgaatg ttgcatgata
     4081 ttttacgaaa caaatttggc ttcgagaatt ttaaaccggg acaacaggaa attatagaaa
     4141 gtataatgtc tcaacaacac actctaggta ttcttccaac tggaagtgga aagagtttgt
     4201 gttatcaaat acctacgtat ttatcaggta agccgacatt aattatctca ccgttaatat
     4261 ctttaatgga tgaccaagtt atgcagttga aaataaatgg agaaaaacgt gtaacatgta
     4321 ttcactctgg tatggatgaa attgagaaaa agcataatat taaatgttta cgacatagcc
     4381 gcttcatctt tctaagtcca gaatttctcc tgcagccgtc aaattttaaa ttaatatcta
     4441 tgatagactt tggcatgatt gttctagatg aagcacattg tctatctgaa tggggatatg
     4501 atttcagacc acattatgct ctaattggaa aagtaacaaa gcattttaaa gaagcggttg
     4561 tcttagcatt gacagcaact gcaccaccgc atttgcaaga tgatttgacg gaaatgctag
     4621 agattcaatt caacgttatt aaaactacaa tgaatcgccc aaacataagc tttaagcatc
     4681 ttaattttca tgatgatgaa gataaaattg agtggttgct gccgtttcta caacagtcgg
     4741 gaccaacgat tatttatgtc tcatcgaaaa agatgtgtct gaatttagcg caacttattt
     4801 atgattcagg ttttcttact ggcatttatc atggtgatat gaattatcaa gagcgacaca
     4861 cagttcaaca acaattttta aataatgata ttccgattat agtcgcaacg agtgcttttg
     4921 gtatgggaat taataaaaaa gatattcgca caatcattca ctttcatctt tcaacaagtc
     4981 cttctaacta cattcaagaa attggacgtg cgggtcgcga tggtgaacta agtcaggcaa
     5041 ttagtttatt tcaaccggac gataaatata ttttagaaac gttattattt gcagatatga
     5101 taacagaaga agatgtacaa aatttcgaaa taggagaatt tttagcaccc gataaacaag
     5161 acgttttgac aacgttgcat tcattctata gtatcggcgc cttgaaacag gtatttaaga
     5221 aatcatttaa acgaaagcaa ttaggattct ttcgtatgat tggctattgc aaattggatc
     5281 aatgtagacg gaagtattta ttagaatttt tcggtgaata tccaccggca caagatcgat
     5341 gttgtgacaa tgattctaat ataactgata tcgcaatttt aaataagaag aaggtaatta
     5401 gaagtattgg atttgatgaa aagttgcaaa atttatttct cagatagtat tactttacta
     5461 aaagaaaatt gacaagctat aattagtgta tacacaattg aaaaatgatt gaaataattt
     5521 tgaaaaatat acataaacat atgtcatgtg ggtatatttt atgtaaaatc attgtaatag
     5581 aatagaaagg aagatggcta tgtctaataa ttttaaagat gactttgaaa aaaatcgtca
     5641 atcgatagac acaaattcac atcaagacca tacggaagat gttgaaaaag accaatcaga
     5701 attagaacat caggatacaa tagagaatac ggagcaacag tttccgccaa gaaatgccca
     5761 aagaagaaaa agacgccgtg atttagcaac gaatcataat aaacaagttc acaatgaatc
     5821 acaaacatct gaagacaatg ttcaaaatga ggctggcaca atagatgatc gtcaagtcga
     5881 atcatcacac agtactgaaa gtcaagaacc tagccatcaa gacagtacac ctcaacatga
     5941 agagggatat tataataaga atgcttttgc aatggataaa tcacatccag aaccaatcga
     6001 agacaatgat aaacacgaga ctattaaaga agcagaaaat aacactgagc attcaacagt
     6061 ttctgataag agtgaagctg aacaatctca gcaacctaaa ccatattttg caacaggtgc
     6121 taaccaagca aatacatcaa aagataaaca tgatgatgta actgttaagc aagacaaaga
     6181 tgaatctaaa gatcatcata gtggtaaaaa aggcgcagca attggtgctg gaacagcggg
     6241 tgttgcaggt gcagctggtg caatgggtgt ttctaaagct aagaaacatt caaatgacgc
     6301 tcaaaacaaa agtaattctg gcaaggtgaa taactcgact gaggataaag cgtctgaaga
     6361 caagtcaaaa gaacatcata atggtaaaaa aggtgcagca atcggtgctg gaacagcagg
     6421 tttggctgga ggcgcagcaa gtaatagtgc ttctgccgct tcaaaaccac atgcctctaa
     6481 taatgcaagt caaaacaatg atgaacatga ccatcatgac agagataaag aacgtaaaaa
     6541 aggtggcatg gccaaagtat tgttaccatt aattgcagct gtactaatta tcggtgcatt
     6601 agcgatattt ggaggcatgg cattaaacaa tcataataat ggtacaaaag aaaataaaat
     6661 cgcgaataca aataaaaata atgctgatga aagtaaagat aaagacacat ctaaagacgc
     6721 ttctaaagat aaatcaaaat ctacagacag tgataaatca aaagatgatc aagacaaagc
     6781 gactaaagat gaatctgata atgatcaaaa caacgctaat caagcgaaca atcaagcaca
     6841 aaataatcaa aatcaacaac agcaacaaca acgtcaaggt ggtggccaaa gacatacagt
     6901 gaatggtcaa gaaaacttat accgtatcgc aattcaatac tacggttcag gttcaccgga
     6961 aaatgttgaa aaaattagac gtgccaatgg tttaagtggt aacaatatta gaaacggtca
     7021 acaaatcgtt attccataat ataactatat aaattgtaac tgaactgcta tatacgaacg
     7081 tgcatattat aaagtttctc taaatattat tgaaaactta aattgcgctt ataagtatgt
     7141 agcagttttt ttatttaata agtatggaca tgaatattgg catatcaatt aaatttttta
     7201 catagtcatc aaaaagataa aacaccacaa tcaacaaatt taacgaggaa gaataaaaaa
     7261 taaatcaaca tattaaattg tagtgttatt caactccgta gctaacaatt ctctatgcat
     7321 attaaacaaa ttgtcgaaaa tatatcataa atcttcaagc acagacttag cgcatcaatc
     7381 actgaactgt tataatagtt tgggattaaa ggaggccgga acaatgcaaa aagttgaaag
     7441 tatcataatt ggtggagggc catgcggatt aagtgcggct attgaacaaa aaagaaaagg
     7501 tattgatacc ttaattattg aaaagggtaa tgtcgttgaa tcaatctaca attatcctac
     7561 tcaccaaaca tttttctcat caagtgataa attaagtatt ggggacgtac cgtttattgt
     7621 tgaagaaagt aaaccaagac gtaatcaagc gctagtttat taccgagaag ttgtaaaaca
     7681 tcatcaatta aaagtaaatg catttgaaga agtattaact gttaaaaaaa tgaataataa
     7741 atttactatt actacgacga aagatgttta tgaatgtcga tttttaacaa tcgcgacagg
     7801 ctattatggt cagcataata cattagaagt tgaaggtgcg gatttaccta aagtgttcca
     7861 ttattttaaa gaggcacatc cgtattttga tcaagatgtt gttattatcg gtggtaagaa
     7921 ttcggctatc gatgctgctt tggagttgga aaaagctggt gctaacgtga cggttctata
     7981 tcgtggtgga gattattcgc cttcaattaa accgtggata cttccaaatt tcacagcatt
     8041 agtaaatcat gaaaaaattg acatggaatt taatgctaat gttacccaaa taactgaaga
     8101 tactgtgact tatgaagtaa atggtgaaag taaaacgata cacaatgatt atgtatttgc
     8161 gatgattggt tatcatcccg attatgaatt tttaaaatct gtaggcattc aaattaatac
     8221 aaatgaattt ggaacagcgc ctatgtataa taaagaaaca tacgaaacaa atatcgaaaa
     8281 ttgctacatt gcaggtgtga ttgctgcagg gaacgatgcg aataccattt ttattgaaaa
     8341 tggtaaattc cacggtggta ttattgctca aagcatgcta gctaagaaac aaacgccgtt
     8401 agaatcataa aaataaaggt ctatgtacta aataacttag ttttacaacg actgacattc
     8461 atgatatgtc aaatgaggtt gatgactaat tattgtacat agaccttttt atgttacgta
     8521 atcattataa ttcaaaatat gatttgattt cagctttatc taaattattg cttaacgcga
     8581 ctaataattt taatcttgct tttggaccat tcaaaccgtt agaaaaaata aaaccttgtt
     8641 gtgcgagttg gtaaccacca ccatcgtatg cgtaagttgg actcacaata ccattaaagg
     8701 aacgtgaaac tagcacaata ggtatattta aagatactaa ttgttgaatg ccttctaatg
     8761 cgcttggagg catgttgcct tgccctaacg cttcaataac cataccatcg ataccttcac
     8821 gactataaaa actaaaaatg tcacctggca tacccatata tgcttttact aatggtacat
     8881 ttagtttctc attgacattt tccaatgctt gttggcgata tggcatatga tggaattgca
     8941 cacgatcctt tgtcaataca cctagcggac catgatttgg actttgaaat gtgtttgtat
     9001 tagacgtatg tgttttggta acattacgcg ccgtatgtat ttcatcatta aatacaacca
     9061 tcacgccttt atgtcgggcc ttttcatcag aggcaacgcg aatagcggaa atataattat
     9121 ataatccgtc agaaccaatt tcattagacg agcgcattgc gccagtaata acaacaggtt
     9181 gctcgatacc taatattaaa tcaagtaaaa aggcagtttc ttctaacgta tcggtaccat
     9241 gcgtgataac gaaaccatca taatatttat ttgttactgc ttctaaaata atatccttta
     9301 attgtttaac atgttggatt gtcatatgag gtgatggtac attaaaagga tttaattcat
     9361 ctatttgtgc atattgattt atgacatctt gatgcagtg
//
LOCUS       DAMZPP010000113         9160 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMZPP010000113 DAMZPP010000000
VERSION     DAMZPP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9160
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.113"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..143)
                     /locus_tag="POH73_002097"
     CDS             complement(<1..143)
                     /locus_tag="POH73_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDI6888028.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENN"
     gene            complement(470..1336)
                     /locus_tag="POH73_002098"
     CDS             complement(470..1336)
                     /locus_tag="POH73_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDI6888029.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            complement(1836..1940)
                     /locus_tag="POH73_002099"
     CDS             complement(1836..1940)
                     /locus_tag="POH73_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888030.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKKVLYGEMQ"
     gene            2035..3348
                     /locus_tag="POH73_002100"
     CDS             2035..3348
                     /locus_tag="POH73_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein sbi"
                     /protein_id="HDI6888031.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVQKHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKAESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNKYYQYKGLIDKTVLTTIGSGYGSYIKPLEVSKESGNLAKSYAQVRNYVT
                     ESINTGKVLYAFYQKPELVKTAIKAQETATTFKNALTGIFKSFWK"
     gene            3884..4813
                     /gene="hlgA"
                     /locus_tag="POH73_002101"
     CDS             3884..4813
                     /gene="hlgA"
                     /locus_tag="POH73_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HDI6888032.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHEKGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
     gene            5363..6310
                     /gene="hlgC"
                     /locus_tag="POH73_002102"
     CDS             5363..6310
                     /gene="hlgC"
                     /locus_tag="POH73_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HDI6888033.1"
                     /translation="MLKNKILATTLSVSLLAPLANPLLENAKAANDTEDIGKGNDVEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKNTNHIKS
                     MRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFKNRNYTVKYEVNWKTHEIKVKGQN"
     gene            6312..7288
                     /gene="hlgB"
                     /locus_tag="POH73_002103"
                     /pseudo
     CDS             6312..7288
                     /gene="hlgB"
                     /locus_tag="POH73_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
     gene            complement(7340..7807)
                     /locus_tag="POH73_002104"
     CDS             complement(7340..7807)
                     /locus_tag="POH73_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDI6888034.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLILSVILNLPVWVVLLNAIPGVIFTLIVAIPLYLILRKRMAVLLR"
     gene            complement(7818..8510)
                     /locus_tag="POH73_002105"
     CDS             complement(7818..8510)
                     /locus_tag="POH73_002105"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HDI6888035.1"
                     /translation="MYSIKMRSSNQDIHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIKQIKALQIIEDDKANLQHLTQKCGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGHRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFASAKIGYHRLFDIKPVNTRYGGRIIFVDDRIDLNHYILFL
                     ESTPKQVVYETV"
     gene            complement(8521..>9160)
                     /locus_tag="POH73_002106"
     CDS             complement(8521..>9160)
                     /locus_tag="POH73_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HDI6888036.1"
                     /translation="EYPNQNIQKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDV
                     SHSFGIENYSNYQGIDILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPI
                     YNLYFIKRNIEKLINADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEA
                     AENIHQTLLKHHVFTSYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
BASE COUNT         3461 a         1575 c         1285 g         2839 t
ORIGIN      
        1 atgttatttt ctcttacttt ttcacctgct ctagtcgctt cattaatacc ttgttcagat
       61 aaattaacat cttcccatcc agtaaataag tttttagcat tccactcgct ttgtccatga
      121 cgacataaaa ttaattttgg cataacttat aacctctttc ctttaatctt taggacgtta
      181 tttcagggat attttaagat gatttaatct tagcaatttt cttttcaata tgttttaaaa
      241 ttcaatgatt aaatcaatta tcactagaca ttaaatacat aaatcctatt ccccattttc
      301 attttttaat tcataaatga atcaataacc acctaataac aaatcatatt atacaccttt
      361 gtccgtcatt tttctaaggt ttaaaaaata tttttaggtg cgcctaaaaa taaatgtaat
      421 aaaaacgcct cctcagatat ttatatatct atgaagacgt ttaaatacat tatagatggt
      481 ctggttctgg gtgaacgtat actgaggaaa tacctttttt gtgcaaatga tgttcgacat
      541 tatcacaaat ttgatgcgct tctactaagg aaaggttagc atctacaaca attgtgacat
      601 caataaacac actacttcca tggtaacgcc ctttaatact tttaacttct tgtacttcat
      661 caacttctaa aatatcattg cgatacgctt ctaattcagt ttcattgaaa ccatcactca
      721 acataaaaat tgcttcttta aaaataccaa aaccagtata aacgattagt aagcctagca
      781 atgttgctaa aataatatcg acaattggga aaccgatttg cgtaaaaatt aatcctatcg
      841 ccgttccaat gctgactaaa ctatctgata aattatcttt ggcagcagaa tttaaagaac
      901 tacttttcgt tcttttcgct agtctttgat tgactgcaaa tacaatcaac attacaagac
      961 cactgattaa gctgacgata attgttattg cgttaggtac aacgtcatct tctttgaaca
     1021 aacgaggtgc attttgaata actacttgga tacctacaaa cataatgaca aatgacacca
     1081 ataatgaaga aatattttca gacttcaaat ggccgtaagg atgatttcga tcggcaggtt
     1141 taattgaaat tttcaatcca ataataacag ctaaagaaac gataatatct gtcatattgt
     1201 ttaatgcgtc agctcttaca gctgcagagt taaagacaaa acccgtgaca tacttaacaa
     1261 tagataagat tatatataca atcaaactca aataagcacc gcgttgcgcc aatttaagat
     1321 tttcattatg agacatgcgt tgaaccacct tgaattagta tagtaacaat attatgaatg
     1381 atttatttta aatttacaac gtttttaatt tttattaaat ttttataaaa ttaaactaat
     1441 ttattcattc gcaaccccta aaaatatttt ttagcctttc tgcgaatttt atgagctaga
     1501 aaggcttatg cattagtagc gaagctatct aatgcgttcc tttattttct gcggatttta
     1561 tgagctagaa aggcttatgc agttgacttt ttacgtccaa ctgcgttcct ccgtcttctt
     1621 caaatttatt tgttagaaag gcgcccaact ctccctgttt gttaactttc gtctcgaaag
     1681 tttctatgtt gggaccctat gcatgagttg cgaagctatc taatgtcttg acctaattat
     1741 atagaagaaa aagtgcatca gtgacaaatc aaatgagatt tctactctac caaactctct
     1801 tcgaaagaca attttctcct ctatttatta gcaaactatt gcatttctcc atatagtact
     1861 ttcttactta aaatacgctg aatgttctga attaaacgct tttttacata gttaacacta
     1921 gttaatctat tagttaacat tagttaataa ttagttaatt tccatttgta ttctcatgtg
     1981 ataaattcta aaagcataca ataaatttaa tatgtaaaaa gaaagggaat acacatgaaa
     2041 aataaatata tctcgaagtt gctagttggg gcagcaacaa ttactttagc tacaatgatt
     2101 tcaaatgggg aagcaaaagc gagtgaaaac acgcaacaaa cttcaactaa gcaccaaaca
     2161 actcaaaaca actacgtaac agatcaacaa aaagcttttt atcaagtatt acatctaaaa
     2221 ggtatcacag aagaacaacg taaccaatac atcaaaacat tacgcgaaca cccagaacgt
     2281 gcacaagaag tattctctga atcacttaaa gacagcaaga acccagaccg acgtgttgca
     2341 caacaaaacg ctttttacaa tgttcttaaa aatgataact taactgaaca agaaaaaaat
     2401 aattacattg cacaaattaa agaaaaccct gatagaagcc aacaagtttg ggtagaatca
     2461 gtacaatctt ctaaagctaa agaacgtcaa aatattgaaa atgcggataa agcaattaaa
     2521 gatttccaag ataacaaagc accacacgat aaatcagcag catatgaagc taactcaaaa
     2581 ttacctaaag atttacgtga taaaaataac cgctttgtag aaaaagtttc aattgaaaaa
     2641 gcaatcgttc gtcatgatga gcgtgtgaaa tcagcaaatg atgcaatctc aaaattaaat
     2701 gaaaaagatt caattgaaaa cagacgttta gcacaacgtg aagttaataa agcacctatg
     2761 gatgtacaaa agcatttaca gaaacaatta gacgcattag tagctcaaaa agatgctgaa
     2821 aagaaagtgg cgccaaaagt tgaggctcct caaattcaat caccacaaat tgaaaaacct
     2881 aaagcagaat caccaaaagt tgaagtccct caaattcaat caccaaaagt tgaggttcct
     2941 caatctaaat tattaggtta ctaccaatca ttaaaagatt catttaacta tggttacaag
     3001 tatttaacag atacttataa aagctataaa gaaaaatatg atacagcaaa gtactactat
     3061 aataaatatt accaatataa aggtttgatt gataaaacag ttttaacaac tatcggtagt
     3121 ggctatggtt catacattaa acctcttgaa gtaagcaaag aaagcgggaa cttagctaaa
     3181 tcatatgcac aagtaagaaa ctatgtaact gagtcaatca acactggtaa agtgttatac
     3241 gcattctacc aaaaaccaga attagtaaaa acagctatta aagctcaaga aacagcaaca
     3301 actttcaaaa acgctttaac aggcatattc aaatcattct ggaaataatc aatcaaaaat
     3361 atcttctcta gttttacatc attatttaaa taattttcgt aaaaaaccgt gattaaaaag
     3421 aaccgttgat tctcaatcga atctgcggtt cttttttcat tttccatcaa ttaaatgttt
     3481 cttcgctatt tgtcagctca cttttttacc tgcaacttgt taaataatcc ttacatcgtt
     3541 aacgaatagt tcatcattta gttgaatcag ctcaacttta ttaacttcaa attttcacaa
     3601 actattgcac aatccattcc tctaccactt caagcacctt aattaaacat taattcaata
     3661 gaataagact tgcaaaacat agttatgtag ctatataaac gcctgcgacc aataaatctt
     3721 ttaaacataa cataatgcta aaacatcatt taacaatgct aaaaatgtct cttcaataca
     3781 tgttgatagt aattaacttt taacgaatag ttaattcgaa aacgcttaca aatagattat
     3841 tatatatatg aacttaaaat taaatagaaa gaaagtgatt tctatgatta aaaataaaat
     3901 attaacagca actttagcag ttggtttaat cgccccttta gccaatccat ttatagaaat
     3961 ttctaaagca gaaaataaga tagaagatat cggccaaggt gcagaaatca tcaaaagaac
     4021 acaagacatt actagcaaac gattagctat aactcaaaac attcaatttg attttgtaaa
     4081 agataaaaaa tataacaaag atgccctagt tgttaagatg caaggcttca tcagctctag
     4141 aacaacatat tcagacttaa aaaaatatcc atatattaaa agaatgatat ggccatttca
     4201 atataatatc agtttgaaaa cgaaagactc taatgttgat ttaatcaatt atcttcctaa
     4261 aaataaaatt gattcagcag atgttagtca gaaattaggc tataatatcg gcggaaactt
     4321 ccaatcagcg ccatcaatcg gaggcagtgg ctcattcaac tactctaaaa caattagtta
     4381 taatcaaaaa aactatgtta ctgaagtaga aagtcagaac tctaaaggtg ttaaatgggg
     4441 agtgaaagca aattcattcg ttacaccgaa tggtcaagta tctgcatatg atcaatactt
     4501 atttgcacaa gacccaactg gtccagcagc aagagactat ttcgtcccag ataatcaact
     4561 acctccttta attcaaagtg gctttaatcc atcatttatt acaacattgt cacacgaaaa
     4621 aggtaaaggt gataaaagcg agtttgaaat cacttacggc agaaacatgg atgctacata
     4681 tgcatacgtg acaagacatc gtttagccgt tgatagaaaa catgatgctt ttaaaaaccg
     4741 aaacgttaca gttaaatatg aagtgaactg gaaaacacat gaagtaaaaa ttaaaagcat
     4801 cacacctaag taaacagttc aatcatttta taaaatcctg ggacacttca tacttgtctc
     4861 aggatttttt aacatattga atcagcctca taacattaaa ttattttatc gtacaactga
     4921 tttttataag agcaaagtat cgcatcatag tagatacaca aataataaaa atgaaacaat
     4981 ttaacttgaa agcttaaata aatattctca agttaataaa taattaactt ttagatggat
     5041 tcatcaaaaa tcgcaaaaaa gaacaatttg tattttacaa acattaatta aaaataaaag
     5101 caagacattc gtgcaatcgg ttgccttaaa atgtttacaa ctgtcaacaa taccaaggtt
     5161 ttattaacta tatttctcac aaaattagct tttagcattc caaacaaaaa aggttaaatt
     5221 gaacggaatt atggcatttt taacttaatt gtaaaaaaag ttgataatgg tcaattgtta
     5281 atgaacagtt aattataata acgtccaaaa tatattatta tttaattaag ttaaataaaa
     5341 ttatagaaag aaagtgaaaa ttatgcttaa aaataaaata ttagctacaa ctttatctgt
     5401 aagcttactt gcccctcttg ccaatccgtt attagaaaat gctaaagctg ccaatgatac
     5461 tgaagacatc ggtaaaggaa acgatgtaga aattatcaaa aggacagaag ataaaacaag
     5521 taataaatgg ggcgtgactc aaaatatcca attcgacttt gtgaaggata aaaaatataa
     5581 caaagatgct ttgatcttaa agatgcaagg attcattagc tctcgaacaa catattataa
     5641 ctataaaaat acaaatcata ttaaatctat gcgttggcca ttccaatata atattggttt
     5701 gaaaacaaat gataaatatg tttctttaat caattattta ccgaaaaata aaattgaatc
     5761 tacaaacgta agtcagacat taggatacaa tattggtggt aatttccaat cagccccatc
     5821 acttggtggt aatggatcat ttaactattc taaatcgatt agctatacac aacaaaatta
     5881 tgtaagtgaa gtagaacaac aaaactcaaa aagtgtttta tggggcgtca aagcgaattc
     5941 attcgccact gaatcaggtc aaaaatcagc atttgatagc gatttatttg taggctacaa
     6001 acctcatagt aaagatccta gagattattt cgttccagac agcgagttac cacctcttgt
     6061 acaaagtgga tttaaccctt catttatcgc aacagtatct cacgaaaaag gttcaagcga
     6121 cacgagcgaa tttgaaatca cttatggaag aaatatggat gtcactcatg ccattaaaag
     6181 atcaacacat tatggcaaca gttatttaga tggtcacaga gtccataatg catttaaaaa
     6241 tagaaactac actgtgaaat atgaagtcaa ttggaagact cacgaaatca aagtgaaagg
     6301 acagaattga tatgaacatg aataaattag tcaaatcatc cgttgctacg tctatggcat
     6361 tattattact ttctaatact gcaaatgctg aaggcaaaat cacaccagtt agcgtcaaaa
     6421 aagtagatga taaagttact ttatacaaaa ctacagctac agcagattca gataaattca
     6481 aaatttcaca gattttaaca ttcaatttca tcaaagataa aagttatgat aaagatactt
     6541 tagtgcttaa agctgcaggt aatattaact caggttatga aagaccaaat cctaaagact
     6601 acgacttttc aaaaatatat tggggtgcaa aatacaatgt gtctatcagt tcacaatcta
     6661 atgattcggt taatgtagta gactatgcac ctaaaaatca aaatgaagaa tttcaagttc
     6721 aaaacacttt aggctataca tttggtgtga catcagtatc tctaatggtt tatctggcgg
     6781 acttaacgga aacactgctt tttctgaaac aattaattat aaacaagaaa gttacagaac
     6841 aacattaagt cgcaacacaa attataaaaa tgtcggttgg ggtgtagaag cacataaaat
     6901 tatgaataat ggttggggac cttatggacg agatagcttc cacccaacat atggtaatga
     6961 actcttctta gctggcagac aaagcagtgc atacgctggc caaaacttca tagcgcaaca
     7021 ccaaatgcca ttattatcta gaagtaactt caatccagaa tttttaagcg tactatcaca
     7081 cagacaagat ggcgctaaaa aatctaaaat tacagtaact tatcaacgtg aaatggattt
     7141 ataccaaatt cgttggaatg gcttctactg ggcaggcgcg aattataaaa actttaaaac
     7201 tagaacattt aaatcaacat atgaaattga ttgggaaaat cacaaagtga aattgttaga
     7261 tacaaaagaa actgaaaaca ataaataact agtaaaacac ggtcgccaac agtaattgtg
     7321 acgaccgtgt tttgatttat tatcttagta agactgccat tctttttctc aatatgagat
     7381 ataaaggaat agctacaatt aaagtgaata ttacgcctgg aatcgcgttt aataacacga
     7441 cccacacagg taaatttaaa atgaccgata gtatcaatct tgataccaaa ctgcctaata
     7501 cacttgctaa aactaatgat agtacattta ttttcaataa ataaacaact gcaatagcta
     7561 taactctaaa tataatagaa ataatcacat taatcggatt aaatacgcca aatactagta
     7621 ataataggct agataataat ccacctaaaa agtacttttt aattccaaag aaagctaata
     7681 tcaataatgc tgctggtgca gataattgaa aatctaatcc tggtataatg gatggtattt
     7741 tcaaaactgc caaaatggtt aaaatcgcag caatgacact aatttgagta atatcttttg
     7801 atgtcatact aaaaccccta taccgtttca taaacaactt gcttcggtgt gctttctaaa
     7861 aataatatgt aatgatttaa atcaatacga tcgtccacaa atattattct gcctccatat
     7921 ctcgtattaa ctggtttaat atcaaataat cgatggtaac caattttagc agaggcaaaa
     7981 taacctgtcg tatacgttaa gtcatcggac acgcaaagtt ctcctttgac atacggatgc
     8041 gcattgatac aactagcaat tgctaaggca tcagtcactc tttcattaag atcaccttta
     8101 ttatttatat cttcaaacga aaaatgtgtt gccctaatcc ccctatgtcc aaatgaatct
     8161 aaacgtttac cagatatagc agatagaata atagctcctg tataaaccgt ttcattttta
     8221 atatatgtca tcccttgatt tagcgcttgt tcagtgacac cacatttttg tgttaaatgt
     8281 tggagattcg ctttatcatc ctcaataatt tgtaatgctt ttatttgttt aatcggttcc
     8341 ataatttttt gtatttttat attgagaaaa tccggttgcc cattttcatg aaaaaaacct
     8401 ttattataaa acctttgtac cgtctgttct attttgtcaa actcacaaat cgtttcagca
     8461 ccactaatat gaatatcttg attgctagaa cgcattttta tactatacat gacgatcacc
     8521 tcaatcttct tgatgcaaaa tttcaaacaa cctatctata tcttgttcag tatgaaaata
     8581 cgataatgat attcttaaca ttggcttagt cacagttgga taccttaaat aacttgtaaa
     8641 cacatgatgc tttaataatg tttgatgaat gttctcagcc gcttctatgt catcaaactc
     8701 aataaactta atcggcgagt ttgaactatt ataattaaca ttgagtgctt ttaacttttg
     8761 gttaaaatat ttactcaagc tatttaattt agtgcgtcta tcatcagcat ttattaactt
     8821 ttcaatattt ctttttataa aatacaaatt ataaattggc aaactacttg agtagatgag
     8881 tggtctaccg tgattaatta acatatcctt cacatcattt gaacttaaaa tcacaccccc
     8941 gtatgcacca catgctttag ataaactaga agtgagtata tctatgcctt gataattcga
     9001 gtaattctct attccaaaac tatgtgaaac atcgagtatc agtgttgcgt tatatttatg
     9061 ctttaatgag actaattgac caatatccac aacgtcgcca ttcgttgaaa atacactatc
     9121 agatatgatt attttttgta tattttgatt agggtattcc
//
LOCUS       DAMZPP010000114         9002 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMZPP010000114 DAMZPP010000000
VERSION     DAMZPP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9002
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.114"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..319
                     /locus_tag="POH73_002107"
     CDS             <1..319
                     /locus_tag="POH73_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HDI6888037.1"
                     /translation="YFTYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLN
                     GFGIYIGRFLRLHSVYFFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVR
                     LNK"
     gene            316..600
                     /locus_tag="POH73_002108"
     CDS             316..600
                     /locus_tag="POH73_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888038.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(755..2128)
                     /locus_tag="POH73_002109"
     CDS             complement(755..2128)
                     /locus_tag="POH73_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDI6888039.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVIAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYKVFTSFSKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKSKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DPKALYIKTYLPIFNQIDAKYLHDTQRNESNLFELGIELGRHYPRQMVDHQEKRTQVL
                     AAFKALD"
     gene            complement(2214..3767)
                     /locus_tag="POH73_002110"
     CDS             complement(2214..3767)
                     /locus_tag="POH73_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDI6888040.1"
                     /translation="MSEVKKVVKHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAVLAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSIALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSAREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKTGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
     gene            complement(4050..4337)
                     /locus_tag="POH73_002111"
     CDS             complement(4050..4337)
                     /locus_tag="POH73_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888041.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYVIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFIKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
     gene            complement(4372..5280)
                     /locus_tag="POH73_002112"
     CDS             complement(4372..5280)
                     /locus_tag="POH73_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDI6888042.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVIQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIGQDIP"
     gene            complement(5383..6309)
                     /locus_tag="POH73_002113"
     CDS             complement(5383..6309)
                     /locus_tag="POH73_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDI6888043.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIM
                     NEFGTVDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGI
                     AHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMII
                     INKIDLVTDDSIEKIDKQLSLICASIPTYKTTYGQVSLEELDLPGKDREISSHHHHHH
                     GIKSMTYTFTGPIDRQLFYQFIMKLSESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYG
                     IIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
     gene            6536..6979
                     /gene="mgrA"
                     /locus_tag="POH73_002114"
     CDS             6536..6979
                     /gene="mgrA"
                     /locus_tag="POH73_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HDI6888044.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
     gene            complement(7106..8779)
                     /locus_tag="POH73_002115"
     CDS             complement(7106..8779)
                     /locus_tag="POH73_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HDI6888045.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRNNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGNKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSIRFEN
                     MDMFEIDDKDKFEALNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAIARMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(8776..>9002)
                     /locus_tag="POH73_002116"
     CDS             complement(8776..>9002)
                     /locus_tag="POH73_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDI6888046.1"
                     /translation="LVIFDEPATGLDIQTEHMIQNVLFQHFKGTTMIVIAHRDNTIRH
                     LQRRLYIENGRLIADDRNISVNITENGDDL"
BASE COUNT         3069 a         1614 c         1278 g         3041 t
ORIGIN      
        1 atactttacc tatttactgc ttggtgtctt cttagcggtt tatgtgatga ttttaatcta
       61 ttttgaaata ctaacattga cttctcaccg ttggcttaat agattaattg ttgtcgtact
      121 aatgttttta aatggattcg gaatttatat tggtcgtttc ttacgcttac attccgttta
      181 tttctttaat gaacccttac atgttctaaa tcacattcta gaaagtctaa cgcttaaaac
      241 tgctatgttt gtatgtttta tggttgtgat gcaagcagca attctattat ttggaaaagg
      301 agtgcgtttg aacaagtgac aataaatata atcgttttaa tagtatctat cattgtcttt
      361 caattgatta ttggacacat atggcatgat attggtttaa gttatttaag aagtatctta
      421 ctcatgatgc taccatttgg actaggtgta tttatacaac aagtgtcgta ttacgaacga
      481 caatatccta aatggcaagt gcctcaaaac ataaaagtta gattgaaata tatctatctc
      541 gctacatttt tagagtatgt cgtactatac ctcactttat ttacggacat actaagataa
      601 caccaaatta ataagaaaac taaatacaac ttatatattt gagcaaagcc aatttctatc
      661 acatcaataa tagaaattgg cttatttata ttgcttaaga actttaaatt ctgctccaga
      721 ttttactcat gcctgatcta tcaattcagc cgaattagtc tagcgcttta aatgcagcta
      781 aaacttgtgt acgtttttct tgatgatcta ccatttgtct tggataatga cgacctaatt
      841 caatccccag ttcaaaaagg ttggactcat tgcgttgtgt atcatgcaaa tatttagcat
      901 caatttgatt aaaaatcgga agatatgttt tgatatacaa agctttagga tcaaagcgtt
      961 cactctgtct tattggatta aacattctaa aatacggcac tgcatccgta cctgtagaag
     1021 cagaccattg ccatccatga atatttgatg ctgcatcata gtcaataagg tgctttctaa
     1081 agaatttttc tccccatgtc caatctataa ataaatcttt ggttaaaaat tgcgacacaa
     1141 ccattctcat tcgattgtgc ataaaaccag tttgtgtcaa ttccattatt gctgcatcaa
     1201 tgattggaaa tcctgtttgc ccttcgcacc atgcattaaa atccgcttca ttttgcgacc
     1261 attttatctg tcgatattta gatttgaaag cttggtatga ggtttctgga tactgtgtca
     1321 ttaacacata ataaaattct ctaaaaatga gttcacgtat aaatgcttca tagtttgcct
     1381 catcctcatc ataacgcgcc aataaatcat taaaaatttc aataatatct aataatccat
     1441 atgctaaagc aacacttagc tgacttgtta acacttcagg taaatagtct cttccgcttt
     1501 cgtaattctg tatatcttga tctaaaaaat gttgccattt attctgttca attatttgtg
     1561 aggatccaaa tgctatgtca tcaaacacta aatcatcagg tgatgcaatg acgaagtctt
     1621 cgaatgattt taaatcatag tgatatacgt ctctatgcct caagtaaggt cgccattttt
     1681 tagagaaact agtaaaaact ttgtatggct ccccttgttg attcattgtt ttactcgctt
     1741 taaagtaatg attcccacgt atcaaagtaa cggcaatgcg tgcttcattg aataaagagc
     1801 gttgatgtaa aatatcatat tcttcaaggt gataactcat aatatcacct gcaatcacta
     1861 cttcagagat attgttttct ttacaaaatg aaatgatatc gtcataagac atgacataag
     1921 gtgtgagctt ttctgcgtgc aacgattggt aaaagcgctg caaagtaccg cgataaaact
     1981 gtgctttcaa atcagaagct gaagacatgt cctcttccgg aatgataaaa taacatgcat
     2041 caatgtcttc tttttgttga taaatatatt caaataatgg attgtgttcc attcgaaaca
     2101 ttcgatttaa taacacagct attgccataa acaaaacacc cttcaattat ctttttattt
     2161 tattaaactt tttgtcactt taaaataacc tagattctta tttatatttt aaattaaagc
     2221 aaacctaacc atttccaata agtcatacta aaaactatca ctagaatata ccctacaatt
     2281 gtcaatggta tacctgtctt caagaaatct ttcacagtaa aagtaccagt gccataagcc
     2341 aacatatttt gaggtgcact aacaggtaat aagaaaccaa aactaataac aaattgttga
     2401 attaaaacaa atcctataga ctggtctcct aagtgtaacg tagaggttag cgaaataaaa
     2461 acaggtatta acgctgatga taaacttgtt gcactcgcaa agcccaaatg aatcaatata
     2521 ttaaaaagcg tgataagtgc aattgtcgcg ataataggta aatgttttaa acctaaaaca
     2581 ccaaaagttt gatcacttaa ccattgagct gctcctgttt tcaaaagaac gttacctagt
     2641 gaaataccta caccaaacac gataattgtt ccccatggta ttttattttc aacatgtttc
     2701 catgtcatga caccaatttt cggcattaac ataacaccta aagcaataat agtaatggat
     2761 gcagagtcaa tcggatgtaa tactttttca gttgaccaaa acagtaataa taacatcgat
     2821 ataacaatta aacgccattc acgtgcgcta acggggccaa gtttatgcaa ttcttctttt
     2881 attaaatctt tgccaccttc tattgtatta atttctggag gcatcacttt aatcatgatg
     2941 aaatataaag ctatagacat aactatggac caaggcgctg catataagaa ccactcgccc
     3001 catgaaacat caaatcctaa ttgatggttt ataaaattaa tcgctacgat attttgtgct
     3061 gccgccgttt tgataccaat attccaaatt gacacagctt gtactgaagt aattattaat
     3121 aaagacgcta acttgctatc tttagaaact ttaaatgctg caatcatacc cagcaagatt
     3181 ggtacaactg cccctgctct agctgttgca gaaggaacga aaaatgcaag tacaattgaa
     3241 acaataattg ctccaataac tatatttcta gttttattac caacaattga tagcactaaa
     3301 agagctagtc ttttatgcaa attcgtttct tgcatagcag cagccaaaaa taatgcggct
     3361 gcaacgagag ctacagctga tgttgcaaat ccactaaatg ctaatgataa tgcatgattt
     3421 gttcctagaa gatcacttcc agctaaaaca gcactgccac ttttcggatt acctagcttc
     3481 tcccctaaat tttgaactgg actaaatcct aaaagtaata tcattaaacc aataattaaa
     3541 gttgctgaca ccggatatga tacagcttcc gttacccaca taataactgc aaaagctaaa
     3601 atagctagta ctgccttagc cattattggc agacttgaag gtgccgggag taatagtaca
     3661 actataagta ttataaaact cataacaatc caaaatggct tgtattgctt ttttgtcttt
     3721 tctttctttt gttgttcatg tttaactacc ttttttactt ctgacataac agtcacccct
     3781 ttgtttaatc ttaatttaat tataacaatt tttataaaca tccttttttg atttatgcaa
     3841 aatacacaaa tttatcgaaa tattttgtaa ataatattgc taggtgatga gttatctcgg
     3901 ttaattacaa aacgatatct aggtttaaat attagatcat aaaaaaacta tctactgaat
     3961 atcctcatag catcattttt ctaagcaaaa gttaaactta gttagataaa atgcaaatga
     4021 gattattgta gatagtctct ttttaatatt taaaaatgat taatgccata aacataaata
     4081 attccaaaga atgccgcgcc actaagcact gttaatataa gacttagtaa caatgtgcgt
     4141 ttataatgtt taataaatgc tatgaacaaa atgactacat tatacgcaaa gaaaagtccg
     4201 aataatgtca ttgttatttt caaatcagaa tagaaaatat ttaatagtaa tacaatgact
     4261 gcataaatta catacggaat aactataaat ttgcgttcaa atcttaattg ttcgaaacgt
     4321 ttatcttcgt ttgtcatgat atgctccttt atgatttaaa agtaaataag tttacggaat
     4381 atcttgtccg ataatagcgg tgtaaatgtc aaaccacgac tgatcatcta aattaagttg
     4441 cagcccttcg attgcttgat caatacgctt taactgactt gttccaagta tcggcatgat
     4501 acgatgcggt atttttacta accatgctat catcacagct gtgtcactca caccatattt
     4561 gtcagctatt gattgaataa ctttcataat acgttgcgct ttaatatctt ccttgtcgaa
     4621 aattttaccg cctgcaaaag gactccaagc catgatttga acatggtttt gatacattga
     4681 atccattgtt ccatcttgta aactatcaac gtgatatggc gataattcta attgattgat
     4741 gctaatatgt agtctttctt tcataatata ttgatttaac aattggtatt gtgaatgatt
     4801 aaaattcgac accccgaatg acttcaactt accttgttta acaagtttag ttaatgcatc
     4861 agcaacttgt tctggatcca tcaatggtga aggacgatga atgagtagac tatctaaata
     4921 atctacattc aaattgatta atgactgttc aacagatttc acgatgtgct tactactcaa
     4981 atcataacga tgtccatttg taaaatcaaa ttgcttagaa ggcaaaatga taccacattt
     5041 cgtaacaatt tgaattttat ttcttaattc gggtgataaa tccaaagcat taccaaacag
     5101 tgattcacat tgataatccc cataaatatc agcatgatcc atcgttgtaa tcccacgttc
     5161 aactaattca tttataaaat aatttaactc tttcgcagtc atcttccatt catttgcacg
     5221 ccaaaaacct tgtacaagcc tagaaaaatg aacatagtga ttaatcatta tttgttccat
     5281 atctcatcca ctccatccct caatgtcaca aaatattcac aataaaagta ttaagaaatt
     5341 aatgtgctat ttccgttaaa ttgaaacaac acattttctg atttacgtaa attgtagcat
     5401 atccaattga ttacgtatgt gatttgtatc taaagtttca ccaataataa caatcgttaa
     5461 tggtaattgc atgccaatta ttccataatc tggtaaacca taagcatatt gaaattcata
     5521 aattgcattt ggttgatctc taaatgatac ataacctttc aaacgtagaa cagattccga
     5581 taatttcatt ataaattgat aaaacaattg acgatcaatc ggacctgtaa atgtgtaagt
     5641 catactttta atcccatgat gatggtgatg atgcgacgat atctctctgt ctttaccagg
     5701 taagtccaat tcttccaacg aaacttgtcc ataggttgtt ttataagttg gaatacttgc
     5761 acaaatcaaa cttaattgct tatcaatttt ctcaatactg tcatcagtta caagatcaat
     5821 tttattaata ataatcatgt cacttagttt caactgatct tccatcagcg aaactgtatt
     5881 ttcggtatat tgatgacgtt ccaaaaatcg agtcgcatct aatacaccat aaataattgg
     5941 cttttcaaag aaattaacga tttgcggatc ttgacatgca actaataatt ctaaaggatg
     6001 cgcaatgcct gtcgcttcta tgatgacatg attaacgtcc ccttttaaag caatggcttt
     6061 tagttcatag acaagttctt gtttaagatc gcaacaaaca caaccattaa tcaatgaatg
     6121 gacttcaatt tcatttgaaa tactattgct atcgatatca acagtaccga attcattcat
     6181 gatgatttta attttctcat cattttttaa taattcacta atataatgtg tcagtaacgt
     6241 ggttttacca ctacccaaaa atccgttaat tatggatatt cttatttttt catctttatt
     6301 atttttcatt ttatattctc ttttctatta cagttatttt taaaaatgat aacatatata
     6361 ttaaattatc tttttgcttg atttaatcaa aaatatcttc taaactttaa acatacttta
     6421 attttagcat ggcattgaat caaagaaagt tgtgaattaa taaacaatca actttttaat
     6481 gattaccaat ccgaaaagag aaaaaaacgg atagtatgtc tggaggagaa ctttaatgtc
     6541 tgatcaacat aatttaaaag aacagctatg ctttagtttg tacaatgctc aaagacaagt
     6601 taatcgctac tactctaaca aagtttttaa gaagtacaat ctaacatacc cacaatttct
     6661 tgtcttaact attttatggg atgaatctcc tgtaaacgtc aagaaagtcg taactgaatt
     6721 agcactcgat actggtacag tatcaccatt attaaaacga atggaacaag tagacttaat
     6781 taagcgtgaa cgttccgaag tcgatcaacg tgaagtattt attcacttga ctgacaaaag
     6841 tgaaactatt agaccagaat taagtaatgc atctgacaaa gtcgcttcag cttcttcttt
     6901 atctcaagat gaagttaaag aacttaatcg cttattaggt aaagtcattc atgcatttga
     6961 tgaaacaaag gaaaaataat taacttttgt catgacaatt aaagtaatgt ttagaattta
     7021 ttaagaatag aaaaacaatt agcacgcgta agcttgttag ttaaaaaact gcttgaaagg
     7081 tttcttagcc tatcaagcag tttttttatg cattatattg aatcatattc cataaagcac
     7141 cttgattagc gagtaattgt tgatagtttc ccttttcaac tattttacca tttatcatca
     7201 caatgatggt ctcaaaacgt gacaataaag ttaaatcgtg tgtagcaaca attaatgttt
     7261 ctgcatgtgc ttcaattaaa tccataactt tcaaactatt ttgtttatct aatgccgttg
     7321 ttggttcatc taaaatccac gttgatgcag tatcttttaa taacatcctc gcaatcgcta
     7381 aacgctgaat ttctccgcca gataatgtat gaccatctaa gtcaatttga cgttctagtg
     7441 ccaaatgttc taaatctaat tgcttaaata ttgcttgcac cgcttcatct ttttcatcgg
     7501 taaataaatt ttgacgtatt gtaccatcaa ataattgttg agattgtagc aagacattta
     7561 acgcttcaaa cttatctttg tcatctattt caaacatatc catattttcg aaacgaatag
     7621 agccactatc taattgatat aaccctgcca taatttgtag taatgtactt tttcctgaac
     7681 cagaaggacc cacaatcgca atcttttcgc ctctattaac attaaaatta atatccgaca
     7741 acacatacgt ttgctggttc caatacttaa aactagcgtc cttaacttca taaatgttcg
     7801 ttgcatcata cttatcatta aggcgtttat ttccattggt agaaggtaca gatatcactt
     7861 cattaatatc gtgcaatgct tggtcagtat cggctttata atacgcgaca tttgtcattg
     7921 gtacagcttg ttcaaataaa gttaagacca ttaaaactat actcgtcata taaataatat
     7981 ttagttggcc tgcattaatt tgaattaatc ctagaactaa actaccaaaa atcgaaagca
     8041 tagcaataat atttaatata aaatcataaa tcgttaaaaa gcgttgctct ttaagttgta
     8101 atttatcaaa atgatttaat ttagccatca aattatttcg ataatgatta atttgattaa
     8161 atcgacgtag ttcatccata ccagctttat aatcataaaa atgatttaaa aatcgagcct
     8221 gttcattagc tgcatgtttc tttaaagtac gtgctttttt tgagcttaac caaggaacaa
     8281 tgagtaaagt aaacaacatg ctaaccataa tcaatagcgc atggccgatt gaaatgaacg
     8341 ccaatactat tactgtaact agcgctgtca aaccgatgac gactggtgga taataaacac
     8401 gtaaataaat attttgtaac gcctcaacac gactaatcat acgtgaaatt aaatcactag
     8461 aactcagttt acggtaaaca ttaggaatga catttactaa tttaccgaaa aactgtaccc
     8521 gaatatcacg tagcattgta aatgtagctt tatgagaaat aaggcgctct acgtatcgag
     8581 taatagctct taaaaaccca aacaatttta ctgtaacgac taaaatcatc agagcgtata
     8641 gtggcgcacc aagtgcactt tgtgtcacca tataaccact taagaaaaac atggcgagcg
     8701 caactaaact tccacaaaca ccaacaacta tagctaacaa taaatcctta tctacttgaa
     8761 attttagtcg tgttttcata agtcatcacc attttctgtt atatttactg aaatattgcg
     8821 atcatcagca atcagtcttc cattttctat atacaaacgt cgttgtaaat ggcgaattgt
     8881 attatctcta tgtgcaatga caatcatcgt tgtaccttta aaatgttgaa acagaacgtt
     8941 ctgaatcatg tgttctgttt gaatatctaa accagttgca ggctcatcaa atataacgag
     9001 at
//
LOCUS       DAMZPP010000115         8765 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMZPP010000115 DAMZPP010000000
VERSION     DAMZPP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8765
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.115"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            1..428
                     /locus_tag="POH73_002117"
                     /pseudo
     CDS             1..428
                     /locus_tag="POH73_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="exotoxin"
     gene            complement(630..899)
                     /locus_tag="POH73_002118"
     CDS             complement(630..899)
                     /locus_tag="POH73_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888047.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
     gene            1213..1899
                     /gene="mtnN"
                     /locus_tag="POH73_002119"
     CDS             1213..1899
                     /gene="mtnN"
                     /locus_tag="POH73_002119"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDI6888048.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDIIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQVPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            1919..2446
                     /locus_tag="POH73_002120"
     CDS             1919..2446
                     /locus_tag="POH73_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDI6888049.1"
                     /translation="MGLVRKFFMPNSYVKSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            2447..3547
                     /gene="yqeH"
                     /locus_tag="POH73_002121"
     CDS             2447..3547
                     /gene="yqeH"
                     /locus_tag="POH73_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDI6888050.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFGDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYSLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLI
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNLQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            3561..4367
                     /gene="aroE"
                     /locus_tag="POH73_002122"
     CDS             3561..4367
                     /gene="aroE"
                     /locus_tag="POH73_002122"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDI6888051.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELDGFNITIPHKERIIPYLDHVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            4371..4661
                     /gene="yhbY"
                     /locus_tag="POH73_002123"
     CDS             4371..4661
                     /gene="yhbY"
                     /locus_tag="POH73_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDI6888052.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATHSELVQVIGSMIVIYRESKENKEIELP"
     gene            4664..5233
                     /gene="nadD"
                     /locus_tag="POH73_002124"
     CDS             4664..5233
                     /gene="nadD"
                     /locus_tag="POH73_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HDI6888053.1"
                     /translation="MKRIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMVIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENGMIAIQIPRVDISSTMIR
                     QRVSKGKSIQVLVPKSVENYIKGEGLYEH"
     gene            5223..5807
                     /gene="yqeK"
                     /locus_tag="POH73_002125"
     CDS             5223..5807
                     /gene="yqeK"
                     /locus_tag="POH73_002125"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDI6888054.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            5808..6161
                     /gene="rsfS"
                     /locus_tag="POH73_002126"
     CDS             5808..6161
                     /gene="rsfS"
                     /locus_tag="POH73_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDI6888055.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYGQVAY"
     gene            6164..6880
                     /locus_tag="POH73_002127"
     CDS             6164..6880
                     /locus_tag="POH73_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6888056.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYRHLADSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLTQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            6920..7606
                     /locus_tag="POH73_002128"
     CDS             6920..7606
                     /locus_tag="POH73_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDI6888057.1"
                     /translation="MVLLYQFLLRYKDFLAQWKLYIISAVVLIMVLIGFIFWRQDDYT
                     SRDFENKDTVLKQSTSENSSLSKLEDVQVKDGDNSKNKGPVYVDIKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQFGANSVHVKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLNSYFTT"
     gene            7698..8159
                     /locus_tag="POH73_002129"
     CDS             7698..8159
                     /locus_tag="POH73_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDI6888058.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAEYLTKG"
     gene            8164..>8765
                     /locus_tag="POH73_002130"
     CDS             8164..>8765
                     /locus_tag="POH73_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDI6888059.1"
                     /translation="MLYVALSMIVGVLWNSSKVLSTFLFILLLYIAYRKNKIVYAPIS
                     LFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTNKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVK"
BASE COUNT         3238 a         1078 c         1649 g         2800 t
ORIGIN      
        1 ctggaagcta tagctatgaa tgtcatgggg gagaaaccaa taaaacgcaa tgtagttatg
       61 gtggtgttac tttaagtgac aataataaat acgatgatta taagaatata ccttgtaatt
      121 tatggattga cggacatcaa acagaaatag aactaactgc agtgaaaacg aaaaagtaaa
      181 ttgttatcat tcaagaatta gatgttcaat taagaaatta tttgaatgag aagtataagt
      241 tgtacgaaca aggtggcgac attgttaaag ggtatgttaa atatcataat gatgatgaaa
      301 aaaatgtaga atatgatttt tataatttaa atggtgagta tggtcatgag gtattaaaaa
      361 tgtatgctga taataaaact atcaatagtg acaaattgca tttagatatc tatttattca
      421 aatcataatt taagtgagtt aatcattaat gatctttgac agtgaaatat aaagtacctg
      481 tcattatatt gttacaaata aaaacagcag taaggaggct attactcaga aaagtcttac
      541 tgctgttttc tattagaata atctttaatg cacacaggaa ttcgatacga aacaaatatg
      601 attaactgta taattacaaa ataaaactac tatagtaaaa agaagataaa gacgatgata
      661 ataaatgcat cgaatataat tttcttaatg tttttatcaa caacatcagg taaggacata
      721 agtataaatc ctagaattag agtgtagcga catatgttca taattgttgc tgtaaactcg
      781 atattaaatg aaaactttaa aaatatactt ataaataaaa tgacaaacga cgcaattgca
      841 ataaaactgc cgtatgtagc tatctttgtt acgagactat tttgattatt ttcagccatt
      901 gtaaaatcct cttatttcat cattattatt ttaaacatat cattttcctg ttatttaata
      961 aagtttcttg acactattgg ttgatgtaat tgatatgatt agtttttgaa aaatacacaa
     1021 attagaagca tctatttcac tgttatgagt tcataaataa ttatttatga taccaaggta
     1081 ttgtgaaagc tttttataaa aataaagcaa aagttttata cagtaatgaa aatcatgata
     1141 cttgtcgttg tagtgattaa aatgtgataa aataacaaat gctaggattt tttaaaagag
     1201 gtgaatcaaa ggatgattgg tataattggt gccatggaag aagaagtaac aatattaaaa
     1261 aataaattaa cacaattaag cgaaatttca gttgcacatg ttaaatttta tactggcatt
     1321 ttaaaagata gagaagtagt gattacccaa agcggcattg gaaaagttaa tgctgcaatt
     1381 tctacgacat tattaattaa taagtttaaa ccggacatca ttattaatac aggttctgct
     1441 ggagctttag atgaaagttt aaatgtaggt gacgttctta taagtgatga tgtaaaatat
     1501 catgatgcgg atgcaacagc atttggttat gaatatggac aagtaccaca gatgccggta
     1561 gcatttcaat caagtaaacc tttaatagaa aaagtatctc aagttgtaca acaacaacaa
     1621 ttaacagcta aagtaggctt aattgtaagt ggtgatagct ttatcggtag tgttgaacaa
     1681 cgccaaaaaa ttaaaaaagc atttccaaat gcgatggcgg ttgaaatgga agcaactgca
     1741 attgcacaaa catgttatca atttaatgta ccatttgttg tagttcgtgc agtttcagac
     1801 ttagcaaatg gagaagcgga aatgagtttc gaagcatttt tagaaaaagc agcggtatca
     1861 tcaagtcaaa ctgttgaagc attagtgtct caattataaa aaggtaaagg tgagttaaat
     1921 gggtttagtt cgcaagtttt ttatgccgaa ttcatatgtt aaatcaatat ttcaaattga
     1981 tttagacaag ttagtggaca aaggcgttaa aggtattatt acagatttag ataatacgct
     2041 agtaggttgg gatgttaaag aacctacaga acgtgttaaa gcatggttta aggaagctaa
     2101 tgaaaaagga atcactatta caattgtgtc taataataat gagtctcgtg ttgctagttt
     2161 tagtcagcat ttagacatcg attttatttt taaagcgaga aagccaatgg gaaaagcgtt
     2221 tgataaagca ataactaaga tgaatataag acctgatcaa actgttgtta taggtgacca
     2281 aatgcttact gatgtatttg gtggtaaccg tcgaggttta tatacaatta tggtcgttcc
     2341 agttaaacga acggatggct ttattactaa gtttaataga ttaattgaaa gacgattatt
     2401 acgtcatttc agtaaaaaag gttatatcac atgggaggaa aattgattgt ctgacatttt
     2461 aaaatgtatc ggttgtggtg cgccacttca atctgaagat aaaaataaac ctgggtttgt
     2521 accagagcat aatatgtttg gtgatgacgt gatttgcaga cgttgtttcc gcttgaaaaa
     2581 ttataacgaa gttcaagatg taggattaga aagtgaagac tttttaaaat tattatcagg
     2641 acttgcggat aaaaagggta ttgtcgtcaa tgttgtggat gtatttgact ttgaaggatc
     2701 atttattaat gcagttaaac gtattgtcgg aaacaaaaaa atcattttag cagcaaataa
     2761 attggattta ttgccgaagc aaattaataa acgtcgagtt aaagaatggc taaaacgaac
     2821 agcaagaaaa tatagtttgg aagctgacga tgtcgtatta atttcagctg aaaaaggctg
     2881 gggcatagac gacttattat catcaattgc gaatattcga gaaaatgaag atgtgtatat
     2941 tgtagggaca acgaatgttg ggaaatctac attgattaat aaactgattg aagctagtgt
     3001 tggtgaaaaa gatgtagtaa caacttcaag attccctgga acaactttag atatgataga
     3061 tattccatta gatgaaacat catttatgta tgatacacca ggtattattc aagatcatca
     3121 aatgacgcat ttagttagtg aaaaagaatt gaaaattatt atgcctaaga aagaaataaa
     3181 acaacgcgta tatcaattaa acgaggcgca aacattattc ttcggcggtc tagcgcgcat
     3241 agattatgta tcaggtggta aacgtccgtt aatttgtttc ttttctaatg acttgaatat
     3301 acatcgtact aaaacggaga aggccaatga tttatggcgt aatcaacttg gcgatttatt
     3361 aacgccacct ggaaatctac aaaattttga tcttaatgag gtaaaggctg ttagacttga
     3421 aacaggcaaa gagaaacgcg atgttatgat ctctggtcta ggctttataa ctataggacc
     3481 aggagctaaa gtaatcgttc gtgttcctaa aaatgttgat gttgtattaa gaaattctat
     3541 tttataaggt gatttaaaaa atgaaatttg cagttatcgg aaatcctatt tcacattcct
     3601 tgtcgcccgt tatgcataga gcaaatttta attctttagg attagatgat acttatgaag
     3661 ctttaaatat tccaattgaa gattttcatt taattaaaga aattatttca aaaaaagaat
     3721 tagatggctt taatatcaca attcctcata aagagcgtat cataccgtat ttagatcatg
     3781 ttgatgaaca agcgattaat gcaggtgcag ttaacactgt tttgataaaa gatggcaagt
     3841 ggatagggta taatacagat ggtattggtt atgttaaagg attgcacagc gtttatccag
     3901 atttagaaaa tgcatacatt ttaattttgg gagcaggtgg tgcaagtaaa ggtattgctt
     3961 atgaattagc aaaatttgta aagcccaaat taactgttgc gaatagaacg atggctcgtt
     4021 ttgaatcttg gaatttaaat ataaaccaaa tttcattggc agatgctgaa aagtatttag
     4081 ctgaattcga tattgttatt aatacaacac cagcgggtat ggctggaaat aacgaaagta
     4141 ttattaattt aaagcatctt tctcccaata ctttaatgag tgatattgtt tatataccat
     4201 ataaaacacc tattttagag gaagcagagc gcaagggaaa ccatatttat aatggcttag
     4261 atatgtttgt ttaccaaggt gcggaaagct ttaaaatttg gactaataaa gatgctgata
     4321 ttaattctat gaaaacagca gttttacaac aattaaaagg agaataacat atgcttactg
     4381 gcaaacaaaa aagatactta agaagtttag cacacaatat tgatccgatt tttcaaattg
     4441 gaaaaggcgg tatcaacgaa aacatgatta aacaaataga tgatacgtta gaaaacagag
     4501 aattgattaa agtacatgta ctacaaaata actttgatga taaaaaagaa ttagctgaaa
     4561 cattaagcga agctacgcat agtgaattag tgcaagtgat tggatctatg atcgtgattt
     4621 atagagaatc taaagaaaat aaagaaattg aattgccata ataatgaaaa ggatagtact
     4681 ttacggcggt cagtttaacc ctatccatac tgcacatatg atagtagcta gcgaagtatt
     4741 tcatgaatta cagccagatg aattttattt tttacctagt tttatgtctc cattgaaaaa
     4801 gcaccatgat tttatagacg ttcagcacag attaacaatg atacagatgg ttatcgacga
     4861 gcttggtttt ggagatattt gcgacgatga aattaaacgt ggtggtcaaa gttataccta
     4921 tgacacgatc aaggcattca aggagcaaca caaagacagt gagttgtact ttgttattgg
     4981 gacggatcag tataaccaac tagagaaatg gtatcaaatt gaatacttaa aagaaatggt
     5041 tacttttgta gttgtaaatc gagacaaaaa tagtcaaaat gttgaaaatg gtatgattgc
     5101 aattcagata cctagggtag atataagttc gacaatgatt cgacaaagag ttagtaaagg
     5161 taaatctatc caagttcttg ttcctaaatc cgttgaaaac tatattaagg gggaaggatt
     5221 atatgaacat tgaaaaagca aaacggcttg caaaagagaa actgccagag aaacgttata
     5281 atcattcttt aagagttgca gaaacagcga ttaaattagc agaaatttat gatggagaca
     5341 ctagtaaagt agaattagca ggtgtattac atgatttctg taaatatgat gatttaggta
     5401 aaatgtatca aattgttcga caatacgaat taggtaatga tctactgagt tatggtagtg
     5461 aaatattgca tggcccagtg tgtgcagcaa tcatggaaca tgaatatggt atcaatgatg
     5521 aggaagtatt aatggctatc aaataccata ctacgggacg tcaacaaatg acaaaaactg
     5581 aaaaactgat ttttattgca gattacatcg aacctggtag aacaatccca ggagttgatg
     5641 atattcgaga tatggcatac aatcaaggta gtttagataa gacaatttat gaaatttcta
     5701 aacgcacagt actattttta atacagaaag atattacggt atataataag acaattgact
     5761 gtttaaatta ttataactat agtgacgaaa gaataaagga tgattaaatg aattcacaag
     5821 aattattagc aattgctgtg gatgcaattg acaataaaaa aggcgaagat acgatttctt
     5881 tagaaatgaa aggtatcagc gatatgacag attattttgt tgtaacgcac ggaaataatg
     5941 aacgacaagt tcaagcgatt gctagagcgg tgaaagaagt agccaatgaa caaaatatag
     6001 aagtaaaacg tatggaaggt tacaatgaag cgcgttggat attaattgac ttagctgatg
     6061 ttgtggtaca tgttttccat aaagacgaac gaaattatta taatattgaa aagttatatc
     6121 aagatgcacc attagaatca tatggtcagg ttgcgtatta attatgtcgc aatatgcaga
     6181 aatgagccta gtgtacgatc aattgactca agatcaacca tatgaaaaat ggtttgaaat
     6241 tgtaaaaaat cactgcaaag atgaatcaaa tattttagat attggatgcg gtactggtag
     6301 tttaacagtt caattagaag ctttaggtaa tgttactggt atggatttaa gtgttgatat
     6361 gttgactgtt gcagctcaaa aatcagcaaa tgtaaattgg cttgaaggag atatgacatc
     6421 gtttgatttg caacaacaat ttgatatcat cactattttt tgtgactctc taaattattt
     6481 gcaagatgaa actgctgtta ttgaaacatt catcaatgtt tatcgtcatc tggctgattc
     6541 aggtgtattt atttttgatg tacatactgt ttataaaatg atgactttgt ttaataatca
     6601 aagttatatt gatgataaag gggacatttt tttagcatgg gatgctgtgc aaggagattt
     6661 acctttaagt gtttatcatg atatgacatt tttcattcga catgaagatg aaacatattc
     6721 aagatttgac gagtctcatt ttcagagaac atttgatgaa aagacatatt tgtcttggtt
     6781 aactcaagtt ggttttaaac atgttgaaac atttactgat tttaatatag atgaacataa
     6841 tgaagatgca gaaagattgt ttttcattgc gaaaaaataa aagtaaaacc actcgctcag
     6901 acgtgtataa agttgaggag tggttttatt gtatcaattt ttattacgct ataaagattt
     6961 tttagctcaa tggaagttat atattataag tgctgttgtt ttaattatgg tattaattgg
     7021 ttttatattc tggagacaag atgattatac ttcaagagat tttgaaaata aagatactgt
     7081 tctgaaacaa agcactagtg aaaatagtag tttgtccaaa ttagaagatg tccaggtcaa
     7141 agatggagat aattccaaaa ataaaggtcc tgtatatgtc gatataaaag gtgctgttaa
     7201 acatcctaat gtttataaaa tgacatctaa ggatagagta gttgatttac ttgataaagc
     7261 acaattatta gatgatgcag atgtaagtca aattaatttg tctgaaaaat taacagatca
     7321 aaaaatgatt ttcattcctc ataaaggaca aaagaatgtt gaaccacaat ttggagcaaa
     7381 cagtgtgcac gtaaaaaatg ggaacacaaa taatactaaa gtaaatttaa atacggcatc
     7441 tgtatcagaa ttgatgtctg ttcctggagt agggcaagct aaagctaatg caattgttga
     7501 atatcgcaac caacaaggtg catttcaaga aattgacgat ttgaaaaaag taaaaggttt
     7561 tggaagtaaa acttttgata aactgaattc ttatttcacg acataatgga ttattttaat
     7621 tgcaaatttt attttttgtg attaatctta tattattaga aaatgttact aaattttaag
     7681 tatggaaggt gaacatcttg gaaagaatca aatgggaaga atattttatg gcacaaagtc
     7741 atttgctagc attacgttca acttgtcaaa gattatctgt aggtgcaacg attgttaagg
     7801 ataatcgtat tattgctggt ggttataatg gctctgtagc tggcgaggtg cattgtatag
     7861 atgaagggtg tttaattgaa gatggacatt gtatcagaac gatacatgca gaaatgaatg
     7921 ctttattaca atgtgcaaaa caaggtgtat ctactgaagg tgcaacaatc tatgttactc
     7981 attttccatg cctaaattgt acaaagtcaa ttattcaagc aggtataaag cgtatctact
     8041 atgcagaaga ttatcataac catgaatatg caactaaatt actcaaacaa tctggtattg
     8101 aatttaaaaa aattccattt tcacctgaat atgttgctga atatctgact aaaggttgat
     8161 gttttgctgt atgtcgcgtt atcaatgatt gtaggagtgc tttggaattc tagcaaagtg
     8221 ctctctacat ttctttttat tttacttttg tatattgctt atcgtaaaaa taaaatcgta
     8281 tatgccccta tttctctctt tttaatcatt ttctcctcat ggtatttaca ttattcacaa
     8341 caagcaatat ttaattatat caattatatt gaacgtaatt ctcagtttaa tgagcgtgct
     8401 caagtaatcc aaattcaacg tcaaggtagt gacacatata aaggtaggtt gagtttaaaa
     8461 aatgaaatat atcctttctt tttaacaaat aaaaagaatt ttgatttaaa gaaaattgaa
     8521 agtcgtaatt gtattgttaa aggacaattc aaagttaatg acaataagtt tgtaactctt
     8581 aaattacaaa gtatagttgt acaaagctgc ctagaatcga accggtctaa tttaattgag
     8641 aaacataaac agtttataat gaatcgaatt tatgattcgg gtattaagtt tccggatcgt
     8701 attatggcat tgattactgg tgacgtaaaa gaaattaatg agcaatttaa ggaacgtgtt
     8761 aaaga
//
LOCUS       DAMZPP010000116         8747 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMZPP010000116 DAMZPP010000000
VERSION     DAMZPP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8747
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.116"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..533
                     /locus_tag="POH73_002131"
     CDS             <1..533
                     /locus_tag="POH73_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888060.1"
                     /translation="KTTEYARKTRRSVDLDKIRAKRDKFKKKVNDIIDIQEDDIPDWM
                     RKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFVSGDWKLSQTQYVIEWVTLLILLF
                     IIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLTEFINIMIHGLLLSVFITPTLLLM
                     LLAIIISYSLQLRERP"
     gene            958..2130
                     /locus_tag="POH73_002132"
     CDS             958..2130
                     /locus_tag="POH73_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDI6888061.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQEVNTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSVIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRIFSVQKDYDKLITGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            2184..2726
                     /locus_tag="POH73_002133"
     CDS             2184..2726
                     /locus_tag="POH73_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HDI6888062.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRER
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISSAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            2880..3146
                     /locus_tag="POH73_002134"
     CDS             2880..3146
                     /locus_tag="POH73_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDI6888063.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            3149..4867
                     /gene="ptsP"
                     /locus_tag="POH73_002135"
     CDS             3149..4867
                     /gene="ptsP"
                     /locus_tag="POH73_002135"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDI6888064.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNNAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ASALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEAKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMDGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(5104..5337)
                     /gene="nrdH"
                     /locus_tag="POH73_002136"
     CDS             complement(5104..5337)
                     /gene="nrdH"
                     /locus_tag="POH73_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDI6888065.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNERQIDFEERNIKNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            5535..6896
                     /locus_tag="POH73_002137"
     CDS             5535..6896
                     /locus_tag="POH73_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDI6888066.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRNFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYTILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            6893..7912
                     /locus_tag="POH73_002138"
     CDS             6893..7912
                     /locus_tag="POH73_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDI6888067.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFAISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            8045..8707
                     /locus_tag="POH73_002139"
     CDS             8045..8707
                     /locus_tag="POH73_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDI6888068.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
BASE COUNT         3006 a         1155 c         1627 g         2959 t
ORIGIN      
        1 ataagactac agaatatgca cgtaagacaa gaagaagtgt cgatttagat aaaattagag
       61 ctaaaagaga taaattcaaa aagaaagtta atgatattat cgatattcaa gaggacgata
      121 ttcctgattg gatgagaaaa ccgaaatggg ttaaaccgat gtatgtcgaa ctattttgtg
      181 gtgtcgtcat ctttttattc acatttttag aatttaataa tcgtaatgca ttatttgtat
      241 ctggtgattg gaaattatca cagacacaat atgttattga atgggttaca ttactaattc
      301 tgctattcat tattatcgca tatatcgcta caacgttaac tttccacttg aaaggtaagt
      361 tttattattt acaattattt atggggagca ttttattctt taaattgtta acggaattta
      421 taaatataat gattcatgga cttctacttt cagtgttcat tacgccaaca ttactattaa
      481 tgttattggc aatcatcatt tcttattcgt tacaattacg agagcgacca taattaaaag
      541 cattataaaa gtactatcta ttaaacattt tgatgtgtac gctataagtt agatatatct
      601 ctaacttact tagatacagg tcaatgaagt ttatggatag tacttttttt gtaactagat
      661 ttgattgatt caggtaatgt gaattaagta ttgaaaattg tatacaaagt tttagtgcaa
      721 ataaaatagt tgaaaagtta tccatttgta aaattaagaa aactagtaaa tagttgaagt
      781 gacaagtgga atttgcgaaa cgatatatag tatctcattt atagaagttt aacatatatc
      841 attcgaatta ctaatttgtt aaaatcaaca gtaagattag aagtagatga tattgaaatt
      901 tggcaaacaa tttaatctat ataaaactac aacgaaacac agaaaggaag ttgtcagatg
      961 aaaatagcaa ctctgaacaa aggcaaagaa acaaaatatt ttaatggata tcctttaatt
     1021 gaagaagagg atatctattc acaagatcat ttaaaagaag gagatatttt tcaaattgtg
     1081 actgataaat cacaatatgt tgcaacggct tatgttggtc gccaacataa aggattaggt
     1141 tgggttctaa catacgataa agctcaagaa gtcaacacag ctttctttgt gaaattgttt
     1201 aatactgcat tagcagaacg tgattattat tttaatatag atggaacaaa tgcttttaga
     1261 ttatttaatg ctgaaggtga tggagttggg ggattaacga tcgacaatta cgatggtcat
     1321 ttgttgattc aatggtactc aaaaggtatt tataaattta aatatgccat tcttgaagcg
     1381 gttagaaagg tatttgatta taaatctata tacgaaaaag taagatttaa agacagcgaa
     1441 tatagtggtg gttttgttga aggcgatgca cctgagtttc caattgttat cgaagaaaac
     1501 ttcacatttt ataatgtaga ccttgaagat ggtttgatga caggtatctt tttagatcaa
     1561 aaagaagtgc gcaagaaatt aagggatcaa tatgccaaag aacgccatgt tttaaactta
     1621 tttagttata caggtgcttt ttctgtaata gcagcaagtg aggcatcttc aacaacaagt
     1681 gtagatttgg ctaatcgttc tcgtagttta actgaagaaa attttggatt aaatgctatt
     1741 gatcctaaat ctcaatatat ttatgtcatg gacacttttg atttctataa atatgctgca
     1801 cgtcatggac atagttatga cacgatcgtg attgatccac ctagctttgc acgcaataaa
     1861 aaacgtatat tttcagtgca aaaagattat gacaaattaa ttactggcgc cttaaatatc
     1921 ttatcatctg aaggaacatt attattatgt acaaacgcaa gtgtatatcc attaaagcaa
     1981 tttaaaaata ctattaaaaa gactcttgaa gagagtggcg ttgattatga attaactgaa
     2041 gttatgggat taccaaaaga ttttaaaaca catccacatt ataagccatc taaatattta
     2101 aaagctgttt ttgtaaatat tagacattaa tttgataaaa aggggtatag tatcttattg
     2161 agaaaaagaa gggtgatgat acaatgggat tcaaaaataa tttaacatca aatttaacaa
     2221 ataaaattgg taattcagtt tttaaaatag aaaatgttga cggaaaaggt gcaatgccaa
     2281 cgacgattca agaattgaga gaaagacgcg aacgtgctga agcaattgta aagagaaagt
     2341 ctttaatgtc atcaacaatg agcgttgttc caattcctgg attagacttt ggtgttgatt
     2401 taaaattaat gaaagatatt atcgaagatg taaataaaat atacggatta gatcataagc
     2461 aagttaatag ccttggggat gatgtgaaag aaagaattat gtctgcagcg gcaattcaag
     2521 gtagtcaatt tattggtaaa agaatttcaa gtgcattttt aaaaattgta attagagatg
     2581 tagcaaaacg aactgcagct aaacaaacaa aatggtttcc tgttgtagga caagctgtgt
     2641 ctgcatctat tagttactat tttatgaata aaattggaaa agatcacatt caaaaatgcg
     2701 aaaatgttat taaaaatgtc atgtaggtgc tataatagtt ttgcaatttg caaattttac
     2761 tgaaaccggt tttaaacgaa ttgaatttaa agcatggttt tggtaaagtt aaggtataaa
     2821 actaagttag tattgtaata atattgaaga ttctaactat acgaaggaga aatgtaatta
     2881 tggaacaaaa ttcatatgta atcatcgacg agactggtat tcacgctaga ccagcaacaa
     2941 tgttagttca aacagcttca aaattcgatt ctgatatcca attagaatat aacggtaaaa
     3001 aggttaactt aaaatcaatc atgggtgtta tgagccttgg tgttggtaaa gatgctgaaa
     3061 ttacaattta tgctgacggt agtgatgaat ctgacgccat tcaagcaatc agtgacgtct
     3121 tatcaaaaga aggattgact aaataatcat gtctaaatta attaaaggaa ttgctgcatc
     3181 tgatggtgtt gcaattgcta aagcttattt gttagttgag ccagacttaa cattcgacaa
     3241 aaatgaaaaa gtcactgatg ttgaaggaga agttgctaag ttcaataacg ctatcgaagc
     3301 ttctaaagtt gagttaacta aaattagaaa taatgcagag gttcaattag gtgctgataa
     3361 agctgctatc tttgatgcac atttattagt tttagatgac cctgaattaa ttcaaccaat
     3421 tcaagataag attaaaaatg aaaacgctaa tgctgcttca gcattaacag atgtaacaac
     3481 acaatttgtt acgatttttg aatctatgga taatgaatac atgaaagaac gtgcggctga
     3541 tattcgcgac gtttctaaac gtgtgttatc acatatttta ggtgtagaat taccgaatcc
     3601 gagtatgatt gatgaaagcg ttgttattgt agggaatgac ttaacgccat cagatactgc
     3661 tcaattaaat aaagaattcg tacaaggttt tgctacaaac attggcggaa gaacaagtca
     3721 ctctgcgatt atgagtcgtt ctttagaaat tccagcaatt gttggtacaa aatcaattac
     3781 tcaagaagct aaacaaggcg acatgattat cgtagatgga ttaaatggtg atgtaatcgt
     3841 taatccaact gaagatgagt taatcgctta tcaagataaa cgtgagcgtt attttgctga
     3901 caagaaagaa ttacaaaaac tacgtgatgc tgatactgtt acagttgatg gtgttcacgc
     3961 agagcttgct gcaaatattg gtacacctaa tgatttgcca ggtgttattg aaaatggtgc
     4021 acaaggtatc ggcttatata gaactgagtt tttatatatg ggtcgtgacc aaatgcctac
     4081 agaagaagaa caatttgaag cttataaaga agtattagaa gcaatggacg gtaaacgtgt
     4141 tgttgtacgt actttagata taggtggaga taaagaatta tcatacttaa acttgcctga
     4201 agaaatgaat ccattcttag gttaccgtgc gattcgttta tgccttgcgc aacaagatat
     4261 tttcagacca cagctacgtg cattattacg tgcatcagtt tatggtaagt taaatatcat
     4321 gttcccaatg gttgcaacaa ttaacgaatt tagagaagct aaagctatat tattagaaga
     4381 aaaagaaaac cttaaaaatg aaggtcatga catttcagat gatatagaat taggaatcat
     4441 ggtagagata cctgcaacag cagcattagc tgatgtcttt gctaaagaag tagatttctt
     4501 cagtatcggt acaaatgatt taattcaata cacattagct gctgaccgta tgtcagagcg
     4561 tgtatcatat ctataccaac catataatcc ttcaatctta cgtttagtta aacaagttat
     4621 tgaagcgtca cataaagaag gtaaatggac aggtatgtgt ggtgaaatgg ctggagatga
     4681 aacagctatt ccattattgc ttggtttagg tttagatgag ttctctatga gtgcaacgtc
     4741 tattctgaaa gcaagaagac aaattaatgg tttaagtaaa aatgaaatga ctgaacttgc
     4801 taaccgtgca gtcgactgcg caacgcaaga agaagttatt gaattagtta acaactacgt
     4861 aaaataatta attctttcga aagacagaat tataaataaa cccgaagtat tcattgtgag
     4921 taattatgaa tacttcgggt tctttttttg atttatgtta atttcaatgg aatgttgttg
     4981 ctaactaatt aaacaactag gtgtcattga cataaatatt gttctttaat taatgttagt
     5041 taatttaaaa atcgatattt aatttatgat agctaaaaat gatgaagtta atttagatga
     5101 aaattaatct tggatattta atactttgtt gatttcatca agatcaacat ggtacattgg
     5161 attgccattc aacaaaataa acggagttga aaaagcatca aaatctatca tttcgtttcg
     5221 atattgttga tttttgatat ttctctcttc aaaatcaatt tgacgctcat ttagataatt
     5281 ttttacaaat gtacaaggtg gacaatcatt ctgtgtataa acgattattt ctgacatgta
     5341 tcatgctcct tcgtgcttta ttctaatgta atatacacaa ttatacattg aaattcaaat
     5401 ttgtgaacac attgtgaact gacataaatt atacacaatt tcatagcgca taatgtttta
     5461 aaaatacaca ttatagcaat tataattaag ttcattgtgt tgtcatcaaa aaaagaaaaa
     5521 ggtgatgttt ttaaatggat acagttgaaa tcagtcggtt tttgacagct atgactttag
     5581 cagttcatat catttttgca acgattggtg ttggtatgcc tttaatgttc gcaattgcag
     5641 aatttttagg tattcgcaaa aatgatcttc aatatatagc tatggccaaa agatgggcta
     5701 aagcttatac aattactgta gcagtgggag ttgttacagg tacaattata ggacttcaat
     5761 tatcattgat ttggcctaca tttatggaaa tgggtggaca cgttattgca cttccattat
     5821 ttatggaaac atttgcgttc ttctttgaag ctattttctt aagtatatat ttatatactt
     5881 gggaccgttt taaaaataaa tggacacatt tcttaattag tataccagta attattggtg
     5941 gctctttctc agcattcttc attacttcag tgaattcatt tatgaatacg cctgcaggtt
     6001 ttgagttgaa gaatggaaag atggtcaatg ttcaacctat agaagcgatg tttaacccat
     6061 cgtttatagt tcgatcattt cacgtaatta ctacagccgg tatgacgatg gcatttgtta
     6121 ttgcatcaat agcagctttt aaattattgc gtaatcgtca acctaaggat actgtctacc
     6181 ataagaaagc tttgaaaatg tctatgattg ttggattctt ttcaacttta ctttctatgt
     6241 tggcagggga tttatctgca aaatttttgc ataaattcca acctgaaaaa ttagcagctt
     6301 atgaatggca tttcgataca tcttcccatg ccaaattatt attatttggt gtgttagatg
     6361 aaaagactca gcaagttaaa ggtgcgattg aattacctgg actattgagt ttcttagcag
     6421 ataatagtgt caaaactaaa gtgcaagggt taaatgattt tccgaaaagt ttacatccgc
     6481 ctatgatcgt ccattatttc tttgatttaa tggtaacgat gggaatttta tgttttgtca
     6541 tttcaggtgt ttatgtttta actttaatgt ttaaaaagct tagaaatttt tctactcata
     6601 aatggatgct ttacggaata ttattaacag gaccagcttc aatgctagct atagaatttg
     6661 gatggttctt aacagagatg ggtagacagc cttggattgt tcgtggttat atgcgcgtgg
     6721 cagaagcagc aacacaagca ggcggaataa cctttgttac aattttattt ggcatattgt
     6781 acaccatttt aatgtataca tgtgcatacg tattaattcg tatgtttaaa aataaaccgg
     6841 cgtatgaaga tgtaaatcgt ttagccaaga agcaaggagg agaaatagaa aaatgattta
     6901 tgcatttata ggtataacag tgttatggtt gtttttattt tgctatatca ttattgcttc
     6961 tatagatttt ggggcaggtt tcttcgcatt gcattcaaag ttaactggtg atgaaaagaa
     7021 aattaatcac ttaatttcac gttatttgaa cccagtttgg gaagttacga atgtattctt
     7081 tgttttcttc ttcgtaggat tcgtaggttt ctttccagag tcaatcaaat atttaggcac
     7141 ggtattgtta ataccaggtt caatagcact gattatgata tcgttgagaa acagttttta
     7201 tgcatttgaa aattatggtc aagatacaaa attagcatgg atgatcatgt atggggtaag
     7261 tggattgtta attccagctt cattatctac tgctttaact attacagaag gtggttatat
     7321 taatgttcga aacaatgtta tcgatctaga ttgggtgcag ttactgttaa gcccatttgc
     7381 ttggtctgta gtattcttgg caattatttc agttttatat atttcatcag gatttttgac
     7441 atattatgct aaaaaagcaa atgacgaacc agcatataat ttaacaagac aatggcacat
     7501 atttttaggg ccaccgatga ttatcatttg tttattcgta tttctatcat tacgcattca
     7561 aaattctgaa catttttatt cagctgtttt tgactactgg tggatgtttg ctataagttt
     7621 cctattcttt gcattagctt cattgttaac attctttaag aaaaaacatg gtttggcttt
     7681 tgtatttgtt attttacaaa tgatgttcgc gttctttggc tatggaatta gtaaattgcc
     7741 atacctttta tatccgtttg taaaaattac agatgcatat gttaatccag aaatgggctg
     7801 gacattagtg attgtcttta ttttaggttt acttttatta cttccatcgt taatattatt
     7861 attaagatta tttgttttcg acaaagaata tgttgaagga aagaaatcat aagttgagac
     7921 gataccccaa ctttcattta cttgcattac tgcatttaga taatttagtt ggggtactca
     7981 tttataataa ttttaaattt atattttcaa tgaattgatg aaaatcattt aatggagggc
     8041 gagtatgggt aaagaatatg tagtcatcgg tctaggtcgt tttggaggta gtatcgttcg
     8101 tgagttgaat gcattagaca tggatgtaat ggccatcgac catgatgaaa atagagtgaa
     8161 tgaatatagt gatatcgcta ctcatgcggt tgttgcagat actacagatg aagcagttat
     8221 gaaaagttta ggtatccgta actttgatca tgtcattgtg gcaattggtg aaaatattca
     8281 atcaagtacg ttgacgactt tgattttaaa agagttaggt gtaaaaaaag taactgctaa
     8341 agcacaaaat gattatcatg caaagatttt aaataaaatt ggagcagata cggttgtgca
     8401 ccctgagcgt gatatgggta gacgtattgc gcataatgtt gcgagtgcaa gtgtacttga
     8461 ttatcttgag ttggcagacg agcattctat tgtagaattg aaagcgactg aaaagatggc
     8521 ggggcagtct atcattgatt tagatataag agcacaatat ggaattaaca ttattgcaat
     8581 taaacgaggc aaagagttca tcatttcacc aaatccaaat attaatttag aaataggtga
     8641 tattttaatt atgattggac atgataatga tttaaatcgc tttgaaaaaa atattgcgac
     8701 gagataatcg ggagtggtac agatatgata ttttctcaaa atttatt
//
LOCUS       DAMZPP010000117         8602 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMZPP010000117 DAMZPP010000000
VERSION     DAMZPP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.117"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..456)
                     /locus_tag="POH73_002140"
     CDS             complement(<1..456)
                     /locus_tag="POH73_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HDI6888069.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSTPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFATLAAKSIVNHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVI"
     gene            734..1993
                     /gene="sdpC"
                     /locus_tag="POH73_002141"
     CDS             734..1993
                     /gene="sdpC"
                     /locus_tag="POH73_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HDI6888070.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSVIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKIQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYDTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGIAKNDTENTVADEPSIHEGTEKTES
                     QHHIDNQTESNHDEDHDITSKSVESAESVKHAPQSDDLTNDSNEDETEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            complement(2059..2745)
                     /locus_tag="POH73_002142"
     CDS             complement(2059..2745)
                     /locus_tag="POH73_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HDI6888071.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKMVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            2915..3568
                     /locus_tag="POH73_002143"
     CDS             2915..3568
                     /locus_tag="POH73_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDI6888072.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPSLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            complement(3804..4823)
                     /locus_tag="POH73_002144"
     CDS             complement(3804..4823)
                     /locus_tag="POH73_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDI6888073.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLQGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(4853..5179)
                     /locus_tag="POH73_002145"
     CDS             complement(4853..5179)
                     /locus_tag="POH73_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDI6888074.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGVILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            complement(5228..5413)
                     /locus_tag="POH73_002146"
     CDS             complement(5228..5413)
                     /locus_tag="POH73_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888075.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            complement(5397..6629)
                     /locus_tag="POH73_002147"
     CDS             complement(5397..6629)
                     /locus_tag="POH73_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6888076.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGIIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGIMAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGRLALITSYIPFLAPFQLVVRAQTSGLQLHEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            complement(6622..7521)
                     /locus_tag="POH73_002148"
     CDS             complement(6622..7521)
                     /locus_tag="POH73_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6888077.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDMSLELESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTHIRGIIHSENMKQGLQLTIENEDVSKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            complement(7754..8401)
                     /locus_tag="POH73_002149"
     CDS             complement(7754..8401)
                     /locus_tag="POH73_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDI6888078.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMMLWHLILMV
                     PAVCVLIISLFIFFISIAADNEALIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSSIKNQSVTSETPKKAGTSETPKTPEETDNQL"
BASE COUNT         2857 a         1615 c         1150 g         2980 t
ORIGIN      
        1 tatcacacca atagattgtg ttggatagac tttacgcgtt gctaaatatt gagcatacgc
       61 aatatcatgt ccaccaccta ataaaaacgt ttgtctatga ttaacaattg acttcgctgc
      121 aagtgttgca aattcttttt gagtatcaat taattcctca tgatcatgat aaacatttcc
      181 gtaatcgact aaagtttcac attgattcaa atccggcaaa cctgcaaatg cttgtttaat
      241 cgcatctggt ccttcttttg caccaatgcg ccccttgttt aaagcaacac ctttgtcaac
      301 agcatagcct aatataccaa cccctgatgg tgtactactt ttttccaatt tcgacaaatc
      361 ttcaaatgtc actgtttgaa aatgcctgaa ttttttcggg tctgtttcac tatctaacct
      421 tccagtccat aaatttggtt caccttgctt gtacacagca tttccccctc ttatttatgt
      481 ggctttttaa caattaaagt ataacgtact acaaattttg aattcaattc ataattaaaa
      541 agtacttatc tatatacctt ttacccctat tttctattca ctaacgaaca cgaaagcgat
      601 ttatgttaat aaaatacgtc aagactatta cattttcatt aatattagca cagacaattt
      661 aactcacggc ttgtaatatg tataattgtt acaaaatgat attttgcttg ttacctaatg
      721 gaggttacat ataatgaaaa acaataaaat ttctggtttt caatgggcaa tgacgatctt
      781 cgtctttttc gttattacaa tggcgttatc cattatgctt agagatttcc agtctgtcat
      841 tggtgtcaaa cactttatat ttgaagttac agatctagca ccattaattg ctgcaatcat
      901 ttgtatactc gttttcaaat ataaaaagat ccaacttgca ggtttaaaat tctcaatcag
      961 cctgaaagta attgaacgtc tactgctagc tttaatttta cctttaatta ttctaattat
     1021 tggtatgtac agctttaata catttgcaga tagctttatt ttattacaat caacaggctt
     1081 atcagtacct attacacata ttctgattgg acatattcta atggcgttcg tagtagaatt
     1141 cggattccgt tcatacttac aaaatattgt tgaaacgaaa atgaatacgt tctttgcaag
     1201 catagttgtt ggtttaatgt actctgtatt ttcagcaaat acaacttacg atacagaatt
     1261 tgcagcttat aatttccttt acacattctc attctcaatg attctaggtg agttaattag
     1321 agctacaaaa ggacgtacga tctacatcgc aacaacattt catgcttcaa tgacatttgg
     1381 ccttattttc ttatttagcg aagaaatcgg tgatttattt tcaattaagg tcattgctat
     1441 ttcaacagca attgtagcgg ttggctatat tggtttaagc ttaattattc gaggtattgc
     1501 ttatttaaca actagacgaa accttgaaga acttgagcct aataattatt tagaccatgt
     1561 caatgacgat gaagaaacta atcatactga ggctgaaaaa tcttcttcaa atattaaaga
     1621 tgctgaaaaa acaggtgtag ctactgcatc aacggtcggt attgctaaaa atgatactga
     1681 aaatactgtg gctgacgaac caagcattca tgaaggtact gaaaaaacag aatctcaaca
     1741 tcacatagat aatcaaactg aatctaatca tgatgaagat catgacatca cttcgaagtc
     1801 agtagaatca gcagaatcag ttaaacatgc accacaaagt gacgatttaa caaacgattc
     1861 aaatgaagat gaaacagagc aatcattaaa agaacctgcg acctataaag aagacagacg
     1921 ttcatcagtt gtaatcgatg cagaaaaaca tatcgaaaaa actgaagaac aatcttcaga
     1981 taaaaacaaa taataacaaa gcgcttgcta gtacctctta aaaagatgat gctagcaagc
     2041 gcttttctat actatatatt atttttctaa aattttaacg ccctcttgag tacctacaat
     2101 aacttgatct gccatatcta agaagtagcc tgtctcaaac acacctgtca gatgaattaa
     2161 atactcatgg aacttatatg ggtcaattcc ttttggtaac ttacaatcta aaatataatt
     2221 gccattatca gttataaatg cgacatcttc gtttacacgg cgttctactt ttatatcagc
     2281 gtatgattca attttacgta atatgtgata ccagttaaat ttatccacct ctactggtaa
     2341 cttaaacgtc tcacctaagt attgaaccat tttcgtttca tcgacaacca caacaaaacg
     2401 tgatgccatt tcatctatca ctttctctct gaacagcgca ccaccaccac ctttaattat
     2461 atttaaagat ggatctactt catcagcacc atcaattgct aagtcgatat gatcaacatc
     2521 attgatttcg catattttaa tacctagttc ttttgctaaa aatgcaattt tattagaagt
     2581 gcatacacct gtaatattgt aaccacgttc tttaattagt tgcgccattt gaggtaagag
     2641 taattccatt gtacttcctg taccaattcc cagcgtcatg tcaccattga tttgacttaa
     2701 aacatcattt aatgtcatta acttgagtgc tttgacatct ttcatgaagg tagcctccca
     2761 tatttatgta atctattcaa ttcatatttt acatgactcg tataaattaa cataccctta
     2821 tttctaacat tttttaaaca tatcaatcag aatttacaat tctaaaaatt ttttatagta
     2881 caatatactt taacttaacg ttaaggagaa tgatatgata tcaattcacg caatttcgac
     2941 aggaaaaatc caagatttgc cttatagctc gaaaagaccg atgcgctctg ctttagataa
     3001 aactaagatt tcacaaacaa catggttatc ttcaactggt ttcactggtg atgaacaggc
     3061 ttataaagat catggtgggc cacataaagc agtttgtggg tttagtaaac ataactatgc
     3121 actgtatcaa gatgatttac cttcactacc tactcatgcg atgtttggag agaatttaac
     3181 atttgattat ttagacgaat ctgatgttta ctttggtaat caatatcgtt taggtgaagc
     3241 gttaattgag gtttctgaaa ttagagaacc atgctggaaa attcaagcaa aatataatat
     3301 tcctgattta gtgaagcgca tgtctacatc tggtaaaaca ggtttctatt tccgggtatt
     3361 aaaacaaggc tatgtatctc caaatgatca gctttactta atacaagaag caccaatcga
     3421 acatcgttta tctgtacaac agcttaatga cctttattat aatgatagac aaaatcaaga
     3481 tatgttacga tatgcactaa acaatccatt tctgtcacca acaagacgcg ataaacttca
     3541 aaaaatgtat aacagaacat tgaaataatt acctttcatt ataaatgtta aatgaacttt
     3601 tcaaaacaaa aaggaatcaa cttacacatc gtttgtataa tagtcttatc tataactact
     3661 gatgctattg attccttttt tatgttatat attttcttta tttcaaactg tatattaatg
     3721 atgtcacttc atttgatacg attcttgata acctattcaa aattccgcca aataacataa
     3781 atattatata aatgccgata cttttaatca ttttctactt tttcttcgat acggaaactt
     3841 gttttcgaat tgaacacttc accagctttt aaaattgacg gtgctttttc accatataaa
     3901 ttaatatcat ttggtaaaaa ttgtgtttct aatgtaaagc cagaatgtgg tttataaata
     3961 ttaaatggac tatcccactc atcaggctga ttaaaagtaa agaatacaac atgaggcata
     4021 tctgtatcga cctctaacat aaattcatga ttttcaacat acattttatg ttcaccaact
     4081 gtaaatgggt gatctagacc accaaaacgt gtcatttgtt ggcttaattg cgcatgttgt
     4141 gatgtaaaaa tatcactcaa ttttatcttg ttatcttcaa acacttcatg taaatctaga
     4201 atatcacctg taacaatatt tcgctcatct aatacataca tatctaattg attacttgaa
     4261 atgcgatgat tatcaacgac attattatct cgattcaaat tgaagtacac atgattcgta
     4321 ggactaaaca atgtgtcttc tgatgcaact gcttcgtatt caatcgacca ttggtgatcc
     4381 gcatcataaa tatgtgtaat cgtcacatcg atatcacctg ggaaatgatc atcagctgat
     4441 ttcaacaccg ttttaaatat cactttaatt tgagcaattt catttctaat ttcataatca
     4501 aataacttat tgtccaaacc atgacatcca ccatgtaaat gatgttcgcc gttgtttttt
     4561 tctaactgat attctttacc ttgcaactta aatttagcat tatcaattct accgctatat
     4621 cttcctatag aagcaccaaa tttaaaagga ttactatgat aaaattcatc cgcttcaaca
     4681 acatttccaa gaacaatatt attatcatga tatttccaag acactactct tgctccataa
     4741 ttcgtaaaaa taattttagt ttcatcatta tcaattttga ttaaatctac accttgtctt
     4801 tggtgctcaa cttcaactat catttttact tctcccttct aaccacaagt gttcaagctc
     4861 tgctgggcag caacattact aaaacaccta caatacaaat gattgcaccg ataacatcat
     4921 atttatctgg catttgttta tctacgacca tcgcaaaaat caaactcatg atgataaata
     4981 cgccaccata tgctgcatat actcttccga atgatggaaa tgattgaaat gtcgcaatga
     5041 caccatataa catgagtatc acaccgccta ttagcccaac aagtgaagac tgtccttccc
     5101 taagccacag ccaaatcagg tatcccccac ctatttcaca taagccagct aatataaata
     5161 taaaaatcgg atataacatg aaatcactcc atcacacatt tgctaccaat aatctatcgg
     5221 ctacatatca tttgtttaca tttcttctta cttcacattc ccattttaaa aagttcgttt
     5281 tcacattcat attgtacact tttttagaca ttattctata gctaaatata aaaaaataag
     5341 agtaacacgc tttcatcatc attttatatg ataaatgtgt gtcactctca tcaattttat
     5401 tttttaaata cacgtttcat tgaattaaat aagccacgtt caaatgtaag tactgaatct
     5461 ttatatgttt taattgcaat ccatatcaag acagctacca ttacaattga gattaatgaa
     5521 cttaagatga cttcatgtaa ttgaagccct gaagtttgag cgcgtacaac taattgaaat
     5581 ggcgccaaaa acggaatata acttgtgatt aaagcaagtc gcccatcagg attatttatc
     5641 gtgaatatgg cgatataaaa tgcaatcata ccaagtaatg tcagtggcat caaagattga
     5701 tttaaatctt ctattctaga tgttaatgat ccgagaatgg ctgcaagtaa tacatacgcc
     5761 ataattccaa caatactact tataattccg acaataataa tttgccaaga caattgattc
     5821 atttccacgt taaaaccttg tagcaagtct tttaagtcaa aggcaaaaat gcatatgact
     5881 gccatcaata caattaaaat aatctgagtc acggcaactg ctataatgcc taaaatttta
     5941 gcaaaaatat gttggattgg tgaaatactt gtaataatca tttcaatgac acgcgatgtt
     6001 ttctcactag caatttccat agctatttga gatgcataat ttaaaacaat aaagaacatt
     6061 agaaagataa tgccataagc taaagcatag ttaaaaatct tttgtccttc tgatacttta
     6121 tcgacttcat cattagaaat caccttatta tcaactttac tttgtgcttg taatttttgt
     6181 aagtcttctt tgttgatatt taattccccg gctaccatat ttgtttgaat agctgtaagt
     6241 agtgcttgta ctttttgtga atcttcatga cttactcgct tctcactaat gattgtccct
     6301 tgcaacgtgc gattttgatt caccttgata atataagctt tatcaagttt atgttttttc
     6361 acttcttttt cagcatcttc tatagaaact ttagtaaact tagcatcact atgaaatgta
     6421 ttcgcctgtt gcttgaaaac cttatagatt tgttcattcg gtgctgctac accaatttta
     6481 tctggaccat catcaaacat gttaataatc ttatcaatgt tagataggcc aatcattaag
     6541 gcagcaatga taatcataaa aattacaaat gatttagctt taattttttt gatatatgtc
     6601 aaagtaaatg tcgcccaaaa cttatgcatc cttgccacca accttctcaa tgaatatatc
     6661 ttgtaatgat ggttctacaa cttggaatcg tttaacataa ccttgatgtg ctacaacttg
     6721 ataaatatct ttggatacgt cttcattctc aatcgtcaac tgaagacctt gcttcatgtt
     6781 ttcactatga atgatgcctc taatgtgtgt taaatctggt agtgttgttt ctgactcaat
     6841 gacaactttc ttgttaccat tagatgcacg tacatgattg atatcaccag aaacaacaag
     6901 ttgaccttta tctaaaatac aaacatcatc acataattct tcaacatgtt ccatacggtg
     6961 agaactataa acgattgtac tgccccaatc atttaagtct ttaactgctt cttttaataa
     7021 ctcaacatta actgggtcta gaccactgaa aggctcatct aatattagta attctggttt
     7081 atgtaacata cttgctaaca gctgaatttt ttgttgattc ccttttgata gactatcaat
     7141 tcgtttttta cggttttcag taatatcaaa acgctcaagc caatacgata tttgctgttg
     7201 tatttctgtt tttgacattc cctttaaagt tgccaaatat ttcaattctt cttcaactgt
     7261 caatttccca tgtaaaccgc gttcttccgg taaataacca atacgattgt acattgtttt
     7321 atctagtttt ttaccgttat acgtaatgtg tccttcagtt ggttcactta agcctaaaat
     7381 catacgaaat gtcgttgttt tacctgcacc atttcttcct agaaaaccta acattttgcc
     7441 tgattctaac tctaatgaca tatcatttac tgccgtcatc ttaccaaaac gtttcgtaac
     7501 atgctcaatt acaagtccca tcttttgcct cctaaaacaa tatgtattta tcttaatata
     7561 acatttctat tctctataaa tacaatattt ttaaagtgaa tttattttta aaatttctga
     7621 aattgaaata tttaaatagt atcttctttg catgttaagt atcatgagcg ttagatatgc
     7681 ttttttccat gcctttattg ccttattggt aaaaaaaata tcccagcaac aatctgctga
     7741 gatatctata tcattacaat tgattatctg tttcttccgg tgttttcggt gtttcagatg
     7801 tccccgcttt tttcggtgtt tcagatgtca ctgattgatt tttaattgaa gatgggtatt
     7861 ttgggctttc gccatattta tttgccggct tactgtcaaa gcataggaat actatcataa
     7921 caattgttag gcctaaataa acaaaataaa gaagtactaa caaaatatta agacccatcg
     7981 gcattaaggt aaaatcactg tcataataac tatcgataat ctgtaatact atataaaata
     8041 taatactgaa tactgtcata atcattggaa ataacattgt tcttgatata tcgtgaaatc
     8101 ttcgaacgca caacgctaaa tttggaataa acgttgccaa actatagaca aaagtataca
     8161 cagatgtaag gataatcatc aatatactca taactattaa tgcttcgtta tctgccgcta
     8221 tagaaataaa gaatataaat aggcttatta ttagcacaca cacagctgga accataagta
     8281 ttaaatgcca caacatcata taccaatatt cactacgtct tgatctcccc ttaaaattaa
     8341 cataattttt ccaaaataat acgaatgatt tcataaaacc tacttgaggt aattgttcca
     8401 ttgtaatctc cctttcgtta atcatattta tatttttaat tattattacc gttataattt
     8461 acaagattca ttatcaagcc acttcatttc aatcgtcatt cactttggaa aatgaagttt
     8521 atatttcatc actagttaaa tgaaaacccg ctacaagtac gcatctatat ggagactcat
     8581 ttgaaagtca atgcttcgtt aa
//
LOCUS       DAMZPP010000118         8495 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMZPP010000118 DAMZPP010000000
VERSION     DAMZPP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.118"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            79..720
                     /locus_tag="POH73_002150"
     CDS             79..720
                     /locus_tag="POH73_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HDI6888079.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPVIVPTDTNYNLCSLPNNDLCI
                     DKIFEYKKRSKDKPLSLFIDKPEDWKLYGDNQNTEIVDKLVEIFWPGPLNIILKNKTS
                     YNYMLNNSDSIAIGCVQNKTMRRFISYINSPIAITSANISGTANDILITENEAIKHMG
                     ENVRYMLRSQNKTNYKTSSTIIKVTDNKIELLREGDIKFEEIKERLGTGIIYE"
     gene            713..1831
                     /locus_tag="POH73_002151"
     CDS             713..1831
                     /locus_tag="POH73_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888080.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLVQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVVSEIFKIIGLLLLLYQNQYLILVVAQILLG
                     LSYSMMTGVDTAIIKRNITNEKYVQNKSNSYMFLSLLISGIIGSYLYGINIKWPIIMT
                     GIFSILTIIIIRCTLVENRELNLIGETKGKIKKFLPEEKFWILHYSFLRALILGFFIG
                     FIPINIYNDLKLNNSQFISVLTCYTVMGYLSSRYLTKYLNYKFVSEICLVIFLIIYTY
                     QSFIAVTISMIFLGISSGLTRPQTINKLSSSSNLRVMLNYAETLYFIFNIAFLLMGGY
                     LYTIGTIQYLILFIALLIFIYLIIIFYFTRREQHETKN"
     gene            1815..2330
                     /locus_tag="POH73_002152"
     CDS             1815..2330
                     /locus_tag="POH73_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDI6888081.1"
                     /translation="MKLKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVTELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDKDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
     gene            2641..3075
                     /locus_tag="POH73_002153"
     CDS             2641..3075
                     /locus_tag="POH73_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDI6888082.1"
                     /translation="MKLKSFITVTLALGMIATTGATVASNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
     gene            complement(3332..3517)
                     /locus_tag="POH73_002154"
     CDS             complement(3332..3517)
                     /locus_tag="POH73_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDI6888083.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
     gene            3812..4753
                     /locus_tag="POH73_002155"
     CDS             3812..4753
                     /locus_tag="POH73_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDI6888084.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASVKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSEGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKLKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            4765..6009
                     /gene="fabF"
                     /locus_tag="POH73_002156"
     CDS             4765..6009
                     /gene="fabF"
                     /locus_tag="POH73_002156"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDI6888085.1"
                     /translation="MSQNIRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVSS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(6064..6435)
                     /locus_tag="POH73_002157"
     CDS             complement(6064..6435)
                     /locus_tag="POH73_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDI6888086.1"
                     /translation="MLKKWLGMALITPMLTFIIWVFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            6678..7604
                     /locus_tag="POH73_002158"
     CDS             6678..7604
                     /locus_tag="POH73_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6888087.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            7604..>8495
                     /locus_tag="POH73_002159"
     CDS             7604..>8495
                     /locus_tag="POH73_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6888088.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVQHRNLPA
                     KIPLLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIG"
BASE COUNT         3023 a         1129 c         1506 g         2837 t
ORIGIN      
        1 ttgtgaacaa gttttcacct gaagtagcac ctgaagatac agtggattta cttcagataa
       61 taaaaaggag gagtaaagat ggaaatttta aatgattctg taaaaagttt tgaaaaactt
      121 tatttacaat tacaagatgg ttttccagtg attgttccta ctgatactaa ttataattta
      181 tgtagtttac caaacaacga tctttgtatc gataaaatat ttgaatataa aaagcgatcg
      241 aaagataagc cattatcatt atttattgat aagccagagg attggaaatt gtatggagat
      301 aatcaaaata cggaaatagt tgataaacta gttgaaatat tttggcctgg accattaaat
      361 attattttaa aaaataaaac aagctataat tatatgctca ataattcaga tagtatagct
      421 ataggatgtg tacaaaataa aacgatgaga aggtttattt catatattaa ttcacctata
      481 gcaattactt cagcaaatat ctctggaact gccaatgata ttttaataac tgaaaatgaa
      541 gcgattaagc acatgggtga aaatgtaaga tatatgctaa gaagtcaaaa taaaactaac
      601 tataaaacgt ctagtacgat tattaaagtg acagataata aaattgaatt attaagagaa
      661 ggagatataa agtttgaaga aataaaagaa agactaggta caggtattat ttatgaataa
      721 gttaatactt gggatttatt tataccgaat tttttcacga gcgtactttt atttaccttt
      781 tttattaatt tactttttgg ttcaaggtta ttccataata caattagaaa tattaatggc
      841 gtcttatggc attgcagcat ttttattctc tctatacaaa gagaagtgtt ttaaaatttg
      901 taacttaaaa gattctaata aattagttgt tagtgaaata ttcaaaatca tcggtttatt
      961 gttgttatta tatcaaaatc aatatttaat tttagtagtg gcacaaatat tattagggtt
     1021 aagttactca atgatgacgg gtgttgatac agcaataatt aaaagaaata taacaaatga
     1081 gaaatacgta caaaataagt caaatagcta catgttccta tcattattaa tttcagggat
     1141 tataggtagt tatctttatg gaataaatat taaatggcct ataataatga ctggtatatt
     1201 ttcaattcta acaattataa ttattcgctg cacattagtt gaaaataggg aattaaattt
     1261 aataggagaa acaaagggaa agataaagaa atttctaccg gaagagaagt tttggatatt
     1321 gcattattct tttttaagag cgttaatatt aggatttttt ataggattta ttccaattaa
     1381 tatatataat gatttaaaac tgaataattc acaatttatt tcagtattaa cttgctacac
     1441 agttatgggt tatttatctt cacgttattt aaccaaatac ttgaactata agtttgtgtc
     1501 agaaatttgt ttagtaatat ttttaataat atatacatat caaagtttca tagcagttac
     1561 tatttctatg atatttttag gtatttcttc agggttaact cgtccacaaa ctataaataa
     1621 actttctagc agtagtaact taagagtgat gcttaattat gcagaaacgt tatattttat
     1681 ttttaatatc gcatttttac ttatgggtgg ttacttatat acaataggaa ctattcaata
     1741 cttaatatta tttattgcat tattaatttt tatatattta ataataatat tttattttac
     1801 aaggagagag caacatgaaa ctaaaaactg aatttaaagg gaacaatata ccatatgaat
     1861 acgcagcagg tgcagatgtg agtgattcta ttaacgggaa tccaattaaa tcatttccat
     1921 ttgaagtaac tgaattaccg gaagggacta aatatcttgc ttggtcttta attgactatg
     1981 atgcaatccc agtatgtggc tttgcttgga ttcattggag tgtagctaat gtaagtgtta
     2041 gtggcaattc aatttctatt aaagcagatt tatcaagaac aaagggcgac tatgtacaag
     2101 gtaaaaatag cttcactagt ggtttgttag ctgaagattt ttcagaaata gaaaatcact
     2161 atgtaggacc aacaccacct gataaagatc atcaatatga attaacagtt tatgcgttag
     2221 atcattcttt aaatttgaag aatgggttct acttgaatga atttttaaaa gaagtaaatc
     2281 aacataaaat agatcaaaca agtattaacc ttataggaag aaaaatttaa tattaaatat
     2341 ctcatcaata taaaattgtt caattaaaag tacgaagaaa caagggattt aatttatata
     2401 ttaggtacgg cgttcgctat aatgcaatga agtaattaaa tttaagaaat gtaaacttag
     2461 ttattgtaat gtgaatttat ttgaaaaaat agaaagtatt aacaattata gcttttacat
     2521 taattaaaat ttatttttaa aaacaagtaa acaatttaca tacttataat ttttgaaact
     2581 tttagatttg tgttatattg attttgtaag atactttaac tcacaaagga gagagattat
     2641 atgaaattaa aatcatttat aactgtaact ttggcactgg gcatgatcgc aacgactggc
     2701 gctactgtgg caagtaatga ggtatctgca gcagaaaagg acaaactacc ggcaactcaa
     2761 aaagctaaag aaatgcaaaa tgttccatat acaattgcag tagatggcat tatggctttc
     2821 aatcaatctt acttaaattt accaaaagat agccaattat catatttaga tttagggaat
     2881 aaagttaaag ctttattata tgatgaacgt ggtgtaacac ctgagaagat tcgaaatgca
     2941 aaatctgccg tttacacgat tacttggaaa gatggcagta aaaaagaagt ggatttaaag
     3001 aaagatagtt atacagcaaa cttgtttgat tcaaattcaa ttaagcaaat tgatattaat
     3061 gtaaaaacta aataaacact taaaattatc aaattcaaca tgaagcattt aattttacag
     3121 tgatgattat aatataattg ccttgataca aagattactc gtaaatgaca tctttgtatt
     3181 aaggcttttt ctaaatttaa aagtgatggg ttagaggtca ttgagtttta aaatattcca
     3241 aatacaaaac attaatggcc aaaaataaaa gccgccttta tctgggcagc ttcaataata
     3301 agaaagacat atttcatttt atactaaata gttattgtga tgaatctttc ggcggtttaa
     3361 ttactgcagc gaaaattgct gtgaaaatcg tgaacaatac tgccatgata attggattca
     3421 ctacatttaa gctgtctcca cctactaggc tattaagtac aaagttaacc atttgcatta
     3481 ataataatgc ccaaaagaat gttacgaggt gtttcatgtc attctacctc cactttaatt
     3541 atatatattt tattttaagt gaaagttaga aatttgtata gtaacatctc atatattttg
     3601 accatattat acagtttaaa taaatgattt tatctgaatg gctattctaa attaagcgca
     3661 ttaaaatcaa tttcatactg aaatttgacg ataataaagc attaaaattt tattaactag
     3721 tcaatattcc tacctctgac ttgagtttaa aaagtaatct atgttaaatt aatacctggt
     3781 attaaaaatt ttattaagaa ggtgttcaac tatgaacgtg ggtattaaag gttttggtgc
     3841 atatgcacca gaaaagatta ttgacaatgc ctattttgag caatttttag atacatctga
     3901 tgaatggatt tctaagatga ctggaattaa agaaagacat tgggcagatg acgatcaaga
     3961 tacttcagat ttagcatatg aagcaagtgt aaaagcaatc gctgacgctg gtattcagcc
     4021 cgaagatata gatatgataa ttgttgccac agcaactgga gatatgccat ttccaactgt
     4081 cgcaaatatg ttacaagaac gtttagggac gggcaaagtt gcctctatgg atcaacttgc
     4141 agcatgttct ggatttatgt attcaatgat tacagctaaa caatatgttc aatctggaga
     4201 ttatcataat attttagttg tcggtgcaga taaattatct aaaataacag atttaactga
     4261 ccgttctact gcagttctat ttggagatgg tgcaggtgcg gttatcatcg gtgaagtttc
     4321 agaaggcaga ggtattataa gttatgaaat gggttctgat ggcacaggtg gtaaacattt
     4381 atatttagat aaagatactg gtaaactgaa aatgaatggt cgagaagtat ttaaatttgc
     4441 tgttagaatt atgggtgatg catcaacacg tgtagttgaa aaagcgaatt taacatcaga
     4501 tgatatagat ttatttattc ctcatcaagc taatattaga attatggaat cagctagaga
     4561 acgcttaggt atttcaaaag acaaaatgag tgtttctgta aataaatatg gaaatacttc
     4621 agctgcgtca atacctttaa gtatcgatca agaattaaaa aatggtaaac tcaaagatga
     4681 tgatacaatt gttcttgtcg gattcggtgg cggcctaact tggggcgcaa tgacaataaa
     4741 atggggaaaa taggaggata acgaatgagt caaaatataa gagtagttat tacaggtatg
     4801 ggagcccttt ctccaatcgg taatgatgtc aaaacaacat gggagaatgc tctaaaaggc
     4861 gtaaatggta tcgataaaat tacacgtatc gatactgaac cttatagcgt tcacttagca
     4921 ggagaactta aaaactttaa tattgaagat catatcgaca aaaaagaagc gcgtcgtatg
     4981 gatagattta ctcaatatgc aattgtagca gctagagagg ctgttaaaga tgcgcaatta
     5041 gatatcaatg aaaatactgc agatcgaatc ggtgtatgga ttggttctgg tatcggtgga
     5101 atggaaacat ttgaaattgc acataaacaa ttaatggata aaggcccaag acgtgtgagt
     5161 ccatttttcg taccaatgtt aattcctgat atggcaactg gacaagtatc aattgactta
     5221 ggtgcaaaag gaccaaatgg tgcaacagtt acagcatgtg caacaggtac taactctatc
     5281 ggtgaagcat ttaaaattgt gcaacgcggt gatgcagatg caatgattac tggcggtacg
     5341 gaagctccaa ttactcatat ggcaattgca ggtttcagtg caagtcgagc gctttctaca
     5401 aatgatgaca ttgaaacagc atgtcgtcca ttccaagaag gtagagacgg ttttgttatg
     5461 ggtgaaggtg ctggtatttt agtaatcgaa tctttagaat cagcacaagc tcgaggtgca
     5521 aatatttatg ctgagatagt tggctatggt actacaggtg atgcttatca tattacagcg
     5581 ccagctccag aaggtgaagg cggttctaga gcaatgcaag cagctatgga tgatgctggt
     5641 attgaaccta aagatgtaca atacttaaat gcccatggta caagtactcc tgttggtgac
     5701 ttaaatgaag ttaaagctat taaaaataca tttggtgaag cagctaaaca cttaaaagtt
     5761 agctcaacaa aatcaatgac tggtcactta cttggtgcaa caggtggaat tgaagcaatc
     5821 ttctcagcgc tttcaattaa agactctaaa gtcgcaccta caattcatgc ggtaacacca
     5881 gacccagaat gtgatttgga tattgttcca aatgaagcgc aagaccttga tattacttat
     5941 gcaatgagta atagcttagg attcggtgga cataacgcag tattagtatt caagaaattt
     6001 gaagcataat tataaaaatc ttcagtaacg ttgttttagt tactgaagat tttttctgtt
     6061 tctttatact aagatgagcg acagcacaat cgtcataata aaatataaaa tatttattaa
     6121 taataagggt attatccatg tagaaacaaa gtaatgctct ttttttacct cttgtgggtt
     6181 gaaaaatgga tcatcagaga tagacttctt ctttttcgaa gatgacattt gatactttaa
     6241 tcttctaaaa ccataacttg tcgcatcaaa aatgccttct tgtacaagta aaatcaaaaa
     6301 aatgctaata aaaataatta atgaaacata aaacaatata tttaaatatg taatgatagt
     6361 atggctatta aaaacccata taataaacgt tagcattggg gttattagtg ccattccaag
     6421 ccattttttc aacatttgat cactcccact tatagaaaac tcttacgcat agtttacatt
     6481 aaaatgagac attgaggaat gattttttaa tttcttcagc tttattgaaa ttctaaaatc
     6541 aatcattctt cattagttta aagcaaaaaa atattgatat atagtaaata ttgtatatat
     6601 aatattagtt aagatttcag aaaattttga agggaatgga aatttagaaa tcggaatttg
     6661 ttagaggagg ggattagatg gggaaatata tttttaaacg atttatttat atgcttattt
     6721 ctttatttat tattattaca attacatttt tcttaatgaa attaatgcca ggttcaccat
     6781 ttaacgatgc taaattaaat gctgaacaaa aagaaatttt aaatgaaaaa tatggattaa
     6841 atgatcctgt agcaacgcag tatttacatt atttaaaaaa tgttgttaca ggggattttg
     6901 gtaattcatt ccaatatcat aatcaacctg tgtgggattt gattaaaccg agactattac
     6961 cttcttttga aatgggtctt acagcaatgt tcatcggtgt gatactgggt cttattttag
     7021 gtgttgcagc agctactaaa caaaattctt gggttgatta tacaactaca gttatttcag
     7081 ttattgcagt atctgtacca tcttttgtac ttgctgtact tttacaatac gtatttgcag
     7141 ttaaattaag atggttccca gtagctggat gggaaggttt ttcgaccgcg gtattaccgt
     7201 cacttgcatt atctgcagct gttttagcaa ctgtcgccag atacataaga gcagagatga
     7261 tagaggtatt aagttcagac tacattttat tagcgagagc taaaggtaat tcgacaatgc
     7321 gtgtactttt tggacatgca cttagaaatg ctttaattcc aattattaca attatcgttc
     7381 ccatgttagc aagtatttta acaggcactt taacaattga aaatattttt ggagttcctg
     7441 gattagggga tcaattcgta cgttcaatta caacaaatga tttctcagta atcatggcaa
     7501 tcacactatt atttagcaca ctgtttatcg tttctatttt tattgtagat attttgtacg
     7561 gtgtgataga tccacgaatt cgtgttcaag gaggtaaaaa ataatggctg aaaataaaaa
     7621 caatttgtcg attaacgacg atcattctaa tgcagctatg acgcatacct ctgacgctat
     7681 cgcatcatct gattttatta ttagagaatt agatttgaat caggaacctg aaatgcaacg
     7741 agaaagcaaa aacttttggc aagatgcttg ggctcagtta aaacgaaata agttagctgt
     7801 tgtcggtatg ataggtttaa ttatcattgt aatatttgct tttatcggtc cagttataaa
     7861 taaacatgat tatgctgaac aaaatgtaca acatagaaat cttccggcaa aaataccttt
     7921 attagacaaa gttccatttt taccttttga tggtaaagat gcagatggca aggatgctta
     7981 taaagcagca aatgctaaag aaaattattg gtttggtact gatcagttgg gtcgagattt
     8041 atggacaaga acatggaaag gtgctcaaat ttcattgttt atcggtgttg ttgcagcgat
     8101 gttagatatt tttattggcg ttgtatatgg tgcgatttct ggattcttcg gtggacgtgt
     8161 cgatacgatt atgcaacgta tacttgaagt catagcatct attccgaatt taattgtcgt
     8221 aattttattt gtattaattt ttgaaccatc catttggaca attatattgg ctatgtctat
     8281 cacaggctgg ttaggcatga gcagagttgt acgtggagaa tttttaaaat tgaaaaatca
     8341 agagtttgtc atggcttcga aaacattggg ggcttctaaa ttcaaattga tatttaagca
     8401 tattttacct aatacgttag gtgctatcgt ggttacatca atgtttacag tacctagtgc
     8461 tattttcttc gaagcatttt taagtttcat tggta
//
LOCUS       DAMZPP010000119         8433 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMZPP010000119 DAMZPP010000000
VERSION     DAMZPP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.119"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..1808)
                     /gene="farE"
                     /locus_tag="POH73_002160"
     CDS             complement(<1..1808)
                     /gene="farE"
                     /locus_tag="POH73_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HDI6888089.1"
                     /translation="MAKFLYKMGTFIAKYKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPSITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQINKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAVGTAG
                     SAVIFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVIFAVFSALTLLPALISIFHKHI
                     KVNKLQSNFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYNKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFATVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTFV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMD"
     gene            1953..2501
                     /gene="farR"
                     /locus_tag="POH73_002161"
     CDS             1953..2501
                     /gene="farR"
                     /locus_tag="POH73_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HDI6888090.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHKVINIKIK"
     gene            complement(2578..2745)
                     /locus_tag="POH73_002162"
     CDS             complement(2578..2745)
                     /locus_tag="POH73_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888091.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFMFFFIVYVLYISYF
                     NSDKHDKNKKR"
     gene            complement(2857..4401)
                     /gene="pruA"
                     /locus_tag="POH73_002163"
     CDS             complement(2857..4401)
                     /gene="pruA"
                     /locus_tag="POH73_002163"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HDI6888092.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(4591..5190)
                     /locus_tag="POH73_002164"
     CDS             complement(4591..5190)
                     /locus_tag="POH73_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDI6888093.1"
                     /translation="MTEKEKMLAEKWYDANFDQDLINERARAKDICFELNHTKPSDKN
                     KRKELIDELFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPINIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNEVPSEALNDETLN"
     gene            5429..5620
                     /gene="cwrA"
                     /locus_tag="POH73_002165"
     CDS             5429..5620
                     /gene="cwrA"
                     /locus_tag="POH73_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HDI6888094.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            5860..8268
                     /locus_tag="POH73_002166"
     CDS             5860..8268
                     /locus_tag="POH73_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDI6888095.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNRDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGCPVVT
                     DYHGDDQTLQLLATAEKDSEHPLAEAIVNYAKEKQLTLTETTTFKAVPGHGIEATIDH
                     HHILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQITKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
BASE COUNT         2717 a         1580 c         1356 g         2780 t
ORIGIN      
        1 taatccattg ctaagccgaa tagtatacca attgtaataa ccggcaagaa ggctagcatt
       61 ggtcctgtcg tttctatacc gaataatcct ttcataaagc catcttgcat cacaaaggtt
      121 gtaaaaccta gtgttgccat taatgacaag acaaatccta aaactgcttt taacggtatt
      181 aagattgaac ggaagacaat cattaataag aaaaatgcca atacaacaat tactgttgca
      241 aataaaggta tcgcctcatt taacttttta gacatatcaa tattaatgac actttgtcct
      301 gaaatttccg ttttaaatcc atatttatct tgcgcatctt tattataatc tcgtaaatca
      361 tgcactaagt catttgtact ttctgcatta ggcccttgct taggtatcac gaccatcaaa
      421 gcgtaatcat tatctttact catttgtggc ggcgtaacaa tatccacatt tttcttatct
      481 ttaatatctt tatacacaga ttgtaaatct tgttgtaatc cttgtggatc atctttttta
      541 tctttaacgt taatcagcat cggtatttgg ccattaaatc cttcgccaaa cttatctgaa
      601 ataatatcgt atgccttttt ctgagtagaa tctgctggct ttacgccatc atccggaatg
      661 ccaagacgca tatgactaac tggaatagcc gctgcaacta atataattaa acctaataat
      721 actgctgcaa gtgcattacc agtaataaat ttagaccacg gcgtatcaat atctttttta
      781 aaattagact gcaatttgtt cactttaatg tgtttatgga aaatactaat taacgcaggt
      841 aataaagtca gtgcactgaa tacagcaaat ataacactaa ttgccgaagc aaaccccatc
      901 actgctaaga aatctatacc aactagcgat aaaccgcata cggcaatcac aactgtcaca
      961 ccagcgaaga tgactgcgct acctgctgtt cccactgcaa gaccaatgcc tttaatgtaa
     1021 tctgtttcag ttttcataac ttgtcgatat ctgaataaaa taaataatgc ataatcgata
     1081 ccaactgcta gtccaatcat tacggctaat gtcagtgtga catttggtat atcgaatgca
     1141 taagttaaca aactgataat acctacacca gaagctaaac caattaatgc acttataatt
     1201 ggtaatcctg cagcaataac tgaaccgaat gtgattaaca gtacaacaaa tgcaacaata
     1261 ataccaacta gttcagaatt accgcctact tctgtagatg tcattcctgt accagttaac
     1321 tctgtttgta cattatgatt gtcacgtaag tctttcaaat gacttttaac attatctcta
     1381 gagccatctt ttaaagatgt ttgactaacg tcatatgtga tatctgcaaa tgcagttgtt
     1441 ttatctttat taatttgctt attttcataa ggatctgata ttttatcaat gtgcttgtca
     1501 tcttttttaa tatcatctaa cgttttctta atatctttag taatactcgg ttgcacaata
     1561 ccatcatctt tagtcgtctt aaagacaaca cgtatttgtg ccttttcact atcttgatta
     1621 aaatgttttt caatcttttt attcgtatct aacgactcta gtcctgtcat tttaatatca
     1681 ttgtcaaatt tcggtgcatt tgtagcaagt ggtatcaata ttgcagctac aatcactatc
     1741 catgcaatga ccgcggacca tttatatttt gcgatgaatg tccccatctt atataaaaat
     1801 tttgccaaag tatattgcct ccttttaaaa tcaacgttgt agtttaacta tacagtgtag
     1861 attattgttt gattatagta tctatcaccg acctcttaaa gaatcaattg gaaaattttg
     1921 tatattaaac tacacacaaa ggagaaatgt agatgaaaga gactgattta cgagttataa
     1981 agacaaaaaa agcattgtcg agtagcttgc tacaattgtt agaacagcaa ttattccaaa
     2041 cgattactgt caatcaaatt tgcgacaacg cactcgtaca ccgcacaaca ttttataaac
     2101 acttttatga taaatatgat cttctagagt atttgttcaa tcaattgact aaagactact
     2161 ttgctagaga tatcagtgac cgtcttaatc atccattcca aacgatgagt gatacgatta
     2221 ataataaaga ggatttgaga gaaatcgcag aattccaaga agaagacgct gaatttaata
     2281 aagtattaaa aaatgtctgc attaaaatta tgcataacga tatcaaaaat aatagagacc
     2341 gtatcgatat tgacagcgac atcccagata atctcatatt ttatatttat gactcgttga
     2401 ttgaaggttt tatacattgg ataaaagatg aaaaaattga ctggcctggc gaagatattg
     2461 ataacatttt ccataaggtc attaatatta agattaaata gtaaattaga ggctcataag
     2521 cgttaccaac attcataata aaaacgatag tgtacacgtt aatgaattcg tgtactacta
     2581 tcgtttttta tttttatcgt gcttatcgct attaaaataa ctgatataca acacataaac
     2641 tatgaagaaa aacataaatc cgctatctaa atgactttga ctcagttgtt taaatgacca
     2701 aattgctaat acaattccca ttattattga aataacgtat ctcacattct tatacctaga
     2761 atccttttct aaaaatatgg ttgctattac ttaattttta aagttataaa taaaaagagc
     2821 caaccgcaat ggatggccct tgttcattat gaagcattag aacatttctg aaacaacctt
     2881 ttgttctaag aagtgtaata agtagtctgg gctacctgtt ttagcgtccg tacctgacat
     2941 tttgaaacca ccaaatggat ggtatccaac aactgctgaa gtacagcctc tgttaaggta
     3001 taaattacct acatcaaatt cgtttaccgc tttaatccaa tgctcgcgat tatttgtaat
     3061 cactgcacca gttaaaccgt aatctgtatc atttgcaacc tcaattgctt catcaaaatc
     3121 gttaactttc acaaagccaa caactggacc aaaaatttct tcttgcatga ttctatcttt
     3181 agatttaagt cctgaaatga ttgttggttc tacaaagtaa ccttttgaat catcagtgcc
     3241 gccaccttgt tctaatttac cttcttcttt accaatttca atataatttt taatcttatc
     3301 aaattgtttt ttattaataa ctgggcccat atacgtattg tctacagtat tacccaacgt
     3361 taattctttt gttagtttaa ttgatttttc taatacttcg tcataaacgt ctttatgcac
     3421 aattgcacgt gaacatgctg aacatttttg accagagaaa ccaaacgctg acgttacaat
     3481 agcttctgct gccatatctg tatcaatatt ttcatcaact acaatggcat ctttaccgcc
     3541 catttcagcg ataacacgtt tcaagaagtt ttgaccttct tgaacaacgg cactacgttc
     3601 ataaattcta gtacctgtcg cacgtgatcc tgtaaatgta acaaaatgcg tatctttatg
     3661 atcaactaag taatcaccaa tttctttcgg atcaccagga acaaagttaa ctacgccttt
     3721 tggtaatcct gcttcttcta aaatttccat taatttataa gcgatataag gtgtatcctc
     3781 agcaggtttc aataacactg tattacctgc cacaactggt gctaaagttg taccagccat
     3841 aatcgcaaac gggaagttcc acggtggaat tgtaacacct gtaccaattg atttatagaa
     3901 atatttattg tgttcacctt cacgatcaag tactggcttg ccttgagcca agtccatcat
     3961 tgaacgtgca tagtattcaa taaaatcaat accttcagct gcatcaccaa ctgcttcatc
     4021 ccatggctta cctgcttcat aaaccataat tgctgcaatt tccgcttttc gacgacgaat
     4081 aattgccgaa acacgtaaca taagctctgc acgatcattt gctgaccatg ttttccaaga
     4141 tttataagct tcgttcgctg ctttaaatgc atcttcaaca tcttgttttg ttgcctttga
     4201 tgcatttgca atcacttgtg atgtgtctgc aggattgatt gatttaattt tgtcatcttt
     4261 gaaaatcttc tctccattaa tcactaatgg tatgtcttga cctaattctt tttccacgtc
     4321 tttcaatgct ttcttaaaca tatccacatt ttcttggact gaaaaatcgt aaccaggttc
     4381 atttttaaat tctactacca tgtacactta ccccctataa attttgaaag tggtttaacc
     4441 ctttgattta atgatataac atcatttaaa ctcattttac tatgagtaag gttggttttg
     4501 caatcgcttt catttttatg ctttatccct tattctcaag tattttgaaa ttgattggtt
     4561 actttttaaa atttagaagt gtcatgacaa ctaatttagt gtttcatcgt tcaatgcttc
     4621 tgatggtacc tcattatcaa ttttacgaac gactttacaa gggtttccaa ccgctaaact
     4681 gtgtggcgga atatctttgg tgacaacact accagcacca atcacactgc cttctccaat
     4741 cgtcactccc ggaagcacgg ctacatgtcc gccaaaccaa gtattactgc caatattaat
     4801 tggtcctgct ttttcaaatc cttcatttct atgatgaaaa ttaagtggat gtgttgctgt
     4861 gtagaatcca caattaggtc ctataaaaac attatcgcca attgtaatct gtccaccatc
     4921 cataaaataa caattggtgt ttacatagac attttttcct agtttaacgt tccaaccata
     4981 atctgtatca aaaggaatcg aaatacttac attgtctgtt gttgtttgaa ataattcatc
     5041 gattaattcc tttcttttat ttttgtcact cggctttgta tgatttaatt caaagcaaat
     5101 atctttcgct cgtgcacgtt cattgattaa gtcttgatca aagtttgcat cgtaccattt
     5161 ttctgctaac attttttctt tttcagtcat tacacctttc aactcctaat aacttattta
     5221 cttgtttaat agttaatcaa ataaacattc gcctatgcaa ctaataagct ataacattat
     5281 gaaatcataa ccttatcact cttatctata ccattctcgc atcaaatact gctaaagtag
     5341 tagataaatt caatactaca gacgcattca ttttttaatc tattaacgta aaatgtgagt
     5401 aagagaaata taaaggagta tgatagcgat gagaatatta attacaggca cagttgctat
     5461 cttaatcatt ctaggtttag ttaaaacgat acaagattac gaaatgacaa acgacacaag
     5521 tcgtcagttg tcagacaaca aagatgacga taaagtcatc catcttaata atttcaaaaa
     5581 tttacatgcg aaagaattta acccatctga tttcttttaa gtcacctaag aattacaaat
     5641 ccagaagtca tttaagtttt acctttcatt catacatcct ttaatattaa ttacgacttc
     5701 ttttatatag atgctaaata gagagattgt tgtgcaatgt ttgcacggca atctctcttt
     5761 ttctttttaa aattggtaaa agtaaaacgc aacgattgac ttatatacct atagggggta
     5821 cattagacgt gtaacaatga atcacaggga ggcaataatg tggctaatac gaaaaaaaca
     5881 acattagata tcactggtat gacttgtgcc gcatgttcaa atcgtatcga aaagaaactg
     5941 aataaacttg atgacgttaa tgcccaagtg aatttaacta cagagaaagc aactgttgag
     6001 tataaccctg atcaacatga tgtccaagaa tttattaata cgattcaaca tttaggttac
     6061 ggtgtcgctg tagaaactgt cgaattagac attacaggca tgacttgtgc tgcatgctca
     6121 agccgtattg aaaaagtgtt aaataaaatg gacggcgttc aaaatgcaac ggtcaattta
     6181 acaacggagc aagctaaagt tgactattat cctgaagaaa cagatgctga taaacttgtc
     6241 actcgcattc aaaaattagg ttatgacgca tctattaaag ataataatag agatcaaacg
     6301 tcacgcaaag ctgaagcgct acaacataaa ttgattaagc ttatcatatc agcagtatta
     6361 tctttaccgc tattaatgtt aatgtttgta catcttttca atatgcatat accagcacta
     6421 tttacgaatc catggttcca atttatttta gctacacctg tacaatttat tattggatgg
     6481 caattttatg taggtgctta taaaaattta agaaatggtg gcgccaatat ggatgtactt
     6541 gttgctgttg gtacaagtgc agcatatttt tacagtattt atgaaatggt tcgttggcta
     6601 aatggctcaa caacacaacc gcatttatac tttgaaacaa gcgccgtact aattacctta
     6661 atcttattcg gtaagtattt agaagctaga gcgaagtctc aaacaaccaa tgcgcttggc
     6721 gaattattaa gtttacaagc taaagaagca cgcattttaa aagacggtaa tgaagtgatg
     6781 attcctctaa atgaggtaca tgttggagat acacttatcg ttaaaccagg tgaaaagata
     6841 cctgttgatg gcaaaattat taaaggtatg actgccatcg acgaatctat gttaacaggt
     6901 gaatctatcc ctgttgagaa gaatgttgat gatactgtaa ttggttcaac gatgaacaaa
     6961 aacggtacta ttactatgac agcaacaaaa gttggcgggg acactgcatt ggcaaatatt
     7021 attaaagttg tcgaagaagc tcaaagttct aaagcgccga ttcagcgatt ggcagatatt
     7081 atttctggtt atttcgttcc tatcgttgtt ggtatcgcac tattaacatt tatcgtgtgg
     7141 attactttag ttacaccagg tacatttgaa cctgcacttg ttgcgagtat ttccgttctc
     7201 gtcattgctt gtccatgcgc attaggactt gctacaccaa cttctattat ggtaggtact
     7261 ggtcgcgctg ctgaaaatgg tattttattt aaaggtggcg agtttgttga acgcacacat
     7321 caaattgata ccatcgtttt agataagacg ggtaccatta caaatggttg tccagtcgtg
     7381 acagattatc atggtgacga tcaaacgcta caactacttg ctactgctga aaaagattct
     7441 gaacacccat tggcagaagc cattgtcaat tatgcaaaag aaaagcaatt aacattaact
     7501 gagacaacaa catttaaagc agtacctggc catggtattg aagcaacgat tgatcatcac
     7561 catatattgg ttggtaaccg taaattaatg gctgacaatg atattagctt gcctaagcat
     7621 atttctgatg atttaacaca ttatgaacga gatggtaaaa ctgctatgct cattgctgtt
     7681 aattattcat taactggtat catcgcagtg gcagatactg taaaagatca tgccaaagat
     7741 gctataaaac aattgcatga tatgggcatt gaagttgcca tgttaactgg cgataataaa
     7801 aacactgctc aagccattgc aaagcaagta ggcatagata ctgttattgc agatatttta
     7861 ccagaagaaa aagctgcaca aataacgaaa ctacagcaac aaggtaagaa ggttgcgatg
     7921 gttggtgacg gtgtaaatga tgcacctgca ttagttaaag ctgatatcgg tatcgccatt
     7981 ggtacaggta cagaagttgc tattgaagcg gctgatatta ctattcttgg tggcgacttg
     8041 atgcttattc ctaaagccat ttatgcaagt aaagcaacca ttcgtaatat tcgtcaaaat
     8101 ctattttggg cattcggcta taatattgcc ggtatcccta tagctgcatt aggcttactt
     8161 gcgccatggg ttgctggtgc tgcaatggca ctaagttcag taagtgttgt cacaaacgca
     8221 cttagattga aaaagatgcg attagaacca cgccgtaaag atgcctagat tccttaataa
     8281 tgaaggattc gttggtgatt ctgagatagg ctagtgattg gctctataat gtcgcggttt
     8341 acagttggat cttcgctcca actgcatata tagttacact tttcgcttgg cgaattagtg
     8401 tatcttggga gtgggacaga aatgatattt tcg
//
LOCUS       DAMZPP010000120         8422 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMZPP010000120 DAMZPP010000000
VERSION     DAMZPP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8422
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.120"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            460..633
                     /locus_tag="POH73_002167"
     CDS             460..633
                     /locus_tag="POH73_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888096.1"
                     /translation="MKKFFMFFVCLLTVFLSFNFVNSILFDGSFVFLESFIYSLVFTL
                     VFLGLEGDKEKKK"
     gene            complement(815..1138)
                     /locus_tag="POH73_002168"
     CDS             complement(815..1138)
                     /locus_tag="POH73_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDI6888097.1"
                     /translation="MEFYQMPMFNKVLMNHLRLEKYQDLMLISQKDFEVGNSIYLNIL
                     VKDIEEKSQNISQKFIVSQLEEQPWNAKEMTIKDPDGNLITLTQSNISDTDFEKLMKK
                     TSKDY"
     gene            1367..1459
                     /locus_tag="POH73_002169"
     CDS             1367..1459
                     /locus_tag="POH73_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDI6888098.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(1761..2582)
                     /locus_tag="POH73_002170"
     CDS             complement(1761..2582)
                     /locus_tag="POH73_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002477850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6888099.1"
                     /translation="MDSLKVLTEVVKTQSFTKAAENLYTSQPSISRDIKRLELEYDVK
                     IFEFRHSKMSLTSDGEKLFQYALQRERLEQNLRHDLKMQPDIISGDLTIGSSYTYGEY
                     RLSQHLADLAKNYPELYIHVYLNNSDTVLQHIKNNTIDLGIIEKEVQSNIIHSRRIAQ
                     DEIVLIRKKSSSSNKSICFIRERGSGTRVYQENALSQMTSSSYLVEINNTNLIKNMVH
                     ADKGFSIVSKSTLTPYDLEKLEVTNLDIKRYFYLVIHKDKYIDTKLNEVIKNLIS"
     gene            2707..3702
                     /locus_tag="POH73_002171"
     CDS             2707..3702
                     /locus_tag="POH73_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDI6888100.1"
                     /translation="MKSLKSKAFIYGLLFTFIIAVISLLGSKLPFLDKIGALTIAILI
                     AILYRHFKGYPEAYRSGIAFSSKRLLKLAIILYGLKLNIYDVIGKGSDLVLIDIGVIL
                     FSIGLMLLLNKYIKGDKNLILLLGIGTGVCGAAAIAAVSPILKSREKDSAISIGIVAL
                     IGTIFSLAYTVIYSIFTISPEVFGVWSGTSLHEIAHVILASDFSGQEALSMGLLGKLG
                     RVFLLIPLSIILILIMRKKSHSENEKKRIDVPYFLLGFVMMALFHTYVSLPHMVMQII
                     DNITTICLLMAMVALGLNVSFKDLKDRAFKPLIAVIIVSICLSTVTFIVAKYFYS"
     gene            complement(4037..4612)
                     /locus_tag="POH73_002172"
     CDS             complement(4037..4612)
                     /locus_tag="POH73_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6888101.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNAYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLKLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEDEKSDK"
     gene            4879..6924
                     /gene="copB"
                     /locus_tag="POH73_002173"
     CDS             4879..6924
                     /gene="copB"
                     /locus_tag="POH73_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase CopB"
                     /protein_id="HDI6888102.1"
                     /translation="MSNNKKHQNHEHHSHQEHENHTSHGNHEHHHHGNFKSKFFISLI
                     FAIPIIILSPMMGVKLPFQISFTGSDWIVLILATILFFYGGKPFLSGAKDEISTKKPG
                     MMTLVALGISVAYIYSLYAFYMNNFSGSSTHTMDFFWELATLILIMLLGHWIEMNAVG
                     NAGNALKKMAELLPNTAVKLIDNNQREEVKISDIHIDDIVEVRAGESIPTDGIIVRGE
                     TSIDESLVTGESKKVHKTHNDDVIGGSINGSGTVQVKVTATGENGYLSQVMGLVNQAQ
                     NDKSKAELLSDKVAGYLFYFAVSIGLISFIVWMLIQNNVDFALERLVTVLVIACPHAL
                     GLAIPLVTARSTSIGAHNGLIIKNRESVEIAQHIDYIMMDKTGTLTEGNFSVNHYESF
                     TDELNNEEILSLFASLESNSNHPLATGIVDFAKGKNISYATPQEVNNIPGVGLEGTVD
                     NKKLKIVNVSYLDKSNFDYNKEQFTNLAQQGNSISYLIHDRQVIGIIAQGDKIKESSK
                     QMVSDLLSRNITPVMLTGDNKEVAQTVAEELGISDVHAQLMPEDKESIIQDYQSNGSK
                     IMMVGDGINDAPSLIRADIGMAIGAGTDVAIESGDVILVKSNPSDIINFLSLSKNTMK
                     KMVQNLWWGAGYNVIAVPLAAGILASIGLILSPAVGAILMSLSTIIVAINAFTLKLK"
     gene            6939..8372
                     /gene="mco"
                     /locus_tag="POH73_002174"
     CDS             6939..8372
                     /gene="mco"
                     /locus_tag="POH73_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-copper oxidase Mco"
                     /protein_id="HDI6888103.1"
                     /translation="MYKKMFTILITLFSIMFMVPNDTFAEGKHNMMDMKENDQKRNDM
                     MDMKSHDERKNLNSSQGKNEITFPKVLDPKKDNNGYKSYTLKAQKGKTEFYKGNFSNT
                     LGYNGNLLGPTLKLKKGDKVKIKLVNNLDENTTFHWHGLEIDGKVDGGPSQVIKPGKE
                     KTIKFEVKQEAATLWYHPHPSPNTAKQVYNGLSGLLYIEDDKKNNYPSNYGKNDLPII
                     IQDKTFVSKKLNYTKTKDEDGTQGDTVLVNGKVDPKLTTKEGKIRLRLLNGSNARDLN
                     LKLSNNQSFEYIASEGGHLEKTKKLKEINLAPSARKEIVIDLSKMKEEKVNLVDNDET
                     VILPIINKEKSTNKDTTPKVDKKIKLEGMDDNVTINGKKFDPNRIDFTQKVNRKETWE
                     IENVKDKMSGMKHPFHIHGTQFKVLSVDGKKPSEDMRGKKDVISLEPGQKAKIEVVFK
                     NTGTYMFHCHILEHEDNGMMGQIKVTK"
BASE COUNT         2904 a         1219 c         1398 g         2901 t
ORIGIN      
        1 ctacagcttt ttatgacctc acacaaaaac attttcgaag ccttgaaata tctggctttg
       61 ccagatcccc cattcattga tggggacaaa tttcccttat gctcttacgg agtttttaga
      121 gaaaaattga aatttatcga tttttgataa aaaacgccct gcaggaattt agaaaaacat
      181 gggaaagttt taaagaattt tatgctcatt tttaatttgc atgtaaatgg gcagtgttaa
      241 aaaattagtc actggttttg ctgaaatttt tgtttgtgaa attaccatat atttatttgg
      301 ctcgtatttt cgttttaaga gcttatataa gttttagata gaaaactacg ctatttatct
      361 tttaatcttt aaaatccccc tttaaaattc aaaatagggg cattcattat tttagtaagt
      421 cttgtgataa agtttgttaa aaaattggag gagtctatta tgaaaaagtt ttttatgttt
      481 tttgtttgtt tattaactgt gtttttaagt tttaattttg ttaattcgat tttatttgac
      541 ggttcttttg tctttttaga aagttttatt tattcattgg tttttacact cgttttttta
      601 ggattagagg gggataagga gaaaaagaag taggaaacgc atttatgccg agaaaattta
      661 ttgatattga gaagaaccct taaccaaaat tgaagacgaa tgtcggcata gcataagcta
      721 ttaagccgac tattcgacaa gtttagggat tgttaagggt tccgaggctc aacgtcaata
      781 aagcaattgg aataaagcaa tatagatttg ctatctaata atctttagaa gtttttttca
      841 ttaatttttc aaaatcagta tcgctaatat ttgattgagt aagtgtgatt aagtttccat
      901 ctggatcttt tattgtcatt tctttagcgt tccatggttg ttcttctaat tggcttacta
      961 taaatttttg tgaaatgttt tgtgattttt cttcgatatc ttttactaag atatttaagt
     1021 atattgagtt tccaacttca aaatcttttt gagaaattag cattaagtct tgatatttct
     1081 ctagtctcaa atgattcatt agcactttat taaacattgg catttgataa aattccattg
     1141 ttttcacctc tttttttatt tattgtaatg tattacgtta cgttaatgtc aaaaagaggt
     1201 ggtttttgaa acgagtttct tcttgtcttg atactataaa aaaacaaagt tatataacat
     1261 aagacgactt atcggaagta gatatgcttg tatattcaca gatggtataa taatagagtc
     1321 gcctatctct caggcgtcaa ttgacgaaga gaggaggtgc attactttgc taatcatttt
     1381 cgttcacatc atagcaccag tcatcagtgg ctgtgctgtt gcgtattata cttattggct
     1441 tagtaaacgc aacaaataat taataggcga ctttataagc cagcatcgaa aaaatcccca
     1501 cactactgca atagtgtggg gattggtgca ttaattgtta atcattttcg ttatatagat
     1561 tataacactg gttagtaaag aaatgcaaag tgatatgtac agtatcagtt acaaggtatc
     1621 gtttcaatat tttgacgcta attgtcaata atttaaagga taatttatga atgttatgta
     1681 ttgagagttt atcctttttt tatatagatt gtcattagaa cgtgcattaa atttagaata
     1741 aataatacaa agatcaaaat tcatgaaatc aaatttttaa taacttcatt aagcttcgta
     1801 tcaatatact tatccttatg tatgactaaa tagaaataac gtttgatgtc taagtttgtt
     1861 acttctagct tttctaaatc ataaggtgtt aatgttgatt tagaaacaat agaaaagcct
     1921 ttatcagcgt ggaccatatt tttgattaga tttgtgttgt taatttctac taaataagag
     1981 gatgaagtca tttgtgataa cgcattttcc tgatagactc ttgtccctga tccacgttct
     2041 ctgataaaac atattgactt atttgaagat gatgattttt tgcgaattaa tactatttca
     2101 tcttgagcaa tacgtctact gtgtataata ttactttgaa cctctttttc gattatacct
     2161 aaatcgatag tgttattctt aatatgttgt aatactgtat cagaattatt taaatacaca
     2221 tgaatgtata attcaggata atttttggct aaatcagcaa gatgttgtga caatcgatat
     2281 tcgccataag tgtaactact ccctatcgtt aaatctcctg aaattatatc aggttgcatt
     2341 ttcaaatcgt gtcttaagtt ttgttctaat cgctcacgtt gtaaagcata ttgaaatagt
     2401 ttctccccat cacttgttaa agacatttta gaatgtctaa attcgaaaat ttttacatcg
     2461 tattccagtt ctaatcgttt aatatcgcga cttattgatg gctgtgaagt atataaattt
     2521 tctgcagctt tagtaaaact ttgcgtttta actacttcag ttagaacttt taatgaatcc
     2581 attaactagg tctcctgaca tttgaatacc tctattataa caaaaatgtt atgagtaata
     2641 tacttaatat ttattttacg ttataactat gcgatgctaa gcttttgata aaggaggagt
     2701 taaaaagtga agtccttaaa aagcaaagcc tttatttatg gtttattatt cacatttatc
     2761 atcgcagtta taagtttatt aggctcaaag ttaccatttt tagataagat aggtgcttta
     2821 acaattgcta tactgattgc gattctatat agacatttta agggttaccc tgaagcatat
     2881 cgttcaggaa tcgcattttc gtctaaacgg ttactgaaat tagctattat attatatgga
     2941 ttaaagttaa atatttatga tgtaattgga aaaggtagcg atttggtatt aatcgatatt
     3001 ggagtcatcc tttttagtat cggattaatg ttgttactaa ataaatatat taaaggagat
     3061 aaaaacctca tcctattatt aggtattggc actggggtgt gtggcgcggc agctattgca
     3121 gcagtatctc caatattaaa atctagagaa aaagattctg cgattagtat tggtattgtc
     3181 gcacttattg gcactatatt ttcgcttgca tatactgtaa tatattctat ttttacaata
     3241 tctcctgaag tatttggggt ctggtcaggt acaagtttac atgaaattgc acatgtaatt
     3301 cttgcttcag acttctcagg ccaagaggct ttgagtatgg gactgttagg aaaactggga
     3361 cgtgtgtttt tattaatacc tctgagtatt attttaattt taattatgag aaaaaaatcg
     3421 catagtgaga atgaaaaaaa gcgtattgat gtaccttact ttttacttgg ttttgtcatg
     3481 atggcattat tccatactta tgtatccttg ccacacatgg ttatgcaaat tatagataat
     3541 atcactacta tatgcttatt gatggcaatg gttgcattag ggttaaatgt ttcatttaaa
     3601 gacttaaaag atcgtgcttt caaaccatta atagctgtca ttattgtatc tatttgttta
     3661 tcaactgtga catttattgt tgctaagtac ttttatagtt agactataag tatattaaaa
     3721 aattgatata gattctcaca aacaaacaca acacattgaa ggcattccat attactacgg
     3781 tcaaaaaaag atcagccttt ttcttactag gcctattctt ctttattatt actgacatat
     3841 caagctttac aatgcatatt ccgacttacc ttgacagttt aattatagta aatttcaata
     3901 tttctattaa ctatggaaca atataattta gggtacattc taatcataca aatgaagttg
     3961 ttgaatttat aatagcttaa acgtatgatt agtgtgtatt ttttaacttt tattattgtt
     4021 gaaagttaag ttgtatttac ttatcactct tttcatcttc taatttattg agataacgat
     4081 aaattgttgt acgtgaaact tgccattgtt cagcaattgt cttaattggc gtaccattat
     4141 cataaagtgt tttgagcaat tttaaatctt gtttgtttaa tttttcgggt cttccaccat
     4201 accgtcctct agcacgtgct gcaattctac cagctgaaga acgttctaaa attaaattac
     4261 gttcaaattc tgcaaatgct gcgaataaat gaaataataa ttgtccggta gaacttgatt
     4321 tatccatcgt tatattttct tctaaactat ggaaacttac acctcgttca ttaagctcat
     4381 taactaatgt aattaaatcc tccatattac gtcctagtct atctaatctc caaacaacaa
     4441 tagtatctcc agaccttgca aattcaattg ctttatctaa accaggtctt ttactttttg
     4501 aaccactcat atgatcgtta aatatctttt cacaaccata tgcatttaat cgatcttctt
     4561 gtaaatttaa attttgtaat cctgttgaaa ctcttgcata acctattttc aaaatacaaa
     4621 cctcctatat ccatcattag tgtaccataa cttaaatacg ggtactttta tgaacataga
     4681 ttttttattg ggtttctgaa cataaaaata aatgtaatcc tcacttattt cgatataaaa
     4741 aaagagtgta cagaaaatgg ttattttctg tacactacaa aataaaaaag tcaaatggta
     4801 tataccccta tcccgtatat gttacactca aattgtctta ttactatctt taaaaatata
     4861 aaattaggag gtttaatttt gtcgaataac aaaaaacacc aaaatcatga gcatcatagt
     4921 catcaagaac atgaaaatca cactagtcat ggtaatcatg aacatcacca tcatggaaat
     4981 tttaaaagta aattctttat ttctttgatt tttgcgatac cgattattat tttatcgccc
     5041 atgatgggag ttaaattacc gtttcaaatt tcatttacag ggtcagattg gattgtatta
     5101 attcttgcta caattttatt cttttacgga ggaaagccat ttttatcagg ggctaaagat
     5161 gaaatttcaa ctaaaaaacc tggaatgatg acgcttgttg ctctaggtat ttcagtagct
     5221 tatatttata gtttatacgc cttttatatg aataacttca gtggttcatc cacacataca
     5281 atggactttt tctgggaatt agcgacttta attttaatca tgttattagg tcattggatt
     5341 gaaatgaacg cagtaggtaa tgcaggcaat gcactgaaga aaatggcaga acttttacct
     5401 aatactgcag tgaagctgat tgataacaat caacgagaag aagttaaaat atcagatatt
     5461 catattgacg atattgtgga agtcagagca ggcgaaagta tacccacaga cggaatcatt
     5521 gttcgaggag aaacatcaat tgacgaatca ttagttactg gcgaatcaaa aaaagttcat
     5581 aaaactcata atgatgatgt tattggaggt tcaattaatg gttcaggtac agtacaagtt
     5641 aaagtgactg ctacgggtga aaatggctac ctatctcaag ttatgggtct tgttaaccaa
     5701 gctcaaaacg ataagtcaaa agctgaactc ctctctgata aagtcgcagg ttatttgttt
     5761 tactttgcag taagtattgg cctgatttca tttatcgtat ggatgcttat tcaaaacaac
     5821 gtcgatttcg cacttgaacg gctagtaacc gtattagtca ttgcttgtcc acatgcatta
     5881 ggcttggcaa tacctttagt tacagcacgt tctacctcta taggcgcaca taatggttta
     5941 atcattaaaa acagagaatc tgtagaaatc gcacaacaca ttgattacat catgatggat
     6001 aaaactggga cactaactga aggtaacttt tcagtgaatc actatgaaag ttttacagac
     6061 gagttaaata atgaagaaat attgagtcta tttgcttcat tagaaagtaa ttctaatcat
     6121 ccacttgcca ctggtattgt tgattttgct aaaggcaaaa atatttcata tgctactcct
     6181 caagaagtta ataatattcc aggtgtaggc ttagaaggca cagttgataa taaaaaactc
     6241 aaaatagtga atgtttctta tctcgataaa agcaattttg attataataa agaacaattt
     6301 acgaatctcg ctcaacaagg gaattctatt agttatctaa ttcatgaccg acaagttatt
     6361 ggtattatcg cacaaggcga taaaattaaa gaaagttcaa aacaaatggt ttcggattta
     6421 ttatcgagaa acattacgcc agtcatgctt acaggcgata ataaagaagt ggcacaaaca
     6481 gtggctgaag aattaggtat tagtgatgtc cacgcgcaat tgatgcccga agataaagaa
     6541 agtattatcc aagattatca aagcaatggt agcaaaatca tgatggttgg tgatggtatc
     6601 aatgatgcgc caagccttat tagagcagat attggtatgg caattggcgc agggacagat
     6661 gtagctattg aatcggggga tgtcatactc gttaaaagta atccttctga tattatcaat
     6721 tttttgtccc tttcaaaaaa tacgatgaaa aaaatggttc aaaatttatg gtggggcgca
     6781 ggatataacg tgattgcagt tcctcttgca gctggtatat tagcttctat tggtttaata
     6841 ttatcacctg ctgtgggtgc aatacttatg tctttaagta cgatcatcgt agcaattaat
     6901 gcatttacat taaaattaaa ataaaagagg taaatataat gtataaaaaa atgttcacaa
     6961 ttttaattac gttattttct ataatgttta tggttcctaa tgatactttt gcagaaggta
     7021 agcacaacat gatggacatg aaagaaaatg atcaaaagcg aaatgatatg atggatatga
     7081 aaagtcatga cgaaagaaaa aatttgaatt cttcacaagg aaaaaatgaa ataacatttc
     7141 ccaaagtttt agatcctaaa aaagataaca atggttataa aagttataca ttaaaagcgc
     7201 agaaaggaaa gacagaattt tacaaaggta atttctctaa cactttagga tacaatggaa
     7261 atttacttgg gccaacttta aaattaaaaa aaggagataa ggttaaaatt aagttagtca
     7321 acaacttaga tgaaaataca acatttcatt ggcatgggtt agaaatagat ggaaaagtgg
     7381 atggaggccc ttctcaagtt ataaaaccag gaaaagaaaa aacaataaaa tttgaggtta
     7441 aacaagaggc tgctacatta tggtatcatc cccacccctc cccaaataca gctaaacaag
     7501 tttataatgg tttatcaggg ttattatata tagaggatga caaaaagaat aattatccta
     7561 gcaattatgg gaaaaatgat ttgcctataa taatccaaga taaaacattt gtatctaaaa
     7621 agttaaatta tacaaaaacg aaagatgaag atggtactca aggtgataca gtccttgtga
     7681 atgggaaagt agatcctaaa ttaacaacaa aagaaggaaa aatacgtttg agacttttaa
     7741 atggctccaa tgctcgggat ttaaatctta agctaagtaa taatcaaagt tttgaatata
     7801 ttgcttcaga aggcggccat ttagaaaaga ctaaaaagtt aaaagaaatt aatttagctc
     7861 cttcagcaag aaaagaaata gtaatagatt tatctaaaat gaaagaagaa aaagtcaatc
     7921 ttgttgataa tgatgaaaca gtaattttac caattattaa caaagaaaaa agtactaaca
     7981 aagacactac tccaaaagta gataaaaaaa tcaaattaga aggtatggac gataatgtta
     8041 ctattaatgg caaaaaattc gatcctaaca gaatagattt cacacaaaag gtaaaccgta
     8101 aagaaacttg ggaaattgaa aacgtcaaag ataaaatgag tggtatgaag cacccgttcc
     8161 atatccacgg aacgcaattt aaagttttat cagtggatgg gaagaaacct tcagaagata
     8221 tgagaggcaa aaaagacgtt atatctctgg aacctggaca aaaagccaaa atagaagtgg
     8281 tttttaaaaa tactgggaca tacatgttcc attgtcacat acttgagcat gaagataatg
     8341 gaatgatggg acaaataaaa gtaacaaaat aatttatatc gtcgcgatca ttttatcatc
     8401 tccaaatatt ttagggatga gg
//
LOCUS       DAMZPP010000121         8303 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMZPP010000121 DAMZPP010000000
VERSION     DAMZPP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.121"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..176)
                     /locus_tag="POH73_002175"
     CDS             complement(<1..176)
                     /locus_tag="POH73_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDI6888104.1"
                     /translation="MGYLKRIGMCISLLIVIIFVTSCGGGNKITGDSKETQIKKSFAK
                     TLDMYPIKNLEDLY"
     gene            complement(214..1016)
                     /locus_tag="POH73_002176"
                     /pseudo
     CDS             complement(214..1016)
                     /locus_tag="POH73_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            complement(1047..1820)
                     /gene="lpl1"
                     /locus_tag="POH73_002177"
     CDS             complement(1047..1820)
                     /gene="lpl1"
                     /locus_tag="POH73_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HDI6888105.1"
                     /translation="MGYLKRFALYISVMILMFAIAGCGKGNETKEGSKETQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGHYFVTTFYRNGKLPDEKNYKIEMKNNKIILLDEVKDDKLKQKIENFKFFGQ
                     YANLKELKKYNNGDVSINENVPSYDVEFKMSNKDENVKQLRSRYNISTEKSPILKMHI
                     DGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLSYKPAKK"
     gene            complement(1868..2649)
                     /locus_tag="POH73_002178"
                     /pseudo
     CDS             complement(1868..2649)
                     /locus_tag="POH73_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            3016..3324
                     /gene="spn"
                     /locus_tag="POH73_002179"
     CDS             3016..3324
                     /gene="spn"
                     /locus_tag="POH73_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HDI6888106.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNEADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
     gene            complement(3430..4917)
                     /locus_tag="POH73_002180"
     CDS             complement(3430..4917)
                     /locus_tag="POH73_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HDI6888107.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASEANEKPSTNQE
                     SKVVSQTEQNSKETKTVESNKNFVKLDTIKPGAQKITGTTLPNHYVLLTVDGKSADSV
                     ENGGLGFVEANNKGEFEYPLNNRKIVHNQEIEVSSSSPDLGEDEEDEEVEEASTDKAG
                     VEEESTEAKVTYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKGHTSVKGK
                     VALSINNKFINFEESVKGGVSKEDTKASSDGIWMPIDDKGYFNFDFKTKRFDNLELKE
                     GNDISLTFAPDDEEDALKPLIFKTKVTSLEDIDKAETKYDHTKLNKVKVLDNVKEDLH
                     VDEIYGSLYHTDKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEAQLFPDLQVNEKG
                     EFSFDSHGAGFRLQNGEKLNFTVVDPITGDLLSNEFVSKEIDIEETPEQKADREFDEK
                     LENTPAYYKLYGDKIVGFDTNDFPITWFYPLGEKKVERTTPKLEK"
     gene            complement(4936..5634)
                     /locus_tag="POH73_002181"
     CDS             complement(4936..5634)
                     /locus_tag="POH73_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HDI6888108.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASEQSRLSVTSNDTQ
                     ELKKYYSGTGYNFQNVSGYREKDKMNIIDGTQLNVVTLLGTDKERFKDYDYDYEGLDV
                     FVVREGSGKQAENISIGGITKTNKNDYKDFVNNVGLEITKPTGHNTATRQAETYRINK
                     EEISLKELDFKLRKHLIENHELYKTEPKDGKIRITMKGGGYYTFELNKKLQPHRMGDV
                     IDGRNIEKIEVDLY"
     gene            complement(6017..7249)
                     /locus_tag="POH73_002182"
     CDS             complement(6017..7249)
                     /locus_tag="POH73_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI6888109.1"
                     /translation="MSNTQTKNVPELRFPGFEGEWEEKKVGELLEFKNGLNKGKEYFG
                     SGSSIVNFKDVFNNRSLNTNNLTGKVNVNSKELKNYSVEKGDVFFTRTSEVIGEIGYP
                     SVILNDPENTVFSGFVLRGRPKSGIDLINNNFKRYVFFTNSFRKEMITKSSMTTRALT
                     SGSAINKMKVIYPVSAKEQRKIGDFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQE
                     LRFKDENSEDYPHWENSKIEKYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSK
                     DKSNYKVVRKNDIAYNSMRMWQGASGRSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKT
                     HRMIHKFKINSQGLTSDTWNLKYKQLKNINIDIPVLEEQEKIGDFFKKMDILISKQKI
                     KIEILEKEKQSFLQKMFL"
     gene            complement(7242..>8303)
                     /locus_tag="POH73_002183"
     CDS             complement(7242..>8303)
                     /locus_tag="POH73_002183"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDI6888110.1"
                     /translation="LDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQV
                     SKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKEAKVYRYFGQERNNTTYNLARMNM
                     LLHDVRYENFDIRNDDTLENPAFLGHTFDAVIANPPYSAKWTADSKFENDERFSGYGK
                     LAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGIIRRYLIEEKNYLEAVI
                     GLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDT
                     YKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIA
                     EIEQEINAYLKELGVLKDE"
BASE COUNT         2380 a         1551 c         1007 g         3365 t
ORIGIN      
        1 tcatataggt cctcgagatt tttgattggg tacatatcta acgttttcgc aaaactcttt
       61 ttgatttgtg tttcttttga atctccagtg atcttattac caccaccgca agatgttaca
      121 aaaataatta caatcaatag gcttatgcac attccaatcc tttttaaata tcccatcttt
      181 tcacatctct ctgttttaga attatttcta cacctaataa actttagctc ctccatcaga
      241 tggtccaaaa ttcacaaaat ctcttacagc tgtttcttgg tcttttactt ttgaaaaatt
      301 atattctata tttttataac caactgaact tccttttatg tcgccatcta tatgtaattt
      361 taatactggc gactttttgg ttgtaatagg ataaacttct ctaagttttt ttacattttt
      421 atccgtatta ctcattttat attctgcatc gtagcttggc acattatctg tacttgatat
      481 atttccattt ttataatatt taagatctct aaaatcagca tactgactaa agaatttaaa
      541 attttctatt ttatttttga gatttggatc ttctacttta tccaaaagta ctatcttatt
      601 attttttaat tcaacatgat atttttttgt tgatcttttt cattaaattc atcatgaact
      661 ttgctcgtat aataatatcc tgttgccttc tttgtatttc tattaaggaa taaaaccata
      721 ccttcgtcat ctatctcgcc tggtttgtta cttttagcaa aacttgtgag taaagtccat
      781 gtacccttat cgcctttttt aaattcgtca tctcgataac cttctttgtc gtataaatcc
      841 tcgagatttt tgattggata catatctaac gttttcgcaa agctcttttt aatttgttct
      901 tcttttgaat cttcttttgc tttttccgca gtatcgcttg aactattaca acttattata
      961 aaaatgctca aaataatagc acttatgaac agtgcaagct tttttagatg tcccattttt
     1021 tcacacccta atattcctta tcaatactat tttttcgctg gtttataact taaaaattca
     1081 atgactgata atttgctgtt ttcacgtttt gaaaagtcaa tttctaactt tctataacct
     1141 acggaactgc ctttcaggtc accatcaata tgcattttta atataggtga tttttcagtc
     1201 gaaatattat aacgacttct taattgctta acattttcat ctttattgct cattttgaat
     1261 tcaacatcat aactaggaac attttcatta attgaaacat caccattatt atattttttc
     1321 aattctttaa gatttgcata ttgaccgaaa aatttaaaat tttctatttt ctgcttgagc
     1381 ttatcatctt ttacttcatc taataaaata atcttattat ttttcatttc aattttataa
     1441 ttcttttcat ctggcagttt accattccgg taaaatgtag taacaaaata atggccttta
     1501 gcggttctag tattcctatc cagatttaaa accatacctt catcatccaa ttcacccggt
     1561 ttattacttt tagcgaaatc tgtgtatatc gtccacatcc ctttgtcacc tttttcaaat
     1621 tcaccatctc tatagccttc tttgtcgtat aaatcctcga gatttttgat tggatacata
     1681 tctaacgttt tcgcaaagct ctttttgatt tgtgtttctt ttgaaccttc ttttgtttca
     1741 ttacctttgc cacaacctgc tatcgcaaac attaaaatca taacgcttat gtacaatgca
     1801 aaccttttta aataccccat cgtttcacgt ctctctgttt tagaattatt tctacatcta
     1861 ataaacttta gctatcttca tcagacggcc caaaatttaa ataatctctt aatgttgtct
     1921 cttgatcttt aacttttgaa aatttatatt ctattttttt gtatccaact gaacttcctt
     1981 ttatatcacc atctatatgt aactttaata ttggagcctt ttcgttgtaa ttggataaat
     2041 atctctaagt ttttttacat tttcatcact attattcaat ttgtattctg cttcataact
     2101 aggaatattt tcattagttg ttatacttcc atcttgataa ttttttaaat ctttaaaatc
     2161 cgcatactga ctaaaaaatt taaaattttc aattttttgt ttaagttttt cgtcttctac
     2221 attatccaaa agaacaatct tattattttt aagttcaaca cggtattttt tctcattctg
     2281 atttttgcta atatcatcat aaattttatt tacaaaataa taacctgttg cctttttggt
     2341 atttctattt agatataaaa ccatgccttc gtcatctatt tcatccggtt tgttactttt
     2401 tgaaaaactt gtgagtaaag tccacgtccc cttatctccc tttttaaatt cgccatctcg
     2461 atatccttct ttatcatata ggtcctcgag attcttaatt ggatacatat ctaatgtttt
     2521 cgcaaagctc tttttaattt gttcttcttt ggaatcttct tttgttttat cacttttgcc
     2581 acaacctgct acaacaacta ttaaaagcaa gatacttatg tacaatgcaa aactttttat
     2641 atttcccatc atttcacgtc tctctgtttt atacttcatt tatattctct tttcatataa
     2701 ataattaatt attttgtata tttctctttt acgttaccaa aatatacata tacaatcaat
     2761 ttaatttatt aattaaattt gtacttatta ttatgttgca tcattttttt attattgttt
     2821 catttgaatt aaaatatctt cgaaatctac tactcaatac taaggaacac aagtatcgca
     2881 ctaaagatta attgcattta aacaatattt gtcactttaa ctatttatta aaaatcatta
     2941 aatcgactta ataaacgtaa ttttctcaat attcagtgtt atgatggagt tacaattttt
     3001 agaggagtga tagatatgaa atttaaaaag gttttagtag ctacggcaat ggtaggtgta
     3061 ttagcaactg gcgttgttgg atatggtaat gaagcagatg cgaaagtgta ttctcaaaat
     3121 ggactcgtac tacatgatga tgcaaacttc ttagaacatg aattaagcta tatcgatgtg
     3181 ttattagata agaatgctga ccaagctaca aaagacaact taagatcata cttcgccgat
     3241 aaaggactac attcaatcaa agacattatc aacaaagcta agcaagacgg ctttgatgtt
     3301 tcaaaatatg aacacgtaaa ataaattcaa taatgcttta taacaaagca cataaaaaaa
     3361 cagctcaatg acgtagaagt cagtgattaa ttcactaatt tcattacatc attaagctgt
     3421 tttatttaat tatttttcta atttaggtgt tgtacgttca actttctttt cacccaatgg
     3481 atagaaccaa gtaatcggga aatcgttagt atcgaatcca actattttat cgccgtataa
     3541 cttgtagtaa gcaggcgtat tttcaagttt ttcgtcaaac tcacgatccg ctttttgttc
     3601 aggtgtttct tcaatatcaa tctctttaga aacaaactca ttacttaaca agtcacctgt
     3661 aataggatca accactgtga agtttaattt ttctccattt tgtaatctaa aaccagcacc
     3721 atgtgagtca aagctaaatt cacctttttc atttacttgt aaatcaggga ataattgtgc
     3781 ttcacctagt tcagagtcta ccttcactac tgcattcgcg aatttagtct ttcctttaat
     3841 tactttagta ccttctttat caagaatacc tttacctttg tctgtatgat ataagcttcc
     3901 atatatttca tcaacatgta aatcttcttt aacattatct aaaactttca ctttgttgag
     3961 tttagtatgg tcatatttag tttctgcttt atcgatatct tctaagctcg ttactttagt
     4021 tttgaaaatt aaaggtttta atgcatcttc ttcatcatca ggtgcaaatg ttagtgaaat
     4081 gtcattacct tcttttaact ctaaattatc gaaacgtttc gttttgaagt caaagttaaa
     4141 gtatcctttg tcatcaatag gcatccagat accatctgaa ctagctttag tgtcttcttt
     4201 actaactccg cccttaacgc tctcttcaaa attaataaat ttattattaa tagataaagc
     4261 aactttacct tttacagacg tatgcccttt aataatacct gatccttcag taataggttc
     4321 gataaaaact tggtgatgtc catctttttc ttttagttgt tctttcggta tttcatacgc
     4381 tttttcatat cgcggtgttg tgtaagtaac tttagcttct gtactttctt cctcaacgcc
     4441 agctttatca gttgaagctt cttccacctc ttcatcttct tcatcttcac ctaaatcagg
     4501 gcttgacgac gaaacctcaa tttcttgatt atgaacaatt ttacgattat ttaaagggta
     4561 ctcaaattct cctttgttat ttgcttcaac aaaacccaaa ccgccatttt ctactgaatc
     4621 cgcacttttc ccatcaactg ttaataaaac atagtgattt ggtaaagtag ttcccgttat
     4681 cttttgagct ccaggtttaa tagtatctaa tttaacaaag ttcttattag attctactgt
     4741 ttttgtttct tttgaatttt gttcagtctg tgaaacaact tttgattctt gatttgtact
     4801 tggcttctca ttagcctctg atgcttctgc ttgtccgttt gtcatgatat ataacattgt
     4861 aattgcaaca gatactaacc cgactttcat tttacgtaac ttaaaatttt ccctcatgat
     4921 atactccctc gaatattaat ataaatcgac ttcaattttt tctatatttc taccatcaat
     4981 tacatcaccc atacgatgag gctgtaattt tttatttaat tcaaaagtat agtagccgcc
     5041 acctttcata gtaattctaa ttttaccgtc tttaggctct gtcttataaa gttcatgatt
     5101 ttcaattaaa tgttttctta atttgaaatc taattctttt aatgaaattt cttctttatt
     5161 aattctataa gtttctgctt gtcttgttgc tgtattatgt cctgttggtt tagttatttc
     5221 tagacctaca ttatttacga aatctttata atcgttttta ttcgtcttgg taattccacc
     5281 tattgaaata ttttcagctt gtttacctga tccttctctg actacaaaga catctaaccc
     5341 ttcataatca tagtcataat ctttaaatct ttctttgtct gtaccaagta aagtaactac
     5401 attaagttgt gtcccatcaa taatgttcat tttatccttt tctctataac cactcacatt
     5461 ttgaaaatta tatcctgttc cactgtagta tttttttaat tcttgcgtgt cgtttgaagt
     5521 aactgataat ctgctttgct cacttgcttt tactggctga gcagtagttg taatcatccc
     5581 tgttgtcaaa atccctagtg ctaaacttgc tttagcaata ttttttaatt tcataattct
     5641 atgctcccaa tttttagtct atttgattta ttctattacg caatacgaac aatcctcatt
     5701 cattataatg agcaacggtc gtgcttcaca ttaaacttac tttaactaaa aattaatcat
     5761 tattaagtga tttaataaat agttaactaa tatcattcaa ttcctatcga tctactctct
     5821 tttatttacg aataacactt tatctcaact taatctttat ttaactactt attctgtaca
     5881 caatttcgac acaaaaaaga cactgtgcta tcacagtgcc taaatttaat gttcgttttt
     5941 aatttacttt atgtttataa cttttatgtt aaattacaca aattttattc ttatgcaacc
     6001 tatgtgttta tcaaagttat aagaacatct tttgtaaaaa ggattgtttc tctttttcta
     6061 atatttcaat ttttattttc tgtttactaa tcaatatatc cattttttta aagaaatcac
     6121 ctatcttttc ttgttcctcc aatacaggta tatctatatt tatatttttt aattgtttat
     6181 attttaagtt ccatgtatct gatgttaatc cttgtgaatt aattttaaat ttatgaatca
     6241 ttctatgtgt tttaaactta tatccaataa ataatgagct agtattttgt gttggataaa
     6301 gcacagtata tgcagggcta acaatcccat tataatttga tctaccacta gccccttgcc
     6361 acattctcat agaattatat gcaatatcat ttttcctaac tactttataa ttacttttat
     6421 ctttacttga attatctttt ctatccaatt cactaaattt tataatgcca ctatttatag
     6481 ttactgaaag catttgacct ttgtcagaac gttcgtttct ctcttttaaa tatttttcta
     6541 ttttgctatt ttcccaatgt ggataatctt cactattctc atctttgaat cgcagttcct
     6601 gtgagaagat tttctgcata tagccttttt tttgttgttg aagtaattca agcttttgtt
     6661 cttctaattc aatttgtcgg tcgagtttgc tgaagaagtc acctattttt ctctgttctt
     6721 tagccgaaac agggtatatg accttcattt tattaattgc gctacctgat gttaaagctc
     6781 tagttgtcat agaacttttt gtaatcattt cttttctaaa tgaattagta aaaaagacat
     6841 atcttttaaa attattattt attaaatcaa ttcctgattt aggccgccct cttaatacaa
     6901 atccactaaa cacagtattt tcagggtcat ttaaaattac agacggataa cctatttcac
     6961 caattacctc actagtcctt gtaaaaaaaa catcaccctt ttcaacagaa taatttttta
     7021 gttctttgct attcacatta acttttccag tcagattatt tgtatttaag ctcctgttat
     7081 taaatacatc tttgaagtta acaatcgacg atcctgagcc aaaatattct tttcctttat
     7141 ttaaaccatt tttaaattct aataactcgc caaccttctt ctcttcccat tcgccttcaa
     7201 accctgggaa tctcaactct ggcacatttt tcgtttgtgt attactcatc tttcaacacc
     7261 ccaagttctt tcaggtatgc attgatttct tgttcaattt ctgcgatttc tttgtcgata
     7321 tttttcaaat cttgttggac ttgatctaaa tcaattggcg cttcttcttc gaatgtatcg
     7381 acatatctcg gtatgtttaa gttgtaatcg ttatcggcga tctcttgtaa tgtcgcgctg
     7441 tagctatact tatcaattgt ttccttacgc ttatatgtgt ctataattcg ttcgacttgg
     7501 gcatcgctta aatggttttg gttttttcct ttttcaaaat cattggatgc atcgataaat
     7561 aatacgttgt cgtcttgttg gcgacatttt ttaaatacta agatacatgt tggaatactt
     7621 gtcccataga aaatgttcgc tggcaaacca atcacggctt ctaagtagtt cttttcttca
     7681 attaaataac gacgaatgat accttctgcg gcaccacgga ataagacacc atgtgggagt
     7741 acaacggcca tggtaccttc atcatctagg taatgtacca tgtgttgaat aaaggcaaag
     7801 tctgctttag actttggcgc aagcttgccg tatccgctga agcgttcgtc attttcaaat
     7861 tttgagtctg ctgtccattt cgcgctatat ggtgggttcg caataaccgc atcaaatgta
     7921 tgtcctaaaa aggctggatt ttccaacgta tcatcattac ggatatcgaa attttcatat
     7981 cgcacatcat gtaacaacat gttcatgcgc gctaagttgt atgtggtatt gttacgttct
     8041 tgtccaaaat aacgatacac ttttgcctct ttaccaacgc gtaacagtaa tgaacctgaa
     8101 ccacatgttg ggtcatatac atgacgtaat ttatctttac cgtctgtgac aatcttcgcc
     8161 agtatcttag atacttgttg tggtgtatag aactcgcctg cttttttacc cgctgtcgcc
     8221 gcaaagcgcc cgataagaaa ttcgtatgca tcacctaaca tatcaatttc catatcactg
     8281 tgaacgaatg gtaagtcatc aag
//
LOCUS       DAMZPP010000122         8265 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMZPP010000122 DAMZPP010000000
VERSION     DAMZPP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.122"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..570
                     /locus_tag="POH73_002184"
     CDS             <1..570
                     /locus_tag="POH73_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDI6888111.1"
                     /translation="ENVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTVIYGFVATCIV
                     LPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVIILLLLAQIFY
                     FYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPVIWKGLLFIEVLLAT
                     VIVLRVFIGYLHFFLKKENQDKFLRNASQ"
     gene            complement(886..1113)
                     /locus_tag="POH73_002185"
     CDS             complement(886..1113)
                     /locus_tag="POH73_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888112.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVTYANMVFDWNLRWYLLGNI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
     gene            complement(1420..2976)
                     /locus_tag="POH73_002186"
     CDS             complement(1420..2976)
                     /locus_tag="POH73_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDI6888113.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKKDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
     gene            complement(3147..4079)
                     /gene="arcC"
                     /locus_tag="POH73_002187"
     CDS             complement(3147..4079)
                     /gene="arcC"
                     /locus_tag="POH73_002187"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDI6888114.1"
                     /translation="MAKIVVALGGNALGKSPQEQLKLVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFTFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            complement(4102..5103)
                     /gene="argF"
                     /locus_tag="POH73_002188"
     CDS             complement(4102..5103)
                     /gene="argF"
                     /locus_tag="POH73_002188"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDI6888115.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            5533..6258
                     /locus_tag="POH73_002189"
     CDS             5533..6258
                     /locus_tag="POH73_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HDI6888116.1"
                     /translation="MKKNITNKIVLSTALLLLGTTSTQPPKAPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHLSFSGKWLWQNPNGTIHATLQTWVWYTHIQVFGPESWGNINQL
                     RDKYVDIFGTKDEDTIEGYWTYDETFTGGVTPAATSSDKPYKLFLKYKDKQQTMIGGL
                     EFYQGNKPVITLKELDFRVRQTLIKNKKLYNGEFNKGQIRITGGGNHYTIDLSKKLKL
                     TDTNRYVKNPRHAEIEVILEKSH"
     gene            6368..7093
                     /locus_tag="POH73_002190"
     CDS             6368..7093
                     /locus_tag="POH73_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HDI6888117.1"
                     /translation="MKNNLTKKIILSIALTMIGTATSQSPHSPISMSSEAKAYNISES
                     ETNVNELTKYYTQRHLTFSNKWLWQKDNGTIHATLLQLSWFSHIQVFGPESWGNINQL
                     RNKYVDIFALKDYETWRTYMLAQETFTGGVTPVAKPSDKHYKLNVTYKDKAGTFIGEY
                     QFYTGNKPVLTLKEVDFRIRQTLIKNKKLYNGDYNKGHIKITGGSNNYTIDLSKRLKS
                     TDANRYVKNPQTAHIEILLEKSS"
     gene            7202..7918
                     /locus_tag="POH73_002191"
     CDS             7202..7918
                     /locus_tag="POH73_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HDI6888118.1"
                     /translation="MRKCIMKKLILMTTLLLLGTTATQTSNSPLNVSTDAKAYHIGQD
                     ETNINELIKYYTQLPLTFSNRWLYQYDDGNIYVEFKRYSWSAHIRLWGAESWGNVNQL
                     RDRYVDVFGLKDEDTSQLWWVYRDTFTGGVTPAASPSDKPYSLFVQYKDKLQTIIGAH
                     KMYQGNKPILTLKEIDFRARETLIKNKILYHENRNKGKLKITGGGNDFTIDLSKRLHS
                     DLANVYVKNPQKITVEVLID"
BASE COUNT         2840 a         1493 c         1184 g         2748 t
ORIGIN      
        1 gagaatgttt atccttcatg gactgtactt tttattggta ttgctatcgc aggattgacg
       61 gcacccgtca gcggatattt tttcataggt caattaacag taatatatgg ctttgtagct
      121 acttgtattg tcttacctat agttttcaag cgattaaaag catttccatt gcagacgtca
      181 atcaaaccga acacatcgac aatttgtgca ccattttctt tagtcgctgc agcatatgtt
      241 atagcctttc ctaaagcgaa tgcttttatc gtaattatac ttttactatt agctcaaata
      301 ttttattttt acatcattat acaattgcct aaattactaa aagaaccttt ttcgccagta
      361 ttttcagctt tcacattccc tttagtaatc tctgcaactg ctttaaagaa cagtttgcct
      421 gtgcttatgt ttccagtcat ttggaagggc cttttgttta tcgaagtgtt attagccact
      481 gtaatagtac ttagagtctt tataggatat cttcacttct ttttaaaaaa ggaaaaccaa
      541 gataaatttc ttcgtaatgc gtctcagtaa cactattacc aagaatttac acgcatattt
      601 aataactaaa aaaggggcag gtaatcttgc ctctttttag tgtatatcaa tgcctctttg
      661 atttaatatc atcttattta tgaacaacaa tgataccttc tgactaaagc tgtagtaact
      721 atactagaac gtctagttca taaacacgtt tcactctatt ctttatttaa ataatatata
      781 agcagcaaac gccaaggcta atataccaat aaaataatta ataaagattt ttattttagt
      841 ttttgtaata gttgacaagt tttatcacct cttaacatcg ataaattaaa cgccgttatt
      901 tttcctctgc ctatctttta tcatttcaga aggtactccg aaaataaaca tcagaataaa
      961 taatataatt ggcaaatgtg gtatgtttcc tagcaaatac catcttaaat tccaatcaaa
     1021 taccatattt gcatacgtca caaaatattt actaaataat aatactacgc atattccagt
     1081 tagtgtatct aaaacctttg tcaaatgtgt catttatagc catcctttat ttatgctatt
     1141 ttcacaacca aatgtatcat gtgttacaaa aactgtttac agcaaatata tatttaccac
     1201 gataaatata atataccata gatctaacat tacattaaaa acatatttta aaaatatcat
     1261 tattaaatag aacattgtat caattactta ctaatgttat cattaccgcc attattttct
     1321 aaatcaaaat aataatgcac ctcgcttagt ttagtatttc tacaaaccta cgagatgcat
     1381 ataacccaat tagtattttt tataatatag catttcaaat tatgaataaa ttagtacttg
     1441 cgtaattagt actccgccac cgaaaatcaa tacaaaagca actaccggcc atacaaatcg
     1501 gaaccaatgt gaatatcgca tgtttaacat ttgtagtgtg gccataacaa gtccagtcgg
     1561 cgctaagaat aacatagcat attgaccaaa ttgatatgtc gtaacgatga cgaaccttgg
     1621 tatacctact gtatcagcta gtggtgcaaa gataggcatt gataataccg ctaatccaga
     1681 agatgatggc acgataaaac ctaaacagaa gaaaataaat agtaatacga tgataaataa
     1741 tggtccactc atatgttgaa ctaaagatga tgaaaagtgt aagattgtat ctgaaatcat
     1801 accttcattc aacactaaat taatacctcg agctaatcca ataattaaag atacacctac
     1861 taaacttgat gcaccattga caaatgcatc tacagtccct ttttctccca atccagattt
     1921 acctgtccca gcaataaaca ttattataat tgtaaatatt aaaaatgctg aagccataac
     1981 tgggaaccac caaccttgcg tcataactcc ccataccata attggaaatg gtagtacaaa
     2041 taatgtaagg attatcttct tacgcaaagt aaaatgggca ctatcgtcat cttttaacac
     2101 agaccattgc tgttcaaaag catctttgtc ttcataagaa tatgacgctt taggatcttt
     2161 tttaattttt ttacagtacc aatataaata actaataaca aaaatcgcac cgacaataca
     2221 agcacctatt ctccaataca aaccatccgt gaaagttgta ccagctgcat tagaggcaat
     2281 tacaaccgag aacgggttaa tagttgaaaa ggtactaccg acagagctgg caaggaatat
     2341 ggcaccaaca gaaacgatag aatcgtatcc taacgctata aatataggga ctaaaatcgg
     2401 atagaatgcg acagcttctt cttcaatacc acataacgtc ccgccgataa tcattagtat
     2461 tgatacaaac acaattagca taaattcatg ccctttagtt ttctttgtta aagctagcaa
     2521 tcccgattca aaagaaccac tggcttgaac tacgccaata agtcccccta atacaagaat
     2581 gaataccatg atatcaaccg cttctatcgt accttcaacc atgctacttg ttatttgttc
     2641 tggtccagct ggatgttgct ttaatctttc ataagtattc ggaattgata ccggcttatt
     2701 aattgcacct gatttaaatt gttcaatctt aattttaacc cccattttgt ctagttcctg
     2761 ttgcgtacct ggaacctttt tcacttggtt atgagggtta actatcttta gttcttggga
     2821 tgaaggttcg taagaaagtt tagaatatgc accagcagga ataacccatg ttgctataac
     2881 tgcaacaacc gttaaaatga ataaaatcat aaatgcacct ggcattttta atttaaatcg
     2941 ttttttcttt tcactttcat taattgtatt ttccacggtt tcatctcctt cgacatttaa
     3001 cctagcattt ctaccttaaa gattttataa atataaatta agaaagtgca ccccgcatca
     3061 aaatagaggc attattttca ggggtgcaca taaataataa aaatcatgca tttgacatat
     3121 agtaattgaa aagcgtttca attcaattac tttttaatca cagtacctac tttaccctct
     3181 aaggcagcat ctaattcatt taatgatgtt ataagcacac ttccttttgg attgttttca
     3241 ataaatgata tggctgcttc aatttttggt aacatacttc cttttgcaaa ttgattttcg
     3301 tctatatatc gttttaattc atcaacattt gttgttttca aaggctgttg gttttcagtg
     3361 ttaaaattaa tatatacata atcaattgca gttaaaataa tcaattgatc gcattgaata
     3421 ttagcaccca acaacgcact tgttttatct ttgtctataa ctgcatcaat acctttaaaa
     3481 ccatcatgtt gctctctaat tactggtata cctccaccac cagcagcaat aacgagtgta
     3541 tcatttttaa taagtgtttt aatactctct aattcaataa tagagatggg ttgtggtgaa
     3601 ggaacaacac gtctataccc tcttccagca tcttcaacaa atgtaaatcc tttttctttt
     3661 tgaatttgtt cagcttcttc tttgttgtaa aataacccaa ttggttttga aggattgtta
     3721 aatgccggat cattttcgtc aacttcaact tgtgtcacta gtgttaccac ttgtttatcc
     3781 attccaatag aatgcaattc gttttgtaag ctttcttgta attgatagcc gatgtaagct
     3841 tgactcattg cgccacattc agcaaatgga aatgccggac cttggttgtg ttctgcagca
     3901 tagttaagtc ccaaattaat gcttccaacc tgtggcccat taccatgact aataacaatc
     3961 tcatgtcctt ttgttattaa tcctactaat gatttcgcag tatttttaac aagcttgagt
     4021 tgttcttgag gtgattttcc taaagcattc ccacctaatg ctactactat tttcgccatc
     4081 atattcactt ccttatatca tttaaaattc acccaatgta gcaaccatga ctgctttgat
     4141 tgtatgcatt ctgttctcag cttcttggaa tacaactgaa gctttacttt cgaatacttc
     4201 atctgtaact tccatttctc gaataccata tttttcaaaa atttgttgtc caattttggt
     4261 atcagcattg tggaaagatg gtaagcaatg ctcaaaaata acatttggat taccagtttt
     4321 atccatcatt tctttattta cttgataagg tttcaataat tcaagtcgtt ctttccatac
     4381 ttcatcaggt tcacccattg atacccaaac gtcagtgtaa attacatccg aaccctttac
     4441 accttggtca atatcatctg tgattaatat gttgccacca ttttcagcgg caatattttt
     4501 acagcgattt aataattcat ctgttggatt taattctttt ggacaaacta aatggaagtt
     4561 catacccata atggcagcac cttgcattaa tgcatttgca acgttattac gtccatctcc
     4621 aacatatgta aagttaatat ctgcataatc ttttttcaaa acttcttttg ctgttaagaa
     4681 atcagcaaga acttgagtag gatgatcttc atcagttaaa ccattccaca ctggtacgcc
     4741 tgaatattca gctaaagttt ctactgttct ttgtgaaaaa ccacggtatt caatgccatc
     4801 atacattcca ccaagcacac gtgcagtatc tttagttgtt tcttttttac ccatttgtga
     4861 tccagttggg cctaaataag ttacattagc accttgatca tgcgctgcaa cttcaaatgc
     4921 acatcgcgtt cttgtagaat ctttttcaaa taacagtgca atatttttat tttttaacat
     4981 aggcttttca gtgccaatat atttagcacg ttttaaatcc tcggagagtg ttaataagaa
     5041 ttctacctct tgtcgtgaaa agtctaataa agttaaaaaa cttctgtttc gtaaattttt
     5101 cattaataac aactcctttt attttagtta cacctaaatt gtaagcgcat acaaaataaa
     5161 cacaacctac tattaaaatt tgtaatattt tatcaataat taaatgaaca ttttattaat
     5221 attaaattta agtagtagga aataattaaa ataagtacta catttaaagt ataactattt
     5281 ttcaagtagt tagaaaattc aataaataac taatttaacg caattaatat cataattagc
     5341 atcagtttta atttattagc actcttttca ttttataatt ggcaatgttc caatgcttaa
     5401 aaatgaaaaa ttttacttaa atcacacgtt caactgttta tgtttattta acatagtgtt
     5461 agcttttatt taattccgaa tcggtgcaat accttatata ctatgggtaa ttcacgcaaa
     5521 ggagattatc atatgaaaaa gaacatcacg aacaaaattg ttttatcaac agcattgtta
     5581 cttttaggaa ctacatcaac acaaccgcct aaagcaccaa tcagtttttc atctgaagca
     5641 aaagcataca atataagtga aaacgaaacg aatatcaatg aattaatcaa atattacacg
     5701 cagccacacc tttcattttc tggaaaatgg ctatggcaaa accctaacgg taccatacat
     5761 gcaacattac aaacgtgggt ttggtacacg catattcaag tatttggtcc tgaaagttgg
     5821 ggcaacatta atcaattaag agataaatat gtagatattt ttggtaccaa ggatgaggat
     5881 actatagaag gctattggac ttatgatgaa acctttactg gaggtgtaac accagcagct
     5941 acttcatctg ataagcctta taaattattt ttaaaatata aggataaaca acaaactatg
     6001 attggtggac ttgagtttta tcaaggtaat aaaccagtaa taactttaaa agaactagat
     6061 ttccgcgtac gtcaaacttt aattaagaac aaaaagctat ataatggaga atttaataaa
     6121 ggtcaaatta ggattacagg tggtggtaat cattatacga ttgatttgag taaaaagtta
     6181 aaactaactg acacaaaccg ttatgttaaa aatcctcgac atgcagaaat tgaagtcatt
     6241 cttgaaaaat ctcattagta tcattcattg agtgaataca aatagactga atgatttaat
     6301 tgatttctct ctttttataa acttattatt cctcgcatat aacattcctc gtaaaggaga
     6361 ttttctaatg aaaaacaacc tcacaaaaaa aattatttta tcaatagcat tgactatgat
     6421 aggcacagct acaagtcaat cccctcattc accgattagt atgtcatctg aagcaaaagc
     6481 atacaatata agtgaaagcg aaacgaatgt aaatgaattg actaaatatt acacacaacg
     6541 ccatttaaca ttttcaaata aatggctatg gcaaaaagat aatggtacaa tccatgcaac
     6601 attgttacag ttatcatggt ttagtcatat tcaagtattc ggccctgaaa gttggggtaa
     6661 tattaatcaa ttaagaaata aatatgtaga tatttttgct ttaaaagatt atgaaacctg
     6721 gcgcacttat atgttagccc aagaaacatt tactggcggt gttacaccag tagcaaaacc
     6781 tagcgataaa cattataaat tgaatgtaac atataaagat aaagcaggaa catttattgg
     6841 cgaatatcaa ttttatacag gtaataaacc agttttaact ttaaaggaag ttgatttccg
     6901 aatccgccaa acactaatta aaaacaaaaa attatataat ggtgattata ataaaggcca
     6961 cattaagatt acaggtggca gcaacaacta cacaatagat ttaagcaaaa gattaaaatc
     7021 aactgatgca aatagatatg ttaaaaaccc tcaaactgca cacatcgaaa tcctccttga
     7081 aaaatctagc taacatcgaa atcgaagtaa acagaaataa gcacaatgtt tacattgacg
     7141 ccgttcattt ttaaatttgt taattctcgt attgatgatt acgcagaaag gagaagttcg
     7201 aatgagaaag tgcattatga aaaagttaat tttaatgaca acattgttat tattaggaac
     7261 aacagctaca caaacctcta attcgccatt aaatgtttct acagacgcta aagcttatca
     7321 tattggccaa gacgaaacga acatcaatga gttaattaaa tattacacac aactgcctct
     7381 cacattttca aatagatggt tataccaata tgatgacggc aacatttatg ttgaatttaa
     7441 gcgttattct tggtcagcgc atatacgatt atggggtgct gaaagttggg gcaacgttaa
     7501 tcaattaaga gaccgctatg tagacgtgtt tggtttaaaa gacgaagaca ctagccagtt
     7561 atggtgggta tatcgtgata catttacagg tggtgtaaca cctgctgcat caccttctga
     7621 taaaccatat agtctttttg tccaatacaa agataaacta caaacaatta ttggtgcaca
     7681 taagatgtac caaggtaata aaccaatatt aacattgaaa gaaattgatt ttcgagcacg
     7741 tgaaacacta attaaaaata aaatattata tcatgaaaat cgcaataaag gtaaacttaa
     7801 aatcaccggt ggcggcaatg actttacaat tgatttgagt aaaagattac attctgatct
     7861 tgcaaatgtt tatgttaaaa atcctcaaaa aataacagtc gaagtcctca ttgattaaat
     7921 aaggtcgtat gatttatatc gtaaatacta aggataaaca atatttgagt aatactacaa
     7981 aatgtctcaa tagtacttac acaatcatta atttagtaca cagaatatta aatgcgattt
     8041 ggagtctaat agaaatgcca agtcattctc tgggtttggg cccgccccaa cttgcattgc
     8101 ttgtagaaat tggtaaccca atttctctat gttggggccc caccccaact tacattgtct
     8161 gtagaatttc tattcgaaat tctttttgtt ggggcccctg tctctaattt aaagtcttgt
     8221 tataatcgca tttttgtttg gtcaatgtct gtctatataa tattg
//
LOCUS       DAMZPP010000123         8246 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMZPP010000123 DAMZPP010000000
VERSION     DAMZPP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.123"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..197)
                     /locus_tag="POH73_002192"
     CDS             complement(<1..197)
                     /locus_tag="POH73_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888119.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLEL"
     gene            complement(217..1362)
                     /locus_tag="POH73_002193"
     CDS             complement(217..1362)
                     /locus_tag="POH73_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDI6888120.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            complement(1386..2123)
                     /locus_tag="POH73_002194"
     CDS             complement(1386..2123)
                     /locus_tag="POH73_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDI6888121.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            complement(2107..3294)
                     /locus_tag="POH73_002195"
     CDS             complement(2107..3294)
                     /locus_tag="POH73_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI6888122.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            complement(3310..4971)
                     /locus_tag="POH73_002196"
     CDS             complement(3310..4971)
                     /locus_tag="POH73_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDI6888123.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNK
                     EYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            complement(4968..5312)
                     /locus_tag="POH73_002197"
     CDS             complement(4968..5312)
                     /locus_tag="POH73_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888124.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            complement(5443..5742)
                     /locus_tag="POH73_002198"
     CDS             complement(5443..5742)
                     /locus_tag="POH73_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6888125.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKHKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            complement(5974..6390)
                     /locus_tag="POH73_002199"
     CDS             complement(5974..6390)
                     /locus_tag="POH73_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6888126.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            complement(6418..6618)
                     /locus_tag="POH73_002200"
     CDS             complement(6418..6618)
                     /locus_tag="POH73_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HDI6888127.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLEEYRIKLKRMNNDIKK"
     gene            complement(6618..7268)
                     /locus_tag="POH73_002201"
     CDS             complement(6618..7268)
                     /locus_tag="POH73_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888128.1"
                     /translation="MNEQIIGSIYTLAGGVVLYSVKEIFRYFTDSNLQRKKINLEQIY
                     PIYLDCFKKAKKMIGAYIIPTEQHEFLDFFDIEVFNNLDKQSKKAYENVIGFRQMINL
                     SNRVKAMEDFKMSFNNEFSTNQIFFNPSFVMETIAIINEYQKDISYLKNIINKMNENR
                     AYNHIDSFITSEYRRKINDYNLYLDKFEEQFSQKFKINRTSIKERIIINLNKRRFK"
     gene            complement(7427..7576)
                     /locus_tag="POH73_002202"
     CDS             complement(7427..7576)
                     /locus_tag="POH73_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6888129.1"
                     /translation="MVKEILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            complement(7579..7779)
                     /locus_tag="POH73_002203"
     CDS             complement(7579..7779)
                     /locus_tag="POH73_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888130.1"
                     /translation="MPKKTVTIDVDENLLVVASNEISELLYEYDSELMSADEDGDNRD
                     IEEKRDALKQAIQIIDKLTWGV"
     gene            complement(7754..7942)
                     /locus_tag="POH73_002204"
     CDS             complement(7754..7942)
                     /locus_tag="POH73_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDI6888131.1"
                     /translation="MTQYLVTTFKDSTGQPHEHFTTARDNQTFTVVEAESKEEAERKY
                     EAQVKIRRDGDAKENGND"
     gene            complement(7939..8184)
                     /locus_tag="POH73_002205"
     CDS             complement(7939..8184)
                     /locus_tag="POH73_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888132.1"
                     /translation="MSDMLEIFLIGFGVYLFYRIAIIFLKSKKTIHTNIYEMLMLATI
                     FMISTFAYKHQKTHILIAFLVMFFMSKLKQVQGSYEE"
BASE COUNT         2394 a         1643 c         1039 g         3170 t
ORIGIN      
        1 agttctaata attcttgttt tgtcagctta tctaaaggta cgatataaac tttgtcgtac
       61 ttatttttga tttgatttgt caacaattca acacgaggat tgttataccc ttcagctgga
      121 tacaactccc ctactttgta cttgtgttga ttgtgctcta tgtctttaaa agctctaaca
      181 actttaaatt tcaccatttt atcacctcat aaaattttat agtgtttctt cggtaccttc
      241 taaagctggc ttatgtcctt ttaaatctaa tttccaaaca gcagcaactt tattatcttt
      301 cgctttgccg taagcaaatt gttttgcagt gtataaatcc atatcatcta acgcaagtgt
      361 ttctttaaat ttctgaacat taataccacc agctaaataa ccatcatata aacctttaac
      421 gtacgttaaa accttacctg cttcttggac tgtagactcg ataacattca aattaaatgg
      481 taaagcagta acatatacgc catttgcatt taaatgtgta tactgtgctt gaacctcaaa
      541 agcatcggac ggattaacaa ccattgttac attaccttta accgctactg atttaccttt
      601 ctcgttagtt gagtggtatt taaacacttg cgtcaattca ttaactgtag cgcgcggatt
      661 agcaaatgta agcgtacctt gttcttcttt ctctggataa gcaccctcag ttaccgatac
      721 acctttttgt acttgacggt ttaagccgat tggttggtct ttaccagtac cttttaagaa
      781 cgcagtttca agcgccactg caaatgcttc ttcgatttga acacgaacaa atctttcaat
      841 ccacgcagga ccaaaatcat ttaaatcttt tggtaaaaca acaaacgctg tcaatttatt
      901 ttgaattgct gtttcttcac tgaacgcagc atctaattga cctttaattt caccatagat
      961 tttaccccaa acggctacgc cagaagtttc agattttaag aacttcaaac gcaaaccagc
     1021 gtttttaata cctaaatcag ctaataacgg atgattcgtc gtcaaatctt caaaaattct
     1081 atcaattgtt tcttctggca aaagtttttc ttctttatag ttaacgtttt tattgatatc
     1141 catgaagaaa cttctttggt ttgcactcaa agattgtgct gatttaggta aactagaaac
     1201 tctttcagct tctgcttttg cttgtaattt agtttcttca aatagttggt taatcatgtc
     1261 accgtacaat tcattttgtc tttcttgcgg ttcaccgttg tttactgcat taataaattc
     1321 gtttttcgca tttgcgaatg tttccgataa atttatagtc attttatgac ctcctatttt
     1381 ttgtattaaa aaaggaatct tgaaaatcca tttgctgata ctttactatc tgcaacatcg
     1441 atttctgatt ccttttcttt catatttatt ttttcaatta ctttatttgc tattgcgtca
     1501 atatcaatgt taacctctgg cgttttactt accaaagctg ttacacgatt taatacatct
     1561 ttcgataaca cttgtgtatt gcttgctaca atttgcatat tgtcgttttc aaacatttta
     1621 ctatccgcaa aaccttgttc aatggcttca tcagcattta gccacgtttc cctagccatc
     1681 atttctacaa gttcttgttt gtttttacca gctctaaccg catatgcctc agccattatt
     1741 tgaccaacat gttctaatgt ttctgcagca tgatttagat ctttcgcttc tccttgcgca
     1801 atacttgaag gattgtgaat catcattcta gcaaccggac tcatttcgat gtggtcacca
     1861 gccattgcga taagcgatgc cgcacttgct gctattgctg tgatacgaac attcactttg
     1921 cctttatgag ctcttaaatg tgtatatatt tcactaccag ctactaggtt accaccattt
     1981 gagttaatta taatatcaac atcttcatca ctaaattcta gttgtgttaa aacatcttta
     2041 ggacaagtcg aatccatacc aagcatttcg taaacccatt tatcttcgtt ggaaacgatg
     2101 acgcctttaa tctccgcttt catcttcatc accacctttc aaagtgtttt catctttttc
     2161 tttttcatca ttttcaccac tgttagcttt ttcgtagttt ttagtaatca ggtattcgtc
     2221 taattcagga ttgtctgatg gttcttcacc taacataatc cgcacctcat tccttgtaaa
     2281 tgaaccagaa cttacaagtt tgtcaattgc ttcagcatat tgaagtgggt cttttttatt
     2341 cacaccgaca atttctattc ttgtatcttt caaatacatg ctttgtgtta tgagtttcgc
     2401 gtttaattcg ttctgaatct tttttaataa aggtgttaaa cagaacttct caaatacaag
     2461 cgtgtttttt tccaaatcag ctgtttctcc gtaaatcaaa cctggaggta taccaatcat
     2521 caacgcaaca ttttttattg catctctcat tagctcactc aattcagaaa aaggcatgtt
     2581 actattctta ccaccattag ataattcctc ataatcaaaa ccttctatca aaggcgcgat
     2641 tgctagttga tttttattaa aagtattgaa taatttattt gtgaacgctt gtaatttttc
     2701 tatattcttt tcgtcatatg cgctagaggc agatttcaaa atccctctta tttgatagtt
     2761 ttttaattgt gcacctatca ttcttccgaa tattttcccg taatcttcga atagactttc
     2821 tacaaagtgt gtcactttat tgttgttgta ctttaaatat atgacctctt gcattgtgaa
     2881 agtacgttga taagtataat ctttaaccgt tacatctttg aatatatcat catacaaagc
     2941 gtactcttct ctgtaaaagc tatctgcgat aagtaattct ttgctgtcac ttactacgat
     3001 taaaacctcg ttatcataaa ttagtttata tataacttgt tgccaaaaac tatcgcttga
     3061 taagtcagta tttggtttta tatttaactt gtagtaaaca tcattctttt gaattctatt
     3121 accttccaat actttaaaat gactttgagc gacagctcgc gcaacaaatt caatacaact
     3181 atcaatcgct aaacgtttca catacgcttg ttgtgataga tcttctatca tatctaaatc
     3241 aagcatatat gttatatctt tcctagtttt aaatatcttt tctagaatac tcatgtctca
     3301 cctcctctat tagaaatcta tactcattaa tgcatcaagc gctttagaca tgtctttgtc
     3361 tactatatcg tctgctctat ataatgcgtg aacaaaagcc atgaacccat cggtttttct
     3421 tctattttca tcttttttaa tatattcttt attaccatcg ggtttaacct ttactgcaac
     3481 attattagta aaccaacgca tcaaaggatt gtctccatat attacgttat gtttcgcaaa
     3541 cattgtatcg atacgtggtg caagtaatcc atgtattgct tttggatttc taagtacttc
     3601 aagttttatg ccagcatcct caaacgcacg tcttacaata tcagttctat aattatcagc
     3661 tatgactttt tcaagcccat atttttctct agcctttaaa aaccaatcaa ctatatattc
     3721 aatttcaatg acatcatcat cgacaatggt caataatccc attttttccc attctttaat
     3781 aggaggttct aatttgacat catccaaaaa cccttgtctt acaaacgaat gtcctaacca
     3841 aatgtaatca tcgttttttc ggaataatag ccctacactt gcaaaatctc gaatgtttgc
     3901 aaagtctaaa ccaccaatac acatttgatt atctaaattt ggtatctctc tattagtcgc
     3961 tagtatttct ttccatggtg ctattacttt ttcaaggtca acttcaggca aattcattcg
     4021 cttagtcatg aattcgggct tatttgaacg gttgaatggt aaatcgttat attcttcttc
     4081 aatcgtactt agcagtgttt tagcgtattc tgataacggt ttatgtaaca ttgggttcgc
     4141 cttttcccac gtctgtctgt catcaacttc ttttggatcg tctaacttac aataaaaagc
     4201 aaacaatcta ctatttttaa ccttgccact taatacactt gcaattttgt gcttcattgc
     4261 atcgatataa ccctctctaa caaaaccatc agtacttata taaaacgttc ttctattttt
     4321 ctttttacct aatccaccac gtttgacgtt taccatttca ggaccaaaga aataatgaat
     4381 ttcatcaaaa ataacacacc cctcacgtcc accgtctttg gtttttgtgt ttgatgtgtt
     4441 atatcgaata accgatttag ttgcacggtt tattattttt gctttactaa cttcataagg
     4501 agcttttggc gttttacccg tcttatttcg tttgttatcc attaaaacgg ttctgatttc
     4561 atcaaacgat gtttttgctt gatcttcact attagcaaca atggagatgt gatattcttt
     4621 aactccgtgt aagggcgtag aaagaaaatc actaatagca cttattagac cgtttttccc
     4681 gcctccacgt cccatgaaaa tagcaaattc tgtaaagaaa gcttcatctg tatttttatc
     4741 tataagaaat atattagcta tgataaacct ttgaaatggt aatgttggaa aataccattt
     4801 ttcaataaat ttgatacaat cctcgatttt ctgttcatca aaatatacat catctcgtga
     4861 atatatatgt ttttgtagat aattaaagag atcaattctt tctttattta aaattatctt
     4921 tccttgtttc cacaaattta tatattcatc aacgtattta ttactaatca taggtaatca
     4981 tcagatggcg tttctgtgtc ttctttctct tcgggcaata aatccgataa ttgtttgatt
     5041 attttttgat atgcagcatc tctagcatta aatagtttgg ctactggtct ttccctttca
     5101 tatggtggcg ccttttcaga ttgagtaaat aaatcatagt cacctttttc ttttatgtct
     5161 tcccacatgt aatcaagcat tacacgtagc cttgctgctt gaataattaa accatcaact
     5221 acttttaatt tattgctagg tatgtcttta tataatactt gcagcctttc tttttcttta
     5281 agcactaagt tttcatcaac tataatctcc atttcatcac ctgccttaaa atggttataa
     5341 gagggggggt tatacatgga tttttaaaat tatcgcgaag tcgagcccct gcccgttccc
     5401 caagtatttt gatcgctttt gatttttttg acccgggggt atttaccatt tttcgtcttt
     5461 ccatttattt tcttttttta taaatctctt ttcttttttg ttgtgacatt taatacacag
     5521 tgtttctaaa ttgtttaagt catgagcaaa ctccggatga tgttctagcg ataatatatg
     5581 atctacatcc aacgacttat gtttgctttt gtcatatgtc gttaacttgc cgtctctctt
     5641 acattgttga cattcataat tatctctttc tagtactctt tttcttgttg tttgccattc
     5701 tttagactta tagaatcgta tacgttcgtc tttagtcatc ataatgtttc accttatata
     5761 acttaagtag tatcaagacg catctatact tgatgtgtag taatgtattt acaattagtt
     5821 tgaacatgtt catacctcat aaataaaaag acacatcaca tagtaatgcg cctcttgttc
     5881 atgcgtcgta ttagcattta ataactttaa atattaatct gatactaaca taataaactg
     5941 ttttaatgcg gacttacata gggtaaaagt ccgctacaca taaccaatat actttgctaa
     6001 cttatcgatc agtgcattcc ttctacgtaa tatacttgtc ttacttgtac caaagtaatg
     6061 tgctatatct tcccattcat aacaaccaat aggacaatcc caatatctaa accttaataa
     6121 ctcaagcgta tcctcatcac tttcatctat caatctatct acaccgttaa ctatatttct
     6181 taatgtattg tacctgttat cactaaactt ctttattgca catcgttcaa tcggattacc
     6241 cggcaaatta cttttgccag ctcccgcatt atctggttca tgactttcaa gtaattcata
     6301 ttctcgcatc ttcaactctc ttcgatagtt atcgatgtgc tgaatgtatt cttcaagctt
     6361 tttgatatcg tgtttctcaa tctttatcat tcaatgcaat acctccgata atataaatta
     6421 ctttttaata tcgttattca ttcgctttaa tttaatcctg tattcttcta acccgttgta
     6481 tcctttagtt ttaactactt catcaagtag ataatcattc atatatctga gtgcttgtat
     6541 ctctcttgca cgatcactat taatactgat acaaactaat agcaatatag caaatacaat
     6601 agtcatagta atccacatca tttaaatctc ctcttgttta aattaataat aattctttct
     6661 tttatcgaag ttctgtttat tttaaacttt tgactaaact gttcttcaaa tttatcaaga
     6721 taaagattat aatcgtttat ttttcgtcgg tactctgaag tgataaaact atcaatatga
     6781 ttataagctc tattttcatt cattttatta attatatttt ttaaataaga tatatctttt
     6841 tgatattcat ttataatagc aattgtttcc ataacaaaag aaggattaaa aaaaatctga
     6901 tttgtactaa attcattgtt gaaactcatc ttaaaatctt ccattgcctt aactctattt
     6961 gataaattaa tcatttgtct aaatccaata acattttcat acgctttttt actttgctta
     7021 tctaaattat taaagacttc aatatcaaaa aaatctaaaa attcatgctg ttctgttgga
     7081 ataatataag ctccaatcat ctttttagcc tttttaaaac aatctaaata tatcggatat
     7141 atttgttcta aattgatttt tttacgttgt aagttagaat ctgtaaaata cctaaaaatc
     7201 tctttaactg aataaagcac aacacctcct gctaaagtat atatgcttcc tattatttgc
     7261 tcgttcattt ttatctacct ctttataata ttttctgaaa aggaatctat aattttatac
     7321 ataccaaaaa ttcctaatgc atttattatc actccatcat caataatata tatggatatt
     7381 aagaaagcaa acagcaaaac gatcaaatca taaataaata ttctcattat tcactcacct
     7441 ccgctcgaaa gacgtaatca ctcggcgcct ctacatcatc attagccgtc atcataatat
     7501 atacttgctc agttacatac ttacctaact catacatcgc tagtaagaat aatagtctca
     7561 aaatttcttt aaccaccact aaacacccca tgttaattta tcgataattt gtatagcttg
     7621 ttttaatgcg tctctttttt cttcgatatc tctattatcg ccatcttcat cagctgacat
     7681 taactcactg tcatattcat ataatagttc tgatatttca ttactagcta ctactaataa
     7741 gttttcatct acatcaatcg ttaccgtttt ctttggcatc tccatctctc cttatcttaa
     7801 cttgtgcctc gtatttgcgc tcagcttctt ctttactctc tgcctcaaca actgtaaacg
     7861 tctgattatc tctagcagta gtaaaatgtt catgtggttg tcctgttgaa tctttgaatg
     7921 ttgtgactaa gtattgtgtc attcctcata gctcccttga acttgtttga gcttactcat
     7981 aaaaaacatt actaaaaatg ctattaagat atgcgtcttt tgatgtttat aagcaaatgt
     8041 agatatcata aagatagtag caagcattaa catttcatat atgtttgtgt gtatagtctt
     8101 tttactctta agaaaaataa ttgctatgcg ataaaagaga taaacgccaa accctattaa
     8161 aaatatttct aacatgtcgc tcacttcccc aaaacctcct tgactcgatc taagatgtct
     8221 ttatactccg ctacttccga agcctt
//
LOCUS       DAMZPP010000124         8132 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMZPP010000124 DAMZPP010000000
VERSION     DAMZPP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.124"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..2027
                     /locus_tag="POH73_002206"
     CDS             <1..2027
                     /locus_tag="POH73_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDI6888133.1"
                     /translation="KILDKYKTFERDLADSFLGIGGERLTENELAYIIRKPFISNATH
                     DVQEESSRRAITDIYNAVIDPTRLSVLEVENDNEKFYTTTVVVDDFPLDMEYTHLFEK
                     AQNMPFPVECHVKAKIINNEKVKKKFDRAKINHKQQAKEKQDTGDSASEDVQDNLFVL
                     NQMEREVTGSGVFIEWVFMFVIKSDDYRDCKRKAKRLMKRLKETQIYAVNPLADQLQL
                     FYQCLHGNDLFINKYWLQRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREE
                     AVASSRDIILFSLLLAAKGIKGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQM
                     LYWDPKSEFAEWFDRVTESKEMQEKYPLFINHLKTFKYVTLNYEDSTNYGCLDPIVFL
                     DGIEANEMLKSVFDEIKSFENYHHIETAIYQAISDTVEERENGKKVGSLNVIEKLQNN
                     EDEHVKNAGDLLFEKTQNNILKLVFSDGTNPALNIQEKATILQVKGLDMPKADDDTSS
                     YSTSEKNGITLMLLIGKFLEKFGSRRDVQTTIFIDEGWAFSASRQGKKVGKRIKRTGR
                     SENNSLVFITQSVKDKADDDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEML
                     KDLKKGQCIFSDFYGRVGKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAM"
     gene            2081..3439
                     /locus_tag="POH73_002207"
     CDS             2081..3439
                     /locus_tag="POH73_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDI6888134.1"
                     /translation="MAMNEIALFKGVRIQPYQKYIDFMFAGVLAFIFLVYRIYILFID
                     YKSVNKKLDIWTLFAYFKPHLLYLLIGTAIIFVICKLIAQLLIRFKTKDLAEMIETQG
                     FHNRTVIKKTTEYIPIDYKQTEYDYYPTMFYKRGKKTFVIHIKKDGSRFQDNYLELET
                     IIEPMFNSELVEKKHIGRYLRYEFMPLKYKKRIVMNGEQESNTIFYDTKIAITHQITW
                     DFVKAPHGLVTGITGGGKTYFLFYVIRELFRRHSEVRLLDPKVSDLSFMKRVIGDDKV
                     ADTKGQILKQLREANNEMEERFRLMNDSSDYKIGNDFRNFDMRPYFIIFDEVTAFTST
                     LDKKELQEMNDYLINIIMKGRQAGVFMFLTAQRPDADVIKGNVRDQLGLRVSLGNLSN
                     DGYRMTFGQTDKEFQTIHDSDIGRGYISILGQYNEPILFDAPLMEQYDFVEDVKQILN
                     KE"
     gene            3444..5291
                     /locus_tag="POH73_002208"
     CDS             3444..5291
                     /locus_tag="POH73_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888135.1"
                     /translation="MKHKSVYLLCFLILPILLLTSVSAYAVSNPVGEPSHAVKPKIEK
                     YDLSHYRSIYSEKGDWNPFGQEEISRQINNVSDFFFSMTKILAGVTDYAIENLFQLDV
                     INDFADKIGDFVGDIYQKLLSNLAITLFIIVCFNAFIIFSVQGNAREALKRAFLIMCL
                     IGFGVGILANAGNIIRGTNNIGKDLNNIIMNSTSSIDGNIDYIHENSGMNHIRNQLFD
                     MTIYRTYLVMNYGTVDEKEIKAKGKDRIDNILKLDYDKKSEKELDDIVKDEVEKKNNK
                     YMTQGYVFQKLAISVIGFIITLFMSIVFLSISFAKLIFSTFALFLFLFLVFSWIVSFI
                     PGFELSVFSAFAKTLGYIILSACMTFLFVIVGLCIKLANSFIDPDSQNAYFLNSIFII
                     VILFVMYKKRAQIINFVSRGNISFSPSAIGAGVMNRTQERFNKIRHEQAQNKKAKREN
                     QRDEPAPPLQNDNDLRRRQQYKPMPLFINKENQKNGNKRREQQESMNGNDVKSASVES
                     NANNYSKQPQKASQQEHQVRETRQRKDIQRSPQVVNQPLNNENHSINRKEQKSVQTAY
                     DTDVQKRQIQNATQNQQSRQSGNRNQPITRNSQSKDRLKEQKDINKHGK"
     gene            5281..6327
                     /locus_tag="POH73_002209"
     CDS             5281..6327
                     /locus_tag="POH73_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6888136.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFTFPVIVMF
                     LLAPDSNKDKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIEDGLNAGAKNLYDVYIS
                     KGLDTPKKIGPKYAPVGASNDPNNMNARWIPTVEKIMKDLGGSEAKTSCSNGKGKSIK
                     FNGKLPHWSNDDPGKGNLYTAGQCTWYAYGMRQKMGKPVSTYWHDAHKWNDRAKAEGY
                     KVDKNPEPGALFIAEQGAGGHDGHYGHVAVVIGVSDGGKTFRITEMNWEGAFKVNERT
                     LKMTDGYSFIHDKE"
     gene            6334..6924
                     /locus_tag="POH73_002210"
     CDS             6334..6924
                     /locus_tag="POH73_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888137.1"
                     /translation="MKQKIIMFTLVTVILFCAVLIGYQIPKQQVKMKQNQIEDLQEEQ
                     RILRDKNGELNKLVKRQSKTVISDEEKQIREVSSNFVKQMFEMKKDSSFKSKAPQIKP
                     LVTKDYYDTLFKDSKDKYDLYDDITVNDIHVYFDTYDPKKDSYKVFVQFDERIETDGD
                     DKIEHRQTSAQLDLVRTAEGWRIDNLKRFNLKPLGR"
     gene            6980..7336
                     /locus_tag="POH73_002211"
     CDS             6980..7336
                     /locus_tag="POH73_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin-like fold lipoprotein"
                     /protein_id="HDI6888138.1"
                     /translation="MRRWFVLILGLVILLSACGQKYDKEIDEVTKLEKESIQDVKNTK
                     KYKNVERSKSYYKIYNDGEVIIMTYMPFKDSNTKVSRVYKINQTSDQYEEDSNIDPEK
                     FEKDNKPVYEENNMKK"
     gene            7445..7948
                     /locus_tag="POH73_002212"
     CDS             7445..7948
                     /locus_tag="POH73_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6888139.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPRQI
                     ASQIEKDTGHYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGSRVI
                     KYRLKKY"
BASE COUNT         3011 a          905 c         1543 g         2673 t
ORIGIN      
        1 ataaaatttt agataagtat aaaacatttg aacgagattt ggctgatagt tttctaggta
       61 ttggaggcga acgtttaact gagaatgagc tggcttatat tattcggaag ccatttatat
      121 caaatgcaac acatgacgta caggaagaat cttcaagacg agctattaca gatatttata
      181 atgcagtaat tgaccctact cgtttgagtg ttttagaagt tgaaaatgat aatgaaaaat
      241 tttacacaac aacagttgtt gttgatgatt ttccgttaga tatggaatat acacatttat
      301 ttgagaaagc tcaaaatatg ccttttccgg ttgaatgtca tgtaaaagca aaaattatta
      361 ataatgaaaa agttaagaaa aaatttgacc gtgcgaaaat aaatcataaa caacaagcaa
      421 aagaaaagca agatactggc gatagtgctt cggaagatgt tcaagataat ttgtttgtat
      481 tgaatcaaat ggaacgagaa gtgactggct caggtgtatt tattgagtgg gtatttatgt
      541 ttgtaattaa aagtgatgat tatagagact gtaaacgtaa agcaaagcgt ttgatgaaac
      601 gattaaagga aactcaaata tatgcagtta atccattagc tgaccagtta caattgtttt
      661 atcagtgttt acatggtaat gatttgttta ttaataagta ttggttacaa agaactacgg
      721 caacaggtat agcagaaaat ttatttggtg tgtctcaaag tttgggaaca aaaacaggtt
      781 tttatatagg gcgtattgat aaatttattc gttctgtttc acgtgaggaa gctgttgcaa
      841 gttcaaggga tataatttta tttagtttat tgttggccgc aaaaggtatt aaaggggctg
      901 tcagtgatag tccgcatgtt ttaattactg gtcaaactgg taaaggtaaa tcatttttag
      961 caaaattatt atggatttat acatcatttt ttaaaggtca aatgttgtac tgggatccaa
     1021 aatctgagtt tgcagaatgg tttgacagag taacagaaag taaggaaatg caagaaaagt
     1081 atcccctatt tattaatcat ttaaaaacat ttaaatatgt aacattgaat tatgaagatt
     1141 caaccaatta tggttgtcta gaccctattg tgtttttaga tggtattgaa gctaatgaga
     1201 tgttgaaatc agtgtttgat gaaattaaat cttttgaaaa ttatcatcat attgaaacgg
     1261 caatttatca agctatttct gatacggttg aagaacgtga aaatggtaaa aaagttggtt
     1321 cattaaatgt aattgaaaaa ttacagaata atgaagatga gcatgtaaaa aatgcaggcg
     1381 atttattatt tgaaaaaacg caaaacaata ttttaaaact tgtttttagt gatggtacga
     1441 atccagcttt aaatattcaa gaaaaagcaa ctattttaca ggttaaaggt ttagatatgc
     1501 ctaaagcaga tgatgataca tcaagttatt caacaagtga gaaaaatggg attacattaa
     1561 tgttattaat tggtaagttt ttagagaaat tcggttctcg tagagatgtt caaacgacta
     1621 tatttattga tgagggttgg gcttttagtg cttcaagaca aggtaaaaaa gttggtaagc
     1681 gtataaaaag aacgggacgt tctgagaata attcacttgt atttattaca caatcggtaa
     1741 aagataaagc agatgatgat ggtggtaact ttggttgtca ttttgcattt gatgaaaaag
     1801 atgaacgtga ggatatttta aaatctttag gcttagaata ttcaaaagaa tctcctgaaa
     1861 atatggaaat gttgaaagat ttgaagaaag gtcaatgtat atttagtgat ttttatggac
     1921 gtgttggaaa aatggttgta cattgtccat ttgaggaaat gactgaagca tttagaacac
     1981 aagaagattc tgcaagctct aaagctgaag aaaaatttgc gatgtagttt ttagcacata
     2041 caaaatagta tgtgcttttt attttaaagg agtggatttg atggcaatga atgaaattgc
     2101 tttatttaaa ggcgtacgta tacagcctta tcagaagtat attgatttta tgtttgcagg
     2161 tgtattagca tttatttttt tggtatatcg tatatacatt ttgtttattg attataaatc
     2221 agttaataaa aaattggata tatggacgtt gtttgcatat tttaaaccgc atttattgta
     2281 tttattaatt ggtacagcaa ttatatttgt tatatgtaag cttatcgctc agcttttaat
     2341 tcgttttaaa acaaaagatt tagctgaaat gattgaaaca caaggttttc ataatcgtac
     2401 agtaatcaag aaaacaaccg agtatatccc tattgattat aaacaaactg aatatgacta
     2461 ttatccaacg atgttttata aacgtggtaa aaaaactttt gttattcata ttaaaaaaga
     2521 cggttcgagg tttcaagata attatttaga gttagaaacg attattgaac caatgtttaa
     2581 ttctgaatta gtagaaaaaa agcatattgg tcgttatttg agatatgaat ttatgccact
     2641 taaatataaa aagcgtattg ttatgaatgg cgaacaagaa tcaaatacaa ttttttatga
     2701 tacaaaaatt gctattacgc atcaaattac ttgggatttt gtgaaagcac ctcatggatt
     2761 ggttacaggt ataaccggag gtggtaaaac atatttttta ttttatgtga ttcgtgagtt
     2821 attccgtaga cattcagaag tacgtttatt agatccaaaa gtttcagatt tgagctttat
     2881 gaagcgcgtt attggtgatg ataaagttgc agatactaaa ggtcaaatat taaaacaatt
     2941 gagagaagct aataatgaaa tggaagagcg ttttagatta atgaatgata gttcggatta
     3001 taaaatcggg aatgatttcc gtaattttga tatgcgcccc tatttcatta tttttgatga
     3061 ggtaacggct tttacttcaa ctttagataa aaaagaatta caagaaatga atgattattt
     3121 aattaatatt attatgaaag gtcgtcaagc tggtgtattt atgtttttaa cagcacaaag
     3181 accagatgca gatgtgatta aaggtaatgt gcgtgatcaa ttagggttac gtgtgtcttt
     3241 aggtaatttg tcaaatgatg gttatcgtat gacctttggt caaactgata aagaatttca
     3301 aacgattcat gatagtgaca tcggtcgagg ttatatttct atacttggac aatataatga
     3361 accaatttta tttgatgcac ctttaatgga acaatatgat tttgtggaag atgtgaaaca
     3421 aatattaaat aaggagtgat ttgatgaagc ataaaagtgt gtatctttta tgctttttaa
     3481 ttttgcctat tctgctttta acatctgtga gtgcttacgc tgtttctaat ccagttggag
     3541 agccctcaca tgcagttaag ccaaaaattg aaaagtatga tttatcacat tatcgttcaa
     3601 tatatagtga aaaaggcgac tggaatccgt ttggtcaaga agaaatttca cgacaaatta
     3661 ataatgtgtc agattttttc ttttctatga ctaagatttt agcaggtgtt acagattatg
     3721 cgattgaaaa tttatttcaa ctagatgtta ttaatgattt tgcggataaa atcggcgatt
     3781 ttgttggcga tatatatcaa aagttattga gtaatttggc aataacatta tttattattg
     3841 tttgctttaa cgcatttatt atttttagtg tacaaggtaa tgcaagagaa gcattaaaac
     3901 gggcgttttt aattatgtgt ttaattggtt ttggtgttgg tatacttgcg aatgcaggta
     3961 atattatacg tggtacaaac aatattggta aggacttaaa taatataatt atgaatagta
     4021 catcttctat tgatggtaat attgattata ttcatgagaa ttcaggtatg aatcatatac
     4081 gtaatcaatt atttgatatg acgatatata gaacatattt agtaatgaat tatggaactg
     4141 tagacgaaaa agagataaaa gcaaaaggta aagaccgtat tgataacatt ttaaaacttg
     4201 attatgataa aaaatcagaa aaagaattag atgatattgt taaagatgag gttgaaaaga
     4261 aaaataataa atatatgacg caaggttatg tatttcaaaa gttggctatt tcagtgattg
     4321 gctttattat tacacttttt atgtcgatag tatttttatc aataagtttt gcgaagttga
     4381 ttttttctac atttgcttta tttttattct tatttttagt attcagttgg atagtgagtt
     4441 ttataccagg atttgaatta tctgttttct ctgcgtttgc aaaaacgtta ggctatatta
     4501 ttttaagtgc ctgtatgaca ttcttatttg tgatagttgg tttgtgtatc aagcttgcaa
     4561 atagttttat tgatccggat agtcaaaatg cttatttttt aaattcaata tttataattg
     4621 taatattgtt tgtaatgtat aaaaaacggg ctcaaattat caattttgta agtcgtggta
     4681 atattagttt ttctcctagt gcaattggtg ctggtgtgat gaatcgaaca caagaacgtt
     4741 ttaataagat tcgtcatgaa caagcgcaaa ataagaaagc taaacgagaa aatcaaagag
     4801 atgaacccgc cccaccactt caaaatgata atgatttaag aagaagacag cagtataaac
     4861 caatgccttt atttataaat aaagaaaatc aaaaaaatgg taataagcga agagaacaac
     4921 aagagtcaat gaatggtaac gatgtaaaat ctgcttctgt tgaatcgaat gcaaataatt
     4981 attctaaaca accacaaaaa gcatcacagc aagaacatca agtacgagaa acaagacagc
     5041 gaaaagatat acaaaggtcg ccacaagtgg ttaatcagcc attaaataat gagaatcatt
     5101 caataaatcg taaagaacag aagtcagttc aaactgctta tgatactgat gttcaaaaac
     5161 gacaaataca aaatgcgaca cagaatcaac aaagtagaca atctggaaat cgtaatcaac
     5221 ctattacacg taattctcag tcaaaagatc gtctaaaaga gcaaaaggat atcaataagc
     5281 atggtaaata aaaaagatat tgctaaaaaa gtaataggta aaacgccaat aggtgttaaa
     5341 ttgaaattag cgaaagtcat catcattttg tgtgtggtgg ctttttttac atttccagta
     5401 attgtaatgt ttttgttagc gcctgattca aataaagata aagatgatgc tggttgtact
     5461 gttagtggcg gtaatgtttc ggcaaatggt attgataagt ttaatgaaaa tgctaaaggt
     5521 ggaaaattag agggtaaagg aaaagaaata cagaaaattg cagaaaaaaa taaagtacct
     5581 gtgaatattt ttatggctat tattgcgagt gaatcacaat ggggtaaagg agaaaatgcg
     5641 acaagacaaa ataatccttt gtctgttatg ggttcaaaat cgattcatga ttcaacatat
     5701 ccaactattg aagacggtct taatgcggga gctaaaaatt tgtatgatgt ttatatttct
     5761 aaaggtttag atactcctaa aaaaataggt cccaaatatg cgcctgttgg tgcgtcaaat
     5821 gaccctaata atatgaatgc ccgttggata ccaacagttg aaaaaattat gaaagattta
     5881 ggcggttcag aagctaaaac gtcatgtagt aatggtaaag gaaagtctat aaagtttaat
     5941 ggtaaattgc cacattggtc aaatgatgac cctggaaaag gtaatttata cactgctggt
     6001 caatgtactt ggtatgcata cggtatgcgt caaaaaatgg gtaaacctgt atcaacttat
     6061 tggcatgatg cacataaatg gaatgatagg gctaaggctg agggttataa agttgataaa
     6121 aatccggaac ctggtgcatt atttattgct gaacaaggtg caggcggtca tgatgggcat
     6181 tatggtcatg tggctgttgt tattggtgta agtgatggtg gtaaaacatt tagaataaca
     6241 gaaatgaatt gggaaggtgc ttttaaagtt aatgaacgta cattgaaaat gactgatgga
     6301 tatagtttta ttcatgataa ggagtgaaaa taaatgaagc aaaaaattat tatgtttact
     6361 cttgtaacag taatattatt ttgtgctgtt ttaattggtt atcagattcc taaacaacaa
     6421 gttaaaatga aacaaaatca aattgaagat ttacaggaag aacaacgtat attaagagat
     6481 aaaaatggag agttaaataa attagtaaag agacaatcaa aaacagttat ttctgatgaa
     6541 gaaaagcaaa tacgtgaagt ttcttcaaat tttgttaaac agatgtttga aatgaaaaaa
     6601 gattcttctt ttaaatcaaa agcaccacaa ataaagcctt tagttacaaa ggattattac
     6661 gatacattat ttaaggattc taaagataaa tatgatttat atgatgatat aacggttaat
     6721 gatattcatg tttattttga tacttatgat cctaaaaaag atagttataa agtttttgtt
     6781 cagttcgacg aacgtattga aacagatgga gatgataaaa ttgaacatcg tcaaacatct
     6841 gcacaattgg atttagttcg tacagctgaa ggttggcgta ttgataattt aaaacgtttc
     6901 aatttaaaac ctctaggacg ttagtttgtt tcaagacgat agcaatgata taatttacgt
     6961 aaaaaggagt gaatttatca tgaggcgatg gtttgtatta attttaggat tagttatatt
     7021 attatctgca tgtggtcaga aatatgataa agaaattgat gaagttacaa agttagaaaa
     7081 agaaagtatt caagatgtga aaaatacaaa gaagtataaa aatgttgagc ggtcaaaaag
     7141 ttattataag atttataatg atggtgaggt tattataatg acttatatgc cttttaaaga
     7201 tagtaataca aaagtttcaa gagtatataa aataaatcag actagcgatc aatatgaaga
     7261 agattctaat attgatcctg aaaagtttga gaaagataat aaaccagttt atgaagaaaa
     7321 taatatgaaa aaataaagaa tcaactccct atacgcttta gcggtggtat tgggagttga
     7381 tgaacatata tcctcttata acttatttta taaataacct ttttaaaagt aaaggaggat
     7441 atacatgaaa aaaagaaaaa aacgtaaaga attagactat aaaacacgtc tagctatcgc
     7501 aaagtattta aatgatggtt taaacatcat tcagatttct aagattttag gtcgtgatga
     7561 tgcggttctt tatagagaag taaataaacg ttctgttgat ggtgtttata atcctttatt
     7621 agctgaggaa gatgctataa aatcgcgtag tatttcagat gaacaagttg ctaaacgtag
     7681 acgtttgaat gatgaaacaa aagcatatat tgaagaaaaa ctttcattaa aatggtctcc
     7741 tagacagata gcaagtcaaa ttgaaaaaga tacagggcat tatataagtt atcctactat
     7801 atatagatat attcgcaatg gattggtaaa agttgatgtt aagcgtgata tgagacaagc
     7861 aggcaagaaa tataataagt cttctgaaaa gcgtggcaaa ttagacgtag ggtctcgtgt
     7921 tataaagtat cggctcaaaa agtattaata atcgtgaaga atttggacat tatgaaattg
     7981 atactgtttg gagtgttaga ccatcaacat attgcttgtt aactattatt gaaagaaaaa
     8041 caagatattt atatgctagt agattaaata cacgtaaatc caatgtagtt tgtaatgaga
     8101 ttataaatat tatgaaacca ttattaccca aa
//
LOCUS       DAMZPP010000125         8076 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMZPP010000125 DAMZPP010000000
VERSION     DAMZPP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.125"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     regulatory      86..188
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            268..957
                     /gene="tenA"
                     /locus_tag="POH73_002213"
     CDS             268..957
                     /gene="tenA"
                     /locus_tag="POH73_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HDI6888140.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLLGNIQADALRHYLQAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFESLMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            950..1780
                     /gene="thiD"
                     /locus_tag="POH73_002214"
     CDS             950..1780
                     /gene="thiD"
                     /locus_tag="POH73_002214"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDI6888141.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNNTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDADIAKDYLFTKEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            1764..2555
                     /gene="thiM"
                     /locus_tag="POH73_002215"
     CDS             1764..2555
                     /gene="thiM"
                     /locus_tag="POH73_002215"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDI6888142.1"
                     /translation="MNYLNNIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAQNEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVTIAKKAYAIYKTAIVIT
                     GKEDVIVQGDKAIVLANGSPLLARVTGAGCLLGGIIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            2557..3198
                     /locus_tag="POH73_002216"
     CDS             2557..3198
                     /locus_tag="POH73_002216"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDI6888143.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGFSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHTPVGPEMIATFKEM
                     NPQLPIVAIGGINTSNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            3284..4156
                     /gene="yidC"
                     /locus_tag="POH73_002217"
     CDS             3284..4156
                     /gene="yidC"
                     /locus_tag="POH73_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDI6888144.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(4313..4612)
                     /locus_tag="POH73_002218"
     CDS             complement(4313..4612)
                     /locus_tag="POH73_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001793952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888145.1"
                     /translation="MMWRCGVMGGIARGIDAMPLQFYDMSLGRCNVYYAVVTLLFHTV
                     TCICELLWKCCDIYCYTDESLCQDDIAMKNSNSVINHFLHGEISLILFQIKTAVG"
     gene            complement(4769..5416)
                     /locus_tag="POH73_002219"
     CDS             complement(4769..5416)
                     /locus_tag="POH73_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDI6888146.1"
                     /translation="MQQSDIISAAKKYMESIHQNDFTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEQMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQLLTEWNYHD"
     gene            complement(5444..6928)
                     /gene="cls"
                     /locus_tag="POH73_002220"
     CDS             complement(5444..6928)
                     /gene="cls"
                     /locus_tag="POH73_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDI6888147.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKVYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLHPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            7094..7387
                     /gene="csoR"
                     /locus_tag="POH73_002221"
     CDS             7094..7387
                     /gene="csoR"
                     /locus_tag="POH73_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HDI6888148.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            7399..7608
                     /locus_tag="POH73_002222"
     CDS             7399..7608
                     /locus_tag="POH73_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDI6888149.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVIINIIDGQVT
                     VAYETPANLNSIEKEIYDEGYKIVF"
     gene            7736..7873
                     /locus_tag="POH73_002223"
     CDS             7736..7873
                     /locus_tag="POH73_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888150.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
BASE COUNT         2840 a         1277 c         1467 g         2492 t
ORIGIN      
        1 atcatttctc tcccactccc aactgagcaa caataattga aaatgttgaa accgtttgct
       61 aagattaatt tgcaattgaa tactaaggaa ctactagggg agcctaatga tatggctgag
      121 atgaactgtt cagaccctta tgacctgatt tggttagtac caacgtagga aagtagttat
      181 tgaagtgatg gggctagtat agcaacattg ccatcaaact actttcatgt tggaagtagt
      241 ttttttattt agggaggatt ttttacaatg gaattttcac aaaaattgta ccaagctgca
      301 aagccaatca ttaatgatat ttatgaggat gactttattc aaaaaatgtt attaggcaat
      361 atacaagctg atgcattacg tcattattta caagctgatg cagcttattt aaaagaattt
      421 acaaacttat atgctttgtt aattccaaaa atgaatagca tgaatgacgt aaaattttta
      481 gtagagcaaa tagaatttat ggttgaaggt gaagtacttg cgcatgatat tttagcgcaa
      541 attgttggtg aatcatacga agaaatcatt aaaacaaaag tttggcctcc aagtggcgac
      601 cattacataa aacatatgta tttccaagcg catagtcgtg aaaatgctat ttatacaatt
      661 gctgcaatgg caccttgtcc atatatttat gcagaattag ctaagcgctc tcaaagtgat
      721 cataagctta atagagaaaa agatacggca aaatggtttg atttttatag tactgaaatg
      781 gatgacataa ttaacgtatt tgagtcatta atgaataaat tagccgaatc aatgtctgac
      841 aaggaattgg aacaagtaaa gcaagtattt ttagaaagtt gcatacatga gcgtcgattt
      901 ttcaatatgg ctatgacact agaacaatgg gaatttggag gaaaagtaaa tgattaaacc
      961 taaaatagca ttaaccattg caggtactga tccaacaggt ggtgccggcg taatggctga
     1021 tttaaaatca tttcattcat gtggtgtata tggtatgggc gtcgttacaa gtattgttgc
     1081 tcaaaataca ttgggcgtac aacatattca taatttaaat catcaatggg tagatgaaca
     1141 acttgatagt gtcttcaatg ataccttacc gcatgctatt aaaacgggga tgattgctac
     1201 agcagatact atggaaacga ttcgtcatta tttaatgcaa catgaatcta ttccgtatgt
     1261 aattgatcct gttatgttgg cgaaaagcgg tgattcatta atggataata acacaaagca
     1321 aaacttgcaa catacgttat tgccattagc tgatgtagta acaccgaatt taccagaagc
     1381 tgaagaaata acgggactaa ccattgatag tgaagaaaaa attatgcagg ctggccgcat
     1441 ctttattaat gagattggta gtaaaggtgt catcattaaa ggcggtcatt caaatgatgc
     1501 tgatatagca aaagattatt tatttactaa agaaggtgtt caaacatttg aaaatgaacg
     1561 atttaaaaca aaacatacgc atggaacagg gtgtacattt tcagcagtta taacggcaga
     1621 acttgcaaaa ggtagaccat tatttgaggc tgtacacaag gctaaaaagt ttatttcaat
     1681 gagtatacaa tatacgcctg aaatcggccg tggtagaggt ccagtgaatc attttgcata
     1741 tttaaagaaa gagggattag acgatgaatt atctaaataa catacgtatt gaaaacccat
     1801 taacaatttg ttatacaaac gatgtagtta aaaattttac agcgaatggt ttattaagta
     1861 ttggtgcgag ccctgcaatg agtgaagctc ccgaagaagc tgaagaattt tacaaagttg
     1921 cacaagcgct attaatcaat atcggtactt taacagccca aaatgaacaa gatattattg
     1981 cgattgctca aacggcaaat gaggcaggct tacctattgt atttgaccct gtagctgttg
     2041 gtgcttctac atatcgaaag caattttgta aattattatt gaaatcagcg aaagtatcag
     2101 taattaaagg caatgcatct gaaatattag cgttgattga tgatacagca actatgaaag
     2161 gtacagatag tgatgctaat cttgatgcgg ttacaatagc gaaaaaggct tacgcaatat
     2221 ataaaactgc aatagtaatc acaggtaaag aggacgttat tgttcaaggt gataaagcaa
     2281 tcgtattagc taatggatct ccattattag cacgagtaac tggagctggt tgtttattag
     2341 gaggcattat tgctggattt ttatttagag aaacagaacc agacatagaa gcgttaattg
     2401 aagcggtaag cgtatttaat attgctgctg aggtagctgc tgaaaatgaa aattgtggtg
     2461 gtcctggtac gttttcacca ttgttgcttg atacgttata tcatttaaat gaaacaacct
     2521 atcaacaacg gattcgcatt caagaggtgg aataatatgt ttaaccaatc gtatctaaat
     2581 gtgtatttta tttgtggaac ctccgatgtt ccgagtcatc gaactattca tgaagtatta
     2641 gaagcagcat taaaggcagg gattacactt ttccaatttc gagaaaaggg tgaatcagct
     2701 ttaaaaggaa acgataaact tgtattagct aaagaattac agcatctctg tcatcaatat
     2761 gatgtgcctt ttatcgttaa tgatgatgta tccttggcaa aagaaattaa cgcggatggt
     2821 atacatgtcg gtcaagatga tgcgaaagtc aaagagatag cacaatattt cactgataaa
     2881 attattggat ttagtattag tgatttagat gagtatgcaa aatctgattt aactcatgtt
     2941 gattatatag gtgttggacc aatctatcct acgccgtcga agcatgacgc gcatacacca
     3001 gtaggtccag aaatgattgc aacatttaaa gaaatgaatc cgcaactacc gattgtagcc
     3061 attggcggta taaatactag taacgtggcg ccgattgtag aagcaggtgc caatggaatt
     3121 tcagtcattt cagccatttc taaaagtgaa aatattgaaa agactgttaa tcgattcaaa
     3181 gattttttta ataattagtt aaatttttcc acttattgtt tcatacgtac tcattaatgt
     3241 gatagaataa atcctatgtg aatataatga tagaggtgga aaaatgaaga aaaaagcgtt
     3301 actaccatta tttttaggta ttatggtctt tttggctggt tgtgactatt ctaaacctga
     3361 aaaacgtagt gggtttttct acaatacatt cgtagatcca atgaaaaatg tattggattg
     3421 gttgggaaat aacttattaa acgacaatta tggtttagct attattattc ttgtattggt
     3481 aattcgtatt attttattac cattcatgtt gtcaaactat aaaaatagtc atatgatgcg
     3541 tcaaaaaatg aaagttgcaa agccagaagt tgagaaaatt caagaaaaag tgaagcgtgc
     3601 gcgtacacaa gaagaaaaaa tggctgcaaa ccaagaatta atgcaagtat ataaaaagta
     3661 tgacatgaac ccgattaaga gtatgttggg ttgtttacca atgctaatcc aattgccaat
     3721 catcatggga ttatactttg tacttaaaga ccaacttgta gatggtttgt ttaaatatcc
     3781 acacttctta tggttcgatt taggacgtcc tgatatttgg attacaatta ttgccggtgt
     3841 tttatacttt atccaagcat atgtatcaag taaaacgatg ccagacgaac aacgtcaaat
     3901 gggttacatg atgatggtca tttcaccaat tatgattatc tggatttcat taagctcagc
     3961 atcagcactt ggtttgtact ggtcagtcag cgcggcgttc cttgtagttc aaacacactt
     4021 tgcgaacatt tattatgaaa aagtcgctaa aaaagaagta cagcctttca ttgaagcgta
     4081 tgaaagagag cacaacggcg gcagcaataa aaaaggcaaa aacacacaag tagtgtctaa
     4141 aaagaaaaag aaataacctt agcaagtcca aataatgtgc cccctaaaag ttgaagataa
     4201 ttttaacttt atagggagca cattattttt cgcttcaata gcgaaactta gtgtcacatt
     4261 atacaatatg tgataaatag aaacagcact aagatgattt caaatgtgaa agtcatccta
     4321 ctgctgtttt tatttgaaat aatattaacg agatttcacc atgtaaaaag tggttaataa
     4381 cagagttgct attcttcatt gcaatgtcat cttgacacaa tgattcatca gtgtagcagt
     4441 atatgtcgca acatttccac agcaattcac aaatacatgt aacagtgtga aataataacg
     4501 tcacaacggc ataatacaca ttgcaacgtc ccaacgacat atcatagaat tgtaacggca
     4561 tggcatcaat accacgggct atgccaccca taacaccgca acgccacatc atagcactac
     4621 atcaagacgc atcgtggtac tagcacatca aagtagcatg gcatcaacac caagctccca
     4681 caacaacaca tcaaagtaac atggcatgac accaagcttc cacaacaaca cgctattaca
     4741 tcgcatcatc atactacaac tccaatgtct agtcgtgata attccattcc gttaaaagct
     4801 gtttcaagta catcatcata aagtcgtgac gttctttagc aatcatcttc gccgtatctg
     4861 tatgcattaa agattctaac ttaagtaatt tttcaaagaa atgtttaatt gcagatggtg
     4921 gcaactgttc aactaaatca tcattaatct tatctagtga catttgttct gtccacatag
     4981 gttcaccaaa gtgtcctgca aattgaaatg ttcgtgcaac acctatagcg cctatagcat
     5041 caagacgatc tgcatccctg acaatttgac cttctaaaga taaagtgaca tgatcgtttt
     5101 taccattgcg atagctcata ttattaataa taaataaaat gtgctcttga tcttcggttg
     5161 atagtgataa agaagataaa aatgacttca attcaacata ttgtttgtta gcatctacaa
     5221 ctttttcgtc aacggtatca tgaagtaaac atgcgagttc aatgactaaa gtatcattaa
     5281 caccttcatt ttcagcgatt gatttagcta aagcagtgac acgatataca tgcgcaatat
     5341 catggcctgt aaaatcattt tgatgaatag attccatata ttttttggct gcactaatga
     5401 tgtctgattg ttgcattaaa gttacactcc tcatatttct attttataag ataggtgaca
     5461 ataattgtga aataccttct ttaaatttaa tccaaagact tcgcttagca taacgtgctt
     5521 ttgttaattc agaagatact gctaaatcat ctataaaagc ttgttttaat tttttggcaa
     5581 tttgttggtc ataaataaag gcgttgactt cgaaattcaa cgtgaaactg cgatggtcca
     5641 tattagctgt tccaacactt gcaatttcat catctataac aagtgttttt gagtgtaaaa
     5701 agccattgtc gtagtgaaat actttaacac cggcatctaa taaggatgct gcatttttta
     5761 aagtagccca aaaaacaaac ggatggtcag gtttattagg aatcatgata ttgacatcaa
     5821 caccacctaa tgccgcaatt ttaatagaat ctaaaaaggc ttgatcaggt atgaaatagg
     5881 gagattgaat ataaatcgat tttttagcag atgaaatcat tttcaagtaa ccatatttaa
     5941 tctgttccca ttcttcgtca ggaccactag aagctatttg tacgccaatc gttccgccag
     6001 aatttacatc tgggaaataa cgatcatcat aggagatgtg gtcacgtgtg gcttgtgaat
     6061 tccaatctag aataaatcgt aattgcaatg cattcactgc atccccgaca attcgtaaat
     6121 gcgtatctcg ccaatagccg aatttttttg atttacctaa gtactcatca ccaacattaa
     6181 aaccaccaac atatccaatt tgtccatcta ttacaacaat ttttcgatgg tttcgattgt
     6241 tcatacgcaa gttaattaaa ggtaattttg atgggaaaaa tgcttcagca tgtccacctt
     6301 tattgcgaaa cgggtgtaag ccttttttac gcagtccacg agaacccatg tcatcataaa
     6361 gaattttaac ttctacacct tgttccgctt tttttccaag ttcatttaaa atggtacgac
     6421 ctaattcatc attttgaata atatagtact gaaaatgaat ataatcagta gcattacgga
     6481 tgtcttgtat taagtcatca aatttttctt ggccgtctgt gtatactttt aaatcgttgt
     6541 ctgttgttaa aaatgctgcg ttattatata acaacatttg aatcatttct ttaaatttta
     6601 caatttgata attggaattt gaaaagtttt cattttttaa agcggctaat tgctcatcaa
     6661 cgattaactc taatcctttt ttatcttcct tatcaatttt gaaaatttgg tcgcgttgaa
     6721 tttgtcgtcc taatagtaag tataaaatga agccgaataa aggcaagaaa actaagacta
     6781 gtaaccaagc ccagatagaa ttggcagaac gtctttccat gaaaataatg gtaaaggcga
     6841 ataataagtt taaaataaat gcaccaataa atatggaatt taaaataata ttagaatgct
     6901 tgagtgcaat ggataataac tctatcattt gaaacctccc atcgaaaatc ttaaacgagt
     6961 agtatatctt attaattgta tcatgaagca atttaaatat catgacaatt tagtttgaaa
     7021 aacacacttt gtgttgacgt tgtgttttat gggatatagt atagtaagga atgtaaatga
     7081 aggagtgaat gctatgactg aacaagataa tgcacatcat tctgaacaaa taaaaacgaa
     7141 tcttaaatca cgtttaaatc gaattgaagg acaagtgaga gcgattaatc gcatgattga
     7201 agaagatgtc tattgtgatg atgtccttac gcaaataaga gcgacacgtt cggcgttaaa
     7261 cagtgttgcg acaaagttat tagaacaaca tatgaaaagt tgtattatga ataaagttaa
     7321 tcaaggtgct caggaagagg caatggaaga gttattagtg acttttcaaa aattgattaa
     7381 agactaaagg agtttaagat gatacatcaa aatacgattt acacagcggg aattgaaaca
     7441 gaagaacaag taagtcaatt gacagaacgc atttcaaata tgataggtgt tcatcaagtg
     7501 attattaata taatagacgg tcaggtaact gtagcgtatg agacaccagc aaatttgaat
     7561 agtattgaaa aagaaatcta tgatgaagga tacaaaattg tattttaggg tataatgtag
     7621 agtgcgctat agattttaat tttgaaaata aattaaaaat tttgtaaatg atgtagtaaa
     7681 ggtatgtcga atagggtaag ataatattga aagcgatcca aacgaggtga taataatgaa
     7741 acgtcctgaa aagattcaaa atgtagtcaa actattgtca tcattaggtg tgaatattaa
     7801 aaaaactaaa tctcgtttag acattattaa tactttgcca gcatctaata aagtaagtca
     7861 cgaattaaaa taattgactg tgattgtgaa tcacacaccg ttaaagctat cgtcggacta
     7921 ttcgatgata gcttttttga ctggatttac taaaatgaaa gcaagtagta tataacaaaa
     7981 tccctctata gagaggctgg gacataaatc cctaaaaaaa cagcagtaag atatttttca
     8041 attaaaaaat atcttactgc tgttctctat ttatac
//
LOCUS       DAMZPP010000126         7988 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMZPP010000126 DAMZPP010000000
VERSION     DAMZPP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7988
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.126"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..225)
                     /locus_tag="POH73_002224"
     CDS             complement(<1..225)
                     /locus_tag="POH73_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HDI6888151.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITN"
     gene            390..1421
                     /locus_tag="POH73_002225"
     CDS             390..1421
                     /locus_tag="POH73_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDI6888152.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGD
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILKLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDSSGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            1914..3320
                     /locus_tag="POH73_002226"
     CDS             1914..3320
                     /locus_tag="POH73_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDI6888153.1"
                     /translation="MDVFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSIVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKPIPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVNKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHANQHLNQLTFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITKLMVIEKYEHVMHIVSEVTGEINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            3317..3883
                     /locus_tag="POH73_002227"
     CDS             3317..3883
                     /locus_tag="POH73_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDI6888154.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIIQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQHLMKIISSYQDKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDVPEQFSIMRYHSLISNPDNFPEELKITGRTDDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINYVKKG"
     gene            3889..4885
                     /gene="trpD"
                     /locus_tag="POH73_002228"
                     /pseudo
     CDS             3889..4885
                     /gene="trpD"
                     /locus_tag="POH73_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
     gene            4887..5669
                     /gene="trpC"
                     /locus_tag="POH73_002229"
     CDS             4887..5669
                     /gene="trpC"
                     /locus_tag="POH73_002229"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HDI6888155.1"
                     /translation="MTILEEIVEYKQLLLQNGYYQDKLNTLKSVNIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYEQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKPNHYYISESGIH
                     DVSDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            5669..6301
                     /locus_tag="POH73_002230"
     CDS             5669..6301
                     /locus_tag="POH73_002230"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HDI6888156.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPDHIDKVCVVVNPDLTTIEHVLSNTPINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKNIKDIPYLIAGG
                     INTENIQTVNQFKLSHQGFDLASGIEVNGRKDIEKMTAIVNIVKGDRENE"
     gene            6294..7508
                     /gene="trpB"
                     /locus_tag="POH73_002231"
     CDS             6294..7508
                     /gene="trpB"
                     /locus_tag="POH73_002231"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDI6888157.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEGGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGEDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEGGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMAERGLAND"
     gene            7501..>7988
                     /gene="trpA"
                     /locus_tag="POH73_002232"
     CDS             7501..>7988
                     /gene="trpA"
                     /locus_tag="POH73_002232"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDI6888158.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFEQLEKHGNQIKCQYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPFELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSYAEGFIYTVTM
                     NAT"
BASE COUNT         2934 a         1176 c         1514 g         2364 t
ORIGIN      
        1 atttgtgata gcaacaggtg ttgcataaat aattacatcg gcttttttaa tagcttcact
       61 ataatttaaa catttttcat taataatacc gattgattta gctttatcta actgagaagt
      121 atctgcatcg tatgcaataa tattagtatt agggttatgg tattttatat tgctagcaag
      181 acttccacca attaatccaa gcccaacaaa taaaactgtt gtcatataaa tcaccttatt
      241 tcgaaatttt cagaataata acattgtaaa tgagctgttg acacagtgca atagtaaata
      301 aaaatcgata atagcattaa tagataaacg gatataaatc atctacaata aagagtatag
      361 taacacaatg gcaacggagg ggtaaatcaa tggaaccaat attagaaatg attaaaacat
      421 taacaggtat taatagtcct tcaggagaca cagaagaagc aattcaattt gtcgaaaaat
      481 atgcaaaaga cttgggttat caaacaacac taacaaataa aggtgcgtta ttaataacag
      541 tgccaggcaa aaatgatgaa gtacaacgct gtattactgc tcatgttgat actttaggtg
      601 caatggttaa agaaattaaa gaagatgggc gcttagcaat agaattaatt ggaggattca
      661 cgtataacgc gattgagggt gaatattgcc aaattaaaac tgatgctggt caaatatata
      721 caggaaccat ttgtctgcat gaaacaagtg ttcatgtata tagaaataat catgaaatac
      781 ctagagacca aaagcatatg gaaataagaa ttgatgaagt aactacatca gaagaagata
      841 caaagagttt aggtatttca gtaggtgatt ttgttagctt tgatccacgt acagttatca
      901 cgtcatcagg ttttattaaa tctcgtcatt tagatgataa agctagcgta gcgatgatac
      961 taaaattact aaagaaatta aaagaagagc aaataatatt accacataca acgcaatttt
     1021 atatttctaa taacgaagaa ataggttacg gtgcaaatgc atcaattgat tcgaaaatca
     1081 aagaatatat tgcattagat atgggcgcgt tgggagacgg tcaagcatcc gatgaatata
     1141 cagtttctat ttgtgccaaa gattcttcag gtccatatca taagcaattg aaatcacacc
     1201 tagttaatct ttgcaaaata aataatattc catataaagt agacatatat ccatattatg
     1261 gttcagatgc ttcagcagct ttacatgctg gtgcggatat cagacatggt ttatttggcg
     1321 ctggcattga atcatctcat gcaatggaac gaacacatat tgattctatt aaagcgacag
     1381 agaaattact atatgcatat tgcttatcac caattgagta aacaattagt gttgacaaat
     1441 gtgaacgacc tatgtaatat aatgaactat aaaaataatt agaattttct aaagaaatag
     1501 tagcagatat gaaacgtagc aaatagaaag ctaatgggtg atgggaatta gcacgccata
     1561 tcttgtgaat tggactttgg aaaacaattg aatgagtttt gaaagtgaac atgaattatg
     1621 ttaactaagg tggcaccacg gtaacgcgtc cttacaggta tatgcgttat gtggtgtctt
     1681 tttatttaga caaaatgtag tagttagtta aaggtagcaa cagaaagtta gtgggtgatg
     1741 tgaactaaca ccgagattaa tgaaattggg ttttgtctgc aacagaaaaa ttatatatag
     1801 taaagagtga actatgaata tttcgaatat tcggttaatt taggtggtac cacgcgtcag
     1861 cgtcctttat attgataagg atgctgacgt ttttttagaa aggagcgtaa agaatggatg
     1921 tattctataa aaaaataaaa gcaaatgtaa cacctgaagt tttagcacaa cttcattcca
     1981 agaagatcat tttggaaagt acaaatcaac aacaaactaa gggtcgctat tctatagtga
     2041 tatttgatat ctacggtact ttaactttag ataacgatgt attatcagta agcactttaa
     2101 aagagtcgta tcaaatcact gaaagaccgt atcattattt aacgactaaa ataaatgaag
     2161 actaccataa tattcaagat gagcaactta agccaatacc gtttatatct ggatatgttg
     2221 ggacgtgtag ctttgattta gtaagacatg aatttcctaa attgcaatca atacaattag
     2281 aggatcacaa gcaacacgat gtacggttat atatggttga acaagtttat gtatttgacc
     2341 attacaaaga tgagttatat atcatcgcga cgaatcaatt ttcaaattca acgaaatcag
     2401 atcttgagaa tcgagttaat aagtctatcg aagacttaac taaaatccaa ccattcatgc
     2461 ctacacaaga ttttgatttt aaaactaaag aaattcaatc aaatatttct gaagaaagat
     2521 ttatcgaaat gattcagtat ttcaaagaga aaataacaga aggggatatg ttccaagttg
     2581 tgccatcaag aatttacaaa tatgcgcatc atgctaatca gcatttaaat caacttacgt
     2641 ttcaactgta tcaaaattta aaacgacaaa acccaagtcc atatatgtat tatcttaata
     2701 tcgatcaacc ctacattgtc ggtagttctc ccgaaagttt cgtaagtgtc aaagatcaaa
     2761 ttgtaacaac taatcctatt gcaggtacga ttcaacgtgg tgagacgaca caaatagata
     2821 atgagaatat gaaacaacta cttaatgatc ctaaagaatg cagtgaacat cgtatgcttg
     2881 ttgatttagg gcgtaatgat attcatagag taagtaaaat aggtacctca aaaattacta
     2941 aattaatggt tattgaaaaa tatgaacatg ttatgcatat cgtaagtgaa gtcacaggtg
     3001 aaataaatca aaatttatcg ccaatgacag ttattgcgaa tttattacca acaggtaccg
     3061 tttcaggtgc accaaaatta cgtgcaatcg aaagaattta cgaacaatat ccacataaac
     3121 gtggcgttta tagtggtggt gttggataca taaattgtaa tcataactta gattttgcat
     3181 tagcaattcg aacgatgatg atagatgagc agtatatcaa cgtagaagct ggttgtgggg
     3241 ttgtatatga ttctattcct gaaaaagaac taaatgaaac gaaattgaaa gccaaaagtt
     3301 tattggaggt aagcccatga ttttagttgt agataattat gattccttta cgtataacct
     3361 agtggatatt gtggcacaac acactgatgt cattattcaa taccctgatg atgataatgt
     3421 gctgaatcaa tctgttgatg ctgtcattat ttcaccaggc cctgggcatc cattagacga
     3481 ccaacattta atgaaaatta tctcatccta tcaagataaa cccattttag gtatttgcct
     3541 aggtgctcaa gcactgactt gttattacgg tggagaagtc attaaaggcg acaaagttat
     3601 gcacggaaaa gttgatacgc ttaaggttat atcgcatcat caacatctgt tatatcaaga
     3661 tgtaccagaa caattttcta ttatgagata ccattcatta ataagtaatc ctgataattt
     3721 tccagaagaa ttgaaaatta ctggacgtac tgatgattgt atacaatcat tcgagcataa
     3781 agaaagaccg cattatggta ttcagtacca tcctgaatca tttgctacag actatggtgt
     3841 caaaataatt acaaatttta ttaattacgt gaagaaagga tgaaaaccat gacattacta
     3901 acaagaatcc aaactgaatc tatattgctt gaaagtgaca ttaaggagtt aatcgacata
     3961 cttatttccc caagtatcgg aactgatata aaatatgaat tactgagttc gtattcggaa
     4021 cgagaaattc aacaacaaga attgacatat attgtacgta gtttaattaa tacaatgtac
     4081 ccacatcagc cttgttatga gggcggtatg tgtgtgtggt acaggtggtg acaagtctaa
     4141 tagcttcaat atatcaacta ctgtcgcttt tgttgtagca agtgctggcg taaaagttat
     4201 aaaacatgga aataaaagtg tgacttcaaa ttcaggtagt acggatttgt taaatcaaat
     4261 ggacattcaa actacgtctg ttgatgatac gccaaagcaa ttagatgaaa aaaacctcgt
     4321 gtttatcggc gcaacagaat cgtatccaat aatgaaatat atgcaaccag ttagaaaaat
     4381 gataggtaag cctacaatat taaaccttgt aggtccatta attaatccct atcacctaac
     4441 gtatcaaatg gtaggtatct ttgaccctac aaaactaaaa ctagttgcaa agacaattaa
     4501 agatttaggt agacgacgtg caatcgtatt acatggtgcg aatggtatgg atgaagcaac
     4561 gttgtctggt gataatttga tatatgaatt gactgaagat ggagaaatca aaaattacac
     4621 attaaatgcg actgattatg gtttgaaata tgcacagaat agtgatttta aaggtggttc
     4681 gcctgaagaa aacttagcaa tcacacttaa tatcttgaat ggtaaagatc agtcaagtcg
     4741 ccgtgatgtt gtcatactta atgcaggttt aagtctatat gttgcagaga aagtggatag
     4801 catcgcagaa ggcatagaac tcgcaagcac tttgatagat aatggtgaag ctttgaaaaa
     4861 ataccatcaa atgagaggtg aataacatga caattttaga agaaattgtt gaatataaac
     4921 agttactttt acaaaatggc tattatcaag acaaacttaa taccttgaaa agtgtgaaca
     4981 ttcagaataa aaaatctttt ataaatgcaa ttgagaaaga accaaagcta gcaattattg
     5041 cagaaattaa atcgaagagt cctacagtta atgacttacc tgaacgagat ttatcgcaac
     5101 aaatctcaga ttatgagcaa tatggtgcaa atgctgtatc cattttgact gatgaaaagt
     5161 actttggtgg tagttttgaa agattacaag cattgacaac aaaaacaaca ttacccgtat
     5221 tatgcaaaga ttttattata gatccgcttc aaattgatgt tgccaaacaa gctggtgcat
     5281 ctatgatttt actgattgtt aatatcttat cggataaaca attgaaagat ttatataact
     5341 acgctatatc gcaaaattta gaagtgttag ttgaagtaca tgatcgtcat gaattagaac
     5401 gtgcctataa ggttaatgct aaattgattg gtgtaaataa cagggactta aaacgatttg
     5461 tcacaaatgt ggaacataca aatactattt tagaaaataa aaaaccaaat cattattata
     5521 tttctgaaag tggaattcac gatgtatctg atgtaagaaa aatcttgcat agtggtatcg
     5581 atggcttact aataggtgag gcgcttatgc gttgtgacaa tctatctgaa tttttaccac
     5641 aactgaaaat gcaaaaggtg aagtcatgat gaaattgaaa ttttgtggct ttacatcaat
     5701 taaggatgtt acagcggcca gtcaattacc tattgatgcg ataggtttca tccattatga
     5761 aaaaagtaaa aggcatcaaa caattaccca aataaaaaag ttagcgtctg ctgttccaga
     5821 tcatattgac aaagtatgtg tcgtggtaaa tcctgattta acaactattg aacacgtatt
     5881 aagcaatacg ccaattaaca caatacagtt acacggcaca gaatctattg attttataca
     5941 ggaaataaaa aagaagtatt caagcattaa aattactaaa gctttagctg cagatgaaaa
     6001 cataatccaa aacataaata aatataaagg gttcgtagat ttatttatta tcgacacccc
     6061 ctcagtgtcg tatggtggta ccggtcaaac atatgactgg actattttga agaacataaa
     6121 agacatacct tacttgatag caggaggcat taacactgaa aatattcaaa cagttaatca
     6181 atttaaatta tcacatcaag gttttgatct tgcatcaggt atagaagtaa atgggcgaaa
     6241 agatatagaa aaaatgacag caattgtaaa tattgtgaaa ggagatagag aaaatgaata
     6301 aacaaataca aacagaagca gatgaattag gtttctttgg tgaatacgga gggcaatatg
     6361 ttccagaaac attaatgcca gcaattattg agttgaaaaa agcttataaa gaggcaaaag
     6421 cagacccaga gtttcaaaga gaacttgaat actatttatc agagtatgta ggacgcgcga
     6481 cgccacttac atatgctgca tcgtatactg aaagcttagg tggcgctaag atatatttaa
     6541 aacgagagga tctaaatcat acaggcgccc ataaaattaa taatgcgtta ggtcaagcgt
     6601 tgctcgctaa aagaatgggc aagaagaagc ttgttgctga aacaggtgcg ggtcaacatg
     6661 gtgtagctag tgctacggtt gctgcattat ttgatatgga acttgttgtc tttatgggaa
     6721 gtgaagatat taaaagacaa caacttaatg tatttagaat ggaattactt ggtgcaaagg
     6781 ttgtggcagt tgaaggtggt caagggactt tatcggatgc agttaataaa gcattgcaat
     6841 attgggtaag tcatgtagat gatacgcatt atttattagg ttctgcattg ggtccagacc
     6901 cattcccaac gattgttaga gattttcaga gtgtgattgg taaagaaata aaatcacaga
     6961 tattgaagaa agaaggtcga cttccggatg caattgtagc atgtatcggt ggtggctcaa
     7021 atgcaatcgg tacattttat ccatttatta aagatgatgt tgctttatac ggtgttgaag
     7081 ccgcaggtca aggcgaggat actgataaac atgcacttgc aattggcaaa ggatcacctg
     7141 gcgtattaca tggtactaaa atgtatttaa ttcaagatga aggtgggcaa gtgcaactag
     7201 cacattctat ttcagcagga cttgattatc ctggtattgg gccagaacat tcttattacc
     7261 acgacattgg tagagtaact tttgaaaatg caagtgatac acaagcaatg aatgctttaa
     7321 tcaactttac aaaacatgaa ggtattatac ctgcaattga aagtgcacat gcactgagtt
     7381 atgttgaaag actagcgcct acgatgtcga aagaagatat tattgtagta acaatttctg
     7441 gacgtggcga taaagatatg gaaacaatta gacaatatat ggcagagcga ggtcttgcaa
     7501 atgactaaat tatttatacc ttatattatg ggcaataaag atttgattga aaatgcaaca
     7561 ttgttgagtg aaaatggtgc agatataatt gaaattggtg tacctttctc tgatccggtt
     7621 gctgatggtc cagttatcat ggaagcaggt caacaagcga ttaaacaagg cataacgata
     7681 gattatattt tcgagcaatt agaaaaacac ggtaatcaaa ttaagtgtca gtatgtatta
     7741 atgacgtatt ataatataat ttgtcattat ggagaacaag cgttttttga aaaatgtcga
     7801 gataccggtg tctacggctt aattattcct gatttaccat ttgaattatc gcagcgttta
     7861 aaacaacaat ttagtcacta tggtgtcaaa atcatatcgt tagttgcgat gactactgat
     7921 gacaaacgta taaaagatat cgtatcatat gcggaaggct ttatttatac tgtgacaatg
     7981 aatgcgac
//
LOCUS       DAMZPP010000127         7951 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMZPP010000127 DAMZPP010000000
VERSION     DAMZPP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.127"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>7951
                     /locus_tag="POH73_002233"
     CDS             <1..>7951
                     /locus_tag="POH73_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GA module-containing protein"
                     /protein_id="HDI6888159.1"
                     /translation="YNNAVTTAQGIIDQTTSPTLDPTVINQAAGQVSTTKNALNGNEN
                     LEAAKQQATQSLGSLDNLNNAQKQAVTDQINGAHTVDEANQIKQNAQNLNTAMGNLKQ
                     AIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNATQTEVEQAIQQVNA
                     AKQALNGNANVQHAKDEATALINSSNDLNQAQKDALKQQVQNATTVAGVNNVKQTAQE
                     LNNAMTQLKQGIADKEQTKADGNFVNADPDKQNAYKQAVAKAEALISGTPDVVVTPSE
                     ITAALNKVTQAKNDLNGNTNLATTKQNVQHAIDQLPNLNQAQRDEYSKQITQATLVPN
                     VNAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTAYDNAVTKAEELLKQ
                     TTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQAAKTNLGTLDHLNDAQKQALTT
                     QVEQAPDIATVNNVKQNAQNLNNAMTNLSNALQDKTETLNSINFTDADQAKKDAYTNA
                     VAHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRAKDAAKQVITNANDL
                     NQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIADKDQTKANGNFVNAD
                     TDKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVTQAKGDLNGNHNLQVAKDNA
                     NTAIDQLPNLNQTQKTALKDQVSHAELVTGVNAIKQNADALNNAMGTLKQQIQANSQV
                     PQSVDFTQADQDKQQAYNNAANQAQQIANGTPTPVLTPDAVTQAVTTMNQAKDALNGD
                     EKLAQAKQDAIANLDTLRDLNQPQRDALRNQINQAQALATVEQTKQNAQNVNTAMSNL
                     KQGIANKDTVKASENYHDADADKQTAYTNAVSQAEGIINQTTNPMLNPDDITRALTQV
                     TDAKNGLNGEAKLATEKQNAKDAVNAMTHLNDAQKQALKGQIDQSPEIAIVNQVKQTA
                     TSLDHAMDQLSQAINDKAQTLADGNYLNADPDKQNAYKQAVAKAEALLNKQSGTNEVQ
                     AQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQKQSFESQITQAPLV
                     TDVTTINQKAQALDHAMELLRNSVADNQATLASEDYHDATAQRQNDYNQAVTATNNII
                     NQTTSPTMNPDEVNRATTQVNNTKVALDGDENLVAAKQQANNRLDQLDHLNNAQKQQL
                     QSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQTVEQLGNFVNADTDKQTAYT
                     TAVNEAEAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQVAKANATQAIDALT
                     SLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRESIQDNAATKANSKYIN
                     EDQSEQQNYDQAVQAANNIINEQTATLDNNAINQAATTVNTTKAALHGDVKLQNDKDH
                     AKQTVSQLTHLNNAQKHMEDTLIDSETTRTAVNHDLTEAQALDQLMDALQQSIADKDA
                     TRASSAYVNAEPNKKQSYDEAVQNAESIIAGLNNPTINKGNVTSATQAVTSSKNALDG
                     VERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRDKVAEIIAQAQALNEAMKA
                     LKESIKDQPQTEASSKFINEDQAQKDAYTQAVQHAKDLINKTTDPTLAKSIIDQATQA
                     VTDAKNNLHGDQKLDQDKQRATETLNNLSNLNTPQRQALENQINNAATRGEVAQKLTE
                     AEALNQAMEALRNSIQDQQQTEAGSKFINEDKPQKDAYQAAVQNAKDLINQTNNPTLD
                     KAQVEQLTQAVNQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNPQRQALESQINNAAT
                     RDEVAQKLAEAKALDQAMQALRNSIQDQQQTESGSKFINEDKPQKDAYQAAVQNAKDL
                     INQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQLQAVTTVNALPNLNHAQQQA
                     LTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQPNYTESSTDKKEAV
                     DQALQAAESITDPTNGSNANKDAVDQALTKLQEKVNELNGNERVAEAKTQAKQTIDQL
                     THLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQAVNEHANVEQTVDYT
                     QADLDKQNAYKQAIADAENVLKQNANKQQVDQALQNILNAKQALNGDERVALAKTNGK
                     HDIEQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNRVMDQLSQGITGNEGRT
                     KGSTNYVNADTQVKQVYDEAVDKAKQALDKSTGQNLTAEQVIKLNDAVTAAKKALNGE
                     EKLNNRKSEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRAINRATKLDNAMGAV
                     QQYIDEQHLGVISSTNYINADDNLKANYDNAIANAAHELDKVQGNAIAKAEAEQLKQN
                     IIDAQNALNGDQNLANAKDKANAFVNSLNGLNQQQQHLAHNAINNADTVSDVTDIVNN
                     QIDLNDAMETLKHLVDNEIPNAEQTVNYQNAEDNAKTNFDDAKRLANTLLNSDNTNVN
                     DINGAIQTVNDAIQNLNGDQRLQDAKDKAIQSINQALANKLKEIEASNATDQDKLIAK
                     NKAEELANSIINNINKATSNQDVSQVQTAGNHAIEQVHANEIPKAKIDANKDV"
BASE COUNT         3387 a         1463 c         1376 g         1725 t
ORIGIN      
        1 cgtacaataa tgctgtcaca actgcacaag gtattattga tcaaacgaca agtccaactt
       61 tagatccgac tgttatcaat caagctgctg gacaagtaag cacaactaaa aatgcattaa
      121 atggtaatga aaacctagag gcagcgaaac aacaagcgac acaatcatta ggttcattag
      181 acaacttaaa taatgcgcaa aaacaagcag ttactgatca aattaatggc gcgcatactg
      241 ttgatgaagc aaatcaaatt aaacaaaatg cgcaaaactt aaatacagcg atgggtaact
      301 tgaaacaagc gattgctgac aaagatgcta cgaaagcaac agtaaacttc actgatgcag
      361 atcaagcaaa acaacaagca tataacactg ctgttacaaa tgctgaaaac atcatttcaa
      421 aagctaatgg tggcaatgca acacaaactg aagttgaaca agcaatccaa caagttaatg
      481 ctgcaaaaca agcattaaat ggtaatgcca acgttcaaca tgcgaaagac gaagcaacag
      541 cattaattaa tagctctaat gaccttaacc aagcacaaaa agacgcatta aaacagcaag
      601 ttcaaaatgc aactactgta gctggtgtaa acaatgttaa acaaacagcg caagagttaa
      661 acaatgcaat gacacaatta aaacaaggca ttgcagataa agaacaaaca aaagctgatg
      721 gtaactttgt caatgcagat cctgacaaac aaaatgcata taaacaagca gtagcaaaag
      781 ctgaagcatt aattagcggc acacctgatg ttgtcgttac acctagcgaa attactgcag
      841 cgttaaataa agttacacaa gctaaaaatg atttaaatgg taatacgaac ttagcaacaa
      901 cgaaacaaaa tgttcaacat gctattgatc aattgccaaa cttgaatcaa gcgcaacgtg
      961 atgaatacag caaacaaatt acgcaagcaa cacttgtacc aaacgtcaat gctattcaac
     1021 aagcggcaac tacgcttaat gatgcgatga cacaattgaa acaaggtatt gcgaataaag
     1081 cacaaattaa aggtagcgag aactatcacg atgctgatac tgacaagcaa acagcatatg
     1141 ataatgcagt aacaaaagca gaagaattgt taaaacaaac aacaaatcca acaatggatc
     1201 caaatacaat tcaacaagca ttaactaaag tgaatgatac aaatcaagca cttaacggta
     1261 atcaaaaatt agctgatgct aaacaagctg ctaagacaaa tcttggcaca ttagatcatt
     1321 taaatgatgc gcaaaaacaa gcgttaacaa cacaagttga acaagcacca gatattgcaa
     1381 cagttaataa tgttaagcaa aatgctcaaa atctgaataa tgctatgact aacttaagta
     1441 atgcattaca agataaaact gagacattaa atagcattaa ctttactgat gcagatcaag
     1501 ctaagaaaga tgcttatact aatgcggttg cacatgcaga aggtatttta tctaaagcaa
     1561 atggcagcaa tgcaagtcaa accgaagtgg aacaagcgat gcaacgcgtg aacgaagcga
     1621 aacaagcatt gaatggtaat gacaatgtac aacgtgcaaa agatgcagcg aaacaagtga
     1681 ttacaaatgc gaatgattta aatcaagcgc aaaaagatgc gttaaaacaa caagtcgatg
     1741 ctgcgcaaac tgttgcaaat gtaaacacga ttaagcaaac agcacaagat ttaaatcaag
     1801 caatgacaca attgaaacaa ggtattgcag ataaagacca aactaaagca aatggtaact
     1861 ttgtcaatgc tgatactgat aagcaaaatg catacaacaa tgcggtagca catgctgaac
     1921 aaatcattag tggtacacca aatgcaaacg tggatccaca acaagtggct caagcgttac
     1981 aacaagtgac tcaagctaag ggtgatttaa acggtaacca taacttacaa gttgctaaag
     2041 acaatgcgaa tactgccatt gatcagttac caaacttaaa tcaaacacaa aaaacagcat
     2101 taaaagacca agtgtcacat gcagaacttg ttacaggtgt taatgctatt aagcaaaatg
     2161 ctgatgcatt aaataatgca atgggtacgt tgaaacaaca aattcaagca aacagtcaag
     2221 taccacaatc agttgacttt acacaagcgg atcaagacaa acaacaagca tataacaatg
     2281 cagctaacca agcgcaacaa attgcaaatg gcacaccaac acctgtattg acgcctgacg
     2341 cagtaacaca agcagttact acaatgaatc aagcgaaaga tgcattaaac ggtgatgaaa
     2401 aattagcgca agcgaaacaa gatgcaatag caaatctaga tacgttgcgt gacttaaatc
     2461 aaccacaacg tgatgcgtta cgtaatcaaa tcaatcaagc acaagcgtta gctacagttg
     2521 aacaaactaa acaaaatgca caaaatgtta atacagcaat gagtaacttg aaacaaggta
     2581 ttgcaaataa agataccgtc aaagcaagtg aaaactatca cgatgctgat gccgataagc
     2641 aaacagcata tacaaatgca gtgtctcaag cggaaggtat tatcaatcaa acgacaaatc
     2701 caatgcttaa cccagatgat attactcgtg cattaactca agtgactgat gctaaaaatg
     2761 gcttaaacgg tgaagctaaa ttggcaactg aaaagcaaaa tgctaaagat gctgtcaatg
     2821 caatgacgca tttgaacgat gctcaaaaac aagcattaaa aggacaaatc gatcaatcgc
     2881 ctgaaattgc tatagtgaac caagttaaac aaacagcaac gagcctagat catgcaatgg
     2941 atcaattatc acaagctatt aatgataaag ctcaaacatt agcggacggt aattacttaa
     3001 atgcagatcc tgacaaacaa aatgcgtata aacaggcagt agcaaaagct gaagcattat
     3061 tgaataaaca aagtggtact aatgaagtac aagcacaagt tgaaagcatc actaatgaag
     3121 tgaacgcagc gaaacaagca ttaaatggta atgataattt agcgaatgct aagcaacaag
     3181 cgaaacaaca actggcgaat ctaacacact taaatgatgc acaaaaacaa tcatttgaaa
     3241 gtcaaattac acaagcgcca cttgttacag atgtcactac aattaatcaa aaagcacaag
     3301 cgttagatca tgcgatggaa ttattaagaa atagtgttgc ggataatcaa gcgacattag
     3361 cgtctgaaga ttatcatgat gcaactgcgc aaagacaaaa tgactataac caagcagtaa
     3421 cagctactaa taatatcatt aaccaaacaa catcacctac aatgaatcca gatgaagtta
     3481 atcgtgcaac gacacaagtg aataacacga aagttgcatt agatggtgat gaaaaccttg
     3541 tagcagctaa gcaacaagcg aacaatagac ttgatcaatt agatcattta aataatgcgc
     3601 aaaagcaaca gttacaatca caaattacgc aatcatctga tattgctgca gttaatggtc
     3661 acaaacaaac agcagaatct ttaaatacag caatgggtaa cttaattaat gcgattgcag
     3721 atcatcaaac cgttgaacaa cttggtaact tcgtcaatgc tgatactgat aaacaaactg
     3781 cttatactac agcggtaaat gaagcagaag ccatgattaa caaacaaact ggtcaaaatg
     3841 cgaaccaaac agaagtagaa caagctatta ctaaagttca aacaacactt caagcgttaa
     3901 atggagatca taatttacaa gttgctaaag caaatgcgac gcaagcaatt gatgctttaa
     3961 caagcttaaa tgatcctcaa aaaacagcat taaaagacca agttactgct gcaactttag
     4021 taactgcagt tcatcaaatt gaacaaaatg cgaatacgct taaccaagca atgcatggct
     4081 taagagaaag tattcaagat aacgcagcaa ctaaagcaaa tagcaaatat atcaacgaag
     4141 atcaatcaga acaacaaaac tacgatcaag cagtgcaagc cgcaaataat attatcaatg
     4201 aacaaacagc aacattagat aataatgcaa ttaatcaagc agcgacaact gtgaatacaa
     4261 cgaaagcagc attacatggt gatgtgaaat tacaaaatga taaagatcat gcgaaacaaa
     4321 cagttagtca attaacacat ttaaacaatg ctcaaaaaca tatggaagat acgttgattg
     4381 atagtgaaac aactagaaca gccgttaacc atgatttgac tgaagcacaa gcattagatc
     4441 aacttatgga tgcattacaa caaagtattg ctgacaaaga tgcaacacgt gcgagcagtg
     4501 catatgtcaa tgcagaaccg aataaaaaac aatcatatga tgaagcagtt caaaatgctg
     4561 agtctatcat tgcgggatta aataatccga ctatcaataa aggtaatgta acaagtgcga
     4621 cacaagctgt aacatcatct aaaaatgcat tagacggtgt tgaacgatta gcgcaagata
     4681 agcaaactgc tggaaattct ctaaatcatt tagaccaatt aacaccagca caacaacaag
     4741 cactagaaaa tcaaattaat aatgcaacaa ctcgtgataa agtggctgaa attattgcac
     4801 aagcgcaagc attaaatgaa gcgatgaaag cattaaaaga aagtattaaa gatcaaccac
     4861 aaactgaagc aagtagtaaa tttattaatg aagatcaagc acaaaaagat gcttatacgc
     4921 aagctgtaca acacgcgaaa gatttgatta acaaaacaac tgatcctaca ttagctaaat
     4981 caatcattga tcaagcgaca caggcagtga ctgatgctaa aaacaattta catggtgatc
     5041 aaaaactaga tcaagataag caacgtgcaa cagaaacgtt aaataacttg tctaacttga
     5101 atacaccaca acgtcaagca cttgaaaatc aaatcaataa tgcagcaact cgtggcgaag
     5161 tagcacaaaa attaactgaa gcagaagcac ttaaccaagc aatggaagct ttacgtaata
     5221 gcattcaaga tcaacagcaa acggaagcgg gtagcaagtt tatcaatgaa gataaaccac
     5281 aaaaagatgc ttaccaagca gcagttcaaa atgcaaaaga tttaattaat caaactaaca
     5341 atccaacgct tgataaagca caagttgaac aattgacaca agctgttaac caagctaaag
     5401 ataacctaca cggtgatcaa aaacttgcag acgataaaca acatgcggtt actgatttaa
     5461 atcaattaaa tggtttgaat aatccgcaac gtcaagcact tgaaagccaa ataaacaacg
     5521 cagcaactcg tgatgaagta gcgcaaaaat tagctgaagc aaaagcgctt gatcaagcaa
     5581 tgcaagcatt acgtaatagt attcaagatc aacaacaaac agaatctggt agcaagttta
     5641 tcaatgaaga taaaccgcaa aaagatgctt accaagcagc agttcaaaat gcaaaagatt
     5701 taattaacca aacaggtaat ccaacactcg acaaatcaca agtagaacaa ttgacacaag
     5761 cagtaacaac tgcaaaagat aacctacatg gtgatcaaaa acttgctcgt gatcaactac
     5821 aagcagtaac aactgtaaat gcattgccaa acttaaatca tgcacaacaa caagcattaa
     5881 ctgatgctat aaatgcagcg cctacaagaa cagaggttgc acaacatgtt caaactgcta
     5941 ctgaacttga tcacgcgatg gaaacattga aaaataaagt tgatcaagtg aatacagata
     6001 aggctcaacc aaattacact gaatcgtcaa ctgataaaaa agaagcagta gatcaagcgt
     6061 tacaagctgc agaaagcatt acagatccaa ctaatggttc aaatgcgaat aaagacgctg
     6121 tagaccaagc attaactaag cttcaagaaa aagtgaatga gttaaatggt aatgagagag
     6181 tcgctgaagc taaaacacaa gcgaaacaaa ctattgacca attaacacat ttaaatgctg
     6241 atcaaattgc aactgctaaa caaaatattg atcaagcgac gaaacttcaa ccaatcgctg
     6301 aattagtaga tcaagcaacg caattgaacc aatcaatgga tcaattacaa caagcagtta
     6361 atgaacatgc taacgttgag caaactgtag attacacaca agcagattta gataagcaaa
     6421 atgcttataa acaagctatt gctgatgccg aaaatgtatt gaaacaaaat gcgaataagc
     6481 aacaagtgga tcaagcactt caaaatattt taaatgcaaa acaagcatta aatggtgatg
     6541 aacgtgtagc acttgctaaa acaaatggta aacatgatat agagcaattg aatgcattaa
     6601 acaatgctca acaagatgga tttaaaggtc gcatcgatca atcaaacgat ttaaatcaaa
     6661 tccaacaaat tgtagatgag gctaaggcac ttaatcgtgt aatggatcaa ttgtcacaag
     6721 gaatcactgg caatgaagga cgcacgaaag gtagcacgaa ctatgtcaat gcagatacac
     6781 aagttaaaca agtgtatgat gaagcggttg ataaagcgaa acaagcactt gataaatcga
     6841 caggtcaaaa cttaactgca gaacaagtta tcaaattaaa tgatgcagtc actgcagcaa
     6901 agaaagcatt aaatggtgaa gaaaaactta ataatcgtaa gtctgaagca ttacaaagat
     6961 tggatcaatt aacacatcta aacaatgctc aaagacaatt agcaatccaa caaattaata
     7021 atgctgaaac gctaaataaa gcatctcgag caattaatag agcaactaaa ttagataatg
     7081 caatgggtgc agtacaacaa tatattgacg aacagcacct tggtgttatc agcagcacaa
     7141 attacatcaa tgcagatgat aatttgaaag caaattatga taatgcaatt gcgaatgcag
     7201 cacatgagtt agataaagtg caaggtaatg caattgcaaa agctgaagca gagcaattga
     7261 aacaaaatat tattgatgct caaaatgcat taaatggaga ccaaaacctt gcaaatgcca
     7321 aagataaagc aaatgcgttt gttaattcgt taaatggatt aaatcaacag caacaacatc
     7381 ttgcacataa tgcaattaac aatgccgata ctgtatcaga tgtaacagat attgttaata
     7441 atcaaattga cttaaatgat gcaatggaaa cattgaaaca tttagttgac aatgaaattc
     7501 caaatgcaga gcaaactgtc aattaccaaa acgctgaaga taatgctaaa acaaacttcg
     7561 atgatgctaa acgtctagca aatacattgc taaatagtga taacacaaat gtgaatgata
     7621 ttaacggcgc aattcaaaca gtcaatgatg caatccaaaa tcttaatggt gatcaacgac
     7681 tacaagatgc taaagacaag gcaattcaat ctattaatca agctttagct aataagctaa
     7741 aagaaatcga agcttcaaat gcgacggatc aagacaagct tattgcgaaa aataaagcag
     7801 aagaattggc aaacagcatc atcaacaaca ttaataaagc aacaagtaat caggatgtat
     7861 ctcaagttca aacagctggc aaccatgcga ttgaacaagt gcatgccaat gaaataccga
     7921 aagctaaaat tgatgcgaat aaagatgttg a
//
LOCUS       DAMZPP010000128         7856 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMZPP010000128 DAMZPP010000000
VERSION     DAMZPP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7856
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.128"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(108..734)
                     /locus_tag="POH73_002234"
     CDS             complement(108..734)
                     /locus_tag="POH73_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDI6888160.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYENQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            931..2145
                     /locus_tag="POH73_002235"
     CDS             931..2145
                     /locus_tag="POH73_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888161.1"
                     /translation="MSYHWFKKMLLSTSMLILSSSSSLGLATHTVEAKDNLNGEKPTT
                     NLNHNVTSPSVNSEMNNNETGTPHESNQAGNEGTGSNSRDANPDSNNVKPDSNNQNPS
                     PDSKPDPNNPNPGPNPKPDPDKPKPNPEPKPDPDKPKPNPDPKPDPDKPKPNPDPKPD
                     PNPNPNPKPDPNKPNPNPSPNPNQPGDSNQSGGSKNGGTWNPNASDGSNQGQWQPNGN
                     QGNSQNPTGNDFVSQRFLALANGAYKYNPYILNQINQLGKEYGEVTDEDIYNIIRKQN
                     FSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLALITGEIGSMPDLKKPEDK
                     PDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCSMMVVFSIMLFLFVKR
                     NKKKNKNESQRR"
     gene            complement(2170..2913)
                     /locus_tag="POH73_002236"
     CDS             complement(2170..2913)
                     /locus_tag="POH73_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDI6888162.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     YTQIFGTQQTSPNTERLIVIARKIPLFIFFVSIVGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFAIATTVSSLIFALAHNDYKFIPIYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            3088..3372
                     /gene="groES"
                     /locus_tag="POH73_002237"
     CDS             3088..3372
                     /gene="groES"
                     /locus_tag="POH73_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDI6888163.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            3448..5064
                     /gene="groL"
                     /locus_tag="POH73_002238"
     CDS             3448..5064
                     /gene="groL"
                     /locus_tag="POH73_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDI6888164.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDATIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEVGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(5133..6305)
                     /locus_tag="POH73_002239"
     CDS             complement(5133..6305)
                     /locus_tag="POH73_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6888165.1"
                     /translation="MASYEKRGNTWRYRISLGKDAETGKYKYISNSGFKRKSDAKHHA
                     EMVERQLRNGDYIAPSTSTFKQVADDWISQYANEVKVSSVRAREKAINHAIERFNNKP
                     IQTINKHEYQRFVNDISAQYSKNYVDSIIASTNMIFKYAYDMKLIRIMPSEGIKRPKK
                     KISVEELEDTEIHKKFLEKDELFQFLEVAKNHHSPQNSFEVFCTLAYTGMRAGELLAL
                     KWSDIDFENNTINITKTYYNPNNNKKQFQILTPKTESSIGKISVDPHVIKLLKNYKTD
                     VQDTWKNELYVDNNFVFTDVNGYPLVIKKLQLWIKAILKKTDITNKQISTHSFRHTHC
                     ALLIEAGVHIKEIQERLRHKDINTTMNIYAKITNSYKKDASQKFSKLMENVSKDLF"
     gene            complement(6319..6993)
                     /locus_tag="POH73_002240"
     CDS             complement(6319..6993)
                     /locus_tag="POH73_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6888166.1"
                     /translation="MIRNRLSELLSERGLKISRVAKDVKIARSSLTSMAQNDSEMIRY
                     DAIDKLCSYLHISPSEFFEHNPINFDFTFDEEPNYKINDVFEGFEVTANITHAFSIEN
                     FDFEILVDVELDNRQKLNFDLDVSYKETEKITNSQHRFIFTIKNEDENIGLKKYVDSL
                     SAGLKNLLFKKINQKLSGYVSEIIVKNIDDIEELFPNKGEKSTTLHKEILQTDSRLSS
                     DIFKEY"
     gene            7166..7384
                     /locus_tag="POH73_002241"
     CDS             7166..7384
                     /locus_tag="POH73_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6888167.1"
                     /translation="MTILANTRKFKEAMFLKGFNLSDLSRETGVGISYLSQIINGKKI
                     PSPKLAKKMAEVLQVEVNELFEFEVKEA"
     gene            7389..7706
                     /locus_tag="POH73_002242"
     CDS             7389..7706
                     /locus_tag="POH73_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6888168.1"
                     /translation="MFNINIDEDEARELLEQAINARVDELAKEKYFMTYKELSNYLNL
                     SKPTIEELLINNGMKYYMVGSTYRFKKSDVDEFMEQLTAHMNIQNNDFKQVNIKKLLE
                     ARQ"
     gene            7703..>7856
                     /locus_tag="POH73_002243"
     CDS             7703..>7856
                     /locus_tag="POH73_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDI6888169.1"
                     /translation="MKIYLTYICLVSLLTILLLAISNMYVAFSVYAWLITLGCNLTGE
                     ITTCENK"
BASE COUNT         2721 a         1210 c         1376 g         2549 t
ORIGIN      
        1 tcctattctt aagtacacat tagcagcggc taatgtgtaa gaactactac ataatgaata
       61 actaatgatt ctatatcatt tctgtcccat tcccaacaat atattgatta tttttttatt
      121 acgaaacgat cttccactgg attaaatgtt ttttcgccag cagcttcacg aatatcacca
      181 aatggcattt gagcaataag tttccaactt ttaggaatat taaattcatt tgaagtcatc
      241 tcatcaacaa gtggattata gtgttgtaat gaagcaccta tgcctttagt agctaatgca
      301 gtccaaattg caaattgatg catggcattt gtttgagttg accatattgc aaaattatca
      361 tagtagtttg gcatttgttc ttgtaaacca cttacaacat cttgattttc ataaaacaaa
      421 attgtaccgt atgaatgttt gaagttatca attttttgtt cagttggctc gaaatcacga
      481 ttctctccca tgacttcttt taaaattgct tttgtgttat cccaaaattt attattgttg
      541 tcatttaata agagaacaat tctagttgat tgagaattaa atgatgaagg aacatgttta
      601 actgcatgtg caatcattga ttctaattcg tcatcgctaa ttgatattga atctttcaaa
      661 ttatatattg aacgtctttc ttccattgcg ttgtcaaaag tcattgcttt tttatctttt
      721 ttaaataaac ccataatcat tactccttct ttagtaaaga atacttaata gacttagtat
      781 aaaatttata ctcgaacttg taaagtaata tttacgaaaa tttcaagaat attaatattc
      841 attttcaaat tccaaatata aatgcatttt caacgcatat ttattatact tagattaata
      901 cttatatgaa aaagggaggt gtctcgtgaa atgtcatatc attggtttaa gaaaatgtta
      961 ctttcaacaa gtatgttaat tttaagtagt agtagtagtt tagggcttgc aacgcacaca
     1021 gttgaagcaa aggataactt aaatggagaa aagccaacga ctaatttgaa tcataatgta
     1081 acttcaccat cagtaaatag tgaaatgaat aataatgaga ctgggacacc tcacgaatca
     1141 aatcaagctg gtaatgaagg aactggttcg aatagtcgtg atgctaatcc tgattcgaat
     1201 aatgtgaagc cagactcaaa caaccaaaac ccaagtccag attcaaaacc tgacccaaat
     1261 aacccaaacc caggtccgaa tccgaagcca gacccagata agccgaaacc aaatccggaa
     1321 ccaaagccag acccagataa gccgaaacca aatccggatc caaaaccaga tccagacaaa
     1381 ccgaagccaa atccggatcc aaaaccagat ccaaatccga atccgaatcc aaaaccagac
     1441 cctaataagc caaatccaaa tccgtctcca aatcccaatc aacctgggga ttccaatcaa
     1501 tctggtggct cgaaaaatgg ggggacatgg aacccaaatg cttcagatgg atctaatcaa
     1561 ggtcaatggc aaccaaatgg aaatcaagga aactcacaaa atcctactgg taatgatttt
     1621 gtatcccaac gatttttagc cttggcgaat ggggcttaca agtataatcc gtatatttta
     1681 aatcaaatta atcaattggg gaaagaatat ggtgaggtaa ctgatgaaga tatctacaat
     1741 atcatccgta aacaaaactt cagcggaaat gcatatttaa atggattaca acagcaatcg
     1801 aattacttta gattccaata tttcaatcca ttgaaatcag aaaggtacta tcgtaattta
     1861 gatgaacaag tactcgcatt aattactggc gaaattggat caatgccaga tttgaaaaag
     1921 cccgaagata agccggattc aaaacaacgt tcatttgagc ctcatgaaaa agatgatttt
     1981 acagttgtaa aaaaacaaga agataataag aaaagtgcgt caactgcata tagtaaaagt
     2041 tggctagcaa ttgtatgttc tatgatggtg gtattttcaa tcatgctatt cttatttgta
     2101 aagcgaaata aaaagaaaaa taaaaacgaa tcacagcgac gataatccgt gtgtgattcg
     2161 ttttttatat tatggaataa aaatgtgata tataaaattc gcttgttccg tggctttttt
     2221 caaagcttca ggattaagta attggaatat aacgacaaat ccattttgta acatatgaat
     2281 aataattgga acagcaagtc gtttcgtcca tacatatgct aatgaaaaaa tgacgcccat
     2341 accaaagtat ataggaataa atttatagtc attatgcgct aatgcaaaga ttaatgagct
     2401 tacagtcgtt gcaatagcaa atgcgacgat acgattacct ttaattgcat taaataattc
     2461 gccaaagatt acttttctaa atacatattc ttctaataga ggaccaacga ttgatacgaa
     2521 aaagataaac aatggaatct tacgtgcaat aacgattaaa cgctcagtat ttggacttgt
     2581 ttgttgtgtg ccaaaaattt gagtatatac aatactaact accatttgat aaatcattac
     2641 cagtgcaaat ccaagcaatg cccatggaat gatatatttt ttaggttctt taacttctaa
     2701 ttctaatttt gttggatttt taatttttaa attaattaaa ataatcgtcg tggcggcgat
     2761 taaaaataga acaagttgta tgtaaatgac tgctttagtc agttctatgc cactatattg
     2821 tacaaatggt aattttttta caatgagaag cggtaaaaat tgagacaata tataaataat
     2881 aacagttagc aatgatgccc atagtcttgt cataattttc ctccaaatat ttgtttataa
     2941 tttattttat cgtaaataac ttgaagttac aaaacttaat tgaaaggtta tgacttgaag
     3001 ttttgaccaa atttgattat tataaatgta tgttagcact ctataaagtt aagtgctaaa
     3061 ctttaggttt tttaaggagg aacaatcatg ctaaaaccaa ttggaaatcg tgtgattatt
     3121 gagaaaaaag aacaagaaca aacaactaaa agtggtattg ttttaactga tagtgctaaa
     3181 gaaaaatcaa acgaaggcgt tatcgttgca gtaggaacag gacgcctatt aaatgatggt
     3241 acaagagtga ctcctgaagt gaaagaaggg gaccgtgtcg tgttccaaca atatgcaggt
     3301 acagaagtta aacgagataa tgagacttat ttagttttaa atgaagaaga tattttagcg
     3361 gtaattgaat aatatagaat ataattcata gatagattgt aaagaacgaa aatgaaatat
     3421 gactaaacaa atggaggttt atcacttatg gttaaacaat tgaaattctc tgaagatgca
     3481 cgtcaagcaa tgttacgtgg tgttgaccaa ttagcaaatg cagttaaagt aacgattggt
     3541 cctaaaggac gtaatgttgt attagataaa gagtttacag cacctttaat tacgaacgat
     3601 ggtgtaacga ttgctaaaga aatcgaatta gaagatccat atgaaaatat gggggctaaa
     3661 ctagttcaag aagtcgcaaa taagacaaat gaaattgctg gtgacggtac gacaactgca
     3721 acagtattag ctcaagcaat gattcaagaa ggcttgaaaa atgttacaag tggtgcaaac
     3781 ccagttggtt tacgacaagg tatcgacaaa gcagttaaag ttgctgttga agcgttacat
     3841 gaaaattctc aaaaagttga aaataaaaat gaaattgcgc aagtaggtgc gatttcagca
     3901 gcagatgaag aaattggacg ctatatttca gaagctatgg aaaaagtagg taacgatggt
     3961 gtcattacaa ttgaagaatc aaatggcttg aacactgaac ttgaagtggt tgaaggtatg
     4021 caatttgatc gtggttatca atcaccgtat atggttactg attcagataa aatggttgct
     4081 gaattagaac gcccatatat tttagtaaca gataaaaaaa tctcttcttt ccaagatatc
     4141 ttacctttat tagaacaagt ggttcaatct aatcgtccaa tcttaattgt agctgatgaa
     4201 gttgaaggcg atgcattaac aaatatcgtg ctaaaccgta tgcgtggcac atttacagct
     4261 gttgcagtaa aagcacctgg tttcggtgat cgtcgtaaag caatgttaga agatttagct
     4321 attttaactg gtgcgcaagt gattacagat gatttaggct tagaccttaa agatgcaacg
     4381 attgatatgt taggtactgc aagtaaagta gaagttacta aagataatac aactgttgta
     4441 gatggtgacg gtgacgaaaa cagcattgat gcacgtgtaa gccaattgaa atctcaaatt
     4501 gaagaaactg aatctgactt tgatcgtgaa aaattacaag aacgcttagc taaattagca
     4561 ggcggtgttg cagttatcaa ggtaggtgca gcaagtgaaa cagaacttaa agaacgtaaa
     4621 ttacgtatcg aagatgcttt aaactctaca cgtgcagcag ttgaagaagg tattgttgca
     4681 ggtggtggta ctgcactagt aaatgtttac caaaaagtaa gtgaaattga agctgaaggc
     4741 gacattgaaa caggtgtgaa tattgtactt aaagcattaa ctgcaccagt tcgtcaaatt
     4801 gctgaaaatg caggattaga aggctctgtc atcgttgaac gcttgaaaaa tgcagaacca
     4861 ggtgttggtt ttaatgctgc tacaaacgag tgggttaata tgttagaagt aggtatcgtt
     4921 gatccaacta aagtaacacg ctcagcatta caacatgcag caagtgttgc ggcaatgttc
     4981 ttaacaactg aagcggttgt ggcatcaatt ccagaaaaaa ataatgacca acctaacatg
     5041 ggtggcatgc caggaatgat gtaaaatgac tgttaaacgc cgattttata acgtttgtaa
     5101 tattgggtgg tcataatttg gtcatagaaa ttttaaaata aatcttttga gacgttttcc
     5161 atgagtttac taaacttttg agaagcgtct tttttgtatg agttcgtaat cttagcgtag
     5221 atgttcatag tggtatttat atctttatgg cgcaagcgtt cttgtatttc cttaatatgc
     5281 acaccagcct ctataagtaa cgcacaatga gtatgacgaa atgaatgagt gcttatttgt
     5341 ttattagtta tgtcagtctt tttaagtata gcttttatcc ataattgtag ttttttaatt
     5401 acaagagggt agccgttaac atcagtaaaa acgaaattat tatctacata caattcgttt
     5461 ttccatgtgt cctgcacgtc ggttttataa tttttaagta atttaatcac atgaggatcc
     5521 actgaaattt ttccgattga gctttcagtt tttggtgtaa gtatttgaaa ttgcttttta
     5581 ttgttattcg gattgtaata agtctttgta atattgattg tgttattctc aaagtctata
     5641 tcagaccatt tcaatgccaa taattcacct gcacgcatgc ctgtatatgc taatgtacaa
     5701 aacacctcaa agctgttttg gggtgaatgg tgatttttag caacctccag gaattgaaat
     5761 aattcatctt tttcaagaaa ctttttatgt atctcagtat cttctaattc ttccacacta
     5821 attttctttt taggtcgttt aataccctcg ctaggcatta ttcttattaa tttcatatcg
     5881 tatgcgtact taaatatcat atttgtagag gctataatgc tatcaacata attcttgcta
     5941 tactgtgcgc ttatatcgtt tacaaaacgt tgatattcat gtttattgat agtttgtatt
     6001 ggtttattgt taaagcgttc tatggcgtgg tttatggctt tctcgcgtgc tctgacacta
     6061 cttactttta cttcgttagc atattgtgat atccaatcgt cagcaacctg tttaaatgta
     6121 gatgtggacg gtgcgatata atcgccattt cttaactgac gctcaaccat ttcagcgtga
     6181 tgtttagcgt ctgatttgcg tttaaaacct gagtttgaaa tatatttata tttgcccgtt
     6241 tctgcgtctt ttcctagtga tatacgatag cgccatgtat ttccgcgttt ttcataactt
     6301 gccatttgat cacctcgatt aatattcttt aaaaatatca ctagataaac ggctatcagt
     6361 ttgtaaaatt tctttatgta gagtcgtact tttttcgcct ttatttggaa aaagctcttc
     6421 aatatcgtct atatttttta ctattatttc agaaacatac ccacttaact tttggttaat
     6481 ttttttaaat aacaagtttt taaggcctgc agataaacta tcaacgtatt tttttaatcc
     6541 gatattttca tcttcatttt taatcgtgaa aataaatcta tgttgtgaat tagttatctt
     6601 ttcagtttct ttatatgaga cgtctaagtc aaaatttaat ttttgcctat tatctaattc
     6661 gacgtctact aaaatttcaa agtcaaaatt ttcaatcgaa aaagcgtgag taatgtttgc
     6721 agttacttca aatccctcga aaacatcatt aattttataa ttcggttctt catcaaaagt
     6781 aaagtcaaaa ttgatcggat tatgttcaaa aaattctgaa ggagatatgt gcagataact
     6841 acataattta tctatagcat catatcttat catttcagaa tcattttgtg ccattgaagt
     6901 aagtgaactt cttgctattt ttacatcttt tgcaacacga gatattttta gtcctctttc
     6961 tgacagtagt tcagacaatc tatttctaat cattacaaac ctcctaatta tgttaataat
     7021 agcatttttt tggacgttta tgtacaaaaa aataaaaaat gattgagaag tcagtcgaaa
     7081 aactattgca aaagaaaaac gattatgtat aataaagtta taaattgatt gagaagtcag
     7141 tcaaaaacga aggaggattt taattatgac tattttagcg aatactagaa agtttaaaga
     7201 agccatgttc ttaaaaggct ttaatttatc tgatttatca cgtgaaacag gtgttggaat
     7261 ttcttattta agccaaatta ttaatggtaa aaagattcca agccctaaat tagctaagaa
     7321 aatggcagaa gttttacaag ttgaggtaaa tgaattattt gaatttgaag taaaggaggc
     7381 ataaaccaat gttcaacatt aatattgatg aagatgaagc acgtgagtta cttgagcagg
     7441 ctatcaatgc acgtgtggac gaattagcga aagagaaata ttttatgact tacaaagagt
     7501 tgtctaacta tctgaattta agtaagccta ctattgaaga attacttatt aataatggca
     7561 tgaaatatta tatggtcgga tctacgtaca gattcaaaaa gtctgatgta gatgaattca
     7621 tggaacagct tactgctcat atgaatatcc agaataacga ctttaaacaa gtcaatatca
     7681 aaaagttatt ggaggcaagg caatgaaaat ctacttaact tatatctgct tagtttcatt
     7741 gttaacaata ttattactag caatatctaa catgtatgtt gcttttagcg tttatgcttg
     7801 gctaataact ttaggatgta atttaacagg agagattaca acgtgcgaaa acaagt
//
LOCUS       DAMZPP010000129         7825 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMZPP010000129 DAMZPP010000000
VERSION     DAMZPP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7825
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.129"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..882
                     /locus_tag="POH73_002244"
     CDS             <1..882
                     /locus_tag="POH73_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDI6888170.1"
                     /translation="EEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPM
                     PNARQTKEEVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQR
                     TNKKQKITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQ
                     FTPEIFMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFE
                     HRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFA
                     TRWFEEKFPEIKLYSLL"
     gene            1184..1987
                     /locus_tag="POH73_002245"
     CDS             1184..1987
                     /locus_tag="POH73_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDI6888171.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            2206..4530
                     /locus_tag="POH73_002246"
     CDS             2206..4530
                     /locus_tag="POH73_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDI6888172.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQGSKSAYN
                     KDHYLTDDVSKKQNSLDSVDQDTEKSKYYEQNTEATLSTNSTDKVESTDMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDFESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNSVPILSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHTPSISDDKDYVMREDHIVDDNPDNDINTPSLSKIDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSSASHRSTEKRNMAINDHDKLNGQKPNTKTSANNNQKKATSKLNK
                     GRATNNNYSAILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNAVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKELETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLLGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            4547..5905
                     /locus_tag="POH73_002247"
     CDS             4547..5905
                     /locus_tag="POH73_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDI6888173.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVVAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMVLMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            6044..7555
                     /locus_tag="POH73_002248"
     CDS             6044..7555
                     /locus_tag="POH73_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDI6888174.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EEIGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLNGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNLEPGQRVMLDQIDSTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYLISCSDNFVVKNR"
     gene            complement(7737..7825)
                     /locus_tag="POH73_002249"
     tRNA            complement(7737..7825)
                     /locus_tag="POH73_002249"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7788..7790),aa:Ser,seq:gct)
BASE COUNT         2814 a         1054 c         1484 g         2473 t
ORIGIN      
        1 gaagaagcgg gtgaagcctt tgataatgac gcgctgttga gtggagactt aacacctgta
       61 tttttcggtt cagctttagc taactttggt gtacaaaatt tcttgaatgc atatgttgat
      121 tttgcgccaa tgccaaatgc gagacaaaca aaagaagagg ttgaagtaag cccgtttgat
      181 gattcatttt caggatttat ctttaaaatt caagccaaca tggaccctaa acaccgtgat
      241 agaattgcct ttatgcgtgt cgttagtggt gcatttgaac gtggtatgga tgtcactttg
      301 caacgtacta ataaaaagca aaagatcaca cgttcaacgt catttatggc agacgataaa
      361 gaaactgtga atcatgctgt agcaggcgat atcattggac tatatgatac aggtaattat
      421 caaattggag atactttagt tggtggaaaa caaacctaca gtttccaaga tttaccacaa
      481 tttacgccag aaatttttat gaaagtttct gctaaaaacg tcatgaaaca gaagcatttc
      541 cataaaggta ttgaacaatt agttcaagaa ggtgcgattc aatactataa aacattacac
      601 acaaaccaaa ttattttagg tgctgttggt cagttacaat ttgaagtttt cgaacataga
      661 atgaaaaacg aatataatgt tgatgttgtt atggagccag taggccgtaa aattgcacgt
      721 tggattgaaa atgaagacca aattacagat aagatgaaca catcaagatc gattttagtg
      781 aaagatagat atgacgattt agtattctta tttgaaaatg aatttgcaac aagatggttc
      841 gaagagaaat tccctgaaat taaattgtat agtttacttt aacggctcaa ttgtataatc
      901 gaatttgtta cattaaaaat aattgtttcc ttgaagaaaa ataaattgta tattttaaaa
      961 gaaaaaggta tactatgatg tatcaaatga ataacctatg gcattttgtc agaggggagt
     1021 aacttaagaa tcatgaccgt ataaatgatt cgacacttta tcgtcattac gaagatatct
     1081 tccggtaaag tgggcaatta aaattgctta gtgagacctt tgctatttat ttagcatagg
     1141 tctttttgtt tgtacttaac ttatttattt aaaggagttg tacatgttaa tggatccaag
     1201 tttgatctta ccttatttat gggtacttgt cgttttagta tttttagaag gcttattagc
     1261 agcagataac gcaatcgtta tggctgtaat ggttaagcac ttaccacccg aacaacgtaa
     1321 aaaagctttg ttttacggtt tgctaggtgc atttgtattt agatttttag cattattctt
     1381 aattagtatt atcgcgaact tttggtttat tcaagctgca ggagcggttt acttaattta
     1441 tatgtcaatc aaaaatctat ggcagttctt taaacaccca gaaattgaaa gtcctgaagc
     1501 tggagatgat catcattatg atgagtctgg tgaagagatt aaagcaagta acaaatcatt
     1561 ctggggaact gtgttgaaaa tagaatttgc agatatcgca tttgccattg attctatgct
     1621 tgctgcttta gctattgctg taacacttcc taaagttggt attcactttg gtggtatgga
     1681 cttaggtcag ttcgtagtca tgttcctagg tggaatgatt ggtgttattc taatgcgtta
     1741 tgcagcaaca tggttcgtag agctattaaa caaatatcca ggacttgaag gtgcagcctt
     1801 cgcgatcgtt ggttgggtag gtgttaagtt agttgtcatg gtattagcgc acccagacat
     1861 cgctgtattg cctgagcact tcccacatgg cgtattatgg caatctattt tctggacggt
     1921 actaattgga ttagtaatta tcggttggtt aggttcagtt gttaaaaata aaaaatcgca
     1981 taaataaatg atgtgaagcg gacaatctta atttagttta aggttgtcct ttttcattta
     2041 attaagtgat ctatgaaaaa tggattttga agaatatgta tcaaaagatg cgatatagta
     2101 ttaagaaaat gtgcctttta tatttagcat tttttcaata gaaattatat agattttaaa
     2161 gcaaattaag tgttaatgtg tcataatgaa aagtgatttt attgaatgga gtggacatta
     2221 gtggatattg gtaaaaaaca tgtaattcct aaaagtcagt accgacgtaa gcgtcgtgaa
     2281 ttcttccaca acgaagacag agaagaaaat ttaaatcaac atcaagataa acaaaatata
     2341 gataatacaa catcaaaaaa agcagataag caaatacata aagattcaat tgataagcac
     2401 gaacgtttta aaaatagttt atcatcgcat ttagaacaga gaaaccgtga tgtgaatgag
     2461 aacaaagctg aagaaagtaa aagtaatcag ggtagtaagt cagcatataa caaagatcat
     2521 tatttaacag acgatgtatc taaaaaacaa aattcattag attcagtaga ccaagataca
     2581 gagaaatcaa aatattatga gcaaaatact gaagcgactt tatcaactaa ttcaaccgat
     2641 aaagtagaat caactgacat gagaaagcta agttcagata aaaacaaagt tggtcatgaa
     2701 gagcaacatg tactttctaa accttcagaa catgataaag agactagaat tgattttgag
     2761 tcttcaagaa ctgattcaga cagctcgatg cagacagaga aaataaaaaa agacagttca
     2821 gatggaaata aaagtagtaa tctgaaatct gaagtaatat cagacaaatc aaattcagta
     2881 ccaatattgt cggaatctga tgatgaagta aataatcaga agccattaac tttgccggaa
     2941 gaacagaaat tgaaaaggca gcaaagtcaa aatgagcaaa caaaaactta tacatatggt
     3001 gatagcgaac aaaatgacaa gtctaatcat gaaaatgatt taagtcatca tacaccatcg
     3061 ataagtgatg ataaagatta cgttatgaga gaagatcata ttgttgacga taatcctgat
     3121 aatgatatca atacaccatc attatcaaaa atagatgacg atcgaaaact tgatgaaaaa
     3181 attcatgtcg aagataaaca taaacaaaat gcagactcat ctgaaacggt gggatatcaa
     3241 agtcagtcaa gtgcatctca tcgtagcact gaaaaaagaa atatggctat taatgaccat
     3301 gataaattaa acggtcaaaa accaaataca aagacatcgg caaataataa tcaaaaaaag
     3361 gctacatcaa aattgaacaa agggcgcgct acaaataata attatagtgc cattttgaaa
     3421 aagttttgga tgatgtattg gcctaaatta gttattctaa tgggtattat tattctaatt
     3481 gttattttga atgccatttt taataatgtg aacaaaaatg atcgcatgaa tgataataat
     3541 gatgcagatg ctcaaaaata tacgacaacg atgaaaaatg ccaataacgc agttaaatcg
     3601 gtcgttacag ttgaaaatga aacatcaaaa gattcatcat tacctaaaga taaagcatct
     3661 caagacgaag taggatcagg tgttgtatat aaaaaatctg gagatacgtt atatattgtt
     3721 acgaatgcac acgttgtcgg tgataaagaa aatcaaaaaa taactttctc gaataataaa
     3781 agtgttgttg ggaaagtgct tggtaaagat aaatggtcag atttagctgt tgttaaagca
     3841 acttcttcag acagttcagt gaaagagata gctattggag attcaaataa tttagtgtta
     3901 ggagagccaa tattagtcgt aggtaatcca cttggtgtag actttaaagg cactgtgaca
     3961 gaaggtatta tttcaggtct gaacagaaat gttccaattg atttcgataa agataataaa
     4021 tatgatatgt tgatgaaagc tttccaaatt gatgcatcag taaatccagg taactcgggt
     4081 ggtgctgtcg tcaatagaga aggaaaatta attggtgtag ttgcagctaa aattagtatg
     4141 ccaaacgttg aaaatatgtc atttgcaata cctgttaatg aagtacaaaa gattgtaaaa
     4201 gaattagaaa caaaaggtaa aattgactat cccgatgtag gtgttaaaat gaagaatatt
     4261 gccagtctaa atagttttga aagacaagca gttaaattgc taggaaaagt taagaacggt
     4321 gttgttgtag atcaagttga caacaatggt ttagcagatc aatctggtct gaaaaaaggt
     4381 gatgtaatta ctgaattaga tggcaaactt ttagaagatg atttacgctt taggcagatt
     4441 atatttagtc ataaagatga cttgaaatca attacagcga agatttatag agatggtaaa
     4501 gaaaaagaaa ttaatattaa actaaaataa tgttgaggtg aacaacgtgt caatttttag
     4561 ccagttttta aaaagatcaa gccctcaaca aggtattgta ttgtattata tcgtcgcaat
     4621 tgtcgttgca tttttattat taaacttacc gtatgttcat aaaccaggtg tagaagtaaa
     4681 tccaattgac acattatttg ttgccgtatc cggaattagt gttactggat tgtctccgat
     4741 aagtattgtc gatacctatt ctacatttgg acaattaatt atcctcgtga tattaaatat
     4801 tggtggaatt ggcgtcatgg caattggtac gatgttatgg gtggtactag gtaaacatat
     4861 tggaattaga gaacgtcagt taattatgtt agataataac aaaaacacaa tgagtggtac
     4921 cgtcaaattg attattgata ttgtaaaatc aatatttgta atcgaactcg taggagccat
     4981 gttattagca ttttactttt atcgagataa tccagattta aaatatgcaa tcatgcaagg
     5041 tgtttttgtt tctatttctg ccactaccaa tggtggatta gatattacag gtaagtcatt
     5101 aattccttat gcacatgatt attttgtaca agcgatagtt atatttttaa taattttagg
     5161 atcaatcggc ttcccagtat tattagaggt taaagcttac attcaaaata gggttactaa
     5221 ttttagattt tcattattta ctaaaattac gacatcaaca tatttattcc tatttattgt
     5281 tggggtatta gccattctat tatttgaaca taaccatgcg ttcaaaggat taagttggca
     5341 tcaatcgtta ttctattcgc tgtttcaatc agcgactaca agaagtgcgg gtcttcaaac
     5401 aattgatgtg acaacactaa gtgaccctac gaatattatc atgggtattt taatgtttat
     5461 aggttcttcg ccaagttcgg ttggtggcgg tattcgtaca acaactttcg ctattttaat
     5521 tttgttttta attaacttta gtaataatgc cgataaaaca tccataaaag tatacaatag
     5581 agaagtacac attatggata ttcaacgttc atttgcagta tttacaatgg cgacaatttt
     5641 aacattttta ggaatgctaa ttatatcagc tactgaaaat ggtaagctta catttttaca
     5701 agtatttttt gaagtcatgt ctgcatttgg aacttgtgga ctatcgcttg gtgtcacaag
     5761 tgatattagt gatatttcca aggtcgtact aatggtactc atgtttatag gacgtgttgg
     5821 cttaatatca tttatcatta tgatagcagg acgtcgagaa ccagataaat tccattatcc
     5881 aaaagaacgt attcaaatag gataatataa tagcaatcta agtttagtta atgtagattt
     5941 taactggaac ttagattgct tttttagttt gtatttttaa cttatattat aagacgattg
     6001 gatttgaaaa tggtaaacta gtaacaatga gaggtgtaac atgatggaaa aaaatgaaaa
     6061 cattaatgta gagattttaa ctacgtcaga tatgcatagt catttcttaa atggtgatta
     6121 tggttcaaat atttatagag ctggtactta tgttaaccaa gtaagagcac aaaatcatcg
     6181 cgtcatttta ttagatagtg gcggaagttt agctggctcg ttagcggcct attattatgc
     6241 tattgttgca ccttataaac gacatccaat gataaagttg atgaacagaa tgcattatga
     6301 tgctagcggt gtgagtccaa gtgaattcaa gtttggttta tcatttttaa ctcgttcaat
     6361 tgctttggca cgttttccat ggttatcagc aaatattgaa tacaatgtta ctaaggagcc
     6421 ttatttttca actccatatt gtattaaaca ttttggtgac ttaaaaattg ctatcgtagg
     6481 cgttacagca gatggtttaa tggaaaatga gtattctgaa atggagcaag atgtatctat
     6541 tgaaaagaca ttagtggcat caaaacgatg gattagatat atccatgaag ttgaagagcc
     6601 ggattttttg attgtaattt atcatggtgg attgaataaa attagtaata gcacgaaaaa
     6661 taaaaaggca agttcgaatg aagctgaaaa attaatggaa gaaattggtg ttatagattt
     6721 aatgattaca gctcatcagc atcaaacaat agtaggtcaa gatcatgaaa cgtattatgt
     6781 tcaggctggt caagatgcaa aagagcttgt acatctttcg attaatttta aaaagagaac
     6841 aacaacttat gatgttgaaa gcattgattc taaagtgatt gacttaaatg agtatgaaga
     6901 ggaccaagaa ttattagatt taacattcta tgatagaaaa gcagtggctt attggtcaca
     6961 ggaaatcata agtgataaag gtttgatgtt atcagtaaat gggttacaag atttagtctg
     7021 tcaaacacat ccattttcgc aattattaca tgatgcaatt caccttgcat ttgataatga
     7081 tataacatgt gtccacgtgc ctatgaacgg agagaagggg ttgaatggac agattcgaaa
     7141 tgaagatttg tatcatgcat acccttatcc agataagcca atggatatga caattagtgg
     7201 tcaaaatatc aaagatatat tggagtatag ttattcacat ttagattttg ttaacgagca
     7261 attaagctta acaattattg atgaaacatt atgtacaatg tggcaaggat tcaattatga
     7321 gattgatatg aatctagaac ctgggcaacg agtaatgtta gatcaaattg attcgactaa
     7381 gagttataga gttacaatga ctgactattg ttatcgtaac tacaagaatt atttaaaaaa
     7441 tgctattata catgaatcat acgatgaaac aatgagtaca ttaattgcag agaagttaag
     7501 agatccgaat tatcttattt catgtagtga taattttgta gttaaaaaca ggtgaatatt
     7561 gctatgtaat aaattaatta taagtttaac aagtataaaa acctttctta gagaagatta
     7621 tctaggaaag gtttttagaa ttataaaatt gatataaaag aacagtgaaa ttctcattca
     7681 ttatatatat aagtaaacaa aaaagcttaa atggtatata ccatttaagc aagtaacgga
     7741 aacggaggga ttcgaaccct cgcgccgctt tcgcgaccta cacccttagc aggggcgcct
     7801 cttcagccaa cttgagtacg tttcc
//
LOCUS       DAMZPP010000130         7744 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMZPP010000130 DAMZPP010000000
VERSION     DAMZPP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.130"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..489)
                     /locus_tag="POH73_002250"
     CDS             complement(<1..489)
                     /locus_tag="POH73_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HDI6888175.1"
                     /translation="MMFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFESFMRKYSYHLSSSRLISGSSTVYEEIESMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPN"
     gene            complement(467..1474)
                     /gene="bioB"
                     /locus_tag="POH73_002251"
     CDS             complement(467..1474)
                     /gene="bioB"
                     /locus_tag="POH73_002251"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDI6888176.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSREIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNDVSRA
                     N"
     gene            complement(1476..2834)
                     /gene="bioA"
                     /locus_tag="POH73_002252"
     CDS             complement(1476..2834)
                     /gene="bioA"
                     /locus_tag="POH73_002252"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDI6888177.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSQKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKCKLLSQIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(2812..3498)
                     /gene="bioD"
                     /locus_tag="POH73_002253"
     CDS             complement(2812..3498)
                     /gene="bioD"
                     /locus_tag="POH73_002253"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDI6888178.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMANQIFLNKQCVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTGSYIEKDNQITIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            complement(3553..3720)
                     /locus_tag="POH73_002254"
                     /pseudo
     CDS             complement(3553..3720)
                     /locus_tag="POH73_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3951..5684)
                     /locus_tag="POH73_002255"
     CDS             complement(3951..5684)
                     /locus_tag="POH73_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6888179.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLVAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNGSKRINTKIEMQFIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGDPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            complement(5709..7472)
                     /locus_tag="POH73_002256"
     CDS             complement(5709..7472)
                     /locus_tag="POH73_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6888180.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNAYTINSIMVYIFIVLIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFIAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGIDIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATAYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
BASE COUNT         2455 a         1440 c          993 g         2856 t
ORIGIN      
        1 attagggtat ttctctaacc ttttttctaa atcagcaata tctaaatgct tatatatcac
       61 tttttctaaa ccacttagct taataccgtc aataatactc gcatgatttt cttgatctga
      121 aaatacgaca caatttgtat ttttgaaaat attaaataac gccaaattag catcataacc
      181 actatttaag atagtacatg cactatatcc gagccagcct gctaacattg attcaatctc
      241 ttcataaact gtcgaacttc cactaatcaa tcttgaactt gataagtgat aactatactt
      301 ccgcataaaa ctttcgaaat catccttatc aaacgctatt tgacctaatc ctaaataatc
      361 attagatgta tagttcgtac atcgcttatt ttctacttca atatactgtc tatctatata
      421 ccctaccgat ttaagcgacc gatataaccc tttctgttgt aataaatcaa tttgctcttg
      481 aaacatcatt cttgttttcc ttattttcac aagtgtcata atcaatttca aagcccaaat
      541 cattaatcat atcgtagtct aattggttcg gttgcccacc agtaattaga taatcaccga
      601 caaatattga attcgccgct tttaatgcta atggctgtaa cgaacgtaag ttgacctctc
      661 ttcctccagc aatacgaatt tctttcgtag gattgattaa tcggaataat gctacgattc
      721 ttaaacattt cattggtgtt aaatcatcca tgcttccaaa ctttgtacct ttgattggat
      781 gcaaaaaatt aatcggaata ctgtcggcat ccatttcttt caaagcaaat gccatatcaa
      841 caatatcttg attagattct cccataccac aaatcacgcc agaacatggt gatatattat
      901 tcgctttcat tagttctatc gtatctgttc tatctttata actatgcgtt gtcacgacgt
      961 tatcatggta attttcactt gtattaatat tatggttata tctgtctaca ccagctgact
     1021 taagtttctt agcttgttcg tcattcgtta atcctaaaca tgcacatatt ttcagttgcg
     1081 ggtgttgaga tttaatcgtt cttacagtgt tactaatatg atcaacttct ttatcgctcg
     1141 gtcctctacc actcataaca atacaatatg ttccaatatg attatcatgt gccacctttg
     1201 ctccatcaat aatttgttcc tctggaatta gagcatatcg ctgtttttgt ttaatttctc
     1261 gtgattgtcc acaataccca caattctcag gacatatacc acttttagca tttaaaatca
     1321 tgtttaattt tactttttta ccgaaataat gttttcttaa aatatacgcc tcatttaata
     1381 aatctaaggt atcaatatta gtatcctcat aaattttcaa tacagtctct tttgttaatt
     1441 gttccccttg taatatgcgt ttagccaaat tcatattaac acttcctatc taaaatgtta
     1501 tgtactgcct ttttaaaaat acgtaccatc gtttttactt ctttatttga catacttaac
     1561 actggcacaa acgtaatgac attttctaaa tttctaatca ttagcccgtt ttctttacaa
     1621 ttacgtacaa tacgttctac tttttcaatt tctaacggcg ttttactatc tttatctgta
     1681 actaattcaa ctccaaacat taagcctcgt cctcttacat caccaacatt tcgatgacgc
     1741 ttcagcgcat gtaactgttt ctcaagtgtt gatgatgtcg tttcaatttg tgacaataac
     1801 ttacattttt cataaagtct tatattttct aatgcaaccg tacaaacgat ttgatttccg
     1861 gtgtatgtat gaccatggaa aaaggtattc acaccatgcg aatcacttaa aaatgcattg
     1921 tatatttttt gagatgtcaa tgtagctgca agtggtaagt agccacctgt aatcgcctta
     1981 cctaaacaca taatatccgg ttgaacatct tcatgattgc atgcaaacat ctttccagtt
     2041 ctcccaaaac caactgctac ctcatcacaa attaataaga catcgtattt tttgcacaat
     2101 ttctcgactt ctttcaaaaa gcctttagga tgaacaaaca agcctgtcgc accttgaatc
     2161 aacggttcca atataaaccc tgcgatttca tcatttcttt caattagaat ttgctctata
     2221 ttcgttaaaa tagctgtcat catttcattt tcagtatcgt aattactgcg atagaaagat
     2281 gggctttcta cttgaatatt ttcaaatatt aagtctttaa atattttatg aaaggtcttg
     2341 ataccaccaa cacttaccgc accaatcgta tccccatgat aaccgtgatt tagcgttata
     2401 aacttgtttt tcttggcata tttttctcta tcaatatttt tccaatactg atatgccatc
     2461 tttattgcga tttcaacaga cgcactgcct gtatcagaat aaaatacttt tcttagatta
     2521 cttggcgtga tttcgattaa tttttccgca agttctattg acggaatatt tgatgatcct
     2581 agcagcgtag aatgggcaat tttattgagt tgctttttaa ttaccttatt caagtatttg
     2641 ttattatgac catgcacatt gacccacaac gatgcataac catctaaata tttattgcca
     2701 ttcgtatcgt aaaggtaact accctttcct ttttcaatga tgattgcttc ttctttgcta
     2761 tatacaccca tttgtgtaaa tggatgccaa acatattctg agtctttttg tttaagttgt
     2821 tgtgtgtaat tcatttttaa ctcctattaa ttgttttaaa aatgcttctg agaaattttc
     2881 atacgtggca tgttcttcaa atgtatagac tgttttattt gttaattttc caatcgtaat
     2941 ttgattgtct ttttcaatat agctgcctgt atagcgattc attattaaaa aattactcgc
     3001 cgatacattc tgattaacat aatcttggtg aacaatggca tcgctaatag cacctaattt
     3061 tgatggcaac acactgatga cacaatctgc acaatcattg attagatctt tagtcatgta
     3121 gaaatcatct gtaccttcat atattggtac ggcaattccc ccagcaccct caattaagat
     3181 aaaatcaaat tccttatcta aaacttttac cttatctaat acacactgct tatttagaaa
     3241 aatttgattt gccattttaa atgcaaggtg tggtgataca ggttgcttaa aagtataaag
     3301 tgacgttatg tcatagctta aatcacattc atttttaaat acttctaaat ctggaaatgt
     3361 cccgtcttgg cgttcctcag tttgaaatgg tttaaaaata caaactcgat gaccacgtgt
     3421 tttcaaagca tggtataaat gctttgtaac ataggttttg cctacatcag tattcgtact
     3481 tgtaataaaa atcctcataa aaaataagca cctcaatccg aatgtaaact tttataatta
     3541 ataagtttac atttaaggcg cgtgtttttc aagaaaacat gaatttaaac ttctcagtat
     3601 aactataaat tttagcaaaa ttttgacctt gctattgaaa tgactaaggc ttcatacgga
     3661 attcgggcag atataaacag cgattcgata aaatatgtca gttaaaatta ataattgcat
     3721 tatttcttaa taaaaagaca tcctagcttt accatgttgt gaaacaatcc attaattgta
     3781 atattacgtt gagcgccttg ttcatcaatg ctataattag ttacataata aattgaacat
     3841 ctaaatacac caaatcccct cactattgcc ctagtgaggg gatttatttt gctgttgatt
     3901 atctatcacc ctttttattt ccaaattctg attccatatt aaactattat ttataatttc
     3961 cagcctgctg atttactttt tattcttata aagtcttgat acattcctgg ttttcgaatt
     4021 aattcatcat gactaccttt ttgaattatt tcaccttcat tgagcactat aatctgatct
     4081 gcatttttaa tagtttcaag tttatgtgca attgtaatta ctgttttgcc tttacttaat
     4141 tcattaattg ccgtttgaat cagctgttca ttttcagggt caatacttgc agttgcttca
     4201 tctaaaataa ttattggtgc atcttttaat atcgccctag caatagaaat cctttgcttt
     4261 tctccgccag ataaattact acctttttca tttagcattg tttgatatcc ctcaggtaat
     4321 gacatgatga agtcgtggca acatgcttgc ttcgctgcac gaataatttc ttctttcgtt
     4381 gcacctggat cgccaaacaa tatgttattt tcaatcgtat cattaaataa atacaccttt
     4441 tgaaatactg cactaatttt cgacattaac gtacttaatg tcatatcttt aatatcaaca
     4501 ccatcgatgc gaatatttcc atcatcgata tcataaaagc gcaagagtaa gtgacataat
     4561 gtagatttcc cacttcctga aggtccaatt attgctgttg atgtttgtgt aggtatctca
     4621 aaattcacat tctttatcac ttgtttatca tcatatgaaa aattcacgtt ttgaaaagcg
     4681 atattttcat ttttaatcgt caaatttttt ccattctcat ccagttctgg agcatttttt
     4741 atcttttcaa tatcatctat cgtcatatct atcatttcaa gtacgtgtgc agcactatta
     4801 atattttcaa cactatcaaa tatcacaaat gaaaaaattg aaatcataat aagggtaggt
     4861 aaatcaatac tatgattcat atataataag catgcaacta atacaatcat tattgaaaca
     4921 acttttaaac taagtaaatg caataaattg aatgggataa attgcatttc tatttttgta
     4981 tttatacgtt tacttccatt gactgcttgg ttaaaacttt taagactcgt attttctttc
     5041 gcgaatgatt ttattacttg aataccacga ataacctcca ataccttttc cactaattgg
     5101 ttttgtacat tatgatacgc tggcgcattt tgtcgactct ttctttctaa taattgaatc
     5161 gcaaaaaatg atagtaatac gccaatgcat gctaataatg atacttgcca cgaaactaca
     5221 agtagagaca atatgagtac tgtaattaat atgtatccat taacaacaac gtccaccatt
     5281 ttcatagcaa agttttctaa aaaggttaaa tctgttgtta ctattgttgt taactcattt
     5341 gaatgatgcg aattaaaata acctaacctt acatttttca atttatcccc tatatctaaa
     5401 cgttctttcg cactcatttc ataagcgatg ctctcatggc ttttgctttt gaaatatgct
     5461 gtaataaatc gtccaatcac taataaaacc atgataatta caacattcaa tatatctttc
     5521 atataaatag gtttatgaga tagcacatta ttaaatattt ttgcaactaa aaagataggt
     5581 aacgcaataa aaatagcatt taaaaatgac atgctaaatc ctaaaatcat tcttgcttta
     5641 tatggtctta tccagtttaa aattttaaat gtaatttgaa acatatttga cctccttttc
     5701 tcattagttt aattatgtcc agtattgatt ccccaatctt ttgtatgcat gtgcgtatcc
     5761 cacattttct tataattacc gtttaatttt agcaataagt gatgtgaccc tttttctaaa
     5821 atttgttgtt tacctaaaac aataatttga tctgcatgtt gaatcgtaga taaccgatgt
     5881 gcaataacaa tcaatgtttt atcctgcgtc aacacattta atgcttcttg aattttttgt
     5941 tcgttgtctg gatcaacgta agcagtcgct tcatctaata caatgatagg cgcatctttt
     6001 aatatcattc ttgcaatagt gactcgttgt ttttcaccac cagacaattt atctcccact
     6061 gtaccaacat tcgtatcata tccatctggc aacttttcaa taaattcatg acattgtgct
     6121 aacttggcag ctttttcaac tgcctcatcc gtagcttctg gattgccaag tttaatattt
     6181 tctttaaaag taagatttaa taaaaagtta tcttgtccaa caaaaccaac taaatcgtta
     6241 agttgtttcg attcaatatc ttttatatct ataccgccaa tcgtaatttc acctgaagtc
     6301 acatcccagt atcgtgatat aagcttggca atggttgact taccgctacc agatgccccg
     6361 acgatagcta tgaaattatt ttctggtact gtaaatgata aatgcttaaa gaccagatcg
     6421 tccttatctt tgttatatga aaatccaaca ttattaaaag caatttcata atgttgaggc
     6481 ttcttaaatt ttgttgacaa tactaactct tctagactta atatttgatt cacttcagtt
     6541 aatgcgtatt gtatagactt taaatgattt acataattag taaaattctt aatcggtgct
     6601 actacaccta aagataatac gatgcatagg aaaaattccg catagtttaa ttggttaata
     6661 gatatcaaat acatgccgac cggtaaaatc cctaaaaatg ttgaaggtaa tacactagct
     6721 cctaaattca tataccccca tgtattttta aaccaattca aagtgtgaat cttataatta
     6781 tctactgcat ctttatattt tttatatgaa ctttgagatt gattaaatgt tttaatcact
     6841 tcaatgcctt cgataaactc tacaatcgcg ctattcatat aattattcga tttcatttgt
     6901 tcagcatatg tctcattaaa tccagacata acttttttaa aagcgaaaat tgaaattggt
     6961 atcgttacta ataaggcact agccatacgc caatcaatga gcattatgta taaaaagata
     7021 gcagctgaca aaagtaagtt tcctataact tcaggaatca tatgtgctaa aggtaattct
     7081 attgtttcaa ccttatcgac aaatatattt tttaattcac ctattttctt agattccact
     7141 acgcctaaag ggagacgcat taatttttga gctaattttt tacgaatttc agataaaatt
     7201 tcatatgccg taatatgtga tagcatcgtt gacgctccaa aacaacacac ttgtgaaata
     7261 taagcgatta aaacaataaa gatataaacc ataatcgaat taatcgtata tgcattgtta
     7321 atcatcatta aaataatttt aaagactgcc caatatggaa ctaatccaga aaagacactg
     7381 atgatagaca acaaaattga taacataatt ttccatttac tatcagaagc aaatccgctg
     7441 agtatcttta tccaatttgt ctgattgttc atcgtatatg taactccttt caattaataa
     7501 tctaaattaa gccgcttata ttatttattt cactggatga tatacataat ataaatttgt
     7561 tatttgttaa aaattaatac ttattacaag tacatcatat attagttgat aacgattatc
     7621 aatgtcgcgt ggatttgtga cacatttctt ttaaaaattc acaaggttat ggggcagaaa
     7681 tgataaagag ccactaatga tttattatgt aatgattctt tatccttagc cacagctaat
     7741 atgt
//
LOCUS       DAMZPP010000131         7721 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMZPP010000131 DAMZPP010000000
VERSION     DAMZPP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.131"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(10..1551)
                     /gene="guaA"
                     /locus_tag="POH73_002257"
     CDS             complement(10..1551)
                     /gene="guaA"
                     /locus_tag="POH73_002257"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDI6888181.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCKGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(1576..3042)
                     /gene="guaB"
                     /locus_tag="POH73_002258"
     CDS             complement(1576..3042)
                     /gene="guaB"
                     /locus_tag="POH73_002258"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDI6888182.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(3080..4348)
                     /locus_tag="POH73_002259"
     CDS             complement(3080..4348)
                     /locus_tag="POH73_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDI6888183.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILVLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFKFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(4348..4926)
                     /gene="xpt"
                     /locus_tag="POH73_002260"
     CDS             complement(4348..4926)
                     /gene="xpt"
                     /locus_tag="POH73_002260"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDI6888184.1"
                     /translation="MELLGQKVKEDGVVIDERILKVDGFLNHQIDAKLMDEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(5157..5259)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5439..5846
                     /locus_tag="POH73_002261"
     CDS             5439..5846
                     /locus_tag="POH73_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDI6888185.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDKMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVGV
                     RDHSSHQEVLENNSAYEEVDITHFAEASKGPKA"
     gene            5989..6651
                     /locus_tag="POH73_002262"
     CDS             5989..6651
                     /locus_tag="POH73_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888186.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYDLLEEMDLRK
                     PF"
     gene            6769..>7721
                     /locus_tag="POH73_002263"
     CDS             6769..>7721
                     /locus_tag="POH73_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888187.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTTKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKVLAESSNKVENNTATDEKAESKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQPKNDKAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
BASE COUNT         2568 a         1502 c         1227 g         2424 t
ORIGIN      
        1 tatatataat tattcccact caattgtgct tggtggtttt gatgtaatgt catagactac
       61 gcggttgacg tgatctactt cgtttacgat acgactagaa atcttttgta agacttccca
      121 atcgatgcgt gcgaagtcac ttgtcatgcc gtcgatagat gttactgcac ggatacctac
      181 tgtgtgatca tacgtacggt agtctcccat aacacctact gattgaatgt ttggtaacac
      241 tgtgaagtat tgccaaattt ctctttcaag accttcttct ctaatcactt ggcgtaaaat
      301 cgcgtctgat tctcttacga tttctagttt atcttcagta atttctccaa gtacacgaat
      361 accaagacca ggtcctggga atggttgtcg ccatactaaa tgttctggaa tacctaactc
      421 aatacctaat ttacgtactt catctttaaa caatgtattg attggttcga ttaattcgaa
      481 ttccatgtct tctggtaatc caccaacatt gtgatgtgat ttgattgttt gtgctgtctt
      541 agtacctgat tcgatgacgt ctgtatatag tgttccttgc gcaaggaagt ctacaccttt
      601 aagttttgat gcttcatcat caaacacata tacaaattca ttaccaatga ttttacgttt
      661 ttgttcagga tctgaaacac cttttaattt attcataaag cgatctttcg cattaacacg
      721 gataatattc atgttgaaac cttcaccgaa ttgctccata accatatcgc cttcaccttt
      781 acgaagtaag ccatggtcta caaagataca tgttagttga tcacctattg ctttatgcaa
      841 tagtacagct acaacagatg aatctacgcc gccactcatc gcacataata cacgacggtc
      901 tcctacgcgt tgacgaatct tttcaatttc gatttcgata aagttttcca ttgtccattg
      961 acctttacaa tcacaaacac gacggacaaa attatttaat aaatcattac catattctgt
     1021 atgacgtact tctggatgga attgaacacc ataaatgcga cgtttcttat cttcgattgc
     1081 tgcatagtct gtacttggac tatcagcgat aacttcaaag ccttctggaa tttcaataac
     1141 tttatcagaa tgactcatcc aaacagtttg ttctgctggt aagccagcga ataactcatc
     1201 tgacttcgca ttaatgattg ctttaccgta ttcacgttca ttggcacgtt caactttacc
     1261 acctaataat ttagtagtta attgcatgcc gtaacaaata ccaagtactg gaattcctaa
     1321 attatatatt tccggatcaa ttgtaaatga accttcttca taaactgaat ttggaccacc
     1381 tgataagatg ataccttttg gattcatttt cttaatttct tcaattgaaa tttcatgatc
     1441 gtgtaattca ctataaacgc ccatttcacg aattcggcgt gtaattaatt ggttgtattg
     1501 gctaccaaag tctaagacaa ggattaactc ttgttctttt gccatttcca tatttgtcgt
     1561 tctcctttat cttaattaga atgagtagtt cggtgattct ttcgtaattt gaatattatg
     1621 tgggtgactt tctgctaaac cagcagggcc catacgtgtg aattgtgctt cttcgcgtaa
     1681 ttctcttaaa tcgtgtgaac cagtataacc cataccagca cgcacaccgc ccattaattg
     1741 gtaaattgta tcttgtaacg cacctttata tgctgtacgt ccttcgatac cttcaggtac
     1801 aaatttctta ggcgctttgt cctcttggaa gtaacggtcg tttgaacctt tttccatcgc
     1861 acctaaagag cccataccgc ggtatacttt atattgtcta ccttggaaaa tttctgttgc
     1921 gcctgggctt tcttcagtac ctgctaataa gctacctaac ataaccgcat gtccaccagc
     1981 agctaatgct ttaatgatat ctcctgagaa tttaatacca ccatcagcaa tgatagcttt
     2041 accatgtttg cgtgcttcag ttgcacaatc ataaattgct gtaatttgtg gtacaccaac
     2101 acctgctaca acacgtgtcg tacaaattga acctgggcca ataccaactt taacaatatc
     2161 tgcacccgct tcaaataaat cctttgttgc ttctgcagtt gctacgttac ctgctactaa
     2221 tgtgatttct gggtaagtct tcttaatatg tttcacttga tcgataacgc ctttagagtg
     2281 accatgtgct gtatcgataa ctaagacatc cacacctgct tcgactaatt tttgagcacg
     2341 aatatcagtg tcttttgaaa taccgattgc tgcggctaca agtagacgac catgttcatc
     2401 ttttgctgca ttagggaatt cgataacttt ttcaatatct ttaatagtaa taagaccttc
     2461 tagacgcccg tctttaacta atggtaactt ttcaatctta tgtttttgga gaattttttc
     2521 tgcttcttca agtgttgtat tcactggagc tgtaattaaa ttttcttgcg tcattacatc
     2581 tacaatttta attgagaagt cttcaataaa acgtaagtcg cggtttgtta aaatacctac
     2641 taagttgcga tcttctttat tatcaacaat tggtacacct gaaatacggt atttacccat
     2701 taatgcttcc gcttcataaa cactttcttc tggtgttaag aaaaatgggt ttgaaatgac
     2761 accattttct gagcgtttta ctttttgaac ttcgtccgct tgttcttcaa cgcccatatt
     2821 tttatgaata acacctaaac caccttgacg agccatagca atcgccattt tagattcagt
     2881 tacagtatcc ataccagcag aaataactgg aatatttaat ttaactttgt ctgataattg
     2941 tacgcttaaa tcaacgtctt tcggtaaaat atcagattgt gctggaatta ataacacatc
     3001 atcaaacgtt aatgattctt ttgcaaattt actttcccac attaaaaaca gcctccattt
     3061 ttcaaattaa ttagttatat tatttcacat tttcttccgt ttgtttatac tttattccat
     3121 taaaaaagaa attaagaata attgcagaga ttgcgcctaa aacaattccg ttttgggtca
     3181 accatgcaaa ttgttcacct aaacctttaa atgcttgtgg tacagcgctt ataccagtac
     3241 ctaatcctac tgatacagcg ataattaata aattgttttg atttttaaaa tcgatatgtc
     3301 ctaatatact aacaccatat gccattacca tgccaaacat agctatcatc gcaccaccta
     3361 acacaggtag cggtatgata tttgctaatg cgccaagctt aggtatacaa ccacatataa
     3421 gtaataacac gaccatgccg tatataacat tgtttttctt agcgccggat aaagaaacaa
     3481 gtcctacatt ttgcgaatag gctgtatacg gaaatgaatt gaatatagaa cctaacacta
     3541 tcgctagacc ttccgcagta taacctttac gaaaatcttt tctttctaac ttcttaccgg
     3601 taatttcact taacgcatga tagacacctg tcgactcaat taaactaacg atagctacaa
     3661 taaagaacac taacgtcgat gtcacatcaa agctaaatcc tgagaattta aacggcactg
     3721 ggatgcctaa ccaaccggca tgattgactt gattaatatc gaccatccca agtaagccag
     3781 cacctatcgt tcctaaaacg agtccaatta atatggcaat actcttaata aatccagttg
     3841 tgaatctttg taaaacaaga ataatgatta atgtcattaa acctaacaaa atgttcttaa
     3901 catctccata gtcctttgca ccttgacctc cagctaagta attcattgct actggcatta
     3961 aattgatacc aatgatagta acaacactac ccgttactac tggtgggaag aatttcacaa
     4021 gatgtgaaaa gaaaggcgcg atgataataa ctaatatccc tgataaaaat agcgaaccgt
     4081 aaagtacatc tattcctttc gtttgaccaa ttaaaatcat gggcgcaaca gccgtgaatg
     4141 tacatccaag aacgattggt aatcctgttc ctgttacttt attggcttgt aaaaatgtgg
     4201 caactccaca cataaatata tctactgtaa ctaagtaagc gatttgttca ggcgtaaact
     4261 tcaaacttgt accaacaatg attggaacta agatggcacc tgcgtacata gctaaaagat
     4321 gttgaacact taggattaaa tttttcatta ttcttctccc accaatgtca ctttatttcc
     4381 ttctagtgaa gcaaccttgc agagagaaga aactgttaag cctgcttctt ctaaacgttg
     4441 atgcccattt tggaaactct tttcaacaac tataccaata ccagctgtct tagcattcgc
     4501 ttgctgtgcg atatcgtata atcctaatga agcatcacca tttgctaaaa agtcatcgat
     4561 gatgagtaca gtatcttctt ctgataaaaa ttcttttgaa acaatgaccg tacttgtttt
     4621 atttttagta aatgaatgaa tagatgtttc ataataacca tccgtcaaag tgctaggttt
     4681 tgcttttttc gcaaataaac atggcacatc aaaatgcagt gcagccatga ttgctggtgc
     4741 gataccggaa gcttcaatgg ttaagatttt agtaatccct ttatctttaa attgctcgta
     4801 aaaagtgcga ccaacttcat ccattaactt tgcatcaatt tgatgattta aaaatccatc
     4861 gacttttaaa atcctctcat caatgacaac gccgtcttcc tttacttttt gtcctagtaa
     4921 ctccactctt aaaacctcct cagatttgtg tgaaacattt tgctcttaaa ttggtgctag
     4981 atacaaaaaa atccccaaac taaataatag tttcagggtt tatgagtgaa cgaacatgca
     5041 tcacgtattg gtcatgcaat caatgtaaaa gaagtttcat caaataaact gtgaccatca
     5101 tataaaatga tataaatcac ccatcatggt tacaatttaa tggctgaagc tactcctagt
     5161 attgtgttgt tactcatagt catgtcgttc aaggcaacat ggtagaaact tctaaagcca
     5221 tattctttag attatatgag tttatgtaaa ttatttaacg ataatagcaa attttcggca
     5281 ttttttcaat aactgcttag gtaaactttt aatagtttta taaatgtaca gagacgttta
     5341 atgcgaatga agacttataa cttaataatg taagggtttt actttataat aagaagggta
     5401 aaagattgaa taaacaaatt tttaaagggg cgacaactat ggcagatatt actgtagtaa
     5461 atgacactgg cgaattatac aacgtaatca atcaaaagaa atctgaaggt tatttagaat
     5521 cagagctaac aattattagt aaaagtaaat tacacctaaa tgacttacac gactctgaaa
     5581 tctcccttat ctcaacaagt ggaacattta gtgataaaat gactaaactg ttaacaggag
     5641 aagatggtga acatgcagtt ttatcacgct acaacttagc gcctgatgaa ttagaaaaat
     5701 ataaacaact tattttagac gataaaatgt tagttgttgg cgttcgcgat cattcatctc
     5761 accaagaagt actagaaaat aattcagctt atgaagaagt tgatattaca cattttgctg
     5821 aagcatctaa aggtcccaaa gcttagttat cacactgaat tcaaaatgag attactgtac
     5881 tgtctaattt aagatagtac agttttttat accatttaaa agtttatgaa cagtctttta
     5941 caacaggcga ttatgctata atattattta aattggggag gtgttcttat ggaatttaaa
     6001 accattcaat ctgtcaaaga tccacattac aacgaagcac ttaaacttta cgatactaag
     6061 ttaaatttat ctttaactga agataaacgt atcatcaaac gctcattaga aaacaacaaa
     6121 actgaaaacg attatgtctt tatcgtaggc ttagaaaacg atgttgttgt cagtctagca
     6181 actgcacatt atgaagccac aaccaattct gcatttttaa tttatttatt tgcaaaagac
     6241 ctacccaacc atgatgagtt aatgacacaa acattacatc aaattgaaca tgaattaaac
     6301 ttattatcaa accttgttca ttctagaagt attaactttt taatgatgga agtccctaaa
     6361 gaacctgcag atttaacaca agataatggg ctcattattg aacaccgtag acagttttta
     6421 tacgaacatc aatttgaaag acaaactgaa attgattaca ttcatccaac taatgatggt
     6481 cacactaaaa aagtggactt atttttaaag aaaaatatta aactgacaaa ggatatctac
     6541 ggtacaagtg ttaaatcaaa ttatattttg aagtatgtat ttgcaaatgg tatcgcacgt
     6601 gaaattattt atgatttatt agaagaaatg gatttacgta aaccatttta aacaaacttg
     6661 aaatcactaa caataacgtt taaactatgt atgaagcaag actgacgttt cactgcccgt
     6721 agacaaacgt cagtatacat aaagtatatg cagaaaggac tttaaagtat gttaactaaa
     6781 gaatttgcac aacgcgtaga actaagtgag aagcaagtcc gtaaaattgt tcaacattta
     6841 gaagaacgtg gttaccaact aagcaagact gaatatcgtg gtcgtgaagc aactgatttc
     6901 aaagaagaag atatcgagct tttcaaagac atagccgaca aagtaaaaca aacaaatagt
     6961 tatgatctag cgtttgatga attagaaaaa gaaaaagact tcctgcaagt cattgtcaaa
     7021 aacgatgaca aaaacttacc tactaatcaa aatgttgctc aactagtaga agatttgcgt
     7081 ctagaaatcc agaaaatgcg cgaagaacgt cacctacttg gtcaaatgat gaatcaagta
     7141 catcagcaac aacaagaatt aaaagaactt caaaatcaac ttacaactaa aatcgattca
     7201 aatagcgaat ccttaaaagc catccaaaca tcacaagagg ctatccaaga agcacaagca
     7261 tctcaagcta aagtattggc tgaatcatca aataaagttg aaaataatac tgctactgat
     7321 gaaaaggctg aaagtaaaga ttcgaaggtt gctggagtga atacatcaac agacgctaag
     7381 acggatacta aagcagaaaa tgcaggcgat ggcactgcta ctaaagtaga taaagaggat
     7441 caaatttctg caactgaagc aattgaaaaa gccagcgttg agcagcctaa aaatgacaaa
     7501 gcagctgaga cttcaaataa agaagctaca gtagatgcag aagcgcaaca tgatgctgaa
     7561 caacaagtag cagaagccca tgcagaagct tctaaacaag ctacttcgaa cgattcattg
     7621 gaagcgaaag cagaaaatga cagcacagct tcacagtctg aaatgtcaga accaaaacct
     7681 caagaagaga aaaaaggttt cttcgcacgt ttattcaacc t
//
LOCUS       DAMZPP010000132         7710 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMZPP010000132 DAMZPP010000000
VERSION     DAMZPP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.132"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..468
                     /locus_tag="POH73_002264"
     CDS             <1..468
                     /locus_tag="POH73_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6888188.1"
                     /translation="TTQYFQTDIFSSVTVIILATLASLLPDICHTQSKIGRRFKVISF
                     FVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGLVSHVILDMITPKGVKL
                     FYPLPFNVKTPIQFKTGGLVDLSLATALMIGTVYVLFQPFINDIISNWNTKFL"
     gene            512..1699
                     /locus_tag="POH73_002265"
     CDS             512..1699
                     /locus_tag="POH73_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HDI6888189.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQAKQLKELHRQTGHKIQWYIMTSDINHEETLAYF
                     ESHNYFGYDQESIHFFKQDNIVALSEAGQLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVKVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            1718..2401
                     /locus_tag="POH73_002266"
     CDS             1718..2401
                     /locus_tag="POH73_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDI6888190.1"
                     /translation="MSKSSEKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAYGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            2579..3922
                     /locus_tag="POH73_002267"
     CDS             2579..3922
                     /locus_tag="POH73_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6888191.1"
                     /translation="MRLKSIVTVIALILIMFMAAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLTRFKIIYIAIAGLTLFTVGSLMSGLSTHFSMLVISRV
                     IQGFGSGVMMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFYINIPIAIIAIILVVWTFHFPEEETVAKSKFDTKGITLFYIFIGLIMFALLNQQH
                     LYLNIIGFVLAILVALRLFNIEKKVSSPFLPVAEFNRMITLVFITDLLTAVCLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHHIEAKLSRKVIYLSSFTLLLLS
                     SIIIAFGIKLPILIACVLILSGLSFGYIYTKDSVIVQEETSPIQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYALKSGLFGANLHNILGVVSLISVGLIVVWLIFYREAASQTKE"
     gene            4044..4517
                     /gene="sepA"
                     /locus_tag="POH73_002268"
     CDS             4044..4517
                     /gene="sepA"
                     /locus_tag="POH73_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HDI6888192.1"
                     /translation="MVMNYLKHKFYNLLTTIIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIVFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYMVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            4706..6149
                     /gene="lmrS"
                     /locus_tag="POH73_002269"
                     /pseudo
     CDS             4706..6149
                     /gene="lmrS"
                     /locus_tag="POH73_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
     gene            6297..7361
                     /locus_tag="POH73_002270"
     CDS             6297..7361
                     /locus_tag="POH73_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HDI6888193.1"
                     /translation="MLTVDQVKELIGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     VAMAQLGGAPQLDLQMAVVNVLKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGINGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVRWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            7450..7636
                     /locus_tag="POH73_002271"
                     /pseudo
     CDS             7450..7636
                     /locus_tag="POH73_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2580 a         1008 c         1417 g         2705 t
ORIGIN      
        1 acaacacaat attttcaaac ggatattttt tcgtcggtta cggttatcat tctagcaact
       61 ctagctagct tattacccga tatatgtcac actcaaagta agattggcag aaggtttaag
      121 gtaattagtt tttttgtgag attgatcttt ggacatcgaa cctttacgca ttcaatatta
      181 tttatcgcaa tcatagcatt tttattgcaa attattcaga cgcctaatta ttatatggca
      241 gcaattataa ttggattagt gtcacatgtt atactagata tgataacacc aaaaggcgtg
      301 aaattatttt atccgttacc gtttaatgtg aagacaccga ttcaatttaa aacaggtgga
      361 cttgtagact tatctttagc tactgcgtta atgatcggaa cagtttatgt tttatttcag
      421 cctttcatca atgatataat aagcaactgg aatactaagt ttttatagaa aataaattat
      481 taaaggttac aaactcagca ggaggcataa aatgctagat aaaaatcaat tagctaaata
      541 taaacaagat catttatgtg aatatgaaaa aataatgagt aacaatgaaa aagaagcact
      601 tgaagagaag gtagcgtcat tagatttaga ttttattgcc aaattatata acgatttata
      661 catcaataag aaaacgatag atgatgtatc tgcagtttct gaagtgaaat atgatattaa
      721 atcacaaatg agtgatgatg agattaagcg tttagaggaa caaggacttc aagctattaa
      781 agaaggacaa tttgcagtac ttttgatggc aggtggacaa ggaacaagac ttggatataa
      841 aggacctaaa ggttcattcg aaattgaagg tgtaagttta tttgaacttc aggcaaaaca
      901 attaaaagaa ctacatcgac aaactggtca taaaattcaa tggtacatta tgacaagtga
      961 tatcaatcat gaagaaactt tagcttattt tgaatcacat aactactttg gttatgatca
     1021 agaatcaatc catttcttta agcaagataa tattgtggcg cttagtgaag caggacaatt
     1081 aattttaaat caacagggtc gtattatgga aacgccgaat ggtaatggtg gcgtgtttaa
     1141 atctttagac aaggcaggat acttagaaga aatgtctaat aatggcgtta agtatatttt
     1201 cttgaataat atcgataatg ttttagtaaa agtgttggat ccactatttg caggatttac
     1261 ggtagaacat gattacgata ttacttctaa aacgattcaa ccaaaaccag gagaaagtgt
     1321 tgggcgatta gttaatgtcg attgtaaaga tacagtattg gagtattcag aattagatcc
     1381 agaagttgca aatcaattta acaatgctaa tatcggtatt catgcattta aacttggttt
     1441 tattttaaat gctgtaaatc gtgaattacc ttatcattta gctattaaga atttgaagca
     1501 attagatgag aactttggtg ttattgaaca accaacttta aaattcgaat tgttctattt
     1561 tgacatattt acttatggaa ctagttttgt cacgttacaa gttcctagag aagaagagtt
     1621 ctcacctctt aaaaataaag aaggtaaaga tagtgttgca acagcgactg aagatttacg
     1681 tagaatgggt ttaatttaat gttaggcggt gacttacgtg tccaaatcta gtgaaaaaag
     1741 taatgacaca atcgtagaaa catttaagga tatcatccct ttatcatttg gagaagaaat
     1801 tggtaatgca gcatctcatg gtgttgcagc tttgttaaca ttattagtgt taccctatgc
     1861 agctgtgcac agttatattg catatggtac gcttgcgtct gtgagtatgt ctatttatgt
     1921 tatttctatc tttatgatgt ttatttcatc tactatttat cattctatgc aaaatgaaac
     1981 gcctcataaa tatattttaa gaattattga tcatagtatg atttatgtag caatttcagg
     2041 tacatatacg cctattttat taacagttgt aggtggttgg attggatgga cagtgtttat
     2101 cctattatgg ggcacaacgc tttggggaat tttatataaa tccatagctg tcaaagttaa
     2161 ccaaaagtta agcttaattg tctatttagt gatgggctgg gtaggtatta tcttgttacc
     2221 aattattttt attcgcacat catggatatt catattattc attttcttag gcggtgtgtc
     2281 atatacgatt ggtgcatggt tctatgcaca aaaaaacaga ccatattttc atatgatatg
     2341 gcatattttt attgttatag catcattatt gcatttaatt gctattttat attttatgta
     2401 atagtttatc agaaggtggg gggaactccg ctttttttat tacttaattc attctaatag
     2461 aggattgttt tgtatgacta ttatcgttaa gatttgaaag tcaaaagtgt ttatacaaat
     2521 acaaatcaat agtttgtaaa taaaatcgta taaatatata taaagaagga ggtatagcat
     2581 gcgtttaaaa tcaattgtaa ctgtaatagc tttaatatta atcatgttta tggctgcaat
     2641 cgaatcatca attatttctt tagcgttacc aacaattaag caagatttaa atgcagggaa
     2701 tttaatttca ttaattttta ccgcgtattt tatcgcatta gtaattgcca atcctatagt
     2761 aggtgaatta ttaactcgtt ttaaaattat ttatattgct atcgcaggtc ttacactatt
     2821 cacggttggt agtttaatgt caggtttaag cacacatttt agtatgttgg ttatatcacg
     2881 tgtaattcaa gggtttggat ctggtgtcat gatgtccttg tcacaaattg tgcccaaatt
     2941 agcatttgaa attccattac gctataaaat tatgggtatc gttggtagcg tttgggggat
     3001 ttcgagtatc attggaccac ttctaggtgg tggcatatta gaatttgcaa catggcattg
     3061 gttattctac ataaacatac cgattgcgat tattgcaata atattggttg tctggacatt
     3121 tcattttcca gaagaagaaa ctgtggctaa atcaaaattc gatactaaag gtatcaccct
     3181 gttttatatt ttcattggtt taattatgtt tgcattgtta aaccaacaac atttatattt
     3241 aaatatcata ggttttgtct tagcaatatt agttgcactt cgattattta atattgagaa
     3301 aaaagtttca tccccattct taccagtagc agaatttaat cgaatgatta ctttggtctt
     3361 tattacagat cttcttacag ctgtttgttt aatgggcttt aatttatata ttcctgtgta
     3421 tcttcaagaa caactggggt tatcgccatt gcaaagtgga ttagttattt ttccactttc
     3481 tgtagcgtgg attacattga attttaattt gcaccatatt gaagcgaagt tatcaagaaa
     3541 agttatctat ttgtcgtctt ttacattatt attattaagt agtataatca ttgcgtttgg
     3601 tataaaatta ccaattctta tagcatgtgt cttaatttta agtggactaa gctttggata
     3661 tatttataca aaagatagtg taattgtcca agaggaaaca agtccaatac aaatgaagaa
     3721 aatgatgtcg ttttatggat taacaaaaaa ccttggcgca tcaataggtt cgacgattat
     3781 gggatacctt tatgcgttaa aatcaggatt gtttggtgca aacttgcata atattttagg
     3841 cgttgtatcc ttgataagtg tagggctcat cgttgtatgg ctcatatttt atagagaagc
     3901 ggcgtcacaa acaaaagaat agaattatga ttaaaattga tgtagtgtaa ataaagtaac
     3961 aaataatcat tttactaagt ttgaagtgta atcaactatc ttaaaattaa aagcaatata
     4021 aattatttat ggtaaggagg acaatagtta tgaattatct taaacataag ttttataatt
     4081 tattaacaac aattattgtt ctctttatct ttgtgctttc tggtgcgata ttcttaacgt
     4141 ttttagggtt cggactatat ggattaagta gaattcttat ttattttagg ttaggtgact
     4201 tcacatataa cagaagtatg tacgacaatt tgctatatta tggtagttat attatatttg
     4261 gctactttat cgtatttgca gttgaacatt tgatggatta ttttagaaaa atgttacctg
     4321 aaaatgctta ttttagaggt gcaacttttc atttaatctc ttatatggtt gcaacaacgt
     4381 tattttattt tattattcat ctgaactatg tgtatatcaa cattgatttt tgggtcatca
     4441 tggttattat aggcttttta tatgtttgta agctgcagtt ttaccctgaa agtaaaaatc
     4501 taaataatag aaaatagagt tttgaagttg ggggcaatga tgtgttccaa cttcttttta
     4561 ttcatatagt agaaaatgag ttgttttgct tcaattaatc acggttaata ataaccattg
     4621 atataaatag ttggtacttt aatctgaaat atattttaag tatatatgct aataaacgta
     4681 aatttgttac aaaggaggaa ttatgatggc taaagttgaa ttaacaaccc ggagacgaaa
     4741 cttcatagtt gctgttatgt tgattagtgc gtttgtagct attttaaatc aaacattatt
     4801 aaatacagcg ttacctagta taatgagaga attaaatatt aatgaaagta catcgcaatg
     4861 gctagttact gggtttatgc ttgttaatgg cgtcatgata cctctaacag catacttaat
     4921 ggacaggatt aaaactagac ctttatactt agcagcgatg gggacatttt tagtaggatc
     4981 aattgttgca gccatggcac ccaattttgg agttttaatg ttagcacgtg tcatccaagc
     5041 tatggggggc aggcgtactc atgcctttaa tgcaattcac attgtttacg ttattcagta
     5101 aagaacatag aggatttgca atgggattag ctggtttagt tattcaattt gcaccagcaa
     5161 tcgggcctac tgttactgga ttaatcattg atcaagcgag ttggcgagta ccatttatta
     5221 taattgtagg aattgcttta gtcgcattca tcttcggttt aatttcaatt tcaagctata
     5281 atgaagtgaa atatactaag ctagataagc gctctgtaat gtattcaacg attggatttg
     5341 gattaatgtt atatgcattc agtagtgcag gcgacctagg atttactagc ccggtagtca
     5401 ttggtgcaat gataattagt tctattatca tctacttatt tattcgtaga caattcaata
     5461 taagcaatgc acttttaaat ttaagtgttt ttaagaatag aacatttgca ttatgtacga
     5521 ttagttcaat gattataatg atgtcaatgg ttggtccagc gttattaata ccgctctatg
     5581 ttcaaaatag cttagctcta tctgcattat tgtttggact tgttatcatg ccaggtgcaa
     5641 tcataaatgg tattatgtcg gtttttacag gtaaatttta tgataagtat ggtcctagac
     5701 cactgattta tactggtttt acgattttaa caattactac tattatgttg tgtttcttgc
     5761 atacagacac atcttatacg tatttaatag tcgtatatgc aattagaatg ttttcagttt
     5821 ctttactcat gatgccgata aatactacag gaattaattc tttgaaaaat gaagaaatct
     5881 cacatggcac ggctattatg aactttggtc gtgtaatggc tggctcatta ggaacagcat
     5941 taatggttac tttgatgagt tttggcgcaa aaatattctc atctacatcg ccatcgcatt
     6001 taactgcaac tgaaattaaa cagcaatcaa ttgctatagg ggtggatatc tcatttgctt
     6061 ttgtagctgt gcttgttatg gcagcttatg tgatagcgct ttttataaga gaacctaaag
     6121 aaatagaaag taatagaagg aaattttaaa ataattatag tagttggtct attgaaaata
     6181 ataggctaac ttcttttttt atttaatgaa aagttttata cttttattga tagactaagc
     6241 aaaaattgtt atttgctatg atgtagatat tttaaattga ttaaggggga tttgctttgt
     6301 taacggtaga tcaagttaaa gaactaatag gagaaattaa agatcctatt atagatgtac
     6361 ctttaaaaga aacagaaggt attgtagaag tttctattaa ggaagaaaaa gaacatgtaa
     6421 gtgttaaagt ggcaatggca caattaggtg gtgcaccgca attagattta cagatggcag
     6481 ttgttaatgt tttaaaagaa aatggtgcaa aaactgtggg tatacgattt gaaacgttac
     6541 cagaagaaaa agtaaatcaa tttaaaccaa aagaagaaaa taaacctaaa acgatagaag
     6601 gcttattatc tcaaaataat ccagttgaat ttattgctat tgcttcaggt aaaggtggcg
     6661 tcggtaaatc tactgttgca gtaaatttag ccgttgcctt agcccgtgaa ggtaaaaaag
     6721 ttggattagt agatgccgat atatatggat ttagtgtacc agatatgatg ggtattgatg
     6781 aaaagcctgg aattaatggg aaggaagtaa ttccagttga acgtcatggc gttaaagtta
     6841 tatcaatggc attttttgtg gaagaaaatg caccagttat atggagagga ccaatgttag
     6901 gtaaaatgtt gacgaatttc tttacagaag ttagatgggg agacattgaa tatttaatac
     6961 tcgatcttcc acctggaaca ggagatgtag ctttagatgt tcatacgatg ttaccttcaa
     7021 gtaaggaaat tattgtaacg acacctcatc ctacagcagc atttgttgca gctcgcgcag
     7081 gtgcgatggc aaaacatacg gatcattcta ttcttggcgt aattgaaaac atgagttatt
     7141 ttgaaagtaa agagacgggg aataaagaat atgtctttgg caaaggtggc ggtactaagt
     7201 tagctgatga acttaatact caattacttg gggaattacc tttagagcaa ccatcttgga
     7261 atccaaaaga ttttgcaccg tcaatatatc aatcagatga tcgtctaggt aaaatttata
     7321 gctcgattgc acaaaaagtt atagctgcca caaataaata ggtataatat atgctaaacg
     7381 tacaaaatta cttttatttt gtacgttttt atattgattt gttttggaat gctggtaaaa
     7441 taagttcttg tgttaagcaa taaacgcatt gaaaaaagaa gcgacttgca aacacgaatg
     7501 tttttcaaaa aagttattga ctttaaatta taaagttgat aagataataa agtcgtcaaa
     7561 aacggtgatg atgtttttaa agaagaattt caaaagtgta aaacttacca ttgaaataac
     7621 tataaaaaca atataaaata aataaagtaa tcgttcgaaa agttattgac ttcaataatt
     7681 aaatgaagta caataaaaga gattgaacat
//
LOCUS       DAMZPP010000133         7629 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMZPP010000133 DAMZPP010000000
VERSION     DAMZPP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.133"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..5039)
                     /gene="ebh"
                     /locus_tag="POH73_002272"
     CDS             complement(<1..5039)
                     /gene="ebh"
                     /locus_tag="POH73_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HDI6888194.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGLNTSQAQAAETNQ
                     PASALKQKQQNGDTQTENREVEVQNSQNGQSLSAPIENEQPNNNQTNHVDASAVQSST
                     TEHDQPVSQNEQAKKDTAAATPTQSAKAASKHEQSESRAANKKENDNKATHVESHEAN
                     VVTASDSSDSGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKINTLL
                     GSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSSRIQTRSIESRAAEPRSVSD
                     YQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDAKEIALITAK
                     QTGDGYQWVIKFNKGHAPHENMIYWFALPAGQTPVGRTEFVTVNADGTNVQWSNGAGA
                     GANKPLPEMWPYGVNDSRSWDYKIRNRSGQVIYDWPTVHINSLKDLARASDYFSEAGA
                     TPATKAFGRQTFEYINGERPTESPGVPKVYTFIGKGDASYTISFKTQGPTIDKLYYAA
                     GGRALEYNQLFMYSQLYVESTQDYQQRLNGLRQVVNRTYRIGTTKRVEVSQGNVQTKK
                     VLESTNLNIDDFMDDPLSYVKTPSNKVLGFYPTSANTNAFRPGGVNPLNEYQLSQLFT
                     DDKLQQAARTGSPIRLMIGFDYPDAFGNGETLVPVNLTVLPEIQHNIKFFKNDDGQNI
                     ADKPASKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQITGI
                     PRTLHIENSTNRTNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTPRITT
                     TAEELRGTALQKVPVTVTDIPLDPSALVYLVIPTSQTRDGGSEADQIPSGYTKIATGT
                     PDGVHSTITIRPEDYVVFIPPVGNQIRALIFYNNVVASNMSNAVTILPDDIPPTINNP
                     VGLNAKYYRGDEVSFTMGVSDRHSGLKSTTITTLPSGWTSNLTKSDKKNGSLAISGRV
                     SMNQAYNSDITFKVSATDNVNNTTNDSQSKHVTVHVGKISDDAHPIVLGNSEKVVVVN
                     PTALTGDEKQRITTAFMNKNQNIRGYLASSNPVTVDNHGNVTLQYRDGSSTTLDATNV
                     MTYEPVVKPEYQTANAAKTATVTIAKGQSFNIGDIKQYFTLSNGQAIPSSSFTNITSD
                     RTIPTAQEVSQMNAGTQLYHIVATNAYHKDTEDFYITLKIIDVKQPEGDQRVYRMSTY
                     DITTDEISKVKQAFINANRDAISFAEGDISVTNTPNGSNVSTITVNINKGRLTKSFTS
                     NLNNMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGTQITT
                     NDILTLLKATTTVPGLRNNIAGNEKAQAEAGGRPNYKTTGYSQSNPTSDGQRQFTLNG
                     QVIQIMDIINPSNGFGGQPVTNSNVRANHSNSTVVSVNESAANGAGAFTIDHVVKNNS
                     THNAADAVYKAQLYLSPYGPKQYVEHLNQNTDNTNEAINIYFVPSDLVNPTISVGNYT
                     NHQVFSGETFTNTITANDNFGVQSVTVPTTSQLTGTVDNNHQHVSATAPNVTSTTNKT
                     INLVATDTSGNTATTSFNVTIKPLRDKYRVGTSSTAANPVRIANISNNATVSQADQTA
                     IINSLTFTSNAPNRNYATASANEITSKTVSNVSRTGNNAQVTVTVTYQDGTTSTVTVP
                     VKRVIPEIVAHS"
     gene            complement(5440..6831)
                     /gene="norB"
                     /locus_tag="POH73_002273"
     CDS             complement(5440..6831)
                     /gene="norB"
                     /locus_tag="POH73_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HDI6888195.1"
                     /translation="MEKPSRETFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITILAIAIVSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTFGCKKP
                     MLIGTAVLIVGECLISLTFLPEILYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIDTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
     gene            complement(6988..>7629)
                     /locus_tag="POH73_002274"
     CDS             complement(6988..>7629)
                     /locus_tag="POH73_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6888196.1"
                     /translation="DLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTS
                     KILFGENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGA
                     PWFGAIIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYK
                     VPLYPIIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
BASE COUNT         2293 a         1381 c         1245 g         2710 t
ORIGIN      
        1 gaatgtgcaa cgatttctgg aataacacgc tttacaggta ctgtcactgt tgatgttgtt
       61 ccatcttgat aagtaacagt tacagttact tgtgcgttat ttcctgtacg actcacatta
      121 ctcactgttt tactagtgat ttcatttgca cttgctgttg catagtttct atttggtgca
      181 ttgcttgtaa acgttaatga attaataatt gctgtttgat cagcttgcga tactgtcgca
      241 ttattcgaaa tattggcaat tctcactgga ttagcagccg ttgatgatgt tccaactcga
      301 tatttatcac gtaaaggttt aattgtgaca ttgaatgacg ttgttgcagt attaccgctt
      361 gtatcagttg caaccaaatt aattgtttta tttgtagtag atgtcacatt tggtgctgtt
      421 gctgaaacat gttgatgatt attatcaaca gtgcctgtca attgtgatgt cgttggtact
      481 gttacagatt gcacaccaaa attatcattc gctgtaatcg tattggtaaa tgtttcacca
      541 gagaatactt gatgatttgt gtaattacct actgaaatcg ttggatttac tagatcacta
      601 ggtacaaaat aaatgttaat tgcttcattc gtattgtccg tattttgatt taaatgttca
      661 acatattgtt ttggtccata tggcgataag tataactgcg ctttataaac tgcatcagct
      721 gcattatgcg tagaattatt ttttactaca tgatctattg taaatgcacc cgcaccatta
      781 gctgctgatt cattaacact aacgacagtc gagttactgt ggttcgcacg gacatttgaa
      841 tttgtaacag gttgcccacc aaatccattt gaaggattaa taatatccat tatttgaatg
      901 acttgaccat tcaacgtaaa ttgtcgttga ccatcagatg ttggatttga ttgtgaataa
      961 ccagttgttt tatagtttgg tctaccacca gcttctgctt gtgctttttc attaccagca
     1021 atgttattac gtaatccagg caccgttgtt gtcgctttta acaaagttaa aatatcattc
     1081 gtcgtaattt gcgtacctaa tgttgcatca tagcgataaa ttaatgattt atgatcatca
     1141 gaccatgata aaccaccatc agtcggtcta tttgcaattt ttgcgtttgt ccatgtcact
     1201 gtataatctt gagggaagtt aacccaacgt aagaagttca tattattaag atttgatgtg
     1261 aacgatttcg ttaatcgacc tttatttata ttgacagtaa tggtactcac attagaacca
     1321 ttcggtgtat ttgttactga aatatctcct tctgcaaatg aaatcgcatc tctattggca
     1381 ttgataaatg cttgtttcac ttttgaaatt tcatcagtag ttatatcata tgttgacata
     1441 cgatatacac gttgatcacc ttctggttgt ttcacatcaa tgattttcaa agtgatatag
     1501 aaatcttcag tgtctttatg atacgcattt gtagcaacta tatgatatag ctgtgtgcca
     1561 gcgttcattt gactaacttc ttgcgcagtt ggaatcgttc tatccgatgt aatgtttgta
     1621 aatgaactgc ttggaatcgc ttgtccatta cttaaagtga aatattgttt gatatctcca
     1681 atattaaaag attgcccttt agcaattgta accgttgccg ttttagcagc attagcagtt
     1741 tgatattcag gcttaacaac tggttcgtat gtcatcacgt ttgtcgcatc aagcgtagtt
     1801 gatgacccat cacgatattg caatgtgaca ttaccatgat tatctactgt aactggattt
     1861 gatgaagcta aataacctct tatattttgg tttttattca taaacgcagt agtaatacgt
     1921 tgcttttcat caccagttaa agcagtcgga tttacaacga caactttttc agaatttcct
     1981 aagacaattg gatgcgcatc atcactaatt ttacctacat gcactgtcac atgtttagac
     2041 tgactatcat tcgtcgtatt attgacattg tcggttgctg acactttgaa tgtaatatca
     2101 ctattatagg cctgattcat agagactcta cctgaaatag ctaatgagcc gtttttcttg
     2161 tcggatttag ttaaatttga tgtccaacca cttggcaatg tcgtaatagt tgtacttttt
     2221 aatccagaat gtctatctga aacccccata gtaaagctaa cttcatcgcc acgataatat
     2281 ttcgcattta atccaactgg gttattaatt gtcggtggaa tatcatcagg taaaatagtt
     2341 actgcattac tcatatttga ggcaactaca ttattgtaaa atattaacgc cctaatttga
     2401 ttacctaccg gaggtataaa tactacataa tcttctggtc gtatcgttat cgttgaatga
     2461 accccatcag gtgtacccgt cgcaattttt gtataacctg atggtatttg atctgcctca
     2521 gaaccaccat ctcttgtttg agacgtaggt ataactaaat atactaatgc agatggatcc
     2581 aatggaatat ccgtaacggt tacaggtacc ttttgtaatg ctgtacctct taattcttct
     2641 gctgttgtag taattctagg cgtatgtggc ttaacacgaa tctcgaataa ttgttcttta
     2701 cgtccaaatc gtattgttcc aaagtaatcg cctggtaata aattaccaac cagttcaata
     2761 ttacgttctc tagcattatt agttctattt gttgaattct caatgtgtaa tgtacgtgga
     2821 atacctgtaa tttgccaatc tttatctgta aagttttcat tacttgttaa attaatacgt
     2881 aacggttgta tagatgttac actaccacct aagtttactg aagcattccc ttgattacct
     2941 gcatatacat agaaaaccgg atgtccagct tgttttgaag caggtttatc agcaatattt
     3001 tgaccgtcat catttttaaa gaatttaatg ttatgttgaa tttcaggtaa tactgttaag
     3061 ttaacaggca ctaaagtttc accgttacca aaagcatcag gatagtcgaa accaatcatt
     3121 aatcgtattg ggctgccagt tctagctgcc tgttgcaatt tatcatcagt aaataattga
     3181 cttaattgat attcatttaa tggattgact ccacccggtc taaaagcgtt agtgtttgca
     3241 ctagttggat aaaaacctaa tactttatta cttggtgtct taacataact taatggatca
     3301 tccataaagt catctatatt taggttcgta ctttctaata ccttttttgt ttgtacattt
     3361 ccttgactca cttctactcg tttagttgta cctatacgat aagtacgatt aaccacttgt
     3421 cttaaaccat taagacgttg ttgatagtct tgtgttgatt cgacatataa ctgactatac
     3481 ataaataatt gattgtattc taaagcacgc ccacctgctg catagtacag cttatcaatt
     3541 gttggacctt gcgttttaaa tgaaatcgta taacttgcat cacctttacc aataaatgta
     3601 taaacttttg gtacaccagg tgattctgtc ggcctttcac cattaatata ttcgaatgtt
     3661 tgtctaccaa aagcttttgt agcaggtgtt gctccagctt cactaaaata gtcacttgca
     3721 cgtgctaaat cctttaagct attaatatgg acagttggcc aatcataaat cacctgccca
     3781 ctcctatttc taattttgta gtcccacgaa cgtgagtcgt ttaccccata tggccacatt
     3841 tctggaagtg gtttgttcgc accggctcct gcaccgttac tccattgtac atttgtgccg
     3901 tctgcattca ctgttacaaa ctccgttcga ccaactggag tttgtccggc cggtagtgca
     3961 aaccagtaga tcatattttc atgtggtgca tgacctttgt taaacttaat cacccattga
     4021 taaccgtctc cagtttgctt tgctgtaata agtgcgattt cttttgcgtc tgatgctttt
     4081 aacgtattcc atggcgtagt aggtaaatta tatggtgccc ccttactaga agcattaata
     4141 tatgtaccta caggataccc agaaccatca ttagcatttt caacgtaata tgatgagttt
     4201 gcgttttgat agtctgatac tgatcttggt tctgcagctc ttgattcaat cgaacgcgtt
     4261 tgaattctac ttgagcgtcg acttgtttgt tgacgttgag gcattgtacg aattaaagca
     4321 acagcttgtt ctaattcttt gtatgcaagt tgcaagtctt tattgtttat tggatcatta
     4381 ctacctaata acgtattgat cttgtcaaaa atgcctttta catagttaaa tgtcccagaa
     4441 tctgcatttt cgcggtcaat aaagcgataa tcatggtaat tagcatcaaa aaatgcttgt
     4501 aattcattac gatcgtgttg tacgttacca ctatctgatg aatctgaagc tgtgactaca
     4561 tttgcttcat gactttctac atgtgtcgct ttattgtcgt tttccttttt gttagcagcg
     4621 cgactttcgc tttgttcatg cttacttgcc gctttagccg attgtgttgg cgtagcagct
     4681 gccgtatctt tctttgcttg ctcattttga gatactggtt ggtcatgttc tgttgtagat
     4741 gattgtactg cactagcatc tacatggttt gtttgattat tatttggttg ttcattttca
     4801 ataggcgcag ataatgattg accattttgt gaattttgaa cttctacttc tcgattctca
     4861 gtctgcgtat caccattttg ttgtttctgt tttaatgcac tagctggttg atttgtctca
     4921 gcagcttgtg cttgtgatgt gtttaatcct agaaatacta acgtcgcaat gaccgtcgag
     4981 aatgttccaa cggtatactt acgaatacta aacttttgaa ttttatcacg ataattcaca
     5041 gctattgttc actccttatc ttgttgttat gtctctgttg tgttgtgaat gtatttacat
     5101 ttgatttcat gtgtattgta atgctcattc cattctcatt taaaaattta aaacaatgac
     5161 aattcaatac aatttcgcaa tctatctaaa aagtattgaa atacattaca actatgtgtt
     5221 gaacaattta tttattctcc ataatataat aacacagaat atattggtag gtaaattgaa
     5281 tttgtgacaa tttataataa ttataaataa agtagtgtaa aagggtaata aaaaaccgac
     5341 cttatgtaga atcatttata atgattacac ataagaatcg gtttatattt ttgtttaata
     5401 ttgtagcgac ctttatgatt tcaatttaat tctcaattat cataattgag tgtcgttttg
     5461 tttaggcaca agtaacaaaa tgataacgaa tgataaaatt cccatacctg cgtttaacca
     5521 taatgcaatc attgcaccag tatcaatgtt tgtcatattt gatacgattg cataaactgc
     5581 accactcaat gcgacgccaa atgctcctcc taatgcagaa gccattttat agatacctgc
     5641 agctacgcct actttttcta acggtgcatt tgcaattgct gtatctgttg atggtgtagc
     5701 atatattcct agtcctaaac caaagaataa ataacctata atacaacaaa tgacatataa
     5761 tatttctggc aaaaatgtta atgaaatgag acattctccg acaataagaa ctgctgttcc
     5821 aattagcatt ggtttcttgc atccgaatgt ttgaagtaac ttttcaccaa cacgaatcat
     5881 aattagtacc attactaaat aagtgattga taaacttcct gcttgcaagg aagaatatcc
     5941 taaacctctt tgaacaaatg tgttggctac tattaatgtt cctgcaacac catttaacaa
     6001 aaagtttgaa gctgttgcac ctgtataagc tttattttta aataatttaa aatcgattaa
     6061 aggatttgta gcacgctttt caagaactat aaataaacta aaagatacaa ttgcaattgc
     6121 taatatagtg ataaaaagaa gtgaggtaac acctaattct gatcctttag taattaaaat
     6181 atttaaactg agaagcataa tgactaaaag aaccagacct ttaatgtcaa atttatttag
     6241 agaaatcgat ttagatttag tttcaggtgt gcctttaata agaaacagcg caattaatga
     6301 aattataatt gataagatga aaatccaacg ccaacctaaa agtgttgcaa ctgcacctcc
     6361 aaaaaatgaa caaacaccag agccgcccca tgagccaatt gaccaataac ttaaagcgcg
     6421 ttgtctatct ttcccaatgt aatatgactt aataatagac aaagttgcag gcataataca
     6481 tgctgctgaa agtccttgaa ttaatcttcc tataataagt aataaaggaa tatttgaaat
     6541 aatgattaat aatgaaccca atatatttaa gataatacca atgttcgtga gtttaattct
     6601 gccatattta tcagcaagac cacctgctcc tactacaaac attcctgaaa ataaagcagt
     6661 tatactaaca gcgatattaa ccgttccaat atctgtatta aaactatctt caagtattgg
     6721 tacaacatta accaatgatt gtgcaaatag ccaaaacgtt attacactta aaacaattcc
     6781 tattaacaac ttattattgc cttcaaatgt ctctcttgac ggcttttcca tctctatttg
     6841 cctccctata cttttgaaaa acagacaaat tccataacag aaatttgtct gttcgataga
     6901 ataattgaat ggtttttgat tttaatgtgc aaaactttaa atttattagt agtccattca
     6961 attattctca atgctagtta tcttacctta tgctgctttt tgtttcttct tatagtaata
     7021 aactggtata ccaagtgctg ttattagaat cccaataatt gccaatataa attgtgtaaa
     7081 cagtgtatta attaatacaa atgatcctgc caaaatagca attaaaggta tgattggata
     7141 taacggtact ttatatggtc gttccatatt tggttcacgt tttcttaaaa ttattaccgc
     7201 aacaaatgac atacaataga acaaccaaat aacaaaaatt aacatgtttg taattgtatc
     7261 aaaggcaccc attgacatca tgatgatagc gattataagt tgtataattg cgccaaacca
     7321 tggtgcacca gattttgtta attttgcgaa taagtggcta aatggcaata attttctttc
     7381 agccattgca tatggtacgc gcataccagt catagtatag ccattgatcg taccataaac
     7441 agatattaat ataccgattg tgataatctt accgccattt tctccaaata atatttttga
     7501 tgtatctgat gctgcattta aatttcctgc aagtagttct attggcagtg ttaataagaa
     7561 tgtagcgtta attaataaat acacagccat aatacaaccg ataccaactg aaatcgctaa
     7621 aggtaaatc
//
LOCUS       DAMZPP010000134         7557 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMZPP010000134 DAMZPP010000000
VERSION     DAMZPP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.134"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..178)
                     /locus_tag="POH73_002275"
     CDS             complement(<1..178)
                     /locus_tag="POH73_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6888197.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGAR"
     gene            373..1986
                     /gene="bshC"
                     /locus_tag="POH73_002276"
     CDS             373..1986
                     /gene="bshC"
                     /locus_tag="POH73_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HDI6888198.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQISFEKRMS
                     KENNGREAALANVIREYMNDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQKRTQNQGLKAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFRLNKTDKKYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFIGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASDQFIEKVEGMIEQQRRLYQDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            2130..2561
                     /gene="mraZ"
                     /locus_tag="POH73_002277"
     CDS             2130..2561
                     /gene="mraZ"
                     /locus_tag="POH73_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDI6888199.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            2577..3512
                     /gene="rsmH"
                     /locus_tag="POH73_002278"
     CDS             2577..3512
                     /gene="rsmH"
                     /locus_tag="POH73_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDI6888200.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            3526..3927
                     /gene="ftsL"
                     /locus_tag="POH73_002279"
     CDS             3526..3927
                     /gene="ftsL"
                     /locus_tag="POH73_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDI6888201.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            3908..6142
                     /locus_tag="POH73_002280"
     CDS             3908..6142
                     /locus_tag="POH73_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDI6888202.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVIDMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            6434..7399
                     /locus_tag="POH73_002281"
     CDS             6434..7399
                     /locus_tag="POH73_002281"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDI6888203.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            7401..>7557
                     /gene="murD"
                     /locus_tag="POH73_002282"
     CDS             7401..>7557
                     /gene="murD"
                     /locus_tag="POH73_002282"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDI6888204.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDL"
BASE COUNT         2757 a         1019 c         1482 g         2299 t
ORIGIN      
        1 ttctagctcc taattgatct ccgacaaata tgccataaaa tggtgaatct gaacttgcgt
       61 caatcatttg accgtttaac tcattgacca tgtataagtc tttgttgcca aacgctctaa
      121 agttttcgaa taattcgtca gttttgtaat taatttcaag acgtttgatt tcactcatat
      181 ttatctaccc cttatttgtt tactactatt attatacatt aaaccacttg tgtttttcat
      241 tgatttgaaa atgtaaaaca atgaatatta taaattttcc atgattaaat tgattttcaa
      301 gccgtaaaat tgtcaatcac cggtatagac tttatactat agtttgtaat cgcgtactta
      361 aggagaaaaa acatggactg taaagtagtt agtttaaatg aaaaagatca gtttatacca
      421 aaaataaaga gcagtgatcc tgtaataaca ggattatttc aatatgatgc agctcaacaa
      481 attagttttg aaaaaagaat gtctaaagaa aataatggaa gagaagcggc attagcgaat
      541 gttattcgtg aatatatgaa tgatttaaag ctttctagtg aacaagaatt aaacatacaa
      601 catttagcta atggttcaaa agttgtgatt ggtggacaac aagcagggct tttcggggga
      661 ccattgtata cattccataa aatattttca atcattacct tatctaagga attaacggat
      721 acacataagc aacaagtagt accagttttt tggattgcag gagaagatca tgatttcgat
      781 gaagtgaatc atacatttgt ttataacgaa aatcatgggt cactgcataa ggttaaatat
      841 catacaatgg agatgccaga gacgactgtc tctagatatt atcctgataa ggctgagttg
      901 aaacaaactt taaaaacgat gttcattcat atgaaagaaa ctgttcatac acaaggtcta
      961 cttgagattt gtgacagaat tattgaccaa tatgactcgt ggactgatat gtttaaagca
     1021 ctactgcatg aaacatttaa agcatatggc gttctattta tagatgcgca gtttgagccg
     1081 ttaagaaaaa tggaagcgcc tatgtttaaa aagattttga aaaaacatca gttgcttgat
     1141 gatgctttta gagcaacaca aaaacgtact caaaatcaag gcttgaaggc gatgatacaa
     1201 acagatacaa atgttcattt attcttacat gatgaaaata tgcgtcaatt agtttcgtat
     1261 gatggtaagc attttagatt aaataaaaca gataagaaat atataaagga agaaattatt
     1321 aatattgcgg aaaatcaacc tgaattattt tctaataatg tagtgacaag accattaatg
     1381 gaagaatggc tatttaacac ggtggcattt attggagggc cgagtgaaat taagtactgg
     1441 gctgaactaa aagatgtatt tgaactattt gatgttgaaa tgcctatcgt gatgccaagg
     1501 cttagaatta cttatttaaa tgaccgtatt gaaaaattac tttcgaaata caatattcca
     1561 ttagaaaaag tgttagtcga tggtgttgaa ggagaaagaa gtaagtttat tagagaacaa
     1621 gcatcagatc aatttattga aaaggtagaa ggtatgatag aacaacagcg tcgtctatac
     1681 caagacttat tagatgaagt ggcggggaat caaaataata ttaaccttgt gaataaaaat
     1741 aatgaaattc atatacaaca gtatgattat ttgttaaaac gttatctttt aaacattgaa
     1801 agagaaaacg acatcagtat gaagcaattt agagaaattc aagaaacact ccatccaatg
     1861 ggaggattac aagaaagaat atggaatcca cttcaaattt tgaatgattt tgggacagat
     1921 gtgttcaagc cctccaccta tccaccactt tcttacactt ttgatcatat tattataaaa
     1981 ccttaatata ccaagggttt agcccgattt atcataatga taaatcgggc atttttttgt
     2041 tttttaaaat aaatttcaca aattttgtat aaatagtggt ggatagtggg gagatgtggt
     2101 aaattatata taaggtgagg tgataaaaaa tgttcatggg agaatacgat catcaattag
     2161 atacaaaagg acgtatgatt ataccgtcca agtttcgtta tgacttaaat gagcgtttta
     2221 ttatcacaag aggccttgat aaatgtttat tcggttacac tctagacgaa tggcaacaga
     2281 ttgaagagaa aatgaaaacc ttacctatga caaaaaaaga cgcacgtaag tttatgcgta
     2341 tgttcttctc tggtgctgtt gaagtagaac ttgataagca agggcgtatt aacatccctc
     2401 aaaacttgag gaaatacgct aatttaacta aagaatgtac agtaatcggt gtttcaaatc
     2461 gtattgagat ttgggataga gaaacttgga atgatttcta tgaagaatct gaagaaagtt
     2521 tcgaagatat tgctgaagat ttaatagatt ttgattttta aaatggagga attgaagtgt
     2581 ttcatcatat cagcgttatg ttaaacgaaa ccattgatta tttaaatgtt aaagaaaatg
     2641 gtgtgtacat tgactgtacg ctaggtggag cgggacatgc cctttattta ctaaatcaat
     2701 taaatgacga cggaagatta atagcaatcg atcaagacca aactgcaatt gataatgcta
     2761 aagaggtatt aaaggatcat ttgcataagg tgacttttgt tcatagcaac ttccgtgaat
     2821 taactcaaat attaaaagac ttaaacattg aaaaagtaga tggaatctat tacgacttgg
     2881 gtgtttcaag cccacaactc gacattccag aacgaggatt cagttatcac catgacgcaa
     2941 cattagacat gcgtatggac caaacacaag aactaacagc atatgaaatt gttaacaatt
     3001 ggtcatatga agcgttagtg aagatttttt atcgctatgg cgaggagaaa ttttcaaaac
     3061 agatagctcg aagaatcgaa gcacatcgcg aacaacaacc aataacaaca acattagaat
     3121 tagttgacat tataaaagaa ggtattcctg caaaagcaag aagaaaaggc ggacatcctg
     3181 caaaacgagt atttcaagca ctacgaattg cagtaaacga tgaattgtca gcttttgaag
     3241 attcaataga acaagcgatt gaattagtga aagtagacgg caggatttcg gtaatcactt
     3301 tccattcttt agaagatcgt ttatgtaaac aggtgttcca agaatatgaa aaaggtccag
     3361 aggtaccaag aggattacca gttataccag aagcatatac acctaagtta aagcgtgtta
     3421 atcgtaaacc gattaccgct acagaagaag atttagatga caataacaga gcacgaagcg
     3481 cgaaattacg tgtagctgaa atacttaaat aaggagcaat taataatggc tgtagaaaaa
     3541 gtgtaccaac catatgacga acaagtttat aatagtatac cgaagcaaca accacaaact
     3601 aagcccgaaa agaagactgt ttcgagaaaa gtggttgtac aattaactaa atttgaaaaa
     3661 gttttataca taactttgat tactgtaatt gctatgttaa gtatttatat gctatcttta
     3721 aaaatggatg cgtatgatac gcgaggaaag attgcagatt tagattataa aatagataaa
     3781 caatcaagtg aaaacagtgc tttacaatct gaaatcaaaa agaattcttc ttatgaacgc
     3841 atatacgaaa aggctaagaa acaggggatg agccttgaga acgataatgt aaaggtagtg
     3901 cgtagtaatg gcgaagcaaa aaattaaaat taaaaaaaat aaaatagggg cagtcctact
     3961 tgttggttta ttcggactgc tcttttttat attggtttta agaatttcat atatcatgat
     4021 tactggacat tctaatggtc aagatttagt catgaaggca aatgagaagt atttagttaa
     4081 gaatgcacaa caaccagaac gaggaaagat atatgatcgt aatggtaaag tgctagcaga
     4141 agatgtagaa agatataaac ttgttgcagt aatagataaa aaggcgagtg ccaattctaa
     4201 aaaacctagg catgtagttg ataaaaaaga gactgcaaag aaattatcta cagtcattga
     4261 tatgaagcca gaggaaattg aaaagagact tagtcaaaag aaagccttcc aaattgaatt
     4321 tggacgcaaa ggaacaaatt taacgtatca ggacaaattg aaaatagaga aaatgaattt
     4381 gcctggtatt tctttattgc ctgaaacaga acgattttat ccaaatggca attttgcatc
     4441 acacttaatt ggtagagctc agaaaaatcc ggatactggt gaacttaaag gtgcgcttgg
     4501 agttgaaaag atttttgata gttatttaag tggatctaaa ggatctttga gatatattca
     4561 tgatatttgg ggatatattg caccaaatac taaaaaagag aagcagccta aacgtggtga
     4621 tgatgtccat ttaacaatcg attcaaatat tcaagtattt gttgaagagg ctttagatgg
     4681 catggttgaa agataccagc cgaaagattt atttgcggtt gtcatggatg ccaaaactgg
     4741 agaaatttta gcatacagtc agcgaccaac atttaatcct gaaactggta aagattttgg
     4801 taaaaagtgg gcaaatgatc tatatcaaaa cacatatgag cctggatcaa catttaaatc
     4861 atatgggtta gcagctgcta ttcaagaagg tgcttttgat cctgataaga aatataaatc
     4921 tggacataga gatattatgg gttcacgtat ttcagactgg aatagagtcg gttggggtga
     4981 aatcccaatg tcactcggat ttacttattc atctaataca ttgatgatgc atttacaaga
     5041 tttagttggt gcagacaaaa tgaaatcttg gtatgaacga tttggatttg gaaaatcaac
     5101 taaaggtatg tttgatggag aagcacctgg tcaaattgga tggagtaatg agttacaaca
     5161 aaaaacgtca tcatttggtc aatcgacaac tgtaacacct gttcaaatgt tacaggcgca
     5221 atcagcgttc tttaatgatg gtaatatgtt aaaaccatgg tttgtgaata gcgttgaaaa
     5281 tcctgttagt aaaagacaat tttataaagg tcaaaaacaa atcgcaggca aaccaataac
     5341 aaaagatact gctgaaaaag ttgaaaagca attggattta gttgtgaata gtaagaagag
     5401 tcacgctgca aactatcgta ttgatggtta tgaggtcgaa ggtaagactg gtacagcaca
     5461 agtcgctgca cctaatggtg gtggatacgt taaaggtcca aacccatatt tcgtaagttt
     5521 tatgggtgac gcaccgaaga aaaatcctaa agttattgta tacgctggta tgagcttggc
     5581 acaaaaaaat gaccaagaag cttatgaatt aggtgttagt aaagcgttta aaccaataat
     5641 ggaaaatact ttgaaatatt taaatgtagg taaatcaaaa gatgacacat ctaatgcaga
     5701 gtatagtaaa gtgccagatg ttgaaggtca agacaaacaa aaagctattg ataatgtgag
     5761 tgcaaaatca ttagaaccag ttactatcgg ttctggcaca caaataaaag cgcaatctat
     5821 aaaagcaggg aataaagtct tacctcatag taaagtactg ttattaacag atggagactt
     5881 aactatgcct gacatgtcag gatggacgaa agaagatgtc attgcttttg aaaacctaac
     5941 aaatattaaa gtgaatttaa aaggtagcgg ttttgtgtcc caccaatcaa ttagtaaggg
     6001 acaaaaactt actgaaaaag ataaaataga cgtagaattt tcatcagaga atgtagacag
     6061 caattcgacg aataattctg attcaaattc agatgataag aagaaatctg acagtaaaac
     6121 tgacaaagat aagtcggact aacaagaatg gctacattaa ataagggtgt attgtcacta
     6181 tttgttttta ctaaagtaat gctgtgatag aaaacaacat cttcatttaa tctttataaa
     6241 taagtgttga tagagaattt taaggtagac gaggtcagat taacttgttt gttataggca
     6301 aactttttat actggcctct tatgccgtaa aaaagaaaaa ttataaattt tgattacaac
     6361 tttgtttatt taattgaaaa attgataata tattagtggt taaaaaagtc tggattaaga
     6421 agaggagata gttatgattt ttgtatatgc gttattagcg ctagtgatta catttgtttt
     6481 ggtacctgtt ttaataccta cattaaaaag gatgaaattt ggtcaaagta ttcgagaaga
     6541 aggcccacaa agccatatga agaagactgg tacaccaacg atgggtggac taacatttct
     6601 attaagtatt gtgataacgt ctttggtggc tattatattt gtagatcaag ctaatccaat
     6661 catactgtta ttatttgtga cgattggttt tgggttaatt ggttttatag atgattatat
     6721 tattgttgtt aaaaagaata accaaggttt aacaagtaaa cagaagtttt tggcgcaaat
     6781 tggtattgcg attattttct ttgttttaag taatgtattt catttggtga atttttctac
     6841 gagcatacat attccattta cgaatgtagc aatcccacta tcatttgcat atgttatttt
     6901 cattgttttt tggcaagtag gtttttctaa tgcggtaaat ttaacagatg gtttagatgg
     6961 attagcaact ggactgtcaa ttatcggatt tacaatgtat gccatcatga gctttgtgtt
     7021 aggagaaacg gcgattggta ttttctgtat cattatgttg tttgcacttt taggattttt
     7081 accatataac attaaccctg ctaaagtgtt tatgggagat acaggtagct tagctttagg
     7141 tggtatattt gctacgattt caatcatgct taatcaggaa ttatcattaa tttttatagg
     7201 tttagtattc gtaattgaaa cattatctgt tatgttacaa gtcgctagct ttaaattgac
     7261 tggaaagcgt atatttaaaa tgagtccgat tcatcatcat tttgaattga taggttggag
     7321 tgaatggaaa gtagttacag tattttgggc tgttggtctg atttcaggtt taatcggttt
     7381 atggattgga gtgcattaag atgcttaatt acacagggtt agaaaataaa aatgtattag
     7441 tcgtcggttt ggcaaaaagt ggttatgaag cagctaaatt attaagtaaa ttaggtgcga
     7501 atgtgactgt caatgatggg aaagacttat cacaagatgc tcatgcaaaa gatttag
//
LOCUS       DAMZPP010000135         7541 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMZPP010000135 DAMZPP010000000
VERSION     DAMZPP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.135"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..1105
                     /locus_tag="POH73_002283"
     CDS             <1..1105
                     /locus_tag="POH73_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDI6888205.1"
                     /translation="QNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISGKLPKN
                     YKDELPRFEFGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDATDYSSE
                     TPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIMGLNAT
                     FIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALESESTPT
                     SLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVEAAKDL
                     EKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVGTAGKV
                     IAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            1383..1625
                     /locus_tag="POH73_002284"
     CDS             1383..1625
                     /locus_tag="POH73_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDI6888206.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            1612..1710
                     /locus_tag="POH73_002285"
     CDS             1612..1710
                     /locus_tag="POH73_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888207.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKDKYVY"
     gene            1804..2271
                     /locus_tag="POH73_002286"
     CDS             1804..2271
                     /locus_tag="POH73_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HDI6888208.1"
                     /translation="MLYLIFSIIVALFMGTLVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLVIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRVAMLYKYKKLKKTLIN"
     gene            2395..3516
                     /locus_tag="POH73_002287"
     CDS             2395..3516
                     /locus_tag="POH73_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDI6888209.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIMISGNHDGKERLNYGASWFENNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEGAINILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFSYNEENNAIEISE
                     KDDMSIIEMFYNHITDKELSDIQSNKIKNILENELRKED"
     gene            3520..6549
                     /locus_tag="POH73_002288"
     CDS             3520..6549
                     /locus_tag="POH73_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDI6888210.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNNRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKENAQIENRYQQIDLLWQEIESFDDD
                     KIKGLLEVATQQIDKVIENIPLLQARSKEILAFVNESKETAIKDYEIIEKKTLENNIL
                     KDNINQLNKNKIDFVQLKEQQPEIEEIEAKLKLLQDITNLLNYIENREKIETKIANSK
                     KDISETNNKILNLECDKRNIDKEKRMLEENGDLIESKISFIDKTRVLFNDINKYQQSY
                     LNIERLRTEGEQLADELNNLIEGLEKVEDSIGNNESDYEKIIELNNAITNINNEINVI
                     KENEKAKAELDKLLGSKQELENQINEETSTLKNLEIKLDRYDKSKLDLNDKESFISEI
                     KSAVKIGDQCPICGNEIQDLGHHIDFDSIAKRQNEIKEIEANIHTMKSNIAVHNSEIK
                     FVNEKISNINIKTQSDFSLEVLNKRLLENENALNNQRELNKFIEQMKEEKDNLTLQIH
                     NKQLRLNKNESELKLCRNLITEFETLSKYNNITNFEVDYKKYVQDVNQHQEHSNQIED
                     KLIQLSQRKLIEQNNLNHYEKQLETYNNDLELNEQSIEMEMSRLNLTDNNDINEIIAW
                     RGEQEELEQKRDTYKKRYHEFEMEIARLESLTKDKELLDSDKLKDEYELKKGKMNTLI
                     DEYSAVHYQCQNNINKTQSIVSHINYLNQELKDQQEIFQLAEIVSGKNNKNLTLENFV
                     LIYYLDQIIAQANLRLATMSDNRYQLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLS
                     GGETFQSSLALALGLSEIVQQQSGGISLESIFIDEGFGTLDQETLETALDTLLNLKST
                     GRMVGIISHVSELKNRIPLVLEVKSDQYQSSTRFKRN"
     gene            complement(6620..6982)
                     /gene="mscL"
                     /locus_tag="POH73_002289"
     CDS             complement(6620..6982)
                     /gene="mscL"
                     /locus_tag="POH73_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDI6888211.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
     gene            7186..>7541
                     /locus_tag="POH73_002290"
     CDS             7186..>7541
                     /locus_tag="POH73_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDI6888212.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMA"
BASE COUNT         2902 a          979 c         1324 g         2336 t
ORIGIN      
        1 ccaaaatact atgttaaaac gtgctaatga agatgaatct caatggaatt cattattaga
       61 aaaatatgca gaaacatatc ctgaattagc agaagaattt aaattagcga ttagtggtaa
      121 attgcctaaa aattataagg atgaattacc acgttttgaa tttggtcata atggtgcatc
      181 tcgtgctgat tctggtactg ttattcaagc aatcagtaaa actgtccctt cattctttgg
      241 tggatcagca gaccttgctg gttcaaacaa atccaatgta aatgatgcaa cagattatag
      301 ttctgaaaca cctgaaggta aaaatgtgtg gtttggtgta cgtgaatttg ctatgggtgc
      361 tgctgtaaat ggtatggctg cacatggagg tttacatcca tatggtgcaa cattcttcgt
      421 atttagtgat tatttaaaac cagcgttacg tttatcatca attatgggat taaatgcaac
      481 gttcatcttc acgcatgatt caattgcagt aggtgaagat ggtcctactc atgaaccaat
      541 tgaacaatta gctggattaa gagccattcc aaatatgaat gttatccgtc ctgctgatgg
      601 taatgaaaca agagtagcat gggaagttgc cttagaatct gaatctacac ctacttcatt
      661 agtattgaca cgtcaaaact taccggtttt agatgtacca gaagatgtag ttgaagaagg
      721 cgttcgaaaa ggtgcctata cagtttatgg ctctgaagag acaccagaat ttctattatt
      781 agcttcaggt tcagaagtta gtcttgcagt tgaagctgct aaagatcttg aaaaacaagg
      841 taaatcagtg cgtgttgttt caatgcctaa ctggaatgca tttgaacaac aatctgaaga
      901 atataaagaa tcagttattc catcaagcgt aacaaaacgt gttgcgattg aaatggcttc
      961 accgcttgga tggcataaat atgtaggtac tgcaggtaaa gttattgcta ttgacggctt
     1021 tggcgcaagt gcacctggcg atttagtagt tgaaaaatat ggctttacaa aagaaaatat
     1081 cttaaaccaa gttatgagct tataagaata atttataaag cgagtatgtt tagaagtcta
     1141 ggatgcataa tcttaggctt cttttaaagt gttgaaattt agagtatcgc actcaaacta
     1201 catcataagt gataagtttt gaaaatatac tatttcagat taatctttaa aacctctgtt
     1261 ataacagcat gatttttgat attattttta gtatcgatat taaaatactt gaataaacta
     1321 gttcttgaaa taatgtgatg aatttagtaa aattcagtag gataaagaaa gcgggtgaaa
     1381 caatggcaac ttggttagca attattttta tagtagctgc attaatttta ggtttaattg
     1441 gaggtttcct tttagctaga aaatatatga tggactactt gaagaaaaac ccaccaatca
     1501 acgaagaaat gcttcgtatg atgatgatgc aaatgggtca aaaaccttct cagaagaaaa
     1561 ttaatcaaat gatgacgatg atgaataaaa atatggatca aaatatgaag agtgcgaaaa
     1621 agtaaattcg caattgatag aggctatttt ccagatatgg aaatggcctc tttttataat
     1681 caaattaata aggataaata tgtttattaa aattaaagtt aacaaaatga cgaatagact
     1741 gagaaatgct ataattcatt ttgtatgatt tacagagagt ttatttaacg agaaggtgtc
     1801 tgcgtgctct atttaatatt ttcaatcatt gtagctttat ttatgggaac tctagttata
     1861 gttattcgta tgaaagctca aaattatccg gtaaatgaga aaaaaatagt tttgccaccg
     1921 ttttttatgg cgactggtgc attgatgtac gtcgttccat attttaggct aacaggatcg
     1981 gaaatgctag aagcctttat aattggtttg cttttttcta cagttctaat ttggacttct
     2041 cgatttgaag tcaaaggtac agaaatttat atgaaacgat ctaaagcatt tccagttatt
     2101 ttgatttcat tacttgtcat tcgtactgtg atgaaaatat tcattagtaa tgaaatagat
     2161 cctggagaat taggcggcat gttcttttta ttagcattct gtatgattgt tccttggaga
     2221 gtagcaatgc tatataaata caaaaaacta aagaaaacat taatcaatta attacttcta
     2281 aaaccacttg tgatcgactt ctaaatcagt caatgagtgg ttttaatttt acttgaaaag
     2341 gtgaaatgat tatattaaaa taaaagcgaa caaatgttct atatggaggc agtaatgaaa
     2401 attatacata cagcagactg gcacttaggg aaaatattaa atggcaaaca gcttttagaa
     2461 gatcaagcgt atattttaga tatgttcgta gaaaaaatga aagaagaaga acctgatatc
     2521 attgtgatag ctggagattt atatgacaca acatatccaa gtaaagatgc aatcatgtta
     2581 ttagaacaag cgattggaaa gctgaattta gaactgcgta taccaataat tatgattagc
     2641 ggaaatcacg atggtaaaga gagattaaac tatggggcga gttggtttga aaataatcag
     2701 ttatttataa gaacagattt tacatcgatt aattcaccaa tagagataaa tggggttaat
     2761 ttttatacac tcccttatgc tactgtgagc gaaatgaaac actattttga agatgacacc
     2821 attgaaacgc atcaacaagg aattacgcgc tgtattgaaa caatagcacc ggaaattgat
     2881 gaaggtgcca tcaatatttt aattagtcat ctgactgttc aaggtggaaa gacatctgat
     2941 tctgaaagac cattaactat tggaacggtt gaatcagttc agaaaggtgt ttttgatata
     3001 tttgattatg tcatgctagg tcacttgcat catccattta gtatagaaga cgacaaaatt
     3061 aaatatagtg gctccttatt gcagtattca ttttcggaag cgggtcaagc aaaagggtat
     3121 agacgtgtaa caattaatga tggcattatt aacgatgtat ttattcctct taagccgctt
     3181 agacaattgg aaattatctc gggcgaatat aatgatgtta ttaatgaaaa agttcatgtg
     3241 aaaaataaag ataattattt acattttaaa cttaaaaata tgtctcatat tactgatcca
     3301 atgatgagtt taaaacaaat ttatcctaat actttagcgc tgacgaatga aacttttagt
     3361 tacaatgaag aaaataatgc tatagaaata agtgaaaaag atgacatgtc aatcatcgaa
     3421 atgttttata accatataac tgataaagaa ttatcggata tccaatctaa taagataaaa
     3481 aatattttag aaaacgaatt gagaaaggag gattaatgaa tgaaaccatt acatttaaag
     3541 ttgaataatt tcggcccctt tttaaaagaa gaaattgatt tttctaaaat tgataataat
     3601 gaattgtttt taataagtgg taagactgga tcgggtaaaa caatgatttt tgatgcaatg
     3661 acttatgcct tgtttggtaa agcatcaact gaacaaagag aagaaaatga tttgagaagt
     3721 catttcgctg atggtaaaca gccgatgtca gtaacgtttg aatttcaatt aaataatcga
     3781 atttataaag tgcatagaca aggcccttat atcaaagaag gtaatacaac aaaaacgaac
     3841 gctaaatttg atgtatttga gatggtggat ggcaagtatg aaattagaga aagtaaagta
     3901 atttcaggta cccaattcat tattgaatta ttaggagtaa atgcagatca attccgacaa
     3961 ttgtttattt tgcctcaagg tgaattcaaa cgctttttaa tatcaaacag tcgtgaaaag
     4021 caagggatat taagaacgct gtttgacagt gaaaaatttg aagctatacg agaaatatta
     4081 aaagaagaag taaaaaaaga aaatgctcaa atcgagaata gatatcaaca aattgacctt
     4141 ttatggcaag aaattgaatc atttgatgat gacaaaataa aaggcttatt agaggttgcc
     4201 actcaacaga tagacaaagt gattgaaaat ataccacttt tacaagctag gtcgaaagaa
     4261 atactagcat ttgtaaatga aagtaaagaa actgctatta aagactatga aataatagaa
     4321 aagaaaacac tagaaaataa tatattaaaa gataatatta atcaactcaa caaaaataaa
     4381 attgatttcg ttcaattgaa agagcaacaa cctgaaatag aggaaattga agctaagtta
     4441 aagttgttac aagatattac aaacctattg aattatattg aaaatagaga aaaaattgaa
     4501 actaaaatcg ctaatagcaa aaaagatatt tctgaaacca ataataaaat attgaatctt
     4561 gagtgtgata agcgaaacat agacaaagag aaaagaatgt tagaagaaaa cggagattta
     4621 attgaaagta aaatctcttt tattgataaa actagagtat tatttaacga tattaataag
     4681 tatcaacaaa gttatctcaa tattgaacgc ttgagaactg agggtgaaca attagctgat
     4741 gaattaaata atctaattga aggtttagaa aaggtcgaag attcaatagg taataacgaa
     4801 agtgattacg agaaaattat cgaactaaat aatgcgataa ctaacataaa taatgaaatt
     4861 aatgtaatta aagaaaatga aaaagctaaa gctgaattag ataaactatt aggtagtaag
     4921 caagagttag agaatcaaat taatgaagaa acatctacat tgaagaatct cgaaataaaa
     4981 ttagatcgct acgataaatc aaaattggac ttaaatgata aagaaagttt tataagtgaa
     5041 attaaatctg ctgtaaagat tggagatcaa tgtccgatat gtggtaatga aattcaggat
     5101 ttagggcatc atattgattt tgacagtatc gctaaacgtc aaaatgaaat taaagaaatt
     5161 gaagcaaata tccacactat gaaatcgaat attgctgtac ataattctga aattaaattt
     5221 gttaatgaaa aaatatcgaa tattaatatt aaaacgcaaa gtgatttctc actcgaagta
     5281 ttgaataagc gtctgctaga aaatgaaaat gcattgaata atcaaagaga gcttaataaa
     5341 tttatagaac aaatgaaaga agaaaaagat aatttaacgt tgcaaattca taataaacaa
     5401 ttgcgtctaa ataaaaatga atctgagttg aaattatgtc gaaatctcat cactgaattt
     5461 gaaacactct cgaaatataa taatatcact aattttgagg tggattataa gaagtatgta
     5521 caagatgtga atcaacatca agaacactca aaccagattg aagataagtt aatacaatta
     5581 tctcaaagaa agttaattga gcaaaataat ctaaatcact atgaaaaaca actagaaact
     5641 tacaataatg acttagaatt gaatgaacaa tctattgaaa tggaaatgtc gaggctgaat
     5701 ttaactgata acaatgatat aaatgaaata atagcctgga ggggcgagca agaggaatta
     5761 gagcagaaaa gggatactta taaaaaacgt tatcatgaat ttgaaatgga aatagctagg
     5821 ttagaatcat taaccaaaga taaagagtta ttggactctg ataaattaaa agatgaatat
     5881 gaactaaaaa aaggaaagat gaatacactg atagatgaat actctgctgt tcattatcaa
     5941 tgtcaaaata atattaataa aacacaatct atagtttcgc atattaatta cttaaatcaa
     6001 gaattaaagg atcaacaaga aatatttcaa ttggctgaga ttgtcagtgg taagaataac
     6061 aaaaatctta cattggaaaa cttcgtctta atttactatt tagatcaaat tattgctcaa
     6121 gcaaatctga gattagcgac aatgtcagat aatcgatacc aactaattag gcgagaagcg
     6181 gtttctcatg gtcttagtgg cctagaaatt gatgtatttg atttgcattc aaataagtct
     6241 agacatatta gctcgttatc aggtggagaa actttccaat cgtcgcttgc attagcttta
     6301 gggttaagtg aaattgtaca gcagcaatca ggaggtattt cactagaatc aatttttatt
     6361 gatgaaggat tcggtacatt agatcaagaa acgcttgaaa cagcgttaga cactttgtta
     6421 aatcttaaat caactggtag aatggttggg attatttcac atgtgagcga attgaaaaat
     6481 agaatacctt tagttttaga agtgaaatca gatcaatatc agagttcaac aagattcaaa
     6541 agaaattaaa acataaaaaa tagagatggt actttggaat gtatagaaca accttagccc
     6601 atctcttttt atttaaaaat tattttttct cacgtaataa atctctgatt tcagttaata
     6661 acacaacatt ttcttccaca actgcttctt cttcggcttc ttctttcttc attaaggtat
     6721 ttgcaatctt aacgaagatg aataaagcaa acgcgatgat aataaagtcg ataacagatt
     6781 ggataaataa accatattta ataccccaga atgaccattc tttagcaaaa tcaactgatc
     6841 cgaaaatttt accaattaat ggcataatga tattttctac taatgaagat ataatcttgt
     6901 tgaaagctgc acccatcaca acagcaattg ctaaatctaa gacgttacct tttaaggcga
     6961 actctttgaa ttcttttaac attacactca acctctcttg tttaaatgta acctcaatta
     7021 tacaacaaat aaaaaaatta gaaaatagat tatttaagaa aatttgatta tcgcaaagac
     7081 ctttgttatc ctataaaggt gcgtgagtta ttgaataaaa tgtttagata atatttttat
     7141 tctactacat aaaattaaat atagaattga agggagtaat taattatgaa ttcttcttca
     7201 ccagagaatc caaatggaaa gaagtattca ccagtcttca tctatagtgc aattgttgtt
     7261 gctatagtcg tattacttgg tgcattttta cctgaacaat tcaactatgt taccaataat
     7321 attaaaatgt ggattacaga aaagttaggt tggtattatc ttattcttac tacgattatc
     7381 gtgttcttct gtatattcct tatttttagt cctattggaa aacttaaact aggtaaacca
     7441 aatgacaaac ctgagtttaa tacaatttca tggtttgcta tgttgtttag tgctggtatg
     7501 gggataggtt tggtgtttta tggtgcagct gaaccgatgg c
//
LOCUS       DAMZPP010000136         7485 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMZPP010000136 DAMZPP010000000
VERSION     DAMZPP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.136"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..420
                     /locus_tag="POH73_002291"
     CDS             <1..420
                     /locus_tag="POH73_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6888213.1"
                     /translation="SASDSDSDSDSDSDSESDSDSDSDSDSDSDSASDSDSDSDSASD
                     SDSDSTSDTGSDNDSDSESDSNSDSDSGSNNNVVPPNSPKSGTNASNKNEAKESKEPL
                     PDTGSEDEANTSLIWGLLASLGSLLLFRRKKENKDKK"
     gene            641..2148
                     /gene="vwb"
                     /locus_tag="POH73_002292"
                     /pseudo
     CDS             641..2148
                     /gene="vwb"
                     /locus_tag="POH73_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor binding protein Vwb"
     gene            2499..3524
                     /gene="emp"
                     /locus_tag="POH73_002293"
     CDS             2499..3524
                     /gene="emp"
                     /locus_tag="POH73_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HDI6888214.1"
                     /translation="MKKKLLVLTMSTLFATQFMNSNHANASTESVDKNFVVPESGINK
                     IIPTYDEFKKAPKVNVSNLADNKNFVASEDKLNKIADPSAASKIVDKNFAVPESKLGI
                     IVPEYKEINNRVNVTTNNPASKQVDKQIVAKDPEVNRFITQNKVNHRFITTQTHYKKV
                     ITSYKSTHVHKHVNHATSSIHHHFTIKPSEAPRYTHPSQSQSLIINHHFAVPGYHGHK
                     VVTPGQASIRIHHFCAVPQINSFKVIPSYGHNSHRMHVPSFQNNTTATHQNAKVNKTY
                     NYKYFYTYKVVKGVKKHFSFSKSHGCKIVKPALNIKNVNYQYAVPSNSPTHVVPEFQG
                     ILPAPRV"
     gene            3855..4412
                     /locus_tag="POH73_002294"
     CDS             3855..4412
                     /locus_tag="POH73_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888215.1"
                     /translation="MKRKVLVLTMGVLCATQLWQTNNAKALVTESGVNDTKQFTEVTS
                     EEKVIKDAISKVNESFIYYPQNDLKGLGGEHNDYEKITYSTSSNNVLELSMSSKYVGG
                     KSGAMVGYSEIYSSHFTDRDKRAIRRDHVKEAQNLINDYKYTQIYEDFAKATAKVSTL
                     SQSHQNYLNKQIDKVNNKIEKTEKR"
     gene            4753..5439
                     /locus_tag="POH73_002295"
     CDS             4753..5439
                     /locus_tag="POH73_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDI6888216.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKEQYAKRFFFFATSCL
                     VLTLVVASSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
     gene            5796..5996
                     /locus_tag="POH73_002296"
     CDS             5796..5996
                     /locus_tag="POH73_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDI6888217.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
     gene            complement(6493..6711)
                     /locus_tag="POH73_002297"
     CDS             complement(6493..6711)
                     /locus_tag="POH73_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888218.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEIDLDN
                     GNVSNIDHSLLVSVDPKALEISIFDAVN"
     gene            complement(6773..7057)
                     /locus_tag="POH73_002298"
     CDS             complement(6773..7057)
                     /locus_tag="POH73_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888219.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNNGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            complement(7145..>7485)
                     /locus_tag="POH73_002299"
     CDS             complement(7145..>7485)
                     /locus_tag="POH73_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888220.1"
                     /translation="DKPTTQSFESFKYNYASGKNDWMKVYRCSYPGAGKKAADWVKKN
                     YENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSVKSYGLHIISPYAIKDNFID
                     PYKLRLVKAY"
BASE COUNT         2946 a         1047 c         1188 g         2304 t
ORIGIN      
        1 agtgcctcag attcagatag cgactcagac tcagacagcg attcagaatc agatagtgac
       61 tcagattcag atagcgattc cgactcagac agtgcctcag attcagatag tgactcggat
      121 tcagcgagtg attccgattc agattcaacg agtgacacag gatcagataa cgactcagac
      181 tcagaaagtg attcgaatag cgattccgat tcaggttcta acaataatgt agtgccgcct
      241 aattcaccta aaagtggtac taatgcttca aataaaaatg aggctaaaga aagtaaagaa
      301 ccattaccag atacaggttc tgaagatgaa gctaatacgt cactaatttg gggattatta
      361 gcatcattag gttcattact acttttcaga agaaaaaaag aaaataaaga taagaaataa
      421 gtaataatga tattaaatta atcgtatgat tcatgaagaa gtcacctaaa aaggtggctt
      481 ttttacttgg attttccaaa tatattgttt gaatataatt aataattaat tcatcaacag
      541 ttaattattt taaaaaggta gatgttatat aatttggctt ggcgaaaaaa tagggtgtaa
      601 ggtaggttgt taattaggga aaattaagga gaaaatacag ttgaaaaata aattgctagt
      661 tttatcattg ggagcattat gtgtatcaca aatttgggaa agcaatcgtg cgagtgcagt
      721 ggtttctggg gaggagaatc catataaatc tgagtcattg aaattaaatg ggaaaagaag
      781 tactacaata actagtgata aatatgaaga aaatttagat atgttaatat cgtcattatc
      841 atttgcagat tatgaaaaat atgaggaacc agaatacaaa gaagcagtta aaaagtatca
      901 acaaaaattt atggctgaag atgatgcatt aaaaaatttt ttagtgaaga gaaaaaaata
      961 aaaaatagaa atactaatac atcaaattat ctgggattaa cacacgaaag atatgagtca
     1021 atttataatt cattaaaaaa tcatcgtgaa gaattttcaa aagaaatcga agaaattaat
     1081 aataaaaatc cagtgttaaa agaatataac aatgaggaac aaactaaagc tgatacggaa
     1141 ttaaacactc ttgaaaatca agtactaatg ataggttata cattttatca ctcgaataaa
     1201 aatgaagtag aagatttata taacaaatta gatatgattc ttggttataa agatgaagag
     1261 agaaaaaaga agagggctac caatcaaaga atgttcaata ataaaaaaga ggatttagaa
     1321 actattattg atgaattctt tggagaaatt ggacaacaaa ggccaacatc tataccaaca
     1381 ttagcgccta aagaagaaaa agaaacaaat ataaaaaatg caaataaatt aaaatctgac
     1441 actgaagcag caaaaaatga tgaagcaaaa agaagtttaa atacccacaa tcacaaatct
     1501 gtatctcaag aagtctctga acaacaaaaa gctgactacg aaagaaaagc tgaagaaaga
     1561 aaagcgagat ttttagataa gcaaaaaaat aagaaaactc ctgtagtttc attagaatat
     1621 gattttgaac ataaacaacg tgttgacaac gaaaacgaca agcaacttgt ggtttctgag
     1681 ccatcaaaga aaccaacaac accgcctaca tacactgaaa caaccacaca gctaccaatg
     1741 cctacagttg agcgtcaaac acagcaacaa atcgtttaca aagcaccaaa accattagct
     1801 ggattaaatg gtgaaagtca tgatttcaca acaacgcatc aatcaccaac tacttcaaat
     1861 cacacgcata atcatcttat tgaaattgaa gaaacatctg ctttacctgg tagaaagaca
     1921 ggttcattgg ttggtttgag tcaaattgat tcttcgcatt taactgaacg tgagaagcgc
     1981 gtgattaaac gtgaacacgt gagagaagct caaaagttag ttgataatta taaagataca
     2041 catagttata aaggccgatt aaatgcccaa caaaaagtaa atactttaag tgcaggtcat
     2101 caaaaacgtt ttaataaaca aattaataaa gtatataatg gcaaataatt aatgcatggc
     2161 tgcaaaggaa ataatgagtt tgccgtaaaa ataacaacat tttaaactag caataaataa
     2221 tatcaaagtc atcatttcaa tgatgcaatc tagtatagtc cacattctaa acaggtgtgg
     2281 actattactt ttttcacttt atattacgaa aaaattatta tgcttaacta tcaatatcaa
     2341 taattaattt taagctgaaa aacaataaaa atgttaagac aacgtttact tcaagttaat
     2401 tattatactg aaaattctgg tatataatgc tgttagtgaa tataacagga aaattaaatt
     2461 ggttatgata ttgagtctat ataaaggaga aataacagat gaaaaagaaa ttattagttt
     2521 taactatgag cacgctattt gctacacaat ttatgaattc aaatcacgct aatgcatcaa
     2581 cagaaagtgt tgataaaaac tttgtagttc cagaatcggg tattaataaa attattccaa
     2641 cttacgatga atttaaaaaa gcaccaaaag taaatgttag taatttagct gacaacaaaa
     2701 actttgtagc ttctgaagat aaattgaata agattgcaga tccatcggca gctagtaaaa
     2761 ttgtagataa aaactttgcc gtaccagaat caaaattagg aatcattgta ccagagtata
     2821 aagaaatcaa taatcgagtg aatgtaacaa caaacaatcc agcttcaaaa caagttgaca
     2881 agcaaattgt tgctaaagac ccagaggtga atagatttat tacgcaaaat aaagtaaacc
     2941 atcgtttcat tactacgcaa acccactata agaaagttat tacttcatac aaatcaacac
     3001 atgtacataa acatgtaaac catgcaacat cttctatcca tcatcacttt actattaaac
     3061 catcagaagc acctagatat acacacccat ctcaatctca atcgttaatt ataaatcatc
     3121 attttgcagt tcctggatac catggtcata aagttgtaac accaggacaa gctagtatta
     3181 gaattcatca cttttgtgct gtacctcaaa taaatagttt taaggtcatt ccatcatatg
     3241 gtcacaattc acatcgtatg catgtaccaa gtttccaaaa taacacaaca gcaacacatc
     3301 aaaatgcaaa agtaaataaa acttataact ataaatattt ttatacttat aaagtagtca
     3361 aaggtgtaaa aaaacatttc tcattttcaa aatcacatgg ttgtaaaatt gttaaaccag
     3421 cattaaacat caaaaatgta aattatcaat atgctgttcc aagtaatagc cctacacacg
     3481 ttgttcctga gtttcagggt atcttaccag caccacgagt ataaaaattg acattaagtt
     3541 tacgagatat gataaatacc tattatttta aacatagtct gcaatctatg aggttgtagg
     3601 ctatgttttt tgcagtttat caataaacac ccatcaacaa attataccgt ttttctactt
     3661 taaaagttgg aagtaacata atcttaaata aatatattat taattaagat aaatataaga
     3721 ctcgagatta ttgttaatag tttgttcatc gcaagttaat tattgtttct aaaatattgg
     3781 tatataattt tcaatggcga agaaaacagg gtaaaaaagt cggtttttaa atcaaagcaa
     3841 ataaggagta aaaaatgaaa aggaaagtac tagtattaac aatgggcgta ctttgtgcga
     3901 cacaattatg gcaaacgaat aatgcaaaag ctttagtgac agagagtggc gttaatgata
     3961 ctaagcaatt tactgaagta acatcggaag aaaaagttat aaaagatgct atttcgaaag
     4021 tcaatgaaag ctttatttac tatccccaaa atgatttgaa gggattaggt ggagaacaca
     4081 acgattacga aaaaattaca tatagcactt cttctaataa tgttttagaa ttatcaatga
     4141 gttcaaaata cgtaggcggt aaatcaggag ctatggttgg ttatagtgaa atttactcat
     4201 cacatttcac agaccgcgac aaacgtgcta tcagacgtga tcatgttaaa gaagcacaaa
     4261 acttgattaa tgattataaa tatacgcaaa tatatgaaga ctttgctaaa gctactgcaa
     4321 aggtaagtac acttagtcag tctcaccaaa attatttaaa taaacaaatt gataaagtga
     4381 ataataagat agagaaaact gaaaaacgct aatacaaagt aatttataag ttatacattt
     4441 cgtttttaaa tgacaattta tccccgtaaa tataataaat aatcttttca aattccgcat
     4501 agatatagag acgctaataa acctctttgt ctcgatatga tagtctgcaa cgattcatgt
     4561 tgtaggcttt ttaattttac aaatacgact aaatatataa gttctgacac ctaaaatata
     4621 gaaaatacat aaaagtaagt atagttattt tattataatt attaaatttt tattaattaa
     4681 ttgtaaaaat gtagaattat aattaattaa cgtttaatat taaaattaac taaaaagaaa
     4741 gaggtgttag ttatgacaga atacttatta agtgctggca tatgtatggc aattgtttca
     4801 atattactta tagggatggc tatcagtaat gtttcgaaag aacaatacgc aaagaggttt
     4861 ttctttttcg ctactagttg cttagtgtta actttagttg tagcttcaag tctaagtagc
     4921 tcagcaaatg catcacaaac agataatggc gtaaatagaa gtggttctga agatccaaca
     4981 gtatatagtg caacttcaac taaaaaatta cataaagaac ctgcgacatt aattaaagcg
     5041 attgatggtg atactgttaa attaatgtac aaaggtcaac caatgacatt cagactatta
     5101 ttggttgata cacctgaaac aaagcatcct aaaaaaggtg tagagaaata tggtcctgaa
     5161 gcaagtgcat ttacgaaaaa gatggtagaa aatgcaaaga aaattgaagt cgagtttgac
     5221 aaaggtcaaa gaactgataa atatggacgt ggcttagcgt atatttatgc tgatggaaaa
     5281 atggtaaacg aagctttagt tcgtcaaggc ttggctaaag ttgcttatgt ttataaacct
     5341 aacaatacac atgaacaact tttaagaaaa agtgaagcac aagcgaaaaa agagaaatta
     5401 aatatttgga gcgaagacaa cgctgattca ggtcaataat gctcattgta aaagtgtcac
     5461 tgctgctagt ggcactttta taatttttag atcacgttat gatttattat caaatcagaa
     5521 ttaaaaaagt aaatcgtatc aaaatcaagt gtatttaata ttagaaaata aaaattttaa
     5581 atttagtatt aaaatggaat attactatat atttcaatgt gtattatcac agaaaataaa
     5641 ataatgcttt acttctatat ttaaaagtgt ataatgaagg ttaagtaata aagagcgtga
     5701 agaaaaatgt gagttattta tatagaatat tctccttttc atttatgaat ttgttacaaa
     5761 atatttagtg caaaagcacg acggaggtat tcaatatgaa taacggtaca gttaaatggt
     5821 ttaatgcaga aaaaggtttt ggtttcatcg aaagagaaga tggtagcgac gtattcgtac
     5881 acttctcagc aatcgctgaa gatggataca aatcattaga agaaggccaa aaagttgaat
     5941 tcgacatcgt tgaaggcgac cgtggcgagc aagctgcaaa cgtagttaaa atgtaatttt
     6001 aacttattca aacagtcctt attatagggc tgttttttta tgctttaaat cgatagcagt
     6061 tggtgtggta aaagcactag ctgttatttt tttgtccaat aaatttaggt ggagatctaa
     6121 caatatataa tggttctaaa ataaatcgaa ctgatggaaa agttttttac ttttcatctg
     6181 tccgactttt gattttgaat ataaaaaaag cgccaataca gaactttaat aatgacgaga
     6241 attaaagtct gtatatggcg ataacaagaa gtaatgttaa acactcaaaa tgtttaacaa
     6301 taataggata ccacatcgca taatatctta ctacttaatt aataatttaa ctaatcaact
     6361 ttttgttaat tttttattaa gactgattaa ttattgagaa tatttattgt ttttaaaatc
     6421 tcataataat tcagtaatct tgttttcatt taaaaggcga aacattaaaa taattaaata
     6481 aaaatatcgt gtttaattta cagcgtcaaa tatacttatt tctaatgctt tggggtctac
     6541 tgaaacaagt agagaatgat cgatgttact aacattgcca ttatccaaat ctatttctgt
     6601 gagtatttgg aagctaccat taggcaacgg tttaacaata gacaattgct tttccgcttg
     6661 ttgtattaaa aaaggttttg tagattgatt attaatatgc cattcactca tgtatgtttt
     6721 tcactcctgc tttaaattag ggttagaaag tttatagttg agacattcat gttcaaccaa
     6781 aattttgttc aaattcaata aatgtcttgt ttaaaataga aatattgtaa atgttatcgt
     6841 ccaaaacttc accagttaag tatttgtttt gaattaaaat ttggcagtta gttaagaagt
     6901 cttgataatc acgatcgcaa aaatagtttt cacgtgcatc tttagcatcg ccaaaaaagt
     6961 tagcgactgt ttctgtttct ccattattcg aacgttcaat atataatttg taaaatttag
     7021 ctattgtata cttttgttct ttagttagtt cattcaaaat attgggcctc ctgaaatatc
     7081 atttgtaatc tatacccaat ttattgcaaa acacaaacta atttaactat ttgatgaaac
     7141 tgtgttaata agctttaaca agtcttagtt tatatggatc tataaaatta tctttaattg
     7201 catagggtga aataatatgt agtccataac ttttaactga tttttcactt acaccaaatt
     7261 tataagcttg atagataatt ttagtacaat acgtaaattt tttgctgttc aaatttaatg
     7321 taactagata acgatgattt gtattttcat agtttttctt aacccagtca gccgcttttt
     7381 tacctgcacc aggatagctg caacgataaa ctttcatcca atcatttttg ccacttgcat
     7441 aattatattt aaaagattcg aaggattgtg tagttggttt gtcgc
//
LOCUS       DAMZPP010000137         7383 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMZPP010000137 DAMZPP010000000
VERSION     DAMZPP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.137"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..792
                     /locus_tag="POH73_002300"
     CDS             <1..792
                     /locus_tag="POH73_002300"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDI6888221.1"
                     /translation="LKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAGINIEAM
                     MIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFNEWWKQL
                     FGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDITIEKDSD
                     DLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVVYFFELA
                     CAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            1118..1693
                     /locus_tag="POH73_002301"
     CDS             1118..1693
                     /locus_tag="POH73_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDI6888222.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVDTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLIVFWIVGKKLEQHFMGSKKE"
     gene            1698..2222
                     /gene="lepB"
                     /locus_tag="POH73_002302"
     CDS             1698..2222
                     /gene="lepB"
                     /locus_tag="POH73_002302"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDI6888223.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKKLDGDIIPPNNFVVLNDHDNNQHDSRQFGLIDKKDIIGNISL
                     RYYPFSKWTIQFKS"
     gene            2238..2813
                     /gene="lepB"
                     /locus_tag="POH73_002303"
     CDS             2238..2813
                     /gene="lepB"
                     /locus_tag="POH73_002303"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDI6888224.1"
                     /translation="MKKELLEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIIGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKYNFNPENTKN"
     gene            2973..6449
                     /gene="addB"
                     /locus_tag="POH73_002304"
     CDS             2973..6449
                     /gene="addB"
                     /locus_tag="POH73_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDI6888225.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLESFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDALQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEISNELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQINPMLR
                     LLKTDVLTTSYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAATVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGNVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLDDEIVADSWLDAYQVIRDSDHLSQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQAMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWADIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            6450..>7383
                     /locus_tag="POH73_002305"
     CDS             6450..>7383
                     /locus_tag="POH73_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDI6888226.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYETGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDGVDKHLAVIEDERRLMGRVLEGGFIDISYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYK"
BASE COUNT         2666 a         1032 c         1346 g         2339 t
ORIGIN      
        1 ttaaaacagt tggctacaaa cgaaggttat gaaacgttta tcgtacctga tgatgtaggt
       61 ggaagatatt ctgttttaac agcagtagga ttattaccaa ttgcaacagc tggaattaac
      121 atcgaagcta tgatgattgg tgctgcaaaa gcacgtgaag aattatcttc agataaatta
      181 gaagaaaaca ttgcatacca atatgcgaca attcgaaaca ttttatatgc aaaaggttat
      241 acaacagaaa tgttgattaa ctatgaacca tctatgcaat actttaatga atggtggaaa
      301 caattatttg gtgaatcaga aggtaaagac ttcaaaggta tttatccttc aagtgccaac
      361 tacacaactg atttacattc tttaggtcaa tatgtacaag aaggccgtcg tttcttattc
      421 gaaacagttg taaaagtaaa tcatcctaaa tatgatatta ctattgaaaa agatagtgat
      481 gatctagacg gattaaatta tttagctggt aaaacaatcg atgaagttaa cacaaaagca
      541 ttcgaaggta cattattagc gcatactgat ggtggtgttc ctaacatggt agtgaacatt
      601 ccacaattag atgaagaaac tttcggttat gtcgtatact tcttcgaact tgcttgtgca
      661 atgagtggat accaattagg cgtaaatcca tttaaccaac ctggtgtaga agcatataaa
      721 caaaacatgt tcgcattatt aggtaaacct ggttttgaag acttgaaaaa agaattagaa
      781 gaacgtttat aaaatacatt acttcaatga ttagtgaagt ttgaaaagat agaactagac
      841 gttaactatt taaagcatat ttccgaggtt gtcattacaa atgtaaaaat gtaatgacaa
      901 cctcgttttt attttatatg caagatatag gttactagct aatgtgacaa gatgttaaga
      961 gaaaattaaa gagaaaataa tatctgccat acaataaaat tgttatacta ctagagactg
     1021 atttattagc atgattacat gttaatgttt ctttacttag taattaactt tataatgtaa
     1081 gaataattat cttcagccaa agaaagggat tgatgatttg tcgtttcatc aagtagaaga
     1141 atggtttgag atatttcgac agtttggtta tttacctgga ttcatattgt tatatataag
     1201 agcgattatt ccagtatttc cattagcact ctatatttta attaatattc aagcctatgg
     1261 acctatttta ggtatattga ttagttggct tggattaatt tctggaacat ttacagtcta
     1321 tttgatctgt aaacgattgg tggatactga aaggatgcag cgaattaaac aacgtactgc
     1381 tgttcaacgc ttgattagtt ttattgatcg ccaaggatta atcccattat ttattttact
     1441 ttgttttcct tttacgccaa atacattaat aaattttgta gcgagtctat ctcatattag
     1501 acctaaatat tatttcattg ttttggcatc atcaaagtta gtttcaacaa ttattttagg
     1561 ttatttagga aaggaaatta ctacaatttt aacgcatcct ttaagaggga tattaatgtt
     1621 agttgtgttg attgtatttt ggattgttgg aaaaaagtta gaacagcatt ttatggggtc
     1681 gaaaaaggag tgacatcgtg aaaaaagttg taaaatattt gatttcattg atacttgcta
     1741 ttatcattgt actgttcgta caaacttttg taatagttgg tcatgtcatt ccgaataatg
     1801 atatgtcacc aacccttaac aaaggggatc gtgttattgt aaataaaatt aaagttacat
     1861 ttaatcaatt gaataatggt gatatcatta catataggcg tggtaacgag atatatacta
     1921 gtcgaattat tgccaagcct ggtcaatcaa tggcgtttcg tcagggacaa ttataccgtg
     1981 atgaccgacc ggttgacgca tcttatgcca agaacagaaa aattaaagat tttagtttgc
     2041 gcaattttaa aaaattagat ggagatatta taccgcctaa caattttgtt gtgctaaatg
     2101 atcatgataa caatcagcat gattctagac aatttggttt aattgataaa aaggatatta
     2161 ttggtaatat aagtttgaga tattatcctt tttcaaaatg gacgattcag ttcaaatctt
     2221 aaaaagaggt gtcaaaattg aaaaaagaat tattggaatg gattatttca attgcagtcg
     2281 cttttgtcat tttatttata gtaggtaaat ttattgttac accatataca attaaaggtg
     2341 aatcaatgga tccaactttg aaagatggcg agcgagtagc tgtaaacatt attggatata
     2401 aaacaggtgg tttggaaaaa ggtaatgtag ttgtcttcca tgcaaacaaa aatgatgact
     2461 atgttaaacg tgtcatcggt gttcctggtg ataaagtaga atataaaaat gatacattat
     2521 atgtcaatgg caaaaaacaa gatgaaccat atttaaacta caatttaaaa cataaacaag
     2581 gtgattacat tactgggact ttccaagtta aagatttacc gaatgcgaac cctaaatcaa
     2641 atgtaattcc aaaaggtaaa tatttagtgc ttggggataa tcgtgaagta agtaaagata
     2701 gccgtgcgtt tggcctcatt gatgaagacc aaattgttgg taaagtttcg tttagattct
     2761 ggccattcag cgaatttaaa tataatttca atcctgaaaa tactaaaaat taatatgaaa
     2821 caaatacaac atcgtttgtc ggttttaata ctgataaacg atgttttatt ttgtaatagg
     2881 tatgataaaa gctaagttcg aaatgaactt ataataaatc aatcacaatc actttgtgtt
     2941 aaaatatgtg tcaaaggaag tgagggtttg tcatgacatt acatgcttat ttaggtagag
     3001 cgggaacagg taagtctacg aaaatgttga ccgaaataaa acaaaaaatg aaagcagatc
     3061 cacttggaga tccaatcatt ttaattgcgc caactcaaag tacatttcaa ttagaacaag
     3121 cctttgtcaa tgatccggaa ttaaatggta gtttaagaac agaagtgttg cattttgaac
     3181 gattaagtca tcgtattttc caagaagttg gtagttatag cgaacaaaag ttatctaaag
     3241 ctgcaacgga aatgatgatt tataacattg ttcaagaaca acaaaagtat ttaaaacttt
     3301 atcaatcaca agcaaaatat tatggattta gtgaaaaatt aacagaacaa attcaagatt
     3361 ttaaaaaata tgcagtaacg cctgaacatt tagaaagctt tattgctgat aaaaatatgc
     3421 aaactcgaac taaaaataag ttagaggata ttgctttaat ataccgagag ttcgaacaac
     3481 gcattcaaaa cgagtttatt acgggtgagg atgcattaca atattttatt gattgtatgc
     3541 cgaaatcaga gtggctaaaa cgtgctgata tatatattga tggttttcac aacttttcaa
     3601 cgattgagta tttaataatc aaaggattaa ttaaatatgc gaagagtgtc acaattatat
     3661 tgacgacaga tggtaaccac gatcaattca gtttatttag aaaaccatcg gaagtgttac
     3721 gacatattga agaaatatca aatgaactca atatttctat tgaacgtcaa tatttcaacc
     3781 aattatatcg ctttaataat caagatttaa agcatcttga acaagaattt gatgcacttc
     3841 aaatcaatcg agtggcatgt caaggtcata tcaatatttt agaatctgcg actatgagag
     3901 aggaaataaa tgaaattgca cgacgtatca tcgttgatat tcgtgataaa caattacgat
     3961 atcaagatat tgctatttta tatcgtgacg agtcttatgc ttatttattt gattccatat
     4021 taccgcttta taatattcct tataacattg atacaaagcg ttcgatgaca catcatccgg
     4081 tcatggaaat gattcgttca ttgattgaag ttattcaatc aaattggcaa attaatccaa
     4141 tgctacgctt attgaagact gatgtgttaa cgacatcata tctaaaaagt gcatacttag
     4201 ttgatttact tgaaaatttt gtacttgaac gtggtatata cggtaaacgt tggttagatg
     4261 atgagctatt taatgtcgaa cattttagca aaatggggcg taaagcgcat aaacttaccg
     4321 aagatgaacg taacacattt gaacaagtcg ttaagttaaa gaaagatgtc attgataaaa
     4381 ttttacattt tgaaaagcaa atgtcacaag cagcaactgt aaaagacttt gcaactgctt
     4441 tttatgaaag tatggaatat tttgaactgc caaatcaatt gatgacagag cgagatgaac
     4501 ttgatttaaa tggtaatcat gaaaaggcgg aagaaattga tcaaatatgg aatggcttaa
     4561 ttcaaatcct cgatgactta gttctagtat ttggagatga accaatgtcg atggaacgtt
     4621 tcttagaagt atttgatatt ggtttagaac aattagaatt tgttatgatt ccgcaaacat
     4681 tggaccaagt tagtattggt acgatggatt tggctaaagt cgacaataag caacatgttt
     4741 acttagttgg aatgaatgat ggtacgatgc cacaaccagt aactgcgtca agcttgatta
     4801 cagatgaaga aaagaaatac tttgaacagc aggctaatgt cgagttaagt ccaacatcag
     4861 atattttaca gatggatgaa gcatttgttt gttatgttgc tatgactaga gctaagggaa
     4921 atgttacatt ttcttatagt ctaatgggat caagtggtga tgataaggaa atcagcccat
     4981 ttttaaatca aattcaatca ttgttcaacc aattggaaat tactaacatt cctcaatacc
     5041 atgaagttaa cccattgtca ctaatgcaac atgctaagca aaccaaaatt acattatttg
     5101 aagcattgcg tgcttggtta gatgatgaaa ttgtggctga tagttggtta gatgcttatc
     5161 aagtaattag agatagcgat catttaagtc aaggtttaga ttatttaatg tcagcattaa
     5221 cgtttgacaa tgaaactgta aaattaggtg aaacgttgtc taaagattta tatggtaagg
     5281 aaatcaatgc cagtgtatct cgatttgaag gttatcaaca atgcccattt aaacactatg
     5341 cctcacatgg tctgaaacta aatgagcgaa cgaagtatga acttcaaaac tttgatttag
     5401 gcgatatttt ccattccgtt ttaaaatata tatctgaacg tattaatggt gattttaaac
     5461 aattagacct gaaaaaaata agacaattaa caaatgaagc attggaagaa attttaccta
     5521 aagttcaatt taatttatta aattcttcag cttactatcg ttatttatca agacgcattg
     5581 gcgctattgt agaaacaaca ctaagcgcat taaaatatca aggcacgtat tcaaagttta
     5641 tgccaaaaca ttttgagaca agttttagaa ggaaaccaag aacaaatgac gaattaattg
     5701 cacaaacatt aacgacaact caaggtattc caattaatat tagagggcaa attgaccgta
     5761 tcgatacgta tacaaagaat gatacaagtt ttgttaatat cattgactat aaatcctctg
     5821 aaggtagtgc gacacttgat ttaacgaaag tatattatgg tatgcaaatg caggcgatga
     5881 catacatgga tatcgtttta caaaataaac aacgccttgg attaacagat attgtgaaac
     5941 caggtggttt attatacttc catgtacatg aacctagaat taaatttaaa tcttgggcag
     6001 atattgatga agataaatta gaacaagatt taattaaaaa gtttaagctg agtggtttag
     6061 tgaatgcaga ccaaactgtt attgatgcat tggatattcg tttagaacct aaattcactt
     6121 cagatattgt accagttggt ttgaataaag atggttcttt gagtaaacga ggcagccaag
     6181 ttgcagatga agcaacgatt tataaattca tccaacataa caaagaaaat tttatagaaa
     6241 ccgcttcaaa tattatggat ggacatactg aagttgcacc attaaagtac aaacaaaaat
     6301 tgccatgtgc tttttgtagt tatcaatcgg tatgtcatgt agatggcatg attgatagta
     6361 agcgatatcg aactgtagat gaaacaataa atccaattga agcaattcaa aatattaaca
     6421 ttaatgatga atttgggggt gagcaataga tgacaattcc agagaaacca caaggcgtga
     6481 tttggactga cgcgcaatgg caaagtattt acgaaactgg acaagatgta cttgttgcag
     6541 ccgcggcagg ttcaggtaaa acagctgtac tagttgagcg tattatccaa aagattttac
     6601 gtgatggcat tgatgtcgat cgacttttag tcgtaacgtt cacaaactta agtgcacgtg
     6661 aaatgaagca tcgtgtagac caacgtattc aagaggcatc gattgctgat cctgcaaatg
     6721 cacacttgaa aaaccaacgc atcaaaattc atcaagcaca aatatctaca cttcatagtt
     6781 tttgcttgaa attaattcaa cagcattatg atgtattaaa tattgacccg aactttagaa
     6841 caagcagtga agctgaaaat attttattat tagaacaaac gatagatgag gtcatagaac
     6901 aacattacga tatccttgat cctgctttta ttgaattaac agagcaattg tcttcagata
     6961 gaagtgatga tcagtttcga atgattatta aacaattgta tttctttagc gttgcaaatc
     7021 caaatcctac aaattggttg gatcaattgg tgacaccata cgaagaagaa gcacaacaag
     7081 cgcaacttat tcaactacta acagacttat ctaaagtatt tatcacagct gcctatgatg
     7141 ctttaaataa ggcgtatgat ttgtttagta tgatggatgg cgtcgataaa catttagctg
     7201 ttatagaaga tgaacgacgt ttaatggggc gtgttttaga aggtggcttt attgatatat
     7261 cttatttaac tggtcacgaa tttggtgcgc gtttgcctaa tgtaacagcg aaaattaaag
     7321 aagcaaatga aatgatggtc gatgccttag aagatgctaa acttcagtat aaaaaatata
     7381 aat
//
LOCUS       DAMZPP010000138         7344 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMZPP010000138 DAMZPP010000000
VERSION     DAMZPP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.138"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            443..853
                     /locus_tag="POH73_002306"
     CDS             443..853
                     /locus_tag="POH73_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDI6888227.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            895..1251
                     /locus_tag="POH73_002307"
     CDS             895..1251
                     /locus_tag="POH73_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI6888228.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEHEYRK"
     gene            1372..2340
                     /locus_tag="POH73_002308"
     CDS             1372..2340
                     /locus_tag="POH73_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6888229.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTHYQFKMPVKVDKHYGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVNGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(2421..3356)
                     /locus_tag="POH73_002309"
     CDS             complement(2421..3356)
                     /locus_tag="POH73_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDI6888230.1"
                     /translation="MRYLVNNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(3540..3743)
                     /locus_tag="POH73_002310"
     CDS             complement(3540..3743)
                     /locus_tag="POH73_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888231.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            4293..5336
                     /locus_tag="POH73_002311"
     CDS             4293..5336
                     /locus_tag="POH73_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDI6888232.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            5339..6019
                     /gene="sdaAB"
                     /locus_tag="POH73_002312"
     CDS             5339..6019
                     /gene="sdaAB"
                     /locus_tag="POH73_002312"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDI6888233.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSFRNDGRYIEIIGISIGGGTIKIKGIYINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIMIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            6033..6932
                     /gene="sdaAA"
                     /locus_tag="POH73_002313"
     CDS             6033..6932
                     /gene="sdaAA"
                     /locus_tag="POH73_002313"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDI6888234.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            6951..7049
                     /locus_tag="POH73_002314"
     CDS             6951..7049
                     /locus_tag="POH73_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888235.1"
                     /translation="MIKKKHLSGSLVPDRCFLIIFHKVEKFMGNAC"
     gene            7111..7281
                     /locus_tag="POH73_002315"
     CDS             7111..7281
                     /locus_tag="POH73_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888236.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
BASE COUNT         2599 a         1109 c         1425 g         2211 t
ORIGIN      
        1 gaacttgtca ttgaagatgt ccaagatgct gatccaggta agctaattat gacagtcaaa
       61 taatgattga aaatgaaata gcatatcaaa tgaatgaact tttagtcatt catagtgcgt
      121 atgcgaagta gcgagttgaa agagaatacg ttacaaaagg cagtagctta aaatgaagct
      181 actgtctttt tagtgcgcaa tgatgtatag caggtgtgtt gatgagaata agttaaatat
      241 tagtgttagg tatagaaaac attgcttatg tttttgtcac attttagaaa aatgcatctt
      301 cgcgactagc caaattaata gcgtcattga aaagtttgat tttccgtaat aaagttcaat
      361 tgcttgtatt tttatcatga aagtaataaa aataaattaa catgatttta aatctatttg
      421 taagataagg agatttgtca ttatgacaac agaaggtcta ttagttgcag agaaagaaat
      481 cgaagtgaat ggttacgaca ttgatgcgat gggtgtcgtt agtaatatcg tttatattag
      541 atggttcgaa gatttgagaa cagcgtttat taatcagcac atgaattact caacaatgat
      601 caatcaaggc atttcaccta tacttatgaa aacggaagca gaatataaag tacctgtcac
      661 aatacatgac aaaccagtag gccgtattta cttagttaaa gcaagcaaga tgaaatgggt
      721 gtttcagttt gaaattgtgt ccgcacatgg cgtgcattgt attggtacac agacaggcgg
      781 tttttacaga ttgagcgata agaagattac ctctgtgcca caagtatttc aagacatttt
      841 agcaacaaaa taatgacttc attttaaaat ataaaaagta agaaggtgtt cgaaatggtt
      901 aagcaattaa atagtgtcga agcattccgt gaatttattc atcaatatcc gttagcagtt
      961 gtacatgtca tgcgcgatca gtgtagcgtg tgtcatgccg ttttaccaca aattgaagac
     1021 ttgatgcaat cataccccaa tgtgccatta gctgtgatta atcaaagtca ggtggaagct
     1081 attgctggag aattaaatat tttcactgta cctgtggatt taatttttat gaatggaaaa
     1141 gaaatgcatc gtcaagggcg ttttatcgat atgcaacgtt ttgaacatca tcttaagcaa
     1201 atgaatgata gtgtgaataa cgatgtcgat gagcatgaat atcgcaaatg attagtattg
     1261 ctaagattat gtagacatta taacttattt cccagtaaat attggtagta ataagaatca
     1321 gcatggtaca ttaatactat agtagaaatc atcaaagagg agtgacgaca aatgcgtaaa
     1381 aaatggtcta cacttgcgtt tggattttta gtagcggcat acgcacatat tagaattaaa
     1441 gaaaaacgca gtgtgaaaag ttatatgtta gaacaaggta tacgattatc tagagctaag
     1501 cgtcgtttta tgtataaaga agaagcgatg aaagcattag aaaaaatggc gccacagaca
     1561 gcaggtgaat atgagggcac ccattatcag tttaagatgc cagtaaaagt ggataagcac
     1621 tacggttcaa ccgtttatac cgttaacgat aaacaagata agcatcaacg cgttgtatta
     1681 tatgcacatg gaggcgcatg gttccaagac ccactcaaaa ttcatttcga atttattgat
     1741 gaacttgcag aaacactcaa tgctaaagtc atcatgccag tatatccgaa gattccgcat
     1801 caagattatc aagcgacgta tgtgcttttt gaaaagttgt accatgattt attgaatcaa
     1861 gtagcagatt ctaaacaaat cgttgtaatg ggtgactctg cgggcggtca aattgctcta
     1921 tcatttgctc aattgttaaa agaaaaacat attgtgcaac caggacatat tgtattaatt
     1981 tcaccagttt tagatgcaac gatgcagcat cctgaaattc ctgactactt aaagaaagac
     2041 ccaatggtag gtgtgaatgg cagtgtgttc ttagctgaac aatgggcagg ggacacacct
     2101 ttagataact acaaagtatc gccaattaat ggtgacttag atggtttagg tcgtattaca
     2161 ttaactgttg gcacaaaaga agtactgtac ccagatgctt tgaacttatc gcaattgttg
     2221 agtgcaaaag gtatcgaaca tgactttata cctggatatt accaattcca tatttatcca
     2281 gtatttccga taccagagcg acgacgcttt ttataccaag tgaaaaatat tattaactaa
     2341 aataaaaaac tctgatatca cctataaggt aactacgttg ttaaaaatag ttacttgcta
     2401 aagtgattat cagagttttc ttattcatct aatgtttcag gttgtctttt aagggatagt
     2461 aataaaatta aaatcataat aagaatgatg cctacgattt gaaatggttt gaatgccaca
     2521 tgtaaccata gactgcttgc gataacggct acgacaggtt caacagttcc gaataatgtt
     2581 gtttctttcg ctgatatgta ttgtaaactg tcgataaaga aataaaatgc gagtgcggta
     2641 ccaccgataa taccaaagat taaaaataat ataattgata tgtcccattt agtgatatca
     2701 atgtgccaaa ttggatggcg taaattcatc gcaacaccag aaataagcat tgcccacccg
     2761 acaattagaa tcgaaccgaa gcggttaagt aggtctgaag gataaattgt gtaaaaagcg
     2821 agtgctacac cagctaaaat accccagaat aaacttgcag gattgacgac taaattagaa
     2881 aatgaaccat tcgttaataa taaaaatgtt cctaatagtg tcatgataat agcaagcaca
     2941 tcaaataatg ttagttttgc aacgcctctt atgacaaacc aaataataat atagactggc
     3001 gcaatgtatt gtagtaatgt tgcaatcgca gcattacctg tattaataga tgccatataa
     3061 gcatattgta caactaacat gcccagtata ctgaagataa gtaatttgcc taacatacgt
     3121 cgatcttgga atacgctaaa tatagaacgt ttgggttgca tcattttgta cataataagt
     3181 aagaaaacac cactgactac aagtcgagca gtgacgtacc aatcgacatt aatattctta
     3241 tatttgaaca agaaatcaga aacagtacca cctaatcccc ataaacaagc accgacaatg
     3301 gcaagtataa ttccttttaa acgcaaagct gaatgattat tcaccaagta tctcacccct
     3361 ttaaaactta acaccaacaa cataaaatat gtcagtgtat gataatgatt taattcatta
     3421 tatcgacttt taagaaagag tgaaatgaga aaataaaaaa aatcatctaa gatgtagtgg
     3481 tctggatgat agataaatga atcgatatgg gaaatgaatg ttgaaacaca gaggataaac
     3541 tatttaaatg agatttcagg aagactcaaa tgtttttgcg taagagatcc atgatgagaa
     3601 tgctgactat ctaaaacgaa atgtgataca tcatgtccag tcatactcgt atataaaata
     3661 atgactaaat aagaagtgaa cgttataaag ataagtgcta aatagataga aattgcaact
     3721 ttaacggaat gactcaattg cacctttatc gcctccacta agttatacta aatattttca
     3781 tcttcagtat aaccaaccat tgttaagttg atgtaaattt agctttaaaa aaagtaaaga
     3841 ttcaacctat agactatcat cattcctatc agaaaactaa aaataatatc ctactttaat
     3901 tatcttattg caaatatatg tgtaataagt tatttttgaa tatagtgtgc ggttttataa
     3961 acacagcaca aaaataagaa ataaatagct cgaaagataa aatgtataaa tattaaaagt
     4021 ttttagaaat attacttact ttcagatgta aaaaagttat caaaaaactg tcacacaact
     4081 gtttgaaata tagagtaaaa agttaattag taaatattaa tgacaacata agcagttttt
     4141 gaaacaatcc attttaaaaa tctttcacaa atatgaaata tttcacttaa atatttgatt
     4201 tgataacaga taaaagttga aaacgttttc ttaagggtgt acgatgttta tggtctgaaa
     4261 ataaaataaa catagagaga ggtttttggg aaatggatat tctgttaggt gtaggcacat
     4321 tagtcttggt tttagtgatc atgacgctat ttttaaagta tgcaccgtac ggtaaacaag
     4381 gactacaagc attatccggt gcagcttgtg cgacatttct accacaagca ttcttaagtt
     4441 acgctattgg tggcgtattt gatattaaat ttctacaaga tattggggat ttagcaggaa
     4501 gtttaagcgg tatcgcagta ggtattctaa cgtgcttaaa cttaggcgtt gcaccagtat
     4561 ttgcggtcat tgttggttta gtattacatg actttaaatt attaccagca ttcatcgcag
     4621 cttatggcgt agctttttta attaaatgga ttgaaaagaa agttccagaa ggcttggact
     4681 taattgtggt gatcttattt gcaccagcaa ttgctttcgg acttgcatca atcattacac
     4741 ctggtgtgct agcaacatta aaacaaatag gtagtgctgt cactgcggta ggtgataaca
     4801 atccatatgc attagcggtc attttaggac tagtgattcc tgttacaggt atgacaccgc
     4861 taagttcaat ggtattaaca agtttattag gactgacagg tgttcctatg gctattggag
     4921 cactaacatg tacaggtagc tcatttgtta accttatttt attccgaaaa ttaaacattg
     4981 gtggaccgtc aaaagcattt gcagtttgta ttgagccatt gacgcaaatt gatttaatag
     5041 cgcaatatcc agtacaacta tatggtacaa atgcactgat tggggtcgtc aacgcatgta
     5101 tcgtgacatt tagcggacta gtaataggtg tcaaaggtat ggcaacacca attgcaggtg
     5161 ccatcgtatt atttggcttt aataatgcag tgacatcaat tgtaacaatc gcaacagtga
     5221 ttattgtcag cattgtctta gcctatatta ttggaacact aattaataag tttaatttaa
     5281 tgaatatcaa ctttaaaatg ccgagcaaaa agaatcatat taaggagagt gtttaattat
     5341 ggcaaagagc tatgattatc aaagtgcttt cgatattatc ggaccagtta tgatgggacc
     5401 ttcaagttct catacggctg gtgcagtaaa gattgggaat tcagcacgcg caatattggg
     5461 agatattcct aaaaatattg aaatacatta ttatgaatca tttgctcaaa cacaccaagg
     5521 acatggtaca gatgtcgcta ttgtgggtgg agcaatggga tattcaacat ttgataaccg
     5581 aattaaatct tcattagaaa ttgcagctga tgatggtatt aaaatagata tcatcgaaga
     5641 agaaggcgat agtattggtc aacatccaaa ctgtgcttat attaaatcat ttcgtaatga
     5701 tggtcgctac attgaaatta tcggtatttc aatcggtggt ggtacaatta aaattaaagg
     5761 tatttatata aatggactag aagtagattt aaatcatgga ttaccaattt tagttgttga
     5821 cggaaatatg actaaagcac aagtaaatca tttcattaat gatatcaacg atatgaaatt
     5881 agactgtaaa gatgaattta ttatgataga tgaagataaa tgcttggttg taataccatt
     5941 aaataaagca atctcagaat cgacattaaa tacaattaaa gagaaataca gtgacgtaaa
     6001 cgtttcctat ataaattaga gggggaataa acatgtttga ttcaattaga gagactatag
     6061 attatgccgt agaaaataat atgtcatttg cggatatcat ggttaaagaa gaaatggaat
     6121 taagcggtaa atcacgtgat gaagtgcgag cgcaaatgaa acaaaattta gatgtcatgc
     6181 gagacgcagt aataaaaggg acgacaggtg atggcgttga aagtgtaacg ggctacactg
     6241 gtcatgacgc tgctaaacta cgtgattata atgaaacaca tcatgctttg tctggatatg
     6301 aaatgattga tgcagtcaaa ggtgccattg caacaaatga agtcaatgct gcgatgggta
     6361 ttatttgtgc aacgccaaca gctggttcct cgggtaccat tcccggtgca ctttttaaat
     6421 tagaaaaaac acatgattta acagaagagc aaatgattga cttcttattc acttcagcat
     6481 tgtttgggcg tgtcgtagca aacaatgcaa gtgtagctgg tgcaacaggt ggctgccaag
     6541 ctgaagttgg ttcagcatct gcaatggcgg cagctgcagc agtagctata ttcggaggat
     6601 caccagaagc atccgggcac gctatggcat tagcgataag taatttatta ggtttagttt
     6661 gtgacccagt agccggactt gttgaaattc cttgtgttat gagaaatgca attggttcgg
     6721 gtaacgcttt aatttcagca gatttagcat tagcaggtat tgaaagtaga atccctgttg
     6781 atgaagttat tgaagcaatg gataaggttg gtcgtaacct tcctgcatca ttacgtgaaa
     6841 ctggattggg tggactagca ggcacaccaa ctggcgaagc aattaaacgt aaaatctttg
     6901 gcacagctga agatatggtt aaaaataatt aatgagataa caataaaata ttgataaaaa
     6961 agaagcatct gtctggatca ttagtaccag atagatgctt cttaattatc tttcataaag
     7021 ttgaaaaatt tatgggaaat gcatgctaat caaaatagtg attgcaaaag cgcccccgta
     7081 tatctaaaat aacttaaaag gagtgatgat atgctaagac tatcaatcat ctttatagca
     7141 ttcatcatta acacaacgat tacatatgga tatacgactg agggaacgtg ggtcaatcta
     7201 ttatttaaat ctttatcgct cagcatgatt atcgttttca tgttctacta tattcgtttt
     7261 gttattgaga aaaagcgtta aatcaaagct atggggatag tatttgaaaa gaataaataa
     7321 aagggacgat gtaaatcaat gtaa
//
LOCUS       DAMZPP010000139         7342 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMZPP010000139 DAMZPP010000000
VERSION     DAMZPP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.139"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..1362)
                     /locus_tag="POH73_002316"
     CDS             complement(<1..1362)
                     /locus_tag="POH73_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDI6888237.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGILVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHINTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLNLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNNNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISINQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTVEEVLDEVGLLDKVQSFTQGINTIIGEGGEML
                     SGGQ"
     gene            1581..2456
                     /locus_tag="POH73_002317"
     CDS             1581..2456
                     /locus_tag="POH73_002317"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDI6888238.1"
                     /translation="MFIIELIKGIILGIVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNNQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QISYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            2628..3311
                     /locus_tag="POH73_002318"
     CDS             2628..3311
                     /locus_tag="POH73_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888239.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEVKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            3314..3772
                     /locus_tag="POH73_002319"
     CDS             3314..3772
                     /locus_tag="POH73_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDI6888240.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVITQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEKSLLRVIHRIKELD"
     gene            3774..4340
                     /locus_tag="POH73_002320"
     CDS             3774..4340
                     /locus_tag="POH73_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDI6888241.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(4435..4977)
                     /locus_tag="POH73_002321"
     CDS             complement(4435..4977)
                     /locus_tag="POH73_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6888242.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEQHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(5057..5356)
                     /locus_tag="POH73_002322"
     CDS             complement(5057..5356)
                     /locus_tag="POH73_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888243.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGRTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            complement(5494..5883)
                     /locus_tag="POH73_002323"
     CDS             complement(5494..5883)
                     /locus_tag="POH73_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888244.1"
                     /translation="MKKLIISIMAIMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKEASQDKQSKDQ
                     TKSSDKDNHKKPTSTDKDQKANDKHQS"
     gene            complement(5950..6393)
                     /locus_tag="POH73_002324"
     CDS             complement(5950..6393)
                     /locus_tag="POH73_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6888245.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQAESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISSTIHKSNLPMISLNKDLGYQVSHVKMYKDID"
     gene            6520..7209
                     /locus_tag="POH73_002325"
     CDS             6520..7209
                     /locus_tag="POH73_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HDI6888246.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKSGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
BASE COUNT         2484 a         1191 c         1077 g         2590 t
ORIGIN      
        1 ctgtccacca gataacattt cgcctccttc acctattatt gtattaatgc cttgtgtgaa
       61 agattgaact ttgtctaata aacctacttc gtctagcact tcttcaacag tattattttc
      121 tatatcttta aacatcgtaa tattctcttt tatagaagca ctgaaaatat atggctgttg
      181 actaagtatt cctatattta aatcacgctg gtttatactt atagtaccta ttgttggctg
      241 ataaacacct gcaataagat gtgtcaaagt tgatttacct gccccgcttg gacctacaag
      301 tgcaatttga tcacctttaa atatttccaa atttaaatca tttaatacca atctatcaga
      361 atcatcatat cgaaatgata tgtctgttaa ctgaataaat ggtttttgat tttcctcata
      421 ctttaataga aattcattat tattgttcgg ttgttctaaa aactcaaaca caacgtcact
      481 ggcaccttca ctttgttttc cagtatggaa tgcttgtcct aagtccttaa ttgcattata
      541 aaattcaggc gctaaaataa tcgcaattgc cgcagtttta aaatcaatat tatgaaatac
      601 tactaagttt agcgttgctt ccaatgcaac caatccaata cctaacatac ttataaattc
      661 gagcattaat cccgataaaa aagcactgcg taaaatgcgc attgttaaag ttctaaactg
      721 agtactatcg tcgtaaatat gcttctctgt ttgctctgta cgattaaata gctttaacgt
      781 cactaaacct ttagcaatat ttaaaaaccg ttgactaaat tgattcaaat aagtcatttg
      841 atcttttgac tcatctcgcg ttttcaaacc gaaaataata taaaacaaag gaataaatgg
      901 tgcagttatt aacataatta atgcggtatt gatatggatg aaaaacattg caatgattat
      961 gatgagcgga accatcattg atttgaacac ttgaggcaaa taactcttat aaaaaggtgc
     1021 tagaccatca atgttttctg tgagtatagt catttgttca ccgattggat gaccattatt
     1081 tttataaata acccgctgtc taagcatatg cttaacttta aatgctaatg tatcacctaa
     1141 ccattgattt agaaattgca cagttgctct taaaagtaaa acacctaata aaataaataa
     1201 tacaatccat aaaccttgaa attgatgtct tataatttta gctaaaaaat ctgctattaa
     1261 aatattttgc gttataacga gtatgccgag accagtactg accaagaaca tgagtaccgg
     1321 aaaaatttta tattgaaaca gtattgttgt taattttttc acaattatat cacctaaacc
     1381 tattataaaa ggttttcctt aaaatcaaat taaatagctc aataaaatat tgtagtttta
     1441 catgtaaaag aaaatgaata tatacatctt tagtctagta ttataactat aactagcatg
     1501 tctttaaatg aatatgctat catgatatgg taattttgat atttaaataa attaaattga
     1561 gtaatgaaag tgggtacatt atgtttatca ttgaattaat aaaaggtatt atcttaggga
     1621 tcgtcgaagg attaacggaa tttgcccctg tttcctctac tggacatatg attctagttg
     1681 atgatatgtg gttaaaatca tctgaatttt taggttctca atcagcattt acatttaaaa
     1741 tcgtcatcca attaggatcc gtctttgcag cagcatgggt gttccgcgaa cgcttcttag
     1801 agattttaca tattggtaaa cacaaacatg ttgaaggaga taacaatcaa caaagacgtt
     1861 caaagccaag acgtttaaat ttattacatg tattagtggg tatggtgcca gcaggtattt
     1921 taggcttact atttgatgat ttcatcgaag aacatttatt tagtgtgcca actgttatga
     1981 ttggcttatt cgtaggtgct atctatatga ttattgctga taaatactca gctaaagtta
     2041 aaaatccaca aacagtagat caaatcagtt atttccaagc atttgtcatc ggtatttctc
     2101 aagctgtagc tatgtggcct ggtttcagtc gttcaggttc aacgatttca actggggtat
     2161 taatgaaatt aaatcacaaa gctgcatcag actttacatt tattatggct gttccaatta
     2221 tgttagcggc tagtggttta tctttattaa aacattatca agatattcag attgctgata
     2281 taccttttta tattttaggc tttttagctg catttactgt tggactaatt gcaatcaaaa
     2341 cattcttgca tcttattaac aaaatcaaat taattccatt tgccatttac agaattgtat
     2401 tagtaatctt tattgctata ctttacttcg gatttggcat tggtaaaggt atttaatttc
     2461 cttttaaaat atagactgtc aattaaggaa ccacttgatt ggcagttttt attgtcttct
     2521 ttgaaaaaac atctgaaata cttataagtg attcactact ctcatcgttt atattgatgt
     2581 atcatagggt atgtaaaatt aataaagtaa ttatggaggc aagatctatg gataagaaaa
     2641 aagtcatcaa atttatgatt aatgtattac caattgtatt ggtaccgtta attgttgaac
     2701 gtaaacgtat caaacaacat ccggacgtac aaaaagttac agatgctaca agtaaagttg
     2761 cttcaaaaac atctgcagca atcagtaaca cagcgagtga tgttaaagaa tatgtcggcg
     2821 ataaaaaaca agattttgaa aataagcgtg aacttaaaaa gtttgctaga gaacatgatc
     2881 ctgcctatat tgagaaaaaa ggcgaaaaat tagctaaaca aaatcgtaaa gacgctgata
     2941 aaatgaataa aatacttcaa aaaaatatcg aaaagcgtca taaagaagag caaaaagccc
     3001 gcgaaaagaa tgaaatacaa cgtattaaag atatgaaaaa gtcacaaaaa tacgaagtaa
     3061 aagcaggctt aacacctaat aaattagatg agaaaactga gaaaaaaggc gataaactag
     3121 ctgaaaaaaa tcgcaaagaa atcgctaaaa tgaataaaaa gttacaaaaa aatattgaaa
     3181 aacgacacaa agaagaacaa aaacgccaac aagaagctga taaagcacgc atcaagtcat
     3241 ttaaaaaata taaagattat gttgccaaaa gcgcctctca acaaaataaa gaaaacaata
     3301 cagaggcata attgtgacac atattattat tgatggagat gcttgtcctg ttgttgattc
     3361 tattatagat ttaacaactg agacaggcat ttttgtgaca attattcgga gcttcagcca
     3421 tttttcgaac caattatatc ccccacatgt atcaacatta tatgttgatg atggaccaga
     3481 tgcagttgat tacaaaattg ttcaattatc aacgaaagat gatatcgtaa tcactcaaga
     3541 ttatggactc gcaagtttat tagtcgacaa agtcttaatt gtcatgcatc ataatgggaa
     3601 gatatacaat tccaaaaata ttcaacaact attagataaa cgatatatga atgcacaaat
     3661 tagaaaacaa ggtggtcgcc acaaaggtcc cccacctttt acaaagcaag atcaaaaagt
     3721 gtttgaaaaa tcactattaa gagtcattca tcgaattaag gagctggatt aatatgaagc
     3781 gaatcgcagt ttattgtggt gcaagcaaag gtcatgaccc ttcatatgta caaaaagcat
     3841 acgatttagg taaatatttt gctgaacaag gttatgaatt agtatttggt gcagggtcaa
     3901 ttggtattat gggtgccatt caagatggtg tattagatca tggtggcaaa gctattggcg
     3961 tcatgcctaa aatgttagat gaacatgaaa tcacaagcca acgtttaact gaattaattt
     4021 tagttgattc tatgcatgaa cgtaaaaata aaatggcaga acttgcagat gcatttgtca
     4081 tggctcctgg tggcgcaggt tcactagaag aatttttcga aatgtatagt tgggctcaaa
     4141 taggtatcca tgaaaagcct attgccattt ataatattaa tggctttttc aatccactac
     4201 aaacaatgat tgaccatatg attgaggaag gttttattga tcctaagtat cgtgcacttg
     4261 caccgttatg cgacactaag gaatcattaa tagaatcaat attaaatttc aaacctctag
     4321 gtactcgttc atacgattaa tcattctaag gttaaaaaat aaacaaacac ccctaatatt
     4381 gttatcatca gctaaacaat attaggggtg tttgttacgc ttatgtataa attttcactt
     4441 tcgaagatta atgtaatagc gcacttgcct ttttaataaa tgatacttac ttagattggg
     4501 taattcgaaa tgatcataca ctgtcatgcc aattttttcc attacacgtt gtgaagcttt
     4561 atttgcttct gctgtaaaac tatagacatc gtatatttga tgttgttctg ctaacttcaa
     4621 cgttgccttt gcgccttcag ttgctaatcc ttttccccaa aattctggca acaagcgcca
     4681 acctatttca taaagcggta attctttaaa tggataatcg cttgtttctg gaatataatt
     4741 caaacctata aagcctatcc attgatggga ctctttatct tctacagcaa ataatccaat
     4801 gccataatct ttaataaccg catccatagt tctcatatct aattctgaac gacgataact
     4861 caataaactt ggaaaatatt tacgtacgtc ataattcgca ttcatttttt gaaaaggtaa
     4921 cagatcatct tcatgccagt ctcttaatat taaacgctct gtttcacaat aaatcatcga
     4981 ttgacactcc tatataattt ctcaataaaa aacacaaaat cgatattttg atcaactttg
     5041 cgttttagat agcagtttat tttgatttag aaatatagct tataagttca acatgaactg
     5101 gcacttcttt taaacgtgct ctatctgatt tcagcgcagt aaaagttgca tctataaaat
     5161 cttgttgatg gtttaaacga tatgtagcaa caaaagtatc tgtatcggac tcaaagtttg
     5221 gtaaatgtgc aattacacgc ttaatttcgc cagtagaatc agcaatcgtg cgtcctgtaa
     5281 tattttctaa ttctctacct aattctggca aagtaatatc ttgaccctct aaaattctaa
     5341 aatcttgttc atgcatgccg tttctctcct ttattaatta cgttgtctat ttttacgttc
     5401 tgtttcaatc atgaccacat tttaattaat acataccctt aaaataaggt ttaaaacgct
     5461 gtacctttca tctataacaa gtaagcaatt caattatgat tggtgtttgt cattagcttt
     5521 ttgatcttta tctgttgatg taggcttttt gtgattatct ttatccgatg acttagtttg
     5581 atcttttgat tgcttatctt gtgatgcctc tttatctttg tctttatttt tgtcttcgtc
     5641 ttttttatcg tccttgttat cttcttttga aggttttaaa tctttcactt ctttttcaag
     5701 gtctttttgc ttatctgcta atttttcttt ttcttgttga agcttttctt ttttatcttt
     5761 taattcttta ttttcttttt gtaaattatc gatatccttt tccagagtgg ctttctcttg
     5821 gcttttacca caacctgtta aaaatagcat gatcgccata atgctgatga ttaatttctt
     5881 catataaaac tccttaacgt cattaaatct tttttaattt taacatgaat tagcaacata
     5941 atcctaattc taatcaatat ctttatacat ttttacatga ctcacttgat aacctaaatc
     6001 tttattcaaa gatatcattg gtaaattact tttatgaatt gtactgctta ttcgctttgc
     6061 attcatagtt tttgcccatt tttcaagcgc aatcttcagt tgcgtagcta ttcccagttt
     6121 gcgaaattgt ggttcaacat atagcagttc aatgttaacc atactttttt cattactaaa
     6181 atgtccccaa ataaacgcta ttaattgacc ttcattttca tatatataaa ttttatcatt
     6241 tgtatgttct aatcttgaac atatcatctc gtacctaaga gcaatactca gtttagtaca
     6301 tttatagttg gattctgctt gcgacaataa tgtctcatgt atattagcta tttgcttgat
     6361 ataattatgt tcatctttat taagtgtcct cattttcttc tccgatatta atcgttatat
     6421 ttatgcgttt aatatgatat gattgacctt attaatacta taaaatcatt tccattttag
     6481 tttggaatta aacattttta aaggagcgct ttagtaacaa tgaaaagtac tgcgcaatta
     6541 acaaaagaaa ataatgttaa atcattaaga ttaagcaata ctgatagaga aatcttcgag
     6601 aattatatga cttatatgcg ctctgacttt cgcgtcaatc ctcatgatac agagttaatt
     6661 attaaccgta tacttaaaca attattaagc gcagaacaac atggtttatt agccttagat
     6721 tttttcaatc acgacccaaa agcacatgca ataaaagaac taaaagcgat gcctaatgaa
     6781 acatttaaaa atatttttaa atatatatat cagcacatcg ttctattaat tggtattgtt
     6841 agttttctca aaggattttt aggatttttc atggaaaaaa gtggaagtaa tttatatttt
     6901 gtttcattcc cgttttcagt agtcgttgga ttctttattg tctttttatt tatatggttt
     6961 agttttaaaa caattcaact gcagtgtttt aacaattcaa attggatatg gatattcacc
     7021 tatttagcaa tcattctact aattgtagga tttttctatg tattctttat tccacaatca
     7081 ttacttgcct ttggaccata tattcaggta agcaattggg tatttattat attttcattt
     7141 atagttatgc ctattggatt acgcattgaa agaaatattt cgaaaaaaca ttctcataca
     7201 tttttataaa caaaaagctt attacatcat atgctaattt ggtactgtta atgataaaat
     7261 cactaaatta cagttaccta aaaacatatt ttgcaataag ctttttatta attaatcaat
     7321 aaagcatcta taaagaacca ta
//
LOCUS       DAMZPP010000140         7222 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMZPP010000140 DAMZPP010000000
VERSION     DAMZPP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.140"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(17..2086)
                     /gene="eap"
                     /locus_tag="POH73_002326"
                     /gene_synonym="map"
     CDS             complement(17..2086)
                     /gene="eap"
                     /locus_tag="POH73_002326"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HDI6888247.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNTNEASAAAKQIDKSSSSLHH
                     GYSKIQIPYTITVNGTSQNILSSLTFNKNQQISYKDIENKVKSVLYFNRGISDIDLRL
                     SKQAKYTVHFKNGTKRVVDLKSGIHTADLINTSDIKAISVNVDTKKQVKDKEAKANVQ
                     VPYTITVNGTSQNILSNLTFKKNQQISYKDLENNVKSVLKSNRGITDVDLRLSKQAKF
                     TVNFKNGTKKVIDLKAGIYTANLINTGDIKNININVETKKQAKDKEAKANNQVPYSIN
                     LNGTTTNIQSNLAFSNKPWTNYKNLTAKVKSVLKSDRGVSERDLKHAKKAYYTVYFKN
                     GGKRVIHLNSNIYTANLVHAKDIKKIEVTVKTGSKANAERYVPYTIAVNGTSTPNLSD
                     LKFKGDSRVSYSDITKKVKSVLKYDRGIGERELKYAKKATYTVHFKNGTKKVINLNSK
                     ISQLNLLFVKDIKKIDVDVKTGSKAKADSYVPYTIAVNGTSTPIASKLKLSNKQLIGY
                     QDLNKKVKSVLKHDRGINDIELKFAKQAKYTVHFKNGKTQVVDLKSDIFTRNLFSVKD
                     IKKIDIDVKQHTKSNKALNKVANIATKVKFPVTINGFSNVVSNEFAFLHPHKITTNDL
                     NAKLRLALASDQGITKHDIGLSERTVYKVYFKDGSSKFVDLKAAKQDSKVFKATDIKK
                     VDIEIKF"
     gene            2472..2722
                     /locus_tag="POH73_002327"
                     /pseudo
     CDS             2472..2722
                     /locus_tag="POH73_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187510.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            3044..3223
                     /locus_tag="POH73_002328"
     CDS             3044..3223
                     /locus_tag="POH73_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888248.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNRESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
     gene            3534..3794
                     /locus_tag="POH73_002329"
     CDS             3534..3794
                     /locus_tag="POH73_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888249.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDVVHLTIFILNLLFSILYCLPILFYL"
     gene            complement(3847..4197)
                     /gene="scn"
                     /locus_tag="POH73_002330"
     CDS             complement(3847..4197)
                     /gene="scn"
                     /locus_tag="POH73_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HDI6888250.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
     gene            4881..5330
                     /locus_tag="POH73_002331"
     CDS             4881..5330
                     /locus_tag="POH73_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-inhibiting protein CHIPS"
                     /protein_id="HDI6888251.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTV
                     YLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY"
     gene            complement(5425..5760)
                     /locus_tag="POH73_002332"
                     /pseudo
     CDS             complement(5425..5760)
                     /locus_tag="POH73_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(6410..6901)
                     /gene="sak"
                     /locus_tag="POH73_002333"
     CDS             complement(6410..6901)
                     /gene="sak"
                     /locus_tag="POH73_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HDI6888252.1"
                     /translation="MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVII
                     EKK"
     gene            complement(7092..>7222)
                     /locus_tag="POH73_002334"
     CDS             complement(7092..>7222)
                     /locus_tag="POH73_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6888253.1"
                     /translation="QAVYAPGTLIYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
BASE COUNT         2443 a         1088 c          941 g         2750 t
ORIGIN      
        1 aatctaaaat taaagattaa aatttaattt caatgtctac ttttttaatg tcagttgctt
       61 taaatacttt tgaatcttgt ttcgcagctt ttaagtcaac aaattttgat gatccgtctt
      121 taaaatacac tttatacaca gtgcgttcag aaagtccaat atcatgttta gtaatacctt
      181 gatcgcttgc taacgctagt ctaagtttag cattcaagtc gtttgttgtt attttatgtg
      241 gatgtaaaaa cgcaaattca tttgaaacta catttgaaaa tccatttatc gttactggaa
      301 acttcacttt agtcgctatg ttagccactt tattaagcgc tttattagat ttagtgtgtt
      361 gttttacatc aatatctatc tttttaatat ctttgacact gaataagttt cttgtaaaga
      421 tatctgattt aaggtcaact acttgtgtct ttccattttt aaagtgtaca gtatactttg
      481 cttgtttcgc aaatttcaat tcaatatcat tgatacctct atcatgtttt aaaactgatt
      541 taactttttt atttaaatct tgataaccaa ttaattgttt attcgaaagt tttagttttg
      601 atgcaatagg tgttgatgtc ccatttactg cgattgtgta tggtacatag ctatccgctt
      661 tcgcttttga ccctgtttta acatcgacat ctatcttttt aatatctttg acaaaaagca
      721 aattcagttg gctaattttt gagtttaaat taatcacttt ttttgttcca tttttaaaat
      781 gtactgtata agttgctttt ttagcatatt ttaattcacg ttcaccgata cctctatcat
      841 acttcaatac tgatttaact tttttagtga tatcactgta acttacacgt gagtcacctt
      901 taaattttaa atcagataaa tttggtgttg atgtcccatt tactgcaatt gtgtatggta
      961 cataacgctc tgcattcgct tttgatccag ttttaactgt aacttcaatt ttcttaatat
     1021 ctttcgcatg aactaaatta gctgtataaa tattcgagtt taaatgtatc actcttttac
     1081 caccattttt aaagtaaaca gtgtaatacg ctttctttgc gtgtttcaaa tcacgttcac
     1141 taacgcctct gtcagatttc aatactgatt taacctttgc tgttaaattt ttgtaatttg
     1201 tccaaggttt atttgaaaat gctaaattag attgaatatt agttgttgta ccatttaaat
     1261 taattgaata tggcacttga ttatttgctt ttgcttcttt atctttcgct tgctttttag
     1321 tttctacatt tatattgata tttttaatat cgcctgtatt gattaagttc gctgtataaa
     1381 tgcctgcttt caaatcgata acttttttcg tgccattttt aaaattaact gtaaatttcg
     1441 cttgttttga aagtcttaaa tctacatcag ttatacctct gtttgatttt aaaactgatt
     1501 ttacattatt ctctaaatct ttataactaa tttgttgatt ctttttaaat gttaagtttg
     1561 ataaaatgtt ttggcttgta ccattcacag tgattgtata cggcacttga acatttgctt
     1621 ttgcctcttt atctttcact tgctttttag tatctacgtt aacactaatt gctttaatgt
     1681 cacttgtatt aattaagtca gctgtgtgaa tgcctgattt caaatcgaca actctttttg
     1741 tgccattctt aaaatgaacc gtgtattttg cttgcttaga aagtcttaaa tcgatatcac
     1801 taatacctct attaaagtac aaaactgatt taactttatt ctctatatct ttataactaa
     1861 tttgttgatt cttattaaat gttaagcttg ataaaatgtt ttggcttgta ccattcacag
     1921 tgattgtata tggaatctgt attttagaat atccatgatg taatgaactt gatgatttat
     1981 ctatttgctt agctgcggca gatgcttcgt tagtattaaa gtttgctcct gttgatgcta
     2041 aaacacctaa tgctaatgtt gttgtaatca atgacttaaa tttcataaat tatctctcct
     2101 tttttatgta attcgtgatt gcaacttaat tatagccaga ctttctctat tttttgaatt
     2161 aactgaatat taataataaa ttatctttaa caataatttt ttaacactgt taaaagttct
     2221 tttaattttg attaactaat taatttacaa tacctaaaat gttgtttgat tttgtttata
     2281 ccaagcttca aacttaaatg tcattacaac cttcatttct taaatccgat tacatttgtc
     2341 aattatattt acagcatctt tatactcaaa aaacatttac tcaaaaatat aaattcgatt
     2401 taataattaa tttaaattta gttaatcaat tttgcatcta ttttgttgta agctatataa
     2461 aaggagtgat aatgatggtg aaaaaaacaa aatccaacac actaaaaaaa gatgcaacac
     2521 ttgcattagc aaatttatta ttaattgggg cactgacaga caatagtgcc aaagccgaat
     2581 ctaagaaaga tgatactgat ttgaagttag ttagtcataa cgtttatatg ttatcgaccg
     2641 ttttgtatcc aaactggaga cttttaacat aaaattactt atcattcaaa aagtaaaaca
     2701 gcataatatc aaggtttata actttatcat tatcaataat acctcatata aaataaaatt
     2761 ttagggactt tttagggact ttaaatttaa aattacaagt ttaatagaaa catcaaaata
     2821 atcacatgtt tgtgtggaat gtacacctca aaagctagac tgaaaaatct attttttgag
     2881 gtgtattttt ataggtaaat ataataaatt agagtagaca actcagaatt ccaattttat
     2941 aataatattg cttgacatat caaactagat agtactattt tgaatatatt attataatca
     3001 aaaattcatt gtaaactttt agacaaaagg aagtaataaa aacgtgaaat ttaaaacaac
     3061 taaagaatgt aaaagtaata atatctttaa aagaagtcaa gaaattaata atagagaaag
     3121 tgaaaagggt tgtttatggg gcattagcat gttgattcta cttttcttat tgattctgtt
     3181 tggaataact gcttgttcat caagcattca ctttattaat tagatttttt tacttggagg
     3241 tatcatgtga agaaccatac aaatataatt aatatcttat tagttatagt caactcatta
     3301 actcattttc taactctaaa cacctcattt tttaataatt cagcatcgga tttctgtttt
     3361 atcatagggg ctatattttt cttaatcgga atttttgttg caatatacgg tatgaagcga
     3421 gcaacatatt ggttaaactt attgatttta tttaccaata ttttttattt tctacacttc
     3481 tgtgtgttac ttttgttaaa atatatagga tttaaattat ttatttatga agggtgtgta
     3541 ttgttattta tctaatttat agtctaattg tctatttcat ctgcattata aattttttca
     3601 taatggctgc accattctta atgactaatg ctgattatgt atggacgcct atgactacag
     3661 ttacgttatt tattttgagt ttgattattt tcttgatatt tataaaaaca aaagatgtcg
     3721 ttcatttaac aattttcata ttaaacttac ttttttcaat actttattgt ttgcctatac
     3781 tgttttattt atgaacactt ttatattatc tagaaaatta aaaccacccg taaaagggtg
     3841 gtttttttaa tatttacttt ttagtgcttc gtcaatttcg ttataaatct tttgaagttg
     3901 atattttgct tctgacattt tcttaaagtc ttttgactta agagcataca ttgctttttg
     3961 acctgaaatt tttatagttc gcttataata agtgtttaaa cttccagtag ctaattcatt
     4021 aacatttagt tcatctaata acgattttaa ttcattagct aacttttcgt tttgatattc
     4081 attcgatgtt ggcaagcttg tgctagcttg tgcctcattt ttatctagat tagttactag
     4141 tggtgatgct aaaacgattg ctaaagttcc cgcaagtata gattttctaa ttttcatttt
     4201 ttattctcct tagtaattag tactaattta ttttaatgac taactaaaaa aagtacatta
     4261 attattcatt aactatatag atcttcttat taaatgaaaa ttaataaatt atcaaattga
     4321 cataccttat tgtatttata gaaaaacaaa aaaaggtaag caccgaaatg cttacctact
     4381 tcccataaac aatataacac atatacattg atttggaaag cgcaaaaata aatttaatat
     4441 actagcccga aggggagcaa tacataaaaa atgaaaggcg ctccttgaaa acgcccaagg
     4501 taatattaac ataaaatggc tactattgca ttatctaatt ttatttaatt aaaacaaata
     4561 tatatagcac aaaaaaacta gcccgaaggc tagcttatga atagatgaaa atttgaacac
     4621 attgctgtgt ctaagataaa tatagcacat attaattact attaatagaa aaattatagc
     4681 gttttttaga ttatttcaat ttatcgagac caaattcatc aaaacactaa ttgaatttta
     4741 atttttagtt aaaaataatt aatcttttat taatttcaag ttaattaata tatttcgtcg
     4801 attagattaa actcatttta ggaatttcgc aaaactaact ataaatttaa atatagaatt
     4861 taaggagaat taacatcatt atgaaaaaga aattagcaac aacagtttta gcattaagtt
     4921 ttttaacggc aggaatcagt acacaccatc attcagcgaa agcttttact tttgaaccgt
     4981 ttcctacaaa tgaagaaata gaatcaaata agaaaatgtt agagaaagaa aaagcttata
     5041 aagaatcatt taaaaatagt ggtcttccta caacgctagg aaaattagat gaacgtttga
     5101 gaaattattt aaagaaaggc acaaaaaatt ctgctcaatt tgaaaaaatg gttattttaa
     5161 ctgaaaataa aggttactat acagtatatc tgaatacacc acttgctgaa gatagaaaaa
     5221 atgttgagtt actaggtaaa atgtataaaa catacttctt taaaaaagga gagtctaaat
     5281 catcttatgt aattaatggt cctggaaaaa ctaatgaata tgcatactaa tagtagttac
     5341 ataaattaaa aggtagatat ttctttttta tataaaggtt tggcagacat ttcataactt
     5401 gccaaacctt tatatatcta attatcaaac tgcactaaac ttaccaaaac tgcttattct
     5461 attacctgcc ttgtctacct ctcctgtcgc tatataacga cgttgtccac tattagcaat
     5521 ataagtaatc catctatagc cattaatgca atatgcgccg tcatatttga tagttgcgtt
     5581 attgggtaat acacctgtaa ttcttgaatt agttgaatag ccatccctca cgttattacc
     5641 tttaacattg gcaactgtgt aataaccagt ctctttttta tacggtacat tgtttttatc
     5701 gagtgtataa cctgctggca ctggtgaatt cttttcattt ttagctggtg ttttaacatt
     5761 actgatacct gatacacact tccaataaaa ataaccacac catttaagat gcggtgtagc
     5821 gactgtaata tttctatgtt gttgagatat atgtatcgag tgaagggcaa aaaggatatc
     5881 aattgcagga taagtgttag gttactaggc cacttaacag gctatatagt tcactcctac
     5941 tatatacagt taattataac ataaaaagca ccccgtaaac tattatacgg gaatgctaaa
     6001 gtcatatata ctacgaggga gtagtatgaa aactatgctc tctatcataa gaaaaaacac
     6061 ccagtgacat gcttgggtga acaaggatag atgtaaatag ttgatgcatg tgtaacacat
     6121 cataacaaaa aactagcccg aaagctagct ataacataca atctaaaaag acgtcccttg
     6181 aatacgtcta gaaagattat aacataaaaa aataggcaag taccgaagta cctgcctaaa
     6241 aaaggattat ccactttttc atcctaactg atttctcccc ataagtcacc taatatctga
     6301 ttaggtgggg aagaaccatt cgtgcatgaa tgagaatttg atgaaagata attttcacta
     6361 cacatattca atcaagacat tgctttctat aataaacaac tattttgttt tatttctttt
     6421 ctataataac ctttgtaatt aagttgaatc cagggttttt aatatgctct gataaatctg
     6481 ggacaacaaa acctttttct gttataggga aagacttcgt ttcttctttt ttcttattct
     6541 tatcataata agtgacttcg atctttgcgc ttggatctaa ttcaactact ctaaactctt
     6601 tatatgctgt cgcatctaat gcccattcga catagtattc aattttttct tttgtaagtg
     6661 tagtcccagg tttaatagga aactcgacat aatgagggga tagcaattca tttcctttac
     6721 catcaactcc agtcacattt accatcaaat acgggcctgt tggttcaaaa taactcgcgt
     6781 catcgccttt tttatatttt cctttgtcga atgaacttga tgcacttacc tcattagtaa
     6841 ttgaagaaaa tgagaataat aacaataaaa cagttaaaaa taataaacct cttttgagca
     6901 tggcgcttcc tccaaatata atatatttgt ttttatttat ctctttttat agctaacacc
     6961 cttatttaag atgataaata tcaacaactt cattttatat tgagaaaata ttaaaaatca
     7021 ataaaatatt aaaataaact aaaattacat taaaaaataa ttaacaaata tttaacattt
     7081 taacctaaga attaaaacac ttctttcaca atcaatctct catgccatat ccactcatta
     7141 tgattgttcc aataaatgcg acaccaacca tctataattt caaacacata tattaatgtt
     7201 ccaggcgcgt atacagcctg tc
//
LOCUS       DAMZPP010000141         7110 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMZPP010000141 DAMZPP010000000
VERSION     DAMZPP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.141"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..240
                     /locus_tag="POH73_002335"
     CDS             <1..240
                     /locus_tag="POH73_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HDI6888254.1"
                     /translation="PSETNAYNVTTHANGQVSYGARPTQNKPSETNAYNVTTHANGQV
                     SYGARPTQNKPSKTNAYNVTTHADGTATYGPRVTK"
     gene            complement(422..529)
                     /locus_tag="POH73_002336"
     CDS             complement(422..529)
                     /locus_tag="POH73_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888255.1"
                     /translation="MCVRTRLVSSSSVRLSKAIIIAVIVVCHIDVRGLF"
     gene            complement(827..2011)
                     /locus_tag="POH73_002337"
     CDS             complement(827..2011)
                     /locus_tag="POH73_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDI6888256.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRTGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNVGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GYKAVGVDPKIMGIGPAYVIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            complement(2041..4302)
                     /locus_tag="POH73_002338"
     CDS             complement(2041..4302)
                     /locus_tag="POH73_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HDI6888257.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     LVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKLSIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLYENGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDVIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPVSHFVKDELWDKGDAKLSVTHNDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENEHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRHGHFISDHDYHIALNIATILSGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            complement(4489..5700)
                     /locus_tag="POH73_002339"
     CDS             complement(4489..5700)
                     /locus_tag="POH73_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDI6888258.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            complement(5812..>7110)
                     /locus_tag="POH73_002340"
     CDS             complement(5812..>7110)
                     /locus_tag="POH73_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HDI6888259.1"
                     /translation="FACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKLLFYAEKHLSSL
                     TDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSGTTGSPKGVMFS
                     YESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGGTLILQRYFNGE
                     ELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFKAMDYIIQGGSQPLPSIQAAFKQ
                     YGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDNGEEVPTGEIGE
                     LAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDDDGDIFIIDRKKELIITGGEN
                     VLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYAEILDQHMRSRL
                     AGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
BASE COUNT         2155 a         1459 c         1215 g         2281 t
ORIGIN      
        1 ccaagcgaaa caaacgcata taacgtaaca acacatgcaa acggccaagt atcatacgga
       61 gctcgtccga cacaaaacaa gccaagcgaa acgaacgcat ataacgtaac aacacatgca
      121 aacggtcaag tgtcatacgg agctcgccca acacaaaaca agccaagtaa aacaaatgca
      181 tacaatgtaa caacacatgc agatggtact gcgacatatg gtcctagagt aacaaaataa
      241 gtttataact ctatccatag acatacagtc aatacaaaac attatgtatc tttacaacag
      301 taatcatgca ttctatgatg cttctaactg aattaaagca tcgaacaatc ggaagcatat
      361 ttctaaatta tttattcatt atagtcttaa acataacatg acctaatata ttactaacct
      421 attaaaataa accacgcaca tctatgtgac atacgacaat cacagcaata ataattgctt
      481 tagaaagtcg taccgaactg gaacttacaa gtctagttcg aacacacact gatgtgagtg
      541 gttttcttta ttttaaacat gaacaatcag ataagttact agtagtagca aatattatta
      601 aatcaaaggg cttcgattca taaaatttaa aacaatgatt aaaattagac gtgtaaatgt
      661 taaattctaa aacggaaata cccgctatcc tattaaacca ctttttggtt caatcactat
      721 atttcacaca gtttcaataa taaaacgaat ctgcttcaat ccgcttcaac ttcagcctac
      781 ttcattcaat aacaaaacga atccgcttca ttcaaaatca accattctaa cgcacatact
      841 caaatatagc agctgcaccc ataccgacac caatacacat cgtaaccatg ccgtaacgac
      901 tatcgggacg tctacccatt tcattaagta aacgcgcggt taacattgcg cctgtagcac
      961 ctaatggatg acctaaagca atagcgccac cattcacatt cgtacgtgat atatctagac
     1021 ctacttcttt aatagatgca atcgtttgag aagcaaatgc ttcgttcaat tcgattaaat
     1081 caatgtcttc aacagataga ttgctgagtg acaatacttc aggaatcaca tatgcaggcc
     1141 caatacccat aattttcggg tcaacgccta ctgccttata accaacgaat cgtgcaatag
     1201 gtgtcacgcc tagttctttc actttatctc cagacattaa aactacaaat cctgcaccat
     1261 cagaaagtgg ggcagatgtt cccgcagtca cagtgccgtc agctttaaat actgtacgta
     1321 atttggctaa tgcctccatc gtggtgtcag gtcgtataaa ttcatcttgg tcaaagatat
     1381 ttgtgtgtac ttttggtcct acgtttgtat attcaactga gtttacttgt attggaataa
     1441 tttcatcttt gaaccgacca tcacgttgtg cgtcataggc acgctgatga cttctgacag
     1501 cataagcatc ttgatcttcg cgtgatacgt caaattggga tgctacattt tcagcagtta
     1561 aacccatagg atatgacgca cctatatcat catattgtaa ggtaggattg tttgtgggct
     1621 cgttgccacc cattggtacg gcactcatca attcaacgcc accagctaca agtatatctc
     1681 cttgacccgc cataatttga ttggctgcaa tcgcgatggt ttgtaatcct gatgagcagt
     1741 agcgattcac tgtttgaccc ggtacagtgt cagataatcc cgtacgcaat gcaatcgttc
     1801 gtgcaatgtt ttgtccttgt aatccttctg gaaaagccgt accaacaatg acatcttcaa
     1861 tcatattctt attgaatttt ccgtcaatac gtttcaatac gccttgtaat actttggctg
     1921 cgacatcatc aggtctttcg tggaataatg cgccttgctt tgctttcgct gcggctgaac
     1981 gcccataagc tacaatgtat gcttcttgca tggttatcat cctctcttaa tgactatctt
     2041 ttaattacgt aatggcttac cagtttttaa catatgcgca attctttcat atgatttttt
     2101 agattttagt aagtcaataa agccaatttt ctccaacgat tgaatgtaac gttgattgat
     2161 aaatgtattt cttggtaaat caccacccga taaaattgtg gcgatattta aggcgatatg
     2221 ataatcatgg tcgctaataa aatgaccgtg tctttgcgca tctaattgtc cttggatcaa
     2281 tgctttgaag tcttcgccta aagcgatata ttgatgtcta ggattcggaa tatagtttgt
     2341 ttctgcttca tatttcgcac gtttgagcgc aacttcgaca cgttgtgctg tattgaaaat
     2401 aatcgtatct gtatcacgta aataaccata acgacgtgcc tcaaaggcat ttgtagagac
     2461 tttcgcaaat gcgatattcg tcagtacttt tgtcatggaa gcttgtttgt catcaaactt
     2521 atgcgatgtg cgtaatatgc gatcagccat ttctgcaagg ccacctccac tcggtaataa
     2581 gccaacacct gcttcaacaa gaccgatata agtttcactt gcagcgacaa caataggtga
     2641 gtaaagtaca agctcacaac caccgcctaa ggcacgacct tgaacagctg tgactactgg
     2701 tttcaaacta tacttcaaac gattaaagct ataatgtaat ttatcaattg attgtgcaac
     2761 gacatcatct acaagaccgt cttcatgcgc ctttttcatt aagaaaaggt tagcgcccac
     2821 actgaaatta ttaccatctg cataaataac catacttgtg taatgttcat tttccagtaa
     2881 atcaatcgca tcaactaacg catcgttgaa ttcatcggta atgacattat ttttactttg
     2941 taatttcagt aacagttgat cattatgagt tacggaaagt ttggcatcac ctttatccca
     3001 aagttcatct tttacgaagt gagaaacagg cgttgcatat tcaatggtct catcttgttt
     3061 ataaaagcca ccgtctaaat cactaatcca ttgtggtaag tctccaagtt cgtcttccat
     3121 acgtgtttta acacgttcat atcccattgc atcccataat tggaatggac caagtttcca
     3181 gttgaacccc cagacaagcg cacggtctat gtctcggaaa tcatcggtag ctttaggtac
     3241 attgatagca gagtaataga aattattacg taatgtctcc cataaaaata gtcccgcttc
     3301 gtcttgcgca ttgaatatga catcaaggtt atgcactaag tctttattaa attcatttaa
     3361 aattggtaat tgtggttgcg atacaggtac ataatcttgt ttttcaacat cgtaaacaag
     3421 tcgagcttta gtttctttat cctttttgta aaatccttgt tttgttttac gtccgagtgc
     3481 gccattttca tacaacgtat ttacaatttt gacatcatga aaataaggtg tttcttcagg
     3541 tacttgttgc atgcctttaa ttacagacac tgcaatatct aaaccgacta ggtcagatag
     3601 ggcatatgta cctgttttag gacgaccaat cgcttgccca gttaaagcat ccacatctac
     3661 aatgcttaac ttgtgttgct cggcgcgata cataatatca ttcattgttt gcgtgccgac
     3721 tctatttgcg acaaagccag gcacatcatt gacaacaatg acacctttac ctaacacatt
     3781 ttgcgcgaaa ttttttacat ctaatataat agattccttc gtgtgtgacg taggtattaa
     3841 ctccactaat ttcataatac gtggtgggtt aaagaaatgt agaccaaaga atcgttcttg
     3901 atccttctcg ttaaatgctt gagcaatcgc attaattgga atacctgatg tatttgtagc
     3961 gaataaagca tcttctttag catgttgtag aactagttgc caaacagcat gcttaatttc
     4021 aatatcttct ttgactgctt cgatatataa atcagcatca tcatttacca agtcatcatc
     4081 aaaattacca tatgttaaat gactcgctag atttaagtcg aatagtagcg gtcgtttctt
     4141 atctgtaatt ttatcgtaag attttttcgc aatgagattt ggatcgtttt tgtccactac
     4201 aatatctaat agttttactt taagtccagc attcacaaag agtgctgcca gttgagcgcc
     4261 cattgtgcct gcgccaagaa cggttacttt attaattgtc atagtgattc ctccaattta
     4321 gttgaggata agataaccat taagataatt ggaataacgt tgctatttta taaaattaat
     4381 taagtatctt tgacagtcat cttagcctct tatttaagga aaaagcttta tgcttaaaat
     4441 aagtcttttt ttagtggaat tactgcatca catataattc tttgctattt atacgaaagc
     4501 agaatctcca gtcaaagcgc gtccaattac taaggcatta atttcatgtg tgccttcgta
     4561 tgtgtaaatc gcttctgcat cagagaagaa acgtgcaata tcataatcgt cagctagtat
     4621 gccattacca cctgtaatac cacggcccat agctactgtt tcacgcaaac gtaaggcatt
     4681 catcatcttc gcggttgaag ttgcaacctc atcatattca ccatgtgctt gcatattagc
     4741 taattgagca catgttgcca ttgcttgagc taaattacct tgcatcattg ctagcttttc
     4801 ttgtattaac tgatatttac taattggttt gccgaattgc ttacgctcag tgacataatc
     4861 taatgtggca cgtaaagcgc cagccatacc acctgtagcc atataagcaa cgcctgctct
     4921 tgttgaataa agaattttgg caatatcttt aaagcttgtt atgttttgta agcgatccgc
     4981 ttcatctact ttgacattag ttaatttaat tagggcgtta ggaacaatgc gaagtgcgat
     5041 tttattatca atgacttcaa tatcgacgcc atcttgttct ggtctgacta caaagcaatg
     5101 gggtttgcca gtttctttat ttactgcgaa tactggaatg acatcagata catgtgcacc
     5161 accaatccat ttcttttcac cattgataac ccaagtatcg ccctggcgtt cagcgactgt
     5221 ttcaagacct cccgcaacgt ccgaaccgtg ttctggttca gttaaagcaa agcatgtacg
     5281 cagttcatgt gactgtaatt taggtacata tttcgcaatt tgttctttgc tacctccgaa
     5341 atagaaagtg ttatgcccta aaccttggtg aacaccgagt agggtagcta aggaaatatc
     5401 aaatcgcgcg agtaggtaag acatgaaaaa ctgaaatagt tgactaggca ttttggcgtt
     5461 tggacgatcc ttgtatagta atggattgtt aaaataattt aattctccca gatctttaaa
     5521 atagtcctcg ggtacagtag cgtctatcca atgttgatta atattttcac ggtacttact
     5581 ttctagcaat gaatctactt gttgtaaaaa ttcgacttca ccgtctgtta aacctttagc
     5641 aatactaagt acatcttcag gaaataatgt ttttaagact gtttcttttt caaatgtcat
     5701 ataaattcct cctaaaaata atatgaatac taatgtgaaa tgcatttaat tcaaaacaac
     5761 acgctttatt tgtaaacgct tacactaaat gctaaaaatt ttttatcacc tttaaagtgt
     5821 ttgtgagact ttgtcattca tcatttgtcg aatcgcaagt ttatctggtt tctgcgtact
     5881 gtttaacggc atatgtgtca ctggtacata cattcttgga actttataac ctgctaaacg
     5941 acttcgcata tgttgatcta aaatttcagc gtaatgaggt tcatcttcac gaagtataat
     6001 ggctgcagca attgactcac catattttgg atgatcatag ccaacgacca cacaccggtc
     6061 tactagtgga tgctccgcta aagcattttc gacttcggat ggtaagacat tttcgccacc
     6121 agttatgatt aattcttttt tgcggtcaat aataaatata tcgccatcgt cgtccatctt
     6181 cgctaaatct cctgtcaata agtaacgacc atgaaacgct ttagcagtct ctgctggttt
     6241 attccaatat cctggcgtga catttttagc cttaattgca agttcgccaa tctcaccagt
     6301 aggtacttct tcaccgttat catcaaggat acgtgcatca acaaacatga ctgctttacc
     6361 aatactcatt ggtttacgtt ttgaattttc cggtgtatta acaagtacaa gcggtgcttc
     6421 agttaagcca tagccattaa taatgtttat gccatattgc ttaaaagctg cttggatact
     6481 tggtaatggt tgtgaaccac cttggatgat ataatccata gctttaaaat tttcaggatt
     6541 aaaattacta gcacgtagcg tactataata cattgtcgga atcatgataa taaatgtagg
     6601 gtgatattgt gcaatcatgt cattcaattc ttcgccgtta aagtaacgtt gaagaataag
     6661 tgttccacct gacattaata ctggtaatac agtatcgtta aaccctaaaa catggaacat
     6721 tggtgttgat acaatcgtaa tatagtttga attgaacttg tacgttagct ctaagtttgc
     6781 gccgttatga acaaatgatt catatgagaa catcacacct ttaggtgatc cggttgtacc
     6841 acttgtataa attaatgctg caagatcttg tggttcaaca ggtgttgctt gaaaaggttg
     6901 gtgataatct ggatttacga tttcatcata ttgcgctaca tcaatatcca tatgcaataa
     6961 gttttggtca atatcggtga gtgaacttaa atgtttttca gcatagaaga gcagttttaa
     7021 ttgtgcatct tccacaatgg ctgcaatttc ttttgggtta agccgccaat tcaatggtaa
     7081 aaaaaccgca cctgttttaa aacaagcaaa
//
LOCUS       DAMZPP010000142         6821 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMZPP010000142 DAMZPP010000000
VERSION     DAMZPP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.142"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..1189
                     /locus_tag="POH73_002341"
     CDS             <1..1189
                     /locus_tag="POH73_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDI6888260.1"
                     /translation="HTITIVGLGNYGIDDLPLGIYKFLMTQDKVYARTLDHPVIESLQ
                     DELTFQSFDHVYEAHDQFEDVYNDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKLLA
                     LAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLITQV
                     YSSMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLTSV
                     FVPKITTPTYLYHDFDFATEVIDTLVDENKGCPRDKVQTHETLKRYLLEETFELFEAI
                     DNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDANA
                     ETIDDLKEIWSKAKDAEGKQPRVKFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEKGE
                     SNT"
     gene            1186..1449
                     /locus_tag="POH73_002342"
     CDS             1186..1449
                     /locus_tag="POH73_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDI6888261.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            1467..1859
                     /locus_tag="POH73_002343"
     CDS             1467..1859
                     /locus_tag="POH73_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDI6888262.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            1964..2365
                     /locus_tag="POH73_002344"
     CDS             1964..2365
                     /locus_tag="POH73_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDI6888263.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            2545..3840
                     /gene="tilS"
                     /locus_tag="POH73_002345"
     CDS             2545..3840
                     /gene="tilS"
                     /locus_tag="POH73_002345"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDI6888264.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEESRFLEAYCERHHIDLHIKKLDLSHSLNRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRNGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIEK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVQQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKQWIIISKNGDE"
     gene            3845..4384
                     /gene="hpt"
                     /locus_tag="POH73_002346"
     CDS             3845..4384
                     /gene="hpt"
                     /locus_tag="POH73_002346"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDI6888265.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            4642..6735
                     /gene="ftsH"
                     /locus_tag="POH73_002347"
     CDS             4642..6735
                     /gene="ftsH"
                     /locus_tag="POH73_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDI6888266.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHRQA
                     PNIEKPYDPNHPDNK"
BASE COUNT         2526 a          912 c         1358 g         2025 t
ORIGIN      
        1 acataccatt acgattgttg gcttaggaaa ctatggcatt gatgatttgc cgctagggat
       61 atataaattt ttaatgacac aagataaagt ttatgcaaga acgttagatc atccagttat
      121 agaatcgttg caagatgaat taacatttca gagttttgac catgtttatg aagcacatga
      181 ccaatttgaa gatgtctata atgatattgt ggcgcaattg gttgaagctg ctaatgaaaa
      241 agatattgtc tatgcggttc cgggtcatcc tagagttgct gagacaacta cagtgaaatt
      301 actggcttta gcaaaggaca atactgatat agatgtgaaa gttttaggtg ggaaaagctt
      361 tattgatgat gtgtttgaag cagttaatgt agatccaaat gatggcttca cactgttaga
      421 tgcgacatca ttacaagaag taacacttaa tgttagaacg catacattga ttacgcaagt
      481 ttatagttca atggttgctg ctaatttgaa aatcacttta atggaacgat atcctgatga
      541 ttatcctgtt caaattgtca ctggtgcacg aagcgatggt gcggataacg ttgtgacatg
      601 cccattatat gaattggatc atgatgaaaa tgcattcaat aatttgacga gtgtattcgt
      661 accaaaaatc acaacaccga catatttgta tcatgacttt gattttgcaa cggaagtgat
      721 tgatacttta gttgatgaaa ataaaggttg tccacgggat aaagtgcaaa cgcatgaaac
      781 acttaagcgt tatttacttg aagaaacatt cgaattgttc gaagctattg acaatgaaga
      841 tgattggcat atgattgaag aactaggaga tattttatta caagtgttat tgcatactag
      901 tattggtaaa aaagaagggt atatcgacat taaagaagtg attacaagtc ttaatgctaa
      961 aatgattcgt agacacccac acatatttgg tgatgctaat gctgaaacta tcgatgactt
     1021 aaaagaaatt tggtctaagg cgaaagatgc tgaaggtaaa cagccaagag ttaaatttga
     1081 aaaagtattt gcagagcatt ttttaaattt atatgagaag acgaaggata agtcatttga
     1141 tgaggccgcg ttaaagcagt ggctagaaaa aggggagagt aatacatgag attagataaa
     1201 tatttaaaag tatcacggtt aataaagcga cgtacgctag caaaagaggt aagtgatcaa
     1261 ggtagaatta caataaatgg taatgttgct aaagctggat cggatgttaa agttgaagat
     1321 gtgctgacga ttcgctttgg tcaaaaatta gtaacagtta aagtaactgc attaaatgaa
     1381 catgcatcta aagataacgc gaagggcatg tatgaaatca ttgaagagcg tcgacttgaa
     1441 gaagcgtaaa ttggaggtga caagcaatga aaaataaagt agaacatata gaaaatcagt
     1501 acacgtcgca agagaacaag aaaaaacaac gtcaaaaaat gaaaatgcgt gttgttcgta
     1561 ggcgtattac agtatttgcg ggtgtattac ttgcgataat tgttgtttta tcaatcttgc
     1621 ttgttgtcca aaaacatcgc aatgatattg atgcacagga gcgaaaagcg aaagaagcac
     1681 agtttcaaaa gcaacaaaat gaagaaattg cgttaaaaga aaagttgaat aatctgaatg
     1741 acaaagatta tattgaaaaa attgcgcgtg atgattatta cttaagcaac aaaggtgaag
     1801 tgatttttag gttgccagaa gacaaagatt cgtctagctc aaaatcttcg aaaaaataaa
     1861 tccaaattga ttcaaaatta tccgagtata gacattgtga aaaaatccaa acaaggatat
     1921 aataagggaa aatcgaatca aatcgggagg atttatttaa catatgtcaa tcgaagttgg
     1981 aaataagctt aaaggtaaag tcactggtat taaaaagttt ggtgcattcg tagaattacc
     2041 tgaaggaaaa agtggtttag ttcacattag tgaagtcgca gataattatg ttgaaaacgt
     2101 agaagagcac ctttctgttg gtgatgaagt agacgtaaaa gtattatcta ttgctgatga
     2161 tggaaaaatt agtctttcaa taaagaaagc taaagaccgt ccacgtagac aacatacgag
     2221 taaaccaagt catcaaaaac cagtgcaaaa agccgaagat tttgaaaaga aattaagcaa
     2281 tttcttaaaa gatagtgaag ataaattaac ttcaatcaaa cgtcaaacag aatctagacg
     2341 cggtggcaaa ggttctagac gttaattaaa ataaataaag actgtttcga taaggaatat
     2401 atttagaatg atgcgtatcg aataatcgat tgcagcgtta gacaatctaa gactgtttct
     2461 taaataagga gcagtctctt ttatttgtaa tgatataact aagacttata ccatttttga
     2521 aaattgtaaa agtgaggtga tgttatgcag ttaaatagta atggttggca tgttgatgac
     2581 catattgttg tcgctgtttc tacaggtatt gatagtatgt gtttattgta tcaactacta
     2641 aatgattata aagatagtta tagaaaacta acatgtttac atgtcaatca tggcgttagg
     2701 tcagcttcaa tcgaggaatc cagattttta gaagcatact gcgaacgtca tcacatcgat
     2761 ttacatatca aaaagttgga tttgtcgcat agtctcaatc gaaataacag cattcagaat
     2821 gaagctcgaa ttaaacgtta cgaatggttt gatgaaatga tgaatgtatt agaagcggat
     2881 gtattgctaa cggcgcatca tttggacgat caattagaaa ctattatgta tcgcattttt
     2941 aatgggaaat caacgcgtaa taaactagga tttgatgagt tgtcgaagcg aaatggttat
     3001 cagatttatc gaccactttt agctgtctct aaaaaagaaa taaaacaatt ccaagagaga
     3061 tatcatatac catattttga agatgaatct aataaagata acaaatatgt tagaaatgat
     3121 attcgtaata gaattattcc agctattgat gaaaataatc aacttaaagt atcgcattta
     3181 ttaaaattaa aacaatggca tgatgaacaa tatgatattt tgcaatattc agctaaacaa
     3241 tttattcaag aatttgtgaa gtttgatgaa cagtcaaaat atttagaggt ttctaggcaa
     3301 gcttttaata acttgccaaa ctcattaaag atggttgtgt tggattgcct attatcaaag
     3361 tattatgagt tgtttaatat tagtgctaaa acatacgaag agtggtttaa acaatttagt
     3421 agtaagaaag cacaattcag tattaatctc acggataaat ggataattca aatcgcatat
     3481 ggtaaattaa taataatggc taaaaataat ggcgatacat attttagagt tcaaactatt
     3541 gaaaagccag gtaattatat ttttaacaaa tatcgattag agatacattc taatttacca
     3601 aaatgtttat ttccacttac agtgagaaca cgacaaagtg gcgatacatt taaactgaat
     3661 gggcgcgatg gttataagaa agtaaatcgc ctgtttatag attgtaaagt gcaacagtgg
     3721 gttcgggatc aaatgccaat cgtattggat aaacaacagc gcattattgc ggtaggagat
     3781 ttatatcaac aacaaacaat aaaacaatgg attataatta gtaaaaatgg agatgaatag
     3841 cgttatgcat aatgatttga aagaagtatt gttaactgaa gaagatattc aaaatatctg
     3901 taaggaattg ggagcacaat taacaaagga ttatcaaggt aaaccattag tatgcgtggg
     3961 tatcttaaaa ggctcagcaa tgtttatgtc agatttaatt aaacgaattg atacccattt
     4021 atcaattgat ttcatggatg tttctagtta tcacggaggc actgagtcaa ctggtgaagt
     4081 tcaaatcatt aaagatttag gttcttctat tgaaaataaa gacgtattaa ttattgaaga
     4141 tatcttagag actggtacta cacttaagtc aattactgaa ttattacaat ctagaaaagt
     4201 taattcatta gaaatagtta ctttattaga taaaccaaac cgtcgtaaag cggacattga
     4261 agctaagtat gtaggtaaaa aaataccaga tgaatttgtt gttggttacg gtttagatta
     4321 tcgtgaatta taccgaaact taccatatat cggtacgtta aaacctgaag tgtattcaaa
     4381 ttaatttttt aatcgatttc agttattatt actatgcgtt tgagaaataa tagtgtagac
     4441 tcaaaaatat gaaaaatgta tttcatatat atttaatttt agacaagaca tatgtcttaa
     4501 aaagttgaaa aatatagaga ttgataaaac taacacaggg tgtgaatgac attgatgtta
     4561 agctcaatta ctagcttata aaacatgtca tatgttacaa tttttgttag ttttattatg
     4621 ggaagtagga ggaaatgacg catgcagaaa gcttttcgca atgtgctagt tatcgtaata
     4681 ataggcgtta ttatttttgg tctattttca tatttaaacg gtaatggaaa tatgccgaaa
     4741 cagcttacat ataatcaatt tactgagaag ttggaaaaag gtgaccttaa aactttagaa
     4801 atccaaccac aacaaaatgt ctatatggta agtggtaaaa cgaaaaatga tgaagactat
     4861 tcatcaacta ttttatataa caacgaaaaa gaattacaaa aaattactga tgctgctaaa
     4921 aagcaaaacg gtgtaaaatt aacgattaaa gaagaagaaa aacaaagtgt ctttgtgagt
     4981 atactttcaa cattaattcc agttgtagtc atagcgttat tatttatttt cttcctaagc
     5041 caagcacaag gtggcggtag tggcggtcgt atgatgaact ttggtaaatc taaagcaaaa
     5101 atgtacgata ataataaacg tcgtgttcgt ttctctgatg tagcaggggc agatgaagaa
     5161 aaacaagaat taattgaaat tgttgatttc ttgaaagaca ataaaaaatt caaagaaatg
     5221 ggatctagga ttcctaaagg tgtcttactt gttggacctc caggtactgg taaaacatta
     5281 cttgctagag cggttgcagg tgaagcaggc gcaccattct tctctattag tggttcagac
     5341 tttgtagaga tgtttgttgg tgttggtgcg agccgtgttc gtgacttatt cgataatgct
     5401 aagaaaaacg cgccttgtat catctttatc gatgagattg atgctgttgg tcgtcaacgt
     5461 ggtgcaggtg ttggtggcgg tcatgatgaa cgtgaacaaa ccctaaacca attattagtt
     5521 gaaatggatg gattcggtga aaatgaaggt atcattatga tagctgctac aaaccgtcct
     5581 gatatccttg acccagcctt attacgtcca ggtcgttttg atagacaaat tcaagttggt
     5641 cgtccagatg tgaaaggccg tgaagcaatt cttcatgttc atgctaaaaa taaaccactt
     5701 gatgaaacgg ttgatttaaa agcgatttca caacgtacac ctggtttctc aggtgctgat
     5761 ttagaaaact tattaaatga agcatcttta attgctgtac gtgaaggtaa aaagaaaatt
     5821 gacatgagag atatcgaaga ggcaacggat agagttatag ccggacctgc taagaaatct
     5881 cgagttattt ctaagaaaga acgtaatatt gttgctcatc acgaagctgg tcatacaatt
     5941 atcggtatgg tacttgatga ggcagaagta gtgcataaag ttactatcgt tccacgtgga
     6001 caagctggtg gttatgcaat gatgctacct aaacaagatc gtttcttaat gactgaacaa
     6061 gagttattag ataaaatctg tggtttactt ggtggacgtg tatcagaaga tattaacttt
     6121 aacgaagtat caacaggtgc ttcaaatgac ttcgaacgtg caacacaaat cgcacgctca
     6181 atggttacgc aatatggtat gagtaaaaaa ttagggccat tacagttcgg tcatagcaat
     6241 ggtcaagtat tcttaggtaa agatatgcaa ggtgagccta attattcaag ccaaatcgca
     6301 tatgaaattg ataaagaagt tcaacgaatc gttaaagaac aatacgaacg ttgtaaacaa
     6361 attttattag agcacaaaga acaattaatt ttaattgctg aaacattatt aacagaagaa
     6421 acattagttg ctgaacaaat tcaatcatta ttctacgaag gtaaattacc tgaaattgat
     6481 tatgatgcag ctaaagttgt taaagatgaa gattctgaat ttaatgatgg taaattcggt
     6541 aaatcttatg aagagattcg taaagagcaa ttagaagatg gacaacgtga cgaaagtgaa
     6601 gatcgtaaag aagaaaaaga tattgctgag gataaaaaag aagctgataa atctgatgaa
     6661 aaagatgaac cagcacatcg acaagcccca aatatcgaaa aaccttacga tccaaatcac
     6721 ccagacaata aataatcgat tatattcagt acctctttct atgataaagt tatagaaaga
     6781 ggtactttta tgatttaagg aatacgagcc tgagacataa a
//
LOCUS       DAMZPP010000143         6778 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMZPP010000143 DAMZPP010000000
VERSION     DAMZPP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.143"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..154
                     /locus_tag="POH73_002348"
     CDS             <1..154
                     /locus_tag="POH73_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HDI6888267.1"
                     /translation="KIDDLMNTYCNQCPIKTRLRKLEGKTKAHHFCINECSIGKEIKQ
                     LGNELQ"
     gene            166..417
                     /locus_tag="POH73_002349"
     CDS             166..417
                     /locus_tag="POH73_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HDI6888268.1"
                     /translation="MQIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            455..1579
                     /locus_tag="POH73_002350"
     CDS             455..1579
                     /locus_tag="POH73_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HDI6888269.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEENQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEDILPDIKAAFSGESQVLESGKDPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTKHDNIVFMRKWLDLGN
                     RYGNIEEVMDGVLEEVLATYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPAVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMTNRTI"
     gene            1595..2032
                     /locus_tag="POH73_002351"
     CDS             1595..2032
                     /locus_tag="POH73_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDI6888270.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEEVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQVP
                     SSPSYALFKGRDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            2456..3412
                     /locus_tag="POH73_002352"
     CDS             2456..3412
                     /locus_tag="POH73_002352"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDI6888271.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            3612..4091
                     /locus_tag="POH73_002353"
     CDS             3612..4091
                     /locus_tag="POH73_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDI6888272.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            4106..4945
                     /gene="fakB2"
                     /locus_tag="POH73_002354"
     CDS             4106..4945
                     /gene="fakB2"
                     /locus_tag="POH73_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HDI6888273.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTFNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSVGVAHANVIEYVDKLKKVFNEAFHVDNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            5031..5564
                     /gene="msrA"
                     /locus_tag="POH73_002355"
     CDS             5031..5564
                     /gene="msrA"
                     /locus_tag="POH73_002355"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDI6888274.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHADNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DKHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            5557..5985
                     /gene="msrB"
                     /locus_tag="POH73_002356"
     CDS             5557..5985
                     /gene="msrB"
                     /locus_tag="POH73_002356"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDI6888275.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMVRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            5997..6497
                     /locus_tag="POH73_002357"
     CDS             5997..6497
                     /locus_tag="POH73_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI6888276.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEYVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            6497..6718
                     /locus_tag="POH73_002358"
     CDS             6497..6718
                     /locus_tag="POH73_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDI6888277.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
BASE COUNT         2472 a          982 c         1252 g         2072 t
ORIGIN      
        1 gaaaatagat gatttaatga atacttattg caatcaatgt ccaatcaaaa ctcgtctgcg
       61 taaattagag gggaaaacga aggcgcatca tttttgtatc aatgagtgtt caatagggaa
      121 agaaataaaa caattaggaa atgaacttca ataggaggaa gtcaaatgca aattatatct
      181 atatcagaaa caccgaacca caacacaatg aagattacac ttagtgaaag cagagaaggt
      241 atgacatcag atacgtatac taaagttgat gattcacagc cagcatttat taatgacatc
      301 ttaaaggttg aaggcgttaa atcaattttc catgttatgg actttatttc agtagataaa
      361 gaaaatgacg caaattggga aacagtattg ccaaaagtag aggctgtatt cgaataaatt
      421 tttcatcaac tagtattcgg ggggaataaa gtatatggaa attttacgta tagagccaac
      481 acctagtcca aatacaatga aagttgtttt gtcatataca agagaagaca agttatctaa
      541 tacttataaa aaagtagaag aaaatcaacc aagatttata aatcagttgt tatctataga
      601 tggtatcact tccatttttc atgtcatgaa cttcttagct gttgataagg caccaaaagc
      661 tgattgggaa gacatactac ctgatattaa agctgctttt tcaggtgaaa gtcaggtgtt
      721 ggaatctgga aaagacccac aaattgacaa tcattttggt gaaattaaag ctgaactatt
      781 aacttttaag ggtataccgt atcaaattaa gctaacttct gctgaccaag aattaagaga
      841 acaattacca caaacatatg ttgatcatat gactcaagcg caaacaaaac atgacaatat
      901 tgtttttatg cgtaaatggc tagatttagg aaatcgctat ggaaatattg aagaagtaat
      961 ggatggtgtc ctagaagaag tactagctac ctatccagaa tcacagttac ctgtattggt
     1021 aaaacatgct ttagaagaaa atcacgcaac taataattat catttctatc gacatgtctc
     1081 tttggatgaa tatcatgcga ctgataattg gaagactcga ttaagaatgt taaatcattt
     1141 tccaaagccg acttttgaag atataccgct gcttgattta gctttatctg atgaaaaagt
     1201 accggttaga cgtcaagcga ttgtactatt aggtatgatt gaaagtaaag aaattttacc
     1261 gtatttatat aaggggcttc gtgataaaag tcctgctgta agaagaactg caggggattg
     1321 cataagcgat ttagggtatc cagaggcact accagaaatg gtgctactat tagatgatcc
     1381 acagaaaatc gttaggtggc gtgctgctat gtttatcttt gatgaaggta atgcagagca
     1441 gcttcccgca cttaaagccc atattaatga caatgcgttt gaagttaaat tacaaattga
     1501 aatggccata tctcgtattg aaaacggaga tgaagcactt ggttcagtat ggaaacaaat
     1561 gacgaacaga acaatttaat tggaggaatt aaatatgaat gcatatgatg cttatatgaa
     1621 agaaattgcg caacaaatgc gtggcgaatt aactcaaaat ggttttacaa gtttagaaac
     1681 gagcgaggaa gtatcagagt atatgaacca agtaaatgct gatgacacta cttttgtagt
     1741 tattaactct acatgcggct gtgcagctgg attagcaaga ccagctgcag tagctgttgc
     1801 aacacaaaat gaacatagac ctacaaatac agttacagtt tttgctgggc aagataaaga
     1861 agcaactgct acaatgcgag aattcattca gcaggtacca tctagtcctt cgtatgcttt
     1921 attcaaaggt cgagatttag tttattttat gcctagagaa tttatcgaag gtagagatat
     1981 taatgacatt gcaatggact taaaggatgc ctttgacgaa aattgtaaat agtacacata
     2041 aataaatata aaggttaaca cattttataa tattaaaaat ggtgtctgtc attgaaaata
     2101 gagaatatag ttgtattcca tttgttatat aaagaacgtt cttaccaact atattttcta
     2161 gaaatttaac tgttttaata ggacatcaaa cataatattc aaatcatgtg ttaacctctt
     2221 ttttaaaatt ttttagcatt aaagttatag atttgggtaa acaattagca attggaaaca
     2281 tagatcacgt tatgatgtgg caggtactta atcatcattt tataaataaa gtaacggaat
     2341 tcatgatatt aatatcatat tcctaaaatg agtgataaca aaatgctaca taaagttaaa
     2401 ttatatcgaa ctaaatatac atactataaa taataaaaat gaggtgttat cgcatatgtt
     2461 gaattcattt gatgcagcat atcacagtct ttgtgaagaa gttttagaaa taggaaatac
     2521 acgaaatgat cgcacaaata caggtacgat ttcgaaattt ggtcatcaac ttcgctttga
     2581 cttatctaaa ggatttccac tattaacgac aaagaaagtt tcttttaaat tagtagcgac
     2641 cgaattatta tggttcatta aaggagatac aaatatccaa tacttattaa agtataataa
     2701 taatatttgg aacgaatggg cttttgaaaa ttatatcaaa tcagacgagt ataaaggtcc
     2761 agatatgaca gatttcgggc atcgtgcatt gagtgatcct gaatttaacg aacaatataa
     2821 agaacaaatg aaacaattta agcaacgtat tcttgaagat gatacatttg cgaagcaatt
     2881 cggtgattta ggaaatgttt atggtaaaca gtggcgagat tgggttgata aagatggtaa
     2941 tcattttgat caacttaaaa cagtaattga gcaaattaag cataatccag attcaagacg
     3001 acacatcgta tctgcatgga atccaacaga aattgataca atggcacttc cgccttgtca
     3061 tacaatgttc cagttttatg ttcaagatgg taagttaagt tgccagttat accaacgtag
     3121 cgcagatatc tttttaggtg tgccatttaa tatcgcaagc tacgctttat tgacgcacct
     3181 tattgccaaa gaatgtggac ttgaagtagg agaatttgtg catacatttg gagatgcaca
     3241 tatttattca aatcatattg atgcgattca aacacaatta gcacgtgaaa gcttcaatcc
     3301 tccaacatta aaaattaaca gtgataagtc tatttttgac ataaattatg aagatttgga
     3361 aattgttgac tatgaatcac atccagcaat aaaagctcca atagcagtgt agtcgttgca
     3421 tagttagcta accatataga catcaaaatg acatcaaagt attttcaagt gcaaaaaagt
     3481 acttttttgt gttaaacgtt ttcataaatt atgcaaaatc attatttcta tcacacttta
     3541 tgataaaaat tgtgttaaat taaagataac ttagtaatta aaaatgaaat gatagaagaa
     3601 ggaggataat catgacttta tccattctag ttgcacatga cttgcaacga gtaattggtt
     3661 ttgaaaatca attaccttgg cacctaccaa atgatttgaa gcatgttaaa aaattatcaa
     3721 caggtcatac tttagtaatg ggtcgtaaga catttgaatc gattggtaaa ccactaccga
     3781 atcgtcgaaa tgttgtactt acttcagata caagtttcaa cgtagagggc gttgatgtaa
     3841 ttcattctat tgaagatatt taccaactac cgggccatgt ttttatattt ggagggcaaa
     3901 cattatttga agaaatgatt gataaagtgg acgacatgta tattactgtt attgaaggta
     3961 aatttcgtgg tgatacgttc tttccacctt atacatttga agactgggaa gttgcctctt
     4021 cagttgaagg taaactagat gagaaaaata caattccaca tacctttcta catttaattc
     4081 gtaaaaaata agggggaaaa cgaccatgac aaaacagatt atagtaacag actcaacatc
     4141 cgatttatct aaagaatatt tagaagcaaa caacatacat gtaattcctt taagtttaac
     4201 tattgaagga gcttcatacg ttgaccaagt agatattaca tcagaagaat ttattaatca
     4261 tattgaaaat gatgaagatg taaagacaag tcagccagcc ataggtgaat ttatatctgc
     4321 ttatgaagaa ctaggaaaag atggttctga aatcataagt attcatcttt cttcaggact
     4381 aagtggtaca tttaacactg cttaccaagc aagtcaaatg gtagatgcta atgtaactgt
     4441 tattgattca aaatctattt cttttggttt agggtatcaa atacaacacc tagtagagct
     4501 tgtaaaagaa ggtgtctcaa cttctgaaat agttaaaaag ttaaatcatt taagagaaaa
     4561 cattaaatta tttgtagtta tagggcaatt gaatcaatta attaaaggtg gcagaattag
     4621 taaaacaaaa ggtttgattg gtaatcttat gaaaattaaa ccaattggta cactagatga
     4681 tggtcgctta gagcttgtgc acaatgcgag aactcaaaat tctagtatcc aatacttgaa
     4741 aaaggaaatt gctgaattta taggagatca tgaaatcaaa tccgttggtg tcgcacatgc
     4801 taacgtcatt gaatatgttg ataaattgaa gaaagttttt aatgaagctt ttcatgtgga
     4861 taattacgat ataaatgtaa ctacaccagt tatttctgca catactggtc aaggtgcgat
     4921 tggcctcgta gtccttaaga agtaaattta atcttttcag tgttaattac ttccatttca
     4981 atcctttata gactaaattt ataattagat agatagagga ggtaattcat atgacaaaag
     5041 aatatgcaac attagcagga ggatgtttct ggtgcatggt taaaccattt acatcatatc
     5101 caggcatcaa gtcagtcgta tctggttata gtggcggtca tgctgataac ccaacttatg
     5161 aacaggtatg tacgaatcaa accggccatg tcgaagcagt acaaattacg tttgatccag
     5221 aggttacttc ctttgaaaat atattagaca tatatttcaa aacatttgac ccaaccgatg
     5281 atcaagggca atttttcgat agaggcgaaa gctatcaacc agtcattttc tatcatgata
     5341 agcatcagaa aaaggctgct gagtttaaaa agcaacaatt aaatgaacaa ggtattttca
     5401 agaaaccagt gattacacct attaaaccat ataaaaattt ctatccagct gaagactacc
     5461 atcaagatta ttataaaaag aacccggtac attattacca atatcaacgt ggttcaggta
     5521 gaaaagcgtt tatagaatca cattggggga atcaaaatgc ttaaaaaaga taaaagtgaa
     5581 ctaacagata tagaatatat tgttacacaa gaaaatggca ctgaaccacc atttatgaat
     5641 gaatattgga atcattttgc taaagggatt tatgtagata agatttctgg taaaccttta
     5701 tttacatctg aagaaaagtt tcattctgag tgcggatggc ctagcttttc caaagcgctt
     5761 gatgacgatg aaattataga attagtcgac aaatcatttg gtatggtaag aactgaagtg
     5821 cgttcagaag aatcaaatag tcatttagga cacgtcttta atgatggacc taaagaaagt
     5881 ggcggcttaa gatactgtat caattccgct gcaattcaat ttattccata tgaaaaatta
     5941 gaagaattgg gttatggcga tttaatatca cattttgata agtaggagtg gaaagtatgt
     6001 ttaaaaaatt attcggaaaa ggcaaagaag ttcagaaaga tatcgcaatt tatgcaccat
     6061 taactggaga atacgtgaaa attgaagata ttccagatcc tgtattcgca caaaaaatga
     6121 tgggcgaagg gtttggtatt aatccaactg aaggagaagt tgtgtctcca attgcaggac
     6181 gtgttgacaa tgtctttcca actaagcatg caattggttt aaaagcagat aacggcttag
     6241 aattattagt tcatatcggt ttagacacag ttcaattaga cggtgaagga tttgaagtgt
     6301 tagtgtctag tggtgatgaa gttaacgtcg gagatccatt agtaagattc aaccttgaat
     6361 atattaataa taatgctaaa tctgttattt caccaattat aattactaac actgatcaag
     6421 cggcttctat taatatttat gatgaaaatg ctgtgattaa aggtgaaact aaagtgattg
     6481 atgtgacaat gaactaatga aaaactattc gttttatcaa tttgtcatga cagtgcgtgg
     6541 tcgacacgac gataaaggtc gtctagcaga agagatattt gacgatcttg ctttcccaaa
     6601 acacgatgat gattttaaca tactgtctga ttatattgag acacatggtg atttcacatt
     6661 gccaatgtct gtatttgatg atctatatga agaatatacg gaatggctaa aattttaata
     6721 taatttttaa taataatagt tagaaccagg gtgatgcaag ttgttatcct ggttttaa
//
LOCUS       DAMZPP010000144         6652 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMZPP010000144 DAMZPP010000000
VERSION     DAMZPP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.144"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            504..839
                     /locus_tag="POH73_002359"
     CDS             504..839
                     /locus_tag="POH73_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6888278.1"
                     /translation="MITSLPMMNEVIGNPLLDKFMKDLIIQILAMISEQERNESKRRQ
                     AQGVQVAKEKGIYKGRPILYSPNAKDPQKRLVYYRVVELLEQGKSISTIAKEVGITRQ
                     TIYRIKNSK"
     gene            900..1214
                     /gene="arsR"
                     /locus_tag="POH73_002360"
     CDS             900..1214
                     /gene="arsR"
                     /locus_tag="POH73_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDI6888279.1"
                     /translation="MSYKELSTILKILSDSSRLEILDLLSCGELCACDLLEHFQFSQP
                     TLSHHMKSLVDNELVTTRKDGNKHWYQLNHAILDDIIQNLNIINTSNQRCVCKNVESG
                     DC"
     gene            1211..2503
                     /gene="arsB"
                     /locus_tag="POH73_002361"
     CDS             1211..2503
                     /gene="arsB"
                     /locus_tag="POH73_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HDI6888280.1"
                     /translation="MMTILAIVIFLLTLTFVIWQPKGLDIGITALIGAVVAIITGVVS
                     LSDVLEVTGIVWNATLTFVAVILISLILDEIGFFEWSAIHMVKASNGNGLKMFVFIML
                     LGAIVAAFFANDGAALILTPIVLAMVRNLGFNQKVISPFIIASGFIADTTSLPLIVSN
                     LVNIVSADYFDIGFIEYFSRMIIPNIFSLIASILVLWLYFRKSIPKTFDTENLSDPKS
                     AIKDSKLFKLSWIVLAVLLVGYLVSEFIQIPVSIIAGIIAFIFVILARKSKAVHTKQV
                     IKGAPWNIVVFSIGMYLVVFGLKNVGITTVLGDVLTNISNYGLFSSIMGIGFIAAFLS
                     SIMNNMPTVLIDAIAIGQSSATGILKEGMVYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKGVKISWGTYFKTGIIITIPVLFVTLLGLYLTLIIF"
     gene            2521..2916
                     /locus_tag="POH73_002362"
     CDS             2521..2916
                     /locus_tag="POH73_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent arsenate reductase"
                     /protein_id="HDI6888281.1"
                     /translation="MDKKTIYFICTGNSCRSQMAEGWGKEILGEGWNVYSAGIETHGV
                     NPKAIEAMKEVDIDISNHTSDLIDNDILKQSDLVVTLCSDADNNCPILPPNVKKEHWG
                     FDDPAGKEWSEFQRVRDEIKLAIEKFKLR"
     gene            3296..3589
                     /locus_tag="POH73_002363"
     CDS             3296..3589
                     /locus_tag="POH73_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDI6888282.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGK
                     HYVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
     gene            3664..5598
                     /locus_tag="POH73_002364"
     CDS             3664..5598
                     /locus_tag="POH73_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDI6888283.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNW
                     DKSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLT
                     PSDLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLV
                     PFIFLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTTIFFI
                     ANILIIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLTLKGKKG
                     YKFSIFMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNI
                     RNSEEKQILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNF
                     YKNINHDFKMIVNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSK
                     NVSVFSFDRTTNLKFGYLDSPIVMLLTPDDLTGDFYLAAVSQGGYLFKDLDTLKQLLK
                     DNHLEEEISGITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWN
                     KKQLLLRKIHGYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHL
                     LQIAYIKYLEKYFKDLMREI"
     gene            5602..5913
                     /locus_tag="POH73_002365"
     CDS             5602..5913
                     /locus_tag="POH73_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDI6888284.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVP
                     KDTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFL
                     K"
     gene            5925..6551
                     /locus_tag="POH73_002366"
     CDS             5925..6551
                     /locus_tag="POH73_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6888285.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLL
                     NIIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLGLALKFK
                     KNKKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTG
                     SLDTKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
BASE COUNT         2401 a          870 c         1060 g         2321 t
ORIGIN      
        1 ctatttttag taatatttat agccctaaca gttagataaa ctgcatttat aaaacccaaa
       61 atagtatatg gaattaaaat atataaattt tctaattgaa ctaagaaagt taacccaact
      121 aaaaataaaa cattcaaaac ggtagacata ataaaagcta tatctaactc atttttttat
      181 tcaaaataca ttcctccttt tttaaaattt taccatagtt gctttattga tgttgagcct
      241 cggaaccctt aacaatccca aaacttgtcg aatggtcggc ttaatagctc acgctatgcc
      301 gacattcgtc tgcaagttta gttaagggtt cttctcaacg cacaataaat tttctcggca
      361 taaatgcgtg tctatgcgta gacatggaga cacttcagaa ttatggagtg attacatgag
      421 tttaactatt tttctttatt cagttttagg aagtttatta gcaaattata tctacgaaaa
      481 atcttcgaaa agaagttcag ctcatgataa cgagtttacc tatgatgaat gaagtcattg
      541 gaaatccact actagataaa tttatgaaag atttaattat tcaaatttta gcaatgattt
      601 cagaacaaga aagaaatgaa agtaaacgtc gacaagctca aggggttcaa gtcgccaaag
      661 aaaaaggtat atataaaggg agacctattc tgtattctcc caatgctaaa gacccacaaa
      721 agcgtttagt ttattaccga gttgttgaat tacttgaaca gggtaaatct ataagtacta
      781 tagctaaaga agttggtatt acacgtcaaa ctatatatag aataaaaaat agtaaataaa
      841 ataacataga cgataatcta tatagatgtt aatctattta atgtctagga ggtttattca
      901 tgtcttataa agaactatca acaatattaa aaattttatc agattcaagt aggttagaaa
      961 tattagattt actttcttgt ggtgagctat gcgcttgtga cttattagaa cactttcaat
     1021 tctcacaacc tacactaagt catcatatga agtcattagt agataatgaa ttagttacaa
     1081 cacgaaaaga cggcaataaa cattggtatc aacttaatca tgctatttta gatgatatta
     1141 tccaaaactt gaacatcatt aatacatcta atcaaagatg tgtatgtaaa aatgtggaat
     1201 caggtgactg ttgatgacta ttttagcaat tgtaattttt cttttaactt taacctttgt
     1261 gatttggcaa ccaaaaggtt tagatattgg tattacagct ttaattggag ctgttgttgc
     1321 tatcattaca ggagtcgtaa gtctttctga tgtattagaa gtaacaggta ttgtttggaa
     1381 tgcaacttta acttttgtgg ctgttattct tatttcatta atattagatg aaattggttt
     1441 ttttgaatgg tctgcgatac atatggtcaa ggcttcaaac ggtaatggct taaaaatgtt
     1501 tgtttttatt atgttacttg gagcaattgt agcagcattt ttcgcaaatg atggtgcagc
     1561 tttaatctta acgcctattg tattagcgat ggtaaggaat ctaggattta atcaaaaagt
     1621 gatttccccc tttattattg ccagtggttt tattgctgat actacatcac ttcccttaat
     1681 tgtaagtaac ttagttaata tcgtttctgc agattacttc gatattggat ttattgaata
     1741 ttttagccga atgatcattc ctaatatatt ctctctgatt gctagtattc tcgttttatg
     1801 gttatatttc agaaaatcca tacctaaaac gttcgataca gaaaatctat cagatcctaa
     1861 aagtgcaatt aaagattcta agttatttaa gctttcatgg atagtattag cagtactact
     1921 cgttggatat cttgttagcg agtttatcca aattcctgta tcaattattg ctggtattat
     1981 tgcttttatc tttgtaatat tagctcgtaa atctaaagca gttcatacaa aacaagtgat
     2041 taaaggtgca ccatggaata tcgttgtatt ctctattggt atgtatcttg ttgtgtttgg
     2101 actaaaaaat gtaggtatta caacggtcct tggggatgtt ttaacaaaca tttcaaatta
     2161 tgggttattt agcagcatca tgggtatcgg ctttatagct gctttcttat cttcgattat
     2221 gaacaacatg ccaactgttt taatagatgc aatagcgatt ggccaatcca gtgctacagg
     2281 aatattaaaa gaaggcatgg tttatgcgaa tgtcataggt tctgatttag gacctaaaat
     2341 tacgccaatt ggttctttag caacattatt gtggctacat gtcttaacac aaaaaggtgt
     2401 gaagatttcg tggggaacat actttaaaac tggaattatc atcactattc cagtcctatt
     2461 tgtaacactc ttaggtttat accttacact aatcatattt taagaaaagg ggctttaatt
     2521 atggataaga aaacaattta ttttatatgt acaggaaact cttgtcgtag ccaaatggct
     2581 gaaggttggg gaaaggaaat attgggtgaa ggttggaatg tctattctgc tggtattgaa
     2641 acacatggtg ttaatcctaa agcaatagaa gctatgaaag aagtagatat tgatatatca
     2701 aaccatacgt cagacttgat tgataatgat attttaaaac aatcagattt ggtcgtaacg
     2761 ttatgtagtg atgcagacaa taattgtcct attttaccac caaacgttaa aaaagagcat
     2821 tggggttttg atgatccagc aggtaaagaa tggtcagaat tccaacgtgt tagagacgag
     2881 attaaattag ctatagaaaa gtttaaattg agataatcat aaacacctct tatttagtta
     2941 ataagaggtg ttttgccatt taaagaacaa aatttgtgcc atatacaata tgccctctct
     3001 ttttctcatt aacgaacgtt tataacgcaa tcatgatgag agtattaata cagttaatta
     3061 tttgaagtac tttagtttac ataatgttga attataggta gtgaaattgt atttattata
     3121 cattgtcata tcgttgttta atgatagtta atttaaggat aatagaatta atattatata
     3181 tagatgaaca aaacaagcaa cagagcaaaa aaacactttt agattatttt tactcattct
     3241 ctattttatt tatttttgct ataataaata aagaatatta aatggaggtg tttttatgaa
     3301 aaagaaattt gtttctagtt gtattgctag tacaatctta ttcggcactt tattaggtgt
     3361 aacttataaa gctgaagctg caacagtaca tgtagctggt ggtgtttgga gccatggtat
     3421 tggaaaacat tacgtatggt cttattatag tcacaataaa agaaatcatg gttcaacagc
     3481 agtagggaaa tattcatctt ttagtggtgt tgctcgacct ggtgttcaat ctaaggcatc
     3541 tgctccaaag gcttggggtg gaaacaaaac attttatagt cttcactaat tacatatatt
     3601 aattaaggag aatagacaga attaagtaaa cttaattctg tctattctct tgaaagaggc
     3661 aatatgaaaa aattcaaatt atttttagat tttagtactt tattattaat ttcagggcta
     3721 ttatttttat ttttcttcaa agaaaatgaa gaaattatac ctgaaagttc caatatttta
     3781 acaatctcta attgggataa atctaacagt aaatcaaaag tgttagatgt cattgaatca
     3841 ggcgccaaaa atcaaaatat acaaataatt aaatccgtta aagattttga taataaaaaa
     3901 gaattttttg ttttcaactc aaaacgaaat aattctgact ttatacgtaa taaaacatca
     3961 cttttaactc catcagattt attaaacaga gagataaaag gaaagtatta tattattggt
     4021 gaacatttca atgtagaaga gcttacgagt gaattaaaaa aagctggttt aacatctgaa
     4081 gttgaataca taaatagaaa catattattt tttgaattag tcatagataa tgatttatta
     4141 gtgcctttca tattcttagg cgtattatat cttttgtact tcttatatga cagaggatat
     4201 aatttgaaat tttatgctat tcaaaagcta catggctttt caactacacg cattattttt
     4261 cataatattc atataaaaat catttattgg ctagtactta ccactatttt ctttattgct
     4321 aatatattaa tcattcatat agccaattta gaattggatt tcttaatgtt tattcgtaga
     4381 tttatcgtgt tgctttttgt ttttgcttgt attactacat tgttgtggct gatttcgtat
     4441 agtcttcttt tgaagttaag tattccatta accttaaaag ggaaaaaagg atataagttt
     4501 tcaatattta tgggtacctt caccaaatgt atcatggtgc taatactatc ttttttactg
     4561 gcacaaaatt taaatgctta tacgaagtta aagaatatat atgactctga aaaaatctgg
     4621 caaaagttag ataattacta tacattagag atttctccta atattagaaa ttcagaagaa
     4681 aaacaaattt tagaaactaa tatatacaat ttaattaaga aaagcgaaca acttggtgga
     4741 ttgcttttaa agaataataa tatagccaat cccgataaaa acaattacat tcctgaaaat
     4801 ggcaatgtct tttttatcaa taacaatttt ctgaatttct ataaaaatat aaatcatgat
     4861 tttaaaatga ttgtcaatca ttcggataaa ataaacgtgt ttattcctcc aaggttacaa
     4921 tatcaaaaat ctgaaattca aaaaaatcac caaggatgga ttgactttca aaagcaacaa
     4981 aacaaaaaag tagatgtgca agtactgtca aaaaacgtat ctgttttttc ttttgatcgc
     5041 acaactaatt tgaaatttgg atatctagat tctccaatag taatgttgtt gacacctgac
     5101 gatttaacag gagattttta tttagctgct gtttctcaag gtggatatct atttaaagat
     5161 ttagatacac ttaaacagtt gcttaaagat aatcacctag aagaagaaat tagcggtata
     5221 actaattaca aggattctgt gttaaatgaa ttaaatgaaa cgaaaacaaa aatgataata
     5281 acaatagtta ctattattat taacgttttc attcttttga tagctactgt atttgaaaca
     5341 attcaatatt ttagctggaa taaaaagcaa ctattattaa gaaaaattca tggttattca
     5401 ttattcagca gtaatgtacg ttatttaaca atttctattt tactttctat tatgttagct
     5461 tacactacgc atattttatt tggttcaaag atattactat tcataataat gtctattgct
     5521 attattcaac atttacttca aattgcttat attaaatatc ttgaaaaata ctttaaagat
     5581 ttaatgaggg agatttaaac aatgaaaaga actttaatta ttgtaacaat tgtactatta
     5641 atcatcatac ttagtctgtt attggttaga aatgagcatt tagatcgttt taacccactg
     5701 ttaaaagaaa aagtatctta tgccaaagtt ccaaaagata cccaggacta tagaaatata
     5761 actgtatact ctaaaaatgg agaaaaaaag agttataaat tggatttttt aggttatgat
     5821 cctacaaaag aatatgtgaa agtaaatcat aaagggaaat atgtgcgttc gatagaatac
     5881 attacaaaag atattcctag ctttttaaaa tgaggtgacc gatagtgata aaacttgaaa
     5941 atgtttttgt aaaaaaaggt aataaaaaca ttttagacgg atgtaacttt aattttgaaa
     6001 aaggaaaatc atatgcttta gttggagaaa gtggcgctgg aaaatctact ttacttaaca
     6061 taatcgccgg gttcgaagat gtatcacaag gaagcatata tattgaagat aagttactaa
     6121 aaaagaaagt cgatttttat agatatacgt tagggtattt gtttcaaaat tttgctttac
     6181 ttgaaaatca aacaatcagt caaaatttag gtttagccct aaagttcaaa aagaataaaa
     6241 aagataatat gaacttaaag aaagaagtat taaaaaaggt cggcctagat ttagatatca
     6301 agagaattgt tagttctcta agcggtggtg aacaacaaag agtagcattg gcaagattaa
     6361 tattaaaaga tccgaaaatc attttagctg atgaacctac tggatcacta gatacgaaaa
     6421 atggaaaaat agttattgat ttattattga agctacttga tgaaaataaa actatgattg
     6481 tcgtaacgca tgatttagag cttgcgaaac gatttgatgt catagtcaat atttctgagc
     6541 ttagaagtta agaaatattg ataggtatag ccatacatag agaaaaaaca atatgaaata
     6601 tgattagtca ttacttttga gttttttaat ttagagtctg ggacataaat cc
//
LOCUS       DAMZPP010000145         6614 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMZPP010000145 DAMZPP010000000
VERSION     DAMZPP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.145"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(599..2086)
                     /gene="lysS"
                     /locus_tag="POH73_002367"
     CDS             complement(599..2086)
                     /gene="lysS"
                     /locus_tag="POH73_002367"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDI6888286.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            complement(2139..2231)
                     /locus_tag="POH73_002368"
     CDS             complement(2139..2231)
                     /locus_tag="POH73_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888287.1"
                     /translation="MVNGKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            complement(2625..3101)
                     /gene="folK"
                     /locus_tag="POH73_002369"
     CDS             complement(2625..3101)
                     /gene="folK"
                     /locus_tag="POH73_002369"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDI6888288.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISSIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRVRKERWGPRTLDVDILLYG
                     EEMIDLPNLSVPHPRMNERAFVLIPLNDIAANVIEPRSKLKVKDLVFVDDSVRRYK"
     gene            complement(3098..3463)
                     /gene="folB"
                     /locus_tag="POH73_002370"
     CDS             complement(3098..3463)
                     /gene="folB"
                     /locus_tag="POH73_002370"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDI6888289.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLSEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            complement(3441..4244)
                     /gene="folP"
                     /locus_tag="POH73_002371"
     CDS             complement(3441..4244)
                     /gene="folP"
                     /locus_tag="POH73_002371"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDI6888290.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAVTRVKAMMDEGADII
                     DVGGVSTRPGHEMITVEEELNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDII
                     NDQWAGLYDHRMFQVVAKYDAEIVLMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNFS"
     gene            complement(4460..5392)
                     /gene="cysK"
                     /locus_tag="POH73_002372"
     CDS             complement(4460..5392)
                     /gene="cysK"
                     /locus_tag="POH73_002372"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDI6888291.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            complement(5571..6452)
                     /gene="hslO"
                     /locus_tag="POH73_002373"
     CDS             complement(5571..6452)
                     /gene="hslO"
                     /locus_tag="POH73_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDI6888292.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
BASE COUNT         2034 a         1379 c          847 g         2354 t
ORIGIN      
        1 ggcatgggaa caggtgtgac ctccttgcta tagccaccag acaaaataac ttacttattt
       61 atgatacacg cttaattaat aaggcgcaat agttaatttt acactttgtt gtgaactttg
      121 tcacatcagt ttttagtgtg tttcgtctgt ttttgacgac ttttatatat tacttcattc
      181 aattgataaa gtcaacactt ttaatcaact tttttattct tcaaaactga catttctcgt
      241 gctctctaat tcataacact tttagtattc tatcatacac atatgtacaa aaccatacac
      301 attctataca attttaaagt acttttatag ctgattaaga taaattatac taatatagaa
      361 atttttgtta taagatatct caatgtctat cattaacatt tctcatcaga actaatctat
      421 atacgtatcg tcattttcat ctaacagcta actaccttgt ttaacagtca aaaatagcac
      481 cattatcgtt aactattact atcaaaaaca ttcaaacacg aactaattac cataattaag
      541 aaaaccctcg catacacatg ttgtatacac gagagctctt actaacaatc aacgtcattt
      601 atttttgtct catataaggg aataataata cgtctctgat tgatggagag tcagttaata
      661 acataactaa tctgtcaata ccgataccaa gaccacctgt cggaggcata ccatattcta
      721 acgcttcaat gtaatcttca tccatttcat gcgcttcatc attaccttgc gctttttcaa
      781 caagttgcgc ttcaaaacga cctttttgat caataggatc atttaattca gtaaatgcat
      841 ttgcatgctc tctacctaca atgaacaatt cgaaacgatc agtaaatcta ggatcttcag
      901 gatttttctt cgctaaaggt gaaatttcag tcggatgacc atagataaac gttggctgaa
      961 taagtgtttc ttcaactttt tgctcaaaga attcatttaa aatatgacca tatttcattg
     1021 tatctttaat ttcaatacca tgttccttag ctaaagcttt agcttcttca tcacttttaa
     1081 cttcataaaa atctacacca gtagcttctt ttacagcatc aacaatatgc aaacgagtcc
     1141 aagcagattc taaatcaatc gtttccccat tgtattgtac ttttgcagaa cctaatactt
     1201 cattggcaat atgtctcacc atagattctg ttaaatccat aatgtcatga taatctgcat
     1261 atgcttcata taattcaatc attgtgaatt cagggttatg tctagttgat acaccttcat
     1321 tacggaatac tctaccaatt tcatatactt tttcaagtcc accgacaatt aaacgtttta
     1381 aatgcaactc aatagcaata cgcatgtata acgttgcatc taatgcatta tgatgtgtta
     1441 caaatggtct agcagctgct ccaccagcaa tttggtgcat cataggtgtt tctacttcca
     1501 agaaaccttt attatttaaa taattacgca tttcttgaat gattttacta cgattaataa
     1561 atgtacgagt gctatcttcg ttcgtaatta aatctaaata tctttgacga tatctctgtt
     1621 caatatcctg taaaccgtgg aatttatccg gtaatggtcg caatgattta gttagtagcg
     1681 tgaatttctt cgctttaacc gataattcgc cagtatttgt tttgaacatt acaccttcaa
     1741 caccaacgat atcgcctaaa tcagcatttt tccataaatc aaattcgtca tcgccaactt
     1801 gatctttacg aacgtatatt tgaatttgtc cagctaagtc ctgaacgtgt gcaaatcctg
     1861 ctttaccttt accacgctta gtcattaatc gtccagctat agcgacatga ctatccgctt
     1921 ctttttctac caattcttct ttagaatact ggtcccactc ttctttcaaa tcactagata
     1981 aacctgaacg gtcaaattta gaaccaaacg ggtctatacc aagatcatat aattcttgta
     2041 atttttgacg tcgaaccaac atttggtcat tcatttcttc tgacataact ttctctcctt
     2101 taacttgttg taatccttta gtattccaac tggtacactc aaacattttc ctttaaagtt
     2161 ttataaaata tcagacattt ttgctcaaca tacatctctt tcgatgtttc taatacttta
     2221 ccattaacca ttgcacctta aagttatgat gtggcatgtt ttcttatatt cataacatca
     2281 attttatcac ttttttaatt ttacaagagg ttgtaaatgt aataaagcaa tttctgtttt
     2341 cgtttatgaa ttacttattc atttaaaata cgattatcat taatacaacc ctaagtaaat
     2401 gtataaaaat tatctttcac aaacttcaac aaaagtccaa ataaattaca gcaatttatc
     2461 aaatattgct tactttgatt ttatgaaata acttaattct aacacatact aaatcatcat
     2521 atactaattc gaaatcaaat gcatttagag ataatcaaaa tgccgaaaca tctccaataa
     2581 tcaatcatct attcctaata aatatgaatg ttctcaacaa tacattattt atatcgcctt
     2641 acactgtcat cgacaaaaac taaatctttc actttcaatt tcgaacgtgg ttctatgaca
     2701 tttgctgcta tatcatttaa tgggattaaa acaaatgcac gttcattcat tctcggatgt
     2761 ggcaccgaca gatttggtaa atctatcatt tcttctccat acaacaaaat atccacatct
     2821 aaagttctag gaccccatcg ttcctttcta acacggtgta aacattcttc tgtcttcaaa
     2881 caacattcca acagttgtaa tactgtgagt gttgtttgaa tctcaacaca caaatttaaa
     2941 aagttaggtt gctcagtata cccaactggt gctgtttcat aaatcgaaga aatattagat
     3001 acgttaatac catcatattc attcaaaatc tttatagcat cgtttaactg gctttctcta
     3061 tcaccaatat tactacctaa tcctaagtat gcttgaatca tttattctcc ctcacaattt
     3121 cgatacctac tccatcataa tgacccggaa tcggtgggtt ttctttagtg attctcactt
     3181 tcgtttccat tacacgatta tattgtgaat ttatacgatt tgcaatacgt tcagctagat
     3241 gctcaagtaa attaacggcc ttaccttcca taattgattt aacctcttcg aacacttcac
     3301 cataatgaac tgtatcaata acattatcag tacgaccagc ttcagataag tctactttca
     3361 aagttacatc cactttgaaa atttgtccta tttcattttc agctgataaa gcaccatgat
     3421 atccataaaa gcgcatacct ttaagaaaaa ttgtgtcttg cattttcatt ctcctttaaa
     3481 aaatctatac ctttagctaa tttagcattc aactcgacat tatgaacgcg tactgctcta
     3541 acgcctttca taataccata tgcagtcgta gctgcagtta cttcatctct ttcaaccggt
     3601 gttgtatcat aacccatcat ctctttagtg aaacgtttcc ggcttgtcgc taataaaact
     3661 ggatattctg ttgcaacaag ttcatccagt cttgccataa cttcggcttc ttcatttcta
     3721 gttttagcga aacctatacc tggatctagc caaattttat ttgaaggtat accagctatt
     3781 ttagcttgat gtgcttgtgc taacaaagat gttaacattt cttcgacaac cggttcatca
     3841 cgattaccat tgccattatg cattaaaaca atttccgcat cgtatttggc tacaacttga
     3901 aacatgcgat gatcataaag gcctgcccat tgatcattaa taatatcaac acctagcttt
     3961 aaacaagctt cagcaacttc actacgaaaa gtatcaaccg aaatttttac atcaaaaccg
     4021 acaatcgctt caacaactgg taatactcta ttcaactctt cttccacagt aatcatttca
     4081 tggccgggtc tcgttgaaac acctccaaca tctataatgt cagcaccttc atccatcatc
     4141 gctttcactc tagttactgc tgattcaaca ttattaaatt ttccaccatc tgagaatgaa
     4201 tcaggtgtga cgtttaatat gcccataatt tttgttttag tcatgttaac caccctttaa
     4261 ttatgtattg tttaactcta ataaaattta ctcctgcgat ttactagtta ttagtatgta
     4321 cgtttattat cttatcattc actggatgtt tttatcagaa aaatgtttaa tttaacgctc
     4381 atatttttaa aattatacat ggtagaaaaa aagagcaagt tatctcaaaa agatagctca
     4441 ctcttttaaa tgacattaat tagtcatcga aagaatataa aggtgttgat aagtagcgtt
     4501 caccattact cggcaatact gttactactg ttttaccttt tcctaattct tttgcttttt
     4561 gaatggcagc ataaatcgca gcacctgatg aaatacctgc taaaatacct tcctctttag
     4621 caactcgacg agacatttcc atcgctgtat catttcctac tttaataata ctgtcataga
     4681 tttctgtatt caaagtacct ggaataaatc cagcacctaa accttgtaat ttatgtggac
     4741 ctggctcacc accgctcaat actggagaag cctcaggctc tatagcaaca atttcgatgt
     4801 taggatattc ttttttcaga actttaccta caccagataa cgtaccgcca gtaccaacac
     4861 cagctaggaa cgcatcgata gttttccctt caaattgttg taataactca ggacctgtag
     4921 ttaactcatg aacttcaggg ttcgcagggt tttcaaattg ttgtggctcg aagtaaccat
     4981 gttcttcttt taattcttta gcttttttaa ttgcaccttt catcgcttct gatccaggcg
     5041 ttaagactaa ttccgcaccg tatgctttta ataaattacg acgctcttgg ctcattgttt
     5101 caggcatagt aaatactgct ttatatcctt tagcagcaca tacaaatgct aaaccgatac
     5161 ctgtattacc actagttggt tctacaattg tatcgccagg tttaattttg ccttctcgct
     5221 ctgctttttc aatcattgct aaagcaattc tatcctttac agaaccacct ggattttgat
     5281 attccaattt tacataaaca tctgctgcat tgtcatctac tacatttctc aatttgacta
     5341 ccggtgtacc gccaataatt tgagtaatat tatctactgg tttttgtgcc attaagaaca
     5401 ctccttttta gttagttgtt gaaatcttag tttatttatc ggatatagaa ttattttatc
     5461 aaataaattc tagaaagcaa acaaaattac taaggattat acaactattt agttctacat
     5521 ttcactaaac cgctaaaaat aaacatttta gcgtattgat ttaaattaaa ttacgctaaa
     5581 ctttctagca acacgtttaa ttcttcttca gtatatttat atttatttcc acagaaatga
     5641 catactgctt cagcaccatg atcttctttg atcatatttt gaatctcagc ctcgcccaat
     5701 cctttaatag catttaaaaa tttctcatga ctacaattac attcaaattg aacaggcatt
     5761 ttctctaaaa tttgcacatg gtcttcacct aagatttcgt ttagtaatcc ttctggcgtt
     5821 aatccttgtt caattaattt agaaactggt gtcatttcac taattgcttt ttctaatttt
     5881 gaaattgttt catctttagc acctggcata acttgaatga taaatcctcc tgctgcttta
     5941 atcgtattat caggatttac caatacacca agacctaccg atgaaggtgt ttgttcactt
     6001 gtagcataat aataagtaaa atcttcacca agttctcctg aaacaattgg acttgctcca
     6061 gagaaatagt ctttcattcc aacgtcttta acaaccataa tagatccatt tgtccctact
     6121 gctcgtctta catcaagttt accttgctca tttaatggaa aatgagtttg tggatggtct
     6181 acataagcgc gcacctcgcc tttagcattt gcatcggcaa taattcgtcc aataggtcct
     6241 tggccatcta cagtgactgt taatttttga tcacctttca acattgcgcc catcatagct
     6301 gttgctgtca ttgttcttcc cattgcagca gatgctgtcg gccatgtata atgtctcgtt
     6361 tgtgcttctt gaacagtttc agttgtcaaa gcagcataag ccctaatctc cccatcaaat
     6421 gctaatgctt taacaatata atcgtgtgtc attatttcaa tctcctctat tactctatat
     6481 tttaaaaatt actttacttc ataaaatgca acaattgtac ttattctaca cccatcattc
     6541 taaataatga agcaacttgt ttgattatta attattcgtg ccataatggg agtgggacag
     6601 aaatgatatt ttca
//
LOCUS       DAMZPP010000146         6500 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMZPP010000146 DAMZPP010000000
VERSION     DAMZPP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.146"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..654
                     /locus_tag="POH73_002374"
     CDS             <1..654
                     /locus_tag="POH73_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HDI6888293.1"
                     /translation="KERTSKERMLYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPF
                     KQTLHEAITMSGADANMTQILEEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKF
                     KIATPVFKPLFAWQMSGILLWIYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQG
                     LSVIHFFGKAKGLPNAVTILLLVIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            669..2636
                     /gene="gdpP"
                     /locus_tag="POH73_002375"
     CDS             669..2636
                     /gene="gdpP"
                     /locus_tag="POH73_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HDI6888294.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIVLFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            2633..3079
                     /gene="rplI"
                     /locus_tag="POH73_002376"
     CDS             2633..3079
                     /gene="rplI"
                     /locus_tag="POH73_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDI6888295.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            3111..4511
                     /gene="dnaB"
                     /locus_tag="POH73_002377"
     CDS             3111..4511
                     /gene="dnaB"
                     /locus_tag="POH73_002377"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDI6888296.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            4789..6072
                     /locus_tag="POH73_002378"
     CDS             4789..6072
                     /locus_tag="POH73_002378"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDI6888297.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTSVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
BASE COUNT         2264 a          979 c         1304 g         1953 t
ORIGIN      
        1 aaagaaagaa cgtctaaaga aagaatgtta tacgtaacaa ccgtagcgat gagcttaatt
       61 tcattaatcg cctttatgtt actacaaaca ttcggaagaa ttccaccatc agcgagcata
      121 gtaaaacctt tcaagcaaac attacatgaa gcgattacga tgagcggtgc cgatgcgaat
      181 atgacccaaa tattagaaga agggtttaga caagcgaccg ttcaattacc aggtttcatc
      241 attatcatta catttttaat cgtcttaatt aacttaatcg ttacatttcc gattttacga
      301 aaatttaaaa tcgctacacc tgtatttaag ccacttttcg cgtggcaaat gagcggtatt
      361 ttattatgga tatacattat tgttatcata tgtttattat ttacaggtca accgagtgtg
      421 ttccagagca ttcttttaaa tttccaactt gtgttatcat tagtgatgta tattcaaggt
      481 ttaagtgtta ttcatttctt tggtaaagcg aaaggtttgc cgaatgcagt aacgatttta
      541 ctattggtta tcggtacaat actgacacct acgacgcata ttgtaggact acttggtgtt
      601 atcgatttaa gtttgaattt gaagcgaatc atgaaaaata attctaaaaa gtgaatagag
      661 gtggaataat gaatcggcag tccactaaga aagctttact aataccattt gtcatcatga
      721 tcatcacagc aattgtttta atgggtgtat ggtttatctt taatagcctt atagcattaa
      781 ttgcatctat cgttcttgtc gtgatgatta ttgttagcat cgttttattc agacaagctt
      841 taatgaaaat ggatagttat gtagatggtt tgagtgctca aatttcaaca acaaataata
      901 aagcaatcaa acatttacca attggtatca ttgttttaga tgaaaatgat cacatcgaat
      961 gggttaacca atttatgaca gatcatatgg aagcaaatgt catttctgaa tctgtaaatg
     1021 aagtatttcc aaacatttta aagcaattag atagagtgaa atccgttgaa atagaatata
     1081 atcagtatca tttccaagta cgttattctg agaatgatca ctgcctctat ttctttgata
     1141 taactgaaca agtacaaaca aatgaactat atgaaaattc taaaccaatc attgcgacat
     1201 tatttttaga caactacgat gagattacgc aaaatatgaa tgatacgcag cgttcggaga
     1261 tcaattcaat ggtaacgcgt gtcattagtc gatgggcaac tgagtataat atattcttta
     1321 aaaggtatag ttccgatcaa ttcgtagcct atttaaatca aaaaatatta gctgacttag
     1381 aagaatctaa atttgatatc ttgagtcaat tacgtgaaaa aagtgttggt tatcgtgccc
     1441 aattaacatt aagtatcggt gttggtgaag gcactgaaaa tttaatcgac ttaggtgaat
     1501 tatcacaatc aggcctagac ttagcattag gacgtggtgg tgaccaagtt gcaattaaaa
     1561 gtattaatgg taatgtgcgc ttctatggcg gtaagactga cccgatggag aaacgtactc
     1621 gtgtaagagc acgtgttatc tcacatgcgt taaaagacat ccttgcagag ggtgacaaag
     1681 tcattatcat gggacataaa cgtcctgact tagatgcaat tggtgcagca atcggtgtgt
     1741 ctagatttgc aatgatgaat aatttagaag catacatcgt attaaatgag actgacattg
     1801 atccaacatt acgacgcgtg atgaacgaaa ttgataaaaa gccagagtta agagagcgat
     1861 ttattacatc agatgatgct tgggatatga tgacatctaa gacaaccgta gtgattgttg
     1921 atacgcataa accggaactg gttttagatg aaaatgtctt aaataaagca aaccgtaaag
     1981 ttgttatcga tcatcataga cgtggtgaaa gcttcatctc taatccattg ttgatatata
     2041 tggaaccata cgcaagttca acagcagaac ttgtaacaga attactggaa tatcaaccaa
     2101 cagaacaacg tttaacgcgt cttgaatcaa cagtgatgta tgcaggtatt attgtagata
     2161 caagaaactt tacattacga acaggatcaa gaacattcga tgcagcgagt tatttacgtg
     2221 cacatggtgc agatacgatt ttaacgcaac atttcttaaa agatgatgtg gatacttaca
     2281 ttaatcgatc tgaattaatt cgaactgtaa aagttgaaga taatggcata gccattgcgc
     2341 atggttcaga cgataaaatt tatcatccag taacagttgc acaagcagca gatgaactgt
     2401 taagtttaga aggtattgaa gcatcatatg ttgttgcgag acgtgaagat aatctgattg
     2461 gtatatctgc gcgttcactc ggttcagtaa atgtccagtt aacaatggaa gcacttggtg
     2521 gcggtggaca tttaaccaat gctgcaacac aacttaaagg tgtgacagtc gaagaggcga
     2581 tagcgcaatt acaacaagca attacagaac aattaagtag gagtgaagat gcatgaaagt
     2641 aatttttaca caagatgtta aaggtaaagg taaaaaaggt gaagttaaag aagtaccagt
     2701 aggttatgca aataacttct tattgaaaaa gaattatgct gtagaagcaa caccaggtaa
     2761 cctgaaacaa ttagagttac agaaaaaacg tgcaaaacaa gaacgccaac aagaaattga
     2821 agatgctaaa gcattaaaag aaactttatc aaacattgaa gttgaagtat cagcaaaaac
     2881 tggtgaaggt ggtaaattgt ttggatcagt aagcacaaaa caaattgccg aagcactaaa
     2941 agcacaacat gatattaaaa ttgataaacg taaaatggat ttaccaaatg gaattcattc
     3001 cctaggatat acgaatgtac ctgttaaatt agataaagaa gttgaaggta caattcgcgt
     3061 acacacagtt gaacaataaa gttggattga aataagaggt gtaaccattc atggatagaa
     3121 tgtatgagca aaatcaaatg ccacataaca atgaagctga acagtctgtc ttaggttcaa
     3181 ttattataga tccagaattg attaatacta ctcaggaagt gttgcttcct gagtcatttt
     3241 ataggggtgc ccatcaacat attttccgtg caatgatgca cttaaatgaa gataataaag
     3301 aaattgatgt tgtaacattg atggatcaat tatcgacgga aggtacgttg aatgaagcgg
     3361 gtggcccgca atatcttgca gagttatcta caaatgtacc aacgacgcga aatgttcagt
     3421 attatactga tatcgtttct aagcatgcat taaaacgtag attaattcaa actgcagata
     3481 gtattgccaa tgatggatat aatgatgaac ttgaactaga tgcgatttta agtgatgcag
     3541 aacgtcgaat tttagagcta tcatcttctc gtgaaagcga tggctttaaa gacattcgag
     3601 acgtcttagg acaagtatat gaaacagctg aagagcttga tcaaaatagt ggtcaaacac
     3661 caggtattcc tacaggatat cgagatttag accaaatgac agcagggttc aaccgaaatg
     3721 atttaattat ccttgcagcg cgtccatctg taggtaagac tgcgttcgca cttaatattg
     3781 cacaaaaagt tgcaacgcat gaagatatgt atacagttgg tattttctca ctagagatgg
     3841 gtgctgatca gttagccaca cgtatgattt gtagttctgg taatgttgac tcaaaccgct
     3901 taagaaccgg tactatgact gaggaagatt ggagtcgttt tactatagcg gtaggtaaat
     3961 tatcacgtac gaagattttt attgatgata caccgggtat tcgaattaat gatctacgtt
     4021 ctaaatgtcg tcgattaaag caagaacatg gcttagacat gattgtgatt gactacttac
     4081 agttgattca aggtagtggt tcacgtgcgt ctgataacag acaacaggaa gtttctgaaa
     4141 tctctcgtac attaaaagca ttagcccgtg aattagaatg tccagttatc gcattaagtc
     4201 agttatctcg tggtgttgaa caacgacaag ataaacgtcc aatgatgagt gatattcgtg
     4261 aatctggttc gattgagcaa gatgccgata tcgttgcatt cttataccgt gatgattact
     4321 ataaccgtgg tggcgatgaa gatgatgacg atgatggtgg tttcgagccg caaacgaatg
     4381 atgaaaacgg cgaaattgaa atcatcattg ctaagcaacg taatggtcca acaggcacag
     4441 ttaagttaca ctttatgaaa caatataata aatttacaga tatcgattat gcacatgctg
     4501 atatgatgta aaaaagtttt tccgtacaat aatcatttta gtgataaaat tgtacggttt
     4561 ttattttgtt ctgaacgggt tgatatatgt taagtttgtg tattggaagt gatgaattag
     4621 tgctgtcaaa gactctgtta aagggttttg aggacgttga agacgatttg ttaaaatgat
     4681 ttttctttta aaaaggctga aaatcaatgt tcgattttta tttgcattat ggtctcgata
     4741 ttggtagaat atcaaatggt taaatgagaa aaacttggag gtgctcacat gtcatcaatc
     4801 gtagtagttg ggacacaatg gggagacgaa ggaaaaggaa aaataacgga tttcttggca
     4861 gaacagtcag atgttatcgc gcgtttttca ggtggtaata atgcaggcca taccattcaa
     4921 tttggcggag aaacatataa attacattta gtaccatctg gtatctttta caaagacaaa
     4981 ttagcggtaa tcggtaacgg agtcgttgtt gatccagttg cactattgaa agaattagac
     5041 ggattaaatg aacgtggcat tcctacaagt aatttacgta tatctaatcg tgcgcaagtg
     5101 attttaccat atcacttagc acaagatgaa tatgaagaac gtttacgcgg tgataataag
     5161 attggtacaa ctaaaaaagg tatcggtcca gcatatgtag acaaagttca acgtatcggt
     5221 attcgtatgg cagatttact tgaaaaagaa acattcgaaa gattattaaa atcaaacatt
     5281 gaatataaac aagcatattt caaaggcatg tttaacgaaa catgcccatc atttgatgat
     5341 atctttgaag aatattatgc agcaggtcaa cgtctaaaag aatttgtaac agacacatca
     5401 aaaatcttag acgatgcatt tgtagcagat gaaaaggtac ttttcgaagg tgcgcaaggt
     5461 gtaatgttag atatcgacca tggtacatat ccattcgtta catcaagtaa tccaattgca
     5521 ggtaacgtta ctgttggtac aggtgtaggt cctacattcg tttcaaaggt aattggtgta
     5581 tgtaaagctt atacatcacg tgttggtgat ggtccattcc ctactgaatt attcgatgaa
     5641 gatggacatc atattagaga agttggtcgt gaatacggta caacaacagg acgtccacgt
     5701 cgtgtaggtt ggtttgattc agttgtatta cgtcactctc gtcgtgtaag tggtattaca
     5761 gatttatcta ttaactcaat tgatgtttta acaggcctag acacagtgaa aatctgtaca
     5821 gcttatgaat tagacggtaa agaaattact gagtacccag caaacttaga tcaattaaaa
     5881 cgttgtaaac caatctttga agagctacca ggttggacag aagacgtaac aagtgtgcgt
     5941 actttagaag aattacctga aaatgcacgt aaatatttag agcgtatttc agaattatgt
     6001 aatgtacaaa tttctatctt ctcagttggt ccagatagag aacaaacaaa tctattaaaa
     6061 gaattgtggt aaaactttat ataagtcata cacaatgatt ataaatacat gagccttcta
     6121 tctttattgg taggaggctt ttgttatgtt tgcttctgta tcgattcgat tatttagata
     6181 aaaaacacta acgtaaaggt ggtatttgct agtcataatt tagaagatta gatgatattt
     6241 aatgaaaatt aagataaaat acttgaatgt aggaagtctg atgtcgaaaa tgactattaa
     6301 aatagagtag acgtaagtgt gaatgcaagt atctaaaata gaaaaatttc gaaaatagcg
     6361 taattattat aataaataga ctgccaataa aatgcaattt ttcacttata acattcttca
     6421 aaaaataata gcaaaattat gtaaaaaata tcttgtcatg gcaagattgg ctgtgctata
     6481 atctatcttg tgcttaagaa
//
LOCUS       DAMZPP010000147         6336 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMZPP010000147 DAMZPP010000000
VERSION     DAMZPP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.147"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(343..450)
                     /locus_tag="POH73_002379"
     CDS             complement(343..450)
                     /locus_tag="POH73_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDI6888298.1"
                     /translation="MFNLLIDIMTSALSGCLVAFFAHWLRTRNNKKGDK"
     gene            complement(584..970)
                     /gene="gtxA"
                     /locus_tag="POH73_002380"
     CDS             complement(584..970)
                     /gene="gtxA"
                     /locus_tag="POH73_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HDI6888299.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLRIEYMISHI
                     TGFIVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNISLQTVLLYVFVSWLNLPAE
                     IAPFAGLIITIPITFILSKWILKDSN"
     gene            1238..2380
                     /locus_tag="POH73_002381"
     CDS             1238..2380
                     /locus_tag="POH73_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDI6888300.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGKTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGDNLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            2440..3099
                     /locus_tag="POH73_002382"
     CDS             2440..3099
                     /locus_tag="POH73_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888301.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            3281..4492
                     /locus_tag="POH73_002383"
     CDS             3281..4492
                     /locus_tag="POH73_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDI6888302.1"
                     /translation="MQSSKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILASGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTPKEELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(4615..5088)
                     /locus_tag="POH73_002384"
     CDS             complement(4615..5088)
                     /locus_tag="POH73_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDI6888303.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYTIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            5201..5857
                     /locus_tag="POH73_002385"
     CDS             5201..5857
                     /locus_tag="POH73_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDI6888304.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPDAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQKQNKHT
                     "
     gene            complement(5920..>6336)
                     /locus_tag="POH73_002386"
     CDS             complement(5920..>6336)
                     /locus_tag="POH73_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDI6888305.1"
                     /translation="LQRGKHYGATWQDKERISWGTSDQNLSKIAAEQVKKDLLEVGNR
                     RVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDIPYSIMGHVHFRKSV
                     MDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
BASE COUNT         2093 a         1178 c          995 g         2070 t
ORIGIN      
        1 tgttggggcc ccgccaactt gcattgtctg tagaaactgt ggatccaatt tctctatgtt
       61 gggtccctga gtatagcctt gtagagtcta gtacattgat ttgtatccca atgtccctat
      121 aattgattat tcgctttatc taatgatcct atgactcaac tattaaatca tttttcgaaa
      181 tacttaattc taatgtaatt aaatccattt attgtaatat tgcaaaaata cattgcacac
      241 cttgttcatc aatgctataa ttagttacat aataaattga acatctaaat acaccaaatc
      301 ccctcactac tgccatagtg aggggattta tttaggtgtt ggttatttgt cacctttttt
      361 attgttgcgc gttcgtaacc aatgtgcaaa aaacgcaaca agacagccgc ttaaagctga
      421 agtcatgatg tcaattaata aattgaacat ccgtcataca cctcctctct gcgttaaagt
      481 aacgcccgag atgttaggcg accattatat tatatcattt atttattata tttcacgcaa
      541 tattaagacc cttccaaagt attttttagt ggtttacgct actttaatta ctatctttta
      601 aaatccattt agataatata aatgtgatgg gtatcgtaat aattaaacca gcaaatggtg
      661 caatttctgc tggcaaattt agccaggata caaatacata taataaaact gtttgtaagc
      721 ttatgttgac aatctgcgta attggaaaac taatgaattt tctccaagta ggttttaccc
      781 tgtaaacaaa ataacaattc aaataatacg aaatcacaaa agcgactata aatccggtaa
      841 tatgactaat catatattca atgcgtaata attttaacag caataaatag acaacataat
      901 agtttaacgt attaatgccg ccaacaatga taaattttaa aatttcagca tgcgtttgtg
      961 ttagtttcat atgtgtaact cctcaacatc aaaatatatg tataactgcg ttctccaaca
     1021 tactcgaata tgcgagccaa tccgcttctc ttcaaatata cttatttcag tttttatacc
     1081 ctttcacagc aaatttagtc tctttcccct catccttata cgccattata atgtaactga
     1141 tttatcgcgc gactcattag cactatagag attactttag ttcactagta attttatata
     1201 caataagagc gacaacagta atgagaggat gtctactatg caattacaaa aaattgtcat
     1261 cgctcctgac tcatttaagg aaagtatgac cgcacagcaa gttggcaata ttataaaaca
     1321 ggcatttact aatgtttatg ggaaaaccct tcattatgat atcattccga tggctgatgg
     1381 tggtgaaggc accacagatg ctttaatgca tgcaacaggt gctactaagt atacagtcat
     1441 cgttaatgac cctttaatgc gacctattga agcatgttat gcacgcgcag acgaacaaca
     1501 aattgcaatt attgaaatgg cagcagcgtc aggtttggat ttattagaaa aagaggaacg
     1561 taatccttta tacacatcat catatggtac cggtgaacta attaaagatg cattaaatca
     1621 tggtgctaag accattattt tagggattgg tggcagtgca acaaatgatg gtggtacagg
     1681 tatgctaagt gcactaggcg taaagtttac tgatgtaaac ggggacttat tacaaatgaa
     1741 tggtgataat cttgctcaca ttgcacaaat cgatataacc aatctagatt cgcgattaaa
     1801 agaagtgacc tttaaagtgg cctgcgatgt ttcaaatcct ttattgggtg aaaatggtgc
     1861 tacctatatt tatggtcctc aaaaaggcgc tgatgcaaag atgataccaa agttggattt
     1921 cgcaatgtcg cattatcatg ataagataaa aatgtgcaca ggaaagtcag ttaatcaaat
     1981 accaggttct ggtgcagctg gcggtatggg cgcagcatta ttagcgtttt gtgagacaac
     2041 tttaacaaaa ggtattgatg tcgtctttga cattacagat tttcatcaaa gaattaaaga
     2101 tgcagacctc gttattactg gagaaggacg catggattat cagaccatct ttggtaaaac
     2161 acccgtaggc gttgcgttag ctgcaaaaca atatcatatt cctgtcatcg cgatttgtgg
     2221 cagtctaggc gaaaattatc aacatgttta cgatttcggt attgatagtg cctattctat
     2281 aatctcttca cctagcactt tagaagatgt cctacaaaat agcgaacaaa atttattaaa
     2341 cactgcaact gacattgctc gtattctgaa attacaataa tgtcaaagta aatcatcagc
     2401 tttattattt gcagttaaaa cttgaatgag gtgaaaccca tgaaaagaac tgataaatac
     2461 cgtgattcat atcaatacga caatcaaaat caaaatcatc gtcgtcaatc tgaagacgca
     2521 tcgtatagac aacaatatgc taaaggtgat cctgaagaac acccagaacg atactataac
     2581 ggtagagatt atcgaagaga acaaattctt gaagaagaaa acgagaaatc ccgccgttca
     2641 aaaaaatggt tatatatcat tattgccatt ctcttaatta ttgtcgctat ttttgtcaca
     2701 cgcgccttac ttaacaatga tagcgataaa gttagtaatg accctaaagt ctctcaaaat
     2761 tataaaaaac aagttgaaaa tcaagacggc caaattaacc agcaagtcga taatgctaaa
     2821 gaaaatatta aaaacaacca aaaaactgat gacattatta aaaatttaca aaatcaaatc
     2881 gataacttga agcagcaaga acaaaacaaa gctgattcta agctaactca attttatcaa
     2941 gaccaaatca acaaattgac agaggcaaat aatgcactta aaaacaatgc aagccaaggt
     3001 aaaattgaaa gcatgttaaa tgatattaat acaaaattcg acagtattaa atctaaatta
     3061 gaaagcttat ttaaagatga caatggtggc gctaattaat tattaaacct gcttttgatg
     3121 ataaacatta attccctata ctttatctgt atcactacgt tattcgtgat gatgcattaa
     3181 gagtataggg attttttata taaacttgta ttctaactac atacaaatac acacaaaacg
     3241 tatataattt atataattat ataaattaag gagtctgttt atgcaatctt cgaaaaccaa
     3301 aacgaagcat ttttcatttt tattgctaat cacgttaggt gtcatgaccg cttttggccc
     3361 tctaactata gatatgtacg taccatcatt acctaaagtg caaggtgatt ttggttctac
     3421 tacatcagaa attcaattaa cattatcatt cacaatgatt ggtcttgcac taggccaatt
     3481 tatctttgga cctttatccg atgcttttgg tcgcaaacgt attgctgtat ccattttaat
     3541 cattttcatt ttggcatcag gtttgtctat gtttgttgat caattgccat tattcttaac
     3601 tttacgattt attcaaggtt tgacaggtgg tggcgtcatc gttattgcca aagcctctgc
     3661 tggtgataaa tttagtggca acgcactcgc taaattttta gcatctttaa tggtagttaa
     3721 tggcatcatc actattcttg caccattagc cggtggatta gctttatccg tagcaacatg
     3781 gcgttctatt tttacaattt taacaattgt ggcactcatc attttaattg gcgtcgcttc
     3841 tcaattacct aaaacaccta aagaagaatt aaagcaggtg aattttagta gcgtcattaa
     3901 agactttgga agtcttttga aaaaaccagc atttattatt ccaatgctat tacaaggttt
     3961 aacttatgta atgctattta gttattcatc tgcatcgcca tttattactc aaaaattgta
     4021 taatatgaca ccccaacaat ttagtatcat gtttgctgtt aacggtgtag gtttaatcat
     4081 tgtcagtcaa gtcgttgctt tattagtaga aaaattacat cgtcacatat tattaatcat
     4141 tttaactatt atacaagtgg taggtgttgc tttaattatc ctgacactta cattccattt
     4201 accactttgg gtcttactca tcgcattctt cttaaatgtg tgtcctgtga cgtcaattgg
     4261 accgcttggt ttcacaatgg ctatggaaga acgaacaggt ggcagtggta acgcatcaag
     4321 tttacttggc ttattccaat ttatcttagg tggcgctgtt gcaccattag ttggcttaaa
     4381 aggcgaattt aatacatcac catatatgat tattatcttc attacagcca ttctattagt
     4441 cagtctacaa atcatttact ttaaaatgat taaaaagcaa catgtcgcat aacacttcaa
     4501 cataattaga accctagcaa agatatctat ctttgtcagg gttcttcttt atgaattatg
     4561 agattgaatc ttcaactaaa attacgcctt catatcaagt acatttctat tcaatcaccc
     4621 tttaacaggc atccaaattt ctgtaatata tttttcactt gtagtatcac catcctgata
     4681 caactcaaaa aatggtgctg attttaccgt atctgcttga tattgatgta tgtagtgatg
     4741 aacctcttcc attttttgtt gaactgcttt aggtactgct ccctgtgctt caaataccat
     4801 atactttgaa cttgctaatg ttatgacatc atatttttca atatcagcac tattatctcc
     4861 ggtaactgca atcgtatatg acatcttacc atctaattca ggtatacata agccaagtat
     4921 cccggctaaa tcaccattat tttttaactg taaatctgca attactccct cttgataaca
     4981 tctttgccaa aaccctgcta tatgttgctg agcctgtcgt ccattttcat acgtttcttt
     5041 aatacctact aacgttaacg acgcaagttg ttgtaattga tattccattt tatcactcca
     5101 ttaattttat tcactcatgt aaaacatatg tttaggacat tttgcattga cgctatataa
     5161 ttatattaaa tatttattga atcaatgcga ggactatatt atgaaccgaa aaatattacc
     5221 agttaaacct atcagtcagc tatttcctat tcctatggtc atggggtgtg aaggtgtttc
     5281 cgcagccatg atgttgcaat acaataacca acatatccct gctacagaaa ttatgagaca
     5341 ttggccgaca catccgaaca atccacataa aggttatgtt ggacatcact tattcattaa
     5401 gcttggcaat tatcatcaaa caatatttcc agatgcatat gtaccatttt tacaaaaata
     5461 caatcctaat atcgtagacg gcactggtaa atccttagaa gatcttaaag gtattatcga
     5521 tcaaggtcaa cctgtgttaa tataccatac taatcttggt tctaaaccat tactacgtgt
     5581 tttccgcttt gataacaagc cagcgaaaca agtttcaaat attcacgtaa cgttactcat
     5641 tgggtacgat gattactatt actattatat cgatccactt tggagtcata ttcgtcgtgg
     5701 cctagtttta cctgccatca ttccaaatcg caaacaaatt attaaaattc gtaaagagaa
     5761 aatggaatac agctttaatt caccaggaag aaagtgcatt tatgtgcaac cacattcata
     5821 tacaattgaa aatcagaaac aaaataaaca cacgtaagta aacacattac tcgcgtgtgt
     5881 aaaacataaa atgcgcaaac tgggtcaggt ttgcgcattt taaatttcta ttatttgtat
     5941 cgtctcattt atttcctggt aaatatcttc tgaacgccat tgtcttggat agcctagaca
     6001 cggacagaga taacatctgc catcatccat cacacttttc cgaaaatgaa catggcccat
     6061 aatactatat ggtatatcga acatcgcata caaaggattg aaatcacttg tcccaataaa
     6121 tgcattataa aaatcgaata tacgatgcgg cataggaaca atgaaatcag ggtgcgtcac
     6181 aacatgtgtg actaaaatca ctcttctatt tcctacttct aatagatctt tcttcacttg
     6241 ttcagccgct attttagata aattttgatc tgatgtgccc caagatattc gttctttatc
     6301 ttgccaagtc gcaccataat gttttcctct ttgtaa
//
LOCUS       DAMZPP010000148         6195 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMZPP010000148 DAMZPP010000000
VERSION     DAMZPP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.148"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(77..392)
                     /locus_tag="POH73_002387"
                     /pseudo
     CDS             complement(77..392)
                     /locus_tag="POH73_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            complement(385..1313)
                     /locus_tag="POH73_002388"
                     /pseudo
     CDS             complement(385..1313)
                     /locus_tag="POH73_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            1600..2343
                     /locus_tag="POH73_002389"
     CDS             1600..2343
                     /locus_tag="POH73_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDI6888306.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKREDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            2893..2988
                     /locus_tag="POH73_002390"
     CDS             2893..2988
                     /locus_tag="POH73_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDI6888307.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGYWLHKRDKK"
     gene            complement(3203..3544)
                     /locus_tag="POH73_002391"
     CDS             complement(3203..3544)
                     /locus_tag="POH73_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6888308.1"
                     /translation="MKLAEAIKEQRELKRWSQEELANILKVSRQSVSKWESAKNYPSL
                     DILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRAPSIVDFLYDFWWLFFPVAG
                     FLVWAIHSFMG"
     gene            complement(3631..4491)
                     /locus_tag="POH73_002392"
     CDS             complement(3631..4491)
                     /locus_tag="POH73_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDI6888309.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLR
                     LSFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIH
                     KREQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLK
                     IHIDDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEF
                     TFDAEKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKERED
                     FLKQLELNWE"
     gene            complement(4856..5356)
                     /locus_tag="POH73_002393"
     CDS             complement(4856..5356)
                     /locus_tag="POH73_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDI6888310.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTYFEKYTKQIKAAQMIL
                     DDYEWIKSGKSLKKSEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKD
                     KELNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKK
                     KYQAQE"
     gene            complement(5423..5749)
                     /locus_tag="POH73_002394"
     CDS             complement(5423..5749)
                     /locus_tag="POH73_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDI6888311.1"
                     /translation="MEEEYGKENLLYATVHMDEITPHMHYGVIPITKDGRLSAKEVVG
                     NKKALTEFQDRFNTYINKQGYDLKRGISRQLTKEKHDQVSGYKQKTEYHKQMYMREKQ
                     IEDHLK"
     gene            complement(5689..6066)
                     /locus_tag="POH73_002395"
     CDS             complement(5689..6066)
                     /locus_tag="POH73_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDI6888312.1"
                     /translation="MSYSIIRVVKVKSKTNTRGIQRENKNYENIDIDLSKSYLNYDLV
                     NDTKFDFNKKIDEKIEKNYKGKRKIRTDAIKHIDGLITSDNVFLINYLKKRQNSFLSM
                     LKRSWKKNTVKKIFYMRQYIWMR"
BASE COUNT         2051 a          974 c          790 g         2380 t
ORIGIN      
        1 ctacatacag catttgtcac aaaaataaga cagcactttt ttacagaaaa acaataaaaa
       61 aagacatcag ttatcactaa tgtcttaaat aataattttg cttttcgaaa ttccaaattg
      121 cggtaattga atgtgctttt tttcttcgtc tgtcgtttta tcttcatcac ttgtattaat
      181 caaattacca tcttcggcat cgtctaagtt taaaatttta tgcttttgtt taagcaatcc
      241 accataactt aacatacgtt ttcgatacaa acctttttct aaatcactta caatttcttg
      301 attttttcgt cgtcatcagt taaaaagtca gatgatttaa cggaatattt tgatgtctct
      361 tcgagtgtta taaaatcatt tgaattattg attaaagata ttatattgtt tcatgttttt
      421 acggcgcata taaagggcat gtgtttcttc aagttcagtt agaattgttt gcattaaata
      481 agcttgcatt gactttattg tttccttttg gcctttaact cccctatcat gtatagcttt
      541 tttaagtctt tttaaagaag ttaatatttc atcctcaatg tcctctaaca tgtaatgcat
      601 atcttcatgc cttgaattaa atgatttttt tgctttaaga ataacagcct ttatgatttc
      661 taaatctcta taatcatatt ttaataaata attagttagt tgttctggga attcttgcaa
      721 tagcatttct ttatcatcaa aattatgtgt aaattgattt gaatgattac taatttcatt
      781 tttgtacttt atatcattca agtcattcat atcattattc tcagtatcat taaagtcaat
      841 gtcattaaag tcagtatcat tagtgttcga ttttcgaact tctggacgtt cgattttcga
      901 acttccttct cgccgtaaag ttcaacatcg ttttcttctt tttctatttt gtaaatatca
      961 cgttctgtga caataggttt cattaaatat agaatatttg gtttatttaa accacgtcgt
     1021 ttttgaatga gtagaccatc gttttctaac ccttttttta tttttgttaa cttttctttt
     1081 ttacaattta agatttccat tagcttttca tttgaataaa caaagtaaat gttaccatct
     1141 tcatcaaccc agttattttt aatagataaa tttaagcgat cacgtaagat agcataggct
     1201 attttcatat cattagttag atttttataa ttttcactgg taaagaatac tttagggatt
     1261 tgataaaatc tttctttgta attttcttgc actgtaaaat attgatttta catgacaaaa
     1321 aacctctttc catattttag tttggtaaga ggtaataaaa cgtttttatt tagctttaca
     1381 tttatttaat gatttaggtg cttgcactaa gatattattc attatataat acaagtatcg
     1441 aaaattatat aaataaacag tttctaaacg ttttattacc aaaccccgac tattaccagt
     1501 agttggggtt tttgttatat taagtttatt atagcacttt aaataaactt ataaaaggga
     1561 aaatatagtt ttatcaaggg tttataaagg gttgatgata tgaaaagtgt gaaaaagttg
     1621 agtgaagaat taggggttag taagcaaacg atttttaata atattaagcg attgaatata
     1681 gagacaataa aacaggaaaa cacttcgttt attaaagaag ataccgatat agaaaaaatc
     1741 attcaaagag taaatgaaaa taaaaagaaa tatggttttg aaagtactac tgaagacaaa
     1801 caaaaaaaag ggtcggataa tattaatttt gaatcgaaaa gcgatgctca aatagtagaa
     1861 atccttaaaa atcagattga tactttaaat aatcaaattg aaaaacaaga aagtcgtcat
     1921 gaaacaacaa ttgaatttta cagaaaagag ttgcaagaaa gatcaaaatt gcttgaaaat
     1981 cagcaagttc tggcattaga gagtaataaa aagattcaaa aattagaaaa tcaattagaa
     2041 aaagaaaaac accttaatta tccctttgat acatctacta acactagaca aaacgttgat
     2101 gcacaagaaa aaacatatac tactaatcct gtaaatatta acagagatca ggaagaaacc
     2161 aaagaaacag agattcaata taaggatata tctggaagtc aaagcgacga aagtacacaa
     2221 ggtgaagaag caaaaagaga agacgttcca gctaatctaa atgacaatga tagtgatata
     2281 gaagaaaaga gtgaagaaac ggaacctaaa aaggggtttt ggagtcgttt gtttggtagc
     2341 taaaataatt gattaggcac taaaagttat aaaattttag tgtcttttat atagtgagaa
     2401 cttctggtaa tcatcgatta tgttaaccaa acaaaaagcc ataaatgccg tcatataggc
     2461 atttatggct ttttactttc attatctttt atgcatcttt tatacatcgt tgatttgacg
     2521 cgttttcatt cttttgcatt ggcaagtatt ttgtatcttt attcagttac tatttctttt
     2581 ctccaaccta acaaaccatc tttaactcta ataatatgtt tgccacgcca ggcacttctt
     2641 acttcgaaat aataccattc cccgtttttg ttgaggaaat aaatatcttg ataaccttct
     2701 ttaatgacat attttgtagc ttcttatctt tgatcgccaa acattatcaa tcccaccctt
     2761 atatttattt atctttaata tataacaaca tttattaata ttcatgttat ttcatggaag
     2821 aagagatttg ttataatgtg aaggtcgtct atctctcaga cgtcaattcg gcgtagagag
     2881 gaggtgcgat ttatgaatga aattcttgtt cacatcatga caacggcaat cagtggttgt
     2941 ctcgttacgt tatttggtta ttggctccat aaacgtgata aaaaataata tagacgatta
     3001 aagccaattc accaaaaaac cccgaactac ggcgaatagt tcggggtttg gtgcgtttac
     3061 aaacgaaatt cttgttactt ttagtatata tttcatattg ccttttgtaa actcttctat
     3121 tcttatcaat acatttcaag gtgtcgtaaa aatactttcc tttttcatat tgcctgatgt
     3181 gcattaaatt ttgattttaa aatcaaccca tgaacgaatg aatggcccaa actaaaaatc
     3241 cggcaacagg aaagaaaagc caccaaaaat cataaagaaa atcaactatg gaaggtgccc
     3301 tgttactctc tctataattt ccctctaaaa taactttttt aaaacgtgca tctcctttaa
     3361 ttaaatgctc taaagagatt ccaaataaat cgctcatagc aatcaaaata tctaaggaag
     3421 gatagttttt ggcactttcc catttagaaa cactttgtct ggaaaccttt aatatattgg
     3481 ctaattcctc ttgagaccat ctttttaatt ctctctgttc ttttatagct tcagctaatt
     3541 tcataaaatc actccttaac tcaaactata attaaaataa ttgaaaagtc acgatacttt
     3601 tagttaacca actcgcaacg aaaagttgcg ttattcccaa ttcagttcaa gttgttttaa
     3661 aaaatcctct cgttctttct ctaattgtgg gtcgtaatct ttttgaggtt gcttttcata
     3721 tgaccgttct tctaaccatt taggcgtctt ttcacgcctc acatattgtc ttgatttatc
     3781 tatcttggac atttgaggtt gtcgtttgtt atgaatgcgt ttctcagcgt caaatgtgaa
     3841 ctctatccat tcaattttac gccctttttt cgctttgatt ttattgatag taagattatt
     3901 aaatatagaa cctaattcaa taataatagg tttaaaaaca tttttgttaa tatcagtcat
     3961 acgataagat ttaggtatat ctaaacgatt tttaaaatca tcgatgtgaa tttttaaata
     4021 gcctgtatgt ttgtattgtt taagtaatct aaacatattt ttagaatatg tagatttaag
     4081 cctagtcatt tcttttaatt caaattttgt aaaattatta gtaattgaat tcaatatgtg
     4141 ttttaaattt gaagaagttg atatttctag atattgttct cgcttatgga ttctataatg
     4201 attgaataaa acgaatcttt ctattacatc atcatcttca tatctaatgg ttaaattcaa
     4261 catcttttta tacacattat ctaaatcatt agcaaatcta ttaatattac gtgtttctgg
     4321 actataatta cttaattttt ttaattcatc aaaagacaaa cgtaatgtat tagtatcttg
     4381 ctcttttaat ttattacaca tcgcaaaaaa taaattaatt tcagtagctg taaatcgcct
     4441 taatggaact aaattcattt catttttata tacaactgtc tctcctgtca tgatacagcc
     4501 ccccctttat tacgaaccta aaggagtatc gtttgaatat acactcccat ttgaaaactt
     4561 ttattcacaa taaactactt tattatcaat gtcaaaccat aaaaggagta caacttttta
     4621 aaacactcct tttatgtggc aaaaacactc ctttaggcat tgaaatccct tgttgatcta
     4681 ggtctgaaca agcccctaaa aaggttttta aaaagattta taaaaatata ttaaaaagtt
     4741 agaggggggc tacctatttt tcatcctaaa aaataattaa cctcaaacaa atgcatttaa
     4801 atatctattt ttataatgta taaccatctt ctctatcttc tttaaaaatg atttctcatt
     4861 cttgtgcttg atatttcttc ttaaagaact gttcatcatt gttatctatc gtcattactt
     4921 cacgtatttt agaattgtct aaatgattat caatatgatt tattagcgta tgataacgct
     4981 cttctttcat catactttta atgattttaa aaacttgatt gaataactta agcgtataat
     5041 ttagttcttt atctttcttt tcattctctg acaacaaata attataacgt tgtgctaaat
     5101 cattaaattc ttcaaccagc ttttcatttt ccatatggac actagtcaat tcattaacta
     5161 aactttcatt ttgtttctct gattttttta atgacttccc acttttaatc cattcataat
     5221 catctaatat catctgtgct gctttaattt gtttagtgta tttttcaaaa taagttttag
     5281 taattacaat attgcctgtt tcttgcttct ctttatgaaa ccatccacct acatttttag
     5341 ttttaatttc atacatgaca ctcagaagct ttttaagcgt atctaatgat ttttggtagt
     5401 tttatatcaa tttatcattt tctcacttta aatgatcctc tatttgcttc tcgcgcatat
     5461 acatttgttt atgatattct gttttttgtt tatagccgct aacttgatca tgtttttctt
     5521 ttgtaagttg tctagaaatt cctcgtttta aatcatagcc ttgcttatta atatacgtat
     5581 tgaatctatc ttggaattcg gttaatgctt ttttattacc tacaacttct ttcgcactta
     5641 aacgtccatc tttagtaata ggaattacgc cataatgcat atgtggtgtt atctcatcca
     5701 tatgtactgt cgcatataaa agattttctt taccgtattc ttcttccaag aacgctttag
     5761 catgcttaaa aaactatttt gtctcttctt cagataattg attaaaaaca cattatctga
     5821 tgtaattaat ccatcaatat gtttaatggc atctgttcta attttgcgtt tccctttata
     5881 atttttttca attttctcat caattttttt attaaaatca aacttagtat cattgactaa
     5941 atcatagttt aaatatgact tacttaaatc aatatctata ttttcataat ttttattttc
     6001 tctttgaata cctcttgtat ttgtttttga tttcacttta acaactcgaa taattgaata
     6061 agacataata aatcaacttc cactgcatat gattataaca cctaaacaca gaaaagtttt
     6121 aacatactag acttattaaa aaccaaataa gtcattaaaa acgtgtgctc tgcgaggctt
     6181 gttttttgtc atcac
//
LOCUS       DAMZPP010000149         6086 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMZPP010000149 DAMZPP010000000
VERSION     DAMZPP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.149"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..616)
                     /locus_tag="POH73_002396"
     CDS             complement(<1..616)
                     /locus_tag="POH73_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDI6888313.1"
                     /translation="MGLKDQAVAWRRYFHQFPELSDKEFKTTQKIKDILTEYNIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQPAEELGHGAFKIIETHALQDVQAVLGFHNDPSRPVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLIN"
     gene            complement(891..2129)
                     /locus_tag="POH73_002397"
     CDS             complement(891..2129)
                     /locus_tag="POH73_002397"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HDI6888314.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKHYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNIQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTLSFKV"
     gene            complement(2129..3790)
                     /gene="hutU"
                     /locus_tag="POH73_002398"
     CDS             complement(2129..3790)
                     /gene="hutU"
                     /locus_tag="POH73_002398"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HDI6888315.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDTEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIATKYCDIKTANLDEALKLA
                     EEAKERGEGLSIGLVGNAVDLHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFHKRGAVAFDYGNNIRQVAFNNGVRNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            3950..4834
                     /locus_tag="POH73_002399"
     CDS             3950..4834
                     /locus_tag="POH73_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6888316.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLCRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            5076..5495
                     /gene="fosB"
                     /locus_tag="POH73_002400"
     CDS             5076..5495
                     /gene="fosB"
                     /locus_tag="POH73_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB/FosD family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HDI6888317.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFKLAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(5751..>6086)
                     /locus_tag="POH73_002401"
     CDS             complement(5751..>6086)
                     /locus_tag="POH73_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HDI6888318.1"
                     /translation="YAKEKKIDYVFADELLSHVSPTIKDMIERFIHEHDVIMFTICMD
                     VIDSAFAPGVSAPAVLGLYPHTVLELAKRIIPSDKVSSVSIAEMNPTYDADNRTAKLV
                     ANLVHHFLK"
BASE COUNT         1945 a         1264 c          860 g         2017 t
ORIGIN      
        1 tattaatcaa ttgcgccaac acaataactg gatcgttgca ttgttctggc tttgcagcat
       61 gaccacccac gcctttaata tgaaactcaa aacgatctac tgctgatgta attgcccctg
      121 ttttgattgc aaatgtacct accggacgcg atgggtcatt atgaaaaccc aatactgctt
      181 gtacatcttg taatgcatgt gtttcaataa ttttaaaagc gccatgtcct agttcttctg
      241 ctggttgaaa aatgaattta acacgcccag taagagtgcc ctcaatttct tttaacttta
      301 cagctgtagc caaaatacta gccatgtgaa tatcatgacc acacgcatgc atcacacctt
      361 cattttcaga tttaaaatcc tgttcaacaa gctcttgaat tggtaaagca tcaatgtcag
      421 cccgaacagc tatacaactc agaccttgcc ccacttcagc aacaagtcca gtcgcaagtg
      481 gtaagtctaa tattctaata ttatattctg ttaaaatatc tttaattttt tgtgtagtct
      541 taaattcttt atcggatagt tctggaaatt gatgaaaata ccttctccag gcaacagctt
      601 gatcttttaa tcccatcggt cattcccctt ccttaagtca atgatatgtt gtctacccta
      661 cgatgatcgt ctttaactat taaacgatga tttcacaaca atgtactctt gttaattact
      721 ttcgctaatg atagacagtt gtttaataat atcgtaacat tattgtcaaa ctattctaac
      781 ttttataatt aagaacctat acaaaaacgt gttctcagat aaacttgttt tacaaaccac
      841 aaaaagctct aaacattagt gtaaaccaat gcttagagct tttaattact ttatacttta
      901 aaagataatg tgttatctac gatgacctta ccgtctttaa taactttttc tgcgtgattg
      961 ataccaaaat gataaggaat atattcatga tttggtgcat cccaaattac taaattagcc
     1021 ttatcacctg tgttaattgt acccgcgtta atgtctattg ctttagcagc attgaccgta
     1081 acagcattcc aaacttcatt aggcgatagc tttaatttca acgctgcaat cgccataaca
     1141 agctgtatgt tgtttgtgac actacttccc ggattataat cagttgctaa tgcaatcgca
     1201 ccgttattgt caagcatgcc tcttgcatct gcataatctt ctttacctaa atagaacgtc
     1261 gttgcaggta agaggacagc tacagtatca ctatttcgca acttttcttt tcctttgtca
     1321 ctagacgcta ctaagtggtc agctgatatt gcttgttcgt caattgctaa ttccagtccg
     1381 cctaatggat caatttcatc cgcatgtatt ttcactttaa aacctgcttc tttggctttt
     1441 tgcatataat gttgcgattg ctctattgta aatacacctg tttcacagaa tatatccgca
     1501 aagtctgcat aatgttttac ttccggaagt aacgcaatca tttcttctaa aaatgcctca
     1561 tttgaacttg cctctttagg tacagcatga ggccctagga aagtatgttt catatctaaa
     1621 tcatatttct cagctaaacg attagacact ttcaattgct tcagttcatt ttctctatct
     1681 aatccataac cactcttact ttcaactgca agcacgccgt gtttaatcat agtaagcaaa
     1741 tcatgctctg cttttttaaa caagtcatct tcggatgttt ctctagtagc attaacggta
     1801 gataatatgc caccacccat ttctaatatt tcaaggtaag acttaccttg acgttttaat
     1861 gacatctcat gttctcgaga tccaccaaat gttaaatggg tatgagcatc tactaatgct
     1921 ggggacacta ccttcccact agcatcaatt gtctcagtcg catcgtagtc atctgtatgt
     1981 gttccagcat atacaatttt gccatcttta atgacaactg taccattttt cacaacattt
     2041 aattcatcta attccttacc cttcaaaggt ttatctgttg atctcggtaa aattaattct
     2101 gctatatgat taattattaa atcattcatt acttatcacc tgctttatca atcattggaa
     2161 tatgaatacc tttttcttta gctgtttgaa tagcaatgtc atagccagca tcaacatgtc
     2221 gtgcaacacc catacctgga tcagtcgtca atacgcgttc caatcttctt tcagcacgct
     2281 ctgatccatc tgctacgaca accatacccg catgaagtga atatcccatg ccaacaccgc
     2341 caccatgatg gaatgaaatc catgaaccac ctgcagccgt gttaatgagt gcattcaata
     2401 cagcccaatc accaaccgcg tcactaccat ctttcatact ttctgtttca cggttaggac
     2461 tcgcaactga accagcatct aaatggtctc gtccaataac aattggtgct gaaatttcac
     2521 cgtcacgtac aagacgattt aaagctaagc ccatttttgc tctttctcca tagcctaacc
     2581 aagcaatacg tgatggtagt ccttgatatg aaattttttc ttcagctaaa tcaagccatc
     2641 ttaataactt ttcattttct gggaaaagtt tacgcatttc ttcatccgca cgctcgatat
     2701 cttttggatc accactcaat gcagcaaagc ggaatggccc cttaccttca cagaataatg
     2761 gtctaatgta agctggtaca aagcctggga agtcaaaagc atttctcact ccgttattga
     2821 aggctacttg acgaatattg ttaccataat caaatgctac agctccacgt ttatggaatt
     2881 caagcattaa ttcaacatgc tttgccattg aagcttgtga cagttcaaca tattttttcg
     2941 gatctttttc acgcaatact ttcgcttctt ctacggagta tccttgtggc acatatccat
     3001 ttagcggatc atgtgcactt gtttggtcag taataatgtc aattttaaat cctttttcta
     3061 gaatcgcttg atggaggtct acagcatttc caactaaccc gattgacaat ccttctccac
     3121 gttctttcgc ttcttctgct aattttaatg cttcatctaa attagctgtt ttaatatcac
     3181 aatatttcgt agcaattcgc ttatcaacac gtgtttcatc aacatccaca caaattgcta
     3241 ccccatgatt catagtaatt gctaacggtt gcgcaccacc cataccacct aaacctgctg
     3301 tcagtgtaac agtgcctgct aaatctccat taaagtgttg attacctagc tcagcaaatg
     3361 tctcataagt accttgcaca ataccttgag aaccaatata tatccaacta ccggctgtca
     3421 tctgtccata catgattaaa ccttttttat ctaattcatt aaaatgatcc cagtttgccc
     3481 attcaggcac taatactgaa tttgaaatta atacacgtgg cgcttcttca tgtgttttaa
     3541 atacagcaac tggctttcct gattgtacta acattgtctc atctgattct aattcgcgta
     3601 acgttttctc tattgcttca aaagcttccc aattacgagc tgcttttcca ataccaccat
     3661 aaacaactaa atcttctggt ctttcagcaa cttctgtgtc taaattgttg tataacattc
     3721 taagtactgc ttcttgttcc caacctttac actcaatact caaacctttt tttgcttgaa
     3781 tttttctcat aaaattcgct cctgttcttt ttagaagtta attccactaa atttaaaacg
     3841 cttacattat tatcttcaat attcattata gtatgttaaa atatagccaa caaatataaa
     3901 taaactaatt atccatagct tgaatctata aataaaagga gcaaaaaaca tgaaaattat
     3961 tcagttagaa tacttcttgg ctatcgtgaa atataatagt tttactaaag ctgcacaatt
     4021 tttacatatt agccagccat ctttaactgc tacgattaaa aaaatggaag cagatttagg
     4081 ttatgactta tttacacgtt caacaaaaga catcaagatt accgaaaaag gaatacagtt
     4141 ttatcgttat gcgagcgaat tagttcaaca atatcgatcc acgatggaaa aaatgtatga
     4201 tttaagcgtt acatcagaac caaggataaa aattgggact cttgaatcta cgaatcaatg
     4261 gattgcgaat ttaattcgaa agcaccattc cgactaccct gaacagcaat atcgtttata
     4321 tgaaatacat gacaaacatc aatctataga gcaattactg aattttaata ttcatttagc
     4381 tataacaaat gaaaaaataa cccacgaaga tataagatcc attcctttat atgaggaatc
     4441 ttacatttta ttagcaccta aagaaacatt taaaaatcaa aattgggtag atgttgaaaa
     4501 tttgccactc atattgccaa acaaaaattc tcaagtgcgc aaacacttag atgactattt
     4561 taatagaaga aatattcgtc caaatgtcgt tgtagaaaca gatcgattcg aatcagcagt
     4621 tggatttgtt catcttggct taggttacgc tatcattccg agattttatt accaatcatt
     4681 tcacacgtcc aatttagaat ataaaaaaat tcgtccaaac ttatgccgaa aaatttatat
     4741 caattatcat aaaaagcgta aacactccga acaagtacat acattcgtac aacaatgcca
     4801 agattattta tatggacttt tagaggctct ttaacttaag ttattagagc ctcttatgca
     4861 gttgctcagt caactgtata ccttttgcct ttaacttaag ttattagagc ctcttatgca
     4921 gctgctcagt caactgtata ccttttgcct ttaacttaag ttattagagc ctcttatgca
     4981 gttgctcagt caactgtata aaaattaatg acgtcatttc aaaaatcgat acaaaaataa
     5041 tttattataa aaattctaag aaagaagtga acccgatgtt aaaatctatt aatcatatat
     5101 gcttttcagt cagaaattta aacgattcaa tacattttta tagagatatt ttacttggaa
     5161 aattgctatt gactggtaaa aaaactgctt attttaagct tgcaggccta tggattgctt
     5221 taaatgaaga aaaagatata ccacgtaatg aaatccactt ttcatataca catatagctt
     5281 tcactataga tgacagcgaa tttaaatatt ggcatcagag gttaaaagat aataacgtga
     5341 atatcttaga aggaagagtt agagatatta gagatagaca atcaatttac tttactgacc
     5401 ctgatggtca taagctagaa ttacatactg gcacacttga gaacagatta aattattata
     5461 aagaggctaa accacatatg acattttaca aataaggcgt cattataaaa aggcctcttg
     5521 aactccgtta aaattttaat taattattat ataataagag aacttttcaa acaatacagt
     5581 tgttattttt actatctcaa caaacataaa taagcagtaa gatgactaca acttaagagt
     5641 cttcttactg caattatctt tcaaatatat caacgttaat ataacttcta ttacgaaata
     5701 ctcacattct gccctgcaat gcaaatttcg tcacatataa atatttttaa ttattttaaa
     5761 aaatgatgca ctaaattagc aacgagcttc gcagttctat tgtcagcgtc atatgttgga
     5821 ttcatctcag caatactaac tgaagacacc ttatcacttg gaataatacg ttttgccaat
     5881 tcaagaacag tatgtggata caaacctaac actgccggcg cacttacccc aggcgcaaat
     5941 gcactatcaa tgacatccat acaaatcgta aacataatga catcatgttc atgtataaaa
     6001 cgctcaatca tatctttgat tgttggtgat acgtgactca ataattcatc tgcaaagaca
     6061 taatcaatct ttttctcttt agcata
//
LOCUS       DAMZPP010000150         6021 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMZPP010000150 DAMZPP010000000
VERSION     DAMZPP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.150"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            406..3111
                     /gene="acnA"
                     /locus_tag="POH73_002402"
     CDS             406..3111
                     /gene="acnA"
                     /locus_tag="POH73_002402"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDI6888319.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSKLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRGAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGSVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            3292..3759
                     /locus_tag="POH73_002403"
     CDS             3292..3759
                     /locus_tag="POH73_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDI6888320.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQALNNEGKIVEIMDGIDSL"
     gene            complement(3968..4264)
                     /locus_tag="POH73_002404"
     CDS             complement(3968..4264)
                     /locus_tag="POH73_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HDI6888321.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSKK"
     gene            complement(4642..5250)
                     /gene="plsY"
                     /locus_tag="POH73_002405"
     CDS             complement(4642..5250)
                     /gene="plsY"
                     /locus_tag="POH73_002405"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDI6888322.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLPVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5456..>6021
                     /locus_tag="POH73_002406"
     CDS             5456..>6021
                     /locus_tag="POH73_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDI6888323.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVL"
BASE COUNT         2099 a          907 c         1117 g         1898 t
ORIGIN      
        1 ataggtttat ttatgacaac ttaatagtaa ggggtttgac aaagctaaag tgatgtacga
       61 cggtataaac aaaaattaac taaacatatt tcggtatgtg tatatcaaaa gaatgacatt
      121 gaaaagaaat tttaaagttt gacgcaataa ttttctttct gctcaatgaa ataatacgtt
      181 taaaaattga attgaatcat tcacgaaatt gataattatt ctcaattaat aaaaaatact
      241 tattacacct tacctgcatg ataagtttta cttatatact ctgattaaaa agtcaaaacc
      301 taaattaagc gttttctaag catgatgagt tgtttatcaa taaatataat aatacaattt
      361 aatcaaggca tataaatata aaaatgtatc aagggggatc attaaatggc tgcaaatttt
      421 aaagagcaat caaaaaaaca ttttgacttg aatggccaaa gttatactta ctatgattta
      481 aaagctgtag aagagcaagg tattactaaa gtttcgaaat taccttattc aattcgtgtt
      541 ttgttagaat ctttacttcg tcaagaagat gattttgtaa ttacagacga tcatattaaa
      601 gctttaagtc agtttggaaa agatggaaat gaaggcgagg taccatttaa accttctcgt
      661 gttattttac aagatttcac aggtgtacca gccgtagttg atttagcttc tttacgtaaa
      721 gcaatggatg acgttggggg agatattact aaaattaatc cagaagtacc ggtggattta
      781 gttattgacc actcagttca agtggatagc tatgcaaacc cagaagcact tgaacgtaat
      841 atgaaattag aatttgaacg taactatgaa cgttatcagt ttttaaattg ggcaacgaaa
      901 gcatttgata attacaatgc agttcctcct gcaactggaa tagttcacca agttaaccta
      961 gaatatttag caagtgttgt acatgttcgt gatgtagatg gtgaaaaaac tgcatttcca
     1021 gatacattag ttggtactga ttcacataca acaatgataa atggtattgg cgtactagga
     1081 tggggtgttg gtggtattga agctgaagct ggaatgcttg gacaaccttc ttatttccca
     1141 attccagagg ttattggtgt acgactagta aattcattac cacaaggcgc aacagcaact
     1201 gatttagcgt tgagagtaac tcaagagcta cgtaaaaaag gtgttgttgg caaatttgta
     1261 gagttctttg gtccaggtgt acaacattta ccactagcag accgtgctac aatcgcaaac
     1321 atggcacctg agtatggagc aacttgcgga ttcttcccag ttgatgatga atctcttaaa
     1381 tatatgaagt taactggtag atcagacgaa catatcgcgc ttgtaaaaga atatttgaaa
     1441 caaaaccata tgttctttga tgttgaaaaa gaagatccta attatacaga tgttatcgaa
     1501 ttggatttat caacagttga agcatcgctt tcaggaccaa aacgtcctca agatttaatt
     1561 ttcttaagtg atatgaaatc atcatttgaa aattctgtaa cagctccagc gggcaaccaa
     1621 ggacacggtt tagataaaag tgaatttgat aagaaagcag aaattaactt caaagatgga
     1681 tcaaaagcta caatgaaaac aggtgatatt gcaatagcag caattacatc atgtacaaat
     1741 acatctaacc cttatgtaat gttaggtgca ggtttagttg ctaaaaaagc agttgaaaaa
     1801 ggcttgaaag ttcctgaata cgttaaaact tctctagcac caggatcaaa agttgttacc
     1861 ggatatttaa gaggtgctgg cttacaaccc tatttagatg atttaggctt caacttggtt
     1921 ggttatggat gtacaacttg tattggtaat tcaggtcctt tattaccaga aattgaaaaa
     1981 gcgattgctg atgaggacct attagtgaca tctgtattat ctggtaaccg taactttgaa
     2041 ggtcgtatcc atcctcttgt taaagccaat tacctagctt caccacagtt agttgttgct
     2101 tatgcattag ctggatcggt tgatattgat ttacaaaatg aacctattgg taaaggtaat
     2161 gacggtgaag atgtatattt gaaagatatt tggccatcaa ttaaagaagt ttcagatacc
     2221 gttgatagtg ttgtaacacc tgaattattt attgaagaat ataataacgt atacaataac
     2281 aacgaattat ggaatgagat tgatgtaact gatcaacctc tatatgactt tgatcctaat
     2341 tcaacataca ttcaaaatcc atcattcttc caaggattat ctaaagaacc gggtacgatt
     2401 gttccgttaa atggtttacg tgttatgggt aaattcggtg attctgtgac aactgaccac
     2461 atctctccag caggtgcaat tggtaaagat acgccagctg gtaaatattt acaagatcac
     2521 caagtgccta ttcgtgaatt taattcatat ggttcaagac gtggtaatca cgaagtaatg
     2581 gttcgaggta cgtttgctaa tatacgtatt aaaaaccaat tagcgccagg tactgaaggt
     2641 ggttttacaa cttattggcc aacaaatgaa gtaatgccta tctttgatgc tgcaatgaaa
     2701 tataaagaag atggtacagg tttagttgta ttagctggta acgattatgg tatgggttca
     2761 tctcgtgact gggcagcaaa aggtacaaac ttattaggtg ttaaaacggt tattgcacaa
     2821 agttatgaac gtatccatcg ttcaaattta gttatgatgg gtgtattacc attagagttt
     2881 aaaaaaggtg aatcagctga ttctcttggt ctagatggta cagaagaaat ttctgttaat
     2941 attgatgaaa atgttcaacc acatgactac gtcaaagtta ctgctaagaa gcaagatggt
     3001 gatttggtag aatttgacgc tatggttcgt tttgactcac ttgttgaaat ggattactat
     3061 cgccacggtg gtattttaca aatggtttta agaaataaat tagcgcaata aaaaataggt
     3121 atcacagtaa attttttatc ggtatttgag gcactttgat agtgctctaa aaatagctct
     3181 ataaacttta tgatttaata ggtcggttca aaacctttgt gttcttggcc ggcctcttct
     3241 tgtattttat gttagaatga gatataaagt taaactgaaa gaaggcttaa aatgatatat
     3301 agtattacag aaatagaagc gcgttatgct gaaactgata agatgggtgt aatttatcac
     3361 gggaattatg caacttggtt tgaagttgcg cggttggatt atatatcgaa gttaggtttt
     3421 agttatgctg atatggaaaa acaaggaatc atttcacctg tgactgacct caatgtcaat
     3481 tataaaaagt ctatttttta tccagaaaaa gttaaagtta aaacttgggt tgaaaaatat
     3541 tcgagattac gttcagtgta taaatatgag atttttaatg aaaaaggtga acttgcaact
     3601 acaggttcca cagaattgat ttgcattaaa gaagatactt ttaagcctat acggttggat
     3661 cgttatttcc cagattggca tgaagcctat agtaaagtgc aagcgctcaa taatgaaggg
     3721 aaaatagtag agataatgga tggtattgat tctttataag ttactataaa tgtttggaga
     3781 ggcaatatta atatttaatc tataaaaaat agtcaatacg ttaacattaa cataattagc
     3841 gtgaagataa gctaatatat atagcaaaaa cgaacttgtt catacaacat cagatgtgct
     3901 aaaccaaagt acaacttccg aggttcagta cacatcagaa tgtgtaaaca agttcgtttt
     3961 attttagtta ttttttggaa taagaaattt catcttcgtg atcaactaca tttacaataa
     4021 tgtggtcatc ttcaaagtac cacaaatctt tttccgctac gacaacattt aaatcgtcat
     4081 attgttgttc atagccaata tcaacatctt cctttggttc aactgtaaaa gcaggactaa
     4141 atccttgctt gagttggaat tcgccaccat atcttacaaa aaacacgagc actttattat
     4201 tttcaggtaa ctcaagttca tttttaaacc aagttactgc tgcatcagta agttctattt
     4261 gcataacaat gacctcctat atttattaca gaatgggagt gggcccaaac tcgtattgtt
     4321 tgtggaattt cttttcgaaa ttctctgtgt tggagcccac cccaacctgc acattattgt
     4381 aagctgaatt tccataagct tctatgttgg gaccgccgac cagaattgaa aaaagctagt
     4441 tataagcgca tttcgttctg tcaactacag ctaatataac attgtggaac ctatgacatt
     4501 gatttatgtc tcggtctcaa atgttcttgt aaaaaaacta agtatagttg aattttaagt
     4561 taaaaaaatt gtttttgcaa aaataaaata atccatcaac tgcaaaaaaa tatgcaatga
     4621 tggactaatg tcaaaaaatt gttacatcca ttttatttta ggttcttcgc ctctaaaaat
     4681 ccttgcgata ttagagcgat gtctaattat caatatgatt gaaactaaga aactaacgac
     4741 taataaaata tagtcttgaa tgataagcga gccaatcaca cagcaaattg ctgcaacgat
     4801 acttgctaaa gaaacatatt taaaaatctt caatacaata aagaagataa ttgcaagtat
     4861 tagtaatagt atcgggttga ctcccaagac gacacctgca ctagttgcaa cagctttgcc
     4921 accttggaat tttaaataaa ctgggtaaac gtgtccaagt atagcgaata agcctacaat
     4981 taaaccattt gtaaaaaaag tactaatagg gccatctgcg tgaactggta accataaagg
     5041 gaagaaaaca gttatgaacc ctttgaaaat atctagaaat gttaccaaga atcctgcagg
     5101 acgacctaat actctaaagc tattagtagc gccagtatta ccactaccaa attgtctaat
     5161 atcttttttg aaaaataatt ttccaattac gaatccactt gggaaagcgc cgataagata
     5221 acttagtagt aacatgacga ttatcatcat aaatattaca catcctttaa tatcttagga
     5281 ctattttatc atattcgttt aaattacggc taaaattttt aaaaacgggg attaatatat
     5341 ggaattaagc tatgaaagtt aattgacact tgcattttac gctgatttat ataagaataa
     5401 ctattgtata gttttaaaaa cgaacgtacg tttgcaggag gcgaaatcat tggcaatgaa
     5461 taaacaaaat aattattcag atgattcaat acaggtttta gaggggttag aagcagttcg
     5521 taaaagacct ggtatgtata ttggatcaac tgataaacgg ggattacatc atctagtata
     5581 tgaaattgtc gataactccg tcgatgaagt attgaatggt tacggtaacg aaatagatgt
     5641 aacaattaat aaagatggta gtatttctat agaagataat ggacgtggta tgccaacagg
     5701 tatacataaa tcaggtaaac cgacagtcga agttatcttt actgttttac atgcaggagg
     5761 taaatttgga caaggcggct ataaaacttc aggtggtctt cacggcgttg gtgcttcagt
     5821 tgtaaatgca ttgagtgaat ggcttgaagt tgaaatccat cgagatggta atatatatca
     5881 tcaaagtttt aaaaacggtg gttcgccatc ttctggttta gtgaaaaaag gtaaaactaa
     5941 gaaaacaggt accaaagtaa catttaaacc tgatgacaca atttttaaag catctacatc
     6001 atttaatttt gatgttttaa g
//
LOCUS       DAMZPP010000151         5980 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMZPP010000151 DAMZPP010000000
VERSION     DAMZPP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.151"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..671)
                     /locus_tag="POH73_002407"
     CDS             complement(<1..671)
                     /locus_tag="POH73_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDI6888324.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEEL"
     gene            complement(675..1787)
                     /gene="pdhA"
                     /locus_tag="POH73_002408"
     CDS             complement(675..1787)
                     /gene="pdhA"
                     /locus_tag="POH73_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDI6888325.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQTAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            complement(1958..2584)
                     /locus_tag="POH73_002409"
     CDS             complement(1958..2584)
                     /locus_tag="POH73_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HDI6888326.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            2949..3500
                     /locus_tag="POH73_002410"
     CDS             2949..3500
                     /locus_tag="POH73_002410"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDI6888327.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
     gene            3831..3953
                     /locus_tag="POH73_002411"
     CDS             3831..3953
                     /locus_tag="POH73_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888328.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
     gene            3982..4200
                     /locus_tag="POH73_002412"
     CDS             3982..4200
                     /locus_tag="POH73_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDI6888329.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            4200..5897
                     /locus_tag="POH73_002413"
     CDS             4200..5897
                     /locus_tag="POH73_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDI6888330.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
BASE COUNT         1930 a         1053 c          923 g         2074 t
ORIGIN      
        1 agttcttctg cagctttcat tgattcttga accattgcac cgtatgtgat gattgaaatg
       61 tcattacctt cttttttcac attagcctta ccaatgtcaa ttgtatattc ttcttcaggt
      121 acttcttcac ggaatgaacg atacaatttc atatgctcta agtatacgac tgggtcatta
      181 cttctaatag aagaaattaa taaacctttc gcatcgtatg ggcctgaagg aataacaacc
      241 tttagacctg gagattgagc taaaatacct tctaagttat ctgcgtgcaa ttcaggtgtg
      301 tgtacgccac caccaaatgg gctacgaatt gttacaggtg cagttttagt accgcctgaa
      361 cggaaacgag tacgtgcaat ttgtccagca atcgcatcaa atacttcgaa tacgaaacct
      421 aagaattgta cttccataac cggacggaat ccttcaactg caagacccat tgctaaacca
      481 ccaatacctg attcagctaa aggtgtatcg aatactctat cttcaccaaa ttctttttgt
      541 agtccttcag taacacggaa aacaccgccg ttaacaccaa cgtcttcacc aaaaattaaa
      601 acatcttggt catttttaag ttcagtttta agcgcatcat taatcgcttg aaccattgtc
      661 atttgtgcca tggcttactt cgactccttc tctttgtata tttcatattg ttctgctaag
      721 ttttgaggca tatcttcata cataatttcc attagagaag taacagtttg tttttcagtg
      781 ttatcagcct ctttaattgc tgctttaata tcagcttttg cacgttcaat aacttcattt
      841 tctttgtctt cattccataa acctttgttt tcaaggaatt tacggaaacg tactaatggg
      901 tcttttttct cccattcagc atcttcatct gaagttctgt aacgagttgg atcgtcacca
      961 gccattgtat gaggaccata acgatatgtc atagtttcaa ttaatgttgg gccttcacct
     1021 gcaactgcgc ggtcacgtgc ttctttagtt gcttgatata cagctaacgc atccatacca
     1081 tcaacttgga taccaggaat acctacagca attgcttttt gagctaatgt ttcagcagca
     1141 gtttgcttgc ttcttggtgt tgaaattgca tagttattgt tttgaataac gaaaattgca
     1201 ggtgctttat aagctgctgc aaagttaata ccttcgtaga aatcaccttg tgaagaacca
     1261 ccgtcaccag tgtaagtgat tgcaactgca tttttaccac gttttttaag tgcaaatgca
     1321 acgccagcag tttgaatgta ttgtgcaccg ataataattt gtgggcttaa tgcattaacg
     1381 ccttcaggga attgatttcc tttgaagtga cctcttgaga ataagaaagc ttcagttaat
     1441 ggtaaaccat gccaaataat ttgaggaaca tctctgtatc ctggtaaaat gtaatcttct
     1501 ttttctaaag cgtattgtga cgctaattgt gatgcttctt gaccagcagt tggtgcatag
     1561 aaacctaaac gtccttgtct gtttaatgag atagaacgtt gatcaaggat acgagtccat
     1621 accattcttt ccattaattc tactaattgt tcatccgtaa gatcaggtac taagtcttca
     1681 tttacgacgt taccattctc atccaaaatt tgaaccattt caaatttcga ttgagtatca
     1741 tttaaaactt ttactgcatc gaattgggct tgtaacttag gagccattca attcaccata
     1801 cctttcccat aattgtatag aaattcattt tatccttact aattgtacca caaataaatg
     1861 ggactgttaa accatttgaa aaagttctgt tttaataaaa aaagatacag ataacaggaa
     1921 ctgtattagt tgtttcctac catctgtatc atattaatta tttaaattga tcaacgtctt
     1981 gcttttcttt accaacctta tttagtactt tcgtatactt atctgatact tcttttaact
     2041 ttttatagtt ctgttctatt gcttttgatt tttcattaac accttgttgt gtcgcatcat
     2101 tttgatttaa atatttaaat aatgtttttt ctgagtttac agccttttta tatgctttcg
     2161 cgtaatcact gtgcaactta tatttttctt ttaatacatc atctaattgt tttacttctt
     2221 tacgtttaac atcattatca atattatcaa cgtgactttt tgctttctta aagtcttgtt
     2281 cagacttctt aattgcgtct tcttcttttt caaattcctt tagacgatca tcggcatttt
     2341 taattaagtc ttttactgct ttaccgcgaa ctgctgtatc tttactattg acatcttcag
     2401 ttaatttttt ctttttctca tctaattcag caattttctt accaacagtt ttaattggtt
     2461 cttcatcgtc tttaatttta tccacttgct ttaaatatgc cttaatttct tttttatccg
     2521 tagtacatcc tgcaagcaag acactagatg ctaatactac tgcgattgtt tttccaaatt
     2581 tcattacttg tacctccact tgctatttaa attaaatgat aaataaaatc tgcttggaaa
     2641 tcaattttaa acaattacat tacacatctt cattactaag tatatctgtt gacagttacg
     2701 tcatttataa tataattttt aaagtcaaga cgataaataa atttctaaag tatcattgcc
     2761 tgaaatcaac actttaatca ttttttaggc aattagcata tctgaaaatt tacatatact
     2821 atctaaaata aaccttggca gtagttggtt aactttgtac atacttacac ataaaaatgt
     2881 atgagtatct taacaacctt tttactgtca ttcaactaaa acaaattaaa ataaggaagg
     2941 tgcaatatat gttaacaatg aaagacatca ttagagatgg tcatccaact ttgcgtcaaa
     3001 aagcagctga gctagaatta ccattaacta aagaagaaaa agaaacatta atcgccatga
     3061 gagagttttt agtaaatagt caagatgagg aaatcgcgaa acgttacggt ttacgttcag
     3121 gcgttggttt ggctgcacct caaattaata tttctaaacg tatgattgct gttttaatac
     3181 cagatgatgg cagtggcaaa tcttatgact atatgcttgt gaaccctaaa attgtaagtc
     3241 atagcgttca agaagcttat ttaccaactg gtgaaggttg ccttagtgtc gatgataatg
     3301 ttgctggtct agttcaccgt cataatagaa ttacaattaa agccaaagac atcgaaggta
     3361 atgatataca gttacgacta aaaggatatc cagcaattgt tttccaacat gaaattgatc
     3421 atttaaatgg tgtaatgttc tatgatcaca ttgacaaaga ccacccatta caaccacata
     3481 cagatgctgt agaagtttaa aacacactat tactaattat caaagcttag gataaatatg
     3541 atgtcctaag ctttttcttt tacaactttt cctaaaaaat aacagtaaaa tattttcacc
     3601 tttctaccaa actttttatc ccctcattta aattttaccg tttcatataa aatcctttaa
     3661 ttctttctta acattaattt ataatctcta catttatttt aataaataga actgcacatt
     3721 tattcgaaat acttagattt ctagtgagat aaactgcttt atttattatc attcatcatg
     3781 taaaataaga tttaactgaa attttagtgt tatttcacta attttttaaa atgaacgaca
     3841 tgatgaacct agttattaac caaatcgtta ttaagttaca ttatagagat gattggaatg
     3901 aatttatcga tatatactcc aatacgattt tactagggtt aacaataaat taaacaaaca
     3961 ttcttaggag gaatttttaa catggcagta tttaaagttt tttatcaaca taacagagac
     4021 gaggtaattg tgcgtgaaaa tacacaatca ctttatgttg aagctcaaac agaagaacaa
     4081 gtacgtcgtt acttgaaaga tcgtaatttt aatatcgaat ttatcactaa attagagggc
     4141 gcacatttag attacgaaaa agaaaactca gaacacttta atgtggagat tgctaaataa
     4201 tgaaacaatt acatccaaat gaagtaggtg tatatgcact tggaggtcta ggtgaaatcg
     4261 gtaaaaatac ttatgcagtt gagtataaag acgaaattgt cattatcgat gcaggtatca
     4321 aattccctga tgataactta ttagggattg attatgttat acctgactac acatatctag
     4381 ttcaaaacca agataaaatt gttggcctat ttataacaca tggtcacgaa gaccatatag
     4441 gcggtgtgcc cttcctatta aaacaactta atatacctat ttatggtggt cctttagcat
     4501 taggtttaat ccgtaataaa cttgaagaac atcatttatt acgtactgct aaactaaatg
     4561 aaatcaatga ggacagtgtg attaaatcta agcactttac gatttctttc tacttaacta
     4621 cgcatagtat tcctgaaact tatggcgtca tcgtagatac acctgaagga aaagtagttc
     4681 ataccggtga ctttaaattt gattttacac ctgtaggcaa accagcaaac attgctaaaa
     4741 tggctcaatt aggcgaagaa ggcgttctat gtttactttc agactcaaca aattcacttg
     4801 tgcctgattt tactttaagc gaacgtgaag ttggtcaaaa cgtagataag atcttccgca
     4861 attgtaaagg tcgtattata tttgctacct ttgcttctaa tatttaccga gttcaacaag
     4921 cagttgaagc tgctatcaaa aataaccgta aaattgttac gttcggtcgt tcgatggaaa
     4981 acaatattaa aatcggtatg gaacttggtt atattaaagc accacctgaa acatttattg
     5041 aacctaataa aattaatact gtaccgaagc atgagttatt aatactatgt actggttctc
     5101 aaggtgaacc aatggcagca ttatctagaa ttgctaatgg tactcataag caaattaaaa
     5161 ttatacctga agataccgtt gtatttagtt catcacctat cccaggtaat acaaaaagta
     5221 ttaacagaac gattaattcc ttgtataaag ctggtgcaga tgttatccac agcaagattt
     5281 ctaacatcca tacttcaggg catggttctc aaggtgatca acaattaatg cttcgattaa
     5341 tcaagccgaa atatttctta cctattcatg gtgaataccg tatgttaaaa gcacatggtg
     5401 agactggtgt tgaatgcggc gttgaagaag ataatgtctt catctttgat atcggagatg
     5461 tcttagcttt aacacacgat tcagcacgta aagctggtcg cattccatct ggtaatgtac
     5521 ttgttgatgg tagtggtatc ggtgatatcg gtaatgttgt aataagagac cgtaagctat
     5581 tatctgaaga aggtttagtt atcgttgttg ttagcattga ttttaataca aataaattac
     5641 tttctggtcc agatattatt tctcgaggat ttgtatatat gagagaatca ggacaattaa
     5701 tttatgatgc acaacgcaaa atcaaaactg atgttattag taagttaaat caaaataaag
     5761 atattcaatg gcatcagatt aaatcttcta tcattgaaac attacaacct tatttatttg
     5821 aaaaaacagc tagaaaacca atgattttac cagtcattat gaaggtaaac gaacaaaaag
     5881 aatcaaacaa taaataatca aaaagctact aactttgaag tgaagtttta attaaactca
     5941 ccacacattg ttagtagctt tttctttata tatgatgagc
//
LOCUS       DAMZPP010000152         5976 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMZPP010000152 DAMZPP010000000
VERSION     DAMZPP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.152"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(1..1494)
                     /locus_tag="POH73_002414"
     CDS             complement(1..1494)
                     /locus_tag="POH73_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6888331.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGGNDVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFETFGKTNKNGVPYMSLLVTAIIVVIIFVVQSVTEGAYQYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKWFPFGPIFAAILCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(1921..3858)
                     /gene="thrS"
                     /locus_tag="POH73_002415"
     CDS             complement(1921..3858)
                     /gene="thrS"
                     /locus_tag="POH73_002415"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDI6888332.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(4083..4178)
                     /locus_tag="POH73_002416"
     CDS             complement(4083..4178)
                     /locus_tag="POH73_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888333.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLK"
     gene            complement(4271..5191)
                     /gene="dnaI"
                     /locus_tag="POH73_002417"
     CDS             complement(4271..5191)
                     /gene="dnaI"
                     /locus_tag="POH73_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDI6888334.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(5191..>5976)
                     /locus_tag="POH73_002418"
     CDS             complement(5191..>5976)
                     /locus_tag="POH73_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDI6888335.1"
                     /translation="LGKHFISQDIVTKDAKRLITQLATLYGLTADGMKHVILNSITSG
                     QQLSFEEMRKQARSYYLMEHENQMPKLQVKSPATSSSTAKSTEANPKPQSDEWFELLE
                     QTSPIDMLASWSESEPTISQKTLVEELIEREKMSFGVINILLQFVMLKEDMKLPKAYI
                     LEIASNWKKKGIKTAKEAYNYAKKVNQPKNEGSSGNYQKRGSYYGQRNRISKEKTPKW
                     LENRDKPSEQDSAKDNSVDDQQLEQDRQAFLDKLSKKWEEDSQ"
BASE COUNT         1874 a         1205 c          833 g         2064 t
ORIGIN      
        1 ttaacctttt atttcatcca ttgacacgtc ttgacgtaaa tctacttgtt ctaatggaat
       61 tttcttcgtt ttatatcgaa gcttatgata aataaagaat gctaaaaata ctgggattcc
      121 catatacgta attaagaagc gactaaaatt aaaatctcct gttttaataa agtcaacatc
      181 ttgaccgatg attacaacaa tacataaaat agctgcaaaa ataggtccga acggaaacca
      241 tttagctgta tatttcaatt tagatttatc ataattttgt ttatcaaatg cccttctaaa
      301 tcggtaatga ctcactgcga tacctaccca ggcaataaag ccagttaacc cacttgcagc
      361 aacgatgtat tgataagcac cttctgtaac actttgtacc acaaatatga tgactacaat
      421 aattgcagtt actaataaag acatatatgg cacaccattt ttatttgttt tcccaaatgt
      481 ttcaaacgcc aatttatctt tactcattga ataaagcata cgcgttgagg catacattcc
      541 agagttacct gctgataaaa ctgatgttaa gataacagca ttcataaatg aggctgcaaa
      601 agcaaatcct gcatttttaa aaactagcgt gaacggagag gttgcaacgt cattaccgcc
      661 tcccattaat gcactactat cataaggaat taacataccg ataacaaaaa tggctaaaat
      721 gtaaaataat aaaattctcc agaatacttg tttaatcgct ttcggcacag cacgttcagg
      781 attttctgat tccccagctg taatacctat taactctgta ccttggaatg aaaaaccagc
      841 aattaagaat acacctaaaa tagataataa acttccgcct agatttccac caagaatagg
      901 accttcacct ttattaaata tttcgaatcc tacaacatga ccacccatga ttccgacaat
      961 cgttaataaa ccaattgcaa tgaaaacaat aactgtaacc acttttatca atgccaacca
     1021 gtattcactt tcaccataga cgcgaaccga taacgaattc agactaaaaa ttataactaa
     1081 gaacaacgca ctccatgccc aagctggtat gccttgtaat ggtgtccaat attgaatgac
     1141 ttgtgctgca atcgtaatat ctgctgctac agttactacc cagttaaacc aatagttcca
     1201 accaagcgca aaccctaaag atggatcaac aaatcttgta gcatatgtac taaatgagcc
     1261 tgatactggc aaataagtag ccatttcgcc aagtgacgtc attaagaaaa ataccattat
     1321 tccgataatt gcgtatccta ttaatgcacc caaagcacct gcatcatgaa ttgctccacc
     1381 agaagttaca aataaacctg taccaataca acccccaatc gcaatcatag aaatatgacg
     1441 atctttaagt ccccttttga caacattgtt actttcattt tgaacttttg acatataagc
     1501 tccctttcca tgttttagag gccaagtaat aaataacctt tatttacttt catacccttt
     1561 ttttacagaa tatagtgata ttaaaagtaa aaaagtggct acataccttt aagatatgca
     1621 accactttgg caattatcta tataaggtag cacatcacat tctgtgacag tacagttcct
     1681 attcgaaaac tgtcccaata gatataattt atggtttata tctatttcgg catctttacc
     1741 tttcacttgt tcaacttatg taccataaat acttctgaca agttactaat taaacatgca
     1801 acctctaact caatttaata ttttaactaa cttgtaatat acaggattca tcacgcataa
     1861 tcaaccctgt aaaacttgat acgcaataaa agtttcaaag cattttattg cgacaactgt
     1921 ctatctatgt tttttcaaac gaatttcatc aactagattc cagataaatt catctttttc
     1981 aactgtttct tggtcttgtg atccatattg acgcacattc acttgattat tttcaacttc
     2041 cttatcccca actacgattt gataaggtat tttttgcatt tgagcttctc taattttata
     2101 acccattttt tcattacggt catcaatact tacacgaacg ccttgagatt tcaattcatc
     2161 ttgtaattgg cgcgcataat cataatgtaa atcaacgtta actggaatga tttgaacttg
     2221 ttttggcgct aaccaagttg ggaatgcacc tttagtttct tcagttaaaa atgctacaaa
     2281 tcgttccatt gttgatacaa caccacgatg aataacaact ggacgatgat gttcaccatc
     2341 ttgaccaata taagttaaat caaaacgttc tggtaataag aaatcaagtt gtgctgttga
     2401 taatgtctct tctttaccca tcgctgtttt aacttgaaca tctagtttcg gaccatagaa
     2461 tgccgcttca ccaatcgctt cttcgtacga taagccaagc tcatccgctg cctctttaag
     2521 catattttca gctttattcc acatgtcatc atcatcaaag tacttttctt tatcttcagg
     2581 gtctctataa cttaatctaa agctataatc ctcgaaacca aagtctttat atacatcaat
     2641 aatcatgttt acaacgcgtt tgaattcttc tttaatttga tcaggtcgaa caaagatatg
     2701 tgaatcattt aaagtcatac ctcgaacacg ttgtaatcct gatacagcac cacttgcttc
     2761 atatctatgc atagttccta gctcagcgat acgtataggt aattcacgat atgagtgtgg
     2821 tttattcgca taaatcatca tatgatgtgg acagttcatt ggacgtaata ccatagattc
     2881 agtttcatct aactgcattg gtgggaacat atcttcttga tagtgatccc agtgaccaga
     2941 tgttttgtat aaatcaacat tagcaagtac gggtgtataa acgtggtcat accccatgct
     3001 aacttcttta tcaacaatgt aacgttcaat ttcacgtcta attgttgcac cgttaggtaa
     3061 ccataatggc aaaccagcac caactaattg gctatttgtg aataattcta actctttacc
     3121 aattttacga tgatcacgtt ctttacgctc ttctaacatt tgtaaatgtg cttttaattc
     3181 ttttttatca aagaaagcag taccataaat acgttgtaac attttgttgt tactatctcc
     3241 acgccagtat gcacctgctg tagataatag tttaaactct ttaattttag ctgttgatgg
     3301 aacgtgaact ccacgacata aatcagtaaa atcaccttga ctatataatg ttacattttc
     3361 atcttcagga atcgcgtcga ttaattctaa tttgtattca tcattgctga ataactcttt
     3421 cgcttcatct cgtgaaacca cttttcgttc gattttcata ttttcgttaa cgatttgttt
     3481 cattgttttt tcaatttgtt caaagtcatc agatgagatg ttttggtcaa tgtcgaagtc
     3541 atagtagaag ccaccttcta ttacaggacc tacaccaaat ttaacattac catataacct
     3601 tttaatcgcg tgtgccatta aatgtgcagt agaatgacgt aatacctcta acgcttcttc
     3661 actacctggt gtcacaattt caattgatcc atcagtttca agcggtttag ttaaatctac
     3721 aagttgcccg ttaaatttgc cggcaacagc ttttttacgt aatccaggac taattgattg
     3781 tgctatatct tcagtagtag tacctttatc aaacgccttt ttattaccat ctggaaattg
     3841 aatattaatt tgttccatgt taaaccctcc tatttgcttt ataaatactt ttatctgacc
     3901 tcgttaaaaa attgaacaac aatcatcgtt ttaacattaa acctattaac aataaacaga
     3961 gaatttatga caccaacaaa catttatgtc tgaaaaaaac aaaaaatccc gcccctatac
     4021 aagggacgag atcgtcgtgg taccacccta gttatttaat acaagttatg tattaaatat
     4081 ctctacttaa gataacggtc ttgatccggg tattcattac aaataccata aatgaagtag
     4141 taatcatcta atttattaac catattctca tcaaacaatg gctttctgtg taatattgat
     4201 tagatcatct tgtcttcaat catttaaacg atttagattt gtattcatta tacaccaatc
     4261 attttaaaat tcaattgttt ctgaaatttt ctcctgataa aaagtatggt gttgacaaag
     4321 atttgacacg ttcaataata cgtgctgctt tagtcttctc ttcaccatca cgagtcatcg
     4381 ctaaatgatg ttccaattca ctatagtcaa aattagaact aaagaatgtt ggtaattcat
     4441 gaaccattcg ataatgtagc aaaggtccaa ttacctcatc tctcacccat ggagtcactt
     4501 cttcagcccc aatatcatca agcattaaaa tgtttgcttc tcttacgcga tgtaatttct
     4561 tttcaaaaga accatcttta aagccacctt ttaatgttct aataaattcc ggtaaataaa
     4621 taattgtcga acgtaccttc ttagatttga gctgattcgc aattgcacct agaataaaag
     4681 atttacctgt cccaaatgga ccataaaggt aaaggccttt cacttgttcc ccattagtta
     4741 ttgctgtaca aatatcatct gctgccatag ctacatcaag acggtctcga tgattcatat
     4801 aaatatcttt caatttggcg tttaaagtat ctcgttgcat atgatgagat gtaattagct
     4861 cagcttcaaa gcgttcttcg tcgtacttga ttttacatgg acattgtaaa tagcgtattt
     4921 taattcggtt attatcaaca tataactcag gcacatgccc ctttacgaaa tttggacaat
     4981 cagcaaattt atgaccgtca taatgttttt gttgatcttt atactcttgt aacacattta
     5041 agtcttcatc aatcatagca ttcgttaatt cagctcgatg cgcttccaaa aattgcttaa
     5101 catctgggtc attgattact tcttttttta tcttttctat tcttttttca aagtcctgcg
     5161 acgtgttaat tatactttta aattgcttca ttattgactg tcctcctccc attttttaga
     5221 taatttatct agaaatgctt gtcgatcttg ctctaattgt tgatcatcta cgctattatc
     5281 tttagccgaa tcttgttcac taggtttatc tctattttct aaccatttag gtgttttttc
     5341 ttttgaaatg cgattacgct gcccatagta tgaaccacgc ttttggtaat ttccgctaga
     5401 accctcattt ttaggttgat taactttttt agcgtaatta tatgcttctt tagctgtctt
     5461 aatacctttt ttcttccaat ttgatgctat ttccaaaata tacgctttag gaagtttcat
     5521 atcttctttt aacatgacaa attgcaacaa aatattaatg acaccaaaag acattttttc
     5581 acgttcaatt aattcttcaa ccaatgtctt ttgcgatata gttggttctg attcagacca
     5641 agaagctaac atatcaattg gactcgtttg ttcaagtaat tcaaaccatt catcactttg
     5701 tggctttgga ttcgcttctg tagatttggc cgtcgaagat gatgtagcag gagatttcac
     5761 ctgtaattta ggcatttgat tttcgtgttc cattaagtaa tacgagcgtg cttgtttacg
     5821 catttcttca aaggataact gttgtccact tgtaattgaa tttaaaataa catgcttcat
     5881 gccatctgct gttaaaccat ataaagtcgc gagttgtgta attaaacgct ttgcatcttt
     5941 ggtaacaatg tcttgactaa taaaatgttt acctaa
//
LOCUS       DAMZPP010000153         5905 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMZPP010000153 DAMZPP010000000
VERSION     DAMZPP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.153"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            1..474
                     /locus_tag="POH73_002419"
                     /pseudo
     CDS             1..474
                     /locus_tag="POH73_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
     gene            585..1466
                     /locus_tag="POH73_002420"
     CDS             585..1466
                     /locus_tag="POH73_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6888336.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGNQ
                     RLQGYKMLVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            1953..2522
                     /locus_tag="POH73_002421"
     CDS             1953..2522
                     /locus_tag="POH73_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888337.1"
                     /translation="MALIVSRAILFLILALALGIATVFPEQKLPVYILFTLFVVVAIV
                     SNVWLTHQNVKKHIIKFDKHVAYFLGVLSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIRNTRPSNKQLKVISEQSAQQFKVISLILVSVSIVFIFIALMLKW
                     WLILAISIIVLIIITVAMIVYEKYYTTPN"
     regulatory      2575..2670
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2747..4057
                     /locus_tag="POH73_002422"
     CDS             2747..4057
                     /locus_tag="POH73_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDI6888338.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFSWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLVAILFNFPKLKKNTSQEMK"
     gene            4227..4733
                     /locus_tag="POH73_002423"
     CDS             4227..4733
                     /locus_tag="POH73_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDI6888339.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGNYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDSMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(4791..5555)
                     /locus_tag="POH73_002424"
     CDS             complement(4791..5555)
                     /locus_tag="POH73_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI6888340.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHVDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTEKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
BASE COUNT         1979 a          796 c         1189 g         1941 t
ORIGIN      
        1 gcgtttgatg aagcggtagt aacaattgga caaatatcat gtggtaatac ttggaatgtc
       61 attgctgatc atgcgtatgt acaaggcaca gtgcgtagtt ttgatcctgt ggtgcgaaag
      121 cttgtagaaa cacgattaca agatattgcg gacgggttag ctcaagcata taatatgaaa
      181 attaatttaa attatacaca tttacctggc gctgtgatga atgatgaggc attgacgcat
      241 aaagcaatag cagtagccta acatgttgga tataaggtgg aaatgatgga acagccgctt
      301 acgattggag aagatttttc aggttatagt caacattttc caagtgtatt tgcgttaatt
      361 ggttctcata gtgaatatga tttacatcac cctcaatata aaccggatga aaggattcta
      421 gaaaaagtac ctgagtattt tgttgaattt gtaaagagac tattgcatga gtaattcgag
      481 cttgttttta taaagaagaa agtaattttt agtgtaatca tgattaagtt cctaggaaga
      541 ttgggtaaat aataaccatt aagcgaataa ggagatgttt aaaaatggca gctcaagatc
      601 ctagaacaaa atttaaaacc acggattatg aaaaacaaga gcaagaggta ccgggtttac
      661 aatctgaaat gacgccagca cctgattgtg gcgaaacgtc ttaccaaggt aatcaacgct
      721 tacaaggcta taaaatgtta gttacaggtg gtgactcagc aatagggcgt gcagcagcaa
      781 ttgcgtatgc taaagaaggt gcagatgtag cgattaacta tcttccgagt gaagaacaag
      841 atgcacaaga agtacgccaa gtgattgaag aaagtggtca aaaggcagtg ttaattcctg
      901 gtgatataag agatgagcag ttcaactatg accttgttga acaagcatat caacaattag
      961 gtggcttaga taatgttact ttagttgctg gacatcaaca gtatcatgat gatattcatg
     1021 gatttacaac tgaggcattt actgaaacat ttgaaacgaa tgtctatccg ctattttgga
     1081 cagttcaaaa agctttggaa tatttaaaac cgggtgcgtc aattacgacg acatcgtctg
     1141 tacaaggtta taatcctagt cctattcttc atgattatgc cgcttcaaaa gcagcaatta
     1201 tttcattaac taaaagtttt tcagaagaat taggaccaaa aggaattaga gtgaactgtg
     1261 tagcgcctgg tccattctgg tcaccattac aaatttctgg tggacaacct caaagtaaaa
     1321 tcccaacatt tggtcaaaaa acacctttag gtcgtgcagg tcaacctgtt gagttatgtg
     1381 gaacttatgt gctattagcg tcagaagaat caagttatac aactggacaa gtgttcggtg
     1441 ttagcggtgg agtgcaaata gattaggttt gtatgaagct tttaattgaa caacataata
     1501 ttaatttagc tacttcaatc aagtaaatta aaatgaattg gtgaagtgta ttttaaatag
     1561 tcggtaaagg tgtgtatctt tatcggctta ttttaatact ttgtcataag gagcgtttgc
     1621 tcagggtgtg gattataagt tttaagcaga ataaactttc ttaatgagga tgtcaggaca
     1681 acaacgaaaa agttaaaaac tattgcggga ttgggtttag tggcggttag tggaagaaag
     1741 ttaaatttat taccaagtgt aatgcctaag gttagatatt agacgtcaga acggacaaag
     1801 aataatagga tatgttttca attaaatagc gaagtattta tatgttaaga tgatgtaaat
     1861 tacatagaaa cgctgaccgt agaagtactg atcatagaaa tgctgataaa cgtaaatttg
     1921 caaactcgat attaaacaaa ggggttgtta ttatggcatt aatagtatct agagctattt
     1981 tatttttgat tttagcatta gctttaggaa tagcgactgt ttttcctgaa caaaagttgc
     2041 cagtttatat attattcaca ctgtttgttg tggttgcgat tgtttcgaat gtatggctta
     2101 cacatcaaaa cgttaaaaaa catatcatta agtttgacaa acatgttgct tactttttgg
     2161 gtgtgctctc agggttactt tacattggat ttgtaattta tacattaact atagggagct
     2221 atgatgtttt accaattatt atatttgcaa taagtatcgt attggttcaa atattagaaa
     2281 atatactggg aatcagtata agaaatacgc gtccatcaaa caaacagttg aaagttattt
     2341 ctgaacaaag tgcacagcaa tttaaagtca tatcattaat attagtaagt gtgtctatcg
     2401 tgttcatttt tattgcatta atgcttaaat ggtggcttat acttgcgatt agcattattg
     2461 ttttgattat tattacagta gcaatgattg tttatgaaaa atattatacg actcctaatt
     2521 aatttatgat tgacgtacta aggattatct tgtaacgtaa acgtaaataa gcatcactta
     2581 tctagagagg tggagggact ggccctatga agcctcggca acatctcgaa tgtgccaatt
     2641 ccagtaaccg taatggtttg aagataagca ggtaaagtac atgaaaacct ctttcttcat
     2701 cgtttgtgag aaagaggtat ttttaattgg aaagcaggta aaaaggatgg aagtacataa
     2761 aaagagcaat gcttgggcat tattcccctt gttattattt gtggcgttgt ttttaggcgt
     2821 aggtattatc acgggtgatt ttacttcaat gccattaaat gttgcaatta cgataacggt
     2881 aattgtggcg ttattaatga atcgaaaaga atcatttgct aaaaaagttg aggttttcac
     2941 gaaaggtgca ggccattcaa acattatttt aatgatgttg atttttattt tagcaggtgc
     3001 attttcaaat acagctgaaa aaatgggtgg cgtaaagtcg acagttaatt taggactatc
     3061 attaatccca gaaaatttaa ttattgtagg attgtttgtg atttgtatgt ttgtttctat
     3121 atctatggga acgtcagtag ggactgttgc tgcaatagca cctgttggtt acggatttgc
     3181 acaagcgaca gatgtaccaa ctgcgctagc aatggctaca gttgttggag gtgcaatgtt
     3241 cggcgataat ctatcaatga tttcagatac aacaattgct gcggttagaa cacaacatac
     3301 gaaaatgaaa gataaattca gagtaaactt taaaattgta ttgccaggtg ctattttaac
     3361 gattattatt ctttatttct taacgaatgg catttcttta aatcatgcta aaaactatga
     3421 ttataattta attaaagttg taccgtatat tttagtattg gtattagcat tattaggtgt
     3481 aaatgtcatt atcgtattga ttggcggaac attattagcc ggaatcattg gtctcataga
     3541 tggttcattc agttggatgg ggctactaga tgcagtgtcc aaaggtatta taagcatgga
     3601 agatattgct atgattgcat tgctaattgg tggtttagtc ggtattattc aacacaatgg
     3661 tggtattgaa tggttgttgc aatttgttcg ctctaaagtg aaatcaaagc gtggcgcaga
     3721 attaggaatt gcgagtttgg taagtgtcgc agatattgcc acggcaaaca acaccatctc
     3781 tattattatg tcaggtcctt tagctaaaaa tattgcagat gaatatgatg tagattcaag
     3841 gaaatcagca agtattcttg atatatttgg aggctgcttc caaggtttat taccatatag
     3901 cccgcaagtc atttcagcgg ctggtgtagc tggtatttca ccgttaatgt tattcccata
     3961 tagcatttat cctatattat taggtgtatg cggacttgtt gcgattttat ttaattttcc
     4021 aaagctaaaa aagaacacta gtcaagaaat gaaatagtaa tgcttaacaa tatatcgatg
     4081 tagccaagta aggtggcttg ttatattaac agatcatgtt gtactcaccg tataacgcta
     4141 atcttttaaa aatgatgatc atctatgttt ggaactgagc aaaaatacgt taaaataaaa
     4201 gtaagagcga aagtgggtgg acaacagtgg caaaatttaa tgtagagaat gaacatgtcg
     4261 aagttgaaat tgaaaagctt tataaatttt ctccagagtt agtatacgaa gcttggacaa
     4321 agaaagattt gttaaaacaa tggtttatga catcagccag aacgaataaa gagattgaag
     4381 cggatgttaa agaaggcggt aattatcgta ttgtagatca acaacgtaat ggcaaagtaa
     4441 atgtgattga aggtatttat gaatctttag ttatggatga atacgttaag atgactatcg
     4501 gtatgcctgg actaagtgaa acacaagatg tcattgaggt agaatttttt gaacgtgaaa
     4561 caggtggcac ccaaatgcta ttttattatc gttcattagt tgaaaaggaa agacgattta
     4621 ctaatttaga atacaagcaa aagaaaaaag aatatcatga ttccatggtg catggtttcg
     4681 aattgatgtt tgataaaatg taccacgtta tcgaaacatc aacacaacaa taaaatgata
     4741 aaaggaagtt atgataggct caaggccatt aaatcataac ttcctttttt ctaatttgaa
     4801 atatcattat gtagtaaata ctcattatat tttcttacaa gtgtttcaaa gaatatatga
     4861 taactgatga agctagaata gtctgtacca tcagttaagt agaatgtgtt actggataca
     4921 tagacgttgt aatttgcttt ttgagcaagg gtattatctt tcattgtagt aacagaaata
     4981 aagtattttt gtctaagttg aagcaagttt gttacttcta ataattcatg tgtttcacct
     5041 gataatgaaa taataaagaa taaatcatta ggttcactct tgttcaaaat cattttaagt
     5101 tcatgttcat catgaagtac aatgacatga ttatgtaacg ttaataatat acgctgtgcc
     5161 tctgaagcaa cgtttaactg tgcacgccct gtaccataca aatatattgt tgaagctgta
     5221 tgcatttttt cagtaagaag ctggtaatca acgcgttcta aataactgaa tgtactatcg
     5281 atttcttgct tgaaactatc gatagagtta gatggtaatt ggtgtgtctt attactttca
     5341 aatttaaggt acgatttaaa atcactataa ccatcaaaac ctaatttacg tgtgaatcga
     5401 tgaatggttg cattcgaggc atgtgtgaat tgcgctaaat cttgtatctt catattttta
     5461 cattcatcaa cgtgtgtgtt gatgaagtgg gcgatatgta aatcattatc atttagtttg
     5521 tcaaagtttc tattaatgtg ttcgtctaaa aacatattca acctccttgg aaaataaatt
     5581 tgtgatatat ggtttttaca ttaaaaggtg aaattttaga aattaatttt ttgattgtat
     5641 ataaggttat acatggctat agtacttaca tattttaaaa tcgagcatat tctcgacatg
     5701 cttgtgaaaa aattttcact atatgaaaag ataatctgat tatacaaaat actttcaaaa
     5761 tacgtcaatg tcgttgtagg tgtgtaagcg tttactatat attatattta aatagtaagg
     5821 catctattta taaatgaatc taattagatg tattgttagg aaagatgata tggagggaat
     5881 agaagatgaa tgcgataaag cgttt
//
LOCUS       DAMZPP010000154         5898 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMZPP010000154 DAMZPP010000000
VERSION     DAMZPP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5898
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.154"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(245..1105)
                     /gene="folD"
                     /locus_tag="POH73_002425"
     CDS             complement(245..1105)
                     /gene="folD"
                     /locus_tag="POH73_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDI6888341.1"
                     /translation="MVAKILDGKQIAKDYRQGLQNQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPIPK
                     QVSEQKILEAINPDKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            1306..1788
                     /gene="purE"
                     /locus_tag="POH73_002426"
     CDS             1306..1788
                     /gene="purE"
                     /locus_tag="POH73_002426"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDI6888342.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKTM
                     VQFASEARERGIDIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            1775..2899
                     /gene="purK"
                     /locus_tag="POH73_002427"
     CDS             1775..2899
                     /gene="purK"
                     /locus_tag="POH73_002427"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDI6888343.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVAVLDPAEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESKDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGIKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITYFPLQENEHRNQI
                     LFKTIVPARIDKIAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            2903..3607
                     /locus_tag="POH73_002428"
     CDS             2903..3607
                     /locus_tag="POH73_002428"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDI6888344.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHIKLLNIATDKDIETLKTKALEINQVLK
                     QLMHAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            3607..3870
                     /gene="purS"
                     /locus_tag="POH73_002429"
     CDS             3607..3870
                     /gene="purS"
                     /locus_tag="POH73_002429"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDI6888345.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLEDEKENA"
     gene            3872..4543
                     /gene="purQ"
                     /locus_tag="POH73_002430"
     CDS             3872..4543
                     /gene="purQ"
                     /locus_tag="POH73_002430"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HDI6888346.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPVISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQKLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            4536..>5898
                     /gene="purL"
                     /locus_tag="POH73_002431"
     CDS             4536..>5898
                     /gene="purL"
                     /locus_tag="POH73_002431"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDI6888347.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGEHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGIGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYEDEVYADIPVEPLADEAPVYILEGEEKNYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIA"
BASE COUNT         2077 a          872 c         1105 g         1844 t
ORIGIN      
        1 ttgttacaag cttaatttca tttaattaac tattgtctat ataaaattgt tgagcctaga
       61 acaatgtgaa atgctccctt caaagccttc atttattcaa tgtctacttt gaagggagca
      121 tttgatcgat taatgtccca gatttttctt gttattcatt gcaattgcaa aataatattt
      181 aatttgggtt aaaagagttc attaaaactc atttattaat tcacattaca taacttatgt
      241 gaatttacga atcaatacct cgacgcattt tttctgcaag caaagtatta tttaatacca
      301 tagtaattgt taatggacca acgccaccag gaactggtgt aatagctcca gcaatttctt
      361 taaccgcatc ataatcaacg tcacctttta atttgccatt ttcatctggc gtattgccaa
      421 catctataat tactgctcct tctttgacaa catcttttgt tactaaacca ggcttaccaa
      481 ctgcactgac aatgacatca gcatctttta aatatgatgc catatccttt gaacgagaat
      541 gtaagattgt tactgatgca tttttttgaa gtagtaactt agaaactggt tgtccgacaa
      601 tatgacttcg tccgattaca actgcatttt taccttctaa atcaatatca gcatgtttta
      661 atatttccat gatgccgagc ggtgtgcaag gtacaaaagt ttgttcgtcg atatataatt
      721 tccctatatt tattggatgg aaaccgtcca catctttatc aggattgatt gcttctaata
      781 ttttctgttc gctaacttgt tttggtattg gtacttgtac caaaatacca cttacagaat
      841 catcattatt tagtctgttt agttcgttta atacttcttc ttcagtagct gtttcttcca
      901 aatgtacgat ttctgaaatc ataccaattt tttcagctgc tttctttttt gatctaacat
      961 aactttgact agcgccatca ttaccaacta atataacgga taatttaggt gtaaaaccct
     1021 tttcttttag cgcttcaact tgattttgta acccctgtct gtagtctttg gcaatttgtt
     1081 taccatctaa aattttagca accataaaaa cttctcctcc tattgtcaat atcctaactt
     1141 tgttagttta taaaactaat ttacatagca aaagacgtaa attcaaacaa aaaatcaaaa
     1201 taaagttcga tttttgattg aaaaagcaga aattgcttgt tatgctatat ctataatata
     1261 caactaaagt aattcacaat tcaaactttt gaaagggtgt acaaagtgaa agtagcagtc
     1321 attatgggca gttcttccga ttggaaaatt atgcaagaga gttgtaacat gttggattat
     1381 tttgaaattc cgtacgaaaa acaagtagta tccgcacatc gtacgccaaa aacgatggtt
     1441 caatttgctt ctgaagcgag agaaagaggt atagacatta tcattgcagg cgctggcggt
     1501 gcggcacatt taccaggtat ggttgcatca ttaacgacgc taccagttat tggagtgccg
     1561 attgaaacaa aaagtttaaa gggtatagat tctttattat caattgttca aatgccagga
     1621 ggtattccgg ttgcaacgac tgcaattggt gcagcaggtg ctaaaaatgc aggtatactt
     1681 gcagcaagaa tgttaagtat tcaaaatcct tctttagttg aaaaactaaa tcagtatgaa
     1741 tcttcgttaa ttcaaaaagt ggaggacatg caaaatgaac ttcaataaat taaagtttgg
     1801 tgcgactatt ggcattattg gtggtggtca gcttggaaag atgatggcac aatcagctca
     1861 aaaaatgggt tataaagtgg ctgtattgga tcctgctgaa gattgtccat gtagatacgt
     1921 tgcacacgaa tttatacaag ccaagtatga cgatgaaaag gcactcaatc aattaggaca
     1981 aaaatgtgat gtgattactt atgaatttga aaacatttca gcccaacaat taaaactatt
     2041 atgtgaaaag tacaatattc cgcaaggtta ccaagctata cagttattac aagaccgctt
     2101 aactgaaaaa gaaacattaa aaagtgctgg taccaaggtt gtcccattca tttcagtaaa
     2161 agaatctaaa gatattgaca aagcaattga aacattagga tatcctttta ttgtaaaaac
     2221 tagatttggt ggctacgatg gcaaaggtca agttttaatt aacaacgaaa aagacttaca
     2281 agaaggtatt aaattaattg aaactagtga atgcgtagct gaaaaatatt tgaatatcaa
     2341 gaaagaagta tcacttactg ttacaagagg aaacaacaat caaatcactt atttcccatt
     2401 acaagaaaat gagcatagaa atcaaatact tttcaaaaca attgttccag cgagaataga
     2461 taaaatagct gaggcgaaag agcaagttaa taaaattatc caatcgattc atttcattgg
     2521 aacatttaca gttgaatttt ttatagatag taataaccaa ttgtatgtga acgagattgc
     2581 accaaggcct cacaattccg gacattattc aattgaggca tgtgattatt cacaatttga
     2641 tactcatatt ttagcagtta ccggacaatc attaccaaat tcaattgaat tattaaagcc
     2701 tgcagtcatg atgaacttac taggtaaaga cttagattta ttggaaaatg aatttaatga
     2761 acatccagag tggcacttac atatttatgg taagtctgag cgtaaagata gcagaaaaat
     2821 ggggcatatg actgtactaa cgaatgatgt aaaccaaact gaacaagata tgtacgctaa
     2881 atttgagggg agtaattaaa gcatgacatt actatatgaa ggaaaagcga agcgcatttt
     2941 ctcaacaaat caagaaaatg aattaagagt tgaatataaa gatgaagtta ctgctggaaa
     3001 cggggctaag aaagacacaa tggcaggtaa ggggcgatta aataatcaaa ttacttctat
     3061 tatatttaaa tatttacaag aaaatggaat agaaagtcac tttattaagc aattatctga
     3121 aacagaacaa ttagttaagc ctgtgaaaat aattccatta gaagtagttg ttcgtaatat
     3181 tgctagcgga tctattacaa agcgtttagg ttttgaaaat ggtgaagttt ttagagaacc
     3241 acttgtggaa tttttctata aaaatgatgc attaaatgat cctttgatta cagatgatca
     3301 catcaaatta ctgaatattg cgacagataa agatattgaa acattaaaaa cgaaagcgct
     3361 agaaattaat caagtattaa aacaattaat gcatgcgatg aatttaaaat tagtagattt
     3421 taaaatcgaa tttggaaaga ctgagactgg tcaaattttg ttagcggatg aaatatctcc
     3481 agatacatgt cgaatttggg ataaagctac caatgcaaac tttgataaag atgtatatag
     3541 aaataacact ggatcactga ttgaaacata tcaaatattt ttaaacaaat tggaggattt
     3601 aaaataatga aaacaattga actacatatc acattacaac cacaagtatt agatacgcaa
     3661 ggacaaacgc ttactcgagc tgtacatgat ttaggttatg cacaagtgaa tgatattcgt
     3721 gtaggaaaag tattatatat gacagtggat gaggttagtg atgaaaaggt acacaacatt
     3781 attacaactc taagtgaaaa attgtttgca aatacagtga ttgaagaata tagctataaa
     3841 gttttagaag atgaaaagga gaatgcataa aatgaaattt gcggttcttg tttttccagg
     3901 ttcgaattgt gatagagaca tgtttaatgc tgctattaaa agtggtgttg aagcggaata
     3961 tgtagattat agagaaacat cactaagtgg atttgatgga gtacttattc ctggtggatt
     4021 ttcattcggg gattacttaa gatctggggc aatggctagt gtagcgccgg ttatttctga
     4081 agttaaacgt cttgcagctg aaggtaagcc agtattaggt gtttgtaatg ggtttcaaat
     4141 tttaactgaa ataggcttat tacctggtgc attattgcat aacgattcac atttatttat
     4201 tagtagaaat gaagagttag aaatagtgaa taatcaaacg gcttttacaa atctttatga
     4261 acaaggtgaa aaagttatat atcctgtagc tcacggcgaa ggtcattatt attgtactga
     4321 tgaaatatat caaaaattaa aagctaacaa tcaaattatt ctgaaatatg tgaataatcc
     4381 gaacggttca tatgacgata ttgcaggaat tgttaacgaa aaaggcaatg tgtgtggcat
     4441 gatgccacat cctgaaagag ctttagaaac gttgttaggt actgatagtg gtgtgaaatt
     4501 atttgaagcg atggtaaaaa gttggaggga acaacatgtc taaatttatc gaaccaagcg
     4561 ttgaagaaat taaacttgaa aaagtatatc aagatatggg attaagtgat caagaatatg
     4621 aaaaagtttg cgatatttta ggcagacaac ctaactttac agaaacaggt atcttttctg
     4681 ttatgtggag tgaacattgc tcttataaac attctaaacc gtttttaaaa caatttccta
     4741 cgtcaggtga gcatgtgctt atgggtccag gtgaaggtgc aggggtagtc gatataggtg
     4801 ataatcaagc cgtagtattt aaagtagaat ctcacaatca tccatcagca attgagccat
     4861 atcaaggtgc tgctacaggc gttggtggaa tcattcgtga cattgtctct attggggcta
     4921 gacctattaa tttgttaaac agtcttagat ttggagaatt agataataaa caaaaccaaa
     4981 gattacttaa aggtgttgta aagggtatcg gaggttatgg taactgcatt ggtattccaa
     5041 caactgctgg agaaatcgaa tttgatgaac gttatgatgg taatccactt gttaatgcaa
     5101 tgtgtgttgg tgttatcaat cacgacatga ttcaaaaagg cacagcaaaa ggtataggta
     5161 attcggtcat ttatgttggt ttgaaaactg gtcgagatgg tattcatggt gctacttttg
     5221 catctgaaga attgacggaa gaaagcgaaa gtaaacgacc ttctgtacaa atcggtgatc
     5281 catttgtagg taaaaaatta atggaagcaa cacttgaagc aattacattt gatgaattag
     5341 ttggtattca agatatgggt gctgctggtt taacatcttc atcttctgaa atggcggcaa
     5401 aaggtggtag tggtttacat ttgagattag aacaagtgcc aacacgtgag ccaggtattt
     5461 ctccttacga aatgatgctt tcagaaactc aagaacgtat gttactagtt gttgaaaaag
     5521 gtactgaaca aaaattctta gatttatttg ataagcacga attggatagt gctgttatag
     5581 gtgaagtaac agatacaaat cgttttgttt taacatatga agacgaagtt tatgctgaca
     5641 ttccagttga accactagct gatgaagcac ctgtatatat tttagaagga gaagaaaaaa
     5701 attataatac ttctaaaaat gattatacac acatcgatgt taaagatact ttctttaaat
     5761 tacttaagca tccgactata gcatctaaac actatttata tgatcaatac gatcaacaag
     5821 ttggtgccaa tacgataatt aagccaggac ttcaagcatc ggtagtacgt gtggaaggca
     5881 caaataaggc aattgctt
//
LOCUS       DAMZPP010000155         5683 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMZPP010000155 DAMZPP010000000
VERSION     DAMZPP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.155"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..135
                     /locus_tag="POH73_002432"
     CDS             <1..135
                     /locus_tag="POH73_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDI6888348.1"
                     /translation="HHQYIEFYDYPKMVHDFPIYPIRQSHKAIKQIAKSIDEDVTQNN
                     "
     gene            complement(243..662)
                     /locus_tag="POH73_002433"
     CDS             complement(243..662)
                     /locus_tag="POH73_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888349.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(659..1150)
                     /locus_tag="POH73_002434"
     CDS             complement(659..1150)
                     /locus_tag="POH73_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDI6888350.1"
                     /translation="MVIITQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQPTITWPEQLGHKIAIPHKG
                     ALK"
     gene            1247..2194
                     /gene="corA"
                     /locus_tag="POH73_002435"
     CDS             1247..2194
                     /gene="corA"
                     /locus_tag="POH73_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDI6888351.1"
                     /translation="MALTIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFSFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            2225..3274
                     /locus_tag="POH73_002436"
     CDS             2225..3274
                     /locus_tag="POH73_002436"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDI6888352.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMTYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRSLNIHRG"
     gene            complement(3322..4530)
                     /gene="gltS"
                     /locus_tag="POH73_002437"
     CDS             complement(3322..4530)
                     /gene="gltS"
                     /locus_tag="POH73_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDI6888353.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(4866..5474)
                     /locus_tag="POH73_002438"
     CDS             complement(4866..5474)
                     /locus_tag="POH73_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDI6888354.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEDG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
BASE COUNT         1992 a          879 c          973 g         1839 t
ORIGIN      
        1 catcatcaat acattgagtt ttatgattat cctaagatgg tacatgattt tccaatttat
       61 ccaattagac aatcacataa agctattaaa caaattgcca aatcgataga tgaggatgta
      121 acacaaaaca actaataaca tataaatgac ccagttacga tttcatggta gaagatattt
      181 aacatgaaaa cgcaactggg ttttgttttt gaaaatatag caaggattat atagactaat
      241 tgttaatgag cttctgagtg aaaatcacca ataagtgtat tgatatgtgt gatgataaag
      301 tattgccctt taatttgata aaattgatct aaaaaattat cgaattgacg tggaatatca
      361 ctaagttctg caaagttagc tgttttatca taattgcgat gagcaacctc attttgaaat
      421 ttatcataat gttctaggaa atgttgatct tcaagctgtt gttccacaat agtcataata
      481 ttatcgatag cttgcttttg tccttgaaca taagccatat gcacgccacg cttaagtaac
      541 tgatttgctg gtccgttaat gtataaccct ttttgtgcat ttaattctgt taattcaaac
      601 ttaacatcat ctaataaatc tagtagttcg atatgaatct tttgttgtaa tgcatctgtc
      661 attttaatgc tcctttgtgt ggaattgcta ttttatgtcc taattgttca ggccaagtga
      721 tagttggttg atttttataa tagtgtgcta tttgtgttga aaatgattca ggaaaagcat
      781 cagaacgacg tgtgtgctga ttaattccca tcatcattac ttcatttgtt gatgctagtg
      841 tcccatcttc tttagttaat gttaaaaata aatgcaaccg cttataatcg taatcataaa
      901 tatataaagt aacagtaaat acatcgccaa gagacaattc tgagaggtat gtcgtatgtt
      961 cttctagtgt aaatagcgta tatgctaaat tttctcgttc ttttaaagaa agaccgtggc
     1021 tgtaattaaa acgattcacg acgtcactaa aaatgatatt ataatttgca tcatgcatat
     1081 gattgttatg gtcgatgaat tcagatgtta cggtttgagt atgtgtaaaa agttgtgtga
     1141 taataaccat ctccttagtg actatcatac taaaataaat taagacatac acgttattta
     1201 attcatgata aaataataga gaattcgtta tttaggggga gtatcgatgg ctttaacgat
     1261 acggtatcaa actacatacg aaccgtttaa agtagttgat gatatcaaag agataccaaa
     1321 agatgcgact attgtttggt atgattttga tgaaccgaat gaacaagaaa atgaatggtt
     1381 taaagcacat tttagtttta atgatttaga agtggatgat gcaattaacg gtatgccacg
     1441 agctaaatat aaatcttata aagattatca atacttagta ttccatagta ttatggggtc
     1501 aaactattca ccaatagcac taaatatttt tatacaagac aatgtattag taacatatca
     1561 tcatcaaacg ttggaatcat taaataaagt agtctataag tatatgaata cattagatgc
     1621 agaattagat tgtgcagatg tcgtcatatt aattttagat atgatggttg ataaatattt
     1681 caactttgta tatgcacttg aagatagtgt ttatcatttt gaagaccgcc atgtagatga
     1741 tcgctttaat aaaatggtca tggatagtgt ctttaagtta cgttcggatt tgattaaagt
     1801 aaaacgtgta ctgtttccta tgcaggagct aattgataca atgaaacaaa atggtgattt
     1861 gattatagat aataaacatt cgctatacat tcaacatatc gatgaccact tgattaagca
     1921 acgtaatatc attcgtactg cacaagaaat gacaaatgaa atacgagaaa attttgagtc
     1981 atacacgtca tttagaatga atagtattat gcaagtatta acattagtat cagttatatt
     2041 ttcaccgtta acattcatag ctgggattta tggtatgaac tttgtaaata tgccggcatt
     2101 acatttacat tatggatatt atatttgctt agctgtaatg tttgttatag ctgtcgtatt
     2161 aatcatcttt ttcagacgta aaaaatggtt ttaaattaga aatgacgaaa aggtaggttt
     2221 aatcatgagt gattttcaaa gagaacagag aaaaaatgaa catgttgaaa tagcaatggc
     2281 ccaatctgac gcaatgcatt cagattttga taagatgcgt tttgtgcatc attctatccc
     2341 atcaattaat gtaaatgata tcgatttgac atcacagaca cctgacttaa cgatgacata
     2401 tccggtttat attaatgcaa tgacgggtgg tagcgagtgg acgaaaaaca tcaatgaaaa
     2461 gctagctgta gttgcaagag aaactggctt agcgatggca gttggatcaa cacatgcagc
     2521 attgagaaat ccacgcatgg ctgagacatt tacgattgcg cgaaaaatga atcctgaagg
     2581 catgattttt agcaatgttg gtgcggacgt accagtagaa aaggctttgg aagcagttga
     2641 attacttgag gcacaagcgt tacaaatcca tgttaattct cctcaagaat tagttatgcc
     2701 tgaagggaat cgtgaatttg tgacttggtt agataatata gcgtcgattg tatcacgagt
     2761 atctgttcca gtcattataa aagaagttgg atttggtatg agcaaagaat taatgcatga
     2821 cttacaacaa ataggcgtta agtatgtcga tgttagtggc aaaggtggta ctaactttgt
     2881 agatattgaa aatgaacgtc gtgcaaataa agatatggat tacttatcat catggggaca
     2941 gtctacagtt gagtcattac ttgaaacaac ggcttatcaa agcgaaattt cagttttcgc
     3001 gagtggtggt ttacgtacac cactcgatgc aattaaaagt ttagcacttg gtgcaaaggc
     3061 aactggtatg tcacgtccgt ttttaaatca agttgaaaat aatggcattg cacatacagt
     3121 agcttatgta gaatcattta ttgaacacat gaaatcaata atgacgatgt tagatgctaa
     3181 aaatattgac gacttaacac aaaaacaaat cgtatttagt ccggaaatat tgtcatggat
     3241 agaacaacgt agcttgaata tacatcgagg ataaataaaa gaggaggcga aggcctcctc
     3301 ttttatttat gagtttggtg tttaactaaa ccattgaatg aatcccataa tgactataac
     3361 accaattaaa tcgattaaga atgcaccaac aataggtaca actaaatatg ctttaggtga
     3421 gtttccatat tttttagtaa taacatctaa atttgccatg gcatttggcg ttgcaccaag
     3481 cccatgcccg ataaaaccac ctaccattac tgcagcatca taatcttttc ctaaacctct
     3541 aaataaaatt aaaacagcaa ataaaatcat aacgacaact tgaactaaaa cgataataat
     3601 aagaggtata gcaagtttat aaatttcgat taattgaatg ctcattagcg caagagataa
     3661 gaaaatacct aatgcgacat cgccaatttg attagtaatt tttaaatcta caatattaaa
     3721 attaaaactt tcagagatat ttcggacaat aaccgctaca aataatgagc caacgtaaat
     3781 tggaacatta atccctgttt gagctgtaaa taaatgactg aaataacttc caactgccat
     3841 acagaataca acgattgtaa attgaatgaa gaatacttca gtggcattaa atttactatg
     3901 taaatgttcg ttatatgcaa cttggctata atctttaaat gtatcgtcac tatgttgagg
     3961 ctttaagtta taacgtttga ttaaaaattt tacaactggt ccgccgataa gccctccaaa
     4021 tacaagacct aaagttgcag ccgcaagtgc tgctgtcaat gctgagtcaa tgcctaaatc
     4081 ttggattgtc ttaccataag cggcagcatt accgtgacca ccttccatag acatagaacc
     4141 agctgttaat cctagtaacg gtttaatatt taatactttt gctagtgata caccaactat
     4201 gttttgaatg actgaaatga tagcacaaaa cataaagtat aatagcaaga ctttgccacc
     4261 taatttaaat aatttcaatg atgcaccaag accgattgtc gtaaagaatg ctaacatgaa
     4321 gaaatcttga atgaatgaag catctaattt aatcttgacc atgccaaatg aatccaaagc
     4381 cgcaactaaa atagcaaaga ttaagccacc aatcactggt gctggtatac aaatgcgttt
     4441 taaaaaatta acgtgattaa cgatagcttt accaagtaag taaagtatac aggctaaaca
     4501 taatgttgta attgcattaa gttctatcat tttcaaatcc cccttgttga atatccctct
     4561 ccctaaataa gcttttatat gtaagcgtta taaaagggca cttttaacat tatacatgca
     4621 ataaatcaat atttctagtt tttgaaaata atatcaataa tattaataca tctaaattta
     4681 ataattataa ataattctat tgttatattt taaaattaag aacaagttca aaaagaggag
     4741 atgaatcaaa aaatatgatg gacacaaaga aaaacaatca ccccatccgc atgttgacgg
     4801 ctgattgatt gacttataag gagatagatt tcattttcat gatagtaatc aatgaaagat
     4861 gacatttatt tccaagtatc ttcgggaaac atacaatctg atttacgcat gcgagacaca
     4921 aatggattac ctttcactgt gtaacgtaaa gatttatgtg tccaatcacc tttatttgga
     4981 ataccgattc gtggactagc aataatatct ttaggatatt tacgattctt agtatcaata
     5041 gacaatctac agtcatttaa cgtagcgcca tcgatagccc gtggaatgtt aaatgcctta
     5101 gtccattttc ctggaccatt cgttacctcg tagcctttct tacctctgtt acggaacata
     5161 gcggataaac cgtcttctgg ttcaattgcg cggataagta cgccttcagg tataccttca
     5221 gattttgtta caaaattaat gagtaaatgc gtatgcatga catgtgcata aattgtacca
     5281 ccacgtttat ataatgacgt aactttaggt gttattttac caccatagcc atgagccgca
     5341 cgatcattca aacctaagta tgcttccgtt tccacgatgt agcctgtata cgtttgagtg
     5401 gtatcctgat aaatcacttt gacacctaat aagtttttag caatttgtct tgtatcatta
     5461 ttaacgaaat ccataatatt ctcctcgcaa ttatatgatt aatttaaata aaaatgaaaa
     5521 agatattttt attgtagtaa atgtttgata aaatagtatt gttaagaatt ataaaaatgt
     5581 aaagtatata ctttgttatg gaatatttta tttgcagaga ttgagactct tttaaacgtg
     5641 aacattgcag tatgacatat ttttagtata gttaacgaag cgt
//
LOCUS       DAMZPP010000156         5671 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMZPP010000156 DAMZPP010000000
VERSION     DAMZPP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.157"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            64..792
                     /gene="treR"
                     /locus_tag="POH73_002439"
     CDS             64..792
                     /gene="treR"
                     /locus_tag="POH73_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDI6888355.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRTK"
     gene            854..1123
                     /gene="ffs"
                     /locus_tag="POH73_002440"
     ncRNA           854..1123
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="POH73_002440"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            1193..1294
                     /locus_tag="POH73_002441"
     CDS             1193..1294
                     /locus_tag="POH73_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888356.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYMKFDSSGMDAQFE"
     gene            1433..1957
                     /locus_tag="POH73_002442"
     CDS             1433..1957
                     /locus_tag="POH73_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6888357.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEMNSDDKTYYGLAIASLSVNPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            2026..3723
                     /gene="dnaX"
                     /locus_tag="POH73_002443"
     CDS             2026..3723
                     /gene="dnaX"
                     /locus_tag="POH73_002443"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDI6888358.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPTQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            3813..4130
                     /locus_tag="POH73_002444"
     CDS             3813..4130
                     /locus_tag="POH73_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDI6888359.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            4137..4733
                     /gene="recR"
                     /locus_tag="POH73_002445"
     CDS             4137..4733
                     /gene="recR"
                     /locus_tag="POH73_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDI6888360.1"
                     /translation="MHYPEPISKLINSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            5075..5150
                     /locus_tag="POH73_002446"
                     /pseudo
     CDS             5075..5150
                     /locus_tag="POH73_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1955 a          746 c         1215 g         1755 t
ORIGIN      
        1 agcgggtttg gtgcaattgt aatcgaaaca aacgcgtaaa ataaattgag tggatgcgtt
       61 tatatggcga aacaaaaaaa gtttatgaag atttatgagg cgttgaaaga agatatatta
      121 aacgggcaga ttcaatatgg tgaacaaatt ccgtctgaac atgatttggt gcaattgtac
      181 cagtcatctc gagagaccgt gcgtaaggca ttagatttgt tggcattaga cggcatgatt
      241 caaaagattc atggtaaagg gtcacttgtc atttatcaag aggttacaga gtttccattt
      301 tctgaacttg ttagttttaa agaaatgcaa gaagaaatgg gtgtcgcata tttaactgaa
      361 gttgttgtga atgaggttgt tgaagcgcat gaagtgccag aagttcaaca tgctttaaac
      421 atcaattcta gtgaatcact cattcatatt gttagaactc gtcggcttaa ccaacatgtg
      481 aagattgttg atgaagatta ttttctaaag tcgattgttt cagacatagg taatgatgtt
      541 gcgagtgatt ctatttatga ttatttggaa aaggtattaa atcttaatat tagttattca
      601 agtaagtcta ttacttttga accgtttgat gaacaagcat atcaattgtt tggtgatgta
      661 tcagtggctt attcagcaac agttcgaagt attgtgtatt tagaaaatac aatgccgttt
      721 caatataata tttcaaaaca tcttgcaaat gaatttaaat ttaatgactt ctcaagacgt
      781 cgtacaaagt aaacaatgat ataaatgatt tatacttgca attaactatt aaaatatagt
      841 aatatatatc ttgccgtgct aggtggggag gtagcggttc cctgtactcg aaatccgctt
      901 tatgcgaggc ttaaatcctt tgttgaggcc gtatttttgc gaagtctgcc caaagcacgt
      961 agtgtttgaa gatttcggtc ctatgcaata tgaacccatg aaccatgtca ggtcctgacg
     1021 gaagcagcat taagtggatc atcatatgtg ccgtagggta gccgagattt agctaacgac
     1081 tttggttacg ttcgtgaatt acgttcgatg cttaggtgca cggtttttta tttttaaaaa
     1141 tattaaaccg attattaaga gttgaaaata tataattata gaagctactt tcttgaagac
     1201 aattcagcgt attatacgtg gaacatgttt gtgggaagta gcttttttat atatgaagtt
     1261 tgattcaagt ggaatggatg cgcagtttga atgatttttt acggatataa aaagtaagaa
     1321 gttattattt gatgataaag aaatgatggt gaaatgaggg ggagtacctt acaatagaat
     1381 tattaatgag atacgttatg attattgaca atcaaatgcc tacggaggac atatgcaaat
     1441 atatttaagt actttaacag agttagatta tgataaatct ttaaatagta ttgaagaaag
     1501 ttttgatgat aatcctgaaa cgagttggca agcacgtgcg aaagtaaaac atttaagaaa
     1561 atctccttgc tataattttg aattagaagt aattgcgaaa aatgaaaata acgatgtcgt
     1621 tggacacgtt ttattaattg aagtagaaat gaatagtgat gataagacgt attatggttt
     1681 ggcgattgct tctttatcag tcaaccctga attgcgtgga caaaaattag gtcgtggctt
     1741 ggttcaagca gtagaagagc gtgcaaaagc gcaagagtat agtacggttg ttgtagacca
     1801 ttgttttgac tactttgaaa agttgggtta tcaaaatgct gctgagcatg acattaaatt
     1861 agaatctggt gatgcaccgt tacttgtaaa atatttatgg gataatttga cggatgcacc
     1921 acacggaatc gtaaaatttc cagaacattt ttattaattg ttcatttaag aagtaaaggt
     1981 attatcatgc tataatgaaa ggtaattgtt tatggaggtg ctaacttgaa ttatcaagcc
     2041 ttatatcgta tgtacagacc ccaaagtttc gaggatgtcg tcggacaaga acatgtcacg
     2101 aagacattgc gcaatgcgat ttcgaaagaa aaacagtcgc atgcatatat ttttagtggt
     2161 ccgagaggta cggggaaaac gagtattgcc aaagtgtttg ctaaagcaat caactgttta
     2221 aatagcactg atggagaacc ttgtaatgaa tgtcatattt gtaaaggcat tacgcagggg
     2281 actaattcag atgtgataga aattgatgct gctagtaata atggcgttga tgaaataaga
     2341 aatattagag acaaagttaa atatgcacca agtgaatcga aatataaagt ttatattata
     2401 gatgaggtgc acatgctaac aacaggtgct tttaatgccc ttttaaagac gttagaagaa
     2461 cctccagcac acgctatttt tatattggca acgacagaac cacataaaat ccctccaaca
     2521 atcatttcta gggcacaacg ttttgatttt aaagcaatta gcctagatca aattgttgaa
     2581 cgtttaaaat ttgtagcaga tgcacaacaa attgaatgtg aagatgaagc cttggcattt
     2641 atcgctaaag cgtctgaagg gggtatgcgt gatgcattaa gtattatgga tcaggctatt
     2701 gctttcggcg atggcacatt gacattacaa gatgccctaa atgttacggg tagcgttcat
     2761 gatgaagcgt tggatcactt gtttgatgat attgtacaag gtgacgtaca agcatctttt
     2821 aaaaaatacc atcagtttat aacagaaggt aaagaagtga atcgcctaat aaatgatatg
     2881 atttattttg tcagagatac gattatgaat aaaacatctg agaaagatac tgagtatcga
     2941 gcactgatga acttagaatt agatatgtta tatcaaatga ttgatcttat taatgataca
     3001 ttagtgtcga ttcgttttag tgtgaatcaa aacgttcatt ttgaagtatt gttagtaaaa
     3061 ttagctgagc agattaaggg tcaaccacaa gtgattgcga atgtagctga accagcacaa
     3121 attgcttcat cgccaaacac agatgtattg ttgcaacgta tggaacagtt agagcaagaa
     3181 ctaaaaacac taaaagcaca aggagtgagt gttgctccta ctcaaaaatc ttcgaaaaag
     3241 cctgcgagag gtatacaaaa atctaaaaat gcattttcaa tgcaacaaat tgcaaaagtg
     3301 ctagataaag cgaataaggc agatatcaaa ttgttgaaag atcattggca agaagtgatt
     3361 gaccatgcca aaaacaatga taaaaaatca ctcgttagtt tattgcaaaa ttcggaacct
     3421 gtggcggcaa gtgaagatca cgtacttgtg aaatttgagg aagagatcca ttgtgaaatc
     3481 gtcaataaag acgacgagaa acgtagtagt atagaaagtg ttgtatgtaa tatcgttaat
     3541 aaaaacgtta aagttgttgg tgtaccatca gatcaatggc aaagagttcg aacggagtat
     3601 ttacaaaatc gtaaaaacga aggcgatgat atgccaaagc aacaagcaca acaaacagat
     3661 attgctcaaa aagcaaaaga tcttttcggt gaagaaactg tacatgtgat agatgaagag
     3721 tgatacatga caagcgatat aatcgtatgt ataatgaaag aaacatcatt ttattgataa
     3781 atatttattg attttcaagg aggaaatgga atatgcgcgg tggcggaaac atgcaacaaa
     3841 tgatgaaaca aatgcaaaaa atgcaaaaga aaatggctca agaacaagaa aaacttaaag
     3901 aagagcgtat tgtaggaaca gctggcggtg gcatggttgc agttactgta actggtcata
     3961 aagaagttgt cgacgttgaa atcaaagaag aagctgtaga cccagatgat attgaaatgc
     4021 tacaagactt agtgttagca gctactaatg aagcgatgaa taaagctgat gagcttactc
     4081 aagaacgttt aggtaaacat actcaaggct taaacatccc tggaatgtga tcatagatgc
     4141 attatccaga acctatatca aagcttatta atagctttat gaaattgcca ggcattggtc
     4201 caaagacagc ccaacgtctg gcttttcata ccttagatat gaaagaagac gatgttgttc
     4261 agtttgccaa agcattagta gatgttaaaa gagaattaac atattgtagc gtatgtggtc
     4321 acattactga aaatgatcca tgttatattt gtgaagataa gcaaagagat cgttcagtta
     4381 tttgtgttgt ggaagatgac aaagatgtca tagctatgga aaaaatgaga gaatacaaag
     4441 gtttatatca cgttttacat gggtctattt cgcctatgga tggaattgga ccagaagata
     4501 ttaatattcc ttcattgatt gaacgcttga aaaacgatga agttagcgaa ttaatcttag
     4561 ctatgaaccc gaacttagag ggggaatcta cagccatgta tatttctaga ttagttaagc
     4621 ctataggtat caaagtgacg agattagcac aagggttatc tgtaggtggc gatttagagt
     4681 atgctgacga agtaacatta tctaaagcaa tcgcaggtag aacagaaatg taatgtcttc
     4741 tattaaacat tttagaattt aatactatag taagaaaagt cacagtgtaa tcattgtggc
     4801 tttttttatg gtgtggtgta ctactttatt tgtggtgtga cggtggcatg gtttacctag
     4861 ttttactgag gggtggggaa tctttaggaa gcaaaccatt ggttgtgatt tgttatttct
     4921 aatagtaatg aagtgaattg gattatcgaa gtagatctat ggttatggtg tgttggtggc
     4981 atggtttacc tggttttact gagggatggg taatcttaaa gaagcaaatc gttggttgtg
     5041 atttgttact tctaatagta atgattcgaa ttgaatcatc gaagtaggaa tactgttgtg
     5101 gtgtgttggt gctattaatt tgataaatgc ggttgatggt taatgactag gcaaatgaaa
     5161 ttcttttgta attgaaatga tagatgctgg cttagtagtt gtacttcttt ggtctaaagc
     5221 ttattaaatc agcctgtaaa gcggtgtttt gagagattat ttaaaacttg taaatttatt
     5281 tttaatttct ggtaaaaaaa taaagttctg ttttgcggtt ttttcgattg atatatttag
     5341 agaaaaacct gtttcttgtt ctaaaaaacg tactatctat aagtgggggt tttttaagtt
     5401 cgatttttag aatgagagcg ttcagaatgg atagtaaagg tgtaattttt actgttgtta
     5461 agcagtttga aagcctgtat agtatttatt tgttgaggca aacaaaacaa ctcaacttaa
     5521 gaaataactt gaattactaa cgaaaattaa ttttaaaaag ttgttgactt aaatgttaat
     5581 aaaatgtata attaattctt gtcggtaaga aaaatgaaca ttgaaaactg aatgacaata
     5641 tgtcaacgtt aattccaaaa aacgtaacga t
//
LOCUS       DAMZPP010000157         5629 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMZPP010000157 DAMZPP010000000
VERSION     DAMZPP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.158"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..333
                     /gene="nrdI"
                     /locus_tag="POH73_002447"
     CDS             <1..333
                     /gene="nrdI"
                     /locus_tag="POH73_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDI6888361.1"
                     /translation="NTLEITAENCMEPVHEPFIIVTGTIGFGEVPEPVQSFLEVNHQY
                     IRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFELHGKNKDVIEFKNKVGNFNENH
                     GREKVQSY"
     gene            296..2401
                     /gene="nrdE"
                     /locus_tag="POH73_002448"
     CDS             296..2401
                     /gene="nrdE"
                     /locus_tag="POH73_002448"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDI6888362.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            2521..3492
                     /gene="nrdF"
                     /locus_tag="POH73_002449"
     CDS             2521..3492
                     /gene="nrdF"
                     /locus_tag="POH73_002449"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDI6888363.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            3869..4840
                     /locus_tag="POH73_002450"
     CDS             3869..4840
                     /locus_tag="POH73_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6888364.1"
                     /translation="MKYLLKGKILLLLLILLTIISLFIGVSELSIKDIFHLSDSQQNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSISGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            4827..>5629
                     /locus_tag="POH73_002451"
     CDS             4827..>5629
                     /locus_tag="POH73_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDI6888365.1"
                     /translation="MRNNNKKIMLLIAVTFLISMLYLFVGIDFDIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVIILVITIFLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYIL"
BASE COUNT         1967 a          785 c          978 g         1899 t
ORIGIN      
        1 aatacgcttg agattacagc agaaaattgt atggaaccag ttcatgaacc gtttattatc
       61 gttactggca ctattggatt tggagaagta ccagaacccg ttcaatcttt tttagaagtt
      121 aatcatcaat acatcagagg tgtggcagct agcggtaatc gaaattgggg actaaatttc
      181 gcaaaagcgg gtcgcacgat atcagaagag tataatgtcc ctttattaat gaagtttgag
      241 ttacatggaa aaaacaaaga cgttattgaa tttaagaaca aggtgggtaa ttttaatgaa
      301 aaccatggaa gagaaaaagt acaatcatat tgaattaaat aatgaggtca ctaaacgaag
      361 agaagatgga ttctttagtt tagaaaaaga ccaagaagct ttagtagctt atttagaaga
      421 agtaaaagat aaaacaatct tcttcgacac tgaaatcgag cgtttacgtt atttagtaga
      481 caacgatttt tatttcaatg tgtttgatat ttatagtgaa gcggatctaa ttgaaatcac
      541 tgattatgca aaatcaatcc cgtttaattt tgcaagttat atgtcagcta gtaagttttt
      601 caaagattac gctttgaaaa caaatgataa aagtcaatat ttagaagact ataatcaaca
      661 cgttgcaatt gttgctttat acctagcaaa tggtaataaa gcacaagcta aacaatttat
      721 ttctgctatg gttgaacaaa gatatcaacc agcgacacca acatttttaa acgccggccg
      781 tgcgcgtcgt ggtgagctag tgtcatgttt cttattagaa gtggatgaca gcttaaattc
      841 aattaacttt attgattcaa ctgcaaaaca attaagtaaa attggtggcg gcgtagcaat
      901 taacttatct aaattgcgtg cacgtggtga agcgattaaa ggaattaaag gcgtagcgaa
      961 aggtgtttta cctattgcta agtcacttga aggtggcttt agctatgcag atcaactagg
     1021 tcaacgccct ggtgctggtg ctgtgtactt aaatatcttc cattatgatg tagaagaatt
     1081 tttagatact aaaaaagtaa atgcggatga agatttacgt ttatctacaa tatcaactgg
     1141 tttaattgtt ccatctaaat tcttcgattt agctaaagaa ggtaaagact tttatatgtt
     1201 tgcacctcat acagttaaag aagaatacgg tgttacatta gacgatattg atttagaaaa
     1261 atattatgat gatatggttg caaatccaaa tgttgagaaa aagaaaaaga atgcacgtga
     1321 aatgttgaat ttaattgcgc aaacacaatt acaatcaggc tatccatatt taatgtttaa
     1381 agataatgct aatagagttc atccgaattc aaatattgga caaattaaga tgagtaactt
     1441 atgtactgaa attttccaat tacaagaaac ttcaattatt aacgactatg gtattgaaga
     1501 cgaaattaaa cgtgatattt cttgtaactt aggttcatta aacattgtaa atgtaatgga
     1561 aagcggaaaa ttcagagatt cagttcactc tggtatggac gcattaactg ttgtaagtga
     1621 tgtagcaaat attcaaaatg ctccaggagt tagaaaagct aatagtgaat tacattcagt
     1681 tggtcttggt gtgatgaatt tacatggtta cttagcaaaa aataaaattg gttatgagtc
     1741 agaagaagca aaagattttg caaatatctt ctttatgatg atgaatttct actcaatcga
     1801 acgttcaatg gaaatcgcta aagaacgtgg tatcaaatat caagactttg aaaagtctga
     1861 ttatgctaat ggcaaatatt tcgagttcta tacaactcaa gaatttgaac ctcaattcga
     1921 aaaagtacgt gaattatttg acggtatggc tattcctact tctgaggatt ggaagaaact
     1981 acaacaagat gttgaacaat atggtttata tcatgcatat agattagcta ttgctccaac
     2041 acaaagtatt tcttatgttc aaaatgctac aagttctgta atgccaatag tagatcaaat
     2101 tgaacgtcgt acttatggta atgcggaaac attttaccct atgccattct tatcgcctca
     2161 aacaatgtgg tactacaaat ctgcattcaa tactgatcag atgaaattaa tcgatttaat
     2221 tgcgacaatt caaacgcata ttgaccaagg tatctcaacg atactttatg ttaattctga
     2281 aatttctaca cgtgagttag caagattata tgtatatgcg cactataaag gattaaaatc
     2341 actttactat actagaaata aattattaag tgtagaagaa tgtacaagtt gttctatcta
     2401 ataagtaaac gtaaaaaatg acaaacaact aatcatctcg tttaaaataa cagatgatta
     2461 gtctgccatg tctatatttg tcaattattg agagtaacat tacaggagga aattatattc
     2521 atgatagctg ttaattggaa cacacaagaa gatatgacga atatgttttg gagacaaaat
     2581 atatctcaaa tgtgggttga aacagaattt aaagtatcaa aagacattgc aagttggaag
     2641 actttgtcag aagctgaaca agacacattt aaaaaagcat tagctggttt aacaggctta
     2701 gatacacatc aagcagatga tggtatgcct ttagtcatgc tacatacgac tgacttaaga
     2761 aaaaaagcag tttattcatt tatggcgatg atggagcaaa tacacgcgaa aagttattcg
     2821 catattttca caacgctatt accatctagt gaaacaaact atttattaga tgaatgggtt
     2881 ttagaagagc cacatttaaa atataaatca gataaaattg ttgctaatta ccacaaactt
     2941 tggggtaaag aagcttccat atatgaccaa tatatggcca gagttacgag tgtattttta
     3001 gaaacattct tattcttctc tggtttctat tatccgctat atttagctgg tcaaggaaaa
     3061 atgacgacat caggcgaaat cattcgtaaa attcttttag atgaatctat tcatggtgtg
     3121 tttactggtt tagatgcgca gcatttacga aatgaactat ctgaaagcga gaagcaaaaa
     3181 gctgaccaag aaatgtataa attgctaaat gacttgtatt taaatgaaga gtcatacaca
     3241 aaaatgttat acgatgatct tggaatcact gaagatgtgt taaactatgt taaatataat
     3301 ggaaataaag cactttcaaa tttaggtttt gaaccttatt ttgaggaacg tgaatttaac
     3361 ccaattattg agaatgcctt agatacaaca actaaaaacc atgacttctt ctctgtaaaa
     3421 ggtgatggtt atgtattagc attaaatgta gaagcattac aagatgatga ctttgtattt
     3481 gacaacaaat aataattaaa attaaaaaga ccttcacatg aaagggtaat agcgattcgt
     3541 ttcgtcttga ctcctacatg ttgaaggtct tttttctata acttaaaaac gtatgcacat
     3601 tttgaaaaag taaaaatggc atttaatgat gccaatgagg ctagaaagta tacaagatac
     3661 tgcattacaa aagggtgtgc tattttacta tatacatcta aattacacat ttacaaaaaa
     3721 tgatttctat tttaattgca aatatcaata aaattgacaa taaattattt gaaaggctat
     3781 tgaaatgagt gacaaaaaac gataccatta atgagaaatg ttatcaatga taataattat
     3841 cattaaatta aagggagaaa attttgtaat gaagtattta ttaaagggaa aaattttgct
     3901 tctattactt atattgctaa caattatttc gttgtttata ggtgtcagtg aactgtctat
     3961 taaagatatc tttcatttgt cagactcaca gcaaaatata ttgttctcaa gccgaatacc
     4021 gagaacgatg agtattttaa ttgctggaag ttcattagct ttagctggcc taatcatgca
     4081 acaaatgatg caaaataagt ttgttagtcc aactacagct gggacgatgg agtgggcaaa
     4141 attaggaatc ttaattgccc tattattctt tccaactggt catattttat taaaactagt
     4201 atttgctgtt atttgcagca tcagcggaac atttttattt gttaaaatta ttgattttat
     4261 caaagtgaaa gatgtcatct ttgtaccact attagggatt atgatgggtg gcattgtcgc
     4321 tagttttaca acgtttatct cattacgtac aaacgcagtt caaagcattg gtaactggct
     4381 taacgggaac tttgccatta tcacaagtgg acgttatgaa atcttgtatt taagtattcc
     4441 tcttttagca ttgacatatc tttttgcaaa tcatttcaca attgtaggta tgggtaaaga
     4501 ctttaccaat aatttaggtt tgagttatga aaaattaatc aatattgctc tatttattac
     4561 tgccacaatt acggcattag tcgttgtgac agttggaact ttacccttct taggtttagt
     4621 tattccaaat attatttcga tttatagagg cgatcattta aaaaatgcaa ttcctcatac
     4681 gatgatgtta ggtgccatct ttgtattgtt ttctgatata gttggcagaa ttgtagttta
     4741 tccatatgaa attaatattg gtttaacaat aggtgtattt ggaacaatca ttttccttat
     4801 cttacttatg aaaggtagga aaaattatgc gcaacaataa taaaaaaata atgcttttaa
     4861 ttgcagtcac gttcttaatt agtatgctgt acttatttgt tggtattgat tttgatatat
     4921 ttgaatatca attttcaagt cgtttaagaa agttcatatt aattatttta gtaggtgctg
     4981 ccattgcaac atccgttgtt attttccaag cgattacaaa taatcgttta ttgacaccat
     5041 caataatggg attagatgca gtttatttat ttatcaaagt attgcccgtc tttttatttg
     5101 gaattcaatc ggtatgggtt actaatgtat atttgaactt tatattaaca cttataacga
     5161 tggtgttatt cgcactaatc ctattccaag gtatctttaa aattggacat ttttcaattt
     5221 actttatctt acttataggt gtccttttag gaacattttt tagaagtatt acaggtttta
     5281 ttcaattgat tatggatcct gagtcatttt tagcaataca aagtagtatg tttgctaatt
     5341 ttaatgcttc taattcgaat ttagttactt tttcagcagt attattagta atcatattag
     5401 tcattacaat ttttctattg ccatatttag atgtattgct tttaggtcgt gctgaagcaa
     5461 ttaatctggg gatatcgtat gaaaaattaa cgcggattct acttgtaata gtttctgtct
     5521 tagtctctgt gtcaactgca ttagtaggac caattacatt tttaggctta ttgactgtaa
     5581 atctagcgca tgaactaatg aagacgtatg aacataaata tatattaat
//
LOCUS       DAMZPP010000158         5555 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMZPP010000158 DAMZPP010000000
VERSION     DAMZPP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.159"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..562)
                     /locus_tag="POH73_002452"
     CDS             complement(<1..562)
                     /locus_tag="POH73_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6888366.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHAVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPLATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNWPD"
     gene            879..2477
                     /locus_tag="POH73_002453"
     CDS             879..2477
                     /locus_tag="POH73_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDI6888367.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTLTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTVSEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            2858..4336
                     /gene="mqo"
                     /locus_tag="POH73_002454"
     CDS             2858..4336
                     /gene="mqo"
                     /locus_tag="POH73_002454"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDI6888368.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKNVLFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVEIQYKQNVLDIKKQKSGA
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(4463..5545)
                     /locus_tag="POH73_002455"
     CDS             complement(4463..5545)
                     /locus_tag="POH73_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000696075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6888369.1"
                     /translation="MKILAIVRRVLKEMLRDKRTLALMFLAPILILTLMYFIFNGDEN
                     DLKIGIDTSVSSNITKQLPSSDIKTQTFHSHENIKSKIENNHLDGYIYQENQTLYVIY
                     TNEDPNKSNMIKQMIGKAIQQDKISSIKHLIEKAPPTMKEPNKQDIKISHSYLYGNSD
                     STYFDKMFPILMGFFVFLFVFLISGIALLRERTTGTLERVLSTSIRRSEIVLGYLIGY
                     GIFAIIQTLIIVLFSIYLLNINLAGSLWYVILINICLAITALSMGIFISTFANSEFQM
                     IQFIPLVAVPQVFFSGIFPLENMPDWLGNIGYIFPLRYAGDALTNVMIKGQGWSNIWF
                     DLLILMIFIIVFIILNIVGLKRYRQV"
BASE COUNT         1897 a          807 c          983 g         1868 t
ORIGIN      
        1 tatctggcca attaagttgt tctgaatcct cgataaaaag gttgcgaaaa ggtataggat
       61 gatgatcgat ataagccatt ttattcaaat aaaactgaga catcatatca aaatcttcaa
      121 tagttttaat agtttcccat attgaagcac ttgttgctaa aggggcttta aaatcaaaaa
      181 cgacatgacc atcgattgat aaatcacctt tcagaagtgt caataaagct aaatcgaaaa
      241 tcgtttctac agagtaataa tgtattaaat caagagtctg ttgaatcgca tatatcaggt
      301 taggataata attattgttt tgagcccaat cggcaatgtt gatcgatgat tgaccgaaat
      361 agtgacaatc atttttgtat tgtccatcca cagtataatc aaccacgata gacttaggat
      421 ataattgaac cgcatctaac atagattgat aaatcattat ctcattggca tatggaacgg
      481 catgtgtata accttcagac tgagctttca ttaattgaga ttcaaacttt tgcatattag
      541 aaacatttat ctgtttaatc attgctataa cttccatttc tcatcgtagt atcttcctta
      601 ttttagcacg gtcttatgta aaattcatga atagtataag ttacttcatt acattaagta
      661 caattcattt tgatgaacag atttaaaatg aaaaataaaa caattaaata ggaaagtgat
      721 attgttaata gtctgaaaaa atggtatatt ttaattgtga aaaaaatcac attaatctca
      781 ctgttctgta aaatgttatc gataacaaaa tgctatagcc agagcatgat tttaatgaag
      841 caattacact actactttat ggaaagaaga gtcagcctat gttagtaaat acgtttaacc
      901 catttgataa tttattatta tcgtctttaa ttgcagcaat accgattgta ctgtttttac
      961 tttgtttaac tgtttttaaa atgaaaggta tttatgcagc cattacaaca cttgttgtaa
     1021 cattactaat tgcaatacca tttttcaaat tgccagttgg tatcgcttct ggtgcagtag
     1081 tcgaaggttt cttccaaggc atcattccga ttggctatat cgttatgatg gcggtattgt
     1141 tatataaaat tactgttgaa tctggacaat ttttaacaat tcaagatagt attacaaata
     1201 tttcacaaga ccaacgtatt caagttttac ttattggatt tgcattcaac gcatttttag
     1261 aaggtgcagc aggatttggt gtaccaattg caatttgtgc acttttatta acacaattag
     1321 gatttaatcc gttaaaagct gcgatgttat gtttagtcgc aaatgcagcg tctggtgctt
     1381 ttggtgcgat tggtatccct gtaggtgttg tagaaacgtt gaaattacct ggagatgttt
     1441 cagtattagg tgtttctcaa tcagcaactt taacattggc aatcataaat ttcattattc
     1501 cattcttact tatctttatt attgatggtt ttagaggtgt taaagaaaca ttaccagcaa
     1561 ttttagtagt ttcaatcact tatacactta cgcaaggatt attaactgtt ttcagcggac
     1621 ctgaattagc agatattatt ccgccgttat taacaatgtt agcattagca gtattttcta
     1681 aaaaattcca accaaaacac atttatcgtg ttaataaaga tgaagaaatt gaacctgcaa
     1741 aagcacattc tgcaaaagca gtattacatg catggagtcc attcattgta ttaacagtca
     1801 ttgtaatgat ttggagtgcg ccattcttta aaaacttatt cttaccaaat ggtgctttat
     1861 catcattagt atttaaattc aacttacctg gaacagtcag cgaagttacg cataaaccat
     1921 tagtattgac tttaaatatt attggacaaa caggtacagc tattttatta actattatta
     1981 ttacaatttt aatgtctaaa aaggttaact ttaaagatgc aggtagatta ttcggtgtta
     2041 catttaaaga attatggtta ccagttctta caatttgttt catcttagca atttctaaaa
     2101 tcacaactta tggtggttta agtgcggcaa tgggtcaagg tattgctaaa gcaggtaatg
     2161 tcttcccagt tctatcacca atcttaggtt ggataggtgt gtttatgaca ggatcagttg
     2221 taaataacaa ctcattattt gcaccaattc aagcttctgt tgcacaacaa attggaacaa
     2281 gtggttcact tcttgtatct gctaatacag ttggtggtgt agcggcaaaa ttgatttcac
     2341 cacaatctat tgcaattgca actgcagcag taaaacaagt tggtaaggaa tcagaattat
     2401 taaaaatgac attgaaatac agtgtatgtt tactaatatt catctgtatt tggactttca
     2461 tcttgtcatt attataaata aacgtatttc aaaatataaa tatacagagg gtgagatgtt
     2521 ttctaacatc tcatcttttt ttaatggatc attgatgaaa gaagtttgac attataataa
     2581 tggtagcgct ttatgttaaa atgaagagtg agtaatcagc aatcaaatta aattggttga
     2641 taggtgttaa ggtttgtggt tttgtctttg tgctatcgca cataaagtat ataattaaag
     2701 tagtttcgtt attataaaat attaatatac atagtagata gtaatagagc atcaccatga
     2761 gaacctgttg agacacttat tgattcaaag tggtattaat atgtcgtatt tctcgaacgt
     2821 tccattattc attttaaaaa gggggactgt atttgttatg acaacacaac atagcaaaac
     2881 agatgtcatc ttaattggtg gcggtattat gagtgcaaca ttaggaacat tacttaaaga
     2941 attatcacct gagaaaaata ttaaagtgtt tgaaaaatta gcacaacctg gcgaagagag
     3001 ttcaaatgta tggaataatg ccggtacagg gcattcagca ctttgcgagt tgaactatac
     3061 aaaagaaggt aaggatggca cagttgattg tagtaaagca attaagataa atgagcagta
     3121 ccaaatttca aagcagtttt gggcatattt agttaaaaca ggacaattag ataacccaga
     3181 tcgctttatt caagcggtgc cacacatgag ttttgtcatt ggcgaagata acgtagcttt
     3241 tataaaaagt cgtgttgcaa cgttaaagaa aaatgtttta ttcgaaaaaa tgaaattatc
     3301 gcaagatgaa gaagaaatga aatcttgggt accgttaatg attgaagggc gtaagtctga
     3361 tgaaccaatt gctttaactt atgatgaaac tggtacagat gttaactttg gtgcgttaac
     3421 tgcaaagtta tttgataatt tagaacaacg tggtgtggaa attcaatata agcaaaatgt
     3481 attagacatc aagaaacaga aatctggagc atggctagtt aaagttaaag atttagaaac
     3541 taacgaaacg acaacatatg aatctgattt tgtatttatt ggtgctggcg gtgcgagttt
     3601 accattactc caaaagactg gaattaaaca atcaaaacat attggtggtt tcccggtaag
     3661 tggattattc ctgcgctgta caaatcaaga agtgattgat cgtcatcatg ctaaagtgta
     3721 cggaaaagca gcagtgggtg cgccaccaat gtcagtgcca cacttagata cacgttttgt
     3781 agacggcaag cgttcattgt tatttggtcc atttgcaggt ttctcaccta aatttttaaa
     3841 aacaggttca catatggatt taattaaatc ggttaaacca aataatatcg tgacgatgtt
     3901 atctgcaggt atcaaagaaa tgagtcttac gaagtattta gtgtcacaat tgatgttatc
     3961 taatgatgag cgtatggatg atttaagagt ctttttccca aatgctaaaa atgaagattg
     4021 ggaagtgatt acagcagggc aacgtgtcca agtaatcaag gatacggagg attctaaagg
     4081 taacttacaa tttggtaccg aagtcattac gtcagatgat ggcacattag ctgcattact
     4141 tggtgcatca cctggtgcgt caacagctgt agatattatg tttgatgttt tacagcgatg
     4201 ctatcgtgat gaattcaaag gatgggaacc aaagattaaa gaaatggtgc cgtcatttgg
     4261 ttatcgctta acagatcatg aggatttata tcacaaaatt aatgaagaag taactaagta
     4321 tttacaagtt aaataataaa cgaaacggta atgtcttttt aaatgtgata gacattaccg
     4381 ttttttagtg gttcttttta ttatcagtgc tttatattta gaagcttatc ttgattgaag
     4441 tcatttaaaa gttttggaga atttatacct gtctgtatcg ttttaaacca acaatattta
     4501 agataataaa gactatgata aagatcatta gaattagtaa atcgaaccat atattactcc
     4561 aaccttgccc cttaatcata acattggtta aagcatcacc agcatatctg agagggaaga
     4621 tatagccaat gtttcctagc caatcaggca tattttcaag tggaaaaatt cctgaaaaga
     4681 atacctgagg tacagcaact agtggaataa attgaatcat ttgaaactca gaattagcaa
     4741 acgttgaaat gaaaattccc atagataatg ctgtaatagc taaacaaata ttaatcaaaa
     4801 ttacatacca taagctacct gctaaattaa tgttcaataa atatattgaa aataggacta
     4861 tgattaatgt ttgaatgatt gcgaatatac catagccaat cagataacca agaacgattt
     4921 cacttcgacg aattgaagtt gataatacac gttctaacgt acctgttgta cgttcacgta
     4981 ataaagctat acctgatatt agaaatacaa ataaaaatac aaagaagccc attagtattg
     5041 gaaacatttt atcgaaataa gtactatcag aattaccgta taaataggaa tgtgaaatct
     5101 taatatcttg tttgtttggt tctttcatgg tcggaggtgc tttttcaatt agatgcttta
     5161 tactgcttat tttatcttgt tgtatagctt tgccaatcat ttgtttgatc atattagatt
     5221 tgttagggtc ttcatttgta taaatgacat ataatgtttg attttcttgg taaatataac
     5281 catcaagatg attattctcg attttagatt tgatattttc atgagaatgg aacgtttgag
     5341 tttttatgtc ggacgatggc aattgttttg ttatatttga tgaaacagat gtatcgatac
     5401 cgatttttaa atcgttttca tcaccgttaa aaataaaata cataagcgtc aatattaaaa
     5461 ttggtgctag aaacattagc gcgagtgtac gtttatcacg taacatttct tttaatactc
     5521 gtctaacaat agctaaaatt ttcatgattg acaaa
//
LOCUS       DAMZPP010000159         5424 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMZPP010000159 DAMZPP010000000
VERSION     DAMZPP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.160"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(307..537)
                     /locus_tag="POH73_002456"
     CDS             complement(307..537)
                     /locus_tag="POH73_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888370.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            complement(568..1506)
                     /locus_tag="POH73_002457"
     CDS             complement(568..1506)
                     /locus_tag="POH73_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HDI6888371.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAFE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNGRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            complement(1942..2607)
                     /locus_tag="POH73_002458"
     CDS             complement(1942..2607)
                     /locus_tag="POH73_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6888372.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFEGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFEN
                     ETD"
     gene            2759..3079
                     /gene="czrA"
                     /locus_tag="POH73_002459"
     CDS             2759..3079
                     /gene="czrA"
                     /locus_tag="POH73_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HDI6888373.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            3081..4061
                     /gene="czrB"
                     /locus_tag="POH73_002460"
     CDS             3081..4061
                     /gene="czrB"
                     /locus_tag="POH73_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HDI6888374.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALIAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVIATIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHSHHAHHHAHVH"
     gene            4327..5418
                     /locus_tag="POH73_002461"
     CDS             4327..5418
                     /locus_tag="POH73_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6888375.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     VQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLVGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLVIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVITI
                     ILFVVIHAVILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
BASE COUNT         1834 a          956 c          748 g         1886 t
ORIGIN      
        1 ataaaaaaca tgcttgcata cactatgcaa taaaaattac aaaattatat atgataccac
       61 tatgcttgct tatctctata gcgccattga tacgcatttt taaatatcta tacgccttta
      121 gaattttagc atgtcttaat tttcattaaa tattaattac ttcattttat atacaccaac
      181 aaaaaccccc tcactactat gcaagtgaga ggttatgttg atgtgcttta ttttcaacaa
      241 tgtcattaac acaatcatat ttctgctgtt aatgcactgt actatattca aacgcgctat
      301 cgactattaa tttgaagcta caaaggtacc gcataatggc agcacattaa ttaacaatgc
      361 gctaaacttt aatgaggcgc tgatttgctt gtctttaaaa gtcaacatag cactgataat
      421 tcctaaaata gccactatac gtggaattgt catgctcgga tactgcggta tatgtaagaa
      481 agcaccaaga cctgctatca caccacatac taacgcaata tatagaaatt gcttcaaatc
      541 attcactcct aaattgttat tacactatta cacatagcta atcatcaatg tgaaatcacc
      601 ttcaaagaca ctatccaaat cttcagaagt caaaataaag tttgtaccag tagtcagttt
      661 gaaaatttca ccatcgacaa tcatttgccc ttcaccttcc aacactgtaa ctaaacagaa
      721 ctctctaggc ttcatataat ttaacgtgcc agaaatttcc catttaacca atgtaaagaa
      781 atcattcgat acaatgtgtg tacacttatg gttctcaata atttcacttt caggcaaaat
      841 attaggtaat ggtgcattgt actgaataac gtctaaagct ttttcaatat ttaacggtct
      901 accattatat tgattatctt gacgattgaa atcataaagt ctatatgtaa tgtctgacga
      961 ttgcatcgtt tcgtatgcta gtattcctga agaaatagta tgtactgttc ctgctggaat
     1021 aaaatagaat tctccaggtt ttactttaat atatctaagt atcgactcta tcgttccgtg
     1081 ttgaacatga ttcgcaactt cttctctaga ctctgctaat gtccctataa ctatttctgc
     1141 atcttcttca gcatctataa tataccaaca ttcagatttg ccatattgcc cattttcatg
     1201 ttcaaaagca taagaattat cagggtgcac atgaatagaa agtgattctc ttgcatccac
     1261 tattttagtt agaagcggaa aatctttgct tgggaaatca ccaaacaatt cacgatgttc
     1321 tgaccaaata cggtctaatg tttgaccttg atatggtcca ttaataatct cgctcgtacc
     1381 atttggatgt gctgacacac accaacattc cccagttgta tcattgtcta attgatatcc
     1441 aaactcactt agacgttgac cgccccataa ttttgttttt aaaattggtt gtaaaaataa
     1501 tggcattgtt gcacctccat tgtgattaag taagcaatag aactctgatg ttgttgttcc
     1561 attatatttt gattttgttc tcatttatat cgtattataa acttccacat ttcaaattaa
     1621 ctattagtga ttgtaccata tttactaaca ttgcagtact gccaattaaa gcgcttcact
     1681 taaatttaca gtactttaac atttttaaaa ttttatagca tagagattat atctctctta
     1741 catttgtaca tatttctctt taaatttact cgcccattat accaattaat aaacaacttt
     1801 aatagttgtg ccatacattg ttcaaattca ttgtaaaaca catagacaat acgtacttat
     1861 tcatacttat aattcatcat tatcaaaaaa ataacgagtt acgaaaaagt aacccgcttc
     1921 aaatcatatt tactatcctt attaatccgt ttcattttca aattgagtta aagcatcttt
     1981 aatgtcctgg tcaccactaa taatttgaaa ctcttggtga ttaaaatgat tggatgtgac
     2041 aatttctttt aatactgtcg caacatcttc tctaggaatt tcacctttac cttcaaaata
     2101 ttgtgctgct tctatctttc cagatcctgc tgcatttgta agtgcccctg gatgtaaaat
     2161 tgtataattc aaacctgaac gtcttaaata gtcatcagcg taatgtttag caattgtata
     2221 tggctttaaa tcaccgctat catcaaaagc ctgacgtctc gaatcatatg ttgaaaccat
     2281 gacatagtgt ttaatattag cctctttact cgcaatcatt gatttaacag caccatctaa
     2341 atcgacaata attgttttat ctgcacccgt attccctcca gaacctactg aaaagataac
     2401 tttatcgaat ggtttaaacg tctcagttaa agtctctatt gaatcatttt caacatcaac
     2461 aagaattgct ttcatacctt gtgattttaa cgcattaagt tgatcggatt gcctaacacc
     2521 tgcagtaaat ggtacatttt cttttgctaa ttgttgcact agtaacgaac ctacaccgcc
     2581 attagcacct ataaccaaaa tattcattta caacactctc ctatttatta ttccctatgc
     2641 cataccactt tatgagatat gtaaaacttg ttacaactat aaaaatcaat tgacatacta
     2701 ctgggaacgt attaaattaa tatatgaaca aatattcata tgaaaggatt gtcatataat
     2761 gtcagaacaa tattcagaaa taaatacaga tacattagaa cgcgtaactg aaatcttcaa
     2821 ggcattaggc gattacaatc gaatacgtat catggaattg ttatcagtca gtgaagcaag
     2881 tgttggtcac atttcacatc aattgaattt atctcaatca aatgtctcgc accaattaaa
     2941 attacttaaa agtgtgcatc ttgtgaaagc aaaacgacaa ggccaatcaa tgatttattc
     3001 attagatgac atccacgtag caactatgtt aaagcaagcc atacatcacg cgaatcatcc
     3061 taaagaaagt gggttataat atgtctcatt cacatcatca tcatgaccat atgcatagtc
     3121 atgtaactac aaataataag aaagtattgt ttatatcgtt tttaataatc ggtctatata
     3181 tgtttatcga aatcatcggc ggtctccttg ctaacagctt ggcattacta tctgacggta
     3241 tccatatgtt tagcgacaca ttctcattag gtgttgcttt aatcgcattt atatatgctg
     3301 aaaagaatgc cacaactaca aaaacatttg gttataaacg tttcgaagta ctcgcagcgt
     3361 tatttaacgg tgtaacgctt tttgtaataa gtattttaat tgtttttgaa gcgattaaac
     3421 gtttctttgt tccttctgaa gtccaatcta aagaaatgtt aatcattagt attattggtt
     3481 taattgtcaa tatcgttgtt gcattcttta tgtttaaagg cggcgacact tcacacaatt
     3541 taaatatgcg tggtgctttt ctacatgtta tcggagactt attaggttca gttggcgcca
     3601 ttactgcagc tattttaatt tgggcatttg gatggacaat cgccgatcct atcgcaagta
     3661 ttttagtttc cgttattatt ttaaaaagcg cttggggtat cacaaaatct tcaattaaca
     3721 ttttaatgga aggcacacca agtgatgttg atatagatga agttatagct actattaaaa
     3781 aggattcacg aatacaaagt gtgcatgatt gccatgtttg gacaatttca aatgatatga
     3841 atgcattaag ttgtcatgtt gttgtagacc atacattgac aatgaaagaa tgtgaattat
     3901 tattagaaaa cattgagcat gatttattac atttaaatat tcaccatatg actattcaat
     3961 tagaaacgcc taatcacaaa catgatgaat cgattatatg ttcaggaaca catagtcatt
     4021 cacatagcca ccatgctcat catcacgcgc atgtacatta ataattttaa cctactgcca
     4081 ttgcatcgat taaacttttc aatggcagta ggttttttat gtctttatgg cgacttattt
     4141 ggtctttgat gatgcaatgt ttattaacaa attttcaact attatttctt acattagtca
     4201 tatttttgac aatttactat tataattctc taactttagt cactttaatt aatttttatt
     4261 agatattaat atgaaaataa cgtgtttttt gttattgtat ttatatgaag aaaaggaggc
     4321 accactttgt ttacttactt taaatcagca tttaaaaatg ctaaaccaca attattaata
     4381 acattgatat atgcacttat agcatttgct gtaattgcag ttgtatattt acttgctaat
     4441 ttccagcttg ctaagtatgt acaaactatt gcaatttatt cacaatttgg gcaaaaacca
     4501 ccagtagatg catatttaaa agttatcgct gtattactta tagcagctgt agtttcactg
     4561 tttgttttag ttcaaatttt tatcggaatt acaaatgtta tgaaacgagc aatgagtcat
     4621 gaaaaagtaa aatttaccga tttatttatt gcatttaaaa aaggtaatta tttaaagagc
     4681 gttctgatcg gccttgtatc tatagcaatg attatcgttc tttcacttct gacatcatta
     4741 ctatacaaat tattttctcc agtatcagaa atgattatga attcagtaca atcatcatat
     4801 gcagatagta cacacctggt tggtattgct attacaactc aaagcatcat aatcattgtt
     4861 gttttattga ttaaagcaat tattacttgg ttattgttaa tacctatttt caactttatg
     4921 actagtttcg ttgaatcaac taacgataaa gtaaaaacac acttagctaa tggatttaaa
     4981 gcaatgaaaa atggtcaaaa aacatttttc aaatttttca ttggaatact attattaaac
     5041 ttagttatta ttttatttaa aacacctgtg ggttacttga tttcatttaa tacgcaatca
     5101 ctctctcaaa gtgttgcaga aaatatcata agagtttata cagtaataac aatcatttta
     5161 ttcgttgtta ttcatgcagt cattttaatg ggtatcgttc aatattactt aaaacgtggt
     5221 caaaaaatta ctaaagataa agtaaaaaca gctgacaaga ataaaaaagt tgtcactgaa
     5281 ccaaaaaata caaaagtaga aaatgataaa gtgacaacat ctgttgaaac gaaaacagaa
     5341 aaagcacaag attcattaaa tgataatact acaaaaacaa tgacttcaga taaaccagaa
     5401 gacaaccaac caaaataaat ttta
//
LOCUS       DAMZPP010000160         5368 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMZPP010000160 DAMZPP010000000
VERSION     DAMZPP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.161"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..249
                     /locus_tag="POH73_002462"
     CDS             <1..249
                     /locus_tag="POH73_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDI6888376.1"
                     /translation="LRNLFKEEGHEPWTSCEFDFTRDGKLNVSFDYIDWANSEFGPMG
                     REHYYMYKKFGIWPEKEYAINWVEKIKDYVKEQEEAEL"
     gene            260..748
                     /locus_tag="POH73_002463"
                     /pseudo
     CDS             260..748
                     /locus_tag="POH73_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
     gene            759..1247
                     /locus_tag="POH73_002464"
                     /pseudo
     CDS             759..1247
                     /locus_tag="POH73_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
     gene            1869..2259
                     /locus_tag="POH73_002465"
                     /pseudo
     CDS             1869..2259
                     /locus_tag="POH73_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
     gene            2393..2791
                     /locus_tag="POH73_002466"
     CDS             2393..2791
                     /locus_tag="POH73_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDI6888377.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLFLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLVSGIKLLTNNKPKDEISDLS"
     gene            complement(3041..3865)
                     /locus_tag="POH73_002467"
     CDS             complement(3041..3865)
                     /locus_tag="POH73_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDI6888378.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(4116..>5368)
                     /gene="brnQ"
                     /locus_tag="POH73_002468"
     CDS             complement(4116..>5368)
                     /gene="brnQ"
                     /locus_tag="POH73_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDI6888379.1"
                     /translation="GNLIFPPMLGHTAGQNMWIGMLGFALTGILLPFITVIVVAFYDE
                     GVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAYEIGTRHILPVHNQWTLIIF
                     AAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSIAVIFNPESVLSAPKDKYIT
                     HPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRMKILKYVCFSGLIAAILLGM
                     IYFALAYVGASTAPGNFKDGTDILTYNSLRVFGSFGNLVFGMTVILACLTTCIGLVNA
                     CATFTKKHVPKFSYKIFALVFSIIGFLFTTLGLEMILKIAVPLLTLIYPVSIALVLIS
                     FANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGVILKWFMMLPLADIDLAWLV
                     PFMLFAIIGFIIDVFIRRPKQATT"
BASE COUNT         2091 a          711 c          927 g         1639 t
ORIGIN      
        1 ttaagaaatt tatttaaaga agaaggacat gaaccatgga catcatgcga atttgatttt
       61 acaagagacg gcaaattgaa tgtttcattt gattatattg attgggcgaa ttcagagttt
      121 gggccaatgg gaagagaaca ttattatatg tataaaaaat ttggaatttg gcctgaaaaa
      181 gaatatgcca taaattgggt tgaaaaaata aaagattatg ttaaagagca agaagaagct
      241 gaactatagg ggagataaca tgactttcga agaaaaacta agtgaaatgt acaacaagat
      301 tgcaaatgag attgttggca tgatacctgt agaatgggaa aaggtatata caatagccta
      361 tgtaaatgat agaggtggag aggtcgtttt taattatact aaaccaggta gcgatgaatt
      421 gaattattac atgaatatat ctagagatta taatgtttcg gaagaaatat ttgatgattt
      481 atggatggaa ctttatagat catttaaaaa attaagaaat atatttaaag aagaaggaca
      541 tgaaccatgg acatcatgcg aatttgattt tacaaacgaa ggtaaattaa aagtttcatt
      601 tgattatatt gattgaaaga atacagaatt tgatcaattg agtcttgaaa attattatat
      661 gtacaaaaaa tttggggtta taccagaaat ggaagaaatt aaagaaatcg agcaatatat
      721 taaagagcaa gaagaagctg aactataggg gagataacat gactttcgaa gaaaaacaaa
      781 gtgaaatgta caataaaatt gcaaatgaga ttagtgggat gataccagta gagtgggaaa
      841 aggtatatac aattgcctac ctagatgatg aaggtggaga agtcgttttt aattatacta
      901 aaccaggtag cgatgaattg aattattaca cggatatatc tagagattat aatatttcag
      961 aaaaaatatt tgatgattta tggatgaatc tttattactt gtttatgaat ttaagggatt
     1021 tatttaaata agaagattta gaaccatgga catcatgtga atttgacttt acaagcgaag
     1081 gtgatttaaa cgtatctttt gattatatag attggattaa attaggtttt ggcccatcag
     1141 gaaaggaaaa ctactatatg tataaaaagt ttggtgtttt accagaaatg gaatatgaaa
     1201 tggaagaaat tcgagcagta gagaagtatg ttaaagagca agagtagcag acatgttata
     1261 aaaggctgtg caaaaccaac ctcgttttac atttgattca aagaagaagg taaaaaataa
     1321 gattatttgc aacttaaaaa gtcaattagc ttatcggtat ccatacatca tggataaata
     1381 agttcaacta attaacaaat cacgatataa atttaaaatt gtcattaatt gaaaaggtta
     1441 ttgtagtgta ttttataaag ttgatgtaca tcgacttatt ttttaaaact tatatcaaaa
     1501 aaatatgaga aaagtatata ctattaaaat gttttttact ataattataa atagttaaat
     1561 atttgggtat aaaataaatg tgtgggagca atttttagag atacatagca atgtaaaggt
     1621 atcaaatact tattgcataa tttattatga tgacataatt ttcagtagat agttgtaaat
     1681 taacactgaa atagatgttt atattaagaa gactttaata tcataattaa tcaaaatctt
     1741 gcgtcaaaca tttaaacgaa ttttaagttg ataacaattg gctaaacatt gttcaaacat
     1801 cacaatgata aagcatatta tcagtattgt agtgtgtgga aaatgacagc catctaagga
     1861 gaaaaatgat gaaaagaata ttggtagtat ttttaatgtt agcaattgta ttagcaggtt
     1921 gttctaataa aggtgaaaag tatcaaaaaa gatattgata aagtgtacaa agaacagaat
     1981 caaatgaata aaattgcctc gaaagtacaa aacactatta aaacagacat taaacaagaa
     2041 gacagtaata cacatgtcta taaaaatggt aaagtcattg ttattggtat tcaattgtat
     2101 aaagatcgtg aaaaaatgta ttatttcgca tatgaaataa aagatggtaa ggcagagatt
     2161 aatagagaaa tagacccaat taagtatatg aaagaccata aagcagatta tgaagatgaa
     2221 aatgtagaag tggaaaaaaa taacgtatgc tttctttaaa acttttaacc agtttcaaaa
     2281 aataaatgag ctttagcgat aataattttt acaataaaca cgatataaat ttaaatattt
     2341 atatataaga acacaaacgt aagtttgtaa ataataataa aggagtaagg caatggaaaa
     2401 atcgatcaaa ataatgacaa taataggaat tgttgttcaa ggtttagcaa cggtatttag
     2461 tttactattg atggttttag cagcatcagg tgtaatgact acagatgtgt caacaacagt
     2521 taatggtgag gttgacccag ttgatgcaga aacagcagca gcaattttca ctgtattatt
     2581 cttattccta ttcatatttg gaatcatttc aattatttta ggtgcaatcg gtatgtttaa
     2641 agcatctaaa aacaaaaaaa tgagtggaat attgttgatt attggagctg taataagtgg
     2701 taacataatt acatttgctt tatggttagt cagtggtatt aaactcctta ctaataacaa
     2761 gcctaaagat gaaataagcg acttatcata aacatcgtat attgaaattt caagatttct
     2821 taagtaatta tatttctgtt agtttttaat aaattaacca attaaataaa ttgctctctt
     2881 agaagtagat tttcagtcaa taactgcttt taagagagca ttttgtattt gataatgctc
     2941 atagcatcgg acaaaaacca ttgacgaact ttaaaaaaat acgttagaca atgcacttct
     3001 aaatattaaa ggcatcgact aacgcattgt tcaaatagag ttaattattt ttataaaatt
     3061 gatgatgatc attcaagtaa gcatagaata aacctataat gagaccgcct ccgatatagt
     3121 taccgataaa agcggcagca atatttgaaa tagcaggaat gaagtgcaat gtatcaactt
     3181 gataaattaa gcctcccata aacaagcaac tgttgtaaac gacatgttca taacccataa
     3241 aggcgaatat ggtaacaccg aacatcatga caaacatttt tgcaagtaca tcgtcaattt
     3301 gcatggcgat aactaatgaa atattaataa aaaagttggc gaatatcgct ttcattaata
     3361 tacttacaaa cccagtagac aacgttttat gttctataac tgctgataat tgatttaaca
     3421 tatctggcgt cattacattt gaaaaacgca tgaaactaaa taaaatagca gcacctaaaa
     3481 tatttcctgc aaagcacaat aaaaatattt tcaatactct agtcggttta attactttat
     3541 aatacaggcc tacagtaaag tacatgaagt tactggttag tagttcggag tttgtaaata
     3601 aaatgagtac taacgcaaaa ctgaatgtaa tggcgctggc catattcaca atgcctggcg
     3661 gtaaatctgg ttcgtgtgtt gctttaactg ataatacgaa gaccgtaata atcccgataa
     3721 taaatcctgc catcatcgcg cgtaatagat aacgttttaa ataaacgctt tgtaatatat
     3781 ctttcgttct tatcgtttcg actacgttat ttacccagtc gtccccataa aatattttat
     3841 cccatttaat atgtttctcc ttcacaatga catcctcgct tttcatttgt taatcagagt
     3901 atactatgaa tcgccttatt tgtgaataat ttcacaaaat taatttcaca aaattaattt
     3961 taaaaactaa tatttttgaa taacagctag gattttttaa caaatcattt attttaaaat
     4021 aattaataga cagacggtat cactgaaaag tcatgaattt catataaaaa agcctagtaa
     4081 cgatgggtca atcattacta ggcagtgagc atttattaag ttgtcgcttg tttcggacga
     4141 cgtataaata catcgatgat gaaaccgata atagcaaaga gcatgaatgg tacaagccaa
     4201 gctaaatcga tatctgctaa aggtaacatc ataaaccatt tcaaaataac accgtgtaat
     4261 aagttgaaac tatttagtat ttgtaaaatt gaaataatca atgtaataac agttgcgagt
     4321 cgataggccc aactgaatct gaatgtgcta aacatgttag caaatgatat cagtacaagt
     4381 gcaatagaca cgggatatat taatgtcaac aatggtacag caatttttaa aatcatttct
     4441 aaaccaagtg ttgtaaataa aaatcctatg atggagaaaa caagtgcgaa tattttataa
     4501 gaaaacttag gtacatgttt cttagtaaat gttgcacaag cgttgacgag tcctatacat
     4561 gttgttaggc atgcaagaat aaccgtcatt ccaaatacga ggttaccgaa cgaaccaaat
     4621 actcgtaatg agttgtacgt caatatatct gtaccatctt taaagtttcc tggagctgtt
     4681 gatgccccaa cgtatgcaag tgcaaagtaa atcattccaa gtaatatggc tgcaataaga
     4741 cctgaaaagc agacatattt taaaattttc atgcgatctg tgaggccttt aaacttatag
     4801 ccattgacaa tgactacgga aaaagctaac gcagcaacaa gatccattgt aaaatagcct
     4861 tccaaacttc ctgaaatgaa aggatgtgtt atatatttat ccttaggtgc acttagtaca
     4921 gattcagggt tgaaaatgac agcaatactt aatagagcga ccattaatag taataacggt
     4981 gttaataatt tacctaaatt atcaacgatt ttcgatggat ttaaactaat ccagtaaacg
     5041 atagcaaaaa agattgctgc gaatataatt aaagtccatt ggttgtgcac aggtaaaatg
     5101 tgtcttgtac caatttcgta cgcgacattt gcagcacgtg gaataccgta aaatgctccg
     5161 atagacatgt aaatcacgac agcaaaaata aacccgaacc atggatgtat acgattgcct
     5221 acactttcaa caccttcatc ataaaatgca acaacaataa cagtaataaa ggggagtaat
     5281 atgcctgtaa gagcaaagcc tagcatacca atccacatat tttgacccgc tgtatggccg
     5341 agcatgggcg ggaatattaa atttccgg
//
LOCUS       DAMZPP010000161         5246 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMZPP010000161 DAMZPP010000000
VERSION     DAMZPP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.162"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..760)
                     /locus_tag="POH73_002469"
     CDS             complement(<1..760)
                     /locus_tag="POH73_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6888380.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDM"
     gene            968..1606
                     /locus_tag="POH73_002470"
     CDS             968..1606
                     /locus_tag="POH73_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888381.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVNFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADALKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(1852..3789)
                     /locus_tag="POH73_002471"
     CDS             complement(1852..3789)
                     /locus_tag="POH73_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HDI6888382.1"
                     /translation="MTTTFIISYIVLALIIVGVINLLLIRSRRKAKRQQKEQHFATRQ
                     SNQSKFKASDLDRTTDQSSQHTAREEARIDNKDNQNQVSLNKQTEGSEQDQASFTDNN
                     ESDEEVYAAKNPNSEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTL
                     LNVALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFT
                     IGSLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMI
                     LAPAIGPTLSGYIVQNYHWNVMFYGMFIIGILAILVGFVWFKLYQYTTNPKADIPGII
                     FSTIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKAPMLNLEVLK
                     FPTFTLTTVINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFA
                     GKLLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMV
                     TAAINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNP
                     VVQDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQ
                     SNKKAKATAQKIDADNSINHE"
     gene            complement(3802..4449)
                     /locus_tag="POH73_002472"
     CDS             complement(3802..4449)
                     /locus_tag="POH73_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDI6888383.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYITTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVATVTVQGQDGETKDMELKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASVKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLDSEPSKQVVPGMNAEVKIHKN"
     gene            4570..5124
                     /locus_tag="POH73_002473"
     CDS             4570..5124
                     /locus_tag="POH73_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6888384.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKRNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQKIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQNQ"
BASE COUNT         1761 a          922 c          752 g         1811 t
ORIGIN      
        1 tcatatcagg atgtgtcatt tcacgtccac caccaaacat ataaactggt ggtaatcctt
       61 ctattgtgcc attaattggt gatatgcgct tatctgttaa tggtaggcca ttcgcccatt
      121 ttttcataat ctcattgaca ccaaactgac ttagaaccgc atcttgttcg attaaagcat
      181 ccgaaatatc tttattagat agtgttgcat ctaaaattgg tgagattaaa tacaatttat
      241 tcggtaatgg ctgttgattg tctaaaagag attgtacaaa ggataatgcc agtgcaccac
      301 ctgaaccatc acccatgact acgacatttt gatggcctac ttcagatact aattgatcat
      361 aaacacgttg tatcgcttgg aaagtgtcgt caatatgaaa ctctggtgtc tttggataga
      421 taggcagtac aacctcatat aatgtactta aagtgatttt atccaacaat ctccaatgga
      481 acggtgatgg ttgtagtgca ttgaatccac cgtgaatata taaaattttc ttatcaattt
      541 gatgtctgaa attaaagcga aagacttgca tatcatccaa tgataatttt tctaaatttg
      601 ctttaacatt taatgttgaa ggctgcttat gtttttttct attttcaatt tctcttttat
      661 aaaaaaatct ttcaacatct tgatcatttt taaacataat cgagcgattg tgaagcaaat
      721 atttattgac aacgctattc ataacacggt ttctaatcaa tgtcttaacc tacctttata
      781 tattttatgt atccaatgat tgtctatccc ctacattctt tgccaaaaaa agtatataat
      841 gtagaagata ttttcttttt cactttcaaa tttaagacta caattgaaca gtgatttttc
      901 atcattataa cagacaacta gacatattga taagtaaaga aaagaacttt atacggaggt
      961 gccttgtatg acaaatccaa atcaacgatt agaaccattt gatgagacat ttcaacaacc
     1021 gaatattcat cgtggtaagc gatatggtaa gaaaaaacgt tcattggtaa gcatgattat
     1081 tcaaatcatt gttgttatat taaccgcaat cgctggatac agcatgtgga aacaacccat
     1141 ttttaatatt gtcttcacca aagaatctgt aaattttgat ggcattaaaa attttaacga
     1201 gacaattgga aaaatgcatc aattaaatat taatttgggt aatatcaatg atttacaaca
     1261 aggaatcgat cgcttaattt tagtctttaa cggtttcttt attttatgta tcgttagcct
     1321 agtcataaca atactgacaa ttgtatttaa tcgcacagtt ctaaaagtag taaatatttt
     1381 agtgttaaca attatgttag tcattacatt gtatttcagt tatattatta aagcactcgc
     1441 acaagaaatt gcagatgctt taaaaaaata ttatttaaat gtaccaccag atcaagtgat
     1501 tacagaagct gacgctattc ataacggcat cattttactg ggctgtagca ttggtttact
     1561 tgttataagc ttattcttcc gtaatcgcat gcctcgatta aaataatgtt atatagtagg
     1621 gattgggaca gaaatgttat tttcacaaaa ttcatttcgt tgacccactt cctgcttttt
     1681 ctttattaat gacctactac atcaatgcca aattttaata taatttctct aatattctac
     1741 aaatcaacaa ataaaaaagt atcgataacc atatattggc accgatactt taaaaatcat
     1801 gtccccacaa cataattttt gattagtcac acttcaatta acttatttta tttattcatg
     1861 attgatacta ttatctgcat ctatcttttg agccgtagct tttgcttttt tgttactttg
     1921 taaaaataag cataaaatta aagcgatgat gctaaatatc gttgcaacta taaatgcatc
     1981 attaatccct tcaactgttg ctagtttatt tacaaattgt aatagcactt tcattgcgcc
     2041 ttcttgacca ccatattgcg atgctaattc acgcatatga tcttgtacaa caggattcgt
     2101 tttatctaac tcttccccaa aagctgataa atgttgtgta gtttgcgttg tcattacagt
     2161 aaccaagatt gctgttccta tagaaccagc tagttgacgc atcgtattta agaaggcatt
     2221 accatgggat gccagtcgtc ccggcaacgc attaatagct gctgttacca ttggcatcat
     2281 aataaatgcc ataccaaatg aacgtagtac atagatgccc atgattgtca tatatggcgt
     2341 atccatattc aatttagtta attcccatgt tgcataagtc atgacagcaa taccaaaaat
     2401 ggctaaaggt tttaaaccaa tcgtgtctaa caacttacca gcaaatggtc ctagtagacc
     2461 cataattaga gaaccaggta ataataacaa tccagaatct aatgctgaga aaccacgtaa
     2521 attttgtaag taaattggta ataaaatcat accaccatac aagctcaaca ttacaaccat
     2581 attaattact gttgttagtg taaatgttgg aaatttcaat acttctaaat tcaacattgg
     2641 tgctttcatt cttaattctc ttattacaaa tagaataata aagataatac caatcgcaaa
     2701 cattgtttct atctcaacag aaccccagcc tttgttcccc gcttctgaaa agccgtataa
     2761 caaagcacca aaaccaatcg tactaaaaat gatgcctggg atatcagctt tagggtttgt
     2821 tgtatattga tataacttaa accatacaaa accaactaag attgcaagaa taccgataat
     2881 gaacataccg taaaacatca cattccagtg gtaattttgt acaatataac ctgataatgt
     2941 tggaccgatt gcaggtgcta aaatcattgc gatacccatt gtacccatgg cagcaccacg
     3001 tttttcaggt ggataaattg taataataac aattgaacct aatggcatta gtacacctgc
     3061 accaatggct tgtaatacac gtccaaccat catgattggg aaattcattg aaatcgcaca
     3121 tattaatgaa ccaattgtaa agagtactaa cgcaactaaa aataattttc gatatgaata
     3181 tttattaaat agatacgccg taattggtat taaaataccg tttactaaca tgaatcccgt
     3241 catcaaccat tgccctgttg acgcagaaat attaaattcc gtattaattt ttggcaaagc
     3301 aacatttaat aatgtttggt ttaaaatcgc aataaacata ccgaataata atgctgctaa
     3361 tattttaccg cgtgaaacac cttcaccaaa aataaagttt ttatgttctt ttttaatttt
     3421 ctcatttact ttatattctt ctgagttagg atttttagca gcatatactt cttcatcaga
     3481 ttcattatta tcagtgaatg atgcttgatc ttgttcagac ccctctgtct gtttgtttag
     3541 actaacttga ttctgattgt ctttattgtc aatacgtgcc tcttcacgcg ccgtatgttg
     3601 tgacgattga tcagttgttc tgtctaaatc acttgcttta aatttagatt gatttgattg
     3661 acgtgtcgca aaatgttgtt ccttttgttg gcgttttgct tttcttcttg atcttattaa
     3721 taataaattg ataacaccaa caataatgag cgctaaaaca atgtagctaa taatgaaggt
     3781 cgtagtcatt taatgacccc cttaattttt atggattttt acttcagcgt tcattccagg
     3841 aacaacttgt ttagacggtt ctgaatctaa cgtaatttta acaggtataa cttgagatac
     3901 tttagtatag ttaccatcac tatttgatga tggcattaat gaaaaactag cagcagtagc
     3961 ttttccaata ctatcaactt tacctttaac agaagctttt tgaccgtcaa tagtcacatc
     4021 aacatcttta ccaacttcaa catctttaat atctttttcg tcaatatttg cagttacata
     4081 taaatcatct aaattgtaag cataagcaat tggattgcct gcttgtacca tagaaccttc
     4141 catgccatct aatttagcaa ttgtaccttt ttgtggcatt ttaagttcca tatctttagt
     4201 ttcgccatct tgaccttgaa cagtaactgt tgctacttta tcacctttat caagtttatc
     4261 accttgctta acatttagtg atttgatttg cccagaagct ggacttgcta ttttaatttg
     4321 atcgccattt actttagcat tatcagttgt tatataactt gttgttttat tccagaaata
     4381 aaaaccagca atcccaattg ctagcagtac aacaaccgta atgacattaa ttaatatcat
     4441 ctttttcatg aaacatttcc tcctatttgc ttttaaaatt cactttattt attatagaga
     4501 catgataact aaaaaaacac caacaatttt tataagagtg ttggttaaaa ttcacaaagg
     4561 tgtaacaata tgaaacgaca agccaaaata gaaattcaaa acgcacttgt tgatttgatg
     4621 gctgaatatc cttttcaaga aatatccact aaaatgattt gcgcgtattg taatattaat
     4681 cgttctactt tttatgatta ttacaaagat aaatttgatt tattggatac catcaactca
     4741 aagcataaag aaaaattcca atttcttcta agcgcgctac atcataattt cgaaaatatt
     4801 aaacaggata agcttaaact ttataaattc ttcattatta ttgctaagta tatcaagcgc
     4861 aatgagcagt ttttcaaaga cattttagtt acctatccaa tgaaaacatt attcatagac
     4921 tatataaatt tagcgagaga ttattatcaa aaaattatga atgactatag tactacagta
     4981 accaataaac agctttatgt tacatatatc ataggcggtc aagctggggt atttattaat
     5041 tggctttcga atggttgtaa tgaatcacct gaagaagttg ctgacatttt attggctaat
     5101 acgattaaac ttcaaaacca ataatacggt ttcattctaa taaagaaatg ctataaaaca
     5161 actagataaa gttaaaccaa atggacagat aatagtacat atgtaatggt gctatcaata
     5221 taaaaatgcg cttgggacat taagct
//
LOCUS       DAMZPP010000162         5164 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMZPP010000162 DAMZPP010000000
VERSION     DAMZPP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.163"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(188..544)
                     /locus_tag="POH73_002474"
     CDS             complement(188..544)
                     /locus_tag="POH73_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDI6888385.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     ATIEKMISEKDESIEKNVKLPSDADVSVKKGDFVIVYKLADSDKIIKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
     gene            complement(822..1244)
                     /locus_tag="POH73_002475"
     CDS             complement(822..1244)
                     /locus_tag="POH73_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDI6888386.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMIFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL"
     gene            complement(1315..3420)
                     /gene="rsp"
                     /locus_tag="POH73_002476"
     CDS             complement(1315..3420)
                     /gene="rsp"
                     /locus_tag="POH73_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HDI6888387.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKKAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPTSDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
     gene            complement(4908..>5164)
                     /locus_tag="POH73_002477"
     CDS             complement(4908..>5164)
                     /locus_tag="POH73_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDI6888388.1"
                     /translation="QMIDDALLIEPSIGSVCNAFDHMWGYFKKCANEEERQQSKLLKA
                     DFINGKIDTQTLLGFLAELANKYDVQYLLQSRVLNTKRKR"
BASE COUNT         1861 a          764 c          709 g         1830 t
ORIGIN      
        1 tgcatgcgca aagcgcaggc gaactgaagc gaagtgtagg ggcttttatg catgcgctaa
       61 agcgcagggc atttgcaaat aaactggtat atttattctc aaaaaaacag cagtaagatg
      121 attttcaatt agaaatatct tactgctgct tttaatatct tatatattac tcaaaattaa
      181 agcttaatta gtgtttcatg tgcattttgc ccatgtcatg aattttcatc attaaaccat
      241 gtggtacatc gtcatgatca acttttttta ctttaataat tttatcagaa tcggctaatt
      301 tataaacaat tacaaagtcg ccttttttaa ctgaaacgtc tgcatctgaa ggtaatttaa
      361 cgttcttctc aatgctttca tctttttcac taatcatttt ttcaattgta gcattgtctt
      421 tcataattcc ataatatgta tctttctttc caccagtcat catcatgatt actagtacaa
      481 tacatacaat caacatgatg gcaattaaac taataccaag accaaaacct tttttctttt
      541 tcacggttca tcaatctcct taaatgtagt taattctgtt atacctaaaa gtacaacagt
      601 ttgcaaatgc agtatttata ttacataaaa ttaatttaaa aagacacacc ttaatcacaa
      661 taaatgtgaa taaaatgtgt ctaaaatatt gttatataat aaaaatatag ttaaaagtat
      721 taaacgcttt caaaatcgaa atatatataa ataggatact gttgaaattt atagctaaca
      781 tgttcaattg ctgactaatt ttaaaactaa aaacttacat attataaatc aattttaata
      841 ggtgtatcta atgatttatc attcattagc ttaatggatt gtggaactac tcttaaaaca
      901 cataactctg ggtcttcttt tgaactgaaa aagcttttgt cttgtgtttc ccatagccaa
      961 tcaatcactt cttgttctgt aacgatttcg attgtcgctt ccatttcaac gaaactacga
     1021 ttagttgtgt cgttatagcc taataaaaca tatgctgctg gattatcttt aatttctttg
     1081 accttagcag aatggatatt cgttttagta tataaagtaa gaccatcatt ataaaagatc
     1141 atatatctac tattaggttt gttattatat gcagttgata atacaccaac ttttgacgtt
     1201 gctagcacgt tttcaattgc ttgaattgct tgtgagttac tcatcctata tcatctcctt
     1261 atgatataaa tagcctttaa cgtaagaaac taaacacaag aggactgtta gtatttagct
     1321 tggtttaaag caaatatatt taacctcgtc gtgatttaac ttgagttcaa atgtacgacc
     1381 agtttcttct tgaactgcaa gctccgtttt agggtaattc gttttgtcta attcatctag
     1441 aatttctttt tctatataga caagttgatc gcttgttgga agcaaatgtt gaattgaacc
     1501 atgttcatga ttcaatgttt taataatcat caaagagtct tgaggtaatt cattatggaa
     1561 aatgaagtct tgttttacgt ctgacatata tcggtcatta attttattga ataataagaa
     1621 atggtagtta ttatctttac gactcaatac ataattattt gtaatttcaa tgtcttcatt
     1681 aacaaaaggt ctaaacattt tatataaatg cattaatggt agggggctac catatttgtt
     1741 gtacaaggaa atataatttg aatggtcatc taataattga tagctaatgc caccgccata
     1801 tcgcaataat gcgattaagt gatacactat gtcagataat tgaattggat tcttaccatc
     1861 attagtatat ttaaatccac ttacagacag gttattatat acaaattgtg ttgatgatat
     1921 acctgaatct aaaataaatt tcttatactg ttcaaaatga gtgtcagttt gatgatagtg
     1981 ttggttttta gtgataagat gtgttgcagt ttttgaattt tcaatatcaa taaagtaata
     2041 atggaattta agacgctgca tgacatcata aacttgttcc acggttgaat aagtttctaa
     2101 aaagccatcg actgtcacac tatatttaat ttctttattt ttgtttttaa ttttaagatg
     2161 acataaatga atttcttctg ctgtcataga gttagagcaa aatacgagca taaatttaac
     2221 taatttcttg cttgtcttat agtcttgatt gccttgtaaa aatgttagta ttaattgctt
     2281 catcgtttcg aattgttgtg ttgatttaat agcgattgct aaacctatat ttttttcgaa
     2341 aagcttttca aaacatctat ttaacaggtt gtaattcgtt tggctaatct caatatcgtg
     2401 aatatcgtca atgaaaacga caggttttgg taggtgctca aagttaatat cgtaatattc
     2461 attgaaaata aattgaaata gttcattgaa attactaaat cgaacaaata ctttggcacg
     2521 ctgacttcga tctttcggat taaaatcatc tatatgaata tctgttgtat ttgtagccag
     2581 ctgagctgtt aaatcaattg ttgaaattaa ctcagtgaat tgtgatacgt tatcttgttg
     2641 gaagtgtatt tgtggtggtt cagtaatctt ggaaacgagt gaacggaact gtttaggact
     2701 aaaatgcaag taattcttaa attgatttgc aaaatttgta tgactgttaa aaccggccaa
     2761 ttcagaaaca gttgtaatag aatgttttgt agaaagtaat aaattgatgg cgttaacaag
     2821 tttaatactt gtaaaataat ctttaaaatt catacttaaa tatcgaacaa aaagattaga
     2881 acaatatgat tcagagatgt tgcaatgcat cgctatatct tttaaagaca agtgcgcatc
     2941 aatattgtca tgaatatagt ttacacagtc tataaacacc ggattgctta aagcaatatt
     3001 tggtaaatat tcatgatcta ttcttataaa agctttttta agtaaagtgt ctataatact
     3061 ttgacctata ttttttccca ttaaagaaga acttgttggt gtactgatta aatcggcaat
     3121 taatgactta atataattac ttgattgtaa taaatgacga tctaaataac cattaaagaa
     3181 atcatcatct tgttgataaa aataaaaaac gggtatcatt aattcgatta tattttcgcc
     3241 gtggttaata ttataaatat caccatgatt aattaagata atataattac ttacgtcttt
     3301 ggtttcacca ttaatcgtaa tattgagttt acttgtaagt gaaaatagca ccataatatt
     3361 attgatatta cgacttggta ttgtcgataa tgtatgatgt attttaagtt ggcatgtcat
     3421 atttctctct cctgcctaat atatattgaa atctatcatt taaatgtaaa acatggaaat
     3481 ttttaaaatc atggatagta tacctattat agttgaatat ttttgaaaag gtaatagtga
     3541 aaaataaaag aaattaaaga aaactcctag gaaaacgctt gtttttatcc atcgtgcaca
     3601 atataaaagt aaaattagaa agatagtaag caaaatttgg aaattttcga tagagtctaa
     3661 ttaaattatt tggtgcataa ttaatataga tttcaaacct atgtatttca aaaaacaata
     3721 catagccatt ttgaactaac ttgctaatct tttattgata ttcaactatg ttataaaaaa
     3781 agcaaattga ccaaaatgga agtttgaatc aaaaaacatt aaatgtaccc atgacttaaa
     3841 ttttatcgcc attatttaag tttggacata cacttttgtc atcaaacgct gaaaattagt
     3901 tcgattgaaa tcacttcttg ttataaaaat tcttagtagc cctgttacaa aatattcgct
     3961 acaaattatt caaatcaaca acaataacca tcaactaaga aattttaaac aatctactaa
     4021 aaaataaagc gtttaaaagt tctgtttaga aaacatcaag ataagtataa acatctagaa
     4081 aagtgaacat ctaagaaaat ccatctagtt attgccgtaa caattagaat aatcatttga
     4141 aaaagtgcat tagacaacga aattcaccat caagataatg aagcaacaag aaacagcaca
     4201 catcaatgaa ttaacacctt agattcatca tgagaaaatg catggcacaa ctagatttac
     4261 catcaagata attcttaagc agcgaagaat taacatctag aaatgcataa acatcttaga
     4321 ttttacatca agagatgcga taactataaa caactagaaa cgtcaccaag atagtgcaac
     4381 aacaaagaat cagcatcttg aaatcgcact aacatctaag aatcaacatc aagtattata
     4441 ttaacatcat agataacttc atcaagacag gcatccacat ctaagaaact catctagaat
     4501 agctaacacc taacatcaaa gaatattcat caagaacagt atctaacaga actttttgac
     4561 tatatatgaa ttaattatgt tactagctaa atcatctatt attaactttc acactcaatt
     4621 tctacatcta atatcaattt attaacaata gaattagcag caacaagata ttacttaaaa
     4681 ttactccccc gaatttaaaa tataaacaat gtaataatta agggtataaa ttgatttatg
     4741 ctgtaactaa ttaaatgtgt atggtatctt ctaacatcat gaaacgcatt ttaatataga
     4801 tatgtacatt taaaccttga gagatagatt atagtaaact ttagtacaaa gtatgaattg
     4861 ctgaactcca atgactacta tttgtccgtt acaactaaag tttcaatcta tctctttctt
     4921 tttgtgttta aaacacgact ttgtagtaaa tattgaacat catatttatt agcaagttct
     4981 gctaaaaaac ctaataaagt ttgggtgtcg attttaccat taataaaatc agctttaagt
     5041 agttttgatt gttgtctttc ttcttcattc gcacattttt taaaatatcc ccacatatga
     5101 tcaaaagcat tacatacact tcctatactc ggttcaatta acaaagcatc atcaatcatt
     5161 tgtt
//
LOCUS       DAMZPP010000163         5143 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMZPP010000163 DAMZPP010000000
VERSION     DAMZPP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.164"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..104
                     /locus_tag="POH73_002478"
     CDS             <1..104
                     /locus_tag="POH73_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1270 family protein"
                     /protein_id="HDI6888389.1"
                     /translation="VLMPLLYFTTAWSIAGFASIATFIFYKEYFYEE"
     gene            194..523
                     /locus_tag="POH73_002479"
     CDS             194..523
                     /locus_tag="POH73_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888390.1"
                     /translation="MTENIKTEQHYYTKDFSGYRNEEDNFVANQELTVTITLNEYRKL
                     IEIKAVKDKEEDTYRGKYFAEERKNEKLEKENIKLKNKIYELQNEEDNEEDEEDKEDE
                     NDVLQNW"
     gene            504..764
                     /locus_tag="POH73_002480"
     CDS             504..764
                     /locus_tag="POH73_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDI6888391.1"
                     /translation="MYYKIGEIKNKIISFNGFEFKVSVMKRHDGISIQIKDMNNVPLK
                     SFHVIDLSELYIATDAMRDVINEWIENNTDEQDRLINLVMKW"
     gene            774..995
                     /locus_tag="POH73_002481"
     CDS             774..995
                     /locus_tag="POH73_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2483 family protein"
                     /protein_id="HDI6888392.1"
                     /translation="MKQTVTYLIKHKDENLFITNRPTEVNDTVKYSTDMRDAREFDGL
                     DKTVIDMSKHKAIKKTVTETIEYEEVEHD"
     gene            988..1776
                     /locus_tag="POH73_002482"
     CDS             988..1776
                     /locus_tag="POH73_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDI6888393.1"
                     /translation="MTEEKQEPQEKVSILKKLKINNIAEKNKRKFYKFAVYGKIGSGK
                     TTFATRDKDAFVIDINEGGTTVTDEGSDVEIENYQHFVYVVNFLPQILQEMRENGQEI
                     NVVVIETIQKLRDMTLNDVMKNKSKKPTFNDWGEVAERIVSMYRLIGKLQEEYKFHFV
                     ITGHEGINKDKDNEGSTINPTITIEAQEQIKKAITSQSDVLARAMIEEFDDNGEKKAR
                     YILNAEPSNTFETKIRHSPSITINNKKFANPSITDVVEAIRNGN"
     gene            1805..2356
                     /locus_tag="POH73_002483"
     CDS             1805..2356
                     /locus_tag="POH73_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6888394.1"
                     /translation="MKITGQAQFTKETNQEKFYNGSAGFQAGEFTVKVKNIEFNDREN
                     RYFTIVFENDEGKQYKHNQFVPPYKYDFQEKQLIELVTRLGIKLNLPSLDFDTNDLIG
                     KFCHLVLKWKFNEDEGKYFTDFSFIKPYKKGDDVVNKPIPKTDKQKAEENNGAQQQTS
                     MSQQSNPFESSGQFGYDDQDLAF"
     gene            2369..3061
                     /locus_tag="POH73_002484"
     CDS             2369..3061
                     /locus_tag="POH73_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888395.1"
                     /translation="MQYITRYQKDNDGTYSVVATGVELEQSHIDLLENGYPLKAEVEV
                     PDNKKLSIEQRKKIFAMCRDIELHWGEPVESTRKLLQTELEIMKGYEEISLRDCSMKV
                     ARELIELIIAFMFHHQIPMSVETSKLLSEDKALLYWATINRNCVICGKPHADLAHYEA
                     VGRGMNRNKMNHYNKHVLALCREHHNQQHAIGVKSFDDKYHLHDSWIKVDERLNKMLK
                     GENNGRSIVDKT"
     gene            3033..3836
                     /locus_tag="POH73_002485"
     CDS             3033..3836
                     /locus_tag="POH73_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDI6888396.1"
                     /translation="MGEVSWIKLKVGMFDDSKIKYIEALPERDTIITIWVKLLTLSGK
                     YNEQGYIMLSENLPYNEEMLANEFSRPINSIRLAIQTFETLGMIEKVNGVIKVTNWEK
                     HQNIEGLEKIRAQNRLRKQKQRENNRKLLNGHVTSRDSHATEEDKELDKELERDKEKD
                     IDKNLSSINSATDVTHEQFEEWWKLYDKKKDKKMSFTKFKSCLKKHSFEQIMQGTREY
                     LKTITDKQYQKYPKTFLTNESYMNDYSEEIKETGIDQLERMKYDESYWD"
     gene            3846..4625
                     /locus_tag="POH73_002486"
     CDS             3846..4625
                     /locus_tag="POH73_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDI6888397.1"
                     /translation="MKPLFSEKINESLKKYQPTHVEKGLKCKRCGSEYDLYKFAPTKK
                     HPNGYEYKDGCKCEIYEEYKRNKQRKINNIFNQSNVNPSLRDATVNNYKPQNEKQVKA
                     KQTAIEYVQGFSTKEPKSLILQGSYGTGKSHLAYAIAKAVKSKGHTVAFMHIPMLMDR
                     IKATYNKNAVETTDELVRLLSDIDLLVLDDMGVENTEHTLNKLFSIVDNRVGKNNIFT
                     TNFSDKELNQNMNWQRINSRMKHNARKVRVIGDDFRERDAW"
     gene            4619..4777
                     /locus_tag="POH73_002487"
     CDS             4619..4777
                     /locus_tag="POH73_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888398.1"
                     /translation="MVTKEFLKTKLECSDMYAQKLIDEAQGDENRLYDLFIQKLAERH
                     TRPAIVEY"
     gene            4790..5011
                     /locus_tag="POH73_002488"
     CDS             4790..5011
                     /locus_tag="POH73_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3269 family protein"
                     /protein_id="HDI6888399.1"
                     /translation="MPKEKYYLYREDGTEDIKVIKYKDNVNEVYSLTGAHFSDEKKIM
                     TDSDLKRFKGAHGLLYEQELGLQATIFDI"
     gene            5021..>5143
                     /locus_tag="POH73_002489"
     CDS             5021..>5143
                     /locus_tag="POH73_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDI6888400.1"
                     /translation="MSKYNAKKVEYKGIVFDSKVECEYYQYLESNMNGTNYDRIE"
BASE COUNT         2119 a          749 c         1002 g         1273 t
ORIGIN      
        1 ttgttcttat gccattgctg tacttcacta cagcatggtc aattgcagga ttcgcaagta
       61 tcgcaacatt catattctat aaggaatact tttatgaaga atgaaaaaac tgctacttgc
      121 gccaacaagt aacaataagt gttcatcaaa atatacaact taattaaatc aaaatatacg
      181 gaggtagtca actatgactg aaaatattaa aactgaacaa cattattaca ctaaagattt
      241 ttcaggatac agaaatgaag aagataactt tgtagcaaat caagaattga cagtaacaat
      301 cacattgaac gagtacagaa aacttattga aataaaggct gttaaagata aagaagaaga
      361 tacttacaga ggtaagtatt ttgcggaaga aagaaaaaac gaaaaattgg aaaaagaaaa
      421 tataaaacta aaaaacaaaa tttatgaatt acaaaacgaa gaagataacg aggaggacga
      481 agaagacaag gaggacgaga acgatgtatt acaaaattgg tgagataaaa aacaaaatta
      541 taagctttaa cgggtttgaa tttaaagtgt ctgtgatgaa gagacatgac ggtatcagta
      601 tacaaatcaa ggatatgaat aatgttccac ttaaatcgtt tcatgtcata gatttaagtg
      661 aactatatat tgcgacggat gcaatgcgtg acgttataaa cgaatggatt gaaaataaca
      721 cagatgaaca ggacagacta attaacttag tcatgaaatg gtaggaggtc gctatgaagc
      781 agactgtaac ttatctaatc aagcataaag atgaaaatct atttattaca aaccgaccaa
      841 ccgaagtgaa cgatacagtg aagtattcaa ctgatatgcg agacgcaaga gaattcgacg
      901 gactagacaa aactgttatt gatatgtcta agcacaaagc aatcaagaaa acagtgacag
      961 aaacaattga gtatgaggag gtagaacatg actgaggaaa aacaagaacc acaagaaaaa
     1021 gtaagcatac tcaaaaaact aaagataaat aatatcgctg agaaaaataa aaggaaattc
     1081 tataaatttg cagtatacgg aaaaattggc tcaggaaaaa ccacgtttgc tacaagagat
     1141 aaagacgctt tcgtcattga cattaacgaa ggtggaacaa cggttactga cgaaggatca
     1201 gacgtagaaa tcgagaacta tcaacacttt gtttatgttg taaatttttt acctcaaatt
     1261 ttacaggaga tgagagaaaa cggacaagaa atcaatgttg tagttattga aactattcaa
     1321 aaacttagag atatgacatt gaatgatgtg atgaaaaata agtctaaaaa accaacgttt
     1381 aatgattggg gagaagttgc tgaacgaatt gtcagtatgt acagattaat aggaaaactt
     1441 caagaagaat acaaattcca ctttgttatt acaggtcatg aaggtatcaa caaagataaa
     1501 gataatgaag gtagcactat caaccctact atcactattg aagcgcaaga acaaattaaa
     1561 aaagctatta cttctcaaag tgatgtgtta gctagggcaa tgattgaaga atttgatgat
     1621 aacggagaaa agaaagctag atatattcta aacgctgaac cttctaatac gtttgaaaca
     1681 aagattagac attcaccttc aataacaatt aacaataaga aatttgcaaa tcctagcatt
     1741 acggacgtag tagaagcaat tagaaatgga aactaaaaat taattaaaag gacggtattt
     1801 aattatgaaa atcacaggac aagcgcaatt tactaaagaa acaaatcaag aaaagtttta
     1861 taacggctca gcagggtttc aagctggaga attcacagtg aaagttaaaa atattgaatt
     1921 caatgataga gaaaatagat atttcacaat cgtatttgaa aatgatgaag gcaaacaata
     1981 taaacataat caatttgtac cgccgtataa atatgatttc caagaaaaac aattgattga
     2041 attagttact cgattaggta ttaagttaaa tcttcctagc ttagattttg ataccaatga
     2101 tcttattggt aagttttgtc acttggtatt gaaatggaaa ttcaatgaag atgaaggtaa
     2161 gtattttacg gatttttcat ttattaaacc ttacaaaaag ggcgatgatg ttgttaacaa
     2221 acctattccg aagacagata agcaaaaagc tgaagaaaat aacggggcac aacaacaaac
     2281 atcaatgtct caacaaagca atccatttga aagcagtggc caatttggat atgacgacca
     2341 agatttagcg ttttaaggtg tggtttaaat gcaatacatt acaagatacc agaaagacaa
     2401 tgacggcact tattccgtcg ttgctactgg tgttgaactt gaacaaagtc acattgactt
     2461 actagaaaac ggatatccac taaaagcaga agtagaggtt ccggataata aaaaactatc
     2521 tatagaacaa cgcaaaaaaa tattcgcaat gtgtagagat atagaacttc actggggaga
     2581 accggtggaa tcaactagaa aattattaca aacagaattg gaaattatga aaggttatga
     2641 agaaatcagt ctgcgcgact gttctatgaa agttgcaagg gagttaatag aactgattat
     2701 agcgtttatg tttcatcatc aaatacctat gagtgtagaa acgagtaagt tgttaagcga
     2761 agataaagcg ttattatatt gggctacaat caaccgcaac tgtgtaatat gcggaaagcc
     2821 tcacgcagac ctggcacatt atgaagcagt cggcagagga atgaacagaa acaaaatgaa
     2881 tcactacaac aaacatgtat tagcgttatg tcgcgaacat cataaccagc aacatgcgat
     2941 tggcgttaag tcgtttgatg ataaatatca cttgcatgac tcgtggataa aagttgatga
     3001 gaggctcaac aaaatgctga aaggagaaaa caatgggaga agtatcgtgg ataaaactta
     3061 aagttggcat gtttgatgac agcaaaatca aatatatcga agctttaccc gaaagagata
     3121 cgatcataac tatttgggtt aagttgctaa ctttatcagg aaagtacaac gaacaaggtt
     3181 acattatgct atctgaaaat ttgccgtaca acgaagaaat gttagcaaat gagtttagcc
     3241 gacctatcaa ctcgataagg ttagcaatac aaacttttga gacattaggc atgattgaaa
     3301 aagttaatgg tgtcataaaa gtgacaaact gggaaaagca ccaaaacatt gaaggactcg
     3361 agaaaatcag ggcgcagaac aggttgagga aacaaaagca acgagaaaac aacagaaaat
     3421 tgctaaatgg tcacgtgacg tcacgtgaca gtcacgcaac agaagaagat aaagaattag
     3481 ataaagaatt agaaagagat aaagaaaaag atatagataa gaacttaagt tcaattaata
     3541 gcgcaactga cgttacgcat gagcaatttg aggaatggtg gaaactttac gacaagaaga
     3601 aagataagaa gatgtctttt actaaattca aatcatgctt aaagaaacat tcttttgaac
     3661 aaatcatgca aggcactcga gagtatttaa aaactattac agacaaacaa tatcaaaagt
     3721 accccaaaac gttcttaact aatgaaagct atatgaatga ttatagcgaa gagattaaag
     3781 aaactggcat agatcaattg gaacgtatga agtacgacga aagttattgg gattaggggg
     3841 acattatgaa accactattc agcgaaaaga taaacgaaag cttgaaaaaa tatcaaccta
     3901 ctcatgtcga aaagggattg aaatgtaaga ggtgtggcag tgaatacgac ttatataagt
     3961 tcgctcctac taaaaaacac ccgaatggtt acgagtataa agatggttgc aagtgtgaaa
     4021 tttatgagga atataagcga aacaagcaac ggaagataaa caacatattc aatcaatcaa
     4081 atgttaatcc gtcattaaga gatgcaacgg ttaacaacta taagccacaa aatgaaaaac
     4141 aagtaaaagc taaacaaaca gcaatagagt atgtacaggg tttctctaca aaagaaccaa
     4201 aatcattaat attgcaaggt tcatatggaa ctggtaaaag ccatctagca tacgctatcg
     4261 caaaagcagt caaatctaaa gggcatacag ttgcttttat gcacatacca atgttgatgg
     4321 atcgtatcaa agcgacatac aacaaaaatg cagttgaaac tacagacgag ctagtcagat
     4381 tgctaagtga tattgattta cttgtactag atgatatggg tgtagaaaac acagagcaca
     4441 ctttaaataa acttttcagc attgttgata acagagtagg taaaaacaac atttttacaa
     4501 ctaactttag tgataaagaa ctaaatcaaa atatgaactg gcaacgtatc aattcaagaa
     4561 tgaaacacaa tgcgagaaaa gtaagagtaa tcggagacga tttcagggag cgagatgcat
     4621 ggtaaccaaa gaatttttaa aaactaaact tgagtgttca gatatgtacg ctcagaaact
     4681 catagacgag gcacagggcg atgaaaatag gttgtacgac ctatttatcc aaaaacttgc
     4741 agaacgtcat acacgccccg ctatcgtcga atattaagga gtgttaaaaa tgccgaaaga
     4801 aaaatattac ttataccgag aagatggcac agaagatatt aaggtcatca agtataaaga
     4861 caacgtaaat gaagtttatt cgctcacagg agcccatttc agcgacgaaa agaaaattat
     4921 gactgatagt gacctaaaac gatttaaagg cgctcacggt cttttatatg agcaagagct
     4981 aggattacaa gcaacgatat ttgatattta gaggtggacg atgagtaaat acaacgctaa
     5041 gaaagttgag tacaaaggaa ttgtatttga tagcaaagta gagtgtgaat attaccaata
     5101 tttagaaagt aatatgaatg gcactaacta tgatcgtatc gaa
//
LOCUS       DAMZPP010000164         5111 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMZPP010000164 DAMZPP010000000
VERSION     DAMZPP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.165"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..192
                     /locus_tag="POH73_002490"
     CDS             <1..192
                     /locus_tag="POH73_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDI6888401.1"
                     /translation="TLALNNATLPYALMLANKGYREAFKSNQPLSLGLNTYKGHVTNK
                     GVAEAFEMEYKSVEEALHL"
     gene            320..424
                     /locus_tag="POH73_002491"
     CDS             320..424
                     /locus_tag="POH73_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888402.1"
                     /translation="MILIHYMLLMNIPAYVFLACLLHIAFRLGQKFMS"
     gene            complement(502..1557)
                     /locus_tag="POH73_002492"
     CDS             complement(502..1557)
                     /locus_tag="POH73_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDI6888403.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEINKIRKAAELADKCIEIGVSYLKES
                     VTEREVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSQEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            1732..2421
                     /locus_tag="POH73_002493"
     CDS             1732..2421
                     /locus_tag="POH73_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6888404.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTEATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(2728..3141)
                     /locus_tag="POH73_002494"
     CDS             complement(2728..3141)
                     /locus_tag="POH73_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDI6888405.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            3346..4644
                     /locus_tag="POH73_002495"
     CDS             3346..4644
                     /locus_tag="POH73_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDI6888406.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVIDFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANQ
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            4955..>5111
                     /locus_tag="POH73_002496"
     CDS             4955..>5111
                     /locus_tag="POH73_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDI6888407.1"
                     /translation="MISTMNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFA"
BASE COUNT         1705 a          774 c          885 g         1747 t
ORIGIN      
        1 acgttagctt taaataatgc tacgctacct tatgcgctca tgctagctaa taaagggtat
       61 agagaagcat ttaaatcaaa tcaaccatta tcattaggtt taaatactta caaaggtcac
      121 gtaaccaata aaggcgttgc agaggcattt gaaatggaat ataaatctgt agaagaagca
      181 ttacacttat aatttaaatt tgatgaaaag ggaatgaatt acagcgtatt aaagaatgac
      241 gttatcatct ccttggttat taaaattaaa taatgataaa ttaaagctag aaacaatgcg
      301 aagaaatcat cgaacatgaa tgatattgat tcattacatg ttactgatga acatacctgc
      361 atatgtgttt ctagcttgtt tgttacatat tgcatttaga cttggacaaa aatttatgtc
      421 ctaggcgcta caatgttata ttggcagtgt ttgactgaat gagaatgcgc tagtaacaag
      481 cttttcattt ttgtcccact cttatttttc gtaatgtgtt aatacttcgt atccttcatt
      541 agtgacaagt atgtcatctt caattcttac acctgcaaca cctggtacat aaatacctgg
      601 ttcgattgta atgaccatgc cagcttctaa caaattagaa ttagtacttg aaacatcttg
      661 atattcatgt tcctgtaatc ctagaccatg acctaagcga tgagggaaat attcaccata
      721 acctttttct gaaataatat ttctagcgat atgatcgata tcttttaaag gtattccagg
      781 tttaattgct tggattgcag atgtttctgc ttctaataca atattataaa tttcttgtgc
      841 ttcttggcta ggttcaccaa atttaatggt acgtgtcata tcgctacaat aatgctcata
      901 aattacacct aaatcaaata gtacatactc attgcttttt aatctgcgat ctcctggtgt
      961 gccatgaggt gatgcggcat gatctccaaa taaaaccatc gtatcaaaac tcatttcatt
     1021 gacgccatat tgtttgattg tttgctcaat atggttgact acttcacgtt cagttacact
     1081 ttcttttaaa taagaaacac ctatttcgat acacttatct gctaactcag cagctttacg
     1141 tattttgtta atttcatctt cagatttaat atttctcaat tgtttgattg ttaaatcaac
     1201 atctccgaat gaattgacat tgaaaccaga gattaattgt ttttggcgtg ctactgttaa
     1261 gtgctcgctt tcaattagta atttattgat tgtttgagga taaagtgaaa aaggattttc
     1321 agtgtctaaa tatccaacga tttcacctgt gaaaggtgat gctttgactt cttcgacttc
     1381 catttttgga caaaatagta cttgtttacc atctttctta atcaataatg caaataatct
     1441 ttcatggggt tcgctacggt atccagtaaa ataatataca ttcaacggtg ttgtaatcca
     1501 tgctgcatca gcttgttgat tgttcagttc gtctattatt tttgatattt ttgtcatttt
     1561 ataaacactc ctcaacataa tattacttac attttatgct aattattatt agatttcaaa
     1621 atcgagctaa tacattaact agattttcaa tttttaaaat ttgagaattt gttataatta
     1681 agaggcaaaa gggtactaaa tttatatagc aattgatagg aggatgctca aatgaagtta
     1741 tcatttcacg gtcaatcaac gatttattta gaagggaaca acaaaaaggt tatcgtagat
     1801 ccgtttattt caaataaccc taagtgtgat ttgaatattg aaacagtgca ggtagactat
     1861 atcgttttaa cgcacggtca ttttgatcat tttggtgatg ttgtagaact agcaaagaaa
     1921 acagaagcaa cagtaattgg aagtgcagaa atggctgact atctttcttc atatcacggt
     1981 gttgaaaatg ttcatggtat gaatattggt ggtaaagcaa actttgattt cggaagcgtt
     2041 aaatttgttc aagcatttca tagttcaagt tttacacatg aaaatggtat tccagtttat
     2101 ttaggtatgc caatgggtat cgtgtttgaa gttgaaggaa aaacaattta tcatactggt
     2161 gatacaggtc tgtttagtga catgtcactt attgcgaaac gtcatccagt tgatgtatgt
     2221 tttgtaccaa ttggagataa ttttacgatg ggtattgatg atgcaagtta tgctattaat
     2281 gaatttatta aaccaaaaat ttctgtaccg attcattatg atacattccc attaatcgaa
     2341 caagatccac aacaatttaa agatgctgtt aatgtagggg acgtgcaaat tttaaaacca
     2401 ggtgaatctg ttcagtttta aatagaatat agattttaca ctaaaaaaca cctcgctaat
     2461 ctatgtggaa gttatcgtct gcatattttt aatgggtgtt tttatattct actgattcag
     2521 ttgcgacgtg gacgggggag acatcagtgg ttctttatca tttctgacct actctcgtaa
     2581 ttttagtttt atacaattag acattgatta taaatttact acgatataaa aaatccagaa
     2641 ccacagtcat aatcatttct tgtgtttact cagtcgtttg agactgcatc atgattgaaa
     2701 gattatttgt gattctggat aaaaatttta tttaacgatt aatacaggga tagtcgcacg
     2761 ttttgctact ttgtggctga cacttccaag tacaaatttc tttttgtctt ctgctttacg
     2821 gttacttaaa acaacaattt cgtatttacc gctattagca tgttttaata gttcttcttt
     2881 tgcattacca cgaacaatta tttggtcata atcaatacca taatcttcta aagctttgcg
     2941 tgttttttcc aatcgcttgc ttcgttcttc agtaagtttg tttaaatgaa cacctgcttt
     3001 aattgatgct tgagcatctt gttcgctgat tgcgtttaaa actgttacga ctgtaccttc
     3061 gccagctaat ttagacactt cttttagtgc tttttcattt tttaactgag tgtctacacc
     3121 aagtaatata tttttataca ttagtacatc cctcctcact tacattctaa taaataataa
     3181 tggatttttc catttaaaat taaagatttt gtgatctttg tgtttgttgt atattttcaa
     3241 aaaataaaat aggtgaaacg atcattattt catttcactt caatgctttt tatccaaaat
     3301 aaagttataa tgtagttaat actgttcgaa aagaagaggt aaagaatgac aaaacatgaa
     3361 caaattttag attatattga atcgttatct ataggctcta aaatttcggt gcgtaaaatc
     3421 gctaaatttt taaacgtatc tgaagggaca gcatatcgtg caattaaaga tgctgataaa
     3481 atgggcatgg tcgcaacgat agatcgtgtt ggtacggttc gaatcgaaaa acgtaatcgt
     3541 aatgaaattg aacatttaac ttttaatgaa attgtcaata ttattgatgg ccaagtgtta
     3601 ggtggcaaca aaggtatcac taagatggta tctaaatttg caattggagc aatggaactc
     3661 aaagatattt taaagtatat tgggccaaag acattattga ttgtcggaaa tcgcgaagat
     3721 gtgcagattg aagctttaaa aagaggcacg gctatattaa ttactggtgg atttaagcca
     3781 tctaataaag ttattgattt tgcgaatgaa catgatttac ctgttctatc atcaagttat
     3841 gatacattcc tagttgcaaa tattatcaac aaagcacttt ttaatcagaa aatacgaaaa
     3901 gatatcttaa tagttcaaga tataatgacg ccattagatg atttatctgt gctgtttgat
     3961 acgatgaaaa tagcagatta taagcgtatg gcgaatcaaa ctggccatac aagatttccg
     4021 gtagtcaatg agtcttataa attagtaggt attgtaacaa gtcgagagat gattaatacg
     4081 aaggacgacg acgaaataga taaagtgatg acgcgaaatc ctatttatgt aaatgctatg
     4141 agtacagtag caagttgtgc gcatatgatg atttgggaag gtattgaatt gattcctgtc
     4201 gtttcgagta ataaaaagac agtgggtgtg attaatagac aagatgtttt gaagtctatg
     4261 caacttttag gaagacaacc tcaaatgggt gaaacaatta acgatcaaat cgccaaatat
     4321 ataacaatga atcaagatgg aattacagta gaagtttcac cactgttaat caatcactac
     4381 ggaacagtaa gtaaagcagc atttgtaagt attatagaag aaacaataca atatgaaatg
     4441 cgtaaattta aaaaaggtaa tgttatgata gagaacttaa atatcgtgta tattaaaaca
     4501 gtacctattg aatcacatat aacagtgcgc tttggcatac tagacgtcgg aagaaacttc
     4561 gccaaaatag aagtgaacat gcatagtcaa aacgacaaag tagcgagcgc tctcgtgata
     4621 tgccaaatgt ttgacgaagt ataggaactt agcagagaaa ataggcatac gatagatttc
     4681 acacaaatgt tggagcccaa cttgcattgt ctgtagaatt tctttttgaa attctctgtg
     4741 ttggggcccc accccaactt gcacattata gtaagctgac attacgtcac cttctgtgtt
     4801 ggggcccctg aactaattag tatgaaaaaa tatatatcta aataattaaa tcgggaaaat
     4861 agtttaatat ggtagaacaa tttttataat ttagatattt tgatatagac taatgcttaa
     4921 attaagcaat ttttctaaaa aagggagaat gaaaatgatt agtacgatga atgaaattat
     4981 gaaatgtatc gaagacaacg atacaattat tatacacaga catgttagac cagatcctga
     5041 tgcatatggt tcacaacttg gtttaaaata ctatattcag cagaaatttc cgcaaaagca
     5101 agtttttgca g
//
LOCUS       DAMZPP010000165         5022 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMZPP010000165 DAMZPP010000000
VERSION     DAMZPP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.166"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..707
                     /locus_tag="POH73_002497"
     CDS             <1..707
                     /locus_tag="POH73_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDI6888408.1"
                     /translation="YKDKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDIKS
                     VFNSQMTQNSDFNEEKKVSTKVYLDQIHQRLFLEQSLITERIKKYFKNQLTEQIAPIV
                     QQLADLHVIVNPQFNFESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHES
                     ICQSTLGLLQPQMGLLRQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTL
                     INQLKDKHQQLKTILY"
     gene            727..1605
                     /locus_tag="POH73_002498"
     CDS             727..1605
                     /locus_tag="POH73_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HDI6888409.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGVPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            2080..3198
                     /gene="ald"
                     /locus_tag="POH73_002499"
     CDS             2080..3198
                     /gene="ald"
                     /locus_tag="POH73_002499"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDI6888410.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLATSHDLDYKEILNVIE"
     gene            3293..4333
                     /gene="tdcB"
                     /locus_tag="POH73_002500"
     CDS             3293..4333
                     /gene="tdcB"
                     /locus_tag="POH73_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDI6888411.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            4364..>5022
                     /locus_tag="POH73_002501"
     CDS             4364..>5022
                     /locus_tag="POH73_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6888412.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AG"
BASE COUNT         1892 a          709 c          917 g         1504 t
ORIGIN      
        1 attataaaga taaacagcgt ttacaacatc aattaattga tgcgacgcta caacatacag
       61 acaacgaagt tgaagaacaa gtttatcatt taaatgcccg tttaaaacta caactactag
      121 acgatattaa atcagtgttt aattctcaaa tgacgcaaaa tagtgatttt aatgaagaaa
      181 agaaagtgtc tacgaaagta tacttagatc aaattcatca acgattgttt ttagagcaat
      241 ctttaattac agaacgtata aaaaaatact ttaaaaatca actcactgag caaattgcac
      301 caatcgttca acaattagca gatttacatg tcattgttaa tcctcagttt aactttgaat
      361 cagctaatat agagcaacca ttattgcaca ttgatttcaa cgatatgcta aatgcattgc
      421 ctaaacaatt aacaaaacgt aaaattttga atccaaatgg gcaaagagat atacatgaat
      481 caatttgtca aagtacgtta ggattattac aaccacaaat gggattattg aggcaacagc
      541 ttgaattata tgtaaagcaa atggctgtag aagctgaatc gcaatttgaa agttttgaag
      601 ctaatattca aacgcaaata aacgatttat tagcatttga tttagataca acacttatca
      661 atcaattgaa agataaacat caacaactga aaactatttt atattaagaa agaaggaacg
      721 ttttaaatgc caaataaaat attacttgta gatggtatgg cgctattatt tagacatttc
      781 tacgctacaa gtcttcataa acaatttatg tacaattcac aaggtgtacc tacaaatgga
      841 atacaaggat ttgtgcgtca tatcttttcg gcaatacatg aaatacgccc tacacatgta
      901 gctgtatgtt gggatatggg gcaatcaact tttaggaatg atatgtttga cggttataag
      961 caaaatcgtt ctgcaccacc agaagaattg ataccacaat ttgattatgt taaagaaatt
     1021 tcagagcaat ttggctttgt aaatattggc gttaaaaact atgaagcgga tgatgttata
     1081 ggtacattag cacaacaata ttcaactgat aacgatgtct atattattac gggcgacaaa
     1141 gatttactgc aatgtattaa tgacaatgtt gaagtctggc taattaaaaa aggttttaac
     1201 atttataata gatatacatt acatcgtttt aacgaagaat atgctcttga accacaacaa
     1261 ctaatcgata ttaaagcgtt tatgggtgat acagcagatg gatatgcagg tgttaaaggt
     1321 attggcgaga aaacggctat taagttaatt cagcaatatc aaagcgttga aaatgtggtt
     1381 gaaaacattg atgcattatc agctggtcaa cgtaataaga taaatgacaa tttagatgaa
     1441 ctatatttat cgaagcgact tgctgaaatt cacacacaag taccaattga tagtgaagcg
     1501 ctttttgaga aaatgtcatt tgcaacaacg cttaatcata ttttatcaat ttgtaacgaa
     1561 catgaattac atgtttcagg aaaatatatt tcatcccatt tttaaaaagt ataagatttt
     1621 atgaaaaatt aaaccaatta agtataaaaa gactatttct atcgatacaa ggtagaaata
     1681 gtctttttgc gtatttgaat atgtcagagt gaataaataa gccgaatttt gatagataca
     1741 tatttgatta atcaatagag tataaagaat tatatttcat ccaaataaat taccatacac
     1801 tacaaaatag tacagttaca actttattaa tctaaaaaac atatttgatt aattattttt
     1861 tgagtcaatg tattaataca actataaatc aaatggagta taagaaataa atatagtggt
     1921 ataattgcac ataatattta agaataataa taatatatta aatatttatt aacaaattat
     1981 agataaaata tgaataatta attaatggta tttacatatt cataaccaaa ggataaaata
     2041 attgtgaaat tattcacaaa tgaaagagga gtgttgcaaa tgttagttgc agtagtcaaa
     2101 gaattaaaac aaggtgaagg acgtgtagct tgcacacccg aaaatgtgcg taagttaacg
     2161 gatgcaggac ataaagtaat tgttgaaaaa aatgctggca ttggttcagg attttctaac
     2221 gatatgtatg aaaaagaagg cgctaagatc gtaactcacg aacaagcatg ggaagctgat
     2281 cttgttatca aagtaaaaga acctcatgaa agcgaatatc aatatttcaa aaagaatcaa
     2341 attatctggg gatttttaca tctagcatct tcaaaagaaa tagtagaaaa aatgcaagaa
     2401 gttggtgtaa ctgcgattag tggtgaaacc attataaaaa atggaaaagc agaattatta
     2461 gcgccaatga gtgctatagc aggtcaacgc tcagcaatta tgggagctta ctactctgaa
     2521 gcacaacatg gtggtcaagg tactttagtg actggtgtac atgaaaatgt ggatatacct
     2581 ggtagtacat atgtgatttt cggtggtggc gtagcagcaa caaatgcagc aaatgttgcc
     2641 ttgggactaa atgctaaagt aatcattatc gagttaaacg atgaccgcat taaatatctt
     2701 gaagatatgt atgcagaaaa agatgtcaca gtagtcaaat caacaccaga aaatttagca
     2761 gaacaaatta aaaaagcaga tgtatttatt tctacaattt taattcctgg tgcgaaaccg
     2821 ccaaaattgg ttactcgtga gatggttaaa tcaatgaaaa aaggttcagt attaatcgat
     2881 atagctattg accaaggtgg aactattgaa acaattagac caactacaat ttctgatccg
     2941 gtgtatgaag aagaaggtgt gattcattat ggtgtaccaa atcaaccagg agcagtccca
     3001 agaacttcaa caatggcatt agcacaagga aatattgatt atatattaga aatttgtgac
     3061 aaaggcctag aacaagcaat taaagataat gaagccttaa gtactggagt aaacatttac
     3121 caaggacaag tgacaaacca aggattagct acatcacatg acctagatta taaagaaata
     3181 ttaaatgtta tcgaatagat agtgatttaa atgaaattga gtgaaatgaa tattttaaat
     3241 atagcattat agtttggact aaaaatttac aaaacggaag gatgtaatta ttatgacaac
     3301 caacacagtt acattacaaa cagcacatat tgtaagttta ggagatatcg aagaagctaa
     3361 agcaagcatt aaaccattta ttcgtcgaac acctctaatt aaatcaatgt atttaagcca
     3421 aagtataact aaagggaatg tatttctaaa attagaaaat atgcaattca caggatcttt
     3481 taaatttaga ggcgctagca ataaaattaa tcacttaacg gatgaacaaa aagaaaaagg
     3541 cattatcgca gcatctgctg ggaaccatgc tcaaggtgtt gctttaacag ctaaattatt
     3601 aggcattgat gcaactatcg taatgcctga aacagcacca caagcgaaac aacaagcaac
     3661 gaaaggctat ggggcaaagg ttattttaaa aggtaaaaac tttaacgaaa ctagacttta
     3721 tatggaagaa ttagcgaaag aaaatggcat gacaatcgtt catccatatg acgataagtt
     3781 tgtaatggca ggccaaggaa caattggttt agaaatttta gatgatattt ggaatgtgaa
     3841 tacagtcatc gtaccagttg gcggtggagg attaattgca ggtattgcca ccgcattaaa
     3901 atcatttaac ccttcaattc atattatcgg tgttcaatct gagaatgttc atggtatggc
     3961 tgagtctttc tataagagag atttaactga acatcgagtg gatagcacaa tagcagatgg
     4021 ttgtgatgta aaagttcctg gtgaacaaac atatgaagta gttaaacatt tagtagatga
     4081 atttattctt gttactgaag aagaaattga acatgctatg aaagatttaa tgcagcgtgc
     4141 caaaattatt actgaaggtg caggcgcatt accaacagct gcaattttaa gtggaaaaat
     4201 aaacaataaa tggcttgaag ataaaaatgt tgttgcttta gtttcaggcg ggaatgttga
     4261 cttaactaga gtttcaggtg tcattgaaca tggactgaat attgcagata caagcaaggg
     4321 tgtggtaggt taaaacattt aatctgaaaa atgaggtgta attatgtcaa atggtaaaga
     4381 attacaaaaa aatataggtt tcttctcagc gtttgctatt gttatgggga cagttattgg
     4441 ttcaggagta ttctttaaaa tatcaaacgt aacagaagta acaggaacag caggaatggc
     4501 cttgtttgta tggttcctag gcggcatcat taccatttgt gcggggttaa cagcagcaga
     4561 acttgctgct gcaatccctg aaacaggtgg cttaacaaag tatatagaat atacatacgg
     4621 tgatttctgg ggcttcctat caggctgggc acaatcattt atttattttc cggcaaacgt
     4681 agcagcattg tctatcgtat ttgcgacaca gttaattaat ttattccatt tatctatagg
     4741 ttcattaata ccaatagcaa tcgcatctgc gttatctatt gtgttgataa atttcctagg
     4801 ttcaaaagca ggcggaattt tacaatcagt taccttagtt attaaattaa ttccaattat
     4861 tgttatcgtt atctttggta ttttccaatc aggagacatc actttttcat taattccaac
     4921 tacaggtaat tcaggaaatg gtttctttac agcaattggt agtggtttat tagcaactat
     4981 gtttgcatat gatggttgga ttcatgtagg aaatgttgcg gg
//
LOCUS       DAMZPP010000166         4947 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMZPP010000166 DAMZPP010000000
VERSION     DAMZPP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.167"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..566
                     /locus_tag="POH73_002502"
     CDS             <1..566
                     /locus_tag="POH73_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001803819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDI6888413.1"
                     /translation="DSSLRSEDKGDDMSTLVGTVLSGFEYRAHKQELDSLTEVLKEYK
                     SKYKYTGYTENAIMKTQNSGFRNEYYYLTAIPYTLDEYKKYFQPLIKEDDKTFSDGMK
                     NSKKQLKDKSRPYVVTTLFSTKDNYTKDNTIDEMIDFSEVLKKKKNIPHDLNVSLQIT
                     DKYINSARPSYSKKDSIEVGVFNHEKN"
     gene            553..2594
                     /locus_tag="POH73_002503"
                     /pseudo
     CDS             553..2594
                     /locus_tag="POH73_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2637..3842)
                     /locus_tag="POH73_002504"
     CDS             complement(2637..3842)
                     /locus_tag="POH73_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI6888414.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGVIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDREL
                     TQKLENIPS"
     gene            3968..4582
                     /locus_tag="POH73_002505"
     CDS             3968..4582
                     /locus_tag="POH73_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDI6888415.1"
                     /translation="MTQFLGALLLTGVLGYIPYKYLTMIGLVSEKNKVINTPVLLIFS
                     IETCLIWFYSFIIFNNVDLKNLNLIQLLTGLKANILFLFIFVLTVFVFNPLIVKFIIW
                     LINITRKFMKLDCISLLDKRDKLFNNNGKPVFIVIKDFENRIIEEGELKTYNSAGSDF
                     DLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLMNEY"
     gene            complement(4579..4725)
                     /locus_tag="POH73_002506"
     CDS             complement(4579..4725)
                     /locus_tag="POH73_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888416.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(4762..4944)
                     /locus_tag="POH73_002507"
     CDS             complement(4762..4944)
                     /locus_tag="POH73_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888417.1"
                     /translation="MKITNCKIKKETIVYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLTKEDSL"
BASE COUNT         1768 a          609 c          786 g         1784 t
ORIGIN      
        1 gtgatagctc attaagaagt gaggataaag gcgatgatat gagtacttta gtaggtacag
       61 tgttgagcgg ctttgaatat agagctcata agcaagagtt ggatagttta acagaagttt
      121 tgaaagaata taaaagtaaa tataaatata ctggttatac agaaaatgca attatgaaaa
      181 cgcagaatag tggttttaga aatgaatatt attatttgac tgcaattcca tatactttag
      241 atgaatataa aaagtatttt caacccttaa tcaaagagga tgataagact tttagtgatg
      301 gtatgaaaaa ctcaaaaaaa caacttaaag ataaatctcg tccatatgtt gttacaactt
      361 tatttagcac taaagacaat tacactaaag ataatacaat agacgagatg attgattttt
      421 ctgaagtact aaaaaagaaa aagaatattc ctcatgattt gaatgtttct cttcaaatta
      481 ctgataaata tataaattca gcaagaccta gttacagtaa gaaagattca atagaagtag
      541 gagtatttaa tcatgaaaaa aattaatgat agagatttag cagaattaag tggatactgg
      601 gcatatcaag atttggacaa aagatatgta attagagtaa ataataataa atatagacta
      661 attgaagatt attcagacaa tcctaattct aaaagttttg gtggtacaga tgcaaaagtt
      721 tttgaattgg taaatagcaa taatgaatta acaggacaac aagctattat cttccaaggt
      781 acaaataata ataagagtat cggaagcaac aatccttttc gtgggaaggt tgctgatgat
      841 tggattgaaa atataaagtt aatgaatgat aaaaataagt caacttcttt attagaacaa
      901 aataatgctt atattaaatc gtatgaacag aaactaaaag atgcagatac acttaaaagc
      961 tctgattttt taaggaaata taagaaaaat ccgtctacgt ataaaaacaa aactattaag
     1021 tctgatggag gcaattctca agggggagca agtgctaacc accaagggat gattaacccg
     1081 aataaaaata tagtttctac aaaccctgcc atgttaccaa agtcaatatg ggaaaatttc
     1141 aaatctcaaa actttgaaaa tatgattaat tatcatagta agtttgatat tttatcttgg
     1201 ctgcaggatc catttgccac tccgacacta ggaaaacgag tgaatttaga aactggtgtg
     1261 ccaacgatgg atggattagt aaatagtcat ttaggttata aaagaaaatt aaatccaaga
     1321 tataatactt ataaggattt agcagtttat aaaattaaat cagttaaaga tactatgatt
     1381 aaaaatggta aaaaagtcaa aaaaactatt gaaatcgata taaatatgga tgatagaatt
     1441 ccaattaatg tgtggacagg agattcaata gcgagaacgg gtaaagggac tcctataaaa
     1501 ttaaatctag aaaatcttag tgcattaagt aatttggtaa ctggtgaaac aagtaatatg
     1561 ttaaccgatt gtgtaaatta tttgaatgag agctataata tttcacaggt tgaaaatgct
     1621 aattttggag aaagaaaaca taaacttaaa caagatttta aaaagattgt cgaagttgat
     1681 atccttgaag gaatgagtag agaattaact tctttgaaaa aggatgcatt tagcgcaatt
     1741 gataatgtca aaaaaaatct tgttggaatt gctttcatat tttcatcagt tgcagtcctt
     1801 atttttgaat taaatactat tgaagaaatg cttgaagatt tagataaaaa attacaacgt
     1861 ggcatagaaa gtttacatga ttcattggat aacgtaatta aagaaatgtt taaaaattta
     1921 gatcatgatt ttgaagatgg cgtgacagag gaaatgatga agcatttaaa tgttgtgaat
     1981 aataatataa attttgtaaa aaacaaaatg atgtatatgg agagcagata accgatatta
     2041 aaaatataat gtctcatcaa gatgcaacgg taatggatgg aaacttatct attaattata
     2101 gcggtgaaca tatgatttcg ggtcaagtac aatcttctaa ttatttaact agaaaaatga
     2161 caattttaaa aaaccatatt gataatgcag ttaacaaaat tagtgattat gttcaagaag
     2221 cttacaataa ttattttgaa ccagtattaa actacattct tgaagttata cattcggtag
     2281 aaacagctgt gagacatatt tatgaaataa gcagtttaat atacaaagta acaatgaaaa
     2341 taaagataaa ggctatggga ataaatgtcg acgaaattga taaaaacctt gaagttttaa
     2401 aaagtaaact taaaaattta gatgaatttt taaataactt aaaaacagct tcaccaattt
     2461 tagaaaatca cttagatgac attgttagaa atatgaagcc attaattgtt aatcaaattt
     2521 ttgaaccatc tcattatgat gatatgttta tttcaagaaa agctttaacg ccagtgttct
     2581 caagcgtttt ataaagcttg taaaaaatat aagggcaaaa aaagggcaga tttaagctaa
     2641 cttggaatgt tttcgagttt ttgagttagt tctctatcca ttttttcagt tacatgagta
     2701 tatatgcgaa tggttgtttt ttcatctaca tgtcctaccc ttttcataat tgcttttaaa
     2761 gaaacattca tttctactaa taaagttatg tgtgtatgtc taaatgtgtg cgtggtaact
     2821 ttcttattca tatttaaagc ttttgtagtt ttcttaagca caccggcgat ttgattatta
     2881 cataaaggat tccctttttt tgttgtgaat atgaaccctc tgtcaacata gctcgaattc
     2941 catcttttca acattttgtt ttccagtatt atctttttaa aaatttctac ggttctagaa
     3001 ttgatgctga tacttctttt tgaacttata gtctttgtag tgtctttgta tccgaatcct
     3061 tcctcgtatt taatgcggtg aattgtacct gttatattaa tagttttgtt taataaatct
     3121 atatcttttt cctgcagtgc ttgtagttct cctatgcgca taccagttaa agcctgtact
     3181 tctaagatgc tggcaattaa aatgcgattt cgcttgtgta acttattatc atttagtata
     3241 tgatcacgta tctgtaggac ttggttcatt tctaaatagt tgtacatttt agattcatct
     3301 ttttcgatat cctctattgt ttttcttctt ttaggaattt tgacattagt taacaaatat
     3361 tcatttggat aattgtaaaa tttaactgca tatttaataa ctcctttcat atctccgagt
     3421 tgacgggtta cttgattttg agaatagata tctgataatt tattaataaa tatctgcata
     3481 tattttgtat ctagtttgtt taaaagcaag ttctcagagc tgtatcgttt aatgtttcta
     3541 attcttattt ttatattatt aagagtagtc aactttgaac ctgatgtttt tatatgatat
     3601 tcaagccatt catctaatag cgcgtgaaaa gtcaaagttt ttaattcgct tgacgacttg
     3661 ttgttcagtt tttcttttat tttttcttct aaacgaaaca ttgcttcttt ttgagattgt
     3721 tttgtattct tgttcaacac aacacttacg cgcttccatt tatctgtgta tggatctttg
     3781 tacttctcgt aatatctgta tttagtttcg ttatttttgt ttttaaattt ttcaatccac
     3841 atgtttatac ctcctgtagg aacgtacgtt ctgtaaattt gtaaaaaata ataagggtag
     3901 gtgggctacc caaaatttag tactaggtac taaatatgtt ataataaaat aaaaagtagg
     3961 tgataagatg actcaatttc taggggcgct tcttcttaca ggagttttag gttacatacc
     4021 atataaatat ctaacaatga taggtttagt tagtgaaaaa aacaaggtta tcaatactcc
     4081 tgtattattg attttttcta ttgaaacatg tttgatatgg ttttatagtt ttataatttt
     4141 taataatgtt gatttaaaaa atttgaattt aattcagttg cttacaggtc taaaagcaaa
     4201 tattttgttt ctatttattt ttgttttaac agtgtttgta tttaatcctt taattgttaa
     4261 atttattatc tggttaatta atataaccag aaagtttatg aaattggatt gtataagctt
     4321 attagacaaa agagacaagt tgtttaataa caacggtaaa ccagtattta tagttataaa
     4381 agactttgaa aacagaatca ttgaagaggg tgaacttaaa acctataatt cagctggtag
     4441 cgatttcgat ttactagaag ttgagcgaca agatttcaaa gtatctgatt taccgtcaaa
     4501 cgatgaattg tatattaaac atacgcttgt agaccttaaa caacaaatta aattggattt
     4561 atatttaatg aatgaatact aatctttttt cttagctttt tctgataaag tgctttttaa
     4621 tttttcgctg gcgcctgact tttcaaaact tttgtttaat gggttactac gagtagtttc
     4681 ttgttttttg tttttatcta ccataaaatt ctcaccacca ttcaacgtct acactagtag
     4741 gcgttttttt atttagtaaa atcataatga atcttctttg gttaacttat ctccatctat
     4801 tttttgtgaa ataaattcca agtatttacg cgcattatgt gacgataaat ctttaggtaa
     4861 ctcataagtg aatggttgat taccactagt taaaacttca tatactatag tttctttttt
     4921 tattttgcaa ttagttattt tcattat
//
LOCUS       DAMZPP010000167         4884 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMZPP010000167 DAMZPP010000000
VERSION     DAMZPP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.168"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..497)
                     /gene="deoC"
                     /locus_tag="POH73_002508"
     CDS             complement(<1..497)
                     /gene="deoC"
                     /locus_tag="POH73_002508"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDI6888418.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSGVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTKAAGADFVKTSTGFAGGG
                     ATAEDV"
     gene            812..1522
                     /gene="deoD"
                     /locus_tag="POH73_002509"
     CDS             812..1522
                     /gene="deoD"
                     /locus_tag="POH73_002509"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDI6888419.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(1643..2086)
                     /locus_tag="POH73_002510"
     CDS             complement(1643..2086)
                     /locus_tag="POH73_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDI6888420.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(2287..2700)
                     /locus_tag="POH73_002511"
     CDS             complement(2287..2700)
                     /locus_tag="POH73_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HDI6888421.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFTTLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FADNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
     gene            complement(3149..4522)
                     /locus_tag="POH73_002512"
     CDS             complement(3149..4522)
                     /locus_tag="POH73_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HDI6888422.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRMSNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
BASE COUNT         1490 a          884 c          681 g         1829 t
ORIGIN      
        1 acgtcttctg cagtcgcacc gccacctgca aaacctgttg aagttttaac gaagtccgca
       61 ccagccgctt ttgttaattc actcgctttt acaatttcgt catggtccaa caataccgtc
      121 tcaataatca cttttactgt gtgacctttc gcagctttaa ccactgcttc aatgtcttgt
      181 tgtacatcat caaaacgccc atcttttaat gcgccgatgt tgatgaccat gtcaatttca
      241 tctgcaccat tttgaattgc atcttctgtt tcaaatgctt tcgttgcagt tgtcgacgca
      301 cctaatggga accctattac cgtacaaacg agcacacctg aatcagctag tcgctctgct
      361 gcatatttta catgcgttgg attcacacat acagatttaa aattgtatgc tttcgcttca
      421 tcgatgattt gatcgatttg cgtacgtgtt gactcaggct tcaataaagt gtgatcaatc
      481 aattttgcac tattcatttt ctatctcctc ctttatggtt gattataaaa atacggttgt
      541 aaattagttg attgagcgtc aggttcattt aaatatcagg ttagatgttc gctttttatg
      601 taaccgcata catatactat tacattaatt catttcccat aaacaaacaa tacaattgaa
      661 cgtgatatct tcattatgaa cgatgacttg acaacaagct aatcgggatt atatttttat
      721 aattctttaa ttctatagta caaaaattcg caaaaaaggg aaacaaatgt tatcttaaaa
      781 ttattaatga atattaagga gaagataaca aatgacaaaa ggtacaccac atattcaacc
      841 aaatggagta aaaattgcta aaacagtatt aatgcctggc gatccgctac gcgcaaaata
      901 tattgctgat aattttttag aaaatgttga acaatttaac gatgtacgta acatgtttgg
      961 ttacactggt acatataaag gtaaagaagt ttctgtaatg ggttctggca tgggtattcc
     1021 aagtattggt atttactcat atgagttata caacttcttt gatgtagata caatcattcg
     1081 tatcggttct tgtggcgcat tacaagaaaa tgttaactta tacgatgtta ttattgcaca
     1141 agctgcatca actaattcaa attatgtaga tcaatacaat attccaggtc atttcgcgcc
     1201 tatcgctgac ttcgagttag taactaaagc taaaaatgtc gctgaccaaa tcggtgctac
     1261 tacacacgta ggtaacgtat tatcttctga tacattttac aatgccgatc caacattcaa
     1321 tgatgcttgg aaaaaaatgg gtattttagg tatcgaaatg gaatcagctg gtttatattt
     1381 aaatgcgatt catgctggta aaaaagcact tggtattttc acagtaagtg atcatatttt
     1441 acgtgacgaa gctactacac ctgaagaacg tcaaaattca tttacacaaa tgatggaaat
     1501 cgctttagaa atcgcagagt aacttattta aattgagttt aattgctctt taacaatgcg
     1561 attaaactca aaaagccaac acattctggg cgtatcccca tttatgtgtt ggcttttatt
     1621 tatattatta cttatctgta gattagctta agtaagattt aaacatccaa ttatgtttat
     1681 ctactgatgt ttgcatacct ataaacatat cttctgatac atcatcgcct gcattaccag
     1741 caatttcgat tgcgttttct aattgttttg agatatttgt gaagtcttgt gataattctt
     1801 caaccatttg ttctgcagag taacctttcg cagcttcttt aacaattgat tgctctaagc
     1861 attcagttaa tgtacctaca gggtttcctc ctaccgctaa aattctttca gctaattcgt
     1921 ctacatattg gcttgcttca ttatataatt cttcaaattt aacgtgtaat gagaagaagt
     1981 taggcccttt cacataccag tggaaattat gtagctttgt ataagctact gtccagtttg
     2041 ctacttgttg attcaattct tttacaacat cttgttgatt actcatattt aatacactcc
     2101 ttaaaattgt ctacgtcttg cttgtttata ttaattatta tattataatg attctaatct
     2161 gtaaagtgtt ttttactctc ttttttataa ttattctaat ctatctgtta agtgttccgc
     2221 tttttaaata tctaaatttt aattcatcat ctagtatatc tattatcaat tagaaagtat
     2281 gttttatcaa tcagtaagtt ttactttcgc attatcatct aaaaagaacg atttttcata
     2341 atcatttggt tggtgccatg ttgttttcca cattctattt ctattatcag caaacattcg
     2401 ataaccgaaa ttacggactg gtttaggtac aagccataaa cctacgccca ataatttagt
     2461 tcgattaggt agtgctgtga ttagtttaat aatcgcctgc gattcaaaat atatacgatc
     2521 acctttctgt aaaatcacac tatttttatt tgccgcttct ggatgttgtt taaaaaattg
     2581 ttgtccaact tcacctttta acgtggtaaa ttgataattt cttggtaggt catgttgaat
     2641 taaccagatg acatagttat aacaatatat acagtttcca tcataatata cgattggcat
     2701 aatttattcg ctccttcaat agtacttata gtcatttttc ctaatttaaa aacatttaaa
     2761 caaggttgtc acttgataag tcctcgcgac acgaaattgc cataaaattt atttttcagt
     2821 tttgtattta ttgtataatg cattttttat ttactttaaa tttctcaatc tacagtgtaa
     2881 tcttatttct tattatcaac ttagcgttta gtcgtttgtc ttattcaaca taccactgga
     2941 aaactcaaat aaagttttaa ttcagtaaca taaattcatt tatattcata tactgtcttt
     3001 attatttatt aaaataaaaa cgagtaatct atcataagct cttaattagc tcttatggtt
     3061 gattacccgt cattttttct ttatacggtt attcaaatat gcaattttta atctttcctt
     3121 acttcttttc aaaaactacc atatattatt agtcatgttt gactttgact atacgtgtgt
     3181 attttaaaaa ctgctctgaa tatttctcct taatttcctc taaactatca tatgtaatac
     3241 ctacaatatg ccaattagga ttgaaataat agaaagaatc tttttgtcgt gaccatttaa
     3301 tcatattacc gtctttatta taacgtggta atgttacctc atagccttct aacacattaa
     3361 tatacgtttg gaaaatgtct gtatctatac gattaaaatt atcaataact aaataacttt
     3421 tatccttttt aggcattaat tgagtaataa atccttcacg atagtataat gcccctgttt
     3481 catttggtaa atacttgcca taaagcatat cttctgaaaa atcaggatga cctgttgtta
     3541 taacaggatt tgcattcgct ttttgcaata agtcattagc cgcttttaag ccttcctctt
     3601 ttcgatctac aacaagtaaa ataaatggct ttaacgcttc ttctttttca ctttcaagca
     3661 tatctggttt ttgaaccatt tcaaatggag atttcaatcc attattatcg ctcatttcaa
     3721 taattgcatc atactgtgct tgtgacatac ttgcaatagc ctgttttgca ttttcttgaa
     3781 ggaaatataa atttttcaat ttaggatgct tatttaatgt acttaatgta atcggcgtaa
     3841 tgtctttctc ataagacact tcaatcactg tactatttgt tctaccagga attggtggtt
     3901 tttcatgaat atgctttgat acttctccaa ttccaacgac agattgattt ttcgttcgat
     3961 tataaaaaat aatattgtcg ccttcttcta actgagtata aaaatgataa ccattacgtt
     4021 taataccgtt gtacgtgtgc gtataaatcg tatattggtt tccaggttca aattcttcag
     4081 tttcagctaa aaagaaataa cgtggtatct taatttcgcc tttaccaaga ccacttatta
     4141 aatcaaactc ttctgcagtg atttggttga acaatgtctc tttcatatta ctcatacgaa
     4201 attccaaagc ttcactacgc tttaaataat ctgctgttaa tggtttcaat tgttcattaa
     4261 aacgaaactg tacacgtatt ttattttgtg cacctgtttc aacactaata atttcaccac
     4321 atccaagtag tccagtatcc gtctgaactt gataaaagat gacttgatct cctactttag
     4381 cctttttaaa cgctctaaat ccttgagatg gattaaaatg tgcgcctgat tcaaataaag
     4441 ctgtttgtcc tactaacggt tcattatgat tccaacggtt atatccacaa ttcaaccaaa
     4501 aataattcgt ttctgctgtc atcttaatac tccttaacct gaataaattt tagaaacact
     4561 atgaattaca ttcttttaat gtttcttatg cagttgggcg cgtatacgat caactgtatc
     4621 ccctttgttc actgcgattt taatcgcatt tcctataaca ttgtagcgcc taggacatta
     4681 atttacgtcc cagaccctta tcgttttcac ttctaagtaa gtcgaactat tttgctttac
     4741 aacaagtgcg actctatatg cagttggaca cacacatacg agcaactgta taccttttaa
     4801 tcagttttct atattttatt tattatatat gtcttaatga taaaaattgt tacaaacagt
     4861 ttaacatatt tagctaccta tttt
//
LOCUS       DAMZPP010000168         4849 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMZPP010000168 DAMZPP010000000
VERSION     DAMZPP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.169"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..706)
                     /locus_tag="POH73_002513"
     CDS             complement(<1..706)
                     /locus_tag="POH73_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6888423.1"
                     /translation="MQNKSKSPFKIALSKFIHNKIAMLSVIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGI"
     gene            complement(718..1368)
                     /locus_tag="POH73_002514"
     CDS             complement(718..1368)
                     /locus_tag="POH73_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002486553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6888424.1"
                     /translation="MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLGNLISDITYGFIDPRIRSN"
     gene            complement(1361..2341)
                     /locus_tag="POH73_002515"
     CDS             complement(1361..2341)
                     /locus_tag="POH73_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDI6888425.1"
                     /translation="MENILEVNQVKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKLNKNRIILKGELPS
                     PSNPPQGCPFHTRCPIAQDMCKKSMPELKDIGNEHQVACFYVDKVGDLND"
     gene            complement(2344..3330)
                     /locus_tag="POH73_002516"
     CDS             complement(2344..3330)
                     /locus_tag="POH73_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6888426.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLLDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANNLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYQNEYKEI"
     gene            complement(3381..>4849)
                     /locus_tag="POH73_002517"
     CDS             complement(3381..>4849)
                     /locus_tag="POH73_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6888427.1"
                     /translation="DKKIHPKAYIASWKDIEPAKKIEFKIKKGIKWHDGNELKIDDWI
                     YSIEVLANKDYEGAYYPSVENIQGAKDYHEGKADHINGLKKIDDYTMQVIFDKKQENY
                     LTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYPIGIGPYKVKKIVPGEAVQLVKFDDY
                     WQGKPALDKINLKVIDQAQIIKAMEKGDIDVTNDATGAMAKDAKSSNAGLKVLSAPSL
                     DYGLIGFVSHDYDKKANKTGKVRPKYEDKELRKAMLYAIDREKWIKAFFNGYASEINS
                     FVPSMHWIAADPKELNDYKYDPEKAKKILDKLGYKDRDGDGFREDPKGNKFEINFKHY
                     SGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFGKYNEDLANASKDMEVYFRSWAGGTD
                     PDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDFDKVGIDEKKRKDIYVKWQKYMNDEL
                     PGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYNLTKEA"
BASE COUNT         1524 a          876 c          620 g         1829 t
ORIGIN      
        1 taattccgaa tcctaaaaag ctcaatcctg attccactac aatcataccg gcaaataaca
       61 atgttgcttg tacgataact acacttaata tattcggcaa aagatgtttc aaaataattt
      121 tatatgtggg tgtaccaatt gattttgctg ccaaaaagta ttcattttct ttttcttgaa
      181 gtactttacc acgaacaagt cttgcaatac ctccccaact taatacgata ataactagaa
      241 caagaattat ggcagatcca taaggatttt taattttatc tccaagtgca gcatttaata
      301 caattgcaaa tattaaaaat ggaaataaca taacaaattc ggttattctc attaataatg
      361 tatcaacaat acctccaaaa taaccagaaa tcactccaac tgtaattcca atcaaaagca
      421 ttcctattgt agatgtaatt ccaatggata atgaaatacg acctgcatat aacaaacgac
      481 taaagttatc cctaccacca gagtctgtac caagaatgtt ttgtgctgtc atttcacctt
      541 ttatatttaa taaatcttgt tggttcactg gaaaaggagc tattaatggc gctataattg
      601 atacaatagt tatgattaat aaaaaaataa ccgataacat tgcaatttta ttatgaataa
      661 atttagacaa tgcaatttta aaaggcgatt ttgacttatt ttgcattcta atccccccta
      721 gttacttcta attcttggat ctataaatcc ataagtaata tcagaaatca aattacccag
      781 taaacctaaa gtgaatgcag gtaaaaatgt gtgatatatt ttactcatat aatattcaaa
      841 agtaccttct ttaaggttaa tatcaacaga accttgaaac gggaaccatt gtaattcaaa
      901 agaaaaaata aaaattgcaa atactcccgc aataaaagat ggaatggcta acatcaaata
      961 attaaatatt tgcacagtat aatccgtcaa actgtaagaa tatctacctg acgttattcc
     1021 taaagcaaat gagataatat aagtaataat taatgacata gcaccgagta atattgtatt
     1081 tggaattctt tcctcaataa catcaattac cggcctttta tatttgattg actcccctaa
     1141 atcaccgtgt ataacatgat tgacccatct aatgtattga tgtggtattg agtcgttgag
     1201 acctaatttt tccctttgtg catttattgc ttcttgtttt attttaccgt tttgtagatc
     1261 tgaaaatgga tctcctggtt gtatgattgc taatgaaaat acaactattg aaattaaaat
     1321 tagtaacggg atcattaacc ctaatctttt aagtattaat ttaatcattt aaatcaccta
     1381 ctttatctac gtagaaacaa gcgacttgat gttcgttacc aatgtccttt aattctggca
     1441 tacttttttt gcacatatct tgagcaattg gacatctagt atggaaagga caaccttgtg
     1501 gcggattact tggcgaaggt aattctccct ttaatataat tctattttta tttagcttat
     1561 caatttgtgg tattgatgaa atcaacgctt ttgtataagg atgtttggga ttttcataaa
     1621 tttctttatc agatgcgatt tcaactatat gacctaaata cataactcca atgacatcac
     1681 ttatatgttt tactacactt aaatcatgtg cgataaataa atagcttaag ttaaattgtt
     1741 cttgtaaatc ttttaataaa ttcagtactt gagattgaac agatacatct aatgcactta
     1801 caggctcatc agcaacaatt aaactcggac gcaaagccaa tgctcttgca attcccactc
     1861 tttgtctctg tccacctgaa aattcatgtg catatttata atatgcatct tcacttagac
     1921 caacacattt taataaatat agaacttctt tttttatttc ttcttttggc aattttttat
     1981 aatttaaaat aggttctgaa atgatatctc caaccatttg catcggattc aatgatgcat
     2041 acggatcttg aaatatcatc tgatattgtt gtcgtgattt tctcagtttt ttaccttgta
     2101 atcttgttat atcttcacca ttaacaatta ttgagcctga agttgcatct tcaagtctga
     2161 taatcacttt acctaacgtt gacttaccac aacctgattc gcctaccaat ccaaaagttt
     2221 gtcctttttt tattgaaaac gatacgtcat caacagcttt aacgtactga gttttttgta
     2281 ataatccagt tttaattttg tagtattttt ttacttgatt tacttctaaa atattttcca
     2341 ttcttatatc tccttatatt cattttggta taaatgacac cgtacaatga cgtcttcaca
     2401 aacatgcatt ttaggagatt gattattaca aatatccagt tttttgttac atctatttgc
     2461 aaatctgcac ttattaacgt gaaatgcttg gattgatggc acaattcctt ctattgtttc
     2521 aagtcgtttc tgctcttctt taagttttgg aattgtagat aataattttt gggtataagg
     2581 atgtttcgga ttgtgtagta tttcttttat gcctccaaat tctacaattt gacctgcata
     2641 cataaccaaa actttatcgc aaaattcagc aaccacactc aaatcatgtg taatcatcat
     2701 aattgccatt tgcgtttcct tttgcaattc ttttaataag tctaatattt gcgcttgaat
     2761 cgtgacatcc aaagcagttg taggttcatc agcaattaat aatttgggag aacatgaaat
     2821 ggccattgca atcattactc tttgcctcat accaccagat aattgatggg gatagctatt
     2881 taatatttca tcttgtcttg caattccaac tttttttagt aaatcttttg ctaagttatt
     2941 tgcttctttt ttagaaattt ttttatgtga ttttatagat tccacaagtt gattttttat
     3001 agtaaatacc ggatttaaag cagtcatagg ttcttgaaaa atcatagcaa tatcatttcc
     3061 tcgaatatct agcaattgct tctcattgag cgtatcgatt cgtttaccat caaatataac
     3121 ttctccatta cttagtttag atatcttttc tggtaataat ttaataattg acttacttaa
     3181 tatggactta ccgcaaccag attcccctac tatacccaaa acttctcctt tagataattc
     3241 aaaagaaata ttttcaacag ttgctaacca ttcattattt atttttaatg atgtttctaa
     3301 atctttaacc tctaacaata cattattcat aacatccccc catataagta aacctcgaca
     3361 aaatcgtcga ggttacatta ctaagcttct ttagttaaat tatataaact ttgatcagtt
     3421 ccaatttcaa tgtctaagtt tcgtacttta tcattaacta tagttatcga tttaccttgg
     3481 aacattggta atccaggtaa ctcatcattc atatattttt gccatttaac ataaatatct
     3541 ttacgtttct tttcatcaat gcctacttta tcgaagtcta atgcatcatc taaatattga
     3601 tctgattttg gtaaaactgt tctcatttca ttttgaggtc tatcagtgtg gtataaatct
     3661 gatgggtctg gatctgtacc acctgcccat gatctgaagt acacttccat atcttttgat
     3721 gcatttgcta agtcttcatt atatttaccg aattctacta acttcacatt tgttttcaag
     3781 ccaacttttt cccagaaatc ttttatcgca gcagttcttg gttcaaaagt aggatttgaa
     3841 cctgaataat gtttaaagtt aatctcaaat ttattacctt taggatcttc tctaaatccg
     3901 tcaccatctc gatctttata acctaactta tctaagattt ttttagcttt ttcaggatca
     3961 tatttataat cgtttagttc cttaggatca gctgctatcc aatgcattga tggtacaaaa
     4021 ctatttattt cactagcgta accattgaaa aatgctttga tccatttttc tctatcaatt
     4081 gcataaagca ttgctttacg taattctttg tcttcatatt ttggtctcac tttaccagtt
     4141 ttattagctt ttttatcgta atcatgagat acgaatccta ttaaaccgta gtctaagctt
     4201 ggcgcagata ataccttgag accagcatta gatgacttag catcttttgc cattgcaccg
     4261 gtagcatcat tcgtaacatc aatatcgcct ttttccattg ccttaataat ttgagcttga
     4321 tcaataactt ttaaattgat tttatctaat gcaggcttac cttgccaata atcatcaaat
     4381 ttaacaagtt gaacagcctc acctgggacg attttcttaa ctttatatgg tccaatacca
     4441 ataggatatt ttcggatttt atctgatttc gctaaatctt taattggtac atctgataaa
     4501 tattttttac ttaataaagg tccagtaata aatcctgtta agtaattttc ttgcttttta
     4561 tcaaatataa cctgcattgt gtagtcatct attttcttca atccgttaat atgatcagct
     4621 tttccttcat gataatcttt tgcaccttgg atattttcta cacttggata ataagcacct
     4681 tcgtaatcct tgttagctaa gacttcaatt gaataaatcc aatcatcaat tttcaattca
     4741 ttaccatcat gccatttaat acctttttta attttaaatt ctattttctt agccggctcg
     4801 atatccttcc atgaagcaat atatgcttta ggatgtattt ttttatcaa
//
LOCUS       DAMZPP010000169         4848 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMZPP010000169 DAMZPP010000000
VERSION     DAMZPP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.170"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(111..806)
                     /gene="sceD"
                     /locus_tag="POH73_002518"
     CDS             complement(111..806)
                     /gene="sceD"
                     /locus_tag="POH73_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HDI6888428.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAQASEADLNKASLAQRAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGANGADMSAQAPATNNV
                     APSADQSNQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKASEGSSVNVNDHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     gene            complement(1195..1590)
                     /locus_tag="POH73_002519"
     CDS             complement(1195..1590)
                     /locus_tag="POH73_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6888429.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIERYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSILDIDSQNIDNHDLLEI"
     gene            1784..2224
                     /locus_tag="POH73_002520"
     CDS             1784..2224
                     /locus_tag="POH73_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HDI6888430.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            complement(2281..2721)
                     /gene="fabZ"
                     /locus_tag="POH73_002521"
     CDS             complement(2281..2721)
                     /gene="fabZ"
                     /locus_tag="POH73_002521"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDI6888431.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(2755..4020)
                     /gene="murA"
                     /locus_tag="POH73_002522"
     CDS             complement(2755..4020)
                     /gene="murA"
                     /locus_tag="POH73_002522"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDI6888432.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRGAIKEHMASLVYKLEEMGVE
                     LEYQEDGIRVRAEGDLQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            complement(4131..4364)
                     /locus_tag="POH73_002523"
     CDS             complement(4131..4364)
                     /locus_tag="POH73_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDI6888433.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
BASE COUNT         1544 a          846 c          749 g         1709 t
ORIGIN      
        1 aattatatat atttgcgaaa taaaaaagac cggaacatat cgagaattcc ccgatatatt
       61 ccaatctaaa agttacttat ataactattg gttagctatg cataaatggc ttatgcagta
      121 acccaatgtc cagcgccacc agtgttatat agttttactg ctgcggcatc ttgaacactt
      181 tcaggagcat ttgctggtga tacaccttta tatttagcag gtgctactga atcccaagtt
      241 gattgtaaga attgatactt accagctgca cctgatgttg gatttacagc atgaatattg
      301 ccacctgatt cacgttgagc aatttgtttt agatgatcat tcacatttac tgatgaacct
      361 tctgatgctt ttgattcagt tggtgttgca gtaacttgtg aattgtttga tgttgatgct
      421 tgtggttgtt gagtttgagc attttgtggt gcttcaactt cttgtgattg tacttgatta
      481 gattgatcag ctgatggtgc aacattatta gttgcaggtg cttgtgcact catgtctgct
      541 ccattagcac ctgttgcatg gtaattccaa gcaaagtgtg taccatctga ttcaaagtga
      601 taagtaaacc cttcatagtc aaatgtataa ttataagcgc cagcttcaat tggtttttga
      661 tttaatgttt gatcatttga ttgcgccctt tgcgctaaag atgctttatt taagtccgct
      721 tcacttgctt gggcttcgtg acctgcattt cctgctacga ttcctaaacc tactgctaat
      781 gatgatgcga gtaatgtttt cttcataatc ttaaaatcct cctacaagta atttgtttct
      841 ctaaaagttt tacagagact actgtaacat ctgtaaaatc cgtgcaggtt acaacaaaat
      901 aaaaaaatag ccttgttttt tacacagtga ttacaacgga atcctccata atttcagata
      961 tgattctatc gagaattgtc atgacagtaa aaatgttttt aatttgcaat tttttatgta
     1021 atcgttttgc aatctatgtc tattttctat tagaaaaact acacaaaaag acgacctaca
     1081 caacagtact agtcatgtaa gtcgttttta agtgagtata tttcaagtta attagatcta
     1141 taaaaaattt aggatggatt aaatgtatat taaggatgtt taggataatt ggatttaaat
     1201 ttctaataag tcatgattat ctatattttg agagtcaata tctaaaatac tatctgagag
     1261 aatttcatta ttttgggatt taggggacat aaattttatt gtttcaacat ataattcagt
     1321 gacaaagtgt gtttgtccgt ctttatcata ctttcttgat ctcatttgac cagttatacc
     1381 aaccaatgta ccttgattag tgtatctttc tatattagaa gctaacttgc caaatgcttt
     1441 acagaataag taatcacaga cgatttctcc attttcatct ttataatttc ggtgcgttgc
     1501 aacacaaaac gttgcaattt ttctatcctc cttttcaaat atttgtgcgt ctttcgtcag
     1561 tctcccgaca attacgattt tatttagcat gtagtgtgtg attcacctcc tatgcctata
     1621 tcttaattac tagcttaaaa atggtcaaaa tggcaaattt tatataatta atagctatag
     1681 catctcgtta tttgttatat ttaaagaaat tagtcatgaa atgaccttaa atcttctaaa
     1741 taattcaaaa atcataaata ttaaactaat gggatgtggg aatatgaaaa cttttaaagc
     1801 agtaaggttt caaattgtga atgaacacgg cagaattata gaatatgaat tagaagatgg
     1861 cgtaataatt aataaagagg aaagtggcac gggttggctg cttgaaattg tcatttctaa
     1921 tgagcattat gaaacattta aggaatatca agacaatgaa cagttattgg atattcgtgt
     1981 cgtcatcacg cgtccagcca atgatccagc tttatttgaa tctactgtga aaagtattaa
     2041 aaattttaaa acaacaatgt ctatcgtatt tgaatgtcat atttacactt tacgtcaaca
     2101 gtatgctgaa agtttattag aacaacttat tgatgatgga ttatctggtg aagaattaaa
     2161 aaaatcattt aatcgaatga tgcagtctaa accaaagtta aaggatgaaa aattagaaga
     2221 gtaaaataaa aaacattcaa agattatgcc aacacattaa atctttgaat gttttttgtt
     2281 ttattttaca tcttgaattg caaatgtaag ttcacaacta caagcaagtt gaccatcgac
     2341 agtagcttta gcattacctt taccgattgg tcctttaatt ttagtgattt ctacttccaa
     2401 cgttaaagta tcaccaggta ctacttgacg tttaaaacga catttatcaa taccagcaaa
     2461 taaagcgatt ttacctttat tttcttcact atttaaaata gctaccgcac ctgtttgagc
     2521 taacgcttca gttattaata cgcctggcat taccgcatac tcaggaaaat gcccttgaaa
     2581 gaatggttcg tttcctgata cttgtttaat agccacgcaa cgttgacctt cttcatattc
     2641 aacaacttta tcaattaata aaaatggctg tctgtgaggt ataatttgtt taatttggtt
     2701 ataatcaaaa attgtttcca tggttgaaat cctccattat attaatttac tgaattaatc
     2761 gttaatacgt tcaatgtctg cacctaattg cttcaattta ccgtgtaagt caacatagcc
     2821 tctatctagg tgcgttaatt cagtaacgct tgttttacca tcagcaacta atccagctaa
     2881 aattaaggct gctgctgctc ttaaatcagt cgctttaact tgtgcacctt gcaattgact
     2941 tttaccttca agtttagcac tacgaccttc tacattgata ttagcattca tacgtttgaa
     3001 ctctgcaaca tgcataaaac gattttcaaa tacagtttca gttactactt tatgtccgtt
     3061 tgctgttaat aataatgcca tcatttgtga ctgcatatcg gttggaaaac caggatgcgg
     3121 taatgttttg atgtcaacag gctgtaaatc tccttcagca cgtacacgaa taccatcttc
     3181 ttgatattcc aattcaacgc ccatttcttc taatttatac actaaactag ccatatgttc
     3241 tttgattgca ccacgtacaa aaatatcgcc acgagtaatg gcaccagcga tcagtaatgt
     3301 gcctgcttca attctatctg gaatgatagc atgttctaca ccatgtaatg attctacacc
     3361 attgattgta attgtgtctg taccagcacc agtaattcta ccgcccattt cattaatgta
     3421 gtttgctaaa tcaacaattt caggttcttt agctgcattt tcaattaaag tcttaccctt
     3481 agctaatgat gctgccataa taatattttg tgttgctcct acacttggaa aatctaaatg
     3541 aattgatgta ccttttaatc catctttagc attagcataa atattaccat tttcaagatg
     3601 aatttctgcg cctaaagctt caaaaccttt aatgtgttgc tcaatcggtc tacttccaat
     3661 tgcacaacca ccaggcaatg caacaatagc atgtcctagt cttgctaaaa gaggtcccat
     3721 aactaaaata cttgcacgca ttttactaac atattcatat ggtgcctctt catttagagt
     3781 ctttgttgca tcaacgacaa cagcattttc gtcctttttg tatgtaacgt cagcatttaa
     3841 ggttgttaat acattattta ttgtttctac atcacttaaa gctggaacat taactaattt
     3901 gctcggttta tcagaagcta ataaagatgc tgttaatata ggtaatactg catttttagc
     3961 accttctact ttaacttcac ccgttaattt atttccacct ttgattacta ttttatccat
     4021 cgtaaatcct ccacttagaa ctcctattac tttacatttc cctattttaa aattatttta
     4081 agtcattatt taattatatc tagttttgat tacaaatcat agagttagac ttattttatt
     4141 aaatatttta cctgcgtcga gtattgtaac aaatctacaa taaaattgct tactgcagtg
     4201 cctaataaaa tagcaacaaa tatcatacac acttgtaatt gagtggcata tcctttttta
     4261 aaaaattgat ctaatctaat tgattgtaaa gcccaatagg caatacaaat acatacaaca
     4321 tgtaacacta aatggataac tgcatattgt ccgatataat ccattttatc acctcataag
     4381 attactgtat tacattgtac atgaaaaatt taaataatga tacaaagttt aatacccgtt
     4441 tttagtaaag tttaagctaa cttactatat aattcttttt acataaatat cataactata
     4501 aagtatcttg gcatggaaaa atgtagctac tttcaaaaaa agaaacgtcg actgtagata
     4561 gtggtgaaag gaaattttga ttttagcaat ctattaatga ttgtaaaaag taaaactatt
     4621 cttgggagtc gaacataaat cgatgtctag actctatagt gttatatttg gcagtagttg
     4681 acggggcccc aacataaaga aattggattc ccaatttcta cagacaatgc aagttggggg
     4741 cggggcccca acacaaaagc tggcggaaag tcagcttaca ttaatgtgca agttggggtg
     4801 ggccccaaca taaagaaata ctttttcttt agaaattagt atttctta
//
LOCUS       DAMZPP010000170         4801 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMZPP010000170 DAMZPP010000000
VERSION     DAMZPP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.171"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..519
                     /locus_tag="POH73_002524"
     CDS             <1..519
                     /locus_tag="POH73_002524"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDI6888434.1"
                     /translation="PLAGPVVACATILNSNHNYLGLDDSKKVPVTKRLELNEALKNEV
                     TAFAYGIATAEEIDEFNIYKATQIAMQRAIDGLSVQPTHLLIDAMTLDNALPQVSLIK
                     GDARSVSIAAASIMAKVFRDDYMTQLSKDYPEYGFEKNAGYGTKQHLLAIDDIGIMKE
                     HRKSFEPIKSLL"
     gene            628..1794
                     /gene="sucC"
                     /locus_tag="POH73_002525"
     CDS             628..1794
                     /gene="sucC"
                     /locus_tag="POH73_002525"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDI6888435.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            1816..2724
                     /gene="sucD"
                     /locus_tag="POH73_002526"
     CDS             1816..2724
                     /gene="sucD"
                     /locus_tag="POH73_002526"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDI6888436.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYELLLTVNK"
     gene            2951..4069
                     /locus_tag="POH73_002527"
     CDS             2951..4069
                     /locus_tag="POH73_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDI6888437.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEAVVKTSDTSLKLDNKSAISNGNEINQD
                     MKISNTPKNSNQGNNLVVNNNEPTKEIKIANLEAQNSNQKKVNKGTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVPSNNKSNSNNSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
     gene            4097..>4801
                     /gene="fmhC"
                     /locus_tag="POH73_002528"
     CDS             4097..>4801
                     /gene="fmhC"
                     /locus_tag="POH73_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HDI6888438.1"
                     /translation="MKFSTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINHEAHIVGV
                     KNDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDAKLVCYFFKELSKFIYKNRGV
                     FILVDPYLIENLRDANGRIIKNYNNSVIVKMLGKIGYLHQGYTTGYSNKSQIRWISVL
                     DLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEETNRFYKLFQMAEEKHGFHF
                     MNEDYFKRMQEIYKDKA"
BASE COUNT         1818 a          629 c          942 g         1412 t
ORIGIN      
        1 cctttagcag gtccagtcgt tgcatgcgca acaattttaa attcaaatca caattatttg
       61 ggccttgatg attcgaaaaa agtacctgtt acgaaacgtc tagaattaaa tgaagcacta
      121 aaaaatgaag ttactgcttt tgcatatggt atcgcgacag ctgaagaaat agatgaattt
      181 aatatttata aagctactca aatcgccatg cagcgagcta ttgatggatt atcagtacaa
      241 ccaacgcatt tattgataga cgcgatgacg cttgataatg cactgcctca agtatcttta
      301 atcaagggtg atgcaagaag tgtgtctatt gcagctgcaa gcatcatggc aaaggttttt
      361 cgtgatgatt atatgacaca gttatctaaa gattatcctg aatatggttt tgaaaaaaat
      421 gcgggttacg gtaccaaaca acatttacta gcaatcgatg atattggcat tatgaaagag
      481 catagaaaaa gctttgaacc tataaaatcg ttactgtaaa tcgtaaaatt caattaaatg
      541 ataaaaaagt agcatttaag tttacaatag cgcttacatt ttttataatt gtaagggaac
      601 ttaacctaag taacaggagg atggaagatg aatatccacg agtatcaagg taaagaaata
      661 tttcgttcaa tgggcgttgc agttccagaa ggacgagtag catttactgc tgaagaagcg
      721 gtggagaaag caaaagaatt aaattctgat gtatatgttg tgaaagcaca aattcatgct
      781 ggaggtagag gtaaagcagg cggagtaaaa attgctaaat ctttatctga ggtagaaaca
      841 tatgcaaaag aattattagg gaaaactttg gtgacacatc aaactggtcc agaaggtaaa
      901 gaaattaagc gtttatatat cgaagaaggt tgtgctattc aaaaagaata ttacgttgga
      961 ttcgttattg atcgtgcgac tgaccaagta acattgatgg cgtctgaaga agggggcact
     1021 gagattgaag aagttgctgc gaagactcct gaaaagatct tcaaagaaac tatcgatcca
     1081 gtaatcggac tttcaccatt ccaagcaaga cgaattgcgt ttaatattaa tattcctaaa
     1141 gaatctgtta acaaagctgc taaattctta ttagcacttt ataatgtatt cattgaaaaa
     1201 gattgttcaa tcgtagaaat caacccatta gttacaacag ctgatggtga tgtattggca
     1261 ttagatgcta aaattaattt tgatgataat gcattattca gacataaaga tgttgtagaa
     1321 ttacgtgatt tagaagaaga agatccgaaa gagattgaag cgtctaaaca tgatttatca
     1381 tacattgcat tagatggtga catcgggtgt atggttaatg gtgcaggttt agccatggca
     1441 acaatggata cgattaatca tttcggtgga aacccagcca atttcttaga tgcaggcgga
     1501 agcgctacta gagaaaaagt aactgaagca tttaaaatca ttttaggtga tgaaaatgtt
     1561 aaaggtattt ttgtaaacat tttcggtggc attatgaaat gtgatgttat cgcagaaggt
     1621 atcgttgaag ctgtaaaaga agtagattta actttaccac tagttgtacg cttagaaggt
     1681 acaaatgttg agttaggtaa aaaaatctta aaagactcag gattagcaat tgaaccagca
     1741 gcaacaatgg ctgaaggtgc acaaaaaatt gttaaactag tcaaagaagc ataaggaaag
     1801 gatgggagca ctaagatgag tgtatttata gataagaata ctaaagtaat ggtacaaggt
     1861 attacagggt ctactgccct tttccataca aaacaaatgc ttgattatgg tacgaaaata
     1921 gtagcaggtg tgacgcctgg taaaggtggt caagttgttg aaggcgttcc tgttttcaac
     1981 actgttgaag aagctaaaaa tgaaactggg gcaacggttt cagtcattta cgttccagca
     2041 ccatttgctg cagactcaat tttagaagca gctgatgcag acttagatat ggttatttgt
     2101 atcactgaac atattcctgt attagacatg gttaaagtta aacgctattt acaaggtaga
     2161 aaaacacgtt tagttggtcc aaactgtcca ggtgtgatta cagcagatga atgtaaaatt
     2221 ggtattatgc ctggctatat tcacaaaaaa ggtcatgttg gtgtagtatc acgttcaggt
     2281 acactaacat atgaagcagt gcaccaattg actgaagaag gtattggtca aactacagct
     2341 gttggtattg gtggagaccc agtaaatggc acaaacttta ttgatgtttt aaaagcattc
     2401 aatgaagatg acgaaacgaa agcagttgtt atgattggtg aaatcggtgg tacagctgaa
     2461 gaagaagcag ctgaatggat taaagcgaat atgacaaaac cagttgtagg ctttatcggt
     2521 ggacaaacag cacctcctgg aaaacgtatg ggacatgctg gtgcgatcat ttcaggtggt
     2581 aaaggtactg ctgaagagaa aattaaaaca ttaaatagtt gtggtgtgaa aacagcggca
     2641 acaccttcag aaattggttc aacattaatt gaagctgcta aagaagcagg tatttatgaa
     2701 ttattattaa cagttaataa ataaagttaa aagatgatat aaatggctat agcccattcc
     2761 atactttata aaagtatcgg aaatgggcta tttgtcgtgc gatgaattta agtacacttt
     2821 tcatattttg aaaaggcaag ttaattattt tacctccttc aatagctttc taaaacaaag
     2881 caaattttta ttaaaatata tagtgcacta tgttataatg tttatatatt attgtaagga
     2941 gtgtttcgtc atgaataaac aacaaagtaa agtacgctat tcaattagaa aagttagtat
     3001 tggaattttg tcaatttcaa taggtatgtt tttggcattg ggtatgtcga acaaagcata
     3061 tgcagatgaa attgataaat ctaaagattt tacaagaggg tatgagcaaa atgtattcgc
     3121 gaaatcagag ttaaatgcta ataaaaatac gacaaaagac aaaataaaaa atgaagctgt
     3181 tgttaaaaca tcggacacaa gtttaaagtt agacaacaaa tcagcaattt caaatggaaa
     3241 tgaaattaat caagatatga agatttcaaa tactccgaaa aactcaaacc aaggtaacaa
     3301 tctagttgtt aataacaatg aacctactaa agaaattaaa attgcaaact tggaagctca
     3361 aaattctaat cagaagaaag tgaataaagg tactaataat tactttggtt actacagttt
     3421 tagagaagct ccaaaaacac aaatctatac tgtaaaaaaa ggtgacacac ttagtgctat
     3481 agcattaaaa tacaaaacta cagtttcaaa tattcaaaat acaaataata tagcaaatcc
     3541 taatttaata tttattggtc aaaaattaaa agtgccaatg acaccattag tagaaccaaa
     3601 accaaaaaca gtgccttcaa ataataaaag taatagtaat aacagtacat taaattattt
     3661 gaaaacatta gagaatagag gatgggattt cgacggtagt tatggatggc aatgtttcga
     3721 tttagtcaat gtgtattgga atcatcttta tggtcatgga ttaaaaggat atggagctaa
     3781 agatatacca tatgcaaata attttaatag tgaagctaaa atttatcaca acacaccaac
     3841 tttcaaagct gaacctgggg acttagtggt ttttagtgga agatttggtg gaggatatgg
     3901 tcatacagct attgtcttaa atggtgatta tgatggaaaa ttaatgaagt tccaaagttt
     3961 agatcaaaac tggaataatg gtggatggcg taaagcagag gttgcacata aagttgttca
     4021 taattatgaa aatgatatga tttttattag accatttaaa aaagcataat ttaaatcgaa
     4081 ggcaggacat tgaaatatga aattttcaac tttaagtgaa gaagaattta ccaactacac
     4141 caaaaagcac ttcaaacatt atacacagtc tatagaatta tataattata gaaataaaat
     4201 aaatcatgaa gcacatattg tgggagtgaa gaatgataaa aatgaagtta tagctgcatg
     4261 tttattaaca gaggcacgta tttttaaatt ctacaaatat ttctactctc atagaggtcc
     4321 tttacttgat tatttcgatg ctaaattagt ttgttacttt tttaaagaat tatctaaatt
     4381 catttataaa aatagaggtg tatttattct tgttgatcca tatttaatag agaatttaag
     4441 agatgcgaat ggtaggataa taaagaatta taataattca gtgatagtaa agatgctagg
     4501 aaaaattggg tatctccatc aaggttatac aacaggatat tcaaataaaa gccaaattag
     4561 gtggatttct gtattggatt taaaagataa agatgagaat caacttttaa aagaaatgga
     4621 ataccaaact agaagaaata taaaaaagac tattgagatt ggtgttaagg ttgaagattt
     4681 atctattgaa gaaacaaatc gattttataa attgtttcaa atggctgaag aaaaacatgg
     4741 ttttcatttc atgaatgaag attattttaa acgaatgcaa gaaatatata aagataaggc
     4801 a
//
LOCUS       DAMZPP010000171         4783 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMZPP010000171 DAMZPP010000000
VERSION     DAMZPP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.172"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            260..3214
                     /gene="fdhF"
                     /locus_tag="POH73_002529"
     CDS             260..3214
                     /gene="fdhF"
                     /locus_tag="POH73_002529"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDI6888439.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTSIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIEEWVEDFDEYYK
                     SLETFTMAFAEEATGIPEAELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGQIHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALKPLGDSKPDWKIFQAIANKLS
                     FDWNYKHPSEIMDEIARLTPLYAGVSYERLEGFNSLQWPVHPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEVYDIHVNNGRLLEHFHEGNMTYQTPMLKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRMEVITKRGKSPLNPNNFRVNKKRHPQYSVQVQKKWER
                     PDYVFPGNQVDK"
     gene            3214..3687
                     /locus_tag="POH73_002530"
     CDS             3214..3687
                     /locus_tag="POH73_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HDI6888440.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAVRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTSVRSLLGVLKDDDMNRSLTYMLNMLKGMSRED"
     gene            3981..4214
                     /locus_tag="POH73_002531"
     CDS             3981..4214
                     /locus_tag="POH73_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888441.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            4236..>4783
                     /locus_tag="POH73_002532"
     CDS             4236..>4783
                     /locus_tag="POH73_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDI6888442.1"
                     /translation="MKKNFKLRISTLLLIVILVVFSVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQ"
BASE COUNT         1705 a          646 c          960 g         1472 t
ORIGIN      
        1 tgaaacagat aattggaaaa ctaataatga taattatttt caattaaaca aaaagatgat
       61 ttaatttcgc gcaaaatatt tcaaataaaa atgattgcaa aaataaaaaa tacaaaaata
      121 cgcttaacat tatacactta aagtaataca attatattga ttctatctga aagatgtgtg
      181 gggcatcttt attttaggtg gaaattaggc aatttattaa aagtcattta cggaaaaata
      241 tatagatggg gtgaatagta tgcaagaaca tttggtggtc acgcttgatg gtaaagatta
      301 tcttgtagaa ccaggtacaa atttacttga attcattaaa tcgcaagata cttttgtgcc
      361 ttcaatttgt tataacgaat caatgggacc gatacaaacg tgtgatacat gtacagttga
      421 gattgatggt aaaattgaac gttcatgtag tacagtgatt gatcgtccaa tgacagtaaa
      481 tacagtgaat aatgacgtga aagatgctca aaaagaagcg cttgatcgaa ttttagaaaa
      541 gcatatgctg tattgtacag tatgtgatta taataatggt gattgtgaaa ttcataatac
      601 gatggatgca tgggggcttc agcatcaaac gtatgaatat aaagagaagc catatgaaaa
      661 agattatggc ccattttatc gctatgatcc aaatcaatgt atcttatgtg gccgttgtgt
      721 agaagcgtgt caagatattg aagtaaatga aacaattaga attgattggg atcgtgagca
      781 tccacgtgtc atttgggata atgatgtacc gattaatgaa tcgtcatgtg tatcttgtgg
      841 tcaatgcgca actgtatgcc catgtaatgc gatgatggaa gttaacatgg aaggtaatgc
      901 aggttatatg actgatacgg aacctggttc attagcggca atgattgatt taactaaaaa
      961 agcagaacct ggttatggtc cactatttgc aatctcagat tcagaagcag aaatgcgtaa
     1021 agagcgtatt aagaaaacta aaacagtgtg tacatattgt ggtgtgggct gttcatttga
     1081 agtttggact aaagatagag aaattttaaa agtacaacca tcacatgatt ctccagcaaa
     1141 taaaattgca acttgtgtta aaggtaagtt ttcatgggga catattaatt cagatcaacg
     1201 cttaacgaaa ccattagtaa ggaaaaatgg tgagttccat gaagtagaat gggatgaagc
     1261 attaaatgtc attgcagata attttacatc tattaaagaa aagcatggtc cagatgcatt
     1321 atcattcatt tcttcttcta aagcgacgaa tgaagaatcg tatttaatgc aaaaattagc
     1381 aagacaagtt attggcacaa ataacgttga taactgttca agatattgcc aagcacctgc
     1441 aacaaaaggc ttatttagaa cggttggaca cggcggtgac tcaggtagta ttgaagattt
     1501 agaaaaagca gcaatgtctg tattgattgg tacgaataca gctgaagcac atccggttat
     1561 tgcatcacgc atgaagcgtg ctcaaaaatt atttggacaa aaaatacatg tgtttgatat
     1621 tagaaaacat gaaatggctg aacgcgcaga tcgtttttat caaccgaaac caggtacaga
     1681 tttggcttgg ttaagcgcag tgacgaaata tatcattgat catgacttac acgataaagc
     1741 ctttatcgaa gagtgggttg aagattttga tgaatattac aagtcattag aaacatttac
     1801 aatggcattc gctgaagagg ctactggtat tccagaagca gagttgatta agttcgctga
     1861 agaatgtgcc aaagctgagt ccgttgtcat ttgttgggca atgggtatca cacaacaaga
     1921 cattggaagt gattcaagta cagcaatttc aaacttatta ttagttactg gaaattatcg
     1981 tcgacctggt acaggcgcgt atccattacg tggacataat aatgttcaag gttgtagtga
     2041 tatgggtagt atgcctgata aaattactgg ctatcaaagt attgaagcgg acgatattcg
     2101 cgctaaattc gaaaaagaat atggcgttaa attgaatccg aaagctggta aagataatca
     2161 tgaaatggtt gaaggtatcc atgatgggca aattcactca ttatatctat atggagaaga
     2221 tactggtatt gtagattcaa atattaattt tgtacaagct gcttttgaaa aattagattt
     2281 catggtagtc caagatgaat ttttaacatt cacagcaaca tatgcagatg ttgtcttgcc
     2341 agcaagtcct tcattagaaa aagatggtac ttttacaaat acagagcgac gtattcaacg
     2401 tttataccaa gcattgaaac cattaggtga ttctaaacct gactggaaaa tattccaagc
     2461 aattgctaat aaattaagtt tcgattggaa ttacaaacat ccaagcgaaa ttatggatga
     2521 aattgctaga ctaacaccat tatatgctgg cgtaagctac gaacgtttag aaggatttaa
     2581 tagtttacaa tggcctgtgc atccagatgg tacagatgaa ccaatacttt atttagaagg
     2641 atttaatttc gataatggta aagcaaaatt attcccatta tcatttgata attactttaa
     2701 gcaagatgaa gtttatgata ttcatgtaaa caatggtcga ttgttagagc acttccacga
     2761 aggtaacatg acatatcaaa caccaatgct taaatacaaa gtgccacgtg catttgttga
     2821 aatttctcca gaacttgctg aagatagagg cattcatgaa ggtgcagaag ttaagttgat
     2881 atctgaaaca ggagaagcgg tattacaagt tcacgtgaca gatcgtgtta aaggtaaaga
     2941 aatctatatt ccattaaata atgatgcgat ggaaaatgga gatctaggcg caattaactt
     3001 attaactaat agtgatgttg atcaatacac ggatacacca tcttataaac gtacaagctg
     3061 tcgtatggag gtaattacga aacgtggtaa gtcaccgttg aaccctaata atttccgtgt
     3121 caataaaaaa cgtcatccgc agtacagtgt tcaagtacag aaaaaatggg aacgtccaga
     3181 ttatgttttc ccgggaaatc aggtggataa ataatggctg agagaatatc ttctaaaatt
     3241 agacgcttag aaaaatcaga agaacaaatt aagttagaaa gtttaaatga agttacagag
     3301 gcaattgctg caaacaaaga tagcatttta aaagcaatca agctcattaa aacattagat
     3361 gatgcaaaac tattagatgc tttaaatggc gcagttagag gacgtcaagt gattattaat
     3421 aaatttgctg ttgagctcaa caaagatatt tatacagggc tattatctaa tatggcttca
     3481 atggtatttt tattaggcga attgaatgta tcagatttaa gtgacttcct taataaagtt
     3541 aacaaaggtt tacacgttgc caatcaagcg agtcctaatg cgaagacatc tgtaagaagt
     3601 ctattaggcg ttttaaaaga tgacgacatg aaccgtagtt taacatatat gcttaatatg
     3661 ttaaaaggta tgtctcgaga agattaatat gagtctataa taaatcggat tgatgtaaaa
     3721 gttttttact tttcatcttt ccgatttttt gtttttgaat ataacagtgt ttctgcatgt
     3781 cggtgtaggg gcactgtttt aagttatatt gaaatattat ttgtatccga gtcttcatca
     3841 aacttcaaac aatatttttg agaatcgaga aaatcatgtg ctattgataa tcaaccatcg
     3901 acagggctat gaaacatcat gtattattat gattatgtaa gttatttaaa taaaaagggt
     3961 gagtgcatat taattcgaga atgttaaaaa cgtcagctgt aatttctaat acattattag
     4021 tcataggttt agtctgtcta tttatgatga aattagtgtt agctattact ttttttgctg
     4081 tttcattatc aattagtcta gttgtcttta atatgatgtt cagagataga acaacgatga
     4141 aagtaatcgt taacgcttct tttttaatcg taatattagc gattgtagtg gcatattttt
     4201 tattgtctaa gtaagaaaaa tgaggttttg tctatatgaa gaagaatttc aagttacgta
     4261 tttcaacgct actattgata gttattttag ttgttttttc tgtattgctc atcgtgaatg
     4321 aaactaaatt gtttaaaaat gatgtgaatt actcttttga tgaggctgtt tcaatgcaac
     4381 aagggaaagg tattgtacag acaaaagaag aggatggtaa atttgttgaa gcaaataata
     4441 atgaaattgc taaagcaatg actatttcac ataaagacaa tgatatgaag tatatggata
     4501 tcacagaaaa agtgccaatg tcggaatctg aagttaatca attgctaaaa ggtaagggga
     4561 ttttagaaaa tcgagggaaa gtttttctag aagctcaaga aaaatatgag gttaatgtca
     4621 tttatcttgt tagccatgca ttagtagaaa caggtaacgg caaatcagaa ttagcaaaag
     4681 gcattaaaga tggaaaaaaa cgctattaca acttttttgg tataggagca ttcgatagta
     4741 gtgctgttcg tagtgggaaa agttatgctg aaaaggaaca atg
//
LOCUS       DAMZPP010000172         4727 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMZPP010000172 DAMZPP010000000
VERSION     DAMZPP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.173"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            118..1287
                     /locus_tag="POH73_002533"
     CDS             118..1287
                     /locus_tag="POH73_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HDI6888443.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLGQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VIMIIGAVILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFSNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKHS"
     gene            complement(1395..1823)
                     /locus_tag="POH73_002534"
     CDS             complement(1395..1823)
                     /locus_tag="POH73_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HDI6888444.1"
                     /translation="MILNFNQFENQNFFNGNPSDTFKDLGKQVFNYFTTPSFVTNIYE
                     TDELYYLEAELAGANKEDISIDFNNNVLTIQATRSAKYKSGKLILDERNFESLIRQFD
                     FEAVDKQNITASFENGLLTITLPKIKPSNDTTSSTSIPIS"
     gene            complement(2040..2486)
                     /locus_tag="POH73_002535"
     CDS             complement(2040..2486)
                     /locus_tag="POH73_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6888445.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKDYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIVNNLRNFVSKNFDNSDKK"
     gene            2883..3248
                     /locus_tag="POH73_002536"
     CDS             2883..3248
                     /locus_tag="POH73_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDI6888446.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            3430..4707
                     /locus_tag="POH73_002537"
     CDS             3430..4707
                     /locus_tag="POH73_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDI6888447.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKDITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
BASE COUNT         1577 a          637 c          816 g         1697 t
ORIGIN      
        1 gtagagcatg gaacattgat ttatttccca ggctctttaa ataacattga cctctaacat
       61 ataggcagag tattgatatc tattaaaaaa taaatgattt tggtgaaggt gaaacgtatg
      121 tacaaaacaa aaggtggctt tcaacttaca ttacaaacat taagtttagt ggttggattt
      181 atggcttgga gtattattgc tcctttaatg ccttttatta aacaggatgt caatgtaact
      241 gaaggtcaaa tatcaatcat tttagcgata ccagttattt tgggatcagt gctccgtgtg
      301 ccatttggtt atttaacaaa tattgttggt gctaaatggg tattctttac aagttttatc
      361 gtattgttat tcccgatatt tttcttgggc caagcacaaa cgccggggat gttaatggca
      421 tcaggatttt tccttggcgt aggtggcgca attttctctg ttggtgttac atcagttcct
      481 aaatatttcc ctaaagaaaa agtaggtcta gcaaatggta tttatggtat ggggaatatc
      541 ggtacagcag tttcttcatt tttagcacca ccgatagcgg gtattattgg ttggcaaaca
      601 acagttagaa gttacttaat tattatcgct ttatttgcat taattatgtt catttttggt
      661 gacacacaag aacgtaaaat taaagtgcca ttaatggccc aaatgaaaac attatctaaa
      721 aactacaaat tatattactt aagttattgg tatttcatta cttttggtgc ttttgttgca
      781 tttggtattt tcttacctaa ctacttagta aatcattttg gaattgacaa agtagatgct
      841 ggtattcgtt caggtgtatt cattgcgctg gcaacattct tgagaccaat aggtggtata
      901 ctaggtgata aatttaatgc agttaaagta ctgatgattg attttgttat tatgattatc
      961 ggtgccgtta ttttaggtat ttcagaccat atcgcattat ttactgtagg ttgtttaaca
     1021 ataagtattt gtgcaggtat tggtaacggc ttaattttta aattagtacc atcatacttc
     1081 tcaaatgaag caggatcagc aaatggtatc gtatcaatga tgggtggttt aggaggattc
     1141 ttcccaccgt tggtcatcac atacgtagct aatttaacag gttcaagcca tttagcattt
     1201 attttcttag cggtattcgg atgcattgca ttatttacaa tgcgccattt atatcaaaaa
     1261 gaatatggtt cattgaaaca cagttgatat gtaatacgaa gcatttttaa atttaaatga
     1321 taaagcctta ataccatgtg taacgttagt agcatgacat taaggcttta gtaatgtatt
     1381 cattttaaat ttaactaaga aattggaata gatgttgatg aagtagtgtc attgcttggt
     1441 ttgattttag gcagggtaat ggttaatagc ccattttcaa aactagcagt aatattttgc
     1501 ttatcaacag cttcaaaatc aaattggcgt attaatgatt cgaagtttcg ctcatctaaa
     1561 atgagttttc cggatttata tttagcactt ctagtagctt gaatagtaag cacattatta
     1621 ttgaaatcga tactaatgtc ttctttattt gcacctgcta gctcagcttc taagtaatat
     1681 aattcgtctg tttcatatat attcgttaca aatgaaggtg ttgtaaagta attaaatact
     1741 tgtttaccta aatctttaaa tgtatcactt ggattaccgt taaaaaagtt ttgattctcg
     1801 aattgattga agttcaatat catcacttcc attatataaa tttgtattac aaagtcatta
     1861 tatgagtaga ataaaaaaag ataaagtaaa agggggggat aaaaaagtta taaatggata
     1921 gatttgtgcc tgataaggaa tcagggcaaa agaactgagc atcgagattt atacaagcaa
     1981 taaagataat gaataaacat agcgatgcat acttctgccc atcatcttat gatagggagt
     2041 tactttttgt cggaattgtc gaaatttttc gaaacaaaat tccgcaaatt attaacaatg
     2101 tctgatgctt ctcgttctga aatgttgaat tcattaaata ctttaacaga aatttcagca
     2161 agaggacttt ttattgcttt accttgttct gttagagaaa tttgtaggtt tctttcatct
     2221 ttctcttcac gtgttcgaac aacgtaatct ttcttttcta atttctttag taatggtgtc
     2281 agtgttccag aatctaagaa tacacgttca cctaattttt tgatgttaag tttttcatca
     2341 ttttcaatcg ccattaaaac aatgtaacca gtataggtta aatcataatc cttaagatag
     2401 tttgtgtatt ttttaattat ttcttttgaa gaaacataaa ataaaaaaca caactgtttg
     2461 ctaagatagc tgttttctac atacatccaa tcactccttg ttaaaataaa caatattatg
     2521 aagtgtaata gttgtcaagt actgatttca tcgcaattat cgaattgtct ataattgaat
     2581 ttaaatgtta aataatgcaa aacatatcaa tatcttagta ttttcatcgg taatgactga
     2641 tatgactgat tttttgaatt gaaaaaaatg taattgtatc attcttaatt gttaaaagta
     2701 gtcaattatt ttgatagata gtaaaatgaa atttgttatt aaatttttta aaagcatcgt
     2761 gtatgtaatt cgatttctat aataatattg agaagaaaaa aattgttaca atctggtaaa
     2821 aactctttat taaataaatt gattcgttca tactgaatag ataagcaaaa aggagttaca
     2881 taatgaagaa gaaaaaaaac gcgattaaag ttatcattat attatttatt tcattaattg
     2941 ttgctgtagc atttttcttt ggtctgaaaa catatcaagg ccataagaat attcaactta
     3001 ttgacagtta cttggaagag aaaaatctaa aagataaaat taaatcagaa aaaacagagt
     3061 atagtgctaa aaaaggtctc ttctataagg aagttacgtt taaagatgaa cctggtgtaa
     3121 cttatgttgt gcaaccaatc agtactaata aaggattatt tgttgaaggc tttgatacag
     3181 agactaagaa aagtttgaaa actgcaaagc ataaatattt caatcagaat tataaaccat
     3241 caaaatagtg aaacgtataa ctggtgaaac agtttagtgc ctgcaatttt aattatttaa
     3301 ggtaaacagt ttttgtatgg aaattagtga tttttgacat ctataaaaga taaatatttt
     3361 aattttttat agataattat caattttctg atataattga gtgatgtcag atgaagggga
     3421 gtttgtacta tggctctatt caagagaaaa attagcctac ctatgcaagt agttattgca
     3481 ttagtgttag gtgttgtcgt aggactttta ttatatggac aagaaaacgt agcaaattac
     3541 attaaaccat ttggtgacgt atttttaaat ttaattaaaa tgatcgttat accagttgta
     3601 ttttgctcac tagcgctttc tatttcgaac gttggggaat cgaaaactgt agggcgttat
     3661 ggctggaaaa caattttata ctttgaaatt attacaacaa tcgcaatagg tttagggatt
     3721 atcttcggta acctatttaa accaggtgct ggattagacc caacaaaatt acctaaaggt
     3781 gatatttcta aatatcaatc aactgcacat gcagcagaac aatctacata tggaaatcat
     3841 tttattgata ccattgtaca tattattccg acaaactttt ttgaagcttt aaataagggt
     3901 gaattattac ctattatctt tttcgcagta ttctttggat taggattagc tgcagtaggt
     3961 aaaaaagcag aaccagttaa agaattttta agcggatcgc ttgaagctgt gttctggatg
     4021 attaataaaa ttttaaaatt agcaccactt ggagtgtttg cattcatttg tactacaatt
     4081 attacatttg gtgcatcagc attattacca ctattaaaat tagtattagt tgttgtcttt
     4141 gcaatggtgt tctttgtatt cgctatacta ggactagttg catggatgtg tggtattaat
     4201 atcatgaata ttattagaat cttgaaaagt gaattgcttt tagcattttc tacatcaagt
     4261 tcggaagctg ttcttcctgt aatgatgaag aaaatggaaa actttggttc accaaaagat
     4321 attacatcct ttgttatacc gattggctat acgtttaact tagacggatc agcactttat
     4381 caatctattg cagcattatt cgttgcacag atgtatggaa tgcacttaac attatcagag
     4441 caaattgtgt tgatgttaac attaatgatt acatctaaag gtatggcggc agtaccagga
     4501 acttcaatcg ttgttttatt aacaacatta ggcgccatgg gcttaccggc acaaggttta
     4561 gcattaatta ttggtgttga ccgtatctta gatatggtac gtacatgtgt aaacgttatt
     4621 ggtaacgcat tatcaacaat tgttattgct aaatgggaaa acgtatatga taaagcaaaa
     4681 ggtcaagaat atttaaaatc aatttaaaaa atactatctg acattta
//
LOCUS       DAMZPP010000173         4576 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMZPP010000173 DAMZPP010000000
VERSION     DAMZPP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.174"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(96..485)
                     /locus_tag="POH73_002538"
     CDS             complement(96..485)
                     /locus_tag="POH73_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HDI6888448.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGGTPYFKPLHMVLRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWI
                     TIALIIITMVLGVILPLGPISKLFGIG"
     gene            complement(811..1713)
                     /locus_tag="POH73_002539"
     CDS             complement(811..1713)
                     /locus_tag="POH73_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDI6888449.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVERLYVFTKAASSLTGDNAKIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            complement(1879..>4576)
                     /gene="addA"
                     /locus_tag="POH73_002540"
     CDS             complement(1879..>4576)
                     /gene="addA"
                     /locus_tag="POH73_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDI6888450.1"
                     /translation="DYFSREADDLKADMQQLAPRVKYLARIVKDVMSEFNRKKRSKNI
                     LDFSDYEHFALQILTNEDGSPSEIAESYRQHFHEILVDEYQDTNRVQEKILSCIKTGD
                     EHNGNLFMVGDVKQSIYKFRQADPSLFIEKYQRFTLDGDGTGRRIDLSQNFRSRKEVL
                     STTNYIFKHMMDEQVGEVRYDEAAQLYYGAPYDESDHPVNLKVLVEADQEHSDLTGSE
                     QEAHFIVEQVKDILEHQKVFDMKTGSYRSATYKDIVILERSFGQARNLQQAFKNEDIP
                     FHVNSREGYFEQTEVRLVLSFLRAIDNPLQDIYLVGLMRSVIYQFKEDELAQIRILSP
                     NDDYFYQSIVNYINDEAADAILVDKLKMFLSDIQSYQQYSKDHPVYQLIDKFYNDHYV
                     IQYFSGLIGGRGRRANLYGLFNKAIEFENSSFRGLYQFIRFIDELIERGKDFGEENVV
                     GPNDNVVRMMTIHSSKGLEFPFVIYSGLSKDFNKRDLKQPVILNQQFGLGMDYFDVDK
                     EMAFPSLASVAYKAVAEKELVSEEMRLVYVALTRAKEQLYLIGRVKNDKSLLELEQLS
                     ISGEHIAVNERLTSPNPFHLIYSILSKHQSASIPDDLKFEKDIAQIEDSSRPNVNISI
                     IYFEDVSTETILDNDEYRSVNQLETMQNGNEDVKAQIKHQLDYQYPYVNDTKKPSKQS
                     VSELKRQYETEESGTSYERVRQYRIGFSTYERPKFLSEQGKRKANEIGTLMHTVMQHL
                     PFKKERISEVELHQYIDGLIDKHIIEADTKKDIRMDEIMTFINSELYSIIAEAEQVYR
                     ELPFVVNQALVDQLPQGDEDVSIIQGMIDLIFVKDGVHYFVDYKTDAFNRRRGMTDEE
                     IGTQLKNKYKIQMKYYQNTLQTILNKEVKGYLYFFKFGTLQL"
BASE COUNT         1460 a          831 c          610 g         1675 t
ORIGIN      
        1 tttttttgtt taaacatttt tagaatcaaa aaagacataa ggtcttggac taataattgt
       61 ccatgcctta tgtcatatac tatatgtctt atcaattagc caataccgaa taattttgat
      121 ataggcccta acggtagaat gacacctaat accattgtga tgataattaa tgcaattgtt
      181 atccaaaaca ttgtgtgact ttgttcatgt ctctttcttt tagcaatcga cacttccatc
      241 aatccaacta ctgctacacc acacagcatt ttcaatgtaa gcaacatatg atttgccccg
      301 ccattcataa atgactgaat taatatccaa aatcctgaaa ttaacgtcaa cagcataaat
      361 aatcgtagca ccatatgtaa tggtttgaaa tatggcgtac caccttgatt ttttgaaata
      421 tttaagtatg tagcgataaa taaaatgatt gctaataccc aacttaaaat atgtaaatgt
      481 aacatattaa ttcccccact ttaataattt tagttattaa attaaaactt cgtgggataa
      541 atacccactt tcttataatt taatcattat ttgttcatac acaaatatat attttaccac
      601 acttctatat tttagaggaa gaacatgaca ttttattact tattagaata ctgtgcatct
      661 actgtaaaat aatcaactac ttcttaatat caatatttca ctttcgtctc aaaatggtaa
      721 cattataaac aatgatcttt aacacttttt agaaaagcaa gaaaaaacta accaatctat
      781 ataaagactg gttagctttt taaatgataa ttatttagcg atataagttg tcagcgttcc
      841 aatattatca atagtcactt taacttcatc acctggttgt aagaattttg gtggttgcat
      901 acctgcacca acgcctgctg gtgtaccagt tgcaataata tctcccggat gtagtgcaac
      961 atattttgaa atttcttcta ttaattcatc aattttaaga atcatttcgc cagtgttgcc
     1021 atcttgtcta atttcgttgt taacttttgt aacaatattt acattttcag gtaatggtag
     1081 ttcgtcttta gtaacgatat aaggacccat tgggcaaccg ccagttaaac tttttgataa
     1141 aaatgcttga tcttgttcac tttgtgcttt gcgatcagtg atatcgttaa taattgtata
     1201 gccgtaaaca taatctaaag ctaatgcttt tggaatcttt tcaccagact taccaataac
     1261 aatacctaat tcaccttcat aatctaattg atcagtaata tctttatgat ttggaatttt
     1321 tgcattatct cctgttaaag atgacgctgc ttttgtaaat acatataatc tttctacttc
     1381 atgatttaat tcgttcgcat gatctttgta atttctacca aaagcaatca cattattcgg
     1441 aggtgttact ggtggtaaga attcaatgtc attaaatgaa attttatagt cttcagcttt
     1501 gccgctatct tctgctgcta caactgcttt acgtacttgt tcttgaaaat ctaaagtatg
     1561 attttgttgt aaaccagcta acaatgtttt aggatggaaa tctccttctg caaagtcagc
     1621 aaatacttgt gttaaatccc atacagcatc ttcgcgtttt actttaacgc catatgaagt
     1681 tttgtcatta tacttgaatg ataagaattt cattcattct caactcctca tctttatctt
     1741 aattcacatt ataacttttt tcgttatcaa ataacaaata aataagtaag acaattttga
     1801 aaatgagttg tgttcattct gctacaagga ctttgcactt aatcgaaatt attttttatt
     1861 cttttgaaaa tcaaaatact acagttgcaa tgtaccaaat ttgaagaagt ataaataacc
     1921 tttaacttct ttattaagaa tcgtttgaag cgtattttga taatatttca tctgtatctt
     1981 atatttattt tttaattgtg taccaatttc ttcatctgtc atcccacggc gacgattaaa
     2041 tgcatcggtt ttatagtcta caaaataatg cacaccatct ttaacaaaga ttaagtcaat
     2101 cataccttga ataattgaga cgtcttcgtc tccttgtggc aattggtcaa ctaatgcttg
     2161 gttaactaca aacggtaatt cacgataaac ttgctctgct tcagcaataa tcgaatataa
     2221 ctcactattg ataaatgtca ttatttcatc catacggata tcttttttcg tatctgcttc
     2281 gataatatgt ttatcgatta atccatcgat atactgatgt aactcaactt cagatatgcg
     2341 ttcttttttg aatggtaaat gttgcatcac tgtatgcatt aacgtaccaa tttcattcgc
     2401 ttttcgttta ccttgttcac ttagaaattt aggtcgttca tacgttgaaa aaccgatacg
     2461 atattgcctt actcgttcgt aacttgtgcc actttcttca gtttcatatt gtcttttcaa
     2521 ttcagaaaca gattgttttg agggcttttt agtatcattt acatatggat attgataatc
     2581 aagttggtgt ttaatttgtg ctttaacatc ttcattacca ttttgcatag tttctaattg
     2641 attaaccgaa cgatattcat cattatctaa aatggtttct gtagacacat cttcaaagta
     2701 tataattgaa atatttacat tcggacgact actatcttca atttgtgcta tatctttttc
     2761 aaattttaaa tcatctggaa ttgacgcaga ttgatgttta gataaaatac tataaataag
     2821 atggaacgga tttggtgaag ttaatcgttc attgacagca atgtgctcac cagaaataga
     2881 caattgctct agttctagta atgatttatc atttttcact ctaccaatta aataaagttg
     2941 ttctttcgct cttgttaatg ctacatagac taatcgcatt tcttctgaca caagttcttt
     3001 ttcggcaaca gctttatatg caaccgaagc taaagatgga aatgccattt ctttatccac
     3061 atcaaaataa tccattccga gaccaaattg ctgatttaaa ataactggtt gtttcaaatc
     3121 acgtttatta aaatcttttg acaatccaga ataaatgaca aatggaaact ctagaccttt
     3181 actactatga attgtcatca ttctaacgac attatcgttt ggaccaacta cattttcctc
     3241 accaaaatct ttgcctcttt caatcaattc atcgataaaa cgaataaatt gatataaacc
     3301 tctaaaactt gaattctcaa actcgatagc tttattaaat aaaccataaa gatttgcacg
     3361 tcgtccacgt ccaccaataa gtccactaaa gtattgaata acataatgat cattataaaa
     3421 tttatcaatt aactgataca ccggatgatc tttactatat tgttggtaac tttgaatatc
     3481 tgataaaaac atttttaatt tatcaactaa aatagcatct gctgcttcgt cattaatgta
     3541 atttacaatc gattgataga agtagtcatc attcggactc aatattctaa tttgagctaa
     3601 ttcatcttct ttgaactgat aaataacgga acgcattaac ccaactaaat aaatatcttg
     3661 taatggatta tctatcgctc ttaaaaatga taatactaaa cggacttctg tttgttcaaa
     3721 gtatccttca cgactattaa catggaatgg aatatcttca tttttaaagg cttgttgtaa
     3781 attgcgagct tgtccaaagc tgcgttctag aataacgata tccttgtatg tcgcacttct
     3841 atagcttcct gttttcatat caaaaacttt ttgatgttct aagatatctt taacttgttc
     3901 tactataaaa tgcgcttctt gttcactacc agttaaatca ctatgttctt gatccgcttc
     3961 aacaagcact tttaagttta ctggatggtc cgattcatca tatggtgcac cataatacaa
     4021 ctgtgctgct tcatcatatc ttacttcacc gacttgttca tccatcatat gtttgaatat
     4081 atagtttgtc gttgacagta cttctttacg agaacggaag ttttgcgaca aatcaattcg
     4141 acgcccagtg ccatctccat ctaaagtaaa ccgttgatac ttttcaataa ataaacttgg
     4201 atcagcttgt ctaaatttat aaatggactg cttaacatct ccaaccataa ataaattacc
     4261 attatgttca tcacccgttt tgatgcaaga tagtattttc tcttgaactc ggttcgtatc
     4321 ttgatactca tcgaccaata tttcatgaaa atgttgacgg tatgattcgg caatttctga
     4381 aggcgaacca tcctcattag ttaaaatttg taatgcaaaa tgttcataat cagaaaaatc
     4441 cagaatattt ttgctacgct tttttcgatt gaattctgac ataacatctt tcacaatacg
     4501 cgcaaggtac tttactcgtg gcgccaattg ttgcatatca gctttcaaat catcagcttc
     4561 tcttgaaaag taatca
//
LOCUS       DAMZPP010000174         4570 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMZPP010000174 DAMZPP010000000
VERSION     DAMZPP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.175"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..383)
                     /locus_tag="POH73_002541"
     CDS             complement(<1..383)
                     /locus_tag="POH73_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDI6888451.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIVAQGASTDSNYVQQYQLPGH"
     gene            619..1374
                     /locus_tag="POH73_002542"
     CDS             619..1374
                     /locus_tag="POH73_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6888452.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLDRV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            complement(1476..2141)
                     /locus_tag="POH73_002543"
     CDS             complement(1476..2141)
                     /locus_tag="POH73_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6888453.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPVTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMNQNTHMN
                     QQPHMQQGHMQSSNHQMNQSNKKVLPAAGESMTSSILTASIAALLLVSGLFLAFRQRS
                     TNK"
     gene            complement(2510..3109)
                     /locus_tag="POH73_002544"
     CDS             complement(2510..3109)
                     /locus_tag="POH73_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDI6888454.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            complement(3377..>4570)
                     /locus_tag="POH73_002545"
     CDS             complement(3377..>4570)
                     /locus_tag="POH73_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDI6888455.1"
                     /translation="SQQFWDIFVSKLFLALTVFAMYMVVITIFIDDYYLIFLLQGIYI
                     IGAELDISWFYAGTEKFKIPSLSNIVASVIVLSVVVIFVKDQSDLSLYVFTIAIVTVL
                     NQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPNGQLNLYTSISCVVLGLVGTYQ
                     QVGIFSNAFNILTVAIIMINTFDLVMIPRITKMCIQQSHSLTKTLANNMNIQLILTIP
                     MVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLIIPLNMLISRQYLLIVNKIRLY
                     NASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFFLLIWRFIDITKINVKLNIVST
                     IQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIVVYLLLMMTMKNQYVWQILRHL
                     RHKTI"
BASE COUNT         1546 a          774 c          674 g         1576 t
ORIGIN      
        1 aaatgacctg gtaattgata ttgttgaacg taatttgaat cagtagaggc accttgtgca
       61 acaatcacat catataaatc aatgttttct tgcatcgcgc cacaagaacc aacgcgaatt
      121 aattttttac aaccaaatgt atgaattaat tcataagagt aaatgccaat agatggcata
      181 cccattcctg aacccatgac agaaactttt ttacctttat atgttccggt aaaaccaaac
      241 atatttcgca ctgtattgaa ctgttccaca tcgtccaaat atgtttctgc aatgaactta
      301 gctcttaacg gatctcctgg caatagaacc gtttctgcaa tttcgacgtc attcattggt
      361 ttaatgtgtg gtgttgattt cataaaatat tcctcccaat atttgtatag taatttgtct
      421 atttgtctaa ccatgtatat aataatattt tttaaaagcg ctttcaagca ttacattttg
      481 acaatattaa gaacatttta cagttatctc tactgatctt gcataaaacc aatgttcaag
      541 ttgtttctat gtttaatttt tataattttt ccaattcgag taaaaattat tgtatgatga
      601 gaatgattaa gtaactttat ggaggttgtc tacgtgacgc aaacacaacc aaaattttta
      661 acgatttata atacgttata taaagaaatt caaataggaa aatatccgag tggccatgca
      721 ttacctactg aaaaggaact ttgtgcgcgc tttgatgtca gtcgtatgac tttgcgccaa
      781 gctataaaat tgttagccga agatggcatc gtagagagta caagagggaa gggacacttt
      841 gttattccac aaacaaatgc ccaacacgct tcaagtatgg atcaatttaa gcatccactt
      901 gatcaaatat tattagatcg tgtatctatg tcatcaatta attatcgtgt tgatttagaa
      961 agtgaatata caaatcattt gtttgcaaac catccttctg ccgtcattgc aatggaacgc
     1021 tattatcaaa aaaaggacaa ccattctaag caagctgatg cattttgttt tacctttata
     1081 ccgttaaatg tcattgacac atttaaagtg aatacacaaa acgaagacga catgaagacg
     1141 ttcgttgagc aaacgattta tagcaatgca tatcaatcag atttaaaaat gtctattacc
     1201 aaagcgcccc acttcaaaaa tcatagtcac gtgtttgatg gtgatacaca ttgttggctg
     1261 attattgaaa cattgtacgc acaaacgccc tatccgatta tgattaataa gtggtatatt
     1321 ccacaggaaa tatctgaatt aacactgaca cgtataagac aatcggatta ttaaaattat
     1381 tgaaaaaaaa tcatgtgatg actgtttagc tattgtttaa tcgacaataa ctttcaggta
     1441 atcacatgat ttttctactt taaaacgtat gatgtttatt tatttgttga acgttgtcta
     1501 aatgctaaga ataacccaga tactaatagt agtgcggcaa tacttgcagt aagaatactt
     1561 gatgtcatac tttcaccagc agctggtaaa acttttttgt tactttggtt catttgatgg
     1621 tttgatgatt gcatatgacc ttgttgcatg tgtggctgtt ggttcatgtg tgtgttctga
     1681 ttcatatgac ctgttttatt ttgcaccatt tcactaccac catgattaac accatttgca
     1741 tctacattcg tttgataaaa attgccatct tttgttacag gtaacgcatg gccattcata
     1801 tctctaacag catgtggttt aaatacccct acattagttg taattatcac attgtattta
     1861 tcagcaatat tttgcgtcgc atcatacaat ttcatattat agtcttgcat attactgcca
     1921 gcgcttaatt ggggtaaagc tttctctaat tcagtatatt gtgcttgcgt cattccttgt
     1981 ggtactactc cgccataagg gcgatgattt tgtacatttt ggctttgttt attcgacaca
     2041 ttcattgaag ttgtgtcagc atgcgcattt tgaaaaggca tacttgagaa tactaaagtg
     2101 cttgttacaa ttaaagaacc tactgttgct aattttttca ttgtcttttc cccttatatt
     2161 acaatttgat tacatttata ttatcatatg attacaaaag aaatgcaaca aaatttttga
     2221 atcattacaa ttttttataa aaatttcact ttagattcac aataattact tattttgtca
     2281 atttatttaa tgtcaatatg ttgattaatc aatagtgttg tctgatgtat atagcattta
     2341 agtcatcgtt atagtcaaca ataataaggt atttcgagtt gaaatttatc ttattatttt
     2401 gccactttta cgcgctatcc cattacacaa aaacgaataa gtaaagatat taagtacaaa
     2461 aaaggtgaaa cacctaatag atgctccacc tcaattatat taagttatat tattttgctg
     2521 cttggtataa ttcatcaact tttttccagt taacaatatt ccaaaatgca gtcatatagt
     2581 ctggacgttt attttgatat ttcagatagt aggcatgctc ccaaacatca aatagtaaga
     2641 ttggtgtttt gccttctgtt aatggattat cttggtttgg cgttgtcaca atttctaatt
     2701 ttccatcatt aacaactaac caagtccaac ctgatccaaa taatgttgtt gctttatttg
     2761 caaattcatt tttaaattca tctaaagtgc cccactgcgc tttaatgtcg tctattacgc
     2821 cacctttttc ttcagaatta ggtgatagta tttcccagaa taatgaatgg ttaaaatgac
     2881 caccgccatt attacggact gacatcctca tcgcttccgg taccttgtct aagttagcaa
     2941 tcatatctgc tagtgattga tgctctaact ctgttccttc aactgttgcg tttaatttcg
     3001 tcacgtacgt attgtgatgt ttgtcgtgat gaaactccat tgttctttga tctatatatg
     3061 gttccaatgc atcatatgca tatggtaaat ttggtaattt aaatgccata agtatattcc
     3121 tccttttatg aatatacttt tataataatt aatttcgggt gtgttttgca ataattatta
     3181 tttatttgtt atatccaatt atataaaatt taaatattcg aaattgatat aatatttaat
     3241 attgaatgca aaaaggcatt taactgcttt tgtttcccgc ttttaaagag aattaaaaaa
     3301 gacaacttcc atttttcaat aagaaattgc cttttcttct tattcttaaa tttctaaagt
     3361 atagcgttac cgatacttaa attgttttat gtcgaagatg cctcaatatt tgccatacgt
     3421 attgattttt catagtcatc attaataaaa gataaactac tataccaatc gcaattaata
     3481 gcagcgtagc gtacattgta ggaggcaaat aatgattgac cacaccaagc acaataaaca
     3541 tcataacggc agcaatgaca cattgaatcg tacttacaat attcaacttc acattgattt
     3601 tagtaatatc aataaatcgc caaatgagca agaaaaactc tgtaattaaa cgcgcaatag
     3661 cagcaccgta aattccataa aaatatatca aaacaagaca taatactaaa ttcattaccg
     3721 caccaatagt gattgacgca ttatataatc ttattttatt cactattaat aaatattgcc
     3781 tgcttatcaa catatttaaa ggaatgatta atacaagtat cgctaaaatg gtcatcaatg
     3841 ggacagttga tgcgaattcc tcaccaaaga accataaata aaatgatggc ataattgcaa
     3901 tcaaaccaaa gaccataggt attgttaata tcaattgaat attcatatta ttagctaacg
     3961 ttttagttaa actatgtgat tgctggatac acattttagt aatacgcgga atcattacaa
     4021 gatcaaatgt attaatcatt ataattgcga ccgttaaaat attaaatgcg ttagaaaaga
     4081 taccaacttg ttggtatgtg cctactaaac caagaacaac gcaagaaata ctagtgtata
     4141 agttgagttg accatttggt aataaatatg ctaatgacga acgaaacaat tgccagacgt
     4201 gtatccaatt aaccgaaaca aagctaatgt atcgttttaa atagataaac aaaggtaatt
     4261 ggtttaatac tgtcacaata gcaatagtaa atacatacaa tgataaatct gattgatctt
     4321 tcacaaagat aacaacgaca cttaatacaa taacagacgc aacaatatta ctaaggctag
     4381 gtattttaaa cttttcagtt ccagcataaa accatgaaat atcgagttct gcacctataa
     4441 tatagattcc ttgtagtagg aaaataagat agtaatcatc tataaatata gtaattacga
     4501 ccatatacat cgcaaaaact gttaacgcta aaaataattt actgacaaag atatcccaaa
     4561 actgctgaga
//
LOCUS       DAMZPP010000175         4552 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMZPP010000175 DAMZPP010000000
VERSION     DAMZPP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.176"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..170)
                     /locus_tag="POH73_002546"
     CDS             complement(<1..170)
                     /locus_tag="POH73_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1811 family protein"
                     /protein_id="HDI6888456.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKK"
     gene            complement(148..966)
                     /gene="recX"
                     /locus_tag="POH73_002547"
     CDS             complement(148..966)
                     /gene="recX"
                     /locus_tag="POH73_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDI6888457.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEMFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            complement(1227..2036)
                     /gene="sgtB"
                     /locus_tag="POH73_002548"
     CDS             complement(1227..2036)
                     /gene="sgtB"
                     /locus_tag="POH73_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HDI6888458.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            complement(2377..2892)
                     /locus_tag="POH73_002549"
     CDS             complement(2377..2892)
                     /locus_tag="POH73_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDI6888459.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVF
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            complement(3022..3183)
                     /locus_tag="POH73_002550"
     CDS             complement(3022..3183)
                     /locus_tag="POH73_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888460.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            3407..>4552
                     /gene="yfkAB"
                     /locus_tag="POH73_002551"
     CDS             3407..>4552
                     /gene="yfkAB"
                     /locus_tag="POH73_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HDI6888461.1"
                     /translation="MNSVKLKQPVSIHNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHNRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNACTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRTAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQ"
BASE COUNT         1451 a          779 c          620 g         1702 t
ORIGIN      
        1 atcttttttc tgtctactaa gtaactttcg gcaacaatta ctttactttg ataaacgtca
       61 tattcattta aaataccatt catctcagct tttctcattt tctctttata aagttgtatt
      121 tcgtgtctta attctggctc agacatctca ctcaatttct tctgttccat cggcaatacc
      181 actttcttct aatttagctt taattttatc atatttatat ccttttctca taaggccttc
      241 gatagttttt gaaattaatt tttgttgcgt gtacttcttt cgatttttat tataaatttt
      301 ttctaaatct cgttgtaata aatcgtctaa aaccgcttca tcttgtgtaa aatccatttc
      361 attcaataca gcatgaatgg tttccatttc aaaccctttt tgaattaaag attgcattac
      421 tttttcttta accttatttt gtggcccctt ttttgtttta gatatttttt cagcaatttg
      481 gataatatca tcaagttcct gttgttctct ataaagttct gtaaacattt caatgatatt
      541 tggttctata ccaagttgat aaagtttttg ttgataaatt ttaggtcctt tatctgtcgt
      601 gcgaatcatt gtatttttta aactttccgc ataatcttga tggtcgatta acttttcgcg
      661 ataacaatat tcaatcactt cagaaatcgc ttgctctgat atctcttctt tttgtaaata
      721 ttgtataact tctttttcag ttctcttttt atatgataaa tattggattg ctttatttaa
      781 acctatgcga taatgatcat acttttgaat ctctgccatg tcagcagctt caagttgttg
      841 cccttttttt aaattaaatt tgactaatgt atcgatatct atacccattt caaattgttc
      901 atctaaaaaa agattaaaac gttctttgtt tttcttttga acttctattt tagtaatctt
      961 cggcactgga tcaccccgtt actttctcaa gttttaatac aatactcttt tattattcaa
     1021 taagccactt cctatagcaa atgtttaact ttaaatattt ttcgatgcta acaaaaaatc
     1081 acactatcat cttttaaaat gaaagtgtga ttacaagcaa atctgtaaaa tttattaagc
     1141 agaaacaatt caactttatc attatggcat ttcaattata ccttctacat tattgttcca
     1201 agtatcttac acatgaatgc aagtatttaa cgatttaatt gtgacatagc ctgttgatat
     1261 tgtgtttcat tgatataatt ttgttgcttc attttttcta agttcgtgct tacacgttgc
     1321 gtaaaattct cagacatatt attgatatta tatacgctag gtgcattgac tttactagct
     1381 aaaatagcgc tttgtaaaac tgtaatgtga gacattgttg tactattttt attcacggtt
     1441 gttccaaagt aatggtttgc tgcgccctca agcgtatatt gattatcccc aaagtaaata
     1501 ttatttaaat aaaagcttaa aatttcgttc ttattatatt gtttttcaac tcgatgagct
     1561 acaaataatt cttttacttt tctagtaaat gaacgatcat tatcataaaa ataatttttg
     1621 acaacttgtt gtgtaatggt actaccacct tgcacatctc tgtcgctaat cgttgaaaat
     1681 aaagctctag ttgtaccttt caaatcaaat ccatgatgat tgtagaatcg ttcatcttcc
     1741 attgaaataa aggcaccttt aacatactct ggcatgttat cagctgacac aaaactactt
     1801 ttattttcaa tttttcttag ttcatccaca ttatcgcgtg tagataaaaa atacatgata
     1861 ccaataaaca atgcgataat gattagaatg gttaataata ttttcaatag tattcgttta
     1921 ctttttttct ttttcggcgg tttgccaact ggttgataat acgtattata gtgaggttcg
     1981 tgtttcatat gctcaaaatg ttcatttgag tttgagtacc tatcgcttct tttcatgcgt
     2041 ttgctccttc ttttaaaact cacttagtat ataccttgag tttaccagta ctatcacaaa
     2101 taggctacac tttttgggaa aatcagtcca agggcttaca atcgtatacg ccatcatact
     2161 tacttttttg ttttttgaaa agattataga taaatcattg cgattttaaa tattaatcat
     2221 gtcaaatatt gttatatttt ataaaaataa aagaccatcc ctattaaatg ccaatagaga
     2281 cgacctttta tttgttattc attttttaaa actaaaatcc atatttcatt tcaaacgaaa
     2341 atatataaat tttaacaatc gataaccaca atacttctat tgtaattgtt taacaatttc
     2401 tcgattaaaa tcatctaaat cgtctggtac tcgacttgtt acaatattgt tgtctacaac
     2461 tactgactca tcaactacat gtgcgcctgc atttgataaa tctttgcgta catttaatac
     2521 tgctgttaac gtacgacctt ttaaatcgtc tgtatctatt agtatttgtg gcccatgaca
     2581 aatggcaaat gttggtacat catttttagt aaagtattta gcaaatgtgc catatcgacc
     2641 ttctgtatct ccacgtaaat gatctggtga aaaccctcca ggaattaata atgcatcata
     2701 atcttctggt ttagcttctg caatgcctac atcgacagta actttttcac cgtgtttacc
     2761 aaaaacttca ctatttgcag tatctccaat cactacagta ttaaagccag cattctctaa
     2821 tgcctcttta ggacttgaat attctatatc ttcaaattcg tttgctagaa taattgctac
     2881 ttttttagtc attgaaaatc acctttctat atatcattga tataaatact atagacaagt
     2941 aaatcagtga ttaaacatac aagatataaa aacattaagc gactgtcgcg atatctaact
     3001 ctaacacatc ttatgtggca tttacttaga tactaattta accttttctt caagctgatc
     3061 taacaatcca atccattcat ctatatcttc aacacgtact tcatcaggat ttacatgatc
     3121 gatatcctca ataaacttat ttaaacgcgc ttttatctgt tcgattgttt gctgttcatt
     3181 cataaaaagt taactccttt tattttgttt tctttttcat tattatccta acagaaattg
     3241 cgttaaagcg atataatctt agctatattt atgacattca aattattttg acttttaaaa
     3301 atcccctttt caattaacta aaattaagag ataatttgtt acgagtgata atacgaagtg
     3361 gtatcatacc gatatgaacc aaatagaaag aaggaagttt aagacgatga atagcgtcaa
     3421 attgaagcaa cctgttagca ttcacaatga tccatgggaa gcatataacg atgttaaaga
     3481 acatggccaa ttaactttaa gtaacatcga atttacaact acaaatcttt gtaatatgcg
     3541 ttgtagccac tgtgcagttg gttatacttt acaaactgtc gaccccgagc ctttagatat
     3601 ggacttaatt tatcgtagac ttgatgaaat tccaaatctg cgaacgatgt caattacagg
     3661 tggcgaacca atgttttcta aaaagtctat tagaaatgtt gttaaacctc tattaaagta
     3721 tgcacataat cgaggtatat atacacaaat gaattcaaac ctaacattgc ctcaagatcg
     3781 ttatttagat attgctgaat atatcgatgt tatgcatatc tcacataact ggggaacaac
     3841 tgatgaattc gcaaatgttg gctttggcgc aatgaagaag caaccaccgt taaaagctaa
     3901 gttaaaatta tatgaacaaa tgatttcgaa tgcatgtaca ttatcagaac aaggaatgtt
     3961 tgtatctgcg gaaacaatgc tcaatcaaag tacgctacca catttacgaa aaatacatca
     4021 agaagtcgtt catgatatga aatgtagcag acacgagatt caccctatgt atccagctga
     4081 ctttgcaagt caattaaatg tgttaactct agcggaaatg aaaaagacaa ttcatgatat
     4141 attggatttc agagatgaag atatttggat gttatttggt actttgcctg tgtttccatg
     4201 cttaaaagat gatgaagatc aaaagttact atcacgttta agaactgcta aaaatgtaac
     4261 gactagaaat gacccggatg gccgtagtcg tttaaatgtc aatgtattta caggtaatgt
     4321 aatcgtaact gatttcggag atgaaacagg tacaatttcg aatatacaaa aagataaatt
     4381 aacagatgta tttgataaat ggttatcctc tgatcttgct aaatcattaa attgtcattg
     4441 ttccgagttt agttgtttag gaccaaatgt tcttgttaaa aatatgtact atccgaatat
     4501 ggattttaaa gataatgagc gtcatatgca caaacaacca caaattatac aa
//
LOCUS       DAMZPP010000176         4537 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMZPP010000176 DAMZPP010000000
VERSION     DAMZPP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.177"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..1148
                     /locus_tag="POH73_002552"
     CDS             <1..1148
                     /locus_tag="POH73_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDI6888462.1"
                     /translation="TIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNSYTN
                     RIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHASKQY
                     QLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDKQNV
                     NHIFEKEHDQVVYDKTDHIVGSGITAAHLALKLLNHDDDKKIHLWLNKDIEIHDFDAD
                     PGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHKTPI
                     TQISDGLINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVISEK
                     LEWIPNLYVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(1204..1578)
                     /locus_tag="POH73_002553"
     CDS             complement(1204..1578)
                     /locus_tag="POH73_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDI6888463.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVIATAEIIHQGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(1597..2913)
                     /locus_tag="POH73_002554"
     CDS             complement(1597..2913)
                     /locus_tag="POH73_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDI6888464.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(3324..>4537)
                     /locus_tag="POH73_002555"
     CDS             complement(3324..>4537)
                     /locus_tag="POH73_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HDI6888465.1"
                     /translation="KIWGHLFQYIKSEHIEDTYLLMDSFISKYDQLSDVLMACGIQSE
                     RATYEFDHYKSSKKAPFKTNLNLISESLIELDFIHEGISIGQSINLARDFSNMPPNVL
                     TPQTFAEDIVNHFKNTKVKVEVKDYDTLVSEGFGLLQAVGKGSKHKPRLVTITYNGKD
                     KDEAPIALVGKGITYDSGGYSIKTKNGMATMKYDMCGAANVVGIIEAASRLQLPVNIV
                     GVLACAENMINEASMKPDDVFTALSGETVEVMNTDAEGRLVLADAVYYANQYQPSVIM
                     DFATLTGAAIVALGDDKAAAFESNSKVILNDILQISSKVDEMVFELPITATERASIKH
                     SDIADLVNHTNGQGKALFAASFVTHFSGQTPHIHFDIAGPATTNKASYNGPKGPTGFM
                     IPTIVQWLKQQ"
BASE COUNT         1540 a          857 c          665 g         1475 t
ORIGIN      
        1 ggacaattat cggcggtggc atacagggaa ctgcaatcgc acaaaaacta ttatcaagcg
       61 gattaacaac agaccgatta acaatcattg acccacatga aactttttgc caaaggttta
      121 actcatatac aaatcgaata gaaatgcctt atttaagatc accgattgta catcacgtac
      181 atccacaacc gttccatcta aaacaattcg ctaaacagca ccaatataca aatgcttttt
      241 atggtcctta tcagcgacct gaattgacaa tgtttatgga tcatattgca catgcttcta
      301 aacaatatca attagaggat tgcttggttc aaggtttagt tcaaacttta gataaacaag
      361 aagacaaatg gcatatcaag ttagaagatg gacaaattat cactacagat tgcgtcgtta
      421 ttgcaatagg cagtacaaat attccgttta tgcctgacat tttaaaagac aaacagaatg
      481 taaatcatat cttcgagaaa gaacatgatc aagtagtata tgataagacc gatcatatcg
      541 ttggtagtgg tattactgct gcacatcttg cacttaaatt gttaaatcat gatgacgata
      601 aaaagattca tttatggcta aataaagata ttgaaataca tgactttgat gctgatcctg
      661 gttggttagg tccgaaaaat atgtcttcat ttttaagtac taaaagtatg cctgaaagaa
      721 atgccattgt acaacgtgaa cgtcataaag gatcaatgcc tcacgaactg tacttacgcc
      781 ttaaaaaaca tattaaaaat ggtcgtataa atgtgcataa aacacctatc actcaaatta
      841 gtgatggttt aattaacact gaaaatgatt ctgttccata tcaacagatt atggttgcaa
      901 ctggttttga acaagatttt atgtcacaac cacttattaa gcaattaata caaaattatg
      961 atgcacctat caacgaatgt aattaccctg ttatttccga aaaattagaa tggataccaa
     1021 atctatatgt cgcaggatgc tttgcagact tagaattagg accatttggt agaaatgtta
     1081 tgggtggccg taaagctgcc gaacgcattg aacaagcatt tctaaaatta caacaatata
     1141 gcgcataaaa aaactgctag ccgtagttat gaatatctca taacttttca gctagcagtt
     1201 cttttatttt aaaggtttaa tagcaactgt accacgcata actgtaatta attgttcttt
     1261 gtcattctta atttttatat cccatacatg tgttgactta ccttgatgaa taatttcagc
     1321 tgtcgcaata acacgaccat ctttagcaga atgaatatga ttggcattca tctctaaacc
     1381 taatggaata aatttggttg tatcaattag attagcagat cctaatgaac atgctgtttc
     1441 acctaaagca atcgaagcac cgccatgtaa atatccaaat ggttgtttta ctttatcagt
     1501 aacaggcata gaaataacaa ctaaaccatc ttcctggtga tctattgaca tctcaaatgt
     1561 ctctaataaa tgagtcatat tttcacctct ttatgcttat agtaccatag cagcaatagt
     1621 accgaaaatc attaaaggaa tattataaaa caagaagttt ggtacacatg tatcgcgtat
     1681 atgatcatgt tggccatcaa catttaatcc cgcagttggt cctaatgttg aatcacttgc
     1741 aggtgaacct gagtcaccta atgcactcgc tgttccaatc aatgcgatta atgccattgt
     1801 atctagtcca attgacgctc caaaaggaat gaataatgat gcgataatag gaattgttgc
     1861 aaatgatgat ccaataccta aagtgacaat taaacctatc acatacatca tgataatgct
     1921 aaataattta ttatcaccag taatacttgt taaagtttta actaattcat ctatatcacc
     1981 agtagcattc attacaccag caaatccatt tgctgttaaa ataactacac caatataagc
     2041 cataatttta ataccttcaa caaacttagc atctaattca taccaattat atgcacgtga
     2101 aataaagaat acgagtaccc cggccagtgc accaaaaatc attgaatctg taaacgtttg
     2161 tactaaaaat gtagctagta ttgccacaat tgttactatt aagatatatg gttttaactc
     2221 tgtaacattg tcactatctg aaattttacg tgtttcatat tcacgtggtt tacgatatac
     2281 atataaaccg ataagtaagc caacaatata ccccattgaa ggaataagca ttgctttcca
     2341 aatcatatta aactcaattg ggtgattcgc ctttgcaaag ccactttgaa taatttgctg
     2401 gaaaatttga ccgaatccat atggtaataa cacatacggg aaacataaac caaaaccgat
     2461 aatcaaaccg attaaacgtc tatctatttt taagtcatta aacagactta ataatggtgg
     2521 gatgacaatt ggaatgaatg caatatgtac aggaattaag ttttgactca tgatactcat
     2581 agctaataat gcaatgatta ttgtcacttt cactttaact cttgaccatc gactattttc
     2641 agcgtgaatt gcatttatta tttttcctac taaatagtct gtgataccac tgtatgaaat
     2701 taatgctgca aatccaccta ataaggcata gcttaatgct acctcagcac catcgactat
     2761 atttttccca aatacattta taactttttc aatgctcatg cctgaaatta agccacctac
     2821 tagcgcactg ataaataagc ttataactac gtttaatcga cataaacata gcacaatcat
     2881 taatattact gctattacta ctgcatttat catatgacaa ccacccttta tcactctatc
     2941 gaactaaagc gtttatagac tacatagtat cagtagcttt aacacctgtc aatagcaaac
     3001 tataccaata atctttattt tttagttaat tgtctttctt aaaataattt tcgctttcat
     3061 taaattaaac aattttacaa gtctggaaca ccaatcaaaa tcctaagttc tataatgtta
     3121 tattagtagt cgttgactga atgaacatat gcttataata agtttttgca atgctaatca
     3181 agttgattta tgctcacaag gatatgcgat ttatattttc ttacaacaat gaaaatgcct
     3241 gatacaatgc gatcctttat actttcaaga gttaaattag aaatgtataa caagacacaa
     3301 gttgtatcag gcaaataatt aaattattgt tgttttaacc attgtactat cgtcggaatc
     3361 ataaatcctg ttggcccttt tggaccatta tatgaagctt tattagtcgt tgctggacct
     3421 gcaatatcga aatgaatgtg aggtgtttga ccactaaaat gtgttacaaa acttgccgca
     3481 aatagcgctt taccttgtcc attcgtatgg ttaactaaat cagcgatatc actgtgttta
     3541 atacttgcac gttcggttgc agtaatcggt aattcaaata ccatttcatc gactttagaa
     3601 cttatttgta atatgtcgtt taatatcact ttactattcg attcaaatgc agcagcttta
     3661 tcatcgccaa gtgcaacgat ggctgcaccc gttaatgtag caaagtccat aatcacacta
     3721 ggctgatatt gatttgcata atacacagca tctgcaagga ctaatctacc ttcagcgtct
     3781 gtattcatta cttctacagt ttcaccactt aacgctgtaa atacatcatc tggcttcatt
     3841 gatgcttcat ttatcatatt ttcagcacaa gcaagcactc cgacaatatt tacaggcagt
     3901 tgtaaacgac tagccgcttc aatgatacca acgacattcg cagcgccaca catgtcatat
     3961 ttcattgtag ccatgccatt cttcgtttta atactataac caccagaatc atacgttata
     4021 cctttaccaa ctaaggcaat tggtgcttca tctttgtctt tgccattata tgtgatggtt
     4081 actaatctcg gtttatgctt actaccttta cctaccgctt gtaaaagtcc aaatccttca
     4141 gaaactaaag tgtcataatc tttaacctct actttgacct ttgtattttt aaaatgatta
     4201 acaatatctt ctgcaaatgt ttgtggtgtt aatacattcg gtggcatatt actaaagtct
     4261 cttgccaaat taatggactg gccaatactg ataccttcat gaataaaatc taattcaatt
     4321 aatgattcac taataaggtt taaatttgtc ttaaacggtg ccttcttact tgatttataa
     4381 tgatcgaatt catatgttgc acgctcactt tgaataccgc atgccattaa tacatctgat
     4441 aactgatcat attttgaaat aaatgaatcc ataagtaaat acgtatcttc aatgtgttct
     4501 gactttatgt attggaaaag atgtccccat attttca
//
LOCUS       DAMZPP010000177         4504 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMZPP010000177 DAMZPP010000000
VERSION     DAMZPP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.178"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..242
                     /locus_tag="POH73_002556"
     CDS             <1..242
                     /locus_tag="POH73_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDI6888466.1"
                     /translation="TGQNGAFHPELKRKIESIKAIANVPVVAGFGIRSPQHVADIKEV
                     ADGIVIGSEIVKRFKSNTREEIIKYLQSIQQTLNN"
     gene            564..1826
                     /gene="femA"
                     /locus_tag="POH73_002557"
     CDS             564..1826
                     /gene="femA"
                     /locus_tag="POH73_002557"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HDI6888467.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRYLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKERVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            1845..3104
                     /gene="femB"
                     /locus_tag="POH73_002558"
     CDS             1845..3104
                     /gene="femB"
                     /locus_tag="POH73_002558"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HDI6888468.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
     gene            3767..4504
                     /locus_tag="POH73_002559"
                     /pseudo
     CDS             3767..4504
                     /locus_tag="POH73_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
BASE COUNT         1681 a          601 c          764 g         1458 t
ORIGIN      
        1 caacaggaca aaacggtgcg tttcacccag aattaaaacg aaaaattgag tcaattaaag
       61 cgatagccaa tgtgccagtt gtcgcaggat ttggtataag atctccacaa catgttgcag
      121 atataaaaga ggttgcagat ggtatcgtta ttggtagtga aatagtaaaa cgatttaaat
      181 ctaacacgcg tgaagaaatc attaaatatt tacaatctat ccagcaaaca ttgaataatt
      241 aagtttactt gatttaaaaa aattagtcga atactgtttg aaaaagtgaa aaacggtgaa
      301 ttataaaatt gaatagaaat gatacaaaag taatatgagc aaacccaaac gttcatatta
      361 ctttttttga aattgtatgc aaaaatctaa atattactat aaaagtatac gcaattaaag
      421 cgtttatgtt ttagttttaa cattaactat tgtatactta tttagattag atttattatt
      481 tttgacattt gcagagggga aatagaaaaa ctgcaaatac ggaaatgaaa ttaattaacg
      541 agagacaaat aggagtaatg ataatgaagt ttacaaattt aacagctaaa gagtttggtg
      601 cctttacaga tagcatgcca tacagtcatt tcacgcaaac tgttggccac tatgagttaa
      661 agcttgctga aggttatgaa acacatttag tgggaataaa aaacaataat aacgaggtta
      721 ttgcagcttg cttacttact gctgtacctg ttatgaaagt gttcaagtat ttttattcaa
      781 atcgcggtcc agtgatcgat tatgaaaatc aagaactcgt acactttttc tttaatgaat
      841 tatcaaaata tgttaaaaaa catcgttgtc tatacctaca tatcgatcca tatttaccat
      901 atcaatactt gaatcatgat ggcgagatta caggtaatgc tggtaatgat tggttctttg
      961 ataaaatgag taacttagga tttgaacata ctggattcca taaaggattt gatcctgtgc
     1021 tacaaattcg ttatcactca gtgttagatt taaaagataa aacagcagat gacatcatta
     1081 aaaatatgga tggacttaga aaaagaaaca cgaaaaaagt taaaaagaat ggtgttaaag
     1141 taagatattt atctgaagaa gaactaccaa tttttagatc atttatggaa gatacgtcag
     1201 aatcaaaagc ttttgctgat cgtgatgaca agttttatta caatcgctta aaatattaca
     1261 aagagcgtgt gttagtgcct ttagcgtata tcaattttga tgaatatatt aaagaactaa
     1321 atgaagagcg tgatatttta aataaagatt taaataaagc attaaaggat attgaaaaac
     1381 gtcctgaaaa taaaaaagca cataacaagc gagataactt acaacaacaa cttgatgcaa
     1441 atgagcaaaa gattgaagaa ggtaaacgtc ttcaagaaga acatggtaat gaattaccta
     1501 tctctgctgg tttcttcttt atcaatccat ttgaagttgt ttattatgct ggtggtacat
     1561 caaatgcatt ccgtcatttt gccggaagtt atgcagtgca atgggaaatg attaattatg
     1621 cattaaatca tggcattgac cgttataatt tctatggtgt tagtggtaaa tttacagaag
     1681 atgctgaaga tgctggtgta gttaaattca aaaaaggtta caatgctgaa attattgaat
     1741 atgttggtga ctttattaaa ccaattaata aacctgttta cgcagcatat accgcactta
     1801 aaaaagttaa agacagaatt ttttaggaag ggaattatca aaacatgaaa tttacagagt
     1861 taactgttac cgaatttgac aactttgtac aaaatccatc attggaaagt cattatttcc
     1921 aagtaaaaga aaatatagtt acccgtgaga atgatggctt tgaagtagtt ttattaggta
     1981 ttaaagacga caataacaaa gtaattgcag caagcctttt ctctaaaatt cctactatgg
     2041 gaagttatgt ttactattcg aatcgtggtc cagtaatgga tttttcagat ttaggattag
     2101 ttgattatta tttaaaagag ttagataaat atttacagca acatcaatgt ttatatgtta
     2161 aattagatcc gtattggtta tatcatctat atgataaaga tatcgtgcca tttgaaggtc
     2221 gcgagaaaaa tgatgcctta gtaaacttgt ttaaatcgca tggttacgag catcatggct
     2281 ttacaactga gtatgataca tcgagccaag tacgatggat gggcgtatta aaccttgaag
     2341 gtaaaacacc tgaaacattg aaaaagacat ttgatagtca acgtaaacgt aatattaata
     2401 aagcgataaa ctatggtgtt aaagtaagat tccttgaacg tgatgagttc aatctattcc
     2461 tagatttata tcgtgaaact gaagagcgtg ctggatttgt atcaaaaaca gatgattatt
     2521 tttataactt tattgacaca tatggagata aagtattagt accattagca tatattgacc
     2581 ttgatgaata tgtgttaaag ttgcaacagg aattgaatga caaagaaaat cgtcgtgatc
     2641 aaatgatggc gaaagaaaac aaatcagata aacaaatgaa gaaaattgca gaattagata
     2701 agcaaattga tcatgatcag catgaattat tgaatgcaag tgaattgagc aaaacggacg
     2761 gcccaattct aaaccttgct tctggcgttt attttgcaaa tgcatatgaa gtgaattatt
     2821 tctctggtgg ttcatcagaa aaatacaatc aatttatggg accttacatg atgcattggt
     2881 ttatgattaa ctattgcttc gataatggtt atgatcgtta taatttctat ggtttatcag
     2941 gtgattttac ggaaaacagt gaagattatg gcgtataccg ctttaaacgt ggatttaatg
     3001 tacaaatcga agaattaata ggggatttct ataaaccaat tcataaagtg aaatattggt
     3061 tattcacaac attggataaa ttacgtaaaa aattaaagaa atagatgtaa atgatgttag
     3121 ggcttatagt tattgatact ataggctctt ttttatgctt tttaataaag cctctaatga
     3181 tttattaaat gacaagttta acaagtgatt gcaataaata taaatacgaa ttgcacaatc
     3241 tattttcaaa tgattaagtt gagtatacgt tttaattcat tcaatatgac tcaaaacaag
     3301 ccaatatgta ttatattttt ggtgaataaa ccttagcata acaatttata tctgatttta
     3361 tatgtgtgtt tgcaagtgcg tgatatcaaa tgtcatatgt aatttataaa atgcatgaga
     3421 aatgattgct gtaacaaatt ctagcaaagt aatcagtttg tatattagaa ataaaaatga
     3481 tgatagatat attatcgata catagattta atatgcacca aaacgtgagt attggtttaa
     3541 tgaaacatag catgaatgta aatttagttt actgacataa acacgtcaca aacatacagt
     3601 tatataagtc cccttatgga atttcatttc tagtttacat aatatatatt ataggaagtt
     3661 atatgtgtct aacgcaaaag gtaccctaca tcataatcat tatctaatat cgtcacataa
     3721 cttacttatg ctataatcat ggtattatat tgtttggagt gatttgatga aatttgtctt
     3781 tgatattgac ggtacacttt gttttgacgg ccgattaatt gaccagacta ttattgatac
     3841 attgttacaa ttacaacatg ctggtcatga acttatattt gcatcagcac gtccgattcg
     3901 tgatttgttg ccagttttac catcagtatt tttatcagca cacattaatt ggcgcaaatg
     3961 gtgctatgat ttcacagcaa tcaatgattt ctgttatcaa accaattcat acagatacat
     4021 atcatcatat cttaaaaata attcaaaagt atgagttaga ttatattatt gatgatgatt
     4081 ggaattatgc tgcacaactt gacgctgaga acgctatttt tgagcgttta gatccacata
     4141 agctggccag ttgtattgat gttgcaaata tcgacacacc gatcaagatt attttattaa
     4201 atatagaacc gacacaaatt acaactatat tagatgagct caataaatac catcaagaat
     4261 tggaaatgat tcaccattca aatgaatata acattgatat aacagcgcaa aatattaata
     4321 aatatactgc attacaatat atatttgatg cagatgttaa atatatagca tttggtaatg
     4381 atcacaatga tattgtcatg ttacaacatg ctagtaatgg ctatattata ggaccatcag
     4441 aagcatacac acacgcaata ttgaaacttg ataaaatcaa acacatcaat aataatacac
     4501 aagc
//
LOCUS       DAMZPP010000178         4501 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMZPP010000178 DAMZPP010000000
VERSION     DAMZPP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.179"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..2735)
                     /locus_tag="POH73_002560"
     CDS             complement(<1..2735)
                     /locus_tag="POH73_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDI6888469.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFATFKSRVEKSIA
                     DWDIKLEHTFYVSKFDHSENELGALSTYLVSLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGLFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLVDRQETTYQVATDNAKDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDISFSTIEDFINSDLEKLKSHLNKNQ
                     LAFVHAVEKHYNLYVSMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQAFKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDHSYVEMITEFETNKADISRRQHRLTV"
     gene            3039..4379
                     /locus_tag="POH73_002561"
     CDS             3039..4379
                     /locus_tag="POH73_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDI6888470.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVERQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWTIFMILSGTYLL
                     IKKHLISNKSKALHWQKWHAMIGIIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGDL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKSGIKKPFSIVYPSDKIGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGIVNKIINLFVCIALLVAIGMGFVSWINRTKN
                     TAVKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYIKDRRAKQ"
BASE COUNT         1416 a          730 c          687 g         1668 t
ORIGIN      
        1 actgttaacc tatgttggcg acgtgaaata tcagctttat ttgtttcaaa ttctgtaatc
       61 atctctacat aagaatgatc catttgttga agctgatgaa tcatttgttg ttctaaaatt
      121 gatttagatt caacatcaac aaattgttgt atgctttgtt ttaattgatc aatgccctta
      181 tcttcagctt gcaatgcatt tcgacttgat acagcaaaaa tgtctgattg taagtgtact
      241 tgtcttaatg catctgatac atatgtttca accgcttcaa gatcatcttg actttctgct
      301 aaatcagcag cattaattac cattttaaat gcttggtttt cattcaactg gttcatatct
      361 ttcatgtgtt ctataaacgc cttgtcatta tcagtaaacg aatgattaaa ataacttaca
      421 tacaagatta agtctgaaga agttaaaatt tgctcggttt catttgtatg cctttggtta
      481 tttgagtgta gccctaatga atcaacaatt attttacctt ttaaccaatc atgcattaat
      541 gcaatgtgta ctgttttaac aaatgttgcg tattcatctt ctgcactcca ctttttcaat
      601 tcctgttgat taatggcatg tttttctcca ttttccaaca tactgacata caaattataa
      661 tgtttttcaa ctgcatgaac aaaagcgagt tgatttttat ttaaatgtga ttttaacttt
      721 tctaaatctg aattaataaa gtcttctata gttgaaaaag atatatcttg gtattcaact
      781 actgcattaa tttcatctaa taattgcgat tgtgatttta gcgttatata actctcgtct
      841 ccataagata tttctgtagt agctgctgta gtaggatttg gagaactaac taaaatatgc
      901 tcgcctaata atgcatttat caaactactt ttacccgcac taaacgttcc aaatacacct
      961 atttttatta atttattatc tatacgtgtt aatgtttcgt ggatatcctg ctttgtacgc
     1021 ttgaacaaag gcacatccga aattatatca agtgcttttt gaatatctat agacatattt
     1081 gttgtagctg tattctgatt tagctgctga ttatcacgat tatccttagc attatcagta
     1141 gccacttgat atgttgtctc ttgtcgatct actaatttat ctagagattc atctaaatga
     1201 atatagtaat gacgataatt cttagtcgtc aatgactgac gcagctcatt taattctgta
     1261 taacgttgat attcttttaa atcatcactt tcttctgttg gtaattcatc tgcctgcaca
     1321 ttttctatta tttctttaaa aattggtgtt gattgttgtt caacatattt cttaatggct
     1381 ttaaccactt cgtctgaaaa tgtaagtaca taagtattgc tgattgatgt ttgtggttga
     1441 tataaacctt caatcatttc cggcttaacg tcataatgct gatttaatac tttatctgaa
     1501 gcttcttttt tattgataaa acgcgtaaca aatgacatat cttctcgcat tggttgacga
     1561 atttgttgat taacatgttc ttgtaacgca tctgtcgctg ttaataatcg ctgttgttga
     1621 atttgaagct ttttcttctt tttattaaaa aggccaccaa cattaaagtc ttgagacata
     1681 ctttccaaat aacttcgtaa catttctctc atattatgcg gcataatgta tgcattttct
     1741 aaaatatttt tacgcttatt ctttaaaaat gccattaatt catctggatt atttaaaagt
     1801 tgcgcctctt cactaattgc ttgatgttgt tgactattta aaaatgcttg ttcaaactct
     1861 gcttcttcga tacccaaatc ttctagtact tcctgaattt cagactgaat gtaatctagc
     1921 tgagcttcgg taatatattc aaccgttctt gatgtataat cctctattgt ttctctatgt
     1981 tgatctaatg aaactagata agttgataat gctccaagtt cattttcaga gtgatcaaat
     2041 ttcgatacat aaaaggtgtg ttctaattta atgtcccaat ctgcaattga tttttcaact
     2101 cgagatttaa acgtagcgaa tgacaattca tcgtcttgat gcttgtcaat ttgattaatg
     2161 ataaacacaa caggtattcc aacatcattt atatgcttca taaacttaaa gttaagttca
     2221 gattgaacgt ggttatagtc aaccgtataa aatatcatat tacttgtata catatattgt
     2281 tctgttattg actgatgtga tgcaacattt gaatcaacac ctggtgtatc ttgcaacgta
     2341 aacccatttt caaatttagc tgattgaaaa ttaatttcta cagattcaac gtcgacattt
     2401 tggcgattca tttcccttac ttcatcataa ttagataatt tggcatacgt ttgattcggc
     2461 aaattagcaa taatatcgtg attgtctgaa actgacacga tagcagtatt acttgtcgtt
     2521 ggtacaggtg aacttggtaa gatatcttgt tcaattaata aatttatcag tgtcgattta
     2581 cctgcagaaa aatgtccaac gaacgaacat gtatattgct gcaaatatac tttcttaatt
     2641 acttggttaa ttgtatgtaa aagtgcttca tttcgcgact tttcaacttc tttttttaat
     2701 ttatataaaa gatctaattg ttctttatta atcatgttac tttccccttt ggttcgttca
     2761 catagaaatg cttgcttgtt aaaacgaatt catacaatat cgatgtccat ttaaacaaag
     2821 gtcgttgaat ggctcgcatt gtattatgtt taaattttat catagctgct tgataaaaca
     2881 tatgaataat caattttctt caaacattcc aacttaaaag ttcatttttc taaaattcac
     2941 aaatcattgt taaatttttc tagaaaataa atattgtcac agtttgttca cacacgtcac
     3001 agaattgaca ccgtataata gccaattggg agggaaatat gaaaaataca tttaatccat
     3061 tacaaagatt acatttctac gcagcaatat ttattgctcc actgttaatc actttaacca
     3121 tttcgggcat tggttatttg ttctttccag aagttgaaaa taatatttat aagaatgagt
     3181 tttttggtga cagtgatgtc aaaacgcatc aaacattaaa tgatgcagta catcaagttg
     3241 aacgacaata tgaaggattc tttgtaagta aagttagcat acttgatgaa ccatataaca
     3301 aacgaattac actgagtgat atggcaggaa atcagcgtta cgtctttcta gatcataaca
     3361 atcaatttgt tgcagatcaa aatgcgaaac atacgtattc taatgtgatg cgaagtatac
     3421 atagttcttt gtttactgaa aatactatta ttaattattt agttgagtta accgcatgtt
     3481 ggacgatatt catgatttta tctggtactt atttactcat taagaagcat ttaatttcta
     3541 acaaaagtaa ggcacttcat tggcaaaagt ggcacgcaat gattggaatt atcattgcaa
     3601 ttccagtatt tgtattagtc ttaactggat tgccatggtc tggttttatg ggcagtaaaa
     3661 ttgccggtat gatggacaca aacggtgacc ttggtcaagg tgaattagcg attaatccac
     3721 ctaaatcaga tttgaatgaa ttaccttggg ctacacgtaa aaataaacag ccagcttcat
     3781 ccgaaaaagg ttcaagtggt catcatggta atgcagcaat gcctcaaact aaattagatt
     3841 atcaaatatc tattgataag gtcgttgaac aggcgcaaaa atctggtatt aaaaagccgt
     3901 tttcaatcgt atatccaagt gataaaattg gcacctttat tgtatctaat actagtaatt
     3961 caggtgttac tgggctagat gtatctcctt acaaggaaca aacactttat tttgatcaat
     4021 atagcggtaa aaagctaggt acgattaaat atgatgacta cggtattatt gctaaatggt
     4081 ttacgtgggg cattccgctt cacgaaggtc atttattcgg tattgtaaac aaaatcatta
     4141 atttatttgt atgtatcgct ttattagtag ccattggtat gggatttgtc tcttggataa
     4201 atcgtacaaa aaatactgca gtaaaagtac cacatcgcgt aaaaaaacca gcatctatat
     4261 cactcatcat atgtttaatt gtattaggac tattaatgcc attatttgga ttatcactta
     4321 tcctagtatt tataattgaa ttaatattat atattaaaga tcgtcgtgct aaacaataat
     4381 gcacttaaag ttttgaactg acgaaattta caaaatgatt ctctaattac ttaaaacagg
     4441 ttctataata aatcgaactg atggagcttg ggagataaat caatgttcta gggtctataa
     4501 t
//
LOCUS       DAMZPP010000179         4422 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMZPP010000179 DAMZPP010000000
VERSION     DAMZPP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.180"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            127..786
                     /locus_tag="POH73_002562"
     CDS             127..786
                     /locus_tag="POH73_002562"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDI6888471.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKTIEEVTDEILAMVSQI
                     K"
     gene            817..933
                     /locus_tag="POH73_002563"
     CDS             817..933
                     /locus_tag="POH73_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888472.1"
                     /translation="MQRLMHIQIECADNDLLNLKGDFYSTMNESCLFTFGKS"
     gene            1050..1241
                     /locus_tag="POH73_002564"
     CDS             1050..1241
                     /locus_tag="POH73_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888473.1"
                     /translation="MSVTNLRIAKEADLVTKNVLCNNSRKMHYIRSSYYSNVKVAYLR
                     GFLFYKSIKFYYNISCREQ"
     gene            1498..2673
                     /gene="rpsA"
                     /locus_tag="POH73_002565"
     CDS             1498..2673
                     /gene="rpsA"
                     /locus_tag="POH73_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDI6888474.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDDIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLESEEEDNPTIGDMIGDKLKNLKL"
     gene            2895..4205
                     /gene="der"
                     /locus_tag="POH73_002566"
     CDS             2895..4205
                     /gene="der"
                     /locus_tag="POH73_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDI6888475.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTDAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            4222..>4422
                     /locus_tag="POH73_002567"
     CDS             4222..>4422
                     /locus_tag="POH73_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDI6888476.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQEAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTK"
BASE COUNT         1638 a          578 c          864 g         1342 t
ORIGIN      
        1 tgtgacatac taatggtgcc accagtatga ataacaagta gatgtttcat atatttcctc
       61 ctatatttca tttacctaat tatgataaaa tattattcat aaaacgacaa ggaagggaaa
      121 tgacgcatga aagccattaa tattgcatta gatggtccag ctgctgccgg aaaaagtaca
      181 attgcaaaac gtgtagccag tgaactatca atgatttatg ttgatacagg agcaatgtat
      241 cgtgcattaa catacaaata tttaaaatta aacaaaactg aggactttgc aaaactagtt
      301 gaccaaacaa cattagattt aacttataaa gcagataaag gtcaatgtgt cattttagat
      361 aacgaagatg taacagactt tttaagaaat aatgatgtga cgcaacatgt ttcatacgtt
      421 gcatctaaag agccagtacg ttcattcgcc gttaaaaaac aaaaagagtt agctgcagaa
      481 aaaggtatcg taatggatgg tcgcgatatc ggaactgtag tgctaccaga tgcagattta
      541 aaagtatata tgattgcatc agttgaagag cgagcagaaa gaagatataa agataatcaa
      601 ttaagaggaa tcgaatcaaa ttttgaagat ttaaaacgtg atattgaagc acgtgatcaa
      661 tatgacatga accgtgaaat atcaccatta agaaaagcag atgatgcagt gacattagat
      721 acgacaggca agacgattga agaagttact gacgaaattt tagcgatggt gagtcaaatt
      781 aaataatcta gtaagtcata aatatctatt tgtaatgtgc aacgcttaat gcatatacaa
      841 attgaatgtg ctgataatga tttactcaac ttaaaaggtg atttttattc aacgatgaat
      901 gaaagttgcc tttttacttt tggtaaaagt taatgcgtca gtcaagtgtg tatgtttttc
      961 aaaaagttaa cagaaataat aaaggtaaat tattagaatt ccaaaaataa ttcattaaat
     1021 tcataaagca ttttacaaat ggtaagaaaa tgagtgttac aaatctaaga attgcaaaag
     1081 aagctgattt agtcacaaaa aatgtcctat gtaataattc gagaaagatg cactatatac
     1141 ggtcttctta ctattcaaat gtaaaagttg cttatttgcg tggctttttg ttttataaaa
     1201 gtataaaatt ttactataat atatcttgta gagaacaatg aaatgacaga cttataaaac
     1261 aaaatagtga gctatgctaa tttttataca aaaaatatgt ataaacatag cggactttat
     1321 tacttgtttg aatatatggt tgtaataaat atatgtccaa ctaatacaca gaatgagatt
     1381 aagtgacata tttatggata cgactaattt actaaatgta ataaaggtag aatttcttga
     1441 caattctgtc agtttataag atgttataaa tatgtagtgt ataaggaggc aaacaagatg
     1501 actgaagaat tcaatgaatc aatgattaac gatattaaag aaggtgacaa agtcactggc
     1561 gaggtacaac aagttgaaga caagcaagtt gttgttcata tcaacggtgg taaatttaat
     1621 gggattattc ctattagtca actatctacg catcatattg atagcccaag tgaagttgta
     1681 aaagagggcg acgaagttga agcatatgtc actaaagttg agtttgatga agaaaatgaa
     1741 actggagctt acatcttatc tagaagacaa cttgaaactg agaagtctta tagttattta
     1801 caagaaaaat tagataataa tgaaatcatc gaagcgaaag taacagaagt agttaaaggt
     1861 ggtttggttg ttgatgtagg acaaagaggt tttgttccgg cttcactaat ttcaacagac
     1921 ttcattgagg atttctctgt gtttgatgga caaacaattc gtattaaagt tgaagaattg
     1981 gatcctgaaa ataatagagt cattttaagc cgtaaagcag ttgaacaaga agaaaacgat
     2041 gctaaaaaag atcaattatt acaatcttta aatgaaggcg atgttattga tggtaaagta
     2101 gcgcgtttaa ctcaatttgg tgcatttata gacattggcg gtgtcgatgg tttagtgcat
     2161 gtatctgaac tttctcacga acatgttcaa acaccagaag aagtagtttc aattggtcaa
     2221 gatgttaaag ttaaaattaa atctattgat agagatacag aacgtatttc attatcaatc
     2281 aaagatacgt taccaacacc tttcgaaaat attaaaggtc aattccacga aaatgatgac
     2341 attgaaggtg tcgtagtaag attggcaaac tttggtgcat ttgttgaaat tgcaccaggt
     2401 gtacaagggc ttgtacatat ttctgaaatt gcacacaaac acattggtac gccaggtgaa
     2461 gtattagaac ctggtcaaca agtaaatgtt aaaatattag gtattgatga agagaatgaa
     2521 agagtatcac tatctattaa agcaacatta ccaaacgaag atgttgtaga aagtgatcct
     2581 tctacgacta aggcgtactt agaaagcgaa gaagaagata atccaacaat tggcgatatg
     2641 attggtgata aacttaaaaa tcttaaacta taatttaata tttaatagtc aactccacat
     2701 gtttatgatt gcatgtggag tatttttatg taacaaaata tatccggaat gataacgtgg
     2761 acaaatttaa ctaagtgttt tttaagataa agttttaagt gctgattttt atcattacag
     2821 taataaactc attttgaata cacagtctca tgtgatatta ttaaaaagat ataagaaaga
     2881 gaggaagtta gcttatgact aaacctatag tagctattgt aggtaggcct aatgtaggta
     2941 aatctacaat ttttaataga atagttggag aacgtgtttc gattgttgaa gatacgccag
     3001 gtgtaacacg agatcgtatt tattcttcag gtgagtggtt aacacatgat ttcaatatta
     3061 ttgatacagg tggtattgaa attggtgatg caccattcca aacacaaatt agagcgcagg
     3121 cagaaatcgc catagatgaa gcggatgtta ttatttttat ggttaacgtg cgtgaaggat
     3181 tgacacaaag cgatgaaatg gtcgctcaaa ttttatacaa atctaaaaaa ccggtcgtat
     3241 tagcggttaa caaagtagat aatatggaaa tgcgtacaga cgtgtatgat ttctattcat
     3301 taggatttgg tgaaccgtat ccgatatcag ggtcacatgg tttaggtctt ggtgacttgt
     3361 tagatgcagt tgtttctcat tttggtgaag aggaagaaga tccttatgat gaagatacaa
     3421 ttcgactatc cattattgga cgaccaaacg taggtaaatc aagtttagta aatgctattt
     3481 taggtgaaga tcgcgttatc gtttctaatg ttgcagggac aacgagagac gctattgata
     3541 cagagtatag ttatgatgga caagattatg ttttaatcga tactgctggt atgcgtaaaa
     3601 aaggaaaagt atatgaatca actgagaaat attcagtatt aagagcttta aaagcgattg
     3661 aacgttcaaa tgttgtttta gtggtcattg atgcagaaca aggcattatt gaacaagata
     3721 aacgtgttgc aggatatgca catgaacaag gtaaagcagt cgtgattgtc gtaaataaat
     3781 gggatactgt ggaaaaagat agtaaaacga tgaagaaatt tgaagatgaa gtacgtaaag
     3841 aattccaatt tttagattat gcacaaattg cttttgtgtc tgctaaagaa cgcacaagat
     3901 tacgtacatt attcccttac attaatgaag caagtgaaaa ccataaaaaa cgtgttcaaa
     3961 gttcaacttt aaatgaagtc gttactgatg caatttccat gaaccctaca ccaacagaca
     4021 aaggtagacg tttgaatgtc ttttatgcaa cacaagttgc tatagaacca ccgacatttg
     4081 ttgtatttgt taatgatgta gaattaatgc atttttctta taaacgctat ttagagaatc
     4141 aaatccgtgc cgcttttggt tttgaaggta caccaattca tattatagct cgaaagagaa
     4201 attaacgatt gggggataac aatgactaaa attaccgttt ttggtatggg aagttttggg
     4261 acagcccttg ccaatgttct tgcagaaaat ggacatgatg ttttgatgtg gggtaaaaat
     4321 caagaagctg ttgatgaatt aaatacatgt catacaaata aaaagtattt aaaatacgcg
     4381 aaattagatg ttaacatcat cgctacttca gatatgacca aa
//
LOCUS       DAMZPP010000180         4379 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMZPP010000180 DAMZPP010000000
VERSION     DAMZPP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.181"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(167..352)
                     /locus_tag="POH73_002568"
     CDS             complement(167..352)
                     /locus_tag="POH73_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888477.1"
                     /translation="MSEYKKKIIELIESNLTGYEISKKTGVSQYVLSQLRQGKREVDN
                     LTLNTTEKLYEYANKVL"
     gene            complement(745..855)
                     /locus_tag="POH73_002569"
     CDS             complement(745..855)
                     /locus_tag="POH73_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888478.1"
                     /translation="MKITIKVRKTNKKEKIEFLIGTFIIILVILGFKIMK"
     gene            complement(1557..1763)
                     /locus_tag="POH73_002570"
     CDS             complement(1557..1763)
                     /locus_tag="POH73_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888479.1"
                     /translation="MNEIETIISEIEKLLTNNTPYSISKNSGVPRQTVTDLKVGNTKI
                     KDAKFKTIIKLYEYQKSSENNSEC"
     gene            complement(2060..2272)
                     /locus_tag="POH73_002571"
     CDS             complement(2060..2272)
                     /locus_tag="POH73_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888480.1"
                     /translation="MNQFKEIYNTIEKLLNDKSISNYRINQDTGVSYGGISELRSGKR
                     KVNNLTLETAEKLYNYQKQLEIMIED"
     gene            2958..3242
                     /locus_tag="POH73_002572"
     CDS             2958..3242
                     /locus_tag="POH73_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888481.1"
                     /translation="MTEFTPFFDGSNQLNYRKFVILRFHGVSKRKICKMYNFAYFRIL
                     EVCEMAKKNDYRFTYKDYKFLKQYGVSNTFICKMYHISIEDLEFFEVMNR"
     gene            3393..3713
                     /locus_tag="POH73_002573"
     CDS             3393..3713
                     /locus_tag="POH73_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HDI6888482.1"
                     /translation="MKPVLDIKKTLGQLNFLGVDEKYKYENGERTDKTVYAYKLASQE
                     QGEQITVKTPNKVELDYLQECELVEPDVKMYVQMSGDFGTIAYSWNAEDIKVVGSNSA
                     LKQK"
     gene            3727..4029
                     /locus_tag="POH73_002574"
     CDS             3727..4029
                     /locus_tag="POH73_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888483.1"
                     /translation="MDYYTADRLYRYTNSSNLSEPILNYVASRINWGDKVSLMTLAKE
                     IQSKFNDSYVKENTVKGRPKIYADLCLLCMSLSEAGHGRMLQVNLEDCIYIGDIDV"
     gene            4271..>4379
                     /locus_tag="POH73_002575"
     CDS             4271..>4379
                     /locus_tag="POH73_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDI6888484.1"
                     /translation="MEAVVDWVQVTFHITPISAVIEDVIGLPITLFKKRN"
BASE COUNT         1504 a          596 c          568 g         1711 t
ORIGIN      
        1 tgctaatttc gtgcaataaa ttctaaatga aacgacatac tcctttttca caaattggaa
       61 tctactattt ttgtatttta attattataa tatccattca ccatatgatt tttattaata
      121 gttaaatcaa ataaacatag cttgattaat ttatacagta gttaaattac aaaactttat
      181 tggcatattc atataatttt tctgttgtat tcagggttag attatctact tcgcgtttgc
      241 cctgtcttaa ttgtgaaagt acatattgag aaactccagt ttttttagaa atttcatatc
      301 ctgttaaatt actttcaatt aattcaatta tctttttctt gtattctgac atttttttca
      361 tctccatatc tgtacaatgt ttactacttt ttttcgaaat tctgtgaact caacgtattt
      421 tttatttata aatgatttta ttatttttct attcattaat ggctatcatc acttcataat
      481 agttaaattt catattttca atttttgttt ctacatatta acgacttttc atcatcaatt
      541 actttatcaa agacaaacga tttgtcttgt ataccttacg tcttttacta aacttcaatg
      601 tttaaccttt tatgtatttt tattagatta gtgtctttca atattcaaac tactaactcc
      661 agtagtctat taaatttgag atatagagat atctttagac tttaattttt ccggagtcat
      721 atagacttct caaatactat acatttattt cataattttg aaccctaata ttactaaaat
      781 aattataaac gtccctatta aaaattctat tttctctttt ttatttgttt ttcgcacttt
      841 aatagttatc ttcattttac taatggtgag aaagtaatat tttattcaaa gaatatgact
      901 tcgtattaaa tcaaatctca cattcttatt agtttgcaaa ttcaatagtc ctatcagaat
      961 taaaatcaaa acattcaaac tattttatcg tttttacttc ctcgaccatt ttcacctcct
     1021 tataatctat ataaattatg tttaccacac gattctttac acttgatgaa ttaattttgt
     1081 taagaaacat ttaggcatgt aactttgtaa taaaaacatt ttcaaataaa tgtaccaaaa
     1141 actttcatga aataaagtat acttttcaaa tctattaatt ttaaaaatca taaaatcaaa
     1201 tgctatatta gaatataatt atcacaaatc atcatatatg ccaattacac aatctaatat
     1261 aaaaaattat caatgaaaaa ttatgcgcat aaacattatt acaaaatatg tatagtatag
     1321 aatactgcat taaagttaac ttatttactg tgttcagcct tctatatcat cacttaaaga
     1381 tcatttaaat ttttatggaa atgataatat aataatgcat acttttgatt gtttaaacat
     1441 gtacaatttt aaaaacctag agataatttt attgaggtga acaaagtgtg gacacacaat
     1501 catgaaatta atagaaattt aatctcttgt ccatctaatc aaacaaggta tatttattag
     1561 cactctgaat tattttctga cgacttttga tactcatata acttgattat tgttttaaat
     1621 ttggcatcct ttattttagt attacctacc tttaaatcag taactgtttg acgtggtaca
     1681 cctgagtttt ttgaaatact atatggtgta ttgttagtta ataacttttc tatttcactt
     1741 ataatagttt caatctcatt catttttaat cacgctctct ttctatttat aattataatt
     1801 atcctaatgc agtaactacg tttagcaata cattgtttct ttgtagttgc caaactagtt
     1861 aattcataaa ctaatgccta atagtattgg cgttttttat tatatattga taaaatttct
     1921 tgtctcaatc tattctatat tatattattt gataattttt ttatttatat tcacattaag
     1981 ctatccatat ttaatatact ttaaaattag atgatttaaa aggctcactc catgatattt
     2041 caaaagatat ggccttattt taatcttcaa tcattatttc taattgtttt tggtaattat
     2101 agagtttttc cgctgtttct aaagttaaat tattcacttt tcttttcccg cttcttaatt
     2161 cacttatacc accataagaa acaccagtgt cttgattaat tctataatta gatattgatt
     2221 tatcatttag taatttttct attgtattat aaatttcttt aaactggttc ataatttttg
     2281 taaaaatgaa agaatagttt atttttttct agttaaatta ataataaata atattaaagt
     2341 tgcaatgtta agatagagag ttaatttttt ccttaattct cttgtcataa ataccacctc
     2401 cctatttaat gtggtaaagg tcttttgttc tttacctttt taaaagaacg atttgataaa
     2461 attaagcact tgaattatta tgctcgtcac agctattcga aatttaattt tgctattcgt
     2521 tctttttctt taaccattct tatcattttt tctcctttcc tctttatagt tatttaacat
     2581 tccacaaatt gttgtaagtg atatgtttga attcccatat ccaatgacaa gatttataat
     2641 ttgcaattct aaattataat aacctatatt tttcaaaatc actttgtttt atgattaaca
     2701 tccatctaag agcacctaaa ggtttttatg taatgtaata aacatttcca tttatacata
     2761 tacatcttgt aaaaaagaaa gaactccata tatactcaaa aggtatactg aagttctata
     2821 tattatataa aattattata ctattttgaa aaacataagt aaatcactaa acatgatttc
     2881 tttcactttt agaaaacttt taataggtgg tgtaaactct agttaacagt taattcttat
     2941 gaaaggaatt gataattatg actgagttta cacctttttt tgatggttca aatcaattaa
     3001 attatcgtaa atttgtaatt ttaagatttc atggtgtttc taaaagaaaa atttgtaaaa
     3061 tgtataattt tgcatatttt cgtatattag aagtttgtga aatggcaaaa aagaatgatt
     3121 atcgatttac ttataaggat tataaatttt taaaacaata tggtgtgtct aatacattta
     3181 tttgtaagat gtatcatatt tcaattgaag acttagaatt ttttgaggtg atgaaccgat
     3241 aggtttgatt aaaaatatga taggtcagga gaagcataga cactccatta tcaaaaatgc
     3301 agtggtacgg atttgcggga gttattccta ttcgtgcgac cctttagaag tcgtttaatt
     3361 ttattaaaaa ttttgtaaag gatggatata ttatgaaacc agtattggat attaaaaaaa
     3421 ctttaggaca attaaacttt ttaggtgtag atgaaaaata taaatatgaa aatggagagc
     3481 gtactgataa aactgtttat gcatataaat tagcgagtca agagcaaggc gaacaaatca
     3541 ctgttaaaac gcctaataaa gtagaattag actatttaca agaatgcgaa ttagttgaac
     3601 cagacgttaa aatgtatgtt caaatgtcag gcgattttgg aactatcgca tatagctgga
     3661 atgcagaaga tattaaagta gttggttcta atagtgcact taaacaaaaa taattaggtg
     3721 atgtaaatgg attattatac agcagataga ttatatcgct acacaaatag ttcaaatttg
     3781 agtgaaccta ttttaaacta tgttgctagt cgtataaatt ggggcgataa agtttcatta
     3841 atgactttag ctaaagaaat acagtctaaa tttaatgata gttacgttaa agaaaatacg
     3901 gtaaaaggtc ggcctaaaat ttacgctgac ctttgtctat tgtgtatgag cttgagtgaa
     3961 gcaggacatg gcagaatgct acaagttaat ttggaagatt gtatttatat tggcgatatt
     4021 gatgtttaac atatagtatt agtgatttaa catgaccgtg tagcttttag cgtatgcagg
     4081 cgcagaaatg caagcacgga gtgcgttaag catttgggcg gagcctgcga caaatacgcg
     4141 ttaaaaaagc gagaacggtc atgtatcaaa tcgcttgcga tttgatagga ggcataaata
     4201 aatatgactt taaaaaacca gtgtaccccc cttactaata gggggggtag agcgtacaaa
     4261 taaaagtgcc gtggaggcag ttgttgactg ggttcaggtc actttccata taacccctat
     4321 ttcagcagta attgaggacg ttatcggttt gcctataacg ttgttcaaaa aacgaaata
//
LOCUS       DAMZPP010000181         4373 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMZPP010000181 DAMZPP010000000
VERSION     DAMZPP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.182"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>4373
                     /gene="ebh"
                     /locus_tag="POH73_002576"
     CDS             <1..>4373
                     /gene="ebh"
                     /locus_tag="POH73_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HDI6888485.1"
                     /translation="PDATITWVSGQAPNKNNTTIGQDINVTAHILIDGETTPITKTAT
                     YKVVRTVPKQVFETARGVLYPGVSDMYDAKQYVKPVNNSWSTNAQHMNFQFTNSYGPS
                     KDVVGISTRDIRVTYDNHQTQIIKILSKVKPDPPRIDGNSVTYKAGLTNQQIKINNVL
                     SSSSIKLFKADNTPLTITNTTYGSGNTAVVTVSDALPNGEIKARSSISMNNVTYTTQD
                     EHGRAIDVTRNESVDSNDSASVHVTPQLQATTEGAVFIKGGDGFDFGHVERFIQNPPH
                     GATVAWHDNPDTWKNTVGNTHKTAVVTLPNGQGTRNVEVPVKVYPVANAKAPSRDVKG
                     QNLTNGTDAINYITFDPNTNTNGITAVWANRQQPNNQQAGVQHLNVDVTYPGISAAKR
                     VPVTVNVYQFEFPQTSYTTTVGGTLASGTQASGYAHIQNATGLPTDGFTYKWNNAATG
                     TNDANWAAMNKPNTAQVINAKYDVIYNGHTFATSLPAKFVVKDVQPAKPTVTETAAGV
                     ITIAPGANQTVNTHAGNVTTYADKLVIKRNGNVVTTFTRHNNTSPWVKEASAANVAGI
                     AGTNNGITVAAGTFNPADTIQAVATQGSGETISDEQRSDDFTVVAPQPNQATTKIWQN
                     GHVDITPNNPSGHLINPTQAMDIAYTEKVGNGAEHSKTLNAVRGQNNQWTIANKPDYV
                     TLDAHTGKVTFNANTIKPNSAITITPKAGTGHSASSNPSTLTAPAAHTVNTTEIVKDY
                     GSNVTAAEINNAVQVANKRTATIKNGTAMPTNLAGGSTTTIPVTVTYNDSSTEEVQES
                     IFTKADKRELITAKNHLDDPVSTDGKKPGTITQYNNAIHNAQQQINTAKTEAQQVIND
                     ERATPQQVNAALSKVQAAQTKINEAKALLQNKEDNSQLVTSKNNLQSSVNQVPSTTGM
                     TQQSIDNYNAKKREAESEITAAQRVIDNGDATAQQISDEKHRVDNALTALNQAKQNLT
                     ADTHELEQAVHQLNRTGTTTGKKPASITAYNNSIRALQSDLTSAKNSANAIIQKPIRS
                     VQEVQTALTNVNRVNDRLTLAINQLVPLADNSALRTAKTKLDEEINKSVTTDGMTQSS
                     IQAYENAKRAGQTESTNAQNVINNGDATDQQIAAKKTKVEEKYNGLKQAIAGLTPDLA
                     PLQAAKTQLQNDIDQPTSTTGMTSASVAAFNDKLSAARTKIQEIDRVLASHPDVATIR
                     QNVTAANATKTALDQARNGLTVDKAPLENAKNQLQQSIDTQTSTTGMTQDSINAYNAK
                     LTAARNKIQQINQVLAGSPTVDQINTNTSAANQAKSDLDHARQALTPDKAPLQTAKTQ
                     LEQSINQPTDTTGMTTASLNAYNQKLQAARQKLTEINQVLNGNPTVQNINDKVAEANQ
                     AKDQLNTARQGLTLDRQPAFTTLHGASNLNQAQQNNFTQQINAAPNHAALETIKSNIT
                     ALNNAMTKLKDSVADNNSIKS"
BASE COUNT         1695 a          841 c          794 g         1043 t
ORIGIN      
        1 tccagatgcg acgataacat gggtaagtgg tcaagcgcca aacaaaaata acacaactat
       61 aggtcaagat atcaatgtaa ctgcacatat cttaattgat ggtgaaacaa ccccaattac
      121 gaaaacagca acatataaag tggtaagaac tgtaccaaag caagtctttg aaacagccag
      181 aggtgtctta tacccaggtg tttcagatat gtatgatgcg aaacaatatg ttaagccagt
      241 aaataattct tggtcgacaa atgcgcaaca tatgaatttc caatttacaa attcatatgg
      301 accaagtaaa gatgtagtag gtatttcaac aagggatatc cgagttactt atgataatca
      361 ccaaacacaa attatcaaga ttttatctaa agttaagcct gatccaccga gaattgacgg
      421 aaactctgtg acatacaaag caggcttaac taaccaacaa atcaagatta ataatgtttt
      481 aagtagttct tctattaaat tatttaaagc agataataca ccattaacaa ttactaacac
      541 gacttatggt agtggaaata ctgcagtagt tactgtaagt gatgcgttac caaatggtga
      601 aattaaagca agatcttcga tttcaatgaa taatgtaact tatacgacac aagacgaaca
      661 tggacgagct atagatgtaa caagaaatga atctgttgat tcaaatgaca gtgcttctgt
      721 acatgtgaca cctcaattac aagcgactac tgaaggcgct gtatttatta aaggtggcga
      781 cggttttgat ttcggtcatg tagaacgatt tattcaaaat ccaccacatg gggcaacggt
      841 tgcatggcat gataatccag atacatggaa gaatacagtc ggaaacactc ataaaactgc
      901 ggttgtaaca ttaccaaatg gtcaagggac acgtaatgta gaagtacctg tcaaagttta
      961 tccagttgct aatgccaaag cgccatctcg tgatgttaaa ggtcaaaatt tgactaatgg
     1021 aacggatgcg ataaactaca ttacatttga tccaaataca aacacaaatg gtatcactgc
     1081 agtatgggca aatagacaac aaccaaataa ccaacaagca ggcgttcaac atttaaatgt
     1141 cgatgtaaca tatccaggta tttcagctgc aaaacgtgta cctgttactg tgaatgtata
     1201 tcaatttgaa ttccctcaaa cttcttatac aacaacagtt ggtggcactt tagcaagtgg
     1261 tacgcaagca tcaggatatg cacatataca aaatgctact ggtttaccaa cagatggatt
     1321 tacgtataaa tggaataatg cagcaacagg tacaaacgat gcaaactggg cagcaatgaa
     1381 taaaccaaat actgcacaag tcattaatgc aaaatatgat gtcatctata atggacatac
     1441 atttgcaaca tctctaccag cgaagtttgt agtaaaagat gttcaaccag cgaaaccaac
     1501 tgttacagaa acggcagcag gagtaattac gattgcacct ggagcaaacc aaacagtgaa
     1561 tacacatgcc ggtaacgtaa cgacatacgc tgataaatta gttattaaac gtaatggtaa
     1621 tgttgtgacg acatttactc gtcataataa tacgagtcca tgggtgaaag aagcatctgc
     1681 agcaaatgta gcaggtattg ctggaacgaa taatggtatt actgttgcag ctggtacttt
     1741 caatcctgct gatacaattc aagctgttgc aacacaaggt agtggtgaaa caatcagtga
     1801 cgaacaacgt agtgatgatt tcacagttgt cgcacctcaa ccgaaccaag cgactactaa
     1861 gatttggcaa aatggtcatg ttgatatcac gcctaataac ccgtctggac atttaattaa
     1921 tccaactcaa gcaatggata ttgcttacac tgaaaaagtg ggtaatggtg cagaacatag
     1981 taagacactt aatgctgttc gtggtcaaaa taatcaatgg acaatcgcca ataaacctga
     2041 ctatgtaacg ctagatgcac atactggtaa agtgacgttc aatgccaata ctattaagcc
     2101 aaattcagca atcaccatta caccgaaagc aggtactggt cactcagcaa gcagtaatcc
     2161 aagtacatta actgcaccgg cagcacatac tgtcaacaca actgaaattg taaaagatta
     2221 tggttcaaat gtaacagcag ctgaaattaa caatgcagtt caagttgcta ataaacgtac
     2281 tgcaacgatt aaaaatggca cagcaatgcc tactaattta gctggtggta gtacaacgac
     2341 gatccctgtg acagtaactt acaatgatag cagtactgaa gaagtacaag agtccatttt
     2401 cacaaaagcg gataaacgtg agttaatcac agctaaaaat catttagatg atccagtgag
     2461 tacagatggt aaaaagccag gtacaattac gcagtacaat aatgcaattc ataatgcgca
     2521 acaacaaatc aatactgcga aaacagaagc gcaacaagtg attaatgatg agcgtgcaac
     2581 accacaacaa gtgaacgcag cattatctaa agttcaagct gcacaaacta agataaatga
     2641 agctaaagca ttacttcaaa ataaagaaga taatagccaa ttagtaacat ctaaaaataa
     2701 cttacaaagt tctgtgaacc aagtaccatc aactactggt atgacgcaac aaagtattga
     2761 taactataat gcgaagaaac gtgaagcaga atctgaaatt acagcagctc aacgtgtcat
     2821 tgataatggt gacgcaacag cacaacaaat ttcagatgaa aaacatcgtg tcgataacgc
     2881 attaacagca ttaaaccaag cgaaacaaaa tttaacagca gatacacatg aattagagca
     2941 agcagtacat caattgaatc gtacaggtac aacgacaggt aagaaaccag caagtatcac
     3001 tgcatacaat aattcgattc gtgcacttca aagtgactta acaagtgcta aaaatagtgc
     3061 caacgctatt attcagaagc caattagatc agtgcaagag gtacaaacag cattaacgaa
     3121 tgtaaatcgt gtaaatgacc gattaacgct agctattaat caattagtac ctttagctga
     3181 taatagtgct ttaagaactg ctaaaacgaa acttgatgaa gaaatcaata aatcagtaac
     3241 tactgatggt atgacacaat catcaatcca agcatatgaa aatgctaaac gtgcgggtca
     3301 aacagaatca acaaatgcac aaaatgttat taacaatggt gacgcaacag accaacaaat
     3361 tgccgcaaaa aaaactaaag tagaagaaaa atataatggc ttaaaacaag ctattgccgg
     3421 attaacacca gacttggcac cattacaagc tgcgaaaact cagttgcaaa atgatattga
     3481 tcaaccaacg agcacgacag gtatgacaag tgcatcagtt gctgcattta atgacaaact
     3541 ttcagcagct agaactaaaa ttcaagaaat tgatcgcgta ctagcatcgc atccagatgt
     3601 tgcaacaatt cgacaaaacg tgacagcagc gaatgctact aaaacagcac ttgatcaagc
     3661 acgcaatggc ttaacagtag ataaggcgcc tttagaaaat gcgaaaaatc aactacaaca
     3721 aagtattgat acgcaaacaa gtacaactgg tatgacgcaa gattctatta atgcatacaa
     3781 tgcgaagtta acagctgcac gtaataagat tcaacaaatc aatcaagtat tagctggttc
     3841 acctactgta gatcaaatta atacaaatac gtctgcagca aatcaagcga aatctgattt
     3901 ggatcatgcg cgtcaagctt taacaccaga taaagcaccg cttcaaactg cgaaaacgca
     3961 attagaacaa agcattaatc aaccaacgga tacaacaggt atgacgaccg cttcgttaaa
     4021 tgcgtacaac caaaaattac aagcagcgcg tcaaaagtta actgaaatta atcaagtgtt
     4081 gaatggcaac ccaacggtcc aaaatatcaa tgataaagtg gcagaggcaa accaagctaa
     4141 ggatcaatta aatacagcac gtcaaggttt aacattagat agacaaccag cgtttacaac
     4201 attacatggt gcatctaact taaaccaagc acaacaaaat aatttcacgc aacaaattaa
     4261 tgctgcacca aatcatgctg cacttgaaac gatcaagtct aacattacag ctttaaataa
     4321 tgcgatgaca aaattaaaag acagtgttgc ggataataat tcgattaaat cag
//
LOCUS       DAMZPP010000182         4307 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMZPP010000182 DAMZPP010000000
VERSION     DAMZPP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.183"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..147
                     /locus_tag="POH73_002577"
     CDS             <1..147
                     /locus_tag="POH73_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6888486.1"
                     /translation="SLVNDGRAMLLIYPHELFIPAMILSLLILFFYLFSDGLRDAFDP
                     KMRK"
     gene            163..1245
                     /locus_tag="POH73_002578"
     CDS             163..1245
                     /locus_tag="POH73_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6888487.1"
                     /translation="MTERILELNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKLALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            1235..2176
                     /locus_tag="POH73_002579"
     CDS             1235..2176
                     /locus_tag="POH73_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6888488.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFEIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQREKGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPADDIYQYPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPQHFVFSTEEEAARLRKNLLVTQD"
     gene            2195..3850
                     /locus_tag="POH73_002580"
     CDS             2195..3850
                     /locus_tag="POH73_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6888489.1"
                     /translation="MTRKLKTLILIFVATIALSGCANDDGIYSDKGQVFRKILSADLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALD
                     DETLQIDLEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKKVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNELSFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            4062..>4307
                     /locus_tag="POH73_002581"
     CDS             4062..>4307
                     /locus_tag="POH73_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6888490.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTEASKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADAVVEGYFNENLLT"
BASE COUNT         1648 a          602 c          826 g         1231 t
ORIGIN      
        1 tcattagtaa atgatgggcg cgcaatgtta ttaatttatc cacatgaatt atttatacca
       61 gcaatgattt taagtttatt aattctattc ttttacttat ttagtgatgg attacgtgat
      121 gcatttgatc cgaaaatgcg taaataaaag ggggcatagc atatgactga aagaatatta
      181 gaactaaatg atttgcatgt ttcctttgat attacagcag gggaagtgca ggcagtgaga
      241 ggtgtagatt tttatttaaa caaaggggaa acattggcaa ttgttggtga atcaggttca
      301 ggtaaatctg tgacaacaaa agcaattaca aaattattcc aaggggacac aggaagaatt
      361 aaaaagggag aaattttatt tttaggggaa gatttagcaa aaaaacctga aaatgagtta
      421 attaaattac gtggaaaaga tatttcaatg atctttcaag atccaatgac atctttaaac
      481 ccaacgatgc aaattggtaa acaagtcatg gaaccattaa ttaagcacaa aaattatagt
      541 aaagcacaag ctaaaaagct cgcattggaa atactaaatc ttgtaggatt accaaatgca
      601 gaaaaaagat ttaaagcata tccacatcaa ttttcaggtg gacaaaggca aagaattgtt
      661 attgcaaccg cattagcttg tgaacctaaa gtgctgattg ctgatgaacc aacgactgca
      721 ttagacgtaa cgatgcaggc acaaatttta gatttaatga aagaactaca acaaaaaatc
      781 gatacagcaa ttatttttat aacgcatgat ttaggggttg ttgcgaatat tgctgataga
      841 gtggcagtta tgtatggtgg tcaaatggtt gaaacaggag atgttaacga aatattttat
      901 gatccaaagc atccatatac atggggatta ttatcgtcaa tgcctgatct atcaacaaca
      961 aatgacacac cattactagc gattcctgga gcgccacctg atttattaca cccacctaaa
     1021 ggtgatgcat ttgcgagacg tagtcaatat gcattagata ttgattttaa agtagaaccg
     1081 ccgtggttta aagtttcacc gacacatttt gtgaaatctt ggttattaga cgcacgtgca
     1141 ccaaaagttg aactacccga gctggtaaaa caacgtatga aaccgatgcc taataattat
     1201 gaaaaaccac tcaaggtaga aagggtgtcg ttcaatgaaa aatgatgaag tgctattatc
     1261 tattaaaaat ttaaagcaat attttaacgc aggaaagaaa aacgaagtga gagcgattga
     1321 aaatatttcg tttgaaatat acaaaggaga aacattaggt ttagtaggag aatcggggtg
     1381 tggtaaatct acaactggta aatcaattat caaacttaat gatattacaa gtggagaaat
     1441 tttgtatgag ggtattgata tacaaaagat tcgtaaacgt aaagatttgc ttaaatttaa
     1501 taaaaagata cagatgattt ttcaagaccc atatgcgtct ttaaatccta ggttaaaagt
     1561 aatggatata gtagctgaag gtattgatat ccatcattta gcaactgata agcgtgaccg
     1621 taaaaaacgt gtctatgatt tacttgaaac tgttggatta agtaaagaac atgccaatcg
     1681 ttatcctcat gaattttcag gtggacaacg ccaacgtatt ggaattgccc gtgcattagc
     1741 cgttgaacca gaatttatta tagcagatga acctatttca gcattggatg tttcaattca
     1801 agctcaagta gtcaatttgt tattaaaatt acagcgtgaa aaaggtatta cattcctatt
     1861 tattgcacat gatttatcga tggtgaagta tatctctgat cgaattgcag tcatgcattt
     1921 tgggaaaatt gtagaaatcg gaccagcaga cgatatttat caatatccat tacatgatta
     1981 tactaagtct ttattgtcgg caattcctca accggatcct gaatcggaac gtagtcgtaa
     2041 acgatttagt tatattgatg atgaagcaaa taatcattta agacaattac atgaaataag
     2101 accacagcat tttgtcttta gtactgaaga agaagcggca cgactacgaa aaaatttgtt
     2161 ggtaacacaa gattaagggg aagggagaaa tgaaatgaca agaaagttaa aaacgctgat
     2221 tttaatattt gttgccacaa ttgcattaag tggttgtgct aatgatgatg gtatttattc
     2281 agataaaggt caagtattca gaaaaatttt gtcagcagac ttaacatcac ttgatacatc
     2341 attaattacg gatgaaatat cttctgaagt gactgcgcaa acattcgaag gtttatatac
     2401 attaggaaaa ggtgacaaac cggtgttagg tgttgcgaaa gcttttcctg aaaagagtaa
     2461 agatgggaaa actttaaagg ttaaattaag aagcgatgct aaatggagca atggtgacaa
     2521 agtgactgca caagactttg tttatgcttg gagaaaaaca gttgacccta aaactggttc
     2581 tgaatttgca tacattatgg gggacattaa aaatgcgagt gatattagta ctggtaagaa
     2641 acctgtagag caattaggta tcaaagcatt agatgatgaa acattacaaa ttgatttaga
     2701 aaagccggta ccatacatta atcaattatt agcacttaat acatttgcac ctcaaaatga
     2761 aaaagttgcc aaaaaatatg gtaaaaatta tggtacggca gctgatagag cggtatacaa
     2821 tggtccattt aaagttgatg attggaaaca agaagataaa acgttactat ctaaaaatca
     2881 gtattattgg gataaaaaga aagtaaaatt agataaagtg aattataaag ttattaaaga
     2941 cttacaagca ggtgcatcat tgtatgatac tgaatctgta gatgacgcag ttattacagc
     3001 agatcaagta aataaatata aagataataa aggattaaac tttgtgttaa cgactgggac
     3061 attttttgta aaaatgaatg aaaaacaata tcctgatttt aaaaacaaaa atttaagact
     3121 ggctatcgca caagcaatag ataaaaaagg atacgttgat tcagtgaaaa acaatggttc
     3181 aattccttcc gatacactaa cagccaaagg cattgcgaaa gcacctaatg gcaaagatta
     3241 tgcgagtacc atgaattcac ctctaaaata taatcctaaa gaagcaagag cgcactggga
     3301 caaagctaaa aaagagttag gtaaaaatga attgtcattt tcgatgaaca cagaagatac
     3361 accagatgca aaaatatctg ctgaatacat caaatcacaa gttgagaaaa atttaccagg
     3421 agttactttg aaaattaagc aattgccgtt taaacaaaga gtatcactag aactgagtaa
     3481 caattttgaa gcatcactta gtggttggtc tgcagattac cctgatccta tggcttattt
     3541 agaaacaatg accacaggta gcgcacaaaa taatacagac tggggtaata aagaatacga
     3601 tcaattactt aaagtagcga gaaccaaatt ggcacttcaa ccgaacgaac gatatgaaaa
     3661 cttgaaaaaa gcagaagaaa tgttcctagg agatgcaccg gtagcaccaa tttatcaaaa
     3721 aggtgttgca catttaacaa atcctcaagt aaaaggattg atttaccata aatttggtcc
     3781 aaataactca cttaaacatg tatatattga taaatcgata gataaagaaa caggtaagaa
     3841 gaaaaaataa tttgctttgt aaattagact ggagatatat ctccagtctt tttgtgttgc
     3901 agaaaaactt tgggaataaa aaattaaaat aagtcgtttt ttaaattact gaaattgatt
     3961 aaatgcataa atgactgaat attctaaaaa taaacttgta ataatttttt ctatgagtaa
     4021 actaaaaaga aaatattaga ttgaaagtag gaggcatatg tatggggaag cttattaaat
     4081 atatttcaat acttcttatt gtcgttttag tgttgagcgc ttgcggaaaa agcagtaata
     4141 aagatgaagg agtaaaagat gctactaaaa cagaagcgtc aaaacataaa ggtggtactc
     4201 taaatgtagc gttaacagca ccgccaagtg gtgtttattc ttcgttatta aatagtacac
     4261 atgcggatgc tgtagttgaa ggatatttta atgaaaactt attaaca
//
LOCUS       DAMZPP010000183         4274 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMZPP010000183 DAMZPP010000000
VERSION     DAMZPP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.184"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(48..311)
                     /locus_tag="POH73_002582"
     CDS             complement(48..311)
                     /locus_tag="POH73_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6888491.1"
                     /translation="MPKIIIPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYREDNLGVENLYIDYSATGTLINISKLEEYLIRKHKKWY"
     gene            complement(336..551)
                     /locus_tag="POH73_002583"
     CDS             complement(336..551)
                     /locus_tag="POH73_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888492.1"
                     /translation="MNTRSEGLRIGVPQVSSKADASSSYLTEKERNLGAEILELIKKS
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            606..971
                     /locus_tag="POH73_002584"
     CDS             606..971
                     /locus_tag="POH73_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888493.1"
                     /translation="MPLISDEFDTLTKDQQYILSVLYKDYLECVKLGSVKLTCNNFGS
                     AKDIHTKYFQKLHFEDVKYDLNKLKNSGFLNGVYASNTIYHVTISDKTVVYFENEFKN
                     NLKSIIDSISKIASIIPGL"
     gene            complement(940..1185)
                     /locus_tag="POH73_002585"
     CDS             complement(940..1185)
                     /locus_tag="POH73_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDI6888494.1"
                     /translation="MNIQEATKIATKNLVSMTRKDWKESHRTKILPTNDSFLQCIISN
                     SDGTNLIRYWQPSADDLMANDWEVINPTRDQELLKQF"
     gene            complement(1235..1453)
                     /locus_tag="POH73_002586"
     CDS             complement(1235..1453)
                     /locus_tag="POH73_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888495.1"
                     /translation="MNEEKVKVVQQVLGQVEQEISFALGTDEGSQIKKKIEHNGREYE
                     IYMTREECLEDTLIEITKLLEAINFGFY"
     gene            complement(1469..2245)
                     /locus_tag="POH73_002587"
     CDS             complement(1469..2245)
                     /locus_tag="POH73_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDI6888496.1"
                     /translation="MQALQTFNFEELPVRTLTVDNEPYFVGKDVAEILGYSNTRDALS
                     KHVDEDDKEILTSRNTTLENLPNRGLTAVNESGLYSLIFSSKLESAKRFKRWVTSDVL
                     PAIRKHGIYATDSVIENTLNNPDYIINILTEYKKEKEQNLLLQQEMGELKPKADYVDE
                     ILKSTGTLATTQIAADYGISAQKLNKLLHEARLQRKVNKQWVLYSEHMGKSYTESDTI
                     PIVRSDGREDTVLQTRWTQKGRLKIHEIMTEFGYEANVTA"
     gene            complement(2271..2498)
                     /locus_tag="POH73_002588"
     CDS             complement(2271..2498)
                     /locus_tag="POH73_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BetR domain protein"
                     /protein_id="HDI6888497.1"
                     /translation="MNKAKLYSALAMKEMHVNDFLKELNNHGLKLSKSAYYSRIRGEQ
                     EFDIKEIKTIVKVLNLTREEMNDIFFGELVS"
     gene            2670..3284
                     /locus_tag="POH73_002589"
     CDS             2670..3284
                     /locus_tag="POH73_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6888498.1"
                     /translation="MKPDIKSRRKELNLTLEQVGDLVGVGKSTVRKWETGDIENMKRD
                     KIVKLAKALRVSPSYIMGIEDEKPQLETIPVKKIPVVSKISAGMPIYTEENLIDYIYF
                     ATKNLNSNKEEFGLQVSGDSMDKLFQDGDVVVVEKDSTVENGQLGVVLVNGYNGTVKR
                     IRYNNDQIILIPESNNPSHYPQVYGKDDEVKIVGRVVASQKLFK"
     gene            3300..4220
                     /locus_tag="POH73_002590"
     CDS             3300..4220
                     /locus_tag="POH73_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDI6888499.1"
                     /translation="MDFVAIDVETANEQRSSICSIALLKFQDNKMRDAIYTHINPETY
                     FSSINTSIHGISEEDVMDSPKMYQMHEAIIRFLDNHIIVAHNTSFDMYALCDSFKKYN
                     LPLPSNQYFCTYRLSKDLYNLPSYRLQDLAEHFDIKNEEYHNAFYDAKVCAKIAYNIL
                     KDQDANLNDLVENQNFKYGVFGQNGFIKKSANKKIELVYNEELNDKNHILYGKHVCFT
                     GALKGFTRNEIAQTVTNIGSVYDSSVKKSTNYLVVGNLENLEKTHNYTKSSKIKKAEK
                     LSSEGQDIEILSEMDFLKLLTTFTSVETKT"
BASE COUNT         1341 a          707 c          684 g         1542 t
ORIGIN      
        1 tactgctaat aggtagcttt tatatgtgtc gctcatttaa tatcctccta ataccatttt
       61 ttatgctttc tgatcaaata ctcttctaat ttagaaatat taatcaatgt tcccgttgct
      121 gaataatcaa tgtataaatt ttctacacct aaattatctt cacggtaata tttcaaccag
      181 ttgtatactg tacttctaca tactccaaac aattgatgga tttgtgtagg tgttgcgtat
      241 aactttttca caaatttttc ttcgcctcga tatgtgtttt ctggtgttgg tggtattatg
      301 atttttggca tctctatcac tcctttagat aaatgttaaa gtttgttatt attcgccctg
      361 tattgaagtt ctctatctaa tgcatagaaa actttgttta tttctaagta gctgtaatca
      421 ctttttttaa taagctctaa tatttccgct cctaagttac gttccttttc cgttaaatag
      481 gatgaagaag catcagcttt gctagaaact tgtgggacgc ctatacgcaa tccttctgat
      541 cttgtgttca tttgtttatg ctcctttcgt gtataatgtt gttatcaacc taaggaggtg
      601 ataacatgcc cttgatatct gatgaatttg atacacttac taaagaccaa caatatatct
      661 tgtccgtact ctacaaagat tatttagaat gtgtaaagtt aggttcggtt aaattaacct
      721 gcaataattt tggaagtgct aaagatatac atacaaagta ttttcaaaaa ctacatttcg
      781 aagatgtaaa atacgattta aataaactta aaaactctgg gttcctaaac ggcgtgtatg
      841 ctagtaacac tatttatcat gtaacaattt cagacaagac tgttgtttac tttgaaaatg
      901 agtttaaaaa caatttaaaa agtatcattg atagcatttc taaaattgct tcaataattc
      961 ctggtctcta gttgggttta taacttccca atcatttgcc atgaggtcat cggctgaagg
     1021 ttgccaatat ctgataaggt ttgtcccatc gctatttgaa atgatgcatt gtaaaaaact
     1081 atcatttgtt ggtaatatct tagttcgatg actttctttc caatctttcc gtgtcataga
     1141 gacaagattt tttgtagcta tcttagttgc ttcttgaatg ttcatttgtt attcctcctt
     1201 ttaagatgtt tgttcgattg gggtttaatt tgttttaata aaacccgaaa ttaattgctt
     1261 ctagtagttt agtaatttca atcaaggtat cttctaaaca ttcttctctt gtcatataga
     1321 tttcgtattc cctaccgtta tgttcaattt tctttttgat ttgtgatcct tcatctgtac
     1381 caagagcaaa gcttatttct tgttcaactt gtcctaaaac ttgttgtact acttttactt
     1441 tttcttcatt catttttaag tcctcctatt aagctgttac gttagcttca taaccaaatt
     1501 cagtcatgat ttcgtgtatt tttaatctgc ctttttgcgt ccatctagtt tgtagcactg
     1561 tgtcttctct accgtcagag cgtacaattg gtatagtgtc tgattctgtg taactcttgc
     1621 ccatgtgttc tgagtaaagc acccactgtt tatttacttt tcgttgtaat ctagcttcgt
     1681 gtagtagttt gtttaacttt tgtgctgaaa tcccatagtc tgctgcaatc tgagttgtag
     1741 ctaatgtgcc agttgatttt aagatttcat caacataatc tgctttaggc ttcaactctc
     1801 ccatttcttg ctgtaatagt aagttttgct ctttttcttt cttatactca gtaagaatgt
     1861 taatgatgta atctggattg ttcagcgtgt tctcaattac actgtctgtt gcgtagatac
     1921 cgtgtttgcg aatggctggt aggacgtctg atgttaccca gcgtttgaat cgttttgctg
     1981 actctagttt tgatgagaag attaagctgt ataaacctga ttcgttgact gcagtaagtc
     2041 ctcgatttgg caaattttct aaagtcgtgt ttcgcgacgt tagaatttcc ttatcgtctt
     2101 catcaacgtg tttacttaat gcgtcacgcg tattcgagta tcctaagatt tctgccacat
     2161 ctttacctac aaaatatggt tcgttatcta ctgttaatgt tcttactggt aattcttcaa
     2221 aattaaatgt ttgtaatgct tgcatttgtt tatgcctcct caagtgtctt ttaggacacc
     2281 aattctccaa aaaaaatatc gttcatttct tctctggtta aattaagaac tttgactatc
     2341 gtcttaattt ctttgatgtc aaattcttgt tctcctctaa ttctactata ataagcgctt
     2401 ttagaaagtt ttaaaccatg attattcaat tcttttaaaa aatcatttac atgcatctct
     2461 ttcatcgcta aagcagaata aagtttcgct ttgttcattt ttcaacacct ccgtgtcttt
     2521 taggatactt caatgataca tgctaatatc aatctagtca acacaaaagt gtctaaaaat
     2581 acatttttat ttttacttcc tatataagtg ttgcttttaa gaaactttag tgttatattc
     2641 agtacatacc tattaggagg aaatttaaaa tgaaaccaga cattaaaagc agacgtaaag
     2701 aattgaattt aactctagaa caagttggag atttagttgg tgtaggtaaa tccacagtaa
     2761 gaaaatggga aactggcgat atcgaaaata tgaagagaga taaaatagta aaattagcta
     2821 aagcattaag agtatctcca tcatatatca tgggtattga agatgagaaa cctcaacttg
     2881 aaaccatacc agttaaaaag ataccggttg tatcaaagat atctgcggga atgcccatat
     2941 atacagaaga gaatttaatc gactacatat actttgctac taaaaatttg aactctaata
     3001 aagaagagtt cggattgcaa gtgtctgggg atagtatgga taaactcttt caagacggag
     3061 acgttgtagt tgttgaaaag gattcgactg ttgagaatgg ccaattaggt gttgtattag
     3121 tcaatggata caacggtact gtcaaaagaa tacgttacaa caatgaccaa attattttaa
     3181 ttcctgaatc aaacaatcct agtcactacc cacaagtata tggaaaagat gacgaggtta
     3241 agattgtagg tagagttgta gcaagtcaaa aactattcaa ataaaagggg ctgattataa
     3301 tggattttgt agcgatcgat gtcgaaacag ctaacgaaca acgcagttct atttgttcaa
     3361 tagcactatt aaaatttcaa gacaacaaaa tgcgtgatgc tatttatact cacataaatc
     3421 ccgaaactta tttttcttct ataaacactt ctatacatgg aatatccgaa gaagacgtaa
     3481 tggattcccc taaaatgtat caaatgcatg aagctattat tagattctta gataaccata
     3541 taatagtagc ccataataca tctttcgata tgtatgctct atgtgattct ttcaaaaaat
     3601 acaatctacc tttacctagc aatcaatatt tttgtactta tcgattaagt aaagatttat
     3661 ataatttacc ttcatataga ctccaagatt tagctgaaca ttttgacatt aaaaacgaag
     3721 aatatcacaa tgctttttat gatgctaaag tgtgtgccaa aattgcatat aacatattaa
     3781 aagaccaaga tgcaaacttg aacgatttag tcgaaaacca aaattttaag tacggtgtgt
     3841 ttggtcagaa tggattcata aaaaaatcgg ctaataagaa aatagagcta gtatacaatg
     3901 aagagttaaa cgataaaaat catatattat atggtaaaca tgtttgtttt accggagcgt
     3961 taaaaggttt cacacggaac gaaatagccc aaactgtaac taatattggt tctgtttacg
     4021 attcctctgt aaaaaagtca actaactacc tcgttgtcgg taatttagaa aacctagaaa
     4081 aaactcataa ctataccaag tcaagtaaga ttaaaaaagc agaaaaatta tcaagtgaag
     4141 gacaagacat tgaaatttta agtgaaatgg atttcttaaa attattaact accttcactt
     4201 ctgtagaaac gaaaacgtga tgtcggttga taatgtttta ttgtgattaa tagcgcctat
     4261 atggcacttt aata
//
LOCUS       DAMZPP010000184         4040 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMZPP010000184 DAMZPP010000000
VERSION     DAMZPP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.185"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..185)
                     /locus_tag="POH73_002591"
     CDS             complement(<1..185)
                     /locus_tag="POH73_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDI6888500.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPN"
     gene            complement(211..1788)
                     /locus_tag="POH73_002592"
     CDS             complement(211..1788)
                     /locus_tag="POH73_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDI6888501.1"
                     /translation="MEVTCLKQITWHDLQHIIKDDDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGVAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     IDDETYLHYTFPSVDVALLRGTYADQQGNIYLTQESYLSECYHVALNAKANHGKVIVQ
                     VKDLVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEVYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGELKQSIHKV"
     gene            2147..2818
                     /locus_tag="POH73_002593"
     CDS             2147..2818
                     /locus_tag="POH73_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDI6888502.1"
                     /translation="MRVNVLQHTPNEGVGYIGEWAKERGHEVYIYHPYMNDGVLPLAE
                     ETDMLVILGGPMNPDDEMPWIQSERQLILVLMAQNKPIYGACFGAQQISKALGYAVLK
                     SPVKEVGWDDVYLESEIIPNIPSRLKALHWHEDMFQIPQQAELLFSGNHIRNQGFIID
                     HHVIGLQFHFEPGPDDVREIVLNDAQYINHSILEQSAEDILAFEVPKQNRQAIFRILD
                     FITQA"
     gene            3042..>4040
                     /locus_tag="POH73_002594"
     CDS             3042..>4040
                     /locus_tag="POH73_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDI6888503.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMFSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVGLHGIWDTSLT"
BASE COUNT         1312 a          667 c          763 g         1298 t
ORIGIN      
        1 tcatttggcg caaaaatacc gataacatcg ccttttttaa caccttgaga tgttaaataa
       61 tgtgccatat tatcagcgcg tgcattgagt tgttggtatg tccaagatgt ttgttttgcg
      121 tgatcaataa cggcaggctt gtcatcatcg aagtctgagc gcgtttttat ccaatcgaaa
      181 ttcattagta tacccccttt agcttcactt tcatacttta tgaattgatt gtttaagttc
      241 tccccatttt tctttgtaaa tgctggtatc aattaatttt aaatgatcag caataattgg
      301 tttaaaagcc atttgattca aaatatcttt atgcaaatca agacctggtg caatttcaat
      361 tagtttcaag ccttgattgg tgagctcgaa tactgcacga tcagtaacaa aatagacttc
      421 ttgctcgagt gattgtgaat attgtgcatt aaagtcgata tggctcacat ctgatacaaa
      481 tttctggttt tgtccttcag tttcaatgtt taatcgttga ttatgacatg agacatgact
      541 gccagctaca aaagtacctg aaaagataat tttatttaca gattgtgtaa tgtctataaa
      601 gccgccacat ccatttagtc ggtcattgaa gtaagacacg ttgacattgc cgtattgatc
      661 aacctcagca aagctaagat aggcaactga tacaccattg ttataaataa aatcccatgc
      721 tcgatcatga ggcatgcgca catctgcatt gtaattcata ccaaaatgtt cacgactccc
      781 aacgaatcca ccgaaaatgc caacatctag aatcggttgc acatcatgtt caacacattc
      841 ttcatgcaat aaattagaga gttcattatt gatgccataa ccgatgctaa ttgtatcgcc
      901 ataagttaaa aactgagcag cacgtcggag aatcaacttg cgactattaa aaggtaatgc
      961 gggttcaggt attccatcaa tccgttcttc tccagacaag gctggtaaat aatgactctg
     1021 aattacttgg cggtgattct tttcatcttc tgtgacgtat acataatcga caagatttcc
     1081 tgggataaca acttcattcg gctttagttg atagtcgtca actaaatctt taacttgtac
     1141 aataactttc ccatgattgg ctttcgcgtt taatgcgaca tgataacact cgctcaagta
     1201 agattcttga gttaaataaa tgttaccttg ttgatctgcg tatgttcctc tcagtagtgc
     1261 cacatcaacg ctagggaatg tgtagtgtaa gtatgtttca tcgtcgatag ttactaatga
     1321 aactaaatca tcagttgttc gtgtatttac tttaccgcca ccgtatctag gatcaacagc
     1381 tgtgtttaat ccgattttag taataactcc cggtaataat tgattactct gacgataatg
     1441 agttgcaatg ataccttgtg gcaaaaaata agcttcaatg tcattatttt tcattgcttg
     1501 tgccgttttg gaagaagccg ttaaaatgct cataatgaca cgtttaatca tgcgacgttc
     1561 tataaaatca tctaaatccg gtgcgacacc taaactatgg atatcattcg ctaatataaa
     1621 tgttaaatca ttgggcgtat gatatgtgtc atgttgcgct aacacagcac gtagaacttc
     1681 ggcgggtaag ttggctacag ctaatgctgg taaaccaatc acatcatcat ctttaatgat
     1741 atgttgtaag tcgtgccatg tgatttgttt caagcaagtc acctccatca catttgataa
     1801 aatatagcgt ttttacactt tgtgtaaacc cttacaagaa atataacata acgacgttta
     1861 aaatcaatta gaaatatctt tttattctga taatagacac agtatagaca cattttaata
     1921 gtcgataaca attgtaatat caagggtttg taatgaattg aatatcatta aaatacttat
     1981 ataaaaatat tgctcggaat ataaaaagta acccatgttt atttaaaaaa tgcaaagact
     2041 aattatcatt ttgatgcatc agtaaggcga gggtgatcag cgtacaatta gtggtgtata
     2101 atgtaactga taaaatcatt ggagagttga aggagatgtt aaacatatga gagtcaatgt
     2161 tttacaacat acaccaaatg aaggtgttgg atacataggt gagtgggcga aggaaagagg
     2221 gcacgaagtc tatatttatc atccatatat gaatgatggc gtgctacctc ttgcggaaga
     2281 aacggatatg ttagtgatac ttggtgggcc aatgaatcca gacgatgaga tgccttggat
     2341 tcaatcagag cgccaattaa ttttagtatt gatggcacaa aacaaaccaa tttatggtgc
     2401 ttgctttggt gcacaacaaa tttcgaaagc attaggttat gcggtgttaa aatcaccagt
     2461 aaaggaagta ggttgggatg atgtttactt agaatcagaa atcattccga atataccaag
     2521 tcgattgaag gcactacatt ggcatgaaga tatgtttcaa ataccgcaac aagcagaatt
     2581 attattttcg gggaaccata ttagaaacca aggatttatc atcgatcatc atgtgattgg
     2641 attacaattt cattttgagc ctggtccaga cgatgtcaga gaaatagtac taaatgatgc
     2701 ccaatacatc aatcattcta ttttagagca atcggcggag gacatattag cctttgaggt
     2761 gcctaaacag aataggcagg ccattttccg aatacttgat tttattactc aagcgtaaga
     2821 tgtgagttgt attttaaaat gagtaaaaaa aacgaatgta cacaatcgaa caaagtagtt
     2881 atataaatat atggaaaatc catcaatatt aaattaaaat agttttatta tgaaaagtga
     2941 aagtaggtta gtctatggaa ggtcttaatc atcaaagaaa tacagaaaaa gaagagacaa
     3001 cacaaacgca atcagttgca cctaatacag gtgaagaagg gatgtcatca gcaagtacac
     3061 aatctactaa gacatccgac atacataatg aatctatcga taaacaaatg gaagccaaag
     3121 cgcatgaaac agcgcaaaat acagatttaa aaaatgaagc aagaagttta tttgataatg
     3181 caaccaaatc aatcggtaga ctagctggca atgatgaaag cttaaatctt aatttaaaag
     3241 atatgttttc tgaagtattt aagccacata ctaaaaacga agcagatgaa atatttattg
     3301 cgggtactgc taaaactacg ccagcaattt gtgacatatc agaagaatgg gggaagccat
     3361 ggctcttttc tcgagtattc atcgctttca cagtaacatt tattggatta tgggtcatgg
     3421 cagcaatttt taataacact aacgcgattc cgggtctcat ttttataggg gctttaacag
     3481 taccattatc gggtttgttc tttttttatg aatcaaatgc gtttaaaaat attagcattt
     3541 ttgaagttat tatcatgttc tttattggcg gcgtattttc attactaagt acgatggtat
     3601 tatatagatt tgtcgttttt agtgatcaat tcgaaaggtt tggttcttta acatttttcg
     3661 atgcattttt agtaggatta gttgaagaaa ctggaaaagc gctcattatt gtttatttcg
     3721 tcaataaatt gaaaacaaat aagattttga atggattatt aatcggtgct gctattggtg
     3781 cagggttcgc agtttttgaa tcagcaggtt atattttgaa tttcgcttta ggggaaaatg
     3841 tcccattatt agatattgtc ttcacacgtg cgtggactgc gattggtggt catttagttt
     3901 ggtcagcgat tgttggtgct gcaatagtta ttgcgaaaga acagcatggc tttgaattca
     3961 aagatatatt tgataaacgc tttttaatat tctttttatc agccgttggt ttacatggca
     4021 tttgggatac atctttaact
//
LOCUS       DAMZPP010000185         4009 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMZPP010000185 DAMZPP010000000
VERSION     DAMZPP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.186"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(41..715)
                     /locus_tag="POH73_002595"
     CDS             complement(41..715)
                     /locus_tag="POH73_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISSau6 family transposase"
                     /protein_id="HDI6888504.1"
                     /translation="MNYFRYKQFDKDVITVAVGYYLRYALSYRDISEILRERGIYVHH
                     STIYRWVQEYAPILYQIWKKKNKQAYYKWHIDETYIKIKGQWNYLYRAIDADGHTLDI
                     SLRKKRDNHSAYTFIKRLIKQFGKPQMIITDQAPSTKVAMSKVIKDFKLTPNCHCTSK
                     YLNNLIEQDHRHIKVRKISYQSINTAKNTIKGIECIYGLYKKNRRSLQIYGFSPCRVI
                     SIMLAS"
     gene            810..1187
                     /locus_tag="POH73_002596"
     CDS             810..1187
                     /locus_tag="POH73_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6888505.1"
                     /translation="MDKQVRNTTEIVRLAKQKSQKTREKVDKAISKFSIEGKAINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITSNVVSKPKKSSRSEEILIKTLKRRVME
                     LKKENKKLQNQIQKLYGDLYNKE"
     gene            1425..1790
                     /locus_tag="POH73_002597"
     CDS             1425..1790
                     /locus_tag="POH73_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDI6888506.1"
                     /translation="MTKDMCEVTYIHEDKVNRAKKDLAKQNPMDVAKVFKALSDDTRV
                     KIAYVLSLEGELCVCDVANIIESSTATASHHLRLLKNLGIAKYRKEGKVVYYSLDDEH
                     VKQLVEKAFLYQREVASIG"
     gene            1783..3963
                     /gene="cadA"
                     /locus_tag="POH73_002598"
     CDS             1783..3963
                     /gene="cadA"
                     /locus_tag="POH73_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase CadA"
                     /protein_id="HDI6888507.1"
                     /translation="MDSSAKTLTEDKQVYRVEGFSCANCAGKFEKNVKQLAGVQDAKV
                     NFGASKIDVYGNASVQELEKAGAFENLKVFPEKLANSSMQAVKEDTKAPKEEKIPFYK
                     KHSTLLFATLLIAFGYLSHFVNGEDNLVTSMLFVGSIVIGGYSLFKVGFQNLIRFDFD
                     MKTLMTVAVIGAAIIGEWAEASIVVVLFAISEALERFSMDRARQSIRSLMDIAPKEAL
                     VMRNGQEIMIHVDDIAVGDIMIVKPGEKIAMDGIIINGVSAVNQAAITGESVPVAKTV
                     DDEVFAGTLNEEGLLEVKITKYVEDTTISKIIHLVEEAQGERAPAQAFVDKFAKYYTP
                     IIMVIAALVAVVPPLFFGGSWDTWVYQGLAVLVVGCPCALVISTPISIVSAIGNAAKK
                     GVLIKGGVYLEELGAIKAIAFDKTGTLTKGVPVVTDFKVLNDQVEEKELFSIITALEY
                     RSQHPLASAIMKKAEQDNITYSDVRVEDFTSITGRGIQGNIDGTTYYIGSPRLFKELN
                     VSDFSLEFENKVKVLQNQGKTAMIIGTDQTILGVIAVADEVRETSKNVIQKLHQLGIK
                     QTIMLTGDNQGTAEAIGAHVGVSDIQSELMPQDKLDYIKKMKAEHGNVAMIGDGVNDA
                     PALAASTVGIAMGGAGTDTAIETADIALMGDDLSKLPFAVRLSRKTLNIIKANITFAI
                     GIKIIALLLVIPGWLTLWIAILSDMGATILVALNSLRLMRVKDK"
BASE COUNT         1324 a          634 c          832 g         1219 t
ORIGIN      
        1 aaataagtgt ctaaccttat gtttaaaata atacttcgta ttaactagct agcatgatgc
       61 taattacacg gcatggcgaa aacccgtaga tctgaagaga tctgcggttc tttttatata
      121 gtccataaat acattcaatg cctttgatcg tattctttgc cgtattgata ctttgataac
      181 ttattttcct tactttgata tgacgatgat cttgttcaat gagattattt aaatatttag
      241 aggtacaatg acagttgggg gtaagtttaa aatctttaat cactttggac attgccacct
      301 tcgttgaagg ggcttggtct gtaattatca tttgaggttt gccaaattgt ttaataagac
      361 gtttaataaa cgtatatgct gagtgattat ctcgtttctt acgtaacgaa atatctagtg
      421 tatggccatc tgcatcaata gcgcgatata aataattcca ttgcccttta attttgatat
      481 acgtctcatc tatatgccat ttataataag cttgcttatt ctttttcttc caaatttgat
      541 acaaaatagg agcatattcc tgtacccatc gataaattgt tgaatgatga acatagatgc
      601 ctcgttctct caatatttca gatatatcac gataacttaa tgcatatctt agatagtagc
      661 caacggctac agtaatgaca tctttgtcaa attgcttata tctgaaataa ttcatcctct
      721 gcacctcatt tttgttgact attatactat atttctaact ttgcaacaga accgaacaaa
      781 tcattggaag tttgacgggg taattatcaa tggataaaca agttagaaat acaacagaaa
      841 ttgtacgttt ggcgaagcag aaatcacaaa agacaaggga aaaagtagac aaagcgattt
      901 ctaaattttc gattgaaggt aaagctatta attttaattc aatagcaaag gaagctaatg
      961 tttctaaatc atggctttat aaggaacacg atattaggca aagaatcgaa tcccttcgtg
     1021 agcgtcaaat aacatcaaat gtagtctcaa aacccaagaa aagttctcgt tcggaggaaa
     1081 tccttataaa aaccttaaaa agaagggtta tggaattaaa aaaagaaaat aaaaaattac
     1141 agaaccaaat tcaaaaatta tatggagatc tgtataataa agagtagttt taattattaa
     1201 tctgtagaca gattgtgaaa ggatgcactt aaactaacgg ggcagttatg tttaagatac
     1261 tttaaaattt ggttaaaatc tccgaaagga ttggtttcta acattagaaa tcaatccttt
     1321 ttaatattaa ttatatatca ttcaaacaat catttgacta tttgtttatg gtgggatata
     1381 ttataatcat caaacaaaca gtcatttgaa taaggaggat aattatgaca aaagatatgt
     1441 gtgaagttac ctatattcat gaagataagg taaacagggc taaaaaagac cttgctaaac
     1501 agaatcctat ggatgtagcg aaagttttta aggctctatc agatgataca agagttaaaa
     1561 ttgcttatgt tttgtcttta gagggagagt tatgtgtttg tgatgtagct aatatcattg
     1621 aatcttcaac ggctacggca tcccatcatt taagattatt gaaaaattta ggtatagcaa
     1681 aataccgtaa agaaggaaaa gtagtctatt attcactaga tgatgagcat gttaaacagc
     1741 ttgtagaaaa agctttcttg tatcaaaggg aggttgctag tattggatag ttcagcaaaa
     1801 acattaacag aagataaaca ggtttaccgt gtggagggtt tctcgtgtgc gaattgtgct
     1861 gggaagtttg aaaaaaatgt aaaacaacta gctggagttc aggatgcaaa agtgaacttt
     1921 ggcgcttcca aaattgatgt atatggaaat gcatcggttc aagagcttga aaaagcaggt
     1981 gctttcgaga atcttaaggt atttcccgaa aaactagcga attcatcgat gcaagcggtc
     2041 aaagaagaca ctaaggctcc taaagaagag aaaataccgt tttataaaaa acacagcaca
     2101 ttgctgtttg ccacattact gattgctttt ggttaccttt ctcactttgt aaatggagaa
     2161 gataaccttg taacttccat gttatttgta ggttcgattg taattggcgg atattcacta
     2221 tttaaagttg gttttcaaaa tttgatacgc tttgatttcg acatgaaaac cctgatgacc
     2281 gtagcagtta ttggagctgc catcattggt gaatgggcag aggcatccat tgttgtcgtt
     2341 ctctttgcaa tcagtgaagc acttgaacgt ttttctatgg atagagcaag acagtccatt
     2401 cgttcattga tggatattgc cccaaaagaa gcacttgtta tgcggaatgg tcaggaaata
     2461 atgatccatg tggacgatat tgccgtgggt gatattatga tcgtcaaacc aggggagaaa
     2521 attgccatgg atgggatcat tataaatggt gtgtcggctg tcaaccaggc tgctataaca
     2581 ggagaatctg tccctgttgc caaaacggta gatgatgaag tatttgcagg tacgcttaac
     2641 gaagagggac tacttgaagt aaaaatcacc aaatacgtag aggatacaac tatctccaag
     2701 attattcatc tggttgagga agcacaaggg gagcgcgctc cagcgcaagc attcgtagat
     2761 aaatttgcga aatattatac gccgatcatt atggttattg cggcgctcgt tgcagtcgtt
     2821 ccacctttat tctttggtgg aagttgggat acttgggttt atcaaggatt agcggtactt
     2881 gtagttggat gtccgtgtgc attagttatt tctactccaa tctcgattgt ctcggcaatt
     2941 ggaaatgcag ctaaaaaagg tgtgttgatt aaaggcggtg tctatctaga ggaattagga
     3001 gccattaagg caatcgcatt tgataaaaca ggaacactga caaaaggtgt accagtggta
     3061 acagatttta aagtgttaaa tgatcaagtg gaagaaaaag agctgttttc cattattaca
     3121 gctttagaat atcgatcaca acatccactt gcttcagcaa taatgaagaa agcagagcaa
     3181 gataatatta cttattccga tgttagagtg gaggacttca cttctattac aggtcggggc
     3241 attcaaggga atatagatgg aacaacctat tacattggca gtccaaggct ttttaaagaa
     3301 ttaaatgttt ccgattttag ccttgagttt gaaaataaag tgaaagtttt acaaaaccaa
     3361 gggaaaacgg ccatgattat tggaacggac caaacaatcc tcggcgtgat tgctgtagca
     3421 gatgaggtcc gcgaaacaag taaaaatgtg attcaaaaac ttcatcagtt aggaatcaag
     3481 caaacaatta tgctgacagg tgataatcaa ggtaccgcag aagcaatcgg tgctcatgta
     3541 ggcgtttctg atattcagtc cgaattgatg ccacaggata agttggacta tattaaaaaa
     3601 atgaaagccg agcatggtaa tgtagctatg attggcgatg gcgtcaatga tgctcctgca
     3661 cttgctgcat ccactgttgg cattgcaatg ggcggtgctg gaacagatac tgccatcgag
     3721 acagctgata ttgcattaat gggagatgat ttaagtaagc ttccatttgc agtaagactt
     3781 agcaggaaaa cgctaaatat catcaaagcg aacatcacgt ttgccatcgg aattaagata
     3841 attgccttac tattggttat tccaggctgg ctaacccttt ggattgcgat tctttccgat
     3901 atgggagcca ctattttggt agcattaaat agtttgcgac tgatgagagt gaaggataaa
     3961 taggtactat atgtgtgcaa tttacggaga ggctgggaca taaatccct
//
LOCUS       DAMZPP010000186         3940 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMZPP010000186 DAMZPP010000000
VERSION     DAMZPP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.187"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            206..700
                     /locus_tag="POH73_002599"
     CDS             206..700
                     /locus_tag="POH73_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6888508.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
     gene            856..1473
                     /locus_tag="POH73_002600"
     CDS             856..1473
                     /locus_tag="POH73_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDI6888509.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLFIIIAVVGVSIIIMSLPILQAIIYIVGLIFLMYMAWTIWHDKPSTDGEAQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
     gene            complement(1546..2127)
                     /locus_tag="POH73_002601"
     CDS             complement(1546..2127)
                     /locus_tag="POH73_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDI6888510.1"
                     /translation="MAKTLYLMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGLFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEDRIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            2352..2588
                     /locus_tag="POH73_002602"
     CDS             2352..2588
                     /locus_tag="POH73_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888511.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            complement(2585..2788)
                     /locus_tag="POH73_002603"
     CDS             complement(2585..2788)
                     /locus_tag="POH73_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDI6888512.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            3129..3389
                     /locus_tag="POH73_002604"
     CDS             3129..3389
                     /locus_tag="POH73_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888513.1"
                     /translation="MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICG
                     VILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPIFYSFIG"
     gene            3418..3607
                     /locus_tag="POH73_002605"
                     /pseudo
     CDS             3418..3607
                     /locus_tag="POH73_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3795..>3940
                     /locus_tag="POH73_002606"
     CDS             3795..>3940
                     /locus_tag="POH73_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888514.1"
                     /translation="MNYIKRTLILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIIT"
BASE COUNT         1343 a          579 c          545 g         1473 t
ORIGIN      
        1 cgtctacaaa taaaagtcca gtgtcgacca ataaccaatt ccataattcc ctgaacttaa
       61 aaataaatat aattaaattg ataatccatt tacaagaatg tgcatataaa tcaatgattg
      121 caaaatttta ataaacattt taatatgtaa ttaagaaatt tttttagtac aaaatctttc
      181 ttaataatga ttaaggaggt cagtaatgat tgaaattaaa acattaacga ataatgattt
      241 taatgagtat aagagacttg tttcgacagt caatgaagaa ttcactcaag attcacatta
      301 tagtcaaaca atgactgaca ccttaataca tgacatttta aatcaaggtt caccgaaatg
      361 tattgtattt ggctgttatg aaaacgaaac acttatcgca acagctgcct tagaacaaat
      421 tcgatacgtt ggaaaagaac ataaatcatt aattaaatac aactttgtta ctaataacga
      481 taaatcgatt aatagcgagc tcattaattt cattattaat tatgcacggc agaacaatta
      541 cgaatcttta cttacatcaa ttgtgtcaaa caacataggt gctaaagttt tctatagtgc
      601 actaggattc gacattcttg gttttgagaa aaatgcaatt aaaatcggaa atacctattt
      661 cgatgaacat tggctttttt atgatttgat taataagtaa tacagtttta tatattctac
      721 atttctcata aattcaaatc atatattaat ctatttttca tacaactact ctactattga
      781 gtagttgttt tttatttggt atgatatcaa taagtttttt cagaatattc tatgttttag
      841 gggtgtcaaa acttaatggt aaccgcaatt attcatggat ttatcttagc gattggtcta
      901 attttacctt taggtgcaca aaatgtattt atttttaacc aaggagctaa tcaaccaaaa
      961 tatagatatg tattgcctgc aataattaca gccgggttgt cagacagctt atttattatt
     1021 attgcagtgg taggagtttc tatcattatt atgtctttac ctatacttca agcaattatt
     1081 tatatagttg gtttaatttt cttgatgtat atggcttgga ccatttggca tgataaaccc
     1141 tcaacagatg gagaagctca aattatgtct ccaatgaaac aagtaagttt tgctttatca
     1201 gtttcattac tcaatccaca tgctatttta gatacaattg gtgtaattgg tagtagtgct
     1261 gcattatata gtggcagcaa taaaattgcg tttacaatcg cttgtattag cgtttcatgg
     1321 ttatggttct ttttactcgc aattttagga aaaatggttg gatcaattga taaaaccgga
     1381 aaattattaa caataatcaa taaaatatca agcataataa ttattattgt tgctctgatg
     1441 atattacaaa aacttattca attattattc taaagcgtca tgtagttgta ttttaaaagc
     1501 gttatacaaa agacatactt gtgtctatgt ataacgcttt atttattata aatttggatc
     1561 aattaactca acaaatttaa atgttccatt gtcatattca aatttcaaga tattacaatt
     1621 accgatatta tgctttaatg cttcatctag tttaaggcaa tatcttaaaa atactcccat
     1681 tattgttcca tgactcacga ctaatgcatt attctttgtt tgagacataa tgtcatgtaa
     1741 agttttcaca atgcgatctt caacttgttg ccttgcctct cctttgaaag gaacaattcg
     1801 atctccgaat aagtcttcag gcttgtatag attatcaaat agatagactg actcaccttc
     1861 aaataatcca aaatgccatt ccttcagtcc cttaaaacgt tgatacgatt gattaggtgc
     1921 aacattttca agtgtgtcac ttgcacgttc ttgcgttgat gatgcatata aatcgaagtt
     1981 tatcccctta gtttcataat agctacgtgc cttttgagct tgagcaattc caagttctgt
     2041 tagcggcgaa tctccaaatc cctgaattag tcccttaaaa ttaaacaaag tttgtccgtg
     2101 gcgcattaaa tataacgttt tagccaacaa taacacctct tcaataaaaa gtattgatag
     2161 ctcaaaatgt attctctttt tattaaaatg agtaccaaat atattaaacc attatttaaa
     2221 ctttttcggt agcgaaaacc tttgcttttg atgaacattt ttttcataag taatgatgat
     2281 tttagtttaa aagataatgt gacggggtaa aacgcaatga agagtacaga attttaaagg
     2341 agtgagtgtt tatggataat cataatcaat gtaattatgt gaatccacaa aatgtttcac
     2401 ttgactggga atgttttata attagtaaaa gtgaaatgtt gttagatggt gtaccaaatg
     2461 aacttataaa cacttggtta gataaagaca ttattacacc attttcgata agaaatgatg
     2521 aaataaactt taaaacaaaa gatatttggg atgcactaat acatcataat tggtactact
     2581 caaattaatt tgccatatat aagctaatag ctctatttgt agtttcaatt aattgatgat
     2641 ctgtgtaatt agacatcaca tatgctctaa ttacttggtg tctttcttct aatcgaggga
     2701 atgctttatc ttcactaacg tgttcggcca atataccaaa tggtgtgtta tcattttgaa
     2761 aacccatgat gaaattgtaa aacgtcataa gacttgcccc cttcatttat taaacgctat
     2821 ttaaagtgtt attattaatc ttatcagaaa actagtattt gtaaatgtgc aaaagtgatt
     2881 ttgcacattt ttttgttatc tgattctaaa aatattaacg aattgtacaa ctttaagggt
     2941 ttttagtatg taaatattct tttcacatta atactatact cccctatata tgctcacttg
     3001 tttgaattaa taaactttag cattctacaa ataatatttt aaaaataact atacttttta
     3061 tttacctatt ggaaaatagt gttatcatat ttattaagaa gctattttta tactagtagg
     3121 tgtcagtcat gaataacatt ttgttaaatg ctatcaatat agttattact accacttttg
     3181 ttatctttaa tattttaatc acatataata aagatttaga tgatttatgt tggctcctgc
     3241 ctggtattat catttgtggt gtgatactca tagtatcctt taccattgca atgataacta
     3301 aaaactggtt aagtgaaata ttatttttta taaatatcgt actcgttctc tattacattt
     3361 atcctatttt ttatagtttt ataggttaat aattacggta tataaggagg gtttcacatg
     3421 tctttacatt ttgcaattct gttttggcta gcattaattt tcttagttgc cgctacgttt
     3481 atactcgtat taatgaaaaa aaactggcaa agaatctaaa aaagagtcct atttaagttt
     3541 cactgtcatt ctctatattt tcggattcgc tattttaata tacacattta tatttggtgt
     3601 gctataaatg aaattcattt tatatccagt ctgaaacgtc tcaaagatgt tttagactgt
     3661 tttttattct taatagacat ttcacactct catactttaa tatttaagat atgttttagt
     3721 cttgcatgct tattaaagat taaaattata attcaaggct atttacatat atttcttcaa
     3781 caaggagctt ttaagtgaac tatattaaac gaacgttaat tttacttata ttattcgttg
     3841 tcgtatcccc tataaactca ccaaaaacaa ttgcagataa taagtattca gaaattcaag
     3901 atgacaaatt ccaattacaa cctggtgata ttattattac
//
LOCUS       DAMZPP010000187         3889 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMZPP010000187 DAMZPP010000000
VERSION     DAMZPP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.188"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            232..1017
                     /locus_tag="POH73_002607"
     CDS             232..1017
                     /locus_tag="POH73_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HDI6888515.1"
                     /translation="MKVQIYQLPIIFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     KLINEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
     gene            complement(1620..1754)
                     /locus_tag="POH73_002608"
     CDS             complement(1620..1754)
                     /locus_tag="POH73_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HDI6888516.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
     gene            1990..2553
                     /locus_tag="POH73_002609"
     CDS             1990..2553
                     /locus_tag="POH73_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HDI6888517.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTLAYILNIFIFTLITNISFYLIRRYAHGAHAPSSFWCYIESITLFIVLPLLVLHF
                     HINETLMMFLALLSVGVVIKYAPAATKKKPIPARLVKQKRYFSIIISTILFIITLFVK
                     EPYTQFIQLGIIIQAITLLPIYYSKED"
     gene            2557..2697
                     /locus_tag="POH73_002610"
     CDS             2557..2697
                     /locus_tag="POH73_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDI6888518.1"
                     /translation="MKKLLNKVIELLVDFFNSIGYRAAYINCDFLLDEAEVPKELTQL
                     HE"
     gene            2722..>3889
                     /locus_tag="POH73_002611"
     CDS             2722..>3889
                     /locus_tag="POH73_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDI6888519.1"
                     /translation="MEALNDYNYVLFVIVQVSLMFFISAFISGIRYKKSDYIYIIGIV
                     LSSVYFFDKIRSISLVVITIFIIIFLYFKIRLYSVFLVMVTQIILYCANFVYIIIFSY
                     IITISHSVFIVLPIFLVVYVSISYALAYILNRILKRINGTYLSLNKKFLTVITIVIVI
                     TFSLLFAYSQIDASDASTIKQYSLLFLGIIILLSILIFIYSQFTLKEMKYKRNQEEIE
                     TYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMTGLRDYFNKNIVPMKDNLQ
                     MNALKLNGIENLKVREIKGLLTAKILRAQEMNIPISIEIPDEVTRINLNMIDLSRSIG
                     IILDNAIEASSEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTK"
BASE COUNT         1357 a          541 c          575 g         1416 t
ORIGIN      
        1 taagaaatac taatttctaa agaaaaagta tttctttacg ttagagccca ccccaacttg
       61 cattgtctgt agaaattggt gttccaattt ctctatgttg gggccccatc aactactgtc
      121 aatataacat tgtagagcct aggactttga ttttatgtcc tgggcttgtt ttttgcgttt
      181 caacataact tttagtatgt tatatataag atctatcaag gatgtgatgt tatgaaagtc
      241 caaatttatc aattaccgat tatttttgga gatagttcta aaaatgaaac tcaaataacg
      301 caatggttcg aaaaaaatat gaatgctgaa gtagatgtag tcgttttacc tgaaatgtgg
      361 aataatggct atgatttaga gcatttaaat gaaaaagcag ataataatct aggacaaagt
      421 ttttcattta ttaaacattt agctgaaaaa tataaagtcg atatcgtagc tgggtcagtt
      481 tctaacatta gaaacaatca aatatttaat acagctttta gtgtgaataa aagtggcaaa
      541 ctgattaatg aatacgacaa agttcattta gttcctatgt taagagagca tgaattttta
      601 accgctggcg aaaacgtagc tgaacccttt caattgtctg acggtactta tgtgacccaa
      661 cttatctgtt atgatttacg ttttccagaa ttattaagat atcctgctcg tagtggtgct
      721 aaaattgcat tttatgtggc acaatggcct atgtcacgtc ttcaacattg gcattcttta
      781 ctaaaggcac gtgcaattga aaataatatg tttgtcattg gaactaatag cactggattc
      841 gatggtaaca cagaatatgc tggtcattcg atagttataa atccaaacgg agatttagtt
      901 ggagaattaa atgaatccgc agatattttg actgtagatt taaacttaaa tgaagtagaa
      961 cagcaacgcg aaaatatacc tgtatttaaa agtattaaat tagatttgta taaataaaaa
     1021 gcacacgaaa taatatttaa cacatgtcat gactaaacat agatttatga gacgattttt
     1081 cataagccta tgtttatttt tttataatag ataactcagt aagaaccagt ttcgccctta
     1141 gcaaatgaat gattttctta attaaattga ttattttttt gaccttttcc aacattagac
     1201 ttattcatat attttaacgg tggatctcat aatgaggatt aactcttccc tactttatta
     1261 caataaaaaa aggccgcgag cttgggaggg gctcacgacc atacttatta ttaagggaat
     1321 gttttacagt tattttttca atctattttt ggggatgtta ttaattatga aaaaaaattt
     1381 tatttatcaa ataatgataa atcgatgttg tttacgatag cttacatgct agaaataatt
     1441 atctgtaatg ataattaaga aaaatacata gcactgagtc caaggaaact aactctacta
     1501 gcaaatgtta ctcacttgct aattttacat ggttattaag ttgggatggc ttaataactc
     1561 atacttttag tactatacga agataacaaa tttacaatga aagtaattaa ttattcatct
     1621 tattttttag tgaatttgtt cactgtgtcg ataatccatt ttactaagtc accgattgtt
     1681 gaaatgatat cttgtgccat tgaaatcact ccttccttaa ttaagataaa aattcttaaa
     1741 attaaacaac tcatcaacta ttttccatca catctctgtg atctagttat attaaaacat
     1801 gctataagca tttattttcc attttttctt aactagtcgt tttttattct taactgtaaa
     1861 tttttttatg ttaaaatatt aaatacaaat tacatttaac agttaagtat ttatttccta
     1921 cagttaggca atataatgat aaaagattgt actaaatcgt ataataacag tgaggagagt
     1981 ggtgtaaaat tgaattattt tgataacaaa attgaccagt ttgccacgta tcttcaaaag
     2041 agaaataact tagatcatat tcaatttttg caagtacgat tagggatgca ggtcttagct
     2101 aaaaatatag gaaaactgat tgttatgtat actcttgcct atattttaaa catttttatc
     2161 ttcactttaa ttactaatat atcgttttat ctaattagaa ggtatgcaca tggtgcacat
     2221 gcaccttcat ctttttggtg ttatatagaa agcattacac tttttattgt tctacctcta
     2281 ttagtactac attttcatat taatgaaaca ctaatgatgt ttttagcatt attaagtgtt
     2341 ggcgtagtga ttaaatatgc acctgcagct actaagaaga agcctatccc ggcaaggctt
     2401 gtaaagcaaa aaagatattt ttcaattata attagtacaa ttcttttcat tatcacactt
     2461 tttgtaaagg aaccatatac ccaatttatt caactgggta ttattataca agctattaca
     2521 ttactaccaa tatactattc aaaggaggac taaattatga aaaaattact caacaaagtt
     2581 attgagcttt tagttgactt tttcaacagc atcggttaca gagcagccta tataaattgt
     2641 gattttttat tggatgaagc tgaagtacca aaagaattaa ctcaattaca cgaataacta
     2701 taagagaaag tgtgatagta agtggaagca ttaaatgatt ataattacgt tttatttgta
     2761 attgttcaag tttcattaat gttctttata tctgcattta ttagtggaat acgatacaaa
     2821 aaatcagatt atatatacat tattggaata gtgttatctt cagtatattt ctttgacaaa
     2881 atcagaagta tttcactagt tgttataacg atttttataa taatttttct ctattttaaa
     2941 attagactat attctgtatt tttagtaatg gttactcaaa tcattttata ttgtgcaaat
     3001 ttcgtttata tcattatttt ttcatacatt ataacaattt cacacagcgt gtttatagtt
     3061 ctaccaatct ttttggtagt gtatgtatca atttcttatg cacttgccta cattttaaat
     3121 agaatattaa aaaggataaa tggtacatac ctttctctta ataaaaaatt ccttactgtt
     3181 ataactattg ttatagtaat aacattctct ttgttatttg catattcgca aattgatgcg
     3241 agtgatgcat caacaatcaa acagtattcg cttttattct tgggaattat tatattactg
     3301 agtatcttaa tttttattta ttctcaattt actcttaaag aaatgaaata taaacgtaat
     3361 caagaagaaa ttgaaaccta ttatgaatac acattaaaga ttgaagcaat taataatgaa
     3421 atgcgcaagt tccgtcatga ttatgtcaat atcttaacga ctctttcaga atacattcgt
     3481 gaagatgaca tgaccggtct tcgagactat ttcaataaaa atattgtacc tatgaaagat
     3541 aatttacaaa tgaatgctct taagttaaat ggtattgaga atcttaaagt acgtgagatt
     3601 aaaggtcttc taacagctaa aattttacgt gcacaagaaa tgaatatacc gattagtatc
     3661 gaaataccag atgaagtaac tcgcattaat ttgaatatga tcgatttaag tcgcagtatt
     3721 ggtattattc ttgataacgc aatagaggca tcaagtgaaa ttgatgatcc tatcattcga
     3781 gttgcattta ttgaaagcga aaattcagta acgtttattg ttatgaataa atgtgcagat
     3841 gatataccac gtattcatga attgttccaa gaaagttttt ctactaaag
//
LOCUS       DAMZPP010000188         3873 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMZPP010000188 DAMZPP010000000
VERSION     DAMZPP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.189"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..119
                     /locus_tag="POH73_002612"
     CDS             <1..119
                     /locus_tag="POH73_002612"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDI6888520.1"
                     /translation="MQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            141..1157
                     /locus_tag="POH73_002613"
     CDS             141..1157
                     /locus_tag="POH73_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDI6888521.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDGDYN
                     NFIMVSYIKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMAKNHYFDHTNLKGQSPFDRLKKDG
                     IAFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTD"
     gene            complement(1537..2160)
                     /gene="lexA"
                     /locus_tag="POH73_002614"
     CDS             complement(1537..2160)
                     /gene="lexA"
                     /locus_tag="POH73_002614"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDI6888522.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            2303..2536
                     /locus_tag="POH73_002615"
     CDS             2303..2536
                     /locus_tag="POH73_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888523.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANVSAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            2672..2911
                     /locus_tag="POH73_002616"
     CDS             2672..2911
                     /locus_tag="POH73_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDI6888524.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            3032..>3873
                     /locus_tag="POH73_002617"
     CDS             3032..>3873
                     /locus_tag="POH73_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDI6888525.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEDERKLTFEN
                     YGLDP"
BASE COUNT         1446 a          543 c          656 g         1228 t
ORIGIN      
        1 aaatgcaaca agatttacaa agttcaatct cttatgccgg tggaaaagac ttgaaatcat
       61 tacgtactgt agattatgtt attgttagaa actctatttt caacggtgat agagattaat
      121 atttatagta ggtgatgtaa attaaaaaat tcatagtaac tgttgttgca tttttatcaa
      181 ttattatcat tgcgccaata acagaattta aaccattcat tcatttacaa aatgaagtaa
      241 gacaatatat tgacattcac atcaataaag aaacaatttc tgcggaaaat aaattggata
      301 cgccgaagaa acaacaattt gcctttaaca atatacaaat gaacatgtcg aaatcagatg
      361 ttgagaaaac attaaataaa ccaaaaagag tgacatttaa tgaatatggt acgaagtggt
      421 atacgtatta tgatggtgat tacaataatt ttatcatggt aagttacatt aaagataaag
      481 ttaatgcgtt atatacaaat caaaatatca tcacttcaaa atcaaaaatt aaatacaata
      541 caccgaaatc ggttgtaagg caaagattag gtgaaccaga aacagagatt gttaaaggta
      601 gagtgcgtta cgaacaaaat aataaagaat atgatgtttt ccataaaaat cacatttata
      661 cgacggtatt ttatgataag catcgacgta ataatgtaac agctgtttta caagtaagtg
      721 atgctatgga aaatagatta aaagaacaat atggagcacc ttcgaaatcg cttgcagata
      781 gttttgaact acaaaatttt gatttagtta atgctgaaag aaaacaacat caattatcta
      841 cattgaagta ttctaaacag aattctgaaa ctgctcgtaa gcatagtaaa gatatggcca
      901 aaaatcatta ttttgatcat acaaatttaa aaggtcaatc accatttgat cgattgaaaa
      961 aagatggtat tgcatttaac tcagccggag agaatttagc atatggtcaa gttagtagta
     1021 tctatgcaca tcaaggatta atgaattcta ttggtcacag aaaaaatatt ttaaatgata
     1081 cgtttaaaat attaggcgtt ggtgttgatt ttaatgatga aaaacaacct ttttggacag
     1141 aaaattatac tgattaatgt ttttggcatg agattaaagg gtaatgtttg tctgtaagga
     1201 agtagataaa catgatgaca acagataaac caaaagatgc ggatatactt gaacgcgtca
     1261 aagatatact taataaaaaa gaaagaaaaa aataataatg gctcgctctt gtaaattatt
     1321 acgggggcga gttttaattt gtgtaacatt ttgcaggaat ctgtaattca aaatttatat
     1381 taaagaatga gattatctaa caattcgcaa tttctgtttg aactgaattt agattagaat
     1441 aaataaaacc tcttgaaaac gtaacttttt caagaggtcc aaaatttata aaaatgaggg
     1501 cttaacaatt acgataatat ataacggttt aaaatattac atttcgcggt acaaaccaat
     1561 tactttcccg attacagcaa cattgtcgag gtaaattggc tccattgtac tattttcagg
     1621 ttgtaatcga taacgatttt tttctttata gaagcgtttg acagttgctt catcttcctc
     1681 agtcatagca acaataatgt ctccattttc tgctatggtt tgactgcgaa caattacttt
     1741 gtctccgtct aatataccag cctcaatcat actgtcgcct acgacgttta atatgaatat
     1801 gtcgctattg tgtgtcgatg ttaagtgttc aggtaatgga aaatattctt caatattttc
     1861 tactgcggta ataggaacac ctgctgtgac tttaccaata actggcacat gaatcgtttc
     1921 ttccatatta atattatcat ttgtttgatc acttacaatt tctatagcac gtggtttcgt
     1981 tggatctctt cttatatagc ctttttcttc aagacgtgaa aggtgaccat gaacagttga
     2041 actggatgct aagccaactg cttcaccaat ttcgcgaaca ctaggcggat aacctttcgt
     2101 ttgaacaact tgtttaatat agttatatat ttcgctttgt cgttttgtta attctctcat
     2161 atataggcac tccctaaatt aatttaaata cagtatatca ttaattcaca accaaaacaa
     2221 acatttgttc gttaaaatat tgacacagaa cataagttct gataaattac taatacaaac
     2281 aaatgttcta ggagtgaaaa tgatgtttta caataaatat aaaaacgtat caacatatat
     2341 catcatattt ttagtttcaa gtgcagcctt tgcaatattc ttgttaagtg cgaacgttag
     2401 tgctcactcg gaacaagtgt acgaaatgac tgaccatcaa attaagaaca atacgataaa
     2461 taaagcatac gaacataaag accctacaaa caatagcgaa caaagagatg ggaaagtgtt
     2521 cgctttaata aattgataca ttgtcacaac gttattttgc ctatttttgc gaaatagcgt
     2581 tttttattac ttttttgctg atcttaaatt tgttatattt tgttaaagta ttataatgat
     2641 tgaataaaca aattgaaggt aggtttttta attgagtaat tctgatttga atatcgaaag
     2701 aattaacgag ttagctaaaa agaaaaaaga agtaggatta actcaagaag aagcaaagga
     2761 gcaaacagcg ttaagaaaag cttatcttga gagttttaga aaagggttta aacaacaaat
     2821 tgaaaatact aaagtaattg atccagaagg taatgatgta acacctgaaa aaattaaaga
     2881 gatacaacaa aaaagagata ataaaaatta aaacacaaat ctgtaaagaa ttttctgaca
     2941 ttataacttg aaataagtat tttacttatc tttttatttt aaaataagtt ataatgtatt
     3001 tgataaaatt gaagaaggga agatacacaa gatgtttaat gaaaaagatc aattagctgt
     3061 tgatacgcta cgtgcactaa gtatcgacac aatcgaaaaa gcgaattctg gtcatccagg
     3121 attacctatg ggagctgccc caatggctta cactttgtgg acacgtcatc tgaattttaa
     3181 tccacaatct aaagattact tcaatagaga ccgtttcgta ttatctgcag ggcatggttc
     3241 agcactattg tatagcttgt tacatgtttc tggtagttta gaattagaag aattaaagca
     3301 atttagacaa tggggttcta aaacaccagg tcatcctgaa tacagacata cagatggtgt
     3361 agaagttact actggaccac ttggacaagg ttttgctatg tcagttggat tggctttagc
     3421 agaagatcac ctagcaggga aatttaataa agaaggatat aatgttgtag atcattacac
     3481 atatgtatta gcttctgacg gtgatttaat ggaaggtatt tcgcatgaag cagcttcatt
     3541 tgctggacat aataaattaa gtaaattagt tgttttatac gattcaaatg atatttcatt
     3601 agatggcgaa ttaaacaaag ctttttctga aaacacaaaa gctcgttttg aagcatatgg
     3661 ttggaattac ttactagtta aagatggtaa tgatttagaa gaaattgata aagcgattac
     3721 tacagctaaa tctcaagaag gaccaacaat tattgaagtt aaaacaacaa tcggatttgg
     3781 ttcaccgaat aaagcaggaa ctaatggtgt tcatggggca cctttaggtg aagatgaaag
     3841 aaaattaaca ttcgaaaatt acggtttaga tcc
//
LOCUS       DAMZPP010000189         3811 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMZPP010000189 DAMZPP010000000
VERSION     DAMZPP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.190"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(117..995)
                     /locus_tag="POH73_002618"
     CDS             complement(117..995)
                     /locus_tag="POH73_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HDI6888526.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLNQSKIGGMPFLKSFKDIPLDENNVPMVLLAQINLNDLPEQQELFPVNEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLKDIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDDLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
     gene            complement(1173..1553)
                     /gene="gcvH"
                     /locus_tag="POH73_002619"
     CDS             complement(1173..1553)
                     /gene="gcvH"
                     /locus_tag="POH73_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDI6888527.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQLEALLTAEKYSEMIGE"
     gene            complement(1711..2067)
                     /locus_tag="POH73_002620"
     CDS             complement(1711..2067)
                     /locus_tag="POH73_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDI6888528.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            2211..2531
                     /locus_tag="POH73_002621"
     CDS             2211..2531
                     /locus_tag="POH73_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI6888529.1"
                     /translation="MQAIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            complement(2681..3220)
                     /locus_tag="POH73_002622"
     CDS             complement(2681..3220)
                     /locus_tag="POH73_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDI6888530.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEEDMPKAPRKNRNLITLY"
     gene            complement(3303..>3811)
                     /locus_tag="POH73_002623"
     CDS             complement(3303..>3811)
                     /locus_tag="POH73_002623"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDI6888531.1"
                     /translation="TKLQGGYGQFINDLYLNLISDLANINGIDMIDIEWQADIDIEKH
                     QRIITHLQQYNKEVIISHHNFESTPPLDELQFIFFKMQKFNPEYVKLAVMPHNKNDVL
                     NLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIGEPQAPGQIDVTD
                     LKAQVTLY"
BASE COUNT         1211 a          701 c          495 g         1404 t
ORIGIN      
        1 atgaaaaaga accattacat tataaatcac tagtggttct ttatcatttc tgtcccactc
       61 cctagattga tctacagata ctacacttat taaagtaata tatttttatg attctcttag
      121 ctgcaatccc atgaatacat gtaatcatca aacttcatag cctcaaggtc agtagatttt
      181 actaagatat tggcaatacc taaatcaccc catataatcg aagaattacc atctcttgta
      241 gaatcaattt gcagtaagac tctatcatac attttcagtt cttgatcgtt cgttcttgga
      301 tctatttggt caaaatatgg ataaccacca agttgattaa atggttcctt agcagtaaat
      361 gtgttacaca caggttccat taattcatca taaccaccaa acattgattt tagcgcgaag
      421 gatggattga ctttattcca caatgcaaga tcgtcctcat cgtacttata atcagtacat
      481 gtaatagttt gtttcgacaa tctaaattct atagaaaatg ctccgctgaa cgggatatcc
      541 tcattatcag catctaatga cttcaaatgc gcttgaatat ctttgagtga taaatctgta
      601 attggctctt ttatataaac aagccttgag tttatattgt ttccctttaa attttcagac
      661 ataccataca tttgatcttc tgaactaatc caaaactgca atatcccttc atttacagga
      721 aataattctt gttgttctgg aagatcattc aaattaatct gtgctaacaa taccattggt
      781 acattatttt catctaatgg tatatcttta aaagatttta aaaatggcat tccaccaatc
      841 ttactctggt ttaatgtttc ttcttgcatc tcttttagat ccatttcaat acatggtagt
      901 actggtaaat taccgttttc tacagattct acgaaacggt tatatgcttt ttgctccttt
      961 ttagaaagat gactaacgtc ttgaatgttt agcatattta ctcccccaat ttttattttt
     1021 aaaatagcta aattcattat tttcatcatt cttgaataat aaaatgtagc aatactccaa
     1081 ataatagtac ctaataaatt ttctttcata ttatttactt gctcagacta aaaataaact
     1141 cctcaatctt agattaagga gttacacggt gattattcac caatcatttc tgaatatttt
     1201 tcagctgtta ataaagcttc aagctgactt tcatcactaa tttctacttt taccatccat
     1261 gctttttcgt atggagattc atttacaaat tcgggactat cttctagttc ttcgttgact
     1321 tcaactactt taccagagat tggtgcatat aattctgata cagttttaac tgattctacg
     1381 ctaccaaacg tatccccttc attaatttca tcatctgttt ctggtaattc aacaaaaaca
     1441 atatcaccta actcgctttg tgcgtattct gtgattccaa ttgttgctac attaccttca
     1501 actttaaccc attcatgctc ttttgaatat ttcaattcat ttggtactgc caagagaatc
     1561 ccctcctaat taatctttac atcattaatg atgccataag gctagatgct attcaactat
     1621 atttataaaa attaacaatt gtatagctta tttcatttta catgtaatga atctaaatat
     1681 cgagatattt agtaaacgtt tacattatac ttacgctaac caagtctctt tatattgatc
     1741 ttctttaaat cctaatgtta tcttatcgcc cattactgct agaggacgct ttactaacat
     1801 accatcagat gataacaact ctaacttttc atcatctgat aaagtttgta atttattttt
     1861 caaatcaagc tcacgatatt tcgcaccgtg tgtattaaac aatttattaa tttctacgcc
     1921 tgtatttgca attattgttt taaattcatt tattgtaggt gtatgttgaa cgatatcaat
     1981 tggttcataa cttacgccat attcatctaa aaactttgct gcctttttac aagttgtaca
     2041 attcttatat tggtaaaatt taatcatacg actgcaacct cctttatgta tttaaaataa
     2101 atatatcatt ttccatagca aaaggctttc attttgaagc taaaattttt tattatgata
     2161 taaatttagc tataataaaa attattaaac tatctagagg agttgttaca atgcaagcaa
     2221 tcaaaagtaa tgaatcattt aaatctgtaa ttaatagcga tacacctgta atcgttaaat
     2281 ttgaggcagg atggtgccca gactgtcgtg ctatggattt atggattgac ccaatcgtag
     2341 aacaatataa tgattaccaa tggtatactg ttaatcgtga tgaattagaa gatgtagttg
     2401 ttgaaaatga agttatgggt atccctagct tgctagtatt taaaaacggc gacaaaattg
     2461 cacaccttca ttcagcaaat gctaagtcac ctgaacaagt tgaatcattt ttagcagaaa
     2521 cttttaaata aatactaaaa tacaaatgtg tatatactgc acaataaaaa gctgagactg
     2581 tgattgtacc gactctagta agttagatta aatctctaac tcgatagatg tccatcaatt
     2641 catgactcag cttttattaa tgtttctata attatacata ttaatataat gtaattaagt
     2701 ttctattttt acgtggtgct ttaggcatat cttcttctaa atccgttaaa tataagaatc
     2761 cagcaagaac ttcatctttc tttataccaa gtgtttttct tactttagga tcatagatat
     2821 ataatggcga tttccaacat gtacctattc ccgcttcata aagtaacaac ataagatttt
     2881 gtgcatatgc accaaatgca aagtagtttt catcattttg acgttgtctt ggatctgttt
     2941 ttaaaataag caataacatg ccacctagtt tcgttactgc atcataatga cattgttgct
     3001 tatctaattc attaggaaat gcaaatttag aaatatcctt actcatatct cctaatctgt
     3061 ctttcggaac atgcacaact ctccatggtt ccctcattcc gtgatttgga gcatcagcag
     3121 ctttctcaat tgcttgatat agcaatgcgt catctatgtg catatctctt ttaaattttt
     3181 tcacacttct tctattagct attgcttgtt gtaattccat ctaaaatcgc tcctccacga
     3241 acaatgataa tacttctcaa ttataagaca gcacttacac aaaagcaacc taattcattt
     3301 tattagtata aagtcacttg tgcttttaaa tcagtaacat caatctgtcc tggagcttgt
     3361 ggttctccga tacaaccata agtcaacgca ccaccaaaaa cgccttgagc cgttctactt
     3421 attagtccaa gtttagacat tgaaatacca acaactttgc agtccatagt atctgaaaat
     3481 gtagacattg cctgcaataa atttagcaca tcatttttat tatgtggcat tactgctaat
     3541 ttaacgtatt ctgggttgaa tttttgcatt ttaaaaaata taaattgcaa ttcatctaat
     3601 ggaggcgtac tttcgaaatt atgatgtgat ataatcacct ctttattata ctgttgcaaa
     3661 tgtgtaatga ttcgttgatg tttttcaatg tcaatatctg cttgccattc tatatcaatc
     3721 atatctatgc cattgatatt tgctaagtct gatattaaat taagatacaa gtcatttata
     3781 aattgcccat agccaccttg taactttgta c
//
LOCUS       DAMZPP010000190         3800 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMZPP010000190 DAMZPP010000000
VERSION     DAMZPP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.191"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            315..1805
                     /locus_tag="POH73_002624"
     CDS             315..1805
                     /locus_tag="POH73_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI6888532.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPVQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKLDVEKRINHTIPVVNIPVGYSETIDTPVQTLDQHSVKLISVARYSPEK
                     QLHQQIELVKRLVPYVPKVELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPGELITSDFNGYLITKNDED
                     ALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(1931..3403)
                     /locus_tag="POH73_002625"
     CDS             complement(1931..3403)
                     /locus_tag="POH73_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI6888533.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDLFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFIMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGTGNDIKNFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKDKNHIISIARLVENKQITH
                     QIEVIKQLVTKHPKIQLNIYGHGNGLSEYRQLVEGYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPPGDINQMVE
                     KVDQLLNNSQMMQQFSINSIESAQQYNATTISTKWQKILN"
     gene            complement(3522..>3800)
                     /locus_tag="POH73_002626"
     CDS             complement(3522..>3800)
                     /locus_tag="POH73_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6888534.1"
                     /translation="SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPVKPMSA
                     TKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1231 a          585 c          579 g         1405 t
ORIGIN      
        1 gatgctttcg ctaaattaaa gtatcgcaaa taatatcata tgattaattc aaacgcgtaa
       61 cagtagttgc gcgtttttat tatacctcct agagttgtat atctctttta taaatcttct
      121 catttgatgt gcctgagtca taaatcaata tcctaagctc tacaatgata tattggtagt
      181 atttgactga ataactacta ccattataaa tataaaaatt tgttgtttta taaataaaca
      241 catcttctag ttaaaagaag taattaaata ttatgtgata taattataaa aaatagatat
      301 aagtggtgtt gtaaatgaat tattttgtag gtaatagttt aggtgttaat ttaactggga
      361 tagaaaaagc aattattaat cgtttaaatt tatttaaaga aatgggaaga ccagtacaat
      421 gtgtatttct gtcatggaat agatatttat atagaaatgc ccaaaattat attactagct
      481 ctgactatat caatatgtat gatttcttcc aagaagcaac atatttagaa cgcaatgaac
      541 catttgactg gttgtcatat tggactgatg aatgtcatta tactttaaaa catgttgaaa
      601 atagtcatga ctttagaata tatgatcaag agcgtttctt aatgtatgcc cattttcaag
      661 atcctaaata tcgtatatta gattatgtga atcattttga tagtcaaaga cgaaaagtaa
      721 aacgtgattt ttatgatgtg cgtggctttt taagttgtag taggattctt gttgataaac
      781 aacaaacatt atgtgaattt ttctataatc cagaaggtga cacaaaatta gaaaagtatt
      841 tttcttacaa agatggcaaa ccagaagtac aaaaaattat tgtttactat gctaacaaac
      901 aatatttctt taataatgaa acggaattag gcgcattttt cattaaacaa ctatatcaac
      961 atggggattt atttttcagt gaccgtaatg tgtatacagc acctattttc aacttaacac
     1021 ctgagtcaat cccagtagta gctgtgcttc acagtacaca cattaaaaat attgatgcat
     1081 tagattcatc gccatttaaa aatgtctata aagcgatgtt tgaaaattta tctcgatatc
     1141 gtgccattat cgtttctact gagcagcaaa aattagatgt tgaaaaacgt attaaccata
     1201 ctattccagt tgttaatatt cctgtcggct atagtgaaac aattgataca ccagtccaaa
     1261 cattagatca gcacagtgtt aaattaattt ccgttgctcg ttattcacct gaaaaacaat
     1321 tacatcaaca aattgaatta gttaaacgct tagtccctta tgtaccgaaa gtagaacttc
     1381 atatgtatgg ttttggttct gaaagtaaaa aattaaatga attgatacaa aaatacggat
     1441 tggaaaatca tgtttattta cgtggctttt taagcaatct agaccaagaa tacagcgatg
     1501 cttacttaag tttaataacg agtaacatgg aaggtttctc gttagcgcta cttgaatctt
     1561 tagcacatgg tgttccagtt attagttatg atattaaata tggtcctggt gaattaatta
     1621 cttcagattt caatggttat ttaattacta aaaatgatga agatgcccta tttgataaag
     1681 taaaatatgt gattgatcat cctgaagtac aacaacgact gtcaaaaggt agtttggcaa
     1741 aagcgcaaca atatagtaaa gcgtcgttaa ttaaacaatg ggaccaattt gttcgtctaa
     1801 tataaatgta ctttaccttt atttttataa aaaaaaagca ttttcctatg tcatttaatc
     1861 aaacttgtca gttcttatga acgtggattg ttaaatagat gattttagga aaatgctttt
     1921 ttcttttgac ttagtttaaa attttttgcc actttgtact gatagtagtt gcattgtact
     1981 gttgtgcaga ttctatgcta ttaattgaaa attgttgcat catttggcta ttatttaata
     2041 attggtcaac cttttcaacc atttgattga tgtcacctgg aggtactaaa tagccattaa
     2101 atccatcttg aatcagttct gatggcccat aatctacatc ataactgatc actggtgtac
     2161 ctactgaaag cgactctaaa attgctaagc caaaaccttc cattttactt gtcgataaca
     2221 tcagttctgc tttagcaatc tcttcattaa tatgcgtctt aaaaccatga aatttaacat
     2281 gttccgataa atgataacct tctacaagtt gtcgatattc tgacaaacca tttccatgtc
     2341 cataaatatt caattgaatt ttgggatgtt tggttactaa ttgcttgatc acttcaattt
     2401 gatgtgtaat ttgtttgttt tcaacgaggc gagcaattga tatgatatga tttttatcct
     2461 tttgattgat gtcaaattga tactttaagt ttgccacgta gccaaccgga atattgataa
     2521 ctggtatttt attttcaata tattgtgaaa tatcttggca ttgcttttct gttgatacaa
     2581 caatcgcttt ataacgtgtt aaattattaa acactgtttt ataaaaattt tttatatcat
     2641 taccggtgcc ggataaatgt gtactgtgga gcacaacaat aactggaata ctttgattta
     2701 atcccgcaat aacatttcct aattcatgag gacggtctaa tatgatttga tcattatttt
     2761 tacataattg atggagaaaa tattgaacta attcatcttc tgtatcaaaa aattgttgat
     2821 gctggtcttc atttaagata acctttgtga gcgtgttttt cccttttata tcgtcgaaat
     2881 atttttggat gacgatttcc ccattaggtg tatagtaatt ttcgagtaca atccgttgtc
     2941 cttcacctaa aattcgagaa caacttaaaa agcctcttcc atcatacaat tcacgtttta
     3001 cttttcttct tttatgatca aaataattca catagtttaa ttgatgatac tgtttatcta
     3061 aaaaatgagc atacattata aattgctctt catcatatat tctgacatca tttgaatttt
     3121 ccacaaattt caacgtgtac ctacatgact tttcccaata ctgtatccag ttaacttgct
     3181 ttgtcttttt ataattgatt gctttttgaa aatagtcata cattgtaaac agatcatttt
     3241 caatctgatg ttgcttcgca tatgtgtatg aataaggatt ccatttaaca tatacacatt
     3301 ttgaagatat gccgtgttgt ttgaacaact tcagtctatt tatttgtgct ttttctacac
     3361 ctgtaatttt actttctaaa attgttccta aaatgtaatt catattatcg cctcatataa
     3421 gttttattcc gtatctttat tgtttatttt atatgaaaaa tacatctatt gcatgtgtaa
     3481 tcataaaaaa accaggccac aaggacctgg gtcaaattgt attatttgtt ttgttttttg
     3541 cgacgaccga ataacaataa tgaacctaat gctgcgaata atccaccaaa taacgttgcg
     3601 ttatttgagc cgttattttc actacctgtt tctggtaatg ctttggcttt attgtgatgg
     3661 tctttagtag cactcattgg tttcacaggt gtatgttttc ctgcatctga atctgagtcg
     3721 ctatccgaat ctgagtcgct atctgaatct gaatcgctat ctgagtctga gtcactgtct
     3781 gaatctgagt cgctatctga
//
LOCUS       DAMZPP010000191         3794 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMZPP010000191 DAMZPP010000000
VERSION     DAMZPP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.192"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(33..353)
                     /locus_tag="POH73_002627"
     CDS             complement(33..353)
                     /locus_tag="POH73_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDI6888535.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDENFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            412..950
                     /locus_tag="POH73_002628"
                     /pseudo
     CDS             412..950
                     /locus_tag="POH73_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(974..1234)
                     /locus_tag="POH73_002629"
     CDS             complement(974..1234)
                     /locus_tag="POH73_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6888536.1"
                     /translation="MEQITLTKEECVEQCINKDLKLLDYRVQQILEGVLSESTTYGDA
                     RNKLETLKIIAESHFKTEHASVIYKLALKKLDEKINATPIKE"
     gene            complement(1247..1489)
                     /locus_tag="POH73_002630"
     CDS             complement(1247..1489)
                     /locus_tag="POH73_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HDI6888537.1"
                     /translation="MIYNFDYSLLYERMAEYRYSQSSLANAIPISRTSINHKLQGKNL
                     FTQWEIKRICELLEIPPTKVGRYFFEQNVQKPVQMS"
     gene            1653..2369
                     /locus_tag="POH73_002631"
     CDS             1653..2369
                     /locus_tag="POH73_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6888538.1"
                     /translation="MNSFKDRLKQIMSERKISQSELSRRTGIGRNSISDYLNGKYEAK
                     QDKVFELAKALNVNEAWLMGFDISKNRKIENNDITSIYSKLTPPRQSNVLKYATNQLE
                     EQNNDSDNLVDFNSYIQEKSEVDIYGYASAGIGERLYNEPISKEFVRGYVPAHDIALK
                     VNGDSMEPLFKNGQIIFIEKSHTIKDGQIGVFIINGDAYVKKVYVEDNRLTLVSLNKK
                     YKDLYFYDNESVRLVGKVIL"
     gene            2381..3235
                     /locus_tag="POH73_002632"
     CDS             2381..3235
                     /locus_tag="POH73_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDI6888539.1"
                     /translation="MKPRKQDEKILSDQYSYFEPIISDSCDIKFDENKRRMGSIFISH
                     EEICFIRKEEDYIFKISLSEVIDYNTVVTIWKNQAFLTLNDNRKLTVYFVTNSPLTGF
                     ISILKTYMQLSKNKETIISNDCLPINDDEQTKVEIFDVVGLNYEGRRKELKKLIKKMK
                     NNDDFFFLYSDLKGNELKEELLYEDKVYEISDYEVIPGVFLQKEPDNPYDENAIKVMI
                     SNEYSEFHVGYVPREYASRLVNHMDNIVSCNAYINGGKYKTLDYLEEKIVTKESDYGL
                     RVHLEYKV"
     gene            3309..3455
                     /locus_tag="POH73_002633"
     CDS             3309..3455
                     /locus_tag="POH73_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888540.1"
                     /translation="MDFKEVDINIEEWEMVEIPFYTEEELTYRLNNGLPITKSELEEQ
                     ESKK"
     gene            3452..3637
                     /locus_tag="POH73_002634"
     CDS             3452..3637
                     /locus_tag="POH73_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888541.1"
                     /translation="MSSYKEIEHLHINTGGKELTQEQIEEAKAFIDSQEFKDMIREAK
                     ESRQRVMESKITDRTKM"
BASE COUNT         1368 a          539 c          625 g         1262 t
ORIGIN      
        1 tactgctaat aggtagcttt tataaatgtt gctcatttta ttctctcctt atatatttca
       61 ttgaaatgct catcgacgaa tttattcatc tttcttgcgt taaatctcca gcgattaaaa
      121 ttctcatcag ggtaatgcac aattccttgc gctcttagtt ctttttcaaa tctaggatga
      181 aatagtaatc tgtccttgat agtctcatca gatgcaattt ttaatttctt ctttaagtca
      241 ctcatgttcc atacagggtc taatgaataa ccaattagct catcatattc atcttttgtg
      301 ataagtacat gtgtttcagg tattggaact gttacgttta aaatatgtgg catttctatc
      361 tttcctttcg tgtataatgt tgttatctcc tagtgaaagg aggtgataag tatggacata
      421 atcgcgattt gtatcgcaat ttttagtttc ttactgactg cacttaaata ttatttagac
      481 tatatgaaag attctcttaa catcgatgtt atacctacca gaagctttaa ttacttggtc
      541 gatgacaaat caagttacaa cgatataaca tttattaatt tcacaaagtt tcccatttct
      601 gttattgaag ttgaatttga tattaaaaat aaagtaaatg aacaaaaaac gttcaaacct
      661 atacgatata aagataaaaa ctactccatt ccatttactt taggacctta tgaaagtgta
      721 gaatgtactt tttgctcgaa gaatatccag tgatatggga atgggatgtg actatcaaag
      781 tcactaccaa caaaggaatc tatataaagc ctgttatcat agaatcgcgg acagaacacc
      841 gagaatcaga gccacaagtg acagagttaa catcagcaaa taaggtaagt gctctttcca
      901 accccaagga tggtttttta aagaagtttt tatatcattt aaaaccttaa acatttaaaa
      961 tcctcccttt ccgtcactct ttaattggag tggcgttgat tttttcgtct aactttttca
     1021 atgctaattt gtaaataact gaagcatgtt cggttttaaa atgagattca gcaataattt
     1081 tcaatgtttc taatttattt cttgcatcac cgtatgtggt actttctgat agaacacctt
     1141 ctaaaatttg ttgaactcga taatctaaaa gttttaagtc tttattgatg cattgttcga
     1201 cacactcttc tttggttaac gtgatttgtt ccatagtgtc ctcctgttac gacatttgta
     1261 caggtttctg tacattttgt tcaaaaaaat atctacctac ttttgttggt gggatttcta
     1321 ataattcaca gattcgtttt atttcccatt gtgtaaataa attttttcct tgcaacttgt
     1381 gattaataga tgtccttgaa atagggattg cgttcgctaa agaactttgg ctatatctat
     1441 actctgccat tctttcgtac agcaaactat aatcgaaatt gtatatcata aactcacctc
     1501 ccttcttgtt cggttttctg tacaaatcaa ttaaaacacc tttgtttaaa taagtcaaca
     1561 cataaaatac atttttctgt acaatatttg ttaaaaatta ttgataatcg tcattgtacg
     1621 tagtattatg ttcttaggag gtgttcagaa atatgaacag ttttaaggat agattaaagc
     1681 aaattatgtc tgaacggaag atatctcaat cagagctatc aagaaggact ggtattggta
     1741 gaaactcaat tagcgattat ttaaacggaa aatatgaagc gaaacaagac aaagtctttg
     1801 aactagcaaa ggctttaaac gttaacgaag cgtggcttat ggggtttgat atttctaaga
     1861 atagaaaaat tgaaaataac gacatcactt ccatatacag taaactcacg cctccaagac
     1921 aaagcaatgt actaaaatat gcgactaatc aattagaaga acaaaataat gacagtgata
     1981 atctggtaga tttcaattct tacattcaag aaaaatccga agtggatata tatggttatg
     2041 cgtcagctgg tattggcgaa agattatata acgagcctat ttcaaaagaa ttcgtaagag
     2101 gttatgtccc cgcacatgat atagctttaa aagtaaatgg agactcaatg gagccgttat
     2161 ttaaaaacgg acaaattata ttcattgaaa aatctcacac tatcaaagat ggacaaatag
     2221 gcgtctttat tataaatgga gatgcttacg taaagaaagt ttatgtagaa gataatagat
     2281 taacgttggt ttctttaaat aaaaagtata aagatttata tttttatgat aacgaaagtg
     2341 tgaggttagt tggaaaagtt attttatagg aggtagtaaa atgaaaccta gaaagcaaga
     2401 tgaaaaaata ttatcagatc aatacagtta ctttgaacca ataatcagcg acagttgcga
     2461 cataaaattc gacgaaaaca agaggagaat gggttctata ttcatttcac atgaagagat
     2521 ttgttttata aggaaagaag aagattatat attcaaaatc tcattatcag aggtgataga
     2581 ttataacact gttgttacta tttggaaaaa ccaagctttt ttaacattaa acgataatag
     2641 aaaattaaca gtttatttcg taacaaactc tcctttaaca ggattcatct caattttaaa
     2701 aacttatatg caattatcta agaataagga aacaattatc tcgaatgatt gtctacctat
     2761 taatgatgat gaacaaacta aagttgaaat tttcgacgtc gtaggattaa attatgaagg
     2821 tcgtagaaaa gaattaaaga aacttatcaa gaaaatgaaa aataacgacg atttcttttt
     2881 cttatatagt gatttgaaag gaaatgaact taaagaagaa ttactttatg aagacaaggt
     2941 gtatgaaatt tctgattacg aggttattcc tggtgtattc ttacaaaaag aaccggataa
     3001 tccttatgat gaaaacgcga taaaagttat gatttcaaat gaatactctg aatttcacgt
     3061 tggatatgta cctagagagt atgcttcaag attagtcaat catatggaca acatcgtttc
     3121 ttgtaacgca tatattaatg gtggtaagta taaaacttta gattatttag aagagaaaat
     3181 cgttactaaa gaatcagact atggattacg agtacattta gaatacaaag tttgagatag
     3241 gtaaagattg tatttttata agtaattact ataaataata gaaaattcat ttcacaggag
     3301 ggtttaacat ggattttaaa gaagttgaca ttaacattga agagtgggaa atggttgaaa
     3361 tcccctttta tacagaagaa gaactgactt ataggttgaa taatggttta cctataacta
     3421 aaagtgaact tgaagaacag gagtcgaaaa aatgagttct tataaagaaa ttgaacactt
     3481 acacatcaat actggtggta aagagcttac tcaagaacaa atagaagaag ccaaagcttt
     3541 tatagacagt caagaattta aagatatgat tcgagaagct aaagagtcac gtcaaagagt
     3601 tatggagtct aaaattaccg atagaactaa aatgtgatta atagcgccta tgtggcgctt
     3661 taatataaat ataaacaaag gagaaattga catgaaaaaa gcaatcttaa ctttaagtct
     3721 tatatttatt acctactacc tcacttttaa atatatgtgg attaaagaat tgaagtatta
     3781 attatgctta tttg
//
LOCUS       DAMZPP010000192         3759 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMZPP010000192 DAMZPP010000000
VERSION     DAMZPP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.193"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..222)
                     /locus_tag="POH73_002635"
     CDS             complement(<1..222)
                     /locus_tag="POH73_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6888542.1"
                     /translation="MSIVQLYDITQIKSFIKHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFK"
     gene            288..383
                     /locus_tag="POH73_002636"
     CDS             288..383
                     /locus_tag="POH73_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888543.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            409..2544
                     /locus_tag="POH73_002637"
     CDS             409..2544
                     /locus_tag="POH73_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDI6888544.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPLRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYYTLSL
                     TVKGFDFQLESNQRYTNKETLEQIVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMSPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            2657..>3759
                     /locus_tag="POH73_002638"
     CDS             2657..>3759
                     /locus_tag="POH73_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDI6888545.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGV"
BASE COUNT         1357 a          547 c          694 g         1161 t
ORIGIN      
        1 tttgaattta tttttatcgt atgcaaaaga caatatgatt gctttaattg aatcgttttc
       61 tttaattgaa aatacaccag gtgattcaat cgcatcggtt attaatacat ctatttcatt
      121 gtactgttga ggaagtttat ataagtatga tgctgattca taattcgaat gtttaatgaa
      181 cgattttatt tgtgtaatat catataactg aactatgctc atgtcattac ctccgtactt
      241 tttgttactt ttattatata gtatttcaac tgaaatgaaa gttaatagtg atattaacat
      301 gttacaatac atttaacaca tcatttgcaa tcattgaatt taaatcaaag attagtggaa
      361 tagatgaagc acgttcgaaa taaaagaaag tatgagaaag gataatttat gaaatcttta
      421 atattagctg aaaaaccatc agtcgcaaga gatattgctg atgctttaca aataaatcaa
      481 aagcgtaatg gttactttga aaataaccaa tatattgtca cgtgggcgtt aggtcatcta
      541 gtgacaaatg cgacacctga acaatacgat aaaaatttaa aggaatggcg attagaagac
      601 cttccaatta tacctaaata tatgaaaact gttgttattg gtaaaacaag caaacaattt
      661 aaaacagtaa aagcgttaat tttagataat aaagtgaaag atattattat tgcaacagat
      721 gctggacgag aaggtgaact agttgcaaga ttgattttgg ataaagttgg taacaaaaag
      781 ccactccgtc gattatggat tagctcagtt actaaaaaag ctattcaaca aggttttaaa
      841 aatttaaaag acggtcgcca atataacgat ttgtattatg cagcgttagc gagaagtgaa
      901 gcggattgga ttgttgggat taatgcaacg cgtgcactaa caacaaagta tgatgcacag
      961 ctatccctgg gacgtgttca gacaccaacg attcaattag taaatacacg acaacaagag
     1021 attaatcagt tcaaaccaca acaatactat acattatcat taacggtaaa agggtttgat
     1081 tttcagctag aatcaaatca gcgatatacc aataaagaaa ctttagaaca gatagttaat
     1141 aatttgaaaa atgtcgatgg taagattaaa tctgttgcta ctaaacataa gaagtcgtat
     1201 ccgcaatcac tgtacaattt aacagattta caacaagata tgtatagacg ttataaaatt
     1261 ggacctaaag aaacattgaa tacacttcaa agtttatatg agagacataa agtcgtaacc
     1321 tatccaagaa cagattcaaa ctatttaaca actgatatgg tagatactat gaaagaacgt
     1381 attcaggcga cgatggcaac aacatataaa gaccaagcgc gcccattaat gtctaaaaca
     1441 ttttcatcaa aaatgtcgat atttaataat caaaaagtat ctgatcacca tgcaattatt
     1501 cctacagaag tgagacctgt catgtcagac ttaagtaata gagaattaaa gttatacgat
     1561 atgattgtcg agcgtttttt agaagcttta atgtctccgc acgagtatga cgcgataact
     1621 gttactttag aggttgcagg gcacacattt gttttgaaag agaatgtaac aactgtatta
     1681 ggctttaaat ctattagaca aggtgaatct attacagaga tgcaacagcc tttttcagaa
     1741 ggcgatgaag tgaagatttc aaaaacaaac attagagaac atgaaacaac acctccagaa
     1801 tattttaatg aaggttcgtt attaaaagcg atggagaacc ctcagaactt tattcaattg
     1861 aaggataaaa aatatgcgca aacattaaaa caaacaggtg gtatcggcac agttgcaaca
     1921 agggccgaca ttatcgataa attatttaat atgaatgcca ttgaatcaag agacggtaaa
     1981 attaaagtga cgtcaaaagg taaacaaata ttagaattag caccagaaga attaacgtcg
     2041 ccacttttaa ctgcacaatg ggaagaaaaa ttacttttaa ttgaacgtgg taaatatcag
     2101 gcgaaaacat tcattaatga aatgaaagat tttacgaaag atgttgtaaa tgggataaaa
     2161 aatagtgatc gtaaatataa acacgataat ttaacaacca ctgaatgccc aacgtgtggt
     2221 aaattcatga ttaaagttaa aactaaaaat ggtcagatgc ttgtgtgcca agatccatct
     2281 tgtaagacga aaaagaatgt acagcgcaaa acaaatgcaa gatgtccaaa ctgtaaaaag
     2341 aaattaacgt tgtttggtaa agggaaagaa gcggtatatc gttgtgtttg tggacattct
     2401 gaaacgcaag cacatatgga tcagcgtatg aagtctaaat cctctggtaa agtatctcgt
     2461 aaagaaatga aaaagtatat gaataaaaat gaaggtttag acaataatcc gtttaaagat
     2521 gcattaaaaa acttgaattt atagataaaa tcgaacaaag ttgaatcata aaatcgaaaa
     2581 gttcgctttt ggtattgttt tttattaaga atgatattaa actattaagg tattttaaaa
     2641 aaaggagcat ccattcgtga aaaactattt ccagttcgat aaatatggaa caaactttaa
     2701 aagagaaata ttaggcggta tcacaacttt cttatctatg gcctatattt tagcagttaa
     2761 cccgcaagtt ttaagtttag caggtgttaa aggcgtatca gaagatatga aaatggacca
     2821 aggtgccatt tttgtagcga ctgcattagc agcatttgta ggctcgctat tcatggggct
     2881 aatagctaaa tatccaattg cgttagcacc aggtatggga ttaaatgcat tctttgcatt
     2941 tactgttgtg ttaaccatgg gtattccttg gcaagttggt ttaacaggtg ttttattctc
     3001 aggtatattc tttgcgatat taactgtcac agggtttagg gaagttatta ttaatgcaat
     3061 tccttatcaa atgaagatgg ctgtttcagc aggtataggg ttattcatta catttgtagg
     3121 tttacaaagt tcgggtatta tagttaaaaa tgaatctaca ttagtaacat tgggacattt
     3181 aactgatcca ccagtacttt tagcgatatt cggtatcgtt attacagtta ttttatatgc
     3241 aattaaattg ccaggttcta tctttatcgg tatgatcatc acagcaatcg ttggtatgtt
     3301 cactggattg attcaaatgc catctggtat tgtaggtaag attccaagta ttgaaccaac
     3361 gttcggcgct gcatttgaag cgtttaaaga cccaagtcaa ttattaacga tacagttctt
     3421 aattgttatt ttaacgttct tatttattga tttctttgat acagctggta cattagtagc
     3481 cgttgctact caagcaggta ttatgaaaga taataaatta ccaagagctg gtagagcatt
     3541 gttctcagat tctttagcta cgattgtagg atcagttttt ggaacaacta caacaacttc
     3601 ttatatcgaa tcaacttcag gtgttgctgt aggtgctaga acaggatttg ctagtatcgt
     3661 aacaggttgt tgtttcctat tagcgctatt ctttagtccg ttaatcgctg ttgtaacaag
     3721 tgcagttacg acaccagctt tagtggtggt aggtgtatt
//
LOCUS       DAMZPP010000193         3623 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMZPP010000193 DAMZPP010000000
VERSION     DAMZPP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.194"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(205..1407)
                     /locus_tag="POH73_002639"
     CDS             complement(205..1407)
                     /locus_tag="POH73_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDI6888546.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            1725..2795
                     /locus_tag="POH73_002640"
     CDS             1725..2795
                     /locus_tag="POH73_002640"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDI6888547.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITTEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSIGISKCSNEVELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            2810..>3623
                     /locus_tag="POH73_002641"
     CDS             2810..>3623
                     /locus_tag="POH73_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDI6888548.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FEGENVDGHRFVSKALQDGAGAAFYQKGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCCVDDRDTTGISFTINN"
BASE COUNT         1352 a          535 c          630 g         1106 t
ORIGIN      
        1 atatcaaaaa agctaatttc tatcaaataa ttaatagaaa ttagcttttt tacattgcct
       61 aagaacttaa tgtctcaagc acttcaaaga gggattgtta ttatcgagta atatctatat
      121 atgacatttt aaatcgacga cgcaaataga ctatataacg tcatcttgaa aagttatgat
      181 atttgaaact caaaatagtt taaattaatt actttttgga tggtataaat ctacatatcg
      241 ttttggttca tgataataaa ttgataagac tatacctatt ccagtcatca tactccatag
      301 cgcactacca ccataactaa taaatggtaa tggaatacca gtgattggta acaactgaat
      361 tgtcatacca atattttgta aaatatggaa cacaagtaaa gtgacgaaac caacgataaa
      421 gattttgtta aattgatctt caattttcgc agctaatctt attagatgga agattaaaaa
      481 taaaaatatt aagatcaata tgacagaacc gataaaacca agttcctctc caatcactga
      541 aaagataaag tcagtatgat tttcaggtat ataaacctca ccgtgattgt atcctttacc
      601 tagtaactgt cctgaaccga tagctttaag tgattcagtt aaatgatagc catcaccact
      661 actatatgta taggggtcaa gccatgaatt gattcgtccc atttgataca gttggacacc
      721 taataaattt tcaattaatg cgggtgcata tagaatacct aaaatgactg tcattgcacc
      781 aacaatacct gtaataaaga taggtgctaa gatacgccat gttataccac ttactaacat
      841 cacacctgca ataattgctg ctaatactaa tgtagttcct aggtcatttt gcagtaatat
      901 taaaatactt ggtactaacg agacaccaat aattttgaaa aataataaca aatcactttg
      961 gaatgattta ttaaatgtga attgattatg tctagaaacg acacgcgcta atgctaaaat
     1021 taaaataatt ttcatgaatt cagatggctg aatactgata gggccaaacg tgtaccaact
     1081 tttggcacca ttgataatag gtgtaatagg tgactcagga ataacgagca atcctattaa
     1141 taatagacag attaagaaat acaataaata tgtataatgt ttaatctttt taggtgaaat
     1201 aaacatgatg atacctgcaa aaattgcacc taaaatgtaa taaaaaattt gtctgatacc
     1261 gaaattagca ctgtattgac caccgcccat tgccgagtta ataagcagaa cactgaaaat
     1321 tgctaaaaca gctatagtgg ctactaatac ccagtctact ttgcgaagcc aatgcttatc
     1381 cggctgttga cgagatgaat aattcattgc aaactccttt tatactcact aatgtttata
     1441 tcaattttac atgacttttt aaaaattagc tagaatatca cagtgatatc agctatagat
     1501 ttcaatttga attaggaata aaatagaagg gaatattgtt ctgattataa atgaatcaac
     1561 atagatacag acacataacg cctcgttttt aaaatgcaaa acagcattaa aatgtgatac
     1621 tattaagatt caaagatgcg aataaatcat ttaacaattg gactaaatca atattaattt
     1681 atattaaggt agcaaaccct gatatatcat tggaggaaaa cgaaatgaca aaagaaaata
     1741 tttgtatcgt ttttggaggg aaaagtgcag aacacgaagt atcgattctg acagcacaaa
     1801 atgtattaaa tgcaatagat aaagacaaat atcatgttga tatcatttat attaccaatg
     1861 atggtgattg gagaaagcaa aataatatta caactgaaat taaatctact gatgagcttc
     1921 atttagaaaa tggagaggcg cttgagattt cacagctact gaaagaaagt agttcaggac
     1981 aaccatacga tgcagtattc ccattattac atggtcctaa tggtgaagat ggcacgattc
     2041 aagggctttt tgaagtattg gatgtaccat atgtaggaaa tggtgtattg tcagctgcaa
     2101 gttctatgga caaacttgta atgaaacaat tatttgaaca tcgagggtta ccacagttac
     2161 cttatattag tttcttacgt tctgaatatg aaaaatatga acataacatt ttaaaattag
     2221 taaatgataa attaaattac ccagtctttg ttaaacctgc taacttaggg tcaagtatag
     2281 gtatcagtaa atgtagtaat gaagtggaac ttaaagaagg tattaaagaa gcattccaat
     2341 ttgaccgtaa gcttgttatt gaacaaggcg ttaacgcacg tgaaattgaa gtagcagttt
     2401 taggaaatga ctatcctgaa gcgacatggc caggtgaagt cgtaaaagat gtcgcgtttt
     2461 acgattacaa atcaaaatat aaagatggta aggttcaatt acaaattcca gctgacttag
     2521 acgaagatgt tcaattaacg cttagaaata tggcattaga ggcattcaaa gcgacagatt
     2581 gttctggttt agtccgtgcg gatttctttg taacagaaga caaccaaata tatattaatg
     2641 aaacaaatgc aatgcctgga tttacggctt tcagtatgta tccaaaatta tgggaaaata
     2701 tgggcttatc ttatccagaa ttgattacaa aacttatcga gcttgctaaa gaacgtcacc
     2761 aggataaaca gaaaaataaa tacaaaattg actaactgag gttgttatta tgattaatgt
     2821 tacattaaag caaattcaat catggattcc ttgtgaaatt gaagatcaat ttttaaatca
     2881 agagataaat ggagtcacaa ttgattcacg agcaatttct aaaaatatgt tatttatacc
     2941 atttgaaggt gaaaatgttg acggtcatcg ctttgtctct aaggcattac aagatggtgc
     3001 tggggctgct ttttatcaaa aaggcacacc tatagatgaa aatgtaagcg ggcctattat
     3061 atgggttgaa gacacattaa cggcattaca acaattggca caagcttact tgagacatgt
     3121 aaaccctaaa gtaattgccg tcacagggtc taatggtaaa acaacgacta aagatatgat
     3181 tgaaagtgta ttgcataccg aatttaaagt taagaaaacg caaggtaatt acaataatga
     3241 aattggttta cctttaacta ttttggaatt agataatgat actgaaatat caatattgga
     3301 gatggggatg tcaggtttcc atgaaattga atttctgtca aacctcgctc aaccagatat
     3361 tgcagtcata actaatattg gtgagtcaca tatgcaagat ttaggttcgc gtgaggggat
     3421 tgctaaagct aaatctgaaa ttacaatagg tctaaaagat aatggtacgt ttatatatga
     3481 tggcgatgaa ccattattaa aaccgcatgt taaagaagtt gaaaatgcaa aatgtattag
     3541 tattggtgtt gctactgata atgcattagt ttgttgtgtt gatgatagag atactacagg
     3601 tatttcattt acgattaata ata
//
LOCUS       DAMZPP010000194         3613 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMZPP010000194 DAMZPP010000000
VERSION     DAMZPP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.195"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..239)
                     /locus_tag="POH73_002642"
     CDS             complement(<1..239)
                     /locus_tag="POH73_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HDI6888549.1"
                     /translation="MGVAYVFSKIQPKATMIATITLVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSA"
     gene            complement(532..1499)
                     /locus_tag="POH73_002643"
                     /pseudo
     CDS             complement(532..1499)
                     /locus_tag="POH73_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     regulatory      complement(1558..1656)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1645..1914)
                     /locus_tag="POH73_002644"
     CDS             complement(1645..1914)
                     /locus_tag="POH73_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDI6888550.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIFLL"
     gene            complement(1911..2606)
                     /locus_tag="POH73_002645"
     CDS             complement(1911..2606)
                     /locus_tag="POH73_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDI6888551.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIIL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWVIPCVAGALFGEYISNPQ
                     ALGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMER"
     gene            complement(3250..>3613)
                     /locus_tag="POH73_002646"
     CDS             complement(3250..>3613)
                     /locus_tag="POH73_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDI6888552.1"
                     /translation="ILEELGLPYRRVILCTGDIGFSASKTYDLEVWLPSYNDYKEISS
                     CSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRTFAAIVENYQNEDGTVTIP
                     EALVPFMGGKTQISKPVK"
BASE COUNT         1182 a          686 c          540 g         1205 t
ORIGIN      
        1 gcacttaaaa cgaaagtatt acctaaaaca gttgttataa ttactgtaat aagtgcacta
       61 atcccgaaag attgtattga tttattccat aaaacgatac ctggtatggt tgcaaataat
      121 gcaaatatta gtcctaaacc aggcactaga tataaagcta aagcgacaaa taccaacgta
      181 atcgttgcaa tcattgttgc tttaggttgt atttttgaaa acacataagc cactcccata
      241 tttttaacta tagctattat tttaacctct ttaatggaaa ttaacaattt atagattgta
      301 tgcttctatt tcatttaatt gaataataac tttcatgttt tataagtaat taacatactc
      361 atttaaatcg cttttgtgtg ctttcatttt caacatgatt atttaatccc actacatagc
      421 aatcaagctt gatttagatt tacaattcat ttccactctc atgtactcta gatgtttttg
      481 aatatgataa ctgtgattta gtggcttcat tctttgaaaa tatatacatt attacttgcg
      541 cttaaaatgc tttaaattta agaaatgata taagttaggt gcccaggtac taaagtttag
      601 taggaatcca tcatgcccaa cgttatcagg tacaaagaaa tgacgatgat atttaaaacg
      661 ttcacctaaa gcacgaactt gatcatccgg atatagcaaa tcatctataa agcccattgt
      721 taagactttc gtttctaaat ttttaaaaac atgcgttacg tctgtgcgac ctcggtcaat
      781 gttgtgacta tccaatacat ctagcagtgt cagataacaa ttcaaatcga atcgttcttt
      841 aaatttatta ccttgatgtt gctggtatgc gactacttca tccggtgtga aacgttcatc
      901 ataacttttc gatgatcgat atgtcaaaaa acctaattgg cgtgcaatac ttagaccttc
      961 cttaccacca agatgaatgc ctgacgcgca atttcattga aagctctact ataagatgat
     1021 gttcgacttg ttgcagcaag aataatggct ttatctactt caaactgtcg attgtaaagt
     1081 agttccattg cttgcatacc tccaagactt ccccctatta aaatattaat tttgtcataa
     1141 ccaagcgctt gtatacctcg ttcattcgct ctaacaatat ctcttaatgt taatttttta
     1201 ggaaaatgag ggtcgtttaa aggtgaactt gaaccgaaag gactaccaat aacatcaaat
     1261 gttaaaaatt gatagtcatg aataggcaca tatcccccat caataatttc tcgccaccac
     1321 cccggataat catctgttcc atatgttaaa tgattgccag ttaatgcatg acaaactaca
     1381 actaatggtt gtccatgata accgacatgc tcatatctca aacgcaagtt atctatgact
     1441 tccccagatt ctgttataaa ttcccctaaa tttaaagtat ctactgtgta atttgtcatt
     1501 gttctttcct ccttaaacaa aaaacttctc accctattga aaagtaagaa gtctttatac
     1561 ttatcattcg agtaactcgt tggttttagc accgtgctat aaagtcggtt gctgaagtat
     1621 cacagggcca agtccctcaa cttctcacaa taagaaaata gataaaataa ccgtcggaat
     1681 gagcgcgata atgtaaggga tgtttaatgt atacccctca ccatgaggcg tctgttgaat
     1741 aatgctgtca atgacaagtg ccgtaaatag tgtgattggg ataaatgata gccatcgaac
     1801 cacgacatca ggcaattgca cttttgaaat catgataaaa ggtataattc gaattaatag
     1861 cgttacgatt ccacacaata aaataagtat taacatgttc atatgagtta tcatcgttcc
     1921 atcatcactc ctaacgctgc tgaaattgtg gctgcaatta atattgctag atatgaaggc
     1981 ataaacatac ttagcgataa catcattact atgacggcaa taatgagtac tatgtaaatt
     2041 cttaaacgcg atttagtaat tgattcaaat tgtgcaatgg ctaaaaagat aaacatagcc
     2101 gtgatagcaa aatctaatcc taatgcttgt ggattcgaaa tatactcgcc aaacaatgca
     2161 cctgctacac atggaattac ccaaaataaa tatgctgtaa tattaagacc atgcatccaa
     2221 cgatcattga tagcttctcc ttttaaataa ggtgtaatgg cgacaccaaa cgtttcgtca
     2281 gttactaatg aacctaatcc aacacggttc caaaacccat atgtcttgaa gtttggtgca
     2341 agcgacatac ttaaaaggaa cattcttgaa tttacgataa atacagttag tacaatcgct
     2401 gatatcggtg tacctgctat aaacaacgcg cacataataa attgcgcagc accggcatat
     2461 ataacaagac ataacaagat aatttctaaa atactaaagt tttgagatga agccacgata
     2521 ccaaatgaaa taccaacacc ggcataaccc aataatgttg ggatacactc ttgcacgcct
     2581 tgtctaaaac tcaaatgtgt tgtcatctca attacctcct ttttcacttt atatttatat
     2641 aattacgaaa aaataatata cctagtctaa tatagttcta tatgacaagc aataacgtta
     2701 gactccggtt tgtctgaggt tagacatacc ggagtcttta gtcggcttca tatcagcttt
     2761 ttagttttga gaattttcaa tattttattt aatactacct ccatattttc tacgggtttg
     2821 tagttgtttt taagtactaa ttttataatt ttttatatct aatgatgagt ctgggatatt
     2881 gattcatgtc tctaacactt tcgataatcc cccctaccct actgcatcga tattactcat
     2941 actacatcaa cgaaatcagt tttttatcac ttaatttccc ataatagtga tgctcaaaat
     3001 tgttacgttt tagattgttt tagttcatca taattatccc gtattgttgc tataatgaaa
     3061 tgcgttcacc ccattaaaca acaaacttaa tttattgttg ttatgtgcat tggctcacta
     3121 ttatactttt acagcacaaa aaaagtggcg acagcttcgt caccactttt taaaatatta
     3181 tttaaagtat cttggccttg ctttaagtat acgtagatat atacttttta aagcttgtag
     3241 ctaaagtctt tatttaactg gttttgaaat ttgtgtttta ccacccataa atggtactaa
     3301 tgcttctgga attgttactg ttccatcttc attttggtaa ttttcaacaa tagcagcaaa
     3361 tgtacgtcca actgctaaac cactaccatt taatgtatgt gctaattctg gtttagctgc
     3421 tttgtcacgc ttgaagcgga tgttagcacg acgcgcttgg aaatccgtac agtttgagca
     3481 tgaactaatt tctttataat cattgtagct tggtaaccaa acttctaaat catatgtttt
     3541 gcttgcacta aatccaatat cacctgtaca taaaataaca cgacggtatg gtaaacctaa
     3601 ctcttctaaa att
//
LOCUS       DAMZPP010000195         3488 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMZPP010000195 DAMZPP010000000
VERSION     DAMZPP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.196"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(65..262)
                     /locus_tag="POH73_002647"
     CDS             complement(65..262)
                     /locus_tag="POH73_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888553.1"
                     /translation="MLIKDVYRKNVIVTLKNDEKLKGFVIDFENPLESDSGNYCMDLE
                     TDLGFYSIDESDIKDIQIITE"
     gene            complement(264..917)
                     /locus_tag="POH73_002648"
                     /pseudo
     CDS             complement(264..917)
                     /locus_tag="POH73_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190211.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            complement(990..1241)
                     /locus_tag="POH73_002649"
     CDS             complement(990..1241)
                     /locus_tag="POH73_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000585092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888554.1"
                     /translation="MIIMRKLKEDNQVIVYEYIPQDKIEKGKGEITVNKLDSKVIDYK
                     LSKVENEKGILIYRDKSFHAILNFIDENKFPNEYIYAWY"
     gene            1746..1850
                     /locus_tag="POH73_002650"
                     /pseudo
     CDS             1746..1850
                     /locus_tag="POH73_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1849..2331
                     /locus_tag="POH73_002651"
                     /pseudo
     CDS             1849..2331
                     /locus_tag="POH73_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019168928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2762..3178)
                     /locus_tag="POH73_002652"
     CDS             complement(2762..3178)
                     /locus_tag="POH73_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888555.1"
                     /translation="MFKVNYSILSYYPDIYLHSNLAVGVAFEIIGESYHKNEIKFITQ
                     RRKILSFDDEIDNLELINMFLDGLKCEFEQTNQSLKSYKKKFVNNFYFENIEYKMFDS
                     LKEVNNFIENTYKYVLHHGLDKKERLKKTEKELLNS"
BASE COUNT         1223 a          523 c          399 g         1343 t
ORIGIN      
        1 attggaaggt taaaaaaatg tataaaaata gcaccacttt ctctttatct gtgtagaaag
       61 gatgctattc tgtaattatt tgtatgtctt taatatctga ttcatcaata gaataaaaac
      121 ctaaatctgt ttctaaatcc atgcaatagt taccagaatc actttctaac ggattttcaa
      181 aatctataac aaaacctttt aatttttcat cattttttag tgtaactata acatttttac
      241 gatatacatc tttgattaac attttacttc tccttactta caaatggtac tatatgagaa
      301 ccagttttag aataatgtat tttaccatat gcagtattaa tatattttcc acctgcataa
      361 gcttttccta tagttttttc gaaattaatt atttccttac gattaaatcc aaatttatcc
      421 actaatattt tgccagtcat tgagtatttc tttaataaat catttaattc ttctatagag
      481 agtattgtat agctaggaaa cctctcatca ttctgcaatg cataaatttt attttttaaa
      541 tataatttat gacctaaaga atgtctattc tgtttttcag gatttatttc tatttttatt
      601 ttaccacttt caatcatttc tttcatctgg tttttagctt tttcctgtaa ttttgcttct
      661 tttacttcta cttgatattt accttcacgc tctttaaaga acttgtctcg ccaattgcca
      721 acatgtggca ctgtggtact tctacaccaa ggatgcatag gtggcgcatt cacacctggt
      781 atcatatctt taactttaaa tatttttccg ttgagtgaat gacataattt agatgtttca
      841 ctatctattt tggcaacata tttatattcg ccatcttcac caagttcttt taaatatgtt
      901 aacttttgtg attctgctat ttcagtaatt agttaaaaaa acacataaaa atagcaccac
      961 tttctcttta tctgtgtaga aaggatgctt taataccatg catatatata ttcattcgga
     1021 aatttatttt catcaataaa atttaatatt gcatgaaatg atttatctct ataaattaaa
     1081 ataccttttt cattttcaac ttttgataat ttatagtcaa ttacctttga atctaattta
     1141 tttactgtta tttcgccttt ccctttttct attttatctt gtggaatata ttcatatact
     1201 ataacttgat tatcttcttt taacttcctc ataataatca atcaaatcac cctatttctc
     1261 aatataatta gaaaaattgt attttctttc tacaatccca tgtgcttctc tatagtaata
     1321 attatattta ttctcaaaca aatattcata agctctttta actaggtttt aattcaaatt
     1381 ggtattttta gaaattatat ctacttctat atccttattc ctttgacgta ctgatttata
     1441 atacattttt ttctttttta aattatataa tcatgtctta actcaatctt cgattggaag
     1501 ttatttaatt gagacatgac atttatgaag gaatgctatt ttacattatg aaaggtatta
     1561 aaccaaatta tgctgagctt ggtcgacaat ataattgtga ttcaagaaca gttaaaaaat
     1621 attatgaagc aggaaaataa aatgaattag aacgattgaa gaaaagacaa caaaataaga
     1681 aagcatcaaa gctagattca tttaaagaaa ttaataataa aaaaattgaa ttaggatata
     1741 cagctatggc catttttaaa tatatggaga aaaagggtta tgaaggtaaa tataatattc
     1801 tagaagaata ctgtaaaaat aaaatacaaa atgagactaa aaaaataaca tttttttgaa
     1861 gatagtataa atatttgttt cttagataac agtgttgttg gtaaaacaca cctaacaatc
     1921 tcattaggaa ttgaggcatg taaacaaaat ataaagacta gattctatac tttcaaagaa
     1981 ttaatagact tattaactgt cacagattcc aaagaaatca ttaataaaac attaaaacaa
     2041 ttaagcagaa tagagttact tatcattgat gaaatttgtt attctgccat caccaaagaa
     2101 caagctgatt tattctacca gttaatgtca ctaaggtatg aatggaaatc aacaataatt
     2161 acgactaata ttccattttc gagttgggga gagtcattta gtaataagat tgtatcagca
     2221 gctataattg atcggttagt tcaccattca aaagtattta aaattactgg agaatcatat
     2281 cgattaaaag attataaaac cgaaaaatca ttaaatatac gacaatctta aaccactaaa
     2341 aaacgacatt ttcaaacctc cataaaacag catttttaaa ccgctattga cattaagatt
     2401 atataaaagg caaatattaa agctaaaatt ccgattagat aattaataaa gattattatt
     2461 ctagttttag ttaagtttgt cattttcatc acctcgtaat ataatacgtg ggagggaata
     2521 tccccctcta taaaaaacat tttattattg tggcaagtgt tgcaaagcct aaagtgtagt
     2581 tgcggaagat ttcgcttttg gcttttgcga tatttgtcat tttttcttct ctttccaacc
     2641 tgttttgtat ccaacacttt tttaaaaact tttttcgttt ttttacataa aaaataaccg
     2701 catcaataaa ggtacggtta ttacttattt atattcagaa caatatgttt gaccttttta
     2761 cttagctatt caaaagttct ttctcagttt tcttaagtct ttctttttta tctaaaccat
     2821 gatgtaatac atatttatat gtattttcaa taaaattatt aacttctttt aatgagtcaa
     2881 acattttata ttcaatattc tcaaagtaga aattattaac aaactttttc ttataacttt
     2941 ttaatgattg attagtttgt tcgaattcac atttaagccc atccaaaaac atattaataa
     3001 gttctaaatt gtctatttca tcatcaaatg ataaaatttt tcttctttgg gttataaatt
     3061 taatttcatt cttatgataa ctttctccta taatttcaaa tgccacacct acagctaaat
     3121 tactatggag ataaatatct ggataataac ttaaaatcga ataattcact ttgaacataa
     3181 ctaatcactc cttgtacagt ggtgaagcaa gtattattat taaattgtag gagggaaagt
     3241 ccctcctgac tgattaacgc tcgaatgtcc attcaatctt tataaagtag agatttatta
     3301 agattaacaa tttggtacat ttgagctttt ttattccctt cactcgcact tacttaaact
     3361 tatatgctca ttatataaat ataaaaagct ataaaaattt aatggaaatg cccccttcga
     3421 agtagacatt gaaaaaatga aaactttgaa gggagcattt caagacgatc cattccaatc
     3481 ttttttat
//
LOCUS       DAMZPP010000196         3474 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMZPP010000196 DAMZPP010000000
VERSION     DAMZPP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.197"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..1529)
                     /gene="treC"
                     /locus_tag="POH73_002653"
     CDS             complement(<1..1529)
                     /gene="treC"
                     /locus_tag="POH73_002653"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDI6888556.1"
                     /translation="MLKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFERLINVAHEKGIKVMLDIVI
                     NHTSTEHEWFKAARKSKDNPYRDYYFFKASEDGPPTNWHSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTVGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKDSIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AV"
     gene            complement(1593..3020)
                     /gene="treP"
                     /locus_tag="POH73_002654"
     CDS             complement(1593..3020)
                     /gene="treP"
                     /locus_tag="POH73_002654"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDI6888557.1"
                     /translation="MAVKREDVKAIVTAIGGKDNLEAATHCVTRLRLVLKDESKVDKE
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKKAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKELIVMYPQIADISNMIN
                     VIANTAFIFLPALIGWSSMRVFGGSPILGLVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLVVTGIA
                     IVVPCILTIVMSNFSKQKAKEIVED"
BASE COUNT         1155 a          758 c          452 g         1109 t
ORIGIN      
        1 acagccgatg ctgagaaatt cgcaattact agccattgtt gattcttata atgacgttca
       61 taaacaaata aatgatcatg atccataaaa cgtggcacaa tgtctccata cgtaataata
      121 tcatgcgtat gtcttaattg tattaatttt tgatacgtat agaaaataga atctttattc
      181 tgaagtgctt gtctaacgtt gacctgatga taattttccg aaatatcaat ccaaggctta
      241 ccagctgtaa atccagcatt aacatcatca ctccattgca taggcgttct cgaattatct
      301 cgtgacttct gtcctaaaat cgctaacaca tccgcttcag catgcccttc acttaacaac
      361 tgatggtagg cattaatcga ttcaacatca cgatattgtg ctattgatgt aaaatgtggg
      421 tccgtcatac caatttcttc accttggtaa atatatggcg tcccttgcaa catatgcagt
      481 gcgatagcta acattttagc actttgtatc ctcatctctt ccgacgtatc atcaccaaat
      541 ctagacacta cccgtggctg atcatgatta caccagaaaa tcgcgttcca tccgccaccg
      601 tcataaatac ctcgttgcca ttgcatcaga atttccttta acttatgaaa atcaagcttc
      661 gcatttgtcc acttttcacc atcaacataa tcaaccttta gatgatgaaa attaaaaaca
      721 ctattcaatt cttggcgttc tggctgtgta tacttaatac aattttcaat cgtcgtcgaa
      781 gacatttctc ctacagtcat catgtcagtg ttaccaaacg tttgacgatt taattcatgc
      841 agaaactcat gcactctagg accatccgta taaaattctt tacctatttt gtcagagtcc
      901 ttaaattcac ctttagaaat taagttaatg acatcaaatc gaaaaccatc aacgccgaag
      961 tctatccaat gattgacaat gcgatataac gattgacgta cttccggatt atcccaattt
     1021 aaatcagctt gactgacatc aaataaatgt aaataatatt catctgtctc agaatcatac
     1081 ttccatgcat tacctccgaa tttggaatgc caatttgttg gaggcccatc ttcagatgct
     1141 ttaaaaaagt aataatctct atatggattg tctttagatt tacgagccgc tttaaaccat
     1201 tcatgctccg tcgatgtatg attgattaca atatctaaca tcactttaat acctttttca
     1261 tgcgcaacat taattaacct ttcaaaatca tccatcgtac cgaagtcctc atttatttct
     1321 aaataattgc ttatatcata accattatca ttcattggcg attcatacac tggtgttaac
     1381 caaatataat caacacctaa caatttgata taatctaatt tttctataat accgttgata
     1441 tcacctatac catttcctgt cgtatcatta aatgacttag gatatatttg atatactact
     1501 gactttctcc aatctatttc tttcaacaca cgatgaccac cttataacat aataaaaggc
     1561 gtccaaataa cgaacgcccc ttttttattt tattaatctt caacaatttc ttttgctttt
     1621 tgtttactaa aattagacat cacaatcgtt aatatacatg gtacaacaat tgcaattcct
     1681 gttacaacaa gatatacagg ccaaaattct ttttgaattg aaatgaatgc cggtacacca
     1741 ccaacaccaa cttttccaag tacattattc ataccaacga ttgcccctaa tacacaagac
     1801 gttgatattg cggcgataaa tggatatttt aaaggtaagt tcacaccaaa catggctggc
     1861 tccgtaacac ctaacatacc agaaatacaa gatgttaatg ctaaaccttc ttctttaacc
     1921 attttacgtc gtttatagac aaaccatgca ccaaatgctg ctgaaccttg acaaatattt
     1981 gagattgcaa caattggcca taagtatgta ccgcctaaac tgctacccat taattggaaa
     2041 tctactgcta aaaacatatg atgcaagcca gtaattacaa gcggtgcata tagcaaacca
     2101 taaattgcgc cacctaacca accagcatgt tggaatataa atgtaacacc agatgtaata
     2161 cctgtaccaa tcaataatgc aactggtcca atgataataa atgctaaaaa tccagtaact
     2221 aaaagcgcta cgggtccaac gaccaacatt tttatcgaat cgtgaacaac tttatttaat
     2281 cctttttcaa ttttagctag aacataagct gcaattaaaa caggcaacac ttgaccttgg
     2341 taattcaact gcttaatctc taagccaaat aaattccacg tcggaatatt accttttgcc
     2401 aaatcatact gagatactaa ttgcgggtgc attaaaatca aacctaagac taaacctaga
     2461 atcggactac caccaaatac acgcatacta ctccaaccaa ttaatgctgg taagaaaata
     2521 aatgccgtgt ttgctatgac gtttatcata tttgaaatat cagctatttg tggatacatc
     2581 acaatgagtt ctttcggacc aaataatcct ttcattgtaa gtaaattatt gatccccatt
     2641 aacaaaccag ctgtcacaat cgcaggtaat attggtataa aaatatcccc caacaattta
     2701 atcaaacgtt gtactggatt ccctttttgc gcagctgctt ttttcgcttc atctttagaa
     2761 gcttcttgag cacctgtttc atcaataaac tgcttataca cttcatcgac tgttcctgga
     2821 ccaatgacaa tttgatattg atggtctgct ttgaactgcc ctttaactaa agcattatta
     2881 cttaacgctt ccttatcaac tttactttca tctttaagta ctaaacgcaa tcttgttaca
     2941 caatgtgttg cagcttctag attatccttt cccccaattg cggtaacgat ggcttttaca
     3001 tcttctcttt ttacagccat gaacttcact cccttaatgt cttaaaaaga gtataacttg
     3061 tctagacaag ttatgtaaag gctttcatga aaatatttta atatttcaca attatctagt
     3121 ttagatattt aagaaaatca aatggctacg actacattta tagcatgaat tttcgctaca
     3181 atagcattat ccataacctc caaacgcata cactatgtta aagaataagc tgtattatca
     3241 tacccatacc caaatagtaa tttccatgcc caagcaaaga ctcctacgta catcaccaaa
     3301 taaaaaagac attcagccat gtgtcattca aaagccaaat gtcctttcta tttcaatgcc
     3361 ttatcatttc tatagccaaa aatacgaccc attcaaagac aaataaaaaa ggtaaatact
     3421 caaaatttga gcacttacct tcattatata tagtatttaa tataaatatt gatg
//
LOCUS       DAMZPP010000197         3239 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMZPP010000197 DAMZPP010000000
VERSION     DAMZPP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.198"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..686
                     /locus_tag="POH73_002655"
     CDS             <1..686
                     /locus_tag="POH73_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6888558.1"
                     /translation="DSDSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSESDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDAGKHTPVKPMSATKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLL
                     FGRRKKQNK"
     gene            1083..>3239
                     /gene="sdrE"
                     /locus_tag="POH73_002656"
     CDS             1083..>3239
                     /gene="sdrE"
                     /locus_tag="POH73_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046462978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrE"
                     /protein_id="HDI6888559.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDEASASDNKEVVSETENNSTQKNDLTNPIKKETNTDSHQ
                     EAKEAPTTSSTQQQQNNATTSTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNN
                     TNNDVTTKPSTSEIQTTPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKE
                     ELKNNPEKLKELVRNDSNTDRSTKPVATAPTSVAPKRVNAKIRFAVAQPAAVASNNVN
                     DLITVTKQMITEGIKDDGVIQAHDGEHIIYTSDFKIDNAVKAGDTMTVKYDKHTIPSD
                     ITDDFTPVDITDPSGEVIAKGTFDLNTKTITYKFTDYVDRYENVNAKLELNSYIDKKE
                     VPNETNLNLTFATADKETSKNVKVEYQKPIVKDESNIQSIFSHLDTTKHEVEQTIYVN
                     PLKLNAKNTNVTIKSGGVADNGDYYTGDGSTIIDSNTEIKVYKVASGQQLPQSNKIYD
                     YSQYEDVTNSVTINKNYGTNMANINFGDIDSAYIVKVVSKYTPGAEDDLAVQQGVRMT
                     TTNKYNYSSYAGYTNTILSTTDSGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSK
                     EKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPAGYLPTKENGT
                     TDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLG"
BASE COUNT         1307 a          515 c          564 g          853 t
ORIGIN      
        1 gcgactcaga ctcagacagc gactcagatt cagacagcga ctcagactca gatagtgatt
       61 cagattcaga cagcgactca gactcagaaa gcgactcaga ttcagacagc gattcagaat
      121 cggatagcga ctcagacagc gactcagact cagacagcga ctcagactca gatagcgact
      181 cagattcaga cagtgactca gatagcgact cagattcaga cagtgactca gacagtgatt
      241 cagactcaga tagcgattca gattcagaca gtgactcaga ctcagacagt gactcagatt
      301 cagatagcga ctcagattca gatagcgact cagactcaga tagcgactca gactcagata
      361 gtgactcaga ctcagacagt gactcagatt cagacagcga ttcagactca gatagcgact
      421 cagattcaga cagtgactca gactcagata gcgattcaga ttcagacagt gactcagact
      481 cagatagcga ttcagattca gatgcaggaa aacatacacc tgtgaaaccg atgagtgcta
      541 ctaaagacca tcacaataaa gcaaaagcat taccagaaac aggtagtgaa aataacggct
      601 caaataacgc aacgttattt ggcggattat tcgcagcatt aggttcatta ttgttatttg
      661 gtcgtcgtaa aaaacagaat aagtaatttg cttaaccaaa ccaggtccgt gaggcctggt
      721 tttttatttc aaaatttatg aaatgtatct tatgtttgag attaaatagt gcaatatttt
      781 aaaagatata acatacatca acataaattt aattcatctt tatattaaaa gtacagacaa
      841 ataagaaaaa caattaaaca cagtttcaaa ccatttaaaa caattagtat aaaaagtaaa
      901 tagataatta aaaatcaata aaattaagtt attcaaacca aagtgtaaaa taacagttga
      961 aaataaataa atttatttaa agtattgtgc tttatctaaa aatgtattac gatgggaata
     1021 caaattttta tatgtaaaaa tattagtttt ttgcaaatat tgatatttta aaggagattt
     1081 atatgattaa cagggataac aaaaaggcaa taacaaaaaa gggtatgatt tcgaatcgct
     1141 taaacaaatt ctcgattaga aagtatactg taggaactgc atcgatttta gtaggtacga
     1201 cattgatttt tggtctaggg aaccaagaag ctaaagctgc tgaaaacact agtacagaaa
     1261 atgctaagca agatgaggca tcggctagtg ataataaaga agtagtgtca gaaactgaaa
     1321 ataattcgac acaaaaaaat gatttaacaa atccaattaa gaaagaaaca aatactgatt
     1381 cacaccaaga agctaaagaa gcaccaacta catctagtac tcaacaacag caaaataatg
     1441 ctacaacttc aactgaaact aagcctcaaa acattgaaaa agaaaatgtt aaaccttcaa
     1501 ctgataaaac tgcgacagaa gatacatctg ttattttaga agagaagaaa gcgccaaata
     1561 atacaaataa cgatgtaact acaaaaccat ctacaagtga aattcaaaca acaccaacta
     1621 cacctcaaga atctacaaat attgaaaatt cacaaccgca accaacgcct tcaaaagtag
     1681 acaatcaagt tacagatgca actaatccaa aagaaccagt aaatgtgtca aaagaagaac
     1741 ttaaaaataa tcctgagaaa ttaaaagaat tagttagaaa tgatagcaat acagatcgtt
     1801 caactaaacc agttgctaca gctccaacaa gtgttgcacc aaaacgtgta aacgcaaaaa
     1861 tacgctttgc agttgcacaa ccagcagcag ttgcttcaaa caatgttaat gatttaatta
     1921 cagtgacgaa acagatgatt actgaaggta ttaaagatga tggtgttatt caagcacatg
     1981 acggtgaaca tattatttat acatccgatt ttaaaattga taatgctgta aaagctgggg
     2041 atacaatgac agtaaagtat gataagcata caattccttc agatattacg gatgatttca
     2101 caccagttga tattacagat ccatcaggtg aagtaatagc gaagggaaca tttgatttaa
     2161 atacgaaaac aattacttac aaatttactg attatgttga tagatatgag aatgtcaatg
     2221 caaaattaga actaaactca tatattgata agaaagaagt cccaaatgaa acaaatttaa
     2281 atttaacatt tgctacagca gataaagaaa ctagtaaaaa tgttaaagtt gaatatcaaa
     2341 aacctattgt aaaagatgag tcaaatattc aatctatatt tagccattta gatactacta
     2401 agcatgaggt agaacagact atttatgtga atccattaaa attaaatgct aaaaacacaa
     2461 atgttactat aaaaagtggt ggtgtagcag ataatggtga ttattatact ggagacggta
     2521 gtactataat tgatagtaat acggaaatta aagtctataa agttgcatcg ggtcaacaat
     2581 tacctcaaag taataaaatt tatgattata gtcaatatga agatgttacc aattcagtaa
     2641 ctattaataa aaattatggt actaacatgg ctaatattaa ttttggtgac attgattctg
     2701 catatatagt taaagttgtt agtaaatata caccgggtgc tgaggatgac ttagcagttc
     2761 aacagggtgt aagaatgact acaacaaata aatataatta ttcatcatat gcaggttaca
     2821 ctaatactat tttatcaact actgactctg gtggtggtga cggtactgtt aaacctgaag
     2881 aaaagttata caaaattggt gactatgtat gggaagacgt tgataaagac ggtgttcaag
     2941 gtacagattc aaaagaaaag ccaatggcaa acgttttagt tacattaact tacccggacg
     3001 gtactacaaa atcagtaaga acagatgcta acggtcatta tgaattcggt ggtttgaaag
     3061 acggagaaac ttatactgtt aaattcgaaa cgccagctgg atatcttcca acaaaagaaa
     3121 atggaacaac tgatggtgaa aaagactcaa atggtagttc tgtaactgtt aaaattaatg
     3181 gtaaagatga tatgtcttta gacactggtt tttataaaga acctaaatat aatcttggt
//
LOCUS       DAMZPP010000198         3220 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMZPP010000198 DAMZPP010000000
VERSION     DAMZPP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.199"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..328
                     /locus_tag="POH73_002657"
     CDS             <1..328
                     /locus_tag="POH73_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDI6888560.1"
                     /translation="LNPALKNIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSSIY
                     LLNRLNQGNSKGILIGLCAAMILLISISRVYLGVHYPTDIIAGIIGGVFCIILSTLLL
                     RNKLIN"
     gene            445..644
                     /locus_tag="POH73_002658"
                     /pseudo
     CDS             445..644
                     /locus_tag="POH73_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184337.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            720..852
                     /locus_tag="POH73_002659"
                     /pseudo
     CDS             720..852
                     /locus_tag="POH73_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1114..1773
                     /gene="arlR"
                     /locus_tag="POH73_002660"
     CDS             1114..1773
                     /gene="arlR"
                     /locus_tag="POH73_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HDI6888561.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            1770..3125
                     /gene="arlS"
                     /locus_tag="POH73_002661"
     CDS             1770..3125
                     /gene="arlS"
                     /locus_tag="POH73_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HDI6888562.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIDYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
BASE COUNT         1232 a          400 c          536 g         1052 t
ORIGIN      
        1 tttgaatcca gcattaaaaa atatattcga tagagaaaga cctacattgc tgcgtttaat
       61 tgatataaca ggatttagtt ttcctagcgg tcatgctatg ggatcaactg catattttgg
      121 aagtagtatc tatttattaa atcgattaaa tcaaggtaat tcaaaaggta ttcttatagg
      181 gttatgtgca gctatgattt tattgatttc catatcacgt gtatatctag gtgtacatta
      241 tccaacagat attattgccg gcattattgg tggcgtattt tgcattattt tatcaacgtt
      301 attacttaga aataaattaa tcaattaaat agtaaaaaaa caaaagcagt aaacctaaag
      361 tgtcgtaagg gtttactgct tttataaaac gttgttataa cgtatattgt cttttacggg
      421 catataaaag gggaatattt gaaaatgacc aatccaacaa gaacgaaacg ttgtgggggg
      481 atgttctatg tggtattgat aatcattttc aactactatt atacattagt gggaatcatt
      541 gtcaattaga aactaaaact ttttttgaat attttttaag aatagtaaat aaaacgcatg
      601 attacgctat tttagaaaat aaaaaaattt gtatttctca gtagaattag aatatttaaa
      661 agtgatgagg cttaaaaatt atattgttac atacttcttt tgaattcata cattaagaaa
      721 tgttacttgc aagttcaaac aggcacattg aaatgatgtg tgataaatgt ttaaactacg
      781 gtcattttgt gaaaataaag ataataacta ttcattttac attaataaaa gtcagtatac
      841 gacaacaatt gatattgcgg taagaccttg tgttacagta ttctatattt aagtactgca
      901 atcagaatta acagaattac attaactgat tattaaatat ttgagttaat aaataattaa
      961 tgattgtagc ttgaaaaatt taaaacatgg ttattgattt gtgataaaat ttaaacgtaa
     1021 acaaactaat ttaaaaagca actattgtat agaaaaatac aaaatttaaa atatattacc
     1081 ttattagaaa aagtcgtaat atgaggtgta caaatgacgc aaattttaat agtagaagat
     1141 gaacaaaact tagcaagatt tcttgaattg gaactcacac atgaaaatta caatgtggac
     1201 acagagtatg atggacaaga cggtttagat aaagcgctta gccattacta tgatttaatc
     1261 atattagatt taatgttgcc gtcaattaat ggcttagaaa tttgtcgcaa aattagacaa
     1321 caacaatcta cacctatcat tataattaca gcgaaaagtg atacgtatga caaagttgct
     1381 gggcttgatt acggtgcaga cgattatata gttaagccat ttgatattga agaactttta
     1441 gcaagaattc gtgcgatttt acgtcgtcag ccacaaaaag atattatcga tgtcaacggt
     1501 attacaattg ataagaatgc ttttaaagtg acggtaaatg gcgcagaaat tgaattaaca
     1561 aaaacggagt atgatttact atatcttcta gctgaaaata aaaaccatgt tatgcaacgg
     1621 gaacaaattt taaatcatgt atggggttat aatagtgaag tagaaacaaa tgtcgtagat
     1681 gtttatataa gatatttacg aaacaaatta aaaccatacg atcgtgacaa aatgattgaa
     1741 acagttcgtg gcgttgggta tgtgatacga tgacaaaacg taaattgcgc aataactgga
     1801 ttattgttac cacgatgatt acgtttgtca cgatattttt gttttgttta attattatct
     1861 ttttcttgaa agatacactg cataatagtg agcttgatga tgcagaaaga agctcaagcg
     1921 atattaataa tttatttcat tctaagcctg ttaaagatat atctgcatta gacttgaatg
     1981 catctttagg taattttcaa gagattatta tttatgatga gcataataat aaattatttg
     2041 agacatcgaa tgataacaca gtgagagttg aacctggtta tgaacaccgg tattttgacc
     2101 gcgtaataaa aaaacgttat aaaggcattg attatttaat tattaaagaa ccaatcacaa
     2161 cgcaagattt caaagggtat agtttgttaa ttcattcact agaaaattat gataacatcg
     2221 taaaatcatt gtatattatt gcgctcgcat ttggagtgat tgcaacaatt ataactgcca
     2281 caattagtta tgtattttca actcaaatta ctaaaccgct tgtcagttta tcaaataaaa
     2341 tgattgagat ccgacgagat ggttttcaaa ataaattgca attaaataca aattatgaag
     2401 aaatagataa tttagcaaat acgtttaatg agatgatgag ccaaattgaa gaatcattta
     2461 atcaacaaag acaatttgtt gaagatgcat cacatgaatt acgaacacca ttacaaatta
     2521 ttcaaggtca tttaaatttg attcagcgat ggggaaaaaa agacccagca gtattagaag
     2581 aatcgttaaa tatttctatt gaagaaatga atcgtatcat aaaattagtc gaagaattac
     2641 ttgaattgac taaaggagat gtaaatgaca tttcttctga agcgcagacc gtgcatatta
     2701 atgatgaaat tcgctcgcga atacactcat taaaacaatt gcatcctgat tatcaatttg
     2761 atacggatct gacatctaaa aatctagaaa ttaaaatgaa acctcatcaa tttgaacaat
     2821 tatttttaat ctttattgat aatgcaatta aatatgatgt gaagaataag aaaattaaag
     2881 ttaagacaag gttaaaaaat aagcaaaaaa taattgaaat tacagatcat ggaattggta
     2941 ttccagagga agatcaagat ttcatttttg atcgctttta tcgagtggat aaatctcgtt
     3001 caagaagtca aggtggtaat ggactcggat tatctattgc gcaaaaaatc attcaattaa
     3061 acggtggatc gattaaaatt aaaagtgaga ttaacaaagg aacaacgttt aaaatcatat
     3121 tttaatcatg tttaagacgt caatcaaagt catagaatca attttttaag tgaggctggg
     3181 acataaatca atgttctatg ctttacgaag ttatattggc
//
LOCUS       DAMZPP010000199         3174 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMZPP010000199 DAMZPP010000000
VERSION     DAMZPP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.200"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            606..1496
                     /locus_tag="POH73_002662"
     CDS             606..1496
                     /locus_tag="POH73_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDI6888563.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQIVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEIK"
     gene            1745..2794
                     /locus_tag="POH73_002663"
     CDS             1745..2794
                     /locus_tag="POH73_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDI6888564.1"
                     /translation="MFLAWNEIRRKKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASPVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTLILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            2807..>3174
                     /locus_tag="POH73_002664"
     CDS             2807..>3174
                     /locus_tag="POH73_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6888565.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIVALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQL"
BASE COUNT         1210 a          432 c          555 g          977 t
ORIGIN      
        1 aaataataac ttgttttttc aatgtaattt ccttcctaaa actagatatt cataataatt
       61 taaaaaatct gaaaaataaa aacgttctta ctttatcttt aaatgacaat actatgattc
      121 tattatttta taaaaaagat tgcaataata acgtcttgct attattggat aatgacaatt
      181 aaagtgtcgt ttttatgaat taaaataggt gaaagtaagt tggaaatacg ttggagcgtt
      241 gtgattttta tgagaaattg tatgagtgag gactgctatt tataaaaggt aaactatgga
      301 tgagtttatg gttgttccaa taggtaaaca agagatagca cactgtacac ataatgatac
      361 gtggatgagt gaatatgact gataggaagg cctagtcact caaatgaatt aaagtttatt
      421 acgtgataaa tcacaaatct ctctcatgtg ataggtctcc cattaaatca tgcttatatg
      481 aaatgttcac atatttgtta gcttttcaag aaataatatt aaatatcttt tcatagtata
      541 gcaatattaa ataaatgcat ttataatttt aactgtattt taaatattaa tcatgaggtg
      601 ataagatgaa taaaatttca aagtatattg caatagcatc attatcggta gcggttacag
      661 tttcggcacc acaaacgaca aattctacag cgtttgccaa aagttctgct gaagttcaac
      721 aaacacaaca agcttctata ccagcatcac aaaaggcgaa tcttggtaat caaaatatta
      781 tggcagtggc ttggtatcaa aattcagctg aagcaaaagc attatattta caaggttata
      841 acagtgcaaa gactcagtta gataaagaga ttaaaaagaa taagggtaaa cataagttag
      901 ctattgcttt ggatttagat gaaacagttt tagataattc tccatatcaa ggctatgcat
      961 caatacacaa taaacctttc ccagaaggtt ggcatgaatg ggtacaagct gctaaagcta
     1021 aacctgtcta tggcgcaaaa gaattcttga aatatgctga caaaaaaggt gtcgatatct
     1081 actatatttc tgatagagat aaagaaaaag atttaaaggc aacacaaaag aacttaaaac
     1141 aacaaggtat ccctcaagct aagaagagtc atattttact aaaaggtaaa gatgataaga
     1201 gtaaagaatc acgcagacaa atagttcaaa aggatcataa acttgtcatg ctatttggag
     1261 ataatttatt agactttaca gatccaaaag aagctacagc tgaatctcgt gaagcattaa
     1321 ttgaaaaaca taaagacgat ttcggtaaga aatatatcat tttccctaac ccaatgtatg
     1381 gtagttggga agctacgatt tacaacaata actataaagc aagtgacaaa gcaaaagata
     1441 aattacgtaa aaatgctatt aagcaattcg atcctaaaac aggcgaaatt aaataatata
     1501 tgaattggac gtctacatgt acttaaaggt atatgtaggc gtttttaaat tgatccttaa
     1561 ttttaaatgt accgtacaaa aataatagac aattaattgc attttccata aatttgcttt
     1621 taatataaaa agttcgggta taatgaagga taagtgtatg tataacatta cagagcgcaa
     1681 cacgacgtac gtgcatctaa gaatataaac aatgtttaaa tcatagtagt agggagagaa
     1741 atgcatgttt ttagcttgga atgaaatacg gcgcaaaaaa ttgaagtttg gactaattat
     1801 tggtgtgtta acgatgatta gttacttgct atttttatta tctggattgg cgaatggtct
     1861 tatcaatatg aataaagaag gcattgataa gtggcaagca gatgccattg ttctaaataa
     1921 agatgccaat caaactgtgc aacaatctgt ttttaacaag aaagatattg aaaataaata
     1981 caagaaacaa gctacattga agcaaacagg agaaatagtt tctaatggtc atcaaaaaga
     2041 caatgtctta gtgttcggtg ttgaaaagtc atcattttta gttccgagtt taatagaagg
     2101 gcataaagcg actaaagata atgaagtgtt agctgatgaa acacttaaaa ataaaggatt
     2161 taaaattggc gacacattat cactatctca atcagatgaa aaattgcata tcgtaggttt
     2221 tacagaaagt gcaaaatata atgcgtcacc agtcattttc acgaatgacg ctaccattgc
     2281 caaaatcaat cctagattga ctggagataa aattaatgca gttgttgtac gtgatacaaa
     2341 ttggaaagac aaaaaattaa accaagagct tgaagcggta agtattaatg actttattga
     2401 aaatttacca ggttataaac cacagaactt aacattaaac tttatgattt cattcttatt
     2461 tgtcatttca gctacagtta taggtatatt cttatatgtc atgacattac aaaaaacaag
     2521 tttatttggc atattaaagg ctcaaggatt tacgaatggc tatttagcga acgtagttat
     2581 ttcacaaacg ctcatattag cattatttgg tacggcattt ggcttactgt taacaggcgt
     2641 tacaggtgca tttttacctg atgcagtacc tgtcaaattc gatgtactaa cattgctcgt
     2701 atttgcaatt gtgttaatga ttgtctctgt attaggaagt ttattctcca ttttaacaat
     2761 tagaaaaata gatccgttaa aggcgattgg gtaggaggtg tagcaaatgt tgaaatttga
     2821 aaatgtaaca aagtcattta aagatgggaa tcgtaacatt gaagcggtta aagatacaaa
     2881 ttttgagata aataaaggtg atattgtagc attagttgga ccttctggct ctggtaaaag
     2941 tacatttcta actatggcag gtgctttaca aacaccgaca tctgggcaca ttttaatcaa
     3001 taaccaagat attacgacaa tgaagcaaaa agcattggca aaagttagaa tgtctgagat
     3061 aggttttatt ttacaagcta caaatcttgt accattttta acggtaaagc aacaatttac
     3121 attattgaaa aagaaaaata agaatgttat gtctaatgaa gactatcagc aact
//
LOCUS       DAMZPP010000200         2930 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMZPP010000200 DAMZPP010000000
VERSION     DAMZPP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.202"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..217)
                     /locus_tag="POH73_002665"
     CDS             complement(<1..217)
                     /locus_tag="POH73_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDI6888566.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILTENPNA"
     gene            complement(201..1085)
                     /gene="ylqF"
                     /locus_tag="POH73_002666"
     CDS             complement(201..1085)
                     /gene="ylqF"
                     /locus_tag="POH73_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDI6888567.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKAHYKIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            1486..>2930
                     /gene="yfhO"
                     /locus_tag="POH73_002667"
     CDS             1486..>2930
                     /gene="yfhO"
                     /locus_tag="POH73_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HDI6888568.1"
                     /translation="MLKKWLNSNVKQFLVITFMSVILTLILFSTHIYEYIVNGTVFSG
                     SGDGFRQMMPFQMYLYEHLSSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMVRAIITFVVTFYLFKILHFKRSTNMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFFITISILTIVALLSFKLYRFYFYRLFAIVTWVLF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVSI
                     IALLYVLLSPTHPLALIVGIILQMVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSSLKQHDYHSNYVNQLI"
BASE COUNT          878 a          511 c          379 g         1162 t
ORIGIN      
        1 tagcattagg attctctgtt aatatttcat tttcaaagta agtcatttca acatactttt
       61 cttttaaagc ttgttctttt tctaacgctt ttcttcgtct ggctatggca ttttgtacac
      121 cttttcgctc atctaaaaag cattcatgat tttctaattc ttctattgta ttaaccgcat
      181 taatcaactg cgttacttct ttaattgtta gcgtcatttg ctaattcctc agtcatatct
      241 ttaaaaatat caaaacaata atttcctatt ttagcatttc gaatatcata aataatcagt
      301 tcaatgactg cttcgtaatc aatttcatta ccacgtcgaa ttaagccacg ttttttccct
      361 atcgcatcaa accacgctat gatttctgca tcttcaggaa cttcgatttt ataatgtgct
      421 tttaagcgcg ctaaatcatt ttgaattaaa aagttcagtc catagatggc aacttcatct
      481 aagtgcacaa tactatcttt aatcgcgcct gttaaactca acttcttacc gacctcttca
      541 tcttcaaatt taggccaaag aatccctggt gtatctaata gttgtaatgc attaccaact
      601 ttaatccatt gttgttgttt ggtcacacct ggtttattac cagtctgtgc aatactacgc
      661 tttgccagtt tatttattaa tgtggattta ccaacatttg gaattccaac aatcatcgct
      721 ctaatcgctc ttggcttaag tccttttgct ttttcacgtt caaatttttc agcagtcgcc
      781 ttaattgctg cagcttccac tttctttaaa tttttaccgt gcttagcatc cactgataca
      841 ggatagtatc ctttatcaat aaaaaattgt tcccattttg acatctcatt taaattagac
      901 atatcttttt tatttaatat aacaacacgt ggtttttggt taataacttc atctatcata
      961 gggtttcttg aactatatgg aattcttgca tctactagtt caaacactac atctactttt
     1021 tttaattgtt cacttacttc tcttttggct ttcgccatat gtcctggata ccattgaata
     1081 accattcaat tcaccttact ttcgtcaatt cttttctcat tattaaaatc tataacttgt
     1141 taattataga acaaattcat taattcaact atatttattt taacattgtc acacttctta
     1201 acaatgcttt agattaaggt ataagtgatt attccaagtt ttctctaatt aaaaattatc
     1261 atttattaaa tctaagctgc ttattaggtg atattcaatt aatacagtat gacattttta
     1321 ttattcaata tatttgttta atgataaact aaaaataatc caaagtttaa gtaactatga
     1381 taaggtatct gttccacagt catctttgtc tttacctatc gagaacctca ttaactttag
     1441 tttcagttaa acttaatttt aaatttgaaa gttgaggata atgttgtgtt aaaaaaatgg
     1501 ttaaattcaa acgtcaaaca attcttggtc ataactttca tgagcgtaat attaacgctt
     1561 attttatttt ctactcatat ctatgaatat attgtgaatg gtactgtttt tagtggatct
     1621 ggagatggat tccgtcaaat gatgccattt caaatgtatt tgtatgaaca tctaagtagt
     1681 ttttctagtt tatatgatgc atcgtttgga ttaggtggcg attatatgaa aggactatca
     1741 tattattatt cgctgtcacc tttaatgtgg ctcaattttc tattcattaa aataggagaa
     1801 actgttggta tatttaatcc gacaacaata catttttggc cgacaaacca acttattatg
     1861 gctatggtac gagctatcat aacatttgtc gtgaccttct acttatttaa aatattacac
     1921 tttaaacgct caacaaatat gatcgctacg attttatacg gcatgtcaac tgtcgttata
     1981 tactttaatt ttacttggtc attttatgga aatttattat atttattgcc attatcgatt
     2041 cttggtttgg aaagatattt tcaacaacgc aaaatcggta ttttcattgt tgcgatagcc
     2101 ttaacactat ttagcaattt ttatttcagt tattatcaag ctattattat aggttgctac
     2161 tatttatatc gactcatttt cacttacaaa tatgacattg tctctagaac acaaaaatta
     2221 atttgcgtca tatctgctac agttttgagt gtgttatcaa gtgtatttgg tttattcact
     2281 ggcatttctg cgtttttgga aaatgacaga aagcaaaatc ccaatgttga tataccgttt
     2341 ttgacaccac ttgattatca ttattttttc tttagcgatg gatttttcat tacgatttca
     2401 attctcacta tcgttgcatt attgtcattc aaactgtatc gtttttactt ttatagactt
     2461 ttcgcaatag taacatgggt attatttatt ggttcattat cacagtattt tgacagtgct
     2521 tttaatggtt tttcatttcc agaaaggcgt tgggtgtata tcttagcact atcatcaagt
     2581 gctctttgcg gattgtttat tcaacattta tcaacattaa atatgaaata ttatttaatc
     2641 agaacaatac cagtaagcat catcgcatta ctttatgtat tactatcacc gacacaccca
     2701 ctcgcactta tagtaggtat tatcctgcaa atggtgcttg ctgttatttt aaaatttagt
     2761 ttatggcgtt ataaaaaatt aaccgttgca atattagtat taatcgttat gattcaacaa
     2821 atcgtcattt tagataacaa caaaaatatg gcaatcaaac cttatcaaca atcattatca
     2881 tcgttgaaac aacatgatta ccatagtaac tatgtaaacc agcttataaa
//
LOCUS       DAMZPP010000201         2925 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMZPP010000201 DAMZPP010000000
VERSION     DAMZPP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.203"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..293)
                     /locus_tag="POH73_002668"
     CDS             complement(<1..293)
                     /locus_tag="POH73_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDI6888569.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAICGIFAFLIWI
                     HLLCTMLKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINNL"
     gene            540..737
                     /locus_tag="POH73_002669"
     CDS             540..737
                     /locus_tag="POH73_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6888570.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANGIFSDENYEE"
     gene            complement(1199..1272)
                     /locus_tag="POH73_002670"
     tRNA            complement(1199..1272)
                     /locus_tag="POH73_002670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1236..1238),aa:Arg,seq:tct)
     gene            1636..1770
                     /locus_tag="POH73_002671"
     CDS             1636..1770
                     /locus_tag="POH73_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDI6888571.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            1894..2589
                     /locus_tag="POH73_002672"
     CDS             1894..2589
                     /locus_tag="POH73_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDI6888572.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFYYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHNLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNEIVR"
     gene            complement(2697..>2925)
                     /locus_tag="POH73_002673"
     CDS             complement(2697..>2925)
                     /locus_tag="POH73_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6888573.1"
                     /translation="YFPDFEDYILLWKLEVLPKYQNRGYASELIDFAKSFNMPIKAIG
                     RNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
BASE COUNT         1085 a          384 c          498 g          958 t
ORIGIN      
        1 attaaattat tgataaatgt tatattacta aaaaatgtat ttaaataagt agtacctaaa
       61 aagccagaca tgaaaaatgt tgtgaacaca gatgaaacta gagggctgtt taattgttct
      121 ttcacattat taaaatattt gagcatagta cataaaaggt gaatccaaat caagaaagca
      181 aagattccgc aaatagcgtt taaagtaaga gataagtctt ttaatagatt acccaggcct
      241 aataatccta agactagtcc tgacgttact agaggtgctt tttgaagtct catgatttac
      301 aacctttctt atgtgatttt cttcactaat tatatcatga ttgctatgga ctattaataa
      361 gaagagagtg tactattacg tttttagagt gtgtatattg gattgataga cacataattg
      421 acatttaaat ctcaaaatta atgatatttg aggtatgctt tgctacctaa atatggaatg
      481 tgggaaaagt atttatttaa tttaaatgat gattgaagat ttacaaggga gtgtacaaaa
      541 tgagaaatca aattcaaaaa ctattagaca gtgatttgag cagtttacat atatcaaaac
      601 aaacaggagt gccacaaagc acaatacaca gaatgagaaa aaatgaaaga tcattagaca
      661 atatgtcatt gaaaaacgct gaactacttt ataaatttgc caatggtata tttagtgatg
      721 aaaattatga agaataatta gtgagttaaa taattagaaa accacgtctc gatggacgtg
      781 gttatttttc agatgtatat aattataatt ataggatttc gggtccctga aaagtcccta
      841 aaattttgaa tagtatcaaa atctatagga ataatataat aataaaaatc tctacgcatg
      901 caaatggtga gtgttaaaaa tcttggtgta gttttggtgt agttcaggta cagtatagag
      961 gaaatttgag cgcaaaatta cttaattaaa aatgtgtaaa caagtgtcag acgcgtgtca
     1021 attgtgtgtc aaaaaattct ataacgaata tgcatgcttg tttttatcac tgtattttct
     1081 gggcttgtgc ggggacgttt tggtgacgca tcatactata ttactgaaat tcaaaacaaa
     1141 aagagccccg taatcacgga actcttttgt ttggtaatgc gtataaaaat actataaacg
     1201 tcctgggagg gattcgaacc cccgaccgat ggcttagaag gccattgctc tatccagctg
     1261 agctaccagg acacgtttaa caacacaaga attattatat ctaaatgaac ttaaattagc
     1321 aataccttgt aaataaaaaa tgtttatatt tttcactatt atagagctat ttatctaaaa
     1381 aggttcaata agacttaaat acgaattcag gcaacttaat tgtgttaaat acagttttga
     1441 atgcctaact gtatatcttt tctctttaaa gtacagttaa gtacattata agatgttgtg
     1501 cggataaaca aactaattgc atcaaattta ttttaaaata acaacaacaa aacgttgagc
     1561 gaataacatt tcggtgattt aaaagctacg cacgtttttg ttatcttcaa atttaaattt
     1621 taaggagtgt tttcaatgga aggtttattt aacgcaatta aagataccgt aactgcagca
     1681 attaataatg acggcgcaaa attaggcaca agcattgtga gcatcgttga aaatggcgta
     1741 ggtttactag gtaaattatt cggattctaa tataataact aatattcttt aaaataaact
     1801 gggtgagcat actttaatgt tatgcactca gtttatttta tttgcagaaa tttgagcctc
     1861 tgttaagatt tagatacata gacaatatag gagatgggga aattgggata taaaaatatt
     1921 ttgatagact ttgatgatac aattgttgat ttttatgatg cagaagaatg ggcattttac
     1981 tatatggcga atgtttttaa tcataaagca acaaaggatg attttttaac atttaaaaaa
     2041 atcaatcacc aacattggga agcttttcaa caaaataaat taacgaagtc tgaagtatta
     2101 tcagaacgat ttgtaaatta cttcaaacat catcaaatgg aagttgatgg gcatcgtgca
     2161 gatgtgttat ttagaaatgg attagcagaa gctaaagtta aatactttga tcaaacatta
     2221 gaaacaattg tcgagttatc gaaaagacat aatctatata ttgttactaa tggtgtaaca
     2281 gaaacgcaaa agagaaggtt aaatcagacg ccattgcata aatatattaa aaagatattt
     2341 atatctgagg aaacaggata tcaaaaacct aatccggaat tttttaatta tgtttttaat
     2401 gatattggtg aggatgaaag acagcactcg attatagttg gagattcttt aacatctgac
     2461 attctaggtg gaatcaatgc gggtatagct acttgctggt ttaattttag aggatttgat
     2521 cataatccag gaattatacc tgattatgaa attaattcat ggaaacaact aaatgaaatt
     2581 gtacgttaag acaaataatt tgaaaatgat ttacttatat tagctaagaa agtaattgta
     2641 aaaaagacac tacgaaaaga tgcattttca ttctcgtagt gtcgaatatt attatcttat
     2701 ggtttccaca ataagacatc atgtccctct atatttttag cttccacatc tgtaaatcca
     2761 tgatgtaaaa agaaatcctt agaatcattt ctgccaatgg ctttaattgg catattgaaa
     2821 ctctttgcaa aatcaatcaa ttctgaagcg taccctctgt tttgatattt tggtaatact
     2881 tctaacttcc ataataatat ataatcttca aaatctggga agtag
//
LOCUS       DAMZPP010000202         2923 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMZPP010000202 DAMZPP010000000
VERSION     DAMZPP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.204"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..2912
                     /locus_tag="POH73_002674"
     rRNA            <1..2912
                     /locus_tag="POH73_002674"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          809 a          597 c          863 g          654 t
ORIGIN      
        1 taagggcgca cggtggatgc cttggcacta gaagccgatg aaggacgtta ctaacgacga
       61 tatgctttgg ggagctgtaa gtaagctttg atccagagat ttccgaatgg ggaaacccag
      121 catgagttat gtcatgttat cgatatgtga atacatagca tatcagaagg cacacccgga
      181 gaactgaaac atcttagtac ccggaggaag agaaagaaaa ttcgattccc ttagtagcgg
      241 cgagcgaaac gggaagagcc caaaccaaca agcttgcttg ttggggttgt aggacactct
      301 atacggagtt acaaaggacg acattagacg aatcatctgg aaagatgaat caaagaaggt
      361 aataatcctg tagtcgaaaa tgttgtctct cttgagtgga tcctgagtac gacggagcac
      421 gtgaaattcc gtcggaatct gggaggacca tctcctaagg ctaaatactc tctagtgacc
      481 gatagtgaac cagtaccgtg agggaaaggt gaaaagcacc ccggaagggg agtgaaatag
      541 aacctgaaac cgtgtgctta caagtagtca gagcccgtta atgggtgatg gcgtgccttt
      601 tgtagaatga accggcgagt tacgatttga tgcaaggtta agcagtaaat gtggagccgt
      661 agcgaaagcg agtctgaata gggcgtttag tatttggtcg tagacccgaa accaggtgat
      721 ctacccttgg tcaggttgaa gttcaggtaa cactgaatgg aggaccgaac cgacttacgt
      781 tgaaaagtga gcggatgaac tgagggtagc ggagaaattc caatcgaacc tggagatagc
      841 tggttctctc cgaaatagct ttagggctag cctcaagtga tgattattgg aggtagagca
      901 ctgtttggac gaggggcccc tctcgggtta ccgaattcag acaaactccg aatgccaatt
      961 aatttaactt gggagtcaga acatgggtga taaggtccgt gttcgaaagg gaaacagccc
     1021 agaccaccag ctaaggtccc aaaatatatg ttaagtggaa aaggatgtgg cgttgcccag
     1081 acaactagga tgttggctta gaagcagcca tcatttaaag agtgcgtaat agctcactag
     1141 tcgagtgaca ctgcgccgaa aatgtaccgg ggctaaacat attaccgaag ctgtggattg
     1201 tcctttggac aatggtagga gagcgttcta agggcgttga agcatgatcg taaggacatg
     1261 tggagcgctt agaagtgaga atgccggtgt gagtagcgaa agacgggtga gaatcccgtc
     1321 caccgattga ctaaggtttc cagaggaagg ctcgtccgct ctgggttagt cgggtcctaa
     1381 gctgaggccg acaggcgtag gcgatggata acaggttgat attcctgtac cacctataat
     1441 cgttttaatc gatgggggga cgcagtagga taggcgaagc gtgcgattgg attgcacgtc
     1501 taagcagtaa ggctgagtat taggcaaatc cggtactcat taaggctgag ctgtgatggg
     1561 gagaagacat tgtgtcttcg agtcgttgat ttcacactgc cgagaaaagc ctctagatag
     1621 aaaataggtg cccgtaccgc aaaccgacac aggtagtcaa gatgagaatt ctaaggtgag
     1681 cgagcgaact ctcgttaagg aactcggcaa aatgaccccg taacttcggg agaaggggtg
     1741 ctctttaggg ttaacgccca gaagagccgc agtgaatagg cccaagcgac tgtttatcaa
     1801 aaacacaggt ctctgctaaa ccgtaaggtg atgtataggg gctgacgcct gcccggtgct
     1861 ggaaggttaa gaggagtggt tagcttctgc gaagctacga atcgaagccc cagtaaacgg
     1921 cggccgtaac tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacccg
     1981 cacgaaaggc gtaacgattt gggcactgtc tcaacgagag actcggtgaa atcatagtac
     2041 ctgtgaagat gcaggttacc cgcgacagga cggaaagacc ccgtggagct ttactgtagc
     2101 ctgatattga aattcggcac agcttgtaca ggataggtag gagcctttga aacgtgagcg
     2161 ctagcttacg tggaggcgct ggtgggatac taccctagct gtgttggctt tctaacccgc
     2221 accacttatc gtggtgggag acagtgtcag gcgggcagtt tgactggggc ggtcgcctcc
     2281 taaaaggtaa cggaggcgct caaaggttcc ctcagaatgg ttggaaatca ttcatagagt
     2341 gtaaaggcat aagggagctt gactgcgaga cctacaagtc gagcagggtc gaaagacgga
     2401 cttagtgatc cggtggttcc gcatggaagg gccatcgctc aacggataaa agctaccccg
     2461 gggataacag gcttatctcc cccaagagtt cacatcgacg gggaggtttg gcacctcgat
     2521 gtcggctcat cgcatcctgg ggctgtagtc ggtcccaagg gttgggctgt tcgcccatta
     2581 aagcggtacg cgagctgggt tcagaacgtc gtgagacagt tcggtcccta tccgtcgtgg
     2641 gcgtaggaaa tttgagagga gctgtcctta gtacgagagg accgggatgg acatacctct
     2701 ggtgtaccag ttgtcgtgcc aacggcatag ctgggtagct atgtgtggac gggataagtg
     2761 ctgaaagcat ctaagcatga agcccccctc aagatgagat ttcccaactt cggttataag
     2821 atccctcaaa gatgatgagg ttaataggtt cgaggtggaa gcatggtgac atgtggagct
     2881 gacgaatact aatcgatcga agacttaatc aaatttaaat gtt
//
LOCUS       DAMZPP010000203         2885 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMZPP010000203 DAMZPP010000000
VERSION     DAMZPP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.205"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            217..>2885
                     /locus_tag="POH73_002675"
     CDS             217..>2885
                     /locus_tag="POH73_002675"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDI6888574.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGIISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPAELALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKV"
BASE COUNT         1015 a          469 c          557 g          844 t
ORIGIN      
        1 gaacgactat aaaacaaata atcaggggtt ctttatcatt tctgtggaat tcacctccca
       61 aaatttacaa taaaatacat ctataaacct agaagaatca acgcttttgt tgattcttct
      121 ttttagtaga taaataggta aatctacttt aacaaataac taaatagtga tattattaca
      181 ttgtaagcgt ttcaacattt ttgtggaggg tgtaaaatga ctaacgaaag aaaagaagtt
      241 tcagaggctc ctgtaaactt cggtgcgaat ttaggtctaa tgttagatct atatgatgac
      301 tttttacaag atccatcatc tgtaccagaa gatttacaag tcttattcag cacaattaag
      361 aatgatgact caattgtacc agctttaaaa agtacaagta gtcaaaatag cgacggcaca
      421 attaagcgtg tcatgcgttt gattgataat attcgccaat acgggcatct taaagccgat
      481 atttatcctg taaatcctcc gaaaaggaaa catgtaccta aattagagat tgaagacttt
      541 gatttagatc aacagacttt ggaaggtata tcagcaggaa ttgtttcaga tcactttgcc
      601 gacatttatg ataatgctta tgaagcaatt ttaagaatgg aaaaacgtta caaaggacca
      661 attgcatttg agtatacaca tattaataac aataccgaac gtggttggtt aaaaagaaga
      721 attgaaacgc catataaagt aacgttaaat aataacgaaa aaagggcact atttaaacaa
      781 ttagcgtatg ttgaagggtt tgaaaaatat cttcataaaa acttcgttgg tgcaaagcgt
      841 ttttcaattg aaggggtaga cgcacttgta ccgatgttac aacgtactat tacgattgct
      901 gcgaaagaag gtattaaaaa tatacaaata ggcatggctc accgtggacg tttaaacgtt
      961 ttaacgcatg tcttagaaaa accgtacgaa atgatgattt cagaatttat gcatacagat
     1021 ccaatgaaat tcttacctga agatggtagc ttgcagttaa ctgctggatg gactggtgat
     1081 gtgaaatatc accttggtgg cattaaaact actgattcat acggtacaat gcagcgtatt
     1141 gcactggcta acaatccaag tcacttggaa attgttgcac ctgttgttga agggcgtacg
     1201 agagcagcac aagatgatac gcagcgtgct ggggctccga cgactgatca tcataaagca
     1261 atgccaatta ttatacacgg cgatgctgct tatcctggtc aaggtattaa cttcgaaaca
     1321 atgaacttag gaaacttgaa aggctattct acgggtggtt cattgcatat tattactaac
     1381 aatagaattg gatttaccac agaaccaatt gatgcacgtt caacaactta ttctacagat
     1441 gtggctaaag gttatgatgt gccaatattc catgtcaatg cagatgacgt tgaagctact
     1501 attgaagcaa ttgatattgc aatggaattt agaaaagagt ttcataaaga cgtcgttatt
     1561 gatttagtag gttatcgtcg tttcggacat aacgaaatgg atgaaccatc aattactaat
     1621 ccagttcctt atcagaatat tcgcaaacat gactctgttg aatatgtgtt tggtaaaaag
     1681 cttgttaatg aaggtatcat ttcagaagat gaaatgcatt catttataga acaagtccaa
     1741 aaggaactaa gacaagcaca tgataaaatt aataaagctg ataaaatgga taatccagat
     1801 atggaaaaac ctgcagaact tgcattgccg ttacaagcag acgaacaatc atttacgttt
     1861 gatcacttga aagaaataaa tgatgcattg ttaacatatc cggatggctt taacattttg
     1921 aaaaagttaa acaaagttct tgagaagcgt catgagccgt ttaataaaga agatggttta
     1981 gttgattggg cacaagcaga acaacttgca tttgcgacaa tcttacaaga tggtacaccg
     2041 attcgcttaa ctggtcaaga tagcgaacgt ggtacattca gtcataggca tgccgtgtta
     2101 catgatgagc aaacaggtga aacatataca cctttacatc atgttcctga tcaaaaagcg
     2161 acatttgata tacacaattc tccgctttca gaagcagcag tagttggttt tgaatacggc
     2221 tataatgtgg aaaacaaaaa aagcttcaat atttgggaag cgcaatatgg tgattttgca
     2281 aatatgtcac aaatgatttt tgacaacttc ttattcagtt ctcgctcaaa atggggagaa
     2341 cgttcaggat taacattatt cttacctcat gcatatgagg gtcaagggcc tgaacattca
     2401 tcagcaagat tagagagatt tttacaatta gctgctgaaa ataattgcac agttgtcaac
     2461 ttatctagtt caagtaatta tttccactta ttgcgtgcac aagcagctag tttagattct
     2521 gaacaaatgc gaccattggt tgttatgtca ccaaaaagct tactgagaaa taaaacagtt
     2581 gcaaaaccaa ttgatgaatt tacttctggt ggatttgagc caattttgac agaatcatat
     2641 caagcggata aagttacaaa agttattttg gcaactggca aaatgttcat tgatttaaaa
     2701 gaagcattag ctaaaaatcc agacgaatca gtattactcg ttgcgattga aagattgtat
     2761 ccattcccag aggaagagat tgaagcattg ctagcacaat taccaaacct tgaagaagtg
     2821 tcatgggtac aagaagaacc taaaaatcaa ggtgcatggt tatatgttta tccatatgtt
     2881 aaagt
//
LOCUS       DAMZPP010000204         2797 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMZPP010000204 DAMZPP010000000
VERSION     DAMZPP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.206"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..1595)
                     /gene="cna"
                     /locus_tag="POH73_002676"
     CDS             complement(<1..1595)
                     /gene="cna"
                     /locus_tag="POH73_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen adhesin Cna"
                     /protein_id="HDI6888575.1"
                     /translation="MNKNVLKFMVFIMLLNIITPLFNKNEAFAARDISSTNVTDLTVS
                     PSKIEDGGKTTVKMTFDDKNGKIQNGDTIKVAWPTSGTVKIEGYSKTVSLTVKGEQVG
                     QAVITPDGATITFNDKVEKLSDVSGFAEFEVQGRNLTQTNTSDDKVATITSGNKSTNV
                     TVHKSEAGTSSVFYYKTGDMLPEDTTHVRWFLNINNEKRYVSKDITIKDQIQGGQQLD
                     LSTLNINVTGTHSNYYSGPNTITDFEKAFPGSKITVDNTKNTIDVTIPQGYGSLNSFS
                     INYKTKITNEQQKEFVNNSQAWYQEHGKEEVNGKAFNHTVHNINANAGIEGTVKGELK
                     VLKQDKDTKAPIANVKFKLSKKDGSVVKDNQKEIEIKTDANGIANIKALPSGDYILKE
                     IEAPAPYTFDKDKEYPFTMKDTDNQGYFTTIENAKEIEKTKDVSAQKVWEGTQKVKPT
                     IYFKLYKQDDNQNTTPVDKAEIKKLEDGTTKVTWSNLPENDKNGKTIKYLVKEVNAQG
                     KDTTPEGYTKKEDGLVVTNTEKP"
     gene            1973..>2797
                     /locus_tag="POH73_002677"
     CDS             1973..>2797
                     /locus_tag="POH73_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDI6888576.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDTVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDESSRKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIG"
BASE COUNT          790 a          510 c          398 g         1099 t
ORIGIN      
        1 attggttttt cagtattagt aaccactaaa ccatcttctt ttttagtata tccttctggt
       61 gttgtatctt taccttgagc atttacttct ttaactaaat atttaatagt cttgccattt
      121 ttgtcatttt ccggaagatt agaccatgtc acttttgtcg ttccatcttc taatttttta
      181 atttctgctt tgtctactgg tgttgtgttt tgattgtcat cttgtttgta caacttgaaa
      241 taaatcgttg gtttcacttt ttgagtgcct tcccaaacct tttgagcaga aacatctttt
      301 gttttttcta tctcttttgc attttcaata gtcgtaaaat atccctgatt atctgtatct
      361 ttcatagtaa acggatattc tttatcctta tcaaatgtat acggtgctgg cgcctctatt
      421 tcttttaaaa tatagtctcc gctaggcaac gctttaatat tagcaatacc gtttgcatct
      481 gttttaatct caatttcttt ttgattgtcc tttacaactg atccatcttt tttagaaagt
      541 ttaaatttta cattagctat aggagccttg gtatctttat cctgttttaa aacttttaat
      601 tcacctttta cagtaccttc aataccggca ttagcattaa tattatgcac agtatgatta
      661 aatgctttcc cgttcacttc ttccttacca tgctcttgat accaagcttg tgaattatta
      721 acaaactctt tttgctgttc attcgtaatt ttggttttgt agttaattga aaaactatta
      781 agtgacccat agccttgtgg aattgttaca tcaattgtgt tcttcgtatt atcaacagtt
      841 attttagaac ctggaaaggc tttttcaaaa tcagtaattg tatttggtcc actataataa
      901 ttgctatgtg tacctgtcac attaatgttt aatgtgctta aatctaactg ctgtccacct
      961 tgaatctgat cctttatagt aatatctttc gatacatatc ttttttcatt attaatattt
     1021 aaaaaccatc gtacatgtgt cgtatcttct ggcagcatat ctcccgtttt ataatagaaa
     1081 acactacttg ttcccgcttc acttttatga accgtaacat tcgttgattt attcccagat
     1141 gttatcgtag ctactttgtc atctgaagta tttgtttgcg ttaaatttct tccttgtact
     1201 tcaaattctg caaatcccga aacatcactt aatttttcta ctttatcatt gaatgtaatt
     1261 gttgctccgt ctggcgtaat aactgcttga cccacctgtt cacctttaac agttaatgat
     1321 actgttttgc tataaccctc tatctttact gtaccacttg tcggccatgc cactttaatc
     1381 gtgtcaccat tttgtatttt tccattttta tcgtcgaacg tcatttttac tgtcgtttta
     1441 ccaccatctt ctatcttaga cggtgataca gttaaatctg taacattcgt tgatgaaata
     1501 tctcgtgctg caaatgcttc atttttatta aataaaggtg tgatgatatt taataacatt
     1561 ataaagacca taaacttcaa cacatttttg ttcataaaaa cccctcctta tactctatgt
     1621 catatccaca ggatactaat aataaattat aaactaattt agttcttatt caatgaaatt
     1681 tatcaaaaat tcatattatt attttattat gcaaaatata tacagtataa cgaatacaag
     1741 tgcatgaagt taacttgtat caattaattt cttagtatat tagttcattt tcaaaatctt
     1801 ttaaatttaa taaacaccaa ctataccagt tcttcactgc ttctcgaaaa ataattatat
     1861 taatgatttc gttaatttaa ttttatattt aattattact gtacatcttt tgtagttagc
     1921 tttattctta aattgaaata tgtataaaac atagaaagaa ggacgtttta acatggaaaa
     1981 cacggttaaa tatcgtaagt ttatactccc tatcgttgta ggtctcctaa tttgggcact
     2041 tacacctttt aaaccggata ctgtggatcc aacagcatgg tatatgttcg caatattcgt
     2101 cgcgacaatc attgcttgta ttacacaacc gatgccaatt ggggctgtct ctataattgg
     2161 atttacaatc atggtactcg ttggcattgt tgacatgaaa acggctgtcg ctggttttgg
     2221 taataatagc atttggttaa ttgctatggc ctttttcatt tcaagaggat ttgtgaaaac
     2281 aggtcttggt agacgtatcg cacttcattt cgtcaaatta tttggtaaaa aaacattagg
     2341 cttagcatat tctatcgtcg gtgtagattt aattctagcg cctgctacac caagtaatac
     2401 cgcgcgtgca ggtggaatca tgttcccaat tatcaaatca ctttctgaat catttggttc
     2461 gaaaccgaaa gacgaatcat cacgtaaaat gggtgcattt ctagttttca cagaattcca
     2521 aggtaactta attactgcgg ctatgttttt aactgcaatg gccggtaatc cccttgcaca
     2581 aaatttagca tctagcacat ctaatgttca cattacatgg atgaattggt tcttagctgc
     2641 tttagttcct ggacttgttt ccttaattgt tgtacctttt atcatttata aaatttatcc
     2701 accaactgtt aaagaaacac caaatgctaa aagttgggct gaaaatgaat tagcgactat
     2761 gggtaaaatc gctttagctg aaaaatttat gattggt
//
LOCUS       DAMZPP010000205         2764 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMZPP010000205 DAMZPP010000000
VERSION     DAMZPP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.207"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            1..128
                     /locus_tag="POH73_002678"
                     /pseudo
     CDS             1..128
                     /locus_tag="POH73_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(339..1139)
                     /locus_tag="POH73_002679"
     CDS             complement(339..1139)
                     /locus_tag="POH73_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6888577.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKLRFEANFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYKGNELYNYKSFNRQVFQQVVDYLNMNQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGHDSIDIIMPNMTKGQALKRLLDKWGMSSSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK"
     gene            complement(1369..1989)
                     /gene="srtA"
                     /locus_tag="POH73_002680"
     CDS             complement(1369..1989)
                     /gene="srtA"
                     /locus_tag="POH73_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HDI6888578.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDNKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVEVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            2183..2674
                     /locus_tag="POH73_002681"
     CDS             2183..2674
                     /locus_tag="POH73_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6888579.1"
                     /translation="MIRCAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNQWLDLAFYELD
                     LQD"
BASE COUNT          856 a          508 c          389 g         1011 t
ORIGIN      
        1 cagtttcata gtcaatgttt aaagcaaaac cttattgatg ataattcgat ttttattgat
       61 gtacaaaagt agaagctaat gccaataggt atacatttgc gtggaagaaa agtattcaaa
      121 atcatgaatt gttttatttt gtatatctgt atcaaaatcg atttgattaa aatccgcttt
      181 attttatcat ctattcaaat gattttagtg cgcttatttt actatggcat tattgcctaa
      241 cctgtttgag atatattaat atttgatgat tcgtattcaa agttttcatt taacatgtat
      301 ttagtatcat gatagctgct tcattgataa tctacttcct actttttcaa cacttcttgt
      361 tcgataatcg ttaaaacacc ctgcttatca ttacttggag cgacagcaga ggcaatattg
      421 aacaattctt catcatgact gttttccatc acataactat gcttagcgaa tgctagcata
      481 tctttatcat tattcgcatc tccgaaggcc atgagctctg aagaagacat tccccattta
      541 tctaacaatc gttttaatgc ctgcccctta gtcatgtttg gcataataat atcaatgcta
      601 tcgtgcccac ttgagacaag tttaatatca ttgctgaatt gcgttgctac ttcttcgtca
      661 acattcggat gcgtctcacg attaatatta aatgctattt tgacataatc atcctcaggt
      721 aattgttgta gactgtcaat ttcttttaac tgatgataat aaaatctcgt atcttcttta
      781 aatgcttcag aagtatgttt taaaatatac gcgcttttca aaccacagat gacgagttga
      841 tcaatacttt ggttcatatt taagtaatcg acaacctgtt gaaacacctg acgattaaag
      901 cttttataat tatataactc attgccttta taaataactg caccattttc agaaataaaa
      961 tacatatctc tatccccgaa aatagacttc aacttcgcat attgattacc actcgcagca
     1021 ataaatgtaa tatctctatt tctaagttca gtaaaattcg cttcaaatct aagtttatcg
     1081 tatgtctttt ttgaatctaa aaacgtcccg tccatatcta ccgcaatagc cttaaccatg
     1141 attatcctcc tcattgctct tttagtcgtc ttcattatat ccatctcatg acaagcaatg
     1201 aagtttgtca taccacaatg aacctatatt cacattttga aattgctttc atatatgcaa
     1261 aacacattac actggatatt aaattacttt ttacctcaca ccaaacaaaa aaacagtctc
     1321 tcaatcaaga ctgttttcta cttaatattc atccattatc acaatggatt atttgacttc
     1381 tgtagctaca aagattttac gtttttccca aacgcctgtc ttttcattgt aatcatcaca
     1441 agtaattaat gttaattgtt tatctttacc tttttgttca tctagaactt ctacatctgt
     1501 aggcttaaca tctcttatac ttgtcatttt atacttacgt gtttcattac caactttaaa
     1561 gtaaaccata ctaccttttt tggctgcttt aagatttgta aattgatagt tcggacggtc
     1621 aatgaaagtg tgtcctgcaa ttgaaatatt ttgatcatct agtgattcat tttcttctgc
     1681 aaagcttaca cctctattta attgttcagg tgttgctggt cctggatata ctggttcttt
     1741 aatatcagca tctggaattt caatatagcc tgccactttt gatttatctt tcggaatctg
     1801 aggtttagct tgctgcttat tgtctttact cgcctgttct tttacatttt tatcatattg
     1861 ttcaatcttt tcatctttat ctttatcgtg aagataatta tcgatatgtg gtttagcaaa
     1921 caaatatgct gccactagga taagtactac accagcgatt gtcattaatc gatttgtcca
     1981 ttttttcata cgttaaggct ccttttatac atttcacaat gcctattaac atttcatata
     2041 gtataccatt aattataaaa tgactcatag caattcattt tataatatta aatttgcaca
     2101 tatattgtca cgttagattt cattacacat atttgcattc aaataacgaa acgcttttaa
     2161 taattactaa gggggaattg ttatgattag atgcgctaaa aaagaggatt taaacgctat
     2221 attagcgata tacaatgatg ccattatcaa tactacagct gtttatactt atgaaccaca
     2281 aaccatagac gaacgtgtcg catggtttga aacgaaacaa cgtaagcatg agcctatctt
     2341 tgtatttgag gaaaatggaa gtgtcttagg gtttgcgacg ttcggttcgt ttagaccttg
     2401 gccagcatac ctatatacaa tcgaacattc tatttatgtc gatgcttcag ctagaggaaa
     2461 aggtattgct agtcaattgc tacaccattt aattgtggaa gcaaaagcta aaggttatcg
     2521 tgcactagtt gcaggcattg atgcttccaa caaagcgagt attcagttgc atcaaaagtt
     2581 tgcttttaag catgccggca cactgaccaa tgtaggtttt aaatttaatc aatggttaga
     2641 tttagcattt tacgaattag atttacaaga ctagtaatat ttgaatcaca taatataaac
     2701 aagacaacca tgttaattca cttaacataa caagccaaca tataaaattt taaacttctc
     2761 aggg
//
LOCUS       DAMZPP010000206         2734 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMZPP010000206 DAMZPP010000000
VERSION     DAMZPP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.208"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            123..1613
                     /locus_tag="POH73_002682"
     CDS             123..1613
                     /locus_tag="POH73_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDI6888580.1"
                     /translation="MDDKQHTTSSDDERAENATSNQDQQTNSSKRVHLKRWQFISILI
                     GTIIITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQNSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDQALENLV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            2014..2523
                     /locus_tag="POH73_002683"
     CDS             2014..2523
                     /locus_tag="POH73_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6888581.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDLVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            2535..>2734
                     /locus_tag="POH73_002684"
     CDS             2535..>2734
                     /locus_tag="POH73_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI6888582.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEVLYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRY"
BASE COUNT         1076 a          348 c          481 g          829 t
ORIGIN      
        1 taaaataaac taagttgtga ctaaaaatta atcaattata gtgaaatatg gtgcgctatc
       61 ttgcataaat tgatatgatt aactacacag aatttaaaag tacataataa ggaagtgata
      121 caatggatga taagcaacac acaacttcat ccgatgatga acgcgctgaa aatgcaacaa
      181 gcaatcaaga ccaacaaact aattcatcga aacgcgttca cttaaaacgt tggcaattca
      241 tatcaatttt aattggtaca atcataataa cagctgtcat cacagttgtt gcatatattt
      301 ttataaatca aaaaataagt ggtttaaaca aaactgatca agcaaactta aataaaattg
      361 aaaatgtgta taaaatctta aatagtgatt attataaaaa acagaactct gacaagttga
      421 gtaaagctgc aattgatggc atggtcaaag aattaaaaga tccttattct gaatatttaa
      481 caaaagaaca aactaaatcc tttaatgaag gtgtttcagg tgattttgta ggtattggtg
      541 cagaaatgca aaagaaaaat gatcaaatta tggttactag ccctatgaag ggttctccag
      601 cagaacgtgc tggcattcgt cctaaagatg tcattactaa agtaaatgga aaatcaatta
      661 aaggaaaagc attagacgaa gttgtcaaag acgttcgcgg taaagaaaac actgaagtaa
      721 ctttaactgt tcaaagaggc agtgaagaaa aagacgttaa gattaaacgt gaaaaaattc
      781 atgttaaaag cgttgagtat aagaaaaaag gtaaggttgg agttattact attaataaat
      841 tccagaatga tacatcaggt gaattgaaag atgcagttct taaagctcac aaagatggtt
      901 tgaaaaagat tgttttagat ttaagaaata atccaggtgg actactagat gaagctgtta
      961 aaatggcaaa tatttttatc gataaaggaa aaactgttgt taaactagaa aaaggtaaag
     1021 atactgaagc aattcaaact tctaatgatg cgttaaaaga agcgaaagac atggatatat
     1081 ccatcttagt gaatgaaggt tcagctagtg cttctgaagt gtttactggt gcgctaaagg
     1141 actataataa agctaaagtt tatgggtcaa aaacattcgg taaaggtgtc gtacaaacta
     1201 caagagagtt taaagatggt tcgttgttaa aatatactga aatgaaatgg ttaacgccag
     1261 atggtcatta tattcacggt aaaggcatta aaccagacgt tactattgac acacctaaat
     1321 atcaatcttt aaatgtcatt cctaatacga aaacatttaa agttggagac gatgataaaa
     1381 atattaaaac tattaaaatt ggtttatcag ctttaggtta taaagttgat aatgaatcaa
     1441 cgcaatttga tcaagcttta gaaaatctag ttaaagcttt ccaacaagcg aataaactcg
     1501 aggtaactgg tgaatttaat aaagaaacga ataataaatt tactgagtta ttagttgaaa
     1561 aagctaataa acatgatgat gttctcgata aattgattaa tattttaaaa taaagcgata
     1621 cacactacta aaattttatt attattatgt taatgacacg cttcctaaat ttgcaaagat
     1681 agcaatttag gaggcgtatt tatttttatt gacgtctaac tctaaaagat ataaattaga
     1741 catttacaaa tgatgtaaat aacacaattt ctatcatcgc tgttaacaat tcatgcttta
     1801 atatactatg agcatatact ttttaaatag tattattcac tagttttaac aatcaattaa
     1861 ttggtatatg atacttttat tggttatttt tatcccatag tgtgataatt actatttttc
     1921 attcataata aaggtttaaa gcatgctaat agtgtgtaag attaacatgt actgaaaaac
     1981 atgtttaaca taacgatata aggagtgacg tacatgatcc gtctaggtaa aatgtcagat
     2041 ttagatcaaa tcttaaatct agtagaagaa gcaaaagaat taatgaaaga acacgacaac
     2101 gagcaatggg acgatcagta ccctctttta gaacattttg aagaagatat tgctaaagat
     2161 tatttgtacg tattagagga aaatgacaaa atttatggct ttattgttgt cgaccaagac
     2221 caagcagaat ggtatgatga cattgactgg ccagtaaata gagaaggcgc ttttgttatt
     2281 catcgattaa ctggttcgaa agaatataaa ggagctgcta cagaattatt caattatgtt
     2341 attgatttag ttaaagcacg tggtgcagaa gttattttaa cggacacctt tgcgttaaac
     2401 aaacctgcac aaggtttatt tgccaaattt ggatttcata aggtcggtga acaattaatg
     2461 gaatatccgc catatgataa aggtgaacca ttttatgcgt attataaaaa tttaaaagaa
     2521 tagaggtaat attaatgacg aaaatcgcat ttaccggagg gggaacagtt ggacacgtat
     2581 cagtaaattt aagtttaatt ccaactgcat tatcacaagg ttatgaagtg ctttatattg
     2641 gttctaaaaa tggtattgaa agagaaatga ttgaatcaca actaccagaa attaagtatt
     2701 atcctatttc gagtggtaaa ttaagaagat atat
//
LOCUS       DAMZPP010000207         2720 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMZPP010000207 DAMZPP010000000
VERSION     DAMZPP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.209"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(1..689)
                     /locus_tag="POH73_002685"
                     /pseudo
     CDS             complement(1..689)
                     /locus_tag="POH73_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001802535.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            complement(796..1569)
                     /locus_tag="POH73_002686"
     CDS             complement(796..1569)
                     /locus_tag="POH73_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6888583.1"
                     /translation="MIHSKRLKMCLCLIILSVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDEEFDKGDKGTWIVDSEMVVELKDKKMESRSMVLYINRNT
                     RTTKGNFIVRELWEDSKGYAQSKDTKYPVKMEHNRIIPTKQIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAEYQLSNNDYNVKQLRKRYNIPTKKAPKLLIK
                     GDGDLKGSSIGHKNLEFTFVENKEENIYFTDSINFKPTE"
     gene            complement(1876..>2720)
                     /locus_tag="POH73_002687"
     CDS             complement(1876..>2720)
                     /locus_tag="POH73_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HDI6888584.1"
                     /translation="LLTENESKNLVFFSRQIAPGLKRIDSIVLEELLKNELTYDELKN
                     KMLNEVKDITEDDIDTSLRILDFSFYNAGIEKIYGSPIIECNERMIRLSDAFTNALSN
                     QTFKIFLEDLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWSKNGSSVIMGYMI
                     RSQEMPIFITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLKSKEVQKILSHRAKG
                     IKMYIFVQKKDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYR
                     YITN"
BASE COUNT          811 a          464 c          283 g         1162 t
ORIGIN      
        1 taactcttta gaccctacag atgagccttt taaatcacca tcgcctttta ataacatttt
       61 cggtgcttgt ttcgttggta tgtcatatct ttttcttaac tgttgcacat tataatcatc
      121 attcctcaat tgatattttt cggaataact tggcacatta ggattatatg aaatatcacc
      181 atttttataa tcattaatat ctttaaaatt gccatattgt acaaaaaatt taaagttttc
      241 aatttctttt ttcaacttgt catcttgtat tggcttcgtt gggataattt gattatgttc
      301 cattttgact ggatattttt ttctttttca tacgtttttc cttttgtttc acttacaaaa
      361 aaataacctg ttgtcctttt tgtatttcta ttcatatata gaatcattcc tcttgatttt
      421 aaactttctc cctttaattg aatattcatt tcagagctga ttatccatgt acctttgtct
      481 cctttgtcaa attcttgatc tcgaaagcct tctttatcgt aaaaatcatc gagatttttg
      541 gttggatata attttaatgt ttgattaaat gtgtctttaa tttgggcgct tcgactattt
      601 atttttgtca taatgacata acctacaatc aatataatga gtaataccaa acatataccg
      661 agcgttaatt tctttgaatg aatcatcatt tctccacctt atatttttaa tactgactca
      721 gtttttaata tttacaacta aagaaaaatt attttgaaca ctttatggaa ttttcattcc
      781 aaacttctat taaccctatt cagtaggttt gaaattaata ctatccgtaa aatagatgtt
      841 ttcttctttg ttttctacaa aggtaaactc taaatttttg tgtcctatag atgatccttt
      901 taaatcacca tcacctttta ttaataattt aggtgctttc ttagttggta tattatacct
      961 ctttctaagt tgcttcacat tgtagtcatt gttactcaat tgatattctg ctgaataact
     1021 aggtacattt ggattatatg aaatgtcacc atctttataa tcattaatat ctttaaagtc
     1081 accgtattgt acaaaaaatt taaagttttc gatttctttt cttaatttat catcagctat
     1141 ctgtttggtt gggataattc gattatgttc cattttcact ggatattttg tatctttact
     1201 ttgtgcatac cctttactat cttcccacaa ttctctaaca ataaaattac ctttcgttgt
     1261 tctagtatta cgatttatat agagcaccat acttctagat tccatttttt tatccttcag
     1321 ctcaactacc atctcagaat caacaatcca agtgccttta tctcctttat caaactcttc
     1381 gtcacgaaat ccttccttat cataaaagtc ttcaagattt ttagttggat acaggctcaa
     1441 cgttttgttg aagttttctt taatttgttt atctttacta ctttcttctt ttttcattcc
     1501 gcaagcccct ataaaaacag atagtattat caagcataga cacattttca acctttttga
     1561 atgaatcatc atttcacatc cccattttta tttttgatgc aaagttaagt tcaaattcca
     1621 atattaataa tactagagga aattttatga ttaaatcctc gtggctttta attgttagtt
     1681 gtatttttca catattaaca tcacaaatta tttaaaaaag cgcaaatatc tttataattt
     1741 ttattggcct aaccaactaa ttaattaaga taaattgcgc ttatatttaa aataataaca
     1801 ctgaaactca atgtctttac ttgttaaaat tgatgtttaa atacatcttt actaacattc
     1861 atttttcggt ttacattaat ttgttatata gcgatatata tcatctctga cagctttatc
     1921 gagtgctaaa tccatagtta ctacgtttga accattcggc attttatctt gcttctctga
     1981 accttctata tatccagcag ttcctaaata ataaaaatat ataccatcat catctttttt
     2041 ctgtacaaaa atatacattt taattccttt tgctctatgg gacaaaattt tctgaacttc
     2101 ttttgatttt agcgttctat tagatttagt aaaccatttt aattcatctt gacttaaaaa
     2161 ttcatcttca tattttgtgc tatcacttat atcctcatgc ttatcataag tgataaatat
     2221 tggcatttct tggcttctta tcatgtatcc catgattaca gacgaaccat ttttactcca
     2281 attaaatatt ttactaaaat cttctcttga atatttatta tataaaatca gtccattttt
     2341 acctttttgg tatttttcat tgttatactt tgataattca ataagatctt ctaaaaatat
     2401 tttaaatgtt tgattactta gagcattagt aaatgcatcg cttagtctta tcattctttc
     2461 attacattct ataatgggac ttccatagat tttttcaatt cctgcgttgt aaaatgaaaa
     2521 atctaaaatt cttaatgaag tatctatatc atcttctgtt atatccttaa cttcgttcaa
     2581 cattttattt tttaattcat catatgttaa ttcatttttt aaaagttctt ctaaaactat
     2641 actatcgatt cttttcaatc caggtgctat ttgtcttgaa aagaaaacca gatttttaga
     2701 ttcattttcc gtcaataaag
//
LOCUS       DAMZPP010000208         2679 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMZPP010000208 DAMZPP010000000
VERSION     DAMZPP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.210"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            42..326
                     /locus_tag="POH73_002688"
     CDS             42..326
                     /locus_tag="POH73_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDI6888585.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            310..672
                     /locus_tag="POH73_002689"
     CDS             310..672
                     /locus_tag="POH73_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HDI6888586.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            669..1073
                     /locus_tag="POH73_002690"
     CDS             669..1073
                     /locus_tag="POH73_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDI6888587.1"
                     /translation="MSVKVIGDKALERELEKRFGIKEMVKVQDKALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVYWRGSKDRYKIVHLIEYGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            1070..1477
                     /locus_tag="POH73_002691"
     CDS             1070..1477
                     /locus_tag="POH73_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888588.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDECAYSYIVQIDVFVKYNDEYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSSRVYEGIFYKEEN"
     gene            1478..2122
                     /locus_tag="POH73_002692"
     CDS             1478..2122
                     /locus_tag="POH73_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6888589.1"
                     /translation="MAVKHASAPKAYINITGLGFAKLTKEGAELKYSDITKTKGLQKI
                     GVETGGELKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEDYDEDGVYEEK
                     QGKQNNYVAVWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLVDNKKSVRKYIFDSANMTNHDGDGEKGEEAFLKKILGEEYTGNVTEDSEETL"
     gene            2164..2388
                     /locus_tag="POH73_002693"
     CDS             2164..2388
                     /locus_tag="POH73_002693"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDI6888590.1"
                     /translation="MPDSIKTLKVGDTYDLNVVVEPSNQSKLLKYTTDQTNIVSINSD
                     GQVTAEAQGIATVKATVGNMSDTITINVEA"
     gene            2438..>2679
                     /locus_tag="POH73_002694"
     CDS             2438..>2679
                     /locus_tag="POH73_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888591.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFTVKDLK"
BASE COUNT         1052 a          340 c          548 g          739 t
ORIGIN      
        1 ggttaaaagt gaaatcgtcg acttattgaa tggtgaagac aatgacgatt gatgatttgc
       61 ttgtcaaatt taaatcactt gaaaagattg accataattc agaggatgag tacttaaagc
      121 agttgttaaa aatgtcgtac gagcgtataa aaaatcagtg cggagttttt gaattagaga
      181 atttaatagg tcaagaattg atacttatac gcgctagata tgcttatcaa gatttattag
      241 aacacttcaa cgataattac agacctgaaa taatagattt ttcgttatct ctaatggagg
      301 tatcagaaga tgaagaaagt gtttaaaaaa cctagaatta caactaaacg tttaaatact
      361 cgtgttcatt tttataagta tactgaaaat aatggtccag aagctggaga aaaagaagaa
      421 aaattattat atagctgttg ggcgagtatt gatggtgtct ggttacgtga attagaacaa
      481 gctatctcaa acggaaccca aaatgacatt aaattgtata ttcgtgatcc gcaaggtgat
      541 tatttaccca gtgaagaaca ttatcttgaa attgaatcaa gatatttcaa aaatcgtttg
      601 aatataaagc aagtatcacc agatttggat aataaagact ttattatgat tcgtggagga
      661 tatagttcat gagtgtgaaa gtgataggtg ataaagcatt agaaagagaa ttagaaaaac
      721 gttttggcat aaaagagatg gtaaaagttc aagataaggc gctaatagct ggtgctaagg
      781 taattgttga agaagtaaaa aaacaactaa agccctcaaa agatacggga gcattaatta
      841 atgaggtaag ttttagtaaa cctgaatgga taaacggaaa acgtacaatt actgtttatt
      901 ggcgaggttc taaagaccgt tataaaatcg tacatttaat tgaatatgga cacgttcaaa
      961 aaggaacagg taaatttatc aaacctaaag ctatgggcgg tgttaataga gcaataagac
     1021 aagggcaaaa taagtatttt gagacgctaa aaagggagtt gaaaaaattg tgattgatat
     1081 tttgtacaaa gttcatgaag tgattagtca agacagaatt attagagagc acgtaaatat
     1141 caataatatt aagttcaata aataccctaa tgtaaaagat actgatgtac cttttattgt
     1201 tattgacgat atcgacgacc caatacctac aacttatact gacggagatg agtgtgcata
     1261 tagttatatt gtccaaatag atgtttttgt taagtacaat gatgaatata atgcgagaat
     1321 cataagaaat aagatatcta atcgcattca aaagttatta tggtctgaac taaaaatggg
     1381 aaatgtttca aatggaaaac cggaatatat agaagaattt aaaacatata gaagctctcg
     1441 cgtttacgag ggcatttttt ataaggagga aaattaaatg gcagtaaaac atgcaagtgc
     1501 gccaaaggcg tatattaaca ttactggttt aggtttcgct aaattaacga aagaaggcgc
     1561 ggaattaaaa tatagtgata ttacaaaaac aaaaggatta caaaaaattg gtgttgaaac
     1621 tggtggagaa cttaaaacag cttatgctga tggcggtcca attgaatcag ggaatacaga
     1681 cggagaaggt aaaatctcat tacaaatgca tgcgttccct aaagagattc gcaaaattgt
     1741 ttttaatgaa gattatgatg aagatggcgt ttacgaagag aaacaaggta aacaaaacaa
     1801 ttacgtagct gtatggttca gacaagagcg tagagacggt acatttagaa cagttttatt
     1861 acctaaagtt atgtttacaa atcctaaaat cgatggagaa acggctgaga aagattggga
     1921 tttctcaagt gaagaggttg aaggtgaggc acttttccct ttagttgata ataaaaagtc
     1981 agtacgtaag tatatctttg attcagctaa catgacaaat catgatggag acggtgaaaa
     2041 aggcgaagag gctttcttaa agaaaatttt aggcgaagaa tatactggaa acgtgacaga
     2101 ggatagcgaa gaaactttgt aacgaaaccg gcttcatcgg aaactgcggt aaagtcggtt
     2161 aatataccag atagcattaa aacacttaaa gttggcgaca catacgattt aaatgttgta
     2221 gtagagccat ctaatcaaag taagttattg aaatacacaa cagatcaaac gaatattgta
     2281 tcaatcaata gtgatggtca agttactgcg gaagcacaag gcattgctac ggttaaagca
     2341 acagttggta atatgagtga cactataaca ataaatgtag aagcataaga gggggcaacc
     2401 cctctatttt atttgaaaat aaggagagta ttataaaatg gcaaaattaa aacgtaacat
     2461 tattcaatta gtagaagacc cgaaagcaaa tgaaattaaa ttacaaacgt acttaacacc
     2521 acacttcatt tcatttgaaa ttgtatacga agcaatggat ttaatcgatg atattgagga
     2581 cgaaaatagc acgatgaaac caagagaaat cgctgacaga ttgatggata tggttgtaaa
     2641 aatttacgat aaccaattca cagttaaaga cctaaaaga
//
LOCUS       DAMZPP010000209         2608 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMZPP010000209 DAMZPP010000000
VERSION     DAMZPP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.211"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..1982
                     /locus_tag="POH73_002695"
     CDS             <1..1982
                     /locus_tag="POH73_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HDI6888592.1"
                     /translation="AKNAIKALANAKRDQINSNPDLTLEQKAKALKEIDEAEKRALEN
                     IENAQTKDQLNQGLNLGLDDIRNTHVWEVDAQPAVNEIFDATPEQILVNGELIVHRDD
                     IITEQDILAHINLIDQLTAEIIDTPSTATISDSLTAKVEVTLLDGSKVIVNVPVKVVE
                     KELTVVKQQAIESIENAAQQKINEINNHATLTPEQKEAAIAEVNKLKQQAIEQINNAA
                     DVHTVEEVQHQEQAHIEQFNPDQFTIDQAKSNAIKSISDAIQHMIDEINASKDLTDKE
                     KQEAISKLNQLKDQSIQAIQRAQSIDEIAQQLEQFKAQLKAANPFAKELENRKKAAIS
                     KIKDISTDKIDRIRNSTIGTAEERQAAMNRINEIVLETIKDINNAQTPQQVEAALNNG
                     IARILAVQIVTSDHSKPSSNSDGQSNSHLHVGYGTVNHPFNSSPIGHKKKLDQDDEID
                     PLHMRHFGDRIGNVIKNALGVVGISGLLASFWFFIAKRRRKEDEEEELEIRDNSKDKK
                     KGSIEGTKHLPLLFAKRRRKEDEEDAIVEEKDSLNNDESLDKVKHTPFFLPKRRRKED
                     EEDVEVTNENTDEKVLQDNEHSPVLIAKRLKDKDGNVETTTSIESKDEDVPLLLAKKK
                     NQKDNQSKGKKSASKKPSKKVAAKKKKKKSKKNKK"
     gene            complement(2041..2442)
                     /locus_tag="POH73_002696"
     CDS             complement(2041..2442)
                     /locus_tag="POH73_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDI6888593.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVSNALLYTDSKLIADSVNAGYVKNAKFKPYFDQLEIFEQDFD
                     LLFVKWIPREQNKEANQHAQQALYKFIKKNK"
BASE COUNT         1018 a          435 c          445 g          710 t
ORIGIN      
        1 atgcgaaaaa tgcaattaaa gcattagcaa atgcgaagcg tgatcaaatc aattcaaacc
       61 ctgatttaac acttgaacaa aaagcaaaag cgctcaaaga aattgacgaa gctgaaaaac
      121 gagcactaga aaacattgaa aatgctcaaa ctaaagatca attaaatcaa ggattaaatt
      181 taggattaga tgatattaga aatacacatg tatgggaagt tgatgcacaa cctgctgtaa
      241 atgaaatttt tgacgcaact cctgaacaaa tccttgtaaa tggcgaactc attgtccatc
      301 gtgatgacat cattacagaa caagatattc ttgcacacat caacttaatt gatcaactta
      361 cagcggaaat tattgataca ccgtcaactg caacaatttc tgatagttta acagcaaaag
      421 ttgaagtaac attgcttgat ggatcaaaag tgattgttaa tgttcctgtg aaagtagtag
      481 aaaaagaatt gacagtagtt aaacagcagg cgattgaatc aattgaaaat gcggcacaac
      541 aaaagattaa tgaaatcaat aatcacgcaa cattaacacc tgaacaaaaa gaagctgcga
      601 ttgcggaagt taataagctt aaacaacaag ctattgagca aattaacaac gcagcagatg
      661 ttcatacggt ggaagaagtt caacaccaag aacaggcgca tattgaacaa ttcaatccag
      721 atcaatttac aattgaccaa gccaaatcaa atgccattaa atcaatttct gatgctatcc
      781 aacatatgat tgatgaaatt aatgctagta aggatttaac tgataaagag aagcaagaag
      841 ctatatctaa attaaatcag ttaaaagatc aatcaattca ggcgattcaa cgtgcgcaaa
      901 gcatcgatga aattgcgcaa caattagagc aatttaaagc gcaactaaaa gcggccaatc
      961 catttgctaa agaattggaa aaccgtaaaa aagctgcgat ttcaaaaatt aaagatattt
     1021 ctacagataa aattgatcgc attagaaata gcacaattgg aactgctgaa gaaagacaag
     1081 cagcaatgaa tcgaattaac gaaattgtgc ttgaaacaat caaagatatt aataatgcgc
     1141 aaacgccaca acaagttgag gctgcattga ataatggtat cgcacgaatt ttagcagtac
     1201 aaattgtaac atcagatcat agcaaacctt catcaaattc agatggtcaa tctaatagtc
     1261 atttacacgt tggttatggc actgtaaatc atccattcaa tagttcgcct attggacata
     1321 agaagaaatt ggatcaagat gatgaaatcg atccacttca tatgcgtcat ttcggtgacc
     1381 gaattggtaa tgttatcaaa aacgcacttg gcgttgttgg tatttctgga ttacttgcta
     1441 gcttctggtt cttcattgcg aaacgtcgtc gtaaagagga tgaagaggaa gaattggaaa
     1501 ttagagataa tagcaaagac aagaagaaag gttcaataga aggtactaaa catttaccac
     1561 ttttatttgc gaaacgtcgt agaaaagaag atgaagaaga tgctattgtt gaagaaaaag
     1621 attcgctaaa caatgacgag tcactcgata aagttaaaca tacgccgttc ttcttaccta
     1681 aacgtcgcag aaaagaagat gaagaagatg tggaagttac aaatgaaaac acagatgaaa
     1741 aagtgttgca agataacgaa cattcaccgg tcttaatcgc aaaacgactc aaagataaag
     1801 atggaaatgt tgaaacaaca actagtattg aatctaaaga tgaagacgtt cctttattat
     1861 tggctaaaaa gaaaaatcaa aaagataacc aatccaaagg gaaaaagtcg gcatcaaaaa
     1921 aaccttctaa aaaggtagca gctaaaaaga agaaaaagaa atctaaaaaa aataaaaaat
     1981 aatttgtttc ttcgataaaa agaggagcac cgattgaaaa tatatcagtc ggtgctcctt
     2041 ttatttgttc tttttaataa atttgtacaa tgcttgctgt gcatgttgat tcgcttcttt
     2101 gttttgttct ctcggaatcc atttaacaaa taataaatca aaatcttgtt caaatatttc
     2161 caattgatca aaataaggtt tgaatttggc attttttaca taaccagcgt taacactatc
     2221 cgcaattaac ttagagtcag tgtacaacaa cgcattacta acatttaatt cacgtgcatg
     2281 ttctagtgca taaatacatg ctgcccattc tgctgtgtga ttatccattt cacctaactc
     2341 atgtgtatat gtataatgct gctcatcttc tttgattaca atggcacaag tacttatgcc
     2401 tggatttcct ttcgtcgcag catcaaaatt tattttcgcc ataataaacc tactttctat
     2461 tcaatactta gttaaagtta ctattactgt aatacaaaat acgttgggta atccataaaa
     2521 aaacacgcat cacttaaata agtaacacgt gtttaaaata ctcgctgagt caaagatgat
     2581 tttctaatac gtagtactgt aatatact
//
LOCUS       DAMZPP010000210         2600 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMZPP010000210 DAMZPP010000000
VERSION     DAMZPP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.212"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..1362
                     /gene="recN"
                     /locus_tag="POH73_002697"
     CDS             <1..1362
                     /gene="recN"
                     /locus_tag="POH73_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDI6888594.1"
                     /translation="KINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNY
                     AESRYQDLLDKYHQTFQNYKAKKQGLEDLESADQALLQRLDLMKFQLEELSEAHLKEG
                     EIEQLEIDIKRIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYD
                     KLKEDIDQFYYILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISEL
                     IAYQEKLNNEINKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIV
                     SEIQNLQMKDANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRI
                     MLALKSIFVKSRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVAS
                     MSDHHLLISKSSKDDRTTTQVQELIGDDKVDEIARMISGASVTDLTRENAREMIQHNQ
                     RHR"
     gene            1513..>2600
                     /locus_tag="POH73_002698"
     CDS             1513..>2600
                     /locus_tag="POH73_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI6888595.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSVGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIA"
BASE COUNT          999 a          361 c          469 g          771 t
ORIGIN      
        1 aaaattaata atcaaacagt tactttacaa gatttacgaa aagttatgca agaattatta
       61 gatattcatg gccaacatga aacgcaatct ttgttgaaac aaaaatacca tttaacatta
      121 ttggataatt acgcagaatc acgttatcaa gatttgttgg ataaatatca ccaaactttt
      181 caaaattata aagccaaaaa gcaagggtta gaagatttag aatcagcaga ccaagcattg
      241 ctgcagcgtt tagatttaat gaaattccaa ttagaagaat tgtctgaagc acatctaaaa
      301 gaaggcgaaa tagagcaact tgaaatagat attaaacgca ttcaaaactc tgaaaaatta
      361 agcttagctc ttaataacgc acatatgaca ttaacggatg aaaatgctat cactgatcgt
      421 ttgtatgaac taagcaacca tttgttaaca ataaacgata tagttccaaa caaatatgac
      481 aaattaaaag aagatattga tcaattttat tacattttag aagatgcaaa acatgagtta
      541 tatgatgaaa tggctaatac agaattcgac gaacaagtgt taaatgagta tgaatctcgt
      601 atgaatttgc tgaataattt aaaacgcaaa tatggaaaag atatttcaga attaatcgca
      661 tatcaagaaa aacttaataa tgaaatcaat aaaattgaaa actatgaaca aagtacatcg
      721 cagttacgag aagaaataaa tgcattgtat aatcaagtta tagaggttgg acaagcgtta
      781 tctaagcagc gtcgtatagt tgctagggag ttaagagacc acattgtatc ggaaattcaa
      841 aacttacaaa tgaaagatgc aaatcttgag atttcattta aaaaattaga agaaccgaat
      901 attgatggaa tcgaatttgt agaattttta atcagtccta ataaagggga accattaaaa
      961 agcttaaata aaattgcgtc aggtggagaa ctttctagaa ttatgttagc gttaaaaagt
     1021 atttttgtta aatcgagagg tcaaactgca attttattcg atgaggttga ctcaggtgta
     1081 tctggacaag ccgcacaaaa aatggcagag aaaatgcgag atatagcaga atatatacaa
     1141 gttatttgta tatctcactt gccacaagtt gcatcgatga gcgaccatca tttattaatt
     1201 tcgaaatcat caaaagatga tcgtacaaca acacaagttc aagagttaat tggcgatgat
     1261 aaagtagatg aaattgcacg aatgatttca ggtgcaagtg ttactgattt aactcgtgaa
     1321 aatgcaagag aaatgataca acacaatcaa agacatagat aatttccaaa gtgtgttgaa
     1381 tactcattta tggatttatt tatgtgcatt caaactaaat agacaatcat gtaaaatagg
     1441 taattaatgg acatgaaaac tatgtattaa taaattttca atggatatat atcattagtt
     1501 aggagtaaga acatgtcaga gaaacaatat gatttagtcg ttctcggtgg aggtactgca
     1561 ggatatgttg ctgcgatacg tgcgtctcaa ttaggtaaaa aagtggcaat agtagaacga
     1621 caactattag gaggtacttg tctccataaa ggttgtattc ctactaaatc gctgcttaaa
     1681 tctgctgaag tatttcaaac agtaaaacag gcagcaatgt ttggcgttga tgtcaaagat
     1741 gctaatgtta attttgaaaa tatgttggca cgaaaagaag acattattaa tcaaatgtat
     1801 caaggtgtaa agcatttaat gcaacacaat cacattgaca tttataatgg cacgggacgt
     1861 attctaggta catctatatt ttcacctcaa agtggcacaa tatctgtcga atatgaagat
     1921 ggtgaatcag acttattacc caaccaattt gtattaattg caactggttc ttcgcctgca
     1981 gagttgccat tcttatcatt tgatcatgat aaaattttat ctagcgatga tatattatca
     2041 ttaaagacgt taccatcaag tgttggtatt attggtggtg gtgttatcgg aatggaattt
     2101 gcatcattga tgatagattt aggtgttgac gtaacagtta tagaagctgg tgaaagaatt
     2161 ttaccaactg aaagtaaaca agcttcacaa ctattaaaaa aatcattgtc agcacgtggt
     2221 gttaaattct atgagggaat aaaactttct gaaaatgata tcaatgttaa cgaagatggt
     2281 gttacatttg aaatttcctc agacataatt aaagtagata aagtgttgct atcgattggt
     2341 agaaaaccta acacatcaga tattggttta aacaacacta aaataaaact ttcaacatca
     2401 ggacatattt taacgaacga atttcaacaa actgaagata aacatattta tgcagctggt
     2461 gattgtattg gaaaattaca attggcacat gttggatcaa aagaaggtgt tgtggctgtc
     2521 gatcatatgt ttgaagggaa cccaatccca gtaaactata acatgatgcc taagtgtatt
     2581 tattcacaac ctgaaattgc
//
LOCUS       DAMZPP010000211         2592 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMZPP010000211 DAMZPP010000000
VERSION     DAMZPP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.213"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..163)
                     /locus_tag="POH73_002699"
     CDS             complement(<1..163)
                     /locus_tag="POH73_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase"
                     /protein_id="HDI6888596.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIQDIKVHSRGTGSW
                     NLGEPPHEGT"
     gene            310..510
                     /locus_tag="POH73_002700"
     CDS             310..510
                     /locus_tag="POH73_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HDI6888597.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHD"
     gene            513..1769
                     /locus_tag="POH73_002701"
     CDS             513..1769
                     /locus_tag="POH73_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDI6888598.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYRCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAEKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            1859..2389
                     /locus_tag="POH73_002702"
     CDS             1859..2389
                     /locus_tag="POH73_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDI6888599.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
BASE COUNT          925 a          361 c          498 g          808 t
ORIGIN      
        1 gtgtaccttc atgaggcggc tctcctaaat tccagctacc agtacctctt gaatgtactt
       61 ttatatcttg aatatttctg tctttaagtc tttgtcgcat gattgcttct gccattggag
      121 aacgacatat attgccaaga cagacaaatg ctacatctac cattttgatt cctccaaact
      181 atgtagttat atccccattt tatagcgact ttaaacaata agaaagcaga ttatataaaa
      241 ttctattaaa gtttattaaa ttgtgatact ttgataacat aactattatt agaggtgaac
      301 attgtggcta tgacaaatga agagaaagta ttagctatta gagagaagtt aaatattgtt
      361 aatcaaggat tattagatcc tgaaaaatat aaaaatgcaa atgaagaaga attaacagat
      421 atatatgatt ttgttcaatc aagagaaaga ttgtcgccaa gtgaagtgac agctattgct
      481 gacgctttag gacaattgcg acacgactag gagtgggaat tttgactaat tacaaagaaa
      541 agttacaaca atacgctgaa ctattagtta aggttggtat gaatgttcaa ccaaaacagc
      601 cagtatttat tagatcatca gtagaaacat tagaattaac gcatttaatt gtagaagaag
      661 cttatcgttg tggtgcttca gatgtacgtg ttgtatatag cgatccaacc ttaaaacgct
      721 taaaatttga aaatgaatct gtggaacatt ttgctaatca tgagataaaa tcatatgatg
      781 ttgaagcgag aatggattac gttaaacgtg gcgctgctaa tttagcactt attagtgaag
      841 atccagattt aatggatggt atcgatagcc aaaaattaca agcatttcaa caacaaaacg
      901 cacgcgcatt taaaggatat atggaaagtg tacaaaaaaa tcaatttcca tgggtcgtag
      961 ctgcttttcc atctaaagct tgggcgaaga gagtatatcc tgaactatct gtagaagaag
     1021 catatattaa gtttattgac gaggtattcg atattgtgcg aattgatggt aacgatccag
     1081 ttgaaaattg gcgtcaacac atcgctaatt taagtgttta cgcacaaaaa ttacagcaaa
     1141 aaaattacca tgcgttgcat tatgtatctg aaggcactga tttaactgta ggtttagcga
     1201 aaaatcatat atgggaagat gctacaagtt atgttaacgg taaggaacaa gcttttattg
     1261 ctaatatacc aactgaagaa gtatttacag caccagatcg aaatagagta gatggatatg
     1321 ttactaataa attgccttta agttataatg gcacaattat cgatcaattt aaattgatgt
     1381 ttaaagatgg agaaattatt gatttttcag ctgaaaaagg tgaagcggta ttgaaagatt
     1441 taatcaatac agatgaaggt tcaagaagat taggtgaagt tgcgttagta cctgatgatt
     1501 caccgatatc aaatcgtaat accatttttt acaatacttt atttgatgaa aatgcagcat
     1561 gtcatttagc gattggatct gcttacgcct ttaatattca aggtggaacg gaaatgactg
     1621 ttgaggaaaa gattgcaagc ggattaaatg attcaaacgt acatgtcgac ttcatgattg
     1681 gtagtagtga tttgactatt tatggcatat tcgaagatgg ttcaaaagaa ctagtatttg
     1741 aaaatggaaa ttgggcatca acattttaat aaatgttatt ttgaggtgct gagtaggaaa
     1801 tgaaacatgt atttaaaggt caaatacgtg attaaagtat agattgggag ataaaataat
     1861 gacaaatcag gacagaccaa tgaaatctat gtcagaatca aaatgttata aaaatagaca
     1921 agttttccct caagatacga atcaccatca tacaatgttt ggtggtacat tgatggctaa
     1981 tattgatgaa attgcagcaa tcacagctat gaaacatgct ggtgcgcaag tagttaccgc
     2041 atctacagac tcagtagatt tcttaaagcc gattaaaaca ggggacatat tacaatacgt
     2101 agcgatggtt tcatacgctg ggacaagttc gatggaagtg gtcgttcaaa ttagaattga
     2161 cgatgtattt aataacaagc atgatttagc tgcattaagt tacttaacat ttgttgcgtt
     2221 agatgatgaa gggaagccta aacatgtacc tggagtatat ccagaagatg atgttgaaaa
     2281 atggttttat gatacagcac cacaacgtgt tgaacgaaga aaagcacgtc gcattgaaag
     2341 taaacaaaca attgaatatt tagctcaagt gcaacatatt agagactaaa aatataaaga
     2401 cagattgtta aggatgaatt gaactgttat gttcgaatat tccacctaca atctgtcttt
     2461 ttgattatgc aaatttatgt ttttagttaa aatttaaccc ttttattata gactattagt
     2521 cctattaaat gacctttatt atttaagtag agtgctgcca gcaattaatt gttttttatt
     2581 ttgtaatagc at
//
LOCUS       DAMZPP010000212         2591 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMZPP010000212 DAMZPP010000000
VERSION     DAMZPP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.214"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(182..397)
                     /locus_tag="POH73_002703"
     CDS             complement(182..397)
                     /locus_tag="POH73_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888600.1"
                     /translation="MIYSPSHYDDMFILNTQAHARLDQMAQQFEVVCNGLNENEGQAI
                     QTMDQSASLIRSNLIQVKEQLEKLAVY"
     gene            complement(576..1586)
                     /locus_tag="POH73_002704"
     CDS             complement(576..1586)
                     /locus_tag="POH73_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI6888601.1"
                     /translation="MKLSDREWKAFTTDELFSIKSGTTSDFSKLNLKNDSKLPCIGAK
                     YKNNGVVGFANNEELKVEGNALVFIKTGEGSVGLTLYKAEDFIPHKNVYIGYNVSLNK
                     YIGLFIATMYNKQKFIYNYGYGLNQLRVKRRKILLPSKNNSPDYEFMERYIKEKYFNL
                     KSHIKEKQKHKISDWRELDEVEWLPFSTSEVFNTIQRGKRLTKSNQIKGLTPYISSSG
                     INNGLDNFISNSEKIRVFDNCLTIANSGSVGSTFYHTYRFIASDHVTHLKNNNLNKFT
                     YLFIANMLRRLEGKYSFNREINDFRIKRERILLPTKNNQPDYEFMEQYMKRKENKILD
                     RL"
     gene            complement(1573..>2591)
                     /locus_tag="POH73_002705"
     CDS             complement(1573..>2591)
                     /locus_tag="POH73_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDI6888602.1"
                     /translation="NIKHSDMDILGNFYGEFVKYGGNDGNSLGIVLTPRHITNLMCEL
                     IDIDENDYVLDPCCGSGGFLIAAMNRMLGKTNDEIKKAEIKQNQLHGIELQQKLFTIA
                     TTNMILRGDGKSNLKRDDIFHVETGLYKNQITKALINPPYSQAKTKNLSHLSEISFIN
                     ETLSLMKKDAKLAAIVPQSTMIGKTNNDKNYKREILEKHSLETVITLNKDTFYGVGVN
                     PCIAIFTAGVPQDNKKRVNFVNFSDDGYIVRKHVGLVGDGTEKSKKEYLLNVLNDYED
                     ADTNFLVKSPITWKDEWLHSFFYYNEEIPTDEDFEKTIADYLSFEFDMKLHGRGDLFD
                     NETE"
BASE COUNT          789 a          444 c          350 g         1008 t
ORIGIN      
        1 gattttgttt ctttattaga ttcgttattg gtagttgaac atcctgatag cagtagcgtt
       61 gctattaaga ttaattttgc ctttttaagc atatttcatc acctatttat gtgtttgtaa
      121 aacttttatg tttgaaaaag ctacttattc tcaatgaaaa caagtagctt ttaataaata
      181 attagtatac tgctagtttt tctaattgtt ctttaacttg aattaagttt gaacgaatta
      241 acgaagcaga ttgatccatt gtttgaatag cttgcccttc attttcgttc aagccattac
      301 aaacaacttc aaactgttgt gccatttgat caagacgtgc atgagcttga gtgtttaaaa
      361 taaacatatc atcataatga gatggcgaat agataattcg tcgttgtata caaacgtata
      421 aaaaccttgt catatcaacg gttttggcat ttttaaacct ctgtgttttc cacgcatgtt
      481 tgcccttatt taaataattt gccctttttt cgccccgaaa aaaacacaaa aaaataacca
      541 cactcctaaa ttaataggtg gtgtggtttg atcatttata atctatctaa tatcttattt
      601 tcttttcgct tcatgtattg ctccataaat tcatagtcgg gctgattgtt tttagtgggg
      661 agtaatatgc gttctctttt aatacgaaaa tcattaattt cacgattaaa actatacttt
      721 ccttctagtc tacgtagcat attagctata aataaataag taaacttatt taaattatta
      781 ttttttaagt gagtaacgtg atcactagca atgaatctat acgtatgata aaaagtactt
      841 cctacacttc cgctgtttgc tattgttaaa caattatcaa aaactctaat tttttcgcta
      901 tttgaaataa agttatctaa tccgttatta ataccactag aggatatgta aggtgttaat
      961 ccttttattt gattagattt agttaacctt tttcctcgtt gaatagtatt aaaaacttct
     1021 gaagttgaga agggtagcca ctcaacctca tctaactctc gccaatcaga aattttgtgt
     1081 ttttgtttct cttttatatg tgattttaga ttaaaatatt tttctttgat gtatcgttcc
     1141 atgaattcgt aatcaggact attattttta gaaggaagca atattttacg tctttttact
     1201 cttaattggt ttagaccata gccatagtta tatataaatt tctgtttatt gtacatagtt
     1261 gcaataaaca agcctatata tttatttaag cttacgttat agcctatgta aacatttttg
     1321 tgcggtataa aatcttctgc tttataaaga gttagtccaa cagaaccttc tccagtttta
     1381 ataaaaacta aagcgtttcc ttctactttc agttcttcat tattagcgaa acctaccaca
     1441 ccattatttt tatattttgc ccctatacac ggtaactttg aatcgttttt taaattcaat
     1501 tttgaaaagt cagacgtagt cccagattta atactgaata actcatcagt tgtaaacgcc
     1561 ttccattctc tatcactcag tttcattatc gaataaatcc cccctgccgt gcagtttcat
     1621 atcaaattca aatgataagt aatcagctat tgtcttttcg aaatcttcat ctgtaggaat
     1681 ttcttcattg tagtaaaaga agctatgcaa ccattcatcc ttccaagtaa taggggattt
     1741 aactaaaaag ttagtatccg catcttcata atcatttaac acgtttaaaa gatattcttt
     1801 ttttgatttc tcggtaccat ctccaactaa tcccacatgt ttacgcacta tataaccatc
     1861 atcagaaaag tttacaaagt taacacgttt tttgttgtct tgtggaacac ctgcagtgaa
     1921 aattgctata cacggattta ctcccacgcc ataaaaggta tctttattaa gcgtaattac
     1981 tgtttctaac gaatgctttt ctagtatttc gcgcttgtaa ttcttatcat tattagtttt
     2041 accaatcatt gtactctgtg gaacgatagc ggctaactta gcgtcctttt tcattagtga
     2101 caacgtttca ttgataaaac taatttctga taagtgactt aagtttttcg ttttagcttg
     2161 tgaatatggg gggttaatta atgccttagt aatttgattt ttatatagac ctgtttcaac
     2221 atggaatatg tcatcacgtt ttagattact tttaccatca ccacgtaaaa tcatatttgt
     2281 agttgctata gtgaataact tttgttgtaa ttcaattcca tgtaattgat tttgttttat
     2341 ttcagctttt ttgatttcat catttgtttt tcctaacatt ctattcatgg cagctattaa
     2401 aaacccacct gaaccacagc atggatctaa tacataatca ttttcatcaa tatcaattaa
     2461 ttcgcacatc aggtttgtaa tatgtctagg tgttaagact atgcctaatg aattaccatc
     2521 atttcctcca tattttacaa actcaccata aaaattacct agtatatcca tatcagaatg
     2581 tttgatattt t
//
LOCUS       DAMZPP010000213         2554 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMZPP010000213 DAMZPP010000000
VERSION     DAMZPP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.215"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..759
                     /locus_tag="POH73_002706"
     CDS             <1..759
                     /locus_tag="POH73_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDI6888603.1"
                     /translation="HNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQA
                     ANTLKELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNSQEHHLVNKL
                     AAGGFRDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLS
                     FFAEAKSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILE
                     VREDIYGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            complement(895..2157)
                     /locus_tag="POH73_002707"
     CDS             complement(895..2157)
                     /locus_tag="POH73_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDI6888604.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVVLAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHNQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYS
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            2305..2490
                     /locus_tag="POH73_002708"
     CDS             2305..2490
                     /locus_tag="POH73_002708"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDI6888605.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
BASE COUNT          846 a          459 c          378 g          871 t
ORIGIN      
        1 cataatattc atttagtcag tggtcatcca atggctggta gtcataaatc cggtgtacta
       61 aatgctaaaa agcacttatt tgaaaacgct tattatattt tagtctacaa tgagccaaga
      121 aatgagcaag cagcaaacac gttaaaagaa ctgttatcac ctactcttgc taaatttatt
      181 gtaactactg ctgaagaaca cgactacgta acaagcgtcg taagtcattt acctcatatc
      241 gttgcatcta gtttagttca tgttagtcaa aagaacagtc aagaacatca tttagtcaat
      301 aaacttgcag ctggtggttt tcgtgatatc actcgtatag ctagtagtaa tgcacaaatg
      361 tggaaagata tcaccttgag taataaaacg tatattttag aaatgattcg acagctaaaa
      421 agtcagtttc aagatttaga aagactaatt gaaagcaatg attctgaaaa attgttatca
      481 ttttttgccg aagctaaatc gtatcgtgac gcactgcccg ctaaacaact aggtggacta
      541 aatactgcgt atgatctata tgtagatatt ccggatgaat caggtatgat aagcaaagtg
      601 acttatatcc taagtttaca taatatatct ataagcaact taagaatctt agaagtacgc
      661 gaagatatat acggtgcttt aaaaattagt ttcaaaaatc ctaccgatcg agaacgcggt
      721 atgcaagcat tgagtgattt tgattgttat atccaataat tattgagtct cttttgataa
      781 attccatgta ttcttagata ttattgtgaa atctattgtg atatatgaaa tatttatctt
      841 tagagactta ttttatacga atcacagtta tatattcctt aaaccgtata gtcattacat
      901 cttatgtcca gccattaaac ctaatctacc atgttttgta cctgcttctg taaacgatac
      961 tgctttggac acaatacctt tgctgtattt caaatgtaat tggtctatcg ttttagctag
     1021 acattcgtca cgtttgcgtt ggtattcatc ttcaaacaga cttaactgtc gctcatcctc
     1081 attaataaat tgactcaaag atatacttag cgtacgatat agtgcttgtt tattacataa
     1141 tttatctgcg aaatgcatta ctactttata aatatctttt tctaggtttg ttggatcttt
     1201 caaagtatat tgcttatgta caccaccttc atcactatag ccaaaggcaa aatgtatcgt
     1261 tcttgccact ttttttcttg ctcgaactct gctagcaaca tcttcaatta actcttgcat
     1321 tactactttt gcttcgtcaa aatgataatc tctcattaat atttgacttt tgcatatcga
     1381 tggattgcta atcttatgct tttcacgtac tttactctga tctatcccat tcgcatgtag
     1441 atgcatatca acacctaaaa taccgaactc tttttttaaa aatttatatg gatattttgc
     1501 taaatctcct atagtaaaaa tccctctttt attcaatttg gcttctgttc gacgattaat
     1561 accccaaaaa tctctcaagg gctgaattgg ccataatttt gttggtacat cttgatatcg
     1621 ccattcagct ataccatttt gattatgctt agcttcaaca tccatagcaa ttttacttaa
     1681 taacatatta gaaccaatgc ccacagtaca ataaatgcct gtttcttcat aaatttcacg
     1741 tttaagtctt tcgcaaaatg catgtactgt agaactaaat ctatgatagc tatcagtaac
     1801 atccataaaa aattcgtcaa tactatattg gtgtaaatct tcaggtggaa tataacgcaa
     1861 tgcaatctta gaaatagcaa ctgaaacatt aagatattta cgcatacttg gattgataat
     1921 gtaaatatca tttctatgtg gtatttcaaa caatcgcgat cctgtcttga tgcctaattc
     1981 ttttaattta ggtgtcgcag ccaatactac agaaccctga cgcttagtat ctgcaacaac
     2041 agctaacttt gtttctaatg gatctagccc cttttcaata caagaaacac tcgcaaaaaa
     2101 acttttttga tcaatacata gaacatccct atcttctaat aaatgataat tatacactgt
     2161 tattcctccc ttacttgaat tccattatat acgaacactt gttctatttc aaccatttta
     2221 gaacaaatgt tctattattt tacaaatgaa tttttaagta ttacatgata tactcaaatt
     2281 aataatgatt aggaggtttg gatgatgcca atcgtcaatg taaaattatt agaaggtcgt
     2341 tcggatgaac aattaaaaaa tttagttagc gaagttactg acgccgtaga aaaaacaacg
     2401 ggagcaaata gacaagcaat tcacgttgtt atagaagaaa tgaaaccaaa ccattatggt
     2461 gtggctggcg taagaaagtc agatcaataa ttcttcataa gatgcatgcc aattaattct
     2521 ttgaaaacga acaagagtct gggacataaa tccc
//
LOCUS       DAMZPP010000214         2485 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMZPP010000214 DAMZPP010000000
VERSION     DAMZPP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.216"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..794)
                     /locus_tag="POH73_002709"
     CDS             complement(<1..794)
                     /locus_tag="POH73_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDI6888606.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNSDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKDLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGG"
     gene            complement(794..1285)
                     /gene="lspA"
                     /locus_tag="POH73_002710"
     CDS             complement(794..1285)
                     /gene="lspA"
                     /locus_tag="POH73_002710"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDI6888607.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRVLTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
     gene            complement(1534..2331)
                     /locus_tag="POH73_002711"
     CDS             complement(1534..2331)
                     /locus_tag="POH73_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6888608.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDVLGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNDLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKDYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
BASE COUNT          846 a          461 c          313 g          865 t
ORIGIN      
        1 tgaccaccaa tatccaatgt ctttttcggt ccatactttg gatcaccaac taatgggaag
       61 ccaatatatt tcatgtgcac acggatttga tgcgtacgtc ctgtttcaag ttgacattca
      121 acaagcgtat aatctttaaa atgttctagt acgttaaaat gtgtcactgc ttccttacca
      181 tcatcaacaa cagccataga ttgacgatca tttttgtttc taccaattgg cgcatcgatt
      241 gttccgtaat catgaggaat attcccgtga actaaagcga tatattttct tttaacagat
      301 ttatccatta attgttctac aagcccacga tgagcaatat catttttagc aaccattaat
      361 aaaccagaag tatccatatc tatacggtga acaatacctg gacgaatttc tccattaata
      421 cccgataaat ctttaatttg atacattaaa ccattaacta atgtattggt ataatgacct
      481 ggtgatggat gaactaccat gcctttcggt ttataaacaa ctgcaacgtc atcatcttca
      541 taataaatat ctaaatttaa attttcaggt agaatgtcag cttcaaccac ttctttttca
      601 gtgacaacta tatgatcatt aagtttcact ttataattag atttaacaac tttatcgttt
      661 gcaacgacta aacctgcttt aatccaatct tgtatctggt tacgagacca atcactattt
      721 aattcaggca gcaacttatc tacacgcata cctgtttgtt ctttatctgt aatgttaaat
      781 tcataagtct ccattactta acctccttct cctttttatt ggaagtatcc tttaataagg
      841 caataataat taatattaca ccaattgtta aacttgaatc tgcgatatta aatattggaa
      901 aatcataacc aaaaatattt gtatcaataa agtcaacaac ttctcctgtt aaaactctat
      961 caataaagtt tccaagtgca cctgcaaaaa gtaaactaat agcaacttgc ataaacaaat
     1021 tatattgagc atctttaata aagaaatata ctaaggctat taatataata atggtaataa
     1081 taaagaaaaa tgtcattttt ccactcaata ttccccatgc agcaccatta tttcgatgtg
     1141 atgttatgtt taaaaagtgc ggtatcactt caaatgaatc tccaattttc attgtagtag
     1201 ctataatata tttagtaact tggtcaaata taacgacaaa tactgctatt aaaatggaag
     1261 tgccaataaa atattttttg tgcattttcg ttcctccaat caatcgtcca taagacaact
     1321 ctttatatta tagcttacac ctgctaataa aaaaagtaag catattacat taaatctaat
     1381 gttactaact caatacttga taaactacta tgttttgaca ttaaaaatta tgaaagcagc
     1441 ctcatgggaa acctcataag actgccttca tagcttgtat aaaaagatca ttaaaaacga
     1501 ttcattatat ggttcgtcca ttcaaagtat ttactaatct tcaacttcga tatgattaaa
     1561 gcgataatag ataccatccg gaccttgtaa atttaaatgt gttgtttcag gataatgaaa
     1621 gtcaacaatt gctaatccat acgtatcaaa attttctaca cgtttcgttg ctgataccca
     1681 atcattcatg gccaaatgtt gataatagcc attcgatgct aaaaatattg aataatcatc
     1741 catatatgcc gattgctcta atccaaaata atcgaggtag tagtctttta cctctgataa
     1801 atgaattgtc ttaatatgca atgcacctat aatagcgtca tctggaatac ctaaccataa
     1861 caattttgtt gcatgactca ctaatctcgg tacatttaat ggtctaatat caatttttac
     1921 taaatcattt tgcctcgtcc attgcgcttc gtctttatca acataaaatt taaaaccgtt
     1981 tccttccggg tcttcaaaaa ataatgataa cgaagcaggc tgtatacctc cgttaactgg
     2041 aatatcatat tcgcttaaat gaattagtaa attagctaaa tcactaattt gaggtagttt
     2101 aattgcgata tgaaacagtc ccgcttctga cattaaaggt tcacgcccat tttgtaattc
     2161 aacaagtgta atgacatgat tattttgacc tacttcatat tggatcgatg ttaatgtctc
     2221 atttataata tttaatccta atacgtcctc ataaaatggc tttaattcat tcttatctct
     2281 cacatttaaa gtgataccat ttacaaagtt tgcgttactg ttatgataca ttcaattaac
     2341 tcctatcaaa ttcaatatct gtgcttactt ttacttaaaa ttatcgcgaa cataattgag
     2401 aatagcaact ataacgcatt tataagcttc agagaatttg taccattcct gaataatttt
     2461 tattttagta aatcacacat ttttt
//
LOCUS       DAMZPP010000215         2479 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMZPP010000215 DAMZPP010000000
VERSION     DAMZPP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.217"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..885
                     /locus_tag="POH73_002712"
     CDS             <1..885
                     /locus_tag="POH73_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDI6888609.1"
                     /translation="FSNIKLYYSSDDESMGIHLQLTGTGCREFEHHLQQLNKTWQDFF
                     DKCLSVNANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRSGSAINGFNLSDGRS
                     KGATFYIGSKQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMRKAYAVNCAKVL
                     SRTDNISEIVKSILHNNLRFISPPKDGNDKNRKRWPLYRPWALFIKDTEKLNLTTRPT
                     LKSIEDNLDWLCKQVATTLDTVLTAESMAQSEGLLTDTDFLDKILAHSQFNDEHTNRI
                     NHYLEALKQKKHLSKDKC"
     gene            946..2001
                     /locus_tag="POH73_002713"
     CDS             946..2001
                     /locus_tag="POH73_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI6888610.1"
                     /translation="MKIISKYILTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFY
                     SLLAILLILLLASFIKATRANNDSKEAVNKTNMIQKKYEDNANTVQYSPKLKLYADKF
                     IDTYMNISKDSKELESREKELLKYFPSDYKKPEEKTSDTERKLNSKEFYNIKRKDKQT
                     IIQYIVNYDVNITEKKEVKVKKKKKNDKDKDEYETKTEEKQRKVNQNILINIPIKSEN
                     NKYVVVEYPYFTPIPDSQLNKAKMVEDNLKDNKREDNPKAKAFIEDFFNKYSSSKSDD
                     MAYLMDNPEGLEGTREVSQIREIRLYPKGDDYVAKVEILMKDKDSPLENLEHYTLDIT
                     KKDGKYYVKNMTNSIGG"
     gene            2006..2266
                     /locus_tag="POH73_002714"
     CDS             2006..2266
                     /locus_tag="POH73_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888611.1"
                     /translation="MSGLFNFLVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKW
                     ASGKYGHMVILFVVGGFLFLVSKGPEQVFNAIAGVWKMIFGG"
     gene            2278..>2479
                     /locus_tag="POH73_002715"
     CDS             2278..>2479
                     /locus_tag="POH73_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI6888612.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWF
                     ILFLLFWYLLQPVIMSIGGLMFI"
BASE COUNT          922 a          250 c          458 g          849 t
ORIGIN      
        1 ttttcaaaca ttaaattgta ttattcaagt gatgatgaat caatgggcat tcacttacag
       61 ttaactggta caggttgtag agagtttgaa catcatttac aacagttgaa taaaacttgg
      121 caagattttt ttgataaatg tttatcagtt aatgcaaatt ttacaagaat tgatattgca
      181 attgatgatt ataaaacata tttaaaagtg cctttattaa ttaaaaaggc tgaaaaagct
      241 gagtgtgttt cacgttttag atctggaagc gcaataaacg gttttaattt gtcagacgga
      301 agaagtaaag gcgctacttt ttatattggt tctaaacaaa gcaatttata ttgtcgtttt
      361 tatgaaaaga attatgaaca agcgtttaaa cgtcattgtg atgtcgaaga cattgggtta
      421 tggaatcgtt atgaaataca aatgcgaaag gcatatgcag ttaattgtgc gaaagttttg
      481 agtagaacag ataatataag cgaaattgta aagtctatat tgcataataa tttgcgtttt
      541 atctcccctc ctaaagatgg aaatgataaa aatcgtaagc ggtggccatt atatagacct
      601 tgggcgttat ttattaaaga tactgaaaaa ttgaatttaa ctactagacc aacattaaag
      661 tccattgagg ataatcttga ttggttatgt aaacaagttg ctactacttt agatacagtg
      721 ttaactgccg aaagtatggc gcaatctgag gggttgctta cagatactga ttttttagat
      781 aaaattttag cgcattcaca atttaatgat gagcatacga atagaattaa tcattatttg
      841 gaggctttaa aacaaaaaaa gcacttatca aaagataagt gttaaaaaaa taactgggat
      901 gtgattttta ttaaaaattt cgtaaaggat tttagaaaat cgtttatgaa aataataagc
      961 aagtacattt taacacgatt ttctaaaaac ggaaagcatt ttgatattcc taaagaacaa
     1021 cgccgagtaa ttccaaaatt taaaaatcgt cgtaagatga ttgtaatttg tttttactca
     1081 ttattagcta ttttgttaat tcttttatta gcttctttta ttaaagctac aagagcaaat
     1141 aatgatagta aagaagcagt taataaaacg aatatgatcc aaaaaaagta tgaagataat
     1201 gcgaatacag ttcagtatag tcctaaatta aagttatatg ctgataaatt tattgatacg
     1261 tatatgaata tttctaaaga ctcaaaagaa ttagaatcta gagaaaaaga gttattgaaa
     1321 tattttccat cagattataa aaagcctgaa gaaaaaacat cagatactga acgtaaatta
     1381 aatagtaagg aattttataa tattaaacgt aaagataaac aaacgattat acagtatatt
     1441 gttaattatg atgtgaatat tactgaaaaa aaagaagtta aagtaaagaa gaaaaagaaa
     1501 aatgataaag ataaagatga gtatgaaaca aaaacagaag aaaagcaacg taaagtaaat
     1561 caaaatattc taattaatat acctattaag agtgagaata ataagtatgt tgttgttgaa
     1621 tatccttatt ttactccaat tcctgatagt caattaaata aagctaagat ggttgaggat
     1681 aacttgaaag ataataaacg tgaagataat ccgaaagcaa aagcatttat tgaagatttc
     1741 tttaataaat attcttctag taagtctgat gatatggctt atttaatgga taatcctgag
     1801 ggattagaag gaactagaga agtttctcaa ataagagaaa ttagattata tccaaaaggc
     1861 gatgattatg ttgctaaagt tgaaattttg atgaaagata aagattctcc tttggagaat
     1921 ttggaacatt acacattaga tataacgaaa aaagatggta aatattacgt taaaaatatg
     1981 actaattcta ttggaggtta atgttatgag tggtttattt aattttttag ttttaggtgc
     2041 tgaccgtcca tcacttggtg gagttggtga ctggataagt aatgaagtgg gtacgggtat
     2101 tggtttagta gtattggttg ttggtattgt aaaatgggct agtggtaaat atgggcatat
     2161 ggttatatta tttgttgttg gtggcttttt attcttagtt tctaaaggtc ctgaacaggt
     2221 atttaatgcg attgcaggtg tttggaaaat gatttttggc ggttgaggtg attccatttg
     2281 gataaaaaag cttataattt aaaacggact tttgaacaac cgatagtgat gtatgaattt
     2341 acagataaat ttcgtattaa taagggtttt cgtttagatt tttgggctac atttttgatt
     2401 gtatggttta tattattttt gttattttgg tatttattac agcctgtcat tatgtcgatt
     2461 ggtggactta tgtttattt
//
LOCUS       DAMZPP010000216         2473 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMZPP010000216 DAMZPP010000000
VERSION     DAMZPP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.218"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..124)
                     /locus_tag="POH73_002716"
     CDS             complement(<1..124)
                     /locus_tag="POH73_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDI6888613.1"
                     /translation="MPIESFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLR"
     gene            complement(129..602)
                     /gene="ytkD"
                     /locus_tag="POH73_002717"
     CDS             complement(129..602)
                     /gene="ytkD"
                     /locus_tag="POH73_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HDI6888614.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAITRELYEETGAKVKNIHYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            668..925
                     /gene="yidD"
                     /locus_tag="POH73_002718"
     CDS             668..925
                     /gene="yidD"
                     /locus_tag="POH73_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDI6888615.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(922..1923)
                     /gene="menC"
                     /locus_tag="POH73_002719"
     CDS             complement(922..1923)
                     /gene="menC"
                     /locus_tag="POH73_002719"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDI6888616.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELNDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLLSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLNRQDFTQLQLLAREQVLYIEEPFTDISMLDDVVDGTIPPIAL
                     DEKATSLSAIINLIKLYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGVKVVVGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(1928..>2473)
                     /locus_tag="POH73_002720"
     CDS             complement(1928..>2473)
                     /locus_tag="POH73_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDI6888617.1"
                     /translation="FLTATPEMLHARPDTVGMPSANVDVKIKNPNKKGHGELMIKGAN
                     VMNGYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIYDRRKDLIISGGENIYPYQIET
                     VAKQFPGIIDAVCVGHPDDTWGQVPKLYFVSESNISKAQLIAYLSQHLAKYKVPKHFE
                     KVDTLPYTSTGKLQRNKLYRG"
BASE COUNT          742 a          509 c          372 g          850 t
ORIGIN      
        1 ctcgcaaata aactacaatg cgatttactt catgatgtgg tgtcatcatt aaagctttta
       61 cttgtaagtc atctgacaaa tatgtaattt cttcgaattg atgcgtaaaa gattcaattg
      121 gcattcgttt acgtttgata aaatccaagt gattgcaccc tctctacgca ttttaaaatg
      181 gtactatctt gcagtaagaa actccgttgt gcgagttcaa tatcattgat acagttaaac
      241 aacactggcc ctgctgtttc taaataatcg ttcttgctta ccaatgattc aacttcgata
      301 aaatatacat cttttacaaa atcagtttga tcatgtgttt caatggtata ttgcgctatg
      361 taatgaatat ttttaacttt ggcgcctgtt tcttcatata attcacgggt aattgcttca
      421 gcactacttt ccccgcgttc ccttttacca ccaggaaatt caataccccg taaattatgt
      481 ttggtaaaaa gcaattgatt tttaaacgtt ggaatagcta gcacatgatt gccatctgct
      541 atctcattat cctttttaaa tgtcaaatta acttgacgat tatctttatc cctaaacttc
      601 acgcgcatca catccctaca ttgtatgtta atataatagt taattactat cgttggaggc
      661 attaattatg aaaaagatat tcttggcgat gattcatttt tatcaacgtt tcatttcgcc
      721 actcactcca ccaacttgtc gtttttatcc aacatgttca gagtacacta gagaagcgat
      781 tcaataccac ggtgctttca aaggccttta tttaggcatc cgtcgtattt taaaatgtca
      841 tccgcttcat aaaggtggct ttgacccagt tccgttaaaa aaagacaagt cagcaagcaa
      901 gcattcacat aaacataacc attaatatgg ttgtaattga gttatatcca ctaaaggggg
      961 gcgaaattcg agtcgcccct cttttaatat gcctgaatgc gccaccacat cttgttcaaa
     1021 ataataacct gctggtgtaa catctcctgg ataatcacct ttacgagcaa gcatcgctgt
     1081 aaaataacgg cttaaaccat attcatacat gccgccaaca accactttta caccatgact
     1141 tttcaaagta tcaattgccg tttgcacttt atcaatgcca cctagacgaa atggttttaa
     1201 tacaacaact ttcacattgt ataattttat caaattaatt atggccgaca gtgacgtcgc
     1261 cttttcatca agagcaattg gaggtattgt tccatccact acgtcatcaa gcattgagat
     1321 atctgtaaat ggctcttcga tataaagaac ctgttcacgc gctaataact gtaactgtgt
     1381 aaaatcttga cgattcaagg attcatttgc atctataacc aattgaaagt ggaagtctaa
     1441 ttcccgtaac actctaattt gatgcatgat ttgaggcgtc cattttaatt taattctggt
     1501 cggctttgtt gctttcaatg actctagttg tttatttgat aagccgctcg ctgtcgctcc
     1561 atatgctact gaaaatgaaa gcagtacatg aaacatttga tacaatgcca tgacaatagt
     1621 tgcccttgca gcaggcgtat tttccaatga atctactagt tttagtgcag cttcatacgt
     1681 ttcaaatgat ttattcctat tatcttcgaa ccattgctca attacatgtt tcactgacgc
     1741 aattgtttca tgatcatacc aatctgtttg aaaagcgtta cattccccga aatatgcatt
     1801 tcctttatca tcattcaatt cgataaacaa acaatcacga tgcgttaaag tgactttcgg
     1861 tgttacaatt tgtgacttaa atggctcact atatttataa aaatgcaaag ctgtcaactt
     1921 catcaaatca tcctctatac aacttatttc tttgtaattt acctgttgat gtataaggta
     1981 aagtatcaac cttttcaaag tgtttcggta ctttatattt cgctaaatgt tgtgataaat
     2041 atgcaatcaa ttgtgccttt gaaatgttac tttcactgac aaaatataat ttaggtactt
     2101 gtccccaagt atcatcagga tgccctacac atactgcgtc aatgatacct ggaaattgct
     2161 tcgctaccgt ttcaatttga tatggataaa tattttcacc gccactaata attaaatctt
     2221 tacgtctgtc ataaatcatg acataacctt catgatctat ttcagcaatg tcacccgtat
     2281 taaaataacc attttcaaac gtacccgtta aatctgttgg atacaaatat ccattcatca
     2341 cattggcgcc tttaatcatt aattctccat gacctttttt attaggattt ttaattttta
     2401 cgtctacatt ggcacttggc attcctacag tgtcaggacg tgcatgcaac atttccggtg
     2461 ttgctgttaa aaa
//
LOCUS       DAMZPP010000217         2463 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMZPP010000217 DAMZPP010000000
VERSION     DAMZPP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.219"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..113
                     /locus_tag="POH73_002721"
     CDS             <1..113
                     /locus_tag="POH73_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDI6888618.1"
                     /translation="QDNKSFSSNNTTTNTSSNNVDILKRISNLEKAVFGK"
     gene            176..555
                     /gene="rnpB"
                     /locus_tag="POH73_002722"
     ncRNA           176..555
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="POH73_002722"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            764..1909
                     /locus_tag="POH73_002723"
     CDS             764..1909
                     /locus_tag="POH73_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDI6888619.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFRVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            1993..2325
                     /locus_tag="POH73_002724"
     CDS             1993..2325
                     /locus_tag="POH73_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDI6888620.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
BASE COUNT          897 a          332 c          488 g          746 t
ORIGIN      
        1 ctcaggacaa taaaagtttt tcttcgaata atacaacaac aaatacatct tcaaataatg
       61 tagatatttt aaaacgtatt tcaaacttag aaaaagctgt atttggtaaa taattactaa
      121 atacaaaagt ttaactgtct tactaataat gactatgtta taattttaaa agtgatattt
      181 tgggtaatcg ctatattata tagaggaaag tccatgctca cacagtctga gatgattgta
      241 gtgttcgtgc ttgatgaaac aataaatcaa ggcattaatt tgacggcaat gaaatatcct
      301 aagtctttcg atatggatag agtaatttga aagtgccaca gtgacgtagc ttttatagaa
      361 atataaaagg tggaacgcgg taaacccctc gagtgagcaa tccaaatttg gtaggagcac
      421 ttgtttaacg gaattcaacg tataaacgag acacacttcg cgaaatgaag tggtgtagac
      481 agatggttat cacctgagta ccagtgtgac tagtgcacgt gatgagtacg atggaacaga
      541 acatggctta tagaaatatc actactagtt tagctctcct agatgatgga gagctttttt
      601 catgaaaaga acacttaaaa ttaacgcctt atcttgatat aatgacactg ctttgtttta
      661 aaatgataag caaatgcgtt aatgtatcag cgattaaatt tgttggaaat gtataaaaaa
      721 cacaagctaa gaataaaata cctgtataaa aggagaatca tatatgtttc aattacttgc
      781 agtttgtccg atgggattag aagctgttgt tgctagggaa attcaagaat taggctatga
      841 aacaaatgtt gaaaatggcc gtatattttt tgaaggagac gcaagtgcaa ttgtaaaggc
      901 aaatttatgg ttgcgcacag cagaccgaat caaaattgtt gttggacgtt ttaacgcaac
      961 aacatttgac gaattattcg aacaaaccaa agcgctccct tgggaatcta ttattgataa
     1021 agaaggtaac ttcccagttc aaggtagaag cgttaaatca acactacata gtgtgccaga
     1081 ttgtcaggcg ataactaaga aagctattgt tgaacgatta cgtcgcgcat ataacgaaaa
     1141 aggttggtta aatgaatcag gtgcgaaata ccccgttgaa gttgccattt taaaagataa
     1201 tgtattattg actatcgaca catcaggttc tggtttgaac agacgtggtt atagattagc
     1261 acaaggtgaa gcaccaatta aagaaacgtt ggcagcaagt ttaatccgtc ttgcaaactg
     1321 gaaaggtgat acacctttaa ttgatccatt ttgcggttcg ggtacaatag ctatagaagc
     1381 ttgtttaatt gctcaaaata tcgcacctgg ttttaatcga gagttcgtat cagagcaatg
     1441 gaacatcatg ccagcaaata tttatgatga ttaccgtgat gaagcggata agatggctga
     1501 ttatgataaa gaaatcgaag tatatgcttc tgatatcgat cctgaaatgg tagagattgc
     1561 taagcgtaac gctgaagaag tggggttgtc tgatattatt aaatttagag taaaagatgt
     1621 caatacatta acaattgata cagaagaacc ggttgcgtta attggaaatc ctccatatgg
     1681 tgaacgtatt ggtgatcgtg aagaagttga agaaatgtac cgttatattg gtaaactaat
     1741 gaaacaacat ccatttttat ctacatacat tttaacaagt aataaagaat ttgaatattt
     1801 agtagatcgt aaagcaacga agcgacgtaa gttatttaat ggatatatag aatgtactta
     1861 ttatcaatac tggggtaaaa agacagaacg taaaactatt gaaaactaaa gtgtttctaa
     1921 tgcgtgacta aaactagtaa taattatgtt ctcatgataa taggtacttt tgaaaaatgg
     1981 aggagtctat aaatgggtaa aaaaatgggt ctaggtttat ctatagcatt ggttgttatt
     2041 ggtattgccg ttgtatgttt aatgattttt tctagtcaaa aaacgactta ttttggttat
     2101 atgaatagta atacaaatgc agaaaaagtt gtcagtgaaa aagatggatt agtcaaacat
     2161 aatatcaaag tagaaccatc aaatgatttt aaaccgaaaa aaggagactt tgtaaaatta
     2221 gtttctaaag atgatgggaa gacattttat aaacaagaga ttgttaaaca tgatgacgtc
     2281 ccacacggtt taatgatgaa aattcacgac atgcatatga attaataaaa agcatctata
     2341 acgtaatttg gaagaagtaa agtaatcttc ttatacgttt tagatgcttt tttatcttca
     2401 aattcaaaaa atcggacaga tgggagtggg acagaaatga tatttttaca aaatttattt
     2461 cgt
//
LOCUS       DAMZPP010000218         2415 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMZPP010000218 DAMZPP010000000
VERSION     DAMZPP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.220"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            181..1092
                     /gene="rapZ"
                     /locus_tag="POH73_002725"
     CDS             181..1092
                     /gene="rapZ"
                     /locus_tag="POH73_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDI6888621.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKKSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1089..2084
                     /locus_tag="POH73_002726"
     CDS             1089..2084
                     /locus_tag="POH73_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDI6888622.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2193..>2415
                     /gene="whiA"
                     /locus_tag="POH73_002727"
     CDS             2193..>2415
                     /gene="whiA"
                     /locus_tag="POH73_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDI6888623.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVR"
BASE COUNT          894 a          299 c          468 g          754 t
ORIGIN      
        1 gcaatataat attatagacc ctaggacatt gatttatgtc ccaatctcct tttaaatgat
       61 gtatattttt agaaatttaa tctagacata gtttaaaata aatataaaac atcgttgctt
      121 aattttgtca tagaacattt aaattaacat catgaaattc gttttggcgg tgaaaaaata
      181 atggataata atgaaaaaga aaaaagtaaa agtgaattat tagttgtcac aggtttatct
      241 ggcgcaggta aatctttggt tattcaatgt ttagaagaca tgggatattt ttgtgtagat
      301 aatctaccac cagtgttatt gcctaaattt gtagagttga tggaacaagg aaatccatcc
      361 ttaagaaaag tggcaattgc aattgattta agaggtaagg aactatttaa ttcattagtt
      421 gcagtagtgg ataaagtcaa aagtgaaagt gacgtcatca ttgatgttat gtttttagaa
      481 gctagtactg aaaaattaat ttcaagatat aaggaaacgc gtcgtgcaca tcctttgatg
      541 gaacaaggta aaaaatcgtt aatcaatgca attaatgatg agcgagagca tttgtctcaa
      601 attagaagta tagctaattt tgttatagat actacaaagt tatcacctaa agaattaaaa
      661 gaacgcattc gtcgatacta tgaagatgaa gagtttgaaa cttttacaat taatgtcaca
      721 agtttcggtt ttaaacatgg gattcagatg gatgcagatt tagtatttga tgtacgattt
      781 ttaccaaatc catattatgt agtagattta agacctttaa caggattaga taaagacgtt
      841 tataattatg ttatgaaatg gaaagagaca gagattttct ttgaaaaatt aactgatttg
      901 ttagatttta tgatacctgg gtataaaaaa gaagggaaat ctcaattagt aattgccatc
      961 ggttgtacgg gtggacaaca tagatctgta gcattagcag aacgactagg taattatcta
     1021 aatgaagtat ttgaatataa tgtttatgtg catcataggg acgcacatat tgaaagtggc
     1081 gagaaaaaat gagacaaata aaagttgtac ttatcggtgg tggcactggc ttatcagtta
     1141 tggctagggg attaagagaa ttcccaattg atattacagc gattgtaaca gttgctgata
     1201 atggtgggag tacagggaaa atcagagatg aaatggatat accagcacca ggagacatca
     1261 gaaatgtgat tgcagcttta agtgattctg agtcagtttt aagccaactt tttcagtatc
     1321 gttttgaaga aaatcaaatt agcggtcact cattaggtaa tttattaatc gcaggtatga
     1381 ctaatattac gaatgatttc ggacatgcca ttaaagcatt aagtaaaatt ttaaatatta
     1441 aaggtagagt cattccatct acaaatacaa gtgtgcaatt aaatgctgtt atggaagatg
     1501 gagaaattgt ttttggagaa acaaatattc ctaaaaaaca taaaaaaatt gatcgtgtgt
     1561 ttttagaacc taacgatgtg caaccaatgg aagaagcaat cgatgcttta agggaagcag
     1621 atttaatcgt tcttggacca gggtcattat atacgagcgt tatttctaac ttatgtgtca
     1681 atggtatttc agatgcatta attcattctg atgcgcctaa gctatatgta tctaatgtga
     1741 tgacgcaacc tggggaaaca gatggttata gcgtgaaaga tcatatcgac gcgattcata
     1801 gacaagccgg acaaccgttt attgattatg tcatttgtag tacacaaact ttcaatgctc
     1861 aagttttgaa aaaatatgaa gaaaaacatt ctaaaccagt tgaagttaat aaggctgaac
     1921 tagaaaaaga aagcataaat gtaaaaacat cttctaattt agttgaaatt tctgaaaatc
     1981 atttagtaag acataatact aaagtgttat cgacaatgat ttatgacata gctttagaat
     2041 taattagtac tattcctttc gtaccaagtg ataaacgtaa ataatataga acgtaatcat
     2101 attatgatat gataatagag ctgtataaaa atgaaaatag acagtggttc taaggtgaat
     2161 catgttttaa ataagaaagg aatgactgta cgatgagctt tgcatcagaa atgaaaaatg
     2221 aattaactag aatagacgtc gatgaaatga atgcaaaagc agagctcagt gcactgattc
     2281 gaatgaatgg tgcacttagt ctttcaaatc aacaatttgt tataaatgtt caaacggaaa
     2341 atgcaacaac ggcaagacgt atttattcgt tgattaaacg tgtctttaat gtggaagttg
     2401 aaatattagt ccgta
//
LOCUS       DAMZPP010000219         2282 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMZPP010000219 DAMZPP010000000
VERSION     DAMZPP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.221"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..260
                     /locus_tag="POH73_002728"
     CDS             <1..260
                     /locus_tag="POH73_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDI6888624.1"
                     /translation="PKFGKQRPITYIADFSLWKEGKLVEVIDVKGKATEVANIKAKIF
                     RYQYRDANLTWICKAPKYTGQEWMVYEDLVKVRRKRKREMK"
     gene            265..450
                     /locus_tag="POH73_002729"
     CDS             265..450
                     /locus_tag="POH73_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HDI6888625.1"
                     /translation="MQQQAYINATIDIRIPTEVEYQHFDDVDDEKDALADYLYNNPDE
                     ILEYDNLKIRNVNVEVE"
     gene            451..822
                     /locus_tag="POH73_002730"
     CDS             451..822
                     /locus_tag="POH73_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001651750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888626.1"
                     /translation="MAKTARIVRIHDKPYRFSKFEMELIESHGITPGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERERKREAELRRKKPHLFNVPQKH
                     SRDPYWFDVTYNQMFKKWSEA"
     gene            822..1079
                     /locus_tag="POH73_002731"
     CDS             822..1079
                     /locus_tag="POH73_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HDI6888627.1"
                     /translation="MSIISNRKVDMNEIQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVQRAFDLWEG"
     gene            1082..1282
                     /locus_tag="POH73_002732"
     CDS             1082..1282
                     /locus_tag="POH73_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888628.1"
                     /translation="MATQKQVEYVMSLQEQLELEDCEKYTDEQIKAMSHKEVSNVIEN
                     YKTSIRNEELYDECMSFGLPNC"
     gene            1291..1539
                     /locus_tag="POH73_002733"
     CDS             1291..1539
                     /locus_tag="POH73_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888629.1"
                     /translation="MTMTDNARKEYLNQFFGSKRYLYQDNEGVAHIHVVNSTYYFHGH
                     IVPGWQGVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            1580..1828
                     /locus_tag="POH73_002734"
     CDS             1580..1828
                     /locus_tag="POH73_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDI6888630.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEEQESD"
     gene            1821..>2282
                     /locus_tag="POH73_002735"
     CDS             1821..>2282
                     /locus_tag="POH73_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HDI6888631.1"
                     /translation="MTNTLQVKLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDAIASNGY
                     ITPGVFDIKGEIDLSDAIRQYGTYQINEGDKLAQLVIVPIWTPELKQVEEFE"
BASE COUNT          889 a          337 c          522 g          534 t
ORIGIN      
        1 aacctaaatt cgggaaacaa agaccgatta cgtatatagc cgatttctct ttgtggaagg
       61 aagggaaact ggttgaagtt atagacgtta aaggtaaggc gactgaagtt gccaacatca
      121 aagcgaagat attcagatat cagtatagag atgcgaattt aacgtggata tgtaaagcgc
      181 ctaaatacac aggtcaagaa tggatggtat atgaggactt agtgaaagtc agacgtaaaa
      241 gaaaaagaga aatgaagtga tttaatgcaa caacaagcat atataaatgc aacgattgat
      301 ataagaatac ctaccgaagt tgaatatcag cattttgatg atgtggatga tgaaaaagat
      361 gcactggcag attacttata taacaatccg gacgaaatac tagagtatga caatttaaaa
      421 attagaaatg taaatgtaga ggtggaataa atggcgaaaa cagcaagaat tgtaaggata
      481 catgataaac cttataggtt cagtaaattt gaaatggaat taatagaaag tcacggtata
      541 acacctggaa tggtttctaa aagagtaaaa gacggttggg aactacatga agcaatggac
      601 gcaccagaag gtacgcgttt aagtgagtac agagaaaaga aaacaataga aagactggaa
      661 caagctagac tcgaacgcaa attggaaaga gagcgaaaga gagaggctga gctaagaaga
      721 aagaagccac atttgtttaa tgtacctcaa aaacattcac gtgatccgta ctggttcgat
      781 gtcacttata accaaatgtt caagaaatgg agtgaagcat aatgagcata atcagtaaca
      841 gaaaagtaga tatgaacgaa atacaagaca atgttaagca accagcgcac tacacatacg
      901 gcgacattga aattatagat tttattgaac aagttacggc acagtaccca ccacaattag
      961 cattcgcaat aggtaatgca atcaaatact tgtcaagagc gccgttaaaa aacggacacg
     1021 aggatttagc aaaggcgaag ttttacgtcc aaagagcttt tgatttgtgg gagggttaac
     1081 tatggcaact caaaaacaag ttgaatatgt gatgtcatta caggaacaac tggaattgga
     1141 agactgcgaa aaatatacag acgaacaaat taaagcaatg agtcataaag aagttagcaa
     1201 tgtgattgaa aactataaga caagcataag gaacgaagag ctatatgatg aatgcatgtc
     1261 gtttggtcta cctaattgtt aaaaggagtg atgactatga cagataacgc acgcaaagaa
     1321 tacctaaatc aattctttgg atctaagaga tatctgtatc aggataacga aggagtggca
     1381 catatccatg tagtaaacag cacttattac tttcacgggc atatcgtacc aggttggcaa
     1441 ggcgttaaaa agacatttga tactgctgaa gagctcgaaa tatatataaa gcaacatggt
     1501 ttggaatacg aggaacagaa gcaactaact ttattttaga ggagatataa acaataaaat
     1561 tttatggagg aagacactaa tgaataaccg cgaacaaatt gaacaatcag tgatcagtgc
     1621 tagtgcgtat aacggcaatg acacagaggg attgctaaaa gagattgagg acgtgtataa
     1681 gaaagcacaa gcgtttgatg aaatacttga gggtttacct aatgctatgc aagatgcact
     1741 caaagaagat attggtcttg atgaagcagt agggattatg acggggcaag tggtctataa
     1801 atatgaggag gagcaggaaa gtgactaaca cattacaagt aaaactatta tcagaaaatg
     1861 ctagaatgcc cgaacgaaat cataagacgg atgcaggtta tgacatattc tcagctgaaa
     1921 ctgtcgtact tgagccacaa gaaaaggcag tgatcaaaac agatgtagct gtaagcatac
     1981 cagagggcta tgtcggacta ttaactagcc gtagtggtgt aagtagtaaa acgcatttag
     2041 tgattgaaac aggcaagata gacgcgggat atcacggcaa tttagggatt aatatcaaga
     2101 atgacgctat tgcatcgaat ggttatatca caccaggtgt ttttgatatt aaaggagaaa
     2161 tcgatttgag tgacgccata agacaatatg gaacttatca aatcaacgaa ggcgacaaac
     2221 tagctcaatt ggttatcgtg cctatatgga cacctgaact aaagcaagtg gaggaattcg
     2281 ag
//
LOCUS       DAMZPP010000220         2258 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMZPP010000220 DAMZPP010000000
VERSION     DAMZPP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.222"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            401..787
                     /locus_tag="POH73_002736"
     CDS             401..787
                     /locus_tag="POH73_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDI6888632.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSNDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPFLPNINNERLVLVNE"
     gene            780..1076
                     /locus_tag="POH73_002737"
     CDS             780..1076
                     /locus_tag="POH73_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI6888633.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIYGYTPTCGTCKVSERMLDITNEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFQSVTDLLENLK"
     regulatory      1134..1237
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1326..>2258
                     /locus_tag="POH73_002738"
     CDS             1326..>2258
                     /locus_tag="POH73_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDI6888634.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIILITHEMHVIRRICDEVAVMESGK
                     VIEHGPVTQVFENPQHAVTKRFVKEDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVL"
BASE COUNT          775 a          311 c          440 g          732 t
ORIGIN      
        1 agaaaaagta tatctttatg tttgggccca ccccaacttg cacattgctt gtaagctgac
       61 ttaccgtcag cttctgtgtt ggggcccacc ccaacttgca ttgcttgtaa gctgactttc
      121 cgtcagcttc tatgttgggg ccccgctata attgaaaaag gcttgttata ggtgtatttt
      181 cttttggtta actattggta atatatcatt gtagatttta ggatgttgat ttatggttta
      241 gcctttgtct gcttatgtta aatgtcccag ctagtcaaac attgattttg taattattat
      301 attaagctag tttaaatttt ttgtgattta tgttttattc ataccaattt tgtgaaatga
      361 tacagtgaca gatgtttatt gattttaaag ggtgatgatg atggctattg taaataaagt
      421 gataattgtt gaagggaaat ctgataaaaa aagggtgcaa caggttattg cagaaccagt
      481 caatattatt tgtactcatg gaacaatgag tatagataag cttgatgata tgatagaatc
      541 actatatgat aaacaagttt ttgtattagc cgattctaat gacgaaggag atcgaattag
      601 aaattggttt aaacgttatt tgagtgaaag tgaacatata tttattgata aaacttactg
      661 tcaagttgcg aattgcccca aacaatattt ggcgcatgta ctttcaaaac atggctttac
      721 ttgtaagaaa gaaacacctt tcttaccgaa tataaataat gaaaggttag ttttagtaaa
      781 tgaataattc attagacatc aaagatgtaa ctacatttta tgaggaagat aaacatttaa
      841 tctatggtta tacaccaact tgtggtacgt gtaaggtttc agaaagaatg ttagacatta
      901 ctaatgaaat attgcagttg ccattattga aaatagattt aaacttttat cctcagtttt
      961 gtaaagatat gcaaatcatg tctacgccga ttttattgtt gatgaataaa gataaagaag
     1021 taaaacgaat ttatgcattt caatcggtga ctgatttgtt agaaaattta aaatagttat
     1081 tgacgaaatt ttactaaggt gttaggatta atacaaatta aatgatgcgt aaactcttat
     1141 cgagagtggt ggagggatgt gccctacgaa gcccggcaac cgtcttatat agaaatggtg
     1201 ccaattcaca taaagtttta acttttgaag atgagagaaa caatactact attgctttct
     1261 caatttttct atcgatattg agaaagcatt ttttatttta ttaagcaaca cagggaggaa
     1321 tcaacgtgat tgaattaaaa gaagttgtta aagaatatcg gactaaaaat aaagaagtcc
     1381 ttgctgtaga tcacgttaat ttatcgattc gagcaggatc gatttatggc gtcattggtt
     1441 tttctggagc aggaaaaagt actttgattc gaatgtttaa tcatttagaa gcgcctacat
     1501 caggtgaagt tattatagat ggagaccata taggccaatt gtccaaaaat ggattaagag
     1561 caaaaagaca aaaagtaagt atgatcttcc aacattttaa tttgttatgg tcaaggactg
     1621 tgttaaaaaa tattatgttt ccgcttgaaa ttgcaggtgt ccctagaagg agagcaaagc
     1681 aaaaagcatt agaacttgtc gaactcgtcg gtttaaaagg tagagaaaag gcttatccat
     1741 cagagttatc aggcggacaa aagcaacgcg ttgggattgc acgagcgtta gctaatgatc
     1801 caacagtctt gctttgtgat gaggcaacaa gtgcacttga tccgcaaaca acagatgaaa
     1861 ttttagatct actactaaaa attagagaac aacaaaattt aacaattata ctaattacgc
     1921 atgaaatgca tgtcattcgt cgtatttgtg atgaagttgc agttatggaa agtggtaaag
     1981 tgatagaaca tggaccggtg acacaggttt ttgaaaatcc gcaacacgct gtgacaaaac
     2041 gatttgtgaa agaagattta aatgatgatt tcgaaacatc tttaacagaa ttagagccat
     2101 tagaaaaaga tgcttatatc gttagattag ttttcgctgg ttcaacaaca accgagccta
     2161 ttgtatcgag tctatcaact gcctatgata ttaaaattaa tattttagaa gcaaatatta
     2221 aaaatacaaa aaatggaaca gtcggctttt tagttctg
//
LOCUS       DAMZPP010000221         2198 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMZPP010000221 DAMZPP010000000
VERSION     DAMZPP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.223"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..85
                     /locus_tag="POH73_002739"
     CDS             <1..85
                     /locus_tag="POH73_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDI6888635.1"
                     /translation="NSEKPEENWLFKLDDVDKEAFLALPEA"
     gene            86..886
                     /locus_tag="POH73_002740"
     CDS             86..886
                     /locus_tag="POH73_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDI6888636.1"
                     /translation="METLKSNKARLEYLINDMRRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LPAKYIIHTVGPQIRRLPVSKLNQDLLAKCYLSCLKLADQQSLNHIAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            876..1820
                     /locus_tag="POH73_002741"
     CDS             876..1820
                     /locus_tag="POH73_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDI6888637.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEDKQYHIITTNADNAFDAAEYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLKRYNA
                     FLEQHKDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITTALRNDSTTQNNNIGETKDVLNRTD"
     gene            1798..>2198
                     /locus_tag="POH73_002742"
     CDS             1798..>2198
                     /locus_tag="POH73_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDI6888638.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQNIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGARDVIQSGRNDLTLNG"
BASE COUNT          741 a          329 c          468 g          660 t
ORIGIN      
        1 aaactctgaa aaaccagaag aaaattggtt gttcaaattg gatgatgtcg ataaagaagc
       61 attcctggca ttaccggagg cttaaatgga aacgttaaaa tcaaataaag cgagacttga
      121 atatttaatc aatgatatgc gtcgagagag aaatgacaat gacgtattgg taatgccatc
      181 ttcatttgaa gatttgtggg aattatatcg aggtttagca aatgtcaggc cggcattacc
      241 tgtaagtgat gaatatttag ctgtacaaga tgctatgtta agtgatttga atcgtcaaca
      301 tgttacggat ttgaaagatt tgaagccaat aaaaggtgac aatatctttg tttggcaagg
      361 tgatatcacg acgttaaaaa tcgatgcaat tgttaatgct gcgaatagtc gctttctagg
      421 atgtatgcaa gctaatcatg actgcattga taatattatt catacaaaag cgggtgttca
      481 agttcgactt gattgtgcag agattattcg acaacaaggg cgcaatgaag gtgtaggtaa
      541 agccaaaata acacgtggat ataatttgcc agcaaagtat ataattcata cggttggtcc
      601 gcaaatacgt cgattgcctg tttcaaagtt gaatcaggac ttgttagcta aatgctattt
      661 gagttgtctt aaattggctg atcaacaaag tttaaatcat atagcatttt gctgtatatc
      721 tacaggtgta tttgcttttc ctcaagatga agcagcagaa attgctgttc gaacagtaga
      781 aagctatctc aaagaaacaa attcaacatt gaaagtcgtg ttcaatgtat ttacagataa
      841 ggatttacaa ctatataagg aggcatttaa ccgtgatgca gagtagtaaa tggaatgcaa
      901 tgtctctttt aatggatgac aagacaaagc aggctgaagt attgcgtact gcgattgatg
      961 aagcagatgc gatagtgatt ggaattggtg caggcatgtc tgcatctgac gggtttacat
     1021 atgtaggaga gcgttttacg gaaaatttcc cagattttat tgaaaaatat cgcttctttg
     1081 atatgttgca agcgagttta catccttatg gcagttggca agagtattgg gcatttgaga
     1141 gtcgttttat tacattaaac tatttagatc aacctgtagg tcagtcttat ctcgcattaa
     1201 aatccttagt ggaagataaa cagtaccata tcataactac gaatgcagac aatgctttcg
     1261 atgctgctga atatgatatg acgcatgtat ttcatataca aggcgagtat atactacaac
     1321 agtgtagtca gcattgtcat gctcaaacgt atcgcaatga tgatttaatt cgtaaaatgg
     1381 ttgttgcgca acaagatatg cttatacctt gggagatgat tccaagatgt cccaaatgtg
     1441 atgccccaat ggaagtgaat aaacgtaaag cggaagttgg catggttgaa gatgctgaat
     1501 ttcatgcgca actaaaacgt tataatgctt ttctagagca acataaagat gataaagtgt
     1561 tgtatttgga aattggaatt ggttatacta cgccacaatt tgtgaaacat ccttttcagc
     1621 gtatgacacg taaaaatgaa aatgcccttt atatgacgat gaataaaaag gcatatcgca
     1681 ttccgaattc aattcaagaa cgtaccatac atttaactga ggatatctca acattgatta
     1741 caacagcact ccggaacgat agcacaacgc aaaataacaa cattggagag acaaaagatg
     1801 tacttaatag aaccgattag gaatggagaa tatattactg atggtgcgat tgcactcgct
     1861 atgcaagttt atgttaacca gaatatcttt ttagatgaag atattttatt cccttattat
     1921 tgtgatccaa aagtggaaat tggacgtttt caaaatactg ctatagaagt gaatcaagat
     1981 tatatagata aacacagtat tcaagtagtt cgccgagata ctggtggtgg cgctgtgtat
     2041 gttgataaag gtgccgttaa tatgtgttgt attttagaac aagacacttc aatttatggt
     2101 gattttcaac gattttatca accagctata aaggcgttgc atacattagg tgcaagagat
     2161 gtgatacaaa gcggtagaaa tgatttaaca ttgaacgg
//
LOCUS       DAMZPP010000222         2123 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMZPP010000222 DAMZPP010000000
VERSION     DAMZPP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.224"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..291
                     /locus_tag="POH73_002743"
     CDS             <1..291
                     /locus_tag="POH73_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012990921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDI6888639.1"
                     /translation="LQTYVESNNSDVFEYLENGFDTDTLEGIQSSNTDVIANIEMLYQ
                     LATGINEPARELVEGLKLVTEFVQDENATQEDYKALERKLNDLKASYYSLSK"
     gene            999..1343
                     /locus_tag="POH73_002744"
     CDS             999..1343
                     /locus_tag="POH73_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDI6888640.1"
                     /translation="MSEFEVKEKTYNLPNEHRQVLNVIRNTSNKYITKTKLLNQLGYE
                     YNSSNERWLRRVINSLVYDYGYPIGCSYKPSERGYYIITTEQEKQQAMRSIKKLADGS
                     MKRYEALKRIEV"
     gene            1374..2027
                     /locus_tag="POH73_002745"
     CDS             1374..2027
                     /locus_tag="POH73_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888641.1"
                     /translation="MTTTTITGDTWDVYFNDRRYRNLLGDFEDLITETKSLIRQGYKT
                     DVIKNKMDNKALSLQSKFKELGQILLDEHEEKIVEIQQKEKESSYENPQVEMLKRQDI
                     EAKVNLIDAEELFNLVYNANPKTTNVYELNIYKKAIESRLTEDENVRLKPYFDVLVEK
                     VIYPYRNNEEYQKLEYNYNVLRQFGLQNNGQPVIKHSDGDIEIINIQSKYNEVFRNA"
BASE COUNT          853 a          238 c          431 g          601 t
ORIGIN      
        1 ctacaaacat atgtagaatc aaataatagt gatgtgtttg agtacctaga aaatggattt
       61 gatacagata cacttgaggg cattcaatca agtaatactg atgtgatagc gaatattgaa
      121 atgttgtatc agttagctac gggaatcaat gaaccagcac gagagttagt tgagggatta
      181 aaattagtaa ctgagtttgt acaagatgag aatgcgacac aagaggatta caaggcgtta
      241 gaacgtaaat tgaatgatct aaaagcgtct tactatagct tgagtaaata atgttatgag
      301 gggtcacatg tagtgtgtgg ctcctaataa aaaaacggca aggtttgtac aaggtataga
      361 agtttaaaat ggtaaggttt tcggaaggtg ttggctttta aaatccaaaa gtttcccaaa
      421 ggtgcgcaga ctctgagaac aatgaaatag gaaggtgtac aaggttaaaa aagcgaggta
      481 cagaacttta aaatagtaag tttgaggtag aaagaaaaca atgttttata ccagggttgc
      541 aaaatggtga gaaaagggat aaatgcgagc aattgaatca agaagaagtg aaaaccccaa
      601 gattttccgt aggttataag aatagtgcga ttttagatag gtacgaagaa ttgcgtcaag
      661 aaggaaacta aaggtaagtg gtttttgata ttgataaaag taataaggtt ttaataaggt
      721 ataaaaaatt taaaaagata atatatgtta atgtttgtta tttttgaaaa gggcaaaagt
      781 tttattgttc gtattttgtt cgtgtaaaaa atgggaactt aagttctata gaaattaata
      841 cgttttacta caaaatttct tttccttatt tttttaagag ggataggggt gatgctaaac
      901 gcctgatata atgcgattta tagcgaacat aagtttgatt tagatgtgta gaattggtat
      961 aattagagta agcaaacaac agaaagcatg gtgagacaat gagtgaattt gaagtaaaag
     1021 aaaagacgta caacttaccg aatgaacacc gccaagtact caatgtgata agaaatacgt
     1081 ctaataaata tattactaaa acaaagctgc ttaatcaatt gggatatgaa tataattcaa
     1141 gcaatgaacg atggttacga agagtaatca attcattagt atatgattat ggttatccta
     1201 tcggatgcag ttataaacct agtgaacgtg gttattacat cattacgaca gaacaagaaa
     1261 agcaacaagc gatgagaagt attaagaaat tagctgatgg cagtatgaaa cgctatgaag
     1321 ctttgaaacg aattgaagtg taaaacaaaa actaaagaaa gaggtactta taaatgacaa
     1381 ctacaacaat cacgggtgat acgtgggatg tatattttaa tgatagacgt tatagaaatt
     1441 tgttaggaga ttttgaagat ctaataacag aaacgaaatc attaattaga caaggctata
     1501 aaacggatgt tattaaaaat aaaatggata ataaggcttt gagcctacaa tctaaattca
     1561 aagaattagg acaaatatta ttagatgaac atgaagaaaa aatagtagaa atccaacaaa
     1621 aagagaaaga atcttcatat gagaatccac aagttgaaat gttgaaacgt caagacatag
     1681 aggcgaaagt aaatttaatt gatgcagaag aactatttaa tcttgtttat aatgccaatc
     1741 ctaaaaccac taatgtatat gaacttaata tctataaaaa agcgatagaa agtcgtctta
     1801 ctgaagatga aaatgtaagg ttaaaacctt actttgatgt attggtagaa aaggtaattt
     1861 atccatatcg aaataatgaa gaatatcaaa aattagagta taactataat gttttaagac
     1921 agtttgggtt acaaaataac gggcaaccag tcatcaaaca tagtgatggc gatatagaaa
     1981 ttattaacat tcaaagtaag tataacgaag tgttccgtaa cgcttaaatc aaaaatagcc
     2041 tatccaattt gggtaggctc tctttatagg agtgaacgta tgaaactgct taaaacgaag
     2101 aattgtttat attatcgtaa tgg
//
LOCUS       DAMZPP010000223         2089 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMZPP010000223 DAMZPP010000000
VERSION     DAMZPP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.225"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..347)
                     /gene="deoC"
                     /locus_tag="POH73_002746"
     CDS             complement(<1..347)
                     /gene="deoC"
                     /locus_tag="POH73_002746"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDI6888642.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKVFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAA"
     gene            complement(428..1780)
                     /gene="tet(38)"
                     /locus_tag="POH73_002747"
     CDS             complement(428..1780)
                     /gene="tet(38)"
                     /locus_tag="POH73_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDI6888643.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
     gene            complement(1787..>2089)
                     /locus_tag="POH73_002748"
     CDS             complement(1787..>2089)
                     /locus_tag="POH73_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDI6888644.1"
                     /translation="LLEKAVETARDKGVRHHVGNVLSSDIFYNADTTASERWMRMGIL
                     GVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPEERERAFTDMIEIALSLV"
BASE COUNT          720 a          393 c          314 g          662 t
ORIGIN      
        1 gcagctttaa ccactgcttc aatgtcttgt tgtacatcat caaaacgccc atcttttaat
       61 gcgccgatgt tgatgaccat gtcaatttca tctgcaccat tttgaattgc atcttctgtt
      121 tcaaatactt tcgttgcagt tgtcgacgca cctaatggga atcctattac cgtacaaact
      181 agcacctctg aatcagctag tcgctctgct gcatatttaa catgcgttgg attcacacat
      241 acagatttaa aattgtatgc tttcgcttca tcgatgattt gatcgatttg cgtacgtgtt
      301 gactcaggct tcaataaagt gtgatctata tatttctcaa atttcatact tactactcct
      361 cgtgttatat aatctcttta tttaatttta ctataaatac gaatatatct cgcgaattta
      421 tatctattta tttttcagat tgtgtccaac gatttaagat gacgaataaa actaatccga
      481 caatactcat taaacctaaa ataattaaaa taataccgaa atgtgaactt aatgcatcat
      541 tgcctgggaa atttaatgct ttaaaatcga ttaaagccgc agcaatcgca atacctacag
      601 ataccgccac attaataatt aaattataaa aaccaatagc cacacctgtc atattaagat
      661 ctattgtttt aatggcttcg ttaagtaaag gtgcatacat taaagcaaag ctacctgcaa
      721 agaatatcat agaaatgacg aagattgaaa tgtgattacc tactgcaaat gcaggtaaaa
      781 tcaagctcag tgcgattaaa ataattgctg tgataatcgc ttgttttgaa ttaagatatt
      841 cgccgatttt accacttagt gcaccaacga tgactgctac aatataaccc ggtactaata
      901 gcagtgatgt tgtgtctagc tgaagatgat aaatttgttc cattatgaat gggaacgtaa
      961 aaatataacc caattggatg gcatacatta caaatactat aaataaaaat gaagcataac
     1021 gtttattttg gaaaaatgat ttgtttacta atggacgttg cgcattttta atatatagcg
     1081 caaaaacgat aatcgcaatt aaggcaccaa tcatatataa ccaattaaag ttcgtaataa
     1141 acagcataac tgttgtagcg atagttgcca ctagaattaa gccaacaaaa tctaaatgag
     1201 ctttatttgt attattttct tttggtaata atttaaatag gaatggcaac gtaaatacta
     1261 cgattaatgc aattaaaaac atatttgtcc agtgtaaata cgtagaaata aatccacctg
     1321 ataatgtacc gataactaat gacaaggaat aactgctcgt acttaagcca aggtaagtct
     1381 tctggtcctc tttagaaaga tactttgcaa catatatcac atataatgtc tctgcagcag
     1441 ctaaacctgc agtttgaata atacgtccaa ctaaaagtaa tgcgaattga tgttggaaaa
     1501 tgtaaccaat aattgatccg ataatgatta atatcacgcc ataaataaat agtgtgcgta
     1561 tactaatggc atcagataat gaagcgtata ctacagcgcc aataccaatt actaaaccgg
     1621 ctaatgtcgc ttgccaactt actgtcgtta ctgaaatgtt taagtcatca gcaatggcta
     1681 cagaaatcaa tttgaatgag ttgtctataa ccaaactgaa tacaaataag aataataaaa
     1741 taggtactgc tttctttatt ttagaatatt caacattcat aatcatctac accaatgaca
     1801 gtgcaatttc aatcatatct gtaaatgcac gttccctttc ctcaggtgtt gttgacgttt
     1861 catgaattaa atgatcgctc actgtgaaca cacctaatgc ttcgacacca gcgtaaattg
     1921 cattcatgta taatgcagct gattccattt ctacacctaa aatacccata cgcatccaac
     1981 gttcactcgc tgttgtatcc gcgttataga aaatatcact tgataacaca ttacctacat
     2041 gatgacgtac acctttgtca cgtgctgttt caacagcttt ttctaataa
//
LOCUS       DAMZPP010000224         2073 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMZPP010000224 DAMZPP010000000
VERSION     DAMZPP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.226"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..339
                     /locus_tag="POH73_002749"
     CDS             <1..339
                     /locus_tag="POH73_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6888645.1"
                     /translation="GYTTHVDNNDMGNLIVTNKYTPEKPNKPIYPEKPKDKTPPTKPD
                     HSNKVKPTPPDKPSKVDKDDQPKDNKTKPENPLKELPKTGMKIITSWITWVFIGILGL
                     YLILRKRFNS"
     gene            complement(500..964)
                     /gene="bstA"
                     /locus_tag="POH73_002750"
     CDS             complement(500..964)
                     /gene="bstA"
                     /locus_tag="POH73_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HDI6888646.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDTWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIETILESLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNFK"
     gene            complement(1067..1705)
                     /gene="pcp"
                     /locus_tag="POH73_002751"
     CDS             complement(1067..1705)
                     /gene="pcp"
                     /locus_tag="POH73_002751"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDI6888647.1"
                     /translation="MHILVTGFAPFDNQDINPSWEAVTQLENIIGTHTIDKLKLPTSF
                     KKVDTIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHVLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVVGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTQ"
     gene            complement(1858..>2073)
                     /locus_tag="POH73_002752"
     CDS             complement(1858..>2073)
                     /locus_tag="POH73_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888648.1"
                     /translation="DGKPCERYINCANPECNKQILVSEENEAKYLGACSYDCAKHERN
                     RYVQANNISGNEWQQRLTNFDDLHQHA"
BASE COUNT          719 a          355 c          321 g          678 t
ORIGIN      
        1 ggctatacaa cgcatgtgga taacaatgat atgggcaact tgattgtgac gaataaatat
       61 acgccagaaa aaccgaataa accaatctat cctgaaaaac caaaagacaa aacaccacca
      121 actaaacctg atcattctaa taaagttaaa ccaactcccc cagataagcc atcaaaagtg
      181 gataaggatg atcaacctaa agataataaa accaaacctg aaaatcctct aaaagaatta
      241 ccaaaaactg gtatgaagat tataacttca tggattacat gggtatttat aggtatattg
      301 ggattgtatt taattttaag aaaaagattt aactcataaa ccattataat tatttttata
      361 gataaggcta ttcttagttc tatgtataat acatgatatt aataggtcac ttttaatctg
      421 tatgtaagca gactaagagt ggccttttaa aaaataaaaa aatccgttaa cgtgttgtta
      481 acggttaata agcacatatt tatttaaaat tttttaatat acgagacatc tcttcaattt
      541 taccagcatg aagtgggatg tgaatggcat ttaatactaa taactcttct aagttattac
      601 aaccgacaat tggttgtttc aattcaattg ctaaatcatc ttcagttaga tttcgtgcac
      661 gttcaggtaa tgtttggaga ctttctaaaa tcgtttcaat actcggtact tcatccttcc
      721 attcatctgg agacgaacca tttccgaata aaggtctata tatatttaaa tcaatattct
      781 ctttaccagc aacagctaag gctgattcaa aaattgttaa tacatgccca aattgccaat
      841 gaatggtatt agaaaaaggc ttatgataat catcaagtac cttttcaact ttccaagtat
      901 catacacatt tactaaatat cctaaaccca tatcaatgac atcaaatact gtttttgtag
      961 tcattattaa ttccgccccc ataaataaaa catacttccg taagtattac cctattaaca
     1021 aatttgttag tcatttaagg aatgtatttt gtttgtgcat ttataactat tgcgttgtgc
     1081 ctagggctag gtgtaaatcc tcatcattag aaatagcttc aatagctgca gtcaaacctg
     1141 caactatctt ttctaatggc atagatggtg tatcgggttt accaacgact tgctctggta
     1201 tatatggcac atgaataaaa ccgaatcgaa ggtgagggta atgcttatct tgtaaataac
     1261 ctaagtgata aagtacgtga ttacatacga acgtacctgc actatttgaa agtgctccag
     1321 gaagtccttg gttaataata ctttgagtca ttgctttaac tggtaaattt gaaaaataag
     1381 ctggcgcacc gtctaaatga atggcttgat caataggttg aaaatcatca ttatctggaa
     1441 tacgtgcatc atcaatatta atggcgacac gttctggggt aatggcattt ctaccaccag
     1501 cttgtcctat agctagtaca acatcataat gattagatgc caacgtttta tttataatag
     1561 tatctacttt cttaaaagag gttggtagtt ttaatttatc gattgtatgt gtgccaataa
     1621 tattttctag ttgagtcaca gcttcccaag aaggattaat atcttgattg tcaaaaggcg
     1681 cgaaccctgt tactaaaatg tgcattttaa gacaccccgc agtattaaaa tttaatttag
     1741 tataacatgc gcataattaa aaacatatca ggttatgcat gcgcataaaa aaacagcctt
     1801 gaaaaacctt tataaatcgg attttcaaag cgtgttttga aaggtattta attaatacta
     1861 agcatgttga tgtaaatcat caaagtttgt taaacgttgt tgccactcat taccactaat
     1921 attatttgct tgaacataac gattacgctc atgtttagca caatcataag agcatgcacc
     1981 taaatattta gcttcgtttt cttcagaaac taatatttgt ttattacatt ctgggttagc
     2041 gcaattaata taacgttcac atggtttgcc atc
//
LOCUS       DAMZPP010000225         2030 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMZPP010000225 DAMZPP010000000
VERSION     DAMZPP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.227"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..716
                     /gene="mnhD1"
                     /locus_tag="POH73_002753"
     CDS             <1..716
                     /gene="mnhD1"
                     /locus_tag="POH73_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HDI6888649.1"
                     /translation="RTLSLFFSDNVSFSHYVILFLALLTIIFGCVGAVAYANIKKIIL
                     YNVMIAVGVILVGVAMMTESGMIGAIYYTLHDMLVKLALFLLIGIMIKITGTADLRQF
                     GGLIKRYPVLGWSFFIAALSLAGIPPLSGFYGKFFIVQSTFERGFYLSGVIVLLSSLV
                     VLYSVIRIFLQGFFGQPKGYDLNNKVDVKYLTTIAIVAVVITVLYGLSADYLYPMVKA
                     GAETFYNPSTYVKAVLGGK"
     gene            718..1197
                     /gene="mnhE1"
                     /locus_tag="POH73_002754"
     CDS             718..1197
                     /gene="mnhE1"
                     /locus_tag="POH73_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HDI6888650.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            1197..1490
                     /gene="mnhF1"
                     /locus_tag="POH73_002755"
     CDS             1197..1490
                     /gene="mnhF1"
                     /locus_tag="POH73_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HDI6888651.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            1468..1824
                     /gene="mnhG1"
                     /locus_tag="POH73_002756"
     CDS             1468..1824
                     /gene="mnhG1"
                     /locus_tag="POH73_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HDI6888652.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
BASE COUNT          620 a          309 c          344 g          757 t
ORIGIN      
        1 ctagaacatt gagcctattc tttagtgata atgtaagctt ttcacattat gtcattttat
       61 tcttagcact attaacgatc atctttggtt gtgtcggtgc tgttgcatat gccaacatta
      121 aaaaaatcat cctatacaat gtcatgattg ctgtaggtgt gatcttagtc ggcgttgcaa
      181 tgatgacaga gtcaggtatg attggtgcta tttactatac attgcacgac atgttagtaa
      241 aacttgcatt attcttgctt atcggaatta tgattaaaat tacaggcact gctgatttaa
      301 gacaatttgg tggcttaatt aaacgatatc ctgtacttgg ttggtcattc tttatcgcag
      361 cattaagttt agctggcatt cccccactca gtggctttta tggaaaattt ttcattgtcc
      421 aatcaacatt tgaaagagga ttttatttaa gtggtgtcat cgtcttatta tcaagtttag
      481 tcgtattata ttctgtcatt cgcatattcc tacaaggctt tttcggtcaa ccaaaaggat
      541 atgatcttaa taacaaagtc gacgtcaaat atttaactac gattgcaatc gttgcagttg
      601 tgataactgt tttatatgga ttgtcagcag attacttata tccaatggta aaagctggtg
      661 cagaaacatt ctacaatcca tcaacctacg ttaaagctgt attaggaggt aaataacatg
      721 gctgtacaat tagttttaaa ctttattatt gcagtgtttt ggttgtttgt gacaaatagt
      781 tacacaacaa ataactttgt gttaggtttc atctttggcc tagttcttgt ttacctctta
      841 cacagagtgt taccaggtag attttatgtc atcacgctat atcgtattat taaattagtt
      901 attattttct taatcgaact gattaaagct aattttgatg ttttgaaaat tattattaaa
      961 ccttcgatta aaaatgaacc aggattcttt gtttatcaca cagatttaaa aaaagattgg
     1021 caaattgtct tgttgtctaa tttaattaca ttaacacctg gtactgtagt gcttggtgta
     1081 agtgatgatc gtacaaaaat ttatattcat gccatagatt tttcaactaa agagcaagaa
     1141 gtagaaagta ttaaaacatc attagagaag attgtaagag aggtgggcga aatataatga
     1201 atcataatgt tattatcgtt attgcattaa tcatagttgt catttctatg ttagctatgc
     1261 tcattcgcgt tgtgctaggc ccatcacttg ccgatcgtgt tgtcgcatta gatgcgattg
     1321 gtcttcaatt aatggcagtt atagcattat tcagtatttt attaaatatt aaatacatga
     1381 ttgtcgttat tatgatgatt ggtatattag cctttttagg tactgcagta ttctctaaat
     1441 ttatggacaa aggtaaggtg attgaacatg atcaaaatca tactgattag tcttgcactt
     1501 atctttgtta tcatcggtgc tttaattagc gccctagcag ccataggatt attgagactt
     1561 gaagatgtat attcacgtgc acatgctgcc ggaaaagcat caacattagg tgcaatgtca
     1621 ttactatttg gtacgttttt atattttatt gctactcaag gttttgtaaa tatgcaatta
     1681 atcgttgcga ttatctttgt attaattaca ggtcctcttt caagtcatat gattatgaaa
     1741 gcagcatata atattaaaac gccttatact aaaaagacta aagtcgatga aatttctgaa
     1801 gacttaaaag acacaaaatt atagattata taacaaagtc atgaatataa atcaacaaca
     1861 aacagcagta agatgattcc aaattaggaa tgattttact gctgttttct tttgacattg
     1921 ttacctcttt ttcaatgatt ttagtttagt acatacttct ttgactacac cttcgcccta
     1981 tctaggtata tctctttaat ttaattgcct ttcatgtcgt tatgtattat
//
LOCUS       DAMZPP010000226         2027 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMZPP010000226 DAMZPP010000000
VERSION     DAMZPP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.228"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..260
                     /locus_tag="POH73_002757"
     CDS             <1..260
                     /locus_tag="POH73_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDI6888653.1"
                     /translation="PKLDKQRKIEYIADFALYLDDKLIEVIDIKGMPTEVAKLKAKMF
                     RHKYRNIKLNWICKAPKYTGKTWITYEELIKARRERKREMK"
     gene            265..450
                     /locus_tag="POH73_002758"
     CDS             265..450
                     /locus_tag="POH73_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HDI6888654.1"
                     /translation="MQQQAYINATIDIRIPTEVEYQHFDDVDKEKETLADYLYNNPDE
                     ILEYDNLKIRNVNVEVE"
     gene            451..810
                     /locus_tag="POH73_002759"
     CDS             451..810
                     /locus_tag="POH73_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888655.1"
                     /translation="MGSVVIINNKPYKFNNFEKELMAKRGINAGIVSKRVRGCWEFSE
                     ALDAPYGMHLKEYREMKQMEKIKQARLERKLERQRKKEAELRKKKPHLFNVPQKHPRG
                     RYACYLMENDIFVKVKK"
     gene            814..1056
                     /locus_tag="POH73_002760"
     CDS             814..1056
                     /locus_tag="POH73_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888656.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNERVAHIHVANGNYYFHGHIV
                     PGWQGVKKTFDTAEELETYIKQQDLEYEEQKQLTLF"
     gene            1071..1313
                     /locus_tag="POH73_002761"
     CDS             1071..1313
                     /locus_tag="POH73_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDI6888657.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GMTNAIQHPVKEGIELDEAIGIMVSQVIYEYEESEE"
     gene            1313..1555
                     /locus_tag="POH73_002762"
     CDS             1313..1555
                     /locus_tag="POH73_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888658.1"
                     /translation="MSNKSNSEIIAGVLEMSKSDIYIWDVLSEFDSEQEEEILDFIDE
                     NIEEFEIFLNTALIHIVERLKLRIAFATAKEDNDNE"
     gene            1548..>2027
                     /locus_tag="POH73_002763"
     CDS             1548..>2027
                     /locus_tag="POH73_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HDI6888659.1"
                     /translation="MNNTLQVKLLSKNARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDAQVYLTT
                     NEQCFDIQGEMENSFVNNAKKKPFTINDYYEIYKGDKLAQLVIVPIWTPELKQVEEFE
                     "
BASE COUNT          817 a          281 c          430 g          499 t
ORIGIN      
        1 taccaaaact agataaacaa cgaaagattg aatatattgc agacttcgcg ttatatctcg
       61 atgacaaact gattgaagtt atcgacatta aaggtatgcc aaccgaagta gcaaaactta
      121 aagctaagat gttcagacac aaatacagaa acataaaact caattggata tgtaaagcgc
      181 ctaagtatac aggtaaaaca tggattacgt acgaggaatt aattaaagca agacgagaac
      241 gcaaaagaga aatgaagtga tctaatgcaa caacaagcat atataaacgc aacgattgat
      301 ataagaatac ctaccgaagt tgaatatcag cattttgatg atgtggataa agaaaaagaa
      361 acgctggcag attacttata taacaatcct gacgaaatac tagagtatga caatttaaaa
      421 attagaaatg taaatgtaga ggtggaataa atgggcagtg ttgtaatcat taataataaa
      481 ccatataaat ttaacaattt tgaaaaagaa ctaatggcaa agcgcgggat aaacgctgga
      541 attgtttcta aacgtgttag aggttgttgg gagttttcag aagctttaga cgcgccttat
      601 ggcatgcacc taaaagaata tagagaaatg aaacaaatgg aaaagattaa acaagctaga
      661 ctcgaacgca aattggaaag acagcgaaag aaagaggctg agctacgtaa gaagaagcca
      721 catttgttta atgtgcctca gaaacatcca agaggacgtt atgcgtgcta cctgatggaa
      781 aacgacatat tcgtgaaagt taagaagtag atcatgacag ataacgcacg caaagaatac
      841 ctaaatcaat tctttggatc taagagatat ctgtatcaag ataacgaacg agtggcacat
      901 atccatgtag caaacggcaa ttattacttt cacgggcata tcgtaccagg ttggcaaggc
      961 gtaaagaaga cgtttgatac agccgaagag cttgaaacat atataaagca acaggatttg
     1021 gaatatgagg aacagaagca actaacttta ttttagagga gatggaaatg atgaataatc
     1081 gcgaacaaat cgaacaatca gttatcagtg ctagtgccta taacggtaat gacacagagg
     1141 gattactaaa agagattgaa gacgtgtata agaaagcgca agcgtttgat gaaatacttg
     1201 agggaatgac aaatgctatt caacatccag ttaaagaagg tattgaactt gatgaagcaa
     1261 tagggattat ggtaagtcaa gttatctatg aatacgagga gagtgaagaa taatgagtaa
     1321 taaaagtaat tcagaaataa tcgcaggagt attagaaatg agtaaaagcg atatatatat
     1381 ttgggatgta ttaagcgagt ttgacagtga acaagaggaa gaaatactcg attttataga
     1441 tgagaacatt gaagaatttg aaatattcct caataccgca ctaattcata ttgttgaaag
     1501 attgaaattg agaatagcgt ttgctacagc taaggaggac aacgataatg aataacacat
     1561 tacaagtaaa actattatca aaaaatgcta gaatgcccga acgaaatcat aaaacagatg
     1621 caggttatga catattctca gctgaaactg tcgtacttga accacaagaa aaggcagtga
     1681 ttaaaacaga tgttgctgtg agtataccag agggctatgt cgggctgtta actagccgta
     1741 gtggtgtaag tagtaaaacg catttagtga ttgaaacagg caagatagat gcgggatatc
     1801 atggcaattt aggaattaat atcaagaatg atgcacaagt atatttaaca actaacgaac
     1861 agtgttttga tatacaagga gaaatggaaa attcttttgt aaataatgct aagaaaaaac
     1921 cttttactat aaatgattat tacgaaatat ataaaggcga caaactagct caattggtta
     1981 ttgtgcctat atggacacct gaactaaagc aagtggagga attcgag
//
LOCUS       DAMZPP010000227         1979 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMZPP010000227 DAMZPP010000000
VERSION     DAMZPP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.229"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            377..1216
                     /locus_tag="POH73_002764"
     CDS             377..1216
                     /locus_tag="POH73_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6888660.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKTVKSPVRKASQAKKVVQQPVQQASKKVVVKQAPKQTVAKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            1390..>1979
                     /locus_tag="POH73_002765"
     CDS             1390..>1979
                     /locus_tag="POH73_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDI6888661.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIP"
BASE COUNT          746 a          282 c          330 g          621 t
ORIGIN      
        1 ctctttggtt tttttaaagg tagctaaata aattgtaaat tagattttgg aatatgattt
       61 gtttatgaat atttaagtac aattcggtgg tttttgacga aatattatcc tttataaaag
      121 tttcgtttgt taaacaaatc tatgtactgt gcattaagta atattacctt caacgaattt
      181 caatgtattg aattaaatgt tgaaaacgtt gtcaatcatt tgcagtgaat acattttaca
      241 ccgagtaggt agtttgcatt cttttttata ttgttaacca tttgattaca tcgttataac
      301 aatagctttt gacaaaatgt attgtgctat agtatttgca tacttaaaat actaacagca
      361 aaggaatgac agcaagatga aaaaatctct tacagtgacg gtttcgtcag tgttagcttt
      421 tttagcttta aataatgcag cacatgcaca acaacatggc acacaagtaa aaacacctgt
      481 tcaacataat tatgtatcaa atgttcaagc acaaacgcaa tcaccgacaa cttatacagt
      541 agttgctggc gattcattat ataagattgc tttagagcat cacttaacgt tgaatcaatt
      601 atattcatac aatcctggtg taacaccttt aatttttcct ggtgacgtga tttcacttgt
      661 gcctcaaaat aaagtgaaac aaactaaaac ggttaaatca ccagtaagaa aagcaagcca
      721 agctaaaaag gtagtacaac aacctgtaca acaagcgtct aaaaaagtag tagttaagca
      781 agcacctaag caaacagtag ctaagacagt taatgtagca tacaaacctg ctcaagtaca
      841 aaaatcagta ccaactgtac ctgttgcaca taactacaat aaatcagttg ctaacagagg
      901 aaacttatat gcttatggaa actgcacata ttatgctttc gatcgtcgtg cacaattagg
      961 tagaagtata ggaagtttat ggggcaatgc aaataactgg aattacgcag caaaagttgc
     1021 aggatttaaa gtagataaaa caccagaagt tggcgctatt ttccaaacag ctgctggacc
     1081 atatggacat gttggtgttg ttgaatctgt aaatcctaat ggaacaatta ctgtttcgga
     1141 aatgaactat gctggattta atgttaaatc ttcaagaaca attttaaatc caggaaaata
     1201 taattacatc cactaagtaa catatcaaga caagactatc ctcttaacct gtttaagtaa
     1261 caggttgaga ggattttttg gtatcatcta atcagactta tataaagaag atatttaaat
     1321 gatatttaat tgaatgatat gtaaaaagaa agtataattt atgattaatt aatggaggag
     1381 gtaattgaaa tgggtgtaca tcaatatttt aaaagattat cagatatgga aagacttata
     1441 agattacctg gaaaatttaa atattttgaa cacaatgttg cagcgcactc ctttaaagta
     1501 actaaaattg ctcaatatct agcaacagtt gaagaatatc atggacgaaa gattaattgg
     1561 aaaagcttat atgaaaaagc attaaatcat gatttcgctg aagtgtttac tggtgatata
     1621 aaaacacctg ttaaatatgc gagtagtgaa ttaaaaaaat tattttcgca agttgaagaa
     1681 gaaatggtag agacctttat tgaagaagaa attccattac aatatagaga tgtttataag
     1741 caacgactgc aagaaggtaa agatgattca ttagaaggcc aaatactttc agttgctgat
     1801 aaaattgatt tgctttatga aacatttgga gaaatacaaa aacgtaatcc cgaagaatta
     1861 tttttcgaaa tttatgaaat gagtctagaa acaattattc aatttgacca tttagcatct
     1921 gtacaagatt ttattaataa tatcattcca gaaatgttga ctgaaaactt tatacctag
//
LOCUS       DAMZPP010000228         1933 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMZPP010000228 DAMZPP010000000
VERSION     DAMZPP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.230"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(9..467)
                     /locus_tag="POH73_002766"
     CDS             complement(9..467)
                     /locus_tag="POH73_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888662.1"
                     /translation="MADRTNKEIKTGRFIATASIAFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            complement(804..>1933)
                     /gene="eno"
                     /locus_tag="POH73_002767"
     CDS             complement(804..>1933)
                     /gene="eno"
                     /locus_tag="POH73_002767"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDI6888663.1"
                     /translation="LGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGTP
                     NKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDAP
                     IAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTED
                     AVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVDY
                     LEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIENG
                     IGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQI
                     KTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
BASE COUNT          620 a          355 c          287 g          671 t
ORIGIN      
        1 tcaattttct agcgattaaa tgtactttta cgtttgatag aaacaacaag catataaatc
       61 gctaatgcaa ccgaacctat aataacaatt aaaattggca atgttaagta cgcaccgtgt
      121 gcagctttta ttgcttgaat tgtgattgat aaattaatca acgtaaacaa actatttgat
      181 acataaactg caaacataaa ccaccacaca taagtatgtc gattccaaaa agtgatgaag
      241 cctgctagat aagccaaaat atacataata ccagtgaatc taaagctgtt taaaatgtaa
      301 tctatcgctt tatctgaagt gttttgattc gttaaattga gtaatgcttt ggcagtcgca
      361 ttatccaacg aaacaaagta atgaataatc aacaatattg agaatgcgat tgatgcagtt
      421 gcaataaagc gtcctgtttt aatttcttta ttcgttctat cagccatttg tttactcctc
      481 atatatttaa atacgttcta taaaaccttt atttaatttt accttttaat aaaagtgaat
      541 accctgtcat acaatattca tttcaacata aatttattat accagtttaa taatacaagt
      601 taaatatgtg ttattagtta cctcatgatt attagtctgt tatgaataat acttcaaaaa
      661 aaaacctgcc acagtctcaa ctctgtggca ggttataaat aaaagcgtga actttttaag
      721 cgtgaactca aaaactttat tcaatttata ccgacataat aatcctcaac taaaattatg
      781 gcagtactta atgtttaaaa tgattattta tctaagttat agaatgattt gataccgtca
      841 tatttagcag tttcaaataa ttcatcttcg atacgtaata attgattgta tttagcaata
      901 cggtcagtac gtgataatga accagtttta atttgaccag cgtttgtagc aacagcgata
      961 tcagcaattg ttgtatcttc tgtttcacct gaacggtgag aaactactgc tgtgtaacca
     1021 gctttttgag ccatttcgat tgcatcaaat gtttcagtta atgtaccgat ttggttaact
     1081 ttaattaaga ttgagttacc aattccgttt tcaatacctt ttgctaaaat ttcagtgttt
     1141 gttacgaata aatcgtcacc tactaattgt acacggtcac cgatacgttc tgtaagttgt
     1201 ttccaaccat cccagtcgtt ttcgtccata ccgtcttcaa ttgtaatgat aggatatttg
     1261 tctactaatt gttctaagta gtcaacttgt tctgcagctg tacgttttgc accgtgttcg
     1321 ccttcgaact tactgtagtc atatacacca ttttcataga attctgatga tgcacagtca
     1381 aatcctaaga atacttcttc acctggtttg taaccagctg cttcgattgc ttggataatt
     1441 gtttcaacag catcttcagt accttcaaat ttaggagcga aaccaccttc gtcacctact
     1501 gcagtttcta aaccacgttt gcttaaaatt gattttaagt tgtggaaaat ttcagtaccc
     1561 caacgtaatg attctttgaa cgttgtagca cctacaggta aaatcatgaa ttcttggaat
     1621 gcaattggag catctgagtg agaaccacca ttaacgatgt tcatcattgg tactggtaac
     1681 tgcttaccat taaatccacc taaatatttg taaagtggtt gacctaataa gtcagctgct
     1741 gcacgtgcta ctgcaataga tacacctaaa atagcatttg cacctaattt acctttgttt
     1801 ggagtaccgt ctaatgcgat catcatttta tcaatagata cttgatctaa tactgaaaat
     1861 tcaccttcaa taatttctgg tgcgatgatt tcattaacgt tttcaactgc tttagtaaca
     1921 cctttaccta aat
//
LOCUS       DAMZPP010000229         1861 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMZPP010000229 DAMZPP010000000
VERSION     DAMZPP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.231"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(516..674)
                     /locus_tag="POH73_002768"
     CDS             complement(516..674)
                     /locus_tag="POH73_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDI6888664.1"
                     /translation="MSKSSDALFNSLTQSFELIEGVYNELVTDNLKIVMYKPRKESTS
                     GRINRRFK"
     gene            1354..1458
                     /locus_tag="POH73_002769"
     CDS             1354..1458
                     /locus_tag="POH73_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888665.1"
                     /translation="MLLLERTSMSDFEMLMIVLTIIGLVLISTQDHKK"
BASE COUNT          615 a          272 c          295 g          679 t
ORIGIN      
        1 tgtctttctt ctccatatat ggaatgaaga aaatcacaaa tagggcacag agtatagcta
       61 caattgaacc gtatgtcata ttagcatcac cttttatatg gtttatgcat ttaagtatac
      121 tataacaact accgttattt taagtaggag tgtatttgcg ttagagcccc taacaactcc
      181 aaaatcgcat ttgtgatttt gggataatca aacatttcat tccataagta agatatgttt
      241 cactttatat gagtttttta cattcacatt ctgtagtggt tgtaagaaaa ttttttcttt
      301 ctcttaggca gtaataggca atttacctgt actagtatga acttagtagt taaatcgttc
      361 attaagatta aaaaaactta ggaatctctt ccaaatgcaa ttcgtcttgg taagcattaa
      421 tttcatctaa tatttttgtt attgattcaa ctttatcttt ttctagggag gccggaaata
      481 caaggtttta cttgaaattt aaaaccatca gcgaattatt taaatctacg atttattcga
      541 ccacttgtac tttcttttct tggcttatac atgactattt tcaagttatc cgttacaagt
      601 tcattatata caccttctat tagctcaaac gattgtgtta atgaattaaa taaggcatca
      661 cttgatttac tcattgtcat acatgattat aaaacgagaa taataaagta gtagcacacc
      721 tatatatagt ggaatccttt gattatcttt cgttttaaag ttaatgcttt ctttccagtc
      781 aaactgtgct tgatgatcag gttttgtctc gaatcttgtt gtatctttag gcgacatcta
      841 ttgataacat ttcataatat aaggagtaaa ttcatcatgt ttaagtatat aagctctaaa
      901 tgtataatat gcataggtta atccatgttt ttccttcgaa tattgccaaa gtatacgctt
      961 atagtaaaat ttttgttcac ttgtgtctga aagtaatttt tcgaggatag gataatactt
     1021 atcaataata gactgacgat ttctctttca aagttttcat ttttcaatgt ctattttgaa
     1081 gggagcatat cagccgaaaa ggttggtgct cttctttatc agagaataaa aaatgagaaa
     1141 ttaaattttt atatgatgaa caaacgataa caggggaaga aatagctagt tagacaaggg
     1201 tatgtcactc aactgtttat cgagttgatt aagaaatgaa aaaacttata ctatgaatta
     1261 ctgtttaaaa gtgtgcatgt tataatattt attgagcaag ttggatagat ggtggctaat
     1321 ctcttaataa aggggtgatg cctatggtta tagttgttac tcctagaaag gactagcatg
     1381 tctgattttg aaatgctgat gattgtatta acaatcattg gtttagtatt gattagtact
     1441 caagaccata aaaaataacc ttctattcgc tttgaccggc attttgaagg ctatttttaa
     1501 ataaaatata aggtcaccgt ctttttaacg ggctcattag ggtaacatgt ttccgagtgt
     1561 tgcccttttt gtgtttcaag agttaaagat tttcatcttt tgcttctact tactacaaat
     1621 atattttaac acatttttct tatgaattgt agttctgaac ataatcagaa ttaataaaac
     1681 caactttcca tacagcagaa aatacaatta aaagtataga atgtaatcac gaattatata
     1741 aaaagtattg gagacctctt catatataga taattcactt agttatttta gaaagaagcc
     1801 cctaacaact aaagttgaaa aatagaggaa cacagttgga tgctgcgcat caactgcata
     1861 a
//
LOCUS       DAMZPP010000230         1812 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMZPP010000230 DAMZPP010000000
VERSION     DAMZPP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.232"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(824..1645)
                     /locus_tag="POH73_002770"
     CDS             complement(824..1645)
                     /locus_tag="POH73_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6888666.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGSKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGNVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            complement(1663..>1812)
                     /locus_tag="POH73_002771"
     CDS             complement(1663..>1812)
                     /locus_tag="POH73_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6888667.1"
                     /translation="AGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQFIGDWLTN
                     KLDKR"
BASE COUNT          531 a          320 c          262 g          699 t
ORIGIN      
        1 gtctgccata ataaatgcct cctcggaata attaaatgtt atatataata cttacccact
       61 gaaaaattat ctaaacattt tacttaaata atttttgata ttgatttgac gtcattttat
      121 aactagcgaa atagattcat cattaacttg agggagcgga actaaaataa taaagaatca
      181 ctaatgattt attatgaaat ggttcttggg agtgggactg aaatgatatt ttcgcaaaat
      241 ttatttcgtc atccaacccc aacttgcatt gtttgtagaa tttctttttg aaattctctg
      301 tgttggggcc ccgccccccc aacttgcaca ttattgtaag ctggcttttc gtcagcttct
      361 gtattgggac caaccccaac tcgcattgtt tgtagaattt cttttcgaaa tcctctgtgt
      421 tggggccccg ccaacttgca cattattgta tgctggcttt ccatcagctt ctatgttggg
      481 cccaacccca actcgcattg cctgtagaat ttcttttcga aattctctat gttggggccc
      541 cgcaattatt gtaaaaaggt tgttacaatc gtattttcat tcaatcaact actgcttata
      601 tatcattgta aagctttgga cattgattga agtcacagaa gcgtataggc ttcacttaat
      661 acataatgac ttgataatca tttatatata gttattcatc tcttatcata ttaaatcttc
      721 tacatttcat attgattcta attaaaaaaa cccccatcaa agtttgcatt ttttcaatgt
      781 ctactttgat gggagcatat cagtcaatga gcttaattat taattattta gcaggaataa
      841 ctgcaccgtt gtatttttca ttaatgaagt cttgaatatc tttagattgt aatacttcaa
      901 ttaatgcttt gattttctta tcatcttgat gtccttcttt aacagcaatt aagtttgcat
      961 aaggattatc tttcgcactt tctacagcaa tagaatcttt tttaggattt agtttttgtt
     1021 cgattgcaaa gtttgaatta atgataacag catccgcgtc ttcattttga taaattttag
     1081 gtaagaattc tgctgattgt ttattattaa acttaatatc ttttttattc tctgtaatat
     1141 cactaaactt agcatcttca atttttacgc cttttttgat cttaattaaa cctgcatcaa
     1201 cgaagaattt taagaagcgt ccttgttcag ctggattatt agacacataa actgttgcac
     1261 cttttggtaa ttcttttaaa cttttatact ttttagagta tacagccata ggttctaagt
     1321 gaacattacc ggcacttacg attttgtaac ctttatcctt tttctctgtg tttaaatatg
     1381 gtgtatgttg gaaatagttt gcgtcaattt cacctttgtc tagtaattta ttaggcgtag
     1441 tgtaatcgtt aattgtttta atatctagtt cataaccttt tttctctaat aatggttttg
     1501 ctttttctaa aatttcagca tgtggtgctg gtgaagcacc tactgttatt ttcttgtcgt
     1561 cactgccact tttgcttcca ttaccgcaag ctgctaatac aactgcaaat gttaatacta
     1621 aaataagacc aaataatttt ttcataaaat gaaaccccca atttatcgtt tatcaagttt
     1681 atttgtaagc caatccccaa tgaattggat tataaataca ataattaaaa taaaaactgt
     1741 tgatactaaa atgacatcat tttgatttcg agtgaaacct gttaagtatg ctaaatttcc
     1801 taaaccaccg gc
//
LOCUS       DAMZPP010000231         1723 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMZPP010000231 DAMZPP010000000
VERSION     DAMZPP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.235"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..272
                     /locus_tag="POH73_002772"
     CDS             <1..272
                     /locus_tag="POH73_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDI6888668.1"
                     /translation="CSIYFSDLGCPYQRGSIENANGLLRQYYPKGTDFANITQKSLDE
                     AVKQINARPRMIFDYKSSEEMLKYHVSTQNCEPILNDCVRHEPVN"
     gene            complement(564..761)
                     /locus_tag="POH73_002773"
     CDS             complement(564..761)
                     /locus_tag="POH73_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888669.1"
                     /translation="MNCYDEIYNTIKKLIENKEITSYQINKDTGISYGNINAMRRGER
                     RIENLSLKNAKILYEYAKKVL"
     gene            complement(1255..>1723)
                     /locus_tag="POH73_002774"
     CDS             complement(1255..>1723)
                     /locus_tag="POH73_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDI6888670.1"
                     /translation="NPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRIE
                     VRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPST
                     LYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
BASE COUNT          559 a          292 c          251 g          621 t
ORIGIN      
        1 attgtagtat ttattttagt gatttgggtt gtccatatca acgtggttcc attgaaaatg
       61 caaatggact tttaagacag tattatccta aaggtacgga ttttgcaaat ataacacaaa
      121 agtcattaga tgaagctgtt aaacaaatta atgcacgacc tcgaatgata tttgactata
      181 aatcgagtga ggaaatgcta aagtaccatg taagcacaca aaattgtgaa cctatattaa
      241 atgactgtgt gaggcacgaa cccgtcaatt aatataggtt catctaagcg aatggtttac
      301 aatgagcgcg gaaggacagg acgaataaat tttgtatata aaatgcataa tctaaaaaga
      361 agcatactat ttaaatctgt ggtttttgac agatattact agtaaaatgc ttctttttct
      421 tcttctgtac tcttttagta atatttatta aaaaaattta agaaattctc atttttctat
      481 tgacacactg ccatactata aaaacaccca ctcagtcact agtttgggca gttattgtat
      541 gcctattgaa ctcaatgctt atattataat acctttttcg catattcata taagatcttt
      601 gcattcttta agcttaaatt ttctattctt ctttctccac ggcgcatagc attaatatta
      661 ccgtaactta tcccagtatc tttattaatt tgataactcg ttatctcttt gttttctatc
      721 aattttttga ttgtattgta tatttcatca tagcaattca taaattagat gaggcaaaaa
      781 tttttaattt tttagaatat caatagtagt ataactaaaa tgaaaatacc gatcgataaa
      841 caaaaagata ttttttgttt tgtttctctt ttcatatagt attaccccct taataatgag
      901 tagtaaggcc cctattttcg gggtcttacc ttagaacgtt ctgcgaatga attcgatgag
      961 aagtaatatg aatgtggcta ttttcaagta atactcaacg ttttcgcgac gttcttttat
     1021 cacctcatct catcacctcc aaatatatta aaattcatgt gaactaaaat ataaaatggt
     1081 cttccccagc tttaaaaaaa taaatacata aaacatttac ttgaccaaaa cttgaccaaa
     1141 tccatttttt tacaaaagtg gtcaagtgaa attccacctt tccaaatcaa aaaagagcta
     1201 aagcaaaacg ctctagccct tgatattact gatttcccat cattgtttag cgtattaata
     1261 ttgcttcatg tactgatctc tttcccattc agatacttga gttctatagt aatcccattc
     1321 aattgatttt gaattaataa attgattata gatatgattt cctaaagctt ttttaataac
     1381 ttcattttca cgcattgctt ttaatgcagt ataaagtgtt gaaggtaagt cttgaatgcc
     1441 tactgcttca cgttcttcac ggttcatttc gtaaatattt tggttaactg gttctggaac
     1501 ttttaattta tttttaatgc cgtctaaacc agcttctaaa attgcagcta acgccatata
     1561 tgggttcgca gctgggtcaa ctgaacgtac ttcaatacga gttgataatc ctcttgaaga
     1621 tggtacacgg attaatggtg aacggttttt accactccat gcaatataac atggcgcttc
     1681 ataaccaggt actaaacgtt tatatgagtt tactaacggg tta
//
LOCUS       DAMZPP010000232         1540 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMZPP010000232 DAMZPP010000000
VERSION     DAMZPP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.236"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..1520)
                     /locus_tag="POH73_002775"
     rRNA            complement(<1..1520)
                     /locus_tag="POH73_002775"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          340 a          444 c          336 g          420 t
ORIGIN      
        1 ggctaccttg ttacgacttc accccaatca tttgtcccac cttcgacggc tagctcctaa
       61 aaggttactc caccggcttc gggtgttaca aactctcgtg gtgtgacggg cggtgtgtac
      121 aagacccggg aacgtattca ccgtagcatg ctgatctacg attactagcg attccagctt
      181 catgtagtcg agttgcagac tacaatccga actgagaaca actttatggg atttgcttga
      241 cctcgcggtt tcgctgccct ttgtattgtc cattgtagca cgtgtgtagc ccaaatcata
      301 aggggcatga tgatttgacg tcatccccac cttcctccgg tttgtcaccg gcagtcaact
      361 tagagtgccc aacttaatga tggcaactaa gcttaagggt tgcgctcgtt gcgggactta
      421 acccaacatc tcacgacacg agctgacgac aaccatgcac cacctgtcac tttgtccccc
      481 gaaggggaag gctctatctc tagagttgtc aaaggatgtc aagatttggt aaggttcttc
      541 gcgttgcttc gaattaaacc acatgctcca ccgcttgtgc gggtccccgt caattccttt
      601 gagtttcaac cttgcggtcg tactccccag gcggagtgct taatgcgtta gctgcagcac
      661 taaggggcgg aaacccccta acacttagca ctcatcgttt acggcgtgga ctaccagggt
      721 atctaatcct gtttgatccc cacgctttcg cacatcagcg tcagttacag accagaaagt
      781 cgccttcgcc actggtgttc ctccatatct ctgcgcattt caccgctaca catggaattc
      841 cactttcctc ttctgcactc aagttttcca gtttccaatg accctccacg gttgagccgt
      901 gggctttcac atcagactta aaaaaccgcc tacgcgcgct ttacgcccaa taattccgga
      961 taacgcttgc cacctacgta ttaccgcggc tgctggcacg tagttagccg tggctttctg
     1021 attaggtacc gtcaagatgt gcacagttac ttacacatat gttcttccct aataacagag
     1081 ttttacgatc cgaagacctt catcactcac gcggcgttgc tccgtcaggc tttcgcccat
     1141 tgcggaagat tccctactgc tgcctcccgt aggagtctgg accgtgtctc agttccagtg
     1201 tggccgatca ccctctcagg tcggctatgc atcgttgcct tggtaagccg ttaccttacc
     1261 aactagctaa tgcagcgcgg atccatctat aagtgacagc aagaccgtct ttcacttttg
     1321 aaccatgcgg ttcaaaatat tatccggtat tagctccggt ttcccgaagt tatcccagtc
     1381 ttataggtag gttatccacg tgttactcac ccgtccgccg ctaacatcag agaagcaagc
     1441 ttctcgtccg ttcgctcgac ttgcatgtat taggcacgcc gccagcgttc atcctgagcc
     1501 aggatcaaac tctccataaa aattatgatg tttgattagc
//
LOCUS       DAMZPP010000233         1521 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMZPP010000233 DAMZPP010000000
VERSION     DAMZPP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.237"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            96..413
                     /locus_tag="POH73_002776"
     CDS             96..413
                     /locus_tag="POH73_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888671.1"
                     /translation="MPGEKATELLFDSKPNSIVMLHNHPGQSGFSLNDLAVFTINNSI
                     KTMTIVTNKGRIKFISKTEHFKEKVMKKMIANLLIEKSLDVISTKDIERLLKELYNND
                     NII"
     gene            611..925
                     /locus_tag="POH73_002777"
                     /pseudo
     CDS             611..925
                     /locus_tag="POH73_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282612.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            1082..1255
                     /locus_tag="POH73_002778"
     CDS             1082..1255
                     /locus_tag="POH73_002778"
                     /inference="COORDINATES: protein motif:HMM:NF026880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888672.1"
                     /translation="MSTGNLILSDERFDLKDIINFNQIIGKIHIGNVYIETIKEKVHY
                     SKTGAHIVPYIDK"
     gene            1266..1460
                     /locus_tag="POH73_002779"
     CDS             1266..1460
                     /locus_tag="POH73_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888673.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
BASE COUNT          641 a          161 c          269 g          450 t
ORIGIN      
        1 atgccaaaga aaacaatgat agcaatgaag tagcttattt attaaaaggt gctaaagtta
       61 caaaagtata tggcgatcaa aatagtgtat cttttgtgcc aggggaaaaa gcaaccgaat
      121 tattatttga cagtaaaccg aattcaattg ttatgttaca taaccatcct ggacagtcag
      181 gattttcatt gaatgattta gcagttttta ctattaataa ttctattaag actatgacaa
      241 ttgtaactaa taaaggacgt attaaattta taagtaagac agaacatttt aaagaaaaag
      301 taatgaaaaa aatgatagca aacttattaa tagaaaaatc tctagatgta attagtacaa
      361 aagatattga aaggttatta aaagagttat acaataacga taatataata taaagaaata
      421 ggtgattaaa aggtgacaat gatgttagac ggtaagttat ctaaaaaaga attgctcagg
      481 ttgttaactg aaaaaaacga tgaaaaaaat aagggaaaag agaaacaatc taaatagcat
      541 cctttctaca caaaaatata gaaagtggtg ctatttttat atgctttttt aactatttac
      601 tgaaatagca gaatcacaaa agatagctta tcttaaagat ttaggcgaag atggcgaata
      661 taaatatgtt gccaaaatag atagtaaaac atctaaatta tgtcattcac ttaacggaaa
      721 aatatttaaa gttaaagata tgataccagg tgtgaatgcg ccacctatgc atccttggtg
      781 tagaagtacc acagtgccac atgtcggcaa ttggcgagac aagttcttta aagagcgtga
      841 aggtaagtat caagtagaag taaaagaagc aaaattacag gaaaaagcta aaaaccagat
      901 gaaagaaatg attgaaagtg ataaaataaa aatataaata aattgtggga aataaaatag
      961 atatatgtta ggtcatcact tatataatga aaataaaaaa aagagccatt ttaaataaaa
     1021 agaaattgcc tagctataca atactttcta tagatctatt gagtgaattg ttaagagaaa
     1081 aatgtcaaca ggcaatctaa tattaagtga tgagcgattt gatttgaaag atattattaa
     1141 ttttaatcaa attattggaa aaatacatat cggaaatgtg tatattgaaa ccataaagga
     1201 aaaagtgcat tattcgaaga caggtgctca tatagtacct tatattgata agtaggtgaa
     1261 aagtattgag aattgaagat gcatatcgta aagatgttat tatcacgctt ttgaataatg
     1321 aagaatacga agggtttgta actgactatg aaaatgaatt tgagagtgaa acaggaaatc
     1381 ttgttgtaga tatacagacc gattttgctg tttattcgtt tgatgaaact gagataaaaa
     1441 gtattagatt attaaaataa tttctaaata gcatcctttc tacacagata aagagaaagc
     1501 ggtgctattt tttgcgcttt t
//
LOCUS       DAMZPP010000234         1476 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMZPP010000234 DAMZPP010000000
VERSION     DAMZPP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.238"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(218..>1476)
                     /locus_tag="POH73_002780"
     CDS             complement(218..>1476)
                     /locus_tag="POH73_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDI6888674.1"
                     /translation="TTEAYTEALRSTFFHWGFHAWAVYGVVALALAYSQFRKGEPGLL
                     SRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQINGGLHYLFNVPNNTF
                     VQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAALIVGPTVLILNMLTS
                     STGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPFVGVFIARVSKGRSI
                     REFISGVLLVPAIVSFVWFSVFGVLGIETGKKYKEIFDMTPETQLFGVFNHVPFGIVL
                     SLIALLLIASFFITSADSATFVLGMQTTFGSLNPSRMVKVVWGISQALIAFVLLLAGG
                     GNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLGLTLTPNKHRLQEYI
                     KSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT          535 a          292 c          213 g          436 t
ORIGIN      
        1 cttcacttta gctttgtcaa acctctttct attaagttgt cagaaataaa cctatcttaa
       61 aatataaaaa aatcgagaat tcgtagttta ataacgaaat tctcgttctt atcattttga
      121 atatactcaa ttttccacaa aaataaacaa gtagtatatc tgttctagct actagaatga
      181 catactactt gttattaaaa tacttgacta aattttatta gttatctttt ttctctatat
      241 ttctacgtga ctgacgcttt tcaagaatgt cagattcata atcttcttgt tgactcttga
      301 tatattcttg taagcggtgt ttattcggag tcaatgttaa acctaggaat ttacgttcct
      361 ggtttgcatc cttgtagaaa cttaccatca tgagtatgac gacaaaggag aatgggaatg
      421 cacttataat tgcagcactt tgaatcgcat ttaaagcttc agcaccgtta ccgccaccag
      481 ctaataaaag tacaaatgct attaaggcct gtgaaattcc ccaaacaact tttaccattc
      541 tagatggatt taatgaacca aatgttgttt gcattcctaa tacaaatgtt gctgagtcag
      601 cagatgtaat aaagaatgat gcaattaata ataatgcaat caacgataaa acaatgccaa
      661 atggcacatg attaaacact ccaaatagct gtgtttcagg agtcatatca aaaatttctt
      721 tgtatttctt acctgtctcg atgcctaata caccaaagac actaaaccat acaaaactaa
      781 caattgctgg aactagcaag acaccagaaa tgaactctct aattgaacgt ccttttgaaa
      841 ctcgtgcaat aaacacacca acgaatgggc tccaacttaa ccaccaaccc cagtaataaa
      901 gtgtccatga agacatccat tcacgttttt gaggatttaa agcagctgta tcaaaactat
      961 taaacaagaa tgtgtttagt aaactacccg tagagctagt taacatattt aaaataagaa
     1021 cagttggtcc aacaattaaa gcagctacca ttaaaatagt acctaaacca atgttcaagt
     1081 tacttaagta ttgaatacct ttacttaatc cagaccatgc acttgctata aataagatag
     1141 taacaacaat gatgataatc gcttgtacaa acgtattgtt tggaacattg aacaagtaat
     1201 gtaaaccacc attaatttgt agagcaccca tacctaacga aacagctacc ccaacgattg
     1261 tcgcaaatac agataaaacg tcaataaaaa tcccaatagg accttctact ttatcaccta
     1321 aaagaggacg taaagttcta gataataaac ctggttcacc tttacggaat tgcgaatatg
     1381 ccaacgctaa cgcaacaaca ccataaacag cccaagcatg gaatccccaa tggaaaaatg
     1441 ttgaacgtag agcttcagta taagcttcag tagttt
//
LOCUS       DAMZPP010000235         1419 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMZPP010000235 DAMZPP010000000
VERSION     DAMZPP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.239"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            48..1367
                     /locus_tag="POH73_002781"
     CDS             48..1367
                     /locus_tag="POH73_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1181 family transposase"
                     /protein_id="HDI6888675.1"
                     /translation="MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPS
                     ACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIV
                     DESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLP
                     EHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVK
                     TVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPNYT
                     IFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRRSYELVQGLR
                     KDLRLCNWPKFINRLNSVSKKSVSKGVWKVVKYYRKHQRMLRNTIYYPAFNNGAIEGI
                     NNKIKLIKRISFGYRNFNNFKARIMMIFSLYKGEKKKTTKPNNGLAA"
BASE COUNT          549 a          203 c          231 g          436 t
ORIGIN      
        1 tataatttaa agtgaccaaa cccaaactaa aggagacaag tgcgcctatg tgtaatgata
       61 ccttagaatt actaagaata aaagatgaaa atataaaata tataaaccaa gaaattgacg
      121 tcattatcaa aggaaaaaaa gcaacagtgg ttaatgctgt actaacgtat aagccttcgg
      181 cctgttattg ttgtggagtt aaaaatgaag gacaaattca taaacatggt aagcgtgttt
      241 ctcgtattac tttacttaaa actcaagggt ataacacata cctcaactta gctaaacaac
      301 gttttaaatg cttagaatgc aatggcactt ttactgctaa aacgtcaatt gttgatgagt
      361 cgtgttttat ctcaagatgt gttactcaaa aagttataga agaagctact aaagttaaaa
      421 cagagattga tactgcagaa gataactgta tctctccatc tactgtaagt cgtattagaa
      481 ctaaagcggc taattcatta cgaattaaac cgtttaattg tttgccagaa cacatcgcta
      541 tggatgaatt taaaagcgtt aaaaatgtaa ctggatcaat gagtttcatt tttatagata
      601 atgatactca tgatgttata gatattttag aaaatagaac tacaagattc ttgcgtgcct
      661 atttcgagcg attcgattta aaaaatcgac aacaagttaa gacggttact attgacatgt
      721 atgaacccta tgtccgatta tttcgcgacc tatttcctaa tgcagctatt atttttgaca
      781 gattccatat cgttcaacat ttaaataggg aacttaataa gtatcgtgta caagttatga
      841 atgaataccg taataaaaaa ggacctaatt atacaatttt taagaataac tggaaagttc
      901 tattgatgga tactagtaaa accatattta gtaaatacag atggaataaa tcttttaagg
      961 cttataaacg ctcatctgac attgtagaat tcatgctttc aaaagacgat atactacgac
     1021 gctcctacga acttgtccaa ggattaagaa aagatctaag gttatgtaat tggcctaaat
     1081 ttattaatcg tttaaattca gttagtaaaa agtctgtgag taaaggtgta tggaaagtgg
     1141 ttaaatatta tagaaaacat caaaggatgt taagaaatac aatttattac ccagcattta
     1201 ataatggtgc tatagaagga attaataata agataaaatt aatcaagcga atttcttttg
     1261 gttacagaaa tttcaacaac tttaaagcac gtataatgat gattttcagc ttatacaaag
     1321 gagaaaaaaa gaagacaacc aagcccaata atggactggc cgcctaataa taaaaactct
     1381 aaaagttgta atttaaaata gttctttaaa ttatatacc
//
LOCUS       DAMZPP010000236         1337 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMZPP010000236 DAMZPP010000000
VERSION     DAMZPP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.240"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..834)
                     /locus_tag="POH73_002782"
     CDS             complement(<1..834)
                     /locus_tag="POH73_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDI6888676.1"
                     /translation="MNEKTGIDQFMRKEIESLGVSYDEQQSSNVEIAEALKTASKSLS
                     GKVGKPEFLFFSDEFLVVVEDKKDIHKHEMKSERGELILDSPEILKDYAVNGAVHYAR
                     HIIKHTNIVEKVFAVGASGDGHSNKISIHYVDSKSYKYISDINNLEDLKEENIEEFYR
                     VSVLGELPKEERELIEVNKIAADLHEDLRNYGSLEGEKKASVVSAILLALENEEVIFN
                     VDKLQGLQGEGVKDGEILFDAIDKYLRNKSLMPHAKIGELKDNFTFIQNDLTLNRSRE
                     DL"
     gene            complement(1197..>1337)
                     /locus_tag="POH73_002783"
     CDS             complement(1197..>1337)
                     /locus_tag="POH73_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6888677.1"
                     /translation="FDVGQAVYAPGTLIYVFEIIDGWCRIYWNNHNEWIWHERLIVKE
                     VF"
BASE COUNT          418 a          277 c          162 g          480 t
ORIGIN      
        1 taagtcttct cttgatctat ttaacgtcaa atcgttctgt ataaaagtga agttatcttt
       61 taattcacca attttagcat gaggcattaa acttttattt cttaaatatt tatcaatagc
      121 atcaaagagg atttcgccat ctttaacacc ctcaccttga agtccttgta gtttatccac
      181 attaaaaatc acttcttcat tttctaaagc tagtaaaatt gcggacacta cagaagcttt
      241 cttttcacct tcaagcgaac catagttcct taagtcctcg tgcaaatcgg cagcaatttt
      301 attaacttct attaactctc tttcttcttt aggtaactct cctaacacag aaacacgata
      361 aaattcttca atgttttctt ctttcaaatc ctctaagttg tttatatctg aaatatattt
      421 atagcttttg ctatctacat aatgtatgct aattttattc gaatgcccat cacctgatgc
      481 gccaaccgca aaaacctttt caactatatt tgtatgttta ataatgtgcc gcgcataatg
      541 cactgcgcca tttacagcat aatctttcaa tatttctggt gaatctagta ttagttcacc
      601 tcgttcagac ttcatttcat gcttatgtat atctttttta tcctcaacaa cgactaaaaa
      661 ttcatcgcta aaaaataaaa attctggttt acctactttt ccgcttaaac ttttacttgc
      721 tgtttttagc gcttccgcta tttcaacatt gctgctttgt tgttcatcgt aacttacgcc
      781 taaactttcg atttcttttc tcataaattg gtctatacca gttttttcgt tcatatatat
      841 tacctcattt tatcacatgt atagcattat tatagcattt ataacggttt ttaaacatat
      901 aaaaaaccac ccagtaacta gtatgagtgg tgtagcgact gtaacaactc tatgttatca
      961 agatatatgt atcgagtgat gacaaggaag atgtctcctg tgagaccaac agtcagatat
     1021 atggactctg ccgggctata tagttcactc ctactatata tgcatgtaat tataacataa
     1081 aaaaaacagg caagtaccga agtacctgcc tgttatctac atttaaatct tgagagaaat
     1141 gttatctaaa gaataatgtt attatatcac aatacaacat ttaagcaaac attatattaa
     1201 aacacttctt tcacaatcaa tctctcatgc catatccact cattatgatt gttccaataa
     1261 atgcgacacc aaccatctat aatttcaaac acatatatca atgttccagg cgcgtataca
     1321 gcctgtccaa catcgaa
//
LOCUS       DAMZPP010000237         1318 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMZPP010000237 DAMZPP010000000
VERSION     DAMZPP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.241"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..1298
                     /locus_tag="POH73_002784"
     CDS             <1..1298
                     /locus_tag="POH73_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDI6888678.1"
                     /translation="SKRQLSKHLSISQTTVEHAYQLLLDEGYIYSRPRSGYFVSEIES
                     LTILNNQPIPSLFDDDSYKPKASDEAYDYAFNLDEIDTKHFPIELFRKYSKDLYDTNH
                     LNQLRRGHFQGELHLRFQLAFYLFTNRGVICDPNQIIIGSSTEQLVNQLVDLLYTSTF
                     IIEKPSYPPIKNILDKKQVEYEQIEVEDNGINVDEVIKSQKNIVYITPSHQFPTGYVM
                     DLKKRTQLIQWAQEKEERFIIEDDYDSEFRYFGKPIPAIQGLYSRGEKVIYISTFSKS
                     IFPSCRVAYMVLPYSIMKKYHSQNHIEGNTVPVHMQNLIATFISSGGFERHLNKMRRI
                     YRRKLTYILKRLKPYKEQLDIQGAETGMHFTITVKNGLTLQECLDRANKVKLKLQVYN
                     FDDDQDYKKTPKFILGFGGIDDDALKPHVDALIKSLIIK"
BASE COUNT          506 a          198 c          189 g          425 t
ORIGIN      
        1 attctaaaag acaattaagc aaacatttat ctatcagcca aacaaccgta gaacatgcat
       61 atcaacttct attagatgaa ggttacattt attcaagacc tcgctctgga tattttgtgt
      121 ccgaaattga atctttaaca attttaaaca atcaacctat cccttctctt tttgatgatg
      181 attcatataa accaaaagcg agtgatgaag cttatgatta tgcttttaat ttggatgaaa
      241 tagatacgaa acatttccct atagaacttt tcagaaagta ttccaaagat ctttatgata
      301 caaatcattt aaatcaatta cgtagaggac actttcaagg agaattacat ttgcggtttc
      361 aactggcatt ttatttattt actaatcgtg gcgtaatttg tgatcctaat caaattataa
      421 ttggttcatc taccgaacaa ttagtaaatc aattagtcga tttactttat acttcaacgt
      481 ttattatcga aaaacctagt tatccaccaa tcaaaaatat tttagataaa aaacaagttg
      541 aatatgaaca aattgaagtt gaagataatg gaataaacgt agatgaagtt attaaatcac
      601 aaaaaaatat agtatatata acgccttccc atcaatttcc aactggatat gtaatggatt
      661 taaaaaaacg aactcaatta atacaatggg ctcaagaaaa agaagaacga tttattatag
      721 aagatgatta tgattccgag tttagatatt tcggtaaacc tattccagca attcaaggtc
      781 tatattcaag aggagaaaaa gtgatttata tcagtacatt ttcaaagtct atttttccta
      841 gctgtcgtgt tgcatatatg gtgttaccct attctataat gaaaaaatat cattctcaaa
      901 atcacattga aggcaacact gtacctgttc atatgcaaaa ccttattgca acgtttatat
      961 cttccggtgg ttttgaaagg catttaaata aaatgagacg aatttatcgt agaaaactaa
     1021 cctatatttt aaaaagatta aaaccataca aagagcaact tgatattcaa ggtgctgaaa
     1081 caggtatgca ttttacaatt acagtaaaaa atggattaac attacaagag tgtcttgata
     1141 gagcaaataa agtaaagtta aaattacaag tttataattt tgatgatgac caagactata
     1201 agaaaacccc taaatttata ttaggctttg gcggtataga cgatgatgct ttaaaaccgc
     1261 atgtagacgc tctaataaaa tcattaatta taaagtaaca cttctttgac aaaagtta
//
LOCUS       DAMZPP010000238         1270 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMZPP010000238 DAMZPP010000000
VERSION     DAMZPP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.242"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..690
                     /gene="whiA"
                     /locus_tag="POH73_002785"
     CDS             <1..690
                     /gene="whiA"
                     /locus_tag="POH73_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDI6888679.1"
                     /translation="ICRTKMKAKEILDELGILKDGIFTHEIDHSMIQDDEMRRSYLRG
                     AFLAGGSVNNPETSSYHLEIFSQNESHAEGLTKLMNSYELNAKHLERKKGSITYLKEA
                     EKISDFLSLIGGYQALLKFEDVRIVRDMRNSVNRLVNCETANLNKTVSAAMKQVESIK
                     LIDKEIGIENLPDRLREIARIRVEHQEISLKELGEMVSTGPISKSGVNHRLRKLNDLA
                     DKIRNGEQIEL"
BASE COUNT          494 a          158 c          240 g          378 t
ORIGIN      
        1 atttgtcgta caaagatgaa agcgaaagaa attcttgatg aattaggaat tttaaaagac
       61 ggcattttta cgcatgaaat tgatcattca atgattcaag atgacgaaat gagacgtagt
      121 tacttgagag gagcttttct ggcaggtggc tcagtgaata accctgaaac atcttcatac
      181 catttggaaa ttttttctca aaatgagagt catgcagaag gcttaacgaa actaatgaat
      241 agttatgagt tgaatgccaa acatttagag cgaaaaaaag gaagtattac gtatttaaaa
      301 gaagcggaaa agatttcgga ttttcttagt ttgataggtg gctatcaagc gttattaaaa
      361 tttgaagacg tacgtattgt aagagatatg cgtaattctg ttaaccgact cgttaattgt
      421 gaaacggcaa atctaaataa aacagttagt gctgcgatga aacaagttga gagcattaaa
      481 ttgattgata aagaaattgg tattgaaaat ttaccagaca ggttgagaga gattgctaga
      541 attcgagtag aacatcaaga aatttcgttg aaagagcttg gagaaatggt atcaactggt
      601 ccaatttcaa aatcaggtgt aaatcatcga ttaagaaaac ttaatgattt agccgataag
      661 attagaaatg gtgaacaaat agaattataa gtaagaaggt gtttttggta ttgaattatc
      721 aaaaatacct tttttatgat taaaaagtgt ttaatgaata atagtcgact aaccattaaa
      781 ttaagctggg aagtttaatg aaggaaattc aataaccaag cgttacctaa ttttctcatt
      841 aagtagcatc atatatatca atttggtcat aagaaaatat gaatgtgata attaattgag
      901 tccctgaaag tccctgatca tagacaaaaa atcaacataa aaagaatccc gacattgcgg
      961 gattctctgt attgaaaagt attgtatttt attaaaatgc ctccttgaag ggaatcgaac
     1021 ccctatctta agaaccggaa tcttacgtgt tatccattac accacaagga gtaaatgtgt
     1081 taatcaaatc actgtactat gagaagtaaa ataagtacaa tctaaaatag atattatcag
     1141 tttacaaagg aaagagacaa gcgtcaaaca atgtaactat ttaaagtcaa agtgtttgac
     1201 caaatttgac ttaatatgta aaataatgag taacagttat tacaaggagg aaatatagat
     1261 gaatttaatt
//
LOCUS       DAMZPP010000239         1207 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMZPP010000239 DAMZPP010000000
VERSION     DAMZPP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.243"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>1207
                     /locus_tag="POH73_002786"
     CDS             <1..>1207
                     /locus_tag="POH73_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI6888680.1"
                     /translation="IIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQ
                     LKDGVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKIVGGLMLSAGLTAFLTG
                     ITEPLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWD
                     RSHALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDA
                     MGGKENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQI
                     KHDMAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGVGQIIPLSEVPDQVF
                     AGKMMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLN
                     GEGFESLINV"
BASE COUNT          380 a          185 c          256 g          386 t
ORIGIN      
        1 gtattattga acgctcatta attccatttg gtttacatca tattttctat tcaccgttct
       61 ggtttgaatt cggaagttat acaaatcacg caggtgaatt agttcgtggt gaccaacgta
      121 tttggatggc acaattgaaa gatggcgtac catttactgc tggtgcattt actactggta
      181 aatatccatt catgatgttt ggtttaccag cggcggcatt tgctatttat aaaaatgcac
      241 gaccagaacg taaaaaaatc gtgggtggtt taatgttatc agcaggatta actgcatttt
      301 taactggtat cactgagcca ttagaatttt cattcttatt tgtagcacca gtactttatg
      361 gaattcacgt attattagct ggtacatcat tcttagtaat gcatttatta ggcgttaaaa
      421 ttggtatgac attctcaggt ggtttcatag attatatttt atatggttta ttaaactggg
      481 atcgttcaca cgcattatta gttattccag tcggtattgt atatgctatc gtgtattact
      541 tcttattcga ctttgcaatt cgtaagttta aattgaaaac accaggtcgt gaagatgaag
      601 aaactgaaat tcgtaactct agtgtcgcaa aattaccatt tgatgtctta gatgcaatgg
      661 gtggaaaaga aaacattaaa catttagatg catgtattac acgtctgcgc gtagaagtgg
      721 ttgataaatc aaaagtagat gtagcaggta ttaaagcttt aggcgcatca ggtgtattag
      781 aagttggaaa caatatgcaa gctatctttg gtccaaaatc agatcaaatt aaacatgata
      841 tggcgaagat tatgagtggt gaaattacaa agccaagtga aacgacagtg actgaagaaa
      901 tgtcagatga accagttcac gtagaagcac ttggaacaac agacatctat gcaccaggtg
      961 tcggtcaaat cattccatta tcagaagtac ctgatcaagt attcgctggt aaaatgatgg
     1021 gtgatggtgt tggctttatc cctgaaaaag gtgaaattgt agcaccgttt gatggtacag
     1081 tgaaaacaat cttccctacg aaacatgcga taggattaga atctgaaagt ggtgtcgaag
     1141 tacttattca tattggtatc gatacagtga aactgaatgg tgaaggattc gaaagtctga
     1201 ttaacgt
//
LOCUS       DAMZPP010000240         1186 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMZPP010000240 DAMZPP010000000
VERSION     DAMZPP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.244"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            29..346
                     /locus_tag="POH73_002787"
     CDS             29..346
                     /locus_tag="POH73_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HDI6888681.1"
                     /translation="MNWEIKDLMCDIEAVKEKINDVAIKHAWFVEDRFVKNELETKRE
                     HINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKFHEIEKASSENFGEVSDDAQKLK
                     ITE"
     gene            410..>1186
                     /locus_tag="POH73_002788"
     CDS             410..>1186
                     /locus_tag="POH73_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001667297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile element-associated protein"
                     /protein_id="HDI6888682.1"
                     /translation="MNEIKLEYDTHVSVVHYESLDSRSFNSFSKINWSKLVNKLSVPI
                     EANYKYARGVAVYGDIKNGANDQGEIIKKHRNDKNVIYRDVIVLDYDEINDLKQLHEA
                     ISSALSNVAWFWHTSYSHRTEQARIRLYIPLNERISADDYRKYTKVLANKIGHKVDEG
                     SYQPSRCFALPVIQKGHIFIKRVNDCPIMNVDMLEQWSKEFEQSNASPNVIGYTRRDS
                     EYWRELCFGTTEGNRNNALASLIGHLLRCHVNDYIVYSFAL"
BASE COUNT          446 a          154 c          230 g          356 t
ORIGIN      
        1 gggaaatatt gaagtggagg aattataaat gaattgggaa attaaagatt taatgtgtga
       61 tattgaagcg gtaaaagaaa aaatcaatga tgtagctatc aaacatgctt ggtttgttga
      121 agatagattt gtaaaaaatg aattagaaac aaaacgggaa catattaatt tttctgctag
      181 ctatttagaa catcgtatac aaaatgaaca tacagttgag ttattacatg tgtacttaaa
      241 agaattcggt gaacttatac aaaaatttca tgaaatagaa aaagcatcat ctgagaactt
      301 tggcgaggta tcagatgacg cacaaaaatt aaaaatcaca gagtaattta gaaattacac
      361 atgtttatta taacattttt tactctgtga atcactagag gtgcaaaaaa tgaatgaaat
      421 taaattagaa tatgacacac atgtttcagt ggtacattat gaaagtttag actcacgttc
      481 atttaatagc ttttcaaaaa ttaattggag taagttggtt aataaactgt ctgtacctat
      541 agaagcaaat tataagtatg cacgtggtgt tgctgtttac ggtgatatta aaaacggtgc
      601 aaatgatcaa ggtgaaatta tcaaaaagca tcgaaacgat aaaaatgtca tatacagaga
      661 tgtgattgta cttgattatg atgaaataaa tgatttaaag caattacatg aagcaatcag
      721 ctcagcttta agcaatgttg catggttttg gcacacaagt tactcgcaca gaactgaaca
      781 agctagaata cgcctgtata tccctctaaa tgagcgaata agtgcagatg attatcgtaa
      841 atatacaaaa gtattagcaa ataaaattgg ccataaagtg gatgaaggtt catatcagcc
      901 aagtagatgt tttgcgttac cagttattca aaaaggacac atatttatta agcgagtgaa
      961 tgactgtcca attatgaatg ttgatatgct cgaacagtgg tcgaaggagt ttgaacaatc
     1021 aaatgctagt cctaatgtca taggatacac tcgacgcgat agtgagtact ggcgcgagct
     1081 atgctttgga acaaccgaag gcaatcgtaa caatgcacta gctagcttaa ttgggcattt
     1141 attaagatgt cacgttaatg attatattgt ttattcattt gcttta
//
LOCUS       DAMZPP010000241         1110 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMZPP010000241 DAMZPP010000000
VERSION     DAMZPP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.245"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..839
                     /locus_tag="POH73_002789"
     CDS             <1..839
                     /locus_tag="POH73_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HDI6888683.1"
                     /translation="LKGILDARKVLKKEKPDLLFSKGGFVSVPVVIAAKSLNIPTIIH
                     ESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADFIGATIREDLKNGNAHNGYQL
                     TGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQVIHLTGKGLKDAQVKKSGYI
                     QYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPMLLVPLGLDQSRGDQIDNANH
                     FADKGYAKTIDEEQLTAQILLQELNKMEQERTRIINNMKSYEQSYTKEALFDKMIKDA
                     LN"
     gene            856..>1110
                     /locus_tag="POH73_002790"
     CDS             856..>1110
                     /locus_tag="POH73_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDI6888684.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFIYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEAL"
BASE COUNT          421 a          146 c          201 g          342 t
ORIGIN      
        1 tattaaaagg tattcttgat gctcgtaaag ttttgaaaaa agaaaaacct gatctattat
       61 tttcaaaagg tggatttgta tctgtgccag ttgttattgc agccaaatca ttaaatatac
      121 caactattat tcatgaatct gacttaacac caggattagc gaataagata gcacttaaat
      181 ttgccaagaa aatatataca acatttgaag aaacgctaaa ctacctacct aaagagaaag
      241 ctgattttat tggagcaaca attcgagaag atttaaaaaa tggtaatgca cataatggtt
      301 atcaattaac aggctttaat gaaaataaaa aagtgttact cgttatgggt ggaagcttag
      361 gaagtaaaaa attaaatagc attattcgcg aaaacttaga tgcattatta caacaatatc
      421 aagtgataca tttaactggt aaaggattaa aagatgctca agttaaaaaa tcaggatata
      481 tacaatatga atttgttaaa gaggatttaa cagatttatt agcaattacg gacacagtaa
      541 taagtagagc agggtcaaat gcgatatatg agttcttaac attacgtata ccaatgttat
      601 tagtaccatt aggtttagat caatcccgag gcgaccaaat tgacaatgca aatcattttg
      661 ctgataaagg ttatgctaaa acgattgatg aagaacaatt gacagcacaa attttattac
      721 aagagctaaa taaaatggaa caggaaagaa ctcgaattat caataatatg aaatcgtatg
      781 aacaaagtta tacgaaagaa gctttatttg ataagatgat taaagacgca ttgaattaat
      841 ggggggtaat gctttatgag tcaatggaaa cgtatctctt tgctcatcgt ttttacattg
      901 gtttttggaa ttatcgcgtt tttccacgaa tcaagactag ggaaatggat tgataatgaa
      961 gtttatgaat ttatatattc atcagagagc tttattacga catctatcat gcttggggct
     1021 actaaagtag gtgaagtctg ggcaatgtta tgtatttcat tacttcttgt ggcatatctc
     1081 atgttaaagc gccacaaaat tgaagcatta
//
LOCUS       DAMZPP010000242         1020 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMZPP010000242 DAMZPP010000000
VERSION     DAMZPP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.246"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(112..951)
                     /gene="noc"
                     /locus_tag="POH73_002791"
     CDS             complement(112..951)
                     /gene="noc"
                     /locus_tag="POH73_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDI6888685.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEENRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFEQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
BASE COUNT          279 a          197 c          147 g          397 t
ORIGIN      
        1 aacatataaa tataaatttt tatgtttcac gtaaaacaaa aaaagacaaa gctgtaatga
       61 tcttagcttt gtcttttatg tgttagtcat catgtatacg acatcctact actaacgttt
      121 atatattcga atttttattt cataataatc ttcatgatct ttgtctttat gctcaacatg
      181 taatcctgtt tgttgaatcg cttggatact tttgcctacc tcatctcgtg cttgcgtgac
      241 atcttgttca aagcgaaggt tttgtgcttt gactttttcg ggcccagttt tttggcgtac
      301 tctatcttca gtctgtttca catttagttt ttgtgcaatg acttgctcaa tcaacgcttc
      361 ttgttcgcta tcagacaatg ataataccgc tctcgcatgg cgttcagtaa ttttaccttc
      421 tcttaagcga agtagtactt tcggcgccaa cttcaataaa cgcaacttat ttgcaataaa
      481 gctttgactt ttacctaaac tttttgccaa ttcactttgc gttgtatcac caatttccaa
      541 taatttctta taggcttccg cttcttcaac aacagacaaa ttttctcttt gaatattctc
      601 aattaatgcc acaacagccg tctcttcatc atccatatca cgaataataa cgtctgcttg
      661 aggtaaattt agtgattgta ttgctctaaa tcggcgctct ccagcaataa tttcaaacat
      721 atcttcttca atcggtctta caacaatagg ctgtaataaa ccatgttcat gtattgattc
      781 agcaagttct ttaattttat ttggttcaaa tacctgtctt ggttgataac ggttgggaac
      841 gatacgttca atttgaatgg attcaacatt actattgcga ttttcttcaa tatgtccaat
      901 gatgtcatct ttgtttttca aaccaaataa ttttgaaaaa ggttttttca ttatccattc
      961 gctcctttta tgttagttgt aaaggattat gcattatttt tcaagtaaag gagtcttatt
//
LOCUS       DAMZPP010000243         1004 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMZPP010000243 DAMZPP010000000
VERSION     DAMZPP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.247"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            1..305
                     /locus_tag="POH73_002792"
                     /pseudo
     CDS             1..305
                     /locus_tag="POH73_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611933.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            617..997
                     /locus_tag="POH73_002793"
     CDS             617..997
                     /locus_tag="POH73_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888686.1"
                     /translation="MDKEQLKKYIYDYVKEYKEIPIYQLEDLFKEMNHDYIGRTSVTH
                     DKDENIVFWSGWNKITMFALIELVKSEQLDLVYRGSFVMRYLLDGRVPNLPLAICYPE
                     DGQQTDVPSWVPMVLRINKEEKIK"
BASE COUNT          388 a          125 c          193 g          298 t
ORIGIN      
        1 aatagaaata atatcgctgg tgatggctat agttcagtca aaaatggtga catcaaagct
       61 atatataaac gtgattattt atgtatatta ggtcaaactg tacacgataa attaggtcat
      121 gaaatgcaga ctataacagg tcaatggggc aaaaaaggat atttaattaa aggtgaaaaa
      181 gatcgcttgc aaaaaaggtg agtcacaaaa acattaagta tagaggattt gctataaaca
      241 aagaaatgct tgaagaatta ggatttgatt tctcgaattc tcataatcct tattcagatt
      301 attaaatagt tcccaaagtt ctcgataagt tcccgcgaaa aacatacaaa cgggaactat
      361 aagactactt taaccacaag caattaaagt taatagttcc cgaagttccc aataaataat
      421 attattattt attatttgaa aacgaacaaa tgttgttagc tttataccat atatgataga
      481 aaatttttaa cgggtacaac gggaactaag tttatttaaa gtttatatat caatggtttg
      541 actagttccc gataagtatt ttaagtcggg aattcaacgg ggactagttc ccatttaaaa
      601 atattggagg taacacatgg ataaagagca acttaaaaag tatatatacg attatgtaaa
      661 agaatataag gagataccga tatatcagtt agaagatttg tttaaagaaa tgaatcacga
      721 ctatataggg agaaccagtg tcacacacga taaggatgag aatattgtgt tttggagtgg
      781 atggaacaaa attacaatgt ttgcgctgat tgaattagtt aaaagtgaac aacttgattt
      841 agtgtataga ggtagttttg taatgcgtta tttgttggat ggtagagttc ctaacttacc
      901 attagcaatt tgttatccag aagatggaca acaaacggac gtgccctcat gggtgcctat
      961 ggtattaaga ataaataaag aggagaaaat caaatgaaca taga
//
LOCUS       DAMZPP010000244          997 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMZPP010000244 DAMZPP010000000
VERSION     DAMZPP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.248"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(51..755)
                     /locus_tag="POH73_002794"
     CDS             complement(51..755)
                     /locus_tag="POH73_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HDI6888687.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTTIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTAIFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
BASE COUNT          372 a          157 c          138 g          330 t
ORIGIN      
        1 attttctcaa atatcagcct taaaacttat tcttttatgt agtaagtgct ttaatagcct
       61 tgatctaatt tatcaatctt acctttacga taaaatgatt tagcaatata tccaaatggt
      121 acattgaaaa ctgttgaagc taattttagt acgtacgttg taataaatat ttcaaataca
      181 actgtaccag gtaaacttcc gataaatgcg atagctacaa atatagctgt atcaattatt
      241 gagcttaaaa atgtactacc gtatgcacgg atgaaaaacg ttttatcaga actaaatact
      301 tttttaatta gtgaaaagat aaatacatca atatgttgac caataatata tgcgacaatt
      361 gagcctaaag ctatacgtgg cacaacatca aagattgcgt gtaatgcttt ttgtgccata
      421 tcttctggtg caggaataaa atgtaatgac aattgcataa caataatcat aattaatgtt
      481 gatgaaaagc ctaaccaaac tgctcttttt gcaactctac gcccataaat atcgtttaat
      541 atatctgttg ctaaataaat agaagcaaac atgacattac ctaaagttgc tgaaatacca
      601 aagatttcta cagttttaat cacttgtatg ttggcaatgg ttgtgccaat tgcaacccat
      661 gcaattaaac cttgtttacc aaaaaagcga tacataagta ccataagcac gaacgttgca
      721 ataaacgtaa ctagtcctaa aatttcatta tacatattaa aatgtcctcc taaattttga
      781 tcatacgggt gtttagaaac cgctcaataa ataacttact cattttactg aaaaatcgtt
      841 tgcgatgcaa tagcatttga atataaatac attttacgta tagaattgta aaaggtttca
      901 ttcaaatctt agggtcaaaa atgttataat atttttatgt caaatttaaa acagtaacac
      961 ttatttacaa ggttgcaata ttttgaagta ataaagg
//
LOCUS       DAMZPP010000245          986 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMZPP010000245 DAMZPP010000000
VERSION     DAMZPP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.249"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..254
                     /locus_tag="POH73_002795"
     CDS             <1..254
                     /locus_tag="POH73_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDI6888688.1"
                     /translation="APMIVRFSLNIGVVVLTTSSLSFLGLGVTPDVAEWGNILRTGSN
                     YLETHSNLAIVPGICIMFVVLAFNFIGDAVRDALDPRIH"
     gene            271..>986
                     /locus_tag="POH73_002796"
     CDS             271..>986
                     /locus_tag="POH73_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6888689.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTR"
BASE COUNT          339 a          143 c          210 g          294 t
ORIGIN      
        1 ttgcgcctat gattgtacgt ttttcattaa atataggtgt ggttgtatta acaacaagta
       61 gtttaagttt cctaggactt ggtgttacac ctgatgtagc tgaatggggc aacattttac
      121 gtaccggtag taactacttg gaaacgcaca gtaatttagc tattgtacct ggtatttgta
      181 ttatgttcgt cgttttagca tttaatttta taggtgatgc agtgcgtgat gcgctagatc
      241 caagaattca ttaaaaaggt agggatagat gtgaagaaaa tcattagtat cgcaattata
      301 gttttagcgt tggtattaag tggttgtggt gtccctacga aatcagaagt ggctcaaaag
      361 tcatcgaaag ttgaagtgaa aggcgagcga ccaacgatac atttcctagg acaagcaagt
      421 tatgaaaatg atatgaatat cgtcaaagat caattggaaa atgcaggatt taacgtgaag
      481 atgaatatcc aaccagatta tggtagctat cgtacacaac gtcaagccgg caattatgat
      541 atccaaatcg atgactggat gacagtattc ggtgacccga actatgctat gacggcatta
      601 tttagttcta caggatcaaa tagtttattg aaagataaac atgtagacca gttgttaaat
      661 aaagcttcta ctcaaaatga agcagatgtt aaacaaacat ataagcaaat tgaagatgaa
      721 gttgtatttg ataaagggta tatggcgcct ttatatggat caaaaaagaa tttagtatat
      781 gacaataaag tgttagataa aaatagtgtt ggattgccaa attcacgtgc attaatatgg
      841 caacaatttg attacaacaa tagtagagaa cgagatacgc ggccacttgt gatgacacaa
      901 caagacggtg aaattcctac attggatcca atacgttcaa ttgcgccgtc agtatattca
      961 attaatatga atatgtacac aaggtt
//
LOCUS       DAMZPP010000246          976 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMZPP010000246 DAMZPP010000000
VERSION     DAMZPP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.250"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>976
                     /locus_tag="POH73_002797"
     CDS             <1..>976
                     /locus_tag="POH73_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISSau3 family transposase"
                     /protein_id="HDI6888690.1"
                     /translation="SEFDEFRHHRGATSYHPKMMLKIILYAYTQSVFSGRRIEKLLHD
                     SIRMMWLAQNQTPSYKTINRFRVNPNTDALIESLFIQFHSQCLKQNLIDDNSIFIDGT
                     KVEANANRYTFVWKKSIQNHESKLNENSKALYRDLVEEKIIPEIKEDGDSDLTIEEID
                     LIGSHLDKEIEDLNHSIQNEDCTQIRKQTRKKRTEIKKFKKKFDDYSERKSKYEEQKS
                     ILKDRNSFSKTDHDATFMRMKEDHMKNGQLKPGYNLQIATNSQFVLSYDLFQNPTDTR
                     TLIPFLTMIQNTFGYLPEYIVADAGYGSEQNYMAIIDDFNKTPLITYGM"
BASE COUNT          390 a          132 c          155 g          299 t
ORIGIN      
        1 agcgaattcg atgaattcag acatcatcgt ggcgcaacat cctatcatcc aaaaatgatg
       61 ttaaaaatta tcttatatgc atacactcaa tctgtatttt ctggtcgtag aatagagaaa
      121 ttacttcatg acagtattcg aatgatgtgg ttagctcaaa atcaaacacc ttcttataaa
      181 actattaatc gttttagagt aaatcctaat actgatgcat taattgaatc tttatttatt
      241 cagtttcata gtcaatgttt aaagcaaaat cttattgatg ataattcaat ttttattgat
      301 ggtacaaaag tagaagctaa tgccaatagg tatacatttg tgtggaagaa aagtattcaa
      361 aatcacgaat cgaaattgaa cgaaaattca aaagcattat atcgtgactt agttgaagaa
      421 aaaataatac cagagataaa agaagatgga gatagcgatt taacaataga agaaatagat
      481 ttaattggta gtcatttaga taaagaaatc gaagatttaa atcattctat tcagaacgaa
      541 gattgtactc aaattagaaa acagacccgt aaaaaaagaa ctgagattaa gaagttcaaa
      601 aagaaatttg atgattattc cgaaagaaaa agtaaatatg aagaacaaaa atcgattctt
      661 aaagatagaa atagcttttc taaaactgat catgatgcaa cttttatgag aatgaaggaa
      721 gaccatatga aaaatggcca acttaagcca ggatacaatt tacaaatagc gacaaattct
      781 caatttgttt tatcctatga cctatttcaa aacccgacag atactagaac tttaattcca
      841 tttttaacaa tgattcaaaa taccttcggt tatttaccgg agtatattgt agctgatgca
      901 ggttatggta gtgagcaaaa ctatatggct attatagatg attttaataa aacgccactt
      961 attacgtatg gtatgt
//
LOCUS       DAMZPP010000247          962 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMZPP010000247 DAMZPP010000000
VERSION     DAMZPP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.251"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>962
                     /locus_tag="POH73_002798"
     CDS             <1..>962
                     /locus_tag="POH73_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6888691.1"
                     /translation="SKKKQKQIAEQISANQESEKIMIRLHDLGFGPKLSMAIYQFYLG
                     DTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAYNDNERLKAALLYTLEEECIKQG
                     HTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQYLNEEKRLIIDNEQVAIPSLYYS
                     EIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDANQVNYAASQKEALQTAINSKVM
                     LLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVNDDYPVVLAAPTGRASKRLQEST
                     GLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDEMSMVDTWL"
BASE COUNT          363 a          135 c          185 g          279 t
ORIGIN      
        1 tctaaaaaga aacagaaaca aattgcagaa caaatttcag caaatcaaga atctgaaaaa
       61 ataatgattc gtttacatga tttagggttt ggtcctaaac tttcaatggc aatttatcag
      121 ttttatctcg gtgatacact gactatttta gatcgaaatc catatcaatt gatttatgat
      181 attaaaggta ttggttttaa taaagcagat caacttgcta gaaatattgg tatcgcttat
      241 aacgataatg aacgattaaa agctgcgttg ctctatacgt tagaagaaga atgtattaaa
      301 cagggacata catatttgcc tatcaatgtt gttatagatt taacggttga tgtcttaaat
      361 tatcaagatg aagaggtcat cgaacctgaa aaattagacg aaatgttgca atacttaaat
      421 gaggaaaaac gactaattat tgacaatgaa caagttgcaa ttccaagttt gtattattct
      481 gaaatcaaaa gtgttcaaaa cttattcaga ataaagacgc atacaaataa acttacggaa
      541 attgaacagt cagatttaca aatgcatatt ggcgaaatag aggatgcgaa tcaagtgaac
      601 tatgcagctt cacaaaaaga agctttgcaa acggctatca attctaaggt gatgctatta
      661 accggtggac cgggaacagg aaagacgaca gtaattaaag gcattgtaga attatatgct
      721 gagattcatg gcttatcatt agattatgat gattatgtca atgatgatta tccagttgta
      781 ttagcagcgc caacaggaag agcgtccaag agactacaag agtcaactgg attagaagct
      841 atgacgattc atcgtttaat aggttggaat caagacacaa agccagagga tatattagaa
      901 aatgaaatta acgcacgact cataatcatt gatgagatgt ctatggtaga tacttggttg
      961 tt
//
LOCUS       DAMZPP010000248          935 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMZPP010000248 DAMZPP010000000
VERSION     DAMZPP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.252"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..306
                     /locus_tag="POH73_002799"
     CDS             <1..306
                     /locus_tag="POH73_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888692.1"
                     /translation="NRNNIVGDGYDSVNYGDVKAVYKHDFLCIKNETVKNKLGHEMQT
                     ITGQWDKKGYLIKDKKRIQKQVKHKSQRHLGYAIKKEIIEELGFDFSISHNPYTESY"
     gene            616..>935
                     /locus_tag="POH73_002800"
     CDS             616..>935
                     /locus_tag="POH73_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888693.1"
                     /translation="MDKEQLKKYIYEYVKEYKEIPIYQLEDLFKEINHDYIGRTSITH
                     DKDENIVFWSGWNKITMFALIELVKSEQLDLVYRGSFIMRYLLDGRVPNLPLAICYPE
                     DGQQT"
BASE COUNT          386 a          105 c          161 g          283 t
ORIGIN      
        1 aatagaaata atattgtagg tgacggttat gattcagtaa attacggaga tgttaaagcg
       61 gtatataaac atgattttct ttgtattaaa aacgaaactg ttaaaaataa attaggacac
      121 gaaatgcaga cgattacagg gcaatgggac aaaaaaggtt atttaataaa agataaaaaa
      181 agaattcaaa aacaagtaaa acataaatct caaaggcatc tgggttatgc gattaaaaaa
      241 gaaataattg aagaactagg atttgatttc tcgatttcgc ataatccata tacagaaagt
      301 tattagtaca cacaagtaca cactcaattt taaaatatgt gtactcgata aattcaataa
      361 tatcaacagt tacatgcaga tagtacacga agtacacaat gtacacatat aaatatatag
      421 tcatagttaa aattaataaa ttaattatcg tagatttcta aataatatac aactatcact
      481 aaaatttctg tgtactttgt gtactaatta tcacgaggct atatatatca atgttttaac
      541 cagtacacga aagtaaaaaa aggtgtgtat ttaactgtgt atggtacaca atcaatataa
      601 atatggaggt tacacatgga taaagaacaa cttaaaaagt atatatacga atatgtgaaa
      661 gaatataagg agataccgat atatcagtta gaagatttgt ttaaagaaat aaatcacgac
      721 tatataggga gaactagtat cacacacgat aaggatgaga atattgtgtt ttggagtggg
      781 tggaacaaaa ttacaatgtt tgcgttgatt gaattagtta aaagtgaaca acttgattta
      841 gtgtatagag gtagttttat aatgcgttat ttgttggatg gtagagttcc taatttacca
      901 ttagctattt gttatccaga agatggacaa caaac
//
LOCUS       DAMZPP010000249          911 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMZPP010000249 DAMZPP010000000
VERSION     DAMZPP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.253"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..>911)
                     /locus_tag="POH73_002801"
     CDS             complement(<1..>911)
                     /locus_tag="POH73_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI6888694.1"
                     /translation="GIVLGFATLFKNPTIMGSLADQHTFWFKFWSVIESGGWVIFTHM
                     EVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINAILTQWPHTFGANLEKGVENVP
                     GLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRLPEMVGVFQGLTFVVTISFFVM
                     LPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYHFLERVLIPTGLHHFIYAPIEV
                     GPVVVNHGLKAEWLQHLNEFAKSSKPLKEQFPYGFMLQGNGKVFGCLGIALAMYATTP
                     KENRKKVAALLIPATLTAVVVGITEPL"
BASE COUNT          309 a          186 c          145 g          271 t
ORIGIN      
        1 tctaatggct cagtaatacc tacaacgact gctgttaatg tggcaggaat aagtagtgcc
       61 gccacctttt tacgattttc tttaggtgtc gtagcataca ttgctaaggc aatacctaaa
      121 catccaaata ctttcccatt tccttgaagc ataaagccat aagggaattg ctcttttaat
      181 ggcttactac tcttagcaaa ctcatttaaa tgttgtaacc attcagcttt caagccgtga
      241 ttgactacta ctggtccaac ttcaattggc gcataaataa agtgatgtaa tcctgttggt
      301 attaatacac gttctaagaa gtgatacaac catacaccga tatagcctga agcaatgata
      361 aagtgttgca ttgagccaat accgtgctga acagttggcc aaataacaca agtaattgct
      421 gctaatggta acatcacaaa aaatgagatt gtaactacaa atgttaaacc ttggaatact
      481 ccaaccattt ctggtaatcg tttactatag tatctattgt gaatccaagt aataatagct
      541 gaaataataa taccacctag aatattcgta tccaatgtgg caatacctgc aattgatttt
      601 aaaccaggta cattttcaac gcctttttct aaattagcgc caaacgtatg tggccattga
      661 gttaaaatgg catttataaa tgtattaaac attaagtaac ccatcaatgc tgcaagtgct
      721 gcatgacctg gtgctttttt agctaaagaa agtggtaaac ctacaacaaa tacaacttcc
      781 atatgcgtaa aaataaccca gccaccagat tctattactg accaaaattt aaaccaaaac
      841 gtatgttgat ctgctaaact ccccatgatt gtaggatttt taaataatgt cgcaaagcca
      901 agcactatcc c
//
LOCUS       DAMZPP010000250          880 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMZPP010000250 DAMZPP010000000
VERSION     DAMZPP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.254"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            91..>880
                     /locus_tag="POH73_002802"
     CDS             91..>880
                     /locus_tag="POH73_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSau1 family transposase"
                     /protein_id="HDI6888695.1"
                     /translation="MSYNHLTLTERARIEVLRQENYSLRSIARKLKRSVSTISREISR
                     NNLNQSYQAETAQKNYETKRKLCGRPTRFTPELGNIIKYYLKCHWSPEQIVGRLLQNQ
                     ICFKTIYRWINSNMINFELISCLRQKGKRQKPKETRGKFNIGRPISQRPKEIKKRNTF
                     GHWEADTIVSSRGKSKGCIATFAERKSRYYYCVLMPDRSSNSMETAINNLIKHLPKGA
                     VKTITVDRGKEFSCYQNIENQFNINVYFADPYSAWQRGTNENTNG"
BASE COUNT          328 a          160 c          137 g          255 t
ORIGIN      
        1 acacctataa aaattcataa aaaatgccca taattggcac tttgttgtaa gatgttaata
       61 accacaaaaa cacatacaag gagtgccaac atgagctata accatcttac actaactgaa
      121 cgcgcacgta tagaggtttt acgtcaagaa aattattctt taagatctat tgcacgtaaa
      181 ttaaaacgat ctgtatctac tatctcacgt gaaattagta ggaataattt aaatcagtca
      241 tatcaagctg aaacagctca aaaaaattat gaaaccaaaa gaaaactttg tggtcgtcct
      301 actcgattta ctccagaact tggtaatatc attaaatatt atttaaaatg ccactggtct
      361 cctgaacaaa tcgtaggtcg tctattacaa aaccaaatct gttttaaaac catctatcga
      421 tggattaact ctaatatgat taatttcgaa cttatttcat gccttcgtca aaaaggaaaa
      481 agacagaaac ctaaagaaac tagaggtaag tttaatattg gccgtcctat ttcacaacgt
      541 cctaaggaaa taaagaaacg taatacattc ggtcattggg aagctgacac aattgtatcc
      601 agcagaggca aaagtaaagg gtgcattgct actttcgcag aaagaaaatc acgttattat
      661 tattgtgtac ttatgcctga tcgttcctct aattctatgg agacggcaat taataacctt
      721 atcaaacatc tacctaaagg tgccgttaaa actattactg tcgatagagg gaaagagttc
      781 tcttgttatc aaaatattga aaaccaattt aatattaatg tctacttcgc tgatccatat
      841 tccgcatggc aaagaggtac aaatgaaaat accaatggtt
//
LOCUS       DAMZPP010000251          875 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMZPP010000251 DAMZPP010000000
VERSION     DAMZPP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.255"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(172..594)
                     /locus_tag="POH73_002803"
     CDS             complement(172..594)
                     /locus_tag="POH73_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDI6888696.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDPDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            742..>875
                     /locus_tag="POH73_002804"
     CDS             742..>875
                     /locus_tag="POH73_002804"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="catalyzes the dehydration of 3-dehydroquinate to
                     form 3-dehydroshikimate in aromatic amino acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate dehydratase"
                     /protein_id="HDI6888697.1"
                     /translation="MTHVEVVATITPQLYIEETLIQKINHRIDAIDVLELRIDQFENV
                     T"
BASE COUNT          290 a          142 c          135 g          308 t
ORIGIN      
        1 tattggaacc ggtcacattt atgatgacta agggacaatc attttcagtt aaatattgcc
       61 agaaatttca atcacgatta gaaaaaataa ccaaccaaaa atttttggtt ggcaaatttg
      121 aattttatgt gaattttatt aataacgttg aataatctct ttaagttaat gctaatctac
      181 aacatttaca tttaaatcga cattaatatt tccttgagtc gcttttgaat atggacaaaa
      241 ttcatgagcc atttgtaaat atttttcagc ttcttcttga gatataacat ttttaattgt
      301 cgcatcaatt gaaacactta atttaggact ttctgagtct ggatcatctt ctagtctcac
      361 tgttagtgtt acttctggat gagcatcacg cactttgttt tgctttaaaa ttaggtcgaa
      421 ggcaccgttg aagcaagatg cataacctgc tgcaaataat tgttcggggt tagtagcttt
      481 accatctgct tgagcaggcg gaacgatatc aatatctaat gctcgatcat cagtataaac
      541 atgtccttta cgtccgccaa cattcgtcgc tttagtttca taatgtattg ccattcttat
      601 aacctcctaa tttaaaatac atttatgctt tacccattcg aaaatacctt aatcatattc
      661 atttatagca ttgtttgatt gaaggataaa aagttgttgt ttacaataaa aataatgagt
      721 atctgaaatg agggatttac tatgacacat gtggaagtag tagcgactat cacgccacaa
      781 ttatatatcg aagaaacttt aattcaaaaa attaatcatc gtattgatgc aatagacgta
      841 ttagaattac gaattgatca atttgaaaat gtcac
//
LOCUS       DAMZPP010000252          868 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMZPP010000252 DAMZPP010000000
VERSION     DAMZPP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.256"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            242..355
                     /locus_tag="POH73_002805"
     CDS             242..355
                     /locus_tag="POH73_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888698.1"
                     /translation="MNGILIKCLNIGGQYYLQIRMLSNEDNAEKTISGNSI"
BASE COUNT          350 a           80 c          141 g          297 t
ORIGIN      
        1 ttttccaatg atttacacat taaaatgttt gaaaattgtc acaattcagt ctaattttaa
       61 aggaaattaa aattaaatta attacaaaat tcaaaaatca tacatcgtgt catttggata
      121 ttgaaaaaga tgagtcaaat ttatttcata tattttccaa taacaaatga ttatatctaa
      181 atatacaatc agaaatatat gtatagaaat atcaatatga aaaataatat gattaaaaat
      241 aatgaatggc atacttataa agtgtctaaa tattggtggt caatattact tgcaaatacg
      301 aatgttaagt aatgaagaca atgctgaaaa aacaataagt ggaaactcta tatagaatgt
      361 tcgtttttat ttttaataat tgaataatca ttattgatga tttaatatgt attcggtcgc
      421 acacgataca aagtgacttg ttttaatgat atgcaataat atgacgttta tcgtcgcgtg
      481 taacaatgta tgagtgtgtc agtttaattt taaaagggtt ataaggatat ttaataagat
      541 aaaggaggga ttaataaaca atcacatgtg aagtttttaa ggattaaatg agttgggcag
      601 ctctttaatc catagttata acgaatttta aattgttaga tttaacgtat ggtatcagta
      661 tgtatgagtt taacttaagt attaatctat agtttaaaga gcggtctaat tgtcttttta
      721 aaaatagaaa ataaggatga ttataatgag gggttataac gaaagatatt gtagctggac
      781 taatacagct atacaatgtg attattttaa taaagtaaga caggtgaaaa aagacggtaa
      841 atcggatagg ttgagcaaca acataaac
//
LOCUS       DAMZPP010000253          857 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMZPP010000253 DAMZPP010000000
VERSION     DAMZPP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.257"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>857
                     /locus_tag="POH73_002806"
     CDS             <1..>857
                     /locus_tag="POH73_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDI6888699.1"
                     /translation="KGYEIEHHQCGVALYQLIPSKKEGEPDKKVFITNTIPQITERFE
                     DIESNEVSFNMLFYDNKTPVNIAVSAEEISDSRQLLKLVNKKLDVTSSTSTKLVDYIN
                     ISKRYNPPLNVKVATRLGHVKGYFIYPYQEVMKDSNVKLFSNDKGFQKLIDSFRSKGT
                     LQGYSKKVFAQIKDLPMVMVMLYASLGSVLLREFGLQPFIVEISGSTSTGKTFTLNLV
                     SSVWGTSDLITTWSSTQNSIESMASFLNSFPMFKDDTRNTHPKFVASATYNFSSGESK
                     SRSNINLTL"
BASE COUNT          309 a          133 c          150 g          265 t
ORIGIN      
        1 aaaggctatg aaattgaaca tcatcaatgc ggtgttgcct tatatcaact tataccaagt
       61 aaaaaagaag gcgaaccaga taaaaaggtt tttatcacaa atacaatccc tcaaattact
      121 gaacgctttg aagatattga gagtaacgaa gtcagcttta atatgctttt ctatgacaat
      181 aaaacgccag taaatatagc tgtgagtgcc gaagaaattt cagatagtcg tcaactcttg
      241 aaattggtta ataaaaagct agatgtaaca tcgtcaacat ctactaaact tgttgactat
      301 attaatatat ctaaacggta taatccacca ttaaatgtta aagttgcaac gcgtttgggg
      361 catgtgaaag gttattttat ttatccttat caagaagtaa tgaaagacag caatgtcaag
      421 ttgtttagca atgataaagg gtttcaaaag ttaatagact cttttcgaag taaaggaaca
      481 ctacaaggtt actctaaaaa ggtgtttgct caaataaaag atttaccaat ggtaatggtt
      541 atgttgtatg catctttagg ctcagtttta ttaagagaat ttggattaca accctttatt
      601 gtagaaatat caggtagtac atccacaggt aaaacattca cactcaactt agtatcaagt
      661 gtttggggaa ccagtgacct tattacgaca tggagttcta ctcaaaatag tattgaatca
      721 atggcgtcat ttttgaactc atttccaatg tttaaagatg atacgcgtaa cacacatcct
      781 aagtttgttg ccagtgccac atataacttt tctagtggtg aaagtaaatc gagaagtaat
      841 attaatttaa cgctaaa
//
LOCUS       DAMZPP010000254          857 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMZPP010000254 DAMZPP010000000
VERSION     DAMZPP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.258"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>857
                     /locus_tag="POH73_002807"
     CDS             <1..>857
                     /locus_tag="POH73_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDI6888700.1"
                     /translation="KGYEIEHHQCGIALNQLIPSKKEGEPDKKVFITSTIPQITERFE
                     DIESNEVSFNMLFYDNKTPVNIAVSAEEISDSRQLLKLVNKKLDVTSSTSTKLVDYIN
                     ASKRYNPPLNVKVATRLGHVKGYFIYPYQEVMKDSNIKLFSNDKGFQKLIDSFQSKGT
                     LEGYSKKVFGQIKDLPMVMVMLYASLGSVLLREFGLQPFIVEISGSTSTGKTFTLNLV
                     SSVWGTSDLITTWSSTQNSIESMASFLNSFPMFKDDTRNTHPKFVTSATYNFSSGESK
                     SRSNINLTL"
BASE COUNT          311 a          131 c          151 g          264 t
ORIGIN      
        1 aaaggttatg aaattgaaca tcatcaatgc ggtattgcct taaatcaact tataccaagt
       61 aaaaaagaag gcgagccaga taaaaaggtt tttatcacaa gtacaatccc tcaaatcact
      121 gaacgctttg aagatattga gagtaacgaa gtcagcttta atatgctttt ctatgacaat
      181 aaaacgccag taaatatagc tgtgagtgcc gaagaaattt cagatagtcg tcaactcttg
      241 aaattggtta ataaaaagct ggatgtaaca tcgtcgacat ctactaaact tgttgattat
      301 attaatgcat ctaaacggta taatccacca ttgaatgtta aagttgcaac gcgtttgggg
      361 catgtgaaag gttattttat ttatccttat caagaagtga tgaaagacag caatatcaag
      421 ttgtttagta atgataaagg atttcaaaag ttaatagact cttttcaaag caaaggaaca
      481 ttagaaggtt actctaaaaa agtgttcggt caaataaaag atctaccaat ggtaatggtt
      541 atgttatatg cctctttagg ttcggtttta ttaagagaat ttggattaca gccctttatt
      601 gtagaaatat caggtagtac atctacaggt aaaacattca cactcaactt agtatcaagt
      661 gtttggggaa cgagcgacct tatcacgaca tggagttcta ctcaaaatag tattgaatca
      721 atggcatcat ttttgaactc atttccaatg tttaaagatg atacacgtaa tacacatcct
      781 aagtttgtta ccagtgccac atataacttt tctagtggtg aaagtaaatc aagaagtaat
      841 attaatttaa cactaaa
//
LOCUS       DAMZPP010000255          815 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMZPP010000255 DAMZPP010000000
VERSION     DAMZPP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.259"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..488)
                     /locus_tag="POH73_002808"
     CDS             complement(<1..488)
                     /locus_tag="POH73_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDI6888701.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EG"
     gene            complement(504..>815)
                     /locus_tag="POH73_002809"
     CDS             complement(504..>815)
                     /locus_tag="POH73_002809"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="E3 component of the branched-chain alpha-keto acid
                     dehydrogenase complex; catalyzes the oxidation of
                     dihydrolipoamide to lipoamide; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDI6888702.1"
                     /translation="QAKAEGMKVKSFKVPFKAIGKAVIDSHDANEGYSEMVIDQSTEE
                     IVGINMIGPHVTELINEASLLQFMNGSALELGLTTHAHPSISEVLMELGLKAESRAIH
                     V"
BASE COUNT          224 a          189 c          116 g          286 t
ORIGIN      
        1 aaaccttcat ggaagtcacc ctggtttgaa ctaccttcac caacagttgc tgttgcaatt
       61 ttcttcttac catccatttt tagagctaaa gcagcaccaa cagcatgggg tatttgagtt
      121 gctaccggtg aactttgtga caaaatattc ttagctctac tactaaagtg tgatggcatt
      181 tgttttccac cagagttaac atcgtctttc tttccaaacg ctgataaaaa cgtatcatac
      241 gctgatatac ccatataagt aacgaaagct agatctctat aataaggcgc agtaatatca
      301 ccttcttcta atgcgtatgc catcccaatc tgagttgctt cttgtccttg accacttaca
      361 acaaatggaa ttttacctgc acggttcaat aaccacagtc tttcatctat ttttctacct
      421 aaatccatcc atttatatat tacttttagg tcttcttcgc taaggcctaa tgatttataa
      481 tcaatcatgt taaatcctcc tatttatacg tgaatagctc tactttctgc tttcaatcct
      541 aattccatca acacttcaga gatggaagga tgtgcgtgtg ttgttagtcc taattctaat
      601 gccgagccat tcatgaactg taacagtgat gcctcattaa tcaattctgt tacatgtgga
      661 ccaatcatat taatacccac aatttcttca gttgattgat caatcaccat ttcgctatac
      721 ccttcgtttg cgtcatggct atcaatcact gctttaccaa ttgctttaaa tggtacttta
      781 aaacttttaa ctttcattcc ctctgccttt gcttg
//
LOCUS       DAMZPP010000256          708 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMZPP010000256 DAMZPP010000000
VERSION     DAMZPP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.260"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..211
                     /locus_tag="POH73_002810"
     CDS             <1..211
                     /locus_tag="POH73_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888703.1"
                     /translation="QSQQNEKTFSQEEVSQLIKERIARERKKSDERIKDAKENNDSNE
                     VAYLLKGGKVTKVDGNQNSVSFVPG"
     gene            224..523
                     /locus_tag="POH73_002811"
     CDS             224..523
                     /locus_tag="POH73_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888704.1"
                     /translation="MLFDSKPNSIVMLHNYPGQSGFSEYDLFTFFKHPSIKSMTIVTN
                     KEQVKFITKSDRFQGKIVSKFCTKYFTHINIINDSYIEKLLKKLYSINMIKYKVR"
     gene            520..657
                     /locus_tag="POH73_002812"
     CDS             520..657
                     /locus_tag="POH73_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888705.1"
                     /translation="MILIMIDSQLDGNVTAKELSESFKELVEEFERIEKANKNSNSKD
                     K"
BASE COUNT          298 a           82 c          113 g          215 t
ORIGIN      
        1 acaatcacaa cagaatgaga aaacattttc tcaagaagaa gtatcacaat tgattaaaga
       61 gcgtatagct agagaacgca aaaaatcaga tgaacgtatt aaagatgcca aagaaaacaa
      121 tgatagcaat gaagtagctt atttattaaa aggtggtaaa gttacaaaag tagatggcaa
      181 tcaaaatagt gtatcttttg tgccagggta acaagcaacc gaattgttat ttgacagtaa
      241 accgaattca attgttatgt tacataacta tcctggccaa tcaggatttt cagaatatga
      301 tttatttaca ttttttaagc atccatcaat aaaatcaatg acaatcgtta ctaataagga
      361 acaagtaaaa tttattacca agtcagatag gtttcaaggt aaaatagtga gtaaattttg
      421 tactaaatat tttacgcata ttaatatcat taatgatagc tatattgaaa aattattaaa
      481 aaaactttat agtataaata tgattaaata taaagtgaga tgattttaat tatgattgat
      541 tcacaattag atggaaatgt aactgcaaaa gaattgagtg aatcttttaa agaattagtt
      601 gaagaatttg aacgcataga aaaagcgaat aagaattcaa attcaaaaga taaatagcgt
      661 cctttctaca cagataaaga gaaagcggtg ctgtttttat acactttt
//
LOCUS       DAMZPP010000257          667 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMZPP010000257 DAMZPP010000000
VERSION     DAMZPP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.261"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..194
                     /locus_tag="POH73_002813"
     CDS             <1..194
                     /locus_tag="POH73_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI6888706.1"
                     /translation="MFIYFTVVPYYITKYIVKLKQDGKKLFFFLWDFVIFVFNVQLRK
                     VKLSYDEEVEYHDKKITFK"
     gene            229..>667
                     /locus_tag="POH73_002814"
     CDS             229..>667
                     /locus_tag="POH73_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6888707.1"
                     /translation="MNLKAPYAGLRDNLLLTKTGEVWAYYRVRSESISTANYDAKEES
                     KKRMRYFLESIKGYQDFHFEMYDHDLDLRNKFKEISKDFDDEAFNVAEYYSKETIDVL
                     EQELQMITHNSFVMGVKIRELADDAVTLKEILKSTLSDTAERIV"
BASE COUNT          238 a           63 c          123 g          243 t
ORIGIN      
        1 ttatgtttat ttactttacg gttgtccctt attatataac gaaatatatt gttaagttaa
       61 agcaagatgg taagaaatta tttttcttct tatgggattt tgtaatattt gtttttaatg
      121 ttcagttaag aaaagttaaa ttaagttatg atgaagaagt agaatatcat gataaaaaaa
      181 taacatttaa atagccccta ttggggttat ttttattgga gggatgacat gaatttaaaa
      241 gcaccgtatg caggtttaag agataatttg ttattaacga aaactggcga agtttgggcg
      301 tattatcgtg ttcgttctga gtctatttca acagcaaatt atgatgcaaa agaagaatca
      361 aaaaagcgta tgcgttattt tttagagtcg attaaaggtt atcaagattt tcattttgaa
      421 atgtatgacc atgatttaga tttgcgaaat aaatttaaag aaattagtaa agattttgat
      481 gatgaagctt ttaatgtagc tgaatattat tcaaaagaaa ctattgacgt attagaacaa
      541 gaattacaaa tgattacgca taattctttt gttatgggtg ttaaaatacg tgaattagct
      601 gacgatgcag ttacacttaa agagatttta aaaagtacat tatcagatac ggctgagcgt
      661 attgttt
//
LOCUS       DAMZPP010000258          557 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMZPP010000258 DAMZPP010000000
VERSION     DAMZPP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.262"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..515)
                     /locus_tag="POH73_002815"
     CDS             complement(<1..515)
                     /locus_tag="POH73_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDI6888708.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALV"
BASE COUNT          193 a          115 c           88 g          161 t
ORIGIN      
        1 actaaagcga ttgctgtaac tgtaatgcca gacactagcg ctggcatggc ttcaggtaag
       61 aggactttac gaattactgt ccaagtatta gcacccattg accaggccgc ttcgatgaca
      121 cctttatcaa tttctttaaa agcaatttct acgagccttg cataaaacgg cgctgcgcca
      181 atgatcaatg ctggtaacgc acctgtcgga ccacttatcg ttccaagtat caaacttgta
      241 aatggaatta ataataaaat taaaataata aatggtatcg ctctaaataa gttaacaatg
      301 aaagaaacga tagaataaaa taaccttgca ccaatagact tacctttagc agacaagaat
      361 aataacacac ctaaaataag accgagtata aatgcaaata tagttgagac gactgtcatg
      421 tatagtgttt cgactattgc agtccaaact tctggccact gaatattagg cattgtaatc
      481 atttcattta taatttcact aaatgattta cccatgtctt aacacctcca ttttaacttg
      541 tcgctcaatt aactctt
//
LOCUS       DAMZPP010000259          487 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMZPP010000259 DAMZPP010000000
VERSION     DAMZPP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.263"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>487
                     /locus_tag="POH73_002816"
     CDS             <1..>487
                     /locus_tag="POH73_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HDI6888709.1"
                     /translation="ANEPGIKDVKVTLKDSTGKVIGTTTTDASGKYNFTDLDNGNYTV
                     EFETPAGYTPTVKNTTAEDKDSNGLTTTGVIKDADNWTLDSGFYKTPKYSLGDYVWYD
                     SNKDGKQDSTEKGIKDVTVTLQNEKGEVIGTTKTDENGKYHFDNLDSGKYKVIFEKPA
                     GL"
BASE COUNT          197 a           63 c           95 g          132 t
ORIGIN      
        1 gctaatgaac ctggtatcaa agatgttaag gttacattaa aagatagtac tggaaaagtt
       61 attggtacaa ctactactga tgcctcgggt aaatataatt ttacagattt agataatggt
      121 aactatacag tagaatttga aacaccagca ggttacacgc caacggttaa aaacactaca
      181 gctgaagata aagattctaa tggtttaaca acaacaggtg ttattaaaga tgcagataat
      241 tggacattag acagtggttt ctataaaaca ccaaaatata gtttaggtga ttatgtttgg
      301 tacgacagta ataaagacgg caaacaagat tcaactgaaa agggtattaa agatgtgaca
      361 gttacattgc aaaacgaaaa aggcgaagta attggaacaa ctaaaacaga tgaaaatggt
      421 aaatatcatt tcgataattt agatagcggt aaatacaaag ttatttttga aaagcctgct
      481 ggcttaa
//
LOCUS       DAMZPP010000260          452 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMZPP010000260 DAMZPP010000000
VERSION     DAMZPP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.264"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>452
                     /locus_tag="POH73_002817"
     CDS             <1..>452
                     /locus_tag="POH73_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HDI6888710.1"
                     /translation="PNDIPMMPDEIKYEDYRESLSLPDIVANGALPIGLDYEGVTLQK
                     IKLTEPAMISSENPREIAHIAEIMMKEIDILNEKYAICIADSSGEFKAYRHQVANFAE
                     EREDIKAIHQLMIEDLKQREMDGPFEKDSLYIINDFKTYIDCTYIPED"
BASE COUNT          181 a           64 c           82 g          125 t
ORIGIN      
        1 ccaaatgata ttcctatgat gccagatgaa attaaatatg aagattacag agaatcatta
       61 agcttaccag atatagttgc caatggtgct ttaccaattg gattagatta tgaaggtgtt
      121 acactacaaa aaattaaatt aactgaacca gcaatgattt catcagaaaa tccgagagaa
      181 attgcgcata ttgccgaaat tatgatgaaa gaaattgaca tattaaatga aaaatatgcg
      241 atttgtatcg cagactcaag tggagagttt aaagcttata ggcatcaagt ggctaacttt
      301 gccgaagaaa gagaagacat taaagcgatt catcaactaa tgattgaaga cttaaaacaa
      361 agagaaatgg acggtccatt tgaaaaagat tcactttaca ttatcaatga ttttaaaaca
      421 tatattgatt gcacgtatat cccggaagat ga
//
LOCUS       DAMZPP010000261          439 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMZPP010000261 DAMZPP010000000
VERSION     DAMZPP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.266"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..429
                     /locus_tag="POH73_002818"
     CDS             <1..429
                     /locus_tag="POH73_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDI6888711.1"
                     /translation="VLVLMADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFY
                     SHYLFASSTAHISAMYAALLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPIL
                     FSSGYVTQKRWWTMNLILGFVYFIIWIGLGSLWMKVIGIF"
BASE COUNT          116 a           79 c           85 g          159 t
ORIGIN      
        1 gtgttagtgt taatggccga ccaattgaac aagcttggat ttattccttg gttaagtaaa
       61 tccattgcta caagtcttgg tggattaagc tggcctatag tactggtcat tttaatattg
      121 ttctacttct attcacatta cttatttgca agttctacag cacatatcag tgctatgtat
      181 gcagcattac taggcgttgc catcgcagcc ggtgcaccac cattattcag tgcattaatg
      241 ttaggtttct ttggtaacct attagcttca acaacacact atagtagtgg tccagcgccg
      301 attctcttct cttcaggtta cgtgactcaa aaacgttggt ggacaatgaa cttaatatta
      361 ggtttcgtct actttattat ctggattggt ttaggatcac tttggatgaa agtaattggt
      421 atattttaaa atgtttaaa
//
LOCUS       DAMZPP010000262          426 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMZPP010000262 DAMZPP010000000
VERSION     DAMZPP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.267"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            83..331
                     /locus_tag="POH73_002819"
     CDS             83..331
                     /locus_tag="POH73_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888712.1"
                     /translation="MLILKIKEINDKSVTYKYFPNNDENIKPGIIQMDIDSLEVINAE
                     KSSLEKNTRDNYFIHAIDRIYINTSKGLFPESELVAWG"
BASE COUNT          179 a           44 c           60 g          143 t
ORIGIN      
        1 taatttacac tatatagagg cacataaact tacagaaata aaatataatt atagtatttt
       61 aattaattag aggtggaaat gattgctaat attaaaaata aaagaaatta atgataaaag
      121 tgttacatat aaatattttc ctaataatga tgaaaatatt aagccgggta ttattcaaat
      181 ggatatagat agccttgaag ttatcaatgc tgaaaaatct agtttagaaa aaaatacaag
      241 agacaattat tttattcatg ccatagacag aatatacata aatactagta aagggctatt
      301 tcctgaatct gaactcgtag catggggata aagaaagttt acttttaaaa atttcatatt
      361 taattttaaa aattttaacc ttccaatgtg aaggttattt ttattgtcca aaacgtgctg
      421 atgaca
//
LOCUS       DAMZPP010000263          418 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMZPP010000263 DAMZPP010000000
VERSION     DAMZPP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.268"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..126
                     /locus_tag="POH73_002820"
     CDS             <1..126
                     /locus_tag="POH73_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDI6888713.1"
                     /translation="TDGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            138..>418
                     /locus_tag="POH73_002821"
     CDS             138..>418
                     /locus_tag="POH73_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6888714.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLV"
BASE COUNT          155 a           52 c           91 g          120 t
ORIGIN      
        1 acagacggtt taaacgctat attaggattt ttagtattaa ctggtgtgat tcaagacccg
       61 actactaaag gtataggtga tagccaccaa gctttagaat atgaagaacc aagaagaaaa
      121 tactaggagg taaaataatg aaaacataca gtgaagcaag agcaaggtta cgttggtatc
      181 aaggtagata tattgatttt gacggttggt atggttacca atgtgcagat ttagcagttg
      241 attacattta ttggttgtta gaaattagaa tgtggggaaa tgcaaaagat gcaatcaata
      301 acgattttaa aaacatggca acagtatatg aaaacacacc atcgtttgtt ccacaaatag
      361 gtgatgtggc tgtatttacc aaaggaatat ataaacaata cggtcatatt ggtttagt
//
LOCUS       DAMZPP010000264          394 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMZPP010000264 DAMZPP010000000
VERSION     DAMZPP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.269"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>394
                     /locus_tag="POH73_002822"
     CDS             <1..>394
                     /locus_tag="POH73_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDI6888715.1"
                     /translation="PALEFATSTSIANALMDMVVQGLNPAKNQGYFIMYGDKVQFQRS
                     YHGTMAVTKRVAGAEEINAEVIFEGDEVKYKTKNGKIVELEHTQSFGNRNTQNIIGAY
                     ATVVFKDESRNYTEIMTFEEIEEAWKQS"
BASE COUNT          155 a           63 c           81 g           95 t
ORIGIN      
        1 aaccagcgct ggaatttgca acttcaacca gcatagcaaa cgccttaatg gacatggttg
       61 tacaaggttt aaatcctgct aagaatcaag gctatttcat tatgtatggc gataaggttc
      121 aattccaaag aagttaccac ggaacaatgg cagtaactaa acgtgtagca ggcgcagaag
      181 aaattaatgc agaagtcata tttgaaggtg acgaagttaa gtataaaact aaaaacggaa
      241 aaattgttga acttgaacat acacagtctt ttggtaacag aaacacacaa aacattatcg
      301 gtgcatatgc aacagttgta tttaaagatg aaagtagaaa ttacactgaa atcatgacat
      361 ttgaagagat tgaagaagcg tggaagcaat caca
//
LOCUS       DAMZPP010000265          390 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMZPP010000265 DAMZPP010000000
VERSION     DAMZPP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.270"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..371
                     /locus_tag="POH73_002823"
     CDS             <1..371
                     /locus_tag="POH73_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HDI6888716.1"
                     /translation="VRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMAFIVFTII
                     MITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFHALFITYL
                     FGMTTYFIMLMLRSVRSDKQ"
BASE COUNT          122 a           68 c           65 g          135 t
ORIGIN      
        1 tagtgcgaca tgcggatgca agtttagcat atggtggttg gccattgcca ttccacgatc
       61 ttgtaccaca ttcagaacaa gattgggtcc aactcacgca tcgtatcatg gcttttatag
      121 tgtttacgat tattatgatt acttatattc acgctgttaa aaattatcca aataaccgta
      181 ctgttcatta tggttataca gctgcattta tacttgttat attacaagtt atcacaggtg
      241 cattgtctat tatgactaat gttaatttga taatcgcact attccatgca ttatttatca
      301 cttatttatt tggtatgaca acatacttta tcatgctaat gttacgatca gtaagaagtg
      361 acaagcaata acaaaaaagc aaagaagctg
//
LOCUS       DAMZPP010000266          357 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMZPP010000266 DAMZPP010000000
VERSION     DAMZPP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.271"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(249..>357)
                     /locus_tag="POH73_002824"
     CDS             complement(249..>357)
                     /locus_tag="POH73_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDI6888717.1"
                     /translation="LIGFEHPVTGEYIERHAELAQDFEDLLDTIRKRDA"
BASE COUNT          119 a           57 c           42 g          139 t
ORIGIN      
        1 atatctatat tttcatgtct ttgacgtctc tctggacgct caattaaaga ctctttatgt
       61 tgtacttaaa ctgttataag gataatacta atcaggcgct ttgtcaatct atattgaaaa
      121 attaaccatt ctcagtaaaa atgttaacct taatctattt tataatgtgt tttcattttt
      181 aaaaaaacga aaacgataaa tacttatagt ttacataata atgttatcgt aattatagca
      241 cgacacaatt atgcatctct ttttcgaatt gtatctaata aatcttcaaa gtcttgtgct
      301 aattcagcat gtctttcaat atattcacct gttactggat gttcgaatcc aataagt
//
LOCUS       DAMZPP010000267          347 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMZPP010000267 DAMZPP010000000
VERSION     DAMZPP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.272"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..107
                     /gene="cstR"
                     /locus_tag="POH73_002825"
     CDS             <1..107
                     /gene="cstR"
                     /locus_tag="POH73_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HDI6888718.1"
                     /translation="ISENLIECVKVAADDEESSQELINEAVNLLVKSK"
     gene            107..>347
                     /locus_tag="POH73_002826"
     CDS             107..>347
                     /locus_tag="POH73_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDI6888719.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQ"
BASE COUNT          108 a           54 c           70 g          115 t
ORIGIN      
        1 ttattagtga gaatttaata gaatgtgtaa aagtagctgc ggatgatgaa gaaagctccc
       61 aagagttaat taatgaagct gtaaacttat tggtgaaaag taagtaatgg atattgttaa
      121 tatagttatc atgttactca ttggggtatt cggtgggttc atatctggtc tagtaggcgt
      181 aggtggtgct atcattattt atccagctat tttattatta ccaccattat tcggagcccc
      241 tgcatatagc gcatatatcg catcaggtct tacttctagc caagtctttt tcagtacatt
      301 aagtggctca ttaaaagctc gaaaaaaaac agagttctca ccacaat
//
LOCUS       DAMZPP010000268          337 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMZPP010000268 DAMZPP010000000
VERSION     DAMZPP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.273"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>337
                     /locus_tag="POH73_002827"
     CDS             <1..>337
                     /locus_tag="POH73_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDI6888720.1"
                     /translation="KLQPGEERVKQEGQPGSKTITTPITVNPLTGEKVGEGQPTEEIT
                     KQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSGPVNPNNPGLSKDRAKPNGPVHSM
                     DKNDKVKKS"
BASE COUNT          150 a           69 c           69 g           49 t
ORIGIN      
        1 caaaattaca acctggtgaa gagcgagtga aacaagaagg acaaccagga agtaagacaa
       61 tcacaacacc aatcacagtg aacccattaa caggtgaaaa agttggcgag ggtcaaccaa
      121 cagaagagat cacaaaacaa ccagtagata agattgtaga gttcggtgga gagaaaccaa
      181 aagatccaaa aggacctgaa aacccagaga agccgagcag accaactcat ccaagtggcc
      241 cagtaaatcc taacaatcca ggattatcga aagacagagc aaaaccaaat ggcccagttc
      301 attcaatgga taaaaatgat aaagttaaaa aatctaa
//
LOCUS       DAMZPP010000269          335 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMZPP010000269 DAMZPP010000000
VERSION     DAMZPP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.274"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>335
                     /locus_tag="POH73_002828"
     CDS             <1..>335
                     /locus_tag="POH73_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6888721.1"
                     /translation="RNRIIPTKPITDEKLKKEIENFKFFVQYGNFKDFKDYKDGDISY
                     NPNVPSYSAKYQLNNDDYNVQQLRKRYDISTKRAPELKLRGSGDLKGSSVGSKELEFN
                     FVRNKEENV"
BASE COUNT          140 a           43 c           60 g           92 t
ORIGIN      
        1 cgtaatcgaa taattccaac gaagcccata acggacgaga agttaaaaaa agagattgaa
       61 aactttaagt tctttgtaca atatgggaat tttaaagatt ttaaagatta taaagatggg
      121 gatatttcat acaatcctaa cgtaccaagt tattccgcaa agtatcaatt gaataatgat
      181 gattataatg tccaacaatt aagaaaacga tatgatatat caaccaaaag ggcacctgaa
      241 ttaaaattga gaggatctgg cgatttaaaa ggctcatccg taggttctaa agaactagaa
      301 tttaactttg taagaaataa agaggaaaat gtgta
//
LOCUS       DAMZPP010000270          331 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMZPP010000270 DAMZPP010000000
VERSION     DAMZPP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.275"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>331
                     /locus_tag="POH73_002829"
     CDS             <1..>331
                     /locus_tag="POH73_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001667297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mobile element-associated protein"
                     /protein_id="HDI6888722.1"
                     /translation="KVLANKIGHKVDEGSYQPSRCFALPVIQKGHIFIKRVNDCPIMD
                     VDMLEQWLKEYEQSNVSPSVIGYTRRDSKYWRELCFGTTEGNRNNALASLVGHLLRCH
                     VNDYIVYS"
BASE COUNT          113 a           45 c           74 g           99 t
ORIGIN      
        1 caaaggtatt agcaaataaa attggtcata aagtggatga aggttcatat cagccaagta
       61 gatgttttgc actaccagtt attcaaaaag gacacatatt tattaaacga gtgaatgact
      121 gtccaattat ggatgttgat atgcttgaac agtggttaaa ggagtatgaa caatcaaatg
      181 ttagtccgag tgtcatagga tacacgcgac gagatagtaa gtattggcga gagttatgct
      241 ttggaacaac cgaaggcaat cgtaacaatg cactagctag cttagttggg catttattaa
      301 gatgtcacgt taatgattat attgtttatt c
//
LOCUS       DAMZPP010000271          321 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMZPP010000271 DAMZPP010000000
VERSION     DAMZPP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.276"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            160..>321
                     /locus_tag="POH73_002830"
     CDS             160..>321
                     /locus_tag="POH73_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6888723.1"
                     /translation="MKNNLKIARVSLSMTQKELANKVGVTRQTISLIEKGVHNPSLSL
                     CKNICSVLNK"
BASE COUNT          124 a           37 c           49 g          111 t
ORIGIN      
        1 agtaatgagt gtagtatata ttttaacttc agtcataatg ttctacatta atggattaag
       61 taaacaattt tttacatatg tagttggttt cccaattatt tattttggtt atatatgtct
      121 agttaatctt ataaaaaaaa gaagccggaa taattaaaag tgaaaaataa tttgaaaata
      181 gctagagttt cactatctat gactcaaaag gagctagcaa ataaagttgg agtaactaga
      241 caaacaatat ctcttataga aaagggtgta cataacccct ctctatcact atgtaaaaat
      301 atttgttcgg tgttgaataa a
//
LOCUS       DAMZPP010000272          317 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMZPP010000272 DAMZPP010000000
VERSION     DAMZPP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.277"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>317
                     /locus_tag="POH73_002831"
     CDS             <1..>317
                     /locus_tag="POH73_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6888724.1"
                     /translation="YPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLK
                     EDRMLLKVDKNTHKITGEYDTTTNDRKDATDSTYKSYPVKVVNNKIVFTKDVKDPALK
                     QKI"
BASE COUNT          133 a           50 c           52 g           82 t
ORIGIN      
        1 gtatcctatt aaaaacttag aaacgcttta tgaaattgat ggtcacgata atccccatta
       61 tgaaaataat gatcaagaca catggtatat agaatcctct tattcagttg ttggatcaga
      121 tgagctttta aaagaagacc gtatgttgtt aaaggtagat aagaacacac ataaaataac
      181 tggtgaatat gacacaacta caaacgatag aaaagatgct actgactcga catacaaaag
      241 ttatccagta aaagtagtta ataataaaat cgttttcaca aaagacgtaa aagatcccgc
      301 gctgaaacaa aaaatcg
//
LOCUS       DAMZPP010000273          316 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMZPP010000273 DAMZPP010000000
VERSION     DAMZPP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.278"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            57..>316
                     /locus_tag="POH73_002832"
     CDS             57..>316
                     /locus_tag="POH73_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HDI6888725.1"
                     /translation="MGEFEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSKLDSKR
                     LYNAKVSFANPTDLENKKTNDRLLKHDLLFHDMFLNDAWKKD"
BASE COUNT          111 a           39 c           50 g          116 t
ORIGIN      
        1 tttcctagcc tgtttatcat ttatgtctcg ctctcaaatg taaattaata attgttttgg
       61 gggagtttga agtaaaatat ttaacaggat ttattttaat attattgtta gaaggaattt
      121 ttacaaattc agcaagtgca atcgaatatt cagacttaca tcataaaagt aaacttgatt
      181 ccaagcgtct atataatgct aaggtgtcat ttgctaatcc aactgatctt gaaaataaaa
      241 aaacaaatga tagactgttg aagcatgatt tgttatttca tgacatgttc ttaaatgatg
      301 cttggaaaaa agattt
//
LOCUS       DAMZPP010000274          309 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMZPP010000274 DAMZPP010000000
VERSION     DAMZPP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.279"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            11..>309
                     /locus_tag="POH73_002833"
     CDS             11..>309
                     /locus_tag="POH73_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012990921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDI6888726.1"
                     /translation="MNIETIVNEFETRAGTLLRYYTGLLEHSKVQPCCFKLYNDPFDM
                     VYVVMNNKLFGHVYIKDCKVRKSFELASPKHTEGLIRSIEGHYVGYELHDGKQLS"
BASE COUNT          110 a           39 c           69 g           91 t
ORIGIN      
        1 agaaaatcaa atgaacatag aaactatcgt aaatgaattt gagacgcgag caggcacgct
       61 attaaggtac tacacgggat tattagaaca tagtaaagta caaccatgtt gctttaagtt
      121 atataatgat ccatttgata tggtttatgt ggtgatgaac aacaagttat tcggtcatgt
      181 atatattaaa gattgtaagg tgaggaagtc atttgaatta gcgtcaccta agcacactga
      241 ggggcttata agaagcatag aggggcatta tgtaggttat gaattacatg atggtaaaca
      301 gctttctat
//
LOCUS       DAMZPP010000275          297 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMZPP010000275 DAMZPP010000000
VERSION     DAMZPP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.280"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
BASE COUNT          131 a           27 c           47 g           92 t
ORIGIN      
        1 ggttctttta ttgaatcggg tgcacttaat aataaaagtg atgaatacgg tatcaaaagg
       61 aacaggcatg ctcaaattta ttataattca gttagaaatc aagaaaacag atagagattt
      121 ctaaaattgc taaaaatact aatataaata aaaatacaat tcaaagagtt tatgaacata
      181 tatttgaaaa taagtattta ttagaaaatg gatttaaaca atttgatcct gacttttata
      241 tggctcaaag ttggcaacgt ttaagagaag ggaaaaatat taaaaaatgg atataat
//
LOCUS       DAMZPP010000276          293 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMZPP010000276 DAMZPP010000000
VERSION     DAMZPP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.281"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(<1..>293)
                     /locus_tag="POH73_002834"
     CDS             complement(<1..>293)
                     /locus_tag="POH73_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDI6888727.1"
                     /translation="LSLMMQDIKISYDKAFIFLTLTTPNVVAERLGDEIKRYNKSFDK
                     LFKRKVVKRVVKGYVRKLEITYNEKRDDYHPHFHVLIVVNKSYFTDVKAYLNQ"
BASE COUNT           99 a           49 c           28 g          117 t
ORIGIN      
        1 tttgattcaa ataagctttt acatccgtaa aatatgattt attaacaaca ataagcacat
       61 gaaaatgtgg gtgataatca tctcgtttct cattataagt tatttctaat tttcgaacat
      121 aaccttttac tactcttttt acaacttttc gtttaaataa tttatcaaaa gatttattat
      181 atcttttaat ctcatctccc aatctttcag ctactacgtt cggtgttgta agcgtcaaaa
      241 agataaatgc tttatcataa ctaatcttta tatcctgcat cattaaagat aat
//
LOCUS       DAMZPP010000277          290 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.282, whole genome shotgun sequence.
ACCESSION   DAMZPP010000277 DAMZPP010000000
VERSION     DAMZPP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.282"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>290
                     /locus_tag="POH73_002835"
     CDS             <1..>290
                     /locus_tag="POH73_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6888728.1"
                     /translation="IANISNNATVSQADQTTIINSLTFTSNAPNRNYATASANEITSK
                     TVSNVSRTGNNANVTVTVTHQDGTTSTVTVPVKHVIPEIVAHSHYTVQGQDFP"
BASE COUNT          109 a           62 c           47 g           72 t
ORIGIN      
        1 attgccaata tttcgaataa tgcgacagta tcacaagctg atcaaacgac aattattaat
       61 tcgttaacgt ttacaagtaa tgcaccaaat agaaactatg caacagcaag cgcaaatgaa
      121 atcactagta aaacagttag taatgtcagt cgtactggaa ataatgccaa tgtcacagta
      181 actgttactc atcaagatgg aacaacatca acagtgactg tacctgtaaa gcatgtcatt
      241 ccagaaatcg ttgcacattc gcattacact gtacaaggcc aagacttccc
//
LOCUS       DAMZPP010000278          285 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMZPP010000278 DAMZPP010000000
VERSION     DAMZPP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.283"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..185
                     /locus_tag="POH73_002836"
     CDS             <1..185
                     /locus_tag="POH73_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDI6888729.1"
                     /translation="NEYFENNQLNLYQMRWNPENPAQHQYASDIRWADKIAKLMDKSY
                     KQFGIKKDDIRQTYYK"
BASE COUNT          107 a           41 c           54 g           83 t
ORIGIN      
        1 gtaatgaata ttttgaaaac aatcaactga atttatatca aatgcgatgg aatccagaaa
       61 atcctgcgca acatcaatat gcgagtgaca ttcgctgggc agataaaatt gccaaattaa
      121 tggataaatc ctataagcag tttggtataa agaaagatga tattagacaa acatattata
      181 aataagacat cggtgcttaa aggggctgga acaatttatt gtttcgagct cctttagcgc
      241 attctgagtg tgttagttaa atggatttta acctaacata aaacg
//
LOCUS       DAMZPP010000279          284 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.284, whole genome shotgun sequence.
ACCESSION   DAMZPP010000279 DAMZPP010000000
VERSION     DAMZPP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.284"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..>284
                     /locus_tag="POH73_002837"
     CDS             <1..>284
                     /locus_tag="POH73_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6888730.1"
                     /translation="QTPSYKTINRFRVNPNTDALIESLFIQFHSQCLKQNLIDDKSIF
                     IDGTKVEASANRYTFVWKKSIQNYESKLNENSKALYRDLVEEKIIPEIKE"
BASE COUNT          117 a           36 c           42 g           89 t
ORIGIN      
        1 atcaaacacc ttcttataaa actattaatc gttttagagt gaatcctaat actgatgcgc
       61 taattgaatc tttatttatt cagttccata gtcaatgttt aaagcaaaat cttattgatg
      121 ataaatcaat ttttattgat ggtacaaaag tagaagctag tgccaataga tatacatttg
      181 tgtggaagaa aagtattcaa aattacgaat cgaaattgaa cgaaaattca aaagcattat
      241 atcgtgactt agtcgaagaa aaaataatac cagagataaa agaa
//
LOCUS       DAMZPP010000280          275 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.denovo.285, whole genome shotgun sequence.
ACCESSION   DAMZPP010000280 DAMZPP010000000
VERSION     DAMZPP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.denovo.285"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(167..>275)
                     /locus_tag="POH73_002838"
     CDS             complement(167..>275)
                     /locus_tag="POH73_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDI6888731.1"
                     /translation="WNQMHYYASELRLMSEEMQKEVNHQMINELDDKYL"
BASE COUNT           86 a           63 c           24 g          102 t
ORIGIN      
        1 tcaaaaacaa attcttttac ttaattctaa aactccgcct acacatttta tattacttgt
       61 ctgaacgctc cgctggacgg ctccgccttg ctgactctgt cagcacctca gactacacta
      121 aattacatat gcaattcttt atatataaaa ttgaaaaata aagctcttat aaatatttat
      181 catctaattc atttatcatt tgatgattca cttctttttg catttcttca ctcatcaatc
      241 tcaattcact cgcataataa tgcatttgat tccaa
//
LOCUS       DAMZPP010000281         5682 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.guided.156, whole genome shotgun sequence.
ACCESSION   DAMZPP010000281 DAMZPP010000000
VERSION     DAMZPP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.guided.156"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            complement(137..517)
                     /gene="blaI"
                     /locus_tag="POH73_002839"
     CDS             complement(137..517)
                     /gene="blaI"
                     /locus_tag="POH73_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDI6888732.1"
                     /translation="MANKQVEISMAEWDVMNIIWGKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(507..2263)
                     /gene="blaR1"
                     /locus_tag="POH73_002840"
                     /pseudo
     CDS             complement(507..2263)
                     /gene="blaR1"
                     /locus_tag="POH73_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
     gene            2370..3215
                     /locus_tag="POH73_002841"
     CDS             2370..3215
                     /locus_tag="POH73_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaZ family penicillin-hydrolyzing class A
                     beta-lactamase PC1"
                     /protein_id="HDI6888733.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKALIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKENKKFLLDLMLNNKSGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(3689..4333)
                     /gene="trmB"
                     /locus_tag="POH73_002842"
     CDS             complement(3689..4333)
                     /gene="trmB"
                     /locus_tag="POH73_002842"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDI6888734.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGEMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKNGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            complement(4348..5139)
                     /locus_tag="POH73_002843"
     CDS             complement(4348..5139)
                     /locus_tag="POH73_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDI6888735.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
BASE COUNT         1974 a          875 c          716 g         2117 t
ORIGIN      
        1 aagagagtgt acagaaaatg gttattttct gtacactctc atctttaata atatatttta
       61 aatgttatta acaaaactaa tttaataaga gtcaagcata gtttacatat gtattatgtt
      121 tgaaacatat ctctaattac tttttactaa tatcatttaa aatgtctcgc aattcttcaa
      181 tttctttgtt atttaattct tcatttttcg caaaattcag cactaaactt ttcatgtccc
      241 ctccatacag tttattaaga aaggttttag cagttttcat tttaatatcg tcttctttaa
      301 tatttgatga gtaaaaataa atattctctg atttgtatcg ttttataatc tcttttttat
      361 atagtcttgt gattaatgtt ctaatcgttt tatcgctaac ttctttatat ttttgaattt
      421 caactacaat ttcattagct gatactgatt ttttacccca tattatattc ataacatccc
      481 attcagccat agatatttca acttgcttat tggccattta aaacacccat ttcttttaat
      541 attttttcac tgattaattc agcatttttc ccagatggtt ttccatctga taaatgtgta
      601 gcaaaataat acttatcatg atttgtaatt acgtaaccta caaaccaccc attattatac
      661 ttcccgttta ctatacctgt acctgttttc ccatacagtt catacttttc atttttctta
      721 atcaataatg aagaagataa ttgattcttt gcttttttac taaaatggtt attttgttcc
      781 atcatatttt taaaaactat tacttgttca agattagata ttttcaaact atcttccatc
      841 caatagcttt tataacttcc caaattttta ttaccataat ttaattgctt gagttgagtc
      901 gcagtatagt tctttggtat ttgatcgcta atacgttcga agtaccaatt aactgaattt
      961 tgcattgctg tatttaaatc ttgttcctta ttccaagcat caaaagggta atgcttatga
     1021 ttccatgaca tacgtgaatt ttcatcattt ataatatgtc ggtcaagtcc aaacatagct
     1081 aaataaattt tataagttga attaggagaa tacctttttc tactttcttt ttcgttataa
     1141 atatagtatt tgtctttctt catgctgtac atgacgaaag atccactatt tgaaccaaaa
     1201 attttacttt tatctaaaat ttggtaatcg ttgtgtaagg gctttttata attataatca
     1261 gttatggatt gccccatcaa aaactggctt tgaattacca tgagcaaaaa agtaaaaata
     1321 caaataaaaa ttagaattaa tttggactgt tttttcagat tggcttcttt tatattaatt
     1381 aatcttcttt tgagtaatga ctttttacca ttaaatgaat ggcttaatat atttttatta
     1441 aaaaatggaa catttaatac ggagtccata atagattccg cataagtttt aaattcattt
     1501 ttgttaatat tattaaggac aaatctatcg gcttctactt cattgtcatg aattatcttt
     1561 ctttttacaa tatgtactaa tggattgtaa ctcataataa tagagaagat attaaagata
     1621 attaaatgta aagtatctct atttttagca tgagcatatt catgcaatat tatatatttt
     1681 agtcttttgt caattacact tttaaaatat gaactaggaa tcaaaataat atatttccca
     1741 taccaaaaag ttattggaga ttgaatagtc tctgcttttc gaatcacaat atttttttat
     1801 attgatggtt gaaaagtatc gtatctattt tatttttttc attttcgttt agataaagtg
     1861 actgtttctt taaatattta agatataata aggctttcaa aaatttaaaa cttaaaataa
     1921 taactaaaac tatccaaata actgtgcaga tattatcaat tgaatcccaa ttaaacttat
     1981 ggatatctgt tgcgaactct tgaataggtt tggtggtatt tatgttatgg ttcaagtcgt
     2041 gtgacttact ttcaactgtg ggcgcttgat tattcacatt attaaattta aaaagagaga
     2101 atttaatagg aatgaaagga attaatcctg caagaagagt tagataccaa actttataat
     2161 ttaacatata attaaaatag cgttttaata tgtacctaaa aaataataac aatagaaata
     2221 taaaacaaaa gcttactatg ctcattatta ataacttagc catttcaaca ccttctttca
     2281 aatatttata ataaactatt gacaccgata ttacaattgt aatattattg atttataaaa
     2341 attacaactg taatatcgga gggtttattt tgaaaaagtt aatattttta attgtaattg
     2401 ctttagtttt aagtgcatgt aattcaaaca gttcacatgc caaagagtta aatgatttag
     2461 aaaaaaaata taatgctcat attggtgttt atgctttaga tactaaaagt ggtaaggaag
     2521 taaaatttaa ttcagataag agatttgcct atgcttcaac ttcaaaagcg ataaatagtg
     2581 ctattttgtt agaacaagta ccttataata agttaaataa aaaagtacat attaacaaag
     2641 atgatatagt tgcttattct cctattttag aaaaatatgt aggaaaagat atcactttaa
     2701 aagcacttat tgaggcttca atgacatata gtgataatac agcaaacaat aaaattataa
     2761 aagaaatcgg tggaatcaaa aaagttaaac aacgtctaaa agaactagga gataaagtaa
     2821 caaatccagt tagatatgag atagaattaa attactattc accaaagagc aaaaaagata
     2881 cttcaacacc tgctgctttc ggtaagactt taaataaact tatcgcaaat ggaaaattaa
     2941 gcaaagaaaa caaaaaattc ttacttgatt taatgttaaa taataaaagc ggagatactt
     3001 taattaaaga cggtgttcca aaagactata aggttgctga taaaagtggt caagcaataa
     3061 catatgcttc tagaaatgat gttgcttttg tttatcctaa gggccaatct gaacctattg
     3121 ttttagtcat ttttacgaat aaagacaata aaagtgataa gccaaatgat aagttgataa
     3181 gtgaaaccgc caagagtgta atgaaggaat tttaatattc taaatgcata ataaatactg
     3241 ataacatctt atattttgta ttatattttg tattatcgtt gacatgtata attttgatat
     3301 caaaaactga ttttccctct attattttcg agatttattt tcttaattct ctttaacaaa
     3361 ctagaaatat tgtatataca aaaaattata aataatagat gaatagttta attataggtg
     3421 ttcatcaatc gaaaaagcaa cgtatcttat ttaaagtgcg ttgctttttt ctcatttata
     3481 aggttaaata attctcatat atcaagcaaa gtgacaacca taaaaggtga agaacctttt
     3541 tatattcaaa atcaaaaaag cggacatatg aaaagtgaaa aaattccatc tgtcgggttt
     3601 attacagaac caatatgctt tatctaaaga accattatag tacgaaaaaa cacttatgat
     3661 tgtatgtgta cataccctca taagtgctct atttttgtga atggaatttc gcttccatac
     3721 gataaatacg tgacccttta tccgaaaatt tcttttcata ttctgttaaa atattactgc
     3781 catcgtcttc ttgatgtaaa tttagattta tttttgtaaa atacattcca aattgagaca
     3841 tactttctaa actgtaggca aatagtcctc tgttatcagt tttaaagtgt aaatctcctt
     3901 catcatttaa gatttgttga tacaacgcta aaaacgtatg atacgttaaa cgtcgttttg
     3961 catgacgatt ttttggccat ggatctgaaa agttcaaata aatacgcgaa acttcgccgt
     4021 ttttaaaata ttcatttaat tcaatggcat cattacaaat aatctttaaa tttgttaaac
     4081 ccatctcttt aactttatcc aatactttat aaacgatact tttctcacgt tccattgaaa
     4141 tatagttaat atgaggattt tgagcagcta atgttgtaat aaactgcccc atacccgaac
     4201 caatttcaat gtgtatcggt tgcgttttat caaaccattc agtcatttcc cctgcatgtt
     4261 gaccgtccat gtcaaccaat tcaggatgat cttttaaata atcttcagcc catggtttgt
     4321 atcgaactct catattttat tctcctctta aataaacatg ttactattca taacttcatt
     4381 taggaattta agccaagtgt tcatatcctt atatcttttt tgctcttcat accattgaac
     4441 aagacctata gattgaatta ccgtatacca tttcatacgt ttatttaaat tcaagctctc
     4501 ttgaactcca tatgtttcaa gccattcaga ccattgttgt tgtggaacat agttgtaaag
     4561 cagcattccg atatcaattg ccgggtctgc aatcattgca ccttcccaat cgactaaaaa
     4621 tagttcatct cgatcggata ataaccaatt attatgattc acatcaccat gtacaacagt
     4681 gaaaaaacgc gaatctaaac tcggtatatg ctcttctaaa taggttaatg attttctcac
     4741 aatatgatgt gttaaaactt ctcttgataa agaggcatta attttattaa gcataatctc
     4801 aggagtaata ggttccattt ccatacgctt taacatactc aataaaggtc tagaattgtg
     4861 tatcttcttt aataaatgtg caactcttgt ttgcttcatt tcatttgaag atagttcacg
     4921 gccatttttc caatgttgcg ctgtaacaac ctcgcctgtt tctatgcgtt tcgtccatac
     4981 taatttgggc acaatacctt ctgctgataa tgccgcaata aatggatttg aatttcgttt
     5041 taaaaacaac ttttgtccat cttgttcagc catatatgct tcaccagatg caccacctgc
     5101 agaatcaagt gtccatccta attgataaaa ctgctccaac tcgtccacct cactttcaat
     5161 tagaaaatgg ctctagaaat aggtttttca agagccatat attctaattt ataacaccat
     5221 actggtacaa attttatgtc cagataatta ttttaaatcc tcaaccaatg cctacattac
     5281 acgactaaat ttaaatcgta atgtctgtca ttgacaccat acattctata gtcacttact
     5341 tgacataaaa tgttaccgtg tctaaaacta catgtttttg taacttttgt aggcgataaa
     5401 ctctagtttt caaaataatt gctatcccat tttcatggtt aacataaatt tatgaactgt
     5461 aacatttacg tacttagtaa aatatgatgc acatcatatt tgtaactcat agaaaatttt
     5521 ataattttta tcattatatt tcaactgaaa atgagaaaca aaatggcact ttttactaat
     5581 atgtgttttc taaacaacac ttttaagctt cgttttaaat tataacataa ttcacttacg
     5641 aaagttgata aatttaagta atttaatcta aaaatatgat ga
//
LOCUS       DAMZPP010000282         3092 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.guided.201, whole genome shotgun sequence.
ACCESSION   DAMZPP010000282 DAMZPP010000000
VERSION     DAMZPP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.guided.201"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            102..1058
                     /locus_tag="POH73_002844"
     CDS             102..1058
                     /locus_tag="POH73_002844"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDI6888736.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1258..1737
                     /locus_tag="POH73_002845"
     CDS             1258..1737
                     /locus_tag="POH73_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDI6888737.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1752..2591
                     /gene="fakB2"
                     /locus_tag="POH73_002846"
     CDS             1752..2591
                     /gene="fakB2"
                     /locus_tag="POH73_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HDI6888738.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTFNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSVGVAHANVIEYVDKLKKVFNEAFHVDNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2677..>3092
                     /gene="msrA"
                     /locus_tag="POH73_002847"
     CDS             2677..>3092
                     /gene="msrA"
                     /locus_tag="POH73_002847"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDI6888739.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHADNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DKHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYP"
BASE COUNT         1131 a          461 c          541 g          959 t
ORIGIN      
        1 tcatattcct aaaatgagtg ataacaaaat gctacataaa gttaaattat atcgaactaa
       61 atatacatac tataaataat aaaaatgagg tgttatcgca tatgttgaat tcatttgatg
      121 cagcatatca cagtctttgt gaagaagttt tagaaatagg aaatacacga aatgatcgca
      181 caaatacagg tacgatttcg aaatttggtc atcaacttcg ctttgactta tctaaaggat
      241 ttccactatt aacgacaaag aaagtttctt ttaaattagt agcgaccgaa ttattatggt
      301 tcattaaagg agatacaaat atccaatact tattaaagta taataataat atttggaacg
      361 aatgggcttt tgaaaattat atcaaatcag acgagtataa aggtccagat atgacagatt
      421 tcgggcatcg tgcattgagt gatcctgaat ttaacgaaca atataaagaa caaatgaaac
      481 aatttaagca acgtattctt gaagatgata catttgcgaa gcaattcggt gatttaggaa
      541 atgtttatgg taaacagtgg cgagattggg ttgataaaga tggtaatcat tttgatcaac
      601 ttaaaacagt aattgagcaa attaagcata atccagattc aagacgacac atcgtatctg
      661 catggaatcc aacagaaatt gatacaatgg cacttccgcc ttgtcataca atgttccagt
      721 tttatgttca agatggtaag ttaagttgcc agttatacca acgtagcgca gatatctttt
      781 taggtgtgcc atttaatatc gcaagctacg ctttattgac gcaccttatt gccaaagaat
      841 gtggacttga agtaggagaa tttgtgcata catttggaga tgcacatatt tattcaaatc
      901 atattgatgc gattcaaaca caattagcac gtgaaagctt caatcctcca acattaaaaa
      961 ttaacagtga taagtctatt tttgacataa attatgaaga tttggaaatt gttgactatg
     1021 aatcacatcc agcaataaaa gctccaatag cagtgtagtc gttgcatagt tagctaacca
     1081 tatagacatc aaaatgacat caaagtattt tcaagtgcaa aaaagtactt ttttgtgtta
     1141 aacgttttca taaattatgc aaaatcatta tttctatcac actttatgat aaaaattgtg
     1201 ttaaattaaa gataacttag taattaaaaa tgaaatgata gaagaaggag gataatcatg
     1261 actttatcca ttctagttgc acatgacttg caacgagtaa ttggttttga aaatcaatta
     1321 ccttggcacc taccaaatga tttgaagcat gttaaaaaat tatcaacagg tcatacttta
     1381 gtaatgggtc gtaagacatt tgaatcgatt ggtaaaccac taccgaatcg tcgaaatgtt
     1441 gtacttactt cagatacaag tttcaacgta gagggcgttg atgtaattca ttctattgaa
     1501 gatatttacc aactaccggg ccatgttttt atatttggag ggcaaacatt atttgaagaa
     1561 atgattgata aagtggacga catgtatatt actgttattg aaggtaaatt tcgtggtgat
     1621 acgttctttc caccttatac atttgaagac tgggaagttg cctcttcagt tgaaggtaaa
     1681 ctagatgaga aaaatacaat tccacatacc tttctacatt taattcgtaa aaaataaggg
     1741 ggaaaacgac catgacaaaa cagattatag taacagactc aacatccgat ttatctaaag
     1801 aatatttaga agcaaacaac atacatgtaa ttcctttaag tttaactatt gaaggagctt
     1861 catacgttga ccaagtagat attacatcag aagaatttat taatcatatt gaaaatgatg
     1921 aagatgtaaa gacaagtcag ccagccatag gtgaatttat atctgcttat gaagaactag
     1981 gaaaagatgg ttctgaaatc ataagtattc atctttcttc aggactaagt ggtacattta
     2041 acactgctta ccaagcaagt caaatggtag atgctaatgt aactgttatt gattcaaaat
     2101 ctatttcttt tggtttaggg tatcaaatac aacacctagt agagcttgta aaagaaggtg
     2161 tctcaacttc tgaaatagtt aaaaagttaa atcatttaag agaaaacatt aaattatttg
     2221 tagttatagg gcaattgaat caattaatta aaggtggcag aattagtaaa acaaaaggtt
     2281 tgattggtaa tcttatgaaa attaaaccaa ttggtacact agatgatggt cgcttagagc
     2341 ttgtgcacaa tgcgagaact caaaattcta gtatccaata cttgaaaaag gaaattgctg
     2401 aatttatagg agatcatgaa atcaaatccg ttggtgtcgc acatgctaac gtcattgaat
     2461 atgttgataa attgaagaaa gtttttaatg aagcttttca tgtggataat tacgatataa
     2521 atgtaactac accagttatt tctgcacata ctggtcaagg tgcgattggc ctcgtagtcc
     2581 ttaagaagta aatttaatct tttcagtgtt aattacttcc atttcaatcc tttatagact
     2641 aaatttataa ttagatagat agaggaggta attcatatga caaaagaata tgcaacatta
     2701 gcaggaggat gtttctggtg catggttaaa ccatttacat catatccagg catcaagtca
     2761 gtcgtatctg gttatagtgg cggtcatgct gataacccaa cttatgaaca ggtatgtacg
     2821 aatcaaaccg gccatgtcga agcagtacaa attacgtttg atccagaggt tacttccttt
     2881 gaaaatatat tagacatata tttcaaaaca tttgacccaa ccgatgatca agggcaattt
     2941 ttcgatagag gcgaaagcta tcaaccagtc attttctatc atgataagca tcagaaaaag
     3001 gctgctgagt ttaaaaagca acaattaaat gaacaaggta ttttcaagaa accagtgatt
     3061 acacctatta aaccatataa aaatttctat cc
//
LOCUS       DAMZPP010000283         1788 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.guided.233, whole genome shotgun sequence.
ACCESSION   DAMZPP010000283 DAMZPP010000000
VERSION     DAMZPP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.guided.233"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..288
                     /locus_tag="POH73_002848"
     CDS             <1..288
                     /locus_tag="POH73_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDI6888740.1"
                     /translation="VETARDKGVRHHVGNVLSSDIFYNADTTASERWMRMGILGVEME
                     SAALYMNAIYAGVEALGVFTVSDHLIHETSTTPEERERAFTDMIEIALSLV"
     gene            295..1647
                     /gene="tet(38)"
                     /locus_tag="POH73_002849"
     CDS             295..1647
                     /gene="tet(38)"
                     /locus_tag="POH73_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDI6888741.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMRIVGLVLFVILNRWTQSEK
                     "
BASE COUNT          557 a          266 c          324 g          641 t
ORIGIN      
        1 gttgaaacag cacgtgacaa aggtgtacgt catcatgtag gtaatgtgtt atcaagtgat
       61 attttctata acgcggatac aacagcgagt gaacgttgga tgcgtatggg tattttaggt
      121 gtagaaatgg aatcagctgc attatacatg aatgcaattt acgctggtgt cgaagcatta
      181 ggtgtgttca cagtgagcga tcatttaatt catgaaacgt caacaacacc tgaggaaagg
      241 gaacgtgcat ttacagatat gattgaaatt gcactgtcat tggtgtagat gattatgaat
      301 gttgaatatt ctaaaataaa gaaagcagta cctattttat tattcttatt tgtattcagt
      361 ttggttatag acaactcatt caaattgatt tctgtagcca ttgctgatga cttaaacatt
      421 tcagtaacga cagtaagttg gcaagcgaca ttagccggtt tagtaattgg tattggcgct
      481 gtagtatacg cttcattatc tgatgccatt agtatacgca cactatttat ttatggcgtg
      541 atattaatca ttatcggatc aattattggt tacattttcc aacatcaatt cgcattactt
      601 ttagttggac gtattattca aactgcaggt ttagctgctg cagagacatt atatgtgata
      661 tatgttgcaa agtatctttc taaagaggac cagaagactt accttggctt aagtacgagc
      721 agttattcct tgtcattagt tatcggtaca ttatcaggtg gatttatttc tacgtattta
      781 cactggacaa atatgttttt aattgcatta atcgtagtat ttacgttgcc attcctattt
      841 aaattattac caaaagaaaa taatacaaat aaagctcatt tagattttgt tggcttaatt
      901 ctagtggcaa ctatcgctac aacagttatg ctgtttatta cgaactttaa ttggttatat
      961 atgattggtg ccttaattgc gattatcgtt tttgcgctat atattaaaaa tgcgcaacgt
     1021 ccattagtaa acaaatcatt tttccaaaat aaacgttatg cttcattttt atttatagta
     1081 tttgtaatgt atgccatcca attgggttat atttttacgt tcccattcat aatggaacaa
     1141 atttatcatc ttcagctaga cacaacatca ctgctattag taccgggtta tattgtagca
     1201 gtcatcgttg gtgcactaag tggtaaaatc ggcgaatatc ttaattcaaa acaagcgatt
     1261 atcacagcaa ttattttaat cgcactgagc ttgattttac ctgcatttgc agtaggtaat
     1321 cacatttcaa tcttcgtcat ttctatgata ttctttgcag gtagctttgc tttaatgtat
     1381 gcacctttac ttaacgaagc cattaaaaca atagatctta atatgacagg tgtggctatt
     1441 ggtttttata atttaattat taatgtggcg gtatctgtag gtattgcgat tgctgcggct
     1501 ttaatcgatt ttaaagcatt aaatttccca ggcaatgatg cattaagttc acatttcggt
     1561 attattttaa ttattttagg tttaatgagg attgtcggat tagttttatt cgtcatctta
     1621 aatcgttgga cacaatctga aaaataaata gatataaatt cgcgagatat attcgtattt
     1681 atagtaaaat taaataaaga gattatataa cacgaggagt agtaagtatg aaatttgaga
     1741 aatatataga tcacacttta ttgaagcctg agtcaacacg tacgcaaa
//
LOCUS       DAMZPP010000284         1788 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.guided.234, whole genome shotgun sequence.
ACCESSION   DAMZPP010000284 DAMZPP010000000
VERSION     DAMZPP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.guided.234"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            <1..288
                     /locus_tag="POH73_002850"
     CDS             <1..288
                     /locus_tag="POH73_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDI6888742.1"
                     /translation="VETARDKGVRHHVGNVLSSDIFYNADTTASERWMRMGILGVEME
                     SAALYMNAIYAGVEALGVFTVSDHLIHETSTTPEERERAFTDMIEIALSLV"
     gene            295..1647
                     /gene="tet(38)"
                     /locus_tag="POH73_002851"
     CDS             295..1647
                     /gene="tet(38)"
                     /locus_tag="POH73_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDI6888743.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
BASE COUNT          557 a          266 c          323 g          642 t
ORIGIN      
        1 gttgaaacag cacgtgacaa aggtgtacgt catcatgtag gtaatgtgtt atcaagtgat
       61 attttctata acgcggatac aacagcgagt gaacgttgga tgcgtatggg tattttaggt
      121 gtagaaatgg aatcagctgc attatacatg aatgcaattt acgctggtgt cgaagcatta
      181 ggtgtgttca cagtgagcga tcatttaatt catgaaacgt caacaacacc tgaggaaagg
      241 gaacgtgcat ttacagatat gattgaaatt gcactgtcat tggtgtagat gattatgaat
      301 gttgaatatt ctaaaataaa gaaagcagta cctattttat tattcttatt tgtattcagt
      361 ttggttatag acaactcatt caaattgatt tctgtagcca ttgctgatga cttaaacatt
      421 tcagtaacga cagtaagttg gcaagcgaca ttagccggtt tagtaattgg tattggcgct
      481 gtagtatacg cttcattatc tgatgccatt agtatacgca cactatttat ttatggcgtg
      541 atattaatca ttatcggatc aattattggt tacattttcc aacatcaatt cgcattactt
      601 ttagttggac gtattattca aactgcaggt ttagctgctg cagagacatt atatgtgata
      661 tatgttgcaa agtatctttc taaagaggac cagaagactt accttggctt aagtacgagc
      721 agttattcct tgtcattagt tatcggtaca ttatcaggtg gatttatttc tacgtattta
      781 cactggacaa atatgttttt aattgcatta atcgtagtat ttacgttgcc attcctattt
      841 aaattattac caaaagaaaa taatacaaat aaagctcatt tagattttgt tggcttaatt
      901 ctagtggcaa ctatcgctac aacagttatg ctgtttatta cgaactttaa ttggttatat
      961 atgattggtg ccttaattgc gattatcgtt tttgcgctat atattaaaaa tgcgcaacgt
     1021 ccattagtaa acaaatcatt tttccaaaat aaacgttatg cttcattttt atttatagta
     1081 tttgtaatgt atgccatcca attgggttat atttttacgt tcccattcat aatggaacaa
     1141 atttatcatc ttcagctaga cacaacatca ctgctattag taccgggtta tattgtagca
     1201 gtcatcgttg gtgcactaag tggtaaaatc ggcgaatatc ttaattcaaa acaagcgatt
     1261 atcacagcaa ttattttaat cgcactgagc ttgattttac ctgcatttgc agtaggtaat
     1321 cacatttcaa tcttcgtcat ttctatgata ttctttgcag gtagctttgc tttaatgtat
     1381 gcacctttac ttaacgaagc cattaaaaca atagatctta atatgacagg tgtggctatt
     1441 ggtttttata atttaattat taatgtggcg gtatctgtag gtattgcgat tgctgcggct
     1501 ttaatcgatt ttaaagcatt aaatttccca ggcaatgatg cattaagttc acatttcggt
     1561 attattttaa ttattttagg tttaatgagt attgtcggat tagttttatt cgtcatctta
     1621 aatcgttgga cacaatctga aaaataaata gatataaatt cgcgagatat attcgtattt
     1681 atagtaaaat taaataaaga gattatataa cacgaggagt agtaagtatg aaatttgaga
     1741 aatatataga tcacacttta ttgaagcctg agtcaacacg tacgcaaa
//
LOCUS       DAMZPP010000285          448 bp    DNA     linear   BCT 28-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate 641
            SAMN20442202-rid16885023.guided.265, whole genome shotgun sequence.
ACCESSION   DAMZPP010000285 DAMZPP010000000
VERSION     DAMZPP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20442202
            Sequence Read Archive: SRR15275091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001592633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001592633.1
            Genome Coverage       :: 20x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 21:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,839
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 13
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 7
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 58 of 89
            Pseudo Genes (incomplete)         :: 13 of 89
            Pseudo Genes (internal stop)      :: 30 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20442202-rid16885023.guided.265"
                     /isolate="641"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="USA"
                     /collected_by="Children's Hospital of Philadelphia"
     gene            2..421
                     /gene="fosB"
                     /locus_tag="POH73_002852"
     CDS             2..421
                     /gene="fosB"
                     /locus_tag="POH73_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB/FosD family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HDI6888744.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFKLAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
BASE COUNT          174 a           62 c           71 g          141 t
ORIGIN      
        1 gatgttaaaa tctattaatc atatatgctt ttcagtcaga aatttaaacg attcaataca
       61 tttttataga gatattttac ttggaaaatt gctattgact ggtaaaaaaa ctgcttattt
      121 taagcttgca ggcctatgga ttgctttaaa tgaagaaaaa gatataccac gtaatgaaat
      181 ccacttttca tatacacata tagctttcac tatagatgac agcgaattta aatattggca
      241 tcagaggtta aaagataata acgtgaatat cttagaagga agagttagag atattagaga
      301 tagacaatca atttacttta ctgaccctga tggtcataag ctagaattac atactggcac
      361 acttgagaac agattaaatt attataaaga ggctaaacca catatgacat tttacaaata
      421 aggcgtcatt ataaaaaggc ctcttgaa
//
